LOCUS       DATGOU010000001        10851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_2666638, whole genome shotgun sequence.
ACCESSION   DATGOU010000001 DATGOU010000000
VERSION     DATGOU010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10851
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2666638"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1541
                     /gene="rpoD"
                     /locus_tag="VKZ48_00005"
     CDS             <1..1541
                     /gene="rpoD"
                     /locus_tag="VKZ48_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011658300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HLU76264.1"
                     /translation="LTREGEIEIAKRIEEGLRHMIQAISACPTTIEEILELADKVKHD
                     EMRIDELVDGLVDPDAEEEEVPQPAIVNRAAATSNTSNDADGESDDEDGDDDADDGAA
                     AIAANLQQLKEDSLEIFQRIHKLNDKMKQALLKHGHRSKEYVTLRDKIGEELMYIRFT
                     ARIIERLSDSVRSMVEQVRRHEREIQHLVVVKGGMPRSHFIKLFPNNETSPRWLKSEI
                     AAGHPYSETLARVEPNVLEQQEKLLEIQNKIGIPLKDLKDINRKMSTGEAKARRAKRE
                     MTEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIR
                     QAITRSIADQARTIRIPVHMIETINKMNRISRQILQETGQEPDPATLAEKMEMPEDKI
                     RKIMKISKEPISMETPIGDDDDSHLGDFIEDTTTLAPNDAALYASLREVTKDILDSLT
                     PREAKVLRMRFGIEMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSERLKSF
                     LDNS"
     gene            1777..1853
                     /locus_tag="VKZ48_00010"
     tRNA            1777..1853
                     /locus_tag="VKZ48_00010"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1811..1813,aa:Ile,seq:cat)
     gene            2182..3447
                     /gene="oxlT"
                     /locus_tag="VKZ48_00015"
     CDS             2182..3447
                     /gene="oxlT"
                     /locus_tag="VKZ48_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HLU76265.1"
                     /translation="MQLLLGLIAMMAISSPQYVWALYTQHLTASLGATLAEIQVTFSV
                     LIVLQTFLSPLQGFLIDKFGPKRLLSVGAALTGLSWIATAYVDSLWGLYLTYGMMGGI
                     GTGIIYVGVVGMMVQWFPDYRGFATGVVAAGYGFGAVVTTLPISRSLSSQGFEQTLIL
                     YGFIIGAVGILAALGMRRPNGNGVPSKALAAAAANPAVVETVRSFTSKEMLRNPIFWL
                     LFLMMTMMSTTGLMVISQMGAIARDFNVADALVFGLAALPLALTVDRITNGLTRPFFG
                     WVSDKIGRENTMFIAFSLEGAAITVWYFMAHDPFWFVVLSGVVFFGWGEIFSLFPSIL
                     TDTFGTRHATTNYGFLYMAQGVGSILGGPMAAMLYASTGSWGAVFTVVVAMDIITAIL
                     AIAVLKPMRSRHLQANAVNNAVLAAAAPA"
     gene            3664..4398
                     /locus_tag="VKZ48_00020"
     CDS             3664..4398
                     /locus_tag="VKZ48_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLU76266.1"
                     /translation="MSARKSRKNKPQRLIEGETQAQYAHRVIEEMIVTLALPPGSRIS
                     EQYLSDQLGIGRTPVREALQRLAYERTIQILPRSGAVVSPIDISEQFKLIEVRRELER
                     IMVTRAARLIDPVARRKFLDLEERFRRAEETDDASIFIPADREFNLLLARTADNDYAA
                     AAMAPLQAQTRRLWFLYFKHFGDVSRVAGLHADIARAIADKDEQRARAASDALVDYAE
                     DYTYRTIEAVQNETAKEESGATRQVA"
     gene            complement(4480..6084)
                     /gene="ggt"
                     /locus_tag="VKZ48_00025"
     CDS             complement(4480..6084)
                     /gene="ggt"
                     /locus_tag="VKZ48_00025"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HLU76267.1"
                     /translation="MAVKTTRPVAMATGGMVASPHYFASLAGLRVLQEGGSAVDAAIA
                     VNSTLGIVYPHMTGMGGDAFWLIYDAKQNKVHALNGSGPAVSGATRAHYRKLGLNNIP
                     QRGPHAALTVPGAVDAWCKAHERFGKLSMAQCLQPAIHYARNGYPVSAGQVQFTRGTA
                     QILGRYADTRDAFMPGGRAPELGEIMRFPGMADTMEAIAKNGRAGFYEGPVAEEIVRA
                     LTAAGGDWQTSDLSGFHSEWGEPISTDYRGYKCYQHPPNSQGFAHLMTLNILENFDVA
                     ALRTDRAAYIHLLVEATKLAFMDRDRYLTDPRHNDIPLDRLLAKAYAKELAARIDFDQ
                     AMAHEPNPMGQDTTCTVVVDGEGNAASIIQSLYHEFGSCFVAGKTGILLQNRGSFFSL
                     DDDHINRLEPGKRSFHTLMPGMLFRDGKLDLVYGTMGGEGQPQTSTAVVTRYVDFNHD
                     VQTAVDNPRWLYGRTWGDATRALRLENRFDEEVFASLRARGHEVQITEAWCDFMGHAA
                     AIKVDQRTGVLFGAADARGEGIAVGW"
     gene            6306..6473
                     /locus_tag="VKZ48_00030"
     CDS             6306..6473
                     /locus_tag="VKZ48_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76268.1"
                     /translation="MVVAMHILRTTAQLLPLRRLTVPDGACACAVATAAAARQSDIAL
                     ADPAFPAFPGN"
     gene            6585..6989
                     /locus_tag="VKZ48_00035"
     CDS             6585..6989
                     /locus_tag="VKZ48_00035"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HLU76269.1"
                     /translation="MRTTESYVTKQDANLLRTLFQRPDLEAVRRGGAMNPLIDALREA
                     RVVDTVPATRVRLGSTVRYIDERTGSTNLVTLTLPKDANTAPQHLSVLSPVATALLGR
                     AVGERIEVSLPGDRTTALRIVEVEQEEALSEA"
     gene            7148..8224
                     /locus_tag="VKZ48_00040"
     CDS             7148..8224
                     /locus_tag="VKZ48_00040"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLU76270.1"
                     /translation="MSLNVDRSLPPDAAHPSPPPGGGPVPCALLESAMLPVGDRFDVW
                     RESVLPLFESIPDEAPDNFHARVESYDLRHLVMAMSAFSPLRFHRDRHHRVAEAADHL
                     LVQLYVEGGYVGHNGYREVCVRPGDISLLDLGRELETRAPASNALSLVIPREQLYAYA
                     SPERLTHSSVLRGDSVVGSVLGHHLLTVWGKLPSACVEEIDDINHLLLSSIVGAFTPA
                     PTDPSGLPERITQEAIRAYIAQNLTEPLTPELLCHRFACSRAQLYRLFQPFGGVAAYI
                     RDARLNRCWQELTERSDRPKRIGDVAMRWGFTSQSHFNRLFRQAFGVTPGEAVERAQS
                     HVGAGSTAPHADTRPSLRDLLRRL"
     gene            8311..10008
                     /locus_tag="VKZ48_00045"
     CDS             8311..10008
                     /locus_tag="VKZ48_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HLU76271.1"
                     /translation="MDRPSTLIAATRCNAALDALLNCAAEGIFGLDRLGRCTFCNASA
                     ARMLGYDSPAELVGQRMHSLIHAMREDGSPCVAAQCAMNRAFLEHRSAHDDTAYMWRR
                     GGSAFPVEYWTAPLLENGTVVGTVVSFIDITERKASAHALQHQATHDVLTGLQNRATL
                     HGAIRSAIEAADGERGIAIALIDLDRFKQVNDTLGHSVGDQVLVELAVRLRQAAGPQN
                     TLVRLGGDEFAVVIGDVAHEREAQTVAERLLNKLISPVEVAGMSLRIGASIGIAVYPE
                     HGEDSSALLRKADIAMYVAKRHGAGYKFYQPERDTGASARHALLVELRDAMSADQLHL
                     RFQPQLSLATRQAVAFEAMPHWQHPQRGLLPPEEFMPLVGLSDLVQPLALRMIDQALA
                     QCRQWHLAGHPFNVAIQIPSRCLLDQTLPQRIEALLAKHELPPHLLELQMRGGAMADT
                     RRAMEILHVLGGLGIGVVIDDFGSAYGSLRDLARLRLRGVRIDCAGLHSDDEAKIIAT
                     AAAMAHSLGACVIAKGVQKECALADLQKLGCDRIQGESIAPPLTADEIGPWLQRNGG"
     gene            10144..>10851
                     /locus_tag="VKZ48_00050"
     CDS             10144..>10851
                     /locus_tag="VKZ48_00050"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76272.1"
                     /translation="MQSPTADPLLLLSTDALDPHVRYDYWREMVFCYFDAVPDAPDAV
                     QRFESHVVSLISERGEINSYRSSDVTGQRTRRQYQRDGGDEVALIYVLKGKAVYQPEN
                     GVPTEAAAGKFFCYDSARPARVQWSAGEQLHFSLPRAAVMEAIGGDVPEPDVLARSLN
                     ESRLAPYLLSQIRRLTREAPQLSPFERATMLDGTLELAKAVLRGSLHPARAFADGETA
                     LYYSACTHIDAHLGKPDL"
BASE COUNT         1951 a         3438 c         3453 g         2009 t
ORIGIN      
        1 tgctgacccg cgaaggcgag atcgaaatcg ccaagcggat cgaagagggc ctgcgtcaca
       61 tgatccaggc gatttccgcc tgcccgacca cgatcgagga aatcctcgag ctggccgaca
      121 aggtcaagca cgatgagatg cgcatcgacg aactggtcga cggcctggtc gaccccgacg
      181 cggaagagga agaagtgccg cagccggcga tcgtcaatcg cgccgctgcg accagcaaca
      241 cgtccaacga tgccgacggc gagtcggacg acgaggacgg cgacgacgat gccgacgacg
      301 gcgccgctgc catcgcggcc aacctgcagc agttgaaaga ggattcgttg gaaatcttcc
      361 agcgtatcca caagctgaac gacaagatga agcaggcgct gctcaagcat ggccaccgct
      421 cgaaggagta cgtcacactt cgcgacaaga tcggcgagga gttgatgtac atccggttca
      481 ccgcgcgcat catcgaacgg ctgtccgaca gcgtgcggag catggtcgag caggtacgtc
      541 gccacgagcg cgaaatccag catctcgtgg tcgtcaaggg tggcatgccg cgctcgcatt
      601 tcatcaagct tttccccaac aacgaaacca gtccgcgttg gttgaagagc gagattgccg
      661 ccggccatcc ctattccgag acgctcgcgc gcgtcgagcc gaacgtgctg gagcagcagg
      721 aaaaactgct ggagatccag aacaagatcg gtattccgct gaaagacctc aaggacatca
      781 acagaaagat gtccaccggc gaggcgaagg cgcgtcgcgc caagcgcgag atgaccgagg
      841 ccaacctgcg tctggtgatc tcgatcgcga agaagtacac gaaccgcggc ctgcagttcc
      901 tcgacctcat ccaggaaggc aacatcggcc tgatgaaggc ggtggacaag ttcgaatatc
      961 gtcgcggcta caagttctcg acgtatgcga cctggtggat tcgtcaggcc atcacgcgtt
     1021 cgatcgccga ccaggcgcgc accatccgta tcccggtgca catgatcgag acgatcaaca
     1081 agatgaatcg catctcgcgc cagatcctgc aggagaccgg gcaggagccg gatcccgcga
     1141 cgctggccga gaagatggag atgcccgagg acaagatccg caagatcatg aagatctcca
     1201 aggagcccat ttccatggag acgccgatcg gcgacgacga cgattcgcac ctgggcgatt
     1261 tcatcgagga tacgacgacg ctggcgccga acgatgccgc gctgtacgcc tcgctgcgtg
     1321 aagtgaccaa ggacatcctg gattcgctca cgccgcgcga agcgaaggtg ctgcgcatgc
     1381 gcttcggcat cgagatgaac accgaccata cgctggaaga ggtcggcaag cagttcgacg
     1441 tcacgcgcga acgcattcgc cagatcgaag cgaaggcgct gcgcaagctg cgtcatcctt
     1501 cgcgctccga acgcctcaag tccttccttg acaacagtta aagcgcgcag cgctttaact
     1561 gttgcggcga aggctaactt tgcgcaacgc gcgaagttaa gggcgcagcc cggccggagg
     1621 cacaacaccg atagcgcagc gctttaactg ttgcggcgaa ggcgagcttt gcgtcgcgtg
     1681 cgaacgaaag cttcgtggtc cacgttaaag aaaacgcccc ttcgggggcg ttttcttttt
     1741 ccgcagggcg gggcacgcta gaatgcccat ccttgtgggc ctatagctca gtcggttaga
     1801 gcagaggact cataatcctt tggtccctgg ttcgagtcca ggtgggccca ccacaatgct
     1861 actggcggtc tgtcagaccg ctcgctttgc tacgacacgt tccgtccggc gacgccacgc
     1921 ctcaactcct gcttattgct cgccgtagac cttcatttcc cacccgcctc ctccctctat
     1981 ttgtccatgg caaatagtat actagtattc attactggta tgcggtaaag tcaaatcgta
     2041 tgtcagatga gccggggagg gccggcttat cacgcccggt gcagcggtgc ggggcgaaga
     2101 cagatcgagc aactgtcata aggaggaggt ggcatgccac aagcattggg cgggacaacg
     2161 gccgcaagca ggacgaggtg gatgcagttg ctgctgggtt tgatcgcaat gatggcgatt
     2221 tcgagcccgc agtatgtctg ggcgctttat acgcagcatt tgacggccag tctgggcgcg
     2281 acgctcgcgg agattcaggt gactttctcc gtgctgatcg tgctgcagac ttttttgtcg
     2341 cctttacagg gattcctgat cgacaagttc ggtcccaaac gcctgctttc cgtgggcgcg
     2401 gcgttgaccg gtctgagctg gattgcgacg gcatacgtcg attcgctctg gggactgtac
     2461 ctgacctacg gcatgatggg cggcatcggt accggcatca tttacgtcgg cgtggtcggc
     2521 atgatggtgc agtggttccc ggattaccgg ggatttgcga ccggcgtggt cgcggccggc
     2581 tacggcttcg gcgcggtggt gacgaccttg ccgatttccc gctcgctgtc ctcacagggt
     2641 ttcgaacaaa cgctgattct gtacggcttc atcatcggtg ccgtcggcat tctcgcggcc
     2701 ctgggcatgc gccgaccgaa cggtaacggc gtaccgagca aggccttggc tgccgcagcg
     2761 gccaatccgg cggtggtcga gacggtacgc agctttacgt cgaaggagat gctgcgcaat
     2821 ccgatcttct ggctgctctt tctgatgatg accatgatgt ccaccaccgg cctgatggtg
     2881 atctcgcaaa tgggcgccat cgcccgcgac ttcaacgtgg cggacgcgct ggtgttcgga
     2941 cttgcggcgc tgccgctggc cctgacggtg gatcgcatca ccaacggcct cacccgaccg
     3001 ttcttcggct gggtgtcgga caagatcggg cgggagaaca ccatgttcat cgccttctcg
     3061 ctggaaggcg cggccatcac ggtgtggtac ttcatggcgc acgatccgtt ctggttcgtc
     3121 gtgctgtccg gggtcgtgtt cttcggctgg ggcgagatct tctcgctgtt tccctcgatc
     3181 ctgaccgata ccttcggcac caggcacgcc acgacgaact acggcttcct gtacatggcg
     3241 cagggtgtcg gctccatcct cggcggcccg atggcggcga tgctgtacgc cagcaccggc
     3301 tcctggggcg cggtgttcac cgtcgtcgtc gcgatggaca ttattacggc gatcctggcc
     3361 atcgcggtgc tcaagccgat gcgcagccgc catctccagg cgaatgcggt gaataacgcc
     3421 gttctggctg ctgctgcccc ggcctgatcg ggcgttgtga gaacaagcgg tacgaggctt
     3481 ctacggcctc gtaccgcttg ctgcgttgtg gtagccggga tgacattgcg gcattcccat
     3541 gcgaaaggtt gttgcaatgc atcaagcttc caccccaaag gcctgagctt ggcctacact
     3601 tcccccggaa cggcccgaaa tccgtcggcc ataaggggat ggttggtgtc ggggaatgat
     3661 tgaatgagcg ctcgcaaatc tcgcaagaac aagcctcagc gtctgatcga aggcgaaacc
     3721 caggcgcagt acgcgcaccg cgtcatcgag gagatgatcg tcacgctggc attgccgcca
     3781 ggcagccgca tttccgaaca gtatctgagc gaccagctcg gcatcggccg cacgccagtg
     3841 cgtgaggcgc tacagcgact ggcgtatgag cggacgattc agatcctgcc gcgctccggc
     3901 gcggtcgttt cccccatcga catatccgag caattcaagc tcatcgaagt ccgccgcgag
     3961 ctggaacgca tcatggtgac gcgggccgcg cgtctgatcg accccgtggc gcgccgcaaa
     4021 ttcctggatc tggaggagcg gttccgccgc gcagaggaaa ccgacgacgc ctcgatcttc
     4081 attccggcgg atcgcgagtt caacctgctt ctcgctcgca ccgccgacaa cgactacgcc
     4141 gccgctgcca tggcgcccct gcaggcccag acccggcggt tatggttcct gtacttcaag
     4201 catttcggcg acgtttcacg cgtggcgggt ctgcacgccg atatcgcgcg agccatcgcc
     4261 gataaggacg aacagcgtgc gcgcgccgcg tccgatgcgc tggtggatta cgccgaggac
     4321 tacacatacc gcaccatcga ggcggtgcag aacgaaaccg ccaaagagga atcaggcgcc
     4381 acgcgccagg ttgcctgagc gcgtcatcgt atcgtcgtat cggcggcgca aaaaaaacgg
     4441 cgcaccgggc cagcgtgcgc cgaatcggga ggagttccct taccagccga cagcgatgcc
     4501 ctcgccgcgc gcatccgccg cgccgaacag cacgccggtc cgctgatcga ccttgatcgc
     4561 cgctgcgtgg cccatgaaat cgcaccacgc ttcggtgatc tgcacctcgt gcccgcgtgc
     4621 gcgcagcgaa gcgaaaacct cctcgtcgaa gcggttctcg aggcgcaggg cacgtgtcgc
     4681 atcgccccag gtgcggccgt acagccagcg tgggttgtcc accgccgtct gcacgtcgtg
     4741 attgaagtcc acgtagcgcg tcaccaccgc ggtgctcgtc tgcggctggc cttcgccgcc
     4801 catcgtgccg tacaccagat ccagcttgcc gtcgcggaac agcatgccgg gcatcagggt
     4861 gtggaaggag cgcttgcccg gttccaggcg gttgatatgg tcgtcgtcca gcgagaagaa
     4921 cgagccgcgg ttctgcagca ggatgccggt cttgccggcg acgaaacacg agccgaactc
     4981 gtggtacagg ctctggatga tcgatgcggc gttgccttcg ccgtccacca ccaccgtgca
     5041 ggtcgtgtcc tgtcccatcg gattcggttc atgcgccatg gcctgatcga agtcgatgcg
     5101 cgcggccagt tccttcgcat aagccttcgc gagcagccgg tcgagcggga tgtcgttgtg
     5161 tcgcggatcg gtcagatacc ggtcgcggtc catgaaggcg agcttggtcg cttcgaccaa
     5221 tagatggatg tacgctgcgc ggtcggtgcg cagcgccgcc acatcgaagt tctccaggat
     5281 gttgagcgtc atcaggtgcg cgaagccctg actgttgggc ggatgctgat agcacttgta
     5341 gccgcggtag tccgtgctga tcggctcgcc ccactcgctg tggaagccgc tcaggtcgct
     5401 ggtctgccaa tcgccgcccg cggcggtgag tgcgcggacg atctcctcgg ccaccggtcc
     5461 ctcgtaaaaa ccggcgcggc cgttcttggc gatcgcttcc atggtgtccg ccataccggg
     5521 aaagcgcatg atctcgccca actccggcgc gcggccgccg ggcatgaagg cgtcgcgcgt
     5581 gtcggcgtag cgtccgagga tctgtgcggt gccgcgcgtg aactgcacct gaccggcgga
     5641 caccgggtag ccgttgcgcg cgtagtggat cgccggttgc agacactgcg ccatggagag
     5701 cttgccgaag cgctcgtgcg ccttgcacca ggcatcgact gcgccgggaa cggtgagggc
     5761 ggcatgcggg ccacgctgcg ggatattgtt cagccccagc ttgcggtaat gcgcgcgcgt
     5821 ggcgccggag accgccggac cggaaccgtt cagggcatgc accttgtttt gtttggcgtc
     5881 gtagatcagc cagaacgcat cgccgcccat ccccgtcatg tgcgggtaga cgattcccag
     5941 cgttgaattc accgcgatgg ccgcgtcgac agcacttccg ccttcctgca ggacgcgcag
     6001 tcccgcgagc gaggcgaagt agtgcggcga ggcgaccatg ccgcctgtcg ccattgccac
     6061 cggtcgggtc gttttcaccg ccatcccacc ctcctttgcg atatactatg ttgtaatctg
     6121 atatgtcaga ctaacacagc ccgcgccggt tcggtaccca aaaaaccgca ttcagcctcg
     6181 attgcggtgc gaatcggata actaagcggg caactgcccc cgggccgata tgacagccgc
     6241 cgcgcctgac ccggccctcg cgctctaagg tattgtcgcg gctggatttt tcgtgcagat
     6301 gtgctatggt ggtggccatg cacatcctcc gcaccaccgc ccagcttctg cccctgcgcc
     6361 gtctcacggt gccggatggg gcgtgtgcgt gcgccgtcgc gaccgccgct gctgcgcggc
     6421 agtcggacat cgcattggcc gatccggcct tccccgcttt ccccggtaat tagcgacctc
     6481 cgcgctgcgg cgtcggccgc agttaccggg ttcaaccatt caccagctcg atgctcgcgc
     6541 gctgcgcgac gcatcgctgt tgccattttc cgaggaggga atgtatgaga acgactgaaa
     6601 gctatgtgac caagcaggac gccaacctgt tgcgtacctt gttccagcgg cccgatctgg
     6661 aggccgtgcg gcgtggcggt gccatgaacc cgctcatcga cgcactgcgc gaagcgcgcg
     6721 tggtcgatac cgtgcccgcc acgcgggtgc ggctgggctc gaccgtccgc tatatcgacg
     6781 agcgcacggg ttcgaccaat ctcgtcaccc tgacgctgcc caaggatgcg aacaccgcgc
     6841 cgcagcacct ctccgtgctg tcgccggtcg cgaccgcgct gctgggccgc gcggtgggcg
     6901 agcggatcga agtgagcttg ccgggtgacc gcacgacggc gttgcgcatc gtagaagtcg
     6961 agcaggaaga agcgctcagc gaagcctgac cggacgtgcc aaaccgtgcc gtgtgccgga
     7021 aggcgttcgg gcgcgccgtt cttccgtccg ggcggcgcct ggcagtgtgt caagttgcac
     7081 attgcgccgg ccgatcgggg caagggggcg tgacataaat taattcggtg gcaacataag
     7141 ccccgccatg tcgctcaacg tcgatcgctc gctgccaccc gatgccgcgc atccttcgcc
     7201 gccgcccggc ggcgggccgg tgccgtgtgc gctgttggag tcggccatgc taccggtagg
     7261 ggaccgcttc gacgtgtggc gcgaaagcgt gctgccgctg ttcgagtcca tccccgatga
     7321 ggcacccgat aatttccatg cgcgggtgga aagctatgac ctgcggcatc tggtgatggc
     7381 gatgagcgcg ttctcgccgt tgcgcttcca tcgtgaccgc catcatcgcg tggcggaagc
     7441 cgccgaccat ctgctggtgc agctttacgt ggagggcggt tacgtcggtc acaacggcta
     7501 tcgcgaggtg tgcgtgcggc cgggcgacat cagtctgctc gatttggggc gggaactgga
     7561 aacgcgcgcg ccggcgtcca acgcgctttc gctggtcatt ccgcgcgagc agttgtacgc
     7621 ctatgccagt ccggagcgcc tgactcacag cagcgtgttg cgcggcgatt cggtggtcgg
     7681 cagcgtgctc ggccatcatc tgctcaccgt ctgggggaag ctgccttcgg catgcgtcga
     7741 ggagatcgac gacatcaacc atctcctgct cagttccatc gtcggcgcct ttacgcccgc
     7801 accgaccgac ccgtccgggt tgccggagcg cattacgcag gaggcgatcc gcgcctacat
     7861 cgcgcaaaac cttaccgagc cgctcacgcc cgaactgctc tgtcatcgct ttgcctgttc
     7921 gcgcgcacag ctttatcggc tgttccagcc gttcggcggt gtggccgcct atatccgcga
     7981 cgcgcgtctc aaccgctgct ggcaggaact gaccgaacgc agcgatcggc cgaagcgcat
     8041 cggcgacgtg gcgatgcgtt ggggtttcac cagtcagagc catttcaacc gcctgttccg
     8101 ccaggccttc ggcgtgacgc ccggcgaggc cgtcgagcgt gcgcaatccc atgtaggcgc
     8161 cggatcgact gcgccccatg cggacactcg cccttctctg cgcgacttgt tgcgacgttt
     8221 gtaacgcgtt ttgccggtgc gagacaccgg gtcaaggata ttgagacgcg tggacaagcc
     8281 cgtcgcgggc acgctgccga taatgcgggc atggacaggc cgtctacgct cattgctgcg
     8341 acccggtgca acgctgcgct cgacgcgctg ctgaactgcg cggcggaagg tattttcggt
     8401 ctggaccggc tggggcgatg caccttctgc aatgcgtcgg cggcgcgcat gctcggttac
     8461 gacagtccgg cggaactggt gggccagcgc atgcacagtc tcatccatgc catgcgcgag
     8521 gacggcagcc cctgcgtcgc cgcgcaatgc gcgatgaatc gcgcttttct tgaacaccgc
     8581 agcgcgcatg acgacaccgc ctacatgtgg cgacgcggcg gcagcgcctt tccggtggaa
     8641 tattggaccg cgccgttgct cgaaaacggt acggtggtcg gcacggtggt gagcttcatc
     8701 gacatcaccg aacgcaaggc ctcggcgcac gcgctgcagc atcaggccac gcacgatgtg
     8761 cttaccggct tgcagaatcg cgcaacgctg cacggcgcga tccgcagcgc catcgaagcg
     8821 gccgacggcg aacgcggcat cgctatcgca ctgatcgacc tcgatcgctt caagcaggtc
     8881 aacgacacgc tgggtcattc ggtcggggac caggtgctgg tcgaactggc ggtgcgattg
     8941 cgtcaggcgg ccggcccgca gaacacgctg gtccgtctgg gcggcgacga gttcgccgtc
     9001 gtcatcggcg atgtcgcgca tgagcgggag gcgcaaaccg tcgcggagcg gttgctgaac
     9061 aaactgatat cgccggtcga ggtggcgggc atgagcctgc gtatcggcgc gagcatcggc
     9121 atcgctgttt atcccgagca cggcgaggat tcgagcgcac tgctgcgcaa ggccgatatc
     9181 gccatgtacg tcgccaaacg ccacggtgct gggtacaagt tctatcagcc cgagcgcgac
     9241 accggcgctt ccgcacggca tgccttgctg gtcgaactgc gcgacgcaat gagcgccgat
     9301 caattgcacc tgcgcttcca gccgcaactt tcgctggcga cgcggcaggc ggtcgccttc
     9361 gaagcgatgc cgcactggca gcatccgcag cgtgggctgc tgccgcctga ggaattcatg
     9421 ccgctggtcg gactgagcga tctggtgcag ccattggccc tgcgcatgat cgatcaggcg
     9481 ctcgcacagt gccggcagtg gcatcttgcg ggacacccgt tcaacgtggc gatacagatc
     9541 ccgtcgcgtt gcctgctcga tcagaccctg ccgcagcgca tcgaggcgct gttggccaaa
     9601 cacgaactcc cgccgcattt gctggaactg caaatgcgcg gtggtgccat ggccgatacc
     9661 cgacgcgcca tggagatcct gcacgtgctg ggcgggttgg gtatcggcgt cgtcatcgac
     9721 gacttcggca gcgcctacgg ctccctccgg gatctggcac ggttgcgtct gcgcggtgta
     9781 cgcatcgatt gcgccggtct gcatagcgac gacgaagcga aaatcatcgc gactgcagcg
     9841 gccatggcac acagcctcgg tgcctgtgtc attgccaagg gggtgcagaa ggagtgtgcg
     9901 ctcgccgatc tacagaaatt gggctgcgac cgcattcagg gcgagagcat cgcgccgccg
     9961 ctgacggcgg acgaaatcgg cccgtggttg cagcgaaacg gcggctgaac ggcgtacgcg
    10021 tctctgcagc ggcaactcga ttcaccgcgg tttgcagact ctgtcgcgaa ccgcatcccc
    10081 gcgagtgtgt tcacggctcg aggctttggt ataaacggcg cacttgattc gtgtcacgtc
    10141 gccgtgcaaa gcccgactgc cgaccccttg ctgctgctgt ccaccgacgc gctcgacccg
    10201 cacgtgcgct atgactattg gcgtgaaatg gtgttctgct acttcgacgc ggtgcccgat
    10261 gcaccggatg cggtgcagcg gttcgaaagt catgtggttt cgctgatcag cgagcgcggc
    10321 gaaatcaaca gctatcgcag ttccgatgtc accgggcagc gcacgcgccg gcaataccag
    10381 cgcgacggcg gcgacgaagt cgcgctgatc tacgtgctga aaggcaaggc cgtttatcaa
    10441 ccggagaacg gcgtgcccac cgaggctgca gccggcaagt tcttctgcta cgacagcgca
    10501 cgtcccgcgc gcgtgcaatg gagcgccggc gaacaattgc atttcagtct gccgcgtgcg
    10561 gcggtcatgg aagcgatcgg cggcgacgtg cccgagccgg atgttctcgc ccgcagcctg
    10621 aatgaatccc ggcttgcgcc ttatctgctg tcccagatcc gacggttgac gcgcgaggca
    10681 ccgcagctca gtcccttcga gcgcgcgacc atgctcgacg gcacgctgga actggcgaag
    10741 gcggtcctgc gcgggagcct gcatccggca cgagccttcg ccgacggcga gacggcgctc
    10801 tactactccg cctgcactca catcgacgcg catctcggca aaccggattt g
//
LOCUS       DATGOU010000002         9911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3557518, whole genome shotgun sequence.
ACCESSION   DATGOU010000002 DATGOU010000000
VERSION     DATGOU010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9911
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3557518"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..609)
                     /locus_tag="VKZ48_00055"
     CDS             complement(43..609)
                     /locus_tag="VKZ48_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76273.1"
                     /translation="MDRTITRVLSIVLALGVVLGVVWFVEQDERRLEEQGASHAETMR
                     AAVPQSAYRHDDAARVPAPVGGNQKVIYTCSNNGQTTISDRPCGNVVATHAARVAPEQ
                     ARVRSYREQYENLVAQRTPTPQPRPAPSALAEQEWERARQKERCERLHRRIASIDARA
                     RQRHSSQEADRLREQRRLLSNERAKVCR"
     gene            complement(952..3255)
                     /gene="metE"
                     /locus_tag="VKZ48_00060"
     CDS             complement(952..3255)
                     /gene="metE"
                     /locus_tag="VKZ48_00060"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HLU76274.1"
                     /translation="MAITHNLGFPRIGAQRELKFALEACWAGRSSAEELQAVGAQLRA
                     ENWSAQNGIDYVPVGDFSFYDHVLDTSFMLGNVPERVSALKGDPLDDYFRVARGRSAA
                     DGHHGCGVHAGEMTKWLDTNYHYIVPEFHAETRFSLDAAPLLAQLAEARAQGVKAKPV
                     LVGPVTYLWLGKAKDDSDKLALLQRLLPVYRELLQQLARQGVEWVQIDEPVLVTRLSQ
                     PWRNACATAYAALKGSGPKLLLTTYFGTLQDNLSLACELPVQGLHIDAVNARAEVDAV
                     VSRLAQNKVLSIGAINGRNVWKTDLNATLDWLEPLHAKLGDRLWLAPSCSLLHVPVDL
                     DTERKLDPEIRQWLAFARQKLEELKVIATALDRGREAVRQELDDNRAAVESRRASTRV
                     HNPAVKAALARLSPELARRKSPYAVRAPKQAALLKLPPYPTTTIGSFPQTAEIRQARR
                     RFRNGELDAEAYREAMQAEIARCVREQEALGLDVLVHGEAERNDMVEYFGEQLAGYVF
                     TESGWVQSYGSRCVKPPILYGDVHRPQPITVEWARYAQSLTDKPMKGMLTGPVTILNW
                     SFVRDDQPRSVSCYQLALALRQEVLDLEAAGIRVIQIDEAALREGLPLRRSEWAEYLQ
                     WAVESFRIAANGVRDEMQIHTHMCYSEFNDIIASIADMDADVITIETSRSHMELLEAF
                     EDFSYPNQIGPGVYDIHSPNIPSETHIVQLMHKAAERIPPERLWVNPDCGLKTRTWEE
                     VIPALRNMVAAAKALRAEHAKRTRRAA"
     gene            3353..4249
                     /locus_tag="VKZ48_00065"
     CDS             3353..4249
                     /locus_tag="VKZ48_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLU76275.1"
                     /translation="MLERIHLAIVTEVERLGSLTAAADALHLTQSALSHSIRKLERQL
                     GTEIWHREGRRLRPTQAGRYLLGVARRVLPQLEHAEAQIKEYAKGQRGTLRIGMECHP
                     CYQWLLKIVSPYLAKWPDVDVDVKQKFQFGGIGALFGHEIDLLVTPDPLRKAGLRFEP
                     VFDYEQVLVVGSDHPLKERDHVTPRQLADETLITYPVETDRLDIYNLFLTPAGIAPRR
                     HKTIETTDIMLQMVASGRGVAALPRWLVNEYATKLPITSIRLGRNGIAKQIHLGFREA
                     DAEVDYLKAFVELARKSRGRKR"
     gene            complement(4303..5136)
                     /locus_tag="VKZ48_00070"
     CDS             complement(4303..5136)
                     /locus_tag="VKZ48_00070"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLU76276.1"
                     /translation="MNLALDPQTAAPDYTAIKQRQQTVWSSGDYAMIGTRLQIVGELL
                     AEAVDLRADERVLDVAAGTGHMTLAAARRFAKVTSTDYVPELLDKARARVAADSLEAE
                     YRVADAEKLPFADGEFDVVVSTFGVMFAPDQARAASEMLRVLRPGGRIGLANWTPQGF
                     IGQLFKVQSSHVAPPPGVPSPVKWGDESFIEQCFGPHAAQIRCERRDFMYRFRSPQHW
                     LQVFRDYYGPTLKAFATLDESGKRALEKDILDLLSRMNIGDAQSLVVPAEYLEIVVTK
                     R"
     gene            5278..6336
                     /locus_tag="VKZ48_00075"
     CDS             5278..6336
                     /locus_tag="VKZ48_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLU76277.1"
                     /translation="MTGRPPELIEAPRPMDQDPLSDVLSSVRLRGAIFYYVSCSAPWA
                     IEAPPARMLADIVMPGADHVLAYHLIAKGDAWVEMDNAPSATLTEGDIIMFPRGDAHF
                     LSSARGLRAAPSDPQWVHATRNDPKPIPVAHYRGVLQPCAELPAEEADTVIVCGFIGC
                     DLRPFNPLITALPRQLHLSGNQVGAWVVPVLHQAVAESRSRRPGSAAVLERVSEMVFV
                     DAARRYLESMPDDASGWLAALRDRHVGRAIALMHERPAESWTIDELGRQVGLSRSALH
                     ERFVELVGQPPMQYLTSWRMQRGAQLLREGRATVATIAQEVGYDSEAAFARAFKRLTG
                     LPPAAWRRGQRFAALSAH"
     gene            6508..7116
                     /locus_tag="VKZ48_00080"
     CDS             6508..7116
                     /locus_tag="VKZ48_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HLU76278.1"
                     /translation="MKYTTIAAATVLALAGTAPAFAAGDYGSKSSSSQTQSMQGSTGA
                     TGSMQGQYDQQTIRQVQEKLSEKGHDVQADGQMGPKTQQALKKFQKDEGLRASGQLDE
                     QTLSALGVSESQSGSIGSQGSSGSDTGGLSGGSSGASGGGAQAGSSSSGSMGGSSGAS
                     GSMGGSSGASGSMSGSASDTGSATGSGGGMGGSSSGSAGGSR"
     gene            complement(7190..7456)
                     /locus_tag="VKZ48_00085"
     CDS             complement(7190..7456)
                     /locus_tag="VKZ48_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76279.1"
                     /translation="MALLFALIGVIAVAYAHANLAHFIAAPSGVHATRAVLIVVGAAF
                     GYVASAYVQHDALRWLMFVAGFGAVHVPPALVLLIKRARHSPKS"
     gene            7553..7735
                     /locus_tag="VKZ48_00090"
     CDS             7553..7735
                     /locus_tag="VKZ48_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76280.1"
                     /translation="MHTPQPEPDTPAPDVDPPPKPVPPDQEPDIVPEVDPRPVPPPVE
                     PPPDAPPPIRASSRHR"
     gene            7828..8295
                     /locus_tag="VKZ48_00095"
     CDS             7828..8295
                     /locus_tag="VKZ48_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HLU76281.1"
                     /translation="MKSRIAYFNKFPRAAQAMLGLQNYVNECGLEYSLVELVKMRASQ
                     INGCAYCLDMHSKDARAAGETEQRLYLLDAWREAPFYSDRERAALEWTEALTRLGVHG
                     VPDDIYNAVREHFSEEELVNLTLAVVAINGWNRLSIAFRPEVGSYQPAKAPKA"
     gene            8555..>9911
                     /locus_tag="VKZ48_00100"
     CDS             8555..>9911
                     /locus_tag="VKZ48_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003820458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLU76282.1"
                     /translation="MVTTTATLAVSSPREERKVIFASSLGTVFEWYDFYLYGSLAAII
                     SKQFFSGVNPTAAFIFALLAFAAGFAVRPFGAVFFGRIGDLVGRKYTFLITILIMGLS
                     TFLVGLLPSYATIGVAAPIILIGLRLLQGLALGGEYGGAATYVAEHAPHGKRGLHTSW
                     IQTTATLGLFMSLLVILGVRTYVGEEAFAAWGWRVPFLLSFILLIISVWIRLSLSESP
                     LYQEMKNQGKTSKAPLKESFGQWSNLKIVILALIGLTAGQAVVWYTGQFYALFFLTQT
                     LKVDAFTANILIAASLAIGTPFFIFFGWLSDKIGRKPIIMAGCLIAALTYFPLFKALT
                     HFANPALEQALSSSPVTVIADPKECSFQFNPVGTAKFTSSCDIAKSFLARNSVSYDNV
                     AAPPGTIAVVKIGEQEIPSIDGRLLDPESLKTRSAEVNDRLSAAIRAHGYPAKADPAQ
                     MN"
BASE COUNT         1816 a         3322 c         3062 g         1711 t
ORIGIN      
        1 gaaaccggcg gcggcttggg cgtgacggcg tcgaagcgaa gcttagcggc agactttggc
       61 gcgctcattc gagagcagcc tgcgctgctc ccgcaatcga tccgcctctt gactggaatg
      121 ccgctggcgg gctcgcgcgt cgatcgaagc aattcgccga tgcaatcgct cgcaccgttc
      181 cttctggcgt gctcgttccc actcctgctc tgctaaagcg gacggagcgg gtcgcggctg
      241 aggagtcggc gtccgctgcg cgacaagatt ttcgtactgc tcacgatagc tgcgcacacg
      301 cgcttgctcc ggcgcgacac gcgccgcgtg cgtcgcaacg acattgccgc aaggcctgtc
      361 cgaaatcgtg gtctgcccat tgttcgagca ggtatagata actttttggt tgcctccgac
      421 cggcgcaggc acacgcgccg catcgtcgtg gcgataagcc gactgcggca ctgccgcgcg
      481 catcgtttca gcgtgcgatg ccccctgctc ttccagacgc ctttcgtcct gctcaacgaa
      541 ccagacgacg ccaagcacaa cgcccagcgc cagaacaatg gagagtacac gtgtgatcgt
      601 ccgatccatc ggacaactct agatgccctg aggccaaagg accgagggca tccgtcacgc
      661 tgcggcgctc cgcacggaga cgcgatttac cacctgaaga agctttaatc accgaaagag
      721 aaggtccgtc gcatcgcgat cgtcattccg ggcacgcgta gccggcagtg ccggcgaagc
      781 acgatccgga atccacgacc gttcgtggtg agcgcccttc gactggctcg ggacaggctg
      841 cgcaatcgaa tcatgaacgg caagaaaccg gcggtggccg ttcacccttc gacgttgctc
      901 agggcgaacg gctcctgaat gcccgcctgc gcgggaatga cgacagcagc cctacgccgc
      961 gcgccgcgtc cgcttcgcgt gctccgcccg caacgccttc gccgccgcca ccatattgcg
     1021 cagcgcgggg attacctctt cccacgtccg ggtcttgagg ccgcaatccg ggttgaccca
     1081 cagacgctcc ggcggaatgc gttccgccgc cttgtgcatc aactgcacga tatgcgtctc
     1141 gctcggaatg ttgggcgaat ggatgtcgta cacgcccggt ccgatctggt tcggataact
     1201 gaagtcttcg aaggcctcca gcagttccat gtgcgaacgc gaagtctcga tggtgatcac
     1261 gtccgcatcc atgtcggcga tcgaggcgat gatgtcgttg aactccgagt agcacatgtg
     1321 cgtgtggatt tgcatctcgt cgcgcacgcc gttggccgcg atgcggaagc tctcgaccgc
     1381 ccactgcagg tactcggccc actccgagcg acgcagcggc agtccttcgc gcagcgccgc
     1441 ctcgtcgatc tggatgacgc ggatgcccgc cgcttccaga tcgagcactt cctgtcgcag
     1501 tgccagcgca agctggtagc aggacaccga gcgcggctgg tcgtcgcgca cgaaggacca
     1561 gttgaggatg gtcaccggcc cggtcagcat gcccttcatc ggcttgtcgg tcagcgactg
     1621 cgcatagcgc gcccactcca ccgtgatcgg ctgcggacga tgcacgtcgc catagagaat
     1681 cggcggcttc acgcagcgcg agccatagga ctgcacccag ccggattcgg tgaacacata
     1741 gccggccagt tgctcgccga aatactcgac catgtcgttg cgctcggcct cgccgtgcac
     1801 cagcacgtcc agtcccagcg cttcctgctc gcgcacgcag cgcgcgattt ccgcctgcat
     1861 cgcttcgcga tacgcttcgg catcgagttc gccgttgcgg aagcggcggc gcgcttgacg
     1921 gatttccgcg gtctgcggaa aggacccgat ggtcgtcgtc ggatacggcg gcagcttcaa
     1981 cagtgcagcc tgcttgggcg cgcgcacggc gtaaggactc ttgcgccgcg ccaactcggg
     2041 actcagtctg gcgagtgccg ccttaaccgc cgggttgtgc acgcgcgtcg aagcccggcg
     2101 gctttcaacg gccgcacggt tgtcgtccag ttcctgccgg acggcctccc gcccgcgatc
     2161 gagcgcggtg gcgatgactt tcaactcctc gagcttttgc cgcgcgaacg cgagccactg
     2221 acggatttcc ggatcgagtt tgcgttcggt atccaggtcc accggcacat gcagcagcga
     2281 gcacgacggc gccagccaca gacggtcgcc gagcttcgcg tgaaggggct cgagccagtc
     2341 gagcgtcgcg ttgagatccg tcttccagac gttgcggccg ttgatggcgc cgatcgacag
     2401 caccttgttc tgggccagtc tcgaaacgac ggcgtctacc tccgcgcgcg cattgacggc
     2461 gtcgatgtgc aagccctgca cgggcagttc gcacgccagc gagaggttat cctgcaaggt
     2521 gccgaaataa gtcgtcagca gtagttttgg gccgctgccc tttaatgcag cgtaggccgt
     2581 ggcgcaggca ttgcgccacg gctgcgaaag tctggtgacc aggaccggct cgtcgatctg
     2641 cacccactcc acgccctgcc gcgccagttg ctgcagcagc tcgcgataga cggggagcag
     2701 acgctgcaac agcgccagtt tgtcggagtc gtccttcgcc ttgccgagcc acagataggt
     2761 gaccggtccg accagcaccg gcttcgcctt cacgccctgt gcacgcgcct cggcaagctg
     2821 cgccagcagc ggcgcggcat cgagcgagaa ccgcgtctcc gcatggaact ccggcacgat
     2881 gtagtggtag ttcgtgtcga gccacttggt catttcgccg gcgtgtacgc cgcaaccgtg
     2941 gtggccgtca gcggcggatc ggccgcgggc cacgcgaaaa tagtcgtcca gcggatcgcc
     3001 cttcaacgcg ctgacgcgct cgggcacgtt gccgagcatg aagctcgtat cgagcacatg
     3061 gtcgtagaaa gagaagtcgc cgacgggcac gtagtcgatg ccgttttgcg cgctccagtt
     3121 ctcggcgcgc aattgcgcgc cgaccgcctg cagttcctcc gccgaggacc ggccggccca
     3181 gcaggcctcc agtgcgaact tcagttcccg ctgcgcaccg atgcgcggaa aacccagatt
     3241 gtgtgtgata gccatgggtc gctcccgaat tctcgaacgg ctcatggtag gcaaaccagc
     3301 tcatgaacaa aaatggtatt gtttcatcca ttcatgaatt cggatcatgc gcatgctcga
     3361 acgcattcat ctcgccatcg tcaccgaagt cgagcggctc ggctcgctga ccgctgcggc
     3421 cgacgcgctg catctcacgc agtccgcgct gagccattcg atccgcaaac tcgaacggca
     3481 gctcggcacc gagatctggc atcgcgaagg acggcggctg cgcccgacgc aggcgggacg
     3541 ctatctactc ggcgtggccc ggcgcgtgct gccgcaactg gagcatgccg aggcgcagat
     3601 caaggaatac gccaagggtc agcgcggtac gctgcgcatc ggcatggagt gccacccctg
     3661 ctaccagtgg ctgctgaaaa tcgtctcgcc ctatctggcg aagtggccgg acgtggacgt
     3721 ggacgtgaaa cagaaattcc agttcggcgg catcggcgcc ctgttcggcc acgagatcga
     3781 tctgctggtg acgcccgatc ctttacgcaa ggccggactg cgcttcgagc ccgtgttcga
     3841 ctacgagcag gtgctggtcg tcggctccga ccatccgctc aaggagcgcg accatgtcac
     3901 gccgcgacaa ctcgcggacg aaacactcat cacctatccc gtggaaaccg accggctgga
     3961 tatctacaac ctgttcctca cgccggcggg catcgcgccg cggcgacaca agacgatcga
     4021 gacgacggac atcatgctgc agatggtcgc gagcggccgc ggcgtggcgg cgctgccgcg
     4081 ctggctggtg aacgaatacg caaccaagct tcccatcact tcgatccggc tcggccgcaa
     4141 cggcatcgcc aagcagattc acctcggctt tcgcgaagcg gacgcggaag tggattacct
     4201 caaggccttc gtcgaactcg cgcgcaaatc gcgcggccgc aaacgttaag cagacacggc
     4261 gcgcagcgtt agtgctgcgc gccgtgccca ggtttgaacc actcagcgct tggtgacgac
     4321 gatctccaga tactccgccg gcacgacgag cgactgcgca tctccgatgt tcatgcgcga
     4381 caacagatcg aggatgtcct tttccagcgc acgcttgccg gattcatcga gcgtggcgaa
     4441 agccttgagc gtcggcccgt agtaatcgcg gaacacctgc agccagtgct gcggcgaacg
     4501 gaagcgatac atgaagtcgc gccgctcgca gcggatctgc gccgcatgcg gaccgaagca
     4561 ttgctcgatg aacgactcgt cgccccactt caccggcgag ggcacgccgg gcggcggcgc
     4621 tacgtgcgac gactgcacct tgaacaactg gccgatgaaa ccctgcggcg tccagttggc
     4681 cagaccgatg cggccgcccg gccgcagcac gcgcagcatc tccgacgccg cgcgcgcctg
     4741 gtcgggggcg aacatcacgc cgaaggtgga cacgaccaca tcgaactcgc cgtccgcaaa
     4801 gggcagcttc tcggcgtcgg caacgcggta ctccgcttcc agactgtcgg ccgcgacacg
     4861 cgcccgcgcc ttgtcgagca gttccggcac atagtcggtg gacgtcacct tggcgaagcg
     4921 gcgcgcggcg gccagtgtca tgtggccggt gcccgccgcc acatcgagca cgcgctcgtc
     4981 ggcgcgcagg tcgaccgctt cggcaagcag ttccccgacg atctgcagcc gggtaccgat
     5041 cattgcgtag tcgccgctgg accagacggt ctgctgacgc tgcttgatgg cggtgtagtc
     5101 gggcgctgcg gtctggggat cgagtgccag gttcatgctg ttctcctcat ggacggtggt
     5161 cggtttgcaa atgcgacaac gcgctcgaag cgccggcgca tggtgtaccg acgcacccgt
     5221 tgacgcttca ccggccgtcg tgcaaatttg ctcctccctc cgccgtcgcg acggtgcttg
     5281 accggccgtc cgccggagtt gatcgaggcg ccgcgcccga tggaccagga tccgctttcc
     5341 gacgtactga gcagcgtgcg attgcgcggc gcgatctttt actacgtcag ttgcagcgcg
     5401 ccctgggcga tcgaagcgcc gcctgcgcgg atgctggccg acatcgtcat gcccggcgcg
     5461 gaccacgtgc tggcctatca cctcatcgcc aagggcgatg cctgggttga aatggacaac
     5521 gcgccctcgg caacactgac cgaaggcgac atcatcatgt tcccgcgcgg cgatgcgcac
     5581 tttctgtcga gcgcgcgcgg tctgcgtgcc gcgccgagcg atccgcaatg ggtccatgcg
     5641 acgcgcaacg atcccaaacc cattcccgtc gcgcactatc gcggcgtgct gcaaccctgc
     5701 gcggagctgc cggccgagga agcggatacg gtgatcgtgt gcggcttcat cggctgcgac
     5761 ctgcgtccgt tcaatccgct catcactgcg ttgccgcgac aactgcatct gtcggggaat
     5821 caagtcggcg catgggtggt gcccgtgctg catcaggcgg tcgccgaatc gcgctcgcgc
     5881 cgcccgggca gcgcagcggt actggaacgc gtgagcgaga tggtgttcgt cgatgcggca
     5941 cgacgctatc tcgaatcgat gccggacgat gcatcgggct ggcttgccgc actgcgcgat
     6001 cgtcatgtcg gccgcgcgat cgcgctcatg cacgagcgtc ctgccgaatc ctggaccatc
     6061 gacgaactgg gcagacaagt cggtctttca cgttcggcac tgcacgaacg cttcgtcgaa
     6121 ctcgtgggtc agccgccgat gcaatatctc accagttggc gcatgcaacg cggcgcgcaa
     6181 cttttgcgcg aaggccgcgc gaccgtcgca accatcgcac aggaagtggg ctacgactcc
     6241 gaagcggcat tcgcgcgcgc cttcaaacga ctcaccggtc tgccgccggc cgcatggcga
     6301 cgcggccagc gcttcgctgc actctccgcg cactaagcgc gtctccatcg aacgacaccc
     6361 gttgttgtat cgggatgtaa gcgcaaccgc tttcgggcac gcctgctgcg tacaaagaaa
     6421 ggcatggacg gctgcatagt gcagcgtgat cagcgcacac cgcacgacgc tgtcctcctt
     6481 tgtctgacga accatcagga gatacacatg aaatacacga ccattgcggc ggccactgtt
     6541 cttgcgctcg caggtacggc gcccgcgttt gcagcaggcg actacggcag caagagttcg
     6601 agttcgcaaa cccaatccat gcagggcagc accggtgcaa cgggcagcat gcagggccag
     6661 tacgatcagc agaccattcg ccaggtgcag gagaagctga gcgagaaagg ccatgatgtg
     6721 caggcggacg gccagatggg gccgaagacc caacaggccc tgaagaagtt tcagaaggat
     6781 gaagggctgc gggcttccgg tcagctcgac gaacaaacgc tttccgcgct gggcgtcagc
     6841 gagtcgcagt ccggctccat cggcagccag ggcagctcgg gcagcgacac cggcggcctg
     6901 agcggcggca gcagcggcgc aagcggcggc ggcgcacaag cgggcagcag ctcgagcggc
     6961 agcatgggcg gcagctcagg tgcaagcggt agcatgggtg gcagctctgg cgcgagcggc
     7021 agcatgagcg gcagcgccag cgacaccggc agcgccaccg gctccggcgg cggcatgggc
     7081 ggcagcagca gcgggagcgc cggcggcagc cgttaatccc tcgctataaa gcaagtcgca
     7141 acgaagccgg gcgcgcaagc gccccggctt tttcttgcgt gcgaagaatt caggatttcg
     7201 gcgagtgccg agcgcgcttg atcagcagca ccaaagccgg cggcacatgc accgcaccga
     7261 agccggcgac gaacatcaac cagcgcaacg catcgtgctg cacataagcc gacgccacat
     7321 aaccgaaggc cgcgcccacc acgatcagca ctgcgcgcgt tgcatgcacg ccgctgggcg
     7381 cagcgatgaa atgcgcgagg ttcgcatggg cgtacgcgac ggcaatcacg ccgatcaacg
     7441 cgaatagaag cgccatgatc atgcatggca cgatgcaaca cgcattccga gcgcgagccc
     7501 cgagcaggcg agcgaagtgc tagaggcgaa ggtgtacgca caaggagctc acatgcatac
     7561 gccacaaccc gaaccggata cgcccgcccc cgacgtggac ccgccaccca agccggtgcc
     7621 gcccgatcag gagccggata tcgtgcccga agtcgatccg cgaccggtgc cgccgccggt
     7681 ggaaccgcca ccggatgcgc cgccgccgat cagggcatcg agccggcatc ggtaaagccc
     7741 acaccacggc ttcggtcgcc tcacacgagc caccgccgag acgagccggt cacgatcgca
     7801 gtatgctttg ctccgaaagg agccgacatg aaatcccgta ttgcatactt caacaagttc
     7861 ccccgcgccg cgcaggcgat gctgggactg cagaactacg tcaacgagtg cgggttggaa
     7921 tacagcctgg tcgaactcgt caaaatgcgt gcttcgcaga tcaacggctg cgcctactgt
     7981 ctcgacatgc acagcaagga tgcccgcgcc gcgggcgaga cggagcaacg gctttatctg
     8041 ctcgatgcct ggcgcgaagc gccgttctac agcgatcgcg aacgtgcggc gctggagtgg
     8101 accgaggcgc tgacccgact cggcgtacac ggtgtgccgg acgacatcta caacgccgta
     8161 cgcgaacact tcagcgaaga agaactggtc aacctcaccc tcgcggtcgt cgccatcaac
     8221 ggctggaacc gcctcagcat cgccttccgt cccgaggtcg gcagctacca gccggccaag
     8281 gcgccgaagg cctgaaccca cctgggcttg ttgcgccgtc gtcacacctt cccagggtgt
     8341 gccggcggcc cggcatgccc cgccgtttct gggccaggcc cctctctccc ctcctcctgc
     8401 atcgatggct cgcccgggcg caacctgtac ggacagccat gcctctaatt ttttcgttgt
     8461 gcgcgcgccc ggcccggatt tagcatggcg cacctcgaag cagccgccag aagactgtag
     8521 ggggtttcca ccaccttaga gacgaggagg cttcatggtt acgaccacgg cgacactggc
     8581 tgtaagctcc ccccgcgagg aacgcaaagt catctttgct tcctcgctag gcaccgtttt
     8641 cgagtggtac gacttttatc tctacggatc gctcgcagcg atcatctcca agcagttctt
     8701 ctccggcgtc aaccccaccg cagccttcat cttcgcgctg ctcgcattcg ccgccggctt
     8761 cgcagtccga cccttcggcg cagtcttctt cggtcgtatc ggcgacctgg tcggacgcaa
     8821 atacaccttc ctcatcacca tcctcatcat gggtctgtcc acgttcctcg tgggcctgct
     8881 gccctcctac gccaccatcg gcgtcgccgc gccgatcatc ctgatcggtc tgcgactgct
     8941 gcagggcctg gcgctcggcg gtgagtatgg cggtgcggcg acctatgtcg ccgaacatgc
     9001 gccgcacggc aaacgcggtc tgcacacctc atggattcaa accacggcga cgctcggtct
     9061 gttcatgtcg ctgctggtga tcctgggcgt gcgcacctat gtgggcgaag aagccttcgc
     9121 cgcctggggc tggcgcgttc cgttcctgct gtcgttcatc ctgctcatca tctcggtgtg
     9181 gatccggctg tcgctgtcgg agtcgccgct gtatcaggag atgaagaacc agggcaagac
     9241 ctccaaggcg ccgctcaagg agtccttcgg tcagtggtcg aacctcaaga tcgtgatcct
     9301 cgcgctgatc ggtctcaccg cggggcaggc ggtcgtctgg tacaccggcc agttctatgc
     9361 gctgttcttc ctgacgcaga cgctgaaggt cgatgcattc acggccaaca tcctgatcgc
     9421 agcctcactg gccatcggca cacccttctt cattttcttc gggtggttgt cggacaagat
     9481 cggccgcaag cccatcatca tggcgggctg cctgatcgcc gcattgacct acttcccgct
     9541 gttcaaggcg ctcacgcact tcgccaaccc cgcgctggaa caagccctgt ccagctcgcc
     9601 cgtcacggtg atcgccgacc cgaaggaatg ctccttccaa ttcaatccgg tcggcacggc
     9661 gaagttcacc agctcctgcg acatcgccaa gagcttcctc gcgcgcaact cggtgagcta
     9721 cgacaacgtc gcagcgccgc ccgggacgat cgcggtggtg aagatcggcg agcaggaaat
     9781 ccccagcatc gacggccgac tgctcgatcc cgaatcgctg aagacgcgct ccgcggaggt
     9841 caacgaccgg ctgagtgcag ccatccgcgc acacgggtat ccggcgaagg ccgatcccgc
     9901 gcagatgaac a
//
LOCUS       DATGOU010000003         2653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_1340452, whole genome shotgun sequence.
ACCESSION   DATGOU010000003 DATGOU010000000
VERSION     DATGOU010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340452"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="VKZ48_00105"
     CDS             complement(<1..835)
                     /locus_tag="VKZ48_00105"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HLU76283.1"
                     /translation="MKFSENWLRQHVSTTASHDELIATLTAIGLEVEEVTRMGDSLDG
                     VVVARIISAEKHPEADRLQVCQVDAGNGEIVQIVCGAPNARAGLNVPLAKVGAVLPGG
                     MEIKPAKLRGMESFGMLCSAKELGTDSDASGLLELPADATVGAPVTELLGLPDAAIEI
                     KLTPNRADCFGVRGIAFDVAAASDAEVSAFEIAPVAAQIDDALPIELTAGADAPRYCG
                     RVLRDVDATVRTPLWMAERLRRSGVRPVSLLVDVTQYVMLELGQPMHAFDRDLLTGPV
                     GV"
     gene            complement(852..1862)
                     /gene="pheS"
                     /locus_tag="VKZ48_00110"
     CDS             complement(852..1862)
                     /gene="pheS"
                     /locus_tag="VKZ48_00110"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HLU76284.1"
                     /translation="MSAIETLTAQALTDIAAADSPAAVESLRVALLGKSGSITAQLKQ
                     LGALPGDQRKAAGEAINRARNTVTEALGARKIELDAAALDVRLAAETIDVTLPGVNPS
                     RGGLHPVSRTLERISDIFGRLGFELADGPEIEDDWHNFEALNFPPHHPARAMHDTFYM
                     RPDGSGVARLLRTHTSGVQVRYMKDHAPPLRMIAAGKVYRSDSDQTHTPMFHQVEGLL
                     VDEHASFADLKGTLAEFVRAFFERDFQMRFRPSYFPFTEPSAEVDIAWEQPDGSMRWL
                     EVLGCGMVHPNVLRNIGIDPEKYTGYAFGLGVERFAMLRYGVDDLRSFFENDVRFLKQ
                     FA"
     gene            complement(1968..2327)
                     /gene="rplT"
                     /locus_tag="VKZ48_00115"
     CDS             complement(1968..2327)
                     /gene="rplT"
                     /locus_tag="VKZ48_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HLU76285.1"
                     /translation="MARVKRGVQARRRHKKVLDLAKGYYHARRKVYRVAHQAVIKAQQ
                     YAYIGRKQKKRNFRSLWITRINAAARMNGMSYSRFMNGLMKAGITLDRKVLADIAVHD
                     AVGFAALTEKAKGALAA"
     gene            complement(2341..2538)
                     /gene="rpmI"
                     /locus_tag="VKZ48_00120"
     CDS             complement(2341..2538)
                     /gene="rpmI"
                     /locus_tag="VKZ48_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HLU76286.1"
                     /translation="MPKIKTHRGAAKRFRKTASGKFKCGHANRSHILTKKATKRKRNL
                     RQTNHVRAEDAGRINRMLPYA"
BASE COUNT          451 a          859 c          849 g          494 t
ORIGIN      
        1 ggacgccgac cggaccggta agcaggtcgc gatcgaaggc gtgcatcggc tgacccagct
       61 cgagcatcac gtactgggtg acatccacca gcagcgacac cggccgcaca ccgctgcgac
      121 gcagccgctc ggccatccaa agcggcgtcc gcaccgttgc atcgacgtcg cgcagcacac
      181 gcccgcaata gcgcggcgca tctgcgccgg ctgtcagctc gatcggcagg gcatcgtcga
      241 tctgtgccgc gaccggcgcg atctcgaatg cggacacttc cgcatccgac gccgcagcga
      301 cgtcgaacgc gatgccacgc acgccgaagc aatcggcgcg gttcggcgtc aacttgatct
      361 cgatcgcagc atccggcagg ccgagcagtt cagtcaccgg tgcgcccacg gtggcgtcgg
      421 cgggcagctc caacaggccg gaggcgtcgc tgtcggttcc cagctccttg gccgagcaca
      481 acatgccgaa cgactccatg ccgcgcagct tggccggctt gatctccatc ccgccgggca
      541 acaccgcccc gactttcgcc aacggcacgt tcaggccggc gcgcgcgttg ggcgcaccac
      601 agacgatctg cacgatctcg ccgttgccgg catccacctg gcacacctgc aggcggtcgg
      661 cctccgggtg cttctcggcg gaaatgatcc gggcaacgac gacgccgtcg aggctgtcgc
      721 ccatgcgggt gacttcctcc acctcaagtc cgatcgcggt gagggtggcg atcagctcat
      781 cgtggctggc cgtggtggat acgtgctggc gcagccagtt ttcggaaaac ttcattcaat
      841 cgatccccgg cttacgcgaa ctgtttcagg aaacgcacat cgttctcgaa gaaactgcgc
      901 aggtcatcga ccccgtagcg gagcatggcg aaacgctcca caccaaggcc gaaggcgtat
      961 ccggtgtact tctccgggtc gataccgata ttgcgcagca cgttggggtg gaccatgccg
     1021 cagcccagta cctccagcca acgcatgctg ccatcgggct gttcccaggc gatgtcgacc
     1081 tcggccgacg gctcggtaaa cgggaaatag ctgggccgga aacgcatctg gaagtcgcgc
     1141 tcgaagaagg cgcgtacgaa ctcggcaagc gtgcccttga gatcggcgaa actggcgtgt
     1201 tcgtccacca gcaggccttc gacctgatgg aacatcggcg tatgggtctg gtcgctgtcg
     1261 ctgcggtaga ccttgccggc ggcgatcatg cgcagcggcg gcgcgtgatc cttcatgtaa
     1321 cggacctgca cgcccgaggt atgggtgcgc agcagacgcg cgacgccact gccatcaggg
     1381 cgcatgtaga acgtgtcgtg catcgcccgc gccggatggt gcgggggaaa gttcagcgct
     1441 tcgaagttgt gccagtcgtc ctcgatttcc ggcccgtcgg ccagctcgaa gcccagacgg
     1501 ccaaagatat cgctgatccg ctccagcgtc cggctgaccg gatgcaggcc gccgcgggac
     1561 ggattgacgc ccggcagggt cacatcgatg gtttcagccg ccaacctcac gtccagcgcc
     1621 gcggcatcca gctcgatctt gcgcgcgccc aacgcctcgg taacggtgtt gcgagcccgg
     1681 ttgatcgcct cgccggcggc cttgcgctga tcgcccggca aggcgcccaa ctgcttgagc
     1741 tgggcggtga tgctgccgga tttgccgagc agtgccacgc ggagggattc caccgcagcg
     1801 ggcgaatcgg cggctgcgat atccgtcagc gcctgggcgg tcagggtttc gattgcgctc
     1861 attctggtcc cataaaaaaa catggggaag gacttgcgcc cttccccatg cgtgtatctc
     1921 gttgctgacc ggagcgcggg tatggacccg cgcgccgatg cggcacctta cgctgcgagc
     1981 gcgcccttgg ccttctcggt caacgcggca aaacccaccg cgtcgtgcac ggcgatgtcc
     2041 gccagcacct tgcggtcgag ggtgatgccg gccttcatca ggccattcat gaagcggctg
     2101 tagctcatgc cgttcatgcg ggcagccgcg ttgatgcggg tgatccacag cgagcggaaa
     2161 ttgcgcttct tctgcttgcg gccgatgtag gcgtactgct gtgccttgat gaccgcctgg
     2221 tgggcgacgc ggtagacctt gcgacgggcg tggtagtagc ccttggcgag atccaatacc
     2281 ttcttgtggc gacggcgcgc ctgtacacca cgtttgactc gtgccatttc tcagttctcc
     2341 tcaggcgtac ggaagcatgc gattgatacg gccggcatcc tctgcgcgga cgtggttcgt
     2401 ctgccgcagg ttgcgcttcc gcttggtcgc cttcttggtg aggatgtggc tacggttggc
     2461 gtggccgcac ttgaacttgc cggaagcggt cttgcgaaaa cgcttggccg ctccacggtg
     2521 ggtcttgatc ttgggcattg cgaaggtcct ttcgggtatt tgtcactgac atgggcggtg
     2581 gcatggccac actttccgtc ctgcccttgc cggtcgataa gtcattgatc cgaaaaggat
     2641 cagcaacagg tcc
//
LOCUS       DATGOU010000004        21584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_454578, whole genome shotgun sequence.
ACCESSION   DATGOU010000004 DATGOU010000000
VERSION     DATGOU010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 21584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21584
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454578"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            577..1572
                     /locus_tag="VKZ48_00125"
     CDS             577..1572
                     /locus_tag="VKZ48_00125"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HLU76287.1"
                     /translation="MSDQTDNIRPPHAPESSWAQRLLGDFHVTGLFWYRLHRWAVEKV
                     PEPLLAPLVGFFTSCFFCFIHKIRNAIGANLDVVLGPAGWLKRQRRIWRTMYSFAWCL
                     TERYERLVTDRTFQVECEAMEHWHAVANSGRGFVMVTAHLGLYEVGSMLPAGTETPHV
                     HLVREPEIDPRAQAFIEESVAAVESAHYTMHFQTGDPLFALTLAQALERGEVVAIQGD
                     RPRTGGKTVQTELFGRPFTLPVGPAVLSRAAGVPMIPVFAIREGRRRFRLVFRPPIET
                     PRTRNRDEDLRAATTQLARELEYGIRRAPYQWFVFRALWRNGSIGAGEKGTDLSA"
     gene            complement(1531..2280)
                     /locus_tag="VKZ48_00130"
     CDS             complement(1531..2280)
                     /locus_tag="VKZ48_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76288.1"
                     /translation="MDLTPSAAYFAGHFPAQPILPGITQIALAVAAMRREAEVPTLRA
                     VRHARLRRLVAPGDRLALRRRPASDALIRFELRVADRVVTNGEVEFGPLVSWEGRLPA
                     FGPVVDHPPIEQLLPHRPPMLFVRRVLAQGAEMIVCEAELPPDCPLADESVAPAITAV
                     EAAAQAAAVWEGLRRRDAAEASSGSRMGYLVSLRDVVFTQASLPVKRPFQVVVRLESR
                     MPPLSTYAVEAYCDGLPLLRGEIGTFLAGAD"
     gene            complement(2282..3460)
                     /locus_tag="VKZ48_00135"
     CDS             complement(2282..3460)
                     /locus_tag="VKZ48_00135"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLU76289.1"
                     /translation="MADDDHIEALQAMVPASVRALLGETFIASCGLIAQYTTCLATRV
                     FEELGLARLCDGQFRSVASLVSSANLMSQADVPVKWLMETLAANGLVERRVDADGTPE
                     FRASSDWAPMDSRPLLHEQERLDNRCLAAYTIAALAAERYPAVLRGEVTGETALFGPD
                     TFDAWCAYFSNDNPLYAISNAIGAVVVAEQLTGEGNALLEMGGGLGSGAQAVWERLAQ
                     EGRTNCVASYRFTEISGMFLRRAQKSLRPAFPEAPFSFAKLDIDRPFAEADVEPASCT
                     LIYGVNVLHVAHDLAATLSEIRNALAANGTLVISECVRPFADQPIYVEFVFNLLGAFR
                     APVLVPAWRPNGGFLTPEQWRAALEANGFKNVRFLPDVTRIRDRIPAFVAAGIAAERA
                     "
     gene            complement(3614..4417)
                     /locus_tag="VKZ48_00140"
     CDS             complement(3614..4417)
                     /locus_tag="VKZ48_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLU76290.1"
                     /translation="MPEQNCDAHRFADYIAVIPAYNEVRTVRNVVERTLAQGIRVIVV
                     DDGSTDGTADALRDMPIRLLRNPENLGKAASLWRGIQAAKADGARAVVTLDADDQHFP
                     EDIPRLIQRHEALPDHIVIGSRLHAREKIPVARYRANRFANFWIAWAAGYPLRDSQSG
                     FRIYPLALFDRVRVRHDRASCFVFESEILIEAGRQGIYSVAVPIAVSYGKHHRGSYFR
                     PVLDIARVARMLAVKLIGRGMYLPGLIRSLRRRSDDAVTSAAGESRQQR"
     gene            complement(4410..5132)
                     /gene="fabG"
                     /locus_tag="VKZ48_00145"
     CDS             complement(4410..5132)
                     /gene="fabG"
                     /locus_tag="VKZ48_00145"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HLU76291.1"
                     /translation="MKKALVSGGSGALGEAISRTLARDGLHVVVHAHAHPARAERIVE
                     TIRAEGGSAEAICFDVRDHQVCGDALEALVERIGPIQVIVNNAGIHDDVPLAGMSEAQ
                     WHNVVSVSLDGFFNVTRPLLLHMLSTRWGRIVNLSSVSGLIGNRGQVNYAAAKAGLHG
                     ATKALALEVASRGVTVNAVAPGVIASPGTDAAVSDAAVRSMVPMKRRGTPGEVAALIG
                     FLASDQAGYITGQIISVSGGLA"
     gene            complement(5143..5940)
                     /locus_tag="VKZ48_00150"
     CDS             complement(5143..5940)
                     /locus_tag="VKZ48_00150"
                     /inference="COORDINATES: protein motif:HMM:NF025100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HLU76292.1"
                     /translation="MRVQVRIQGVGVLAPGMPDWPTARSVLRGETAFDPTIVPKPDAA
                     ILPPAERRRASAAIRTSLSVALQAIRNAGVEASEVATVFATSDCDAENLNHLCEALAA
                     PQPEISPTRFHNAVQNAPAGYWTIAGACTQASSTVNSYDWVIAHGLLEAAVMAVAESR
                     TVMFVAHDLPMPPPLYALRPIESGFAVALVLAPDPAASGALLDLRFGAREGGEATRMA
                     HPQLEVLRASVPSAQALPLLESLALPRAGDVAIDYGDGRWLDVTVRV"
     gene            complement(5937..7127)
                     /locus_tag="VKZ48_00155"
     CDS             complement(5937..7127)
                     /locus_tag="VKZ48_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006491657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HLU76293.1"
                     /translation="MPPLHLNALTFVSALGAGLAATRDGLSQDRSGLRRNDFEYAPLD
                     CYIGRVDGLETVRLPAALQSYDCRNHRLALVALEQDGFAQAVARARARHGADRVAVLL
                     GTSTSGILSTELAYQRRDPETGALPNDLRYDHTHNFGALAEFVRLYLQLAGPSLAIST
                     ACSSSAKVVASAQRLIDTGICDAAVVGGVDSLCRTTLYGFNALQLVSREPCKPFDATR
                     DGLSIGEAAGFALLERDPDEASDGIAVRGYGESSDAHHMSAPHPEGRGAQAAMRQALS
                     RAGLTADAIDFVHAHGTATRNNDGTESIAIHDVLGAATPVASTKGAFGHTLGAAGIAG
                     VVVSCLALKHAFVPGTVNTSAVDPECAAHIQLCSEPRRLRNVVTNAFGFGGNNCSLVI
                     GLPS"
     gene            complement(7184..9499)
                     /locus_tag="VKZ48_00160"
     CDS             complement(7184..9499)
                     /locus_tag="VKZ48_00160"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HLU76294.1"
                     /translation="MIFRRPIVLLWCVLLALAVWIVFARTTISADLVAFLPRSATPKQ
                     QLMVDQLRDGVASRLVLFGIEGGDTPRLAAASRAFAKALQQTPHFSYVNNGEQQLGQR
                     DRQFLFEQRYLLSHQVVPERFSVDGLRAALEAALGRLASSGGLFYQASLARDPTGEIF
                     AVIQPLMDGSGPATREGVWFDDAGRRALLVAETHAAGFDMDAQARVQADIRAAFERVR
                     GEDDLRLLYTGPSVFAVQSRAAIESDAWRLSMLATLFVATILLLAYRSLPLLGLGLLP
                     VATGVLTGIAAVSLGFGDVHGITLGFGATLIGEAVDYPTYVFTQIAPGETVRDAARRV
                     WPTLRLAVLTTVFSALAMLLSSFQGLAQLGLFSLSGVLAAGLVTRYVLPQLVSAPLRS
                     VRALPPLHAGRIALAGWLRWTVIGALIVSAAFLATRGPQLWENDLANLNPIPQAARDL
                     DQRMREALGAPDVRLLYAVQAPTRDEALRGAERLASRLDELKQRGLIGSYDSPAFYLP
                     SLAAQQARQAALPDERTLRSRLAEAATGLPFQSGLFEPFISDVQQARNAPPLTPQSLA
                     GTGLELKLKALLSESGDRWLALLPLAGVKDAQSVADASAAFTDESVFLLDLKAESNRM
                     VRDYRNESLGLWCIGALAIAAVLWAGLRRGRAVLSVMSPVVCAVFVTAALLAAFGARL
                     NLFHLVSLLLVLGVGMNYALFFNRNLRDDHERARNVFALLVCALTTTSAFGALAFAAS
                     PVLQSIGITVGTGAVLSLLFSALWRPAGRSG"
     gene            complement(9496..10080)
                     /locus_tag="VKZ48_00165"
     CDS             complement(9496..10080)
                     /locus_tag="VKZ48_00165"
                     /inference="COORDINATES: protein motif:HMM:NF039988.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-related protein"
                     /protein_id="HLU76295.1"
                     /translation="MVRMIERSLCVLLWLALSWAAPASGAWNVESLMDALGRTTQSEA
                     RFTETKHLAALTIPLELRGTLRFQAPDHLERLVEAPFEERFIVDGDRVTFERKVTGER
                     HQLSLQSQPVLWAFIESIRATLRGDLATLQKFYRVELDGAQHSWTMSLLPREVEMGQF
                     VSVIRISGSDGNLHTIDVQEANGNRSITRIEASR"
     gene            complement(10068..11396)
                     /locus_tag="VKZ48_00170"
     CDS             complement(10068..11396)
                     /locus_tag="VKZ48_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLU76296.1"
                     /translation="MNALPLIAHENLSRPVAWHPSGPISAARYLREVRDLAGRLPPAR
                     YVLNLCDDRYRFMVVFGAALVNGQTCVLPPNHAQRVLGQVRAACVDHVCVVDHAEAGH
                     ADAHAYPDDLGVRSNDDAVPLIAPDHVAALVFTSGSTGDPRPNAKAWGRLVAGAHSER
                     EALGLDAAQRYAYVGTVPPQHMYGFESTVLLPMQTGGAVHASRPLFARDVATCLAQVP
                     APRVLITTPIHIRALLEEGPSLPPLARAVSAAAPLAREPAEDFERRYGCELREIYGFT
                     EAGMLATRRTSTEDAWRLMPGIRLERDGDGWCALGGHVNGRPPFSDVIEMQGEAGFSL
                     VGRAQDMVNIAGKRASLGDLNHHLNRIPGVRDGVFHLPRGDGRRVQRLMAFVVAPGMS
                     EADIMAALRPVIDPAFLPRPLIKVDALPRTASGKLPLDRLRELESMAWSG"
     gene            complement(11399..12007)
                     /locus_tag="VKZ48_00175"
     CDS             complement(11399..12007)
                     /locus_tag="VKZ48_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76297.1"
                     /translation="MRPVALALGVCSPLLLHLALLSDRPALAAACTLLVGLLVLWKRV
                     ATVLRWPLLGLALLICAAAALSDAAALGMVYVAPVVIYLCVGMLFGRTLVGEREPLVA
                     RIARLDRGDVLPDDLARHARGVTWAWTLLMAFLILTSVALALFGTPHAWSWFNNVWTF
                     VLIGGLFLGEYAYRLLRFRHYPHNSPLHVARLMVRNAPELLR"
     gene            complement(12021..12287)
                     /locus_tag="VKZ48_00180"
     CDS             complement(12021..12287)
                     /locus_tag="VKZ48_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HLU76298.1"
                     /translation="MSTKTPLEAELSALMVEALNLEMTPEEIDPQVGLYGDGLGLDSI
                     DLLELSLAISKKYGIQLRADDADNQRIFASVASLAQHIEANRTK"
     gene            complement(12344..13675)
                     /locus_tag="VKZ48_00185"
     CDS             complement(12344..13675)
                     /locus_tag="VKZ48_00185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLU76299.1"
                     /translation="MEDSYDYDVIVIGGGPGGSTAGALLAERGWCVAVLEKDRHPRFH
                     IGESLLPQNNALFERLGVLEEVERISMIKRGAQFSSMYHNKEQTFYFANALDKDWPYG
                     FEVHRSELDQLLFENARRRGARMYEETRVVRAEFDAAGVTVGIRTDAGERDLRARFLI
                     DASGRDTFLGNKLKLKQAHRKHASAAIYGHYRNAQRLPGEDAGNITIYWFDHGWFWFI
                     PQTHDITSVGAVCWPYYLKQRKGSLEDFLDATVAMVPALAERLRDAQRIAPVTATGNY
                     SYECRRAIGARHLMVGDAFAFVDPVFSTGVYLAMSSAFRAADTVEECLRRPEKAAAAL
                     TQYERGLRRSIATFSWFIFRITTPAMRELLLNPRNVLRVEEAMVSLLAGDIYRANGVR
                     WRIGFFKLVYYLTSLRRPRMAWAAARRRRQIIRPVGLQTDAASMEAGGSKG"
     gene            complement(13698..14531)
                     /locus_tag="VKZ48_00190"
     CDS             complement(13698..14531)
                     /locus_tag="VKZ48_00190"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HLU76300.1"
                     /translation="MRLLRLLCAASGLLLSAPVLALDEYPLWEAGVGIGALSLPDYRG
                     ADERTHYVFPLPYFIYRGEFLRVDREGAQTLLYRGPRAKFDLSLSAANPAKSDENRAR
                     EGMRDLNPTFEVGPRFTYRLAGAPHADYELTFQLPVRTVAEVDWPHVRHVGWLTNPLL
                     NLDIRNVAGTAWNMGIQTGPVFATRSYHEYYYGVDPQYARAGRPAYEADGGYSGTQVS
                     VGGSRRYKKWWMGAFVRYYDLHGSAMADSPLVKRKNSLFAGLGFAYIFAESSRKVLAD
                     R"
     gene            complement(14653..15852)
                     /gene="tyrS"
                     /locus_tag="VKZ48_00195"
     CDS             complement(14653..15852)
                     /gene="tyrS"
                     /locus_tag="VKZ48_00195"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HLU76301.1"
                     /translation="MSDIEQQLTVLKRGAEELLVEQELRDKLKTGRPLRVKLGMDPTA
                     PDLHLGHTVVINKLRHFQDFGHTVLFLIGDFTGMIGDPSGRNATRPPLTREQVETNAQ
                     TYRDQVFKILDPARTEIVFNSTWCNALGSAGLIKLASSYTVARMLERDDFAKRFRANQ
                     PIAVHEFLYPLIQGYDSVALKADVELGGTDQKFNLLVGRELQKDYGQPQQCVLTMPLL
                     EGLDGVNKMSKSLGNYIGITEAPAEQFGKLMSVSDTLMWRYIELLSFASLETVDKWKA
                     EVEQGRNPRDIKVLFAKEIVTRFHDAAAADAAERDFEARFRYHAAPENMPEVELHAQD
                     DSMPIAQVLKQAGLTASTSEALRMIDQGGVKLDGERVSDRSLALARGTNAVLQVGKRK
                     FARVVVR"
     gene            15912..17225
                     /locus_tag="VKZ48_00200"
     CDS             15912..17225
                     /locus_tag="VKZ48_00200"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HLU76302.1"
                     /translation="MRKNKFSILSQQDAARDRKKWRVGIVGIALLFGGMTAFGTVQQS
                     DEPEINPETVIEELALSFGESLIDDGDQVYWREERFARGDTFMSFLSRLGVNSEDART
                     IVRQNGGAQFFRTIGPGMSAQAETNAQGDLYTLRLLSGRDSVVGLKREGDRFTEFDEA
                     IPLTRTVQMSAGAISSSLFGSTDAAGLPDAIATQIAEIFSGDVDFHRDLRRGDRFSVV
                     YEMYYHGGQAVKAGRVLAAEFVNNGRTFRAAWYEDKDGRGSYYAPDGKSLRKAFLRSP
                     LEFSRVTSGFSRRFHPILKQWRQHKGVDYGAPTGTRIRATADGVVDFVGRKGGYGNTV
                     VLRHHAGITTLYAHMNGFAKGIRRGARVEQGQVIGYVGATGWATGPHLHYEFHVHGVH
                     RNPLTIAMPSADPLPARKIREFKEQVQPVLAQLDFLSDVRVASID"
     gene            17235..18326
                     /locus_tag="VKZ48_00205"
     CDS             17235..18326
                     /locus_tag="VKZ48_00205"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HLU76303.1"
                     /translation="MSALYIGLMSGTSLDGVDAALVDFAGDRPSVRGTRFLPYPDSIR
                     AEALALNSPGDDEIHRSALLSRELSRLYAEAVELLLGECGVMPRAVAAIGCHGQTVRH
                     RADLGYTVQLVDPALLVERTGIPVVANFRARDLAAEGQGAPLVPAFHAGAFRHADRDR
                     VILNVGGIANITALPASGTIIGFDTGPGNVLLDMWCARQRGCAFDADGEWAASGRVVE
                     ALLDDMLADPYFALPPPKSTGRDLYNEAWLMRRDLARYAAEDVQATLLELTARSVADA
                     IERYCPGADELYVCGGGASNRQLLRRLQALLGGVSVSTTADLGLHPDWVEAVAFAWLA
                     RRTLLQQPGNLPEVTGAQGPRVLGAVYPT"
     gene            complement(18395..18745)
                     /gene="erpA"
                     /locus_tag="VKZ48_00210"
     CDS             complement(18395..18745)
                     /gene="erpA"
                     /locus_tag="VKZ48_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HLU76304.1"
                     /translation="MNTATDMPAPLIFTDSAASKVKQLIDEEGNSELKLRVFVSGGGC
                     SGFQYGFTFDEVANEDDTVMEKNGVSLLIDPMSYQYLVGAEIDYTEGLEGAQFVIKNP
                     NAQTTCGCGSSFSA"
     gene            complement(18879..19907)
                     /gene="argC"
                     /locus_tag="VKZ48_00215"
     CDS             complement(18879..19907)
                     /gene="argC"
                     /locus_tag="VKZ48_00215"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HLU76305.1"
                     /translation="MIKVGIVGGTGYTGVELLRLLAQHPEVELKAITSRKDAGLPVTD
                     MFPSLRGRVSLAFSEPANAGLEKCDVVFYATPNGVAMQEVRTLLDAGVRVIDLAADFR
                     LKDIAEWEKWYKLPHAAPELLAEAVYGLPEVNREQIRNARLIANPGCYPTATQLGFLP
                     LVEAGVIDLDHLIADVKSGVSGAGRKAETHLLFAEAGDNFSAYGVAGHRHLPEVRQGL
                     ARAAGKPVGLTFVPHLTPMIRGIHATLYARITKEADFQKLYEDRYRDEPFVDVLPPGR
                     HPDTRSVRAANMCRMAVHRPQGGDTLVVLSVIDNLVKGAAGQAVQNLNIMCGFEERTG
                     LDQLPVLP"
     gene            complement(19999..20391)
                     /gene="rpsI"
                     /locus_tag="VKZ48_00220"
     CDS             complement(19999..20391)
                     /gene="rpsI"
                     /locus_tag="VKZ48_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HLU76306.1"
                     /translation="MVGEYYYGTGRRKSAVARVFIKPGKGQFIVNGKPVDDYFGRETG
                     RMVVRQPLELIDALNGFDIKVNVHGGGEFGQAGAVRHGITRALIEYDAELKPTLSNAG
                     LVTRDAREVERKKVGFHKARRRKQFSKR"
     gene            complement(20391..20822)
                     /gene="rplM"
                     /locus_tag="VKZ48_00225"
     CDS             complement(20391..20822)
                     /gene="rplM"
                     /locus_tag="VKZ48_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HLU76307.1"
                     /translation="MKTFSAKPHEVRRDWFVVDASDKVLGRLAAQIALRLRGKHKPEY
                     TPHVDTGDYIVVVNAAKLRVTGNKALQKKYYRHTGYPGGIYETTFEKLHGRYPARVLE
                     KAVKGMLPKGPLGYAMAKKLKVYADDVHPHSAQQPQNLELN"
     gene            21162..21584
                     /locus_tag="VKZ48_00230"
     CDS             21162..21584
                     /locus_tag="VKZ48_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HLU76308.1"
                     /translation="MECTVKWVDGMSFVAETGSGHILNMDGAPEGGGRNLAPRPMETV
                     LAGAGGCTTYDVVLILKRSNQDVRGCEVRVDAERAETDPKVFTKIHMHFIIRGRNLRP
                     ALVESAIKLSHTKYCSATLMLGKTAEITHDYEIIDDAL"
BASE COUNT         4051 a         7113 c         6774 g         3646 t
ORIGIN      
        1 tcttcagttc aatctctgtt tcaatatcgc taagcgattt cgcgctaccc accgaagcgg
       61 ataacactac tcatctcaaa agaaccaaca aagagaagat gaaatccttg actccacttc
      121 cacttcgtca atccttcaaa gcatgagcac caagtaatgt tcgagataac caccaacacc
      181 taagcatcag cggcccgtac accaacacca cccagcaccc acacctatcg gctgttcagt
      241 ttttaaagag cggtttcctc gttcgctgcg atgcgctgca tcgaggaaag ccgcgcatta
      301 tatagggaga aaaacttccg tcaacacttt gcacaaaaca tttgcgtgcg cgtgatgcga
      361 atgcattttt aaacgccgtt accgcactgc aaaattcgcg cggcgcggat gttcaaccgg
      421 gtgttgcgac agttattcga acggaaaaaa gcgtctgcac tgttatcgat ttcgcaagac
      481 ggcgcgcgct tctgtcgtgc acaagcgctc cgcaccgcct tggagtcgga gcggatcgtg
      541 cggcacaatg ccgcccgcct cgtaccgccc tttcgcatgt ccgatcaaac cgacaatatc
      601 cgtccgcctc acgcacccga atcgagctgg gcgcagcgcc tgctgggcga tttccacgtc
      661 accggcctgt tctggtaccg gctgcatcgc tgggcagtcg agaaggtgcc ggagccgctg
      721 ctggcgcctc tggtcggctt tttcacctcc tgcttttttt gcttcatcca caagattcgc
      781 aacgccatcg gcgcgaatct cgacgtcgtg ctcggcccgg caggttggct aaagcgccag
      841 cggcgcatat ggcggacgat gtacagcttc gcctggtgcc tgaccgagcg ctacgagcgg
      901 ctggtgacgg accggacttt ccaggtcgag tgcgaggcga tggagcactg gcacgccgtg
      961 gcgaacagcg ggcgcggctt tgtcatggtg accgcccatc tggggctcta cgaagtcggc
     1021 tcgatgctcc ccgccggcac ggaaacgccg catgttcacc tggtgcgcga gccggaaata
     1081 gacccgcgcg cccaagcctt catcgaggag agcgtcgcag cggtggagag cgcgcactac
     1141 accatgcatt tccagaccgg cgatccgctt tttgcgctga cactcgcgca agcactggag
     1201 cgcggggagg tcgtggccat ccagggcgac cggccgcgca ccggcggcaa aacggtacag
     1261 accgagttgt tcggacggcc gtttacgttg cctgtcggtc ccgcggtgct atcgcgcgcg
     1321 gcgggcgtcc cgatgatccc ggtgttcgcc atccgggaag ggcgccgccg tttccgtctg
     1381 gtgttccgcc cgccgatcga aacgccgcgc acacgcaatc gcgacgagga tctgcgtgcg
     1441 gccaccacgc agcttgcgcg cgaactggag tacggcatcc gccgcgcacc ctatcagtgg
     1501 ttcgtgttcc gcgcgctgtg gcgcaacggt tcaatcggcg ccggcgagaa aggtaccgat
     1561 ctctccgcgt agcaacggca gtccgtcgca ataggcttcc acggcgtagg tgctcaacgg
     1621 cggcatccgc gattccagac gcacgacaac ctgaaacgga cgcttcaccg gcagcgaagc
     1681 ctgtgtgaac acgacgtcgc gcaggctgac cagatagccc atgcggctgc cggacgacgc
     1741 ttcagccgcg tcgcgccggc gcagtccttc ccagaccgcg gccgcctgcg ccgcggcttc
     1801 cacggctgtg attgcgggtg ccacactctc atcggccaaa gggcaatcgg gaggcagttc
     1861 cgcctcgcag acaatcatct ccgcgccctg cgccagcaca cgccgcacga acagcatcgg
     1921 cggccggtgc ggcagcaatt gctcgatggg cgggtgatcc acaaccggcc cgaacgccgg
     1981 caagcggccc tcccaggaca ccaacggccc gaattccacc tcgccgttgg tgacgacgcg
     2041 atccgccaca cgcagttcga agcggatcag cgcatcgctt gccggacgcc ggcgcaacgc
     2101 aaggcgatcg ccgggtgcga ccagccggcg cagtcgcgca tgccgcacgg cgcgcaacgt
     2161 cggcacctcc gcctcgcggc gcatcgccgc caccgcaagc gcgatttgcg tgatgccggg
     2221 aaggatcggc tgtgccggaa aatgccccgc gaaatacgcg gccgacggcg tcaaatccat
     2281 gtcaggcccg ttcggctgcg ataccggctg cgacgaaggc aggaatgcga tcgcgaatgc
     2341 gcgtcacatc cggcaggaag cgcacgtttt tgaagccgtt ggcttccagc gcggcgcgcc
     2401 actgctcggg cgtcaggaaa ccgccgttcg gtcgccatgc cggcaccagc accggcgctc
     2461 ggaaggcgcc gagcaaattg aagacgaatt cgacgtagat gggctgatcg gcaaagggcc
     2521 gcacgcattc ggaaatcacc agcgtgccgt tcgccgccaa cgcgttgcgg atttcgctca
     2581 acgtggcggc cagatcgtgc gcgacatgca gcacgttgac gccgtagatc agcgtgcaac
     2641 tcgccggttc gacgtccgcc tccgcgaacg gccggtcgat atccagcttg gcgaaactga
     2701 acggcgcttc cggaaaagcc ggtcgcaagc ttttttgcgc acgccgcaga aacatgcccg
     2761 atatctcggt gaatcgataa gaagcaacgc aattcgtacg cccctcttgt gccaaccgct
     2821 cccagactgc ctgcgcgccg ctgcccagac cgccgcccat ttcgagcaac gcattcccct
     2881 cgccggtcaa ctgttccgcc acgaccaccg cgccgatggc attggaaatg gcatagagcg
     2941 gattgtcgtt ggagaaataa gcgcaccacg catcgaaggt atcggggccg aacagcgccg
     3001 tttcgcccgt cacctcgccg cgcaacaccg ccggataacg ctcggccgca agcgccgcaa
     3061 tcgtataggc ggccaggcat cggttgtcga gtcgctcctg ctcgtgcagc agcggccgcg
     3121 aatccatcgg cgcccaatcg gacgaggcgc ggaattccgg tgtgccgtcc gcgtccacgc
     3181 ggcgttcgac cagaccgttc gccgcaagcg tctccatcaa ccatttgacc ggcacatccg
     3241 cttgcgacat cagattcgcg ctcgacacga gcgatgcgac ggatcggaat tgcccatcgc
     3301 atagccgagc gaggcccaac tcctcgaata cccgtgtcgc gaggcaggtg gtgtattgcg
     3361 caatcagtcc gcaggacgcg atgaacgtct cgccgagcaa cgcccggaca gacgccggca
     3421 ccatcgcttg caaggcctcg atgtggtcgt cgtccgccat ctaagccccc gcaagtcccg
     3481 gcacagggca cagtagagaa cccgctggag aaccatgcat cgcctgctcc tctccgtctc
     3541 tggacaaatc gcctgcctac gcaagaaacg gaccgcgtgc tcacgtggcg aaccgggctg
     3601 cgcgcgccgt cgcctacctt tgctggcgac tctcgccggc agcgctcgtc acggcgtcgt
     3661 cgctacgccg gcgcagactg cgtatcaatc ccggcagata catcccccgc ccgatcagct
     3721 tgacggcaag catgcgggca acgcgggcga tatcgagcac ggggcggaaa taactgccgc
     3781 ggtgatgctt gccgtaactc actgcgatcg gcaccgccac gctgtagatg ccctgccgtc
     3841 cggcctcgat caggatttcg ctttcgaaca cgaaacagga ggcgcgatcg tgacgcaccc
     3901 gtacgcgatc gaacagcgcc agcggataga tgcgaaaacc cgactgactg tcgcgcagcg
     3961 gatagccggc ggcccaggcg atccagaaat tggcgaagcg gttcgcgcga tagcgtgcga
     4021 cggggatctt ctcccgcgca tgcagcctcg agccgatgac gatgtgatcg ggcagcgcct
     4081 cgtgccgctg gatgaggcgc ggaatatcct ccggaaaatg ctgatcgtcg gcatcgagcg
     4141 tcacgacggc gcgcgcacca tccgctttgg cggcctggat gccgcgccag agactggccg
     4201 ccttgcccag gttctcgggg ttgcgcaaca accggatcgg catgtcgcgc agcgcgtccg
     4261 ccgtgccgtc ggtcgagccg tcgtccacta cgatgacccg gatgccttgc gcgagcgtgc
     4321 gttcgaccac attgcgaacg gtacggactt cgttgtacgc gggaatgacg gcgatgtagt
     4381 cggcgaagcg atgcgcatcg caattctgtt caggcaagac caccactcac tgaaataatc
     4441 tgcccggtaa tgtagccggc ttgatccgag gccagaaagc cgatcaaggc cgccacctcc
     4501 ccgggcgtgc cgcggcgctt catcggcacc atgctccgca ccgcggcgtc ggacacggcg
     4561 gcgtcggtgc cgggcgatgc gatcacaccg ggtgcgaccg cattcaccgt cacaccgcgg
     4621 gaagccactt cgagcgcaag cgccttggtc gcgccgtgca gtcccgcttt ggcggccgcg
     4681 taattgacct gcccgcggtt cccgatcagg ccgctgaccg aggacaggtt gacgatcctt
     4741 ccccagcggg tactcagcat gtgcagcagc agcggccgcg tcacgttgaa aaaaccgtcg
     4801 agcgacaccg acacgacgtt gtgccactgt gcctcgctca tccccgccag cgggacgtcg
     4861 tcgtggatgc cggcgttgtt gacgatcacc tggatcggac cgatgcgctc gaccagcgct
     4921 tccagcgcgt cgccgcaaac ctgatgatca cgcacgtcga agcagatcgc ctccgcgctg
     4981 ccgccttcgg cgcgaatggt ctccacgatc cgctcggcac gcgccggatg cgcatgcgca
     5041 tgcaccacca catggagtcc gtcacgcgca agcgtgcgag agatcgcctc gcccagcgcg
     5101 ccgctaccgc cgctcaccaa tgctttcttc atacgtccga gtctaaacgc gcacggtcac
     5161 gtcgagccac cgtccgtcgc cgtaatcgat cgccacgtcg cccgctcgcg gcagcgcgag
     5221 cgattccagc agcggcaggg cctgcgccga cggcacgctg gcgcgcaaga cttcgagttg
     5281 cggatgcgcc atgcgcgtcg cctcgccgcc ctcacgcgcc ccgaagcgca gatcgagcag
     5341 cgcgccgctc gccgccggat ctggcgcgag caccaacgcc acggcgaagc cggattcgat
     5401 cggccgcaag gcatacaacg gcggcggcat gggcagatca tgcgcgacga acatcacggt
     5461 gcgcgattcg gcaaccgcca tgaccgcggc ttccagcaga ccgtgcgcga tcacccagtc
     5521 gtagctgttc accgtgctcg aagcctgcgt gcaggcgccg gcgatggtcc agtagccggc
     5581 gggcgcgttc tgcacggcat tgtgaaagcg cgtcggagaa atctccggct gcggcgcggc
     5641 cagcgcttcg cagaggtgat tgaggttctc cgcatcgcaa tcggacgtgg cgaaaacggt
     5701 tgccacctcg ctcgcctcga cgcccgcatt gcggatcgcc tgcagggcga cgctgagcga
     5761 cgtgcgaatc gcggcgctgg cgcgtcggcg ctctgcaggc gggagaatgg cggcatccgg
     5821 tttgggaacg atcgtcggat cgaaagccgt ctcgccgcgc agtaccgaac gcgccgtcgg
     5881 ccaatccggc atgccgggcg ccagcacgcc cacgccctgg atacggactt gcaccctcat
     5941 gatggcaaac cgatgacgag actgcagtta ttgccgccga aaccgaaggc gttcgtcacc
     6001 acgttgcgca gcctgcgcgg ctcgctgcac aactgaatgt gcgcagcgca ctcgggatcc
     6061 accgcactcg tgttgacggt tcccggcacg aaggcgtgtt tgagcgcgag acaggacacc
     6121 accacgccgg caataccggc cgcgccgagc gtgtggccga aggcgccctt ggtcgaagcc
     6181 accggcgtcg cggcacccaa tacgtcatgg atggcgatgg actccgtccc gtcgttgttg
     6241 cgcgtcgcgg tgccgtgcgc atggacgaag tcgatcgcat cggccgtcag ccccgcacgc
     6301 gacagcgcct gcctcatcgc cgcctgcgca ccgcgccctt ccggatgcgg cgccgacatg
     6361 tgatgcgcat cgctgctttc gccataaccg cgcaccgcaa taccgtccga cgcctcatcc
     6421 gggtcgcgtt ccagcagcgc aaagccggcg gcctcgccga tcgacagacc gtcgcgcgtc
     6481 gcatcgaaag gtttgcaggg ctcccgcgac acgagctgca aggcgttgaa gccgtacagg
     6541 gtggtgcgac acagcgagtc cacaccgccc acgaccgccg catcgcaaat gcccgtgtcg
     6601 atcagtcgct gcgcgcttgc cacgactttc gcgctggacg aacaggccgt ggaaatcgcc
     6661 aggctcggcc cggcgagttg caggtagagc cggacgaact cggcgagcgc gccgaagttg
     6721 tgcgtatggt cgtagcgcaa gtcgttcggc agcgcgccgg tctcggggtc gcggcgctga
     6781 tacgccaact ccgtcgacaa aatgccggac gtgctcgtgc cgagcagaac cgcaacccga
     6841 tcggcgccgt gacgcgcacg tgcgcgcgcc acggcctggg cgaaaccgtc ctgttccagc
     6901 gcgacgagcg ccagacggtg attgcggcaa tcgtagcttt gcagggcggc gggcaaccgg
     6961 acggtttcca gtccgtcgac gcgcccgatg tagcaatcga gcggcgcata ttcgaaatcg
     7021 ttgcgacgca agccggagcg atcttgcgac agcccgtcac gcgtggcagc cagacctgcg
     7081 ccgagcgcgc tgacaaaagt gagggcgttc agatggagcg gtggcacgaa agaaagccaa
     7141 taagatcaag aacaaatagg aagcggcagt atagcagtcg ggttcagccc gaccggccgg
     7201 ccggacgcca taacgcggaa aacaatagtg acagaaccgc ccccgtaccc acggtgatgc
     7261 cgatcgactg cagcaccgga ctcgccgcaa aagccagggc gccaaaggcg ctcgtcgtgg
     7321 tgagcgcaca aaccagcaac gcgaacacat tgcgcgcccg ttcgtggtcg tcgcgcaggt
     7381 tgcgattgaa gaacagcgca tagttcatgc ccacgcccag cacgaggagc agcgacacca
     7441 gatgaaacag attgagccgc gcgccgaagg cggccaacag cgcggcggtg acgaagacgg
     7501 cgcagacgac tggcgacatg acggacagca ccgcgcgccc acggcgcagt ccggcccaga
     7561 gcactgcggc gatggcgagc gcaccgatgc accacagccc cagcgattcg ttgcggtagt
     7621 cgcgcaccat ccggttagac tccgccttca ggtcgagcag gaacaccgat tcgtccgtga
     7681 acgcggcgga cgcatccgcc acgctttgcg catccttgac gccggcaagc ggcagcagcg
     7741 ccagccaccg gtcgccgctt tcgctcagca gcgccttcag cttgagttcc agccccgtac
     7801 ccgcgaggga ctgcggcgtg agcggcggcg cgttgcgcgc ctgttgcacg tccgagatga
     7861 acggttcgaa caggcctgac tgaaagggca agccggtcgc cgcttccgcc agacggctgc
     7921 gcagcgtccg ttcatcgggt agcgccgctt ggcgcgcctg ctgcgccgcc aggctgggca
     7981 gatagaaggc cggagaatcg taggagccga tcaaaccgcg ctgcttcagc tcgtccagcc
     8041 ggctcgctag gcgttcggcg ccgcgcaagg cctcgtcgcg cgtcggcgcc tgcaccgcgt
     8101 agagcagccg cacgtccggc gcgccgagcg cttcgcgcat gcgctgatcc agatcgcgcg
     8161 ccgcctgcgg aatgggattc agattggcga gatcgttctc ccacaattgc gggccacgcg
     8221 tggcaaggaa tgccgcgctg acgatcagcg cgccgatcac cgtccagcgc agccagccgg
     8281 cgagcgcgat ccgcccggca tggagtggcg ggagtgcgcg caccgagcgc agcggcgcac
     8341 tcaccagttg cggcagaacg tagcgcgtca ccaacccggc ggccagcacc ccggacaagg
     8401 aaaacagacc cagttgcgca agcccctgga agctggacag caacatggcc agcgcactga
     8461 agacggtggt caatacggcc aggcgcagcg tcggccagac gcgccgcgcc gcatcgcgca
     8521 ccgtctcgcc cggcgcgatc tgggtgaaga cgtaagtcgg ataatcgacc gcttcgccga
     8581 tcagggtcgc gccgaagccg agcgtgatgc catgcacgtc gccgaaaccc aggctcaccg
     8641 ccgcgatgcc ggtcagcacg ccggtcgcca caggcaacaa acccagcccc agcaacggca
     8701 gcgaacgata ggcaagcagg agaatggtcg cgacgaacag cgtggcaagc atcgacaaac
     8761 gccacgcgtc gctttcgatg gcggcgcgcg actgcactgc gaagacgctc gggccggtat
     8821 agagcaagcg caagtcgtcc tcgccgcgca cgcgctcgaa cgccgcacgg atgtcggcct
     8881 gcacgcgggc ctgcgcatcc atgtcgaaac cggcggcgtg cgtctccgcc accagtaagg
     8941 cgcgacgtcc cgcgtcgtcg aaccacacgc cctcgcgcgt tgccggcccg ctgccgtcca
     9001 tgagcggctg gatgaccgca aagatttcgc ccgtcgggtc gcgggccagg cttgcctgat
     9061 agaacaaccc accggaggac gccaaacgcc ccagggccgc ttccagcgcg gctcgcaagc
     9121 cgtccacgct gaagcgctcg ggaacgacct gatgcgacaa caggtaacgc tgttcgaaca
     9181 ggaattggcg atcgcgttgt cccaactgct gttcgccgtt gttgacgtag ctgaagtgcg
     9241 gggtctgctg cagcgctttg gcaaaggcgc ggctggcggc tgccagccgc ggcgtgtcgc
     9301 cgccctcgat gccgaacagc accaagcgcg aagccacgcc gtcgcgtagc tgatcgacca
     9361 tcaactgctg cttcggggtt gcggagcgcg gtagaaaggc gaccagatcg gcgctgatgg
     9421 tcgtccgcgc gaaaacgatc cacacggcca gcgcgagcag cacgcaccag agcagaacga
     9481 tcgggcgccg gaaaatcacc ggctcgcttc gatgcgggtg atggaacggt tgccgttcgc
     9541 ctcctgcacg tcgatggtat gcaggttgcc gtccgagccg ctgatgcgga tgacgctcac
     9601 gaactgcccc atctcgacct cgcgcggcaa caggctcatg gtccaactgt gctgcgcgcc
     9661 gtcgagctcc acgcgataga acttttgcaa tgtagcgaga tcgccgcgca gcgtggcgcg
     9721 tatgctttcg atgaacgccc agaggaccgg ctggctttgc agcgacaact gatgacgctc
     9781 gccggttacc ttacgctcga aggtcacgcg atcgccgtcg acgatgaaac gctcttcgaa
     9841 cggcgcttcg acaagccgct ccaggtgatc gggcgcttga aagcgcagcg tgccgcgcaa
     9901 ttcgagcggg atcgtcaacg ccgccagatg cttggtttcc gtgaaccgcg cttcagactg
     9961 cgtcgtgcgg ccgagtgcat ccatcagcga ttcgacgttc catgcgcccg acgcgggtgc
    10021 agcccacgac agcgcaagcc acagcaacac gcataatgaa cgctcgatca tccggaccat
    10081 gccatcgact ccagttcgcg cagacgatcc agcggcagtt tgccgctggc ggtgcggggc
    10141 agcgcatcca ctttgatcag cggccgcggc agaaaggccg gatcgataac gggccgcaac
    10201 gccgccatga tgtccgcttc gctcatgccg ggcgccacca cgaaagccat caggcgctgg
    10261 acgcgccggc cgtcgccgcg gggcagatgg aacacgccgt cgcgcacgcc cggtatccga
    10321 ttgaggtggt gattgaggtc gcccagcgaa gcccgcttgc cggcgatatt gaccatatcc
    10381 tgcgcccgac cgaccagact gaagcccgcc tcgccctgca tttcgatcac atcgctgaac
    10441 ggcggacgcc cgttgacgtg gccgcccagt gcgcaccagc cgtcgccgtc gcgctcgagg
    10501 cggatgccgg gcatcaatcg ccaggcgtct tcagtggacg tgcggcgcgt ggcgagcatg
    10561 ccggcttcgg tgaagccgta gatttcacgc agttcgcagc cgtagcggcg ttcaaagtct
    10621 tccgccggtt cgcgcgcgag cggtgcggcc gccgacaccg cgcgtgcgag cggcggcagg
    10681 ctcggccctt cctccagcag tgcgcggata tggatcggcg tggtgatgag cacacgcggc
    10741 gccggcacct gcgcaagaca cgtcgcgaca tcgcgcgcga acagcgggcg gctcgcatgc
    10801 accgcgccgc cggtctgcat cggcagcagc accgtcgatt cgaaaccgta catgtgctgc
    10861 ggcgggacgg tgcccacata ggcataccgt tgcgccgcat cgaggcccag cgcctcgcgc
    10921 tcgctgtgcg caccggccac cagccggccc caggctttgg cattcggccg cggatcgccc
    10981 gtgctgcccg aggtgaagac gagcgccgcc acatgatccg gcgcaatcag cggcactgcg
    11041 tcatcgttcg agcggacgcc cagatcgtcc ggataggcgt gggcatcggc atgacccgct
    11101 tcggcgtgat cgaccacgca gacgtggtcc acgcaggctg cgcgcacctg gccgagcacc
    11161 cgctgtgcat gattgggcgg cagcacgcag gtctggccgt tcaccagcgc cgcgccgaac
    11221 accaccatga agcggtaacg atcgtcgcaa agattgagca cataacgggc tggcggcagc
    11281 cggccggcca gatcgcgcac ctcgcgcaga tagcgcgcgg cgctgatcgg ccccgaggga
    11341 tgccacgcca cgggccggga aagattctcg tgcgcgatga gcggcagtgc attcatggtc
    11401 aacgcagcaa ttcgggcgca ttacgcacca tcagccgtgc gacatgcagc gggctgttgt
    11461 gcgggtaatg acgaaagcga agcaaacgat aggcgtactc acccagaaac agcccgccga
    11521 ttaacacgaa ggtccacacg ttgttgaacc aggaccaggc gtgcggcgtg ccgaacagcg
    11581 ccagcgcgac cgaagtcagg atcaggaacg ccatgagcag cgtccaggcc caggtcacgc
    11641 cccgcgcatg acgcgccaga tcgtcgggca gcacatcgcc gcgatccagc cgggcgatgc
    11701 gtgccaccag tggctcccgt tcgccgacga gcgtacggcc gaacaacatg ccgacacaca
    11761 gatagatgac gaccggcgcc acatacacca tgcccagtgc agcggcgtcg ctcaacgccg
    11821 cggcggcgca gatcagcaag gccaggccga gcaacggcca tcgcagcacg gttgcgaccc
    11881 gcttccacaa taccagcagc ccgacaagca gcgtgcacgc ggcggcgagc gcgggtcggt
    11941 cgctcaacag cgccagatgg agcaacaggg gactgcatac gccaagcgca agggcgacag
    12001 gcctcatgcc gcgctccggc ttacttcgtg cggttcgcct cgatatgctg ggcaaggctg
    12061 gcgaccgaag cgaaaatgcg ttggttgtcg gcatcatcgg cgcggagctg aatgccgtac
    12121 ttcttggaga tggcgagcga aagctcgagc agatcaatag agtccagacc caggccgtcg
    12181 ccgtacaggc cgacttgcgg atcgatctct tcgggcgtca tttccagatt caacgcctcg
    12241 accatcaatg ctgaaagttc cgcctctagc ggcgtcttcg tgctcatacg acctctgtca
    12301 tctgtaatgc ggccggccgc gtgaccggcg aagggcggca agtttaacct ttagagccgc
    12361 ccgcctccat cgacgccgcg tccgtttgca agccgaccgg acgaatgatt tgccgtctgc
    12421 gccgcgccgc ggcccacgcc atccgtggcc gcctcaagct ggtcaggtag taaaccagct
    12481 tgaaaaaacc gatccgccag cgcaccccgt tggcgcgata aatgtctccg gccagcagcg
    12541 ataccatcgc ctcctcgacc cgcagtacat tgcgcgggtt gagcagcagc tcgcgcatgg
    12601 caggtgtggt gatgcgaaaa atgaaccagg agaaggtggc gatgctccgg cgcagtccac
    12661 gctcgtactg cgtcagcgcg gcggcagcct tctccggcct gcgcaggcat tcctccacgg
    12721 tgtccgccgc gcgaaacgcg ctgctcatcg caagatagac gccggtggag aacaccggat
    12781 cgacgaaggc gaacgcatcc cccaccatca ggtggcgggc gccgatggcg cgtctgcatt
    12841 cgtaactgta gttgcccgtc gcggtcaccg gtgcgatgcg ctgcgcgtcg cgcagccgct
    12901 cggcgagcgc cggcaccatc gcaacggtcg catcgagaaa atcctccaga ctgcccttgc
    12961 gctgcttgag gtaatacggc cagcacacgg cgccgacgct ggttatgtcg tgcgtctgcg
    13021 ggatgaacca gaaccagcca tgatcgaacc agtagatggt gatgttgccg gcgtcctcgc
    13081 ccggcaggcg ctgcgcgttc cggtaatgcc cgtagatcgc cgcactcgcg tgcttgcgat
    13141 gcgcctgctt gagcttcagt ttgttgccca ggaaggtatc gcgtcccgaa gcgtcgatga
    13201 gaaagcgcgc gcgcaggtcg cgctcgcccg catcggtgcg aatgccgacc gtcacaccgg
    13261 ccgcgtcgaa ctccgcccgc acgacgcgcg tttcctcgta catgcgcgcc ccccggcggc
    13321 gcgcgttctc gaacagcaac tgatcgagtt cggaacggtg cacctcgaag ccgtagggcc
    13381 aatccttgtc cagcgcgttg gcgaaataga aggtctgttc cttgttgtgg tacatggaac
    13441 tgaactgcgc gccgcgcttg atcatgctga tgcgttcgac ttcctcgagc acccccaggc
    13501 gctcgaacag cgcattgttc tgcggcagca gcgactcgcc gatatggaag cgcgggtgac
    13561 ggtccttctc caacaccgcc acgcaccatc cgcgttccgc aagcagcgca cccgcggtcg
    13621 agccgcccgg cccgccgccg atgacgatga cgtcgtagtc gtaggagtcc tccacctgcc
    13681 tcctttcgga tcagtgatca gcgatcggcc agcaccttcc ggctcgattc ggcaaagatg
    13741 taggcaaaac ccagtccggc gaacagtgaa tttttgcgct tcaccagcgg gctgtcggcc
    13801 atggcactgc catgcagatc gtagtagcgc acgaaagcgc ccatccacca ctttttgtaa
    13861 cggcgcgagc cgcccacgct gacctgcgta ccgctgtaac cgccgtccgc ttcataggcc
    13921 ggccgccccg cacgagcgta ttgcggatcg acgccgtagt agtactcgtg ataggagcgc
    13981 gtcgcgaata ccggaccggt ctggataccc atgttccagg ccgtcccggc aacgttgcgg
    14041 atgtcgaggt tgagcaaggg gttggtcagc cagccgacat gccgcacatg cggccagtcc
    14101 acttcggcca cggtgcgcac cggcaattga aacgtgagtt cgtaatcggc gtgcggcgcg
    14161 cccgccagac gataggtgaa gcgcgggccg acctcgaagg tcgggttgag atcgcgcatc
    14221 ccctcgcgcg cacggttctc gtcgcttttg gccgggttgg cggcactgag gctcaggtcg
    14281 aatttggcgc gcggtcccct gtacagcagc gtttgcgccc cttcgcgatc gacgcgcagg
    14341 aactcgccgc gataaatgaa ataggggagc ggaaagacgt agtgggtgcg ctcgtccgcg
    14401 ccgcgatagt cggggagcga gagtgccccg ataccgacgc ccgcttccca cagggggtat
    14461 tcgtccaggg caagaacggg agcggaaagc agcaggccgg aagcggcgca aaggagtcgg
    14521 aggagtcgca atgtcgtgcc gggcagtgcg ttgcgttgcg ggaacgccgc atgataacgg
    14581 cttgcgccgc cgtgcggcca aagccccctg ctccatcctg cgatttgcga gcgaagggat
    14641 cgcggatcgg tatcagcgca cgaccacgcg cgcgaatttg cgtttcccca cttgcagcac
    14701 cgcattcgtc ccacgcgcaa gcgccagact cctgtcgctc acgcgctcgc cgtcgagctt
    14761 gacgccgccc tggtcgatca tgcgcagcgc ctccgaggtg ctggccgtga gaccggcctg
    14821 cttgagtact tgcgcaatcg gcatcgaatc gtcctgggca tgaagctcga cttccggcat
    14881 gttctccggt gcggcgtgat agcgaaaacg cgcttcgaaa tcccgctcgg ccgcgtccgc
    14941 tgccgcggca tcatgaaagc gcgtgacgat ctccttggcg aacagcacct tgatgtcgcg
    15001 cgggttgcgc ccctgttcga cctccgcttt ccatttgtcc accgtttcca gggacgcaaa
    15061 ggacagcagt tcgatatagc gccacatcag cgtatcggag accgacatga gcttgccgaa
    15121 ctgctccgcc ggcgcctcgg tgatgccgat gtaattgcct agcgacttcg acatcttgtt
    15181 gacgccgtcg agcccctcca acagcggcat ggtgagtacg cactgctgcg gttggccgta
    15241 atccttctgc agttcgcgcc cgaccaacag gttgaacttc tggtccgtgc cacccagttc
    15301 cacgtcggcc ttgagggcga ccgagtcgta gccctggatg agcgggtaga ggaattcgtg
    15361 caccgcgatc ggctgattgg cgcgaaagcg tttggcgaaa tcgtcgcgct cgagcatgcg
    15421 cgcgacggtg taggaggaag cgagcttgat caatccggcg gaaccaagcg cgttgcacca
    15481 ggtcgagttg aaaacgattt cggtgcgggc ggggtcgagg atcttgaata cctggtcgcg
    15541 gtaggtctgc gcattggtct cgacctgctc gcgcgtcagc ggcggccgcg tggcgttgcg
    15601 ccccgacggg tcgccgatca tgccggtgaa atcgccgatc aagaacaaga cggtatggcc
    15661 gaaatcctgg aagtggcgca gcttgttgat caccaccgta tggcccagat gcaggtccgg
    15721 cgcggtgggg tccatgccca atttgacgcg tagcgggcgc ccggttttga gcttgtcgcg
    15781 caattcctgc tcgaccagca actcttcggc gccgcgtttc aagactgtta attgctgctc
    15841 gatatccgac atttatgaca ctttccggtc gagattcctg ttagaatcgc gcgcactttt
    15901 cctttaaaaa catgcggaaa aacaagtttt cgattctatc ccagcaagat gcggcgcgcg
    15961 acagaaagaa atggcgcgtc ggcatcgtgg ggatcgcttt gctgttcggc gggatgaccg
    16021 ccttcggcac ggtgcagcaa tcggacgagc cggagatcaa tccggagacc gtgatcgaag
    16081 aactcgccct gtctttcggc gagtcgctca tcgatgatgg cgatcaagtg tactggcgcg
    16141 aggaacgctt cgctcgcggc gacaccttca tgtccttcct cagccggctc ggggtgaaca
    16201 gcgaggatgc gcgcacgatc gtgcgtcaga acggcggcgc acaattcttc cgcaccatcg
    16261 gcccgggcat gagcgcgcag gccgaaacca atgcacaagg cgatctttac acgttgcgcc
    16321 tgctgagcgg ccgcgattcc gtggtgggcc tgaagcgcga aggcgatcgc ttcacggaat
    16381 tcgacgaggc aattccgctg acgcgcacgg tgcaaatgtc ggccggggcg atctcctcct
    16441 ccctgtttgg ctcaaccgat gcggcggggc tgccggacgc catcgcaacg cagattgcgg
    16501 aaattttcag cggcgacgtc gatttccatc gcgacctgcg ccgcggtgat cgcttctccg
    16561 tcgtgtacga gatgtattac cacggcggcc aggcggtcaa ggcgggccgc gtgctcgccg
    16621 ccgagttcgt caacaacggt cgtaccttcc gcgccgcctg gtacgaggac aaggacggac
    16681 gcggttccta ctacgccccc gacggcaaga gtttgcgcaa agcctttctg cgctcgccgc
    16741 tcgagttctc gcgcgtgacg tccggctttt cgcgacgctt tcacccgatt ctcaaacagt
    16801 ggcgccagca caagggcgtg gactacggcg cgccgaccgg tacccgtatc cgcgccactg
    16861 ccgatggggt cgtcgatttc gtcggccgca agggcggata cggcaatacc gtagtcctgc
    16921 gccatcacgc cggcatcacc acgctgtacg cgcacatgaa cggctttgcg aaaggcattc
    16981 gccgcggcgc gcgggtcgag cagggacagg tcatcggtta tgtcggcgcc accggctggg
    17041 ccaccggccc gcatctgcat tacgaattcc acgtccacgg cgtgcaccgc aatccgctga
    17101 cgatcgccat gccttcggcc gacccgctgc cggcacgcaa gattcgcgag ttcaaggaac
    17161 aggtccagcc ggttcttgcg caactcgatt tcctgagcga cgtgcgcgtc gcgtccatcg
    17221 actgacccac cgcagtgagc gcgctgtaca tcgggctgat gtcaggaacc agcctcgatg
    17281 gggtcgatgc cgcgctggtc gatttcgccg gcgaccgtcc gtcagtccgc ggcacccgtt
    17341 tccttcccta tccggattcg atccgtgccg aagcgctggc gctcaatagc cccggcgacg
    17401 atgaaatcca ccgcagcgcc ctgctctccc gcgaactgag ccggctgtat gccgaagcgg
    17461 tcgaactgct gctgggcgaa tgcggtgtga tgcctcgcgc cgtcgcggca atcggctgcc
    17521 acggccagac ggtgcgccac cgcgccgatc tgggctatac ggtgcaactg gtcgaccccg
    17581 ccctgctggt cgaacgcacc ggcattcccg tcgtcgccaa cttccgcgcc cgcgatctgg
    17641 ctgcggaagg ccagggcgcg ccgctggtgc cggcgtttca tgcgggcgca ttccgccatg
    17701 ccgatcgcga tcgcgtgatt ctcaatgtgg ggggcatcgc caacatcacg gcattgcccg
    17761 catccggcac gatcatcggc ttcgacaccg ggccggggaa tgtgctgctc gacatgtggt
    17821 gcgcacgcca gcgcggctgc gcctttgatg ccgacggcga gtgggccgca agcggccgcg
    17881 tcgttgaagc tctgctcgac gatatgctcg ccgaccctta tttcgcgctg ccgccgccga
    17941 agagcaccgg ccgcgatctg tacaacgagg cgtggctgat gcgtagggat ttggcccgat
    18001 acgcggccga agatgtgcag gcaacgctgc tggaactgac ggcgcgctcc gtcgcggacg
    18061 cgatcgaacg atattgtccg ggcgcggacg agctttatgt ttgcggcggc ggtgcttcga
    18121 accgccagct tctgcgacgt ctgcaggcgc tgttgggcgg cgtgtcggtc tccaccaccg
    18181 ccgacctggg cctgcacccg gactgggtgg aggccgtcgc ctttgcctgg ctcgcccgcc
    18241 gcacattgct gcaacagccc ggcaatcttc ccgaagtcac cggtgcgcaa ggcccccggg
    18301 tgttgggagc ggtctatccg acctgagccg gcgcaacgac gacaggcatg aaaaaagcgg
    18361 cccgcaagcc gcttttttgt tgggcgagaa actcttacgc cgagaaggag gaaccgcaac
    18421 cgcaggtcgt ctgcgcattg gggttcttga tgacgaactg cgccccttcc aggccttcgg
    18481 tgtagtcgat ttccgcgccc accaggtact ggtagctcat cggatcgatc aacagcgaaa
    18541 cgccgttctt ctccatgacc gtgtcgtctt cgttcgcgac ttcatcgaag gtaaatccgt
    18601 actggaagcc cgaacagccg ccgccgctga caaatacgcg cagcttgagt tcgctgttgc
    18661 cttcctcgtc gatcagctgc ttcaccttcg aagccgcgct gtcggtaaag atcaagggtg
    18721 ccggcatatc ggtggccgtg ttcatttact gctctccttg ttgcgtaggc aacgattatg
    18781 tgcgacgcgg cgcgccgggg tcaacgcggt gcgccgcagg atcgcgactt ttaagctccc
    18841 tcgtctgagc gcccgggtgc ccgaaggttc cgcggaccct agggcagcac cgggagttgg
    18901 tccagccccg tccgttcctc gaagccgcac atgatgttca gattctgcac cgcctggccc
    18961 gcagcgccct tgaccaggtt gtcgatcacg gacaggacga cgagggtgtc gccgccctgc
    19021 ggccggtgaa cggccatgcg gcacatattg gcggcacgga ccgagcgcgt gtccggatgc
    19081 cgtccgggcg gcagcacgtc gacgaagggc tcgtcgcgat agcgatcctc gtacaacttc
    19141 tggaaatccg cctctttggt gatgcgggcg tagagcgtcg catgaatgcc gcgaatcatc
    19201 ggcgtcaggt gcggcacgaa ggtcaacccg acgggtttgc cggcagcgcg cgccagccct
    19261 tgccgcactt cgggcagatg gcgatggccc gctacgccgt aagcggaaaa attgtcgccg
    19321 gcctcggcga aaagaagatg ggtttccgcc ttgcgccccg cgccggatac gccggatttg
    19381 acgtcggcga tgagatggtc gaggtcgatc acgcccgctt ccacaagcgg caggaagccc
    19441 agctgcgtgg cagtgggata gcagcccggg ttggcgatca gccgcgcatt ccggatctgc
    19501 tcgcgattga cttcgggtag gccataaacc gcctcggcga gcagttcggg cgccgcatgc
    19561 ggaagcttgt accacttctc ccactcggcg atgtccttca gccggaaatc ggcagcaagg
    19621 tcgatcacgc gcacaccggc gtcgagcagc gtacgcacct cctgcatggc cacaccgttg
    19681 ggtgtggcgt agaacacgac gtcgcacttt tccaggccgg cattcgcagg ttcgctgaag
    19741 gccaggctca cgcggccgcg cagcgagggg aacatgtcgg tcaccggcag ccccgcgtcc
    19801 ttgcgggagg tgatggcttt cagttcgact tccggatgct gggcgagcag gcgcagcaat
    19861 tccacacccg tgtaccctgt tccaccgacg ataccgacct tgatcatgtc cgcctttttc
    19921 ctcttgaccg tataacccgt taccgcagac aaaaaagccg cctcgagggc ggcttttttg
    19981 ggatgcgcaa aagcgcgctt aacgcttgga gaactgcttg cgacgtcgcg ccttgtggaa
    20041 gccgaccttc ttgcgttcga cttcacgcgc gtcgcgcgtc accagaccgg cgttggacag
    20101 cgtcggcttg agttctgcat cgtattcgat caaggcacgc gtaatgccgt gacgcaccgc
    20161 accggcctga ccgaattcgc cgccgccatg gacgttgacc ttgatgtcga agccgttgag
    20221 tgcatcgatc agttcgagcg gctggcgcac gaccatgcgg ccggtttcgc ggccgaaata
    20281 atcgtccacc ggcttgccgt tgacgatgaa ctggcccttg ccgggcttga tgaacacacg
    20341 cgccaccgcg ctcttgcggc ggccggtgcc gtagtagtac tcgccgacca ttagttcagc
    20401 tccagatttt gaggctgctg cgcggaatgg ggatggacgt catccgcgta gaccttgagt
    20461 ttcttcgcca tcgcataacc gagcggcccc ttgggcagca tgcccttgac ggccttctcc
    20521 aacacgcgcg cgggataacg accatgcagc ttttcgaagg tcgtctcata gataccgccc
    20581 gggtagccgg tgtggcgata gtactttttc tgcagggcct tgttgccggt cacgcgcagt
    20641 ttggccgcgt tgacgacaac gatgtaatcg ccagtgtcga catggggcgt gtattcgggc
    20701 ttgtgcttgc cgcgcagacg cagggcgatt tgggcagcaa gccgaccgag caccttgtcc
    20761 gaggcatcca cgacaaacca atcgcggcgc acttcatgcg gcttggcgga aaacgtcttc
    20821 atgattcaga ttcttaagtt tgtactgcgc agaggaagcg cgggatttta agtggactgt
    20881 agaagtctgt caaaccaact gcacgtccct aaaaaaaacg cagtcccgag ggactgcgtt
    20941 taaaaatcca ccaaggagga ggaggtggag gagacgcccg acgattgcca gaggaggagg
    21001 gaaagcaacc gccgggactg agcgtattgt ggagcagcat tttgtgcagt gcaaagaaaa
    21061 tttgtgcatg gcagcataag taaacccatt ggaacctatt aatggggcgg gttacggccc
    21121 gtgcgactac aatcgcggct ttcaaagatt gggtttgcgc catggaatgc acggtcaagt
    21181 gggtggatgg aatgagtttt gtggccgaga cgggaagtgg tcacatactt aacatggacg
    21241 gtgcacccga aggcggcggc cgtaatcttg cgccacgtcc catggaaacc gtcctggccg
    21301 gtgccggcgg ttgcacaacc tacgacgtcg tgctgatcct caaacgcagt aatcaggatg
    21361 tgcgcggctg cgaagtccgg gtcgacgccg agcgcgccga aaccgatccc aaagtgttca
    21421 ccaaaattca catgcacttc atcatccgcg gccgcaacct ccgcccagcc ctggtggaga
    21481 gcgccatcaa gctttcgcat accaagtatt gctccgccac gctgatgctg ggcaaaaccg
    21541 ccgaaatcac tcacgactac gaaatcatcg acgacgcgtt gtag
//
LOCUS       DATGOU010000005        31133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_906859, whole genome shotgun sequence.
ACCESSION   DATGOU010000005 DATGOU010000000
VERSION     DATGOU010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 31133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31133
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906859"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VKZ48_00235"
     CDS             <1..599
                     /locus_tag="VKZ48_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HLU76309.1"
                     /translation="RQFLHRCNIGSESMSPEEQARVADIGKRYRWRDVTGHTVHFLTD
                     DEIKNLSIPHGTLTDDEREIINYHIVSTINMLESLPWPKHLRNVPEFAGGHHERMDGR
                     GYPRGLKGDQMSVQARVMAIADIFEALTAKDRPYKRGKTLSESLQILGKFKLNGHIDP
                     DLFDIFIREKVYLRYAEQFIDREQIDEVDESRIPGYDG"
     gene            885..1352
                     /locus_tag="VKZ48_00240"
     CDS             885..1352
                     /locus_tag="VKZ48_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HLU76310.1"
                     /translation="MKAQKGFTLIELMIVVAIIGILAAVALPAYQDYTKRAKMSEVIL
                     AASACRTTITEVYQSGDTGPGANNWGCEPSSPPSKYVQSIATDENGVVTVTATGFNDN
                     QIDNRVVTLVPYVNNNAPMTAANDMGKGVFKWVCGDTGTTIAAKYLPGSCKGV"
     gene            1483..3033
                     /locus_tag="VKZ48_00245"
     CDS             1483..3033
                     /locus_tag="VKZ48_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HLU76311.1"
                     /translation="MLTIPILCALSAVVYAAWMRAQKPVYLFALVVFLLSTILSLVYF
                     VSDDITGEGITEATLFHLMYGFDGIDLAGFSVYVALSIICLVAIPGIGYFGWKSCTRQ
                     TRRGPSYRPSVIVHLTVIGALSVFAVGVHPATLDTMQVVEKFNDARYNGLLSEELREL
                     DGIAPPKRGKSLVYIYAESMERTFFDKRIFPGLIQELAKLEESSLSIRGIGQAPLTDW
                     TIAGMVASQCGIPLATFRSNRNDLGDLKSFMPGATCLGDILNQAGYRLAYMGGADLSF
                     AGKGQFYRDHSFSDVTGLKELQNRLGEDIPLSKWGVYDDRLFEQAVEKFRELSKTGEP
                     FALIMLTLDTHAPTGHETPTCARDPNLGYRDGSSKMLNAVKCSDRLIARFVEQIEAEA
                     GNDVVIVVASDHWQMRSDAAEPLSRSPVRENLFLVRGAGIEPGVISKPSTTMDIAPTV
                     LSLLGWDISTFALGRNLLRDEPTLVEKFGRAQYYDMVKSWRMNLWRVWEGQSQSDSAA
                     PALEDGAA"
     gene            complement(3106..4113)
                     /locus_tag="VKZ48_00250"
     CDS             complement(3106..4113)
                     /locus_tag="VKZ48_00250"
                     /inference="COORDINATES: protein motif:HMM:NF024498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HLU76312.1"
                     /translation="MLAALAILAVVGSGAVRAQNASPHAIQIPGWFAPSFLDFREDVD
                     DARRQGKRVMLYIGQDGCPYCARLMQVNFKQPDIVAYTREHFIAIELNMWGDRETVWL
                     DGVARAEKDLAKALDVQFTPTLLFLNEEGDIIARINGYYEPARFMAALRYAGEHLESK
                     IGFAEHLQQATAKPKAKMKSGFVDQPFFIKPPYDLRRKPEGKPLAVLVEAPSCETCAE
                     LHEKGFPNPQVREQIDKLDIARFALDERSPITTPDGQSTTAADWLATQRITYTPSVLF
                     FDQYGKAVFRLEAYVGPFHLASAFDYISSGAYLKEPSFQRYLQRRADELRAQGRAVIM
                     D"
     gene            complement(4205..4831)
                     /gene="soxX"
                     /locus_tag="VKZ48_00255"
     CDS             complement(4205..4831)
                     /gene="soxX"
                     /locus_tag="VKZ48_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016722444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HLU76313.1"
                     /translation="MKKTMMLATLALSLGASAVHAGDKSSDPVALKKATALMKQDFKS
                     TGIATVDRLDQDQAMAVCTKYRGDPPADIARELQAAQMALIKYPADGKYMGDWKNGDK
                     IAKSGRGATWRDKPDTVNGGGCYNCHRVSGTELSYGTIGPSLYQFGKLRGGPTEANMK
                     YVYGKLYDSQAYVPCSNMPRFGYHGVLTEQQMKDLTALLLDPESVVNQ"
     gene            complement(4843..5667)
                     /gene="soxA"
                     /locus_tag="VKZ48_00260"
     CDS             complement(4843..5667)
                     /gene="soxA"
                     /locus_tag="VKZ48_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HLU76314.1"
                     /translation="MATLRRSLAQGICAFALLVPSALLAQEDKDSTAAAIEMYREMLA
                     DGNPAELHELRGEELWTTPRGPKNASLEKCDLGLGPGKVQGAYAQLPRYFPDTDKVED
                     LESRLVTCMTTLQGFDREEVTKKPYGSISYQADMEALAAYVVGQSRGLPMAVPQKHPK
                     EKEAYAIGKEIFYYRAGPYDFACATCHGEDGKRIRLQDLPNLTEKKGAQFAYTTWPAY
                     RVSQGELRTMQWRLWDCFRQQRFPEPKYISDATIALTTFLAVNANGAKYDGPAIKR"
     gene            complement(5732..6049)
                     /gene="soxZ"
                     /locus_tag="VKZ48_00265"
     CDS             complement(5732..6049)
                     /gene="soxZ"
                     /locus_tag="VKZ48_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HLU76315.1"
                     /translation="MANPMKIRAKLEGDVANVRVLMSHPMETGQRKNAAGELVPMHFI
                     KDIRAELNGKPVFEAAISQAVSRNPVFAFKVKGAKAGDKLSITWEDNRGDKRTDTVEL
                     GGA"
     gene            complement(6078..6548)
                     /gene="soxY"
                     /locus_tag="VKZ48_00270"
     CDS             complement(6078..6548)
                     /gene="soxY"
                     /locus_tag="VKZ48_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier protein SoxY"
                     /protein_id="HLU76316.1"
                     /translation="MNRNRRVALKSAGLSSMLVAGFATGLLRPADALAIEWNQDAFHA
                     KNVADALQKLGAQNPVDSGDITIKAPDIAENGAVVPIDITSDIPGTERITVLIDKNPF
                     PYVGSFDFTKGALPYVHVRVKMGESSNVRVIATANGKHYQAVKEVKVTIGGCGG"
     gene            complement(6719..7198)
                     /gene="moaC"
                     /locus_tag="VKZ48_00275"
     CDS             complement(6719..7198)
                     /gene="moaC"
                     /locus_tag="VKZ48_00275"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HLU76317.1"
                     /translation="MSLTHFDADGQAHMVDVGDKAETHRIARASGTLHMRPETLELIR
                     SGSAKKGDVLGVARIAAIQGAKRTADLIPLCHPLALTKIGTEFTVDAEASTVTLQVTV
                     ETRGRTGVEMEALTAVSVGLLTVYDMCKAVDRGMRIVGVKLLEKQGGKSGHFRHPDA"
     gene            7315..8760
                     /locus_tag="VKZ48_00280"
     CDS             7315..8760
                     /locus_tag="VKZ48_00280"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HLU76318.1"
                     /translation="MRFLLSLLLSTALALPPFVHAQNLPDLGESSQAEFSPHAERELG
                     QSIMSRIRRDPAYIDDPEVAAYISGIGQRLAAATPETAGIDFHFFAVRDPNINAFALP
                     GGYIGVHSGLVTAAMTESEFASVLAHEIAHVSQRHIARQLQAQSQIGALSMIGMVIAM
                     LAASSNPQVAEAAAVAGSAAPVAAFLNYSREFEREADRLGYQILRAAEFDVHAMPSFF
                     ERLQRSTRLYENNAPGYLRTHPLTTERIADMQHRTREERPARMQPDSRTFQIVRAKLR
                     AEQGDASDAAAFFREALADRRYADRGAARYGYVVALLRMGDVATARAQWLELRKAADS
                     HPMYETVGARVYLAAGEPEEAVRVLQRAVKKHPHNDLVRVAYADVLQQAHRHQEAVSL
                     LSTLRSERPGEPRFYQMLAKSYAGLGQRVEQHRALAEYYYLTGSLPGAIQQLELAQSA
                     KDADFYTLSAVDARLRQLRAQQLQEMKERQR"
     gene            complement(8783..9748)
                     /locus_tag="VKZ48_00285"
     CDS             complement(8783..9748)
                     /locus_tag="VKZ48_00285"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HLU76319.1"
                     /translation="MPTPLQPSSSRWLALIVHLPQDATGTRMAMLRTLETLGAAALRK
                     GVYLLPDTPDHARSLQRLANYLIGAEASCEVLAVESLDPVQTKRIRALFDRSAQYGQL
                     LQTLGSLESSIGMADPAALGRVLAKQRRELTRIRSLDFYGSPQAVQVEERLGALEERV
                     HRLIFPDVTQPSTARPKRYLQRVWATRRPLYADRLASAWLIRRFIDPEATMLWLDHDD
                     ESPLDAVTYGYEGASFQNSRARVTYEELLAYCGRQNEPALARIGILVRALDVGDRQLA
                     EAVGVETLLDGARRRAANDHDLLQECEMTFDLLYEAYLDAPSRKS"
     gene            9880..10107
                     /locus_tag="VKZ48_00290"
     CDS             9880..10107
                     /locus_tag="VKZ48_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003263554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HLU76320.1"
                     /translation="MDFDKDLDARGLNCPLPILRTKKALAEMSAGQVLRILATDPGSV
                     KDFEAFAKQTGNTLLEHGEANNEFTFYMRKK"
     gene            complement(10123..11793)
                     /locus_tag="VKZ48_00295"
     CDS             complement(10123..11793)
                     /locus_tag="VKZ48_00295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLU76321.1"
                     /translation="MRWPRLTLSIRSKLLLVSLVLLAIPLVGYSFVREMENVLRTGQE
                     QVLLATARAVATTLHDRPELLERRAVAAGDDASLAAAPSTGEIEQIVKSLRRSSSRIW
                     VIDRQHRLLALEGGLRRDSLEATEREPGLWERIAEPVLRPLYKLLLERPREDFDDALP
                     EQVLSGGREVYSALSGVPASRWRQTTDQRAVILSAAHPIWVGDEVVGAVVVEETTNAV
                     LSLRNRAIEQLIAVTLAVFLLGAGTLLFFASSLSRRLQRLRDSAEHAIDAQGRVMHGL
                     PAASDGDEIGDLSRSFSAMLERLADYTTYLERMADRLSHELRTPVAVVSSSLENLKSE
                     TVPDSARVYITRAEEGVKRLNLILTRIAEATRLEQMLRHADRERFDLGRVVSGCVAGY
                     AGAFPAFRFDLHLPERRIEIDGSPDLIAQALDKLVENAMDFSEAGRPIEVSVALTDGE
                     ARLAVANEGPELPAAMQGRLFESMISMRRDAATERRNTPHLGLGLFIVRLIAEFHHGR
                     AMARNREDRRGVVVQMVLPLPNPEKSGATDTPNRAGAHARPDLNKADN"
     gene            complement(11797..12489)
                     /gene="pdsR"
                     /locus_tag="VKZ48_00300"
     CDS             complement(11797..12489)
                     /gene="pdsR"
                     /locus_tag="VKZ48_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR03787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteobacterial dedicated sortase system
                     response regulator"
                     /protein_id="HLU76322.1"
                     /translation="MSRRIAIVEDDPAIRENVADALRRQGYELSTYPDRQQALAAFKT
                     RLPDLAIVDIGLGAEVDGGFTLVRELRALSETLPIIFLTARDSDFDLVAGLRLGADDY
                     LTKDVSMPHLLARIAALFRRVDVLSTPDHAEELVERGHLRLDLKRMTAQWRGRPVDLT
                     LTEFWMVNALVKYPGHVKSREQLMSEANLVVDDTTVTSHIKRMRKKFLAVDPDFDAID
                     TVYGMGYRWTAQ"
     gene            12663..14801
                     /locus_tag="VKZ48_00305"
     CDS             12663..14801
                     /locus_tag="VKZ48_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="marine proteobacterial sortase target protein"
                     /protein_id="HLU76323.1"
                     /translation="MTARSDRYPRHAGRLADFLETLSLVLVTGALTGVGFIVAAVLLT
                     WATAAFAGESAEAAPAGVLTLRTADGKSVASAPRLATEVRMRITGMVARVRVVQRFEN
                     PYDDWYEGVYVFPLPDNAAVDTLQMRVGDRLVQGVIKERQAAKRSYEQAKARGSRAAL
                     LEQERPNIFTTSVANIGPRETVSVEIEYQQAVRYADGRFSLRFPLVVAPRYIPGLPLD
                     ASPGGSGWARDTTQVPDAARITPPVLHPRKLPDDGVLNPVDIAIELDAGIPLADVASS
                     SHDLRVERRSERAFRITPARAHIAADRDFTLDWIPKQGAAPYAAFFRERHGDADYGLL
                     MIMPPGGDATRTRLPREVVFVIDTSGSMAGASIEQAREALMLALRRLTPTDRFNVIEF
                     NSVTRAFAAEALPATPARIRRALAWVAALQAQGGTEMAEALRTALTASRDPHLLRQVI
                     FLTDAGVGNETELYALIRERLGDSRLFTVGIGSAPNGYFMRKAAEVGRGTYTYIGRTD
                     EVARKMGELYAKLESPAMKNLRVQWPSGVAVETYPTRLPDLYHGEPLLLTVRAPELSG
                     PLRVEGEQGALRWSQTLPLRHGVPNAGIARLWARAKIDALMDGVHEGRSLEAVKRDVI
                     DVALDHDLVSRYTSLVAVEQMPVRPADAALKPGVVPNQLPEGWVHEAVFGPLPQTATP
                     AAVHRLAGAIALLLAALLYFLSWRRAHAYP"
     gene            14788..15375
                     /locus_tag="VKZ48_00310"
     CDS             14788..15375
                     /locus_tag="VKZ48_00310"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class GN sortase"
                     /protein_id="HLU76324.1"
                     /translation="MRIPKFHALQWAAAVLLAVALWQFGHAGYIHAKAVLAQSLIHNA
                     WIRTQATGEPVKPWPWADTWPVARLQVPRLGVELYVLAGASGRTLAFGPGHLYGTAAP
                     GETGNSVIAAHRDTHFAFLRDLRAGDEVIVETLTGRVTRYAVSGARIVDEYDRDVLEQ
                     DAVAARLTLVTCWPFDAVVPNGPLRYVVSAQVRAG"
     gene            complement(15357..16634)
                     /locus_tag="VKZ48_00315"
     CDS             complement(15357..16634)
                     /locus_tag="VKZ48_00315"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLU76325.1"
                     /translation="MHRQAAMNHALRSLRHPNFRLFFFGQFISLLGTWLQIVATVWLV
                     YRLTGSALLLGVTTGLQQLPMLLLSPLAGVWSDRVDRRRMLMITQSLAAFQALTLAML
                     TFSGTVRVEHIMLLAFLLGLINAFDTPTRQAFVLEMIGDKSDLPNAIALSSLLFNGAR
                     FVGPAIAGVALAVLGEAWCFLLNGLSFVATLYAYTHMRLLPRAWTARNAHWLHDLQEG
                     VRYAFGLLGIRRLLLLLAVLSIGSSPWQPLMPIFATEIYGGDSRTLGWLIGAVGVGAL
                     LGAAHLMRRPGLRGLGSVIAVTCALSGVGMAGFAWMNSLWLGLPLLALFGFGLIATVA
                     SCNTVVQTVAEEDKRGRVIGLYVLAFFGMSPIGHFLSGALAEQIGVQWTFFLCGSGIA
                     IAAACFAYSLPTWRESIRRARAAQDASGQPART"
     gene            complement(16804..19584)
                     /locus_tag="VKZ48_00320"
     CDS             complement(16804..19584)
                     /locus_tag="VKZ48_00320"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HLU76326.1"
                     /translation="MELAKSFDPHAIEQHWYPHWESQGYFKAGFEPGRPAYAILLPPP
                     NVTGTLHMGHAFQHTLMDALTRFHRMMGWNTLWQPGTDHAGIATQIVVERQLEAQKLS
                     RHDLGREKFIERVWEWKEQSGSTITRQMRRMGASCDWSRERFTMDEGLSQTVTEVFVR
                     MYREGLIYRGKRLVNWDPKLGTAVSDLEVVSEEESGSLWHIRYPRVDGKGHLVVATTR
                     PETLLGDVAVAVHPEDERYKDLVGKAVQLPLTGRTIPVIADEYVDREFGTGCVKITPA
                     HDFNDWQVGQRHGLEAINVMTLDARINDNAPEAYRGLDRFEARKRIVADLERDGLLES
                     IQPHKLMVPRGDRTGAVIEPMLTDQWFMKMDMLAKLGLECTHAEEGDPEVRFVPENWL
                     TTYTQWLTNIQDWCISRQLWWGHRIPAWYDDEGRVYVAHDEAEAREQAGGKALRRDED
                     VLDTWFSSALWPFSTLDWTPDYPEKSNPALDTYLPTSVLVTGFDIIFFWVARMVMMTK
                     HITGKVPFREVYVTGLVRDAEGQKMSKSKGNILDPLDIIDGIELNALIEKRTFGLMNP
                     KQAEQIEKATRRQFPNGIPSYGTDALRFTFASLATHGRDIKFDLARCEGYRNFCNKLW
                     NATRFVLMNTEGKDCGQDESLSLELSFVDRWIISRLQRAESQVHDAFRDYRFDQVSRA
                     IYEFVWDEYCDWYVELAKVQLQSGNDAQQRATRRTLVRVLEAVLRLAHPVIPFITEEL
                     WQKVAPLTTADGKLPADSIMLAAYPRPRSERLDEEAEQQVAELKALTDACRNLRGEMN
                     ISPSQRVPLLAEGDATTLTRYFPYMQLLARLSEASVVNPLPEADAPVSVVGTTRLMLE
                     VEVDADAERERLSKEVARLEAEIAKAQAKLSNASFVERAPANVVAQEKDRLANFSATL
                     EKLRPQLDKLKA"
     gene            complement(19911..20309)
                     /locus_tag="VKZ48_00325"
     CDS             complement(19911..20309)
                     /locus_tag="VKZ48_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76327.1"
                     /translation="MSDSPEDVLRKAEALLARHRMPRTERQPAPPVDFPTLTEVVEEA
                     PPRPGEGGVPMSESELANFERELRTEILQLVRAELERLVEARLHPRIESTVAEVMTRA
                     RVDLEVEVRRAVREAVTQVIDDEIKRLNAE"
     gene            complement(20329..20763)
                     /locus_tag="VKZ48_00330"
     CDS             complement(20329..20763)
                     /locus_tag="VKZ48_00330"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HLU76328.1"
                     /translation="MTRIDFYHDAEDRLAIACRLVAKAVQQKLRVVVYSPDAAVADTV
                     DRMLWTTPPIAFVPHCRAGHRLAAETPVLISADDDHASTHEDVLVNLHDEWPPSFARF
                     HRLIEIVGREDADRQHARARFRFYKDRGYPIHAHNLGERATA"
     gene            complement(20760..22250)
                     /locus_tag="VKZ48_00335"
     CDS             complement(20760..22250)
                     /locus_tag="VKZ48_00335"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HLU76329.1"
                     /translation="MEFSTKVAEPNKLATGALVVGMYESGKLSDSAAAVDKAAKGLIT
                     ELVKRKDVTGKAGSTRVLLKPANLPCDRLILVGLGKEAEFGAKAYRDAVRAAANAIKD
                     TGAADAHLYLVELPVRGCDLRWTARQAVMVTAEVFYRFEEFKSKKSPAATLKKFVVAL
                     SEKSVLNAINEGVAEGRAIAAGMELAKNLGNRPSNLATPSHLAAQAQQLAKTYKLGVE
                     VLERKDMEKLGMGALLAVAKGSHEPPKFLVLQYNGGAKKDKPVVLVGKGVTFDTGGIS
                     IKPSAEMDEMKYDMCGAASVLGTFRAIAELKPKLNLVGLIPATENMPGGGATKPGDIV
                     KSLSGQTIEILNTDAEGRLILCDALTYAERFNPAAVVDIATLTGACVIALGHVATGVF
                     ANDEGLAESLLEAGRESWDRGWRMPVWEDYQEQLKSPFADMANIGGRPGGSITAACFL
                     ARYAEKYPWAHLDIAGTAWTSGKNKGATGRPVPLLTTYLLHRAGQS"
     gene            22406..23488
                     /gene="lptF"
                     /locus_tag="VKZ48_00340"
     CDS             22406..23488
                     /gene="lptF"
                     /locus_tag="VKZ48_00340"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HLU76330.1"
                     /translation="MIFQRSLLREFGNYAAAVFAALFAIIVTSQLVRMLGRAAGGSIP
                     ADAVFALIGFTAINYLPVLLALTLFVAVLMTVSRAYRDSEMVIWSASGVSLLAWIRPV
                     LMFAAPLVAVIALMALLLSPWANNRADEYRRQIESRDEVSRIAPGVFRESANAERVFF
                     VESADVDDGRVKNVFIASKQHGKTGVVVSKEGHIEVHDNGDRFAVLLNGRRYEGLPGT
                     PEYRITEFERYAARIQSGEQQAGRGSPKTLSTAELIRDPTPRGQAELVWRIGLPFVAV
                     NLALLAIPLGFVNPRASRSVNLLFAVFAYMLYNNLISVVQSWVSQEKLSFGIGWWVLH
                     AVALGVVALLFAWRMSLGFAFLRRPR"
     gene            23485..24558
                     /gene="lptG"
                     /locus_tag="VKZ48_00345"
     CDS             23485..24558
                     /gene="lptG"
                     /locus_tag="VKZ48_00345"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HLU76331.1"
                     /translation="MTIITRYLSREIYKATAFVLFAFLALFAFFDLINELDHLGKGTY
                     GLPQAFLFVMLSLPGHIYELFPIVVLIGTLVALSNLAHHSEYTVLRVSGFSPWRAGAT
                     LTRIGMAFVIAAILVGELAAPAAEQTAQRLRLDSLGRTVAQELRTGLWVRADSKFVNI
                     QEVSPDSRLKGVRIYEFDEQMHLRSISEAAAGEYLNGSTWRLSDVQQTQFTADGAHVE
                     KLGTIEWKTVITPDMLAVLLVRPEKMSAWALYQYTRHLAKNNQRTERYEIALWKKVVY
                     PFAALVMMGLALPFAYVLVRAGGVGAKLFAGIMLGVLFHFLNTLFSHLGVLQGWPPFL
                     SATLPSAIFLLIAVGMMRWVERR"
     gene            complement(24718..25260)
                     /locus_tag="VKZ48_00350"
     CDS             complement(24718..25260)
                     /locus_tag="VKZ48_00350"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HLU76332.1"
                     /translation="MKQDFSSPSPSDQRLAGIYPSIARRLAAMVYEALIVTAILLGAA
                     FLFVGAATGELHGGVRLTFQLYLLLILGAYFVWCWHKGRTLAMRAWKLRVVRPDGGPL
                     SMWQALLRFVLAALTLGGGVVGAIALWKQHDLIGAWLAVGVGLFSLAWALLDRDDQFL
                     HDRLAGTRLVLVKEPRKSKP"
     gene            25555..27288
                     /locus_tag="VKZ48_00355"
     CDS             25555..27288
                     /locus_tag="VKZ48_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 catalytic subunit"
                     /protein_id="HLU76333.1"
                     /translation="MPTAKEPRAPALTGADIVIKCLEAEGVEYVFGYPGGAVLYIYDA
                     LFKQDKVKHVLVRHEQAATHAADGYSRASNKVGVALVTSGPGVTNAVTGIATAYLDSI
                     PMVVISGQVPTHAIGLDAFQEVDTVGITRPCVKHNFLVKRVEDIAPTLKKAFHVATTG
                     RPGPVLVDIPKDVTTMVHPFNYPEKVEMRSYNPVLKGHPGQIKKAAQLILEAKRPYVY
                     AGGGVILAHAADELTKLVKKLGYPCTNTLMGLGAFPATDRQFVGMLGMHGTYEANMAM
                     LHCDVLIAIGARFDDRVIGNTAHFGQVPRKIIHIDIDPSSISKHVKVDVPIVGDVKET
                     LEELLSHIDGKTSKIDQKALQAWWAQIELWRGKDCLRYDRTSDIIKPQFVIEKFYELT
                     GGDAFVCSDVGQHQMWAAQFYKFDKPRRWINSGGLGTMGFGLPAAMGAFLANPDATVA
                     CVTGESSIQMCIQELSTCRQYHLPIKILNLNNRYMGMVRQWQQFFHGNRYAESYMDAL
                     PDFVKLTESYGHVGMRIEKPGDVEGALREALKLKDRLVFMDFMTDQTENVYPMVAAGK
                     GISEMILAEEL"
     gene            27341..27832
                     /gene="ilvN"
                     /locus_tag="VKZ48_00360"
     CDS             27341..27832
                     /gene="ilvN"
                     /locus_tag="VKZ48_00360"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HLU76334.1"
                     /translation="MRHIISVLLENESGALSRVAGLFSARGYNIESLTVAPTEDASLS
                     RITLVTTGSEEVVEQITKQLNKLIEVVKVVDLSEAPHIERELMLIKVRATGRDREEMK
                     RMADIFRGRIIDVTDSTYTIELTGTGDKLDAFIHALDKTAILETVRTGASGIGRGERV
                     LRV"
     gene            27942..28958
                     /gene="ilvC"
                     /locus_tag="VKZ48_00365"
     CDS             27942..28958
                     /gene="ilvC"
                     /locus_tag="VKZ48_00365"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HLU76335.1"
                     /translation="MKVFYDKDADLSLIKGKKVTIVGYGSQGHAHALNLKDSGVDVTV
                     GLRKNGASWGKATNAGLNVAEIAEAVKGADVVMVLLPDEQIANVYKREIEPNIKQNAS
                     LAFAHGFNIHYGQVQPRADLDVWMVAPKAPGHTVRSTYTQGGGVPQLIAVYADNTGKA
                     RDLALSYAAANGGGRAGIIETTFKEETETDLFGEQAVLCGGAVELIKAGFETLVEAGY
                     APEMAYFECLHELKLIVDLIYEGGIGNMNYSISNNAEYGEYVTGPKIVTEETRQAMRQ
                     ALKDIQTGEYAKSFILENHAGAPTLLSRRRLTAEHPIEQVGEQLRAMMPWIKKNKLVD
                     QSKN"
     gene            29010..29660
                     /locus_tag="VKZ48_00370"
     CDS             29010..29660
                     /locus_tag="VKZ48_00370"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HLU76336.1"
                     /translation="MASPYPHPILAREGWLYSFLAVAAAVGVCVFAGFWWSVPFWIVA
                     IFVLQFFRDPAREVPGDSRTVVCPADGRVLMVVPAEDPYLNRPAIKVSIFMNVFNVHS
                     NRSPVDGEVVDAWYTPGSFFNADLDKASLENERNALHLRTDSGVDVTCVQIAGLIARR
                     VLCYVKPGDRLVRGQRFGFIRFGSRVDVYLPPGTRVKVELGQKVHASSTVLAELEA"
     gene            29657..30418
                     /gene="pssA"
                     /locus_tag="VKZ48_00375"
     CDS             29657..30418
                     /gene="pssA"
                     /locus_tag="VKZ48_00375"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HLU76337.1"
                     /translation="MSEYQTQTRDNLRGTLRHRGIYLLPNLFTSGVLFSGFYAIVQAM
                     NGRYELAATAVFVAMVLDGLDGRIARLTRTQSEFGAQYDSLADVVAFGAAPALIMYVW
                     ALQPLGRIGWIVSFVYCVCVALRLARFNTNIGVVDKKWFQGLPSPSGAALVAGLVWIM
                     NDLGFDPVGFGRALAVIFTLFAGITMVSSVPFYSFKTINFRRAVPFWAILLLILVLVI
                     VTSHPPLVLFIFFVAYSLSGYIMWLWKFRHKRSPF"
     gene            30429..>31133
                     /locus_tag="VKZ48_00380"
     CDS             30429..>31133
                     /locus_tag="VKZ48_00380"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008341793.1"
                     /note="catalyzes the formation of 2-isopropylmalate from
                     acetyl-CoA and 2-oxoisovalerate in leucine biosynthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HLU76338.1"
                     /translation="MSADRLIIFDTTLRDGEQSPGASMTREEKVRIAKQLERLRVDVI
                     EAGFPAASNGDFEAVRAVANAIKDSRVAGLCRANERDIDRGAEALKEAASARIHTFIA
                     TSALHMEKKLRMSPEQVLEQAQVAVKYARKYCDDVEFSPEDASRSDPEFLCRVIEAVI
                     KAGARTINIADTVGYAVPQQYGEFIRSLRERIPNSDQAIWSVHCHNDLGMAVANSLAG
                     VHIGGARQVECTINGLG"
BASE COUNT         5491 a         9647 c        10039 g         5956 t
ORIGIN      
        1 accgtcagtt cctccatcgc tgcaatatcg gcagcgaatc gatgtcgcca gaggagcagg
       61 cgcgtgtggc cgacatcggc aagcgctatc gctggcgcga tgtgacggga cacacggtgc
      121 attttctcac cgacgacgaa atcaagaacc tcagtatccc gcacggaacg ctcacggacg
      181 acgagcgcga aatcatcaac tatcacatcg tctcgaccat caacatgctc gaatcgctgc
      241 cctggccgaa gcacctgcgc aacgtaccgg aattcgccgg agggcaccat gagcgtatgg
      301 acgggcgggg ctatccgcgc gggttgaaag gtgaccagat gtcggtgcag gcgcgggtca
      361 tggcgattgc ggatatcttc gaagcattga ccgcaaaaga tcgtccgtat aagcgcggca
      421 agacgctgtc cgagtcgctg cagatactcg gcaaattcaa actcaacggc catatcgatc
      481 cggacctgtt cgacatcttc atccgcgaaa aggtgtacct gcgctacgcc gaacagttca
      541 tcgaccgcga gcagatcgac gaggtggatg agtcgcgaat ccccggctac gacggctgaa
      601 ctgtcgacgc gctaacaacc tgtttgcaat tcaacagtta cgcaaaatgt gcggcgactg
      661 acgtacagcg tcacatcacc cgaggctgcc ggagattgaa gtgtgaagct tttcacgcca
      721 tgcaatcgtc ataatttctg tcgccgaaaa atcaggttgt ttaataggta tttacagcaa
      781 cgcacgggaa ggctcgtcaa aactggtcta agccttgctc aaaaggcttc gcgcgagacg
      841 attaccggtc tcgtctccac caacaactcg aggagagtta gaaaatgaaa gcacaaaagg
      901 gctttacgct tatcgaactg atgatcgtgg tcgcgatcat cggtattctg gcggccgtcg
      961 cgctgccggc gtatcaggac tacaccaagc gcgcgaagat gtccgaggtg attctcgcgg
     1021 cgagcgcatg ccgaacgacc attacggaag tctatcagtc gggcgatacc ggtcctggcg
     1081 ccaacaactg ggggtgtgag ccttcgagcc cgccgtccaa gtacgtgcaa agcattgcta
     1141 ccgacgaaaa tggtgtcgtg actgtgactg caacgggctt caacgataac caaatcgata
     1201 accgtgtagt gacactggtc ccttatgtta ataacaatgc ccctatgacg gccgctaacg
     1261 acatgggcaa gggcgtcttc aaatgggtat gtggcgacac ggggacaacg attgctgcca
     1321 agtatctgcc cggatcctgc aagggcgtct aatcagttca ccgtcgtaac tgagaaaagg
     1381 tgcctcgcgg caccttttct ttttctgtaa cttggctgga gcaaaaagca tcggttccaa
     1441 aaaaattgct cgagaatctt aattagattc gttcgtgcgt acatgcttac gatcccaatc
     1501 ctttgtgcat tgtcggcggt tgtctatgcg gcctggatgc gggcgcaaaa gccggtctat
     1561 ctgttcgcgc tggtcgtgtt cctgctgtcc accatcctct cattggtcta cttcgtctcg
     1621 gatgacatta ccggagaagg gattaccgag gcgacgctgt ttcatctgat gtacggcttt
     1681 gacgggatcg accttgccgg tttttcggta tacgttgcgc tttccattat ttgcttggtt
     1741 gcgatacccg gcatcgggta cttcggctgg aagtcctgta cccgtcagac gaggcgaggc
     1801 ccatcctatc ggccgtcagt gatcgtccat ctgaccgtca ttggtgcgtt gagtgtcttt
     1861 gcggtcggcg ttcatccggc aacgctcgat accatgcagg tcgtggagaa gttcaacgac
     1921 gcccgctaca acgggctgtt gagtgaggaa ttgcgcgagc tcgatggcat tgcgcccccg
     1981 aaacggggaa agtccctggt ctacatctat gcggaaagca tggagcggac gttcttcgac
     2041 aaaagaatct ttccgggtct tatacaggaa ctggcaaagc tcgaagagtc gtcgctttcg
     2101 ataaggggca tcggccaagc accgctgacc gactggacga tcgcgggaat ggtcgcaagc
     2161 caatgcggca taccgttggc aacattccgc tccaatagaa acgatctggg cgatttgaaa
     2221 tccttcatgc ccggagcgac ctgcctcggt gacatattga atcaggccgg ttatcgattg
     2281 gcgtatatgg gcggggcaga tctcagtttc gcgggcaaag gccagtttta tcgcgaccac
     2341 agcttttccg atgtaaccgg tctgaaagag ctccagaaca ggttgggtga agacatcccg
     2401 ctttcaaaat ggggcgttta cgacgatcga ctcttcgaac aagcggtaga gaagttccgt
     2461 gagttgtcga agaccggtga gccgtttgct ctcatcatgc ttacgttgga cacgcatgcg
     2521 cccaccggcc atgaaacgcc gacctgcgct cgtgacccga atctcggata ccgcgatgga
     2581 tcatcaaaga tgctgaacgc ggtgaaatgt tcagatcgat tgattgcgcg tttcgtggag
     2641 caaatcgagg cggaagcggg taacgacgtc gtaatcgtcg ttgcgtcgga tcactggcag
     2701 atgcgtagtg atgccgcgga accattatcg cgcagccctg tgcgtgaaaa tcttttcctg
     2761 gtgcgtggcg ccggcatcga gccgggggtc atcagcaagc cttctacgac catggatatc
     2821 gcgcccacgg ttctttcgct gcttggctgg gatatttcta cgtttgcgct tggaagaaac
     2881 ctgttaagag atgagcccac actggtcgag aaattcggcc gcgctcagta ctacgacatg
     2941 gtgaagtcct ggcggatgaa tttatggagg gtctgggaag ggcagtctca gtccgattca
     3001 gccgctcccg cgctcgaaga cggtgcggct tgacgcggtg cttcaaaagc ggcccgaagg
     3061 gagatcttcg gcgcctgcga gtgtgctccg ttcagacttg tgcggctagt ccattattac
     3121 ggctcggcct tgagcgcgca gctcgtcggc cctccgttgt agatatcgct gaaacgacgg
     3181 ctccttcagg tacgcacccg agctgatgta atcgaatgcc gaggccaggt ggaaggggcc
     3241 gacataggct tcgaggcgaa agaccgcttt gccgtactga tcgaagaaca ggacgctggg
     3301 cgtgtaggtg atccgctgcg ttgccagcca gtcggcagcg gtggtgctct ggccgtccgg
     3361 ggtggtgatg gggcttcgct catcgagtgc aaatcgcgcg atatcgagct tgtcgatctg
     3421 ctcccggact tgcggattgg ggaaaccttt ttcatgcagt tcggcacatg tctcgcatga
     3481 aggggcttcg accaacacag ccagcggctt gccttcgggc ttgcggcgca ggtcgtaagg
     3541 cggcttgatg aagaacggct gatcaacgaa cccactcttc atttttgctt tcggcttggc
     3601 cgttgcctgc tgcagatgct cggcaaagcc gattttgctt tccaggtgtt cgcctgcata
     3661 gcgcagcgcc gccatgaagc gcgccggctc gtagtagccg ttgatgcggg cgatgatgtc
     3721 gccctcctcg ttcaggaaga gcagggtcgg cgtgaactgg acgtcgagcg cctttgccag
     3781 atccttttcc gcgcgcgcta caccatcgag ccagaccgtc tcccggtcgc cccacatgtt
     3841 gagctcgatg gcgatgaagt gctcgcgggt gtaggcgacg atgtccggct gcttgaagtt
     3901 cacctgcatc agccgggcgc aatacggaca gccgtcctgg ccgatgtaga gcatgacgcg
     3961 cttgccttgc cgccgagcgt cgtctacgtc ttcacggaag tcgagaaagc tgggcgcaaa
     4021 ccatccaggt atctggatcg cgtgtggcga ggcgttttgc gcgcgtaccg cgccgctgcc
     4081 taccactgca aggatggcaa gcgcggcgag caaggcgcga aagagagtgg aaaggtgcat
     4141 cgaacactcc ggcggtcgcg cctgccgggc cgatgcgcag cgcaagcggc ccggggtcga
     4201 accgttactg attgacgacg gactccggat cgagcagcag cgccgtcagg tccttcatct
     4261 gttgctcggt cagcacaccg tgataaccga agcgtggcat gttggagcag ggtacgtaag
     4321 cctgggaatc gtagagcttg ccgtacacgt acttcatgtt ggcttcggtc gggccgccgc
     4381 gcagtttgcc gaactgatag agcgaggggc cgatggtgcc gtaggacagt tccgtgcccg
     4441 agacgcgatg gcagttgtag cagcctccgc cgtttaccgt gtccggcttg tcccgccacg
     4501 tggcaccgcg tccgctcttg gcgatcttgt cgccgttttt ccagtcgccc atgtacttgc
     4561 cgtcggccgg gtacttgatc agcgccattt gcgccgcttg gagttcccgt gcgatgtcgg
     4621 cgggcgggtc gccgcgatac ttggtgcaga ccgccatggc ctgatcctga tccagccggt
     4681 cgacggtggc gatacccgtg ctcttgaaat cctgcttcat cagggcggtg gcttttttga
     4741 gggcgacggg gtcgctgctc ttgtcgccgg cgtggacggc gcttgcgcca agcgagaggg
     4801 caagcgtggc cagcatcatg gtttttttca tggtcgctct ccttagcgct tgatggccgg
     4861 gccgtcgtac ttggcgccgt tggcgttgac cgcgaggaag gtggtcaagg cgatggttgc
     4921 gtcggagatg tacttgggct cggggaagcg ttgctgccga aagcagtccc acaggcgcca
     4981 ctgcatggtg cgcaactcgc cctgcgacac gcggtatgcc ggccaggtgg tgtacgcgaa
     5041 ttgcgcccct ttcttctcgg tgagattggg caggtcctgc aggcggatgc gcttgccgtc
     5101 ctcgccgtgg caggtggcgc aggcaaaatc ataagggccg gcgcggtaat agaagatctc
     5161 cttgccgatg gcgtaggctt ccttctcctt cgggtgcttt tgcggcacgg ccatcggcag
     5221 gccgcgcgac tgtccgacca cataggcggc cagggcctcc atgtccgcct ggtaggagat
     5281 gctgccgtac ggttttttcg tcacctcttc gcgatcgaag ccctgcagcg tggtcatgca
     5341 ggtgaccaga cgggattcga ggtcctcgac tttgtcggta tcggggaaat agcgcggcaa
     5401 ctgcgcgtag gcgccctgca ccttgcccgg ccccagaccg agatcgcact tttccagcga
     5461 ggcgtttttc gggccgcgcg gcgtcgtcca gagttcttcg ccccgaagct cgtgcaattc
     5521 ggccgggttg ccgtcggcga gcatctcccg gtacatctcg attgccgcgg ctgtgctgtc
     5581 cttgtcttcc tgcgcgagca gcgcacttgg cacgagcagc gcaaaggcgc aaatgccttg
     5641 cgccaggctt cgtctcagcg tcgccatagc gttccctcct ggttgtttgt gtttcaagcg
     5701 ggcggcgggc ccgcctgttc ggaatgagcg cttacgcgcc gcccagctcg acggtgtcgg
     5761 tgcgcttgtc gccgcgattg tcttcccagg tgatggaaag cttgtcgccg gccttggcgc
     5821 ctttcacttt gaaggcgaaa accgggttgc gcgacaccgc ctggctgatg gcggcttcga
     5881 acaccggctt gccgttcaat tcggcccgga tatccttgat gaagtgcatg ggcacgagtt
     5941 ctccggcagc gttcttgcgc tgcccggttt ccatcgggtg cgacatcagc acgcgcacgt
     6001 tcgccacgtc gccttcgagc tttgcgcgga ttttcattgg gttggccatg attgctccgt
     6061 aatgttgcgc gaccggctta gccgccgcat ccgccgatgg tgactttgac ttccttgacc
     6121 gcctggtagt gcttgccgtt ggctgtcgcg atgacgcgca cattcgacga ctcgcccatt
     6181 ttgacgcgca catgcacgta gggcagggcg cccttggtga agtcgaagct gcccacgtag
     6241 gggaaggggt tcttgtcgat cagcacggtg atgcgctccg tgcccgggat gtcgctggtg
     6301 atgtcgatcg gcacgacggc gccgttctcg gcgatatcgg gtgccttgat ggtgatgtcg
     6361 ccgctgtcga ccggattctg ggcgccgagt ttctgcaagg cgtcggcgac gtttttggca
     6421 tggaacgcat cctggttcca ctcgatggcg agtgcatcgg caggacgcag cagcccggtc
     6481 gcgaagccgg ccacgagcat cgacgacagc ccggcgcttt tcagcgcgac gcgacggttt
     6541 cgattcatta cgagactcct tgtctgggat cgccgcgagg cgatcgtcgg ccgacggtgc
     6601 cggctcagtt cggaaagacg cagagggtac tgaaaatatg cccagtcgtg caaggcacga
     6661 caggcatatt acgaatcgag atttgctttg aactacaagt aattacgtaa gtgccggttc
     6721 aggcgtccgg gtggcggaaa tggccggatt tgccgccctg cttttccagc agtttgacgc
     6781 ctacgatccg cataccgcgg tcgaccgcct tgcacatgtc gtagacggtg agcaggccga
     6841 cgctgacggc cgtcagcgct tccatctcga cgcccgtccg cccgcgggtc tcgacggtga
     6901 cctgcagcgt gacggtgctg gcttctgcgt cgaccgtgaa ctccgtcccg attttggtca
     6961 gcgcgagcgg atggcataag gggatcagat cggcggtgcg cttggcgccc tggatggcgg
     7021 cgatgcgcgc caccccgagc acatcgcctt tcttcgcgct gccggagcga atcagttcca
     7081 gagtctcggg gcgcatatgg agggtgccgc tcgcccgcgc gatccgatgc gtctcggctt
     7141 tatcgccgac gtccaccata tgggcttggc cgtcggcatc gaaatgggta aggctcatcg
     7201 tggggagacc gtgtttggaa tccgccacat tatgccccac tcaggcgctc ctccccccgc
     7261 gccgatgcgg caccgcaggc ggaaccaagg ccgcggcccg gctatcataa gcgcgtgcga
     7321 ttcctactca gcctactgct ctccacggcg ctcgcgctgc cgccgttcgt ccacgctcaa
     7381 aacctgcccg acctcggcga gtcctcgcag gcggagttct cgccgcacgc cgagcgcgaa
     7441 ctggggcagt cgatcatgag ccggatacgg cgcgaccccg cctatatcga cgatccggaa
     7501 gtggccgcgt acatcagtgg catcggtcag cgcctggcgg ccgccacgcc cgagacggcc
     7561 ggcatcgatt tccacttctt cgccgttcgc gatcccaata tcaacgcctt cgcgctgccc
     7621 ggcggctaca tcggcgtgca ttcgggactg gtgaccgcgg cgatgacgga gtccgaattc
     7681 gccagtgtgc tggcgcacga aatcgcgcac gtgtcgcaac ggcatattgc gcggcaactg
     7741 caggcccaga gccagatcgg cgcactgtcg atgattggca tggtcatcgc gatgctcgcc
     7801 gccagttcca atccgcaggt cgccgaggcc gcagccgtcg ccgggtcggc ggcgccggtt
     7861 gccgcctttc tgaactacag ccgggaattc gagcgcgagg ccgaccgtct cggctatcag
     7921 atcctgcgag cggccgagtt cgacgtccac gccatgccga gctttttcga acgcctgcag
     7981 cgcagcacgc ggctttacga aaacaacgcg cccggctatt tgcgcaccca tccgctcacc
     8041 accgagcgga ttgccgacat gcagcaccgc acgcgcgagg aacggccggc gcgcatgcag
     8101 ccggacagcc gcaccttcca gatcgtgcgc gccaagctgc gtgcggagca aggcgatgcc
     8161 tccgacgcgg ctgccttctt ccgtgaggcg ctggcggacc ggcgctatgc cgaccggggt
     8221 gcggcgcgct acggctatgt ggtcgcgctg ttgcgcatgg gcgatgtggc gaccgcgcgc
     8281 gcgcagtggc tcgagttgcg caaggccgcc gattcgcatc cgatgtacga aaccgtcggc
     8341 gcacgcgtct atctggctgc cggcgaaccg gaagaggcgg tccgggtgct gcaacgcgcg
     8401 gtgaagaagc atccgcacaa cgacctcgtt cgcgtggcct acgccgacgt actgcagcag
     8461 gcgcaccggc accaggaggc ggtttccctg ttgagcacac tgcgatccga gcgcccgggc
     8521 gaaccgcgct tttatcagat gctcgccaag agctatgccg ggctggggca acgcgtggaa
     8581 cagcaccgcg cgctggcgga gtactactac ttgaccggca gcctgccggg cgccatccag
     8641 cagttggaac tcgcccagag cgccaaggat gcggatttct atacgctgtc ggcggtggat
     8701 gcgcgactgc gccagttgcg cgcgcagcag ttgcaggaaa tgaaggagcg tcagcgctag
     8761 tcgaagggtt cagcgctccc tttcaggatt tccgcgaagg cgcatccaga tacgcttcgt
     8821 acagcaggtc gaaggtcatt tcgcactcct gcagcaagtc gtggtcgttc gccgcgcggc
     8881 gacgcgcacc gtcgagcagc gtttccacgc cgaccgcctc ggccagttga cgatcgccga
     8941 catccagcgc gcgcaccagt atgccgatgc gcgccagcgc cggttcgttc tgccggccgc
     9001 aatacgccag cagttcctcg tacgtgacgc gcgcccggct gttctggaag cttgcacctt
     9061 cgtagccata ggtcaccgca tcgagcggcg actcatcgtc gtgatcgagc catagcatgg
     9121 tcgcttccgg atcgatgaag cggcggatca gccaggcgct ggccaggcgg tccgcgtaca
     9181 gcggccggcg cgtcgcccag acccgctgca gatagcgctt gggccgtgcg gtcgagggct
     9241 gggtaacgtc cgggaagatc agccggtgca cccgttcctc cagcgcaccg agtcgttctt
     9301 cgacctgcac cgcttgcggc gagccataga aatcgagcga gcggatgcgc gtcaactcgc
     9361 gccgctgctt ggccagcacg cggcccagcg cggccgggtc ggccatgccg atactggatt
     9421 cgaggctgcc cagcgtctgc agcagttgcc cgtattgcgc gctgcggtcg aacagcgccc
     9481 ggatgcgctt ggtctgcacg ggatcgaggc tttccaccgc gagcacttca cagctcgcct
     9541 cggcaccgat cagatagttg gccaggcgct gcaggctgcg cgcatggtcg ggggtgtccg
     9601 gcaagaggta gacgcctttg cgcaacgccg ccgcgcccag cgtctccagc gtgcgcagca
     9661 ttgccatgcg cgtaccggtc gcatcctgcg gcaggtgcac gatgagcgcg agccatcggg
     9721 aagaggacgg ttgaagtggg gtcggcacgg caatgacaat agcataaaac tccgcgagca
     9781 acgacggccg gcccgctcct ccgtcagtgt agctttgttg aagcgcggcg gcgaacccgc
     9841 tataaagcgg caacacgttt tacaagcagg agcaccagca tggatttcga caaggacctg
     9901 gacgcgcgcg gactcaactg cccgctgccc atcctgcgca ccaagaaggc gctcgcggaa
     9961 atgtccgccg ggcaggtatt gcgcatcctc gccaccgacc cgggttcagt gaaggatttc
    10021 gaggctttcg ccaagcagac cggcaacacg ctgctcgagc atggcgaggc gaacaacgag
    10081 ttcaccttct acatgcggaa gaagtaacgg ggcgtccggc cttcagttgt ccgctttgtt
    10141 caagtcaggg cgggcatggg cgcccgcgcg gttcggcgta tcggttgcgc cggacttttc
    10201 cgggttcggc agcggcaaca ccatctgcac tacgaccccg cggcggtctt cacggttgcg
    10261 cgccatggcg cgcccgtgat gaaactcggc aatcagccgg acgatgaaca gtcccaaccc
    10321 caggtggggc gtattgcgcc gctccgtggc ggcatcgcgc cgcatgctga tcatcgactc
    10381 gaacagccgt ccctgcatcg ccgccggcag ttccgggcct tcgttggcca cggcaagacg
    10441 cgcttcgccg tcggtaagcg cgacgctcac ttcgatcggt cgtcccgctt cgctgaaatc
    10501 catcgcgttc tcgaccagct tgtccagcgc ctgcgcgatg agatcgggcg aaccgtcgat
    10561 ttcgatgcgg cgttcgggca ggtgcaggtc gaaccggaat gccgggaagg cgcccgcata
    10621 gccggcgacg cagccgctca ccacgcgccc caggtcgaag cgctcgcggt cggcatggcg
    10681 cagcatctgc tccagccgcg tcgcctctgc gatgcgggtg aggatcaggt tgagccgctt
    10741 cacgccctct tccgcgcggg tgatatagac gcgtgcgctg tccggcacgg tctcgctttt
    10801 caggttctcc agcgatgagc tgaccaccgc gaccggtgtg cgcagttcgt gcgacaggcg
    10861 gtccgccatg cgttccagat aggtggtgta gtcggcgagc cgttcgagca tcgccgagaa
    10921 actgcgcgac aggtcgccga tctcgtcgcc gtcgcttgct gccggcaggc cgtgcatcac
    10981 ccgcccttgc gcatcgatgg cgtgttcggc gctgtcgcgc aggcgctgca ggcggcgtga
    11041 caggctggag gcgaaaaaca gcagggtgcc cgcaccgagc aggaagaccg cgagcgtgac
    11101 ggcgatcagt tgctcgatgg cgcggttgcg cagactgagc accgcgttgg tcgtttcttc
    11161 caccaccacc gcgcccacga cttcgtcgcc gacccagatg ggatgcgcgg ccgacaggat
    11221 gacggcgcgc tgatcggtgg tctgccgcca gcgcgatgcg ggcacgccgc tcagcgcgga
    11281 atagacttcg cgcccgccgg acagcacctg ttcggggagg gcgtcgtcga agtcctcgcg
    11341 cggccgttcc agcagcagtt tgtagagcgg tcgcagcacc ggttcggcga tgcgttccca
    11401 cagaccgggt tcgcgttccg tcgcctcgag cgaatcgcgt cgcagcccgc cttcgagcgc
    11461 gagcaaccgg tgctgccgat cgatcaccca gatgcgcgaa ctggaacgcc gcaggctttt
    11521 gacgatctgt tcgatctcgc cggtggacgg cgccgcggcg agggacgcat cgtcgccggc
    11581 tgcgacggca cggcgttcca gcagctccgg gcggtcgtgc agcgtcgtcg ccaccgcgcg
    11641 cgccgtggcg agcagcacct gctcctggcc cgtgcgcaat acgttttcca tttcgcgcac
    11701 gaagctgtag ccgaccagcg ggatcgcaag caggacgagc gaaacgagaa gcagcttgct
    11761 gcggatgctg agcgtcagtc ggggccagcg catggactac tgcgcagtcc agcggtagcc
    11821 catgccgtac accgtatcga tggcgtcgaa gtccggatcg acggcaagga acttcttgcg
    11881 catgcgcttg atgtgcgagg tgacggtggt gtcgtcgacc acgagattcg cttcggacat
    11941 cagctgttcg cggcttttga cgtgaccggg atatttcacc agggcgttca ccatccagaa
    12001 ttccgtcagc gtgaggtcca cggggcgtcc gcgccattgt gccgtcatgc gtttcaggtc
    12061 cagtcgcaaa tggccgcgct ccaccagttc ctcggcgtga tccggcgtgg agagcacatc
    12121 gacccggcgg aacagcgccg cgatgcgcgc gagcagatgc ggcatgctga cgtccttggt
    12181 gaggtagtcg tccgccccca ggcgcaaccc tgcgaccaga tcgaaatcgc tgtcgcgcgc
    12241 ggtcaggaaa atgatcggca gcgtctcgga tagcgcgcgc agctcgcgca cgagcgtgaa
    12301 gccgccgtcc acttccgcgc ccaggccgat gtcgacaatg gccagatcgg gcaggcgcgt
    12361 cttgaacgcg gccaaggcct gctggcgatc ggggtaggtg ctgagttcgt agccctgtcg
    12421 acgcagcgcg tcggcgacgt tctcccggat cgcggggtca tcctcgacga tggcgatacg
    12481 gcggctcatg aggtcctcct tgcttccatc cgcgcaatat aaccgacccg accggcggtg
    12541 ccgccggtcg catgcagaag ccattatctc gccatgttcc ggcatcgctg cgacccgatg
    12601 gcgcatcgtc ctgcccgcac cgcccgctgc aatgaagcct cgcgctgaaa aaggaacaac
    12661 aaatgacggc tcgcagcgat cgctacccgc gccatgccgg caggctggcc gattttctgg
    12721 aaacgctcag tctggtgctg gtcaccggcg cactgacggg cgtcggcttc attgtggccg
    12781 ccgtgctgct cacctgggcg accgccgcgt ttgcgggcga gtccgccgag gccgcaccgg
    12841 ccggggtgct gacgctgcgc accgcagacg gcaagtccgt cgcgagcgcg ccgcgtctgg
    12901 cgaccgaagt gcgcatgcgc atcaccggta tggttgcgcg cgtgcgtgtg gtgcagcggt
    12961 tcgagaatcc ttatgacgac tggtacgaag gcgtctatgt ctttccactg cccgacaacg
    13021 ccgccgtcga caccctgcag atgcgcgtgg gcgatcgcct cgtgcaaggc gtgatcaagg
    13081 aacgccaagc ggcaaagcga agttacgagc aggcgaaggc acgcggttcg cgggcggcat
    13141 tgctggagca ggagcgccct aatattttca ccaccagcgt ggccaacatc ggtccgcgcg
    13201 aaacggtctc ggtggagatc gaataccagc aggcggtgcg ctacgccgac ggacgcttca
    13261 gtctgcgctt tccgctggtg gtggcgccgc gctacatccc gggcctgccg ctggacgcat
    13321 cgcccggcgg cagcggttgg gcgcgcgaca cgacgcaggt gcccgatgcg gcgcgcatca
    13381 cgccgccggt ccttcatccg cgcaaactgc cggacgacgg cgtcctcaat ccggtcgata
    13441 tcgcgataga actcgatgcg ggcatcccgc tggcggacgt ggcgagcagt tcgcacgact
    13501 tgcgcgtcga gcgtcggagc gagcgcgcat ttcgcattac gccggcgcga gcgcatatcg
    13561 ctgccgaccg tgacttcacg ctcgactgga taccgaagca gggcgcggca ccctacgcgg
    13621 cattcttccg tgaacggcat ggcgatgccg attacggact gctgatgatc atgccccccg
    13681 gtggcgatgc aacgcgtacg cgtctgccgc gcgaagtcgt gttcgtcatc gatacctcgg
    13741 gttcgatggc cggcgcatcc atcgagcagg cgcgcgaagc gctgatgctg gcgctcaggc
    13801 ggttgacgcc gacggaccgc ttcaatgtca tcgaattcaa ttcggtgacg cgcgccttcg
    13861 ccgcggaagc attacccgcg acgccggcac gcatccggcg ggcactcgcc tgggtggcgg
    13921 cgctgcaggc gcagggcggc accgaaatgg cggaggcttt gcgcaccgct ttgacggctt
    13981 cgcgcgatcc gcacctgttg cgtcaggtga tctttctgac cgacgccggc gtgggcaacg
    14041 aaaccgagtt gtatgcgctg atccgtgagc gcctcggcga ttcgcgcctg tttaccgtgg
    14101 gcatcggttc ggcgccgaac ggctacttca tgcgcaaggc cgcggaagtg gggcgcggca
    14161 cctataccta catcggtcgc acggatgagg tggcgcggaa aatgggcgag ttgtacgcca
    14221 aactcgaatc gcccgcgatg aagaatctgc gcgtgcagtg gccgtccggt gtggcggtcg
    14281 aaacctaccc gacgcgactg cccgatctgt atcacggcga gccgctgctg ctgaccgtgc
    14341 gcgcaccgga actgtcgggt ccgttgcgtg tggaaggtga acagggcgcg ttgcgctggt
    14401 cgcagacgct gccgttacgg catggggtgc cgaatgcggg catcgccaga ctatgggcgc
    14461 gcgcgaagat cgatgcgctg atggacggcg tgcacgaagg gcgcagtctc gaggcggtaa
    14521 agcgggacgt gatcgacgtt gcgctcgatc acgatctggt gagccgctac accagtctgg
    14581 tcgccgtcga gcagatgccg gtgcgaccgg cggacgccgc gctgaagccg ggcgtcgtgc
    14641 cgaaccagtt gcctgaggga tgggtgcatg aggcggtgtt cggtccgttg ccgcagacgg
    14701 cgacgccggc agcggtgcat cggctggcag gcgccattgc gctgctgctg gctgcgttgc
    14761 tgtatttcct gtcgtggagg cgggcgcatg cgtatcccta aatttcacgc gctccaatgg
    14821 gcagcggcag tgctgcttgc cgtcgcacta tggcagttcg ggcatgcagg ttacatccac
    14881 gccaaagcag tgttggcgca gtccctgatc cacaacgcat ggatcaggac gcaggcgacg
    14941 ggcgagccgg tcaagccctg gccctgggcg gacacgtggc cggtcgcgcg cctgcaggtg
    15001 ccgcgactgg gcgtggagct ttatgtgctt gcgggcgcat ccggccgcac gctcgctttc
    15061 gggccgggtc atttgtacgg cacggctgcg ccgggcgaga cgggcaacag tgtgatcgcc
    15121 gcgcatcgcg atacgcactt cgcctttttg cgtgatctgc gtgccggcga cgaggtgatc
    15181 gtggaaacgc tcaccggccg cgtgacgcgc tacgcggtgt ccggcgcacg tatcgtcgac
    15241 gaatacgatc gcgacgtgct ggagcaggac gctgtcgcgg cgcgcctgac gctggtgacc
    15301 tgctggccct tcgacgcggt cgtgccgaac gggccgctgc gttatgtggt gagtgctcag
    15361 gtgcgggcgg gctgacccga tgcatcctgt gcggcgcgtg cgcggcggat cgattcgcgc
    15421 cacgtcggca ggctgtaggc gaagcaggcg gcggcgatcg cgatgccgct gccgcagagg
    15481 aaaaacgtcc attgcacgcc gatctgttcg gccagcgcgc cgctcaggaa atgtccgatg
    15541 ggcgacatgc cgaagaaggc gaggacatag agtccgatga cgcggccgcg cttgtcctct
    15601 tcggccacgg tctgcacgac cgtgttgcaa ctggcgaccg tcgcgatgag gccgaagccg
    15661 aacagcgcga gcagcggcag tcccagccag aggctgttca tccacgcaaa gcccgccatg
    15721 cccacgccgc tcagcgcgca cgtgactgcg attacgcttc ccagaccgcg caggcccggg
    15781 cggcgcatga gatgcgctgc gcccagcagg gcaccgacgc ccaccgcacc gatcagccag
    15841 cccagcgtgc gcgagtcgcc gccgtatatc tccgtggcga aaatcggcat gagcggctgc
    15901 cagggcgagg aaccgatgga cagcacggcg agcagcagca acaggcgtcg tatgcccagc
    15961 aaaccgaacg cgtaacgcac gccctcttga agatcgtgca gccaatgcgc gttgcgtgcg
    16021 gtccatgcgc ggggcagcag gcgcatgtgc gtgtaggcgt agagcgtggc gacgaaagac
    16081 agtccgttga gcagaaaaca ccatgcttcg cccaacaccg ccagtgcgac gccggcgatc
    16141 gccggcccga cgaagcgcgc gccgttgaac aacagcgaac tgagtgcgat ggcgttgggc
    16201 agatcgcttt tgtcgccgat catttccagc acgaaggcct ggcgcgtcgg cgtatcgaag
    16261 gcattgatca ggccgagaag aaaggcaagc agcatgatgt gctcgacccg gacggtgccg
    16321 ctgaaggtca gcatggcgag cgtgagcgcc tggaatgcgg ccaggctctg cgtgatcatc
    16381 agcatcctgc gccggtcgac acgatcggac cacacgccgg ccagaggcga gagcagcagc
    16441 atcggcaatt gctgcaggcc ggtggtgacg cccagcagca gggcggagcc ggtcagtcga
    16501 tagaccagcc agacggtcgc aacgatctgc agccaggtgc cgagcagcga aatgaactgg
    16561 ccgaagaaaa acagccggaa gttcggatgg cgcagcgagc gtaacgcatg gttcatggct
    16621 gcctgtcggt gcatgcggcg tgccgccgcg cgtcgggacg aacccaagcc aaaagcaaac
    16681 cgttcgtggt gagcggacgc gtcggggtcc ggtcgaacca tgaacggcca tacgacggtt
    16741 ttgcgactcc ccgttcaccc ttcgactcgc gcctgcggcg cggctcaggg cgaacggttt
    16801 gcttcatgcc ttcagcttgt ccagttgcgg ccgcagcttc tccagcgtgg cgctgaagtt
    16861 cgccagccgg tccttttcct gtgcgactac gttggcgggg gcccgctcga cgaaactggc
    16921 gttgctcagc ttggcttgcg ccttggcgat ttccgcttcg agccgcgcga cttccttgct
    16981 cagccgttca cgttcggcgt cggcgtccac ttccacctcg agcatgaggc gtgtggtgcc
    17041 aaccaccgac accggcgcat cggcttcggg cagcggattg accacgctcg cctccgacaa
    17101 gcgtgcgagc aactgcatgt acgggaaata gcgcgtcagc gtggtcgcat ccccttcggc
    17161 cagcagcggc acacgctgcg agggcgagat gttcatttca ccgcgcaggt tacggcaggc
    17221 gtccgtgagc gctttcaact cggcgacctg ttgctcggct tcctcatcca ggcgctcgct
    17281 gcgcggacgt ggataggcgg ccagcatgat gctgtcggcg ggcagcttgc cgtcggcagt
    17341 cgtgagcggc gcaaccttct gccacaactc ttcggtgatg aagggaatga ccggatgcgc
    17401 caagcgcagc accgcttcca gcacgcgcac cagcgtgcgg cgcgtggcgc gctgttgagc
    17461 gtcgttgccc gactgcaatt gcaccttggc cagttccaca taccagtcgc agtactcgtc
    17521 ccacacgaat tcgtagatgg cgcgcgagac ctggtcgaaa cggtagtcgc ggaaggcgtc
    17581 atgcacttgc gactcggcgc gttgcaggcg gctgatgatc cagcgatcga cgaaggacag
    17641 ttcgagcgac agcgattcgt cctgaccgca gtccttgcct tcggtgttca tcagcacgaa
    17701 gcgcgtggcg ttccacagct tgttgcagaa gttgcgataa ccctcgcagc gcgcgagatc
    17761 gaacttgatg tcgcgaccgt gcgtggcgag gctcgcgaag gtgaagcgca gcgcgtcggt
    17821 gccgtacgac ggaatgccgt tggggaactg ccggcgcgtc gctttctcga tctgttccgc
    17881 ctgtttggga ttcatcaggc cgaaggtgcg cttctcgatc agcgcattga gttcaatgcc
    17941 gtcgatgatg tcgagcgggt cgaggatgtt gcccttcgac ttcgacatct tctggccttc
    18001 ggcgtcgcga acgaggcccg tcacatagac ttcgcggaac ggcaccttgc cggtgatgtg
    18061 cttggtcatc atgaccatgc gcgccaccca gaagaaaatg atgtcgaagc cggtgaccag
    18121 caccgacgtc ggcagatagg tgtcgagcgc agggttgctt ttctccgggt agtccggcgt
    18181 ccagtcgagg gtggagaagg gccacagtgc cgaggagaac caggtgtcga gcacgtcctc
    18241 gtcccgacgc agcgccttgc cacccgcctg ttcgcgcgct tcggcttcat cgtgagccac
    18301 atagacgcgg ccttcgtcgt cgtaccaggc ggggatgcga tgcccccacc agagctgacg
    18361 cgaaatgcac cagtcctgga tgttggtcag ccactgcgtg taggtagtga gccagttctc
    18421 gggaacgaag cgcacttcgg gatcgccttc ctcggcgtgc gtgcattcca ggcccagctt
    18481 cgccagcatg tccattttca tgaaccactg atcggtcagc atgggttcga tcacggcgcc
    18541 ggtgcggtcg ccgcgcggca ccatcagctt gtgcggctgg atcgattcca gcagaccgtc
    18601 gcgctccagg tcggcgacga tgcgcttgcg tgcttcgaaa cgatcgaggc cgcgataggc
    18661 ttccggcgcg ttgtcgttga tgcgcgcgtc gagcgtcatc acgttgatcg cttcgaggcc
    18721 gtgacgctga ccgacctgcc agtcgttgaa gtcgtgcgcc ggcgtgatct tcacgcagcc
    18781 ggtgccgaac tcgcgatcga catactcgtc ggcgatgacg ggaatggtgc ggccggtgag
    18841 cggcagttgc acggctttgc cgaccaggtc cttatagcgc tcgtcttccg gatgcacggc
    18901 gacggccacg tcgccgagca gggtttcagg gcgtgtggtc gcgacgacca gatgaccctt
    18961 gccgtccacg cgcggatagc ggatgtgcca caggctgccg ctttcctctt ccgagacgac
    19021 ttccagatcg gacacggcgg tgccgagctt gggatcccag ttcaccagcc gtttgccgcg
    19081 atagatgagg ccctcgcggt acatgcgcac gaacacttcg gtgacggttt gggacaagcc
    19141 ttcgtccatg gtgaagcgct cgcgcgacca gtcgcaggac gcgcccatgc gtcgcatctg
    19201 ccgcgtgatg gtcgaacccg actgctcttt ccactcccat acgcgctcga tgaatttctc
    19261 gcggccgagg tcgtggcgcg acagtttctg cgcttcgagc tggcgctcga ccacgatttg
    19321 cgtggcgatg ccggcgtggt cggtgcccgg ctgccacagc gtgttccagc ccatcatgcg
    19381 atggaaacgg gtgagcgcgt ccatgagcgt gtgctggaac gcgtgaccca tgtgcagggt
    19441 gccggtgacg ttcggcggcg gcagcaggat tgcgtaggcg gggcgtccgg gttcgaagcc
    19501 ggccttgaag tagccctggc tttcccagtg cggataccag tgttgttcga tggcgtgcgg
    19561 atcgaagctc tttgcgagtt ccatgaatca ggcaaggaaa ataggcggcg ggatggaccg
    19621 cggattatat cgtgcggcgg gttgtgaaac ccgaaccaca gagacacaga ggcacggaga
    19681 aggatgaaaa tccttaacca ccaagacacc aagacaccaa gaacggcaaa atcgaaacac
    19741 agagatacag agaaccggag ccgccaagat tttctgtcga gccgggaagg ctcaaaatac
    19801 acctcgagtc acgcatgagg cctaccctgc atgccgatta cgcggcagcg tgggaagact
    19861 gacgcatggt tttttggtgt cttcgtgcct tggtggttca gcgtttcgcg tcactccgcg
    19921 ttgaggcgct tgatttcgtc gtcgatgacc tgcgtcacgg cttcccgcac tgcacggcgg
    19981 acttcgactt ccaggtccac gcgtgcgcgc gtcatgactt cggcgacggt cgattcgatc
    20041 cgcggatgca gacgtgcttc gaccagccgc tccagttctg cgcgcaccag ttgcaggatt
    20101 tcggtgcgca gttcgcgctc gaaattcgcc agttcgcttt cgctcatcgg cacgccgcct
    20161 tcgcctggtc gcggcggcgc ctcttcgacg acttcggtca gggtcggaaa atccaccggc
    20221 ggcgcgggct gcctttcggt acgcggcatg cggtggcggg caagcagcgc ctcggccttg
    20281 cgcaatacgt cttccggact gtcgctcatg cgcgttgctc ctcgcggctc aggcggtggc
    20341 gcgttcgccg agattgtggg cgtggatcgg ataaccccga tccttgtaga aacggaagcg
    20401 tgcgcgcgcg tgctgccggt ccgcatcctc cctgccgacg atttcgatca gccggtgaaa
    20461 gcgcgcgaag gaaggcggcc attcgtcgtg cagattcacc agcacgtcct cgtgcgtcga
    20521 cgcgtggtcg tcgtcggcgc tgatcagcac gggcgtttcg gcggccagtc tatggccggc
    20581 gcggcaatgg ggcacgaatg cgatgggcgg cgtggtccac agcatgcggt cgacggtatc
    20641 ggcgacggcg gcgtccgggc tgtagacgac gacgcgtagt ttctgctgca ccgccttggc
    20701 taccagccgg caggcgatgg ccagccggtc ctccgcgtcg tgatagaagt cgatgcgggt
    20761 cacgactgcc cggcgcgatg cagcagataa gtggtgagca agggtaccgg ccgtccggtc
    20821 gcgcccttgt tcttgccgga ggtccacgcc gtgccggcga tgtccaggtg cgcccacgga
    20881 tatttctcgg cgtagcgcgc aagaaagcag gctgccgtaa tgctgccgcc cggccgtccg
    20941 ccgatgttcg ccatgtcggc gaagggactc ttcaactgct cctggtagtc ctcccatacc
    21001 ggcatgcgcc agccgcggtc ccaggattcg cgtcccgcct ccagcagcga ttcggcgagc
    21061 ccttcgtcat tggcgaatac gcccgtggcc acgtggccca gtgcgatgac gcacgcgccg
    21121 gtcagggtgg cgatatccac caccgcggcg ggattgaagc gctcggcata ggtgagggcg
    21181 tcgcacagga tcaaccggcc ttcggcgtcg gtgttgagga tttcgatggt ttggcccgac
    21241 aggctcttga cgatgtcgcc cggcttggtc gcaccgccgc ccggcatgtt ttccgtggcg
    21301 ggaatgaggc cgacgaggtt cagcttgggt ttcagttcgg cgatggcgcg gaaggtgccg
    21361 agcacgctgg ccgcaccgca catgtcgtac ttcatctcgt ccatttcggc cgagggcttg
    21421 atggaaatgc cgccggtgtc gaaggtcacg cccttgccga ccagcacgac gggcttgtcc
    21481 tttttggcac cgccgttgta ctgcagcacc agaaacttcg gcggctcgtg cgagcccttg
    21541 gcgacggcga gcagggcgcc catgccgagc ttttccatgt ccttgcgctc gagcacttcc
    21601 acgccgagct tgtaggtctt cgccaactgc tgcgcctgcg cagccagatg gctgggcgtg
    21661 gcgagattgc tcggccggtt gccgaggttc ttcgcgagtt ccatgccggc ggcgatggcg
    21721 cgcccctcgg ccaccccttc gttgatggcg ttgaggacgg atttttcgct cagcgccacg
    21781 acgaatttct taagcgtcgc ggcgggcgat ttcttgctct tgaattcttc gaagcggtag
    21841 aagacttcgg cggtgaccat gacggcctgg cgcgcggtcc agcgcaggtc gcagccgcgc
    21901 acgggcagct cgaccaggta caaatgcgca tctgcggcgc cggtatcctt gatggcgttg
    21961 gcagccgcgc gcaccgcgtc gcgataagcc ttggcgccga attcggcctc cttgcccagg
    22021 cccaccagga tgagccggtc gcagggaaga ttggccggtt tgagcagcac gcgcgtcgaa
    22081 ccggccttgc cggtcacgtc cttgcgcttg accagttcgg tgatcaagcc tttggcggct
    22141 ttgtccacgg ccgcggcgct gtccgacagc ttgccggatt catacatgcc gacgaccagt
    22201 gcgccggtgg cgagcttgtt cggctctgcg actttcgtgc taaattccaa ggccatctcc
    22261 gttcgtgctg cggtttcgat agggaagcgg gtcgaatggc gaaagattat ccccgctttg
    22321 tttcagcccc gtcaaaacgc gcgcggtatg cgtcgggcgc gggcacccga caggacagac
    22381 gacgcgccag ggccggcatt ccccagtgat ttttcaacgc tcgctattgc gcgaattcgg
    22441 caactacgcc gctgcggtat tcgccgcact gttcgccatc atcgtcacga gccagctggt
    22501 gcgcatgttg gggcgcgccg caggcggcag catcccggcc gatgcggtgt tcgcgctcat
    22561 cggcttcacc gccatcaact atctccccgt gctgctcgcg ctgacgctgt tcgtcgcggt
    22621 gctgatgacc gtctcccgcg cctaccggga ttccgagatg gtgatctggt cggcttccgg
    22681 cgtctcgctg ctcgcatgga tccgtccggt actgatgttc gccgcgccac tggtggcggt
    22741 catcgcgctc atggcgctgc tgctgtctcc atgggccaac aatcgcgcgg acgaataccg
    22801 ccggcagatc gaaagccgcg acgaggtctc gcgcatcgcg cccggcgtgt tccgagagtc
    22861 ggccaacgcc gaacgcgtgt tcttcgtcga aagcgccgat gtggacgacg gacgggtgaa
    22921 gaacgtgttc atcgccagca agcagcacgg caagaccggc gtggtggtga gcaaggaagg
    22981 ccatatcgaa gtgcacgaca acggcgaccg cttcgccgta ttgctcaacg gccggcgcta
    23041 cgagggcctg cccggcacgc cggagtaccg catcacagag ttcgagcgct atgccgcgcg
    23101 catccaatcc ggcgagcagc aggccggtcg cggttcgccc aagacacttt ccactgctga
    23161 actgatcagg gaccccacac cgcgcgggca ggcggaactg gtctggcgca tcggtctgcc
    23221 cttcgtcgcc gtcaatctgg cgctgctggc cattccgctc ggtttcgtca atccgcgcgc
    23281 cagccggtcc gtgaacctgc tgttcgccgt cttcgcctac atgctctaca acaacctgat
    23341 cagcgtggtg cagtcctggg tgtcgcagga gaaactgagc ttcggcatag gctggtgggt
    23401 gctccatgcg gtggcgctgg gcgtcgtcgc cctgctgttc gcctggcgca tgagcctggg
    23461 ttttgctttc ctgcggcggc cgcgatgacg atcatcacgc gctatctcag ccgggaaatc
    23521 tacaaggcga cggctttcgt gctgttcgcc tttctggcgt tgttcgcctt cttcgacctg
    23581 atcaacgaac tcgaccattt aggcaagggc acctacggtc tgccgcaggc ctttctgttc
    23641 gtgatgctct cgttgcccgg ccatatctat gagctctttc cgatcgtcgt gctgatcggc
    23701 accctggtgg cgctgtcgaa tctggcccac cattcggaat acaccgtcct gcgcgtctcc
    23761 ggcttctcgc cctggcgcgc cggcgcgaca ctcacgcgca tcggcatggc tttcgtgatt
    23821 gccgccattc tggtgggcga gcttgccgcg ccggccgcgg agcagacagc acaaaggctg
    23881 cgcctggaca gcctggggcg cacggtcgcc caggaattgc gcacgggctt gtgggtgagg
    23941 gccgactcca aattcgtcaa tatccaggag gtgtcgcccg actcgcgcct caaaggcgta
    24001 cgcatttacg agttcgatga gcagatgcac ctgcgctcga tcagcgaagc ggccgcaggc
    24061 gagtatctga acggcagcac atggcgtctc agtgacgtgc agcagacaca gttcacggcg
    24121 gacggtgcgc acgtggaaaa gctgggcacg atcgaatgga agaccgtgat cacgcccgac
    24181 atgctggcgg tgttgctggt acgaccggag aaaatgtccg cctgggcgct ttatcaatac
    24241 acgcggcacc tggcgaagaa caaccagcgc accgagcgct acgaaatcgc gctgtggaag
    24301 aaggtcgtct atcccttcgc ggcgctggtg atgatgggcc tggcactacc cttcgcctat
    24361 gtcctggtgc gggcgggcgg cgtgggcgcc aagctctttg ccggcattat gctgggcgtg
    24421 ttgttccact tcctcaacac gctgttttcc catctaggcg tgctgcaggg ctggccaccc
    24481 tttctcagtg ccacgctgcc gagcgcgatc ttcctgctga tcgccgtggg catgatgcgg
    24541 tgggtcgagc ggcgctgagc cgccaaaagg cagtcgagag tcgagagcgg agagtcggcc
    24601 gttcatgatt cgactcgacc ctggcgggcc ggctcaccgc gaacggctac gcttcaagct
    24661 ttcgtttttt cggccgctgc gccggattcc tcgcagcggc cgggaatgac ggtacttcta
    24721 aggcttcgac ttgcgcggct ccttgaccag caccagtcgc gtgccggcca gccggtcgtg
    24781 gaggaactgg tcgtcccggt cgaggagggc ccaggcgagg ctgaacaggc cgacgcccac
    24841 cgcaagccac gcgccgatca ggtcgtgttg cttccacaat gcgattgcgc cgaccacgcc
    24901 gccgcccagg gtcagcgctg ccagcacgaa acgcagcaga gcttgccaca tcgacagtgg
    24961 gccgccgtcg ggccggacga cgcgtagttt ccacgcccgc atcgccaggg tgcggccctt
    25021 gtgccagcac cagacgaaat aggcgcccag aatcagcagc agataaagtt ggaacgtgag
    25081 ccgcacgccg ccgtgcagtt cgccggtggc ggcgccgacg aaaaggaaag cggcccccag
    25141 caggatggcc gtgacgatca gcgcttcgta gaccatggcc gccaggcgtc ttgcaatgga
    25201 cggatagatg cccgccaggc gctggtcgga tggggaagga gaggagaaat cctgcttcaa
    25261 gctacgtccc ggccgcctgt cgtcggcggc gggtttccgg ttgcgggaac ggggcggcat
    25321 cttagcaggc tctgatcgag gttgtcggcc ggcgcagggc cagctcgtct gtctaagggc
    25381 ttgattgcgt tgttctcgcc tgccgggggg cgacgcgatt gcgatcgtcc acgacccttg
    25441 ttccgacttc cccgaagcgg cgtactgtca ggagatcggc aacttgacca atcggggcgc
    25501 aactattact gttagcggtt ttcgccgaat attcaacgca cttaggtcgc ccgcatgccc
    25561 accgccaaag aaccccgtgc tcccgccctg accggggcgg atatcgtcat caaatgcctg
    25621 gaggcggagg gggtcgaata cgtattcggc tatccgggcg gcgcggttct ttatatctac
    25681 gatgcgttgt tcaagcagga caaggtcaag cacgtcctgg tgcgccatga acaggcggcc
    25741 acgcatgcgg cggacggtta ttcccgcgca agcaacaagg tcggcgtcgc gctggtgacc
    25801 tccggtcccg gtgtaacgaa cgccgtcacc ggcatcgcaa cggcttatct cgattccatt
    25861 ccaatggtgg tcatcagcgg ccaggtgccg acgcacgcca tcggtctgga cgcgttccag
    25921 gaggtcgata ccgtcggcat cacgcggccg tgcgtgaagc acaacttcct ggtgaagcgc
    25981 gtggaagaca tcgcgcccac cctcaagaag gcattccatg tggcgaccac cggtcgtccc
    26041 ggtccggtgc tggtggacat tccgaaggat gtcaccacca tggtgcatcc cttcaactat
    26101 cccgaaaagg tggagatgcg ctcctacaac ccggtgctca aaggccatcc gggtcagatc
    26161 aagaaggcgg cgcaactcat cctcgaagcc aagcgccctt atgtttacgc gggtggcggc
    26221 gtgattctcg cccatgccgc ggacgaactg accaagctgg tcaagaagct cggctatccc
    26281 tgcaccaaca cgctgatggg tctgggcgcg tttcccgcga ccgaccgcca gttcgtcggc
    26341 atgctcggca tgcacggcac ctacgaagcc aacatggcca tgctgcattg cgacgtgctg
    26401 atcgcgatcg gcgcgcgctt cgacgatcgc gtgatcggca atacggccca cttcggccag
    26461 gtgccgcgca agatcatcca tatcgacatc gatccgtcgt cgatttcgaa gcacgtgaag
    26521 gtggacgtgc cgatcgtcgg cgacgtgaag gagacgctcg aggaactgct gtcacacatc
    26581 gacggcaaga ccagcaagat cgatcagaag gcgctgcagg cctggtgggc gcagatcgaa
    26641 ctgtggcgtg gcaaggattg cctgcgctac gaccgcacga gcgacatcat caagccgcag
    26701 ttcgtcatcg agaagttcta tgagttgacc ggcggagacg cctttgtctg ctccgacgtc
    26761 ggtcagcacc agatgtgggc ggcccagttc tacaagttcg acaagccgcg ccgctggatc
    26821 aattccggcg gtctgggcac catgggtttc ggtctgccgg cggcaatggg cgctttcctc
    26881 gccaacccgg atgcgaccgt tgcctgcgtg accggcgagt cgagcatcca gatgtgcatt
    26941 caggaactgt cgacctgcag gcagtaccac ctgccgatca agatcctgaa cctcaacaac
    27001 cgctatatgg gcatggtgcg gcagtggcag cagttcttcc acggcaaccg ctacgccgag
    27061 tcctacatgg acgcactgcc ggatttcgtg aaactcaccg aaagctacgg ccatgtcggc
    27121 atgcggatcg agaagccggg cgatgtggaa ggcgcgttgc gcgaagcgct caagctgaag
    27181 gaccgcctgg tgttcatgga cttcatgacc gatcagaccg aaaacgtcta tccgatggtc
    27241 gccgccggca agggcatttc ggaaatgatt ctggcggagg aactgtgaaa tgaatgcacc
    27301 cgccagcccc tatctcctca gctccgatcc gtctcgcgcc atgcgccaca tcatttccgt
    27361 cctgctggag aacgaatccg gcgcgctgtc ccgcgttgcc ggtctgttct ccgcgcgcgg
    27421 ctacaacatc gaatcgctga ccgtcgcgcc gaccgaggac gcctcgctgt cgcgcatcac
    27481 gctcgtcacc accggttccg aagaggtggt cgagcagatc accaagcaac tcaacaagct
    27541 gatcgaggtg gtgaaggtcg tcgatctgtc cgaggcgccg cacatcgagc gcgaactcat
    27601 gctgatcaag gtgcgcgcca ccggccgcga tcgcgaagag atgaaacgca tggccgacat
    27661 cttccgcggc cgcatcatcg acgtgacgga cagcacctac accatcgagc tcaccggtac
    27721 cggcgacaag ctcgacgctt tcatccatgc gctggacaag accgccattc tggagaccgt
    27781 gcgcaccggc gcatcgggca tcgggcgcgg cgagcgcgtg ctacgggttt aactacagtc
    27841 gagagtggag agccgagagt cgagcgtggc gcttctcgcc gcctttgctc tccactctcc
    27901 gctcccggct ctccactctt tagaacttaa ggatatcgac catgaaggtc ttttacgaca
    27961 aggacgccga cctctcgctg atcaagggca agaaggtcac gatcgttggt tatggctccc
    28021 agggccatgc gcatgcattg aacctcaagg attcgggcgt ggacgtgacg gtcggcctgc
    28081 gcaagaacgg cgcttcctgg ggcaaggcga ccaatgccgg tctgaacgtg gccgagatcg
    28141 ccgaggccgt gaagggcgcc gacgtcgtga tggtgctgct gcccgatgag cagatcgcca
    28201 acgtctataa gcgggaaatc gagccgaaca tcaagcagaa cgcttcgctc gccttcgccc
    28261 acggcttcaa catccattac ggccaggttc agccgcgtgc cgacctcgac gtgtggatgg
    28321 tcgcacccaa ggcgccgggc cataccgtgc gcagcaccta tacccagggc ggcggtgtgc
    28381 cgcaactcat cgcggtctat gcggacaaca ccggcaaggc gcgcgatctg gccctgagct
    28441 atgccgcggc aaacggcggc ggccgtgcgg gcattatcga aacgaccttc aaggaagaga
    28501 ccgagaccga cctgttcggc gagcaggccg tgttgtgcgg cggcgccgtc gagctgatca
    28561 aggccggctt cgagacgctg gtcgaggcgg gctacgcgcc ggagatggcg tatttcgagt
    28621 gcctgcacga attgaaactg atcgtcgacc tgatctacga gggcggcatc ggcaacatga
    28681 actactcgat ctcgaacaac gccgaatacg gcgaatacgt caccggcccg aagatcgtga
    28741 ccgaagagac ccggcaggcc atgcgccagg cgctcaagga catccagacc ggcgagtacg
    28801 ccaagagctt cattctggag aaccacgccg gtgcgccgac gctgctgtcg cgtcgtcgcc
    28861 tgacggccga gcaccccatc gagcaagtgg gcgagcagtt gcgcgccatg atgccgtgga
    28921 ttaaaaagaa caagctggtc gatcaatcga aaaactgaca aaacgcgggc cggaattccg
    28981 gcccgcgcgc tttgagtccc acaacccgca tggctagccc ttatcctcat cccatcctcg
    29041 ctcgcgaagg ctggctctat tcctttctcg ccgttgcggc ggccgtcggg gtttgcgtgt
    29101 tcgccggctt ctggtggtcg gtgccgttct ggatcgtcgc gatcttcgtg ctgcagtttt
    29161 tccgcgatcc ggcgcgcgag gtgcctggcg attcgcgcac cgtcgtctgc cctgccgacg
    29221 ggcgcgtgct gatggtcgtg ccggcggaag atccttatct caaccggcct gcaatcaaag
    29281 tcagcatctt catgaacgtg ttcaacgttc attccaaccg ctcgccggtg gacggggagg
    29341 tggttgacgc ctggtacacg cccggttcgt tcttcaacgc cgatctcgac aaggcttcgc
    29401 tggagaacga gcgaaacgcc ttgcacctgc gcaccgactc cggcgtggac gtcacctgtg
    29461 tgcagattgc cggactcatt gcgcggcgcg tgctttgcta cgtcaagccg ggtgaccggc
    29521 tcgtgcgcgg gcagcgtttc ggctttattc ggttcggttc gcgtgtggac gtttacctgc
    29581 cgccgggtac gcgtgtgaag gtggaactgg gtcagaaggt gcacgcatcg agcaccgtct
    29641 tagcggagtt ggaggcatga gcgaatatca aactcaaacg cgcgacaatc tgcgagggac
    29701 gctgcgtcat cgcggcatct atctgttgcc caacctgttt acctctggcg tgctgttctc
    29761 gggcttctac gccatcgtgc aggcgatgaa cggacgctat gaactggccg cgacagcggt
    29821 gttcgttgcc atggtgctcg acggcctcga cggccgtatc gcccgtctca cgcgcaccca
    29881 aagcgaattc ggcgcgcagt acgacagtct ggccgacgtc gtcgccttcg gcgctgcgcc
    29941 ggcgctgatc atgtacgtgt gggctttgca gccgctcggc cggatcggct ggatcgtttc
    30001 cttcgtctat tgcgtatgcg tcgcgctgcg tttggcgcgc ttcaatacca atatcggcgt
    30061 agtcgacaag aaatggttcc aggggctgcc cagtccgtcg ggcgcggcgc tggtcgccgg
    30121 cctcgtctgg atcatgaacg acctgggttt cgatccggtc ggcttcgggc gcgcgctcgc
    30181 ggtgatcttc accctgttcg ccggcatcac catggtgagc agcgtaccgt tctacagctt
    30241 caagaccatc aatttccgtc gcgccgtgcc gttctgggcg attcttctgc tcattctggt
    30301 actggtgatc gtcacttcgc acccgccgct ggtgctgttt atcttcttcg tggcgtattc
    30361 gctgtccggc tacatcatgt ggctctggaa gttccgccac aagcgttcgc ctttctgagg
    30421 agggcatcat gagcgccgat cgtctgatta ttttcgacac taccttacgc gacggagagc
    30481 agagccccgg cgcttccatg acgcgcgaag agaaagtgcg catcgccaag caactggagc
    30541 ggctgcgggt cgacgtgatc gaggcggggt tccccgccgc atccaacggc gacttcgagg
    30601 cggtgcgggc ggtcgccaat gcgatcaagg acagtcgtgt ggccgggttg tgccgcgcca
    30661 acgaacgcga tatcgatcgc ggcgccgaag cgctcaagga ggcggcgagc gcgcgtatcc
    30721 acaccttcat cgcgaccagt gcgctgcaca tggaaaagaa actgcgcatg tcgcccgagc
    30781 aggtgctcga gcaggcgcag gttgcggtga agtacgcacg caagtattgc gacgacgtcg
    30841 agttttcgcc cgaggatgcg agccgctccg atccggagtt tctgtgccgc gtgatcgagg
    30901 cggtgatcaa ggccggcgca cgcaccatca atattgccga caccgtcggt tatgccgtgc
    30961 cgcaacagta cggcgaattc atccgcagcc tgcgcgaacg cattccgaac tccgaccagg
    31021 ccatctggtc ggtgcactgc cataacgacc tgggcatggc ggtcgccaac tcgctggccg
    31081 gcgtgcacat cggcggcgcg cgccaggtgg agtgcaccat caatggcctg ggc
//
LOCUS       DATGOU010000006         5455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3129873, whole genome shotgun sequence.
ACCESSION   DATGOU010000006 DATGOU010000000
VERSION     DATGOU010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3129873"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VKZ48_00385"
     CDS             complement(<1..650)
                     /locus_tag="VKZ48_00385"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HLU76339.1"
                     /translation="MQEIIQRLLEGIGIVSAESWAAFLYSAVRVLLIVIAALIVLAVL
                     HRLINAFKNRLIQKDPDPEQLKRVETLARVYGYIASVTVYTLTGMLILAELGISIAPV
                     LATAGVAGVAVGFGAQSLIKDYFTGFCLLVENQIRQGDVVNIAGNGGYVEEVTLRYVR
                     MRDFDGNVHFVPNGNISTVINMTRDFAFSVIDVRVGYREDIDRVIEIIKDVAREQR"
     gene            complement(859..1701)
                     /locus_tag="VKZ48_00390"
     CDS             complement(859..1701)
                     /locus_tag="VKZ48_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HLU76340.1"
                     /translation="MRNTRLLRIGLSPRFMHNVPLEMGVGRRGVQYLVESIAHWVMSR
                     DALIFMIPSIESGGLIRRGNLSVADYVSELDGLVLQGGADVSPVTYGEVPMQESWQGD
                     RVRDLYELELLRAFVAQKKPVLGICRGLQLINVAFGGTLYQDIATQLPNTLGHHDAEA
                     YDQNFHEIEFVSGGGLAGLYPDREAARVNTIHHQAVKDLGENLKVEAHAKPDRVIEAI
                     RWEGDSYVFGVQWHPELHDHRDPSQLDGTPILNEFLQCARERAGMLTAESQEQAQALA
                     AAAV"
     gene            1864..4308
                     /locus_tag="VKZ48_00395"
     CDS             1864..4308
                     /locus_tag="VKZ48_00395"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLU76341.1"
                     /translation="MDTFFETGLDLFSSDEPVGVAERLHRPSSPAVEQLVAALERRHA
                     GRIVGRTVRPAQPARYADFPEGVNPRLRAALAARGISRLYSHQRECWDLATNGQDVVV
                     VTPTASGKTLCYNLPVLHTALASKTKALYLFPTKALAQDQVNEILELNRAADLGVRAY
                     TFDGDTPADARKAVRVHGDIVVSNPDMLHQSILPHHTKWAQFFENLRYVVIDELHTYR
                     GVFGAHVANLLRRLLRICRYYGTRPTFILCSATIANPQELAERLLGGPVTLIDRSGAP
                     QGERHLLLWNPPVLNPDLGLRASARSQSTRIARLALRMKLKTIVFANSRLEVEVLTKY
                     LKDVYDRDPRKPPRITAYRGGYLPTERRDKERKLRAGQLDAVVSTSALELGMDIGSLD
                     VCVLNGYPGSIAAALQRFGRAGRRQRAALGVLVCNSDPLDQYLALHPEFFFDASPEHA
                     RVDPDQLLILMDHIRCAAFELPFRRGEQFGKEQLEVLLSYFEDQAVLHREGDTWHWIA
                     DGYPANTVSLRSVAEGNFAVIDITDGGKNVIAEVDYSSAALTLYEGAIYMVQASPWQV
                     ERLDWVGRKAFVRKTDADYYTDAIDYTKLKLLDEFEACERDGAKCAHGEAHVLRRVAG
                     YKKIRYYTHENIGYGNVHLPDYDMHTTAVWWQLREQVLERAFASRYEALDGFLGAAYA
                     MHHIAALLSLAEPRDLGRSVGDGAAQWSAVVDADGRSSARSLSGEPIDLAQRAFRPTV
                     YLYDNFPGGIGLSAPLYDLRDQVVTRAIELIEQCRCLQGCPACVGPILGEHGHAVQTP
                     KAAALLVLWLFKGSGR"
     gene            4305..5204
                     /locus_tag="VKZ48_00400"
     CDS             4305..5204
                     /locus_tag="VKZ48_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HLU76342.1"
                     /translation="MSAQLAARLRRLRPAHQMLPANDAHDAALAELMARVARLRGRAR
                     EIARMRPRLDIAALGGRWIDEHLLCVERRYTPACRHGDMSLRRLLQLRHDRSGLLGVR
                     QALDPRRIVFLDTETNGLSGGAGTLAFMIGMARLEGELLVTRQYVITRYGAEGAMLDR
                     LARDLADAVHLVSYNGKRFDVPLLQARLQLHGRDDAFAALEHIDLLHALRRARRHRPD
                     LAFMSDCRLQTAEARLLRLERRDDLPGQEVPRAWEQLLRDGDANLMRRALEHNRLDVL
                     SLGALLALFAESAGHLLGCAAAD"
     gene            5258..>5455
                     /locus_tag="VKZ48_00405"
     CDS             5258..>5455
                     /locus_tag="VKZ48_00405"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HLU76343.1"
                     /translation="MLPNLKILGIVGSLRRESHNLKLMRVAQRRMPGGAQLDIFTLEG
                     IPPFNQDEEQQPPPKVIELKNA"
BASE COUNT          948 a         1831 c         1723 g          953 t
ORIGIN      
        1 ttgcgctgct cacgcgccac atccttgatg atttcgatga cgcggtcgat gtcctcgcga
       61 tagccgacgc gaacgtcgat caccgagaag gcgaagtccc gcgtcatgtt gatcacggta
      121 ctgatgttgc cgttaggcac gaaatgcacg ttgccgtcga agtcgcgcat gcgcacatag
      181 cgcagggtca cttcctccac atagccgccg ttgccggcga tgttcaccac gtcgccctgg
      241 cggatctggt tctccaccaa caggcaaaat ccggtgaagt aatccttgat caggctttgc
      301 gcgccgaagc cgacggccac gccggccacg ccggccgtcg ccagcaccgg cgcaatcgat
      361 ataccgagct ccgcaaggat cagcatgccg gtgagggtgt acaccgtcac cgatgcgata
      421 tagccgtaca cgcgcgcgag cgtttccacc cgcttgagtt gctcgggatc cgggtccttc
      481 tggatcaacc ggttcttgaa tgcattgatg aggcgatgca ggaccgccag cacgatcagc
      541 gcagcgatga cgatcagcag gactcggacg gcggaataga gaaaggcggc ccaggattcg
      601 gcgctgacga tgccgatccc ttcaagaagg cgttggatga tctcttgcat gactcgcttc
      661 gtcgatgagg tggaaagaca cgccggcaca gcggcgacga tggcactccc tcatacggcg
      721 ccgctggaag cggccggccg atgcgcgatc gcgcgcaccg gatcaggagc ttcgcgcgag
      781 caatgctgcg cgaaaggcgg gcattgtgtc acagcgcgcg cgggtgcgtc gacccgccgg
      841 gcgcgctgtc agagaggatc agactgcggc ggcggccagc gcctgcgcct gttcctgcga
      901 ttcggcggtg agcatgccgg cacgctcgcg cgcgcactgc aggaattcgt tgagaatcgg
      961 cgtgccgtcg agctggctcg gatcgcggtg atcgtgcagc tccggatgcc attgcacacc
     1021 gaacacgtag ctgtcgccct cccagcggat cgcctcgatg acgcgatccg gcttggcgtg
     1081 cgcctccacc ttcaggtttt cgcccaaatc cttcacggcc tgatggtgaa tggtattgac
     1141 ccgtgcagct tcgcgatccg gatagagacc agccaggccg ccgcccgaaa cgaattcgat
     1201 ttcgtggaaa ttttgatcgt aggcctcggc atcatgatgg cccagcgtgt tcggtaattg
     1261 cgtggcaatg tcctgataca gcgtgccgcc gaacgcgacg ttgatcagtt gcaggccacg
     1321 gcagatgccg agcaccggct tcttctgcgc cacgaaggcg cgcagcaatt ccagttcgta
     1381 gagatcgcgc acgcgatcgc cctgccagct ctcctgcatg ggcacttcgc cgtaggtcac
     1441 ggggctgacg tcggctcccc cctgaagcac caggccgtcc agttccgaga cgtaatcggc
     1501 cacgctgaga ttcccgcggc ggatcaggcc gcccgactcg atggaaggga tcatgaaaat
     1561 cagcgcatcg cgcgacatca cccagtgcgc gatcgactcg accagatatt gcacgccgcg
     1621 tcggcccacg cccatctcga gcgggacgtt atgcatgaag cggggcgaca ggccgatccg
     1681 cagaagccgg gtgttacgca tgacgcctct cattcttcca cccttgtccg cgcttttcga
     1741 gtcctcactg tgactttggt tgcgcgaacg ctacaaagat gggcgcttcg ggcctgaggc
     1801 tcatcgggcg agcctgtgaa ccggcgatgc tcgaaccgta ttgtgggatg agtaggctgc
     1861 tgcatggaca cattctttga gaccgggctc gatctgttca gttcagacga gcccgtcggc
     1921 gtcgccgaac gcctgcaccg accgtcgtcg cccgcggtgg agcaactggt cgccgcgctc
     1981 gagcgccggc acgccggacg catcgtcggc cgcacggtca ggccggcgca gcccgcgcgc
     2041 tacgccgatt tcccggaagg cgtgaatccg cgcctgcgcg ccgcgctcgc cgcgcggggg
     2101 atcagccgcc tttacagcca tcagcgcgag tgctgggatc tggcgacaaa cgggcaggat
     2161 gtcgtggtcg tcacgcccac ggcctccggc aaaacgcttt gctacaacct gcctgtcctc
     2221 cataccgcgc tggctagcaa gaccaaggcc ttgtacctct tccccaccaa ggcgctcgcg
     2281 caggatcaag tcaacgaaat cctcgaattg aatcgcgccg ccgatctggg cgtgcgcgcc
     2341 tacaccttcg acggcgacac acccgccgac gcacgcaagg cagtccgcgt gcatggcgat
     2401 atcgtcgtct ccaatccgga catgctgcac cagtcgatcc tgccgcatca caccaagtgg
     2461 gcgcagttct tcgaaaacct gcgttatgtg gtcatcgacg aactgcatac ctatcgcggc
     2521 gtgttcggcg cgcacgttgc caacctcctc cgccggctgc tgcgcatctg ccgctactac
     2581 ggcacgcgcc cgacgttcat tctgtgttcg gcgacgatcg ccaatccgca ggaactggcc
     2641 gaacggctgc tcggcggccc cgtcaccctg atcgaccgct ccggtgcgcc gcagggcgag
     2701 cggcatctgc tgttgtggaa cccgcccgtg ctgaatcccg atctgggatt gcgcgcgtcg
     2761 gcgcgttcgc agtcgacgcg cattgcgcgg ctggcactgc ggatgaaact gaagacgatc
     2821 gtgttcgcca actcgcgcct ggaggtggag gtgctgacca agtacctcaa ggacgtttac
     2881 gaccgcgatc cgcgcaagcc gccacgcatc accgcctatc gcggcggcta tctgcccacc
     2941 gagcgccggg acaaggaaag gaagctgcgt gccgggcaac tcgatgcggt ggtctccacg
     3001 tccgcgctcg aactcggcat ggatatcggc agcctggatg tgtgcgtgtt gaacggctat
     3061 ccgggcagca tcgccgcggc gctgcaacgc ttcggccgcg ccgggcgccg ccagcgcgcc
     3121 gcgctcggcg tactggtttg caacagcgat ccgctcgatc agtacctcgc gcttcatccg
     3181 gaattcttct tcgacgcctc gccggagcac gcgcgcgtcg acccagatca gttgctcatc
     3241 ctcatggacc atatccgctg cgcggccttc gaactgccgt tccgccgcgg cgagcagttc
     3301 ggcaaagagc aactcgaagt actgctgagc tacttcgaag atcaggccgt gctgcatcgc
     3361 gaaggcgaca cctggcactg gatcgccgac ggttatcccg ccaacaccgt cagcctgcgt
     3421 tccgtcgccg aaggcaactt cgccgtgatc gacatcaccg acggcggcaa gaacgtgatc
     3481 gccgaagtcg attattccag cgcggcgctg acgctgtacg aaggcgcgat ctacatggtg
     3541 caggcctcgc cctggcaggt ggagcggctc gactgggtgg gccgcaaggc tttcgtgcgc
     3601 aaaaccgacg ccgactacta caccgacgcc atcgactaca ccaagctcaa gctgctggac
     3661 gagttcgaag cctgcgagcg cgacggcgca aaatgcgcgc acggcgaagc gcatgtgctg
     3721 cggcgtgtcg ccggctacaa gaagatccgc tactacacgc acgagaacat cggctacggc
     3781 aatgtgcacc tgcccgatta cgacatgcat accaccgcgg tgtggtggca gttgcgggag
     3841 caggtgctgg aacgtgcgtt cgcttcgcgt tacgaagcac tcgacggttt tctcggcgcg
     3901 gcctacgcca tgcatcacat cgccgcgctg ctgtcgctcg ccgaaccgcg cgatctgggc
     3961 cgttcggtcg gcgacggcgc cgcgcagtgg tccgccgtcg tcgatgccga cggccgcagc
     4021 agcgcgcgca gtctttccgg cgaaccgatc gatcttgcgc agcgcgcttt ccgtcccacc
     4081 gtttatctgt acgacaactt tcccggcggc atcggcttgt ccgcaccact ttacgacttg
     4141 cgcgaccagg tcgtcacccg cgccatcgag ctgatcgagc agtgccgctg cctgcaggga
     4201 tgtccggcct gcgtcggccc catcctcggc gagcacgggc acgccgtgca aacaccgaag
     4261 gccgccgccc tgctggtgct gtggctgttc aaggggtccg gccgatgagc gcgcagctcg
     4321 ccgcgcgcct gcgccggttg cggccggcgc atcagatgct gccggccaat gacgcgcacg
     4381 acgctgcgct ggccgagctc atggcgcggg tggcccggtt gcgcggccgt gcgcgcgaga
     4441 ttgcgcgtat gcggccccgc ctcgacatcg ccgcgctggg cggccggtgg atcgacgagc
     4501 atctgctgtg cgtcgaacgc cgctatacgc cggcctgccg tcatggcgac atgtccctgc
     4561 gccgactgct gcaactgcgc catgaccgca gcggcctgct cggtgtgcgg caggcgctcg
     4621 atccccggcg catcgtgttt ctggataccg agaccaacgg tctgtcgggc ggcgcgggca
     4681 cgctcgcctt catgatcggc atggcgcgtc tggagggcga actgctggtt acgcggcaat
     4741 acgtcatcac gcgctatggc gcggagggcg caatgctcga tcggctggcg cgcgatctgg
     4801 ccgacgcggt gcatctggtc agctacaacg gcaagcgctt cgacgtgccg ctgctgcagg
     4861 cgcgcctgca actgcacggc cgcgacgatg ccttcgccgc gctcgaacac atcgatctcc
     4921 tgcatgcact gcgtcgcgcc aggcggcatc ggcccgatct ggccttcatg tccgactgcc
     4981 ggctgcagac cgccgaggcg cggctgctgc gcctggagcg ccgggacgac ctgcccggcc
     5041 aggaagtgcc gcgcgcgtgg gagcaattgc tgcgcgacgg cgacgcgaat ctgatgcgcc
     5101 gcgcgctgga acacaaccgg ctggacgtgt tgagtctggg cgcgctgctt gcgctgttcg
     5161 ccgagtccgc aggccacctc ctcggctgcg ccgcggcgga ctgagacagg cgcgcggctt
     5221 gcctacacct gcgagaaccg ctcgcaaagg gaagaccgtg ctaccgaatc tcaaaatcct
     5281 cggcattgtg ggcagcctgc gccgcgaatc ccataacctg aaactgatgc gtgtcgccca
     5341 gcgacggatg ccgggcggcg cccaactgga tatcttcacc ctggagggaa tcccgccttt
     5401 caatcaggac gaggagcagc aaccgcctcc caaagttatc gaactcaaga atgcg
//
LOCUS       DATGOU010000007        16378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_911332, whole genome shotgun sequence.
ACCESSION   DATGOU010000007 DATGOU010000000
VERSION     DATGOU010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16378
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911332"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..880
                     /gene="panC"
                     /locus_tag="VKZ48_00410"
     CDS             50..880
                     /gene="panC"
                     /locus_tag="VKZ48_00410"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HLU76344.1"
                     /translation="MKIIRSIPELRAALKGREIACVPTMGNLHEGHLQLMRLAKQEAS
                     TVVATIFVNRLQFAPHEDFDTYPRTFEEDRAKLEREGVDVLFAPDERQMYPEPQGFHV
                     EPPDYANILEGEFRPGFFRGVATVVLKLLNIVQPDVAVFGKKDYQQLMIVRNMVRQLN
                     LSVRIVAGETARAADGLALSSRNGYLSTTERAQAILLYRVLQDTAAAIQAGNRDYAAL
                     ETAAVERLRQENWLPDYVAVRKQADLQSPAAVDRQLVILAAARLGTTRLIDNLEVAAP
                     "
     gene            971..1351
                     /gene="panD"
                     /locus_tag="VKZ48_00415"
     CDS             971..1351
                     /gene="panD"
                     /locus_tag="VKZ48_00415"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HLU76345.1"
                     /translation="MQRTMLKSKIHRATTTHSELHYEGSCAIDEDLLDAADIREYQQI
                     DIFNVTNGERFTTYAIRAQRGSGIISVNGAAARKAAVGDILIIASYAVYNEIELERFE
                     PDLVYVDSRNRIKHQGKQIPLQRA"
     gene            complement(1412..2008)
                     /locus_tag="VKZ48_00420"
     CDS             complement(1412..2008)
                     /locus_tag="VKZ48_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HLU76346.1"
                     /translation="MLEGIRVAILVAEGFEQSELTEPKRALEAAGAQTFIVSPEQHEV
                     QGWRHFDKADRFHVDVQLEQARAEEYDALVLPGGVANPDQLRTQPKAVQFIKTFFDAD
                     KPVGVICHGPWTLIEAGVVKGRTITSWSSLKTDLVNAGAKWVDQEVAVDGALVSSRKP
                     ADLPAFNKRLIEIIAEGPRGGRSAEARPTGPIADAPTG"
     gene            complement(2171..3112)
                     /locus_tag="VKZ48_00425"
     CDS             complement(2171..3112)
                     /locus_tag="VKZ48_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HLU76347.1"
                     /translation="MNFQQLRYVRETVRRGMSLTEAANALYTSQPGVSKQIKELEEEL
                     GVLIFERRGKRFIGLTEPGKAVLEIIERVLDEAENLKRVGSDFADRDSGSLIVAATHT
                     QARYALPKVVGAFRQRYPRVHLSLLQGNPQQVAQMVMRNDAHVGIASESLSLYPELLA
                     MPGYQWHHVVVVRDGHPLLKSGRLTLEKLAEYPIITYSPEFTGRSQINDAFAARELNF
                     DVVLTAIDSDVIKTYVELDLGVGIIAAMAFDPQRDRGLKAIDAGHLFRASTTRIAIRR
                     NAYLRSYTYDFLQLFAPQLTREVIQQALQGGGESYEL"
     gene            3306..4055
                     /locus_tag="VKZ48_00430"
     CDS             3306..4055
                     /locus_tag="VKZ48_00430"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006225124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HLU76348.1"
                     /translation="MTAISDSITIDADPGERSQALVDMLQQIAHDYAPAALASSLSIE
                     DMVVTDAILRHDIPLEIFTLDTGRMHQDTLDLIDTIERHYGTRIRVYLPDPKAVMRYV
                     AQHGRDAFYESVELRRQCCAIRKTEPLKRALAGKRAWITGLRRSQSVTRAALPLQEYD
                     TTHGLEKFNPLAEWSEQEVWAYLYANDVPYNRLYEQGYRSIGCAPCTRPTVTGEDVRA
                     GRWWWEQPETKECGLHVGPDGRLVRSKGKAS"
     gene            4052..4987
                     /gene="cysD"
                     /locus_tag="VKZ48_00435"
     CDS             4052..4987
                     /gene="cysD"
                     /locus_tag="VKZ48_00435"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003821534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HLU76349.1"
                     /translation="MSAVLQERQVAELPTRSHLDWLESEAIHIMRELAGECANPVVFF
                     SGGKDSIVLLRLAEKAFRPGRFPFPMLHIDTGHNYPEVIAFRDRRAEELGERLIVRSV
                     EDSIRRGTVRLRSETESRNAAQAVTLLEAIAEFGFDACIGGARRDEEKARAKERVFSF
                     RDEFGQWDPKNQRPELWSLYNTRVHKGEHMRVFPISNWTELDVWQYIAREKLEVPSIY
                     YAHQRPVVRRNGMLVPVTRLTPAREGERIEELSVRFRTVGDISCTCPVESTARDPAEI
                     IAETAITTITERGATRMDDKTSDASMERRKREGYF"
     gene            5116..6408
                     /locus_tag="VKZ48_00440"
     CDS             5116..6408
                     /locus_tag="VKZ48_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLU76350.1"
                     /translation="MSVLGELERGRLNAEDRGVLRFITAGSVDDGKSTLIGRLLHDSR
                     QVLEDQLASVTRASARKGIELDLSLLTDGLEAEREQGITIDVAYRYFASAKRKFIIAD
                     TPGHVQYTRNMVTGASTADAAIILIDARKGVLEQTRRHSYIAHLLGVKHILVAVNKMD
                     LVDYAQKVFDTVRSDYLALAERIGIQGVRFFPVSALHGDMVVERGAGMPWHRGRTLLE
                     ALEEIQVHHDAQNKPMRFPVQLVVRPGAGTDFRGYAGRLESGVVRVGDEVVVLPAQRN
                     TRVREIRSLDRSLPFAVAGDSIILLLEDEIDISRGDMIAHHAEAADAPRALKSLTATL
                     CWLSAEGYSPVARYVLKHTSRSVKAKIAAIDHRVDIRSLEKQPAPESLAMNDLAQVQL
                     NLAQPIFADAYAGNRTTGAFILIDEATNQTVAAGMIEA"
     gene            6431..7228
                     /gene="cobA"
                     /locus_tag="VKZ48_00445"
     CDS             6431..7228
                     /gene="cobA"
                     /locus_tag="VKZ48_00445"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012212882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HLU76351.1"
                     /translation="MKTVDRRSLSTHDSRRPTVYLVGAGPGAPDLLTLRAARLLQEAD
                     IVFYDALVHPETVALAQRAEKIAVGKRCGRHSTAQKFINKRLLDAARKYRCIVRLKGG
                     DPMLFGRAQEELDALEAAGIDYEVVPGVTAALAASATIGVSLTRRGLARNVAFVTPRV
                     GETERASDWAAAALHADTAAIYMGAGEAARITATLLARGRAADTPVALVESASLPGTR
                     ARYATLAELPALAAQVGDGPAMILLGEVLREKLALQPATALVQQRSA"
     gene            complement(7274..7645)
                     /locus_tag="VKZ48_00450"
     CDS             complement(7274..7645)
                     /locus_tag="VKZ48_00450"
                     /inference="COORDINATES: protein motif:HMM:NF018228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLU76352.1"
                     /translation="MQKARILVVDDNRDAVESLQMLLQMIGHEVKVAYDGPQAVDAYK
                     AERPDIVFLDIGLPRLNGYEVARKIRGQATGRSPMLIALTGWGQEEDQRLAEQAGFDH
                     HIVKPVAYEQLTDLLRRATGN"
     gene            complement(7986..8678)
                     /locus_tag="VKZ48_00455"
     CDS             complement(7986..8678)
                     /locus_tag="VKZ48_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HLU76353.1"
                     /translation="MEPIYQEISLRLLAALSVGAVIGLERSYRGRPAGFRTHALVCLS
                     TALLMLVTVYESRWVPGIEAGRLALDPTRMAQGIMTGIGFLGAGVIMKEGLSVRGLTT
                     AASIWITAAIGILIGIGFYFPAAVATLLTIGTLSVFHWVENRLPSQHYAQFLVRFAND
                     AVMPEAELRRLVAAHGFSIANLNFRMNVEDGFYEYRMVIRSTNAADAGRLAESLAGVP
                     TVREFRLSPVGD"
     gene            complement(8770..9258)
                     /locus_tag="VKZ48_00460"
     CDS             complement(8770..9258)
                     /locus_tag="VKZ48_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76354.1"
                     /translation="MLVRTIVAIALAYTGLAFAGNAHAWKEVGLKGPGLSRDAQYSGR
                     VLSREELRACVMRRDGLNAAVEQMHEEDVKWTKEAAAIRQLKEEIELAEPMVDRYDPE
                     AVGRYNAMIDEAAKRTAAHHEGMKALGARYYEHKRLRSTFYQQCGARYYEDDLSALEA
                     VR"
     gene            complement(9396..9923)
                     /locus_tag="VKZ48_00465"
     CDS             complement(9396..9923)
                     /locus_tag="VKZ48_00465"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HLU76355.1"
                     /translation="MMKNTRVLSTLLIAGSLALTIPFAAQARPGGMDGCHHAKAVKHG
                     EFGKRGDFARRGGAFMHGLDLTEAQRDRIFEVRHAAAPTLREKGKEAYAARKALREAS
                     KPGAFDEAKVREASAKLAQAQADLTVERLRVQQQMYAVLTPEQRTKVEQAKERRGKHF
                     HGQHHLKGERPAQQS"
     gene            10194..10877
                     /locus_tag="VKZ48_00470"
     CDS             10194..10877
                     /locus_tag="VKZ48_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLU76356.1"
                     /translation="MNKVLLVDDDAELGGMLREYLQQENFDAVHVQDGEQGVKEALSG
                     RYALVVLDVMMPRLGGLEVLRRIRAQSQLPVIMLTAKGDDLDRVLGLELGADDYVPKP
                     CTPRELVARVRAVLRRAQGAGADAPQRIDAGPLVVWPQRRSATWHDVALSLTSTEYNL
                     LEVLARNAGQPVSKADLSQQALGRPLARFDRSIDVHMSSIRQKLSQQGADAGLIQTVR
                     GIGYQLLRT"
     gene            10881..12233
                     /locus_tag="VKZ48_00475"
     CDS             10881..12233
                     /locus_tag="VKZ48_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLU76357.1"
                     /translation="MGRLFWKLFFGFWLTLLLTGLAVATAVNLLRQTQQQEEAPAVAA
                     GPAAVMLVDAAASVLQHGGVEALRSLLRNDEGRFRFARLHAIDAQGNELLGREVSPEA
                     QNAAAALAAQGRARQAARWIEADGTRYLLFVPPSERRAGPPRHRAQPLLPPAMLMLIA
                     GVAASIAFAALLAWYLSRPIRHLRWAFANAAQGKLDVRVQSRIGRRRDEIADLGRDFD
                     HMAQRLQQLLEGQQRLLHDVSHELRSPLARLQAAIGLSRQNPARADAMLERVEEESER
                     LDRLVGELLTLSRLDTATGQDRVEEVDVMEMLEAIAADARFEAQSSGRDLHCTTGPPS
                     IGRFHVDALQRGIENIVRNAVKYTAPGTTVEVVAQRLPDTKQLCIEVCDRGPGVPENE
                     LPRIFEPFHRGEGSSGVSGFGLGLAIAKRAIEADGGTLHARNREGGGLCMRITLPCID
                     "
     gene            12325..12816
                     /locus_tag="VKZ48_00480"
     CDS             12325..12816
                     /locus_tag="VKZ48_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HLU76358.1"
                     /translation="MSRMPRLLALLALLSSPALAQQVTYEARSLTPEAALKVASAALA
                     YCNKQGYQAAVAVTDRSGNALVMLRDRLAGAHTVQTSIDKAYTAASFRMDTADLARET
                     EKGAVSGIRHLPRVTALGGGLPIEAAGTLVGAIGVSGAPGGDADAACAKAGIDAIRDE
                     LEF"
     gene            complement(12900..13994)
                     /locus_tag="VKZ48_00485"
     CDS             complement(12900..13994)
                     /locus_tag="VKZ48_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HLU76359.1"
                     /translation="MSISVRNITKRFNTFTALDNVSLEFSTGELVALLGPSGCGKTTL
                     LRVIAGLERADAGQILFQGKDASRQDVRERQVGFVFQHYALFRHMTVYDNVAFGLQVK
                     PRGQRLSKSEIDDKVHSLLKLVQLDWCADRRPAQLSGGQRQRIALARALAVEPKVLLL
                     DEPFGALDAKVRKELRRWLRRLHDEIHVTSIFVTHDQEEALEVADRVVLMNAGRVEQE
                     GAPEEVYERPATPFVYGFLGNVNLFQGRVHEGWLHVGDDVALETPEYDAAHDTPAVGF
                     ARPHELDIKRYAPGLPGIRVQLSRALVVGPTARLELEREDGVGVVEAEIPTEQYRAMR
                     YYEGETLIVQPKRLQVFLNPKQREEEARAA"
     gene            complement(13991..14896)
                     /gene="cysW"
                     /locus_tag="VKZ48_00490"
     CDS             complement(13991..14896)
                     /gene="cysW"
                     /locus_tag="VKZ48_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HLU76360.1"
                     /translation="MSVVITAPIAAQPAPIRRRSATTEHPLIRWLLTGIALAFLALFL
                     LMPLVAVFYEALSKGFAAYLAALIEPDALAAIRLTLIAAAIAVPLNLVFGVAAAWAIA
                     KFEFRGKSLLTTFIDLPFSVSPVIAGLIYVLMFGAHGWLGPWLQEHDIKIIFAVPGIV
                     LATVFVTFPFIARELIPLMEAQGSEEEEAARALGASGWQILWRVTLPNIKWGLLYGVI
                     LCNARAMGEFGAVSVVSGHIRGLTNTMPLHIEILYNEYQFAAAFAVASLLALLALVTL
                     GLKSLVEWRQHNEQRASGHMEGPQS"
     gene            complement(15028..15870)
                     /gene="cysT"
                     /locus_tag="VKZ48_00495"
     CDS             complement(15028..15870)
                     /gene="cysT"
                     /locus_tag="VKZ48_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HLU76361.1"
                     /translation="MSTLLLRRHSVLPGFNIALGFTLLYLALIVLVPLSAAFLKAATL
                     TWAQFWEAVTTARVVASYKLSFGAALVGAVINAVFGLLVAWVLVRYSFPGKRFIDALV
                     DLPFALPTAVAGIALTGIYAPNGWMGQWLSQYGIKAAFSPLGVIIALTFIGLPFVVRT
                     VQPVLEDLEPELEEAAASLGATRLQTFVRVIFPALLPALLTGFALAFARAVGEYGSVI
                     FIAGNIPMKTEITPLLIITKLEQYDYTGATALAVVMLVASFALLLAINLLQWWTSRRS
                     AKLH"
     gene            complement(16126..>16378)
                     /locus_tag="VKZ48_00500"
     CDS             complement(16126..>16378)
                     /locus_tag="VKZ48_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011827723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HLU76362.1"
                     /translation="VADKHGTRKVAEAYLAYLYTEEGQEIAAKNYYRPRLQKVADKYA
                     ATFPKVKLFTIDEVFGGWQKAQKKHFADGGVFDQIYGAK"
BASE COUNT         3089 a         5364 c         5100 g         2825 t
ORIGIN      
        1 tgcttttctc tgtgtctctg tgcctctgtg gtgaaggttt taatccgcca tgaaaatcat
       61 caggtccatt cccgaactcc gcgccgcgct gaaaggccgc gaaatcgcct gcgtgcccac
      121 catgggcaat ctgcacgaag gtcatttgca gttgatgcgg ctggcgaagc aggaggcctc
      181 cacggtggtg gcgacgattt tcgtcaaccg gctgcaattc gcaccgcacg aggatttcga
      241 cacctatccg cgcaccttcg aggaggaccg cgccaaactc gaacgcgagg gcgtcgatgt
      301 gctgtttgcc ccggatgagc ggcagatgta tccggaaccg cagggttttc atgtcgagcc
      361 gccggactac gccaatattc tggaaggcga attccgcccc ggtttcttcc gcggcgtcgc
      421 tacagtggtg ctcaaactgc tcaatatcgt gcagcccgac gtggccgtat tcggcaagaa
      481 ggactatcag cagctcatga tcgtgcgcaa catggtgcgg caactgaacc tgtcggtacg
      541 catcgtcgcc ggcgaaaccg cgcgcgcagc cgatggattg gctttatcga gccgtaacgg
      601 atatctatcc acgacagagc gcgcacaggc aatactcctg taccgcgtat tgcaggacac
      661 ggcagcagcc attcaagccg gcaaccggga ctacgccgcg ctcgaaacgg cagcggtgga
      721 acggttgagg caggaaaact ggctgcccga ttatgttgca gtgcggaagc aagccgattt
      781 acaatcccca gccgctgtcg ataggcaatt ggtcatactc gccgcagcca gactgggaac
      841 cacccggctg atcgacaatc tagaggttgc cgccccctga acaggggttt aggtccggcg
      901 cccgcgatac cacggggaga gtgctcggct ttcacaggaa gccggcagta gcttgaacgg
      961 agagcagagc atgcagcgta cgatgctgaa atcgaagatc caccgcgcca ccacgacgca
     1021 ttccgaactg cattacgagg ggtcgtgtgc gatcgacgaa gacctgctgg acgcggcgga
     1081 catccgcgaa tatcagcaaa tcgacatttt caacgtcacc aatggcgagc gcttcaccac
     1141 ttatgccatt cgtgcgcagc gcggctcggg catcatttcc gtgaacggcg cagcggcgcg
     1201 caaagccgcg gtgggcgaca ttctgatcat cgccagctat gccgtgtaca acgaaatcga
     1261 actcgaacgc ttcgagcccg atctcgtcta cgtggacagc cgcaaccgca tcaaacacca
     1321 gggcaagcag attccgctgc agcgcgcctg agcgccgccg gttctgttgc aatggccgcc
     1381 cgagggcggc cattttcatt cgggaagtcg atcaacccgt cggcgcatcc gcgatcggcc
     1441 cggtgggccg ggcttcggca ctccgaccgc cccgcggccc ctcggcgatg atttcgatca
     1501 gccgcttgtt gaacgccggc agatcggccg gtttgcggct ggagaccagc gctccgtcga
     1561 ccgccacttc ctgatcgacc catttcgccc cggcgttgac cagatccgtt ttgagcgacg
     1621 accatgaggt gatggtgcgc cctttcacca cgccggcctc gatcagcgtc cacgggccgt
     1681 ggcagatgac gcccaccggc ttgtctgcat cgaagaaggt tttgatgaac tgcaccgcct
     1741 tcggctgcgt gcgcaactga tcgggattgg ccacgccgcc gggcagtacg agtgcgtcgt
     1801 actcctcggc gcgcgcctgt tccagttgca cgtcgacatg aaagcggtcg gccttgtcga
     1861 aatgcctcca gccctgcact tcgtgttgct cgggtgaaac gatgaacgtc tgcgcgcccg
     1921 ccgcttccaa cgcgcgcttg ggctccgtca gttcggattg ctcgaagccc tcggcgacga
     1981 gtatggcgac cctgattcct tccagcatgc gaacctccgt tgtgtcctcc acatgcgcga
     2041 cgcgcgcatg cgctgatcca acaagggctg cacgtgctgt gccgggtggg gtggcggaat
     2101 tttcaacgcg tggtgaatac acgacggcaa cgaccgccga accatcttcg atcgcggccc
     2161 cggcagagtc ctacaactcg taggactcac cgccgccctg caaagcctgc tgaatcactt
     2221 cgcgcgtcaa ctgcggcgcg aacaactgca gaaagtcgta ggtgtagctg cgcagatagg
     2281 cattgcggcg gatggcgatg cgcgtcgtgc tcgcgcggaa cagatgcccc gcgtcgatcg
     2341 ctttcaatcc gcggtcgcgt tgcgggtcga aggccatggc cgcgatgatg cccacgccca
     2401 ggtccagttc gacgtaggtc ttgatcacgt cggagtcgat cgccgtcagc accacatcga
     2461 agttgagttc gcgcgccgcg aacgcatcgt tgatttgaga gcggccggtg aattcggggc
     2521 tgtaggtgat gatcgggtac tcggccagct tctccagcgt gagacgtccg ctcttgagca
     2581 gcgggtggcc gtcgcgcacc accaccacgt gatgccattg gtagccgggc atggcgagca
     2641 gttcgggata gagcgacagc gattcgctgg cgatgcccac atgcgcgtcg ttgcgcatca
     2701 ccatctgcgc cacctgctga ggattccctt gcaacagcga cagatgcacg cggggatagc
     2761 gctgacggaa cgcgcccacc accttgggca gggcgtagcg cgcctgcgtg tgcgtggcgg
     2821 cgacgatgag gctgcccgaa tcacggtcgg cgaaatcgct gcccacacgc ttcaggttct
     2881 cggcctcgtc gagcacccgc tcgatgatct cgagcacggc cttgcccggc tcggtgaggc
     2941 cgatgaagcg cttcccgcgg cgttcgaaga tcagcacgcc gagttcctcc tccagctctt
     3001 tgatctgctt ggagacgccc ggctgggagg tgtagagcgc gttggcggcc tccgtgaggc
     3061 tcattccacg ccgcacggtt tcgcgcacat agcgaagctg ttggaagttc atatggctat
     3121 atctttatta cgttatgttc tatagacatg cttatttatt cgttctcctt atatctgcgt
     3181 ttaactagga tcgcaaccta aatacactcg ctgcctaaac tggccgtttg atctcacgta
     3241 gagcgactgc gcagttcctc gcaacgccgc acaaacgccg tcggcagcag cgccgtacaa
     3301 acgccatgac cgccatttcc gactccatca ccatcgacgc cgacccgggc gaacgcagtc
     3361 aggccctggt cgacatgttg caacagatcg cgcacgacta cgcacccgcc gcactcgcca
     3421 gcagcctgtc catcgaggac atggtcgtca ccgacgccat cctccgtcac gatatcccgc
     3481 tggagatctt cacgctcgac accgggcgca tgcatcagga cacgctcgat ctgatcgaca
     3541 ccatcgagcg ccattacggc acgcgcatcc gcgtctacct gcccgacccc aaagcggtga
     3601 tgcgctatgt cgcgcagcac ggccgcgacg ccttctacga aagcgtcgag ttgcgccggc
     3661 aatgctgcgc catccgcaag accgagccgc tcaagcgcgc gctggcgggc aagcgggcgt
     3721 ggatcacggg cttgcgacgc tcgcaatcgg tcacgcgcgc cgcgctgccg ctgcaggaat
     3781 acgacacgac ccatggattg gagaaattca acccgcttgc cgagtggagc gagcaggagg
     3841 tgtgggctta cctctatgcc aacgacgtgc cctacaaccg tctctatgaa cagggctatc
     3901 gctccatcgg ctgcgccccc tgcacccggc cgaccgtgac cggcgaggac gtgcgcgccg
     3961 gccgctggtg gtgggagcaa cccgaaacga aggagtgcgg gctccatgta gggcccgatg
     4021 gacgtctggt ccgcagcaaa ggaaaggcat catgagcgca gtactgcaag agcgacaagt
     4081 agccgaacta ccgacacgca gccatctgga ctggctggag tcggaagcga tccacatcat
     4141 gcgcgaactc gccggcgagt gcgccaaccc cgtcgtgttc ttctccggcg gcaaggattc
     4201 catcgtgctg ctgcggttgg ccgagaaagc cttccgcccg gggcgcttcc ccttccccat
     4261 gctgcacatc gacaccgggc acaattatcc ggaggtgatc gcctttcgcg atcgacgagc
     4321 ggaagaactg ggcgagcgcc tcatcgtccg ctcggtggag gattccatcc ggcgcggcac
     4381 cgtacgcctg cgctccgaaa ccgaaagccg caacgccgcg caggcggtga cgctgctgga
     4441 ggcgatcgcc gaattcggct tcgacgcctg catcggcggc gcacgacgcg acgaagagaa
     4501 agcgcgcgcc aaggaacgcg tgttctcctt ccgcgacgaa ttcggccagt gggacccgaa
     4561 gaatcagcgg ccggagttgt ggagcctcta caacacgcgc gtgcataagg gcgagcacat
     4621 gcgcgtgttc ccgatcagca actggaccga actggacgtg tggcagtaca tcgcgcgaga
     4681 aaagctcgaa gtgccgtcca tctactacgc ccaccagcgc cccgtcgtac gccgcaacgg
     4741 catgctcgtg cccgtcaccc gcctcacgcc cgcgcgcgaa ggcgagcgca tcgaggagtt
     4801 gtcggtgcgc tttcgcaccg tcggcgacat ctcctgcacc tgcccggtgg aatccactgc
     4861 gcgcgacccc gcagaaatca ttgccgagac cgccatcacc accatcaccg agcgcggcgc
     4921 cacccgcatg gacgacaaga cgtccgacgc ctcgatggaa cggcgcaagc gggaaggcta
     4981 cttctgacga ccttgaagtc ttgaaccacc aaggcaccaa gacaccaagc ttccgatccg
     5041 tttctcttgg tgtttgcttg gtgccttggt gtcttggtgg tgaaggttct aacgattttg
     5101 attcaggaac gaacaatgtc agtacttgga gaactcgaac gcggccggct gaacgccgag
     5161 gatcgcggcg ttttgcgatt catcaccgcc ggcagcgtgg acgacggcaa gagcaccctc
     5221 atcggccgtc tcctgcacga ctcgcgtcag gtgctcgaag accagctcgc ctcggtcacg
     5281 cgcgcctcgg cacgcaaggg catcgagctg gatctgtccc tgctcaccga cggcctggag
     5341 gccgaacgcg aacagggcat taccatcgac gtggcgtatc gctacttcgc ctcggccaaa
     5401 cgcaagttca tcatcgccga cacgccggga cacgtgcagt acacccgcaa catggtgacc
     5461 ggtgcttcca ctgcggacgc tgcaatcatt ctgatcgacg cgcgcaaggg cgtgctggaa
     5521 caaacgcgcc gccacagcta tatcgctcac ctgctcgggg tgaagcacat cctcgtggcg
     5581 gtgaacaaga tggacttggt cgactacgcg caaaaggtgt tcgacaccgt ccgcagcgac
     5641 tacctcgcgc ttgcggaacg catcggcatt caaggcgtgc gcttcttccc cgtctccgca
     5701 ctgcatggcg acatggtggt ggaacgtggc gccggcatgc cctggcaccg aggccgcacg
     5761 ctgctcgagg cgctggagga aatccaggtc caccacgacg cgcagaacaa gccgatgcgc
     5821 tttccggtgc aactggtggt gcgacccggg gcaggaacgg atttccgcgg ctatgccggc
     5881 cgcctggaat cgggcgtcgt acgcgtcggc gacgaagtcg tcgtgctgcc cgcacaacgc
     5941 aacacgcgcg tacgcgaaat ccgcagcctc gatcgatcac tgcccttcgc tgtggcgggc
     6001 gactcgatca tcctattgct ggaggacgag atcgacatct cgcgcggcga catgatcgcg
     6061 caccatgccg aggcagccga tgcgccacgc gcactcaaat cgctcaccgc aacgctctgc
     6121 tggctgtccg cagagggcta tagcccggtg gcacgctacg tgctcaaaca cacctcgcgt
     6181 agcgtgaagg cgaagatcgc cgcgatcgat caccgcgtcg acatccgcag cctggagaag
     6241 cagccggcgc ccgaaagcct ggccatgaac gatctggcgc aagtgcaact caacctggcg
     6301 caaccgatct tcgccgacgc ctacgccggg aatcgcacca cgggcgcgtt catcctcatt
     6361 gatgaggcga cgaaccagac cgtggctgcg ggaatgatcg aagcctagtg gagagtcgag
     6421 cgtggagagc atgaagaccg tcgatcgcag gtcgctctcg actcacgact ctcgacgccc
     6481 gaccgtttat ctcgtcggcg ccgggcccgg cgcgcccgat ctgctgacgt tgcgcgccgc
     6541 gcgactgctg caggaagcgg acatcgtgtt ctacgacgcg ctcgtccacc ccgaaaccgt
     6601 cgcgctcgcg cagcgcgcgg agaagatcgc ggtgggcaaa cgctgcggcc gccattccac
     6661 cgcacagaag ttcatcaaca agcgcctgct cgacgcggca cgcaaatacc ggtgcatcgt
     6721 gcgactcaag ggcggcgacc cgatgctgtt cggacgcgcg caggaagaac tggatgcgct
     6781 tgaggccgca ggcatcgatt acgaagtcgt gccgggcgtg accgccgcgc tcgccgccag
     6841 cgcgaccatc ggtgtctcgc tgacacgccg cggcctcgcc cgcaacgtcg ccttcgttac
     6901 gccgcgcgtg ggcgaaacgg aacgcgccag cgactgggcc gcggcggcgc tccatgcgga
     6961 caccgcagcc atctatatgg gcgcgggcga agcggcgcgc attaccgcca ccctgctcgc
     7021 gcgcggccgc gcagccgata cgcccgtcgc attggtggaa agcgcttcgt tgcccggcac
     7081 acgcgcacgc tacgccacgc tcgccgaatt gccggcactt gccgcgcagg tgggtgacgg
     7141 cccggcgatg atcctgttgg gagaggtgtt gcgggagaag ttggcgctgc aaccggccac
     7201 cgcattggtg cagcaacgga gcgcctgacg cttcgacacg gtcgcacatt aggaccgtcg
     7261 cgtcggtgcc cgatcaattt ccggtcgcgc gccgcaacag atccgtgagt tgttcgtagg
     7321 cgaccggctt cacgatgtga tggtcgaagc cggcctgctc ggccaagcgt tgatcctctt
     7381 cctgacccca gccggtgagt gcaatcagca tcgggctgcg gccggtcgcc tgtccgcgta
     7441 tcttgcgcgc cacctcatag ccgttcaggc gcggcaggcc gatgtccaga aagacgatat
     7501 cgggccgttc cgccttgtat gcatcgacgg cctgcgggcc gtcgtaagcg actttgacct
     7561 cgtgcccgat catctgcaac agcatctgca gcgattcgac ggcatcgcga ttgtcatcga
     7621 caaccagtat tcgcgccttt tgcatagcgt tcacaaatac ggcctcgttg ctttgaatgc
     7681 cggaggaagc gcccggcagc gatgctgcgg aagtgcatcc ccgaaccgca gcgatgcgag
     7741 aatgaatggt acgagcctgt gagataaata gccagaacaa ggcagcgccg caccggctca
     7801 ctgcaaaaaa aagaccgggc aggagcccgg tcgaatttct atcgcaagga ggttagctac
     7861 gtcggccgtt gggcgaccga cgctgcatta ttggaaggct cctccgcata aggaacgaag
     7921 cattggttat atccatatag cccgaaggct tgttcgtccc gaatgttcaa ctttgcgaca
     7981 cgcgcctagt cgccgaccgg tgacaaacgg aactcgcgca cggtcggcac tcccgccaac
     8041 gactccgcca gacggccggc gtctgcggcg ttggtggagc ggatcaccat gcggtattcg
     8101 taaaagccat cttcgacgtt catgcggaag ttcaggttgg cgatactgaa gccatgagcc
     8161 gcaacgagcc ggcgcaactc cgcttcgggc atgactgcat cgttcgcgaa acgcaccagg
     8221 aactgcgcgt aatgctgaga cggcaagcga ttctcgaccc aatggaacac ggacaacgtg
     8281 ccgatggtaa gcagcgtcgc caccgccgcc ggaaaataga atccgatgcc gatcaggatg
     8341 ccgatcgccg cggtaatcca gatggaggcc gccgtggtca ggccacgcac cgacaggcct
     8401 tccttcatga ttacgcccgc acccagaaaa ccgatgccgg tcatgatgcc ctgagccata
     8461 cgcgtcggat cgagcgccag ccggccggcc tcgataccgg gcacccaccg gctttcgtac
     8521 acggtgacca gcatcagcag cgccgtcgac agacaaacca gcgcgtgggt gcggaagccc
     8581 gccggccgtc cgcgataact gcgctccaag ccgatcacgg cgcctacact cagcgccgcc
     8641 agcaacctta aactgatttc ctgatagatc ggttccatgt aactgcgcag tgccttatgc
     8701 ggggtctgta gcgcgtaatg cgactcgaac gtcaacgcat gcgagcggca tgacgagcaa
     8761 ccgccgcgcc tagcgtacgg cctcgagcgc gctcagatcg tcctcgtaat aacgtgcgcc
     8821 gcattgctga taaaaggtcg agcgcaagcg cttatgctcg taatagcgtg cgcccagcgc
     8881 cttcatgcct tcgtgatgcg cggcggtgcg cttggcggct tcgtcgatca tcgcgttgta
     8941 acggccgacc gcttcgggat cgtaacgatc caccatcggc tcggccagtt cgatctcctc
     9001 cttcaactga cggatcgccg ccgcctcctt cgtccacttg acgtcctctt catgcatctg
     9061 ttcgaccgcc gcattcaagc cgtcgcggcg catcacgcag gcgcgcagtt cctcacgact
     9121 gagcacgcgg ccgctgtatt gcgcgtcgcg cgacaggccc gggcctttca acccgacttc
     9181 cttccaggcg tgtgcattgc cggcgaacgc cagtccggtg tatgcgagcg cgatggcgac
     9241 gatggtgcgt acaagcatga tgatctcctc ctggcgcgcc atctaatgcc accgacatac
     9301 gacggtcaag cctcaagggc accgctgtgc gaactatgcg cgcccaacga aaaacggccg
     9361 cccgagggca gccgtttccc gttggcgcgt cacgcttagc tctgctgcgc gggacgctcg
     9421 cctttcaggt gatgctgacc gtggaagtgc ttgccgcggc gctctttggc ctgctcgacc
     9481 ttggtgcgct gttccggggt cagcaccgcg tacatctgct gctggacgcg cagccgttcg
     9541 acggtcaggt ccgcctgcgc ctgcgcaagc ttggcgctcg cctcacgtac cttggcttcg
     9601 tcgaaggcgc cgggcttgct cgcctcgcgc agcgccttgc gtgccgcata cgcttccttg
     9661 cccttctcgc gcaaagtcgg cgcggccgcg tgacgcactt cgaaaatccg gtcgcgttgc
     9721 gcctcggtca gatcgaggcc gtgcatgaat gcgccgccgc gccgggcgaa atcaccgcgc
     9781 ttgccgaact cgccgtgctt gaccgccttg gcgtgatggc aaccgtccat gccgccgggg
     9841 cgcgcttgtg cggcgaaggg aatcgtcagt gcaaggctgc cggcgatcaa cagggtgctc
     9901 agtactcggg tgttcttcat catgtgctcc gttgcgttcg cagggactac ctgcatggcc
     9961 cgaactgtag cgagcgatcg cgtaagcctc ggttaggccc gtgtaaagat aggttaagac
    10021 gggtaaccga ccgtaagcag tgggccgggc gacacacgct ccggctaaac taaggccgtg
    10081 ccggccggaa tgccccgcga atgcgcaaag cgtcacgagc gaagacattg ccgcccgagc
    10141 gacacgaacc tgcaacctat aaaaacctca accggattcc tcctggcacc gcgatgaaca
    10201 aggtcctgct ggtcgacgac gacgccgaac tcggcggcat gctgcgcgag tacctgcagc
    10261 aggagaattt cgacgccgtg cacgtgcagg acggcgagca aggcgtgaaa gaggccttga
    10321 gcggccgata cgccctcgtc gtgctcgatg tgatgatgcc gcgcctgggc ggcctggaag
    10381 tgttgcgccg catccgcgca caaagccagt tgcccgtcat catgctcacc gccaagggcg
    10441 acgatctcga ccgcgtgctg ggactcgaac tgggtgcgga cgattacgtg cccaagccct
    10501 gcacgccacg tgaactggtc gcgcgcgtgc gcgccgtgct gcgccgcgcc cagggagcgg
    10561 gcgccgacgc accgcagcgc atcgacgcag gtccgctggt cgtgtggccg caacggcgca
    10621 gcgccacctg gcacgacgtg gcgctgtcgc tcaccagcac cgaatacaac ctgctcgaag
    10681 tcctggcgcg caacgccggc cagccggtca gcaaggcaga tctctcgcag caggcgctgg
    10741 ggcggccgct cgcgcgcttc gaccgcagca tcgacgtgca catgagcagc atccgccaaa
    10801 agctctcgca gcagggtgcg gacgccggac ttatccagac ggtgcgcggc atcggctacc
    10861 aactgctgcg gacctagcgc atgggccgcc tgttctggaa actcttcttc ggcttctggc
    10921 tgacgctgct gctcaccgga ctggccgtcg ccaccgccgt gaatctgctg cgccagacgc
    10981 aacagcagga ggaagcgccg gcggtcgcgg cagggccggc cgcggtgatg ctggtggacg
    11041 ccgccgcatc ggtcctgcag cacggcggcg tcgaggcatt gcgcagcctg ttgcgcaacg
    11101 acgaagggcg cttccgcttt gcgcgactgc atgccatcga cgcgcaagga aacgagttgc
    11161 tcggtcgcga ggtgtcgccc gaggcgcaga atgccgccgc ggcgctcgct gcccagggac
    11221 gagcgcgaca ggccgcgcgc tggatcgaag cggacggcac gcgttatctg ctgttcgttc
    11281 cgccctcgga gcgacgcgcc ggcccgcccc gtcatcgtgc gcaaccgctg ctgccgccgg
    11341 caatgttgat gctcatcgca ggcgtcgcgg cgagcattgc cttcgccgcg ctcctcgcgt
    11401 ggtatctctc acgccccatc cgccatctgc gctgggcctt cgccaacgcg gcgcagggca
    11461 aactcgacgt gcgcgtgcag tcgcgcatcg gccgccggcg cgacgaaatc gccgacctcg
    11521 ggcgcgattt cgaccatatg gcgcagcgcc tgcagcaatt gctggaagga cagcagcggc
    11581 tgctgcacga tgtgtcgcac gaactgcgct cgccgctggc gcgcctgcag gcggccatcg
    11641 gcctgtcgcg ccagaatccg gcgcgcgccg acgcgatgct cgaacgcgtg gaggaggaat
    11701 cggagcgcct cgaccggctg gtgggtgagt tgctcacgct ttcccggctt gacacggcaa
    11761 cgggtcagga ccgtgtcgag gaagtcgacg tgatggaaat gctggaggcg attgccgccg
    11821 acgcgcgctt cgaagcgcaa tcgagcggcc gcgatctgca ctgtacgacc ggtccgccgt
    11881 ccatcggccg ctttcacgtc gacgccttgc agcgcggcat cgagaacatc gtacgcaacg
    11941 cggtcaaata taccgcaccg ggcacgacgg tcgaagtcgt cgcgcaacgc ctgcccgaca
    12001 cgaagcaact gtgcatcgag gtgtgcgatc gcggtccggg cgtgccggag aacgaactgc
    12061 cccgcatctt cgagcccttt catcgcggcg aaggcagcag cggcgtcagc ggcttcggcc
    12121 tggggctggc catcgccaag cgcgccatcg aggccgacgg cggaacgctg catgcgcgca
    12181 atcgcgaagg cggcggactg tgcatgcgca tcaccctgcc ctgcatcgat taggtgcgcg
    12241 acgcagcggg cggcagggtt gatcgcaacg ttcctcgccc ggcatcattg caggccaccg
    12301 taccgcaacg cccaggagaa ccgaatgtcc cgcatgcccc ggctgcttgc gctgctcgcg
    12361 ctgctatcct cccccgcgct cgcgcaacaa gtcacctacg aagcgcgcag cctcacgccc
    12421 gaagccgcac tgaaagtcgc ctcggctgca ctggcctact gcaacaagca gggttaccag
    12481 gctgccgttg ccgtgaccga ccgctcgggc aatgcgctgg tcatgctgcg cgaccgcctg
    12541 gccggcgcgc acaccgtgca gacttccatc gataaggcgt acacggccgc gagcttcagg
    12601 atggacaccg ccgacctggc gcgcgaaacg gaaaagggcg cagtctccgg catccgtcat
    12661 ctgccgcgcg tgacggcgct cggcggcggc ctgccgatcg aggcggccgg cacactggtc
    12721 ggcgccatcg gcgtgtccgg tgcgccgggt ggcgacgcgg atgccgcgtg tgccaaagcg
    12781 gggatcgacg cgatccgcga cgaactggag ttctagaaaa gcagtcgaga gcggagagtg
    12841 aagagtagag tctttcggtt ttgctctcca ctctcgactc ataactctcc gcttcctact
    12901 cacgccgcgc gcgcctcttc ctccctttgc ttgggattga gaaagacctg gaggcgcttt
    12961 ggctgcacga tcagcgtttc gccctcgtag tagcgcatcg cgcggtattg ctcggtggga
    13021 atttccgctt cgacgacgcc cacaccgtct tcccgttcga gctcgagtcg cgccgtgggg
    13081 ccaaccacca gtgcgcggct caattgcacg cggatgccgg gcaggcccgg cgcatagcgt
    13141 ttgatgtcca gttcgtgcgg ccgcgcgaag ccgacggcgg gcgtatcgtg cgccgcatcg
    13201 tattccggcg tctcgagcgc cacatcgtcg ccgacatgca gccagccttc gtgcacgcgt
    13261 ccctggaaca gattgacgtt gccgaggaaa ccatagacga aaggcgtcgc aggccgctcg
    13321 taaacctctt ccggcgcacc ctcctgttcg acgcggccgg cattcatcaa caccacgcgg
    13381 tcggcgacct cgagcgcctc ttcctgatcg tgcgtgacga aaatgctggt cacatggatc
    13441 tcgtcatgca accgtcgcaa ccagcggcgc agttccttgc gcactttggc atcgagcgca
    13501 ccgaacggct cgtcgagcag cagcaccttc ggctccaccg ccagggcgcg cgccagcgcg
    13561 atgcgctgac gctgaccgcc cgacaattgc gccgggcgac ggtcggcgca ccagtcgagt
    13621 tgcaccagct tcaatagcga gtgcaccttg tcgtcgattt cggacttgct gagacgctgg
    13681 ccacgcggct tgacctgcag gccgaaagcg acgttgtcgt acaccgtcat gtggcggaac
    13741 agcgcgtaat gctggaacac gaaaccgacc tggcgttcgc gcacgtcctg ccgcgatgcg
    13801 tccttgccct gaaagaggat ctggcccgcg tcggcccgct cgagacctgc gatcacgcgc
    13861 agcagcgtgg tcttgccgca accggacggt cccagcaatg cgaccagctc tccggtggaa
    13921 aactccagcg agacgttgtc gagcgcagtg aaggtattga agcgcttggt gatgttcctg
    13981 acggagatgc tcatgactgc ggtccttcca tgtgtccgct cgcgcgctgt tcgttgtgct
    14041 gccgccactc gaccagactc ttcaacccga gcgtcaccag cgcgagcagc gcaagcagcg
    14101 acgcaacggc aaaagcggcg gcgaattggt actcgttgta cagaatctcg atatgcagcg
    14161 gcatggtgtt ggtcagcccg cggatgtggc cggagacgac cgacacggcg ccgaactcgc
    14221 ccatcgcgcg cgcattgcag aggatgacgc cgtagagcaa tccccacttg atgttgggca
    14281 gggtcacgcg ccagagaatc tgccagcccg atgcgcccag cgcgcgcgcg gcctcttctt
    14341 cctcgctgcc ctgcgcttcc atgagcggaa tgagctcgcg tgcgatgaac ggaaaagtga
    14401 cgaataccgt cgccagcacg atgcccggca cggcgaagat gatcttgatg tcgtgttcct
    14461 gcagccaggg gccgagccag ccatgcgcgc cgaacatgag cacatagatc aggccggcga
    14521 tcaccggcga taccgaaaac ggcaaatcga tgaaggtcgt aagcaggctc ttgccgcgga
    14581 actcgaattt cgcgatcgcc caggcggcgg cgacgccgaa caccagattg agcggcacgg
    14641 cgatggcggc agcgatgagc gtaagcctga tggcggccag cgcatcgggt tcgatcagcg
    14701 cagccagata ggcggcgaaa cctttgctga gcgcttcgta gaacaccgca accagcggca
    14761 tgagcaggaa cagcgcaaga aaggcgagcg cgatgccggt gagcagccag cggatcagcg
    14821 gatgttccgt ggtggcgctg cggcgccgga tcggcgcggg ctgggcagcg atgggagcgg
    14881 taatgacgac ggacactgga atagcctcgt taagcgccga agcgggtcgc gggtgcgcga
    14941 aggcctgaga gcaaggacct gattcccggc cgctcattcg gcgccgcgcg cgacaggatt
    15001 tccgcaagag ctttcatcgt gcgtcgttca atgcagtttt gcgctgcggc gggaggtcca
    15061 ccattgcaac aggttgatgg cgagcagcag ggcgaacgac gcaaccagca tgacgacggc
    15121 cagtgcggtc gcacccgtgt agtcgtactg ctccagtttg gtgatgatca gcagcggcgt
    15181 gatttcggtt ttcatcggaa tgtttccggc aatgaaaatg accgagccgt attcgccgac
    15241 cgcacgggcg aaggcgagcg cgaagcctgt cagcagcgcc ggcagcaggg ccgggaagat
    15301 cacccggacg aaagtctgca ggcgggtcgc gcccagactc gccgccgctt cttccagttc
    15361 gggctccagg tcttcgagca ccggctgcac cgtgcgcacg acgaaaggca ggccgatgaa
    15421 cgtcagcgcg atgatcaccc cgagcggact gaacgccgcc ttgatgccgt actgcgacag
    15481 ccattgaccc atccagccgt tgggcgcata gatgccggtc agcgcgatgc cggccaccgc
    15541 cgtcggcagc gcaaagggca gatcgaccag cgcatcgatg aaacgcttgc ccggaaagct
    15601 gtagcgcacc agcacccagg cgaccagcag gccgaacacg gcattgatta ccgcgccgac
    15661 cagcgcggcg ccgaaggaca acttgtatga agcgacgacg cgcgcggtcg tcaccgcctc
    15721 ccagaactgc gcccaggtca gggtagcggc cttgagaaac gctgccgaca gcggaaccag
    15781 cacgatcagc gccagataga gcagcgtaaa gccgagcgcg atattgaacc cgggcagcac
    15841 gctgtggcgg cgcaacagca acgtgctcac cttaccgccc ccgttttgtc gtgctctaaa
    15901 ctcgcgtggg caacgcggtg cacgagggtg cgggacgccg gggctttcga catggaatgc
    15961 atggagcaga gcgaagcggc agcggtgccg tcgccggttc gattcttctg agctttgcga
    16021 tgacgcattt tgtacaaagg cgtttattac gtgaacgaat ggatcgccat ttcgttataa
    16081 gtcacagcga tatcgcaggc cgccaggcga cctgcgacaa gtcgcctact tcgcgccgta
    16141 gatctgatcg aacacgccgc cgtcggcgaa gtgctttttc tgcgctttct gccagccgcc
    16201 gaacacctcg tcgatggtga acagcttcac tttgggaaag gtcgccgcgt acttgtccgc
    16261 caccttttgc aggcgcggac ggtagtagtt cttcgccgcg atctcctgcc cttcctccgt
    16321 gtagaggtac gccagatagg cctccgcgac tttgcgcgtg ccatgcttgt ccgccacc
//
LOCUS       DATGOU010000008        31418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_6243197, whole genome shotgun sequence.
ACCESSION   DATGOU010000008 DATGOU010000000
VERSION     DATGOU010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 31418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31418
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6243197"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VKZ48_00505"
     CDS             complement(<1..887)
                     /locus_tag="VKZ48_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HLU76363.1"
                     /translation="MKRKWLVATLFAFAGMTQSVGAQTSLLNVSYDPTRELYQEFNEA
                     FAKHWKAKTGERVNIKQSHGGAGKQARAVIDGLEADVVTLALAYDIDAIAAQGLIAPD
                     WQKRLPQNSAPYTSTIVFLVRKGNPKGIRDWDDLVKPGVSVITPNPKTSGGARWNYLA
                     AWGYALKKPGGSEASAKDFVARLYKNVEVLDSGARGSTTTFVKRQIGDVFISWENEAF
                     LAIEELGPGQFELVVPSVSILAEPPVTVVDKVADKHGTRKVAEAYLAYLYTEEGQEIA
                     AKNYYRPRLQKVADKYAAT"
     gene            complement(1234..1875)
                     /locus_tag="VKZ48_00510"
     CDS             complement(1234..1875)
                     /locus_tag="VKZ48_00510"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003813333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HLU76364.1"
                     /translation="MATLRLGDIAPDFEQDSTVGKIRFHEWLGDSWGVLFSHPADFTP
                     VCTTELGYTAKLAEEFAKRNVKVLALSVDPVDSHHGWVKDINETQSCVVNFPILADAD
                     RKVSELYDMIHPNASATVTVRSVFIIDPQKKVRLTLTYPASTGRNFDEILRVIDSLQL
                     TDSHSVATPVNWKQGEEVIIVPSITDPEVLKQKFPQGFRAVKPYLRYTSLEKK"
     gene            complement(2003..3196)
                     /locus_tag="VKZ48_00515"
     CDS             complement(2003..3196)
                     /locus_tag="VKZ48_00515"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HLU76365.1"
                     /translation="MLKKFLRCIAAGLLGLTLSLSVQAHGDHRHAPAAKVRIDLGASA
                     AFDARSGDLWAVYKQGDHVMLTRSADAGRTWSEAQPVNPKPEKVAADGDARPKIALGS
                     EGEIYVTWTTPLSKPYTGNVRFARSVDAGETFSEPVTVNADRQEITHRFDALAVDREG
                     RIFVAWIDKRDLVAAKAAGNGAYRGAAVYYAVSDDRGASFRGDYKLADHSCECCRIGL
                     YAAADGGVLAMWRHVFEPNVRDHALARMDASGAAGPVRRVTFDDWRIDACPHHGPSVV
                     GDAKGTLHAVWFTAGDRHSGVYYGRLGEGAVDGLRRLGGDAAAHADIAAAGDRVAVAW
                     KEFDGQRTQLRAMVSDDGGRTWREHRLAATDGASGQPMVLEHEGRFHVFWHTRQAPLS
                     VTAIP"
     gene            complement(3223..3720)
                     /locus_tag="VKZ48_00520"
     CDS             complement(3223..3720)
                     /locus_tag="VKZ48_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HLU76366.1"
                     /translation="MKAKRLLRNALLAAALVLYACTAVRAQDQLRPFNTNSLDAIVQQ
                     HAGKPFVLALWSIHCLPCREEMAYWNDLRRRYPQVPIVLVSTDLPEEWSDVRRFLSRY
                     DPGPVERWAFADEYVERLRYAIDRKWHGELPRTYFYDANHVVQGRSGKLDIAWLTDWF
                     NRHAK"
     gene            complement(3728..5881)
                     /locus_tag="VKZ48_00525"
     CDS             complement(3728..5881)
                     /locus_tag="VKZ48_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLU76367.1"
                     /translation="MYAVCRNHIAIGTRVLGGTLLLGAIFADAALNEIHAQEATELLP
                     EVVVSGTREKEALAKTPASVGIIKNEAVEAVKPTHPAQIISQVPGAAVSVTNGEGHTT
                     AIRQPFTTDPVYLFLEDGIPTRSTGFFNHNALYETNLPMAGGVEVIRGPGTALYGSDA
                     IGGIVNVLTREPPMTPELSLTAEGGSHGWWRLLGSGGTGYADGGWRTDLNLTHTDGWR
                     DHTAYDRQSATIRWDHFAGASLVKTLLSYSRIDQDTGANAPLPRADYKHHPKKNNHSI
                     AFRDVEAFRLSTTVEYERGNDLVSLIPYFRHNSMDLLASFRLAAPDPTVYTTENQSFG
                     LMAKWRRDFPGAMNARLIVGVDIDHSPGSRDEDRIFVTTTGPGTGASRNFTGYTVGPK
                     LYDYDVSFTGISPYVHAEISPIERLRLTGGLRYDRLRYDFDNRLADGGVLNVAGRFYG
                     QADDTDVAFNQLTPKLGATWEFNPQTHGFVSYNRGFRAPSENQLFRPGSAATAAQAEA
                     FRDSALDLKAIKTEQIEIGLRGTLSGVSYNLALYNLEKKNDILSFRDTTTNESRTVNA
                     GKTRHRGVELGLGSPLGRHWRADVAFSYAKHKYMDWEVGTDDFSGNEMEAAPRITANT
                     RLTWTPAEAVRLQLEWIRLGSYYMDAANTKKYSGHNLYNLRAHWQVAKQVALYAHVYN
                     LGDTRYADSASISSDTPVYSPGLPRTYYAGVEMKW"
     gene            complement(5990..6370)
                     /locus_tag="VKZ48_00530"
     CDS             complement(5990..6370)
                     /locus_tag="VKZ48_00530"
                     /inference="COORDINATES: protein motif:HMM:NF022610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HLU76368.1"
                     /translation="MSVLSIRRRPPVWIALLAMLWQLLLPWSPALANDGLAGELIPVC
                     TSTGLKWIAPGDSTPTETGVHQDRTHCALCVFGDFHPALHDAREPLAVLAAASSAPRA
                     TDVPAPHTAPPHAIPPSRAPPLHA"
     gene            complement(6498..7559)
                     /locus_tag="VKZ48_00535"
     CDS             complement(6498..7559)
                     /locus_tag="VKZ48_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HLU76369.1"
                     /translation="MAKRIAITLLLFLGFTFAAHAELRKFEMTIEELDLEVAPGFTVS
                     VWAYDGQVPGPLIHVKEGDTVEVTLTNNTTMSHTIHWHGIYQTKTWQMDGVPDVSQKA
                     VEPGDSFTYKFVADKPGTLFYHCHVNTAEHIDLRGMWGPLIVDPKNPTPLEKKVTKDV
                     ILMLSGWASAVAKDYGENGLPGREFDFFSINGKAHPHNQPVRVKKGDIMRLRLIAPNI
                     PVAFHLHGHDSLVTHKDGLPLPKPYYVDVLPMQPGERYDMIVEMNNPGIWMAHDHNEE
                     HTTGAGKEGGGAMLVFEYEGIEHPDFYMWKDHPYDPDFYMTESLKKGYGKHNNPSFKA
                     FDPTEPRPAAKQAAGGHGH"
     gene            complement(7575..7922)
                     /locus_tag="VKZ48_00540"
     CDS             complement(7575..7922)
                     /locus_tag="VKZ48_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLU76370.1"
                     /translation="MRNIILAAAFLATSGLAYAEADHGEQLYQTYCTQCHGVNANGKG
                     INASHMSVQPRDHTDTKEMSARTDDELFKVIKHGGKSINKSILMPAWGGNLNDDDIHA
                     LVAHLRKLCCQKN"
     gene            complement(7925..8611)
                     /locus_tag="VKZ48_00545"
     CDS             complement(7925..8611)
                     /locus_tag="VKZ48_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76371.1"
                     /translation="MKRLYSWGVAALLAAGAPVAAQADVAADVQQQCGSCHALTHDYA
                     AESIAERAARKAPPLDYAGNKFNREWLVSWLQKPEPIRPAGTFPPAHVQAGADGDTIA
                     ADGLTKHPALPAAQAEAVADYLMTLTPFTKLIQAQNYTPGNIALRMGKMNFGKFKGCD
                     ACHQDAPGKGGVSGPELYTAWQRLQPGFISAYIADPVVWDPHTMMPKGDLNDDAVHKL
                     ANYLKAIGEK"
     gene            complement(8638..9579)
                     /locus_tag="VKZ48_00550"
     CDS             complement(8638..9579)
                     /locus_tag="VKZ48_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HLU76372.1"
                     /translation="MNQKPLLSALLLSAALLGTEAIAATVKVEIPVREVDLVIDNAGN
                     KQPMWTYNGTIPGPLVRVKEGDTIDFTLINDPKNKNSHSMDFHAGRFDVLGEFETINP
                     GEKKQFTWKAEHPGVWIYHCGADSMSEHISRGMYGVVIVDPKDGYSDAYPKPDREYVL
                     VHGDLFEAGASAEERTDNLKWKGVLVNGKVFHYDPVHDSNASITLESKPGERVRIYFV
                     NAMINEWAAFHPIAGIWDRVWDNGNPKNVLYGMQTMQVPPAHAVTLDLVSPADRPTNN
                     ALVDHAMKKAHHGGISILMNHKDADPNKGRGDQLILR"
     gene            9739..11280
                     /gene="norR"
                     /locus_tag="VKZ48_00555"
     CDS             9739..11280
                     /gene="norR"
                     /locus_tag="VKZ48_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HLU76373.1"
                     /translation="MTTNAFHNLAAAVADLSKAMTPEVRYTRLLDAFRNSFPCDAIAL
                     LERDGEVLIPRAVAGLSRDTLGRRFVIHDQPRLAQILHSADPIRFPADSALPDPYDGL
                     VEHETGDSLYVHDCMGASLYVDGQPLGVVTLDALRPGHFDEIDYGVFQAFVAVAAATV
                     RAAGWIQRLEAQLERHHLIQRLNVQDGSGEELIGQSLVMQQLQHEAETVAPSDLPVLI
                     LGETGVGKELVARLIHRHSSRAREPMVYINCAALPESIAESELFGHVKGAFSGASDDR
                     MGKFELAHEGTLFLDEVGELPLPVQAKLLRALQNGEIQRVGSDAHHRVDVRVIAATNR
                     DLKKEVTDGRFRADLYHRLSVYPLLVPPLRERGEDVLLLAGYFIERNQRRLGLRAVRL
                     GRGARQWLTQYNWPGNVRELDHTLSRAMIRALSVASRRDRVIELTTQHIGADGARMPH
                     SDGAHIAEAVNDQRPLSEVLDQTRREIIQARLRAHHGNRAAAARSLGLDRGNFHRLLK
                     RMGLN"
     gene            complement(11544..12710)
                     /locus_tag="VKZ48_00560"
     CDS             complement(11544..12710)
                     /locus_tag="VKZ48_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008450527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLU76374.1"
                     /translation="MRIPVSYSLRNLWARKLTTLLTAGGMALVVFVFAAVLMLDAGLR
                     AALVATGQPDNVLVTRRAAATEVQSSIERNQAAIIESNAAVAIGADGRPLASKETVVL
                     ITLPKRDTGVQTNITTRGIGPAGLAVRPQVEIVSGRMLRFGTAEIMVGRGVAERFEGA
                     GLGQRLRFGGREWPVVGVFEAGGAAFDSEVWGDSEQLMQAFRRQAYSSVVARLRSMEA
                     FDDFKAAIENDPRLPVDVKRERVFYEEQSKTMSTFISYLGITLSIIFSIGAMIGAMIT
                     MYAAVANRTAEIGTLRALGFSRSGILGAFLLESLMLSAVGWVVGVALASLMQLVHIST
                     MNWQSFAELAFSFTLTPDIVWRSLVFALLMGFAGGVLPALRAARLRIVDALRAA"
     gene            complement(12726..13889)
                     /locus_tag="VKZ48_00565"
     CDS             complement(12726..13889)
                     /locus_tag="VKZ48_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011546674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLU76375.1"
                     /translation="MHFLKLILRNGLRHKLRSGLTVLGLVVAILSFGLLQTIVDAWYA
                     GADAAAANRLVTRNAVSLVFPMPIHYGQRIRAVDGVTGVAPANWFGGVYRDTSMANFF
                     PRFAVDPVPYFAMYPEYRLDEDEFRTFLRDRKGAIIGRKIAENHGFKIGDVIPIQGDI
                     FPGNWEFTVRAIYDGAQPSTDTSLMFFHYEYLNESLKKRAPARADKVGVFVVSIDDVD
                     RAADIGREIDELFRNSAAETLTETEKAFQIGFAKQTEAIVIAVRFVSYVVIAIILAVM
                     ANTMSMTARERMAEYATLKALGFQPAFVGGLILGESLAIAVAGAALGIGLTFPIGHWF
                     ASQMGTLFPVFGVSRETILLQASCALIVGVVAALLPMRRAAGVKIVEGLRFVG"
     gene            complement(13897..14631)
                     /locus_tag="VKZ48_00570"
     CDS             complement(13897..14631)
                     /locus_tag="VKZ48_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLU76376.1"
                     /translation="MNAADPDTLVRIRHLVKSYSRGGQRVPVLTDINIDIRRGEFLGL
                     MGPSGSGKSTLLNMIAGIDKPDSGEVLIEGVDIARLSEAELAHWRAAHVGFIFQFYNL
                     MPVLNAFENVELPLTLTRLSRRERRERAEMALELVGLADRMAHLPSELSGGQQQRVAI
                     ARAIITDPTLLVADEPTGDLDRASAEDILALLDRLNTELGKTIIMVTHDRRAADQAHL
                     IMYLEKGELSTSADIAERSVRASRHH"
     gene            complement(14628..15842)
                     /locus_tag="VKZ48_00575"
     CDS             complement(14628..15842)
                     /locus_tag="VKZ48_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLU76377.1"
                     /translation="MSASELSRLSIDRRAARPGGPRRVRWGLWLGIAVAVLVAAAILW
                     QRYAGAVEVQTVSVTTAYPAQAITLLNATGYVVAQRKASIASKATGRLEWLGVSEGST
                     VKEGEIIARLENRDVAATREQAEANVKVAQANLEQARAELREAKRAFARSEDLIGKRY
                     ISEAAHDQARARLEVAQAALASHEAALAVARANLEAAQVGFDQTLIRAPFDGVVLTKN
                     ANVGDNITPFSSALDTKGAVVTMADMSTLEVEADVAESSLGQIEVAQPVIIQLDAIPD
                     ERFGGTVNRIVPTVDRTKATVLVKIRFDERDQRVLPDMSAKVAFLERPLRADERAPVI
                     AVPAGAVLQDEQGTAVLRIRDGRAERVAVQTGGKLGDLVQVSGVEPGQRLVAQPLDPA
                     IAGRKVTVAQQP"
     gene            15974..16789
                     /locus_tag="VKZ48_00580"
     CDS             15974..16789
                     /locus_tag="VKZ48_00580"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HLU76378.1"
                     /translation="MQAFMRPLVCALLLGIALPAVAETPDPVLFAVRVEAGDLPAAEQ
                     WLAAGLDPDYEGDRIGSGLMIAAWEGNVPMMALFHRYGADVNRINRNGEQALLHAAWK
                     GRKSAVEWLLAHGAQLDRPGRHWSALHYAAFAGHEDIVRLLIERGADVNALAPNGSTV
                     LMMAAREGHGALAQLLLAHGANPAARNDYREDAVDWALRYGHTQIARTLSSAERVERA
                     VQVAALAPPVVESVPAPRPLAELVEALREARAAGRPTDEVLQRYAVTLDALKH"
     gene            17029..19461
                     /locus_tag="VKZ48_00585"
     CDS             17029..19461
                     /locus_tag="VKZ48_00585"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HLU76379.1"
                     /translation="MPAFRIRWNFAVKAVVSATTGLLFASVFAHTTLYERLDGWLQDS
                     LQRTVSAPIDLSEVLVVDIDDASLSTLLRALGPWPYNRDTFAYIQSYLMQQGARLVVY
                     DLLLTDARDAAPNPLDVPQNIVYAGAGFAVPLPVTPAYRTQLETLAIGRDDRLVDESG
                     VNTGNGYRSWSYLKLPLVDEQTRHAVGVMNIQPDEDGVVRRVALFHGAQGYVLPSLSL
                     AALMAAHPEQVAADWPNRRLRVRRTDLPLTEHGEVLLRYPSNTNELRVVPFYELMFAA
                     TEPDDAMRSTRQLKDRVVFIGSSSFAGGTHAYTPMDRMPGVQLSALTYAMLANGSVLS
                     PPKLAVDGAIAAFALSLPLLLLWRGVESSGRAFFMVFIGLPLLCVGIAIALLMGGQLS
                     NWLYAVAAALAAWATIFAIWLFDIANDRRRLRYEAIAAREANRLKSQFLNQVTHELRT
                     PLTAIMGFNKINQYTEDLGREARIKNSEIIGRNCDHLLALINNNLDLAKIEAGALSIA
                     LAPEDPEQLCRDVVATLQPVADEKRLRLKFTRGTELPAALMLDAFRVRQVLMNLMGNA
                     LKFTQNGTVELTALYHAPTLVLEVRDTGAGIPADALERIFHPYEQADSSVAQRFGGTG
                     LGLAITRTLIELMDGVIEVESEPGVGSTFRVRLPAEVTVRPDTVRPITDARPMEILSG
                     RVLIAEDNEDIRALIALQLGKLGVETVTVPHGLAAVETALADSFDAVLIDMEMPVMNG
                     FEAANVLRTRNYQGTILALTAHQEGMEIERALASGCDGVVRKPASIESLRAALRPVLR
                     GSRRATGSAQAG"
     gene            19474..19842
                     /locus_tag="VKZ48_00590"
     CDS             19474..19842
                     /locus_tag="VKZ48_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HLU76380.1"
                     /translation="MQGLPTHTPDRARGFGTSRSTAPSRRICVRVASELEPLIPRFLA
                     NRRTEIASLEQAVESRDAETLRRIAHGLKGAGGGYGFDEITRIGAQLEGCAKAGDDQG
                     ARPLIHALKRYLDDVEVRFD"
     gene            19865..20617
                     /locus_tag="VKZ48_00595"
     CDS             19865..20617
                     /locus_tag="VKZ48_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013091610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLU76381.1"
                     /translation="MRIALLEDDSAQRDLVKLWLDGAGHICHAYGRSKDFMRILTRDT
                     FDLLLIDWELPDVNGDRVLEWVRGNMREYVPVMFATARSEEDDICAILRAGADDYLVK
                     PLRKNELLARIEALGRRGKPPAHQNEPLRVGDFEIDFECRVLRRGGVPVLLTQKDFDL
                     AVFLFRNVGTLLSRGHILETVWGRNPDLNTRTVDTHISRLRSKLGLTPENGWRLSAVY
                     QQGYRLEPVAERDAPVFARRKPSAPLQIATSP"
     gene            20936..29425
                     /locus_tag="VKZ48_00600"
     CDS             20936..29425
                     /locus_tag="VKZ48_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HLU76382.1"
                     /translation="MNFLPYPRLGASSSPWDNNSPVREELFGIERLEQHAESLAAAQT
                     ITNRPPAVLTLRARLKSNAKALRAAYRIYASELERGHTVVPAADWLLDNFHIVEEQIR
                     EIRDDLPPGYYRQLPKLATGPFAGYPRVFGLAWAFVAHTDSHLDTDILRRYLAAYQRV
                     QPLTIGELWAVAITLRIVLIENLRRLADDITAVLAARAAANALANRLIEADDVRAVLE
                     NDGAYRSSALLSAAFAAHLAKRLREQDPKTMTALEWLEERLGGQGTTIEQVVLHSQQQ
                     QSASNLSVRNVITSMRLISNVDWTELFESVSLVDQRLRAGSAFGAMDFRSRDLYRDAI
                     EDLARGSSRTELEVADLAIGAAQAAASEAPDEAQALRVGDPGYHLIAGGRRAFEQAIG
                     FKSPFRLRLNRLNLRLGIRGYLGAILLTTTLLLALVLQALALPDLDRAWLVLFALVAF
                     LPATEVATALVNRAVTRGFGPRIVPGLELKSGVPESLRTLVAVPTLLVNEDDLLEQIE
                     RLETHFLSGIGGDLTFALLTDGADADSEVVESDARLLALATEAIAELNARYGPGPAGD
                     RFLLLHRRRVFNPAEGKWMGWERKRGKLHELNRLLRGATDTTFQAVDGRPPQVPANVR
                     YVITLDADTRLPRDAAARLVGKMAHPLNRPVFDKTAQRVVDGYAILQPRVTPALPLRR
                     EGTLYQKVFSGPGGLDPYAAAVSDVYQDLFGEGSYTGKGIYDVDAFEAALAGRVPENT
                     LLSHDLFEGIYARAGLVTDIEVFEDFPARYDVAVKRQHRWTRGDWQLLPWIFGLRRGP
                     GGLPSVGRGKMLDNLRRSLLPPFTLLTLGVCWLLPLPASLLGTAIVLAAIAIPPFLPV
                     LFGVMPPRGIRLRNHLRALAGDLRLAAMQSFFALAFLPDQAWRMCDAILRTLVRLGIT
                     RRHLLEWTTAAQVSSRPTPGVEGFYRKMAPGTALGLLIAAGAVLCMPSSWPVALPVVL
                     LWLSAPAVAYSASRTPPAAHQAIAAPTAQALRLVARRTWRFFETFVTPADHMLPPDNF
                     QEDPAPVVARRTSPTNMGLYLLSAVAARDFGWAGTVETVERLEATLDTMVKLPRYNGH
                     FYNWYATADLRPLEPAYVSSVDSGNLAGHLITLANACESWIGEPLAPNARLGVLDTLQ
                     LVRETLGAKLNPDDRHGRQLAALLDEFETQLNGAQWIETLPSLKRYIDKAGRTALEVV
                     PAGEGDRVPEAVYWIEALRKTLAEHARDRLQREDTQALDDRLRSIAARAREMAHAMDF
                     SFLLDRERKLLSIGYSLADNRLDQSCYDLLASEARLASLVAIAKGDVPTQHWFRLGRT
                     ATPVRNGSALISWSGSMFEYLMPSLVMRAPADSLLEQTNRLAVRQQQDYGASLNIPWG
                     MSESAFNARDLEYTYQYSNFGVPALGLKRGLSENIVVAPYATGLAAMIDPYGSVQNYN
                     RIAALGGLGRYGYYEALDFTRSRLHENENVAIVRTYMAHHQGMPIVAIANVIHAGEMR
                     ARFHREPMIQASELLLQERMPRVAPIMPPRAEEISVAAWAEQNEGTAARRLEPAPDDA
                     PVTHLLSNGRYAVMLTASGSGYSRWRDIAVTRWREDATRDDWGSFIFLRDADSGKRWS
                     VGAQPLGWGDGQRSVVFDEDHAEFNYREGALTTTMNVVVSGEDDGEVRRLSLVNSGRQ
                     PRVIEVTSYAEIVLTTPAADEAHPAFAKLFVQTEFLEEFGAIVATRRPRAQNEPPIWA
                     AHFAVVEGEAAAAPQYESDRARFIGRSRTIATATAIVDARPLSNTVGTVLDPVFALRH
                     RVTIPPGGEAHIAFWTVVAPTRETLIDLIDKNHDRHAYERARTLAWTQAQIQLRHLGV
                     KPDEAADFQRFAAPLIYADSRFRAMPETLLRGADAQSALWPHAISGDLPIVLLHIDEM
                     EDMAQVRQLLRAHEYWRMKRLGVDLVIVNERSTSYVQDLQNAIDTAVRTSRSIQRTGP
                     ALARGSIYVLRADLLSVQARAMLRAAARVTLIARRGPLAAQFTRLPRLPAVVPPAPAR
                     ASTHTATTRLQRPELEFYNGLGGFDKNGREYVVVLDADRTTPAPWINVVANPDFGFQV
                     SAEGSAYTWSENSRENQLTPWSNDPVTDPTGEAIYVRDEESLELWSPTAQPIRDGGTY
                     IARHGHGYSRFEHRANGIALDLLQYVPLSDPIKISRLRLRNLSGRRRRLSVTTYAEWV
                     LGTSRGASAPRVLTEIDRATGALFARNPWAMNFGSRIAFADLRGEQSAWTADRTEFLG
                     RNGTLAAPAALARDIPLSGATGAGLDPCAALQRVIELEPDESIEVVAFLGQCETAEDA
                     HALIARYRSADLDAVYDEVVDHWQTLLGTIQVRTPDRAMDIMLNGWLLYQALACRIWA
                     RSAFYQASGAYGFRDQLQDGMALTFARPEATRHHLLRAAGRQFAEGDVQHWWLPHSGQ
                     GVRTRISDDRVWLAYAAATYIAATGDAAVLDEQIPFLEGPAVSQGEHDAFFQPMTAEE
                     SASLFEHCARGLDQCLALTGKLGLPLIGTGDWNDGMNRVGEGGKGESVWLAWLLLRTI
                     ELFAPYADKRDPERARRWRAHAESVRRAIETKAWDGEWYRRATFDDGAWLGSKDSEEC
                     CIDSIAQSWAVLSGAADPDRARKAMASLEQHLIRRDDGIALLFTPPFDRMARDPGYIK
                     SYPPGLRENGGQYSHAAMWVVLAFAKLGEGDKAGELFALLNPINHSRTPEEAERYKVE
                     PYVVAADVYSVAPHAGRGGWTWYTGSAGWMYRAGIEGLLGIRREGATLVIDPCIPADW
                     PGFEATVRIASTRCDIVVENASKRCRDVAHATVDDVPVDCEHGVRVSLNGGTHRILIT
                     L"
     gene            29684..30340
                     /locus_tag="VKZ48_00605"
     CDS             29684..30340
                     /locus_tag="VKZ48_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLU76383.1"
                     /translation="MYRALLASVGASLAVSMAHAQPTVAPDIALGKNLSVTCAACHQP
                     DGSGRDIPQGESWPRLAGMSAHYLAKQMRDFKSGARKSATMQAFAQMLDDAQIAHVSA
                     YYASLPPPASATPAEADAALLERGKRLALQGDWDRYIVPCKSCHGPDNQGAGPSFPGI
                     AGQHAHYISAQLRAWQEGTRANDPLNLMAAIAKRMTAEDIRAVSAWLATQPARAKGAK
                     "
     gene            30337..31377
                     /locus_tag="VKZ48_00610"
     CDS             30337..31377
                     /locus_tag="VKZ48_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018025459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLU76384.1"
                     /translation="MMTTHARRKNFARKRAWLALLLACGIGVNAQADPQSTAERLAAM
                     APDAPAPTAEQYVHIPPTLEDLEASKLHPELKRVIRRGHDIFVNTQQFRDKYVFNNLN
                     CSSCHLGDGRLPFSGPVWPAAVTLPDYRPKNDHVNNLEERIAGCFSYSMNGKPPAYGS
                     DDMIALATYIQWLAKGVPMYQPGSSLYGRGYPRLAQAAEQPDAARGKAIYAEKCAICH
                     GNDGAGYVQNGEVVFPPLWGDDSYNWGAGISRVFTLAAFIKHNMPLGQPSSLSDQEAW
                     DLAQYIDSRERPQDPRYTGNVKETRAKYLDTFHKHTLYGVEVEGKLLGDHNNTGRKNF
                     LKPEVLQPRHYK"
BASE COUNT         5428 a        10541 c         9914 g         5535 t
ORIGIN      
        1 aaggtcgccg cgtacttgtc cgccaccttt tgcaggcgcg gacggtagta gttcttcgcc
       61 gcgatctcct gcccttcctc cgtgtagagg tacgccagat aggcctccgc gactttgcgc
      121 gtgccatgct tgtccgccac cttgtccacc accgtcaccg gcggctcggc gaggatggat
      181 accgacggca ccaccagttc gaactggccg ggaccgagtt cctcgatcgc caggaaggct
      241 tcgttctccc aggagatgaa cacatcgccg atctgccgct tgacgaaggt ggtcgtcgag
      301 ccgcgcgcgc ccgaatccag gacttcgacg ttcttgtaca ggcgggccac gaagtccttg
      361 gcggacgctt cgctgccgcc gggcttcttc agcgcatagc cccaggccgc caagtagttc
      421 cagcgcgcac cgccggaggt cttgggattc ggggtaatga ccgacacgcc cggcttgacc
      481 agatcgtccc aatcccggat gcctttggga ttgcccttac ggaccaggaa cacgatagtc
      541 gaggtatagg gggcgctgtt ctgcggcaga cgcttctgcc agtcgggcgc gatcaggccc
      601 tgtgccgcaa tggcgtcgat gtcgtaagcc agcgccagtg tcaccacatc cgcttcgagg
      661 ccgtcgatca ctgcgcgcgc ctgcttgccg gccccgccgt gggactgctt gatgttcacg
      721 cgctcgccgg tcttggcctt ccaatgcttg gcgaaggctt cgttgaattc ctggtacagc
      781 tctcgcgtcg ggtcgtagga gacgttgagt agcgacgtct gcgcgccgac cgactgcgtc
      841 atccctgcga acgcgaacag cgtcgcgact agccacttcc gtttcatgtt ttcctcttat
      901 tcagatcagg tcttgggagg cgtgaacata aagcgacaac gcttgtcgcg gaaatatttt
      961 cttcttgatt acttatgctt tgcaagcaaa attacgatgc gttatttgca tttcacgaca
     1021 cacaacgcat gcatttatcg ccaattctgc atattgcgac aacaacctag aggcccaaga
     1081 cgcaacgcct cagcgaagat cgttgagctt tgaacaggga agaggcgtac ctaccggcga
     1141 gcgccggaaa ggaatgcgcg cgaagacgaa gcgcaaacaa caagcggccc gttgcgggcc
     1201 gctttttcaa tcaaaactga tacgacttaa gacttacttc ttttccagcg aggtgtagcg
     1261 cagatagggt ttgaccgcgc gaaagccctg cggaaacttc tgcttcagca cttcgggatc
     1321 ggtgatcgag gggacgatga tcacctcctc gccctgcttc cagttcaccg gcgtcgccac
     1381 gctgtggctg tcggtcagtt gcagcgaatc gatgacgcgg agaatctcgt cgaagttgcg
     1441 gccggtgctg gccggatagg tcagcgtcag gcggaccttc ttctgcggat cgatgatgaa
     1501 cacggaacgc accgtcaccg tcgcgctggc gttcgggtga atcatgtcgt acagctccga
     1561 gaccttgcga tcggcatcgg ccaggatggg gaaattcacc acgcagcttt gcgtttcgtt
     1621 gatgtccttg acccagccgt ggtgggaatc aaccggatcg acggagaggg ccagcacttt
     1681 gacattgcgc ttggcgaact cctccgccag cttggcggta tagcccagct ccgtcgtgca
     1741 caccggcgtg aagtcggccg ggtgcgagaa cagcacgccc caactgtcgc cgagccattc
     1801 atgaaaacgg atcttgccga cggtggaatc ctgttcaaaa tcgggggcga tgtcgcccag
     1861 acgtagcgtc gccatggttt ttcctcgtgt aaggggttgt cggtacgaag actgcattgt
     1921 gatcatccac gcttattccg tgaacgaacg gtttgcaatt tctttataag cgagacccat
     1981 aaagacgcaa ggcgcgattc gttcagggaa tcgccgtcac cgacaaaggc gcctgccgcg
     2041 tgtgccagaa cacgtgaaag cgcccctcat gctccaggac catcggctgg ccggatgcgc
     2101 cgtcggtcgc cgccaggcga tgctcgcgcc aagtgcgacc gccatcgtcc gagaccatcg
     2161 cccggagctg cgtacgttgc ccgtcgaatt ccttccatgc cacggcaacc cggtcgccgg
     2221 ccgcggcgat gtcggcgtgc gcggcggcgt cgccgccgag gcgacgcaag ccgtcgaccg
     2281 ccccctcccc gagccggccg tagtacacgc cgctgtgccg atcgcctgcg gtaaaccaca
     2341 ccgcgtgcaa cgtgcccttg gcgtcgccga cgacggaggg gccgtgatgc gggcaggcgt
     2401 cgattcgcca gtcgtcgaag gtcacgcggc gcacaggacc ggccgcgccg ctcgcatcca
     2461 tgcgcgccag cgcgtgatcg cgcacgttcg gttcgaacac atggcgccac attgccagca
     2521 cgccgccgtc cgccgcagca tacaagccga tgcggcagca ttcgcaactg tgatcggcaa
     2581 gcttgtagtc gccgcgaaag ctcgcgccgc gatcgtctga cacggcgtag tacaccgccg
     2641 cgccgcgata agcgccgttg cctgcggcct tcgccgcgac cagatcgcgc ttgtcgatcc
     2701 acgcgacgaa gatccgtcct tcgcggtcga cggcgagcgc gtcgaaacga tgcgtgatct
     2761 cctgccggtc ggcgttgacg gtgacgggct cgctgaaagt ttcgccggca tccaccgaac
     2821 gcgcgaagcg cacgttgccc gtatagggtt tgctcaacgg ggtcgtccag gtgacataga
     2881 tttcgccctc gctgccgagc gcgattttcg gtcttgcatc gccgtcggcg gcgacctttt
     2941 ccggtttagg gttgaccggc tgcgcctcgg accaggtgcg cccggcgtcg gcgctgcggg
     3001 tcagcatgac gtggtcgccc tgtttgtaga ccgcccacaa gtcaccgctg cgggcatcga
     3061 aggcagcaga ggcgcccagg tcgatgcgca ccttggcggc gggcgcgtgt cgatgatcgc
     3121 cgtgcgcctg gacggaaagc gacagcgtca atccgagcag acccgcagcg atgcagcgca
     3181 ggaatttctt cagcatttcc ctctccttct caatgcgccc gctcatttcg cgtggcggtt
     3241 gaaccagtcg gtcaaccagg cgatatcgag cttgccgctg cgcccctgca cgacatgatt
     3301 cgcatcgtag aaatacgtgc gcggcaattc cccgtgccac ttccgatcga tggcgtagcg
     3361 cagacgctcc acatattcgt cggcaaaagc ccagcgttcg accgggccgg gatcgtagcg
     3421 ggagaggaaa cgccgcacat cgctccattc ctccggcaaa tctgtggaga ccagaacgat
     3481 cggcacctgc ggataccgtc gccggagatc gttccagtac gccatttcct cgcggcacgg
     3541 caggcaatgg atcgaccaca gcgccagcac gaagggcttg cccgcgtgct gctgcacgat
     3601 ggcatccaga ctgttcgtgt tgaagggacg cagttgatcc tgcgcccgca ctgcggtgca
     3661 ggcgtacagg acgagcgcgg ccgcaagcaa agcattgcgc agcaatcgtt tggctttcat
     3721 acatgcctca ccatttcatt tccacgccgg cgtaataggt ccgcggcaga cccggcgagt
     3781 aaaccggcgt gtcggaagaa atcgacgcgc tgtcggcata gcgcgtatcg cccaggttgt
     3841 agacgtgggc atacagcgcg acctgcttcg cgacctgcca gtgcgcgcgc aggttgtaca
     3901 ggttgtggcc ggaatacttt ttcgtattgg ccgcgtccat gtagtagctg cccaagcgaa
     3961 tccactccaa ttgcagccgg acggcttccg caggcgtcca ggtgagacgc gtattggccg
     4021 tgatgcgcgg cgcggcttcc atttcattgc cgctgaaatc gtcggtgccc acttcccagt
     4081 ccatgtattt gtgcttggca taggagaagg cgacgtcggc gcgccagtgt ctgcccagcg
     4141 gcgatcccag gcccagttcg acgccgcggt gccgcgtctt gccggcgttg acggtgcgcg
     4201 attcgttggt cgtggtatcc ctgaaagaaa ggatgtcgtt cttcttctcc aggttgtaga
     4261 gcgccaggtt gtacgacacg ccgctcagcg tcccgcgcag accgatctcg atctgctccg
     4321 tcttgatggc tttgagatcg agcgcggaat cgcgaaaggc ttcggcctgc gccgccgttg
     4381 ccgcactacc gggccggaac aactggttct ccgagggcgc gcggaaacca cggttatagg
     4441 agacgaagcc gtgggtctgc ggattgaatt cccaggtcgc acccagcttg ggcgtgagct
     4501 gattgaacgc cacgtcggtg tcgtccgcct ggccgtagaa gcgaccggcg acattgagca
     4561 cgccgccatc cgcgagccta ttgtcgaagt cgtagcgcag ccggtcatag cgcagcccgc
     4621 ccgtcagacg cagacgttcg atgggcgaga tctccgcgtg cacataaggc gagatgccgg
     4681 tgaacgagac gtcgtagtcg tacagcttcg gccctacggt gtagccggtg aagttgcgcg
     4741 atgcgcctgt gcccggaccg gtcgttgtca cgaaaatacg atcttcatcg cggctgcccg
     4801 ggctgtgatc gatatccacg ccgacgatga gccgcgcgtt catcgccccc gggaagtcgc
     4861 gccgccattt ggccatcagg ccgaaggact ggttctccgt cgtgtagacc gtcggatcgg
     4921 gcgccgcgag ccggaacgac gccagcagat ccatgctgtt gtgacggaaa tagggaatca
     4981 gcgacacgag atcgttgccg cgttcgtatt cgacggtcgt cgagaggcgg aaagcctcca
     5041 cgtcgcgaaa cgcgatggag tgattgttct tcttcgggtg gtgtttgtag tccgcacgcg
     5101 gcagcggcgc gttcgccccc gtgtcctgat cgattctcga atagctgagc agggttttga
     5161 ccagtgacgc gccggcgaaa tgatcccagc ggatggtggc gctttgacga tcgtaggcgg
     5221 tgtgatcgcg ccagccgtcg gtatgcgtga ggttgaggtc cgtccgccat ccgccgtcgg
     5281 catagcccgt tccgccgctg cccagcaggc gccaccagcc gtggctgccg ccctcggcgg
     5341 tgagcgacag ttcgggcgtc atcggcggtt cgcgggtcag tacgttgacg atgccgccga
     5401 tcgcatccga accgtacagc gccgtgcccg gcccgcggat cacttccaca ccgcccgcca
     5461 tgggcaggtt cgtttcgtac aaggcgttgt gattgaagaa accggtggaa cgcgtcggaa
     5521 tgccgtcttc gagaaacaga tagacgggat cggtggtgaa aggctggcga atggcggtgg
     5581 tgtgcccttc gccgttggtg accgacacgg cggccccggg cacctgactg atgatctgcg
     5641 ccggatgcgt gggcttcacc gcctccaccg cctcgttctt gatgatgccc accgaagccg
     5701 gcgttttcgc cagggcttcc ttttcccgcg taccggagac cacgacctcg ggcaacagct
     5761 cggtcgcttc ctgcgcatgg atttcgttca acgccgcatc ggcgaatatc gcgccgagca
     5821 gcagcgtacc gcccagcacc cgcgtgccga tggcgatgtg attcctgcaa accgcataca
     5881 tttgttttct tccctgtgtg cgcgcacctt gcgcgcaggc ttgaacatca gcgagcacgg
     5941 ccgggcggcg acagcgcgca gccggcggtc atgcatgcgt cgatcgatgt caggcgtgca
     6001 gaggcggcgc ccgggaaggc ggtatggcgt gaggcggtgc ggtgtgcggc gccggcacat
     6061 ccgttgcgcg cggcgcggat gaggcagccg caagaacggc caacggttcg cgcgcatcgt
     6121 gcagcgccgg atggaaatcg ccgaagacgc acagcgcgca atgcgtccgg tcctgatgga
     6181 cgccggtttc ggtcggcgtg ctgtcgcctg gcgcgatcca cttgaggccg gtcgaggtac
     6241 agaccgggat cagctcgccc gcgagcccat cgttcgccag cgccggggac cacggcaaca
     6301 gcagttgcca cagcatcgcc agcaaggcga tccagacagg cggacggcgg cggatggaga
     6361 ggacgctcac ggcgactcgg gcaatggaag cgcacgcatt ctatcctgcc tgcggcgaag
     6421 cgcgaaccgg ctcatgccgg ttcgcgcgtt tcccaacaac gccgcgcctt cccacttcgt
     6481 gcacgctcga gtgcccgtta atgtccgtgc ccgccggcag cctgcttcgc cgccggacgc
     6541 ggttcggtcg gatcgaatgc cttgaacgac gggttgttgt gcttgccgta acccttcttc
     6601 agcgattcgg tcatgtagaa atccggatcg tagggatgat ccttccacat atagaagtcg
     6661 gggtgctcga tgccttcgta ctcgaagacc agcatcgcgc cgccgccttc cttgccggcg
     6721 ccggtggtgt gctcttcgtt gtgatcgtgc gccatccaga tgcccgggtt gttcatttcc
     6781 acgatcatgt cgtagcgctc gccgggctgc atcggcagta cgtcgacgta gtacggcttc
     6841 ggcaacggca ggccgtcctt gtgcgtgacc agggagtcgt gaccgtgcag gtggaaggcc
     6901 accgggatgt tcggcgcgat caggcgcaga cgcatgatgt cgcccttctt cacgcgcacc
     6961 ggctggttat gcggatgcgc cttgccgttg atggagaaga aatcgaactc gcgcccgggc
     7021 aggccgttct cgccgtagtc cttcgccacg gcggaggccc agccggacag catcaggatg
     7081 acgtccttgg tgaccttctt ctccagcggc gtcggattct tcggatcgac gatcagcgga
     7141 ccccacatgc cgcgcaggtc gatgtgctcg gcggtgttga cgtgacagtg gtagaacagc
     7201 gtaccgggct tatcggcgac gaacttgtag gtgaagctgt cgccgggctc gacggccttc
     7261 tgcgagacgt ccggcacgcc gtccatctgc caggtcttcg tctggtagat gccgtgccag
     7321 tggatggtgt ggctcatggt ggtgttgttg gtcaacgtca cttccaccgt atcgccttcc
     7381 ttcacgtgga tcagcgggcc gggcacctgt ccgtcgtagg cccacacgct gacggtgaag
     7441 ccgggcgcca cttccagatc gagttcctcg atggtcattt cgaatttgcg cagctcggca
     7501 tgcgccgcga aggtgaagcc caggaacagg aggagggtaa ttgcaatgcg tttcgccatg
     7561 gtgtcggttc gcctttagtt tttctggcag cacagctttc gcagatgcgc tacgagtgcg
     7621 tggatgtcgt cgtcgttcag gttgccgccc caggccggca tcaggatcga tttgttgatc
     7681 gatttgccgc cgtgcttgat gaccttgaac agctcgtcat cggtgcgcgc cgacatttcc
     7741 ttggtgtcgg tgtgatcgcg cggctgcacc gacatgtgcg atgcgttgat gcctttgccg
     7801 ttggcgttca cgccgtgaca ctgcgtgcag taggtctgat aaagctgctc gccgtggtcg
     7861 gcctcggcgt atgccagacc gctggtggcc aggaacgcgg cggcgagaat gatgttcctc
     7921 atgactattt ctccccgatc gctttgagat agttcgccag cttgtgcacc gcgtcgtcgt
     7981 tcagatcgcc cttgggcatc atggtgtgcg gatcccagac gaccggatcg gcgatgtacg
     8041 cactgatgaa ccccggctgc agacgctgcc aggcggtgta cagctcgggc ccggagacgc
     8101 cacccttgcc cggtgcatcc tggtgacagg catcgcagcc cttgaacttg ccgaagttca
     8161 tcttgcccat gcgcaacgcg atgttgcccg gcgtgtagtt ctgcgcctgg atgagcttgg
     8221 tgaagggcgt cagcgtcatc agatagtcgg ccacggcttc cgcctgcgcg gccggcagcg
     8281 ccggatgctt ggtgaggccg tcggcggcga tggtgtcgcc gtccgcgccc gcctgcacat
     8341 gcgccggcgg gaacgtgccc gccgggcgga tgggctcggg tttttgcaac caactcacca
     8401 gccactcgcg gttgaacttg ttgcccgcgt aatcgagcgg cggcgccttg cgcgcggcgc
     8461 gctccgcaat gctttcggcc gcatagtcat gcgtcagcgc gtggcaactg ccgcattgct
     8521 gctgtacgtc tgccgccacg tcggcctgcg cggcgaccgg tgcgccggcg gcgagcagcg
     8581 cggcgacgcc ccaactgtat aagcgtttca cgtccactcc ttttctctca gattcgatca
     8641 gcgcaggatc agctgatcac cccgcccttt gttcggatcc gcatccttgt gattcatcag
     8701 aatgctgatc ccgccgtgat gcgctttctt catcgcatgg tcgaccagtg cgttgttggt
     8761 cggccggtcg gcagggctga cgaggtccag ggtcacggcg tgtgcgggcg gcacctgcat
     8821 ggtctgcatg ccgtagagca cgttcttcgg attaccgttg tcccatacgc gatcccagat
     8881 gccggcgatg ggatggaacg ccgcccactc gttgatcatc gcgttgacga agtagatccg
     8941 tacgcgctcg ccgggcttgg attccagcgt gatgctggcg ttggagtcgt gcaccgggtc
     9001 gtaatggaag accttgccgt tcaccagcac gcccttccac ttgaggttgt cggtgcgctc
     9061 ctcggcggag gcgcccgctt cgaacagatc gccgtgcacc agcacgtatt cgcgatcggg
     9121 cttgggatag gcgtcgctgt agccatcctt cggatcgacg atcaccacgc cgtacatgcc
     9181 gcgcgaaata tgctcggaca tggaatcggc gccgcagtga taaatccata cgccggggtg
     9241 ctcggccttc caggtgaact gctttttctc gccggggttg atggtttcga actcgccgag
     9301 cacgtcgaag cgtccggcgt ggaagtccat ggagtgactg ttcttgttct tcggatcgtt
     9361 gatcagcgtg aagtcgatgg tgtcgccttc cttcacgcgc accagcggac cgggaatggt
     9421 gccgttgtag gtccacatgg gttgcttgtt gccggcgtta tcgatgacca agtccacttc
     9481 gcggaccgga atctccactt tcacggtagc ggcaatggct tcggtgccga gcagcgccgc
     9541 gctgagcagc agcgcggaca ggagcggttt ctggttcatg cttttgtgtt cctcatggtt
     9601 tttggtgtag gctcgctttt gcctatatca aaaactggac cagaaaattt tttgctttca
     9661 attcatttgc ttgccatttc tttaatgtaa gttaaacaac agcgacaccg atgttgtttt
     9721 gacaactgcg atgtagccat gaccacgaac gcctttcata acctcgccgc cgccgtagcg
     9781 gatctctcca aggccatgac accggaggtg cgctacacgc gtctgctgga tgcgttccgc
     9841 aacagcttcc cctgcgatgc gatcgcgctg ctcgaacgcg acggcgaagt gctgatcccg
     9901 cgcgccgtcg caggtctgag ccgcgatacg ctggggcggc gcttcgtcat tcacgatcag
     9961 ccacgcctcg cgcaaatcct gcacagcgcc gatccgatcc gcttccccgc cgactctgcg
    10021 ctgcccgatc cctacgacgg cctggtggaa cacgagaccg gcgactcact ctacgtgcac
    10081 gactgcatgg gcgcatcgct ctatgtggac ggtcagccgc tgggcgtggt tacgctcgat
    10141 gcgctgcggc cgggacactt cgacgaaatc gattacggcg tcttccaggc cttcgttgcg
    10201 gtggcggccg ccaccgtgcg cgcagcgggc tggattcaac gcctggaggc gcagctcgaa
    10261 cgccatcacc tcatccagcg cctgaacgtg caggacggca gcggcgaaga gttgatcggt
    10321 cagagcctgg tcatgcagca gttgcagcac gaagcggaga cggtcgcgcc atccgacctg
    10381 ccggtgctga tcctcggcga aacgggcgtg ggcaaggaat tggtcgcgcg cctcatccac
    10441 cgccactcgt cgcgcgcgcg agaaccgatg gtctacatca actgcgccgc cctgccggag
    10501 tcgatcgccg aaagcgagtt gttcggtcac gtgaaaggcg cgttctccgg cgcctccgac
    10561 gaccgcatgg gcaagttcga actcgcgcac gagggcaccc tgtttctcga cgaagtcggc
    10621 gaactgccgc tgcccgtgca ggcgaagctg ctgcgcgcat tgcagaacgg cgagatccag
    10681 cgcgtcggct ccgacgcgca ccatcgcgtc gacgtgcgcg tcatcgccgc gaccaaccgc
    10741 gatctgaaga aggaagtgac ggacggacgc tttcgcgccg atctctatca ccgcttgagc
    10801 gtttatccgc tactggtgcc gccgctgcgc gaacggggcg aagacgtgct gctgctcgcc
    10861 ggctatttca tcgaacgcaa ccagcgccgc ctgggcctgc gcgccgtgcg gctcggccgc
    10921 ggtgcgcgcc aatggctgac gcagtacaac tggcccggca atgtgcgcga actcgaccac
    10981 accctgtcgc gcgccatgat ccgcgccttg agcgtcgcct cgcgccgcga tcgcgtcatc
    11041 gaactcacga cccagcacat cggcgccgac ggcgcacgca tgccgcattc ggacggcgcg
    11101 cacatcgccg aagcggtgaa cgatcaacgg ccgttgagcg aagtgctgga tcagacgcgg
    11161 cgggaaatca ttcaggcgcg tctgcgtgcc catcacggca accgtgcagc ggcggcgcgc
    11221 tccctgggcc tggaccgcgg caacttccat cggctgctga aacggatggg gttgaattga
    11281 attagccgag agccgagagt cgatggttcg actcggctct gcggaccggc tcaccttgag
    11341 cggaaggcca aaaagccgga ttccggctcg cgccctgcgg gcttggccgg aatgacggcc
    11401 catccatcgt cattccgggc gagtggcgca gccacgagac ccggaatccg gcgcagcggg
    11461 tgaaacctaa agcctcaaac acggagagaa ttcaagcaag gtccgctctg cgtgccctct
    11521 gcggttcaag gccccgactc tcgctacgcc gcccgcagcg catccacgat gcgcagccgc
    11581 gcggcgcgca gcgccggcaa cacgccgccg gcgaagccca tcaacaacgc gaacaccagc
    11641 gagcgccata cgatgtccgg tgtcagcgtg aagctgaacg cgagttcggc gaaagactgc
    11701 cagttcatgg tcgagatgtg caccagttgc atgagcgatg cgagtgccac accgacgacc
    11761 cagcccaccg cactcaacat caacgactcg agcaggaagg cacccaggat gccgctgcgg
    11821 ctgaagccca gcgcgcgcag tgtgccgatc tccgccgtgc gattggcaac cgcggcatac
    11881 atggtgatca tggcgccgat catcgcgccg atggagaaga tgatcgacag cgtgatgccc
    11941 agatagctga tgaacgtgga catggtcttc gactgctcct cgtagaacac gcgctcgcgc
    12001 ttcacgtcca ccggcaggcg cggatcgttt tcgatcgccg ccttgaagtc gtcgaaggcc
    12061 tccatgctgc gcaaccgtgc taccaccgac gaataggcct ggcgccggaa cgcctgcatc
    12121 agttgctcgc tgtcgcccca cacttccgaa tcgaaggccg ccccgcccgc ttcgaacacg
    12181 ccgaccaccg gccactcgcg cccgccgaag cgcagccgct gtcccagccc cgcgccttcg
    12241 aaacgctcgg ccacgccgcg ccccaccatg atctcggccg tgccgaaacg cagcatgcgg
    12301 ccggacacga tttccacctg cggacgcacc gcgagcccgg ccggcccgat gccgcgcgtg
    12361 gtgatgttgg tctgcacgcc cgtgtcgcgt ttgggcaggg tgatcagcac caccgtctcc
    12421 ttcgaagcca gcggccggcc gtccgcaccg atcgccaccg ccgcattgct ctcgataatc
    12481 gccgcctgat tgcgttcgat cgaactctgc acttcggtgg cggcggcccg gcgcgtgacg
    12541 agcacgttgt ccggctgccc ggtcgcaacc agtgccgcgc gcaatccggc atcgagcatc
    12601 agcaccgcgg caaagacgaa taccaccagc gccataccgc ccgcggtcag cagcgtcgtg
    12661 agcttgcgcg cccacaggtt tcgcagcgaa taggaaacgg gaatgcgcat cttcaactcg
    12721 cgcgctcagc cgacaaagcg taagccctcc acgatcttca cgcccgcggc acggcgcatg
    12781 ggcaagagcg ccgcaaccac accgacgatg agcgcgcacg aagcctgcag caaaatcgtc
    12841 tcgcgactca ccccgaatac gggaaacagc gtccccattt gcgaagcgaa ccagtgcccg
    12901 atgggaaagg tcagcccgat acccagtgcc gcgccggcca cggcgatggc cagcgattcg
    12961 ccgagaatca gaccgccgac gaaagccggc tgaaagccca gcgccttcag cgtcgcatac
    13021 tccgccatgc gctcgcgcgc ggtcatcgac atagtgttcg ccatgacggc caggatgatg
    13081 gcgatcacca cataggacac gaagcgcacg gcgatcacga tcgcctccgt ctgcttggcg
    13141 aagccgatct gaaacgcctt ctccgtttcg gtcagcgtct cggcggcgga attgcggaac
    13201 aactcgtcga tttcgcggcc gatgtccgcc gcgcgatcga cgtcgtcgat ggacacgacg
    13261 aacacgccga ccttgtcggc gcgtgccggc gcacgcttct tcaacgactc gttgaggtac
    13321 tcgtagtgga agaacatcag cgaggtgtcg gtgctcggct gcgcgccgtc gtaaatggcg
    13381 cgcacggtga actcccagtt gcccgggaaa atgtcgccct ggatgggaat cacgtcgccg
    13441 atcttgaagc cgtggttctc ggcgatcttg cggccgatga tcgcgccctt gcgatcgcgc
    13501 aggaaggtgc ggaactcgtc ctcgtccagc cggtattccg gatacatggc gaaataggga
    13561 acgggatcga cggcgaagcg cggaaagaaa ttcgccatgc tggtgtcgcg atagacgccg
    13621 ccgaaccagt tcgccggcgc cacgccggtc acgccgtcga cggcgcgtat ccgctggccg
    13681 taatggatgg gcatgggaaa gaccagcgag acggcgttgc gcgtcaccag gcggttcgcc
    13741 gccgccgcgt cggcgcccgc gtaccacgca tcgacaatgg tctgcagcag gccgaaggaa
    13801 aggatcgcca ccaccagccc gagcacagtg agcccgctgc gcagcttgtg acgcagaccg
    13861 ttgcgcagga tgagcttgag gaaatgcatc ggcgcctcag tggtggcgcg atgcgcgcac
    13921 cgagcgttcg gcgatgtccg ccgaagtgga gagctcgccc ttttccagat acatgatcaa
    13981 gtgcgcctga tcggcggcgc gcctgtcgtg cgtcaccatg atgatggtct tgcccagttc
    14041 ggtgttgagc cggtcgagca atgcgaggat gtcctcggcc gaagcgcggt ccagatcgcc
    14101 cgtgggttcg tcggcgacga gcagcgtcgg atcggtgatg atggcgcgcg caatggccac
    14161 gcgctgctgc tggccgccgg agagttcgga aggcaggtgc gccatgcgat cggccagccc
    14221 caccagttcc agcgccattt ccgcgcgctc gcgccgttcg cgtcgcgaca ggcgggtgag
    14281 cgtcagcggc aactccacgt tctcgaacgc gttgagcacc ggcatcaggt tgtagaactg
    14341 gaagatgaaa cccacatgtg ccgcgcgcca gtgcgccagt tccgcttcgc tcaaccgggc
    14401 gatgtccacg ccctcgatca gcacttcgcc cgagtccggc ttgtcgatgc cggcgatcat
    14461 gttcagcagt gtgctcttgc ccgagcccga agggcccatc agaccgagaa actcgccgcg
    14521 acggatatcg atattgatat cggtgagcac cggcacgcgc tgaccgcccc gactgtagct
    14581 tttgacgaga tggcggatgc ggacgagcgt gtccgggtcg gcggcgttca cggctgctgc
    14641 gccaccgtca ctttgcggcc ggcaatggcc ggatcgagcg gctgcgcgac caggcgttgc
    14701 cccggctcga cgccgctcac ctgcaccaga tcgccgagct tgccgcccgt ctgcacggcg
    14761 acgcgttcgg cgcgcccgtc gcgtatgcgc agcacggccg tgccctgttc atcctgcagg
    14821 acggcgccgg ccggcacggc gatcacaggc gcgcgttcgt ccgcgcgcaa gggccgctcc
    14881 agaaacgcca ccttggcgct catgtccggc agcacgcgct gatcgcgttc gtcgaaacgg
    14941 attttcacca gcaccgtggc tttagtgcga tccaccgtcg gcacgatgcg attgaccgtg
    15001 ccgccgaagc gttcgtccgg gatggcgtcg agttgaatga tgaccggctg ggcgacctcg
    15061 atctggccga gcgaagattc cgccacatcg gcctccactt ccagcgtgct catgtcggcc
    15121 atggtcacga ccgcgccctt ggtgtcgagc gcgctggaga agggcgtgat gttgtcgccc
    15181 acattggcgt tcttggtcag caccacgccg tcgaaaggcg cgcggatcag cgtctggtcg
    15241 aagccgacct gcgccgcttc gagattcgcg cgcgccaccg cgagcgccgc ctcgtgactg
    15301 gccagcgccg cctgcgccac ttccagacgc gcacgcgcct gatcgtgcgc cgcttcgctg
    15361 atgtagcgct tgccgatgag gtcttccgag cgcgcaaagg cgcgcttcgc ctcacgcagt
    15421 tccgcgcgcg cctgttcgag attcgcctgc gccaccttca cgttcgcctc ggcctgctcg
    15481 cgcgtggcag cgacatcgcg attctccagc cgcgcgatga tttcgccttc cttgactgtg
    15541 ctgccctcgg agacgcccag ccattccagc cgtccggtcg ccttcgacgc gatggaggcc
    15601 ttgcgctgcg ccaccacata gccggtcgcg ttgagcaggg tgatggcctg tgcgggatag
    15661 gccgtcgtca cgctgacggt ctgcacttcg accgcaccgg cgtagcgctg ccagaggatc
    15721 gcggccgcga ccagcacggc gacggcgatg ccgagccaca gaccccagcg cacacgccgg
    15781 gggccgccgg gacgcgcagc gcgacggtcg atgctgagac gggaaagttc ggaagcgctc
    15841 acggaagggg cgatggacac gaagcgcgaa ttatagccag cggcgtgccg ctgccccgga
    15901 catcggcgcc tttgactttt gccgctcgcg tccggatgat cgggccgtca tccgcatcgt
    15961 caccagccgc ctcatgcaag ccttcatgcg cccgctcgtc tgcgccctgc tgctcggcat
    16021 cgcgcttccc gccgtcgccg aaacgccgga tcccgtgctg ttcgcggtgc gggtggaagc
    16081 gggcgacctg cccgcggccg aacaatggct cgcagccggt ctcgatccgg actacgaggg
    16141 cgatcgcatc ggcagcggcc tgatgatcgc ggcatgggaa ggcaatgtgc cgatgatggc
    16201 gctgtttcac cgttatggtg cggatgtgaa ccgcatcaac cgcaacggcg agcaggcgct
    16261 gctgcacgcg gcatggaaag gacgcaaaag cgcggtcgaa tggctgctcg ctcacggcgc
    16321 gcaactggat cgcccggggc gccactggtc ggcgctgcat tacgccgcct tcgccggcca
    16381 cgaggacatc gtacgtctgc tgatcgagcg cggtgccgac gtcaacgcgc tggcgccgaa
    16441 cggttccacc gtgctgatga tggcggcgcg cgaaggccat ggtgcgctcg cgcaattgct
    16501 gctcgcacac ggtgcgaacc ccgcggcgcg caacgattac cgcgaggacg cggtggactg
    16561 ggcgctgcgc tatggccata cgcagatcgc ccggacgctc agttccgccg aacgggtcga
    16621 acgcgcggtc caggttgccg cgctcgcgcc gccggtcgtc gaatccgtac ccgcgccgcg
    16681 cccgctcgcc gagttggtgg aagctctgcg ggaagcccgt gccgcaggac gccccaccga
    16741 tgaagtgttg caacgctacg ccgtcaccct cgacgcgctg aagcactgac ctcctgcatc
    16801 gatccccggc cctgcgcttc ccgttccatc cgaacgatcc gcaaactttt ggtcgcaagc
    16861 agtcggcatg cctgaaccgg ccgctgtatt tgtctgtaac aaacgggtcc cggtcttgct
    16921 ttactgagcc gctgcaacgc gttgcgcgtc cgcaacagcc tcccgaaaat cggagagctc
    16981 gcgaagcgct tcctttacta ttttcgaccc tgctcgagca gaccagtgat gcctgcgttt
    17041 cgcatccggt ggaatttcgc cgtcaaagcc gtggtgtccg ccaccaccgg gctgctattc
    17101 gcctcggtct tcgcgcacac cacactctac gaacgactcg acggctggct gcaggactcg
    17161 ctgcagcgca ccgtgtccgc tcccatcgac ctcagcgaag tgctggtcgt cgatatcgac
    17221 gatgcctcgc tcagcacgtt gctgcgcgcg ctcggtccgt ggccttataa ccgcgacacc
    17281 ttcgcctaca tccagtcgta cctgatgcaa cagggtgcgc ggctggtcgt gtacgacctc
    17341 ctgctaaccg atgcgcgcga tgccgcgccg aatcccctcg atgtgcccca gaacatcgtc
    17401 tatgcgggtg ccggtttcgc cgtgccgctg cccgtcacgc cggcctatcg cacgcaactg
    17461 gagacgctcg cgatcggccg cgacgaccgt ctggtggatg aaagcggcgt aaacacgggc
    17521 aacggctatc gcagctggtc ctatctgaag ctgccgctcg tcgacgagca gacgcgtcac
    17581 gcggtgggcg tgatgaacat ccagcccgac gaagacggcg tcgtgcgccg cgtcgcgctt
    17641 ttccatggcg cgcagggcta cgtcctgccg agtctcagcc tggccgcttt gatggcagcg
    17701 catcccgaac aggtcgccgc cgactggccc aatcgcaggt tgcgcgtccg gcgcaccgat
    17761 ctgccgctta ccgagcacgg cgaagtgctg ctgcgctatc cgtcgaatac gaacgagctg
    17821 cgtgtcgtgc ctttctacga gctgatgttc gccgcgacgg agccggacga cgccatgcgt
    17881 tcgacgcgac agttgaagga cagagtggtg ttcatcggca gttccagttt cgccggcggc
    17941 acgcatgcct atacgcccat ggaccgcatg cccggcgtgc aactctccgc gctgacttac
    18001 gccatgctgg cgaacggcag cgtgctgtcg ccgccgaagc tggcggtgga cggcgcgatc
    18061 gccgcgttcg cactgtcgct gccgctgctg ctgctatggc ggggcgtcga gtcgagcggg
    18121 cgcgccttct tcatggtgtt catcggtctg ccattgttgt gtgtgggcat cgccatcgca
    18181 ctgttgatgg gcgggcagtt gtcgaactgg ctgtacgcgg tcgcggcggc gctggccgca
    18241 tgggcgacaa tctttgcgat ctggctgttc gacatcgcca acgatcggcg tcgtctgcga
    18301 tacgaagcca tcgccgcgcg cgaagcgaat cgcctgaaga gccagttcct caatcaggtg
    18361 acgcacgaac tgcgcacgcc gctgaccgcc atcatgggtt tcaacaagat caaccagtac
    18421 accgaagacc tgggacgcga agcgcgcatc aagaacagcg aaatcatcgg ccgcaattgc
    18481 gatcacctgc tcgcgctcat caacaacaat ctcgatcttg cgaaaatcga agccggtgcg
    18541 ctgagcattg cgctcgcccc cgaggacccc gaacagctat gccgcgacgt cgtcgcgacc
    18601 ttgcagcccg tcgccgacga gaagcgcctg cgtctaaagt tcacgcgtgg gacggaattg
    18661 cccgccgcac tcatgctcga tgcgtttcgc gtgcggcagg tgctgatgaa cctgatgggc
    18721 aacgccctca agttcacgca gaacggcacg gtcgaactca ctgcgctata ccacgcaccc
    18781 acgctcgtgc tcgaggtgcg cgatacgggt gccggcattc ccgccgatgc gctggagcgc
    18841 attttccatc cctatgagca ggccgactcg tcggtggcgc agcgcttcgg cggtacgggt
    18901 ctcggcctgg ccatcacccg caccctgatc gaactgatgg acggcgtgat cgaggtggag
    18961 tccgaacccg gcgtaggctc taccttccgt gtgcgcctgc ccgcggaagt gactgtccgc
    19021 cccgacacgg tgcgtcccat taccgacgcg cggcccatgg aaattttgtc cggccgtgta
    19081 ttaattgccg aggataacga agacatccgc gcgctcattg cgctgcaatt ggggaagctg
    19141 ggtgtcgaga ccgtaacagt gccgcatggc ctcgccgcgg tagaaacggc gctggccgac
    19201 agcttcgatg ctgtactcat cgacatggaa atgccggtga tgaacggctt cgaagcggcc
    19261 aatgtgctgc gcacccgcaa ttaccaaggc acgatccttg ccttgaccgc ccaccaggag
    19321 gggatggaaa tcgaacgcgc actcgcttcg ggttgcgacg gtgtggttcg aaagccagcc
    19381 agtatcgaat ctttgcgcgc cgccttgcgg ccggtcctga ggggatcgcg ccgcgcaacg
    19441 ggcagcgcac aggccggtta gggaggtgtc accatgcaag gcttaccaac gcatacgccg
    19501 gatcgtgcgc gcggttttgg tacatcgcgc agcacggcac cttcgcgccg gatttgcgtc
    19561 cgggtcgcca gcgaactgga gccgctcatt ccgcgcttcc tcgccaaccg ccgtacggaa
    19621 atcgcctcgc tcgaacaggc ggtcgaatcg cgcgatgccg aaacgctgcg tcgcattgcc
    19681 catggcttga agggtgcggg cggcggctac ggcttcgacg agatcacgcg catcggcgcg
    19741 caactggaag gctgcgccaa ggcgggcgac gaccagggcg cgcgaccgct catccacgcg
    19801 ctcaaacgct atctcgacga cgtcgaagtc cgtttcgact gaacacccaa acaaggggat
    19861 catcatgcgc atcgcgctgc tcgaagacga ctccgcccaa cgtgatctgg tcaagctgtg
    19921 gctcgacggc gccgggcata tctgccacgc atacggccgc tcgaaggact tcatgcgcat
    19981 cctcacgcgc gacaccttcg acctgttgct gatcgactgg gaactgccgg acgtgaacgg
    20041 cgatcgcgtg ctcgaatggg tgcgcggcaa tatgcgcgaa tacgtgccgg tgatgttcgc
    20101 caccgcgcgc agcgaagagg acgacatttg cgccatcctg cgcgcgggcg ccgacgacta
    20161 cctcgtcaag ccgctgcgca agaacgaact gctcgcacgc atcgaagcgc tgggccggcg
    20221 cggcaagccg ccggcgcatc agaacgaacc cttgcgcgtg ggcgatttcg agatcgactt
    20281 cgaatgccgc gtgctgcgtc gcggcggcgt gcccgtgctg ctcacgcaga aggacttcga
    20341 tctggccgtg ttcctgttcc gcaacgtcgg cacgctgctg tcgcgcggcc acattctcga
    20401 aaccgtgtgg ggccgcaatc ccgacctcaa cacccgcacg gtcgatacgc acatcagccg
    20461 cctgcgctcc aagctcggcc tgacgccgga aaacggctgg cgcctctcgg ccgtatacca
    20521 gcagggctac cgcctcgagc ctgtggccga gcgcgacgcg ccggtattcg cgcgacgcaa
    20581 gccgtccgcg ccgctgcaga tcgccacctc tccctgagcg ccccgaccga catccgcccg
    20641 cgtggcggat gctcgtttca ggccacagcg gttcggcacc ccccgcctct ttcccgtttg
    20701 cttcgggcgg tcgatcttag cgatgattca tgagatgctt tagcggctca tcgccatcga
    20761 tcgatcgccc cgcgcgaacc gcatcgatgc gatgatgcac tcacgcatcc gcccttccgg
    20821 cgcgcccggc gccgcaggcc gacgacactc ggccgaacat agggcagacg cattgcaacg
    20881 tagaaaactc cttcgccaga cctgctgagg atcggctatc ggccggctag gtgctatgaa
    20941 ttttcttcct tacccgcgcc ttggcgcgtc gtcttctccc tgggacaaca actcgccggt
    21001 gcgcgaggag ctgttcggca tcgaacggct cgaacagcat gcggaaagcc tcgccgcggc
    21061 acaaacgatc actaaccgcc cgcccgcggt gctgacgctg cgcgcccgtc tgaagagcaa
    21121 cgccaaggcg ctgcgcgcgg cctatcgcat ctacgcgtcg gaactggagc gcggccatac
    21181 cgtcgtgccc gccgccgact ggctgctcga caacttccac atcgttgagg agcagatccg
    21241 cgaaatccgc gacgacctgc cgccgggcta ctaccgccaa ctgcccaaac tcgcgaccgg
    21301 ccccttcgcg ggctatcccc gggtcttcgg actggcgtgg gccttcgtgg cgcataccga
    21361 cagtcatctc gataccgaca tcctgcgccg ctacctggcg gcctatcagc gcgtgcagcc
    21421 gctcaccatc ggcgagttgt gggccgttgc gatcacgctg cgcatcgtct tgatcgaaaa
    21481 cctgcgccgg cttgccgacg acatcaccgc cgtgcttgcc gcacgcgccg ccgcgaacgc
    21541 gctggcgaac cggctgatcg aagcggacga cgtccgcgcc gtgctggaaa acgacggtgc
    21601 ctatcgttcg tccgcactct tgtccgcagc cttcgccgcg cacctggcca aacgcctgcg
    21661 cgagcaggac cccaagacga tgacggcgct cgagtggctc gaggaaaggc tcggcgggca
    21721 ggggacgacc atcgagcagg tcgtgctgca ttcgcagcaa cagcagagcg cctccaacct
    21781 cagcgtgcgc aacgtcatca ccagcatgcg actcatctcg aacgtcgact ggacggaact
    21841 gttcgagagc gtcagcctgg tcgatcagcg gctgcgcgcc ggcagcgcct tcggcgccat
    21901 ggatttccgc agccgcgatc tctatcgcga tgccatcgag gacctcgcac gcggctcgtc
    21961 gcgcaccgaa ctcgaagtcg ccgacctcgc gatcggggcc gcgcaagctg cagcgtccga
    22021 agcgcccgat gaagcgcagg cgctacgcgt aggcgatccg ggctatcacc tcatcgccgg
    22081 tggccgccgc gcattcgaac aggccatcgg cttcaagtcc cccttccgcc tgcgcctcaa
    22141 ccgcctcaat ctccgcctcg ggattcgcgg ttacctcggc gccatcctgc tcaccacgac
    22201 gcttctgctc gcactggtgc tgcaggcgct cgcgctgccg gacctggacc gcgcatggct
    22261 ggtgctgttc gccctggtcg ccttcctccc ggcgacggag gtcgccacgg cgctggtcaa
    22321 ccgcgcggtc acgcgcggct tcggcccgcg catcgtgccc ggcctggaac tcaagagcgg
    22381 cgtgcccgaa tcgctccgca cgctggtcgc cgtgccgacg ctgctggtca atgaggacga
    22441 cctgctggaa cagatcgagc ggctggaaac gcacttcctc tccggcatcg gcggcgacct
    22501 gacgttcgcg ttgttgaccg acggcgcgga cgccgacagc gaggtcgtgg agagcgatgc
    22561 gcgactgctt gccctggcca ccgaagcgat cgcggaactg aacgcgcgtt acggcccggg
    22621 acccgccggc gatcgcttcc tgctgctgca ccggcgccgg gtgttcaatc ccgccgaagg
    22681 caagtggatg ggctgggagc gtaagcgcgg caagttgcac gaactcaacc ggctgctgcg
    22741 cggcgcgacc gacacgacct tccaggccgt cgacggacga ccaccgcagg tgccggcgaa
    22801 cgtgcgctac gtcatcacgc tcgacgccga tacgcgcctg ccgcgcgatg cggcggcccg
    22861 cctggtcggc aagatggctc atccgctcaa ccggccggtc ttcgacaaga cggcgcaacg
    22921 cgtggtcgac ggctacgcca ttctccagcc gcgcgtcacg cccgcattgc cgctgcgccg
    22981 cgagggcacg ctctatcaga aggtgttctc cggtccgggc ggattggacc cttatgccgc
    23041 cgccgtctcc gacgtgtatc aggatctgtt cggcgaaggc tcctacaccg gcaagggcat
    23101 ctacgacgtg gacgccttcg aagcagcgct ggccgggcgc gttcccgaga acacgctgct
    23161 cagccacgac ctgttcgagg gaatctacgc gcgcgccggt ctggtgacgg acatcgaggt
    23221 gttcgaggat ttccccgcac gctacgacgt tgccgtgaag cgccagcacc gttggacgcg
    23281 gggcgactgg caactgctgc cgtggatctt cggcttgcgc cgcggtccgg gtggattgcc
    23341 ctcggtcggc cgcggcaaga tgctggacaa cctgcgccgc tcgctgctgc cgccgttcac
    23401 cctgctgaca ctcggcgtgt gctggctgct gccgttgccc gccagcttgc tgggcacagc
    23461 gatcgtgctg gcggccattg cgatcccgcc gttcctgccc gtgttgttcg gcgtgatgcc
    23521 gccccggggg atccgtctgc gcaaccactt gcgcgcgctc gccggggatc tgcggctcgc
    23581 cgccatgcaa agctttttcg cactcgcgtt tctccccgat caggcctggc gaatgtgcga
    23641 tgctatcctc cgcacgctgg tgcgcctggg catcacgcgc cgccacctgc tcgaatggac
    23701 gacggcggcg caagtctcca gccgccccac gcccggcgtc gaaggcttct atcgcaaaat
    23761 ggcgcccggc accgcactcg gcctgctcat cgcggcaggc gcagtgctgt gcatgccctc
    23821 ctcctggccg gtcgcactac ccgtcgtgct gctctggctg agcgcgcctg ccgttgccta
    23881 cagcgcgagc cgcacgcctc cggcggcgca tcaggccatc gccgcaccga ccgcccaggc
    23941 gctgcgcctc gttgcgcgac gcacttggcg cttcttcgag accttcgtga caccggccga
    24001 ccacatgctg ccgccggaca acttccagga ggaccccgcg ccggtcgtcg cccgccgcac
    24061 atcccccacc aacatggggc tttatctgct ctccgccgtc gccgcgcgcg acttcggctg
    24121 ggccggtacc gtggagacgg tcgagcggct ggaggcgacg ctcgacacca tggtcaaact
    24181 gccccgctac aacggccact tctacaactg gtacgcgacg gcggatctgc ggccgctgga
    24241 gcccgcgtac gtgtcctcgg tggacagcgg caacctcgcc ggccacctca tcacgctcgc
    24301 caatgcctgc gaatcgtgga tcggggaacc gcttgcaccg aacgcccgcc tcggcgtgct
    24361 cgatacgctg caactggtgc gcgaaacgct cggcgcaaaa ctgaatccgg acgaccggca
    24421 cggacggcaa ctcgctgccc tgctcgacga gttcgagacg caattgaacg gcgcgcaatg
    24481 gatcgaaacc ctgccgtcgt tgaaacgcta tatcgacaag gccggaagaa ccgcgctgga
    24541 ggtcgttccc gccggcgaag gcgatcgcgt gcccgaagcg gtgtactgga tcgaagcact
    24601 gcgcaagacg ctggccgagc atgcccgcga ccggctgcaa cgcgaggaca cgcaggcatt
    24661 ggacgaccgc ctgcgatcca tcgccgcacg cgcgcgggaa atggcgcatg ccatggactt
    24721 ctccttcctg ctcgaccgcg aacgcaagct gctgtcgatc ggctactcgc tggccgacaa
    24781 ccggctggat caaagctgct acgacctgct cgcatccgag gcgcgcctgg ccagcctggt
    24841 cgcgatcgcc aagggcgatg tgccgacaca gcactggttc cggctcggcc gcacggccac
    24901 gccggtgcgc aacggctcgg cgctgatctc ctggtccggc tcgatgttcg aatacctgat
    24961 gccttcgctg gtcatgcgcg cgcccgccga cagcctgctg gagcagacca accggctggc
    25021 ggtgcggcag cagcaggact acggcgcatc gctcaacatt ccgtggggca tgtccgaatc
    25081 cgccttcaac gcccgcgacc tggagtacac ctatcagtac tcgaacttcg gcgtacccgc
    25141 cctgggcctg aaacgcggcc tgtccgagaa catcgtcgtc gcgccctacg ccaccggact
    25201 tgccgccatg atcgatccgt acgggtcggt gcagaactac aaccgcatcg cggcgttggg
    25261 cggcctgggc cgctacggct attacgaggc gctcgacttc acccgctcgc gtctgcacga
    25321 aaacgagaac gtcgccatcg tccgcaccta tatggcgcat caccagggta tgccgatcgt
    25381 cgcgatcgcc aacgtcatcc acgcgggcga aatgcgcgca cgcttccatc gcgaaccgat
    25441 gatccaggcc agcgaactgc tgctgcagga acgcatgccg cgggtcgcgc cgatcatgcc
    25501 gccgcgcgcg gaagagatca gcgtcgcggc ctgggccgaa cagaacgaag gcacggcggc
    25561 gcgtcggctg gagcctgcgc ccgacgacgc gcccgtcact catctgctct ccaacggccg
    25621 ctatgcggtc atgctgaccg catccggttc cggttacagc cgctggcgcg acatcgccgt
    25681 tacccgctgg cgcgaagacg cgacgcgcga cgactgggga tcgttcatct tcctgcgcga
    25741 tgcggacagc ggaaagcgct ggtcggtggg cgcgcagccg ctcggctggg gcgacggcca
    25801 gcggtcggtg gtgttcgacg aggatcacgc cgaattcaat tatcgggagg gcgccctcac
    25861 cacaaccatg aatgtcgtgg tctcgggcga ggacgacggc gaagtccgcc gcctgtcgct
    25921 ggtcaacagc ggacgccagc cgcgcgtaat cgaagtgacc tcctatgcgg agatcgtgct
    25981 gaccacaccg gccgcggacg aggcgcaccc ggccttcgcc aagctgttcg tgcagaccga
    26041 attcctggag gaattcggcg ccatcgtcgc cacccgccgg ccgcgcgcac agaacgaacc
    26101 gcccatctgg gccgcgcact tcgcagtcgt cgaaggcgag gcggccgccg cgccgcagta
    26161 cgaatcggat cgcgcgcgtt tcatcgggcg cagccggacg atcgccacgg caactgccat
    26221 cgtcgatgcg cggccgctgt cgaacaccgt cgggacagtg ctcgacccgg tgttcgcgct
    26281 acggcaccgc gtcaccatcc cgccgggcgg cgaggcgcac atcgccttct ggaccgtggt
    26341 cgcaccgacg cgggaaacgc tgatcgatct gatcgacaag aaccatgatc gccacgccta
    26401 cgaacgcgcc agaacgctgg cgtggacgca ggcgcagatc cagttgcgtc acctgggcgt
    26461 caaaccggac gaggcggcgg atttccagcg cttcgccgca ccgctcattt acgcggactc
    26521 gcgcttccgc gcgatgcccg agaccctcct gcgcggcgcc gacgcgcagt cggcgctgtg
    26581 gccgcatgcg atctcgggcg acctgcccat cgtgctgctg cacatcgacg aaatggagga
    26641 catggcgcag gtccgccagc ttttgcgtgc gcacgaatac tggcgcatga aacgcctggg
    26701 cgtggacctc gtcatcgtca acgaacgatc gacctcctat gtgcaggatc tgcagaacgc
    26761 gatcgacacc gccgtgcgca ccagccggtc gattcagcgc accggcccgg cgctggcgcg
    26821 cggttcgatc tatgtgctgc gcgccgacct gctgagcgtg caggcgcgcg ccatgttgcg
    26881 tgccgccgcg cgcgtcacgc tgatcgcacg ccgcgggccg ctcgctgccc agttcacccg
    26941 tctgccgcga ttgccggccg tcgtgccgcc tgcgcccgcg cgcgcgtcga cgcacaccgc
    27001 gacgacgcgg ctgcagcggc cggagttgga gttctacaac ggcctgggcg gcttcgataa
    27061 aaacggccgc gagtacgtgg tcgtgctcga cgcggaccgc accacgcccg cgccgtggat
    27121 caacgtcgtc gccaacccgg acttcggctt tcaggtttcg gcggagggca gcgcttatac
    27181 gtggtcggaa aacagccgcg agaatcagct cacgccctgg tccaacgatc cggtcaccga
    27241 tccgacgggc gaagccatct acgtgcgtga cgaagaaagc ctggagctgt ggagcccgac
    27301 cgcgcagccc atccgcgacg gcggcaccta catcgcgcgc cacggccacg gctacagccg
    27361 cttcgaacac cgggcgaacg gcattgcgct cgacctgttg cagtacgtgc cgctgtccga
    27421 tccgatcaag atttcgcgtc tgcgcttgcg caacctttcc ggacgacggc gccggctgtc
    27481 ggtcaccacc tatgcggaat gggtgctcgg tacttcgcgc ggcgcctccg caccgcgcgt
    27541 cctgaccgag atcgaccgag cgaccggcgc gctgttcgcg cgtaatccct gggcgatgaa
    27601 cttcggctcg cggatcgcct tcgccgatct gcgcggcgag caatcggcgt ggaccgcgga
    27661 tcgcacggag tttctcggcc gcaacggcac gctcgcggcg cccgcggcgc tcgcacgcga
    27721 cataccgctg tccggcgcaa caggcgcggg gctcgatcca tgcgccgcat tgcagcgcgt
    27781 gatcgaactc gaacccgacg agagcatcga agtcgtcgcc ttcctcggcc agtgcgagac
    27841 ggccgaggac gcgcacgcgc tgatcgcccg ctatcgcagc gccgatctgg acgcggtgta
    27901 cgacgaagtc gtcgatcatt ggcagacgct gctcggcacc atccaggtcc gcacgcccga
    27961 tcgcgcgatg gacatcatgc tgaacggctg gctgctctac caggcgctgg cttgccgcat
    28021 ctgggcgcgt tccgccttct accaggccag cggcgcctac ggcttccgcg atcaactgca
    28081 ggacggcatg gcgctcactt tcgcccgtcc cgaggcgacg cgccatcatc tactgcgcgc
    28141 cgcaggccgg cagttcgccg aaggcgatgt gcagcactgg tggctgccgc attccggcca
    28201 gggcgtgcgc acacggattt cggacgaccg cgtctggctc gcctacgcgg ccgcgaccta
    28261 tatcgccgcc acgggcgatg ccgccgtgct cgacgaacag atcccgttcc tcgaaggtcc
    28321 ggccgtgagc cagggcgaac acgatgcgtt cttccagccg atgaccgccg aagagtccgc
    28381 gtccctgttc gaacactgcg cgcgcggcct cgaccagtgt ctcgcgctga ccggaaagct
    28441 cggcctgccg ctgatcggca ccggcgactg gaacgacggc atgaaccgcg tgggcgaagg
    28501 cggcaaaggc gaaagcgtgt ggctcgcgtg gctcctgctg cgcaccatcg aactgttcgc
    28561 gccgtatgcg gacaagcgcg atcccgaacg cgcgcgccgc tggcgcgcgc acgccgaatc
    28621 ggtgcgccgg gcgatcgaga ccaaagcctg ggacggcgag tggtatcgac gcgccacttt
    28681 cgacgacggc gcgtggctgg gatcgaagga cagcgaggaa tgctgcatcg actcgatcgc
    28741 gcaaagctgg gcggtgctgt ccggcgccgc cgatcccgat cgtgcgcgca aggcgatggc
    28801 ctcgctcgaa caacacctga tccgccgcga cgacggcatc gccctgctgt tcacgccgcc
    28861 cttcgaccgc atggcgcgcg atccgggtta catcaaaagc tacccgcccg gcctgcgcga
    28921 aaacggcggg caatacagtc acgcggcgat gtgggtggtg ctcgcctttg ccaagctggg
    28981 cgaaggcgac aaggccggtg agttgttcgc gctgctcaac cccatcaatc actcgcgtac
    29041 gccggaggaa gccgagcgct acaaagtcga gccctatgtg gtcgcggccg acgtctactc
    29101 cgtcgctccg catgccggac gcggcggctg gacctggtac acgggttccg ccggctggat
    29161 gtatcgcgcc ggcatcgagg gcctgctcgg catccgccgt gaaggtgcga cgctcgtcat
    29221 cgatccatgt atccccgccg attggccggg attcgaggcc acggtgcgaa tcgcatcgac
    29281 ccgctgcgat atcgtcgtgg aaaacgcgtc gaagcgctgt cgcgacgttg cacacgcgac
    29341 ggtcgacgac gtaccggtcg attgcgagca cggcgtccgc gtatcgttga acggcggcac
    29401 gcaccgcata ttgatcacgc tgtagcgcgc cggcggcaac gctcagtgca gcacggcgca
    29461 gcgttgccgc attcacccct tcacttgcgc aatccgctgc ggctgacggt cctggcacct
    29521 ccggatcgag cgacaaatgc cctccattta gtgagggtca aatttgatct gggtccggtc
    29581 ggctaagatg tgcgtctcgg tccgcacttc tgcagcggcc cctctccatt cagcgaccgc
    29641 tcgcactcga gcagtcccgc aagcagtaac ctaggagcaa cacatgtaca gggctttact
    29701 tgcgagcgtc ggcgcaagcc tcgccgtctc gatggcgcac gcacagccaa ccgtcgcgcc
    29761 cgatatcgcg ctcggcaaga atctctccgt cacctgcgcc gcatgccatc agcccgacgg
    29821 cagcggacgc gacatcccgc agggcgaatc ctggccgcgg ctggccggca tgagtgccca
    29881 ctatctggcc aagcagatgc gcgatttcaa atcgggcgcg cgcaagagcg cgaccatgca
    29941 ggccttcgcg caaatgctcg acgatgcgca gatcgcacac gtctcggcgt attacgccag
    30001 cctgccaccg cccgcatccg caacaccggc cgaagccgac gccgccctgc tcgagcgtgg
    30061 caagaggctc gcgctgcaag gcgactggga ccgctacatc gttccctgca aaagctgcca
    30121 cggcccggat aaccagggcg ccggaccgtc ctttcccggc attgccggcc agcacgcgca
    30181 ttacatcagc gcacagttgc gggcctggca ggagggcacg cgggcgaacg acccgttgaa
    30241 tctgatggcg gccattgcga agcgaatgac cgccgaagac atccgcgcgg tatccgcgtg
    30301 gctcgcaacg cagccggccc gcgcgaaagg agcgaaatga tgacgaccca cgcacgacgc
    30361 aaaaacttcg cgcgtaagcg cgcgtggctc gcgctgctgc tcgcctgcgg catcggcgtg
    30421 aacgctcaag ccgatccgca gagcacggcc gaacgcttgg ctgcgatggc gcccgatgcc
    30481 cccgcgccca cggcagaaca gtacgtccac attccgccga ccctggagga cctggaagcg
    30541 tccaagctgc atccggaact caagcgcgtg atccggcgcg gacacgacat cttcgtcaac
    30601 acgcagcagt tccgcgacaa gtacgtgttc aacaacttga actgctcgag ttgccatctg
    30661 ggcgacgggc gcctgccctt ttccggtccc gtgtggccgg cagcggtgac gctgcccgac
    30721 taccggccga agaacgatca tgtgaacaac ctggaggaac gcattgccgg ctgcttctcc
    30781 tattcgatga acggcaaacc gcccgcctac ggcagcgacg acatgatcgc gctcgccacg
    30841 tacatccaat ggctcgccaa gggcgtgccg atgtatcagc cgggcagcag cctctacgga
    30901 cgcggttatc ccaggctcgc acaggcggcc gaacagccgg atgccgcgcg cggcaaggcg
    30961 atctacgccg agaaatgcgc gatctgccac ggcaacgacg gcgcgggtta tgtgcaaaac
    31021 ggcgaagtgg tctttcctcc gctgtggggc gacgactcct acaactgggg cgccggcatt
    31081 tcgcgcgtgt tcacgctcgc ggcgttcatc aagcacaaca tgccgctcgg tcagcccagc
    31141 agtctgtcgg atcaggaagc ctgggatctc gcgcaataca tcgacagccg cgagcggccg
    31201 caggatccgc gctataccgg gaacgtcaag gaaacgcgag cgaagtatct cgacacgttc
    31261 cacaagcaca cgctctacgg cgtcgaagtg gaaggcaaac tgctcggcga tcacaacaac
    31321 accggccgca agaattttct caagccggaa gtgctgcagc cgcggcatta caagtaaggc
    31381 tcgggccacg ccggcgtctg tgtgcggacg ccggcgtt
//
LOCUS       DATGOU010000009        11141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_6243423, whole genome shotgun sequence.
ACCESSION   DATGOU010000009 DATGOU010000000
VERSION     DATGOU010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11141
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6243423"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1657)
                     /locus_tag="VKZ48_00615"
     CDS             complement(68..1657)
                     /locus_tag="VKZ48_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HLU76385.1"
                     /translation="MIELFTDPTVWVGLLTLVVLEIVLGIDNLVFIAILASRLPPRQR
                     DMARIIGLSLALFMRLALLAAISWLVGLTAPLFHVGPMAVSGRDLILFLGGLFLLYKA
                     TTELHARLEGEDHHAGTDTRAYPSLWVVIAQIVALDAVFSLDSVITAVGMVNDLPVMM
                     AAVVIAIIVMLVASKPLTNFVNAHPTVVVLCLSFLLMIGLSLVADGLGFHLPKGYLYA
                     AIGFSIMIEVFNQIAQRNRLKKERRLPARQRAAEAILRLLAGPQALEAAPAVAGDAPA
                     MAPGEAAPFVPEERQMVQGVLTLAERSLRSVMTPRSHLAWINIEDDADAIRAQLRSRP
                     HSRYPIARGTLDRLLGIVRAKDAIYDLAHEGAIREDSIRKPMILSENMQALHAIHHLR
                     QAGTQLALVSDEHGVIQGCVTPLDVLEAIAGDFPEEGERPEIQPQGDGRWLIDGGADL
                     HHLEQVLETRGLVSEDERYTTIAGLMNARLQRTPTVGDSFRHENYEFRVTDVRHQRAH
                     AVEVTRIDEPGEEEMAQHAGA"
     gene            complement(1971..3347)
                     /locus_tag="VKZ48_00620"
                     /pseudo
     CDS             complement(1971..3347)
                     /locus_tag="VKZ48_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761826.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
     gene            complement(3416..3868)
                     /locus_tag="VKZ48_00625"
     CDS             complement(3416..3868)
                     /locus_tag="VKZ48_00625"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HLU76386.1"
                     /translation="MSARIPDFSLPSTGDKSFQLSKARGKYVVLYFYPKDNTPGCTLE
                     GQEFRDLHDEFVNAGCEIYGVSRDSIKSHENFKAKMGFPFELLSDADEEVCKLFDVIK
                     MKNMYGKQVRGIERSTFLLDPKGAIVHEWRGVKAAGHAQNVLDYIKAL"
     gene            complement(3966..4613)
                     /locus_tag="VKZ48_00630"
     CDS             complement(3966..4613)
                     /locus_tag="VKZ48_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76387.1"
                     /translation="MKQLCLWAAAAVLALAAHTASAFVITGTLDLDPRNPELDDNFDI
                     NVSIDVTGADALWTVSFISDQPTAFLGEFYFNIATGSYLFTNFDPDTWTVISPSTPVG
                     GGNWSADFLIEAHNTANGNTNRVFAGDALTFTMTNLDGDFTEATFLDAPCSTTTAFGG
                     CWQLGAHIQGFENLPPGSLFLVGNFDGGDNGVPTPGTLALLGASLLGFGLARRRL"
     gene            complement(4779..5813)
                     /locus_tag="VKZ48_00635"
     CDS             complement(4779..5813)
                     /locus_tag="VKZ48_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HLU76388.1"
                     /translation="MKLALRITASTLRGTREDVPRLANALLRQNANATFLFTLGPDRT
                     GRVLFRPRLRRDAAPDLRAHYGSRSLLYGTLLLGPSIGLRCRKILRRVRDDGFECGVH
                     GWDAVEWQHRIARADEVWTARQMHLAWGRYSELFGELPRVHGAAGWRMNRHALRLAQR
                     AGLDYASDTRGRSPFLPVREGELIACPQLPTTLPTLDELMRQGLNPEAAVDELLALAR
                     KAPATGHVYSAAVEREGARLLPLFERLLDGWQALGFELTALRTLYDSLDRGHLPHCLL
                     EEGTVPGRTSPVLLQGKAYLDGATDRVVLPERRRYPRPASGYLLPRRVDEPNRPPREP
                     RADEPRGLRL"
     gene            complement(5839..6210)
                     /locus_tag="VKZ48_00640"
     CDS             complement(5839..6210)
                     /locus_tag="VKZ48_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HLU76389.1"
                     /translation="MKFHLTQAAGRNLITAYGPGYVMVNQDRHEQSLIVMAEELVTDW
                     PPGSVADLQPDHMRTLTEWGLEVVLIGTGPTLRFPGPEIMRPLMEARLGFEVMDVHAA
                     CRTYNILVDEGRRVAAALLLA"
     gene            6387..7646
                     /locus_tag="VKZ48_00645"
     CDS             6387..7646
                     /locus_tag="VKZ48_00645"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HLU76390.1"
                     /translation="MTANPDRLLRAVPQPVRKSAKLANVCYDIRGPVLARARQMEEEG
                     QRIMKLNIGNLAPFGFEAPDEVVQDVIFNLPNACGYTDSKGLFAARKAVMQYAQEKRI
                     PGVTLDDIIIGNGVSELIVMAMQGLLDNGDEVLVPAPDYPLWTAAVALSGGVARHYMC
                     DEGAGWLPDLDDIRAKIGPRTRAIVLINPNNPTGALYPDDLLEEIVEIARQHELIVYA
                     DEVYDKTLYDGAKHTSIASLADDVLFVTFNGLSKNYRACGYRTGWMIISGAKQHALDY
                     IDGLNILASMRLCANVPGQYAVQTALGGYQSINDLVAPNGRLTRQRDLAYRLLTDIPG
                     VTCVKPQAALYLFPKLDPKVYPIRDDQEFILQLLEQEKVLVVQGTGFNWPDPDHLRIV
                     FLPNADDLTEAMERLSRFLDQYRRKHA"
     gene            7643..8086
                     /locus_tag="VKZ48_00650"
     CDS             7643..8086
                     /locus_tag="VKZ48_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLU76391.1"
                     /translation="MSMNIVDVTDADGEVLHVALLARAEPVHRQLRPDLPPDYVTKMR
                     RVFAGGGRMSVVLVDAEVRGVAVYRAFENTSDDAKFYIDDIVVDTVARSQGVGSALMA
                     HLQKKARLLNCKTFALDSGTQRHDAHRFYFREGMSIMSFAFKKQL"
     gene            8100..9416
                     /locus_tag="VKZ48_00655"
     CDS             8100..9416
                     /locus_tag="VKZ48_00655"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HLU76392.1"
                     /translation="MKPINVGLLGIGTVGGGTWNVLKRNREEITRRAGREIRIVAVAD
                     KDAEKAKRLTNGDAPVLADAMAIVNDPNIDIVVELIGGYTVAKDLVLQAINNGKHVVT
                     ANKALLAVHGNEIFKAAQEKGVMVAFEAAVAGGIPIIKALREGLTANRIEWIAGIING
                     TSNFILSEMREKGLPFDDVLKEAQRLGYAEADPTFDIEGVDAAHKLTIMSAIAFGIPM
                     QFDKAYTEGISKLTREDIQYAEKLGYRIKLLGIAKHVEKGIELRVHPTLIPAKRLIAN
                     VEGVMNAVVVKGDAVGTTLYYGAGAGAEPTASAVVADLVDITRLHTADPDNRVPHLAF
                     QPDRLSDIPVLPVEEVTTAYYFRMRVQDQPGVLADITRILAERSISIDAMMQREPDEG
                     EDQTDIIMLTHEAQEKHVNAAIEAIEALPVVHGKITRIRLEELGKN"
     gene            9599..11008
                     /gene="thrC"
                     /locus_tag="VKZ48_00660"
     CDS             9599..11008
                     /gene="thrC"
                     /locus_tag="VKZ48_00660"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HLU76393.1"
                     /translation="MKYISTRGGMKPARFTEILLEGLAPDGGLAIPESYPQIDRATLA
                     KWRHLSYRDLAFEILSLYIDDIPAADLKDIIDRTYTVEAFGTEDITPLHKLEDGLYLL
                     QLSNGPTLAFKDMAMQLLGNLFEYVLAKEGKQLNILGATSGDTGSAAEYAMRGKNNIR
                     VFMLSPHGKMSPFQKAQMYSLQDPNIHNIAVKGVFDDCQDIVKAVSNDLAFKQRYNIG
                     TVNSINWARLIAQVVYYFKGYFAATTSEDEQVSFAVPSGNFGNVCAGHVARMMGLPIK
                     QLIVATNENDVLDEFFRTGTYRVRKGKDVYETSSPSMDISKASNFERFVFDLVGRDPQ
                     KVKALWEQIEREGAFDLAQTGDFARVKDFGFVSGRSTHADRLDTIRMADRDYNTTIDT
                     HTADGLKVGLAHREPGVPLICLETALPAKFEATIQEALGRPPKRPLGYEDIEKLPQRC
                     EVMDADAAAVKQYIQKHAA"
BASE COUNT         2061 a         3559 c         3567 g         1954 t
ORIGIN      
        1 tcggcagcag ggtcaaaggt tcacgcgcgc gacacgggta atcgaaggcc gcgtgtcggt
       61 caaacgatca ggcgccggcg tgctgcgcca tctcttcctc tccgggctcg tcgatgcgcg
      121 tcacttccac cgcatgcgcg cgctgatggc gcacgtccgt gacgcggaac tcgtagtttt
      181 cgtgcctgaa gctgtcgccc accgtgggcg tgcgttgcag ccgcgcattc atcagcccgg
      241 cgatggtggt gtagcgctcg tcctcgctca ccaggccgcg cgtttccagc acctgctcca
      301 gatgatgcag atccgcgccg ccgtcgatca gccagcggcc gtcgccctgg ggctgaatct
      361 cgggtctttc gccttcctcg ggaaaatcgc cggcaatcgc ctcgagcaca tcgagcggcg
      421 tgacgcatcc ctgaatgacg ccgtgctcgt cgctcaccag cgccaactgc gtgcccgcct
      481 ggcgcaagtg gtgaatggcg tgcagcgctt gcatgttctc cgacaggatc atcggcttgc
      541 ggatgctgtc ctcgcgaatg gcgccttcgt gagcgaggtc gtagatcgcg tccttcgccc
      601 ggacgatacc gagcagacgg tccagcgtgc cgcgtgcgat gggatagcgg ctgtgcgggc
      661 gcgagcgtaa ctgcgcgcgg atcgcgtcgg cgtcgtcttc gatgttgatc caggccaggt
      721 gcgaacgcgg cgtcatcacc gagcgcagcg agcgctccgc cagcgtgagc acgccttgca
      781 ccatctgccg ctcctccggt acgaacggcg cggcctcgcc cggtgccatc gcaggcgcgt
      841 cgcccgccac ggccggtgcg gcctccagcg cctgcggtcc ggccagcagc cggaggatcg
      901 cctcggcagc acgttgtcgt gcaggcaggc gccgctcttt cttgaggcgg ttgcgctgcg
      961 cgatctggtt gaacacctcg atcatgatcg agaagccgat cgcggcgtag agataaccct
     1021 tgggcagatg gaagcccagg ccgtcggcca ccaggctgag accgatcatg agcaggaagc
     1081 tgaggcacaa cacgaccacc gtcgggtgcg cgttgacgaa gttcgtaagc ggcttcgacg
     1141 cgaccagcat gacgatgatc gcgatgacga ccgcagccat catcaccggc agatcgttca
     1201 ccatgcccac cgcggtgatc accgagtcga gcgagaacac ggcgtccagc gcgacgatct
     1261 gcgcgatcac gacccacagg ctgggatatg cccgggtgtc tgtcccggcg tggtgatcct
     1321 cgccttccag gcgcgcatgc agttcggtcg tggccttgta aagcaggaac aggccgccga
     1381 ggaagaggat gagatcgcgg ccggacacgg ccatcggccc cacatggaac agcggcgcgg
     1441 tcagtccgac cagccacgag atcgcggcga gcagcgcaag ccgcatgaac agcgcgagcg
     1501 acaggccgat gatgcgcgcc atgtcgcgtt gtctgggcgg cagccgcgac gccaggatgg
     1561 cgatgaacac caggttgtcg atgccgagca cgatctcgag cacgacgagg gtcagcagac
     1621 ccacccacac ggtcggatcg gtgaacagtt cgatcatgag acctctgttt ggcgaacgcc
     1681 cggaatgcgg gcaaggatag ccccattcgc ttgcaggcag cgggccgtga cggcgacagg
     1741 cggctcggcg cgctgttcaa tccttcgaag cgcccggagc gggaagaagc tcaaccgttc
     1801 gtggtgagcg cgagcgcagc gagcagtcga accatgagcg gccgggagca gaaggatgat
     1861 gaccgaccgg gccgttcatc cttcgacccg ccctgcgggc ggctcaggac gaacggcagc
     1921 gactctccgc gcgcacttct ccgccttcag ttctccactc tcgactcgac ctacagcacg
     1981 tccgccgcgt aatccgccag acgcgagcgt tcgccgcgct gcagcgtcac gtggcccgaa
     2041 tggcgccagc ccttgaagcg atccaccacg taggtcagac ccgaactgcc ttcggtgagg
     2101 tagggcgtgt cgatctgcgc gatgttgccc aggcacacca ccttcgtgcc gggaccggcg
     2161 cgggtgatga gcgtcttcat ttgtttcgac gtcaggttct gcgcttcgtc gatgatgagg
     2221 aacttgttga ggaaggtgcg gccgcgcatg aagttgagcg acttcacctt gatgcgcgag
     2281 cggatgagat cgcgtgttgc ggcgcggccc cagtcgccgg cggcctcgtc gggcgtgttg
     2341 agcacgtcca ggttgtcttc cagcgcaccc atccacggcg tcatcttttc ttcctcggtg
     2401 ccggggagga agccgatgtc ctcgcccacc ggcacggtca cgcgggtcat gatgatttcg
     2461 ctgtagagct tgtcctccag cgtctgcatg agaccggcgg ccagcgtcag cagcgtcttg
     2521 cccgtgccgg cctggccgag cagggtgacg aagtcgatgt ccgggttcat cagcaggttg
     2581 aaggcgaagt tctgctcacg gttgcgcgcg gtgatgcccc acactgcatt cttgctgtgg
     2641 ctgtagtcgc gcaccgtttc cagcacgacg ctgcccggct cctgttcgcg cacccaggct
     2701 tgcagcggct gatcgccttc ctgatagacg aactcattga gcagcaggct ttgcgtgagc
     2761 gggcccttca gcttgtagta ggtgcggccg tccttcttcc aggactccat gtccttgccg
     2821 tgctgctccc agaagtcagc cggaagttcg cgcacgcccg catagaggac gtccgtgtcc
     2881 tccagcacct tgtcgttgaa gtagtcctcc gccgcgagac cgaccgcacg cgccttgatg
     2941 cgcatgttga tgtctttgga caccaggatc acctggcgcg ccggatggcg ttcctgcagg
     3001 aacatcacca ccgcgatgat gtgattgtcg gccttgctgt tgggcaggct cggcggcagc
     3061 gtggcggcga tggcttcggt ctgcagatag aggcggccgg tggcggtatc ggcatcgtgg
     3121 cgcttgagcg gaatgccttc gctcagcggc acggttgcgc cgctgacgat ctcgtccaga
     3181 tagcggctgg cctgtcgcgc gttgcgggcg acttcggaga cgcccttctt gttgttgtcc
     3241 agttcctcca gcgtcatcat cggcacgtag atgtcgtgct cctcgaagcg gaagaggctg
     3301 gtcggatcgt gcatcagcac gttggtatcg agtacgaaaa gcttggtctg cccggtgttc
     3361 ttgtcgtcgc gcggttttct ggtggccatg ttgctttctt gtaggtttgc tgcggctaga
     3421 gcgccttgat gtagtcgagc acgttttgcg cgtgtccggc tgctttcacc ccacgccatt
     3481 cgtgaacaat ggcgcctttg ggatccagca ggaaagtgct gcgctcgatg ccgcgcacct
     3541 gcttgccgta catgttcttc attttgatga cgtcgaacag cttgcagact tcctcgtccg
     3601 catcggagag cagttcgaag ggaaaaccca tcttggcctt gaagttctcg tgcgacttga
     3661 tgctgtcgcg cgagacgccg tagatctcgc agcccgcatt gacgaattcg tcgtgcagat
     3721 cgcggaattc ctgtccttcg agggtgcagc ccggcgtgtt gtccttgggg tagaaataga
     3781 gcacgacgta cttgccgcgt gctttggaca attggaagct tttgtcgccc gtgctcggga
     3841 gactgaaatc gggaatgcgt gctgacatga tggcaaagag cgctgaatca gccgcgattg
     3901 ttgcgggatt cccgaggcga cgcaaccgcg gacgcgaagc cggcgcttcg cgtccgcggg
     3961 ccgaactaga gacgacgacg cgcgagcccg aatccgagca gcgatgcgcc gagcaacgcc
     4021 agcgtgccgg gcgtcggaac gccgttatcg ccgccgtcga aattgcccac caggaacaga
     4081 ctgccgggcg gcaggttctc gaagccctgg atatgcgcgc cgagttgcca gcagccgccg
     4141 aaggccgtcg tcgtcgagca gggcgcatcg aggaaggtcg cttcggtgaa atcgccgtcg
     4201 aggtttgtca tggtgaacgt cagcgcgtcg ccggcaaaca ctctgttggt gttgccgttt
     4261 gcggtgttgt gcgcctcgat caggaagtcg gccgaccagt tgcctccgcc gaccggcgtc
     4321 gaaggcgaga tcaccgtcca ggtatcggga tcgaagttgg tgaagagata tgaacccgtt
     4381 gcgatattga aataaaactc gccgaggaat gcggtcggct ggtcgctgat gaaactcacg
     4441 gtccacaaag catccgcgcc cgtgacatcg atcgagacgt tgatatcgaa gttgtcgtcg
     4501 agttcgggat tgcgcggatc gaggtccagc gtcccggtga tgacaaaggc tgaagcggtg
     4561 tgggcggcca gtgcaagaac ggcggccgcg gcccaaaggc aaagttgctt catgaaaatc
     4621 cccttgagat aagtgcatgc tgcgcacaag gggtgtgccg cacaaaacat accgggttgt
     4681 ctagaacatt gttttgtctt cgaaaattac ccttttgaca cgtgttgccc ggcattttgt
     4741 caacgacacc gacagttttg ttggtcgttg gggacagttc agagccgcaa gccgcggggc
     4801 tcgtccgccc gcggttcgcg cggcggccgg ttgggctcgt ccacccggcg cggcagcaga
     4861 tagccgctcg ccgggcgcgg atagcgccgc cgttcgggca gcaccacccg atccgtcgcg
     4921 ccgtccagat aggccttccc ctgcagcaat acgggtgagg tacgtcccgg cacggtgcct
     4981 tcctcgagca ggcagtgcgg cagatggccg cgatcgagcg agtcgtacag cgtgcgcagc
     5041 gcggtgagtt cgaaaccgag cgcctgccac ccgtcgagca ggcgctcgaa cagcggcagc
     5101 agtcgcgcac cttcgcgttc gacggccgcg ctatagacat gccccgtcgc gggcgccttg
     5161 cgcgccagcg caagcaactc gtccaccgcc gcctcaggat tcaggccctg ccgcatcaac
     5221 tcgtccaggg tcgggagcgt ggtgggcaat tgcgggcagg cgatcagttc accctcgcgc
     5281 accggcagaa agggcgagcg gccgcgcgta tccgaggcgt aatccagccc cgcgcgctgc
     5341 gccaggcgca gggcgtggcg gttcatgcgc cagccggcgg cgccgtgcac gcgcggcaat
     5401 tcgccgaaca gttcgctgta gcgcccccag gcgaggtgca tctgtcgggc ggtccagacc
     5461 tcgtcggccc gggcgatccg gtgctgccat tcgacggcgt cccagccgtg cacgccgcat
     5521 tcgaagccgt cgtcgcgcac gcgccggagg atcttcctgc agcgcaggcc gatgctcggc
     5581 ccgagcagca gcgtgccgta caggaggctg cgcgagccgt aatgcgcgcg caggtcggga
     5641 gcggcgtcgc gccgcagccg cgggcgaaag agcacgcggc cggtgcggtc gggcccgagc
     5701 gtgaacagga aggtggcgtt cgcgttctgc cgcagcaggg cgttggccag gcggggaacg
     5761 tcctcccggg tgccgcgcag ggtggaggcg gtgatgcgca gggcgagctt catgcgcgcc
     5821 catgcggcgc gggcggggct aggcgagcag cagcgcggcg gcgacccggc gcccttcgtc
     5881 caccaggatg ttgtaggtgc ggcaggcggc gtggacgtcc atgacctcga agcccagacg
     5941 ggcctccatc agcggccgca tgatttcggg gccggggaag cgcagcgtgg gtccggtgcc
     6001 gatcagcacg acttccagac cccattccgt cagggtgcgc atgtggtcgg gctgcagatc
     6061 cgcgacgctg ccgggcggcc agtcggtgac cagttcctcg gccatgacga tcaggctctg
     6121 ttcgtggcgg tcctgattga ccatcacgta acccgggccg taggcggtga tcagattgcg
     6181 tccggcggct tgtgtgagat gaaacttcaa atcgtcgtcc tgaaagtggc caaattatag
     6241 cgtctggacg tacgactgca tggagcatgg ggcgtttcac ggaaatccgt tttgttcaat
     6301 tgcggctgga ggttgtagcg actaccattg ccttcttttg cgctgcagcg tagcaatttc
     6361 gctgccaccc tccgtttaag cctccaatga ccgccaatcc cgaccgtctg ctccgcgccg
     6421 tgccgcagcc cgtgcgcaaa tcggcaaagc ttgccaacgt ctgctacgac attcgcggtc
     6481 cggtgctcgc gcgcgcgcgc cagatggagg aggaagggca gcgcatcatg aagctgaaca
     6541 tcggcaatct cgcgccgttc ggcttcgaag cgcccgacga agtggtgcag gacgtaatct
     6601 tcaacctgcc caacgcgtgc ggctataccg attccaaggg cctgttcgca gcgcgcaagg
     6661 cggtgatgca gtacgcgcag gagaagcgca ttcccggcgt gacgctggac gacatcatca
     6721 tcggcaacgg cgtgtccgag ctgatcgtga tggccatgca gggcctgctc gacaacggcg
     6781 acgaagtgct ggtgcccgca cccgattacc cgctatggac cgcggcggta gcgctttccg
     6841 gcggcgtggc gcgtcactac atgtgcgacg aaggcgccgg ctggctgccc gatctggacg
     6901 acatccgcgc gaaaatcggc ccgcgtacgc gcgccatcgt gctgatcaac ccgaacaacc
     6961 cgaccggcgc gctgtatccg gacgacctgc tcgaggaaat cgtcgagatc gcccgccagc
     7021 acgagctcat cgtttacgcc gacgaggtgt acgacaagac gctgtatgac ggcgccaagc
     7081 acacgtccat cgcctcgctg gccgacgacg tgctgttcgt gaccttcaac ggcctgtcga
     7141 agaactaccg cgcctgcggc taccgcacgg gctggatgat catctcgggt gccaaacaac
     7201 acgccctgga ttacatcgac ggtctgaaca tcctcgcgtc aatgcgcctg tgcgccaacg
     7261 tgccggggca gtacgccgtg cagaccgcgc tgggcggcta tcagagcatc aacgatctgg
     7321 tcgcgcccaa cggacggctg acgcgccagc gcgatctggc ctacaggctg ctcaccgaca
     7381 ttcccggcgt cacctgcgtc aagccgcagg cggcgctgta tctgttcccg aaactggacc
     7441 cgaaggtcta tcccatccgg gacgaccagg aattcatcct ccagttgctc gagcaggaga
     7501 aggtgctggt cgtgcagggc acgggtttca actggcccga tccggaccac ctgcgcatcg
     7561 tgttcctgcc caacgccgac gacctcaccg aggccatgga acggctgtcg cgttttctgg
     7621 accagtatcg ccgcaagcac gcatgagcat gaatatcgtc gacgtgaccg acgccgacgg
     7681 cgaagtgctg catgtggcac tgctggcgcg cgcggagccg gttcatcgtc agttgcgccc
     7741 cgacctgccg ccggattacg tcaccaagat gcggcgcgtg ttcgccggcg gcggccgcat
     7801 gagcgtcgtg ctggtggatg cggaagtgcg cggcgtggcc gtctatcgcg cgttcgagaa
     7861 caccagcgac gatgcgaagt tctatatcga cgacatcgtt gtcgatacgg tggcgcgttc
     7921 acagggcgtg ggcagtgcgc tgatggcgca tctgcagaag aaggcgcgtc tgctcaactg
     7981 caagaccttc gccctcgatt cgggcacgca gcggcacgac gcacaccgat tttatttccg
     8041 cgagggcatg agcatcatgt ccttcgcgtt caagaaacag ttgtagggcg ttaatcagaa
     8101 tgaaaccgat caacgtagga ttgctcggca tcggcactgt cggtggcggg acctggaacg
     8161 tactcaaacg caaccgcgag gaaatcaccc gccgtgcggg ccgcgagatc cgcatcgtcg
     8221 cggtggcgga caaggacgcg gagaaagcca agcgcctgac caacggcgac gcgccggtgc
     8281 tcgccgacgc catggcgatc gtcaacgacc cgaacatcga catcgtggtc gaactgatcg
     8341 gcggctacac cgtggcgaag gacctggtgc tccaggccat caacaacggc aagcacgtcg
     8401 tcaccgccaa caaggcgctg ctcgccgtgc acggcaacga gatcttcaag gctgcgcagg
     8461 agaagggcgt catggtcgcc ttcgaagcgg cggtcgccgg cggcatcccg atcatcaagg
     8521 cgctgcgcga aggcctcacc gccaaccgca tcgaatggat cgccggcatc atcaacggca
     8581 cctcgaattt catcctttcg gaaatgcgcg agaagggtct gcccttcgac gacgtactca
     8641 aggaagcgca gcgcctgggc tatgccgagg ccgatccgac cttcgacatc gaaggcgtgg
     8701 acgccgcgca caagctgacc atcatgtcgg ctatcgcctt cggcattccg atgcagttcg
     8761 acaaggccta cactgaaggc atttccaagc tcacgcgcga ggacatccag tacgcggaga
     8821 agctcggcta ccgcatcaag ctgctgggca tcgccaagca cgtggagaag ggcatcgagc
     8881 tgcgcgtgca tccgacgctc attccggcca agcgcctcat cgccaatgtg gaaggcgtga
     8941 tgaacgccgt ggtggtgaag ggcgacgccg tcggcacgac gctgtactac ggcgcgggcg
     9001 ccggcgcgga accgaccgcc agcgccgtcg tggccgacct ggtggacatc acccgcctgc
     9061 ataccgccga tccggacaac cgcgtgccgc atctggcctt ccagccggat cgcctgtccg
     9121 acattcccgt gctgccggtg gaggaggtga cgaccgccta ctacttccgc atgcgcgtgc
     9181 aggatcagcc cggcgtgctg gccgacatca cgcgcatcct cgccgagcgc tccatctcca
     9241 tcgacgccat gatgcagcgc gagcccgacg agggcgagga ccagaccgac atcatcatgc
     9301 tcacgcatga agcgcaggag aaacacgtca acgcggccat cgaggcgatc gaagcgctgc
     9361 cggtcgtgca cggcaagatc acgcgtatcc gtctggaaga actcggcaaa aactaaaacc
     9421 ttgaaccgca gagacacaga gaagttaaaa gctttgccac caagacacga agggcaccaa
     9481 ggaggcacca agaactgctg taacgaagct ggttccttgg tgccttggtg gttcaaagag
     9541 tttgcttttc tctgtgttct cggtgtctct gtggtaagga tgttgtaggt ttcgactgat
     9601 gaaatacatc tccacccgcg gcggcatgaa gcccgcccgc ttcaccgaaa tcctgctgga
     9661 aggcctggcg cccgacggcg gcctggccat acccgagtcg tatccgcaga tcgaccgcgc
     9721 caccctggcc aaatggcggc acctgagcta ccgcgatctg gccttcgaga tcctgtcgct
     9781 ctacatcgac gacattcccg ccgccgacct gaaggacatc atcgaccgca cctacacggt
     9841 cgaagccttc ggcaccgagg acatcacgcc gctgcacaag ctggaagacg gcctctacct
     9901 gctgcagctc tccaacggcc cgacgctcgc cttcaaggac atggcgatgc aactgctcgg
     9961 caacctgttc gagtacgtgc tggcgaagga gggcaagcag ctcaacatcc tcggcgccac
    10021 ctcgggcgac accggctcgg ccgcggaata cgccatgcgc ggcaagaaca acatccgtgt
    10081 cttcatgctc tcgccgcacg gcaagatgag cccgttccag aaggcgcaga tgtattcgct
    10141 gcaggacccg aacatccaca acatcgccgt caagggcgtg ttcgacgact gccaggacat
    10201 cgtcaaggcc gtgagcaacg atctggcgtt caagcagcgc tacaacatcg gcacagtgaa
    10261 ctccatcaac tgggcgcgcc tcatcgccca ggtggtgtac tacttcaagg gctacttcgc
    10321 cgccaccacg agcgaggacg agcaagtgag ctttgcggtg ccctcgggca acttcggcaa
    10381 cgtctgcgcc ggccacgtcg cccgcatgat gggtctgccg atcaagcagc tcatcgtcgc
    10441 caccaacgag aacgacgtac tcgacgaatt cttccgcacg ggcacctacc gcgtgcgcaa
    10501 gggcaaggac gtgtacgaga cttccagccc gtcgatggac atctccaagg cctcgaactt
    10561 cgagcgtttc gtgttcgatc tcgtgggccg cgacccgcag aaggtcaagg cgctgtggga
    10621 gcagatcgag cgtgagggcg ccttcgacct cgcccaaacc ggcgacttcg cccgcgtgaa
    10681 ggacttcggc ttcgtctccg gccgcagcac gcacgccgac cggctcgaca ccatccgcat
    10741 ggcggatcgc gactacaaca ccaccatcga tacccacacc gccgacggcc tcaaagtggg
    10801 cctcgcgcat cgcgaacccg gtgtgccgct catctgcctg gaaacggcgc tgcccgcgaa
    10861 attcgaggcg accatccagg aagcgctcgg ccgtccgccc aagcgcccgc tcggttacga
    10921 ggacatcgag aagctgccgc agcgctgcga ggtaatggac gccgacgcgg ctgccgtgaa
    10981 gcaatacatc caaaaacacg ccgcatgacc gcagtccaga atcgacccga ccggcaggaa
    11041 ggcttcgcgc agaccatcag agggtggaaa agcgactatc tttcgcgctg cctgatcgcc
    11101 ccggatgtaa acgaagtcgc caaggaggcg atccgccagg a
//
LOCUS       DATGOU010000010        20754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_22871, whole genome shotgun sequence.
ACCESSION   DATGOU010000010 DATGOU010000000
VERSION     DATGOU010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 20754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20754
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22871"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VKZ48_00665"
     CDS             <1..581
                     /locus_tag="VKZ48_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HLU76394.1"
                     /translation="MDSLTRYAMAQREIALAIGEPPATKGYPPSVFAKLPQLVERAGN
                     GAPGGGSITAFYTVLTEGDDLQDPIADSARAILDGHIVLSRSLADQGHYPAIDIEASV
                     SRVMHALSSEEAMQTVREFKSLYSRYQRNRDLVNVGAYVPGSDPVLDQALALYPQMEA
                     FLQQRMDERAPMAAGQAALATLFAQAQPAPAA"
     gene            752..1195
                     /gene="fliJ"
                     /locus_tag="VKZ48_00670"
     CDS             752..1195
                     /gene="fliJ"
                     /locus_tag="VKZ48_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HLU76395.1"
                     /translation="MQTDPMQTLQELAEDATREALCRLSESMKSCRESESRLELLRRY
                     RDEYRIKLDSATRTGVSALELSNFRNFLAKLDEAIAQQNADVAHWQAAVTRSRADWQE
                     AERRARSYGVLNERRAVQARGREARVEQKQNDEYAARAVASPRWG"
     gene            1202..2176
                     /locus_tag="VKZ48_00675"
     CDS             1202..2176
                     /locus_tag="VKZ48_00675"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HLU76396.1"
                     /translation="MSALHIVTTAIAPAATQSLSSAEESAETPFTALLGEQMQAHAQL
                     PAQLLSQAAEKQDTDTTEAEEFEAPELTAADAQALTAALPVEARQDALPARLFPERGT
                     ETLRTDVTARDGRNDRTGPRGPAAATSVPLAGDAAPETAGTTSRVAPAFELPAAQAIE
                     TPNPTGAAQAPVTTPAIERPAVATPVHTLQQPVGSARWAGELGQTVQILIRSEQQSAT
                     LHVTPADLGPIEVRIDMSGDQANLSFTVQHPDTRNVLENALSRLRDMLAEGGIALGDA
                     HVNQQSGEQREAAAEPAHRNGGGNALAPDTAAETIAVRARVGLVDTFA"
     gene            2426..2947
                     /locus_tag="VKZ48_00680"
     CDS             2426..2947
                     /locus_tag="VKZ48_00680"
                     /inference="COORDINATES: protein motif:HMM:NF015693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HLU76397.1"
                     /translation="MATQAQPAAQTPPTSPTPRKSRKKLWFTLTLLLLIAGMGGAGLM
                     LLIQPSTPMSEEEALAAEEAKRKARPPVFLALEPFVVNLRDEDGDHYLRLGVVLEGTN
                     QDALDVAKQQLPRIRNGILLLLSAKTSAEIATMEGKQRLMEEIIVAARAPMTLPEPSR
                     GIESVYFSDFVIQ"
     gene            2969..3967
                     /gene="fliM"
                     /locus_tag="VKZ48_00685"
     CDS             2969..3967
                     /gene="fliM"
                     /locus_tag="VKZ48_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HLU76398.1"
                     /translation="MSEFLSQEEVDALLKGVSGETDEPVEDTPTEGPRPYNLATQERI
                     VRGRMPTLEIINERFARLMRLGLFNFMRRNPDVSVGPVKVQKYNEFLRNLVVPTNINI
                     MQLKPLRGACLMVFDPSLIFAVVDTMFGGDGRFHTRVEGRDFTATEQRIIQRMLNVVV
                     DDYKKSWAPVYPLQMEYVRSEMHTQFANIATPSEVVISTSFDVEFGSSGGSMHICIPY
                     SVVEPIREVLYSPTQGDSYEPDKRWLRMLSRQVQIAEVELVANLAHVPTTLGSLLQMK
                     PGDVIPLELPQNVVAEVDGVPIFDCKYGTLNGHHAIRIEKVLAVSAKENNLGEDNG"
     gene            3960..4370
                     /gene="fliN"
                     /locus_tag="VKZ48_00690"
     CDS             3960..4370
                     /gene="fliN"
                     /locus_tag="VKZ48_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HLU76399.1"
                     /translation="MADSVDDDWGAALAEQAAASADAPAAAPAAKVFEPLASSSGDTG
                     TQDGLAHDIDLILDIPVQLTVELGRTKIPIKNILQLAQGSVVELDGLAGEPMDVLVNG
                     CLIAQGEVVVVNEKFGIRLTDIITPSERIRRLSK"
     gene            4367..4795
                     /gene="fliO"
                     /locus_tag="VKZ48_00695"
     CDS             4367..4795
                     /gene="fliO"
                     /locus_tag="VKZ48_00695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HLU76400.1"
                     /translation="MILSRIIATLCALAATPALAQGTAAPAAELAPAMPLGAMLQSLF
                     ALLVVLGVIVGAVWLLKRTQRLQGAGNEVLRNVAAVAVGPKERVVVVEVGDTWLVLGV
                     APGQVRSLYSLPRQNLPARGINAPKFSDWLTRARGGDERA"
     gene            4788..5546
                     /gene="fliP"
                     /locus_tag="VKZ48_00700"
     CDS             4788..5546
                     /gene="fliP"
                     /locus_tag="VKZ48_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462667.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HLU76401.1"
                     /translation="MRNRFISPAWLAAVALLALAPLPALAQNAGIPAFTSTPTPGGGQ
                     SYTLSIQTLLALSALTFLPAVLLLMTSFTRIIIVLSLLRHALGTQTSPPNQVLIGLAL
                     FLTLFVMGPTLDRVYNEAYLPYSESRMSFNDALARAESPMKDFMLRQTREADLALFLK
                     LADHPAVESPQEVPFRVLVPAFVTSELKTAFQIGFLVFIPFLIIDMVVASVLMSMGMM
                     MLSPVLISLPFKLMLFVMVDGWNLLLGSLSASFG"
     gene            5560..5829
                     /gene="fliQ"
                     /locus_tag="VKZ48_00705"
     CDS             5560..5829
                     /gene="fliQ"
                     /locus_tag="VKZ48_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HLU76402.1"
                     /translation="MTPQTVLTIGQQALQMTLMIAGPLLLTALLVGLVVSIMQAATQI
                     NEMTLSFIPKLVGLFVVLIIAGPWMLTTMTEYMRRLFQSIPFVIG"
     gene            5931..6695
                     /gene="fliR"
                     /locus_tag="VKZ48_00710"
     CDS             5931..6695
                     /gene="fliR"
                     /locus_tag="VKZ48_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HLU76403.1"
                     /translation="MISFTSEQVALWLGMYLWPFARVLALFMTAPVLHDQAVPARVKI
                     GFSLLAALLIAPLLPATQHVPISSPHAALVLAQQVLIGASIGFAVRLVFAALEMAGDV
                     IGLQMGLSFAGFISPGTGEQTPIVGSFFGVIASLVFLAINGHLMLLAAVTESFYALPI
                     TNGLGPQVNVAAMASAGSEVFRLGLHVALPVLATMLILNLALGVLARVAPQLNIFAIG
                     FPATLLVGMIALMLALPVLGPLLEQMLSQSLLPPLR"
     gene            complement(6752..7600)
                     /locus_tag="VKZ48_00715"
     CDS             complement(6752..7600)
                     /locus_tag="VKZ48_00715"
                     /inference="COORDINATES: protein motif:HMM:NF038125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="THxN family PEP-CTERM protein"
                     /protein_id="HLU76404.1"
                     /translation="MRNWAGLLAIAAALAITAPAQAAPVASWNYSISFAFTDADYTPG
                     IGTPFTSPFELSWGTTGGSFEVPDGDRSALTIGTDATNLTGGGVVNGTVTTNALTLPG
                     IGLSNTITHWNSPISSTFATLTGGQITSSLALESDGGIAVPGLPDLVFDFLFVETLNN
                     PPGNPGCAENTAEPCADIFVLVSSTAFNVPFTFDGIDYLISIFPFDAGGMLGSIATLG
                     AEQCEAAGAAPGCVGIITNEDEATTIQFAFAITTEPLLVPEPGMLGLLALGLFGLALV
                     RRSRAD"
     gene            complement(7800..12041)
                     /locus_tag="VKZ48_00720"
     CDS             complement(7800..12041)
                     /locus_tag="VKZ48_00720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLU76405.1"
                     /translation="MRRRFIVGYVLTACALLILAGWIIGLELEDRIAERAREAALLTK
                     TVEANLSYQIDRSGQVLARFASEGRSLAGDGGEADQAVIRNILDTRGTAIRPVFLIER
                     NGRVHLVPQAEGAPGVAAFEGLLAWHAQHEHVASRMAPTPLRAYGQTMIPITRRIVRQ
                     DGAFVGVVGMLLTTKLIEDIHQKLELSASRVEVHTADGELMIRLPPDAAPIAPQQSVT
                     QRMQIGALEVAHGGQTLQAARLVNDSPLRVVVSSSLADVYRAWREQALKVAVGLVLVL
                     LFTAGLIRALLEKLQRVEQSSAELDITRFTLDQTSDAIYWVDANGRFAFANPAAARWF
                     GVLPERLIGMPFVNIFPAVTPERFAHSFDALRGVGERVYRMEYRSATGELRPAEGHAR
                     YMRYGGVEYACVVLRDVSEREAAERALHESQERFNAAVNGAEVGIWEWNPQTDRIYRS
                     PLYLQILGHTSAAALPETGSKLLDSFDAESAARVKTIIDDAVRERRKFAFEHLYTRPD
                     GEQRWLRISGAPIFGEDDQLTRIAGSMVDVTDTKKIELQLRADRARQEVIFQRSPLGI
                     MVTRQHDGLVVEANEAVLRMTGYDKENFVGRTTVELGFWGCADDRAQELRDAYTNERS
                     FVRSDGEQIDLKRSARQVEIEGEHYYITTLEDVTERNRREQALRASEARFARLLEVAP
                     LAVAMVEVDSRRVVDINPAFTRVTGYERGMAVGKSIDELDLWTSSGQDEEDIDRVQRM
                     GRIVARDRSIRRQSGEAGYVEYSALLIDMEGKARVLYFMQDITERKRAEIDLARGRER
                     LSRILHASPTAMTISDLDTCAVLDANEAWLNLFGMESSEVIGRSVREMESWGDLSDRA
                     ARLDALRRGESMRDFVTECIRSDGRRFEAICSFEKLELDGRNLLLTTLLDISEQRRVE
                     RELMRRGQLLRQTGAVAKVGGWELDPVAHEMEWTEELYRIYERDPDIGGAQLSDFLAS
                     CCDDSRTSLELALARTVATGEPFDIELPFVTANGNRRWGHIQAMAEVRDGQVSRLYGA
                     FQDITERRQHGEWLRESEARFSRIFDSTPAGIVLFRAEDGRVVDVNPAMCGIGGFGRS
                     DAIGMSIDQLKSLFATDAFDRAAASAHQGVDIWMPARDCWYRRHDGRTGYVSMTASRF
                     EYAGENLVLAFLQDVTERQRAAEEIRRLNETLEQRVRERTAELEAANRELETFSYSVS
                     HDLRAPLRAMTGFSTILMQEHAPKLNPDALELLTRVSSASQRMADLIDALLQLSRVAR
                     QPMKRVPIDFSALAHIVVQELRDTEPQRETQIEIDPDMHVEGDASLLRSVLQNLIGNA
                     WKYSARKPVVKIQMRCRTIEGERVFYVRDEGAGFDMRYAGRLFTAFQRLHSAKDFDGT
                     GIGLATVERIVRRHGGRIWADAEPGKGATFYFTLL"
     gene            12445..13071
                     /gene="ccmA"
                     /locus_tag="VKZ48_00725"
     CDS             12445..13071
                     /gene="ccmA"
                     /locus_tag="VKZ48_00725"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HLU76406.1"
                     /translation="MLQAHRLECIRGERLLFRDLDFALNPGELLRVEGPNGSGKTSLL
                     RMLCGLLSPASGTLQWQGADIRQLGETYRAALTYVGHQNALKADLSGVENLQLGEALA
                     GVTITREAAREALTRLGLAIAADLPTRLLSQGQCRRVALARLMFRARCPLWVLDEPFV
                     ALDTAVVPEIAALLSAHVRTGGMVVYTTHQDVPIEAGERRSLRLGGVQ"
     gene            13068..13796
                     /gene="ccmB"
                     /locus_tag="VKZ48_00730"
     CDS             13068..13796
                     /gene="ccmB"
                     /locus_tag="VKZ48_00730"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018912913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HLU76407.1"
                     /translation="MNRAGIESTVPPSTAAQEASLAAAFVAVVRRDLLLAMRRRADVL
                     TTLVFFVIVVSLFPLGIGPELNTLRTIGPGVLWVAALLAAMLALNRLFAADYADGTLE
                     QMVLTPQPLSVLVLGKVAALWLTTGLPLVVMAPLLGLQYALPAEALYTLVIALALGTP
                     ALGLIGAVGAALTLGARGPGVLVSLLVLPLYVPVLIFGAGAVDAVMSGLDAGGHLSLL
                     GALLLVSLVVAPLASAAALRIALD"
     gene            13799..14521
                     /gene="ccmC"
                     /locus_tag="VKZ48_00735"
     CDS             13799..14521
                     /gene="ccmC"
                     /locus_tag="VKZ48_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HLU76408.1"
                     /translation="MNLYKYAAPSTFYPLAGRLAPWFYGAAAIFTVIGLYIGFFVAPT
                     DFQQGEAYRIIFIHVPAAWMSMIVYLAMAFWAAIGLAFNTRLSSMMALALAPTGAWMT
                     FIALWTGALWGKPMWGTWWVWDARLTSELILLFLYVGFMALHASIDDPRRADRAAAVL
                     AIVGAINVPIIYFSVQWWNTLHQGASVSLTKSPSMATTMLWGMLVMAIAFWMYCIGAV
                     LSRCRAIILERERSADWVKEIA"
     gene            14521..14685
                     /gene="ccmD"
                     /locus_tag="VKZ48_00740"
     CDS             14521..14685
                     /gene="ccmD"
                     /locus_tag="VKZ48_00740"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HLU76409.1"
                     /translation="MEWESWSAFWAMGGYGGYVWGSFGITALVMIGELMLVHRRHRAA
                     REAVKHIHRA"
     gene            complement(14689..15834)
                     /locus_tag="VKZ48_00745"
     CDS             complement(14689..15834)
                     /locus_tag="VKZ48_00745"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HLU76410.1"
                     /translation="MSASTDPTDPQTLQLLLGLYQAIDAEAVWRAGVRLLRHVVPGFH
                     YLMGLPSVGTMPVFIRTTLQIEGDRDVYLARIHAAAPIEAAMHMFPDRRVIRLSDQFP
                     GDSVEQTALYREFMAPEGWRYSFLMPLRDEQGGYVGLFSAIRTTAQGDYTDAEVEVLA
                     ALQPHFERAALRVLRIDEERAARRSLEKSLHRSSLPLASFDTRLRPLYSNRAWARACR
                     DWSGGAGTLPADIAAAVEVLIAQWHQVVLTGEPPRSTAPRTVKHADLPGWQADLQLIV
                     SPGDRLASHPTLVVRIHEPAAKAVRDAASIERILQRLSEAERRVARVAGRGFDNGAIA
                     AALHVSVNTVRTHLRNIYRKLDISNRSQLVALLAPAEAVAEEARDGA"
     gene            16245..19004
                     /locus_tag="VKZ48_00750"
     CDS             16245..19004
                     /locus_tag="VKZ48_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HLU76411.1"
                     /translation="MFGPLGAGISTSNTDRQVNLLRFDLGAGLYSIYVTDYLYVEAGI
                     QNDSGVTQNIYIEPLPGRVRINTGTVTGSVVLTNKHLLEFYNTANAGTARIDNNAGGI
                     LNFEMYSNAATSTITNDAGGTVNFNLGSHAGDATIVNEGALFFNDTASGSAAHITTQG
                     TTGVTIFTDDASAGDSVHDVKDGLLGFNGNATAANAVIDVSGGGPWGLQFGDNATADN
                     AVITIASGLLVVQGNADAGNANITNNDRVLFSGNGSAASATIANRNNLSFFNDSSAGA
                     STITTHNGATTSFRDDSVGGTARLIIEAGGTVDITNHNSMVEVGSIEGAGTINIAGRG
                     FYVGTLNTDTTFSGNVIGAGGGMAKVGTGTLTLTGANTHTAGTLISSGRLIVDGSLSS
                     FVLINGGATLGGSGSVGHVTASANGIVAPGNSIGTLNINGNYVAEAGSIYEVEVNDAG
                     ASDLLNITGTATLNGGTVRVLAEAGTYAPSTTYTILTAGGGVTGNYSNATSNFAFLRP
                     TLSYDPNNVYLTLIRNDATLSSIALTRNQRAVGGTLDGGAGAPPAALAGIYTAITGAS
                     TEQARDYYDQLSGDALTSVPTVALEDANRFSAQLRLRSLGHGVPSALAGRNSPVQLAY
                     GDTNLAQLVSAAGTSGATASPAPGQWGTWVSLSGSRTNADDDGNGPGFDADSTGLQLG
                     LESQLRSGVRVGGALGYTHTRADVDDGRDADSKLDTWHAGLYAGYDTARWFASGSLSY
                     AGHDIDSKRDINVGGASEARANIDAQTLSLWSEFGWHLGTEKVAIDPSISLRLARTHI
                     SGFSEKGSPAALEVDSENVDTQRVGFGVRLASAAQNARLRPSVSLRYERELGDRSASL
                     DARLDGLAGFSVRGTELGRDILSLGVALEANLARNLLLYGSLDAAWRSRGDAQSIQAG
                     LKYVW"
     gene            complement(19014..20243)
                     /gene="ccmI"
                     /locus_tag="VKZ48_00755"
     CDS             complement(19014..20243)
                     /gene="ccmI"
                     /locus_tag="VKZ48_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HLU76412.1"
                     /translation="MTAFYIAAALMVLLAVVWIALPLVRGGRAPQPRRDRANLAILRD
                     QLAELDSDLRAGTLSEEQYEQAKLDLERRVLEETAHQDDTPNVRATPTSGRLAAVAVA
                     LLVSVGAVLLYAQIGSPDALAPQAEQGHQFSPQEIDQMVAQLAERMRANPDDPRGWAI
                     LGRTYYVMQRYQESADAYAELIKRIPPDPDVLTDYADALAMAQGRKLTGKPMEALQQA
                     LALDPDHWKGLALAGTEAFEREDYRAALRYWERLLAQAPPGSEVGQQVAASVAEARKL
                     AGLPPQTDAPATPDTSAPSAAGVSGTVRLAPEIAAKADPTDTVFVYARPVDGPKMPLA
                     IVRMQVKDLPAHFTLDDTQAMTPAARLSQHERVELVARISKSGNASVQSGDLVAGARD
                     VAVGSADLSLVIDTQVP"
     gene            complement(20240..20701)
                     /locus_tag="VKZ48_00760"
     CDS             complement(20240..20701)
                     /locus_tag="VKZ48_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005436523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HLU76413.1"
                     /translation="MRALLALLVWCAAGAAFAQIGQQTEADKRLVDISAELRCLVCQN
                     QTIAESNADLAVDLRRQIREQIADGRSDREIIQYMTDRYGDFVLYRPPFKATTVLLWL
                     GPALLLIVGVLTLRRTLRQRRALMADAPLDEEARRRAEALLADNEEERNDT"
BASE COUNT         3593 a         6967 c         6653 g         3541 t
ORIGIN      
        1 tcatggactc gctcacgcgc tatgccatgg cgcagcgtga aattgcactc gccatcggcg
       61 agccgcccgc caccaagggc tatccgcctt ccgtattcgc caagctgccg caactggtgg
      121 agcgcgcggg caacggcgcg ccgggcggcg ggtcgatcac cgcgttctat accgtgctca
      181 ccgaaggcga cgacctgcag gacccgatcg ccgacagcgc gcgcgccatt ctggacggcc
      241 acatcgtgct gtcgcgcagt ctggccgatc agggccacta tcccgccatc gacatcgagg
      301 cctcggtcag tcgcgtcatg cacgcgctca gttcggaaga ggccatgcag accgtgcgcg
      361 aattcaagtc gctctactcg cgctatcagc gcaaccgcga tctggtgaac gtgggcgcct
      421 atgtacccgg cagcgacccg gtgctcgacc aggcgctggc actctacccg caaatggaag
      481 cttttctgca gcagcgcatg gacgagcgcg cacccatggc cgccggtcag gccgcgctgg
      541 ccaccctgtt cgcgcaagcg cagcccgccc cggccgcctg aggcgcggtg tcgttccctc
      601 caacgccgca gggcgcacca aacctcgaaa tgcgggtgga aaagcgcgca atccgacaca
      661 tggctgtgca ccgcaacgca gtatggttcc gttcatacgc cgcagtgggc cgcgccgcgc
      721 ggctcacccc gcacacgagg agtaagcaag cgtgcagacc gacccgatgc aaaccctgca
      781 ggaactcgcc gaggacgcca cccgcgaggc cctgtgccgt ctgtccgaat ccatgaaaag
      841 ctgccgcgaa agcgaatcgc ggctcgaact gctgcggcgc tatcgcgacg aataccggat
      901 aaagctcgat tcggccacgc gcacgggtgt gtcggcgctg gagttgtcca atttccgcaa
      961 tttcctggcg aagctggatg aagcgatcgc acaacagaat gccgatgtcg cgcactggca
     1021 ggcggcggtg acgcgcagcc gcgccgactg gcaggaagcc gagcgccgcg cacgctccta
     1081 cggcgtgctg aacgagcggc gcgccgtaca ggcgcgcggt cgggaagcgc gcgtggaaca
     1141 gaagcagaac gacgaatacg ccgcgcgggc ggttgcctca ccgcgctggg gttgagcgaa
     1201 gatgagcgca ttgcacatcg tcaccaccgc tatcgccccg gcggcgaccc agagcctctc
     1261 gtcggccgag gaaagcgccg aaacgccttt caccgcgctg ctgggcgaac agatgcaggc
     1321 gcatgcacag ttgcccgcgc aactgctgtc gcaggccgcc gaaaagcagg acaccgacac
     1381 gactgaagcg gaagagttcg aagcgcccga actgacggcc gccgatgcgc aggccctgac
     1441 ggctgcgctg ccggtggagg cgcggcagga cgcgctgccc gcacgcctct tccctgagcg
     1501 cggcaccgaa accttgcgca ccgacgtgac ggcgcgcgac ggccgcaacg accggaccgg
     1561 accgcgcggt cccgccgcag caacgtccgt cccgctcgcc ggcgacgccg cgccggaaac
     1621 cgccggcacc acatcgcgcg ttgcgccggc cttcgaatta cccgctgcgc aagcgatcga
     1681 gacgccgaat ccgaccggtg cggctcaggc gcctgtcacg acacctgcga tcgaacggcc
     1741 cgccgttgcg acgccggtcc acacgctgca acagccggtg ggcagcgcac gctgggccgg
     1801 tgaattgggt cagacggtgc agatcctcat tcgcagcgaa caacaaagcg cgacactgca
     1861 cgtcacgccg gccgacctgg ggccgatcga agtccggatc gacatgtcgg gcgatcaggc
     1921 caacctcagt ttcaccgtgc agcaccccga tacgcgcaac gtgctggaga acgcgctgtc
     1981 gcgactgcgg gacatgctgg ccgaaggcgg tatcgcgttg ggcgatgcac acgtgaatca
     2041 gcaatccggc gaacagcgcg aggcggccgc agaaccggcg catcgcaacg gcggcggaaa
     2101 tgcgctggct cccgacaccg cagcggaaac cattgccgtc cgtgcacgcg tcgggctggt
     2161 ggatacgttt gcctgaaaag gcagtcgaga gtcgagagta aaaacaatca gcagtccgaa
     2221 ctgaattccg ctcgcggcac aaccgcgtga tcggaatcga gggtttttct ctcgaccctc
     2281 cactttccac tctccactgc attactcacg aattgagccg gattccccag cgtattccgc
     2341 tcattggcct tccccgccgc tgccgataat tcgcccatcg atatcttgga tagaccgcgc
     2401 acgcgcagcg cgtttcggat agcacatggc aacacaggct caaccggcag cgcaaacgcc
     2461 gccgacctca ccgactccca ggaaaagccg caagaaactg tggttcacgc tcacgctgct
     2521 gctgctcatc gccggcatgg gcggcgcggg actgatgctg ctcattcagc cgtccacgcc
     2581 gatgagcgaa gaagaagccc tggcggccga agaggccaag cgcaaggcac ggccgccggt
     2641 attcctcgcg ctcgagcctt tcgtggtgaa tctgcgcgat gaggacggcg accactatct
     2701 gcgcctgggc gtcgtgctgg aaggcacgaa ccaggacgcg ctggatgtcg ccaagcagca
     2761 gctgccgcgc atccgcaacg gcatcctgct gctgctgtcg gccaagacca gtgcggagat
     2821 cgccaccatg gaaggcaagc agcgcctcat ggaggaaatc atcgtcgccg cgcgcgcgcc
     2881 gatgacgctg cccgagccga gcaggggcat cgagagcgtg tatttctccg acttcgtcat
     2941 tcaataacgc gacggcgtac aggcgaccat gtccgaattt ctgtcccagg aagaagtcga
     3001 cgccctgctg aaaggcgtct cgggcgagac cgacgaaccg gtcgaggaca cgcctaccga
     3061 aggcccgcgt ccgtacaacc tcgccacgca ggagcgcatc gtccgcgggc gcatgcccac
     3121 gctggaaatc atcaacgagc gcttcgcacg gctgatgcgg ctgggcctgt tcaatttcat
     3181 gcggcgcaac ccggacgttt cggtggggcc ggtgaaagtg cagaagtaca acgagttcct
     3241 ccgcaacctg gtggtgccga ccaacatcaa catcatgcag ttgaagccgc tgcgcggcgc
     3301 ctgcctgatg gtgttcgacc ccagcctcat cttcgcggtg gtggacacca tgttcggcgg
     3361 cgacggccgt ttccacacgc gtgtggaagg ccgcgacttc accgccaccg agcagcgcat
     3421 catccagcgc atgctgaacg tggtggtgga cgactacaag aaatcctggg cgccggtgta
     3481 tcccctgcag atggaatacg tgcgctcgga aatgcacacc cagttcgcca acatcgcgac
     3541 gccgagcgaa gtggtgatca gcacgtcttt cgacgtcgag ttcggctcct ccggcggcag
     3601 tatgcatatc tgcattccct attcggtggt cgagccgatc cgcgaagtgc tctattcgcc
     3661 gacgcagggc gacagctacg agccggacaa gcgatggctg cgcatgctgt cgcgtcaggt
     3721 gcagatcgcc gaagtggaac tggtggccaa tctcgcgcat gtcccgacga cgctcggcag
     3781 cctgctgcag atgaagccgg gcgacgtcat tccgctcgag ttgccgcaga acgtggtggc
     3841 ggaggtcgac ggcgtgccga ttttcgattg caagtacggc acgctcaacg gtcaccacgc
     3901 aatccgcatc gagaaggtcc tcgccgtctc ggcgaaggaa aacaatctgg gagaagacaa
     3961 tggctgattc cgtcgacgac gattggggcg ccgcgctcgc cgaacaggcg gccgcaagcg
     4021 ccgacgcacc ggcggccgcg ccggctgcga aggtgttcga accgctggcc agcagcagcg
     4081 gcgacacggg cacacaggac gggctggcac acgatatcga cctcattctc gatatcccgg
     4141 tgcaactgac ggtggaactc gggcggacca agatcccgat caagaacatc cttcaactcg
     4201 cacagggttc cgtcgtggaa ctggacggtc tggccggcga gccgatggac gtactggtga
     4261 acggctgcct gatcgcgcag ggcgaagtgg tggtcgtgaa cgagaagttc ggcatccgcc
     4321 tgaccgacat catcacgccc tccgaacgca tccgccggtt gagcaagtga tcctgtcgcg
     4381 catcatcgca acactctgcg cgctggcggc aacgcccgcg ctcgcgcaag gcacggccgc
     4441 accggccgcc gaactggcgc ccgcgatgcc gctgggcgcc atgctgcagt cgctgttcgc
     4501 gctgctggtc gtgctcggcg tgatcgtcgg tgccgtctgg ctgctgaagc gcacccagcg
     4561 tctgcagggc gcgggcaacg aagtgcttcg caacgtcgcg gccgtggccg tggggcccaa
     4621 ggagcgcgtg gtggtggtgg aagtcggcga cacctggctg gtgctgggtg tggcgcccgg
     4681 acaggtgcgc tcgctgtaca gcctgccgcg ccagaacctg ccggcgcgcg gcatcaacgc
     4741 accgaaattt tccgactggc ttacgcgcgc acgcggcgga gacgagcgtg cgtaatcgtt
     4801 tcatttcgcc cgcatggctt gccgcagtcg cgctgcttgc gcttgcgccg ctgcctgcgc
     4861 tggcgcagaa cgccggcatc ccggcattca ccagcacgcc gacgcccggc ggcggacagt
     4921 cctacacgct gtccatccag acgctgctcg cgctgagcgc gctgaccttc ctgccggcgg
     4981 tgctgctgct gatgacttca ttcacgcgca tcatcatcgt gctgtcgctg ctgcgccacg
     5041 cgctgggcac gcagacctcg ccacccaacc aggtgctcat cggcctggcg ctgtttttaa
     5101 cgctgttcgt catgggaccg acgctggatc gcgtctacaa cgaggcttat ctgccctaca
     5161 gcgaaagccg gatgtcgttc aacgatgcgc tcgcgcgcgc cgagtcgccg atgaaggatt
     5221 tcatgctgcg tcagacgcgc gaagcggatc tcgcactgtt cctgaaactg gccgatcacc
     5281 cggccgtgga atcgccgcag gaagtgccct ttcgcgtgct cgtacccgcg ttcgtcacca
     5341 gcgaattgaa gacggccttt cagatcggtt ttctggtgtt cattcccttc ctcatcatcg
     5401 acatggtggt ggcctcggtg ctgatgtcga tggggatgat gatgctctcg cccgtgctca
     5461 tctccctgcc cttcaagctg atgctgttcg tgatggtgga cggatggaac ctgctgctcg
     5521 gctcgctgag cgcgagtttc ggataggaga gcttgaaaca tgacaccgca aaccgtactg
     5581 accatcggcc agcaggccct gcagatgacg ctgatgatcg ccggcccgct gctgctcacc
     5641 gcgctgctgg tcggtctggt ggtgtccatc atgcaggcgg ccacgcagat caacgaaatg
     5701 acgctgtcgt tcattcccaa gctggtgggc ctgttcgtcg tgctgatcat cgccggtccg
     5761 tggatgctca ccaccatgac ggaatacatg cgccgcctgt ttcagagcat tccgttcgtg
     5821 atcggctgac gcggtggaga attgaaagtg aagagtcgag agcaaacgcc acgtcgcgcc
     5881 catctctgtc tctccactct ccactctcga ttctcctttc tcgactcagc gtgatctcct
     5941 tcacctccga acaggtcgca ttgtggctcg gcatgtatct gtggccgttc gcccgcgtgc
     6001 tcgccctgtt catgaccgca cccgtgctgc acgaccaggc cgtgcccgca cgggtgaaaa
     6061 tcggtttcag cctgcttgcc gcattgctga tcgcaccgct gctgccggcg acgcagcatg
     6121 tgccgatctc cagcccgcat gccgcactgg tgctcgcgca acaggtgctc atcggcgcgt
     6181 caatcggctt cgccgtgcgt ctggtgttcg ccgcgctgga gatggcgggc gacgtcatcg
     6241 gcctgcagat gggcctgtcc ttcgccggct tcatcagccc gggaacgggc gaacagacgc
     6301 cgatcgtcgg cagttttttc ggcgtgatcg cttcgctggt gttcctggcg atcaacggcc
     6361 atctgatgct gctggctgcg gtgacggaaa gcttttatgc gctgccgatc accaacggcc
     6421 tcgggccgca ggtcaatgtc gcggccatgg cctcggcggg cagcgaggtg ttccgcctcg
     6481 gcctgcatgt ggcgctgccc gtgctcgcca ccatgctgat cctcaacctt gcgctcggcg
     6541 tgcttgcacg cgttgcgccg cagttgaaca ttttcgccat cggcttcccg gcgacgctgc
     6601 tggtgggcat gatcgccctg atgctcgccc tgcccgtgct cggcccgctg ctcgaacaga
     6661 tgctgagcca aagcctgtta ccgccgctgc gctagggcaa cgtgcgaaaa aatgccccgc
     6721 acgcggcggg gcacaaggac ggaacgaaac ctcaatcggc gcgcgaacgg cgcaccaaag
     6781 ccagtccgaa caaccccagc gcaagcaggc cgagcatgcc gggctcgggc accagcaagg
     6841 gctcggtggt gatggcgaag gcgaactgga tagtggtcgc ttcgtcctcg ttggtgatga
     6901 tgcccacgca tccgggcgcc gcaccggccg cttcacactg ctccgcaccc agcgtggcaa
     6961 tgctgcccag catgccgccc gcgtcgaacg ggaagatcga gatcagatag tcgatcccgt
     7021 cgaaagtgaa cggcacgttg aaagctgtgg acgacaccag cacgaaaatg tcggcgcagg
     7081 gttccgccgt attctccgca cagccgggat tgccgggcgg attattgagc gtctcgacga
     7141 acaggaagtc gaacaccaga tcgggcaggc cgggcacggc gatacccccg tcgctctcca
     7201 gcgccagact ggaggtgatc tgtccgccgg tcagcgtggc gaaggtcgag gaaatcgggc
     7261 tgttccagtg cgtgatggtg ttcgacagtc cgatcccggg cagcgtcagc gcgttggtcg
     7321 tgaccgtacc gttcacgacg ccgccgccgg tcagattcgt cgcatcggtg ccgatggtca
     7381 gcgcactgcg gtccccatcc ggcacctcga acgaaccgcc ggtcgtaccc caggacagtt
     7441 cgaaaggtga agtgaacggc gtgccgatgc cgggcgtgta atccgcgtcg gtgaaggcga
     7501 aggaaatact gtagttccac gaggcgacgg gtgcggcctg cgctggcgcc gtaatggcca
     7561 gcgccgccgc aatcgccagt aaacctgccc aattgcgcat gacggctacc tccttgatcg
     7621 tttaagggac cagcggaggc ggctggagcg agcgccgtgc cagacgtcta agacgttcta
     7681 ggcattgaac ttttggcgca atgtcatcga cagtgcgtgg ggaatgtaaa gtgcggcgac
     7741 agggcgttgt agccacgacc gacaggggct ttgaaccgcg tgcgggcggg gtatgccgct
     7801 cagagcaagg tgaagtagaa agtggcaccc ttgccgggct cggcgtcggc ccagatgcgc
     7861 ccgccatgcc ggcgcacgat gcgctccacg gtggccaacc cgatgcccgt accgtcgaag
     7921 tccttcgcgc tatgcagacg ctggaacgcg gtgaacagcc gcccggcgta gcgcatgtcg
     7981 aagccggcgc cctcgtcgcg cacgtagaag acgcgttcgc cctcgatggt gcggcagcgc
     8041 atctggattt tcaccaccgg tttgcgtgca ctgtacttcc atgcgttgcc gatcaggttc
     8101 tgcagtacgc tgcgcagcag cgacgcgtcg ccttccacgt gcatgtccgg atcgatttcg
     8161 atctgcgtct cgcgctgcgg ctcggtatcg cgcagttcct gcacgacgat atgcgccagc
     8221 gcgctgaagt cgatggggac gcgcttcatc ggctgccgcg cgacacgcga caactgcagc
     8281 agcgcatcga tgaggtcggc catgcgctgg ctggccgagg acacgcgcgt caacaactcc
     8341 agcgcatcgg gattgagctt gggcgcatgc tcctgcatga ggatggtgga gaagcctgtc
     8401 atggcgcgca gcggcgcgcg cagatcgtgc gagaccgagt agctgaaagt ttccaattcg
     8461 cggttggccg cctccagttc cgccgtgcgt tcgcgcacgc gctgctccag cgtttcgttg
     8521 agccgccgga tttcctcggc cgcacgctga cgctcggtga cgtcttgcag gaaggcgagc
     8581 accaggtttt cgccggcgta ttcgaagcgc gaagcggtca tcgacacata acccgtgcgg
     8641 ccgtcatggc ggcgatacca gcagtcgcgc gccggcatcc agatgtccac gccctgatgc
     8701 gcggaggcgg ccgcgcggtc gaaagcgtcg gtggcgaaca gcgacttcag ttgatcgatg
     8761 ctcatgccga tcgcatcgga gcgaccgaag ccgccgatgc cgcacatggc cgggttgacg
     8821 tccaccaccc ggccgtcctc cgcgcggaac agcacgatgc ctgccggcgt gctgtcgaaa
     8881 atgcgcgaga agcgcgcctc gctttcgcgc agccactcgc cgtgctgccg acgctcggtg
     8941 atgtcctgga acgcgccgta caggcggctc acctggccgt cgcgcacttc agccatggcc
     9001 tggatgtggc cccagcggcg attgccgttc gcagtgacga acggcagttc gatgtcgaag
     9061 ggctcgccgg tggcgacggt acgcgccaat gcaagttcca gcgacgtgcg cgagtcgtcg
     9121 caacaggagg ccaggaagtc cgacagttgc gcgccgccga tatccggatc gcgttcgtag
     9181 atgcgataga gctcttcggt ccactccatc tcgtgcgcaa cgggatccag ttcccagccg
     9241 ccgaccttgg ccaccgcgcc ggtctgacgc agcaactggc ctcggcgcat cagttcacgc
     9301 tccacccggc gctgttcgct aatatccagg agggtcgtga gcaacagatt gcggccgtcc
     9361 agttccagct tctcgaaaga gcagatggcc tcgaagcgcc ggccgtccga gcggatgcat
     9421 tcggtgacga aatcgcgcat cgattcgcct cgccgcagcg catccagtcg cgcagcgcga
     9481 tcggacaggt cgccccagct ctccatctcg cgcacgctgc ggccgatcac ctcggacgat
     9541 tccatgccga acaggttcag ccaggcttcg ttcgcgtcga gtacggcgca ggtgtccaga
     9601 tcgctgatgg tcatcgcagt ggggcttgcg tgcagaatgc gcgacaggcg ctcgcgtccg
     9661 cgcgccagat cgatctccgc gcgcttgcgc tcggtgatgt cctgcatgaa atacagcacg
     9721 cgcgccttgc cttccatgtc gatcagcaac gcgctgtact cgacgtagcc ggcctcgccc
     9781 gactgccgtc ggatggagcg gtcgcgcgcg acgatgcggc ccatgcgttg cacgcgatcg
     9841 atgtcttcct cgtcctggcc ggaagaggtc cacagatcca gttcgtcgat cgacttgccc
     9901 actgccatgc cgcgttcgta gccggtgacg cgcgtaaacg ccggattgat gtcgaccacg
     9961 cggcggctgt ccacctccac catcgccacc gccaacggcg cgacctccag cagacgcgca
    10021 aagcgcgcct cgctcgcgcg caatgcctgt tcgcgacggt tgcgctcggt gacgtcctcc
    10081 agcgtggtga tgtagtagtg ctcgccctcg atctcgacct gccgggcgga acgcttcagg
    10141 tcgatctgct cgccgtcgga gcgcacaaag gaccgttcgt tcgtgtaggc gtcgcgcagt
    10201 tcctgcgcgc ggtcgtcggc gcagccccag aatccgagct ccaccgtggt gcggccgacg
    10261 aaattctcct tgtcataacc cgtcatgcgc agcacggctt cgttcgcctc caccaccagg
    10321 ccgtcgtgct ggcgcgtgac catgatgccc agcggcgaac gctggaagat gacttcctgc
    10381 cgcgcacggt cggcgcgcag ttgcaattcg atcttcttgg tatcggtgac gtccaccatg
    10441 gagccggcga tgcgcgtcaa ctgatcgtcc tcgccgaaaa tcggcgcgcc gctgatacgc
    10501 agccagcgct gctcgccgtc cggccgtgtg tacagatgct cgaaggcgaa cttgcgccgt
    10561 tcgcgcaccg cgtcgtcaat gatcgtcttc acgcgcgccg ccgattcggc gtcgaaactg
    10621 tcgagcagct tgctcccggt ttccggcaaa gccgcggcgg acgtatggcc gagaatctgc
    10681 agatacagcg gcgagcggta gatgcgatcg gtttgcgggt tccactccca gatgcccact
    10741 tccgcgccgt tgaccgccgc attgaagcgc tcctggcttt cgtgcagcgc gcgctcggcc
    10801 gcctcgcgtt cgctcacgtc gcgcaacacc acgcaggcgt attcgacgcc gccgtacctc
    10861 atgtagcgcg catgaccctc ggccggacgc agttcgccgg tcgcgctgcg gtattccatc
    10921 cggtagacgc gctcgccgac gccgcgcagg gcatcgaagg agtgtgcgaa gcgctccggc
    10981 gtgaccgccg ggaagatgtt gacgaagggc atgccgatca gccgctcggg cagcacgccg
    11041 aaccagcgcg ccgcggccgg attggcgaag gcgaagcggc cgttggcatc cacccaatag
    11101 atggcgtcgc tggtctgatc gagggtgaag cgcgtaatgt cgagttccgc gctgctttgc
    11161 tccacgcgct gcagtttctc cagcagcgcg cgtatcagcc cggcggtgaa cagcaagacc
    11221 agcacgaggc ccaccgcaac cttgagcgcc tgctcgcgcc aggcgcgata gacgtccgcc
    11281 agcgaagagg acaccaccac gcgcaagggg ctgtcgttca cgaggcgcgc ggcctgcagc
    11341 gtctgcccgc cgtgtgcgac ctccagcgcg ccgatctgca tgcgctgcgt gaccgactgc
    11401 tgcggcgcga tgggtgccgc atcgggcggc agccggatca tcagttcgcc gtccgccgta
    11461 tgcacttcca cgcgcgaggc ggacagttcg agtttctggt ggatgtcctc gatcagcttc
    11521 gtggtcagca gcatgccgac cacaccgacg aaagcaccgt cctgacgcac gatccggcgg
    11581 gtgatcggaa tcatcgtctg gccgtaggcg cgcagcggtg tgggcgccat gcgcgatgcc
    11641 acatgctcat gctgcgcatg ccaggccagc agtccttcga acgcggcgac gccgggggcg
    11701 ccctcggctt ggggcacgag atgcacccgg ccgttgcgtt cgatgaggaa gaccgggcgg
    11761 atcgccgtgc cccgcgtatc gagaatattg cggatcaccg cctgatcggc ttcgcccccg
    11821 tcaccggcga gagaccggcc ctccgaggca aagcgggcca gcacctgccc ggaacggtcg
    11881 atctgatagg agagattcgc ctcgacggtt ttggtgagca gcgccgcttc gcgcgcgcgc
    11941 tcggcgatgc ggtcttccag ttcgaggccg atgatccagc cggcgaggat cagcaaggcg
    12001 caggcggtga gcacataacc cacgatgaag cggcgacgca cggcgggtat gctcgtgctc
    12061 gcaggcgaca tcggccgcga gcccgccttc gggcgcgctt caactgcgtt ctggtagatc
    12121 gaactcatac tcccctgcca cgtctgtcgc agcatccgaa tccgtacgca cgacgaccgc
    12181 tctggctggc atccgccctt ttgggtaaaa acctgaagtt cggacaaacg gcgctgcgct
    12241 tctctcgcat cgtccgggac gatgcacggc gcagtcgctc cgccttcggg cggaacgggc
    12301 aacatagccc acctgccttg cacaggcagc acaattctga acgggaagcg ggtaaaagtc
    12361 aggccacgct tcgctttata atgccgctgc ccctcaaccg agccgccgcc gacagacgcg
    12421 cgcgccgaac ccgaaatcct gcacatgctt caagcgcacc ggctggaatg tatacgcggc
    12481 gaacgcctgc tgttccgtga tctggatttc gccctgaacc ccggcgagtt gctgcgcgtg
    12541 gaagggccca acggcagcgg caagaccagc ctgttgcgca tgctgtgcgg cctgctgtcg
    12601 cccgcctcgg gcacgctgca gtggcagggc gccgacatcc gccaactcgg cgaaacctat
    12661 cgcgcggccc tcacttatgt cggccaccag aacgcgctga aagccgatct cagcggcgtc
    12721 gagaacctgc aactgggcga agcgctcgcc ggcgtcacga tcacgcgcga ggcggcgcgc
    12781 gaggcgttga cgcgcctggg cctggccatc gcggcggatt tgcctacgcg tctgctgtct
    12841 cagggccagt gccgccgcgt ggcgctcgcg cgtctgatgt tccgtgcgcg ctgcccgctg
    12901 tgggtgctgg acgaaccctt cgtcgcgctc gacacggcgg tggtgccgga aatcgccgcg
    12961 ctgctgtccg cgcacgtgcg caccggcggc atggtcgtat acaccacgca tcaggacgtt
    13021 cccatcgagg cgggcgaacg ccgctcgctt cggctgggcg gcgtgcaatg aatcgcgccg
    13081 gcatcgagtc caccgtgccc ccatccacgg ctgcgcagga agcgagcctg gccgcggcct
    13141 ttgtcgccgt cgtgcggcgc gatctgctgc tggccatgcg ccgccgcgcc gacgtgctca
    13201 ccacgctggt gttcttcgtc atcgtcgtca gcctttttcc gctgggcatc gggccggaac
    13261 tcaacaccct gcgcaccatc ggccccggcg tgctgtgggt tgcggcgctg ctggccgcaa
    13321 tgctcgcgct gaaccgcctg ttcgccgccg actacgcaga tgggacgctg gagcagatgg
    13381 ttctcacgcc gcaaccgctg tccgtgctgg tgctcggcaa agtcgcggcc ctgtggctca
    13441 ccaccggcct gcccctggtg gtcatggcgc cgctgctcgg cctgcagtac gccctgccgg
    13501 ccgaggcgct gtacacgctg gtcatcgcgc tggcgctcgg cacaccggca ctcggcctga
    13561 tcggcgcggt gggcgcggcg ctgaccctcg gcgcgcgcgg ccccggcgtg ctggtatcgt
    13621 tgctggtgct gccgctctat gtcccggtgc tgattttcgg cgccggcgcg gtcgacgccg
    13681 tcatgtccgg cctcgatgcc ggcggacatc tgtcgctgct cggtgcgctg ttgctggttt
    13741 cattggtcgt cgcgccgctg gctagcgccg cagcgctgcg gattgcactc gattagaaat
    13801 gaatctctac aaatacgccg ctccgagcac cttctatccg ctggccggcc gtctggcgcc
    13861 ctggttctac ggggctgcgg cgatcttcac ggttatcggc ctctatatcg gcttttttgt
    13921 cgcacccacc gatttccagc agggcgaggc ctatcgcatc atcttcatcc acgtgcccgc
    13981 ggcctggatg tcgatgatcg tctatctcgc catggcgttc tgggcggcca tcggcctggc
    14041 gttcaacacg cggctgtcgt cgatgatggc ccttgcgctg gcgcccaccg gcgcatggat
    14101 gactttcatc gcgttgtgga ccggcgcgct gtggggcaaa ccgatgtggg gcacctggtg
    14161 ggtgtgggat gcgcgcctca cttcggaatt gatcctgttg ttcctctacg tcggtttcat
    14221 ggcactgcac gcctccatcg acgatccgcg ccgcgcggat cgcgccgcgg ccgtgctcgc
    14281 catcgtcggc gcgatcaacg tgccgatcat ctatttctcg gtgcaatggt ggaacacgct
    14341 gcatcagggc gcgtcggtca gcctgaccaa gagcccgagc atggctacca ccatgctgtg
    14401 gggcatgctg gtcatggcca tcgcgttctg gatgtattgc atcggtgcgg tgctgtcgcg
    14461 ctgccgcgcc atcattctcg aacgcgagcg cagcgcagac tgggtcaagg agatcgcctg
    14521 atggaatggg aaagctggag cgccttctgg gcgatgggcg gctacggcgg ctatgtgtgg
    14581 ggatcgttcg gcatcaccgc gcttgtcatg atcggcgaac tgatgttggt tcaccgccgt
    14641 catcgcgccg cgcgcgaggc ggtgaagcac attcaccgcg cgtagtcttc acgctccgtc
    14701 gcgcgcttcc tcagcgactg cttccgccgg cgccagcaac gcgaccaact ggctgcggtt
    14761 gctgatatcc agcttgcggt agatattgcg caaatgcgtg cgcacggtgt tcacgctgac
    14821 atgcagcgcg gcggcaatgg cgccgttgtc gaacccgcgc ccggcgacgc gcgcgacgcg
    14881 gcgttccgct tcgctcaagc gctgcaggat acgttcgatc gacgccgcat cgcgcaccgc
    14941 cttggccgcc ggctcatgaa tgcggaccac gagcgtggga tgcgacgcca gccggtcgcc
    15001 gggagagacg atcagttgca gatccgcctg ccagcccggc aagtctgcgt gttttaccgt
    15061 gcgcggtgcg gtggagcgcg gcggctcgcc cgtcagcacc acttgatgcc actgcgcaat
    15121 gagaacctcc accgccgccg cgatgtccgc cggcaaggtc ccggcgccgc ctgaccagtc
    15181 tctgcacgcc cgcgcccagg cacgattgct gtacagcggc cgcagccgcg tgtcgaagga
    15241 ggcgagcggg agcgaggacc ggtgcagcga tttctccaga gaacggcgcg ccgcccgctc
    15301 ttcgtcgata cgcaggacgc gtaaggccgc gcgttcgaaa tgcggctgca acgcggccag
    15361 cacttcgact tcggcgtccg tgtaatcgcc ctgcgcagtc gttcggatcg cgctgaacaa
    15421 tccgacatag ccgccctgct catcgcgcaa gggcatgagg aaggaataac gccagccttc
    15481 cggcgccatg aattcgcggt acagggctgt ctgctcgacg ctgtcccccg ggaactggtc
    15541 cgacagccgg atcacccggc gatccggaaa catgtgcatg gccgcctcga tcggcgcagc
    15601 agcgtggata cgcgcgaggt agacgtcgcg atcgccttcg atctgcagcg tggttcgaat
    15661 gaacaccggc atggtgccga cggaaggcaa gcccatgagg tagtgaaagc ccggcacaac
    15721 gtgccgcagc aaccgcacgc cggcgcgcca gactgcctcc gcgtcgatgg cttgatagag
    15781 ccccaacaac aattgcaggg tttgcgggtc cgtcggatcc gtggaggccg acaacggtaa
    15841 cgtatcgtcc ggtgctgcag ttgcgggcaa ggaggggtcc ggcgccacga cagtcatccg
    15901 atcaaaaatc atcggatcgg acgatatctc aaagtcccgc atgacgtcat catccccccg
    15961 ccaacgcggg ccgcctgtcc ggtcccgacg gccgcgcgtt gcacttcgca tcgaataagc
    16021 aagaacaagg aatcgtcatg cgccgcaccc gctccccctc acgtcgtccg ttccgtgtca
    16081 aacccctcgt acacctgtgt cagttggcgc tcgctgcctg cagcaccttt gtcgccccgc
    16141 aggcgaccgg cgcagatcga tcgtggattt caaccgtcgg cggcgactgg agcgacgtcg
    16201 cgaactggtc ggggggcgca gcgccaacca ccggcgacgt cgcagtgttc ggccccctgg
    16261 gtgccggcat ctcgaccagc aacacggatc ggcaagtcaa tctgctgcgg ttcgatctcg
    16321 gcgcaggcct gtactccata tacgtgaccg actacctgta tgtggaagcg ggcatacaga
    16381 acgactcggg tgtcacgcag aacatctaca tcgagccgct gcccggtcgg gtgcgcatca
    16441 acaccggtac cgtaacaggc agtgtggtcc tcaccaacaa gcacctgctg gagttctaca
    16501 acacagcgaa cgccggtact gcgcgcatcg acaacaacgc cggcggcata ttgaacttcg
    16561 agatgtactc caacgccgcc acctcgacga tcaccaacga cgccggtggc accgtcaact
    16621 tcaacctcgg ctcgcacgcc ggcgatgcca cgatcgtcaa cgaaggcgcg ctgttcttca
    16681 acgacacggc gagcggcagc gccgcgcaca tcacgacgca aggcacgacc ggcgtgacga
    16741 tctttaccga cgacgcctcg gccggcgatt ccgttcacga cgtcaaagac ggactcttgg
    16801 gtttcaacgg caatgccacc gcggccaacg cggtgatcga cgtctcgggc ggcggcccat
    16861 ggggtctcca gttcggcgac aatgcgacgg ccgacaacgc cgtcatcacc atcgcgagcg
    16921 gcttactcgt cgtgcagggc aacgccgacg cgggcaacgc gaacatcacc aataacgacc
    16981 gggtcctgtt cagcggcaac ggctccgcgg caagtgcgac catcgcaaac cgcaacaacc
    17041 tgagcttctt caacgactcg agcgccggcg catcgaccat cacgacgcat aacggcgcaa
    17101 cgacgtcgtt ccgcgacgat tccgtcggcg gcactgcacg cctgatcatc gaggcgggcg
    17161 gcacggtgga catcaccaac cacaacagca tggtggaagt cggttccatc gaaggcgccg
    17221 gcacgatcaa catcgccgga cgcggcttct acgtcggcac actcaatacc gacaccacct
    17281 tttcgggcaa cgttatcggc gccggcggcg gcatggccaa agtcggcacc ggcacgctga
    17341 cactcaccgg cgccaacact catactgcgg ggacactgat ctcgagcggc cgcctgatcg
    17401 tcgacgggtc gctctcttcc ttcgtgctca tcaatggcgg cgccaccctc ggcggcagcg
    17461 gcagcgtggg tcacgtcacg gccagcgcca acggcatcgt cgcacccggc aattcgatcg
    17521 gcacgctgaa tatcaacggc aactacgtcg cggaagccgg ttcgatctat gaagtggaag
    17581 tgaacgacgc cggcgcgagc gacctgctga acatcaccgg cacggccacg ctgaacggcg
    17641 gcaccgtgcg cgtgctggcc gaggcaggga cgtacgcacc cagcaccacc tacaccattc
    17701 tcaccgccgg aggcggcgta accggcaact acagcaacgc gacgtcgaac ttcgcctttc
    17761 tccggccgac gctcagctac gaccccaaca acgtctatct gacgctgatc cgcaacgacg
    17821 ccacgctgag cagcatcgcc ctgacgcgca atcagcgcgc cgtcggcggc acgctggatg
    17881 gcggcgcagg cgcaccgcct gcggcactgg cgggcatcta cacggcgatc accggcgcaa
    17941 gcaccgagca ggcgcgcgac tactacgacc aactctccgg cgatgcgctc accagtgttc
    18001 cgactgttgc gctggaggac gcgaatcgct tcagcgcgca gttgcggctg cgttccctcg
    18061 gtcacggcgt gccctctgcg ctggccggca ggaacagccc cgtgcaactc gcttacggcg
    18121 acaccaacct cgcgcaactg gtcagcgctg ccggtacgag cggcgccacg gcctcgcccg
    18181 caccgggaca atggggcacg tgggtatcgc tgagcggctc gcgtaccaat gcggacgacg
    18241 acggcaacgg ccccggcttc gatgccgata gcacagggct gcaactgggt ttggaatcgc
    18301 agttgcgttc gggcgtgcgt gtcggcggcg cgttgggcta cacgcatacg cgcgccgacg
    18361 tggacgacgg ccgcgacgcc gacagcaagc tggatacctg gcatgcgggt ctctacgccg
    18421 gttacgacac cgcgcgctgg ttcgcttccg gttcgctcag ctatgccggc cacgacatcg
    18481 acagcaaacg cgacatcaat gtgggcggcg caagcgaagc gcgagcgaac atcgatgcgc
    18541 agaccctctc gctgtggagc gagttcggtt ggcatctggg cacggagaaa gtcgccatcg
    18601 atcccagtat cagcctgcgt cttgcgcgca cgcacatttc cggcttcagc gaaaaaggtt
    18661 cgcccgcggc gctggaagtc gacagcgaga acgtcgacac ccagcgcgtc ggtttcggcg
    18721 tgcggctcgc gagcgcggcg cagaacgcac gtctgcggcc gagcgtctcg ctacgttacg
    18781 aacgcgagtt gggcgaccgc tcggcctcgc tcgatgcgcg tctggacggc ttggccggct
    18841 tcagcgtgcg cggcacggaa ctgggacggg acatcctgag cctcggcgtg gcgttggaag
    18901 cgaacctcgc gcgcaacctc ttgctctacg gcagtctcga cgccgcatgg cgcagccgcg
    18961 gcgacgcgca atcgatacag gcgggattga agtacgtctg gtagcgaggt cgctcaaggc
    19021 acctgcgtat cgatgaccag cgacaggtcg gcactgccga ccgccacatc gcgcgcaccg
    19081 gcgacgagat cgccgctttg cacgctggcg ttgcccgatt tggagatgcg cgcgacaagt
    19141 tccacgcgct cgtgctgact cagccgcgca gcgggtgtca tggcctgcgt gtcgtcgagc
    19201 gtgaagtgcg ccggcagatc cttgacctgc atgcgcacga tggcgagcgg catcttcggc
    19261 ccatcgacgg ggcgcgcata gacgaagacc gtgtctgtcg gatcggcctt cgcggcaatt
    19321 tccggcgcaa ggcgcaccgt gccgctcacg cccgcggccg atggagcgga tgtatcgggc
    19381 gtcgccggcg catcggtctg cggcggcaga cccgccagct tgcgcgcttc cgcaacgctc
    19441 gctgccacct gctgcccgac ttccgagccg ggcggtgcct gcgcaagcag tcgctcccag
    19501 tagcgcagcg cagcgcgata gtcctcgcgc tcgaatgctt cggtgccggc aagcgccagg
    19561 cccttccagt ggtccgggtc gagtgcgagc gcctgttgta gcgcctccat cggtttgccc
    19621 gtgagcttgc ggccctgcgc catggcgagt gcatcggcgt agtcggtcag cacatccgga
    19681 tcgggcggaa tgcgtttgat cagttcggca taggcatcgg ccgactcctg gtagcgctgc
    19741 atgacgtaat aggtgcgccc gaggatggcc cagccgcgcg gatcgtcggg attggcacgc
    19801 atgcgctcgg ccagttgcgc caccatctgg tcgatttcct gcggcgagaa ctgatgcccc
    19861 tgctccgcct gcggcgccag cgcgtcgggg ctgccgatct gcgcataaag cagcacggcg
    19921 ccgaccgaga cgagcaacgc cacggcgacc gccgccaggc ggccggatgt gggcgtcgct
    19981 cgaacgttcg gtgtgtcgtc ctggtgcgcc gtctcttcca gcacccggcg ctccagatcg
    20041 agcttcgcct gttcgtactg ttcttcggac agcgtgccgg cgcgcaggtc gctgtccagt
    20101 tccgcaagct gatcgcgcag gatggcgaga ttggcgcggt cgcgtcgcgg ttgcggtgcg
    20161 cgaccgccgc gcaccagcgg cagcgcgatc cacaccacgg caagcagcac catcaacgcg
    20221 gcggcgatat aaaacgcggt cacgtgtcgt tccgttcttc ttcgttatcg gcgagcagcg
    20281 cttcggcgcg gcggcgcgct tcctcatcga gcggcgcgtc ggccatcagc gcacgacgct
    20341 ggcgcagcgt gcggcgcagg gtgaggacac cgacgatcaa caacagcgcg ggaccgagcc
    20401 agagcagcac cgtggtcgcc ttgaacggcg ggcggtagag cacgaagtcg ccgtagcgat
    20461 cggtcatgta ttggatgatt tcgcggtcgc tgcggccgtc ggcgatctgc tcgcgaatct
    20521 gccggcgcag atcgacggcc agatcggcgt tcgactcggc gatcgtctgg ttctggcaga
    20581 ccaggcagcg cagttcggcg gagatgtcca ccagacgctt gtccgcctcg gtctgttggc
    20641 cgatctgtgc gaatgccgcg ccggccgcgc accacacgag caaggcgagc agggcccgca
    20701 tcagcgcgac agcctttcga ccagcggcag gatcttttcc ttcagcgcgt cttc
//
LOCUS       DATGOU010000011         4114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3134773, whole genome shotgun sequence.
ACCESSION   DATGOU010000011 DATGOU010000000
VERSION     DATGOU010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3134773"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /gene="miaA"
                     /locus_tag="VKZ48_00765"
     CDS             <1..833
                     /gene="miaA"
                     /locus_tag="VKZ48_00765"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011544648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HLU76414.1"
                     /translation="AQVYRDMDVGTAKPTPEERRTVPHHLIDLIDPTEAYSAAQFRDD
                     ALGLMQAIHARGRTPLLVGGTMLYFKALREGLSDLPRADAQVRAALDAEAAARGWPAL
                     HAELARIDPVTAARLPPNDSQRIQRALEIHRITGQPMSSLIAACAANAALPFRLIEMA
                     LVPHERAALHARIAERFDAMLAAGLVDELRGLRERYTLHAGLPSMRCVGYRQAWSFLE
                     GEYDARELHERGIYATRQLAKRQLTWLRGMSDVAFFDCFDDRLDAHVLKHIERALHAA
                     "
     gene            1068..1505
                     /gene="gspG"
                     /locus_tag="VKZ48_00770"
     CDS             1068..1505
                     /gene="gspG"
                     /locus_tag="VKZ48_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007736963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HLU76415.1"
                     /translation="MRYKEFHARRGCRGFTLLELLVVMVIIGLLAGYVAPRYFSQLSK
                     SEVRTAQAQINALGKALDHYRLDVGHYPSSEQGLAVLVTRPENEPKWSGPYLEKAVPL
                     DPWGKPYQYRFPGQHGEYDLYSFGKDGQPGGTEHAADVTSWGS"
     gene            1599..2777
                     /locus_tag="VKZ48_00775"
     CDS             1599..2777
                     /locus_tag="VKZ48_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HLU76416.1"
                     /translation="MQFEIRGLKGAQGLISVSVNAIDEASALAMAQAQGLAVLSIRSR
                     RGFALPSIRKPFPLLLFAQELLALLTAGLSLIEVVETMIEKEQRPDTRKLLVDIRSAL
                     FEGRSFSQALEAHPSTFPTLFVATVRAAERTGDLSEALMRYIDYHQRIDQVRKKIVSA
                     SIYPAVLIAVGGLVTLFLLGYVVPRFAGIYADSGQSLPFLSRLLIDWGQLLNQHGTAA
                     LLMALLLIGAAVYGFLRAPAVLGRLARRIPSIGSRMRVYQIARFYRTLGMLLRGGTPL
                     VPALAMVAGLLPAPMRASLAAATQRVREGASISVAMESEDLTTPVALRMLRVGEKSGD
                     MAEMMERIAVFHDEELARWIDWFTKLFEPVLMALIGVVIGAIVVLMYLPIFELAGSLQ
                     "
     gene            2774..>4114
                     /locus_tag="VKZ48_00780"
     CDS             2774..>4114
                     /locus_tag="VKZ48_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HLU76417.1"
                     /translation="MNMAEDVLVAFTAEDIDAARKLAASRASTITAALDELSGLPADV
                     FTGRLGVTMHYPVVSMEDLHRWTPAFDLLPFPEALRAQMLLFRNDADRLCLVLGDPFA
                     TDQIAWAEERIAQNFDWVLAHHADVAAYLARQEESLSAVEAILPAAMAVTADASGVEN
                     LSFLSITEDTSQVVRLVRSTLYDALKTEASDIHLETGPTGLGIKYRIDGVLVYIGSLP
                     GLELAEQVISRVKVMAELDISERRVPQDGRFKVSVRGREIDFRVSIMPSIFGEDAVLR
                     ILDKQQLADEASGLTLDSIGFDPDARTIVRQLASEPYGMLLITGPTGSGKTTTLYAAI
                     SEINQGRDKIITIEDPVEYQLPGVLQIPVNEKKGLTFARGLRSILRHDPDRVMVGEIR
                     DPETAQIAIQAALTGHLVFTTVHANNVFDVLGRFTHMGVDTYSFAAALNGVVAQR"
BASE COUNT          756 a         1356 c         1252 g          750 t
ORIGIN      
        1 cggcgcaggt ttatcgcgac atggacgtcg gcactgcgaa gcccacgcca gaggagcggc
       61 gcaccgtgcc gcatcacctc atcgatctca tcgatccgac cgaggcctac tccgccgcgc
      121 agttccgcga cgacgcgctg ggtctcatgc aggcgatcca cgcgcgcgga cgcacgccgc
      181 tgctggtggg cggcacgatg ctttatttca aggcgctgcg cgaagggctg tccgatctgc
      241 cacgggccga tgcacaagtg cgcgccgcac tggatgccga agcggctgcg cgcggctggc
      301 ccgccctgca cgccgaactg gcgcgcatcg atccggtcac tgccgcgcgc ctgccgccga
      361 acgattccca gcgcatccag cgcgcgctgg aaattcaccg catcaccggc cagccgatgt
      421 cttctctcat cgcggcatgc gcggcaaatg cggcgctgcc tttccgattg atcgaaatgg
      481 cgctggtgcc gcatgaacgg gccgcgctgc atgcgcgcat cgccgagcgc ttcgacgcca
      541 tgctcgccgc cggcctggtg gacgaattgc ggggcttgcg cgagcgctac acgctgcatg
      601 cgggtctgcc gtccatgcgc tgcgtcggct atcggcaggc atggtccttc ctcgaaggcg
      661 aatacgacgc gcgcgaactg cacgagcgcg gcatctacgc cacgcgtcaa ctcgccaagc
      721 gccaactcac ctggctgcgc ggcatgtccg acgtcgcctt cttcgactgc ttcgacgatc
      781 ggctggacgc gcacgtgctg aagcacatcg agcgcgcgct gcacgccgcc tgaaccctcc
      841 gccgcgccac accggtgcgg gtcgaaaaat tgctcctcgg ccgtaaaacg gtcgcacccg
      901 cgcattggcc ttcggtacaa cgcatccgcg cggcgcaaag acgcgccgca cgcgtcttcg
      961 aggcgatacg ggcgctgtca aaaatcgtgt cgccgcgcgt gcgacgcagg gcgacacacc
     1021 ataatcgaca acgattgcag gtccacgcga tcctttcagg aggagcagtg cggtacaagg
     1081 agtttcacgc gcggcggggc tgtcgcggtt ttacgctgct cgagctgctg gtggtaatgg
     1141 taatcatcgg cctcctggca ggctacgtcg cgccgcgtta cttctcgcaa ctgagcaagt
     1201 ccgaagtgcg cacggcacaa gcgcagatca acgcgctcgg caaggcgctc gatcactacc
     1261 gcctcgatgt gggtcactac ccttcctccg aacaaggact cgcggtgctt gtcacgcgcc
     1321 ccgagaacga accgaagtgg agcggcccgt atctggaaaa ggcggttccg ctcgatcctt
     1381 ggggcaagcc gtaccagtac cgcttccctg ggcagcatgg cgagtacgac ctatattcat
     1441 ttggaaagga tgggcaaccg ggcggcaccg aacacgccgc cgacgttacg agttggggct
     1501 cgtaacaccg cgcgaataac agaacccgtt ctcggcggtg cttcgacgac acgcacgacc
     1561 ggtgcttgta tttcaagcgc tgcgggggag aggccgctat gcagtttgaa atccgggggc
     1621 tgaaaggtgc gcaaggactg atcagcgttt cggtcaatgc aatcgacgaa gcctccgcac
     1681 tggccatggc acaggcgcaa ggcttggccg tgctgtcgat acgcagcaga cggggttttg
     1741 cgcttccgtc gatccgcaag ccgtttccat tgctgctgtt cgcgcaggaa cttctcgccc
     1801 tgctcacggc cggcctgtcg ctcattgaag tcgtcgagac catgatcgaa aaggagcagc
     1861 ggcccgacac gcgtaagctg cttgtcgata tccgcagcgc actgttcgaa ggccgctcct
     1921 tctcccaggc gctggaagcg catccgtcaa cctttccgac actgttcgtt gccaccgtac
     1981 gcgcggcaga gcgcacgggc gatctgtccg aagcattgat gcgctacatc gattatcacc
     2041 agcgcatcga ccaggtgcgc aagaagatcg tcagcgcatc catctacccg gcagtgctga
     2101 tcgccgtcgg cggcctcgtg accttgtttc tgctcggcta cgtcgtaccg cgcttcgccg
     2161 gcatctatgc ggactcgggg caatcactgc ctttcctgtc gcgcctgctg atcgattggg
     2221 gccagttgct gaaccagcac gggaccgccg cactgctgat ggccttactg ctgatcggcg
     2281 cggcggtata cggattcctg cgggcacccg ctgtactcgg ccgcctcgcc cggcggattc
     2341 ccagcatcgg cagccggatg cgcgtctatc agatcgcccg cttctatcgc acgctcggca
     2401 tgttgttgcg cggcggcacg ccgctggtgc cggcactcgc catggtggcc ggattgttgc
     2461 ctgccccgat gcgcgcttcg ttggccgccg ccacccagcg cgtgcgtgaa ggcgcaagta
     2521 tctcggtggc catggaatcg gaggatctca ccacaccggt ggcattgcgc atgcttcggg
     2581 tgggcgagaa aagcggcgac atggcagaaa tgatggagcg tatcgccgtc ttccacgacg
     2641 aggaactcgc acgttggatc gattggttca ccaagctatt cgagccggtg ttgatggcgc
     2701 tcatcggcgt ggtcatcggc gccatcgtgg tgcttatgta tctgccgatt ttcgaattgg
     2761 cggggagtct gcaatgaaca tggccgaaga cgtcctcgtc gccttcaccg cggaagacat
     2821 cgatgcagca cgcaagctcg cagccagccg cgcttcgacc atcactgcag cactcgacga
     2881 actgagcgga ctgccggccg acgtatttac cggccggcta ggcgtcacca tgcactaccc
     2941 ggtggtcagc atggaagacc ttcatcgctg gacacccgcg ttcgacctgc tgcccttccc
     3001 cgaagcattg cgcgcgcaaa tgctgctgtt ccgcaacgac gcggatcgac tgtgcctcgt
     3061 actgggagat ccgtttgcca cagatcagat cgcctgggcc gaagaacgca tcgcccagaa
     3121 cttcgactgg gtgctcgcgc accatgccga cgtggcggcc tatcttgccc ggcaggagga
     3181 atcgctgtcc gccgttgaag cgatcctgcc ggccgcaatg gccgtaactg ccgatgcgtc
     3241 cggcgtcgaa aatctttcat tcctcagcat cacagaagac acgagccagg tcgtacgact
     3301 ggtgcgctcc actttgtacg acgcattgaa aaccgaagcg tcggacattc acctggaaac
     3361 cgggccaacg ggactcggca tcaaatatcg catcgatggc gtattggtct atatcggcag
     3421 ccttccgggg ctggagctcg ccgaacaggt aatctcgcgc gtgaaggtca tggccgaact
     3481 cgacatctcc gagcggcgcg tgccgcagga cggtcgcttc aaggtcagcg tacgcggcag
     3541 ggagatcgac ttccgcgtct cgatcatgcc gtccatcttc ggcgaggacg cagtgttgcg
     3601 cattctggac aagcagcaac tcgccgacga ggcgagcggt cttacgctcg atagcatcgg
     3661 ctttgatccg gatgcgcgca ccatcgtgcg gcaactcgct tccgagccct acggcatgct
     3721 gctcatcact ggtccgaccg gcagcggcaa gacgacgacg ctgtacgcgg cgatatcgga
     3781 gatcaaccag ggccgcgaca agatcatcac catcgaagac ccggtcgaat accaactgcc
     3841 cggcgtgctg caaattcccg tgaacgaaaa gaaaggcctc actttcgcgc gcggtctgcg
     3901 ttcgatcctg cgtcacgatc cggaccgcgt catggtgggg gagatccgcg accccgaaac
     3961 cgcacagatc gcaatccagg ccgccctcac cggtcacctc gtcttcacca ccgtgcacgc
     4021 gaacaacgtc ttcgatgtgc tcggccgctt cacccacatg ggcgtggaca cctacagctt
     4081 cgccgcagcg ctgaacggcg tggtcgccca acgc
//
LOCUS       DATGOU010000012         6981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_29290, whole genome shotgun sequence.
ACCESSION   DATGOU010000012 DATGOU010000000
VERSION     DATGOU010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29290"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1977)
                     /locus_tag="VKZ48_00785"
     CDS             complement(<1..1977)
                     /locus_tag="VKZ48_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLU76418.1"
                     /translation="MPGRSPAPLSLFALAVFAASTVEAQDALDEIVVSASRHEQRAFD
                     APAAVNSVGADEIRGAGPRLNISEPLRRLPGIVALERQNYAQDLQLSVRGFGARSAFG
                     VRGLRILVDGIPATMPDGQGQVSAIDLGSAERIEVLRGPLAQLYGNAAGGVVQVTTAV
                     GGADRIEGSIDVARDGTHRLGLRMLGERGAVRGAVSASDFRTDGWRRHSAAERQLYNA
                     KLQFDIAPATRLTLVGNVFEQPEAEDPGGLTRADFRNAPRSVAPIAIEQDTRKTVRQH
                     QAGLTLEHTIDSTRTLTARLYFGQRDLFQALAIPPAAQAGPKSSGGIVDLDRDYGGLG
                     LQYTHRTGAVEYTLGFEADGLREHRRGYVNDGGRRGALKRDEDDTVWNADVYAQLSWR
                     FAERWSALAGVRRSMVRFDVDDDFITGGNPDDSGDVKYHATSPVTGLTFHASDALNLY
                     VNFGLGFETPSFTELAYTADDSGLNFALDPARSRHWEAGMKYRPSNKTQLDVAAFRIE
                     TRDELVVASNVGGRTVYRNADRTERHGLELLYRMRLSDTVAAHLAWTELVARFDSDDV
                     TDGNRLPGTPDRQLYAALLWQPQWQGALEGFGAALEAVHVGRMYVDDANSDAADAHTR
                     LNLRAEVFKDYGDWSLRSYARLDNLTGRDYAGSVI"
     gene            complement(2027..3163)
                     /locus_tag="VKZ48_00790"
     CDS             complement(2027..3163)
                     /locus_tag="VKZ48_00790"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLU76419.1"
                     /translation="MSVVNQMLRDLDRRQAGEQALYAQHVQPPASTRRGLWLACALAF
                     MLVLAASYGALRYFDRPASVDSHVRDIQAPVAAIDTPRNDEAEAPSPSAPVVESVVVP
                     TAPEEAPAVSQQIAAVETPATETPAAPQVQPEPSRPVVEPAPVLSTVPVDPAPTDVVA
                     NVERPAQTPQAAPAERDAGPTKIEVRPHTPADTADSEYRRAAALINRGRNEEARTALT
                     QALELDPAHEAARQTLAVLLIDARANADAERLLAEGLRINPAQTNFAIVLARLHLDRG
                     DDHGALQVLRAHADAAQANAEYGAFTAALLQRLDRHAEAIEAYRDVLKFAPNAGIWWV
                     GLGRSYEATQQPAEAADAYAHARDTGTLRPAVAQFVEQKLQTLR"
     gene            complement(3160..4062)
                     /locus_tag="VKZ48_00795"
     CDS             complement(3160..4062)
                     /locus_tag="VKZ48_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLU76420.1"
                     /translation="MIYLAHFGLREAPFSITPDTSFFCSCRGMQGALNTLMFAVRNGE
                     GFIKITGEVGTGKTLLCRKFLGSLGEDWIAAYIPNPNISPHTLLRAVASELGAELPAG
                     DDEHSLMRAIDTHLLALAQAGKRPVLCVDEAQAMPIETLEMVRLLTNLETEKRKLLQV
                     VLFGQPELDEKLARQEIRQLRQRITFEHRLTPLSLDETAYYLAHRLVVAGYAGGLLFS
                     RGATRVIHRASRGVPRLANILAHKSLMLAYGAGAHQIRRADALRAVSDTPAARQPTRV
                     LWWLGLLGAATLGAAAGFVMIGRL"
     gene            complement(4062..5822)
                     /gene="mshL"
                     /locus_tag="VKZ48_00800"
     CDS             complement(4062..5822)
                     /gene="mshL"
                     /locus_tag="VKZ48_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus (MSHA type) biogenesis protein MshL"
                     /protein_id="HLU76421.1"
                     /translation="MKKTLILALACALTACAVPRDPQTLDRSIVDEINAAAAAKPQPA
                     RDAAIDQALIPPLRLELPKVDDRELEPRFDVSVNDAPAQQVLLAIVSGTRYSMLVHPD
                     VAGTITVDLKDTTVPEALEALRELYGYEYRIDGNRIIVQAASLQTRVFRVNYLAGQRL
                     GRSELRVISGSVADAGTASPGAAAAAAAIPTAVGGQPGTQGSTSRVTDSARVLTSTST
                     DFWRDLEQTLKVIVGTEPGRNVVVNPQAGVIVVRGMPADMRNVQDYLRAIKLSVERQV
                     MIEAKIIEVTLSDAYQAGINWAVFGKGKFAGGVMGPGAQLSNVGEISARNRGIFASPE
                     NPVREIGTGAVSSVTEAAGLPGGLFGLAFQTHNFAAVLEFLEGQGSVQVLSSPRIAAL
                     NNQKAVLKVGTDEFFITNVQSGNTTSGVIGGNTTSFPTLTLQPFFSGVSLDITPQIDD
                     ASNVILHVHPSVSNVVQDNRQINLGDLFGENIVLPLARSTVSETDSVVKVADGNIVAI
                     GGLMKVDISDSKSGLPGLQNVPVLGNAFGSRDRVAVKKELVILIKPTIIGGETNSDDI
                     GGARDRLLSLGARRGGAVAD"
     gene            complement(5819..6190)
                     /locus_tag="VKZ48_00805"
     CDS             complement(5819..6190)
                     /locus_tag="VKZ48_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76422.1"
                     /translation="MKLVRLYAFAALALGTMAAYAQALNDPMRPPQFSVTASAPEAGP
                     VLQSVIISKERRYAIISGERIAVGGVYGEAKLVRIAENEVTLRDAAGETVLKLVPEIK
                     KQIVAPKTVSHDALSGRKTKQ"
     gene            complement(6187..6864)
                     /locus_tag="VKZ48_00810"
     CDS             complement(6187..6864)
                     /locus_tag="VKZ48_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76423.1"
                     /translation="MKQWWLKWSERIDAMVLRERAIVFFAAAMVMIFLFNTLLSGPVT
                     KRQREVARQLAQQQLDTRKLQDQIQILVAQSTRDPDVERRKQIDDLKQRIAELDTRLA
                     EKQRELVAPDHVPALLEEMLRRERRLELVGLQSLPPAPLFDASAHAGDRQDGTRVSAL
                     QVYRHGVELTVRGSYFDLLRYLDELERLPLRLYWGEVEIAGDEYPQISMKLSVYTLSL
                     ERTWVVV"
BASE COUNT         1125 a         2213 c         2373 g         1270 t
ORIGIN      
        1 gatgaccgag ccggcgtaat cccgacccgt caggttgtcc aggcgcgcgt agctgcgtaa
       61 ggaccaatcg ccgtagtcct tgaacacctc cgcccgcaaa ttgaggcgcg tgtgcgcatc
      121 ggccgcgtcg ctgttggcgt cgtccacata catgcgtccg acgtgtacgg cttccagcgc
      181 ggcaccgaag ccctccaacg cgccctgcca ttgcggctgc cacagcagcg ctgcatagag
      241 ttgccggtcc ggcgtgccgg gcaggcggtt gccgtcggtt acgtcgtcgc tgtcgaaacg
      301 cgccaccagc tccgtccagg caagatgtgc ggccaccgtg tcgctcagcc gcattcggta
      361 gagcagttcg aggccgtgac gctcggtgcg gtcggcgttg cgatagaccg tgcggccgcc
      421 gacgttcgag gcgaccacca gctcgtcgcg cgtctcgatg cggaatgcgg cgacatcgag
      481 ttgcgttttg ttcgacggcc gatatttcat gccggcttcc cagtgccgcg aacgggccgg
      541 atcgagcgcg aagttgagtc ccgaatcgtc ggcggtatag gccagttcgg taaagctcgg
      601 cgtctcgaaa cccagaccga agttgacgta gagattgagc gcgtcgctcg catgaaaagt
      661 cagaccggta acgggactgg tggcgtggta cttcacatcg ccgctgtcat cggggttgcc
      721 gccggtgatg aaatcgtcgt ccacgtcgaa gcgaaccatg ctgcgccgca cgccggcgag
      781 cgcgctccag cgctcggcga aacgccatga aagctgcgca tagacatccg cgttccacac
      841 cgtgtcgtcc tcgtcgcgct tgagtgcgcc gcggcggccg ccgtcattga cgtaaccgcg
      901 ccgatgttcg cgcaatccgt ccgcttcgaa gccgagggtg tattcgaccg cgccggtgcg
      961 atgcgtgtac tgtagaccaa ggccgccgta atcgcgatcc agatcgacga tgccacccga
     1021 tgatttcggg ccggcctgcg ccgcaggcgg aatcgccagc gcctgaaaca gatcgcgctg
     1081 tccgaagtaa aggcgcgcgg tcagtgtgcg cgtcgagtcg atagtgtgtt cgagcgtgag
     1141 gccggcttgg tgctgcctaa cggttttgcg cgtgtcctgc tcgatggcaa tgggtgcgac
     1201 gctgcgcggc gcatttctga agtctgcccg cgtcaggccg ccgggatctt cggcttccgg
     1261 ctgctcgaac acattgccca ccagtgtcag acgcgtggcc ggcgcgatat cgaactgcag
     1321 tttggcgtta tagagttgcc gttcggccgc gctgtgccgc cgccagccgt cggtacggaa
     1381 gtcgctggcc gaaacagcgc cgcgcactgc gccgcgttcg cccaacatcc gcaaaccgag
     1441 acgatgcgtg ccgtcgcgcg cgacgtcgat gctcccttcg atgcgatcgg cgccgccgac
     1501 ggcagtcgtg acttgcacca cgccaccggc ggcattgccg tacaactgtg ccagcgggcc
     1561 gcgcaggact tcgatgcgtt ccgcactgcc cagatcgatg gcagacactt gtccctgacc
     1621 gtccggcatg gtggcgggaa tgccgtccac cagaatgcgc agaccgcgaa cgccgaaagc
     1681 ggagcgcgcg ccgaaaccgc gcaccgacaa ctgcagatcc tgcgcgtaat tctggcgctc
     1741 cagcgcgacg atgccgggca agcggcgcaa cggttcggaa atgttcaggc gcggaccggc
     1801 accgcggatt tcatccgctc cgacgctgtt gacggctgcg ggtgcgtcga aagcgcgctg
     1861 ttcatgacgg ctcgcggaaa cgacgatttc gtcgagcgca tcctgtgcct cgactgtcga
     1921 tgcggcgaaa acggcgagcg cgaagagtga aagcggggcg ggggatcgtc ctggcatgac
     1981 gagggcagca tagcaggcgg catgccgacg acttgccagg gcggcgttaa cgcagggtct
     2041 gcagcttctg ttcgacgaat tgcgccacgg cgggacgcag cgtgccggtg tcgcgcgcat
     2101 gcgcataagc atcggccgcc tcggcgggct gctgtgttgc ctcgtaagag cggccgaggc
     2161 cgacccacca gatgccggca tttggtgcaa acttcagcac gtcacgatac gcctcgatcg
     2221 cttcggcatg gcgatcgagc cgctgcagca gtgccgccgt gaaggcgcca tattcggcat
     2281 tcgcttgcgc ggcgtccgca tgcgcacgca gcacttgcag cgcgccgtga tcgtcgccgc
     2341 gatccagatg caggcgggcg agcacgatgg caaagttggt ctgtgccgga ttgatgcgca
     2401 aaccttcggc gagcaggcgc tcggcgtcgg cgttcgcacg cgcatcgatc agcagcacgg
     2461 cgagcgtctg tcgcgcggct tcgtgcgccg ggtcgagttc cagcgcctgc gtcagcgctg
     2521 tgcgtgcttc ctcgttgcgc ccccgattga tcagggctgc ggcgcggcga tattcgctgt
     2581 cggcggtgtc cgcgggtgtg tgcgggcgca cctcgatttt cgtcggaccg gcatcgcgtt
     2641 cggcaggcgc cgcctgtggc gtctgggcag ggcgttcgac gtttgcgacg acgtcggtcg
     2701 gcgcaggatc gacgggcacg gtcgatagga caggcgccgg ttcgacgacg ggccgcgagg
     2761 gctcgggttg cacttgcggc gctgcgggcg tttccgtcgc aggcgtttcc actgcggcga
     2821 tctgttgcga tacggccggc gcttcttccg gcgctgtggg tacgacaact gattcgacca
     2881 ccggggcgga cggcgaaggc gcttcggcct catcgttgcg cggcgtgtcg atggctgcga
     2941 cgggtgcctg gatatcgcga acgtgcgaat cgacgcttgc cggccggtcg aagtaccgta
     3001 gcgcgccata acttgccgcc agcacgagca tgaaggcgag tgcgcaggcg agccacaagc
     3061 cgcgacgtgt ggaggcgggc ggttgcacgt gctgggcgta gagcgcctgc tcgccggcct
     3121 ggcgccggtc gaggtcgcgc agcatctggt tcacgacgct catagtcttc cgatcatgac
     3181 aaagccggct gccgcaccca gcgtggcggc gcccaacagc cccagccacc acaacacgcg
     3241 ggtcggctgg cgcgcagcgg gcgtgtcgct cacggcgcgc agcgcatcgg cgcggcgaat
     3301 ctggtgcgcg ccggcgccgt aggccagcat caacgatttg tgggcgagga tgttggccag
     3361 acgcggaacg ccgcgactgg cgcgatgaat gacgcgcgtt gcgccgcgcg agaacaggag
     3421 gccgcccgca tagcccgcca ccaccaaccg gtgggcgagg taatacgcgg tttcatccag
     3481 actgagcggc gtcagacggt gctcgaaagt gatgcgctgg cgcagttggc gaatctcctg
     3541 acgcgccagc ttctcgtcga gttcgggctg gccgaacagc accacctgca gcagtttgcg
     3601 cttctcggtt tcgagattgg tcagcagcct caccatctcc agcgtttcga tcggcatcgc
     3661 ctgcgcttcg tccacgcaca acaccgggcg cttgcccgcc tgcgccagcg cgagcagatg
     3721 cgtatcgatg gcacgcatga ggctgtgctc gtcgtcgcct gccggcaatt ccgcgcccag
     3781 ttcgctcgcc acggcacgca gcagggtgtg cggtgagatg ttgggattgg gaatgtaggc
     3841 ggcgatccag tcctcgccca ggctgccgag gaacttgcgg cacaacagtg tcttgccggt
     3901 gccgacttcg ccggtgatct tgatgaagcc ctcgccgttg cgcacggcga acatgagcgt
     3961 gttgagcgcc ccctgcatgc cgcggcagga gcagaagaag ctcgtgtccg gcgtaatgct
     4021 gaacggcgcc tcgcgcagcc cgaagtgggc gaggtaaatc attagtcggc gacggccccg
     4081 ccgcggcgcg cgcccagact cagtagacgg tcacgcgccc cgccgatatc gtcgctgttg
     4141 gtctcgccgc cgatgatggt gggcttgatc aggatgacca gttccttctt caccgccacg
     4201 cggtcgcggc tgccgaacgc attgccgagc accggcacgt tctgcagacc gggcagaccg
     4261 gacttgctgt cgctgatatc gactttcatc agaccgccga tggcaacgat gttgccgtcg
     4321 gcgactttca ccacgctgtc ggtttcgctc accgtgctgc gggcgagcgg caggacgatg
     4381 ttctcgccga agagatcgcc cagattgatc tggcggttgt cctgcaccac attgctgacc
     4441 gacgggtgca cgtgaaggat tacgttcgat gcgtcgtcga tctgcggcgt gatgtcgagc
     4501 gacacgcccg agaagaaggg ctgcagcgtc agcgtcggga aggaggtcgt gttgccgccg
     4561 atgacgccac tggtggtgtt gccgctttgc acgttggtaa tgaagaattc gtcggtgccg
     4621 accttcaaca ccgctttctg gttgttgagc gcggcgatgc gcgggctgga cagcacctga
     4681 acgctgccct ggccctcaag gaattccagt acggcggcga aattgtgggt ctggaaggcc
     4741 aggccgaaca ggccgcccgg caagcctgca gcttcggtga cgctcgagac ggcgccggta
     4801 ccgatctcgc gcaccggatt ttccggagag gcgaaaatgc cgcggttgcg cgcagagatt
     4861 tcgccgacgt tcgacaattg cgcccccggc cccatcacgc cgccggcaaa cttgcccttg
     4921 ccgaacacgg cccagttgat gcctgcctga tacgcgtcgg acagcgtcac ttcgatgatc
     4981 ttcgcctcga tcatgacctg gcgttcgaca gacagtttga tggcgcgcag atagtcctgc
     5041 acgttgcgca tgtcggcggg catgccgcgc acgacgatga cgccggcttg cggattgacc
     5101 accacgttgc ggccgggctc ggtgccgacg atgaccttga gcgtttgttc gagatcgcgc
     5161 cagaagtccg tgctggtgga ggtcagcacg cgggcggagt cggtgacgcg gctggtgctg
     5221 ccctgtgtgc cgggctgacc gccgaccgcc gtcggaatgg ctgccgctgc ggccgccgcc
     5281 cccggcgatg cggtgcccgc gtcggcgacc gaaccggaaa tgacgcgcaa ctcgctccga
     5341 cccaagcgct ggccggccag atagttcacg cggaacacgc gcgtttgcag cgacgccgcc
     5401 tggacgatga tgcggttgcc gtcgatgcgg tattcgtagc cgtacagttc gcgcagcgct
     5461 tcgagcgcct cgggcacggt ggtgtccttg agatcgacgg tgattgtgcc ggcgacgtcc
     5521 ggatgcacca gcatgctgta gcgcgtgccc gagacgatgg cgagcagcac ctgttgcgcg
     5581 ggtgcgtcgt tgaccgacac atcgaagcgc ggctcgagtt cgcggtcatc gactttcggc
     5641 agttccagac gcagcggcgg aatgagtgcc tgatcgatcg ccgcgtcgcg cgccggctgc
     5701 ggcttggcgg ccgctgcggc gttgatctcg tcgacgatgc tgcggtcgag ggtctgcggg
     5761 tcgcggggga ccgcgcatgc ggtgagcgca caggcgagcg caaggatgag cgtcttcttc
     5821 attgcttcgt ctttctcccc gagagggcgt cgtggctgac ggttttcggc gccacgatct
     5881 gtttcttgat ttcaggcacg agcttgagca cggtttctcc ggcggcgtcg cgcagcgtca
     5941 cttcgttctc ggcgatgcgc acgagcttgg cctcgccgta gacgccgccg accgcaatgc
     6001 gctcgccgct gataatggcg tagcgccgtt ccttgctgat gattaccgac tgcaatacgg
     6061 ggccggcctc cggggcggat gccgtcacgc tgaattgcgg cgggcgcatg gggtcgttca
     6121 acgcctgcgc gtatgccgcc atcgtgccga gagccagcgc agcgaaggca taaagacgga
     6181 cgagcttcat acgaccaccc aggtgcgttc gaggctgagc gtatagacgc tgagcttcat
     6241 gctgatctgc ggatactcgt cgccggcgat ttccacttcg ccccagtaca ggcgcagcgg
     6301 caggcgttcg agttcgtcga ggtagcggag cagatcgaaa tagctgccgc gcacggtgag
     6361 ttcgacgccg tgccgataca cctgcagagc ggacacccgc gtgccgtcct gccggtcgcc
     6421 ggcgtgcgcc gaggcgtcga acagcggcgc gggcggcagc gactgcaggc cgaccagttc
     6481 cagcctgcgc tcgcgacgca gcatttcctc cagcagcgcc ggcacatgat cgggcgccac
     6541 cagttcgcgc tgtttttccg ccagacgagt atcgagctcg gcgatgcgct gcttgaggtc
     6601 atcgatctgc ttgcggcgct cgacgtccgg atcgcgtgtg ctttgcgcga cgaggatctg
     6661 gatctgatcc tgcagcttgc gcgtatcgag ttgctgctgg gcgagttgac gagccacttc
     6721 gcgttgacgc ttcgtgaccg gaccgctcaa cagagtgttg aataggaaga tcatcaccat
     6781 cgccgcggcg aaaaatacga ttgcccgttc gcgcagcacc atggcgtcga tccgttccga
     6841 ccacttgagc caccactgtt tcatcgggca cctcgtctgg ccggctgggt cgcgccgagc
     6901 gtgaactcga cgtagcggcc ttcggcggcg ttattgcgct tctcgacgtg cgaggtcagt
     6961 tgcagttcgg caaagcggtg g
//
LOCUS       DATGOU010000013         7172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_919955, whole genome shotgun sequence.
ACCESSION   DATGOU010000013 DATGOU010000000
VERSION     DATGOU010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919955"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VKZ48_00815"
     CDS             <1..217
                     /locus_tag="VKZ48_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HLU76424.1"
                     /translation="KSVGVMGDGRTYEYVVSLRAVQTTDFMTAHWAHLPYDLLGKVSN
                     RIINEVRGINRVVYDISGKPPATIEWE"
     gene            complement(222..800)
                     /locus_tag="VKZ48_00820"
     CDS             complement(222..800)
                     /locus_tag="VKZ48_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76425.1"
                     /translation="MNVLPSSPRAQSLAGLALLAILVSTPVRAALESSMSLHMLLQFP
                     GLMLGGALLSAAAPMRMRRLLAPWNELGISGLVAVVLSLTVLMIPRVLDLALVEPGVE
                     AAKIAALAACGALLRHSWHMGGLLIQAFFLGNLLPMMGVVGSLYDNAPIRLCNAYLLG
                     DQQRLGQGLVGLSIAIALIWLAYAARIVMRRP"
     gene            complement(797..1438)
                     /locus_tag="VKZ48_00825"
     CDS             complement(797..1438)
                     /locus_tag="VKZ48_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HLU76426.1"
                     /translation="MLRTALASACFAVLAYAAACASTYDFQVWTAEGARRLEVALHPV
                     GAPAVAMSTVAGTSEPLGRLLKDEHAATIVDFIYTRCATVCLSLGSTFQQLQAAILAA
                     YPDENSPPVRLLSISFDPAHDTPHALKQYAAGLGADPRVWRFAGVDDARHLPQLLQPY
                     QVVVVPDEFGGFEHNAALLVVDSAGRLVRIFDYAEADTALAYARSLARGTRAR"
     gene            complement(1485..2087)
                     /locus_tag="VKZ48_00830"
     CDS             complement(1485..2087)
                     /locus_tag="VKZ48_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLU76427.1"
                     /translation="MPNLLEPSHPAQAREHAEPSESLRPVPLVVVLVTVAMVIFGAGY
                     ILFSDPFGASALGDQRTLADLRPSAGAPTGQADGKQLFNAQCVACHQASGQGLPGVFP
                     PLDGSEWVSRDERILANILLYGINGELTVAGATYKGAMPAFGHLSDAELAALATYIRT
                     SWSNKAAPLTAALFTEERAAHPRNAPFTSAAELEALAVRP"
     gene            complement(2103..2693)
                     /locus_tag="VKZ48_00835"
     CDS             complement(2103..2693)
                     /locus_tag="VKZ48_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HLU76428.1"
                     /translation="MENELKLTAGAVTALAIAASALVVLPYIQVRDVEPAGDLKPYTE
                     TQLRGRHVYIANGCMYCHTQQPRDRKMGPDAERGWGRASVPGDYYYDKPHLLGSMRTG
                     PDLLNIGVRQPSKDWHLGHLYQPRAYVPDSIMPAYPFLFTIKSAAEEGDQVVNLPPGT
                     APDGKVVVANREALDLVAYLQGLDRTYPVLPPEPKP"
     gene            complement(2683..4332)
                     /locus_tag="VKZ48_00840"
     CDS             complement(2683..4332)
                     /locus_tag="VKZ48_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HLU76429.1"
                     /translation="MDSAVLSLLGAFLLSIIGLFVFIWSMRKGLLIENPRAASLIFAK
                     GEIGRVDDPALAAGEHERMQAAAVEAGDTGSQADPAEIEDRIASDRSSSFPVFMFIAF
                     ACFWLLIGSAAGLTSSIKLHQPDFLTEQAWLSFGRLRTIHLNAVMYGWVSNACLAVIV
                     WLLPRLLRTRLYGAMWTMLGGTFINAGIVAGIGAIAMGWTDGMEYLEIPWQIGIFIFA
                     GFALIILSALYTLANRRVEQLYVSVWYLSAALLWIALLFFVAKLPGVHTGVQQATTNW
                     WYGHTVLGLWLTPVSLGAIYYFLPKIIGRPIRSYNVSLLGFWTLAFFYGQVGGHHLIG
                     GPVPGWLVTLSIVQSVMMVIPVIAFTINQGGTLWGRMYLARYSPTLRFMMLGGLMYMI
                     SSLQGSLEALRSVNAVTHFTHFTVAHAHLGVYAFVSIVLFGSIYFMLPRVLSWEWPYP
                     KLITLHFWLVTIGIAVYVVSLTIGGWLQGTAMLDAARPFMDSVRLTLPYLEGRTVGGA
                     LMTLGHIVFVGHFFAMALRFGPHRTGAALFWQGKLEPALGK"
     gene            4827..5318
                     /gene="tadA"
                     /locus_tag="VKZ48_00845"
     CDS             4827..5318
                     /gene="tadA"
                     /locus_tag="VKZ48_00845"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HLU76430.1"
                     /translation="MALEDDAAHTAYMEEALRLAHQAWTLGEVPVGAVVVHAGQIVGR
                     GYNAPISRHDPTGHAEIMALRDAARNLQNYRLPGCELYVTLEPCAMCAGAIMHARIAR
                     VVYGARDPKTGVCGSAIDLFAEQRLNHHTEVVGGVMADQCGALLSGFFEQRRRMQKEA
                     DAH"
     gene            5308..5787
                     /locus_tag="VKZ48_00850"
     CDS             5308..5787
                     /locus_tag="VKZ48_00850"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HLU76431.1"
                     /translation="MRIEISIPEQRLTLYGDGGEAVKTYAVSTASKGAGEKQGSHCTP
                     RGRHIIRAKIGAGAPLDTVFVGRRPTGEIYTPELGARFPDRDWILTRILWLSGCEPGF
                     NRLGDVDTMRRYIYIHGAPDSAPMGVPGSIGCVRMHNRDLVELFDLVPAGTPVFIAG"
     gene            complement(5790..6800)
                     /gene="mtnA"
                     /locus_tag="VKZ48_00855"
     CDS             complement(5790..6800)
                     /gene="mtnA"
                     /locus_tag="VKZ48_00855"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HLU76432.1"
                     /translation="MANAVETLRWGGDHVEFIDQRKLPGRIEYVRCHDAQEVAAAIRD
                     MVVRGAPAIGCAAAYGVALEALKGTRDFTQAFAALRASRPTAVNLYWALDRMQALIDA
                     APPDLAATLVAEALAIHAEDLEINYAIGRNGLPYVPDGARVMTYCNTGALATAGHGTA
                     LGVIRSAHEAGKGISVLASETRPYLQGARLTAWELVQEHIPVTLITDNMAGHFMRTGQ
                     VDLVIVGADRIAANGDVANKIGTYGLAVLAARHNIPFYVAVPVPTFDPAIESGEAIPI
                     EERSAEEVVGYGNVRWAPEGVKVAHPAFDVTPAELVTALITDRGVIEKPDAAKVRQLL
                     GR"
     gene            complement(6803..>7172)
                     /locus_tag="VKZ48_00860"
     CDS             complement(6803..>7172)
                     /locus_tag="VKZ48_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLU76433.1"
                     /translation="VVAIGSKNVPAPGPGAAAYSASKAALTQLARVAALEWGADGIRI
                     NTLHPNAVFDTGIWTDDMLAERARRYGMSVEQYKTNNVLRTEVTSRDVAELAAELCGP
                     LFAKTTGAQIPVDGGNERVI"
BASE COUNT         1288 a         2244 c         2446 g         1194 t
ORIGIN      
        1 gaagtccgtc ggcgtgatgg gcgacggccg cacctacgaa tacgtggtga gtctgcgcgc
       61 cgtgcagacc accgacttca tgaccgcgca ctgggcgcac ctgccctacg acctgctggg
      121 caaggtttcc aaccgcatca tcaacgaagt gcgcggcatc aaccgcgtgg tgtacgacat
      181 ctccggcaag ccgcctgcga ccatcgagtg ggagtgagtt ctcagggccg ccgcatcacg
      241 atgcgcgcgg cgtacgccag ccagatcagc gcgatcgcga tactcagtcc gacgagcccc
      301 tggccgaggc gctgctggtc gccgagcaga taggcgttgc acaggcgtat cggcgcgttg
      361 tcgtacagcg agccgacgac gcccatcatg ggcagcaggt tgccgaggaa gaacgcctgg
      421 atcaacaatc ctcccatgtg ccaggagtgg cgcaaaaggg cgccgcatgc cgccagcgcc
      481 gcgatcttcg ccgcttcgac acccggctcg accagcgcca gatccaatac acgcggaatc
      541 atcaggaccg tcaggctcaa gacaaccgcg acaaggccgc tgatgcccaa ctcgttccat
      601 ggtgcaagca gacggcgcat acgcatcggc gctgcggcgg acagcagcgc gccgccgagc
      661 atcagccccg gaaactgcag cagcatgtgc aggctcatgg acgattccag ggcagcgcgc
      721 accggcgtcg acaccaggat cgccagcagt gcaagacccg ccagactctg cgcgcggggg
      781 cttgaaggga gtacgttcat cgcgcgcgag ttccccgtgc gagcgagcgt gcgtaggcca
      841 gcgccgtatc ggcttcggcg taatcgaata tgcgcaccaa gcgacccgcg gaatccacca
      901 ccagcagcgc cgcattgtgt tcgaaaccgc cgaattcgtc gggtacgacc acaacctgat
      961 aaggctgaag cagttgcggc agatgccgcg catcgtccac gccggcgaag cgccacacgc
     1021 gcgggtcggc acccaggccg gccgcgtatt gcttcaaggc atgcggggtg tcgtgcgccg
     1081 gatcgaagct gatcgaaagc agccgcaccg gcggcgaatt ctcgtccggg tatgcggcga
     1141 ggatggccgc ctgcagttgt tggaaggtgc tgcccagcga aaggcagacc gtcgcgcaac
     1201 gcgtatagat gaagtcgaca atggtcgccg cgtgctcatc cttcagcagg cggccgagtg
     1261 gctccgaggt gccggcaacg gtggacatgg cgacggctgg cgcgccgacc gggtgcagcg
     1321 ccacttcgag ccgtcgagcg ccttccgctg tccagacctg gaagtcgtag gtcgatgcgc
     1381 aggccgccgc gtaggcgagg acggcaaagc aggcgctggc cagtgcggtg cgcagcataa
     1441 ggctcaaccc gtgccggggc tgtaccgcgc cggtgcgcgc gcgctcaagg ccggacggcg
     1501 agcgcctcga gctccgctgc gctcgtgaac ggcgcgttgc gcggatgcgc ggcgcgctcc
     1561 tcggtgaaca gcgcagccgt caacggtgcc gccttgttcg accagctcgt gcgtatgtag
     1621 gtggcgagcg cggcaagttc ggcatcgctg agatggccga aggcgggcat ggcgcccttg
     1681 taggtggcgc cggcgacagt gagctcgccg ttgatgccgt acagcaggat gttcgccagg
     1741 atgcgctcgt cgcggctcac ccattccgag ccgtcgagcg gcgggaacac gcccggcagt
     1801 ccttgtccgc tggcctgatg gcaggctacg cattgcgcat tgaacaactg cttgccgtcg
     1861 gcctgcccgg tgggcgcgcc ggccgaggga cgcaggtcgg ccagcgtacg ctggtcgccg
     1921 agtgcggagg cgccgaaggg atcggaaaat agaatgtagc ccgcgccgaa aatcaccatc
     1981 gcgacggtga cgagcacgac gacgagcggc accgggcgga ggctttccga gggttcggca
     2041 tgttcgcgcg cctgggccgg atgagaaggt tcgaggaggt tgggcataag ctcgatgcgc
     2101 gactagggtt tgggctcggg gggcagcacg gggtaggtgc ggtcgagtcc ctgcagatag
     2161 gccaccagat ccagtgcttc ccggttggcg acgacgacct tgccgtccgg tgcggtgccg
     2221 ggcggcagat tcaccacctg atcgccttcc tcggcagcgc tcttgatcgt gaacaggaac
     2281 gggtaggccg gcatgatcga atcgggcaca taggcgcgcg gctggtacag atggccgagg
     2341 tgccaatcct tgctcggttg gcgcacaccg atattgagca gatccggacc ggtgcgcatg
     2401 ctgccgagca gatgcggctt gtcgtagtag tagtcgcccg ggacggaggc gcggccccag
     2461 ccgcgttcgg catccggtcc cattttgcgg tcgcgcggct gctgggtatg gcagtacatg
     2521 caaccgttgg caatgtagac gtgacggccg cgcaactgcg tttcggtata gggcttcagg
     2581 tctcctgccg gctccacgtc gcggacctgg atatacggca gtacgacgag cgccgaggcg
     2641 gcgatggcca gcgcggtcac cgcgccggca gtgagcttga gttcattttc caagcgcggg
     2701 ctccagtttt ccttgccaga acagcgccgc gccggtgcgg tgcggaccga agcgcagcgc
     2761 catagcgaaa aagtggccga caaacacgat gtggccgagc gtcatcagcg cgccgccgac
     2821 ggtgcggcct tcgagatagg gcagggtcag gcgtaccgag tccatgaacg gccgcgcggc
     2881 atcgagcatt gccgtgcctt gcagccagcc gccgatggtc agggacacga cgtagaccgc
     2941 gatgccgatg gtgaccagcc agaagtggag cgtgatcagt ttcggatatg gccactccca
     3001 gctcagtacg cgcggcagca tgaagtagat cgagccgaac agcacgatgc tgacgaaggc
     3061 gtacacgccc agatgggcat gggccacggt gaagtgggtg aaatgggtga cggcgttgac
     3121 gctgcgcagc gcttcgagcg aaccctgcag cgacgagatc atgtacatca ggccgccgag
     3181 catcatgaag cgcagcgtgg gggaatagcg cgccagatac atgcgtcccc acagcgtgcc
     3241 gccctggttg atggtgaagg cgatgaccgg gatcaccatc atgacgcttt gcacgatcga
     3301 aagcgtgacc agccagcccg ggacggggcc gccgatgaga tggtgcccgc cgacctgccc
     3361 gtaaaagaac gccagggtcc agaagccgag cagcgagacg ttgtacgagc ggatcggccg
     3421 gccgatgatc ttgggcagga aatagtagat cgcgcccagg ctgaccggcg tgagccagag
     3481 accgagcacg gtgtggccgt accaccagtt ggtggtggcc tgctgaacgc cggtgtgaac
     3541 gccgggcagc ttcgccacga aaaagagcag ggcgatccac agcagcgcgg cgctcagata
     3601 ccacacgctc acgtagagct gttcgacccg acggttggcg agcgtgtaca gggccgagag
     3661 gatgatcagc gcaaagcccg cgaaaatgaa gatgccgatc tgccagggaa tttccagata
     3721 ttccatgccg tcggtccagc ccatcgcgat ggcaccgatg ccggccacga tgcccgcatt
     3781 gatgaacgtg ccgcccagca tggtccacat ggcgccgtac agccgggtgc gcagcaggcg
     3841 cggcaggagc cacacaatga ccgccaggca ggcgttcgac acccagccgt acatcaccgc
     3901 gttgagatga atggtgcgca ggcggccgaa gctgagccat gcctgttcgg tcagaaagtc
     3961 cggctgatgc agcttgatcg atgaggtgag gccggcggcg gaaccgatga gcagccagaa
     4021 gcaggcgaag gcgatgaaca tgaaaaccgg aaaggaactc gagcggtccg aagcgatgcg
     4081 gtcctcgatt tccgccggat cggcttgcga tccggtatcg cccgcttcga cggcggcggc
     4141 ctgcatccgc tcgtgctcgc cggcggccag cgccggatcg tcgacccggc cgatttcgcc
     4201 tttggcgaaa atcagcgatg cggcgcgcgg attctcgatc agcaacccct tgcgcatcga
     4261 ccagatgaaa acgaagagcc cgatgatgga cagcaggaat gcccccaaca ggctgagtac
     4321 ggcactatcc atgaagcgct tcctccgtgt gccggtcgga gtgctgcgcg catgggattc
     4381 gttcagtgaa catcgaccac cttttgcacg agatgcacga tggcgaacat gtcgccgcgc
     4441 tcgccgtcat tcgcctgtgt cggcgctttg cgcgatgaag gttcatcgcg cgacggatcg
     4501 gcattgactt gaaaggcagt gcgggtcagc cggcgcggtt gccggacgtg gccacatcgt
     4561 catgcgtatg acgattcggc attgaccttg gaaggaatgt cggtcggccg ccgggtgcgt
     4621 accgggtctg gcgagcgcat ttgaccagga cgatgcacct tgcggttgct ttttaggcta
     4681 ggcacgcgtt tgcagtgtgg atttgatcta gctcaaatca gatcgttttt ccttgctcga
     4741 tacgggctgc gatagttgtc gtctatacaa cgttcgcact ggatgtaccg ggctgccgcg
     4801 tcgaatgatc ttcggtatgc ttcggtatgg ctttggaaga cgacgctgct cacaccgcat
     4861 acatggaaga ggcgctgcgc ctcgcgcacc aggcgtggac gctcggtgaa gtgccggtag
     4921 gcgcggtggt agtgcatgcg gggcagatcg tcgggcgcgg ctacaacgcg cccatttcgc
     4981 gccacgatcc cacgggccat gcggagatca tggcgttgcg cgatgcggcg cgcaatctgc
     5041 agaactaccg gctcccgggg tgcgaactct acgtcacgct cgaaccctgt gccatgtgtg
     5101 cgggcgccat catgcatgcg cgcatcgccc gcgtcgtgta cggcgcgcgc gatcccaaga
     5161 ccggcgtctg cggcagcgcg atcgatctgt tcgccgagca gagactcaac caccatacgg
     5221 aagtcgtggg cggcgtgatg gccgaccagt gcggcgcgct gctgagcggt tttttcgagc
     5281 agcgccggcg catgcagaag gaagccgatg cgcattgaaa tctccatccc cgagcaacgg
     5341 ctgacgctct acggcgacgg cggcgaggcg gtgaagacct atgccgtctc gacggcaagc
     5401 aagggcgcag gcgagaagca gggcagtcat tgcacgccgc gcggccgtca catcatccgc
     5461 gcgaaaatcg gcgccggcgc gccgctcgat acggtgttcg tggggcgccg cccgaccggc
     5521 gaaatctata cgcccgaact gggcgcgcgt tttcccgacc gcgactggat cctgacgcgc
     5581 atcctgtggc tttcggggtg cgagccgggc ttcaaccggc tgggcgacgt ggacaccatg
     5641 cgccggtaca tctatatcca cggtgcgccg gacagcgcac cgatgggcgt tccgggctcc
     5701 atcggctgcg tgcgcatgca caaccgcgat ctggtggaat tgttcgatct ggtgccggca
     5761 ggcacgccgg tgtttatcgc cggctgagtt cagcggccga gcagttgccg tactttggcc
     5821 gcatccggtt tttcgatgac gccccggtcg gtgatcaacg ccgtcaccag ttccgccggc
     5881 gtgacgtcga acgccggatg cgcgaccttc accccctccg gcgcccagcg tacgttgccg
     5941 tagccgacga cttcctcggc gctgcgttcc tcgatgggaa tggcttcgcc gctttcgatg
     6001 gcggggtcga aggtcggtac cggcacggcg acatagaacg gaatgttgtg ccgcgcggcc
     6061 agtaccgcca ggccgtaggt gccgatcttg ttcgccacgt cgccgttcgc cgcaatccgg
     6121 tcggcgccga cgatcaccag gtccacctgg ccggtgcgca tgaagtggcc ggccatgttg
     6181 tcggtgatga gtgtgacggg aatatgctcc tgcaccagtt cccacgcggt cagtcgcgcg
     6241 ccctgcagat aggggcgcgt ctcgctggcg agcacgctga tgcctttgcc tgcctcgtgc
     6301 gcggagcgga tgacgcccag cgcggtgccg tgccccgccg tcgccagggc gccggtgttg
     6361 cagtaggtca tgacgcgcgc gccgtcgggc acatagggca ggccgttgcg gccgatggcg
     6421 taattgatct ccagatcttc ggcatgaatt gccagcgctt ccgcgacgag cgtcgcggcg
     6481 agatccggcg gcgcggcgtc gatcagcgcc tgcatgcgat caagcgccca atagagattg
     6541 accgcggttg ggcggctggc gcgcaacgcg gcgaaggctt gcgtgaagtc gcgcgtgccc
     6601 ttcaacgctt ccagcgcgac gccgtatgcc gccgcgcagc cgatggccgg cgcgccgcgc
     6661 accaccatgt cgcggatcgc ggccgccact tcctgtgcgt catgacagcg cacgtactcg
     6721 atgcgtcccg gcagcttgcg ctggtcgatg aattccacgt gatcgccgcc ccagcgcagc
     6781 gtctcgactg cattcgccat ctttaaatca ctcgttcgtt gccgccgtcc acgggaattt
     6841 gcgcacccgt ggtcttggcg aatagcgggc cgcacagctc ggcggcgagt tccgcgacat
     6901 cgcggctcgt gacctcggtg cgcagcacgt tgttggtctt gtactgctcg accgacatgc
     6961 cgtaacgccg tgcgcgttcg gccagcatgt catcggtcca gatgccggtg tcgaatacgg
     7021 cattgggatg cagcgtgttg atgcggatgc cgtccgcacc ccattcgagt gcggccacgc
     7081 gcgcaagttg cgtgagcgcc gccttcgagg cggaataggc cgccgcgccc gggccgggcg
     7141 cggggacgtt cttcgagccg atcgccacga cg
//
LOCUS       DATGOU010000014         6554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_483448, whole genome shotgun sequence.
ACCESSION   DATGOU010000014 DATGOU010000000
VERSION     DATGOU010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483448"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1047
                     /gene="murA"
                     /locus_tag="VKZ48_00865"
     CDS             <1..1047
                     /gene="murA"
                     /locus_tag="VKZ48_00865"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HLU76434.1"
                     /translation="QGVILNAASLREPVASYELVKTMRDSILVLGPLLARYGRARVSL
                     PGGCAIGQRPVDQHVRGLQAMGASIQVERGYINATAPRLKGAHLILDMVTVTGTENLM
                     MAAALAEGRTVLENAAREPEVVDLANCLNAMGAKISGAGTDVITIEGVESLHGAQHRI
                     MPDRIETGTFLVATAATGGAVTLRDADASLLEAVIDKLREAGARVQTSGDSIRVEMSG
                     RPQSVNVRTAPYPAFPTDMQAQFMALDAIAEGAGVITETIFENRFMHAQELVRLGADI
                     ETSGNTAIVRGVPKLEGAKVMATDLRASASLVIAGLVANGETVIDRIYHLDRGYEAIE
                     EKLSRLGARIRRVR"
     gene            1171..1821
                     /gene="hisG"
                     /locus_tag="VKZ48_00870"
     CDS             1171..1821
                     /gene="hisG"
                     /locus_tag="VKZ48_00870"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009932070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HLU76435.1"
                     /translation="MSGITIALSKGRIFDETAPLLAAAGIAPLENPETSRKLIIGTNR
                     PDVRLVIVRASDTPTYVQYGAADLGIAGKDVLYEHSGEGLYQPIDLEIAKCRMMVAVP
                     DGFDYESAVRKGARLRVATKYLRIAREHFAAKGVHVDLIKLYGSMELAPLVGLADVIV
                     DLVSTGGTLKANNLRAVEEIMPISSRLIVNQAALKLKRPLLQPVIDAFESAVNAHA"
     gene            1814..3118
                     /gene="hisD"
                     /locus_tag="VKZ48_00875"
     CDS             1814..3118
                     /gene="hisD"
                     /locus_tag="VKZ48_00875"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HLU76436.1"
                     /translation="MPEIKRLSTAQSDFDAQLAALTALEAAQDENVDRAVADILADVR
                     KRGDAAVLEYTARFDRVQAKSMAELEIPRAALHAALDKLPAERRVALEQAAERVRSYH
                     ERQRMESWSYVEADGTLLGQQVTPLDRAGLYVPGGKAAYPSSVLMNALPAKVAGVGEL
                     IMVVPTPDGEKNELVLAAAAICGVDRVYAIGGAQAVGALAYGTDTVPQVDKIVGPGNA
                     YVAAAKRRVFGVVGIDMIAGPSEILVVCDGQTDPDWIAMDLFSQAEHDEVAQSILISP
                     DAAFLDRVAASIDRLITEMPRRTVIETSLANRGALILVRDLDEACEIANRIAPEHLEL
                     SVADPDRWVGKIRHAGAIFMGPYTSESIGDYCAGPNHVLPTSRTARFSSPLGVYDFQK
                     RSSLIKVSREGADTLGRIAATLAYGEGLPAHARSAEYRLRDK"
     gene            3118..4194
                     /gene="hisC"
                     /locus_tag="VKZ48_00880"
     CDS             3118..4194
                     /gene="hisC"
                     /locus_tag="VKZ48_00880"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HLU76437.1"
                     /translation="MGAAPRNPQALVREDILALKAYHVAPATGMVKLDAMENPYGLPD
                     DLRAELGELAAATALNRYPDPTAPELRARLRKAFDIPADAGLLIGNGSDELITLVTQT
                     LARPDAVMLAPEPSFAMYRMNAIYSRMRYVGVPLAEDFSLDLGRFLAAVEEHRPALIF
                     IAYPNNPTGNLYPERDIEAIIEASSGYVVVDEAYHAFAGKSFMDRLSRYPNLMVMRTV
                     SKLGLAGIRLGYAAARPEWIEQFDKVRPPYNVNVLTQAVAARMLDRIDVLNAQAARIR
                     TDRATLDAGLRALPGVRVFPSEANFILARVPDADELYRRLEARKVLVRNLHGSHPLLA
                     QCLRFTVGTPEENEQLLTALRASL"
     gene            4211..4804
                     /gene="hisB"
                     /locus_tag="VKZ48_00885"
     CDS             4211..4804
                     /gene="hisB"
                     /locus_tag="VKZ48_00885"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003815795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HLU76438.1"
                     /translation="MRTAQITRNTNETQISASINLDGTGKGNLDTGVPFLDHMLDQIV
                     RHGLIDIDIKAKGDLHIDAHHTVEDVGITLGQAFANAVGDKKGLRRYGHAYVPLDEAL
                     SRVVIDFSGRPGLEFHVDFTRPLIGDFDVDLVREFFQGFVNHALVTLHVDNLRGVNSH
                     HQCETVFKAFARALRAALEVDPRASGTVPSTKGTLSA"
     gene            4881..5522
                     /gene="hisH"
                     /locus_tag="VKZ48_00890"
     CDS             4881..5522
                     /gene="hisH"
                     /locus_tag="VKZ48_00890"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HLU76439.1"
                     /translation="MSAIAVVDYGMGNLRSVAKAIEHVAPHAQVRVTSDPADVLDAER
                     VVFPGQGAMRDCMREMDARGLRPSLLEAARTKPFLGICIGLQMLFDHSEEGGGVPGLA
                     ILPGQVRRFPADRMQAADGSRLKVPHMGWNNVHQVREHALWRGIADRSRFYFVHSYYV
                     VPDVTELVTGSTEYAFPFTCAVARDNIFAVQFHPEKSHAAGLQLLSNFVSWKP"
     gene            5588..6349
                     /gene="hisA"
                     /locus_tag="VKZ48_00895"
     CDS             5588..6349
                     /gene="hisA"
                     /locus_tag="VKZ48_00895"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HLU76440.1"
                     /translation="MVIIPAIDLKDGRCVRLKQGDMDDATIFSEDPVGMARHWLELGA
                     RRLHVVDLNGAVAGKPKNEAIIRQIVDEVGEDLPIQLGGGIRDLDTIERYIDCGVSYV
                     VIGTAAVKNPGFLQDACAAFHGHIIVALDAKDGKVAVEGWSKMTRHAVVDLAQKFQDY
                     GVEAILYTDIGRDGMLTGVNIESTVSLAQQLRIPVIASGGLNDLDDVRALCAVEQEGV
                     MGVITGRAVYEGTLDFKAAQELADQLTAEAEDEEA"
     gene            6381..>6554
                     /locus_tag="VKZ48_00900"
     CDS             6381..>6554
                     /locus_tag="VKZ48_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012362933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HLU76441.1"
                     /translation="MSLAKRIIPCLDVTSGRVVKGVNFVELRDAGDPVEIARRYDEQG
                     ADELCFLDITASSD"
BASE COUNT         1157 a         2174 c         2115 g         1108 t
ORIGIN      
        1 cagggcgtga ttctcaacgc cgcatccctg cgcgaacccg tcgcctccta cgagctggtc
       61 aaaaccatgc gggactccat tctggtgctg gggccgctgc ttgcgcgcta cggtcgcgcg
      121 cgggtgtcgc tgccgggagg atgtgccatc ggtcagcggc cggtcgacca gcacgtccgg
      181 ggtctgcagg ccatgggtgc aagcatccag gtcgaacgcg gctacatcaa cgcgaccgcg
      241 ccgcgcctga aaggcgcgca tctgatcctg gacatggtca ccgtcacggg caccgagaat
      301 ctgatgatgg ccgcagcgct ggccgagggt cgcacggtgc tggaaaacgc tgcgcgcgaa
      361 cccgaagtcg tcgatctggc gaactgcctg aacgcgatgg gcgcgaaaat cagcggcgcg
      421 ggtaccgacg tgatcaccat cgaaggggtc gagtccctgc acggtgcgca gcatcgcatc
      481 atgcccgacc ggatcgagac cggtacgttt ctggtcgcca ccgcggcgac gggcggcgca
      541 gtcacgctgc gcgacgccga cgcctcgctg ctggaagcgg tgatcgacaa gttgcgcgag
      601 gcgggcgccc gggtgcagac cagtggcgac agcattcgcg tcgaaatgag cggccggccg
      661 caaagcgtca atgtccgcac cgcgccctac ccggcctttc ccaccgacat gcaggcgcaa
      721 ttcatggcgc tggacgccat cgccgaaggc gcgggcgtta tcaccgagac gattttcgag
      781 aaccgcttca tgcatgcgca ggaactggtg cggctgggcg cggatatcga gacctccggc
      841 aacaccgcca tcgtccgcgg ggtgcccaaa ctggaaggcg cgaaggtcat ggctaccgat
      901 ctgcgcgcct cggccagcct ggtcatcgcc ggcctggtcg ccaacggcga gacggtgatc
      961 gaccgcatct accatctgga ccgcggctac gaggcgatcg aggagaaact ctcgcggctc
     1021 ggcgcccgca tccgccgggt gcgctgaagg cccgatcccg tcgagcgcgt tacccggccg
     1081 ggctttcgag tagaatccga gctttagcgc tggcagtgcg cggggctgcg cgccctcgcg
     1141 agccttcttc ggcctctgac catttacccc gtgtccggca tcaccatcgc cctgtccaag
     1201 ggccgcattt ttgacgagac ggcgcctttg ctcgccgccg ccgggattgc tccgctggag
     1261 aatcccgaaa cttcgcgcaa gctcatcatc ggcaccaacc gccccgacgt gcggctggtc
     1321 attgtgcgcg cctcggacac gccgacctac gtgcagtacg gtgctgccga tctggggatc
     1381 gccggcaagg acgtgctcta cgaacacagc ggcgaggggc tttaccagcc catcgacctg
     1441 gaaatcgcca agtgccgcat gatggtcgcg gtgccggacg gcttcgatta cgaatccgcc
     1501 gtgcgcaaag gcgcgcgcct ccgcgtcgcc accaagtatc tgcgcatcgc ccgcgagcac
     1561 ttcgccgcga agggcgtgca cgtcgatctc atcaaactct acggctcgat ggaactggcg
     1621 ccgctggtcg gtctggccga tgtcatcgtc gatctggtca gcaccggcgg cacactgaag
     1681 gcgaacaacc tgcgcgccgt cgaagagatc atgcccatca gctcgcggct gatcgtcaat
     1741 caggcggcgc tcaagctcaa gcgtccgctg ctgcagccgg taatcgacgc cttcgaatcc
     1801 gcagtgaacg cccatgcctg agatcaaacg cctctccacc gcccagtccg atttcgacgc
     1861 gcaacttgcc gcgctcactg cgctggaagc cgcgcaggac gaaaacgtcg atcgggcggt
     1921 tgccgacatc ctggccgacg tgcgcaagcg cggcgacgcg gcggtgctcg aatacaccgc
     1981 gcgcttcgac cgcgtacagg ccaagtcgat ggcggaattg gaaatcccgc gcgccgcgtt
     2041 gcacgcggcg ctggacaaac tgccggccga gcgtcgcgtc gcgctggagc aggcggccga
     2101 acgcgtgcgc agctatcacg agcgccagcg catggaatcg tggagctacg tcgaggccga
     2161 cggcacgttg ctgggccaac aggttacgcc gctcgaccgc gcggggcttt atgtgccggg
     2221 cggcaaggct gcgtatccgt cttccgtact gatgaatgcg ctgcccgcca aggtggccgg
     2281 cgtcggcgaa ctcatcatgg tggtgcccac gccggatggc gagaagaacg aactggtgct
     2341 cgccgccgcg gcgatctgcg gcgtggaccg cgtgtacgcc atcggcggcg cgcaggcagt
     2401 cggcgcgctc gcatacggca ccgacaccgt gccgcaggtg gacaagatcg tcggcccggg
     2461 caatgcctac gtcgccgccg ccaagcgacg tgtattcggc gtggtcggca tcgacatgat
     2521 tgccggcccc tcggagattc tcgtcgtgtg cgacgggcag accgatccgg actggatcgc
     2581 catggacctg ttctcccagg cagagcacga cgaggtggca cagtcgatcc tcatttcgcc
     2641 cgacgccgcc ttcctcgatc gcgtcgcggc cagcatcgac aggctgatta cggagatgcc
     2701 gcgccgtacg gtcatcgaga cctcgctcgc caatcgcggt gcgctcattc tggtgcgcga
     2761 tctggacgaa gcctgcgaaa tcgccaaccg catcgcgccc gagcacctgg agttgtcggt
     2821 cgccgatccc gatcgctggg tgggcaagat ccgccacgcg ggcgcgatct tcatggggcc
     2881 gtacacctcc gagtcgatcg gcgactactg cgccgggccc aatcacgtgc tgcccacctc
     2941 acgcaccgca cgcttctcgt ccccgttggg cgtgtacgac ttccagaagc gttcgagtct
     3001 catcaaggtg tcgcgcgagg gcgccgatac gctgggtcgc atcgccgcca ccctcgccta
     3061 cggcgaggga ctgcccgcgc acgcccgctc ggccgagtac cggctgcggg acaagtaatg
     3121 ggcgccgcgc cccgaaatcc gcaggcgctg gtgcgcgagg acatcctcgc gctgaaggcc
     3181 tatcacgtcg cgcccgccac cggcatggtc aagctggacg cgatggagaa cccctacggc
     3241 ctgccggacg acctgcgcgc cgagttgggg gaactcgcgg cagcgacggc cttgaaccgc
     3301 taccccgacc ccaccgcgcc ggaattgcgc gcgcgcctgc gcaaggcatt tgatattccg
     3361 gctgacgccg gactgctgat cggcaacggc tcggacgaac tcatcaccct ggtgacgcag
     3421 acgcttgcgc gcccggatgc cgtgatgctc gcgcccgaac cgtccttcgc gatgtaccgc
     3481 atgaacgcga tctactcgcg catgcgctac gtcggcgtgc cgctggcgga ggatttcagt
     3541 ctcgatctcg ggcgcttcct cgccgccgtc gaagaacacc ggccggcgct gatcttcatc
     3601 gcctatccga acaaccccac gggcaacctt tatcccgagc gcgatatcga ggcgatcatc
     3661 gaagcctcgt cgggatatgt cgtcgtggac gaggcctatc acgccttcgc cggcaagagc
     3721 ttcatggacc gcctgtcgcg ctaccccaat ctgatggtga tgcgcacggt gtcgaagctg
     3781 gggctggccg gcatccggct cggttatgcg gccgcgcgcc ccgagtggat cgagcagttc
     3841 gacaaggtgc ggccgccgta caacgtcaac gtgctcacgc aggcggtggc ggcccggatg
     3901 ctggaccgga tcgacgtgct gaacgcccag gcggcgcgga ttcgcaccga ccgcgcaacg
     3961 ctcgacgcgg gcttgcgcgc actgccgggc gtccgggtgt ttcccagcga agcgaacttt
     4021 atactggcgc gcgtgcccga tgcggatgaa ctgtatcgcc gcctcgaagc gcgcaaggta
     4081 ctggtgcgca acctgcacgg cagccatccg ctgctcgcgc agtgtctgcg cttcaccgtc
     4141 gggacgcccg aggagaacga acaactgctg accgccttgc gcgccagcct gtaggaccgg
     4201 ggagcatgga atgagaaccg cacagatcac acgcaacacc aacgagacgc aaatcagcgc
     4261 ctccatcaat ctcgacggga ccggcaaggg caatctcgat accggcgtgc ccttcctcga
     4321 tcacatgctc gaccagatcg tgcgccacgg cctgatcgac atcgacatca aggcgaaggg
     4381 cgatctgcac atcgacgcgc accacacggt ggaagacgtc ggcatcacgc tcggccaggc
     4441 ctttgccaac gcggtgggcg acaagaaggg cctgcgccgc tacggccatg cctatgtgcc
     4501 gctcgacgaa gcgctgtcgc gcgtggtgat cgacttttcg ggccgcccgg gcctggagtt
     4561 ccatgtcgat ttcacgcgtc cgctgatcgg ggacttcgat gtggacctcg tgcgcgaatt
     4621 cttccagggc ttcgtcaacc acgccctggt gacgctgcac gtggacaacc tgcgcggcgt
     4681 gaattcgcac catcagtgcg aaaccgtgtt caaggccttt gcgcgcgcat tgcgcgcggc
     4741 gctcgaggtc gatccgcgcg catccggcac ggtgccgtcc acgaaaggga cgctgtccgc
     4801 atgagttcga tcgccgtagt cgactacggt gtagcacgct tcgaggcgaa ggcttacgtt
     4861 cgcacagagg gctgcgcggc atgagcgcga tcgccgtcgt ggactatgga atggggaatt
     4921 tgcgctcggt cgccaaggcc atcgagcatg tggcgccgca tgcgcaagtt cgcgtcacga
     4981 gcgatccggc cgatgtgtta gatgcagagc gggtggtgtt cccgggccag ggcgcaatgc
     5041 gcgactgcat gcgcgagatg gatgcgcgcg ggttgcgtcc gtcgctgctg gaagcggcgc
     5101 gaaccaagcc gttcctcggt atctgcatcg gtttgcaaat gctgttcgat cacagcgagg
     5161 agggcggcgg tgtgccgggg ctcgccatat tgcccgggca ggtacgccgt tttccggccg
     5221 atcgcatgca ggcggcggac ggcagtcgtc tcaaggtgcc gcacatgggg tggaacaatg
     5281 tgcatcaggt gcgcgaacat gcgctctggc ggggcattgc ggaccgcagc cgcttttatt
     5341 tcgtccacag ctattacgtg gtcccggatg tgacagagct tgtcaccggc agcaccgagt
     5401 acgcgtttcc ctttacctgt gcggtggctc gggataacat ttttgccgtc cagttccatc
     5461 ccgagaagag ccacgcggcc gggctgcagt tgctatcgaa tttcgtgtcc tggaagccct
     5521 gactccgaac acgtctttta cgcgaattcc aagactcctt tccacccacg cgaaccgctg
     5581 acgcgcgatg gttattattc ctgcaatcga tctcaaagac ggccgctgtg tccgcctcaa
     5641 gcagggcgac atggatgacg ccaccatttt ttccgaagac ccggttggca tggcccgtca
     5701 ctggctcgaa ctgggcgcgc gacggctgca tgtcgtcgac ctcaacggcg cggttgccgg
     5761 caagccgaag aacgaagcca tcatccggca gatcgtcgat gaagtcggcg aagacctgcc
     5821 gatccagttg ggcggcggca ttcgcgatct cgacacgatc gagcgctaca tcgactgcgg
     5881 tgtgagctac gtcgtgatcg gcaccgccgc agtgaagaat cccggcttcc tgcaggacgc
     5941 ctgtgccgcc ttccacggac acatcatcgt cgccctcgat gccaaggacg gcaaggtggc
     6001 ggtcgaaggc tggtcgaaaa tgacccggca cgccgtggtc gacctggcgc agaaattcca
     6061 ggactacggc gtcgaggcca ttctctatac cgatatcggc cgcgacggca tgctcaccgg
     6121 cgtcaatatc gagtcgaccg tttcgctggc acagcagttg cgcattcccg tcatcgccag
     6181 cggtggactc aacgacctgg acgacgtgcg cgcgttgtgc gccgtggagc aggaaggcgt
     6241 gatgggcgtc atcaccggcc gcgcggtgta cgaaggtaca ctcgacttca aggcggcaca
     6301 ggaactggcg gaccagctca ccgccgaagc agaggacgag gaagcctgat cccgccccgc
     6361 gggccgctgc ttccgtcacc atgagtcttg ccaaacgcat cattccctgc ctggacgtca
     6421 ccagcggccg ggtggtcaag ggcgtcaatt tcgtcgaatt gcgcgacgcg ggcgatcccg
     6481 tcgaaattgc gcgccgctac gacgagcagg gtgccgatga actgtgcttc ctcgacatca
     6541 ccgcgagctc cgac
//
LOCUS       DATGOU010000015         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_5815018, whole genome shotgun sequence.
ACCESSION   DATGOU010000015 DATGOU010000000
VERSION     DATGOU010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5815018"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VKZ48_00905"
     CDS             complement(<1..213)
                     /locus_tag="VKZ48_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76442.1"
                     /translation="MMQQYLRLKAQYPDLMLFYRMGDFYELFFDDAEKAARLLDITLT
                     TRGQSSGAPIKMAGVPFHAVEQYLARL"
     gene            complement(287..1075)
                     /locus_tag="VKZ48_00910"
     CDS             complement(287..1075)
                     /locus_tag="VKZ48_00910"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HLU76443.1"
                     /translation="MHPTLNIAVKAARRAASVINRASTQLDLLTVQSKSPNDFVTEVD
                     RAAEAAIIEVLREAYPEHAILAEESGRSGDSEYCWIIDPLDGTTNFIHGFPQYAISIA
                     LARNDVLEQAVIFDPVRNELFAASKGGGAFLNDRRIRVSRRIKLMDSLVGTGFPFRQN
                     DNIEAYIGAFRELSQKTAGIRRPGAAALDLAYVACGRLDGFWEAGLSSWDMAAGALLI
                     MEAGGLVSDFGGDSAYLDTGRLVAGTPKVFAQLLPIVQAHMGSK"
     gene            1212..1940
                     /locus_tag="VKZ48_00915"
     CDS             1212..1940
                     /locus_tag="VKZ48_00915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HLU76444.1"
                     /translation="MQDTSLLDRIRVVLTRTSHPGNIGAAARAMKTMGLSRLVLVAPD
                     SFPDPVADARASGALDVLNAAVVVPTLADALQGTVFSAALTARRRELSLPLQMPRQAA
                     PELVRYAQGGDVALVFGTESSGLTNDEVALCGMPVTIPANPAYSSLNLAAAVQLLCYE
                     LRLAAADETAPQTPPEDAARFEDVEGFYAHLEDAMRDSGFYDPATSRRLLPRLRRLFG
                     RIRLERDEINILRGIVAALQRKPD"
BASE COUNT          358 a          662 c          684 g          371 t
ORIGIN      
        1 cagtcgcgcc agatactgtt cgacggcatg gaagggcaca cccgccatct tgatgggcgc
       61 gccactggac tggccgcgcg tggtcagcgt gatgtcgagc aggcgcgcgg ccttctcggc
      121 gtcgtcgaag aacagctcgt agaagtcccc catgcgatag aacagcatca ggtccgggta
      181 ctgcgccttg aggcgcaggt attgctgcat catcggcgtg tggccggcga tggaggcttc
      241 gtcgaaggct ggctttgtgg cggtctgggt caaggcgggc aggggtttat ttgctgccca
      301 tgtgcgcctg cacgatcggc aacagttgtg cgaagacctt cggtgtgccg gctaccagac
      361 gcccggtgtc gagataggca ctgtcgccgc cgaagtcgga caccagaccg ccggcttcca
      421 tgatcagcag cgctccggct gccatgtccc agctgctcag gccggcttcc cagaagccgt
      481 cgaggcggcc gcaggcaaca taggccagat ccagcgcggc cgcgcccgga cgacggatgc
      541 ccgcagtttt ctgcgacagt tcgcggaagg caccgatata ggcttcgatg ttgtcgttct
      601 ggcggaacgg aaagcccgta ccgaccagcg aatccatcag cttgatgcga cgcgagaccc
      661 ggatgcggcg atcgttcagg aaggcgccgc cacctttcga tgcggcgaac agctcgttgc
      721 gcactggatc gaagatcacg gcctgctcga gcacgtcgtt gcgcgccagc gcgatggaga
      781 tggcgtactg cggaaagccg tgaatgaagt tggtcgttcc gtcgagcgga tcgatgatcc
      841 agcagtattc gctgtcgccg gagcggcccg actcctctgc tagaatcgcg tgctccggat
      901 aggcctctcg cagcacttcg atgatcgcgg cctcggcggc gcgatcgacc tcggtgacga
      961 aatcattggg cgatttcgat tgcacggtga gcaggtcgag ctgtgtcgag gcgcggttga
     1021 tcacactcgc ggcgcgacgg gcggccttga cggcgatgtt cagggtggga tgcatggaac
     1081 ggttctcggt tgctgcggat tcaccatcga agcccgcaga aatgatgatc ttgaatagcc
     1141 gcgcattcta accgcaaacc ccctccttac ggtcccgaat gcggggccgt aacacccgaa
     1201 tcaggcatcc gatgcaagac acttcgcttc tcgaccgcat ccgagtggtg ctcacgcgca
     1261 ccagtcatcc cggcaacatc ggggctgccg cgcgcgccat gaagaccatg ggactgtcgc
     1321 gcctggtgct ggtggcgccg gactcgtttc cggatccggt cgccgatgca cgcgcgtccg
     1381 gcgcgctgga cgtactgaat gcggccgtgg tggtgccgac gctggccgat gcgctgcaag
     1441 gcacggtgtt ctcggcagcg ctcaccgcgc gccggcgtga actgtcattg ccgctgcaga
     1501 tgccccgcca ggccgcgccg gaactggtgc gctacgcgca gggcggcgac gttgcgctgg
     1561 tattcggcac cgaatcgagc ggactgacca acgacgaggt cgcgctgtgc ggcatgccgg
     1621 tgacgattcc ggccaacccc gcgtactcct cgctgaacct cgccgcggcc gtgcagctgc
     1681 tgtgttatga gctgcgtctg gcagcggcgg acgaaacggc gccgcagacg ccgccggaag
     1741 acgctgcgcg gtttgaagac gtggaaggct tctacgccca cctcgaggac gccatgcgtg
     1801 actcgggctt ctacgacccg gccacctcac gccgcctgct gccgcgtctg cgtcggctgt
     1861 tcgggcgcat tcgcctcgaa cgcgacgaga tcaacatcct gcgcggcatc gtggctgcat
     1921 tgcagcgcaa acctgattaa aatactcgac tattgaagct gtctgcgcca gcaccggcgc
     1981 gccaataccc gggagacacc gtccatgttc agccgcctgc gtgaagacct tgccagcgtt
     2041 cgcgagcgcg accccgccgc gcgttcgacc tggga
//
LOCUS       DATGOU010000016         6563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_1818441, whole genome shotgun sequence.
ACCESSION   DATGOU010000016 DATGOU010000000
VERSION     DATGOU010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818441"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VKZ48_00920"
     CDS             <1..703
                     /locus_tag="VKZ48_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HLU76445.1"
                     /translation="DWKYAYLRDVVSMSVLPETVSAFRVQRERWGRGLIHSGFKHVRK
                     MLRQSMPFMKRLHAIATMFSSVLLASIYVLVLLSLPLNYLVDFTGTNIHWVALGFYAL
                     VAVWALDNAFGAYKGARFEDRPGLFRLCIDAYLYVALFLPMAWYYFAGGIRALFGVYG
                     DFHRTPKGREERRAPMPPINGVLLAGEIFTFAYSLIAVLVAWQQGNYFLLPLNLTVCL
                     GFGMVLRWSWLERRG"
     gene            774..1574
                     /locus_tag="VKZ48_00925"
     CDS             774..1574
                     /locus_tag="VKZ48_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLU76446.1"
                     /translation="MSNHERRRARRTSLDAGNRRILISGATGAIGAALADAYARPGVT
                     LYLHGRNETRLAEVAERCRTKGAEVMTQRFDLREFATLQTWLQGLEPLDLVIVNAGVN
                     THVGSSGEPEPWDAAEALLDVNLKAAMLMTQAVLPGMRTRGRGQIALISSLAAYFGLP
                     VTPAYCASKAGLKAYGEALRGWLAPEGIRVNVVMPGYVQSPMCDDMPGPKPFLRSPER
                     AARAIVRGLERDRARISFPFPLNLGTWLLAVMPAGLSTRILRWMGYGA"
     gene            1564..3168
                     /locus_tag="VKZ48_00930"
     CDS             1564..3168
                     /locus_tag="VKZ48_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HLU76447.1"
                     /translation="MALEAGFAMVPVAALTALLLSVGIERLMTPRPSLARPPSAWLLH
                     AGLLLCAHALLTLLLGRPWFAAAALLALLLTVVLVNNAKVRTLDEPFVFQDYEYFTDA
                     VRHPRLYIPFLGWWKFLGAAAGVVLAVAVGLWWEAAPVSRFAWSGQLGGVALVFVVGA
                     LSLVIGARKPLAVTFDAARDVREFGLIASMWRYAMEERAPAVESHSPFVVSREPVERS
                     NHERETPDLPHLVAVQSESFFDARALYAGIRADVLAEFDRLCADALLSGKLSVPAWGA
                     NTVRSEFAFLSGIGEAQLGVHRFNPYRAVAAGWEAPSLAASLRRLGYRTVCIHPYPAS
                     FYRRDRVYPKLGFDEFVDIRAFDTAARFGPYIGDAAVADKVASLLENATQPVFVFVIT
                     MENHGPLHLEHVAPTDIDALYREAPPAGCEDLTIYLRHLRNADRMIGTLRSALEQSAR
                     PASLCWFGDHVPIMPAVYDNFGKPKGEVHYVCWSNRHSAAGEHSDLAAHDLAIAWLRV
                     LGLVVNPEDSDQSLDFETTSRRAQAG"
     gene            3285..3917
                     /gene="queE"
                     /locus_tag="VKZ48_00935"
     CDS             3285..3917
                     /gene="queE"
                     /locus_tag="VKZ48_00935"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007733358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HLU76448.1"
                     /translation="MTYAVKEAFLTLQGEGANAGLAAVFCRFAGCNLWTGREEDRRNA
                     VCRFCDTDFVGTDGVNGGKFRTADELADHVAQLWQGADEERFVVCTGGEPLLQLDAAL
                     IDALHARGFRIAVESNGTIEAPDGIDWLCISPKAGSEVVQRRGNELKLVYPQTGMDPA
                     HFADWDFEHFYLQPLDDARQAQNTAAAVEYCLRHPRWRLSLQTHKLIGIP"
     gene            4025..5275
                     /gene="hemA"
                     /locus_tag="VKZ48_00940"
     CDS             4025..5275
                     /gene="hemA"
                     /locus_tag="VKZ48_00940"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HLU76449.1"
                     /translation="MQPLFTLGLNYQTAPLAIRERVAFGAEQLRHALAEIFRAKPARE
                     AAIISTCNRTELYCAAGDPLEALDWLAAYHGVDPQDLKPHLYTLPREDAVRHAFRVAS
                     GLDSMVLGEAQILGQIKDAVRTAEEVGTLGVTLSRLFQRSFAVAKEVRTQTRIGANVV
                     SMAAAAVKLAGRIFPSLRDQRVLFIGAGEMIELAATHFAAQLPREMAIANRTLERGQA
                     LANRLGATPLMLSELPDRLAQYDIVVSCTASTLPILGKGTMERVIKQRRHAPVFMVDL
                     AVPRDIEPEVGALDDVYLYTLDDLAEIVRAGIDERQSHVAQAEAIIDAQVGSFLHWME
                     SRALVPLIRGLRAQGEAARKQEIERAMRLLARGESPEAVLEALSHGLTNKLLHGPTQA
                     LHDAGPAEREELQRMLARIYRLGE"
     gene            5416..6498
                     /gene="prfA"
                     /locus_tag="VKZ48_00945"
     CDS             5416..6498
                     /gene="prfA"
                     /locus_tag="VKZ48_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011979439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HLU76450.1"
                     /translation="MKASLTLKLDQLVRRLEELNALLSSENATSDMDEYRRLSREHAE
                     ITPVVERYREYKAAKDDAQAAKDMMADPEMRAFAESELEAAEERMEALELDLQKLLLP
                     RDPNDERNIFLEVRAGTGGEESALFAADLLRMYTRYAERQRWQVEVISESPSDLGGYR
                     EVIVRLAGHDVYSRLKFESGGHRVQRVPETEAQGRIHTSACTVAVLAEADEVEDVVLN
                     PADLRIDTFRASGAGGQHINKTDSAVRVTHLPTGIVVECQDDRSQHKNKARALAVLAA
                     RIKDVQMREQQSKEAAARKSLIGSGDRSERIRTYNFPQGRMTDHRINLTLYKLDFIMD
                     GDLNELTTALTTEHQAELLASLADAS"
BASE COUNT         1099 a         2099 c         2184 g         1181 t
ORIGIN      
        1 cgactggaag tacgcctatc tgcgcgatgt ggtgtcgatg tcggtgctgc ccgagacggt
       61 cagcgccttc cgcgtccagc gcgagcgctg gggacgcggg ttgatccaca gcggcttcaa
      121 acacgtgcgg aagatgctgc gccaaagcat gccgttcatg aaaaggctgc acgcgatcgc
      181 cacgatgttt tcgtccgtgc tgctcgcctc gatctacgtg ctcgtgctgc tcagtctgcc
      241 gctcaactat ctggtcgatt tcaccggcac gaacatccac tgggttgcgt tgggtttcta
      301 cgcgctggtc gcggtatggg cgctggataa tgccttcggc gcctacaagg gcgcgcggtt
      361 cgaggaccgg cccggtctgt tccgcctgtg catcgacgcc tatctgtatg tcgcgctgtt
      421 cctgcccatg gcctggtact acttcgcggg cggcatccgc gcgctgttcg gcgtgtacgg
      481 cgacttccat cgcacgccca agggcaggga ggaacgtcgc gcgcccatgc cgccgatcaa
      541 cggcgtgctg ctggcgggcg aaatcttcac cttcgcctat tcgctgatcg ccgtgctcgt
      601 cgcgtggcag cagggcaact actttctgct gccgctgaac cttacggtct gcctgggttt
      661 cggcatggtg ctgcgctggt cctggctgga gaggcgcgga tgaacaaggc gaataaaact
      721 cggattgagc ccttcgacag cgcgaacggt aaaggaggcc gctcgtggtg agcttgtcga
      781 accatgagcg gcgtcgcgcc cgtcgcacat ctcttgacgc gggcaatcgc cggattctca
      841 tcagcggggc aaccggcgcc atcggcgcgg cgttggcaga cgcctatgcg cgaccgggcg
      901 tgacacttta tctgcacggc cgcaacgaaa cccgcttggc cgaggtggcg gaacgctgca
      961 ggacgaaggg cgccgaggtg atgacgcagc gattcgacct gcgggagttc gcgaccctgc
     1021 agacatggct gcaaggtctg gagccgctcg atctggtcat cgtcaacgcc ggcgtgaaca
     1081 cgcatgtcgg atcgtcgggc gagcctgaac cctgggatgc ggcggaggcg ttgctggacg
     1141 tgaacctgaa agcggcgatg ttgatgacgc aggcggtgtt gccgggcatg cgcacccgcg
     1201 gtcgcggaca gattgcgctg atcagttctc tggcggcgta tttcggcctg cctgtgacgc
     1261 cggcctattg cgcgagcaag gccgggctca aggcctatgg cgaagctctg cgcggctggc
     1321 ttgcgcccga aggcatacgc gtcaatgtcg tcatgccggg ttacgtgcaa tcgccgatgt
     1381 gcgacgacat gccgggaccc aaaccgttcc tccggtcgcc cgaacgggcg gcgcgcgcca
     1441 tcgtgcgcgg actggagcgc gaccgggcgc gcatcagctt cccctttccg ctgaacctcg
     1501 gcacctggct gctggccgtc atgccggcgg gtctttcgac gcgcatcctg cgctggatgg
     1561 gttatggcgc ttgaggccgg cttcgcgatg gtgccggtcg cggcgctgac cgcattgctg
     1621 ttgtcggtcg gcatcgagcg tctgatgacg ccgcggccga gcctcgcccg accgccgagc
     1681 gcctggctgc tgcacgcggg actgttgctc tgcgcgcatg cgctgctcac gctgctgctc
     1741 gggcggccct ggttcgccgc cgccgcgctc ctggctttgc tgctcacagt ggtattggtg
     1801 aacaacgcga aggtccggac gctggacgag cctttcgtgt tccaggacta cgaatacttc
     1861 accgacgccg tccgccatcc gcgtttgtac atcccgtttc tgggctggtg gaaatttctc
     1921 ggcgcggccg caggcgtggt gctcgccgtt gcggtgggct tgtggtggga ggctgcgccg
     1981 gtttcgcgct tcgcctggtc cggtcaactg ggcggcgtgg ctctggtctt cgtcgtcggc
     2041 gcgctgtcgc tggtaattgg cgcacgcaaa ccgcttgcgg tgacgttcga tgcggcacgc
     2101 gatgtgcgcg agttcggatt gatcgcgtcg atgtggcgct atgcgatgga agagcgcgca
     2161 ccggcggtgg agtctcattc tccgttcgtg gtgagccgtg agcctgtcga acggtcgaac
     2221 catgaacggg aaacaccaga cctcccgcac ctcgtcgccg tccagagcga atccttcttc
     2281 gacgcccgcg cgctttatgc gggtatccgt gccgatgtgc tcgccgagtt cgatcgtctg
     2341 tgcgccgacg cgctgctcag cggcaagctt tcggtgcccg cctggggtgc gaacacggtg
     2401 cgcagcgaat tcgcctttct gtcgggtatc ggtgaagcgc aactcggcgt acatcgcttc
     2461 aacccttatc gcgccgtcgc cgccgggtgg gaagcgccgt cgctggccgc gtcgctcagg
     2521 cggctcggct atcgcaccgt gtgcatccat ccctatccgg cgagtttcta ccggcgggac
     2581 cgcgtctatc cgaagctggg cttcgacgag ttcgtggaca tccgcgcttt cgacacggcg
     2641 gcgcgattcg gcccgtatat cggcgacgcc gccgtggccg acaaggttgc ctcgctcctg
     2701 gaaaatgcga cgcagccggt cttcgttttc gtcatcacca tggaaaacca tggccccctg
     2761 catctggagc acgtcgcgcc gacggacatc gacgcgctgt accgcgaggc gccgccggcc
     2821 ggctgcgagg atctgaccat ctacctgcgc catctgcgca atgccgaccg aatgatcggc
     2881 accctgcgca gcgcgctgga acaatccgcc cgtccggcaa gtctgtgctg gttcggcgac
     2941 cacgtgccca tcatgcccgc cgtttacgac aacttcggca aaccgaaggg cgaagtccac
     3001 tacgtctgct ggagcaaccg ccatagcgcg gcgggtgagc acagcgactt ggctgcccat
     3061 gacttggcga tcgcttggct tagggtgctc ggcttggttg tgaaccccga ggattcggac
     3121 cagagcttgg atttcgaaac gacgtcgagg cgcgcacaag cgggatgagc gagtgcgcac
     3181 ggcatcggtc gctcctcgcg gaacgctata ctgcggccga cactcatccg gtttttccct
     3241 aacttcccat agcgtcgtgc cgttcgaaaa cggccaccgc cccaatgact tacgccgtca
     3301 aagaagcttt tctgaccctc cagggcgagg gggccaacgc cggtcttgct gcggtgttct
     3361 gccgttttgc gggatgcaac ctgtggacgg gccgcgagga agaccggcgc aacgcggtgt
     3421 gccggttctg cgatacggac ttcgtcggca ccgacggcgt caacggcggc aagttcagga
     3481 cggccgacga gctggcggat cacgtcgcac aactgtggca aggcgcggac gaggagcgct
     3541 tcgtggtgtg tacgggcggg gagccgctac tgcagctcga tgcggcgctg atcgacgcgc
     3601 tgcatgcgcg cggcttccgc attgccgtcg aatccaacgg cacgatcgag gcgccggacg
     3661 gcatcgactg gctgtgcatc agcccgaagg ccggcagcga ggtggtgcag cggcgcggca
     3721 acgaactgaa gctggtctat ccgcaaacgg gcatggaccc ggcgcatttc gcggactggg
     3781 acttcgagca cttctatctg cagccgctcg atgacgcacg acaggcgcag aacacggccg
     3841 cggcggtcga gtactgtcta cggcaccccc ggtggcgact gagcctgcag acgcacaagc
     3901 tgatcgggat tccctgagag ccgcccggtc gggccataag tccttgtctc aaacgtagaa
     3961 acgcgcgttt tgcggtcggt gcgcgacagc cttccggtat aatcgggtcg cccaatcgac
     4021 cgccatgcaa ccgctcttca cattaggtct caattaccag accgcgcccc tcgccattcg
     4081 cgagcgcgtg gcgttcggtg ctgagcaact gcggcacgcc ctggcggaga tcttccgcgc
     4141 caagccggcg cgcgaggcgg ccattatttc cacctgtaac cgcacggaac tctactgtgc
     4201 ggccggcgat ccgctggagg cgctcgactg gctggcggcc tatcacggcg tcgacccgca
     4261 ggacctgaaa ccgcatctct acacgctgcc gcgcgaggat gcggtgcggc atgccttccg
     4321 cgtcgcttcc gggttggact cgatggtgct cggggaggcg cagatcctcg gtcagatcaa
     4381 ggacgccgtg cgcacggccg aagaagtggg cacgctgggc gtcacgctgt cgcgcctgtt
     4441 ccagcgttcc ttcgcggtgg ccaaggaagt gcgcacgcaa acgcgcatcg gtgcgaacgt
     4501 cgtgtccatg gccgctgcag cggtgaagct tgccggccgc atcttcccgt cgttgcgcga
     4561 ccagcgcgtc ctgttcatcg gcgcgggcga gatgatcgaa ctggccgcca cgcatttcgc
     4621 cgcgcaactg ccgcgcgaga tggcgatcgc caaccgcacg ctggaacgcg gtcaggcgct
     4681 ggccaaccgt ctgggcgcga cgccgctcat gttgtcggag ttgccggacc ggctggctca
     4741 atacgacatc gtcgtgtcct gtacggcgag cacgctgccc atcctcggca aggggacgat
     4801 ggaacgcgtg atcaagcagc gccgtcatgc gccggtgttc atggtcgatc tggcggtgcc
     4861 gcgcgatatc gaacccgaag tcggcgcgct ggacgacgtc tatctctaca ccctggacga
     4921 tctcgccgag atcgtgcgcg ccggaatcga cgagcggcag tcgcacgtgg cgcaggccga
     4981 ggcgatcatc gacgcgcagg tcggctcctt cctgcactgg atggaatcgc gtgcgctggt
     5041 gccgctcatc cggggcctgc gtgcgcaggg cgaagcggcg cgcaaacagg aaatcgaacg
     5101 cgcgatgcgg ctgctcgcgc gcggcgaatc gcccgaagcg gtgctggagg cgctgtcgca
     5161 cggcctcacc aacaaactgc tgcacggtcc gacgcaggcg ctgcacgatg ccggcccggc
     5221 cgagcgcgaa gaactgcagc gcatgctggc ccgcatctat cgcctgggcg agtagggccg
     5281 cggaatactg gatcagtgtg atgccgggcg agcccggata ggttttcgtc attccggacg
     5341 cctccgccca gcggaggccg atccggaatc cattgaaggc ggcggaatca gcgccttccg
     5401 agcagtacgg aatttatgaa agcaagtctc accctcaagc tcgaccagtt ggttcgacgg
     5461 ctggaagagc tcaacgcgct gctgtcctcc gaaaacgcaa ccagcgatat ggacgagtat
     5521 cgccgcctga gccgcgagca cgcggaaatc acgccggtgg tggaacgcta tcgcgagtac
     5581 aaggcggcga aggacgatgc gcaggccgcg aaagacatga tggccgaccc ggagatgcgc
     5641 gcctttgccg aaagcgaact ggaagcggcg gaggagcgga tggaggcgct ggagctggat
     5701 ttgcagaagc tgctgctgcc gcgcgatccc aacgacgaac gcaatatctt tctggaagtg
     5761 cgcgccggta cgggcggcga ggaatccgcc ctgttcgctg ccgatctgct gcgcatgtat
     5821 acccgctacg ccgagcggca gcgctggcag gtcgaggtca tttccgagag tccttccgat
     5881 ctgggcggct atcgggaggt gatcgtgcgg ctggccgggc acgacgtgta ttcgcgcctc
     5941 aaattcgagt ccggcgggca tcgcgtgcag cgcgtgccgg aaaccgaggc gcaggggcgc
     6001 atccacacct ccgcgtgcac ggtggcggtc ctggccgaag cggacgaggt cgaggacgtg
     6061 gtgctcaatc ccgccgatct gcgcatcgat accttccgtg cctcgggtgc gggcggacag
     6121 cacatcaaca agaccgattc ggccgtgcgc gtgacgcact tgcccaccgg catcgtggtc
     6181 gagtgccagg acgaccgctc gcagcacaag aacaaggcgc gtgcgctcgc cgtgctggcc
     6241 gcgcgcatca aggacgtgca gatgcgcgag cagcagtcga aggaagccgc cgcgcgcaag
     6301 tcgctgatcg gctcgggcga ccgcagcgag cgcatccgca cctacaattt cccgcagggc
     6361 cgcatgaccg accaccgcat caatctgacg ctgtacaagc tcgacttcat catggacggc
     6421 gacctgaatg aactgaccac tgcactgacg accgaacatc aggcagaatt gctggcatcg
     6481 ctggccgacg ccagctgagc actgaaacca aatgcgcacc gacccactct acttcggcat
     6541 tcccttacgg accgtccaat gaa
//
LOCUS       DATGOU010000017         5695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_5820851, whole genome shotgun sequence.
ACCESSION   DATGOU010000017 DATGOU010000000
VERSION     DATGOU010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5695
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5820851"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..229)
                     /locus_tag="VKZ48_00950"
     tRNA            complement(143..229)
                     /locus_tag="VKZ48_00950"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(193..195),aa:Leu,seq:caa)
     gene            327..1382
                     /gene="queA"
                     /locus_tag="VKZ48_00955"
     CDS             327..1382
                     /gene="queA"
                     /locus_tag="VKZ48_00955"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HLU76451.1"
                     /translation="MSAPQQQNELTVEDFDYHLPPELIAQHPPAERTASRLLRLAPEG
                     PRDLMFGDLPDLLSAGDVLVFNDTRVIKARLRGTKSTGGQVEVLVERVLNAHEALAHV
                     RASKSPKPGTVLRLADAFDAQTLGRVDDLFHLRFDPAHTVFEWLEQYGHVPLPPYIDH
                     PDSAEDAQRYQTVYAATPGAVAAPTAGLHFDEAMLDRLRAKGVELAFVTLHVGAGTFQ
                     PVRVQRIADHKMHREWYEIPQTTVDAIARTREAGHKVIAVGTTSLRALESAAAMEGGL
                     RAGTGETELFITPGYRFRVIDRLLTNFHLPRSTLLMLVSAFAGMEPIRRAYRHAVAEH
                     YRFFSYGDAMLLDRAAG"
     gene            1397..2518
                     /gene="tgt"
                     /locus_tag="VKZ48_00960"
     CDS             1397..2518
                     /gene="tgt"
                     /locus_tag="VKZ48_00960"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HLU76452.1"
                     /translation="MAPMKFTLLHTDGAARRGTLELAHGTVQTPVFMPVGTYGSVKAM
                     SPADLTGLAAQIVLGNTFHLWLRPGLEVIEAHGGLHRFMGWTGPILTDSGGFQVFSLG
                     ALRKISEEGVRFQSPVNGDRLLLTPEESMRIQRVLNSDVVMIFDECTPYPASTGQAAE
                     SMRLSLRWAERSKRAHEGNANALFGIVQGGMYEELRDESLRGLMDIGFDGYAIGGLSV
                     GEPKEDMLRILAHTTPQLPAGRPRYLMGVGTPEDIVAAVAHGVDMFDCVMPTRNARNG
                     WLFTRWGDIKIRNARHRTDTRPLDETCACYTCRHFSRAYLHHLQRVNEILGARLATLH
                     NLHYYHCLMAELRAAIEAGRLDDYIARFRGERATLAADG"
     gene            2645..2911
                     /gene="yajC"
                     /locus_tag="VKZ48_00965"
     CDS             2645..2911
                     /gene="yajC"
                     /locus_tag="VKZ48_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HLU76453.1"
                     /translation="MQFVPIILMFVLLYFLMIRPQMKRAKEHKAMTEALTKGDEVVAA
                     GGVLGRITKVSDNYVTLEIAANTEVVVQRAAVQTLLPKGTIKGL"
     gene            2968..4827
                     /gene="secD"
                     /locus_tag="VKZ48_00970"
     CDS             2968..4827
                     /gene="secD"
                     /locus_tag="VKZ48_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HLU76454.1"
                     /translation="MNRYPLWVYITVAVALVVGLIYTLPNFYGESPAVQISSARATVK
                     IDESTLERAEKALADAGIAYDGARMDPTGVKVRFADTDTQLRARDLLDQAFNPDPSAP
                     DHTVALNLLPASPNWLSAIGAQPMYLGLDLRGGVHFLLQVDMQGAIIKRLDSYTGDIR
                     SMLRDARVRHSGVSREGSTVVIRFRDSQTRAEASKVLTRDLPDLMLNEVGTESQPRIV
                     ATIRPEAQKQIQEYALRQNIQTLHNRINELGVAEPVIQQQGADRIVVQLPGVQDTARA
                     KEILGRTATLEIRMVDEENMTPDRLAAAARGEVPFGSEYYVTREGQPLLIKRQVVLTG
                     DRLTNAQAGFDGMTNEPAVHLTLDATGARIFRDVTRENVGRRMAILLFEKGRGEVVTA
                     PVIRDEIGGGRVQISGQMNTSEANDVALLLRAGSLAAPMDIVEERTIGPSLGKDNIER
                     GFQSTMWGFIAVVVFMCMYYALFGVFSSIALAFNLLLLIALLSMLQATLTLPGIAAIA
                     LTLGMAIDANVLINERIREELRAGATPQAAIHAGYERAFGTILDSNLTTMIAALALLI
                     FGSGPVRGFAVVHALGILTSIFSSVVGSRSLVNLWYGSRRKVERLAIGNTSWK"
     gene            4858..>5695
                     /gene="secF"
                     /locus_tag="VKZ48_00975"
     CDS             4858..>5695
                     /gene="secF"
                     /locus_tag="VKZ48_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011852175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HLU76455.1"
                     /translation="MEFFKIRRDIPFMRHALAFNIISLVTFLLAVFFIWHKGLHFSIE
                     FTGGTVMEVSYSEAADVQRIRETLENAGYNDPQVQNFGSSRDVLIRLPLREDQSSAQV
                     SQAVMQKLQADNAGAQLQRVEFVGPQVGRELAENGALALLITCLGIMIYLAFRFEWKF
                     GLATVIANLHDVVIILGFFAFFQWEFSLPVLAAVLAVLGYSVNESVVIADRIRENFRK
                     MRKTPVTDVINNAITRTISRTIITHGSTQMVVLAMLFFGGPALHYFAMALTIGICFGI
                     YSS"
BASE COUNT         1013 a         1895 c         1816 g          971 t
ORIGIN      
        1 ggagtggtgc cgggagcgag aatcgaactc gcacggtgtc accaccgacg gattttggct
       61 ccgacataac gcggcttgca gccgcattag tctccgccga aatcccgcgg tagcttctaa
      121 ggtatgcaga agctgtaaga agtggtgccg ggagcgagaa tcgaactcgc acggtattgc
      181 taccgcggga ttttgagtcc cgtgcgtcta ccagttccgc catcccggct tgggaattcg
      241 tcgcggaggg cgtagcatcg cgcagtccgt gatccggacg cacccaccgc acaccgccgc
      301 ttcggaagga agcgcattat ccaagaatgt ccgcccccca gcaacaaaac gaactgaccg
      361 tcgaggattt cgactatcac cttccgcctg aattgattgc gcagcatccg ccggcggagc
      421 gtacggccag tcgtctgttg cgccttgccc cggaagggcc gcgcgatttg atgttcggcg
      481 acctgcccga tctgctttcg gcgggcgatg tactggtgtt caacgacacg cgcgtgatca
      541 aggcgcgact gcgcggtacg aagagcaccg gcggacaggt cgaggtgctg gtcgagcgcg
      601 tgctgaacgc gcatgaggcg cttgcccacg tgcgtgcgag caaatcgccc aagccgggca
      661 cagtgctgcg actggccgat gccttcgatg cgcagacact cggccgcgtg gacgatctgt
      721 tccacctgcg cttcgatccg gcgcacacgg tattcgagtg gctggagcag tacggtcacg
      781 tgccgctgcc gccgtacatc gatcacccgg acagcgcaga ggacgcccag cgctatcaga
      841 ccgtctatgc cgcaacaccc ggcgcggtgg cggcgccgac cgccggcctg catttcgacg
      901 aggcgatgct cgaccggttg cgggccaagg gcgtcgagtt ggcgttcgtg accctgcacg
      961 tgggcgcggg cactttccag ccggtgcgcg tgcagcgcat cgccgatcac aaaatgcatc
     1021 gcgagtggta cgagatccct cagactacgg tcgatgcgat cgcccggaca cgcgaagcgg
     1081 gccacaaggt catcgcagtg ggcaccacga gcctgcgcgc gctggagtcc gccgcggcca
     1141 tggaaggagg cctgcgcgcc ggcacgggcg aaacggaact tttcatcacg cccggctatc
     1201 gcttccgcgt gatcgaccgg ctgctgacca acttccatct gccgcgctcg acgctgctga
     1261 tgctggtctc ggccttcgcc ggcatggaac cgatccgccg cgcctaccgg catgcggtgg
     1321 cggagcacta tcgcttcttc agctacggcg atgcgatgct gctcgaccgc gccgcgggct
     1381 gacgccgccc gctacaatcg ccccgatgaa attcacgctc ctccacaccg acggcgcggc
     1441 gcggcgcggc acgctcgaac tcgcgcacgg cacggtccag acgccggtgt tcatgccggt
     1501 cggcacctac ggcagcgtca aggcgatgag cccggccgat ctgaccggac tcgccgcgca
     1561 gatcgtgctc ggcaacactt tccatctgtg gctgcgcccg ggactggaag tgatcgaagc
     1621 gcacggcggg ctgcatcgct tcatgggctg gacggggccg atcctcaccg attcgggcgg
     1681 cttccaggtg ttcagtctcg gcgcactgcg caagatcagc gaggaaggcg tgcgcttcca
     1741 gtcgccggtg aacggcgacc ggctgctgct cacacccgag gaatcgatgc gcatccagcg
     1801 ggtgctgaat tccgacgtgg tgatgatttt cgacgaatgc acgccctacc ctgcgagcac
     1861 cgggcaggcg gccgagtcga tgcgcctgtc cctgcgctgg gccgagcgca gcaaacgcgc
     1921 gcatgaaggc aacgccaacg cgctgttcgg catcgtgcag ggcggaatgt acgaggaact
     1981 gcgcgacgaa tcgctgcgcg ggctgatgga catcggcttc gacggctacg ccatcggcgg
     2041 cctgtcggtc ggcgaaccga aggaagacat gctgcgcatc ctcgcccaca ccacgccgca
     2101 actgccggcc ggtcggccgc gctatctgat gggtgtgggc acgccggagg acatcgtcgc
     2161 agcggtcgcg cacggtgtgg acatgttcga ctgcgtcatg ccgacccgca atgcccgcaa
     2221 cggctggctg ttcacccgct ggggcgacat caagatccgc aacgcccgcc accgcaccga
     2281 tacgcgcccg ctggacgaga cctgcgcgtg ctacacctgc cgccatttca gccgcgccta
     2341 tttgcatcat ctgcaacggg tcaacgaaat cctcggcgcg cgcctggcga cgctgcacaa
     2401 tctgcactat taccactgtc tgatggccga actgcgtgcg gccatcgagg ccggccggct
     2461 ggacgactac atcgcccgtt tccgcggtga acgggccacc ctggcggccg acggctaaaa
     2521 cgggggcccg tcagggggcc tcggttatac ttgcgcgctt tcccgcatcc tttttggaga
     2581 ctgaaccgtg ctgattagcg acgcattcgc acaagccggc ggcgcgccgg ccggcggcgg
     2641 cctcatgcag ttcgtgccga tcatcctgat gttcgtgctg ctgtacttcc tgatgatccg
     2701 gccgcagatg aaacgcgcca aggaacacaa ggcgatgacc gaggcgctga ccaagggcga
     2761 cgaagtcgtg gccgcgggcg gcgtactggg ccgtatcacc aaagtcagcg acaactacgt
     2821 aacgctggaa attgccgcaa acaccgaagt ggtggtgcaa cgcgccgccg tgcagaccct
     2881 gcttcccaag ggaaccatca agggcctgta agcgtcgccg ccggcgcctg accggccgcg
     2941 cgcactttgc catccgagtt gcctagcatg aaccgatatc cgctctgggt ttacatcacc
     3001 gtcgccgtcg cgctggtggt cggcctgatc tataccctgc ccaacttcta cggcgaatcg
     3061 cccgcggtgc agatttccag cgcccgcgcg acggtcaaga tcgacgaaag cacgctcgaa
     3121 cgcgccgaga aggcgctcgc cgacgcaggc atcgcctacg acggcgcgcg tatggatccg
     3181 acgggcgtga aggtgcgctt cgccgatacg gacacgcagt tgcgcgcccg cgacctgctg
     3241 gatcaggcct tcaatccgga cccgagcgcc ccggaccaca cggttgcgct caacctgctg
     3301 cccgcctcgc ccaactggct gtcggccatc ggcgcgcagc cgatgtatct gggcctggac
     3361 ttgcgcggcg gcgtgcactt cctgctgcag gtggacatgc agggcgcgat catcaagcgt
     3421 ctcgattcct ataccggcga catccgcagc atgctgcgcg acgcccgcgt ccgccacagc
     3481 ggcgtgagcc gcgaagggtc gaccgtggtc atccgcttcc gcgacagcca gacgcgtgcg
     3541 gaagcgtcca aggtgctgac ccgcgacctg cccgatctga tgctgaacga agtgggcacc
     3601 gagtcgcagc cgcgcatcgt agcgaccatc cggcccgaag cgcagaagca gatccaggaa
     3661 tacgcactgc ggcagaacat ccagacgctc cacaaccgga tcaacgaact gggtgtggcc
     3721 gagccggtga tccagcagca gggcgccgac cggatcgtcg tgcagttgcc gggcgtgcag
     3781 gacaccgctc gcgccaagga aatcctcggc cgcaccgcca cgctggaaat ccgcatggtg
     3841 gacgaggaga acatgacgcc cgaccgcctc gccgccgcag cgcggggcga agtgcccttc
     3901 ggcagcgagt actacgtgac gcgcgaaggc cagccgctgc tgatcaagcg gcaggtcgtg
     3961 ctgaccggcg accgcctgac caacgcgcag gccggcttcg acggcatgac caacgagccg
     4021 gcggtgcacc tgacgctgga cgcgacgggc gcgcgcatct tccgcgacgt gacgcgcgag
     4081 aacgtcggcc ggcgcatggc catcctgctg ttcgagaaag gtcgcggcga agtcgtcacc
     4141 gcgccggtca tccgtgacga aatcggcggc ggccgcgtgc agatcagcgg tcagatgaac
     4201 accagcgaag ccaacgacgt ggcgctgctg ctgcgcgccg gttcgctggc cgcgccgatg
     4261 gacatcgtcg aggaacgcac catcggcccg agcctgggta aggacaatat cgaacgcggc
     4321 ttccagtcca cgatgtgggg cttcatcgcc gtggtcgtgt tcatgtgcat gtactacgcg
     4381 ctattcggcg tgttctcgtc catcgctctg gcgttcaacc tgctgttgct gatcgcgctg
     4441 ctgtccatgc tgcaggccac gcttacgctg ccgggcatcg ccgccattgc gctgaccctg
     4501 ggcatggcga tcgacgccaa cgtgctgatc aacgagcgga tccgcgagga actgcgcgcc
     4561 ggcgcgacgc cgcaagcggc gatccatgcc ggttacgaac gcgccttcgg caccattctg
     4621 gactcgaacc tgaccacgat gatcgccgcg cttgccctgc tcattttcgg ctcgggcccc
     4681 gtacgcggct tcgctgtagt gcatgcgctg ggcatcctga cctcgatttt cagttcggtg
     4741 gtcggctcgc gctcgctggt caacctctgg tacggatcgc gacgcaaggt cgagcgcctg
     4801 gcgatcggca atacctcctg gaagtaagca cgccaacgcg acacaaggga agacgatatg
     4861 gagttcttta agatccggcg cgacatcccg ttcatgcgcc atgcgctggc gttcaacatc
     4921 atttcgctgg tgaccttcct gctggccgtg ttcttcatct ggcacaaggg gctgcacttc
     4981 tccatcgagt tcaccggcgg gacggtgatg gaagtcagct attcggaagc ggccgacgtg
     5041 cagcgcatcc gcgaaacact ggaaaacgcc ggctacaacg atccgcaggt acagaacttc
     5101 ggctcctcgc gcgacgtcct gatccgcctg ccgctgcgcg aggatcagtc cagcgcacag
     5161 gtctcgcaag cggtgatgca gaaactgcag gcggacaacg ccggtgcgca gttgcagcgc
     5221 gtggagttcg tcggaccgca ggtgggacgc gaactggcgg agaacggcgc gctcgcgctg
     5281 ctcatcacct gcctgggcat catgatctat ctggccttcc gcttcgaatg gaagttcggc
     5341 ctggcgacag tcatcgccaa cctgcacgac gtggtcatca tcctgggctt cttcgccttc
     5401 ttccagtggg agttttcgct gcccgtgctc gcggcggtgc tggccgtgct gggttattcg
     5461 gtgaacgaat cggtggtcat cgccgaccgt atccgcgaga atttccgcaa aatgcgcaag
     5521 acgccggtga cggacgtgat caacaacgcc atcacccgca ccatttcgcg gaccatcatc
     5581 acccacggca gtacgcagat ggtggtgctg gccatgctgt tcttcggcgg cccggcgctg
     5641 cactacttcg ccatggcgct gaccatcggc atctgcttcg gcatctactc ttcgg
//
LOCUS       DATGOU010000018         3623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3600216, whole genome shotgun sequence.
ACCESSION   DATGOU010000018 DATGOU010000000
VERSION     DATGOU010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3600216"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1159)
                     /locus_tag="VKZ48_00980"
     CDS             complement(182..1159)
                     /locus_tag="VKZ48_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HLU76456.1"
                     /translation="MTALRFLAKCFCSLLVALGPLAAGAQTWPEKPVTIVVPWPPGGP
                     SDIAARPVAKGMQDLLGQTFLVENRGGAGGNLGTAAVTKAAPDGYTLLITSSAPIVIN
                     PSLYRNMPFDPSKDLIPITNVLRVPLVLVAHPSVPANNLKELIAHIKAQKGGFQYASA
                     GNGTPQHMTGELFKSVAKLDVIHVPYKGSAPAINDLLGGHVPVMFDSMVAITPHIKSG
                     KVKAIAMTGAQRSPQFPDLPTFAESGFPEVESYAWYGFFAPAGTPKALIDQINAAALK
                     AMKQPEFQRTLADTGSDFVGDTPENFRKFVEAEAQKWGRVVKESGARID"
     gene            complement(1273..2289)
                     /locus_tag="VKZ48_00985"
     CDS             complement(1273..2289)
                     /locus_tag="VKZ48_00985"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008248910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HLU76457.1"
                     /translation="MPRIRAWAARTPRAPLERYDFAPGPLATEDVEIAVEYCGICHSD
                     LSMLDNEWGMTQYPLVPGHEVIGRVVAIGEDVKGMKVGQRVGVGWNAGSCTQCRSCLS
                     GDQHLCGQVQPTIVGRHGGFADRLRVNWLWAVPMPAALDASSAGPLMCGGLTVFYPLL
                     SANLSPMSRVGVVGIGGLGHMALQFLNAWGCEIVAFTSSAAKREEALALGAHRVVSST
                     DSAAMQALNGSLDLILYTVNVSLDWEALMATLAPKGRLHLVGAVLEPIPVQAMQLISY
                     EQSISGSPTGPRHAMDAMLDFAARHNIAPRVEHFPMSEVNAALRHLREGKPHYRIVLD
                     ADFG"
     gene            complement(2493..3152)
                     /locus_tag="VKZ48_00990"
     CDS             complement(2493..3152)
                     /locus_tag="VKZ48_00990"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLU76458.1"
                     /translation="MRAWSGYDAWMDPNSLAQITARTLQHYGERADAFWAGTHDHDVS
                     QNIDALLEHIEGDPPYRILDFGCGPGRDLKTLTERGHVAVGLDGAARFCEMARAYSGC
                     EVLHQDFLALHLPAGRFDGIFANASLFHVPHAELPRVLGELRAALKPRGILFSSNPRG
                     ADEEGWNQGRYGTYHRPESWLRHLREAGFEVVHSYYRPSGLPLAQQPWFATVARAPTG
                     D"
     gene            complement(3174..>3623)
                     /locus_tag="VKZ48_00995"
     CDS             complement(3174..>3623)
                     /locus_tag="VKZ48_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76459.1"
                     /translation="GYFKRDTPTNDARKAEGWLAGGGFVADLNARAQVGVEYMVQTED
                     AREDFESRTSHGPIIYGTWHVHPLLSLNASYRYSDVKYDEIQGIFPEKREDDQHVLDF
                     SALWDVSRYVYPGTAVRLQHTYIRGNSNLELYDNRRHITTIGMQVVF"
BASE COUNT          651 a         1189 c         1168 g          615 t
ORIGIN      
        1 acttcaagtg catgaacgat ctggagccgc agcgcgtcat ggccgagatc gagaagctgg
       61 aaacgacggc ggcctgaaac ttaaaagctt gaaacacaga gatcactgag gaccacggag
      121 cacacggaga aaagcaacaa ccactcagtg ctccccgtgc tctccgtgtt tcagccttcc
      181 gtcaatcgat ccgcgcgccg ctctccttca ccaccctgcc ccacttctgc gcttccgctt
      241 cgacgaattt gcggaagttc tccggcgtat cgccgacgaa gtcggaaccg gtgtcggcca
      301 gcgtacgctg gaattccggc tgtttcatcg ccttcaacgc cgcggcgttg atctggtcga
      361 tgagcgcctt gggcgtgccg gccggcgcga agaaaccgta ccacgcatag gactccactt
      421 ccgggaagcc cgattcagcg aaggtcggca gatcggggaa ctgcggcgaa cgctgcgcac
      481 cggtcatggc gatggccttc acctttcccg atttgatgtg cggcgtgatg gcgaccatgc
      541 tgtcgaacat caccggcacg tggccgccga gcagatcgtt gatcgccggc gccgagccct
      601 tgtagggcac gtggatgacg tcgagcttgg ccaccgactt gaacaactcg ccggtcatgt
      661 gctgcggggt accgttaccg gccgatgcgt attgaaagcc gcccttctgc gccttgatgt
      721 gcgcgatcag ttccttgagg ttgttcgccg gcaccgaagg atgcgccacc agcaccagcg
      781 gcacacgcag cacgttggtg atcggaatca gatccttcga cggatcgaag ggcatgttgc
      841 gatagaggct cgggttgatg acgatcggcg cgctggaagt aatcaacagc gtgtagccgt
      901 cgggcgccgc cttggtcacc gccgcggtgc ccagattgcc gcccgcgccg ccgcggttct
      961 caaccaggaa ggtctggccg agcagatcct gcatgccctt cgccaccggc cgcgcggcaa
     1021 tgtcggacgg cccgccgggc ggccagggca cgacaatggt gaccggcttt tcgggccagg
     1081 tctgcgcacc ggccgcgagc ggacccaagg cgaccagcag gctgcaaaaa cacttcgcca
     1141 aaaaacgcaa tgcagtcatg aaacacctcc tcgatgggtg cggtctcgcg tcctccgggc
     1201 agcgtacgtt cccgggaccg tgccggatga tatgccgggc gggtcgggcc gcattgcgcc
     1261 ggcgacgaac ggtcagccga aatccgcatc cagcacgatg cgatagtgcg gcttgccttc
     1321 gcgcaggtgc cgcaacgcgg cgttcacctc gctcatggga aagtgctcca cgcgcggcgc
     1381 gatgttgtgc cgcgcggcga aatcgagcat cgcgtccatg gcgtgacgcg gcccggtggg
     1441 cgacccgctg atgctctgct cgtaggaaat cagttgcatc gcctggacgg gaatcggctc
     1501 cagcaccgcg ccgaccagat gcagccgccc cttgggcgcg agcgtcgcca tcagcgcctc
     1561 ccagtcgagg gaaacgttga cggtatagag aatgagatcg agcgagccgt tgagcgcttg
     1621 catcgccgcg ctgtcggtac tcgacacgac ccggtgcgca cccagggcga gcgcttcctc
     1681 acgcttggcg gcgctggagg taaaggcgac gatctcgcag ccccacgcat tgagaaattg
     1741 cagcgccatg tggccgagcc cgccgatgcc gaccacgccc acgcgcgaca tgggcgacag
     1801 attggcgctc aggagcggat agaacactgt cagtccgccg cacatcagcg gcccggcgct
     1861 gctcgcatcc agcgcggcgg gcatgggcac ggcccacagc cagttgacgc gcaagcggtc
     1921 ggcgaagccg ccgtgacgcc cgacgatggt cggctgcacc tgtccgcaca agtgctggtc
     1981 gccgctcagg caactgcggc actgcgtgca actgccggca ttccagccga cgccgacgcg
     2041 ctgccccact ttcatgccct tcacgtcctc gccgatggcg accacccggc cgatcacttc
     2101 gtgaccgggc accagcgggt actgcgtcat gccccattcg ttgtccagca tggacagatc
     2161 ggaatggcag atgccgcaat actcgaccgc gatttctacg tcctcggtcg ccagcggacc
     2221 gggcgcgaaa tcgtagcgtt ccagcggtgc gcgaggtgtg cgggcggccc aggctcggat
     2281 gcgtggcatg aacgtgactc ctctcagtag acgctgattc cagcttatcg gccgcgcccg
     2341 cgcaagcgga catccagtgt tttcaaccgg ctaccgggct tccttgcgcg gattcgaacc
     2401 gatcaagccg cagcgccagg gctatcgcaa tgcccatgcc cgcacgccaa tgggtcttca
     2461 gcgtccggcc ccggatgcga cccggccggc gctcaatcac ccgtcggtgc gcgcgccacc
     2521 gtggcgaacc agggctgctg cgcgagcggc aaaccggacg ggcggtaata actgtgcacg
     2581 acttcgaagc cggcctcgcg caaatggcgc agccagcttt ccggccggtg atacgtgccg
     2641 tagcggcctt ggttccagcc ctcttcatcc gcaccgcgcg gattggaact gaagaggatg
     2701 ccgcgcggct tcagtgcggc gcgcagctcg ccgagcacgc gcggcagttc cgcgtgcggc
     2761 acatggaaca gggaggcatt ggcaaagatg ccgtcgaagc gtccggcggg caggtgaagc
     2821 gcaagaaaat cctgatgcag gacttcacag ccgctgtagg cgcgggccat ctcacagaag
     2881 cgcgccgcgc catccaaccc gaccgcgaca tggccgcgct cggtgagcgt tttgagatcg
     2941 cgccccgggc cgcaaccgaa atcgagaatg cggtacggcg ggtcgccctc gatgtgctcg
     3001 agcagcgcgt cgatgttctg cgagacgtcg tgatcgtgcg tgccggccca gaaggcgtcg
     3061 gcgcgttcgc cgtagtgctg cagcgtacgg gcggtgattt gggcgagcga gttgggatcc
     3121 atccaggcat cgtagccgct ccatgcccgc atgcaaacct gcacgcgcgg cggtcagaac
     3181 accacttgca tcccgatggt ggtgatgtgc cggcggttgt cgtacaactc gagattcgag
     3241 ttgccgcgga tataggtgtg ctgcaggcgc accgccgtgc ccggatagac gtagcggctt
     3301 acgtcccaca gcgcactgaa gtcgagcaca tgctggtcgt cctcgcgctt ctcgggaaag
     3361 atgccctgaa tctcgtcgta cttcacgtcg ctgtagcggt agctggcgtt caggctcagc
     3421 agcggatgaa catgccaggt gccgtaaatg atcggcccgt gcgaagtgcg cgattcgaaa
     3481 tcctcgcgcg catcttcggt ctgcaccatg tattccacgc ccacctgcgc gcgggcattg
     3541 aggtcggcca cgaagccccc gccggcgagc cagccttcgg ccttgcgcgc gtcgttggtc
     3601 ggcgtgtcgc gcttgaagta gcc
//
LOCUS       DATGOU010000019         4276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_1381138, whole genome shotgun sequence.
ACCESSION   DATGOU010000019 DATGOU010000000
VERSION     DATGOU010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381138"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VKZ48_01000"
     CDS             <1..510
                     /locus_tag="VKZ48_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HLU76460.1"
                     /translation="QGDTVLKPLDGMGGTGVFRVTRQDMNRNAIVETLSHDGRRTVMA
                     QRFIPEISAGDKRILLIDGEPVPYVLARIPKPGETRGNLAAGGTGIAQPLSDHDRAVA
                     EAIGPTLAQRGLLLVGLDMIGDYITEINVTSPTCFREIHDQTGFDVARMYVDALERAI
                     DARSRREGV"
     gene            573..986
                     /locus_tag="VKZ48_01005"
     CDS             573..986
                     /locus_tag="VKZ48_01005"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HLU76461.1"
                     /translation="MIGILIISHGAFGEALIHCASHVLGKRPLRVRQVGITVHDDPEA
                     ILPIARDMARQLDEGQGVLVLTDMVGATPANIATKLYEPGKVAVVSGANLPMLVRALT
                     YRNEALNTVAAKAMSGGHDGIVHLPPQGPNAATGN"
     gene            967..1236
                     /locus_tag="VKZ48_01010"
     CDS             967..1236
                     /locus_tag="VKZ48_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HLU76462.1"
                     /translation="MPQREIEIVNKLGLHARASAKLTQTASRFKSDIWLSRNGRRVNA
                     KSIMGVMMLAAGKGSRIEIDAVGEDADAALGALVALIGDKFGEGE"
     gene            1247..3022
                     /gene="ptsP"
                     /locus_tag="VKZ48_01015"
     CDS             1247..3022
                     /gene="ptsP"
                     /locus_tag="VKZ48_01015"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HLU76463.1"
                     /translation="MGKPDLEAEQALLTPCEGASFALHGAGASAGIAIGHAHLVTSAR
                     MEAPRYEIDAADIDAELTRFDRAIRQVREELLSLHKAVPDNAPPEMAAFLDLHRMVLD
                     DSMLSQTPRELIRSKRYNAEWALIQQTEALVQRFEEVEDAYLRERGADVQQVAERILK
                     ALMGSRHAPPLAGEAGEIIVVAHDFSPADMILFRQHNFAGFVTDLGGVTSHTAIVARS
                     LNLPAVVGMHHARQMVREHELLIVDGAAGVLIVNPDPQVLGEYRLRQRAFELERQKLR
                     RLRRTPASTLEGVAVELHANIELPQDVKEALGVGAAGVGLFRSEFLFLNRDDFPSEDE
                     QFEAYRSVISAMRGLPVTIRTLDIGADKTLDGHHSGGPNPALGLRAIRYCLAEPQMFM
                     TQLRAILRASHYGKVRILLPMIGHFHEVEQAAQILAQARRSLAEQGIPFDPAIPVGGM
                     IEVPAAALAAAQFVKRLDFLSIGTNDLIQYTLAIDRTDDKVAHLYDPLHPAVLQLIAQ
                     TIRTGARAGVPVAVCGEMAGEVVYARLLLGLGLRQFSMHPSQLPAVKQQILRSSVPVA
                     ETLAQRIIKSDDPEKSRQLLARLNA"
     gene            3052..3441
                     /locus_tag="VKZ48_01020"
     CDS             3052..3441
                     /locus_tag="VKZ48_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76464.1"
                     /translation="MLREIEGLRQDEPALRRRWFHDDDFDLFVWQHEDGRVTAFELCY
                     GASGSECALVWRQGEGFYHDGPPPQTAVGASGNDVFRERFTAAAVQLPLSLRDTLDQR
                     LAEYLATRDRVTTRRKRFRRADWQEAG"
     gene            3669..>4276
                     /locus_tag="VKZ48_01025"
     CDS             3669..>4276
                     /locus_tag="VKZ48_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006761998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLU76465.1"
                     /translation="MNEPRSVGPVSASRIHFNQPIRLKSGATFGQYDLVYETYGTLNG
                     ARSNAVLICHALNASHHVAGFYADDPKNIGWWDNMVGPGKPVDTDRFFVIGVNNLGGC
                     FGSTGPASIDPETGKPYGSSFPVLTVEDWVQVQARLADHLGIERFAAVMGGSLGAMQA
                     LQWTISFPERVAHSLVIAAAPKLSAQNIAFNEVARQAIVTDP"
BASE COUNT          777 a         1442 c         1379 g          678 t
ORIGIN      
        1 cagggcgata cggtgctcaa gccgctcgac ggcatgggcg gcaccggcgt cttccgggtg
       61 acccgccagg atatgaatcg caacgccatc gtcgagacct tgtcgcacga cggccggcgc
      121 acggtgatgg cgcagcgctt cattccggaa atcagcgccg gcgacaagcg catcctgctg
      181 atcgacggcg aaccagtgcc ttatgtactg gcgcgcatcc ccaagccggg cgagacacgc
      241 ggcaatctgg cggctggcgg caccggcatc gcgcagccgc tgagcgacca cgaccgcgcc
      301 gtcgccgaag ccatcgggcc gacgctggcg cagcgcggcc tgctgctggt cggactcgac
      361 atgatcggcg actacattac cgaaatcaac gtcaccagcc ccacctgctt ccgcgaaatc
      421 cacgaccaga ccggcttcga tgtggcgcgg atgtacgtcg acgccctgga gcgggcgatt
      481 gacgcgcgca gcaggcgcga aggcgtttag actaagcgtt gtccgagcgg tccgccggac
      541 gcggaccgca aaaccattcc gaccccgcga acatgatcgg catcctgatc atcagccacg
      601 gcgccttcgg cgaagcgctc atccattgcg cgagtcatgt gctcggcaaa cgcccgctgc
      661 gcgtgcggca ggtgggcatc accgtgcacg acgatcccga ggcgatcctc cccatcgccc
      721 gcgatatggc gcgacaactg gacgaaggcc agggcgtgct ggtgctgacc gacatggtcg
      781 gcgccacacc ggccaacatc gccaccaagc tctacgaacc gggcaaggtg gccgtcgttt
      841 ccggcgcgaa cctgcccatg ctggtacgcg cactgaccta ccgcaacgaa gcgttgaaca
      901 ctgtggcggc caaagcgatg agcggcggcc acgacggcat cgttcatctc ccgcctcaag
      961 gaccgaatgc cgcaacggga aattgaaatc gtcaacaagc tgggcctgca cgcgcgcgcc
     1021 tcggccaaac tgacccagac cgccagccga ttcaagtcgg acatctggct gtcgcgcaac
     1081 ggccggcgcg tcaacgccaa gagcatcatg ggagtaatga tgctggccgc cggaaagggc
     1141 agccgcatcg agatcgacgc cgtcggcgag gatgccgatg cggcgctggg cgcgctggtc
     1201 gcgctgatcg gcgacaagtt cggcgaaggc gaatagggaa gcgcacgtgg gcaagcccga
     1261 tctcgaagcg gagcaggcat tgctgacgcc ctgcgaaggc gccagtttcg cgctgcacgg
     1321 cgcgggagcc tcggcgggca tcgccatcgg ccatgcgcat ctggtcactt cggcacggat
     1381 ggaagcgccg cgctacgaaa tcgacgcggc cgacattgac gccgaactga cccgtttcga
     1441 tcgcgccatc agacaggtgc gggaggaact gctgtcgctg cacaaggcgg tacccgacaa
     1501 cgcgccgccc gagatggccg ccttccttga tctgcatcgc atggtgctgg acgactccat
     1561 gctctcgcag acgccgcgcg aactcatccg cagcaagcgc tacaacgccg aatgggcgct
     1621 catccagcag accgaggcgc tggtgcagcg cttcgaggaa gtggaggacg cctatctgcg
     1681 cgaacgcggc gcggacgtgc agcaggtggc cgaacgcatc ctcaaggcac tgatgggcag
     1741 ccggcatgcg ccgccgctcg ccggcgaagc cggcgagatc atcgtcgtcg cgcacgactt
     1801 ttcgccggcc gacatgatcc tgttccggca gcacaacttc gccggctttg tcaccgatct
     1861 gggcggcgtc acctcgcaca cggccattgt ggcgcgcagc ctgaatctgc ccgcggtggt
     1921 cggtatgcat cacgcgcggc agatggtccg cgagcacgaa ctgctgatcg tggacggtgc
     1981 ggccggcgtg ctgatcgtca atcccgatcc gcaggtgctg ggcgaatacc ggctgcggca
     2041 acgcgccttc gaactggaac ggcagaagct gcgtcggctg cgccgcacgc ctgcaagcac
     2101 gctcgaaggc gttgcggtgg agttgcacgc caacatcgag ctgccgcagg atgtgaagga
     2161 agcgctcggc gtcggcgccg cgggcgtcgg tctgttccgc agcgagttcc tgttcctcaa
     2221 tcgcgacgat ttccccagcg aggacgaaca gttcgaggcc taccgctcgg tgatctccgc
     2281 gatgcgcgga ctgccggtga ccatccgcac gctggacatc ggcgcggaca agacgctcga
     2341 cggccaccac agcggcggcc ccaatccggc gctcggcctg cgcgccatcc gctactgtct
     2401 ggccgagccg cagatgttca tgacccagct gcgcgccatc ctgcgcgcct cgcattacgg
     2461 gaaggtgcgc atcctgctgc ccatgatcgg gcacttccac gaggtcgagc aggcggcgca
     2521 gatcctggcg caggcgcgcc gctctctcgc cgagcagggc atccccttcg atcccgcgat
     2581 tccggtcggc ggcatgatcg aggtgccggc cgccgcgctg gcggcggcgc agttcgtcaa
     2641 gcggctggac ttcctctcca tcggcaccaa cgacctgatc caatacacgc tggccatcga
     2701 ccgcaccgac gacaaggtgg cgcatcttta cgacccgctg catccggccg tgctgcaact
     2761 catcgcccag accattcgca ccggcgcgcg cgccggcgtg ccggtggcgg tgtgcggcga
     2821 aatggccggg gaagtggtct acgcacgttt gttgctgggc ctgggattgc ggcagttttc
     2881 catgcacccg tcgcaactgc ccgcagtgaa gcaacagatc ctgcgctcca gcgtcccggt
     2941 cgccgaaacg ctggcgcagc gaatcatcaa aagcgacgat ccggaaaaat cgcgccagct
     3001 gctggctcgc ctgaatgcct gagcccgccc gtgggcgcag gaggaggcgt catgctgcga
     3061 gagattgaag ggctgcgtca ggacgagccg gcattgcgcc gtcgctggtt tcacgacgac
     3121 gatttcgatc tcttcgtctg gcagcacgag gacggcaggg tgaccgcctt cgagttgtgc
     3181 tacggggcaa gcggcagcga gtgcgcgctg gtctggcgcc aaggcgaagg tttctatcac
     3241 gatggcccgc cgccgcagac ggccgtgggc gcctcgggca acgatgtctt tcgcgaacgc
     3301 ttcactgccg ctgccgtgca actgcctctg agtctgcgcg atacgctcga tcagcggctg
     3361 gccgagtacc tcgccacgcg cgaccgcgtg accacccgcc gcaagcgctt tcgccgggcg
     3421 gactggcaag aggccggtta atcgcgctaa gattcgtccc tcgggcggat gacggtcgga
     3481 tcgtcgtctt ccttcagcgc cgatttgaga tcagcttgcg ggcgcccgcc gtacggacgg
     3541 cggacaaaaa agtacggcta aagcgagggc aacccgttta gagccgcgca agctcaaacg
     3601 ggtttttttg tagtaatcgc tccgcagatg gagattgcgc cgcgcgcccc acgcacgcgt
     3661 agaacaagat gaacgaacca cgctcggtcg ggccggtcag cgcctcgcgc atccacttca
     3721 atcagcccat ccgcctcaaa agcggcgcca ccttcggtca gtacgacctc gtctacgaaa
     3781 cctacggcac cctgaacggc gcgcgcagca acgcggtgct gatttgtcac gcgctcaacg
     3841 cctcgcatca cgtcgccggc ttctacgccg acgaccccaa gaacatcggc tggtgggaca
     3901 acatggtcgg accgggcaag ccggtggata ccgatcgctt cttcgtcatc ggcgtgaaca
     3961 atctgggcgg ctgtttcggt tccaccggtc cggcatcgat cgatcccgag accggcaaac
     4021 cctacggctc cagcttcccc gtgctgacgg tggaggattg ggtgcaggta caggcacgcc
     4081 tggccgatca tctgggcatc gagcgcttcg ctgccgtcat gggcgggagc ctgggcgcca
     4141 tgcaggcgct gcaatggacc atctcgtttc ccgagcgggt cgcacattcg ctcgtgatcg
     4201 ccgcggcccc caagctttcg gcgcagaaca tcgcattcaa tgaagtcgcg cgccaggcca
     4261 tcgtgaccga cccgga
//
LOCUS       DATGOU010000020        46806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_5825249, whole genome shotgun sequence.
ACCESSION   DATGOU010000020 DATGOU010000000
VERSION     DATGOU010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 46806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46806
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5825249"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VKZ48_01030"
     CDS             complement(<1..415)
                     /locus_tag="VKZ48_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HLU76466.1"
                     /translation="MNARTELSVLPAVEVPLPRFVQIEPVGQCNLRCAMCAIQFREDV
                     PQNRRGAFLPFAEFRRLLDQFGDVGELHLQGLGEPLMHPDFFRMVREAVSRGLKVSTN
                     SNLTLLTEARARECVDSGLDTLHISLDGASATVYES"
     gene            complement(412..1395)
                     /locus_tag="VKZ48_01035"
     CDS             complement(412..1395)
                     /locus_tag="VKZ48_01035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLU76467.1"
                     /translation="MTPAPLVSVVVPTCGRPTLLQRCLQALVRQTLSGDAYEIVVTDD
                     GHSDETRALVARIDAANPGGPRVRYVRSQAPRSGPATARNAGWHAAHAPVIAFTDDDT
                     IPSREWLREGLRAVQPGVAAVTGRTHVPLPPAPTDWERNTAGLERGEFITANCFVRRE
                     VLEDAGGFDERYRRPWREDSDLHFTILERGLRIEQAPLALVIHPVREAPWGVSARIQQ
                     NVYFDALLFKKHPQLYRTRISSGPPLRYYFAVGAFAVGIIGAIGGMPFVAAGGLGAWL
                     LWTLGFTAYRLRGASLSPRHIAEMLYTSAVIPPLALYWRIAGALRFKVAFA"
     gene            complement(1392..3164)
                     /locus_tag="VKZ48_01040"
     CDS             complement(1392..3164)
                     /locus_tag="VKZ48_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014973094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HLU76468.1"
                     /translation="MYTLGINAAFHDSAACLVSDGVVIAAAEEERFSRIKHAKRPVPF
                     TVWELPYGAIDYCLREAGITLADVDHVAYSLDPKLLPGRPADDAPIVFAHEPSAAPRN
                     DKGPWDDLFYLYALNAPRQLAGGAPHHLRKRFDTGKPHKYQWHFVEHHLAHEASAFLA
                     APFESCAVLTLDGRGERATTTHGVWRDGALQRLKEVEVPHSLGLLYEDTTRYLGFLHS
                     SDEYKVMALASYGTPRHLDAFRDIVQLDGAGGYRITGPDLEKRFGPARECNASFEAHH
                     FDIAASLQKVLEESALSLARWLRKASGERQLVMAGGVALNCVMNARIRDAGIYEDIWV
                     QPAAGDAGTALGAALWIDYVQRGTRNWHMDHAYYGPAYDDEEIEQLLEWSKIPYRRLD
                     DVAEETAKLLAQNYIIGWFQGRMEFGPRALGARSILASPIEPEMKARLNELKDREDFR
                     PVAPVVLEERLDDWFVREPDVTGKAPFMLFVYGVREDIAQRIPAAVHVDRTARVQTVD
                     GQSNPRYRALLEAFEQRTGVPVLVNTSFNSRGQPIVCSPRDAIEAFYSTPLDALVIGS
                     FLLTKQITAQGDPLLAASMVEAIV"
     gene            complement(3152..4324)
                     /locus_tag="VKZ48_01045"
     CDS             complement(3152..4324)
                     /locus_tag="VKZ48_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HLU76469.1"
                     /translation="MRSHADAVPLHAATALWRRARRILCVRLDALGDVLMTTPALRAL
                     KHSAPAREIFLLTSPAGAAVAPHVPEIDRVLVWEAPWIKHPQAPTPAATDAFVERLRS
                     ERYDAAVIFTVYSQSALPAAMLCWLADIPLRLAHTRENPYHLLSDWEPEREPAALLRH
                     EVRRQLDLVASIGARTNDERLSFKLYEKDDARALRALRKAGVRSTRDWIVVHPGASAA
                     SRRYPLEHYAAALRLLAQADDRPILVTGGAQERDLVHALAAEVPGAVPLAGALRLGEL
                     GCVISRAALLIANNTGPAHIAAALGTPIVDLYALTNPQHTPWQVPHRVLSHDVPCRNC
                     YRSVCPERHHLCLRGVPPEAVVRAALELLQEREPHASPPTLHAHTERATEDALCIR"
     gene            complement(4324..4860)
                     /locus_tag="VKZ48_01050"
     CDS             complement(4324..4860)
                     /locus_tag="VKZ48_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013341531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HLU76470.1"
                     /translation="MKPAVFIDKDGTLVHDVPYNVDPSRIAVREEAGEALRRLQRAGF
                     ELVLVSNQAGVALGHFDEDALDEVWSHLRERLGQDGVDLSAFYYCPHPPGGGCDCRKP
                     KPGMLQRAAQERDIDLARSWMLGDILDDVEAGHRAGCRSVLLDVGSETEWRESELRTP
                     AFLARDLERAAHFILASS"
     gene            complement(4872..5603)
                     /locus_tag="VKZ48_01055"
     CDS             complement(4872..5603)
                     /locus_tag="VKZ48_01055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLU76471.1"
                     /translation="MTTLKDKLVIVTGGARGLGAALCERLAQEGSKVVCVDLKLELAQ
                     ETCARIRDKHRDAVNWACPADIGDAQQVETLVREVTERYGPIDIVINNAGVDITLSID
                     ELAVDDFDRVVQTNLRGPFLLSKYVAPHMRERGGGHIVNIASTAAKRAWPNASAYHAS
                     KWGLLGLSHALHAELRPQGIKVTAVVAGGMRTPFLFDRFPDIDPEVLQEPADVAEAIA
                     GVLKVGGATVIPEVMILPMRETSWP"
     gene            complement(5600..6874)
                     /locus_tag="VKZ48_01060"
     CDS             complement(5600..6874)
                     /locus_tag="VKZ48_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HLU76472.1"
                     /translation="MKIALISEHASPLATLGGVDSGGQNVYVASIARELARAGHTVDV
                     YTRCDDDSLPGIVPFAPGVRVLHIPAGPRRYVPKEDLLPYMQEFALRMANYCTPSSRR
                     YDVVHANFFMSGIVAARLQEELGMPFVMTFHALGKVRRLHQKDADRFPAVRTSIEEQL
                     VQRADRIIAECPQDRDDLIDLYDADPARIDVIGCGVDIEELHNAGCGVRTVLGVSDDE
                     LLVLQLGRMVPRKGVDNVIRAIGCLKRDHGIRAQLLVVGGESSAPDPVLTPEIGRLMA
                     VADQEDVRALVSFVGQRPRQMLRHYYSSADVFVTTPWYEPFGITPLEAMACGTPVIGS
                     NVGGIKHTVLDEVTGYLVPPNDPGALAERLARFHRNPELARAFGRAGIRHVRTHYTWR
                     KIAEQVADVYAATAQPARVRRISTTQETVATL"
     gene            7259..8113
                     /locus_tag="VKZ48_01065"
     CDS             7259..8113
                     /locus_tag="VKZ48_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLU76473.1"
                     /translation="MSAQQRPPQHQDQQPGREAPMQPQTQSFMRDYVGAGRLKGQAVL
                     ITGADSGIGRAVAIGSAKEGADIVIAYLEEHEDAEATREAVEREGVHCLLIPGDIAVE
                     DHCRYVVERTVKDLGRLDVLVNNAAEQHPQERPEQISATQLQRTFATNVFAMFHLVNA
                     ALPHLQSGARIINSASVTAYKGNPKLIDYASSKGAIVAFTRSLALALAGRGIRVNAVA
                     PGPVWTPLIPSTFPAEDVATFGSDVPLGRAGQPDDIAPCYLFLASADSTYMTGQVLHP
                     NGGSIING"
     gene            8393..9781
                     /locus_tag="VKZ48_01070"
     CDS             8393..9781
                     /locus_tag="VKZ48_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLU76474.1"
                     /translation="MPHVLMVDDDPEALEWLSEFVKSEGFTVAKASSLRAARINLAQI
                     RPDVVFADLQLPDGEGIELIQDLETLEGTEFVLITGHATVESAINALRQGASDYLTKP
                     VDIDRLRAILKRVPGPAELREEIGVLRQELRGLGRFGHILGNSPAMQKLYDQIGRVAP
                     TSATVLLIGESGTGKELAAQTVHELSRRKKRPFLPLNCGAVSPNLIESELFGHEKGSF
                     TGADRQHKGFFERADGGTLFLDEITEMPLELQVKLLRVLETGSLMRVGMNTPIPVDVR
                     VIAATNRNPAKAVQEGKLREDLYHRLNVFPITLPPLRERATDIEFLAEHFLDVLNQEE
                     GTNKEFSPQAIAALYAHNWPGNVRELKNYVQRVYILADRVIDVEFAPRMFAEPEPKPI
                     LTVRVGSTLEEVNRRLIEATMAECGNVRRKAAETLGISLKTLYNRLAQYNAEKGGQEM
                     QPGDEEEEYEKS"
     gene            complement(9845..9982)
                     /locus_tag="VKZ48_01075"
     CDS             complement(9845..9982)
                     /locus_tag="VKZ48_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76475.1"
                     /translation="MDRATALAYMSAVTDYQVSQVVDKTKGIHAMIDKSHFLQSKRAP
                     K"
     gene            10061..10534
                     /locus_tag="VKZ48_01080"
     CDS             10061..10534
                     /locus_tag="VKZ48_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76476.1"
                     /translation="MYRPFITRETAQQAVGAVRPLFERAVGTPDFGDSGCAHVVVMDP
                     HARVGVVAFEQAILYEESFNRERWDADYAMYARAKARVSWRTGRDSDGLSIAAPQLLE
                     TGDTLLGGAICADGIVVAVSGMYAWYDEALAICVAAFLRAVLKGRLAACDKAFIE"
     gene            complement(10562..11152)
                     /locus_tag="VKZ48_01085"
     CDS             complement(10562..11152)
                     /locus_tag="VKZ48_01085"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HLU76477.1"
                     /translation="MPTRQRPQPPNALTMLTADHRAVQKLFKAFDKRDRGDEASGVEI
                     VETACVELKVHAIIEEEIFYPAVRSKLGDEGESLLLEAAVEHEVAELLIDRLGELEPG
                     EPEYAATFAVLAEYVQQHIKDEEKQLFPRVRKLNELDLEELGAEMQARKDELLSEIEM
                     DVALPGDEETHDGEETLARTPADSGEAFAKRFLPER"
     gene            11276..11611
                     /locus_tag="VKZ48_01090"
     CDS             11276..11611
                     /locus_tag="VKZ48_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76478.1"
                     /translation="MAKKDPTKVPSPKGVVDPEALARQQNDIEKEGGMRRHVSDHDLR
                     QPNERDTAPDGGMRGTDRATDLPQKDMPRAHEDIERGVRDTDRGTPSNVPSSADNRRA
                     TEKQGAKGG"
     gene            11683..11982
                     /locus_tag="VKZ48_01095"
     CDS             11683..11982
                     /locus_tag="VKZ48_01095"
                     /inference="COORDINATES: protein motif:HMM:NF039524.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76479.1"
                     /translation="MQTSDMQGGTGGSFDTEAMRERASANIERASSSAHQTVDKLASQ
                     AQAAKERLAASGEQWAEQGQQYLEQTRDMVRANPLAAIGIAVAAGILLSKLTSRH"
     gene            11989..12465
                     /locus_tag="VKZ48_01100"
     CDS             11989..12465
                     /locus_tag="VKZ48_01100"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HLU76480.1"
                     /translation="MAASAEELTRQHQAALEAASPEYRARFDAARNEPLEGRFDYLWR
                     TTRRVAHGYALLAALDMRRATIQLAWLVGSGVMIAVLGITAWLTLVTALVTWLLQENV
                     SLPLVLVIAAVVNLVGAGFIVWRMRTLVSEMPFAATLRQLKGEAPETERTPGETGA"
     gene            12462..12926
                     /locus_tag="VKZ48_01105"
     CDS             12462..12926
                     /locus_tag="VKZ48_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76481.1"
                     /translation="MRWKPSSLDDEIHAVERCIASDRRLLEQAFVGYVDGVRDSALHT
                     MASPKFLLGALGVGFVIGKFLFRPRKASRQNEATAKRSVLGLLGAGTLSLVQAQFGGP
                     LGLARWLAKTYASRRPTQRMPAAPPQPAPVVHAQPAVVATPAGEEPAVLLRR"
     gene            complement(13016..15001)
                     /locus_tag="VKZ48_01110"
     CDS             complement(13016..15001)
                     /locus_tag="VKZ48_01110"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HLU76482.1"
                     /translation="MIKRILIGAALTLFVLVAALLLVVQSAWVRGIAERQIEKLTGRE
                     VSIGRLDIDLRRCLRIEADEVRIGNPDWAEREHLLNARHVSACLAWLPLLTGTARVKS
                     AALIDAELNLERDSQGRATWAIGAEQKEEKDEPPPIINNVRLSNVLIHFRDAGLGAEL
                     QLRAEGGTPEQPDVAVRAQGRVHETDVHAVLHSPAALPSPEAPVMVSLALTLDRTTAA
                     VAGRLTSLGIDGLDMQLSIAGDSLAALNKLGKIELPDTPPYALRGRLRNRARDHWQVD
                     GLAGRYGDSDVAGHFALELRETRPFFSGEFDVKLLDLDDVLPLVGAAPKTGPGETASA
                     AQRAKAQTQKRDDTIFPDKPLSFTALDILDADVRFRAEQVRRPDAVPINALAGHLTVD
                     NGTVRVQPLDFEVAGGKVATALLLEGRAKPPRASASMDVRQVNLAKLLPDLQKEDVAV
                     GTLYGRGRIEGHGASVAKLLGSADGDVTLMVDGGEMSALVLEAAGLDIAEVVKFLTIK
                     GKPVPLRCAILDLHIENGLAQSELFVLDTDDTVFIGEGHVNFANEEIDVTIHPRPKDE
                     SILALRTPLHLSGTLADPGAGIKGGKLAAKGVAAVALGLVNPLLAILPLIETGPGQDS
                     DCGQFSRRAQTEPKKNAPAENEREKARAAGQEQRSKD"
     gene            15196..18531
                     /locus_tag="VKZ48_01115"
     CDS             15196..18531
                     /locus_tag="VKZ48_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLU76483.1"
                     /translation="MAGPTTELLALSADPADRQRLAERLQRAANAALAFAVFVIALPV
                     IGWIANVDELRRLIPGWPPMHPLMAVFGIGGAATLRLLARSSPKARHAAFAVCASMLA
                     IALAVVWLESDRVWAYEISPYAALTASAVLFAIGCKFWSNYWVRRTADFFAGVAGALA
                     IAGLVGVTYRLMIEMPALVELSLAGGLSVLALAFAVFAGRPDEWLLDQLGDRRVGAIV
                     LRRSLPVVVVFPILLGLARMAAQRAGWLDTAAGGLILTVLTVLGLAAFVLWLAHVLNR
                     VDAERRSAEREAETQREWLRVTLGSIYDAVIATDADCVVRVVNDAAARLLGQPIDGLL
                     RESLAGRVAIHDENGPIDCPLSAVLRGEVGARDYRDLQLELPGDKRLPIEVSVAPIHD
                     GEGGISGGVMVIRDVSERRKHVQVLREAYAELDRRVAERTSALQQANAALHETLALFR
                     GVAESTPDLIFVKNLQQCMVMANPATCRLFGRTEQELIGQRIADLIDDEDIARGVDEH
                     DKRVLHSGRIERVEQVLKTPEGYRTFLSTKSPLRDVNGSIIGLITVATDITERKRIEN
                     DLREAQRFTQGLLDTAPLVLYLLDLDHGRIVFASGMVLQALGYEAEALRAMSTHGLLE
                     LVHRDDRHSLMKHLRDYRDGEDGLRTVELRFRHHDGDWRWLYCRERLFDPVARTRLVL
                     GVAIDVTDRRQAERELEQLIDTEKRLRHEAERANRAKDEFLAVVSHELRSPLNALRGW
                     SFLLGNSNPVDANLLERAVQAIKRNVDHQARLIDDLLDTSRIMSGKLNLEHRPLNLIE
                     TVNAAIEVVRPAAAAKRIDVVFDHPPGALSVEGDPARLHQVVMNLLSNAVKFTPEEGH
                     IGIVAEVTPDDMARIAVTDTGVGIDAQFLPRLFQRFTQADSSTTRKHGGLGIGLALVR
                     HLIELHGGCVTAESEGLNKGATFTVLLPLPQVRVATESLPSGAAAEGSDAPLAGVQIC
                     ALDDDPDARDIIAHALRRAGATVHSASSGAELIALLDEQLPDTRPDVLLLDLAMPGED
                     GFGVLANVRALEKRKGVSEDDAIPAIAVTAFAELSRVRIVEAGFIERVAKPFDPGLLV
                     NTIRRVLAGHRSDAAPLRNRMAG"
     gene            18598..18867
                     /locus_tag="VKZ48_01120"
     CDS             18598..18867
                     /locus_tag="VKZ48_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76484.1"
                     /translation="MRLFLLLALCLHAAPVAADEKVQNAEGVVAPSGQAGEQAERSAA
                     RKADADWIKRCDKLAPEARMRCLEDVRAQMVRRVEAQAPDAARPE"
     gene            complement(18872..19240)
                     /locus_tag="VKZ48_01125"
     CDS             complement(18872..19240)
                     /locus_tag="VKZ48_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HLU76485.1"
                     /translation="MATPAPAAHTPAALQACIDLCLDCYRSCLAEATGHCLEKGGRHA
                     DADHISLMLGCAQLCRTTAEFMLMQTKLHYKVCGACAEICEACAVSCEQLEDMEHCVE
                     ICRRCADECRRIALGMTKAS"
     gene            complement(19308..19622)
                     /locus_tag="VKZ48_01130"
     CDS             complement(19308..19622)
                     /locus_tag="VKZ48_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HLU76486.1"
                     /translation="MAWTLLILAGLLEIVWAFAMKESVGFTRLGPSIITLVAMIGSVA
                     LLAVSMKTLPLGTAYTVWTGIGAVGAFVVGLVVLGEPASVLRIVAAVLIVSGLVLMKL
                     AS"
     gene            19805..20353
                     /locus_tag="VKZ48_01135"
     CDS             19805..20353
                     /locus_tag="VKZ48_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76487.1"
                     /translation="MEGLYTELVGQAVWAIGVVVVSKLYVGFIKDRAKFSGQWLRAEI
                     RWTPEWAAEQLIGRPAQDAHSEGKIALSWDAGRDRRTYWGLSSWSLCSGTERLSQLCV
                     EFHSFDIVRKWRFLLPRHELRSCRLRSEFRCEQNGFEYPSKFASYRVRFDSSSAEELT
                     GKVVVEGNDSGRVVGAFRAVRV"
     gene            complement(20358..20855)
                     /locus_tag="VKZ48_01140"
     CDS             complement(20358..20855)
                     /locus_tag="VKZ48_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HLU76488.1"
                     /translation="MDIDTAMQDKDDANLATELLRHDHAVIRQLFDQFQDAIDEDGHA
                     RQAIAQEICMQLELHSRVELEVFYPAVHEEDPGFVDNAVDDHEDIAATIEEIRELPAA
                     SEEYEDYILELMDMVEEHVTEEEEVLFPDLEARIPDTLATLTDDIIAFKERVVGSVED
                     LEGRA"
     gene            complement(20943..21437)
                     /locus_tag="VKZ48_01145"
     CDS             complement(20943..21437)
                     /locus_tag="VKZ48_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76489.1"
                     /translation="MTPASFAIVEILIWGLIATLAMGAILFASQNFGWSRLDLPFLLG
                     TVFTGERHAANVLGFTLTILVGWFIAFFYYLFFAAFGEANWWLGAAAGFVHGLLVLTA
                     LLPLMPYIHPRVASEYDGPSLRRRLEPPGFLGLHYGYRTPVVTLIAQTVYGAVLGAGF
                     ELVF"
     gene            complement(21434..23308)
                     /locus_tag="VKZ48_01150"
     CDS             complement(21434..23308)
                     /locus_tag="VKZ48_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HLU76490.1"
                     /translation="MPRDSAEPNTSPAFKWDYPPIGDYAVIGDCRTIALVSREGSVDW
                     LCLPHFSAPSWFAALLDKDGGGRFAVRVRELRQAAHRYVADTNVLVTQMHGPGGTMQL
                     TDSVSVISEDRALELAPEHELIRLVECTEGEVDVEVFFQPRPHYGLSVPKLRDRGKLG
                     WQCVCNGDVAFLQTDIPLREIEPGVLYGTTRLRAGDKRQVVYGYCENDVSIIAPLGPA
                     IEHRVEATQRWWKQWSAQCLYEGPYRAAVLRSALTLKLLTYSLSGAVIAAATTSLPEG
                     DTGDRNWDYRYCWLRDTSLVLQSFIDLGFYAESESFLAWMLHATRLTQPRLQVMYDIY
                     GETALTERELPHLSGYRNLGPVRIGNGAHDQLQLDIYGDSVQTAYDFVDRGGYLDRYE
                     RRLLRGFGRVVLNCWRLPDAGIWEIRGEPRHNTYSKLMCWTTLHRLLELDKRVGLKLD
                     RDLYERELQSIREDIDRHAYDPARNTYVGYYGGTVPDASLLLMARYGFLEARDPRMVG
                     TYRFIERELGVDGLLFRYPPGAGYDGVGGSENLFGICCFWLVDYLARLGDRDKAQRLF
                     EQLLGYASDVGLYAEEFDLHTKAPLGNFPQAFTHVGLITAALSLEQAHEGRRGQEISK
                     "
     gene            complement(23439..24461)
                     /locus_tag="VKZ48_01155"
     CDS             complement(23439..24461)
                     /locus_tag="VKZ48_01155"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HLU76491.1"
                     /translation="MTQLKLSRVAAALTPVLAGMTLAFNVQAANETQSTVTAPGAQHG
                     QSAEAQQQMQDVRASELIGTAVEGSDGKRLGDIEDLVIDLNSQRVHYAVLGHGGIAGI
                     GEKLFAYPVSALQPGQEGRLRLNVSAEKLKDAPGFDRQHWPKWGKDDYQARVDRYYGE
                     ATPVKPDEDPRYVRASDFIGKDIKDRSNQDVGEIEDVIVNMKDGSLHYLLVEFDDWHE
                     NNAGRLFAVAPNGLRADPEDKDDLKLDVDRQTLASAPGIEIKRLRDKRDQSWIAQMKR
                     DLDSSPPMRGAVGATPSSDAATATSPGGRAQTERLQQGPEPMHPGGGQRATGTPADLP
                     QPVDTQ"
     gene            complement(24574..24939)
                     /locus_tag="VKZ48_01160"
     CDS             complement(24574..24939)
                     /locus_tag="VKZ48_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76492.1"
                     /translation="MNTKLPFATTSLRVGAGFLIWAAHFAAVYVYVALVCARGDPAAQ
                     WLGTSTTRLNIGLITLIAVALLGYVSWQSLRGTWHDDMQRAAQPFLAWLGAAIALFGL
                     LAVLWTAMPAIWVALCRPV"
     gene            complement(24936..27461)
                     /gene="ctaD"
                     /locus_tag="VKZ48_01165"
     CDS             complement(24936..27461)
                     /gene="ctaD"
                     /locus_tag="VKZ48_01165"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HLU76493.1"
                     /translation="MSGAMMSERNLPNPAPRPPSEFDKLCRIWRPPRGWRFLSAVNNT
                     YIGMFYVGTALLFFILAGILALLMRVQLAVPENQFLSPDTYNQFFTMHGTVMMFLFAV
                     PVVEAVGVLLLPNMLGARDLPFPRLSAYAFWAYFVGGIVFFCSLFFDVAPDGGWFLYP
                     PLSSIVFSPGVNNDWWLLGIGFIEISAIAGAIELIVGILRTRAPGMKLSEMPIYAWAM
                     LVLGGMIVFAFPAVIVCTLLLELERAFHWPFFMADKGGDPLLWQHLFWFFGHPEVYII
                     FLPAAGMVSMIVPAMAQVKLIGYRLTVLALVGTGFLSFGLWVHHMFTTGIPQLSLSFF
                     TAASMAVAIPSGIQVFAWIATFAAGRAKMHTPTLFLLGFLFIFVLGGLTGVMVAVVPF
                     DWQAHDSYFVVAHFHYVLIGGMVFPLFAAMYYWIPTVSRRALSERLGRWVFALMFIGF
                     NVTFFPMHIIGMAGMPRRVYTYAEQLGWGPLNMLSTVGAFILAAGILLFLIDMARNFR
                     PSATETAGNVWNAGTLEWAPNSVYALRSIPTVTSREPLWDQPTLAEELDAGHWYLPDA
                     PTGKRESIVTHPIDARPQYIVQLPGPAWSCFIAALGTAAFFLLLTVKLVAPAVIGGVI
                     AIAAIWYWAWTTDPGPIRLPADIGGGITLPVYATGPMSHSWWAMVVLLLVMGSMFMSV
                     VFTYLFLWTVSPDVWPAHESLPALGTPITAAAALLISAACAQYAHTRLDRPHATRVAL
                     ALGALTLLGALFLALSAQWRVGLSPTESAYGAAVYGMLSLTGVFIVSTVMMQLFTLAR
                     SCYGLLNAERRVTFDNTRLMWLYTVAQGFLTLALLHGFPRMTG"
     gene            complement(27458..28513)
                     /locus_tag="VKZ48_01170"
     CDS             complement(27458..28513)
                     /locus_tag="VKZ48_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76494.1"
                     /translation="MIDFQAQPLPLNFAVMAAAGFVVWMAGTRLSRYASVISDRTGIG
                     QAVMGMLLLAAVSSLPEIAIVITAALDDVAPLAVNNLLGGAALQITLLALADVTLDRS
                     ALTHVVAKPSVLLQGALAILMLALVVAAVLIGDHSVLGIGLWSWGILAIYLSATWTIA
                     RARDAWTAETPDAAPTADLDTGEGAPGAPREDETMSLATVLWRTALAAAVILLAGYLL
                     ARTGDAIAEQTPLGASFVGAVLLAFCTSLPEISTVYAAVKLRRYVMAVSEIFGTAMFN
                     IALIFLVDAVYGGGPVLSEAGKFSGFAALLVIALYAIYMVGLIERRNRTFLRMGIDSL
                     TALLFYFGGLAVLYQLR"
     gene            complement(28518..29498)
                     /gene="coxB"
                     /locus_tag="VKZ48_01175"
     CDS             complement(28518..29498)
                     /gene="coxB"
                     /locus_tag="VKZ48_01175"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HLU76495.1"
                     /translation="MRRARTLILLLLVPAAGCRGMQHALDPVSGEAAFISNLSWLLYC
                     GATLIFVTVMALAAYAIFGPRGRNPLAHRNLIIGGGIVFPAVTLTALMIYAFVGAPNL
                     LLRGEPELHIEVTGEQFWWRVRYLDAAGKAQFETANEIVIPAGTTVAFHLRTADVIHS
                     FWVPNLHGKLDMIPGHPKTMHVHAERTGQYRGQCAEYCGTQHAKMAFVVEVVAADAFE
                     QWRRAQGNEARAPDTPLLQRGREVFLTSGCATCHTVRGTSTGGALGPDLTHVGSRRTI
                     GAGLLPNGIGALGGWIADSQALKPGNRMPAFNSLPAADLQAVAAYMASLE"
     gene            complement(29495..30358)
                     /locus_tag="VKZ48_01180"
     CDS             complement(29495..30358)
                     /locus_tag="VKZ48_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLU76496.1"
                     /translation="MSLRGAIKLLLALGILGALAGAAMVYFGVYNVAATQQHTAPVYW
                     LLNASLKRAAAVRAKDIEPPPLDDPQRIANGLVLYREHCMNCHGAPGVPPNAFALGMT
                     PLPANLAHTAKTWPAREMYWVVRYGIKMTGMPAWEFRLPDEQIWDVIAFLVAMPKMTP
                     LDYQARAKAIGEGPLKPAQTQQREDAITAKPDPFEGNAERGAIAMQQYACVTCHEIPD
                     VVGAIAPVGPPLKGMATRGFIGGVLPNTRANMVRWLRDPQAVDPRTAMPNLGVTERDA
                     HDLAAFLETLK"
     gene            complement(30363..30746)
                     /locus_tag="VKZ48_01185"
     CDS             complement(30363..30746)
                     /locus_tag="VKZ48_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLU76497.1"
                     /translation="MSALFPLSPLRLAGALLCCLSLAACGDSSESHTAAGDPERGREL
                     LWQFGCGSCHRIEGVVGAVGEVGPPLDGIAHRVYIAGVLANTPENLVRWIRVPQEVDP
                     LTAMPNLGVTEEQARDIVAFLHRRG"
     gene            30867..31754
                     /locus_tag="VKZ48_01190"
     CDS             30867..31754
                     /locus_tag="VKZ48_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008345747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HLU76498.1"
                     /translation="MLAPWFAAGAWAHDVSRIAASTVPPDPHLSFDPWTGVPLVVSAL
                     LYGWGVIRLWRRAGRGRGISYVQLLYFVAGWLALAVALLSPIETLGTYSFAAHMLQHE
                     LLMAVGAPLMVLSRPLAAWTWALSAEGRRSAGRGMRHPLFAAAWGFITAPVPAWGLHA
                     VALWAWHAPALFNATLVDDTAHTLQHICFLATALLFWWTVLGRDARVAPGVPILSLLT
                     TLIHTGILGALITFAPSVWYSVYLTRSPLLGLDPLVDQQLGGLIMWVPAGAVYLIAAL
                     ALMLRWLRPALTGRTATLR"
     gene            complement(31759..32481)
                     /locus_tag="VKZ48_01195"
     CDS             complement(31759..32481)
                     /locus_tag="VKZ48_01195"
                     /inference="COORDINATES: protein motif:HMM:NF015317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqhA family protein"
                     /protein_id="HLU76499.1"
                     /translation="MHEPPAGQARRLRGGRIGHATALDVKPNDNGHDSFLAKTIGRSR
                     LIVIVAVIAVLLAAFSLFLLGAIIAFKTVIEAWYGVFAETIGPTDLTVRFLEIVSVML
                     KAVVFYLIGVGFYSLFISPLNLTIAMGIETLNDLETKIISVVIVIMAVHFLQRFIQHE
                     ETLDLLMMAGALALATLALVYFKTHAYREAKETKAERTQAQQHARKEMFRESHEEHDV
                     EHGGETKVSLRDAGADEEQRPD"
     gene            32627..34081
                     /locus_tag="VKZ48_01200"
     CDS             32627..34081
                     /locus_tag="VKZ48_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76500.1"
                     /translation="MDSIWMIWPGNPGLSAATWLLGAVAFLYAARTSVHELVRSTGQA
                     IAGPLRAISRWLSAAADELHARNRAVLLGQARREVEARIEREFERVSGLVTRDLDGYP
                     SLQRKLQDEIACMEEDYKKCGEVPPASPDWVEAVAAVARVKSGNEMVQKILTDISTSI
                     NVMHDKSLAEYRRAYKSRHRILGRFLPFWRSVERTLSRVDQKLEGLRSSAAIVDAQMD
                     KYGEITANSDRVAHALTVSAFTQLVVSLLVLAVAAGGAFVNFKLIALPMSEMVGAGDI
                     LAGNLRTSEVAALVIILVETSMGLFVMETLRITNLFPPIAQLDDRMRRRLLWVSGTLL
                     IVLAAVEASLALMRDMLIADKQALIQSLALGAEQVMIADKWVGNIPTIGQMVLGFILP
                     FALAFVAIPLESLIHSARTVGGVIALALMRLLAFVLRLVGHIAKHTSRVLITLYDLII
                     VVPLMIERLGKRAVAEVSPPVAPALEAEEGRRAS"
     gene            34078..34746
                     /locus_tag="VKZ48_01205"
     CDS             34078..34746
                     /locus_tag="VKZ48_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HLU76501.1"
                     /translation="MRIVFSHLAPLLLALVVALLAGCGDARNHAHAVYVLIDTSGTYA
                     QEAVKAHRIVNYLLGTLNPGDSLAVARVESRSFSEKDIIAKVTFDQRPSQANAQKRAF
                     QAAVDAFLKGVQGSRYTDITGGLLQGAEYLNESGAGTKTLIVFSDMQEELDQNTVRDF
                     PIDMTGIRVIAVNVVKLRTDNADPRRYFGRLEAWQQRLEDAGAKEWRVINDLERLDPV
                     LARR"
     gene            34845..35822
                     /locus_tag="VKZ48_01210"
     CDS             34845..35822
                     /locus_tag="VKZ48_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLU76502.1"
                     /translation="MSVAVVTGASGGIGRATAVAFGRRGWRVALLARGAEGLAGAQRE
                     VEQAGGEALIIPTDVADQSAVEAAAAQVEREWGAIDVWVNNAMATIFAPVMAVHPEDF
                     KRATEVTYLGSVWGTLAALKHMMPRDRGVIVQVGSALAYRSIPLQAAYCGAKSAVRGF
                     IDSVRCELIHERSNVRLTHVHLAAFNTPQFDWGRTTLRRKPMPVGKIFQPEVAAESIY
                     RAAQHPRREVWVGWPAAQAILGQKVLPGYLDRRLAFEAWEGQLDNEPIPPGRVDNLYA
                     PVREDRGAHGRFDARALPHSMQAWLNRNRVAVALGIALPLLALGAMRRR"
     gene            complement(35962..36696)
                     /locus_tag="VKZ48_01215"
     CDS             complement(35962..36696)
                     /locus_tag="VKZ48_01215"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HLU76503.1"
                     /translation="MRLSKRMSELGLTSRREADEWIERGWVRVDGEVVSELGSRVLPH
                     QKITIDKRATREQAARVTILLNKPVGYVSGQAEQGYKPAITLITPENHWSEDPTKQSF
                     KREHLKGLAPAGRLDIDSVGLLVLTQDGRTAKQLIGERSDIEKEYLVRVEYTKGGKLP
                     AKSLALLNHGLELDGVPLQPAKVSWQNEDQLRFVLREGKKRQIRRMCEAVGLKVVGLK
                     RVRIGRIALGKLPPGKWRYLHWSEKF"
     gene            37264..37626
                     /locus_tag="VKZ48_01220"
     CDS             37264..37626
                     /locus_tag="VKZ48_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76504.1"
                     /translation="MGQGDNGGFPALFDNDDLDRLRAEQLEREYPRSEPYRPLPIVAA
                     HYPRIAEAIRQLWGTPEGDAFFNRMLIDERGNRAGFPPDVVRALLALSELHQQAFRFR
                     KPEDIWIDDPAVGRFNGR"
     gene            37693..38769
                     /locus_tag="VKZ48_01225"
     CDS             37693..38769
                     /locus_tag="VKZ48_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HLU76505.1"
                     /translation="MSKLFSPFRLRDTLFRNRAFVSPMCQYSAEDGLPNEWHYVHLGS
                     RAVGGAGLVMVEATAVSPAGRISPGDTGIWNDAQRDRFQRISSFIEAQGAVSAIQLAH
                     AGRKASTAAPWDGGAPLEREQGGWDCLAPSALPFDKNWPTPIEMTKADIDATVERFRE
                     AAVRSLDAGFQVVEVHMAHGYLLHEFLSPLANRRGDEYGGSLENRMRFPLAVTRAVRE
                     VWPEHRPVFVRISATDWLDGGWDLEQSLVLARELRNLGVDLIDCSTGGVAPGAKIPVG
                     PGYQVRFAETIRAEVGIATGAVGLITKPRQAEQIVATEQADVVFLARELLRDPYWPLR
                     AAHALKADVHWPKQYERARPADLE"
     gene            38780..40117
                     /locus_tag="VKZ48_01230"
     CDS             38780..40117
                     /locus_tag="VKZ48_01230"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF006696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HLU76506.1"
                     /translation="MTADRHALFTDLYELTMLQAYLEEGMEKEAVFSLFVRRLPQRRN
                     YLLACGLETVLDYLENLRFDDGDIAYLRSLGKFSDRFLAWLADFRFTGEVHAVPEGTV
                     VYANEPILEVIAPLPQAQLVETFIMNQVHYQTMAASKASRVITAARGRRVVDFGARRF
                     HGTDAAIKAARAFYIAGVAATSNVEAGRRYGIPVAGTMAHSYIQAHEGEVAAFRAFAR
                     LYPDTVLLVDTYDTLAAVRKVIDLARESGDAFEVTGLRIDSGDLVALSREARRLLDEA
                     GLQHVELVASGNLDEDRVAALLEADAPFDTFGVGTAMGVSNDAPSLDIAYKLTEYGKR
                     GRVKLSADKPVFPGRHQIYRMESDGYDVHDVIGRAQETLAGRPLLGPVMKGGKRLSDA
                     TPPLDRVREHAREQVMRLPEHVRRLSPAEPPYPVQLSDALTRYQHRVSRNFLP"
     gene            40209..40772
                     /locus_tag="VKZ48_01235"
     CDS             40209..40772
                     /locus_tag="VKZ48_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HLU76507.1"
                     /translation="MSHPTEMLQPGDALLIVDVQNDFCPGGALPIERGDEVVPVLNRW
                     IDAARAAGVPVYASRDWHPAGHLSFEEREGPWPPHCVQDTAGAAFHPDLRLPPDTIKI
                     TKGARFDQDQYSAFDQTGLADQLRRDGIKRVWIGGLALDVCVRATVLDALKEGFAAHL
                     IPDASLPVTREGGEKALREMREAGAEI"
     gene            40802..41326
                     /locus_tag="VKZ48_01240"
     CDS             40802..41326
                     /locus_tag="VKZ48_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76508.1"
                     /translation="MKRLALMFVLQLFSQLAVAGNIADVFSEGIFGVKWGSAIEEVEK
                     AFPGGVTETQVGITTYAIQDGRTLFGVQREDSNSIRFSFDSNGEMNGVSIEFPLNAID
                     DYGVLFTKLNTVFGRGEDAPNSLGVHVVKWPEDQGITISLMHLPGVLGIGDLLFGIEH
                     RPEPSGVSKGDLGF"
     gene            41358..41984
                     /locus_tag="VKZ48_01245"
     CDS             41358..41984
                     /locus_tag="VKZ48_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008647030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HLU76509.1"
                     /translation="MIPADVLLLFVAASAALAIAPGPDNIYVLTQSAAYGKGTGILIT
                     LGLCTGLLFHIAAVALGMAAILKTSAVAFTVIKILGAAYLLYLAWQSFRAGSATIAAS
                     GERPLGARALYLRGILMNVSNPKVAIFFLAFLPQFADPARGAVSLQIVTLGLAFMVTA
                     FVIFCGIAWAAGRIGEWLKGSKRVQIGIHRLTGVVFVLLACRLALSRH"
     gene            42033..42659
                     /locus_tag="VKZ48_01250"
     CDS             42033..42659
                     /locus_tag="VKZ48_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76510.1"
                     /translation="MFKNAVRALAVVLVCGTASAAYAQSPGQPIPPAHFFDGPLLHVY
                     SPDSEGWLVTGRSGIGIAFGKRGTAPNETFGAQAVMFELGPEVRGDALVDFVRKRIAT
                     MNPPPRFEETTSAYRYHEARGYPCVDVNVGFHDNAAVTPSGEQRLLLRVIALYCRHPV
                     VSELGFFAAYSHRGPSGDAEIKEAAMRYIAGVTVNPRIAEQVKREPAR"
     gene            complement(42865..44064)
                     /locus_tag="VKZ48_01255"
     CDS             complement(42865..44064)
                     /locus_tag="VKZ48_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HLU76511.1"
                     /translation="MHANPELSMQEKRTAGIAADWLRQQGYEVTEGVGGTGVVGVLRN
                     GEGATVLLRADMDALPMQENTGLPYASKKTGIDPSSSRETGIAHSCGHDMHVAWLMGA
                     AQVLAANKDKWRGTVMVVFQPGEETGMGARAMLDDGLAKRFPKPDIALAQHVLPLPAG
                     NIAIRNGLVLSQSDSLRVTLYGRGGHGSSPQTTVDPVVMAAATIMRLQTVVSRELGLT
                     DNAVLSVGSVQAGSSANIIPDEAVLQLNIRTFDDEVREQVLAAIKRVVEGEARTAGAP
                     KPPVITRLPGEFPLTVNDEEATRKVTAALQQRFGSERVGSTGPASASEDFSLLPRAWG
                     IPSVYWIVGGVDPQQYAEAKKTGNLNALPSNHSPEFAPVPNPTLRAGVEAMLAAASVW
                     LVPADSR"
     gene            complement(44071..44229)
                     /locus_tag="VKZ48_01260"
     CDS             complement(44071..44229)
                     /locus_tag="VKZ48_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76512.1"
                     /translation="MVVSKRNLIAEVSMSFTTLIAVLVLLQPASAFGQGPALPSPSSE
                     LLKKLETV"
     gene            complement(44492..45280)
                     /locus_tag="VKZ48_01265"
     CDS             complement(44492..45280)
                     /locus_tag="VKZ48_01265"
                     /inference="COORDINATES: protein motif:HMM:NF006565.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLU76513.1"
                     /translation="MKLQDTRVLLTGATGGIGRALAGQLAQRGARMVLVGRDREQLAP
                     LAARLGAETLLFDLAATDGYAGLAGSAIRRLGGIDLLVNAAGVSHFGALYENDPSTIQ
                     RLVTINLIAPMSLCAAVIPHMRSGARIVNVGSVFGTIGFPYFTAYSATKFGLRGFSEA
                     LRRELGDRGIGVTYVAPRATRTPMNSAAVHRYAQAAHMVFDDADVVATNIVKAIEADI
                     AERTFGNAEPLYARLNALWPRLFDRLLRKNERLARHALKDEQLV"
     gene            complement(45277..45957)
                     /locus_tag="VKZ48_01270"
     CDS             complement(45277..45957)
                     /locus_tag="VKZ48_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HLU76514.1"
                     /translation="MTHYEALLAATQANREQLLAIPFIRDGAAGRLSLADYTAFLAEA
                     YHHVKHTLPLLMACGARLPERLEWLRQAMSEYVAEEVGHQEWILNDLRACGADAESVR
                     HGMPSQATELMVAYAYDLIARRNPAAFLGMVLVLEGTSVTVATQAAEALRDSLALPAK
                     AFSYLTSHGSLDQGHIKYYASLVDRLDDSADRSAIAHSARMFYKLYGDIFRHLDARRA
                     GTVVKEAA"
     gene            complement(45954..>46806)
                     /locus_tag="VKZ48_01275"
     CDS             complement(45954..>46806)
                     /locus_tag="VKZ48_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLU76515.1"
                     /translation="SSDIGLSGMAGFDGHQLMAQLREHGASTVILVPQMLAGLVDAVE
                     DAGARLPQLRFVAVGGAPVSATLIGRARRCGLPVYEGYGLSECASVVALNTPRACKDG
                     SVGRALPHARIEVRNDELYVTGATALGYVGAGTRPAGPWPTGDLGYLDDDGYVHLTGR
                     RRNMFVTAFGRNVAPEWVESELTAQPAIAQAAVFGESRPWLSAVIVSASREAVDDAVA
                     AANRMLPDYAQVRRWITVDTPFTFASGEITANGRLRRDVLLQRHGRQIEALYADARHT
                     ETYKENA"
BASE COUNT         8038 a        14894 c        15671 g         8203 t
ORIGIN      
        1 tggattcgta gaccgtcgcg ctggcaccgt cgagcgagat gtgcagcgta tcgagtccgc
       61 tgtccacgca ttcgcgcgcc cgtgcttcgg tcagcagcgt gaggttgctg ttggtggaca
      121 ccttcagtcc gcgcgatacc gcctcgcgaa ccatgcggaa gaaatccgga tgcatgagcg
      181 gttcgcccag tccctgcaga tgcagttcgc cgacgtcccc gaactggtcc agcaggcgcc
      241 ggaattcggc aaaaggcaga aaggcgccgc ggcggttctg cggcacgtct tcgcggaatt
      301 ggatcgcgca catcgcgcag cgcaggttgc attggcctac cggttcgatt tgcacgaatc
      361 gcggcagtgg gacctcgacg gcggggagga cggacagttc ggtgcgcgca ttcatgcgaa
      421 agccaccttg aaacgcagtg cgccggcgat ccgccagtag agggccagcg gcggaatgac
      481 ggccgaggtg tagagcatct ccgcgatgtg acgcggcgac agcgaagcgc cgcgcagccg
      541 ataggccgtg aagcccaagg tccatagcag ccatgcgccg aggccgcctg cggcgacgaa
      601 cggcatgccg ccgatcgcac cgatgatgcc gacagcgaac gcaccgaccg cgaagtagta
      661 gcgcagcggc gggccggacg aaatgcgtgt gcgataaagc tgcggatgct tcttgaacag
      721 caacgcgtcg aaataaacgt tctgctggat tcttgcgctc acgccccagg gcgcttcgcg
      781 cacgggatga atcaccaacg ccagcggtgc ctgttcgatg cgcagcccgc gttcgagaat
      841 agtgaagtgc agatccgaat cctcgcgcca cggccggcga tatcgttcgt cgaagccgcc
      901 cgcgtcctcg agcacttcgc ggcgtacgaa acaattggcg gtgatgaact cgccgcgctc
      961 cagtcccgcg gtattgcgtt cccagtcggt gggcgcgggc ggcagcggga catgcgtgcg
     1021 ccctgtcact gcggccacgc cgggttgcac cgcgcgcagg ccctcgcgta accattcgcg
     1081 cgacggaatg gtatcgtcgt cggtgaaggc gatcaccggc gcgtgcgccg catgccagcc
     1141 tgcgttgcgc gccgtggccg gaccgctgcg cggcgcctgc gagcgcacat agcgcacgcg
     1201 cgggccaccg ggattcgccg cgtcgatgcg cgcgaccagc gcgcgcgttt cgtccgaatg
     1261 accgtcgtcg gtcaccacga tctcataggc gtcgcccgat agcgtttgac gcaccagcgc
     1321 ctgcaggcaa cgctgcagca gcgtcggacg accgcatgtg gggaccacga ccgaaacgag
     1381 cggcgcgggg gtcatacgat cgcctccacc atggacgccg cgagcaacgg atcgccttgc
     1441 gcggtgatct gcttggtgag caggaaggag ccgatcacca gcgcgtcgag cggcgtgctg
     1501 taaaaggctt cgatggcatc gcgcggcgag cagacgatgg gctgtccgcg gctgttgaag
     1561 gaggtgttga ccagcaccgg tacaccggtc cgttgttcga aggcttccag cagcgcgcga
     1621 tagcgcggat tcgattggcc gtcgaccgtt tgcacccgcg ccgtgcgatc cacgtgtacc
     1681 gcggccggaa tgcgttgcgc aatatcttcg cgtacgccgt acacgaacag catgaacggc
     1741 gccttacccg tcacatccgg ttcgcgcacg aaccagtcgt ccagacgttc ttccagcacc
     1801 acgggtgcga ccggacggaa atcctcgcga tccttcagtt cgttcaggcg cgccttcatc
     1861 tccggctcga tgggcgaggc gaggatggag cgtgcgccga gcgcgcgcgg accgaattcc
     1921 atgcgcccct ggaaccagcc gatgatgtag ttctgcgcga gcagcttggc cgtttcctcg
     1981 gcgacgtcgt caagacggcg atagggaatc ttgctccatt cgagcaactg ctcgatttcc
     2041 tcgtcgtcgt aagccgggcc gtagtaggcg tggtccatgt gccagttgcg tgtgccgcgc
     2101 tgcacgtagt cgatccacag ggcggcgccc agtgccgtgc cggcatcgcc cgcggcgggt
     2161 tgcacccaga tgtcctcata aatgccggcg tcgcggatgc gtgcgttcat cacgcagttc
     2221 agtgcgacgc ccccggccat gacgagctgt cgttcgccgc ttgctttgcg cagccagcgg
     2281 gcgagagaaa gcgcgctttc ctccagcacc ttctgcaacg aggcggcgat gtcgaagtga
     2341 tgcgcttcga aggacgcgtt gcattcgcgc gccgggccga agcgcttttc cagatcgggc
     2401 ccggtgatgc gatagccgcc tgcgccgtcg agttgcacga tgtcgcggaa ggcatcgagg
     2461 tggcgcgggg tgccgtagga ggcgagcgcc atcaccttgt attcgtcgga ggaatggaga
     2521 aagcccagat agcgggtggt gtcctcgtac agcagaccca gcgagtgcgg gacttcgact
     2581 tccttgagtc gttgcagcgc accgtcgcgc cacacgccat gtgtagtcgt cgcgcgttcg
     2641 ccgcggccgt cgagcgtcag cacggcgcag gattcgaagg gtgcggcgag aaaggcgctc
     2701 gcttcatgcg ccagatggtg ttcgacgaaa tgccattggt acttgtgcgg tttgccggtg
     2761 tcgaagcgct tgcgcaagtg atgcggcgcg ccgccggcga gctggcgcgg tgcgttcaat
     2821 gcgtagagat agaacagatc gtcccacggt cccttgtcgt ttcggggtgc cgcagacggt
     2881 tcgtgcgcga atacgatggg cgcatcgtcc gccggccgtc cgggcagcaa tttgggatcg
     2941 agcgaatagg cgacatgatc gacgtcggcg agcgtgatcc ccgcttcgcg caggcagtag
     3001 tcgatcgcgc cgtagggcag ttcccacacc gtgaagggca cgggccgctt cgcatgcttg
     3061 atgcggctga agcgttcttc ttccgcagcg gcaatgacga caccgtcgct caccaggcag
     3121 gcggcgctgt cgtgaaaggc ggcgttgatt cctaacgtat acacaaggcg tcctcggtgg
     3181 cgcgttcggt gtgcgcgtgg agtgtggggg gcgacgcgtg cggttcgcgc tcctgcagaa
     3241 gttccagtgc ggcgcgcacg acggcttcgg gcggcacacc gcgcagacag aggtgatgtc
     3301 tttcggggca gacgctgcga tagcagttac gacagggcac gtcgtgcgat agcacgcgat
     3361 gcggcacttg ccagggcgtg tgctgtggat tggtcagggc gtacagatcg acgatcgggg
     3421 tgccgagtgc ggcggcgata tgtgcgggac cggtgttgtt ggcaatgagc agcgccgcac
     3481 gcgaaatgac gcagccgagt tcgcccagtc gcaaggcgcc ggccagcgga acggcgcccg
     3541 gcacctcggc ggcgagggcg tgcaccagat cgcgctcctg tgcgccaccc gttaccaata
     3601 tcgggcgatc gtccgcctgc gcaagcagac gcaaggcggc ggcatagtgc tcgaggggat
     3661 agcgtcgcga cgcggcgctg gcgcccggat gcacgacaat ccaatcgcgc gtgcttcgca
     3721 cgccggcctt tcgcagtgcg cgcaatgcgc gtgcgtcgtc tttctcgtac agcttgaaag
     3781 agagccgttc atcgttcgtg cgcgcgccga tgctcgcgac cagatcgagc tggcgccgca
     3841 cttcatgtcg caacagggct gcgggttcgc gttcgggttc ccagtcgctc aacaggtggt
     3901 agggattctc ccgcgtgtgc gcaagccgta gcggaatgtc ggccaaccag cagagcatcg
     3961 cggccggcaa ggcgctctgg ctgtaaacgg tgaagatgac cgcggcgtcg tagcgttccg
     4021 aacgcaagcg ttcgacgaag gcgtcggtcg ccgcaggcgt cggcgcctgc ggatgcttga
     4081 tccagggcgc ttcccacacc agcacccgat cgatctccgg cacgtgcggc gcgacggccg
     4141 caccggcagg cgaggtgagc aaaaagattt cgcgcgccgg cgcactgtgc ttcagcgcgc
     4201 gcagagcggg cgtggtcatg agtacgtcgc cgagcgcatc gaggcgcacg cacaggatgc
     4261 ggcgcgcgcg ccgccacaac gccgttgccg catggagggg gacggcgtcg gcgtgactgc
     4321 gcatcaggag ctcgcgagaa tgaaatgcgc ggcgcgttcg agatcgcgcg caagaaaggc
     4381 cggcgtgcgc aactcactct cgcgccactc ggtttccgag cccacgtcga ggagcacact
     4441 gcgacagccg gcgcgatgcc ccgcttcgac atcgtcgaga atgtcgccca gcatccacga
     4501 gcgcgcgaga tcgatatcgc gctcctgcgc cgcgcgctgc agcatgccgg gcttcggctt
     4561 gcggcagtcg catccgccgc cgggggggtg cggacagtaa tagaacgccg acagatccac
     4621 gccgtcctgg cccaagcgct cgcgcagatg cgaccagact tcgtcgaggg cgtcttcgtc
     4681 gaaatggccc agcgcaacgc cggcctggtt gctcaccaga accaactcga aacccgcacg
     4741 ctgcagacgg cgcagcgctt cacccgcttc ctcgcggacg gcgatacgcg acgggtccac
     4801 gttatagggc acgtcatgca cgagggtgcc gtccttgtcg atgaacaccg cgggcttcat
     4861 tgcagccgcc ctcatggcca ggaggtttcc cgcatcggca ggatcatgac ttcgggaatg
     4921 accgtggcgc cgccgacctt cagcacgccg gcgatcgcct cggccacatc ggccggttcc
     4981 tgcagcactt cgggatcgat atcggggaag cgatcgaaca ggaacggtgt gcgcattccg
     5041 ccggccacga cggccgtaac tttgatgcct tgcggccgca gttccgcatg cagtgcgtgc
     5101 gacaggccga gcagacccca cttgctcgcg tggtaggccg aagcgttggg ccaggcgcgc
     5161 ttggcggcgg tggaggcgat gttcacgatg tgcccgccgc cgcgttcgcg catatgcggc
     5221 gcgacatact ttgacagcag gaaggggccg cgcaggttgg tttgcaccac gcggtcgaag
     5281 tcgtccacgg ccaactcgtc gatggacagg gtgatatcga cgcccgcgtt gttgatgacg
     5341 atgtcgatgg ggccgtaacg ctcggtgact tcgcgcacca gcgtctcgac ttgctgcgca
     5401 tcgccgatgt ctgcgggaca cgcccagttg accgcatcgc gatgcttgtc gcggatgcgc
     5461 gcgcacgtct cctgcgcgag ttcgagcttg agatcgacgc agacgacctt gctgccttcc
     5521 tgtgccagac gctcgcacaa tgcggcaccc aggccgcgcg caccgccggt gacgatgacg
     5581 agcttgtcct tgagagtagt catagtgtgg ccacggtttc ctgagtcgtg gaaatcctgc
     5641 gcacgcgtgc aggctgcgcc gtcgcggcgt aaacgtcggc gacctgctcg gcgatctttc
     5701 tccaggtgta atgcgtccgc acatggcgga tgccggcgcg gccgaaggcg cgcgcgagtt
     5761 cggggttccg atgaaagcgc gccagacgct cggccagtgc gccgggatcg ttcggcggca
     5821 cgagatagcc cgtcacttca tcgagcacgg tgtgtttgat gccgccgacg ttggagccga
     5881 ttaccggcgt cccgcaggcc atcgcttcca gcggcgtgat gccgaagggt tcgtaccagg
     5941 gcgtggtgac gaacacgtcg gcagaagaat aatagtgacg cagcatttga cgcgggcgtt
     6001 ggccgacgaa gctgacgagt gcgcgtacgt cctcctgatc ggcgaccgcc atcagacggc
     6061 cgatttccgg tgtgaggacc ggatcgggcg cagacgactc gcctcccacc acgagcagtt
     6121 gtgcacggat gccgtgatcg cgcttgaggc agccgatggc gcggatgacg ttgtcgacgc
     6181 ccttgcgggg caccatgcgg cccagttgca gcacgagcaa ctcgtcatcg ctcacgccca
     6241 gcaccgtgcg cacgccgcag cccgcgttgt gcagttcttc gatgtcgacg ccgcagccga
     6301 tgacgtcgat gcgcgccggg tcggcgtcgt acagatcgat caggtcgtcg cggtcctgcg
     6361 ggcactcggc gatgatgcga tcggcgcgtt gcaccagttg ctcttcgatc gaagtgcgca
     6421 ccgccggaaa gcggtccgcg tccttctgat gaagccggcg cactttgccg agcgcatgaa
     6481 aggtcatgac gaagggcatg ccgagttctt cctgcaggcg cgccgcgacg atgcccgaca
     6541 tgaagaagtt tgcatggacg acgtcatagc ggcgcgaaga cggcgtgcaa tagttcgcca
     6601 tgcgcagcgc gaattcctgc atgtagggca gtaaatcttc tttcggtacg tagcggcgcg
     6661 gcccggcagg gatgtgcagc acgcgcacgc cgggtgcgaa cggaacgatg ccgggaaggc
     6721 tgtcgtcgtc gcaacgcgta tagacgtcga ccgtgtgacc ggcccgtgca agttctcgcg
     6781 cgatgcttgc gacgtagacg ttctgaccgc cgctgtctac gccgccgagc gttgcaaggg
     6841 gcgaagcgtg ttcactgatg agcgcgattt tcattgggga gccggaagag aaggagatcg
     6901 aggaggtgaa gactgcgtgt taagggcagc aagccgcatg ccgcgcagct tcaagcgctt
     6961 agaaggtgga agccgctccc gcgcattaag aagatatgga gcacgcacgc atttttcggg
     7021 gcgttgcctc gagacgacac acgctcgcgt gcatgcgaga ggcttacatt caagccgctt
     7081 gaagcagcgc gcgacgtcgg gcttgtaaag cgcttcaacc atttacggct gcggctatgg
     7141 gtctgaagcg cgggtggcgc gtccgcgccg ggcggttcgg tggaatgcgc tcaccgagag
     7201 gcggtgtgcg aaggcatagg ccttgcctcg cccagacatc accaccatgg agtccggcat
     7261 gagcgcgcag caacgccccc cgcagcatca ggatcagcag cccgggcgcg aagcgccgat
     7321 gcagccccag acacagtcct tcatgcgcga ctatgtcggc gcaggtcgac tgaaaggtca
     7381 ggcggtactc atcaccggcg ccgacagcgg catcggccgc gccgtggcga tcggcagtgc
     7441 caaggaaggc gccgatatcg ttatcgccta tctggaagaa catgaggacg ccgaggcgac
     7501 acgcgaagcc gtggagcgcg aaggcgtgca ttgcctgctg atccccggtg acatcgccgt
     7561 ggaggatcac tgccgttatg tggtggagcg gacagtcaag gaccttggcc gtctcgacgt
     7621 gctggtgaac aatgccgccg agcagcatcc gcaggagcga ccggagcaga tcagcgccac
     7681 gcaactgcag cgcaccttcg cgaccaacgt ctttgcgatg ttccatctgg tgaatgcggc
     7741 gctgccgcat ctgcagtcgg gcgcccgcat catcaacagc gcatccgtca ccgcatacaa
     7801 gggcaacccc aaactcatcg actacgcttc gagcaaaggc gcgatcgtcg cctttacgcg
     7861 ttcgctggcg cttgcgctgg ccggacgcgg catccgcgtc aacgccgttg cgcccggccc
     7921 ggtgtggacg ccgctcatcc cttccacgtt ccccgccgag gacgtggcga ccttcggttc
     7981 cgatgtgccg ctgggccggg cggggcagcc ggacgacatc gcgccctgct atcttttcct
     8041 cgcctcggcg gactcgacct atatgaccgg ccaggtgctc caccccaacg gcgggtcgat
     8101 catcaacggc tagacacgaa ccgtcggtcg acgggcccgc gctgcgatca tttgaggcag
     8161 gctgggcgtc gtccgcctcc gcacgagcgc gggcaatttc tgccatcctc gcccccgcgc
     8221 acccggtaaa tctggcctgg aactggcccg caatctgctt aaagtaggcg ggggtggaag
     8281 tttttcgtgt tttaagcccg cttatgaggt aaaacttacc gcctgaaggc agggcttacc
     8341 acgtcgtttt cagtccgcgg ccgcatggct gcgcaacaag gagatttacg ccatgccaca
     8401 tgtcctgatg gtcgacgacg accccgaggc gctggagtgg ctgtccgaat tcgtcaaatc
     8461 ggaaggcttc accgtcgcaa aagcgagttc cctgcgcgcc gcgcgcatca acctggcgca
     8521 gattcgaccc gatgtggtgt tcgccgatct gcagttgccg gacggcgagg gcatcgaact
     8581 gatccaggac ctggagacgc tggaaggcac cgagttcgtg ctgatcaccg gccacgcgac
     8641 cgttgaaagc gcgatcaacg cactgcgcca gggcgcgagc gactacctca ccaagccggt
     8701 cgatatcgat cggctgcgcg ccattctcaa gcgcgtgccg ggtccggcag aactgcgcga
     8761 ggagatcggt gtgttgcgcc aggagttgcg tggcctcggc cgcttcggcc acatccttgg
     8821 caattccccg gcgatgcaga agctttacga ccagatcggc cgcgtcgcgc cgacttcggc
     8881 caccgtgctg ctcatcggcg agagcggcac cggcaaggaa ctcgccgcgc agacggtgca
     8941 cgagttgagc cggcgcaaga agcggccgtt cctgccgctc aactgcgggg cggtgtcacc
     9001 caacctcatc gaaagcgagt tgttcggcca cgagaagggc agcttcaccg gcgcggatcg
     9061 tcagcacaag ggtttcttcg agcgcgcaga cggcggcacg ctgttcctcg atgaaatcac
     9121 cgaaatgccg cttgaactgc aggtcaagct gctgcgcgtg ctggagacgg gcagcctgat
     9181 gcgggtgggc atgaacacgc ccattccggt cgacgtgcgt gtcatcgcgg cgaccaaccg
     9241 caatcccgcc aaggcggtgc aggaaggcaa gctgcgcgaa gacctttatc accggctcaa
     9301 cgtctttccc atcacgctgc cgccgctgcg cgagcgcgcg accgacatcg aattcctggc
     9361 cgagcatttt ctcgacgtgc tgaaccagga ggaaggcacg aacaaggagt tttcgccgca
     9421 ggccatcgcc gcgctctacg cgcacaactg gccgggcaac gtgcgcgagc tgaagaacta
     9481 cgtccagcgc gtgtacatcc tcgccgatcg cgtgatcgac gtcgaattcg cgccgcgcat
     9541 gttcgccgag cccgagccca agccgatcct cacggtgcgc gtgggcagca cgctggagga
     9601 agtgaaccgc cgtctcatcg aggcgaccat ggccgaatgc ggcaacgtgc ggcgcaaggc
     9661 ggccgaaacg ctgggcatca gtctcaagac gctctacaac cgcctggcgc agtacaacgc
     9721 ggagaagggc gggcaggaga tgcagccggg cgacgaggaa gaggaatacg aaaagagctg
     9781 agcgcgcttc gtcgaggaac caggggccac ttgcgcggcc tctggttttt acggcgcgac
     9841 ctgcttactt cggcgcccgc ttcgattgga ggaagtgcga cttgtcgatc atggcgtgaa
     9901 tgcccttggt cttgtcgacc acttgcgaaa cctggtagtc ggtgacggcg gacatgtagg
     9961 cgagggcggt cgcgcgatcc atgttcgcaa gagcggtgcc cccaggcacg cagaggcatg
    10021 ggcgttgcat cgtgcggcgg tgcaacaacc ggaggcaagg atgtaccgac cgttcatcac
    10081 gcgcgagacc gcgcaacagg ccgtcggcgc ggtgcgacct ctgttcgaac gcgcagtcgg
    10141 cacgccggat ttcggcgaca gcggctgcgc gcatgtcgtg gtgatggacc cgcatgcgcg
    10201 cgtcggcgtc gtcgccttcg agcaggcaat cctctacgag gagagtttca accgcgaacg
    10261 ctgggatgcg gactatgcga tgtacgcgcg cgccaaggcg cgcgtgtcgt ggcgcacggg
    10321 acgcgacagc gacggcctgt cgatcgctgc gccgcaactg ctggagacgg gcgacacgct
    10381 gctcggtggc gcgatctgtg ccgacgggat cgtggtcgcg gtgagcggaa tgtatgcgtg
    10441 gtatgacgaa gcgttggcga tctgcgttgc cgcgttcctg cgcgccgtgt tgaaagggcg
    10501 gctcgcggcc tgcgacaaag ccttcatcga ataagcggtg cggccggatg cccggccggc
    10561 atcagcgctc gggcaggaag cgtttggcga acgcttcacc cgaatcggcc ggtgtgcgcg
    10621 caagcgtttc ctcgccgtcg tgggtctctt cgtcgcccgg caacgccaca tccatttcga
    10681 tttcggacag caactcgtcc ttgcgcgcct gcatctccgc gcccagttct tcgaggtcga
    10741 gttcgttgag tttgcgcacg cgaggaaaga gctgcttttc ctcgtccttg atgtgctgct
    10801 gcacatattc cgccagcacc gcgaacgtcg ccgcgtactc gggttcaccg ggctcgagct
    10861 cgcccaaccg gtcgatcagc agttcggcca cctcgtgttc caccgccgct tcgagaagca
    10921 ggctctcgcc ttcgtcgccc agcttcgagc gtaccgccgg atagaagatt tcctcttcga
    10981 tgatggcgtg cactttcaat tcgacgcacg cggtctcgac gatctcgacg ccggacgcct
    11041 cgtcgccgcg gtcgcgcttg tcgaacgcct tgaacagttt ttgcacggcg cgatgatccg
    11101 cggtcagcat ggtgagtgca ttcggcggtt gcggccgttg gcgggtaggc attcggttcc
    11161 ccagtctgtg aacgaccctg gggcagcaac ggccataccg ggggctcgca ttagcgcgtc
    11221 ggccggcggc gacaggcacg ggcggtgcat cacacaggga aaccaggagc gccttatggc
    11281 caagaaggat cccaccaaag tgccctcgcc caagggtgtc gtcgatccgg aggcgctcgc
    11341 gcgccagcag aacgacatcg agaaagaggg cggtatgcgc cggcatgtat cggatcacga
    11401 tctgcgtcag ccgaacgaac gtgacacggc accggacggc ggcatgcgtg gcactgatcg
    11461 cgcgaccgat ctgccgcaga aggacatgcc gcgtgcgcac gaggacatcg agcgcggcgt
    11521 gcgcgatacc gatcgcggca cgccttcgaa cgtgcccagc agcgccgaca accgccgggc
    11581 gacggaaaag cagggcgcga aaggcgggtg acgggcatag ccgttgcctt tgcagcactg
    11641 cacgacatta cattcacatc gacactacat agagagggaa tcatgcagac atcggacatg
    11701 caaggcggca cgggcggcag cttcgatacc gaggcgatgc gcgaacgcgc ttccgcaaat
    11761 atcgaacgcg cttcgtcctc cgcgcaccag accgtcgaca aactcgcatc gcaggcgcag
    11821 gcggcgaagg agcgtctggc ggcttccggc gagcagtggg ccgaacaggg tcagcaatac
    11881 ctggagcaga cgcgcgacat ggtgcgcgcc aatccgctcg ccgccatcgg catagcggtc
    11941 gccgccggca ttctgttgtc gaagctgacg tcgcgccatt aaagcctcat ggccgcatcg
    12001 gcggaagaac ttacacgtca gcaccaggcg gcgctcgaag cggcttcgcc cgagtaccgg
    12061 gcccgtttcg acgccgcgcg taacgagccg ctcgaagggc gattcgatta tctgtggcgc
    12121 acgacgcgac gcgtggcgca cggctatgcg ctgctggccg cgctcgatat gcgtcgcgcc
    12181 accatccagc ttgcgtggct ggtcggctcc ggcgtaatga ttgcggtgct gggcatcacg
    12241 gcatggctca ccctggtgac ggcgctcgtc acctggctgc tgcaggagaa cgtatcgctg
    12301 ccgctggtgc tggtcatcgc cgcggtggtg aatctggtcg gcgccggctt catcgtgtgg
    12361 cgcatgcgca cgctggtgtc cgaaatgcct ttcgccgcca ctctgcgtca gttgaagggc
    12421 gaagcgccgg agaccgagcg cacgcctggc gagaccggcg catgaggtgg aaaccttcct
    12481 ccctcgacga cgaaatccac gccgtcgaac gttgcattgc gtcggatcgc aggctgctgg
    12541 agcaggcttt cgtcggctat gtggacggcg tgcgcgacag cgcgctacac accatggcct
    12601 cgcccaaatt tctgctgggt gcgctcggcg tcggcttcgt catcggcaag ttcctgttcc
    12661 ggccgcggaa ggcgagccgg cagaacgagg cgacggcgaa gcgcagtgtg ctggggctgc
    12721 tcggcgccgg caccttgtcg ttggtgcaag cgcagttcgg cgggccgctg gggctcgcgc
    12781 gctggcttgc caagacctat gcgtcgcgtc gtccgacgca gcgcatgccg gctgccccgc
    12841 cgcagcctgc accggtcgtg catgcgcaac ccgcggtcgt cgccacgccg gcgggagaag
    12901 agccggccgt gctgctgcga cgttgattac ggtttgtccg ctgctgccgc cgttcgacgc
    12961 ctgaaacaca acgcctgcgg agtacccgat gagggttctt cgtgcggccg ctgtttcagt
    13021 ccttcgatcg ttgttcttgt cccgctgcac gcgctttctc ccgttcgttc tcggcggggg
    13081 cattcttctt cggttcggtt tgcgcgcgcc ggctgaactg gccgcagtcg ctgtcctgcc
    13141 ccggcccggt ctcgatcaac ggcaggatcg ccagcagtgg attgaccaag ccgagcgcga
    13201 ccgccgccac gcccttggcg gccagcttgc cgcctttgat gccggcgccg ggatcggcca
    13261 gcgtgccgga caggtgcagc ggcgtgcgca gcgcgaggat gctttcgtcc ttcggccgcg
    13321 gatggatggt cacgtcgatc tcctcgttgg cgaaattgac gtggccttcg ccgatgaaca
    13381 ccgtgtcgtc ggtatcgaga acgaacagct cgctctgcgc gaggccgttc tcgatgtgca
    13441 ggtccaggat ggcgcagcgc agcggcaccg gcttgccctt gatggtgagg aacttcacca
    13501 cctcggcgat atcgagtccg gccgcttcca gcaccagcgc gctcatctcg ccgccgtcca
    13561 ccatcagcgt cacatcgccg tctgcgctgc cgagcagttt cgcaaccgat gcgccgtgtc
    13621 cctcgatccg cccgcgaccg tacagcgtgc cgaccgcgac atcctctttc tgcagatcgg
    13681 gcagcagctt cgccaggttg acttgccgca cgtccatgct cgccgacgcg cgcggcggct
    13741 tggcgcgtcc ttcaagcaga agcgccgttg ccaccttgcc gcccgcgact tcgaagtcga
    13801 gcggttgcac ccgaaccgtg ccgttgtcga cggtgagatg accggccagc gcattgatcg
    13861 gcaccgcatc ggggcgcctg acctgttcgg cgcgaaagcg aacgtcggca tccaggatgt
    13921 cgagcgcggt gaagctcagc ggcttgtcgg ggaagatggt gtcgtcgcgc ttctgcgttt
    13981 gcgccttggc ccgctgcgcg gcggaagcgg tttcgcccgg tccggtcttc ggcgccgcgc
    14041 cgaccagcgg cagcacgtcg tccagatcga gcagcttgac gtcgaactcg ccgctgaaga
    14101 agggccgcgt ttcgcgcaac tcgagggcga aatgtccggc aacatcgctg tcgccgtagc
    14161 ggccggccag accgtccacc tgccaatggt cgcgtgcccg gttgcgcaga cggccgcgca
    14221 aagcgtaggg cggcgtatcc ggcaattcga tttttcccag cttgttgagc gcagcgagac
    14281 tgtcgccggc gatgctgagc tgcatgtcga gaccgtcgat acccagcgag gtgagacggc
    14341 cggcgaccgc ggcggtggtc cggtcgaggg tcagcgcgag cgaaaccatc accggtgcct
    14401 cgggcgaggg cagcgcggcc gggctgtgaa gcaccgcgtg cacatccgtc tcgtgcacac
    14461 ggccttgggc gcgcacggcg acgtccggct gctccggcgt gccgccttcg gcacgcagtt
    14521 gcagttccgc accgagaccg gcatcgcgaa aatggatgag cacattgctc aggcgcacgt
    14581 tgttgatgat cggcggcggt tcgtccttct cctccttctg ctccgcgccg atggcccagg
    14641 tggcgcggcc ctggctgtcg cgctcgaggt tcagttccgc gtcgatgagc gccgcggatt
    14701 tcacgcgcgc agtgccggtc agcagcggca gccaggccag acaggcactc acatgccgcg
    14761 cgttgagcag gtgctcgcgc tcggcccagt ccggattgcc gatgcgcacc tcgtccgcct
    14821 cgatgcgcag acaacggcgc agatcgatgt ccaggcggcc gatggagacc tcgcgtccgg
    14881 tcagcttttc gatctggcgt tcggcaatgc cgcgtaccca tgcggattgc acgacgagca
    14941 gcagcgccgc gaccagcacg aacagcgtga gggcggcgcc gatgaggatg cgcttgatca
    15001 tgcctccccg ctgatgtcgc ctgcttgccc cataatcccg acgtgcgcca tccgccgcgc
    15061 aaatgagaga atcgcgaaac cggcatgcga cgtcgggctg cgcatcggac ggaaacggcg
    15121 gctgcacaaa ccggcgcgcc gtgcaggagg gagacgccgg cgacgctgca agccacatgc
    15181 cgcaaggaga gcgtattggc agggcctacg accgaacttc tcgcattgag cgccgatccg
    15241 gcggaccggc aacggcttgc cgaacggctg cagcgtgcag ccaatgctgc gctggctttc
    15301 gcggtgttcg tgatcgcgct gccggtgatc ggctggatcg ccaatgtgga cgagttgcgc
    15361 cggctgatcc cgggctggcc gcccatgcat cccctcatgg cggtgttcgg catcggcggg
    15421 gcggcgacgc tgcggctgct ggcgcgcagt tcgcccaagg ctcgccatgc cgccttcgcc
    15481 gtctgcgcct cgatgctggc gatcgcgctt gccgtggtgt ggttggaaag cgatcgcgtg
    15541 tgggcttacg aaatcagccc ctatgccgcg ctgaccgcca gcgccgtgct gttcgcgatc
    15601 ggctgcaaat tctggtcgaa ctactgggtg cgacgcacgg cggatttctt cgccggggtg
    15661 gccggtgcgc ttgccatcgc cggtctggtg ggtgtgacct atcgcttgat gatcgaaatg
    15721 ccggcgctgg tggagttgtc gcttgccggt ggcctgtccg tgctggcgct ggcctttgcc
    15781 gtcttcgccg gccgtcccga cgagtggctg ctcgatcaac tcggcgaccg ccgtgtcggc
    15841 gccatcgtgc tgcgccgttc gttgccggtg gtcgtggtgt tccccatcct gctcggcctt
    15901 gcgcgcatgg cggcgcagcg tgccggttgg ctggataccg ccgccggcgg actgattctc
    15961 accgtgctga ccgtgctggg cctggcggcc ttcgtgctct ggctggcgca tgtgctcaac
    16021 cgcgtggacg ccgagcggcg cagcgccgaa cgcgaagcgg agacccagcg cgagtggctg
    16081 cgcgtgacgc tgggcagcat ctacgatgcg gtgatcgcca ccgatgccga ttgcgtcgtg
    16141 cgcgtggtga acgacgcggc ggcacggctg ctgggccaac cgatcgacgg tctgttgcgc
    16201 gaatcgctcg ccggccgggt ggccattcac gacgagaacg gaccgatcga ctgtccgctg
    16261 agtgccgtgc tgcgcggcga ggtaggggcg cgggattatc gcgatctgca gttggaactg
    16321 cctggcgaca aacgtctgcc catcgaggtg agcgtcgcgc cgatccatga cggcgagggc
    16381 ggcatttccg gcggcgtgat ggtcattcgc gacgtcagcg aacggcgcaa gcacgtgcag
    16441 gtgttgcgcg aagcctacgc cgaactcgac cggcgcgtgg ccgaacgcac cagcgccctg
    16501 caacaggcga atgccgcgct gcacgaaacg ctggcgctgt tccgcggcgt ggccgaaagc
    16561 acgcccgacc tgatcttcgt gaagaacctg caacagtgca tggtaatggc gaaccccgcg
    16621 acctgccgcc tgttcggccg caccgagcag gaactgatcg gtcagcgcat cgccgacctc
    16681 atcgacgatg aagacatcgc gcgcggtgtc gacgaacacg acaagcgcgt gctgcattcg
    16741 gggcggatcg agcgcgtgga acaggtgctc aagacgccgg aggggtatcg caccttcctg
    16801 tcgaccaagt cgccgctgcg cgacgtcaac ggcagcatca tcggcctgat caccgttgcc
    16861 accgacatca ccgagcgcaa gcgcatcgaa aacgatttgc gcgaggcgca gcgattcacg
    16921 cagggcctgc tcgataccgc gccgctggtg ctctatctgc tcgatctgga tcacggccgc
    16981 atcgtgttcg ccagcggcat ggtgctgcag gcgctcggct acgaggccga ggcgctgcgc
    17041 gcgatgtcca cccacggtct gctggaactg gtgcatcgcg atgaccgtca ttcgctcatg
    17101 aaacatctgc gcgactatcg cgacggcgag gacggcctgc gcacggtcga actgcgcttc
    17161 cgtcatcacg acggcgactg gcgctggctc tactgccgcg agcggctgtt cgatcccgtc
    17221 gcgcgcacgc gcctggtgct gggcgtcgcc atcgacgtca ccgaccgccg gcaggccgag
    17281 cgcgaactgg aacagctcat cgacacggag aaacgcctgc gccacgaagc cgagcgcgcc
    17341 aaccgcgcga aggacgagtt tctcgccgtg gtctcgcacg aactgcgctc gccgctcaac
    17401 gcgctgcgcg gctggagttt tctgctgggc aactccaatc ccgtcgatgc aaacctcctg
    17461 gaacgcgcgg tgcaggcgat caagcgcaat gtcgatcatc aggcgcggct gatcgacgac
    17521 cttctcgata cctcgcgcat catgagcggc aagctcaacc tggagcaccg gccgctcaac
    17581 ctcatcgaaa cggtgaacgc cgccatcgaa gtggtgcgac cggccgcggc ggcgaagcgc
    17641 atcgatgtgg tgttcgatca tccgcccggt gcgctcagcg tggagggcga tccggcgcgg
    17701 ctgcaccagg tggtgatgaa tctgctgtcc aacgcggtga agttcacgcc cgaggaaggg
    17761 cacatcggca tcgtcgcgga agtgacgccc gacgacatgg cgcgcatcgc ggtgacggac
    17821 accggcgtcg gcatcgatgc gcagttcctg ccgcgtctgt tccagcgctt cacccaggcg
    17881 gactcctcca ccacgcgcaa gcacggcggg ctgggaatcg gtctggcgct ggtgcgccat
    17941 ctgatcgaat tgcacggcgg ctgcgtcacg gccgagagcg aggggttgaa caaaggggca
    18001 acgttcacgg tgctgctgcc cctcccgcag gtccgcgtgg cgacggagag cctgcccagc
    18061 ggcgcggcgg ccgaaggcag cgacgcgccg ctggccggcg tgcagatctg tgcgctggac
    18121 gacgatcccg atgcgcgcga catcattgcg cacgccctgc gccgtgccgg cgccaccgtg
    18181 cacagcgcgt cctccggcgc ggaactgatt gcgctgctgg acgagcagtt gccggacacg
    18241 cggccggatg tgctgctgct cgacctggcc atgcccgggg aggacggctt cggcgtgctc
    18301 gccaatgtgc gggcgctgga aaagcgcaag ggcgtctcgg aggacgacgc cataccggcc
    18361 atcgcggtca ccgcctttgc cgaattgagt cgcgtgcgca tcgtcgaagc cggcttcatc
    18421 gaacgcgtgg ccaagccgtt cgatccgggc ctcctggtca acaccattcg tcgcgtgctg
    18481 gccggacatc gcagcgatgc ggcgccgctg cgcaatcgca tggccggtta gtgaggatcg
    18541 ccgcggtacg gcgcttgcgt ggcgtggagc gtcgttcggc tcgaatccgg aacctcgatg
    18601 cgcctcttcc tgctgctcgc tctgtgcctc cacgccgcgc ccgttgcggc cgatgaaaag
    18661 gtgcagaatg ccgaaggcgt agtggcgccg agtgggcaag cgggcgagca ggccgagcgc
    18721 agtgcggcgc gcaaggcgga tgcggattgg atcaagcgtt gcgacaagct tgcgcccgag
    18781 gcgcgcatgc gttgcctgga ggacgtgcgc gcgcaaatgg tgcgacgggt ggaggctcag
    18841 gcgcccgatg cggcgcgacc cgaatgagct ttcaggatgc cttggtcatg ccgagcgcga
    18901 ttcgccggca ctcgtcggcg caacgtcggc agatctccac gcaatgttcc atgtcttcca
    18961 gttgttcgca gctcactgcg caggcttcgc agatttcggc gcaggcgccg cataccttgt
    19021 agtgcagttt cgtctgcatg agcatgaact ccgccgtggt gcggcaaagc tgcgcgcagc
    19081 cgagcatgag cgagatatgg tccgcgtcgg catgccgtcc gcctttttcc aggcaatgcc
    19141 cggtcgcttc cgcaaggcaa ctgcggtagc agtccagaca aagatcgatg caagcctgca
    19201 aggcggccgg cgtgtgtgcg gcaggggcgg gcgtggccat tgtcggtctc cggtggatgt
    19261 gccggtgggg aggcaacacc cgttcccggc gacgcccgcg cgagccgtca ggaagcgagc
    19321 ttcatcagta ccaggccgct gacgatcagc accgccgcga cgatgcgcaa cacgctggcc
    19381 ggttcgccga gcaccaccag gccgaccaca aatgcgccta ccgcgccgat gccggtccat
    19441 acggtatagg ccgttcccag cggcagcgtt ttcatcgaaa cggcgagcag ggcgacgctg
    19501 ccgatcatgg ccacgagggt aatgatggaa ggccccagtc gggtgaagcc tacggactcc
    19561 ttcattgcga aggcccagac gatttccaac aggccggcga gtatgaggag ggtccaagcc
    19621 atgcggctct ccttgttttg cagagccggg ccgtcccgga cgttgttctt cggaggcgag
    19681 gtcgtccacg ccgcgggcga ttctactaca gcagcatgga cggttcgccc ggcggacggt
    19741 tgacgcagca ccgctgccgg ccgcaaattc ggcggcgcgc gacgatgcgc ggggaggaga
    19801 cgctatggag gggttgtata cggaactcgt cggtcaggcc gtgtgggcga tcggcgtggt
    19861 cgtcgtcagc aaactctacg tgggtttcat caaggaccgc gcgaagttca gcggtcagtg
    19921 gctgcgcgcc gaaatccgct ggacacccga gtgggcggcg gagcaactga tcgggcggcc
    19981 ggcgcaggac gcacactccg aaggcaagat cgcgctgtcc tgggatgcgg gccgggatcg
    20041 acgcacctat tggggcttga gttcctggtc gctttgctcg ggcaccgagc ggctgtcgca
    20101 actgtgcgtg gagttccaca gcttcgacat cgtgcgcaag tggcgcttcc tgttgccgcg
    20161 ccacgaactg cgcagttgcc gcctgcgctc ggagttccgc tgcgagcaga acggcttcga
    20221 gtatccgtcg aaattcgcca gctaccgcgt gcgcttcgat agcagttccg cggaggaact
    20281 gaccggcaag gtcgtggtcg agggcaacga ttccgggcgg gtggtgggcg cgtttcgcgc
    20341 cgtgagggtg taaagcctca ggcgcggcct tccaggtcct ctaccgagcc gaccacgcgt
    20401 tccttgaagg cgatgatgtc gtccgtgagc gtggcgagcg tgtcgggaat gcgcgcttcc
    20461 agatccggaa acagcacttc ctcttcttcg gtgacgtgct cctccaccat gtccatcagt
    20521 tcgaggatgt agtcctcgta ctcttcgctt gcggccggca gctcgcgaat ctcttcgatg
    20581 gtggcggcga tgtcctcgtg atcgtccacc gcgttgtcga cgaagcccgg gtcttcctcg
    20641 tgcaccgcgg gatagaacac ttccagttcg acgcggctgt gcagttccaa ctgcatgcag
    20701 atctcctgcg cgatggcctg acgcgcatgg ccgtcctcgt cgatcgcatc ctggaactgg
    20761 tcgaacaact ggcggatgac ggcgtggtcg tgacgcagca gttcggtagc cagattcgcg
    20821 tcgtccttgt cctgcatggc ggtgtcgatg tccatgacga tcctcccgga tgagatggct
    20881 ttggcgctgc agcgagcgtt cccggaagcg gcagggttat gacgcgggcg ggctgtcaaa
    20941 gatcagaaaa ccagttcgaa acccgcgccc agcacggcac cgtagaccgt ctgcgcgatc
    21001 agggtgacaa ccggcgtgcg gtagccgtaa tgcaggccca gaaagccggg cggttcgagt
    21061 cggcgtcgca ggctggggcc gtcgtattcg gacgcgacgc gcggatggat gtagggcatg
    21121 agcggcaaca gcgcagtgag gaccagcagg ccgtgcacga atccggcggc ggcgcccagc
    21181 caccagttcg cctcgccgaa ggccgcgaag aagaggtagt agaagaaggc gatgaaccag
    21241 ccgacgagga tggttagcgt aaagccgagc acgttcgcgg catggcgctc accggtaaag
    21301 accgtcccga gcaggaaggg cagatccagg cgcgaccagc cgaaattctg actggcgaaa
    21361 aggatcgcgc ccatggcgag cgtcgcgatc aggccccaga tcaggatctc cacgatggcg
    21421 aacgaggcgg gcgtcatttg ctgatctcct gaccgcggcg tccctcatgc gcctgctcga
    21481 gcgacagggc ggcggtgatg aggcccacat gggtgaaagc ctgcgggaag ttgccaagcg
    21541 gcgccttggt atgcaagtcg aattcctccg cataaagccc gacgtcgctg gcataaccga
    21601 gcagttgttc gaacagccgc tgcgccttgt cgcggtcgcc cagccgcgca aggtagtcca
    21661 ccagccagaa gcagcagatg ccgaacaggt tctcgctgcc gcccacaccg tcgtatccgg
    21721 ccccgggcgg ataacggaac agcaggccgt ccacgcccag ttcgcgctcg atgaagcgat
    21781 aggtgccgac catgcgcgga tcgcgcgcct ccagaaagcc gtaacgcgcc atgagcagca
    21841 ggcttgcgtc gggcactgtg ccgccgtagt aaccgacgta ggtgttgcgc gccgggtcgt
    21901 aggcgtgccg atcgatgtct tcgcggatcg actgcaactc gcgttcatag agatcgcggt
    21961 ccagtttcag gccgacgcgc ttgtcgagtt cgagcaggcg gtgaagcgtg gtccagcaca
    22021 tcagcttgga ataggtgttg tgccgcggct cgccgcgaat ctcccagatg ccggcgtcgg
    22081 gcaggcgcca gcaattgagc accacgcgac cgaagccgcg cagcagtctg cgttcgtagc
    22141 gatcgagata cccgccgcga tcgacgaagt cgtaggccgt ctgcaccgag tcgccgtaga
    22201 tgtcgagttg caactggtcg tgtgcaccgt tgccgatgcg caccggtccg aggttgcggt
    22261 agccggagag gtggggcagt tcgcgttcgg tgagggcggt ttcgccgtag atgtcgtaca
    22321 tgacctgcag gcgcggttgc gtaagccgtg tggcgtgcaa catccaggcg aggaagcttt
    22381 cgctctcggc gtagaaaccc aggtcgatga acgactggag gaccagcgag gtgtcgcgca
    22441 gccagcaata gcggtaatcc cagttgcggt cgccggtatc gccttccggc aatgacgtcg
    22501 tcgcggcggc gatgaccgcg ccggacagcg aataggtcag caacttgagc gtcagcgcgc
    22561 tgcgcaacac cgccgcgcga tacgggccct cgtagagaca ttgcgccgac cactgtttcc
    22621 accagcgctg cgtcgcctcc acgcgatgtt cgatggccgg cccgagcggg gcgatgatgc
    22681 tcacatcgtt ctcgcaatag ccatacacca cctgccgctt gtcgccggcg cgtagcctgg
    22741 tcgtcccata gagtacgccg ggttcgattt cgcgcagtgg aatatcggtt tgcaggaagg
    22801 ccacgtcacc gttacaaacg cattgccaac cgagcttgcc gcggtcgcgc agtttcggta
    22861 ccgacaagcc gtaatgcgga cgcggctgga agaacacttc cacgtccact tcgccttccg
    22921 tgcattccac cagccggatg agttcatgct ccggcgccag ttccagcgca cgatcctcgc
    22981 tgatcaccga taccgaatcg gtgagttgca ttgtgccgcc cgggccgtgc atctgcgtca
    23041 cgagcacatt cgtgtctgcc acgtagcgat gcgccgcttg acgcagctcg cgcacccgca
    23101 cggcgaagcg gccgccgcca tccttgtcca gcagcgcggc gaaccatgag ggggcggaaa
    23161 agtgcggcag gcacaaccag tccaccgagc cttcccggga caccagcgcg atggtgcggc
    23221 agtcgccgat gacggcgtaa tcgccgatgg gcgggtagtc ccatttgaaa gcaggggacg
    23281 tgttgggttc ggcgctatct cgaggcactt gggcgttgcc ttgcggttcg cttcggcagg
    23341 aagcgtccga cacttcggac tccgctccgt ttcaaaaaaa caccttcaag aaaaaacggc
    23401 ggcatcgcag ccgccgtttt tttccggttg cattgtgctt attgcgtgtc gaccggttgc
    23461 gggagatccg cgggcgtgcc ggtggcgcgt tggccgccgc cggggtgcat cggctccggc
    23521 ccctgctgga gacgctcggt ctgcgcgcgg ccgcccggcg aggtcgccgt cgccgcgtcg
    23581 ctggacggcg ttgcgccgac cgcgccgcgc atcggcgggc tcgagtccag atcgcgcttc
    23641 atctgcgcga tccagctctg atcccgcttg tcccggagac gcttgatctc gataccgggc
    23701 gccgaggcga gcgtctgacg atccacatcg agcttgagat cgtccttgtc ttccggatcg
    23761 gcgcgcaagc cgttcggtgc gacggcgaac aggcggccgg cgttgttctc atgccaatcg
    23821 tcgaactcga ccagcaggta atgcaggctg ccgtccttca tgttgacgat cacatcttcg
    23881 atctcgccca catcctgatt gctccgatcc ttgatgtcct tgccgatgaa atcgctcgca
    23941 cgcacataac gcggatcctc atccggtttc accggtgtgg cttcgccgta gtaccgatcg
    24001 acgcgggctt ggtaatcgtc cttgccccac ttcggccaat gctgccggtc gaagccgggt
    24061 gcgtctttca gcttttccgc actgacgttg aggcgcagcc ggccttcctg accgggctgc
    24121 agggcgctca cggggtaagc gaacagcttt tcgccgatgc ccgcgatacc gccgtgtccc
    24181 agcaccgcgt aatgcacgcg ctggctgttg aggtcgatca ccagatcctc gatgtcgccc
    24241 agacgcttgc cgtcgctgcc ttcgaccgcg gtgccgatca gttcggaggc gcgtacatcc
    24301 tgcatctgct gttgcgcctc tgcgctctga ccgtgctgcg cgcccggtgc ggttaccgtg
    24361 gattgggttt cgttcgcggc ctgcacattg aacgccagcg tcattcctgc caacaccggc
    24421 gtcagcgcag ccgcgactcg actgagtttc agttgcgtca tgttgtctgc tcctatgccg
    24481 ttgatcaaat gcgcgtcgtt tcgacgcacc gtaccgatag gtcagcaacg cgcgtaccgg
    24541 cggcacgaca aaaccgggag atgacttgga cagtcatacg gggcggcaga gagcgaccca
    24601 gatcgcgggc atggccgtcc acagcacggc gagcaatccg aacagcgcaa tcgcggcgcc
    24661 gagccaggcg agaaaaggtt gcgcggcgcg ttgcatgtca tcgtgccagg tgccgcgcag
    24721 cgactgccat gacacatagc cgagcagcgc cactgcgatg agcgtgatca agccgatgtt
    24781 gagtcgtgtg gtcgaagtgc ccaaccactg cgcggcggga tcgccgcgcg cgcaaacgag
    24841 cgccacataa acatagaccg cggcgaaatg cgccgcccag atgaggaagc cggcgccgac
    24901 gcgcaaactc gtcgtggcga aaggcagctt cgtgttcatc ccgtcattct cggaaagcca
    24961 tgcagcaacg cgagcgtgag gaatccctgg gcaacggtgt agagccacat caaccgcgtg
    25021 ttgtcgaagg tgacgcgtcg ctcggcgttc aacagcccgt agcaggagcg cgcgagcgtg
    25081 aataactgca tcatgactgt gctgacgatg aaaacgccgg tcaaagaaag catcccgtag
    25141 accgcggccc cataagccga ctcggtgggc gaaagaccga cgcgccattg cgcactcaaa
    25201 gccagaaaga gcgcgcccaa cagcgtcaac gcgccgagcg ccagcgcaac gcgtgtcgca
    25261 tgcggccggt cgagtcgcgt gtgggcgtac tgcgcacaag ccgcgctgat caagagcgcc
    25321 gccgccgcag tgatcggcgt gccgagtgcg ggaagcgact catgcgcggg ccacacgtcc
    25381 ggagagaccg tccagaggaa gagataggtg aacaccacgg acatgaacat cgagcccatg
    25441 accagcagca gcacgaccat cgcccaccag gagtgcgaca tcggtccggt ggcgtagacc
    25501 ggcaatgtga tgccgccgcc gatgtccgcg ggaaggcgta tcggtccggg atcggtggtc
    25561 caggcccagt accagatcgc agcgatggcg atgacgccgc cgatgaccgc aggcgccacg
    25621 agcttcaccg tcagcaacag gaagaacgcg gccgttccca aggcggcgat gaagcaggac
    25681 caggccggtc cgggcagttg cacgatgtat tgcggccgcg catcgatcgg atgcgtgacg
    25741 atggattcgc gcttgccggt cggcgcatcg ggcagatacc agtgaccggc gtcgagttct
    25801 tcggcgagcg tgggctgatc ccacagcggc tcgcgactgg tcacggtcgg aatgctgcga
    25861 agggcgtaca cgctgttcgg cgcccattcc agggtgcccg cgttccagac gttgcctgcc
    25921 gtctcggtgg cggacgggcg gaagttgcgt gccatatcga tgaggaacag cagtatgccg
    25981 gcggcgagga tgaatgcgcc gacggtggac agcatgttga gcggccccca gcccagttgc
    26041 tcggcgtagg tatagacgcg tcgcggcatg cccgccatgc cgataatgtg catcgggaaa
    26101 aacgtcacgt tgaagccgat gaacatcagc gcgaacaccc agcgaccgag ccgctccgac
    26161 agcgcgcgcc ggctgacggt gggaatccag tagtacatcg ccgcgaagag cggaaacacc
    26221 atgccgccga tcagcacgta atggaaatgc gccacgacga agtagctgtc gtgcgcctgc
    26281 cagtcgaagg gcacgactgc gaccatcacg cccgtcagcc cgccgagcac gaagatgaac
    26341 agaaagccga gcaggaacaa cgtcggcgta tgcatcttcg cgcggccggc ggcgaaggtg
    26401 gcgatccagg cgaacacctg tatgccgcta ggaatggcaa ccgccatgct cgccgccgtg
    26461 aagaagctga gcgacagttg cggaatgccg gtggtgaaca tgtgatgcac ccacagtccg
    26521 aagctcagga aaccggtgcc gacgagcgcg agcaccgtga ggcgataacc gatcaacttc
    26581 acctgcgcca tggccggcac gatcatcgat accatgccgg cggcgggcag aaagatgatg
    26641 tacacctccg ggtggccgaa gaaccagaac aggtgttgcc acagcagcgg atcgccaccc
    26701 ttgtcggcca tgaagaaggg ccagtgaaac gcgcgctcca gttccagcag cagcgtgcag
    26761 acgatcaccg ccggaaaggc gaacacgatc atgccgccca gtacgagcat ggcccaggcg
    26821 tagatcggca tctccgacag cttcatgccc ggtgcgcgcg tgcgcaggat gccgacgatg
    26881 agttcgatcg cgccggcgat ggcggagatt tcgatgaagc cgatgcccag cagccaccag
    26941 tcgttgttca cgcccggcga gaagacgatg ctggacagcg gcgggtaaag aaaccagccg
    27001 ccgtcaggtg ccacatcgaa gaacaggctg cagaagaaca caatgccgcc cacgaaatag
    27061 gcccagaagg cataggcaga cagacgcgga aagggcagat cgcgtgcgcc gagcatattg
    27121 ggcagtaaca gcacgccgac cgcttcgacc accggcacgg cgaacagaaa catcattacc
    27181 gtgccgtgca tggtgaagaa ctggttgtac gtgtccggac tgagaaactg gttttccggc
    27241 acggcaagct gcacgcgcat cagcagcgcc agaatgccgg cgaggataaa gaacagcagc
    27301 gccgtgccga catagaacat gccgatatag gtgttgttga ccgcgctgag gaagcgccag
    27361 ccgcgcggcg ggcgccagat cctgcaaagc ttgtcgaatt cgctcggcgg gcgcggtgcc
    27421 gggttgggga gattgcgttc gctcatcatc gcgccgctca tcgcagttga taaagcacgg
    27481 cgagcccgcc gaagtagaag agcagcgcag tcagggaatc aatccccatg cggaggaagg
    27541 tgcgattgcg ccgctcgatc aaacccacca tgtagatcgc atacaaggcg atcaccagca
    27601 gtgccgcgaa gccggagaat ttccccgctt cggacaacac cgggccgccg ccgtaaacgg
    27661 cgtcgacaag gaagatcagc gcgatgttga acatcgccgt gccgaagatc tccgacaccg
    27721 ccatcacata acggcggagt ttcaccgccg catacaccgt gctgatttcc ggcagcgaag
    27781 tgcagaaggc gagcagcacc gcgccgacga agctcgcgcc cagcggcgtc tgctccgcga
    27841 tcgcgtcgcc ggtacgcgcg agcagatagc cggccaggag aatgacggct gcggcgagcg
    27901 ctgtacgcca tagcaccgtg gcgaggctca tcgtttcgtc ttcccgcggt gcgccgggtg
    27961 ccccttcgcc ggtgtcgaga tcggccgtcg gggccgcgtc cggcgtttcc gcggtccacg
    28021 cgtcgcgtgc gcgcgcaatg gtccacgtgg cactgaggta gatcgcgaga atgccccagg
    28081 accacaagcc gatgccgagg accgagtggt cgccgatgag gacagcggca acgaccagcg
    28141 caagcatcag tatggcgagc gcgccctgca acaacacgct cggcttcgcg accacgtggg
    28201 tcaacgcgct gcgatcaagc gtgacatccg ccagtgcgag cagcgtgatc tgcagtgccg
    28261 caccgccgag caggttgttt accgcgagcg gcgccacatc gtccaacgcg gcggtgatga
    28321 cgatggcgat ttcgggcagc gaactgacgg ccgccaacaa cagcatgccc atcaccgctt
    28381 gtccgatgcc ggtgcgatcc gaaatgaccg acgcgtaacg ggacagtcgc gtgcccgcca
    28441 tccacaccac aaagccggcc gcggccatta cggcgaaatt gagcggcagg ggttgcgcct
    28501 ggaaatcaat cattgcctta ctccaggctc gccatatacg cggcgaccgc ttgcagatcg
    28561 gcggccggca aactgttgaa cgccggcatg cgattgcccg gtttgagcgc ctgactgtcg
    28621 gcgatccagc cgcctagtgc gccgatgccg ttcggcagca accctgcgcc gatcgtgcgg
    28681 cggctgccca catgggtgag atcggggccc agcgcgccgc ccgtgctcgt gccgcgcacg
    28741 gtatggcagg tggcgcaacc ggaagtcaga aagacttcgc gtccgcgttg cagcagcggc
    28801 gtgtcgggcg cgcgtgcttc gttgccttgc gcgcgtcgcc actgctcgaa ggcgtcggcc
    28861 gcgacgactt ccaccacgaa ggccattttc gcgtgctgtg tgccgcaata ctcggcgcac
    28921 tgaccgcggt actgacccgt gcgttccgca tgcacgtgca tggtcttggg atggccggga
    28981 atcatgtcga gcttgccgtg cagattcggc acccagaagc tgtggatcac atcggcggtg
    29041 cgcaagtgga atgcgacagt ggtgccggcc ggaatgacga tttcgttcgc tgtttcgaac
    29101 tgcgccttgc ccgccgcgtc gagataacgc acgcgccacc agaattgttc gccggtcact
    29161 tcgatgtgca gttcgggttc cccgcgcaac agcagattgg gcgcgccgac gaaggcgtag
    29221 atcatcaacg ccgtcaacgt gacggccgga aagacgatgc cgccgccgat gatgagattg
    29281 cggtgcgcga gcgggttgcg tccgcgcggt ccgaaaatcg cataggccgc gagcgccatc
    29341 accgtgacga agatcagcgt tgcgccgcag tagagcagcc atgacaaatt gctgatgaac
    29401 gccgcttcgc cgctgaccgg atcgagcgcg tgttgcatgc cgcgacagcc ggcggcggga
    29461 acgagcagca gcagaatgag cgtgcgtgcg cgcctcattt gagcgtttcc agaaacgcgg
    29521 cgagatcgtg cgcgtcgcgt tcggtcacgc cgagattggg catcgccgtg cgcggatcga
    29581 cggcctgcgg gtcgcgcagc cagcgcacca tgttggcccg cgtgttgggc agcacgccgc
    29641 cgatgaagcc gcgcgtcgcc atgcccttca gcggcgggcc gacgggcgcg atggcgccca
    29701 ccacgtcggg aatttcgtgg caggtcacgc aggcgtattg ctgcatggcg atggcgccgc
    29761 gttccgcatt gccttcgaaa ggatcgggct tggctgtgat cgcgtcttcg cgctgttgtg
    29821 tctgcgccgg tttgagcgga ccttcgccga tggctttcgc gcgtgcctgg tagtcgagcg
    29881 gcgtcatctt cggcatggcg accagaaagg cgatgacgtc ccagatctgt tcgtcgggca
    29941 gacggaattc ccacgccggc atgcccgtca tcttgatgcc gtagcgcacc acccagtaca
    30001 tctcgcgcgc gggccaggtc ttcgccgtgt gcgcgagatt cgccggcagc ggtgtcatgc
    30061 cgagtgcgaa ggcattgggc ggcacgcccg gcgcgccatg acagttcatg caatgctcgc
    30121 gatagagcac cagcccgttg gcgatgcgtt gcggatcgtc cagcggcggc ggttcgatgt
    30181 ctttcgcgcg caccgctgcc gcgcgtttga gcgaggcgtt gagcagccaa tagaccggcg
    30241 ccgtgtgctg ctgtgtcgcg gcgacgttgt aaacgccgaa ataaaccatc gcggcaccgg
    30301 cgagcgcgcc gagtattccc agcgcgagta gaagcttgat ggcaccgcgc aagctcatgg
    30361 cgtcatcctc ggcgatgcag gaacgcgacg atgtcgcgcg cctgctcttc ggtgacgccc
    30421 aggttcggca tggcggtcag cggatcgacc tcctgcggca cgcggatcca gcgcaccaga
    30481 ttctccggcg tgttggccag cacaccggcg atgtagacgc ggtgcgcgat gccgtcgagc
    30541 ggcgggccga cttcgccgac cgcgccgacg acgccttcga tccgatggca actgccgcaa
    30601 ccgaattgcc agagcagttc gcggccgcgt tccggatcgc cagcggccgt atgggattcc
    30661 gacgagtcgc cgcaggcggc caacgagagg cagcacagca gcgcgccggc gaggcgcagg
    30721 ggcgagagcg ggaagagggc ggacatcgct cctctccaag aaaggaacgg acccgctttt
    30781 gcgcgcacca cgggcatgcc gattgcggct tgcgcgctgg tccatgtccg ttggagcgat
    30841 gcttgcgcct gcgttttgcc ttacttgtgc tggcaccctg gttcgcggcc ggagcgtggg
    30901 cgcatgatgt cagccgcatc gcggcgtcga ccgtgccgcc ggacccgcat ttgagtttcg
    30961 atccctggac cggcgtgccg ctggttgtca gcgcgctgct ctacggctgg ggcgtgatca
    31021 ggctgtggcg ccgcgccggt cgcgggcgcg gcatcagtta tgtgcagctc ctgtacttcg
    31081 tcgcaggttg gctggcgctg gccgttgcgt tgttgtcgcc catcgagacg ctcggcacct
    31141 attccttcgc ggcgcacatg ctgcagcatg aattgctgat ggcggtcggt gcgccgctga
    31201 tggtgttgag tcggccgctg gccgcctgga cctgggcact gtcggcagaa gggcggcgca
    31261 gcgcggggcg cggcatgcgt cacccccttt ttgctgcggc gtggggtttc atcactgcgc
    31321 ccgtgccggc ctggggtctg cacgctgtcg cgctgtgggc ctggcacgcg ccggcgctgt
    31381 tcaacgccac gctggtcgac gataccgcgc ataccttgca gcacatctgc tttctcgcca
    31441 ccgcgctgct gttctggtgg accgtgctgg ggcgcgatgc gcgcgtcgcg cccggcgtac
    31501 cgattctgtc gctgctcacc acgctgatcc acaccggcat cctgggggca ttgatcacct
    31561 ttgcgccgag cgtgtggtat tcggtctatc tcacgcgttc gccgctgctg ggtctcgatc
    31621 cgctggtcga tcagcaactg ggcggcctga tcatgtgggt gccggccggc gcggtgtatc
    31681 tcatcgcggc gctggcgctc atgctgcgct ggctgcgccc cgcattgacc gggcgtacgg
    31741 caacgttgcg ctgagcgcct agtcgggtcg ctgttcctcg tctgcgccgg cgtcgcgcaa
    31801 gctcactttc gtttcgccgc cgtgctccac gtcgtgctcc tcgtgcgact cgcggaacat
    31861 ctccttgcgc gcgtgctgct gcgcctgcgt gcgctccgcc ttggtttcct tcgcttcgcg
    31921 ataggcgtgc gtcttgaaat acaccagtgc cagggtggcc agcgcgagcg cgccggccat
    31981 catgagcaga tccaacgtct cctcatgctg gatgaagcgc tgcaggaagt ggaccgccat
    32041 aatgacgatc accacgctga tgattttcgt ctccagatcg ttgagcgtct cgatgcccat
    32101 ggcgatggtg agattgagcg gcgaaatgaa caggctgtag aagcccacgc cgatgagata
    32161 gaacaccacc gccttcagca tcacggaaac gatttccagg aagcgcaccg tgaggtcggt
    32221 cggcccgatg gtttccgcga acacgccgta ccaggcttcg atgacggtct tgaaggcgat
    32281 gatcgcgccg aggaggaaga gcgagaaggc ggccagcagc acggcgatga cggcgacgat
    32341 gacgataagg cgcgagcgcc cgatcgtctt ggcgaggaag gaatcgtggc cgttgtcgtt
    32401 cggtttcaca tcgagtgcgg tggcgtgccc gatccggccg ccacgcaagc ggcgcgcctg
    32461 accggccggc ggttcatgca tttgtcatat gtccgacagc tcggtgcgcg aattcgcgcg
    32521 cggacgcgac ataatccccc cgtcgtgaca accggcaagg tgcgtgctcg gcctccggcc
    32581 tctatggttg ccggcattcc ttaccttaga acggaaagtg ctttccatgg acagcatctg
    32641 gatgatctgg ccgggcaatc ccggcctgtc tgccgccacc tggctgctgg gcgccgtggc
    32701 gtttctttat gcggcgcgca cgtcggtgca cgaactcgtc cgctcgacgg gtcaggccat
    32761 cgccggcccg ctgcgcgcga tctcgcgctg gctcagtgcc gcggccgacg aactgcatgc
    32821 acgcaaccgc gcggtgctgc tcggacaggc gcgtcgcgaa gtggaagccc gcatcgagcg
    32881 cgaattcgaa cgcgtgagcg ggctggtgac gcgcgacctg gacggctacc cctcgctgca
    32941 gcgcaagctg caggacgaaa tcgcgtgcat ggaggaggac tacaagaagt gcggcgaagt
    33001 gccgccggcc tcgcccgact gggtggaggc ggtcgccgcc gtcgcccggg tgaagtcggg
    33061 caacgagatg gtgcagaaga tcctcaccga tatcagcacc tcgatcaacg tcatgcacga
    33121 caagtcgctg gccgagtacc gccgcgccta caagtcgcgc catcgcatcc tgggccggtt
    33181 cctgccgttc tggcgttcgg tcgagcgcac cctgtcgcgc gtggaccaga agctggaggg
    33241 actgcggtcg agcgcggcca tcgtggacgc gcaaatggac aagtacggcg aaatcactgc
    33301 caacagcgac cgcgtggcgc atgcgctcac ggtgtcggcc ttcacgcaac tcgtggtgtc
    33361 gctgctggtg ctggcggtgg ccgccggcgg ggcgttcgtg aactttaagc tgatcgcact
    33421 gccgatgtcc gaaatggtcg gcgcgggcga catcctcgcc ggcaatctgc gcacctcgga
    33481 agtcgcggcg ctggtcatca tcctggtgga aacctccatg ggcctgttcg tcatggagac
    33541 gctgcgcatc accaacctct tcccgcccat cgcgcaactg gacgatcgta tgcggcgtcg
    33601 cttgctgtgg gtgtcgggta cgctgctgat cgtcctcgcc gcggtcgagg cctcgctcgc
    33661 gctcatgcgc gacatgctga tcgccgacaa gcaggcgctg atccagtcgc tggccctggg
    33721 tgccgagcag gtgatgatcg cggacaagtg ggtgggcaat atccccacca tcgggcagat
    33781 ggtgctcggt ttcatcctgc ccttcgcgct ggccttcgtc gccattccgc tggaatcgct
    33841 cattcattcc gcgcgcacgg tgggcggtgt gatcgcactc gcgttgatgc ggctgctcgc
    33901 ctttgtgctg cgcctggtcg gccacatcgc gaaacacacg agccgggtgc tgatcacgct
    33961 ctacgatctg atcatcgtgg tgccgctgat gatcgagcgg ctcggcaagc gcgccgtggc
    34021 cgaggtctcg ccacctgtcg cgcccgccct cgaagccgag gaagggagga gggcttcgtg
    34081 agaatcgtgt tcagccatct ggcgccgctc ctgcttgcgc tggtcgtggc gctgctcgcc
    34141 ggctgcggcg acgcgcgcaa ccacgcgcat gcggtgtacg tgctcatcga cacctcgggc
    34201 acctacgcgc aggaagccgt caaggcccat cgcatcgtca actatctgct cggcacattg
    34261 aatcccggcg actcgctcgc cgtcgcccgg gtggaaagcc gcagcttcag cgagaaggac
    34321 atcatcgcca aggtcacgtt cgatcagcgg ccctcgcagg cgaatgcgca gaagcgcgcg
    34381 tttcaggcag cggtcgatgc ctttctcaag ggcgttcagg gcagtcgcta cactgacatc
    34441 accggcgggc tgctgcaggg cgcggaatac ctgaacgaga gcggtgccgg caccaaaacg
    34501 cttatcgtgt tttccgatat gcaggaagaa ctcgatcaga acaccgtccg ggactttccc
    34561 atcgacatga ccggcattcg cgtaatcgcg gtcaatgtgg tcaagctgcg cacggacaac
    34621 gccgatccgc ggcgctactt cgggcggctg gaagcctggc agcaacgtct ggaagacgcc
    34681 ggcgcgaagg agtggcgggt gatcaacgat ctcgaacggc tcgacccggt actggcgcga
    34741 cgctgaggcg ccgcgccgtt ccgggaacgg cggctgcagg ggctgcgcat cacgattgcg
    34801 gagatcccca tgcggcaacg attgtcgatt ttccggggac ggcgatgagc gtcgccgtcg
    34861 tcaccggcgc ctcgggcggt atcggccgtg ccacggcggt ggcgttcggc agacgcggct
    34921 ggcgcgtcgc gctgctcgcg cgcggtgccg aaggcctggc cggtgcgcag cgcgaggtgg
    34981 agcaggcggg cggcgaagcg ctgatcattc ccaccgatgt cgccgatcag tccgcggtgg
    35041 aagcggctgc cgcacaggtc gagcgcgagt ggggcgctat cgacgtgtgg gtcaacaacg
    35101 ccatggccac catctttgcg ccggtcatgg cggtgcaccc cgaggacttc aaacgcgcga
    35161 ccgaggtgac gtatctcggc tcggtatggg gcacgctcgc ggcgctcaaa cacatgatgc
    35221 cgcgcgaccg cggcgtcatc gtgcaggtcg gctcggcgct cgcgtatcgt tccattccct
    35281 tgcaggcggc gtattgcggc gcaaaaagcg cggtgcgcgg tttcatcgat tcggtgcgct
    35341 gcgaactgat ccacgaacgc agcaacgtcc gtctgacaca cgtgcatctg gccgcattca
    35401 acacgccgca attcgattgg gggcgcacca cgctgcggcg caagcccatg cccgtgggca
    35461 agatcttcca gcccgaagtg gcggcggaaa gcatctaccg tgcggcgcaa catccgcgcc
    35521 gcgaagtatg ggtaggctgg cccgcggcgc aggcgatcct gggtcagaaa gtgctgccgg
    35581 gctatctcga tcgccgcctc gccttcgaag cctgggaagg ccaactcgac aacgagccga
    35641 ttccacccgg gcgcgtggac aatctctacg cgccggtgcg ggaagatcgt ggcgcgcacg
    35701 gccgcttcga cgcgcgcgcg ctgccgcaca gcatgcaggc gtggctcaat cgcaatcgtg
    35761 tcgccgtcgc gctggggatc gcgctgccgc tgctggcttt gggtgcaatg cggcggcggt
    35821 aggtgaacac gccggtgttt tcggatgtct cgtcaacggc ggactgaatt ccggtccgcg
    35881 ctgcgcgcgc tcaccacgaa cggcttgcgg gccgcgcgtt ctgtgcgccc ggctcttcgc
    35941 tctcgactct cgactgcttc tctaaaactt ctcgctccag tgcaaatacc gccacttgcc
    36001 gggcggcagt ttgcccagcg caatgcggcc gatgcgcacg cgtttcaatc ccacgacctt
    36061 gaggccgacc gcctcgcaca tgcggcggat ctgccgcttc ttgccttcgc gcagcacgaa
    36121 gcgcaactgg tcttcgttct gccaactgac tttggccggt tgcagcggaa cgccgtcgag
    36181 ttccaggccg tggttaagca gcgccagcga cttggcgggc agcttgccgc ccttcgtgta
    36241 ttcgacccgg accaggtatt ccttctcgat gtcggagcgc tcgccgatga gttgtttggc
    36301 ggtgcggccg tcctgcgtca gcaccagcag gccgaccgag tcgatgtcga gccgcccggc
    36361 gggggcgagg cccttgaggt gttcgcgctt gaacgactgt ttggtcggat cttcggacca
    36421 gtggttttcg ggcgtgatga gggtgattgc cggtttgtag ccctgttcgg cctgtccgct
    36481 gacatacccc accggtttgt tgagcaggat ggtgacgcgc gctgcctgtt cgcgcgtggc
    36541 gcgtttgtcg attgtaattt tttgatgcgg caggacgcgg gagcccagct ccgacacgac
    36601 ttcaccgtcc acacgcaccc agccgcgttc gatccattcg tccgcttcgc ggcgcgaggt
    36661 caggcccagt tcgctcatgc gcttggacag gcgcacgctg ccgggcggat gggcgcgagg
    36721 cggtgctgcg ggagtcgctt gcgctgccgc acgcggggcg gtggggcggg gcgaagcgcg
    36781 cttcgctccg gcacgcggtg gcgtcgtttc ggcggggcgc cgcgcggtgc ggggcgtgtc
    36841 gcgcggggtc ttcgttgctt cgtttgcgcg cgcagctttt ttcggtttgg cgggcggcgc
    36901 ggtctctgcg gtcgtcgcgg gctgtgtgcg gcgcgcggtg ggtgcgcgtc gtacgggcgg
    36961 gcgttgcgga tcggcgcttg ccggtttgcg taatttgagt gtggccattg cgggcgcgat
    37021 attgcggcac ccaggcaggt gcggcaagca tttcgcgtat tgcgggggcg gggcgccgat
    37081 gctatcgtcg cgccacgcgg cgattcgtcc ctacaaaacc aaaagcagac gccgcgcaaa
    37141 tgcagcgccg tggggatgaa tgtctgcaat gcctatgtaa ttagcctgtt cggcggattg
    37201 accgccgcgg gccggctccc tacggttgcg caacagtgtc aatgcggttt ttagggagat
    37261 gaaatgggcc agggtgacaa cggcggcttc cccgccttgt tcgacaacga cgatctggat
    37321 cgcctgcgcg ccgagcaact cgagcgcgaa tatccacgtt ccgagcctta ccggcccttg
    37381 ccgatcgtcg cggcccatta tccgcgcatt gccgaagcca tccgccagct ttgggggacg
    37441 cccgaaggcg atgccttctt caatcgcatg ctgatcgacg aacgcggcaa ccgcgcgggc
    37501 ttcccgcccg atgtggtgcg tgcgctgctc gcgctgtccg aactgcatca gcaggccttc
    37561 cgcttccgca agccggaaga catctggatc gacgaccccg ccgtcgggcg tttcaacggc
    37621 cgctgatttc ccgccgccgg gaggcgggta cccggtctcg cacgcacagg caatcccggg
    37681 tataaaagcg tcatgagcaa gctgttttcc cccttcaggc tgcgcgacac gctgttccgc
    37741 aatcgcgcct tcgtctcccc gatgtgccag tactcggccg aggacgggct gcccaacgag
    37801 tggcattacg tccacctggg cagccgcgct gtcggcggtg cgggactggt gatggtggag
    37861 gcgaccgccg tgtcgcccgc cgggcgcatt tcgcccgggg acaccggcat ctggaacgac
    37921 gcgcagcgcg accgctttca gcgcatcagc agcttcatcg aagcgcaggg tgcggtgagc
    37981 gccatccagc tcgcgcacgc cggccgcaag gcgtccaccg ccgccccctg ggacggcggc
    38041 gcgccgctcg agcgcgagca gggcggctgg gattgcctgg ctccgagcgc gctgcctttc
    38101 gacaaaaact ggccgacgcc catcgaaatg acgaaggcgg acatcgatgc gacggtggag
    38161 cgcttccgcg aagccgcggt gcgcagcctc gatgccggtt tccaggtcgt ggaagtgcac
    38221 atggcgcacg gctatctgct gcacgagttc ctgtcgccgc tggccaatcg tcgcggcgac
    38281 gaatatggcg gcagcctgga aaaccgcatg cgtttcccgc ttgcggtcac gcgcgccgtg
    38341 cgcgaagtgt ggccggagca ccggccggtg ttcgtgcgca tttccgcgac cgactggctc
    38401 gacggcggct gggatctgga acagagcctg gtgcttgcgc gcgagttgcg caatctcggc
    38461 gtcgatctca tcgactgctc caccggcggc gtcgcgcccg gcgcgaagat acccgtcggc
    38521 cccggctatc aggtgcgctt cgccgaaacc atacgcgccg aggtgggcat tgccaccggc
    38581 gcggtggggc tgatcaccaa accgcgtcag gcggagcaga tcgtcgcgac cgaacaggcc
    38641 gacgtggtct tcctcgcgcg cgaattgctg cgcgatccgt actggccgtt gcgtgccgcg
    38701 catgcgctga aagcggatgt ccattggccg aaacaatacg aacgcgcccg acccgccgac
    38761 ctggagtaag cccgcgccca tgacagccga ccgccacgcg ctgttcaccg atctgtacga
    38821 actcaccatg ctccaggcct atctggaaga gggcatggag aaggaggcgg tgttcagcct
    38881 gttcgtgcgg cggttgccgc agcggcgcaa ctacctgctc gcctgcggac tcgaaacggt
    38941 gctcgattat ctggagaacc tgcgcttcga cgacggcgac atcgcctatc tgcgcagtct
    39001 cggcaagttc agcgaccgct ttctcgcatg gctggcggac ttccgcttca ccggcgaagt
    39061 gcatgccgtg ccggaaggca cggtggtgta tgcgaacgaa ccgattctcg aagtcatcgc
    39121 gccgctgccg caggcgcaac tggtcgagac cttcatcatg aatcaggtgc actaccagac
    39181 catggcggca tcgaaggctt cgcgcgtgat caccgctgcg cgcggccgta gggtcgtgga
    39241 tttcggtgca cggcgctttc acggcaccga tgcggccatc aaggccgcac gcgctttcta
    39301 catcgccggc gtggcagcga cctccaatgt ggaagccggg cggcgttacg gcattcccgt
    39361 cgccggcacc atggcgcaca gctacatcca ggcgcacgag ggcgaagtcg ccgcgttccg
    39421 cgcgttcgcg cgcctttatc cggacacggt gctgctggtc gatacctacg acacgctcgc
    39481 cgcggtgcgt aaggtcatcg acctggcgcg cgaatcgggc gacgcattcg aggtcaccgg
    39541 cctgcgcatc gattcgggcg atctcgtcgc gctgtcgcgc gaagcgcgga gactgctcga
    39601 cgaagcgggt ctgcagcacg tcgaactcgt cgccagcggc aacctcgacg aggaccgcgt
    39661 cgcggcgctg ctcgaagccg atgcgccttt cgacaccttc ggcgtcggta cggcgatggg
    39721 cgtgtccaac gatgcgcctt cgctcgatat cgcctacaag ctcaccgagt acggcaaacg
    39781 cggccgcgtc aaactctcgg ccgacaagcc ggtgtttccc ggacgtcatc agatctacag
    39841 aatggaaagc gacggttacg acgtgcacga cgtcatcggc cgcgcgcagg agacgctggc
    39901 gggccggccg ctcctcgggc ccgtcatgaa aggcggcaag cgtctgtcgg atgcgacgcc
    39961 gccgctcgac cgcgtgcgcg aacatgcgcg cgagcaagtg atgcggctgc cggagcatgt
    40021 ccgtcggctg tcgcccgccg aacccccgta ccccgtgcag ttgagcgacg cgctcactcg
    40081 ctatcagcat cgggtctcga gaaactttct gccctgacgc gtttgcgccg cgcgggcttc
    40141 attccggccc gtcgcggcga agcgggcggc caggcgccgc aggcgtcatg tggcaaagga
    40201 gaagcggcat gagccatccc accgaaatgc tgcaacccgg cgatgccctc ctcatcgtcg
    40261 acgtacagaa cgacttctgc ccaggcggtg cgctgcccat cgaacgcgga gacgaagtcg
    40321 ttcctgtgct gaaccgctgg atcgacgccg cgcgggccgc cggcgtgccg gtgtatgcct
    40381 cgcgcgactg gcatcccgcc ggacacctca gcttcgagga acgcgaaggt ccgtggcccc
    40441 cgcactgcgt gcaggatacg gcaggcgccg ccttccaccc cgacctgcgc ctgccgcccg
    40501 acacgatcaa gatcaccaag ggcgcgcgct tcgatcagga ccagtactcc gccttcgacc
    40561 agaccggcct tgccgatcaa ctgcgccgcg acggcatcaa acgcgtctgg atcggcggcc
    40621 tcgcgctcga cgtgtgcgtg cgcgccaccg tactcgacgc gctgaaggaa ggtttcgccg
    40681 cgcacctcat tcccgatgcc tcgttgccgg tgacgcgcga aggcggcgag aaagcgctgc
    40741 gcgagatgcg ggaggcgggg gcggagatat aacctgaagc aagggggcga gggagggagg
    40801 catgaaacgg cttgcgttga tgttcgtgct gcaattgttc tcgcaattgg cagttgccgg
    40861 gaacattgcg gacgtttttt ccgaagggat cttcggcgtc aaatggggaa gcgctataga
    40921 agaggtagag aaggcttttc ctggcggagt gaccgaaacg caggtcggta ttacaacgta
    40981 cgcaatccag gatggccgca cgctcttcgg tgttcagcgc gaagacagca attcaataag
    41041 gttctcgttc gattccaacg gcgagatgaa cggtgtatcg atcgagttcc cgttaaatgc
    41101 tattgacgat tacggtgttc tgtttacgaa actcaatacg gtattcggtc gcggcgagga
    41161 cgctccaaac tcactggggg tgcatgtggt gaaatggcct gaagaccaag gaataaccat
    41221 tagccttatg catctcccgg gcgttctcgg catcggcgat ctcctgttcg gtatcgagca
    41281 tcggcccgag ccctctggtg tgtcgaaagg cgatctcgga ttttgagtag accttcggaa
    41341 cccaggctgc gaggcccatg atccccgctg acgtgctgct cctcttcgtc gctgcctcgg
    41401 cggccctggc gatcgcgccg ggtccggaca acatttacgt gctcacgcaa tccgcggcgt
    41461 acgggaaagg gacgggaatt ctcatcacgc tgggcctctg caccggcctg ctgtttcaca
    41521 ttgccgcggt tgcgctgggt atggcggcga tactgaagac ctccgctgtt gccttcaccg
    41581 tcatcaagat tctcggcgcg gcctatctgc tctatctggc atggcaatcc ttccgtgccg
    41641 gcagcgcaac gattgcggcg tcgggcgagc gtccgctcgg cgcaagggcg ctctatctgc
    41701 gcggcatact gatgaatgtg agcaacccga aagtcgcgat cttctttctg gcctttctgc
    41761 cgcagtttgc cgatccggcc cgtggcgcgg tttccctgca gatcgtcaca ctgggtttgg
    41821 cgttcatggt cacggcattc gtcattttct gcggcatcgc gtgggcggca gggcggatcg
    41881 gcgagtggct caaaggctcg aagcgtgtac aaatcggcat ccaccgtctg acgggcgtgg
    41941 tatttgtgct gctcgcctgc cgtctcgcat tgagccggca ctaatcgtcg cccgcctgag
    42001 cggcgactcc gttcaactct cttttcctga ccatgttcaa gaacgcagtt cgtgcgctcg
    42061 cggtggtgct cgtgtgtggg acggcaagcg cggcttatgc gcaatctccc ggtcagccga
    42121 tcccgccggc gcattttttc gacggtccgc tcttgcacgt ttattcgccg gactcggaag
    42181 ggtggctcgt caccgggcgt agcgggatcg ggatcgcctt cggcaaaagg gggacggccc
    42241 ccaacgaaac cttcggtgcg caagccgtca tgttcgaact gggtcccgag gtgcgcggcg
    42301 acgcgctggt cgatttcgtc cgcaaacgga tcgcaaccat gaatccgccg ccgcggttcg
    42361 aggaaacgac atcggcgtac cggtatcacg aggcacgcgg ttatccctgc gtggatgtca
    42421 acgtcggatt ccacgacaac gccgccgtca cgccgagcgg cgagcagcgg ctgctgttgc
    42481 gcgtgattgc gctttattgc cggcaccccg tcgtgtctga actgggtttc ttcgcggcct
    42541 attcgcatcg cgggccgtcc ggcgatgcgg agatcaaaga ggcggcgatg cgttatatcg
    42601 ccggcgtcac cgtcaatccg cggatcgcgg agcaggtaaa acgtgaacct gccaggtgac
    42661 atcgaacagc aggtgctcga gtagctgctg ctcgaattcg gagaagcgcc ggatgacccc
    42721 gacgcgctgc tgcagttgag cgatattgac tatcgggcgt gcatgagatc ggcgacgtgc
    42781 cgaccgactg tgaaggtgga agcgaaccgc caattgctcg accggatggc ggccgaagtg
    42841 ttgcgcgaat ccgccggctg agtcctatcg ggagtcggcc ggcaccagcc agacgcttgc
    42901 cgccgcaagc atcgcctcga cgccggcgcg caaggtcgga ttcggcacgg gagcgaactc
    42961 gggagagtga ttcgacggca gcgcgttcag gttgccggtc tttttggcct cggcgtactg
    43021 ctgcggatcg acgccgccga cgatccagta cacggatgga atcccccagg cgcggggaag
    43081 cagcgagaaa tcctcgctcg ccgaggccgg gccggtcgag ccgacgcgtt cgctgccgaa
    43141 acgctgctgc agggcggccg tgactttgcg cgtcgcctcc tcgtcgttga ccgtcagcgg
    43201 aaactcgccg ggcaaccggg tgatcacggg cggcttcggc gcgcctgcag tgcgcgcctc
    43261 gccctcgacg acgcgcttga tcgccgccag cacctgctcg cgcacttcgt cgtcgaacgt
    43321 ccggatgttg agttgcagga cggcttcgtc cggaatgatg ttcgcgctcg atccggcctg
    43381 caccgagccg accgaaagca ccgcgttgtc ggtcaagccg agttcgcgcg aaaccacggt
    43441 ctgcagtcgc atgatcgtcg ccgccgccat caccaccgga tcgacggtcg tctggggcga
    43501 ggagccgtga ccgccgcggc cgtacagcgt gacgcgcagg ctgtcgctct gcgacagcac
    43561 gaggccgttg cggatggcga tgttgccggc cggcagcggc aatacatgct gcgccagcgc
    43621 gatgtccggt ttgggaaagc gcttcgcgag cccgtcatcg agcatcgcgc gcgcgcccat
    43681 gccggtctcc tcgccgggct ggaacaccac catcaccgtg ccgcgccact tgtccttgtt
    43741 tgccgcgagg acttgcgcgg cgcccatcag ccaggccacg tgcatgtcgt ggccgcagga
    43801 atgcgcaata ccggtttccc ggctggatga aggatcgatg ccggtcttct tgctcgcata
    43861 gggcaggccg gtgttctcct gcatcggcaa cgcatccatg tcggcccgca acaggaccgt
    43921 tgcgccttcg ccgttacgca gtacgccgac cacgccggta ccgcccacgc cttccgtgac
    43981 ttcgtagccc tgttgtcgaa gccagtcggc ggcgatgccg gcagtgcgct tctcctgcat
    44041 agagagttcg ggattggcgt gtatgtcctt ttagaccgtc tcgagcttct tgaggagttc
    44101 actggacggg cttggaagcg cgggaccttg tccgaacgcc gacgccggtt ggagcaggac
    44161 caatacggcg atcagtgttg tgaaggacat cgatacctcg gcgatgagat tgcgcttgct
    44221 gacgaccatg ctggctctcc cggactacga acggccacag cgccgacgac ccgctgtgcg
    44281 ttgcagtctg tgtccagcaa tatggagtcc caaggatcgc gagatggtga agcgtgcggg
    44341 gcgtcacgca agcgtgccct gcgcttgcgt ccaagccgaa gcgcagaaaa acgcaaaaat
    44401 catcaaagca tccggtgcgg tcggcgattg accaatcgtg gtgggcgctg cacacaacca
    44461 acgcggtcga gtatggatgg cgttcgtaag ctcatacgag ctgttcatct ttcagtgcgt
    44521 ggcgcgccag tcgttcgttc ttccgcagca ggcgatcgaa cagccggggc catagcgcgt
    44581 tcagccgggc gtagagcggt tccgcgttac caaaggttcg ttcggcgata tccgcttcga
    44641 tggctttcac aatattcgtg gcgacgacgt cggcatcgtc gaacaccatg tgcgccgctt
    44701 gtgcatatcg atgcacggcc gcgctgttca tgggcgttcg cgttgcacgc ggggccacgt
    44761 aggtcacgcc gatgccgcgg tcgccgagtt cccggcgcag cgcttcggag aagccgcgca
    44821 ggccgaactt ggtcgcggaa taggcggtga agtagggaaa gccgatagta ccgaagacgg
    44881 acccgacgtt cacgatgcgt gcgcccgatc gcatatgggg aatcacggcc gcgcatagcg
    44941 acatcggtgc gatgaggttg atcgtgacga gccgttgaat ggtcgaggga tcgttctcat
    45001 ataacgcgcc gaagtgcgag accccggcgg cgttgacgag cagatcgata ccgcccaggc
    45061 ggcgaatcgc gctgccggcc aatcccgcgt atccgtccgt cgcggcgaga tcgaacagca
    45121 atgtttccgc gccgagtcgt gccgcgagcg gtgcaagctg ttcgcggtcg cgtccgacca
    45181 gcaccatgcg cgcacctctt tgcgccagtt gccccgcgag cgcgcgcccg atgccgcccg
    45241 ttgcgccggt gagcagtacg cgtgtgtcct gaagtttcat gccgcctcct tgaccaccgt
    45301 cccggcgcgc cgggcgtcga ggtggcggaa gatgtcgccg tagagtttgt agaacatgcg
    45361 tgcgctgtgc gcgattgcgg agcgatcggc cgagtcatcg agacgatcga cgagcgaggc
    45421 gtagtacttg atgtgtccct gatcgaggct gccgtgagaa gtcagatagc tgaacgcctt
    45481 ggccggcaga gcgagcgagt cgcgcagcgc ttcggctgct tgcgtggcga cggtcacgct
    45541 cgtcccctcg aggaccagaa ccatgccgag aaacgcggcg gggttacggc gcgcgatgag
    45601 atcgtaggcg taggcgacca tgagctcagt ggcttgcgac ggcatcccgt gtcggacgct
    45661 ttcggcatcc gcgccgcagg cgcgcagatc gttgagaatc cattcctggt ggccgacttc
    45721 ctccgcgacg tactccgaca tcgcctggcg caaccattcg agccgttcgg gcagtcgcgc
    45781 gccgcatgcc atgagcagcg gcagggtgtg tttcacgtgg tgataggcct cagcgagaaa
    45841 ggccgtgtaa tcggcgagcg acaggcggcc cgccgcgccg tcgcggatga atggaatggc
    45901 caacagctgt tcgcgatttg cttgcgttgc ggcgagtagt gcttcgtagt gcgtcatgcg
    45961 ttttccttgt aggtctcagt gtggcgggcg tctgcgtaca acgcttcgat ctgccgcccg
    46021 tgacgctgca gcaagacgtc gcggcgcagg cgtccgttgg cggtgatctc gccgctcgca
    46081 aacgtaaatg gcgtgtcgac cgtgatccag cgtcgcacct gggcgtagtc gggcagcatg
    46141 cgatttgcgg cggcgaccgc atcgtctacc gcctcgcggc tggccgagac gatgacggcg
    46201 gagagccacg gccggctctc accgaagacg gcagcctggg caatggccgg ttgcgcggtg
    46261 agttcgcttt ccacccactc cggtgcaacg ttgcggccga aggcggtcac gaacatgttg
    46321 cggcgccgtc cggtgagatg gacgtagccg tcatcatcga ggtaaccgag gtcgccggtc
    46381 ggccaggggc cggctggacg tgtgcctgcg ccgacgtatc cgagcgcggt cgcgcccgtg
    46441 acgtacaact cgtcgttccg cacctcgatg cgcgcatgag ggagcgcacg accgacgctg
    46501 ccgtccttgc aggcgcgggg tgtgttgaga gcgaccaccg atgcgcattc gcttaggccg
    46561 taaccttcgt acaccggcaa gccgcatcgg cgcgcgcgtc cgatcagcgt agccgacacc
    46621 ggggcgccgc cgacggcgac gaagcgcagc tgcggcaatc gcgcgcctgc gtcctccacc
    46681 gcgtccacaa gtccggccag catttggggc accagaatca ccgtgctggc cccatgctca
    46741 cgcagttgcg ccatcaattg atggccatcg aagcccgcca tgccgctcag gccgatgtca
    46801 ctcgag
//
LOCUS       DATGOU010000021        12863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_1383138, whole genome shotgun sequence.
ACCESSION   DATGOU010000021 DATGOU010000000
VERSION     DATGOU010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12863
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383138"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VKZ48_01280"
     CDS             complement(<1..512)
                     /locus_tag="VKZ48_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HLU76516.1"
                     /translation="MNLSKRLGAGALLLLAGCSLAPTYEAPQYELPAQYKEAAALPDA
                     ERGLWKEAEPAEHAVRGEWWRVFEDPVLDDLQTRAQAANAQLQAAAARVTQARAIAGI
                     TRADQLPQIGVGAGPSRQRPSAASLGLPDGADTSPQSVWRAQAFASYEIDLFGRVADG
                     AAAARLDAESA"
     gene            complement(521..3694)
                     /locus_tag="VKZ48_01285"
     CDS             complement(521..3694)
                     /locus_tag="VKZ48_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HLU76517.1"
                     /translation="MSPNLSKVFIDRPIFAGVLSTFIFILGVLALFKLPVSEYPDVVP
                     PSVVVRAVYPGANPAVIATTVAAPLEEQINGVENMLYMSSQATTDGVLTLTVTFKIGT
                     DVDLAETQVQNRVQRALPRMPEEVRQIGITTEKSSPNLTMVVHLLSPDERYDELYLRN
                     YAVLNVKDVLQRIPGMGQTLVFGGGDYAMRVWLDPQKVAARNLTASDVANAIREQNLQ
                     VAAGVVGAPPVADGTEFQLSINARGRLTTEEEFGNIIVATSPDGRITRLRDIARIELG
                     AGDYALRSLLNNKPAVAIGVFQAPGSNAIALSNEVRETMERLKADFPHGVDYDIVYDP
                     TRFVQKSIEAVIWTLLEAIVLVVLVVIVFLQTWRASIIPLAAVPVSIVGTFALLLWFG
                     FSINTLTLFGLVLAIGIVVDDAIVVVENVERNIELGYDPKEATYIAMREVSGPIIAIA
                     LTLCAVFVPIAFISGLTGQFYQQFALTIAISTIISAFNSLTLSPALAARLLRPHDAPK
                     DRFTRFIDRSFGWFFVRFNNFFRRSADRYEHAVARTLNRKALSFGIYAALIGATWFMF
                     QTVPPGFVPQQDKQYLVGFAQLPDGASLERTDAVIRRMSEIAMETPGIVHAVGFPGLS
                     INGFTNAPNAGIVFFTLDEFENRRSPELSGNAIAMNINQRLFEIQDAFIAVFPPPAVE
                     GMGTIGGFKMQIEDRAALGDEALYQAVQAMIGKASQTPELAGVFSSFQINVPQLQADV
                     DRTRIKQMGISLPDVFDTMQIYLGSLYVNDFTRFGRTYRVVVQADAPYRSDAQDIALL
                     KTRNQAGEMVPLGALMDVRQSYGPDRAMRYNAFPAADLNGAAAPGYSSGQAQAAIERI
                     ADEVLPRGITYEWTDLTYQEILAGNTAVFVFPLCVLLVFLVLAAQYESFTLPLAVILI
                     VPMCLLCAIAGVWLTGGDNNIFTQIAFFVLMGLACKNAILIVEFARELELRGHGVVQS
                     ALEACRLRLRPILMTSIAFIMGVVPLALSTGAGAEMRTAIGIAVFFGMLGVTLFGLFL
                     TPVFYVALRSLTRRKPRGEPAAGEVAA"
     gene            complement(3709..4872)
                     /locus_tag="VKZ48_01290"
     CDS             complement(3709..4872)
                     /locus_tag="VKZ48_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLU76518.1"
                     /translation="MPTYASSYSLIAVVALVLALQGCRDAASQADTGAPPAPPVTVAP
                     AEARNIVQSDIFTGRLAAVESVEVRARVTGYIEEVRFKQGAMVKKGDVLVVIDPRPFQ
                     ANLARAEAEVAAAKTRLELARKELARSEELVKVNATSRQELDQRAAAVQDAIAAQRAA
                     EAQANRLRLDLAYTRVTAPIDGRVGRIEITAGNLIQGDAPNSPLLTTIVATTPIYAEF
                     EVPERVYLAYGLNAPGKSMPVAIGLASDEGFPREGQLVFVDNHVDPATGTVRMRAVLD
                     NQDGALTPGLYARVRLSEAVQRNAVLVPDRAIGTDQDRKFVLVVGEDNKAMYREVKLG
                     RLMSNERVVEEGLQPGELIVVNGLQRVRPGSPVMPQRAGDPLPAAHRGAGKPA"
     gene            complement(4890..5690)
                     /locus_tag="VKZ48_01295"
     CDS             complement(4890..5690)
                     /locus_tag="VKZ48_01295"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLU76519.1"
                     /translation="MVPFVPAADIAAQDRSCTGFDRTLRMRVHRRGSGRSTPPALVYF
                     HPGRFVSGGLEEADAAARALAERYGVAVITPAYSLAVECPFPAAAEDAYTALRWVHDN
                     AGAGLWSAKRIAVIGHEAGGNLAAVAAMMARDRGGPHVVAQVLLSPMLDPSLTSCSMR
                     QAEEASRACGEAYHRYLPNTADRLHPYAAPGLCTRLAQLPPALILTADDDPLRDEAQH
                     YGAKLDAAGVHTRVARLDKAGWSAAAWREIGDFLEPLLAPARGTPLHT"
     gene            5803..6759
                     /locus_tag="VKZ48_01300"
     CDS             5803..6759
                     /locus_tag="VKZ48_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLU76520.1"
                     /translation="MDRLQAMEAFVHVVQTGSFTRAADRLRLPKATVTTWIQNLEARL
                     RVKLLNRTTRKVSVTADGAAYYERCLRILNEVEETESVLTRTATSPQGRLRIDVPVSF
                     GRYILIPALPDFCARYPDIQLEIGCTDRPVDLIEEGVDCVVRGGELSDVSLVARRIAT
                     LHFVTCAHRDYIMRHGTPRHPNDLVDHRCIGYFSGKTGKPCNWDFARDGERIEFDVQP
                     YVSVNDSDAYLSAGLAGLGIMQVSMIKVRHALDKGLLVEVLPEWKVDPLPVYVMYPPN
                     RHLSAKVRVFVDWVAALVEGRDEMPLLDEQLCLLRQPQNLAA"
     gene            6803..8398
                     /locus_tag="VKZ48_01305"
     CDS             6803..8398
                     /locus_tag="VKZ48_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLU76521.1"
                     /translation="MRYDALIVGGGHNGLTCACYLAAAGLRVRVLERRHVVGGAAVTE
                     EFHPGFRNSVASYTVSLLNPKVIRDLRLAEHGLRIVERPYSNFLPLDDGDALEVGGGL
                     AATQREVARFSARDAERLPAYYDTIERVADVLRDLVLQTPPNVGGGLHDLLGALRLGM
                     RMRGLDMAARRDLLDLFTKSAGEMLDWWFESEPIKAAFGFDAVVGNFASPYTPGSAYV
                     LLHHCFGEVNGKRGIWGHAIGGMGAITQAMAAEAQARGVEISTASAVSQVLTRDGRAT
                     GVVLEDGTELEARLIVSNLNPKLLFLDLIAPDALEEDFRTRIERWKCASGTFRMNVAL
                     SELPDFSARPGTQVQPHHRSGIVMAPSLAYMDRAHQEAREHGWSRAPIVEMLIPSTVD
                     DTLAPEGMHVASLFCQHFNPVLPDGRSWDNARETAADLIIDTVSRYAPNFKSAVIARQ
                     ILSPLDLERTFGLVGGDIFHGALSLDQLYSARPVLGHANYRAPIKGLYLCGSGTHPGG
                     GVTGAPGHNAAFEILRDWRAHKL"
     gene            8485..9786
                     /locus_tag="VKZ48_01310"
     CDS             8485..9786
                     /locus_tag="VKZ48_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HLU76522.1"
                     /translation="MFDATALIVGLVIGAGIFRLPSLVAANTANEWVFYGVWIAGGLI
                     SLIGALCYAELATTYPNAGGDYYFLQRAFGRGLSFLFAWARIAVITTGSIAVLGYTFG
                     DYASNLYSLGSNSSAIYAALAVLAFTAINVSGIRETRGTQNVLTTFEVFGLLAVVVTG
                     LLLIAPEQAAPAAAATEPKAWTAGIPLAILFVLFTYGGWNEGAYISAELKNRRDMVRA
                     LVVSLAIVTLLYLLVNIAYERTLGLAGMAASETIASDVLGRYFGDWGAHIISAIVAVS
                     ALTSINATIVVGARSNYALGRDWPIFGWLGYWDDKADAPRNALYVQCAVAVLLIVVGA
                     FGGKIETMVDYTMPVFWFFFLMTGIGLFVLRHKDPHTPRPFKVPLYPLTPLIFVATCA
                     YLFYSSVAYVKGGAQLGIAVLLLGGVLLLLNTRRAKAQPAQ"
     gene            9809..10366
                     /locus_tag="VKZ48_01315"
     CDS             9809..10366
                     /locus_tag="VKZ48_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLU76523.1"
                     /translation="MERRRFIKLLSGAAALGLAGRAAYAQPIPPGPGHHLDAPFVPTR
                     EEVVEEMLRVAGTGPEDIVYDLGCGDGRIPIIAAQKFGARGVGIDIDPERIHESRENA
                     REAKVTDRVKFIAEDLFLADISEATVVTLYLLPSVNVRLKPKLQKELKPGTRVVSHDF
                     GMGEDWPPERTVKLGGDIIHLWRIE"
     gene            10537..11088
                     /locus_tag="VKZ48_01320"
     CDS             10537..11088
                     /locus_tag="VKZ48_01320"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLU76524.1"
                     /translation="MPDEDFSLYWDEDDDFGLDNEPVFDEHVYPEYLFDVPYVPTSPE
                     LVDAMLRFAGTGPGDVVYDLGCGDGRIAIAAAEKFGARAVGIEIDEHLIAEARSNAAL
                     ADTEGRVRFVHADFHEAHIADATVVAIYLLPEVNLLLKPRFLQELRPGTRIVSNYFGM
                     GEEWPADRTTRVGQDLIQLWRVP"
     gene            11107..12057
                     /gene="gnd"
                     /locus_tag="VKZ48_01325"
     CDS             11107..12057
                     /gene="gnd"
                     /locus_tag="VKZ48_01325"
                     /inference="COORDINATES: protein motif:HMM:NF007161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HLU76525.1"
                     /translation="MRDTGLNTRTGSGMKLGLIGLGRMGANMARRLARTGIEVVGYDV
                     STEARAALGSETGVSLVDSIDAVVANLPAPRALWIMLPAGAITQEAIDRLATLAAAGD
                     LIVDGGNAYYKDSMRRAEALVERGIDFIDAGVSGGVWGLENGYTLTLGGEAHAVKRLE
                     PVARALAPAPDRGWLHCGPAGSGHFVKMVHNGIEYGAMQAYAEGFALLAAKSEFELDV
                     PQIAETWRHGSVIRSWLLDLTAEALAQKPDLDGIAPVVGDSGEGRWTALEGVELGIPT
                     PVTSLALMMRFATQGRNDYPAKLLALMRNAFGGHAITAKR"
     gene            12071..12562
                     /locus_tag="VKZ48_01330"
     CDS             12071..12562
                     /locus_tag="VKZ48_01330"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HLU76526.1"
                     /translation="MVVIVMGVSGAGKTTVGKLLAHRLGAHFLDADDFHPEENVEKMR
                     GGSPLAESDRAPWLAQLNKELRKHHLRGERVVLACSALRRSHREQLLEGLPAAQVVYL
                     RGSRALLASRLAQRKGHFMPPALLDTQFEALEEPRNAVTVDVSGTPEGVVEAICAALD
                     LKA"
BASE COUNT         2285 a         4156 c         4166 g         2256 t
ORIGIN      
        1 gcgctttcgg catcgaggcg cgcggccgcc gcgccgtcgg caacgcgacc gaagagatcg
       61 atctcgtaac tggcgaaggc ctgcgcacgc cacaccgatt gcggcgaggt atccgcgccg
      121 tccggcaggc cgagcgatgc cgcgctgggc cgttgacgcg acggtcccgc accgacgccg
      181 atctgcggca gttgatccgc gcgcgtgatc ccggcgatgg cgcgtgcctg cgtcacgcgt
      241 gcggcggccg cctgcaattg cgcattggcc gcttgcgcgc gcgtctgcag gtcatcgagg
      301 acgggatcct cgaacacacg ccaccattca ccgcgcaccg catgctcggc aggctccgct
      361 tccttccaca acccgcgctc ggcgtcgggc aatgcggccg cttccttgta ctgcgccggc
      421 agctcgtatt gcggggcttc gtaagtcggc gcgagcgagc agcccgcaag cagcaggagc
      481 gcacctgcac ccagccgctt cgagagattc atgtgcacgc tcatgccgcc acctccccgg
      541 ccgccggctc gccgcgcggc tttctgcgcg tgaggctacg caaagcaacg tagaacacgg
      601 gcgtcaggaa caaaccgaac aaagtgacgc ccagcatgcc gaagaacacc gcgatgccga
      661 tggcggtgcg catctcggcg cccgcgccgg tggacagcgc cagcggtacc acgcccatga
      721 tgaaggcgat ggaggtcatc aggatcggac gcaaccgcaa acggcaggct tccagcgccg
      781 actgcaccac accatggccg cgcagctcca gttcgcgcgc gaactcgaca atcagaattg
      841 cgtttttgca ggcaagaccc atgagcacga agaaggcgat ttgagtgaag atgttgttgt
      901 cgccgccggt cagccacaca ccggcgatcg cgcacagcag acacatgggc acgatgagga
      961 tcaccgcaag cggcagcgtg aagctttcgt attgcgccgc gagcaccaga aacaccagca
     1021 gcacgcacag cggaaacacg aacacggcgg tattgcccgc gagaatctcc tgataggtga
     1081 gatcggtcca ttcgtaagtg atgccgcgcg gcagcacttc atcggcgatg cgttcgatcg
     1141 ccgcctgcgc ctgacccgag ctgtagccgg gtgcggcagc gccgttgaga tccgctgccg
     1201 gaaaggcgtt gtagcgcatc gcccgatcgg gaccgtagct ctgacgcacg tccatcagcg
     1261 cacccaacgg caccatttcg cccgcctggt tgcgcgtctt cagcagcgcg atgtcctgcg
     1321 catccgaccg ataaggcgca tccgcctgca ccaccacccg ataggtgcga ccgaaacgcg
     1381 tgaagtcgtt gacgtagagc gagccgaggt agatctgcat ggtgtcgaat acgtcgggca
     1441 gcgaaatgcc catctgcttg atgcgcgtgc gatccacatc ggcctgcaac tgcggcacgt
     1501 tgatctggaa gctggagaac acgcctgcca actcgggcgt ctgagacgcc ttgccgatca
     1561 tggcctgcac cgcttggtag agcgcctcgt cgccgagtgc ggcgcggtcc tcgatctgca
     1621 tcttgaaccc gccgatcgtg cccatgccct ccaccgccgg cggcgggaac accgcaatga
     1681 acgcgtcctg gatttcgaac agacgctggt tgatgttcat cgcgatggca ttgccggaca
     1741 actcgggcga acggcggttc tcgaattcgt ccaaggtaaa gaacacgatc ccggcgttgg
     1801 gagcgttggt gaaaccgttg atcgacaggc cgggaaaacc gaccgcgtgc acgatgccgg
     1861 gcgtctccat ggcgatctcg gacatgcggc ggatgaccgc gtccgtgcgc tccagcgatg
     1921 cgccgtcagg cagttgcgca aagccgacga gatactgctt gtcttgctgc ggcacgaaac
     1981 cgggcggcac ggtctggaac atgaaccacg tcgcgccgat gagcgcggca tagatgccga
     2041 aggacagtgc cttgcgattc agtgtgcgcg caaccgcgtg ctcgtaccga tccgccgacc
     2101 gccggaaaaa attgttgaag cgcacgaaga accagccgaa ggaacggtcg atgaagcgcg
     2161 tgaagcgatc cttcggtgca tcgtgcggac gcaacagccg cgccgccaat gcgggcgaca
     2221 gcgtgagcga attgaacgcc gagatgatgg tggagatggc gatcgtcaac gcgaactgct
     2281 gatagaactg tcccgtcagc ccgctgatga acgcgatggg cacgaacacc gcgcacaagg
     2341 tgagcgcgat ggcgatgatc ggaccgctca cttcgcgcat cgcgatatac gtcgcctcct
     2401 tcgggtcgta acccagttcg atgttgcgtt cgacgttctc caccacgacg atggcgtcat
     2461 cgaccacgat cccgatggca agcaccagtc cgaacagggt cagggtgttg atcgagaatc
     2521 cgaaccacaa tagcagcgcg aacgtaccga cgatggaaac cggcacggca gcgagcggga
     2581 tgatcgaggc acgccaggtc tgcaggaaca cgatcaccac cagcacgacg aggacgatcg
     2641 cttccagcag tgtccagatc actgcctcga tcgacttctg cacgaagcgc gtcgggtcgt
     2701 acacgatgtc gtaatcgacg ccgtgcggga aatcggcttt gagccgctcc atggtctcgc
     2761 gcacttcgtt ggacaacgcg atggcgttgg agcccggcgc ctggaaaacg ccgatcgcca
     2821 cggccggctt gttgttgagc aggctgcgca aggcgtagtc gcccgcgccc agttcaatgc
     2881 gcgcgatatc gcgcaaacgc gtgatgcgac cgtctggcga ggtcgcgacg atgatattgc
     2941 cgaactcctc ttccgtcgta agacggccgc gagcattgat ggaaagctgg aactcggtgc
     3001 cgtcggcgac gggcggcgcg ccgaccacgc cggccgcgac ctgcagattc tgttcgcgga
     3061 tcgcgttggc cacatcgctc gcggtgagat tgcgtgcggc gaccttctgc ggatcgagcc
     3121 acacgcgcat ggcgtaatca ccgccgccga acaccagcgt ctgacccatg ccgggaatac
     3181 gctgcaacac gtccttgacg ttcagcacgg catagttgcg caggtagagt tcgtcgtagc
     3241 gctcgtccgg cgacagcagg tgcaccacca tggtcagatt gggtgaactc ttttccgtgg
     3301 tgatgccgat ctgccgcact tcctcgggca tgcgcggcag cgcccgctgc acgcggttct
     3361 gcacctgcgt ctccgccaga tccacatccg tgccgatctt aaaggtcacg gtcaaggtca
     3421 acacgccgtc ggtcgtcgcc tgcgaggaca tgtagagcat gttctcgacg ccgttgatct
     3481 gttcttccaa cggcgcggcg accgtcgtgg cgatgaccgc cggattggcg ccgggataga
     3541 ccgcccggac tacgaccgaa ggcggcacga catccggata ttcgctcacc ggcagcttga
     3601 acagcgccag cacgcccagg atgaaaatga aggtcgacag cacgccggcg aagatcggcc
     3661 ggtcgatgaa caccttcgat agattgggac tcataaatcg tcctagtcct atgcaggttt
     3721 gccggcaccg cggtgagcgg ccggcaaggg atcgcccgcg cgctgcggca tgacgggact
     3781 gccgggacgc acgcgttgca agccgttcac cacgatcagt tcgcccggtt gcaagccttc
     3841 ctcgacgacg cgctcgttcg acatcaggcg cccgagcttc acttcgcgat acatcgcctt
     3901 gttgtcttcg ccgacgacga gcacgaactt gcgatcctgg tcggtcccaa tcgcgcgatc
     3961 gggcactagc acggcgttgc gttgcaccgc ttcggacagg cgcacgcgtg cgtagagacc
     4021 gggcgtcagc gcaccgtcct ggttatcgag caccgcgcgc atccgcaccg tgccggtggc
     4081 cggatcgacg tggttgtcga cgaacaccag ttgtccttcg cgcggaaaac cctcgtcgct
     4141 cgccagaccg atcgcgacgg gcatgctctt gcccggcgca ttgaggccgt aggcgagata
     4201 aacacgctcg ggcacttcga actcggcata gatcggcgtg gtggcgacga tggtggtgag
     4261 caacggcgaa ttgggcgcgt caccctggat gaggttgccc gcggtgattt cgatgcgtcc
     4321 cacacggcca tcgatgggcg ccgtcacgcg cgtgtaggcc agatcgagtc gcaggcgatt
     4381 ggcctgcgct tcggccgccc gttgcgcagc gatcgcatcc tgcacggccg ctgcgcgctg
     4441 atcgagttcc tggcgcgatg tcgcattcac cttcaccaac tcttcgctgc gcgccagttc
     4501 cttgcgcgcc aattcgaggc gggtcttggc cgccgcgacc tctgcttcgg cgcgtgcgag
     4561 gttggcctgg aagggccgcg gatcgatcac caccagcacg tcgcccttct tcaccatcgc
     4621 gccctgcttg aagcgcactt cctcgatgta acccgtcaca cgtgcgcgca cttccacgga
     4681 ctccaccgcg gcaagacggc cggtgaagat gtccgattgg acgatgttgc gcgcttcggc
     4741 gggcgccacg gtgacgggcg gtgccggcgg cgcgccggta tcggcttgcg acgcggcatc
     4801 gcggcagcct tgcaacgcca gcacgagcgc aacgacggca atcaggctgt aggaggaagc
     4861 gtaagtaggc atgtcggact cgtcgtgttc taggtgtgaa ggggcgtgcc gcgcgcaggc
     4921 gccagcaaag gttcgaggaa gtcgccgatt tcgcgccagg cggcggcgga ccagccggcc
     4981 ttgtcgagtc gcgcaacgcg ggtatgcaca ccggccgcgt cgagcttggc gccgtagtgc
     5041 tgcgcctcgt ctcgaagcgg atcgtcgtcg gcagtcagga tcagcgcggg cggcagttgc
     5101 gccagacgcg tgcacagacc cggcgccgca tagggatgca gacgatccgc ggtgttgggc
     5161 aggtaacggt gatacgcctc gccgcaggcg cgcgacgcct cctctgcctg acgcatcgag
     5221 caggaggtga gactggggtc gagcatcggc gacaacagca cttgcgcaac gacgtgcggc
     5281 ccgccgcgat cgcgcgccat catcgcggcg acggcggcga gattgccgcc ggcctcgtgg
     5341 ccgatgacgg cgatgcgctt ggcgctccac aagcctgcac cggcgttgtc atgcacccat
     5401 cgcagtgcgg tataagcatc ttccgccgca gcgggaaagg gacactcgac cgccagactg
     5461 tacgcaggcg tgatgaccgc aacgccgtaa cgctcggcca acgcgcgcgc cgccgcgtcg
     5521 gcttcttcca ggccgccgct gacgaaacgc ccgggatgga aatagaccag cgccggcggc
     5581 gtggaacggc cgctgccgcg tcgatgcacg cgcatgcgca aggtgcgatc gaagccggtg
     5641 caactgcggt cttgcgcggc gatatctgcg gcgggtacaa agggcaccat gggaggggct
     5701 taaaggcgaa gaagaaggcc gcgagcatat tcgcctgccg ggcgcagaca aagcggctaa
     5761 gatggaaagc actgttcagc cttcaccaac aatgaggcca acatggaccg cttgcaggca
     5821 atggaagctt ttgtgcatgt cgtgcagaca ggcagtttca cgcgcgctgc cgaccggctg
     5881 cgcttgccga aagcgactgt caccacgtgg atacagaatc tggaagcgcg tttgcgcgtg
     5941 aagttgctca atcgcaccac gcgcaaggtg agcgtcacgg cagacggcgc ggcttattac
     6001 gagcgatgcc tgcgcatcct caacgaagtc gaggaaaccg aaagcgtgct gacgcgcacc
     6061 gcaacctcac cgcaagggcg gctgcgcatc gacgtgcccg tctccttcgg acgctacatc
     6121 ctcattcccg cactgcccga cttctgcgcg cgctatccgg acatccagct ggaaatcggc
     6181 tgcaccgacc gtccggtgga cctgatcgag gaaggcgtcg attgcgtcgt gcgcggcggc
     6241 gagttgtccg atgtctcgct ggtggcgcgg cgtatcgcca ccctgcactt cgtcacctgc
     6301 gcgcatcgcg actacatcat gcgccacggc acgccgcgtc atcccaacga tctggtcgat
     6361 caccgctgca tcggttactt ctccggcaag acgggcaagc cgtgcaactg ggatttcgcc
     6421 cgcgacggcg aacgcatcga gttcgacgtg cagccctatg tgtcggtcaa cgacagcgac
     6481 gcctatcttt ccgcggggct cgcaggtctg ggcatcatgc aggtgtcgat gatcaaggtg
     6541 cgtcacgcgc tcgacaaggg tctgctggtg gaggtgctgc ccgagtggaa agtcgatccg
     6601 ctcccggtgt atgtgatgta cccgcccaac cgtcacctgt ccgccaaggt gcgcgtgttc
     6661 gtcgactggg tcgccgccct ggtcgaaggg cgagacgaaa tgccgctgct ggacgagcag
     6721 ctctgtctgc tgcggcagcc gcagaacctc gccgcctgag acacatcgtg tacgttcttc
     6781 gcgcctttgc gcgctgccgc acgtgcgtta cgacgctctg atcgtcggcg gcggtcacaa
     6841 cggcctcacc tgcgcctgtt atctcgccgc ggccggtttg cgcgtgcgcg tgctggagcg
     6901 gcgccacgtc gtgggtggtg cagccgtgac cgaggaattc catccgggtt tccgcaattc
     6961 ggttgccagc tacaccgtca gtctgctgaa tcccaaggtc atccgcgatc tgcggctggc
     7021 cgaacacggt ttgcgcatcg tcgaacggcc ctactccaat tttctgccgc tggacgacgg
     7081 cgatgcgctg gaagtcggcg gcggcctggc tgcaacgcaa cgcgaagtgg cgcgcttctc
     7141 cgcacgcgat gcggagcgtc tgccggcgta ttacgacacc atcgaacgcg tcgccgacgt
     7201 gctgcgcgat ctggtgttgc agacgccgcc caatgtcggc ggcggcctgc acgatctgct
     7261 cggtgcactg cgtctgggca tgcgcatgcg cggcctcgac atggcggcga ggcgcgatct
     7321 cctcgacctg ttcaccaaga gcgcgggcga gatgctcgac tggtggttcg agtccgagcc
     7381 catcaaggcg gcgttcggct tcgacgcggt ggtcggcaac ttcgccagtc cttacacacc
     7441 gggctccgcc tacgtgctgc tgcaccactg cttcggcgaa gtgaacggca aacgcggcat
     7501 ctggggccat gcgatcggcg gcatgggtgc cattacgcag gccatggcgg ccgaagcgca
     7561 agcacgcggc gtggagatca gcactgcgag cgcagtgtcg caagtgttga cacgggatgg
     7621 ccgcgccacc ggcgtggtgc tggaggacgg tacggaactc gaagcgcgtt tgatcgtctc
     7681 caatctcaat cccaagctgc tgtttctcga tctgatcgca ccggacgcgc tggaggagga
     7741 tttccgcacg cgcatcgagc gctggaaatg tgcttcgggc acgttccgca tgaacgtcgc
     7801 actgtcggaa ctgcccgact tcagcgcgcg acccggcacg caggtgcaac cgcatcaccg
     7861 ctccggcatc gtcatggcgc cctcgctggc ctatatggat cgcgcccacc aggaggcgcg
     7921 cgagcacggc tggtcgcgcg cgcccattgt cgaaatgctc atccccagca cggtggacga
     7981 cacgctggcg ccggaaggca tgcacgttgc cagcctgttc tgccagcact tcaacccggt
     8041 gctgcccgac ggccgcagtt gggacaacgc acgtgaaacc gcggccgatt tgatcatcga
     8101 taccgtgagc cgctacgcac ccaatttcaa gtccgccgtc attgcgcggc agattctgtc
     8161 gcccctggac ctggagcgca ccttcggcct agtgggcggc gatatcttcc acggcgcgct
     8221 gtcgctcgac cagctttatt ccgccagacc ggtgctcgga cacgcgaatt accgcgcacc
     8281 gatcaaggga ctctaccttt gcggctcggg cacccacccg ggcggcggcg taacgggcgc
     8341 gcccgggcat aatgcggcgt ttgaaatttt aagggactgg cgcgcgcaca agctttagcg
     8401 cagcggaatt cgggagggaa atactggatg cgtagcactt ctgcagaaac aacaacacgc
     8461 ggctcaccgc gtccggaact gtccatgttc gatgccacgg cgctgatcgt cggcctggtc
     8521 atcggcgccg ggattttccg cctgccttcg ctcgtcgcag cgaataccgc caacgaatgg
     8581 gtgttctacg gcgtctggat cgccggcggt ctgatttctc tcatcggcgc gctctgctac
     8641 gcggaactgg ccaccaccta tcccaacgcc ggcggcgact actattttct gcaacgcgct
     8701 ttcggtcgcg gcttgtcgtt cctgttcgcc tgggcgcgca tcgcggtcat taccaccggc
     8761 tccattgccg tgctgggtta taccttcggc gattacgcgt ccaatctcta cagcctcggt
     8821 tcgaattcgt cggcgatcta cgccgcattg gcggtgttgg cgttcacggc gatcaacgtc
     8881 agcggcatac gcgagacacg cggcacgcag aatgtgctga cgacgttcga ggtcttcggt
     8941 ctgctcgcag tcgtcgtgac gggcttgctg ctcatcgcac ccgaacaagc ggcgcccgcg
     9001 gcagcggcga cggaacccaa agcgtggacc gcgggaattc cgctggccat tctgttcgtg
     9061 ctgttcacct acggcggctg gaacgaaggc gcctacattt ccgcggaatt gaaaaaccgg
     9121 cgcgacatgg tgcgtgcgct ggtggtgagc cttgccatcg tcacgctgct ctatctgctg
     9181 gtgaacatcg cctacgagcg cacattggga ttggccggca tggcggcgag cgagaccatc
     9241 gcctcggacg tgctcggccg ctacttcggc gactggggcg cacacatcat cagcgcgatc
     9301 gtcgccgtgt cggcgctcac gtccatcaac gcgacgatcg tcgtgggcgc gcgctccaac
     9361 tatgcgctgg ggcgcgactg gccgatcttc ggttggctcg gctactggga cgacaaggcc
     9421 gatgcgccgc gcaacgcgct ctacgtgcaa tgcgccgtcg ccgtgctgct catcgttgtc
     9481 ggcgccttcg gcggcaagat cgaaaccatg gtggactaca ccatgccggt gttctggttc
     9541 ttcttcctca tgacgggaat cggcctgttc gtgctgcgtc acaaggaccc gcacacgccg
     9601 cgaccgttca aggtgccgct ctatccgctg acgccgctga tcttcgtggc gacttgcgcc
     9661 tatctgttct attccagcgt cgcctacgtc aagggcggtg cgcaactggg catcgccgta
     9721 ctgctgctgg gcggcgtgct gctgctgttg aacacacgcc gcgccaaggc gcagcccgca
     9781 caataagttc gaaaccgaag gaactgtcat ggaacgtcgt cgttttatca agctgctctc
     9841 cggcgctgcc gcccttggcc ttgccggccg cgcggcctac gcgcaaccga tcccgccggg
     9901 tccgggccat catctggacg cgcccttcgt ccccactcgc gaggaagtgg tcgaggagat
     9961 gctgcgcgtc gccggcaccg gaccggagga catcgtgtac gacctcggtt gcggcgacgg
    10021 ccgcattccc atcatcgcgg cgcagaagtt cggcgcacgc ggcgtgggga tcgacatcga
    10081 ccccgaacgc atccacgaat cgcgcgaaaa tgcccgtgag gccaaggtga cggatcgcgt
    10141 caagttcatc gccgaggatc ttttcctcgc cgatatcagc gaagccaccg tggtgacgct
    10201 ttatctgctg ccctcggtga atgtgcgttt gaaaccaaag ctgcaaaagg aactcaaacc
    10261 cggtacgcgt gtggtgtcgc acgatttcgg catgggcgag gactggccgc ccgagcgcac
    10321 ggtgaaactg ggcggcgaca tcatccatct gtggcgcatc gagtagcagc gggcctgggg
    10381 cccggccgag tgcgatccgc tcgctcgtac acgcgccgca cttgccggtc ccttctctat
    10441 ccgaccttct gctgaaggtt ttttacgccc cgggtgatgc cgggcgcaaa gcgctgccgt
    10501 tgtttgtaat gggcggcacc cagtcggagc acatgcatgc cagatgagga tttttcgctt
    10561 tattgggacg aagacgacga cttcgggctg gacaacgagc cggtgttcga cgagcacgtc
    10621 tatccggagt acctcttcga cgttccctac gtccccactt ccccggaact ggtggacgcg
    10681 atgctgcgtt tcgccggcac cggcccgggc gatgtggtgt atgacctggg ctgcggcgac
    10741 gggcgtatcg ccatcgccgc agcggaaaag ttcggcgccc gcgccgtcgg catcgagatc
    10801 gacgagcatt tgatcgccga agcgcgcagc aacgccgcgc tggccgacac ggaaggccgg
    10861 gtgcgtttcg tgcacgccga cttccacgag gcgcacatcg ccgacgcaac ggtcgtcgca
    10921 atttacctgc tgcccgaggt caaccttctg ctgaagccgc gattcctgca ggaactgcgg
    10981 cccggcacgc gcatcgtgtc gaactacttc ggcatgggcg aggagtggcc tgccgaccgc
    11041 acgactcgcg tcgggcagga tctcatacaa ctgtggcgcg tcccctgaac ggggccggcg
    11101 cacacgatac gcgacacagg actcaacacc cgcacaggat caggaatgaa actcggattg
    11161 atcggattgg ggcgcatggg cgcgaacatg gcacgccggc tggcgcgcac cggcatcgaa
    11221 gtggtcggct acgacgtgag caccgaggcg cgcgccgcgc tgggcagcga gaccggcgta
    11281 tcgctggtgg acagcatcga tgccgtggtc gcgaacctgc ctgcgccgcg cgcgctgtgg
    11341 atcatgctgc ccgccggggc gattacgcag gaagccatcg accggctcgc cacgctggcc
    11401 gcggccggcg atctgatcgt ggacgggggc aacgcctact acaaggattc gatgcgtcgc
    11461 gccgaggcgc tggtcgagcg cggcatcgac ttcatcgacg cgggcgtctc gggcggtgtg
    11521 tggggactgg agaacggcta caccctgacg ctcggcggcg aggcgcatgc ggtcaagcgg
    11581 ctggagccgg tggcgcgcgc gctcgcgccc gcgcccgatc gcggctggct gcactgcggt
    11641 ccggccggca gcggccactt cgtcaagatg gtgcacaacg gcatcgagta cggcgccatg
    11701 caggcctatg ccgaaggctt cgcgctgctg gccgccaagt cggagttcga gctcgacgtg
    11761 ccgcagatcg cggagacctg gcgccatggc agtgtcatcc gttcgtggct gctcgatctc
    11821 accgccgaag cactggcgca gaagccggac ctggacggca tcgcgcccgt ggtcggcgat
    11881 tcgggcgaag gccgctggac cgcgctggag ggcgtcgaac tgggcattcc cacgccggtg
    11941 acgagtctgg cgctgatgat gcgtttcgcc acccagggca ggaacgacta tcccgccaaa
    12001 ctgctggcgc tgatgcgcaa tgccttcggc ggtcacgcca ttacggccaa gcgctgacag
    12061 gacggaacgc atggtggtta tcgtcatggg cgtgtcgggt gcgggcaaaa ccacggtcgg
    12121 caagctgctg gcgcatcgcc tcggcgcgca ttttctcgac gcggacgatt tccatcccga
    12181 ggaaaacgtg gaaaagatgc gcggcggctc gcccctggcc gagagcgatc gcgcgccctg
    12241 gcttgcgcaa ctcaacaagg aattgcgcaa gcaccatttg cgcggcgagc gcgtcgtgct
    12301 cgcctgttcg gcgctccggc gcagccatcg tgagcagttg ctcgagggat tgccggcggc
    12361 gcaagtggtc tatctgcggg gcagccgggc gctgctcgcg tcgcgcctgg cgcagcgcaa
    12421 ggggcatttc atgccgcccg cactgctcga tacccagttc gaagcgctgg aagagccgcg
    12481 caacgccgtg accgtggatg tgagcggcac gccggaaggc gtggtcgagg cgatctgcgc
    12541 ggcgctggat ttaaaggctt gaaccacaga ggcacagaga aaaatcttga accaccaagg
    12601 cgccaagaca ccaagttatt gatcgttatg cttggtgctt tcttggtgcc ttggtgtctt
    12661 ggtggtgagg tttttccaag ctttctcaat ccgcccagcc cgggtcctcg cgatcgagga
    12721 tacgcttgca ggcggcgatg aaggcctcgg cctgttcgcg gtcctcgacc atcagtttca
    12781 ccgtctccga cgcgaccttg tgcgagatcc gcttgagcgg cttgcccgtg ctttgcgcga
    12841 tgacctgcag catgcaggcg cgc
//
LOCUS       DATGOU010000022         7005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_53326, whole genome shotgun sequence.
ACCESSION   DATGOU010000022 DATGOU010000000
VERSION     DATGOU010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7005
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53326"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="VKZ48_01335"
     CDS             complement(<1..1241)
                     /locus_tag="VKZ48_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLU76527.1"
                     /translation="MKKMKLVMVGNGMAGVRTLEELLKLAPDLYDITVFGAEPYPNYN
                     RILLSPVLAGEMSVQEIILNDLDWYRDNGIKLHLNKEAVQIERRARKVIAADGTEENY
                     DRLLLATGSNPFMLPVPGKDLEGVLSYRDIQDTDAMIEASRHHKHAVVIGGGLLGLEA
                     ANGLKLRGMNVTVVHLMDWLMEKQLDRTAAQMLQCALEHKGLHFLLGKQTREIVGGED
                     GRVKGVRFEDGSEIATDLVVMAVGIRPNTALAESAGLHCDRGIVVNDTMQTYDPKIYA
                     VGECVAHRGTSYGLVAPLFDMAKVCANHLAFYGIGRYTGSVTSTKLKVTGIDLFSAGD
                     FTGGPDTEEIVLHDAVGGVYKKLVVKDNRIVGGVLYGDTSDGAWYYQLLRDQQSIAEI
                     RDHLLFGQDNLGNTGHQGQNKA"
     gene            complement(1417..2397)
                     /gene="ybiB"
                     /locus_tag="VKZ48_01340"
     CDS             complement(1417..2397)
                     /gene="ybiB"
                     /locus_tag="VKZ48_01340"
                     /inference="COORDINATES: protein motif:HMM:NF006005.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HLU76528.1"
                     /translation="MNYAQIIREIGRGAHGAHELGIEEAQRLFAAMLDGGVPELELGG
                     ILLAMRMKTESVNELTGFGQALSERVHRLLPPTDAVRPVVLPSYNGARRQPNLVPLVA
                     LALQRIGVPVLVHGSLEGNGRIATAYILRELGIMPCATLAQAQRSLNEGRVTFVPTAV
                     LSPGIAQLLGMRARLGVRNSAHTIVKLMDPFDGNALRVVALTHPDYLLKVGEVLQRTR
                     GAALLMKGTEGEPYAAPRRRPSMVLYQGGRHEVLFEQERMPDEAPSFAPQDLEAAATA
                     RYVHSAIDGALPIPLPVVNQIACCLYGCGYADDLNQAKAIAAVQTRSMAA"
     gene            complement(2417..5152)
                     /locus_tag="VKZ48_01345"
     CDS             complement(2417..5152)
                     /locus_tag="VKZ48_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HLU76529.1"
                     /translation="MKRETKSTCCYCGVGCGLIIESEGARIVGVRGDPEHPANFGRLC
                     TKGSTLHLTARHDTRALYPEMRRERTSPRRRAGWDEALDHVAARFAETIRTHGPDSVA
                     FYISGQLLTEDYYVFNKLAKGLIGTNNIDTNSRLCMSSAVAGYKATLGADAPPACYED
                     IAHAQCIFIAGSNTAIAHPIVFRRIEDERAKRPDMKVIVVDPRRTDTASTADLHLPIL
                     PGTDVALYNAMLHVLLWEGACDTAYIRAHTEGFDALKETLRRYTPEFAARICGVPAAD
                     IVQAAQWFGQSPATLSLYCQGLNQSTHGTDNNAALINLHLATAQIGKPGAGPFSLTGQ
                     PNAMGGREVGGLSNLLSAHRDMTNAEHRAEVARLWGVDEVPAVPGKSAVEMFDALRRG
                     EIKAIWIACTNPAQSMPDLQRVHEALDAATLVVVQEAYANTDTAAFADVLLPATTWGE
                     KTGTVTNSERRISRVRAAVPPPGEARHDWQVVVDFALRLGRALGDARARRLFPYAEAE
                     AIFNEHRESTRGRDLDITGLSYAMLDTRGPQQWPLPEGAAAGKARLYEDGLYPTPNGR
                     ARFVVAEHRPTAEDADARYPLHLTTGRMRDQWHGMSRTGTVARLYNHVGEPLLSMNPN
                     DMRRRGLREGDIVRMKSRRGAQIVRVTAADDVRPGQAFMPMHWGGQFMRGLGVNALMA
                     PDFDPVSKQPELKHAAVQVEKLELPETLVAMRRFAAGADPAAVALMQALQPLLARFDY
                     ATLALAGRDNEVVVLRGYACEPVSVELLDVLDALLGLDTAEQTMRYIDPRRGVYKAAR
                     VEGDTVTAIRLCGETAAQEWVRNMMIAGASAEAVRPWILAPVSSPPRGTLARGRIVCT
                     CMDVSASEIRAQVAAGANLAALQTTLKCGTQCGSCVPEVRRMCEVRPLLGEARAA"
     gene            complement(5163..5954)
                     /locus_tag="VKZ48_01350"
     CDS             complement(5163..5954)
                     /locus_tag="VKZ48_01350"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLU76530.1"
                     /translation="MSRSLQIEGVEMRFDTKSGPFIALHDIELHIREGEFISLIGHSG
                     CGKSTLLNLIAGLLRPTRGAMILANKEIAGPGPDRGVVFQNHSLLPWLTCYENIHLAV
                     ERVFGATEGKAKLKERAQAALELVQLAHAAHKYPHEISGGMKQRVGIARALSIEPKVL
                     LLDEPFGALDALTRAHLQDELMNIVAKTRSTVVMVTHDVDEAVLLSDRVVMLTNGPAA
                     TIGEVLAVKLDRPRDRLTLATDPEYLRCREAMLQFLYRKQMRQAA"
     gene            complement(5965..6708)
                     /gene="ntrB"
                     /locus_tag="VKZ48_01355"
     CDS             complement(5965..6708)
                     /gene="ntrB"
                     /locus_tag="VKZ48_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HLU76531.1"
                     /translation="MALGLASFVAVWAGIAHFGGQLPTPAATLDAALALFADPFYNKG
                     PNDQGIGWNVLNSLGRVGIGFGAAALIGIPLGFLIGRFGFLSDMAAPIIGLLKPVSPL
                     AWLPIGLLVFQAANPAAIYVIFICSVWPLMINTAVGVRQVPQDYLNVARVLNLSEWTV
                     FRKILFPAALPHVITGVRLSIGVAWLVIVAAEMLTGGVGIGFWVWDEWNNLNVQHIIV
                     AIFVVGIVGLVLEQSLIALARRFSYDLKS"
BASE COUNT         1244 a         2306 c         2186 g         1269 t
ORIGIN      
        1 gctttgttct ggccctggtg gccggtgttg ccgaggttgt cctgaccgaa aagcaggtga
       61 tcgcgaattt cggcaatgct ctgctgatcg cgcaacaact ggtaatacca ggcgccgtcg
      121 ctggtgtcgc cgtacagcac gccgccgacg atcctgttgt ccttgaccac cagcttcttg
      181 tacacgccgc cgacggcgtc gtggagcacg atctcttcgg tatccggacc cccggtgaaa
      241 tcgccggcgg agaaaagatc gatgccggtg actttcagct tggtcgacgt gaccgaaccg
      301 gtgtagcggc cgataccgta gaacgccaga tggttcgcgc aaaccttcgc catatcgaac
      361 agcggcgcga ccaacccgta agaggtgccg cgatgcgcga cgcattcgcc tacggcataa
      421 atttttggat cgtaggtctg catggtgtcg ttcaccacga tgccgcgatc gcaatgcagc
      481 cctgccgatt cggcgagcgc tgtgttcggg cgaatgccga ccgccatcac gacgagatcg
      541 gtcgcgatct cgctgccgtc ttcgaagcgc acgcctttga cgcgcccgtc ttcgccgcca
      601 acgatttcac gggtctgctt gccgagcagg aaatgcagtc ccttgtgttc caacgcgcac
      661 tgcagcatct gcgcggcggt gcgatcgagc tgcttttcca tcagccagtc catcaggtgc
      721 accaccgtaa cgttcatgcc gcgcagtttg agaccgttcg ccgcctccag gcccagcaat
      781 ccgccgccga tcacgacggc gtgcttgtgg tgccgggagg cctcgatcat cgcgtccgta
      841 tcctggatgt cgcgatagct cagcacgcct tccagatcct tgcccggcac cggcaacatg
      901 aagggattcg aaccggtcgc gagcaacagg cgatcgtagt tttcttccgt accgtccgcg
      961 gcgatcacct tgcgtgcgcg gcgctcgatc tgcaccgcct ccttattgag atgcagcttg
     1021 atgccgttgt cgcgatacca gtcgaggtcg ttgaggatga tctcctgcac gctcatttcg
     1081 cctgcgagca ccggcgacag cagaatgcgg ttgtagttgg gatacggctc cgcaccgaac
     1141 acggtgatgt cgtaaagatc gggcgcgagc ttgagcaatt cctcgagcgt gcgtacgccc
     1201 gccatcccgt tgcccaccat caccagcttc attttcttca ttgacgcact ccgacacctg
     1261 cattggcacg cacgtttcag caacacctat gccatgcgac aagcgcttgc aaatcaatcc
     1321 gctattgagc aggcgcgcag cgtcaatgcc ggttcttagt gcacgagacc gatgcggtgc
     1381 cctgcaatgg ggcagcagcg atgccgcgct gcaccgtcac gcggccatgc tgcgcgtctg
     1441 cacggcggcg atggccttgg cctgattgag atcgtcggca tagccgcagc cgtaaaggca
     1501 acaagcgatc tggtttacca ccggcagggg tatgggcaac gcaccatcga ttgcgctgtg
     1561 cacgtagcgc gcggtggccg cggcttcgag atcctgcggc gcgaagctgg gcgcctcgtc
     1621 gggcatgcgt tcctgctcga acagcacctc atgtctgcca ccctggtaaa gcaccatgct
     1681 gggacggcgg cgcggcgcag catagggctc gccttccgtc cctttcatga gcagcgccgc
     1741 accgcgcgtg cgctgcagca cctcgcccac cttcagcagg taatccggat gcgtcagcgc
     1801 aacgacgcgc aaggcattgc cgtcgaaggg atccatcaac ttcacgatgg tgtgcgcgct
     1861 gttgcgcacg cccaaacgcg cacgcatgcc gagcagttgt gcgatgcccg gagacagcac
     1921 cgccgtcggt acgaaggtga cgcgaccttc attcaaggag cgttgggcct gcgccagcgt
     1981 cgcgcagggc atgatgccca gctcgcgcag gatgtaagcc gtggcgatcc ggccgttgcc
     2041 ctccagcgat ccgtgcacga gcacgggcac gccgatgcgc tgcagtgcca gcgcgacgag
     2101 cggcacgaga ttgggctgac ggcgggcgcc gttgtacgag ggcagcacga ccggccgcac
     2161 ggcatcggta ggcggcagca gacgatgaac ccgttcgctc aacgcctgcc cgaaaccggt
     2221 caactcgttc accgattccg ttttcatgcg catggcgagc aggatgccgc ccagttccag
     2281 ttccggcacg ccgccgtcca gcatcgcggc gaaaagccgc tgcgcttctt cgatgcccag
     2341 ttcgtgcgcg ccatgtgcac cgcgaccgat ttcccgaatg atttgtgcgt aattcatttg
     2401 ctgctcgcag aactgttcat gctgcgcgcg cctcgcccaa caatggccgc acctcgcaca
     2461 tccgccgcac ttccggaacg caggaaccgc attgcgtacc gcatttaagc gtcgtctgca
     2521 gcgccgcgag gttggcgcct gccgcgactt gcgcgcggat ctccgatgcc gatacatcca
     2581 tgcaggtgca cacaatgcgc ccacgcgcga gcgtgccgcg cggcggcgac gagaccggcg
     2641 cgagtatcca cggtcgcacc gcctccgccg acgcgccggc gatcatcatg ttgcgcaccc
     2701 attcctgcgc ggccgtctcg ccgcacaggc gaatggcggt cacggtatcg ccttcgacgc
     2761 gtgcagcttt gtaaacgccg cgacgcggat cgatgtagcg catcgtctgc tcggccgtat
     2821 cgagaccgag caaggcatcg agcacatcga gcagttcgac ggacaccggt tcgcatgcgt
     2881 aaccgcgtag cactaccact tcgttgtcac gaccggccag cgccagggtg gcgtagtcga
     2941 agcgcgcgag taaaggctgc agtgcctgca tcaatgcgac tgcggccgga tcggcgccgg
     3001 cggcgaagcg tcgcatcgcg accagcgtct ccggcagttc gagtttctcc acctggacgg
     3061 cggcgtgttt gagttcgggc tgcttcgaca ccggatcgaa atccggcgcc atgagggcgt
     3121 tcaccccgag cccgcgcatg aactgcccgc cccagtgcat gggcatgaaa gcctgtccgg
     3181 gtcgaacgtc gtccgcggcc gtcacgcgca cgatctgcgc cccgcgccgg cttttcatcc
     3241 gcacgatatc gccttcgcgc aacccgcgac gccgcatatc gttcggattc atggacaaca
     3301 gcggttctcc gacatggttg taaagccgcg ccaccgtacc ggtgcggctc atgccgtgcc
     3361 attgatcgcg catgcggccg gtcgtgagat gcagcggata acgcgcatcg gcatcttccg
     3421 ccgtgggtcg gtgttcggcc acgacgaagc gggcgcgacc gttcggtgtg gggtacaggc
     3481 catcttcgta gagtcgcgct ttgccggcgg ccgcgccttc cggcagcggc cactgctgcg
     3541 gcccccgcgt atccagcatc gcgtagctca gtccggtgat gtcgaggtcg cggccgcgcg
     3601 tgctttcgcg atgctcgttg aaaatcgctt ccgcttccgc atagggaaag agtcgccgtg
     3661 cacgcgcatc gcccagcgca cgacccagcc gcaacgcgaa gtcgacgacg acctgccagt
     3721 catgtcgtgc ttcgccgggc ggcggcacgg cggcgcggac gcggctgatg cgccgctccg
     3781 agttcgtcac cgtgccggtc ttctcgcccc atgtcgtcgc gggcagcaac acatcggcga
     3841 acgcggcggt atccgtgttg gcataggctt cctgcacgac caccaaagtt gccgcgtcca
     3901 aagcttcatg cacgcgctgc aaatcgggca tcgactgcgc gggattggta cacgcgatcc
     3961 agatggcctt gatttcgccg cgccgtagcg cgtcgaacat ttccacggcc gacttgccgg
     4021 gcacggcggg cacctcgtcc acgccccaga ggcgggccac ttcggcacga tgttcggcgt
     4081 tcgtcatgtc gcggtgtgcg gacagaagat tcgacaaccc gccgacctcc cgaccgccca
     4141 tggcattcgg ctgaccggtg agcgagaacg gaccggcgcc gggcttgccg atctgcgcgg
     4201 tagccaggtg cagattgatc agcgcggcat tgttgtccgt gccgtgcgtc gattggttga
     4261 ggccctggca atacaaggag agtgtggcgg gcgattggcc gaaccattgc gccgcctgca
     4321 cgatgtccgc cgccggcacg ccgcaaatgc gcgccgcgaa ctcgggcgta tatcgccgca
     4381 aggtttcctt cagcgcatcg aacccttcgg tgtgcgcacg gatgtacgcc gtgtcgcacg
     4441 ccccctccca caacagcacg tgcagcatgg cgttatagag cgcgacgtcc gtgccgggca
     4501 gaatgggcag atgcaggtcg gcggtcgatg cggtatcggt gcgacgcgga tcgacgacga
     4561 tgactttcat atcgggtcgt ttcgcccgtt cgtcttcgat gcgacggaac acgatgggat
     4621 gcgcgatggc ggtgttggag cccgcgatga aaatgcactg cgcatgcgca atgtcctcgt
     4681 agcacgcggg cggcgcatcg gcgccgagcg tcgccttgta gcccgccacg gcggaggaca
     4741 tgcaaagccg cgagttggta tcgatgttgt tggtgccgat caaccccttc gccagcttgt
     4801 tgaagacgta ataatcctcg gtcagcagct gcccggagat ataaaaggcc acgctgtccg
     4861 ggccgtgggt gcgaatggtc tcggcgaagc gcgccgcaac atgatcgagc gcttcgtccc
     4921 aacccgcgcg gcggcgcggc gaggtgcgct cgcgacgcat ttccggatac agcgcgcgcg
     4981 tgtcgtgccg tgcggtgagg tgcagcgtcg aacccttggt gcagaggcgg ccgaagttcg
     5041 ccgggtgctc gggatcgcca cggaccccga cgatgcgcgc gccctcgctt tcgataatga
     5101 gaccgcagcc gacgccgcag tagcagcagg tcgacttcgt ctcgcgcttc attgccgcat
     5161 cctcaggccg cctgtcgcat ctgtttgcga taaaggaatt gcagcatggc ttcgcggcag
     5221 cgcaggtact cgggatcggt ggcgagcgtc agccggtcgc gcggacgatc gagcttcacc
     5281 gccagcacct cgccgatggt cgccgccggc ccgttggtca gcatcaccac gcgatcggac
     5341 aggagtacgg cttcgtcgac gtcgtgcgtc accatcacca ccgtgctgcg cgtcttggcg
     5401 acgatgttca tcaactcgtc ctgcaagtgc gcgcgggtca gtgcgtccag cgcaccgaag
     5461 ggctcgtcga ggagcagcac cttgggttcg atggacagcg cccgcgcgat gcccacgcgc
     5521 tgcttcatgc cgccggaaat ttcgtgcgga tacttgtgcg ccgcatgcgc cagttgcacc
     5581 agttccagcg ccgcttgcgc ccgctccttg agtttcgctt taccttccgt cgcgccgaat
     5641 acgcgctcca cggcgagatg aatattctcg tagcacgtca accagggcaa cagcgagtgg
     5701 ttctggaaca ccacgccacg atccgggccg gggcctgcga tctccttgtt cgccaggatc
     5761 attgcgccgc gcgtcggccg caacagaccg gcaatgagat tgagcagcgt ggatttgccg
     5821 caccccgagt gaccgatcag cgagatgaac tcgccttcgc ggatatgcag ttcgatatcg
     5881 tgcaaggcga tgaagggtcc gcttttggtg tcgaagcgca tttccacacc ttcgatttgc
     5941 agtgatctgc tcatgatgcg ttcctcagga cttcaggtcg tagctgaagc ggcgcgccag
     6001 cgcgatcagc gactgttcca ggacgaggcc gacgatgccg accacgaaga tggcgacgat
     6061 gatgtgctgc acgttgaggt tgttccactc gtcccacacc cagaagccga tgccgacgcc
     6121 gccggtgagc atctccgcgg cgacgatgac gagccacgcc acaccgatgg acagacgcac
     6181 gccggtgatc acatgcggca acgcggcggg gaagagaatc ttgcggaaca ccgtccactc
     6241 cgacagattg agtacgcgtg ccacattgag atagtcctgc ggcacctgtc gcacgccgac
     6301 cgccgtgttg atcatcagcg gccacacgct acagatgaag atcacgtaaa tcgccgcggg
     6361 attggccgcc tggaagacca gcagcccgat gggcagccag gccagcggac tcaccggttt
     6421 cagcagaccg atgatcggcg ccgccatatc cgacagaaag ccgaagcgtc cgatgaggaa
     6481 gccgagcgga ataccgatca gggccgcagc gccgaagccg atgcccacgc gtcccagcga
     6541 gttgagcacg ttccaaccga tgccctggtc gttcggccct ttgttgtaga agggatcggc
     6601 gaacagggcg agcgccgcat ccaacgtggc cgcgggcgtg ggaagctgac caccgaagtg
     6661 cgcgattccc gcccacaccg ccacgaagct cgcaaggccg agcgccatcg cgacggcacg
     6721 ttgcaaccac tccggtgcgc gcgtgcgcac ataaccgtgt cccggatcgg cgtcggacga
     6781 gtccgccgcc ggcacattac gccgccgtgt cgcctgcgcc accggcgggg tcgactccgg
     6841 ttcaggcaaa ggcaccgatt tgagatttgc cgcactcatg atgatgcctc ctaggccaca
     6901 cgaatcttga aggactgggc gtacttcttc gcgtccttgc cgtcccacac cacgccatcg
     6961 atcagcttcg ccgtacggaa cggatccttt ggcaccgaaa ctttc
//
LOCUS       DATGOU010000023        30181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_4944042, whole genome shotgun sequence.
ACCESSION   DATGOU010000023 DATGOU010000000
VERSION     DATGOU010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 30181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30181
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4944042"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..672)
                     /locus_tag="VKZ48_01360"
     CDS             complement(118..672)
                     /locus_tag="VKZ48_01360"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HLU76532.1"
                     /translation="MFRSTVHRSAGVFAASVLTFAVAAPVGAEVYKWTDAQGVVHYSN
                     VAPRDVRSAERVPENRLSIVTLPKPSAEEVRALNERLQNRRINQLEEALRAARRSSDV
                     AYAPAEGSGVYYPASGWIGGHAEPPLGEVIIDRDLDADGNSRRTNIRFIPRYVIKPGP
                     GGIGGQAVAPPAPPKRAREVHRLP"
     gene            937..2292
                     /gene="glmU"
                     /locus_tag="VKZ48_01365"
     CDS             937..2292
                     /gene="glmU"
                     /locus_tag="VKZ48_01365"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013236343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HLU76533.1"
                     /translation="MNIVILAAGQGKRMHSDRPKVLHELAGRPLLAHVVETARALGPD
                     RICVVYGHGGEAVREAIGEDGLQWALQNPQLGTGHAVQQALPLLGKQGPTLVLYGDVP
                     LISADSLRPLVEAARDGIGLLTVEMDDPTGYGRIVREGGAVRRIVEHKDATPEQRQLR
                     EVNTGIMAVPTDRLAAWLAALRNDNAQGEYYLTDIIAQAVAEGLPVRAFQPTAAAETL
                     GVNSKTQLAELERIYQRNRAAALLEQGVTLADPGRFDVRGTLRCGRDVYIDVNCVFEG
                     EVVLGDRVSIGPNCVLRDVRIAADTRIEAYCHFDSADIGSNCRIGPYARLRPGTQLAE
                     AVHVGNFCEVKASTIGAYSKANHLSYIGDSEVGRNVNVGAGTITCNYDGANKHRTVIE
                     DDVFIGSDSQLIAPVRVRRGATLGAGTTLTKEAPEGQLTLSRARQVSIASWQRPTKTS
                     K"
     gene            2357..4183
                     /gene="glmS"
                     /locus_tag="VKZ48_01370"
     CDS             2357..4183
                     /gene="glmS"
                     /locus_tag="VKZ48_01370"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HLU76534.1"
                     /translation="MCGIVGAVARRNIVPVLLEGLKRLEYRGYDSAGMAVLNGGLRRL
                     RTIGRVAELEKLAQAEDFSGEVGIAHTRWATHGVPSERNAHPHVSGGLAIVHNGIIEN
                     HESMRGVLRERGYEFQSDTDTEVIAHLLHDKLRTEPDLLAATRAALAELQGAYAVAVV
                     AQNDPTRIVVARKGAPLLLGLGDGEYFAASDVSALLQVTQRVIYLEEGDVAELSLDGY
                     RITDEAGLDAERTVHVSELTSAAVELGPYRHYMQKEIFEQPGALANTLELVANAKSVS
                     PQLFGAEAAAVFGEVDSVLILACGTSYHAGMVARYWIESVSGMPCTVEIASEYRYRES
                     VPNARQLVVTISQSGETADTIAALQHAKALGQPHSLAICNVPESALVRNSRLRFLTRA
                     GPEIGVASTKAFTTQLGALVLLTLVLAKLRGRLDTQRETELLAALRHLPAALQQVLGI
                     EPAIEAWADRFTSCQHALFLGRGIQYPIAMEGALKLKEISYVHAEAYPAGELKHGPLA
                     LVDKNMPVIAIAPNDALLEKLKSNLQEVRARGGELYVIADQGSRFAEEEGLHVIPLSD
                     HAGLLSPILHTVPLQLLAYHVALRRGTDVDKPRNLAKSVTVE"
     gene            4324..5808
                     /locus_tag="VKZ48_01375"
     CDS             4324..5808
                     /locus_tag="VKZ48_01375"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLU76535.1"
                     /translation="MQRPATPPFTYSMPAETSDDRWGEPRRQMALIGIAAALVLLLLI
                     ALAAYRSIEGFEARMRAVAHSHDMLASIEEMFANYAQARSNWWAYMASADADARREQF
                     EQAAASVEQSLDALLIATERPTQRARLLALNELVRGELSELRAMAAAHGDDPFAPDAD
                     FMYHILMQFGRVRDLQVALVTAERELLRSRQAESDSAGTAIRVALVGGYGLCFLVLAA
                     SLLYLHREALARRRAQREVMDLNRALHERAGALQAINKELEDFSYSVSHDLRAPVRAI
                     SSFAHIIEEEYGDRLDAEGLRLLAVVRNNAERMGRLIDDLLAFSRVGRQALNWTPVDM
                     NALVQEVIGALRVDARDMIRARFDIGYLPGIKADASLLRQVWINLIDNALKYTSRSEE
                     PLIRIRGRINNGWAEYSIEDNGPGFDVQYADKLFEVFQRLHHAPEYPGTGVGLAIVKR
                     IVDRHGGWVRAKSEPGVGACFTFALPVQEGGEHEQGNRHPAGGG"
     gene            5771..6208
                     /locus_tag="VKZ48_01380"
     CDS             5771..6208
                     /locus_tag="VKZ48_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007731113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLU76536.1"
                     /translation="MNREIDILLVEDNDADAELSLRAFRRQHLDVRVRRSRDGLDALA
                     YLRRQGAYAGRIGRQPRLVLLDIRMPFISGIEVLQQIRTDQALRTLPVVMMTSSDEAR
                     DVEAAYRLGANSYVVKPLRFHDFMRAAEEVGVYWARLNEAVET"
     gene            6334..7200
                     /locus_tag="VKZ48_01385"
     CDS             6334..7200
                     /locus_tag="VKZ48_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain N-acyl amino acid synthase"
                     /protein_id="HLU76537.1"
                     /translation="MKERLLKLFGKPGRHKADSAATARHEAADAGAGRSGETVFAAET
                     VDRGLRFRSLTLPEAEQPSIRLEAPTQPAFKIRVARTTGFRRAAGGLVGRRYGDRGYL
                     IPNTPSEPDLYSFLAYDEGQIVGTVSIRLDSSRGLSADDLYHEELEALRRSGWKICEF
                     TRLAVDVKTASKPVLAGLFHTAYLYAARIRGYDYAVIEVNPRHVAFYRRALGFEPIGP
                     ERMNRRVDAPAVLLCVAFDTIAKGVAEYGGQAERATQVRSLFPFGFGPDEEAGILQRL
                     RELDATRNGPPK"
     gene            complement(7312..8070)
                     /locus_tag="VKZ48_01390"
     CDS             complement(7312..8070)
                     /locus_tag="VKZ48_01390"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HLU76538.1"
                     /translation="MRILLITGLAAVALAAGVAEAQEPAAGKMPDAQTIVAKADEVRF
                     PDEGFEVVVDIDSKLSGGNTESRKYKVLSKGNAHTIVMVVEPASERGQIMLMRERDLW
                     IFLPNVSQPVRIGLAQRLTGQVANGDLARANFAGDYTPKLVRTDEIDGHAHYVLELTA
                     VDRGVTYGKVMYWVRQSNFWPHKAEFYSLSGRLLKTAHYRGYQQMAGRTRPTEMLMED
                     ALRKGEQSTLRYSAMKPRDLPDKIFTKDYLKRLE"
     gene            8113..8877
                     /locus_tag="VKZ48_01395"
     CDS             8113..8877
                     /locus_tag="VKZ48_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLU76539.1"
                     /translation="MTLVRVENVSKVYDLGAQRVHALRDVSLSIEAGVFLAIAGPSGS
                     GKSTLLNLIGCIDTPTSGKILIDGQDVSGRTPDQLADLRARTIGFVFQTFNLLPVLSA
                     RENIEYPLLQLKELSREERRRRVEHFLDVVQLSKYGDQRPNQLSGGQRQRVAIARALA
                     THPKIVLADEPTANLDHKTGEGILALMKDINLAFKTTFIFSTHDKKVMAKADRLVRVE
                     DGQIRALGVRRSRWLMVDELRRRERVPQTPPPIENA"
     gene            8905..10389
                     /locus_tag="VKZ48_01400"
     CDS             8905..10389
                     /locus_tag="VKZ48_01400"
                     /inference="COORDINATES: protein motif:HMM:NF018662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1302 family protein"
                     /protein_id="HLU76540.1"
                     /translation="MPTLLSAACLALPCAAAHAAGDGLILAQTSRDELFGVTPAAPPP
                     ASAPATRDELFGTTPDAPAEREAAPTREGLFGLDAPQTPAPTADRGIRWGGFYQGQLA
                     YTHSHPGHWSRAVNRLQLEGRGELAPGVRFKISARADVDPLYYETNFYPERVQDDQDF
                     NFLLRENYLDFSVGDWELRIGRQHIVWGEVVSLYFADVVSARDQRDFILPTFDITRIP
                     QWAARAEYFGDNWHAELVWIPFQSYDEIGKPGAEFYPVGRIADELPVAVFASDDRPAR
                     NISNSAWGVRMNTLVNGWDVAGFYYRSHSAMPTFHREIALVNGAPTAVFTPRHERIWQ
                     AGGTVSKDFGPFVLRGEAVYTDGQKYSVDTLADADGVVERNSFDYILGFDIAMPADTQ
                     LNVQAFHRHIFGDADDIVPKSDGFGASVLISHKLTPKWEPQLLWVQSLSHGGDRMLRP
                     RLNWYVTQNATVAFGVDIFAGPDDGFFGRFNNRDRVYAELRYDF"
     gene            complement(10561..11286)
                     /locus_tag="VKZ48_01405"
     CDS             complement(10561..11286)
                     /locus_tag="VKZ48_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76541.1"
                     /translation="MHISRYADIHRLENNPYSSAGTASEQWQHGKPVILTPASFRQCV
                     LEDVQCDLRATIADSRTEFGQARALVAQRYTARGYASASDAGRPNKEAQAVTILARRQ
                     GETVGTLTLRFDSTKGLKVDEAYGPEVGAVRDAGGVACELTCLAVSSAQDSQAILAVL
                     FRMAYYVADQLGVTDVFIEVNPRHERFYRRVLDFAVAAEERLCPRVQAPAVLLHQDMA
                     TLGKRLGGFPPPTRYGARELAAA"
     gene            complement(11546..12769)
                     /locus_tag="VKZ48_01410"
     CDS             complement(11546..12769)
                     /locus_tag="VKZ48_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLU76542.1"
                     /translation="MNTQTFTLALRNILRHKMRTAMTLAAIVIGVSGIILTGGFVQDI
                     YAQLGEAFIHSQSGHLQVAKPAYFGTGSRRPEAFLIEQPEALKRRIEAQPGVAYTMGR
                     LSFQGLLNNGRTDFAVIAEGIEPEQEARLGTSLHYIAGEAFAADDPYGLVIGEGVAQT
                     LDLAPGDPVTLVVSTPDGAMNTLDFNVSGVFRSFSRDYDARAIRLPLGAAQDLLATPG
                     VSVVVVALHRTEDTESVSGHVGDAVRDAGLAVRDWRQLNDFYEKTVALYDRQFGVLQL
                     IILIMVLLSVTNTVNMAVFERQGEFGTMRALGNRGRDVFRLIVTESLLLGAIGAVLGI
                     LCGVLLALLISAIGIPMPPPPSSNVGYTAFIRLAPDVVAGAGLVGLLAALLASLLPAW
                     RVAGTPIVDALRQNV"
     gene            complement(12779..14074)
                     /locus_tag="VKZ48_01415"
     CDS             complement(12779..14074)
                     /locus_tag="VKZ48_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLU76543.1"
                     /translation="MVDGQPADATIVPTRRLYDLPALLTDLFIALRNVLRHRRRSLMG
                     IASIAFGVIALMQAAGFVEWVYWAMREDTIRSRLGHIQIMREGYLQRGQADPYAYLLP
                     GDSPIPGEIAQLPGVRAVAPRLAFSGLASLGETTLSFIGEGMEPASEAWLNEGTVITA
                     GRDLTAADAQAMVLGHGLAQNLGASVDDTVVLLANTASGGINAVEGPVRGTFSTVSKS
                     YDDAALRLPLALAQQLLRVEGAHRWIVVLEDTAQTDDVLAQLRARYGSGDAMRLDFVP
                     WYELADFYNKTVSLFSKQVGVVKLIIAIIIVLSISNTLTMSVLERTSEIGTTLALGST
                     RRQILRRFLGEGLALGILGGVVGLALGALLALLISAIGIPMPPPPGMARGFTGEIRLT
                     PQLAFDAWTLAVVTTLAASVYPAWRASRLEIVDALRHAR"
     gene            14097..14960
                     /locus_tag="VKZ48_01420"
     CDS             14097..14960
                     /locus_tag="VKZ48_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HLU76544.1"
                     /translation="MSAFDYDAAFTRNIGWLTPAEQQKLRRARVAIAGLGGVGGFHLL
                     TLVRLGIGAFTLAEFDRFGLANFNRQAGAMMSTVGRPKLDVMIDMARDINPELDIRTF
                     PEGVQPHNIDEFLRDVDVYVDGLDFFAFAARRMTFGACARLGVPATTAAPIGMGVALL
                     NFLPDGMSFEDYFRLEGLSEAEMALHFLAGLTPAMLQRSYLVHQAAVKLERGAGPSTP
                     MACQLCAGVAATETLKIILRRGKVVAAPTALHFDAYRCRYVRTWRPGGNANPLQKLLM
                     RLIARQLARRG"
     gene            14973..16031
                     /locus_tag="VKZ48_01425"
     CDS             14973..16031
                     /locus_tag="VKZ48_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76545.1"
                     /translation="MNTTGMLTQDAIRDILEAGVWAPSAENRHAFRYSIEGDDVRLHV
                     DAAFKQGSADQQYVTLLSLGAVIENMRIAASVHALSCTVRREGEAIDADIVLHFTPAS
                     LPADELAAALKQRHTNRRPFYHGPRLSAAEQGEIEAGIVPLGDQWLVRWFDEEHGRAR
                     LARLMREAETHRFANAELHQAMFGGIRFDVGWSAGANEGLPPAALHIEPGTRAAFAAL
                     RRWSFMRTLVRVGAHHAIAMRASYIPARRSPHLCVVASRTPVHDADELLQAGCAMERV
                     WLAATRLNLAVQPMAAAVALARPGRSALTAQQRESLVAGWRALLPDAQPVMIFRFGRA
                     APPAVRAGRPLPSSLARR"
     gene            16476..17108
                     /locus_tag="VKZ48_01430"
     CDS             16476..17108
                     /locus_tag="VKZ48_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system exopolysaccharide export
                     protein"
                     /protein_id="HLU76546.1"
                     /translation="MSAALRFIYRRQLALLALFGAALLAGCGTGIGYPPAPTVAATPD
                     YRYVVGPGDQVSIIVWRNPELSLAVPVRPDGLITTPLVEDLQAAGKDPTTLARDIEQA
                     LSKYIRDPIVTVIVTNFVGPYSEQVRVIGEAGKPQTLPYRQNMTVLDVMIAVGGLTDF
                     ADGNSAVLLRTSEGGKQYTVRLKDLVKRGDVTANADVKPGDVLIIPQSWF"
     gene            17262..18821
                     /locus_tag="VKZ48_01435"
     CDS             17262..18821
                     /locus_tag="VKZ48_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide chain length
                     determinant"
                     /protein_id="HLU76547.1"
                     /translation="MDELLQQLMTYLRGMWRRRWPGLAVAWIVGIAGAVMLFKMPDQY
                     EASARVYVDTQSVLKPLMSGLAVQPNVNQQIAILSRTLISRPNMEKLIRMADLDHNIR
                     TNRQKEQLIDSLMKKLELRGSDRQNLYTINYRDTNPAEARRVVQSLLSMFVESGIGDK
                     RKGSDTARRFIDDQIAVYEGKLREAENRVKEFKLKHMGMMGGDGNQYYEQMQRLNEEL
                     ARVNLELRAAEQVRDSYRSQLAGEEAVYMPDGTALVARGPMSEIDMRIEAQKRSLDDL
                     LRRFTEAHPDVQYTRRMIASLEAEKKERGTSDTAMSGNVGGMGPINTNPVFQQLKVAL
                     AESEANVAALQARTQELASRVEKMRASAQMLPEVEAQYAALNRDYDVQRRNYTTLVQR
                     RESATLSGEMDAAAGLADFRIIDPPSVSATPVAPNRLLMLPAVLFVALGAGLFFAFVI
                     SQALPTFHDARGLREVSGRPVLGTVSLLTNAAMNRQRRRNIALFAGGLAGLFAVYGAA
                     LMLLTVGGPRI"
     gene            18832..19755
                     /locus_tag="VKZ48_01440"
     CDS             18832..19755
                     /locus_tag="VKZ48_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated tyrosine autokinase"
                     /protein_id="HLU76548.1"
                     /translation="MSLIEKAAKRLEELKRTGVAVSEEELAAAAGAPVEHYDVSAEYT
                     PLKQQMQPEEPAGPVQYSRIVDIDLSRLAAMGMVTPEAPHSPIANEFRVVKRPLLANA
                     QGKSAVPVKNGNLIMATSALPGEGKSFSALNLAMSMAMELDSTVLLVDADVANPSLLK
                     VLGLPPARGLMDVLTNDDVSLSDVILRTNVEKLSILPAGTHHQRATEVLASDVMAQLV
                     AEMAARYPDRIIIFDSPPLLPTTESRVLATHMGQVIVIVEADITTHSNLKQALAHVEN
                     CPVVLTMLNKAVRSEVAQYYGYYGYSRAARA"
     gene            19835..21388
                     /locus_tag="VKZ48_01445"
     CDS             19835..21388
                     /locus_tag="VKZ48_01445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03016 family PEP-CTERM system-associated
                     outer membrane protein"
                     /protein_id="HLU76549.1"
                     /translation="MAQKLAHRGAGAADARRKQAVIALAVASMFVIPAAQAAKWTITP
                     SIRVQETWTNNVSLSEDGEEDWDLITEVEPGLYARGQGDRLNFVLDYRALGLLYLQDS
                     DRNRVDHRFNGKLTYEAINDFAYIDVGGTATQQFESAFGAMPIDDSSDTDNRYTASSY
                     YVAPYLRGRLFGDSTYLLRYSSIWTNAGQSSVLEDGHTNQYTGRFNTPIHTFGLALTY
                     DRNDTEYDGNSDELSTEIARAIGYWRIAPQFILQARGGYEETESLFTNRSEEIYGGGF
                     LWYPTPRTSVEAFAEHRVFGTGYLFDAAHRRRFATFRVRASKDLTTYSQNLHPAVLVE
                     LLMNDPQFIEQAQNDPVLLEQLARQRLRELGLRDTQQAFYSQQIQIQERQEASVSLTG
                     IRNNVIFTVYRLDSENFADEDNPSVALLDARFGRHIEQRGASISWTHRLTGRTRTNVS
                     LYRTHVKEEEPFNRKSTAHTLRTSINTALGPNTNAYVGARYVIFDSDEGAFHDYKEAA
                     VFVGFSHRF"
     gene            21413..22516
                     /locus_tag="VKZ48_01450"
     CDS             21413..22516
                     /locus_tag="VKZ48_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system-associated ATPase"
                     /protein_id="HLU76550.1"
                     /translation="MYESFYGLSGKPFQLNPDPAFYYASRGHKRAFAYLQYGLFQGEG
                     FIVITGDVGAGKTTLLRGLLEQLDSSRVVAAQLVSTHLDADDMLKAVAAAFGLPIKST
                     DKAALLVQLESYFVSLAAKRKRALLIVDEAQNLTPRAVEELRMLSNFQLDNHALLQSF
                     LIGQPELRETMRSPHMQQLRQRVLASYHLGPLDADETRAYIEHRLTRVGWTGDPSFDD
                     EAFAEIHRASKGIPRRINTLSNRLLMSGYLSERHRIEGGDVLTVAEEIKDELGVELSP
                     ASDVGDTRATPPAAPQGGFPRVPQASEFKGNGFKLDAEQAGIARQILSGSVLSGLEGR
                     ITNLEQTLHATLTLLQQLAHEHGEPAERTAERR"
     gene            22518..23681
                     /gene="wecB"
                     /locus_tag="VKZ48_01455"
     CDS             22518..23681
                     /gene="wecB"
                     /locus_tag="VKZ48_01455"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HLU76551.1"
                     /translation="MGDLGRYPGPIVCVVGARPNYMKAAPILRAFTAQPALPRVMLVH
                     TGQHYDIAMNERLFEDLQLPRPDLNLEVGSGTHAVQTAEVMRGFEPVLDELRPSCVVV
                     VGDVNSTLACALVAAKKRVPVVHVEAGLRSGDRRMPEEINRVLTDQIADLLYTTERSA
                     HANLAAEGVGAHRVHFVGNVMIDSLLAHRRRAVPPSETLMRAGAPLSVLDHPAGYGVV
                     TLHRPSNVDEPEALAETLQILREVSASLPLVCALHPRTRANIERFDLGQLIEGEAFVV
                     LPPQGYLEMLGLQASATVVLTDSGGMQEETTALSVPCLTLRENTERPVTIEQGTNTLV
                     GRDRRLILDTVASILRDGGKRGRTPELWDGRAAERIAAHLAQWLQRRPVRAGA"
     gene            23685..24539
                     /locus_tag="VKZ48_01460"
     CDS             23685..24539
                     /locus_tag="VKZ48_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide deacetylase"
                     /protein_id="HLU76552.1"
                     /translation="MQTARAEDFVNALTVDVEDYFQVSALAPYIPRSEWGAVDCRVER
                     NVERILQLLCENGARATFFTLGWVAERYPSLVRAIVEEGHELASHGYGHHRASDQTPE
                     AFRADIQLAKIVLEDAAGVEVKGYRAPSFSIGERNLWAFDCIAEAGYRYSSSVYPVHH
                     DHYGMPDAPRFPYEARVGLLEVPITTVRMFNANLPGGGGGYFRLMPYGLSRWLIRRVN
                     FEEGRSAIFYFHPWEIDPEQPRVKGVKFKARFRHYVNLRRTEKRLRRLLRDFNWDRVD
                     KVFLGAQA"
     gene            24575..25597
                     /locus_tag="VKZ48_01465"
     CDS             24575..25597
                     /locus_tag="VKZ48_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family XrtA/PEP-CTERM system-associated
                     protein"
                     /protein_id="HLU76553.1"
                     /translation="MVRACTPDDAERWESFVERCPNASFFHRLGWKTVLEEVFRHRTH
                     YLLAERDGGVVGVLPLAEVKSALFGHALTSLPFAVYGGVAATDAQAVSALHRRARDIG
                     AELGVQHLELRNREVREADWPRQDLYVTFRKDILPDVETNLLAIPRKQRAMVRKGIGN
                     GLASHIDADTGRFFSLYADNVRRHGTPALPRRYFETLRTVFGDDCEVLTVTDTQGTPL
                     SSVLSFYFRDEVLPYYAGDHVRARELAANDFKYWELLRRACQRGYKVFDYGRSKRGSG
                     SYAFKKNWGFSATPLAYEYCLYKRDRIPQNNPNNPKYRAFIALWRRLPMPVANALGPF
                     IVRGLG"
     gene            25619..27184
                     /gene="xrtA"
                     /locus_tag="VKZ48_01470"
     CDS             25619..27184
                     /gene="xrtA"
                     /locus_tag="VKZ48_01470"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase A"
                     /protein_id="HLU76554.1"
                     /translation="MHAERQIEAQLSVALRPRQYALLGVIALCLVLVFAWHGETFAAM
                     AATWWRTETFAHGLIVLPIFAWLVWRDRARLIALRIAPYPPALLALAAAGFVWMLGEL
                     SATVGVSQFAMVAMVPCALCALLGRAALRALAFPLAFLFFAVPFGAFMLPWLMQLTAD
                     FTVGAVRLSGVPVYREGMLFHLPSGSWSVVEACSGLRYLIASVMVGTLYAYLTYRSPR
                     RRLLFLGVAIAVPLVANWLRAYFIVMLGHLSDNRLAAGVDHLIYGWVFFGVVMAIMFW
                     IGTRWREDLDDTAPAKPAEPQFDAVRRAPRHALGVALAVLLLIGVWKPTAAALSMPQA
                     GPDPVLQPLAGVAAWQPEAASFTAWTPPHDTPAATLDQQFRNGDAQVGMHVAYYRNQS
                     AESEAVRGESGLLEGPDAAWRRISARHIDVDAGADPFSVMRAEFVHPLDRIVAWRWYW
                     IDGRMTSNAYVAKAQLAWAKLRGASDDSAVIVLYTPRRDASDDADRRLADFLRAMSPA
                     LLNTLHGARGGAS"
     gene            27181..28377
                     /locus_tag="VKZ48_01475"
     CDS             27181..28377
                     /locus_tag="VKZ48_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03088 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HLU76555.1"
                     /translation="MKRDAAPFADSRPLVVHVVHRFAVGGLENGVANLINGMPDSRFR
                     HAVLAMTEVTDFKARIVREDVAYFELRKPAGHALRCYPQLVRMFRELQPAIVHTRNLS
                     ALEAAVPAMIAGVPVRVHSEHGRDVGDLDGSNAKYRWLRRAYSPCVHRYVALSRDLER
                     YLVDRVGIAPYRVAQIYNGVDTVRFRPRESGRVPVEGSPFNDPRYWVVGTVGRMHVVK
                     DQLLLVRAFLQTLATVPEARERLRLMLVGDGPLRAEAEALLREAGASSCAWLAGERAD
                     VPELLRAMDCFVLPSLAEGISNTILEAMASGLPVIATRVGGNPELVIDQVTGTLIPSG
                     DVAALVAAINGYYALPDVAAEHGRAGRARVERKFSLDYMTHRYESLYTSLLGDRPATR
                     AGLKTT"
     gene            28389..>30181
                     /locus_tag="VKZ48_01480"
     CDS             28389..>30181
                     /locus_tag="VKZ48_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system amidotransferase"
                     /protein_id="HLU76556.1"
                     /translation="MCGLVGIFDTRGRRDIARPVLERMNESQRHRGPDEDGIHLEPGL
                     GLGHRRLSIIDLSSGRQPIYNEDGTVAVVYNGEIYNYRALIAELTALGHRFRTRSDSE
                     VIVHAWEEWGEGCVERFRGMFAFALWDRRRETLFLARDRLGVKPLHYALLPDGQLLFG
                     SELKSLLAHGGLARDIDPCAVEEYFALGYVAEPRSIFKMAQKLPPAHTLCIRRGTPIP
                     APHAYWDVRFTLDNRIGAADAQAELEARLRESVSLRLMSEVPLGAFLSGGVDSSAVVA
                     MMAGVSDEPVNTCSIAFNEPAFDESAYARQVAERYRTNHYVEQVDSDDFDLIDTLAGL
                     YDEPYADSSAIPTYRVCQLARKRVTVALSGDGGDESFGGYRRYRLHMAEERLRGALPL
                     AFRRPLFGLLGRVYPKADWAPRVLRAKATFQALSHNAVEAYLTSVSLLRDAPRRQLYS
                     GSFRAQLGGYTAHEVFHRHAAHADADDPLALIQYLDLKTYLVGDINTKVDRASMAHSL
                     EVREPLMDHPLVEWLATLPSSFKVRGQEGKYLLKKAMEPHLPKEILYRSKRGFAVPLA
                     RWFRGPLRARVRQAVLSERLLDTGFFNRVQL"
BASE COUNT         5263 a         9874 c         9819 g         5225 t
ORIGIN      
        1 gccgccggcg acatgcgccg cggccagtca ctcatcgtct gggcgatccg cgaaggccgt
       61 caatgcgcgc ggtcggtgga tgaatacctg atgggcgcgt ccaccctgcc gcgctagtca
      121 aggcaggcgg tgcacttcgc gcgcgcgttt gggcggcgcc ggcggcgcca ccgcctgccc
      181 gccgatgccg ccggggcccg gctttatcac gtagcgcggt atgaagcgga tattggtacg
      241 gcggctgttg ccgtcggcat ccaggtcgcg gtcgatgatc acttcaccga gcggcggctc
      301 ggcatgtccc ccgatccaac cgcttgccgg gtaataaacg ccgctgcctt ccgccggcgc
      361 ataggcgacg tccgacgagc gccgcgcggc gcgtagtgcc tcttccaact gattgatccg
      421 ccggttctgc agccgttcgt tcaaggcgcg cacttcctct gccgacggtt tcggcaacgt
      481 gacgatgctc agccggttct ccggcacgcg ctcggccgag cgcacgtcgc gcggcgctac
      541 gttgctgtag tgcaccacgc cctgcgcatc cgtccatttg taaacttctg caccaaccgg
      601 cgcggcgacg gcaaatgtga gcacacttgc ggcgaagact ccggcactgc gatggacggt
      661 cgagcgaaac atggctgcct ccgatgtcga tacggcgtac acagggcggg atttgtcctg
      721 catgtggcaa agcccgtgcc ttcgggcggt gaaaggtgca aatacggcgt gctaccatcc
      781 cccgtcgctg cagacacggc atgactccgg tgctcataag actccgatta aggctgtttg
      841 atccatgttg cgagctcgtg tcgttcaagc gcaatctgac acggctttgc ggccatgatc
      901 gtctccggtg tgtaatcaga ctttcccatc gccccaatga acatcgtcat tctcgccgcc
      961 ggacagggta aacggatgca ttccgaccgt ccgaaggtcc tgcacgaact ggccggccgc
     1021 ccgctgcttg cgcatgtcgt cgagacggcg cgtgcgctcg gccccgaccg gatctgcgtg
     1081 gtgtatggcc atggcggcga agcggtgcgc gaggcgatcg gcgaagacgg attgcaatgg
     1141 gcgctgcaaa acccgcaact ggggacgggc catgccgtcc agcaggcgct gccgctgctc
     1201 ggtaagcagg ggcctactct cgtgctttac ggcgacgtgc cgctcatttc cgccgacagc
     1261 ttgcgtccgc tggtggaagc cgcccgcgac ggcatcggtc tgctgacggt ggagatggac
     1321 gatccgaccg gttatgggcg catcgtgcgc gaaggcggtg cggtgcggcg catcgtcgaa
     1381 cacaaagacg cgacgcccga acagcgtcag ttgcgcgaag tgaacaccgg catcatggcg
     1441 gtgccgaccg accggctggc cgcctggctc gccgccctgc gcaacgacaa cgcgcagggc
     1501 gagtactacc tcaccgacat catcgcgcag gcggtcgccg aaggcctgcc ggtgcgcgcg
     1561 ttccagccga ccgctgcagc cgaaacgttg ggcgtcaaca gcaagacgca gctggccgaa
     1621 ctggagcgaa tctaccagcg caaccgcgcg gctgccttgc tggagcaggg cgtgacgctc
     1681 gccgaccccg ggcgcttcga tgtgcgcggc acgctgcgct gcggacggga cgtctatatc
     1741 gacgtcaatt gcgtattcga aggcgaagtg gtactcggcg accgcgtttc catcgggccc
     1801 aactgcgtgt tgcgcgatgt gcgcatcgcc gccgatacgc gcatcgaagc ctactgtcac
     1861 ttcgactcgg ccgacatcgg cagcaactgc cgcatcggcc cctacgcccg gctacggccc
     1921 gggacgcagc tcgccgaggc ggtgcatgtg ggcaatttct gcgaagtgaa agccagtacg
     1981 atcggtgcct attccaaggc gaatcatctg agctacatcg gcgacagtga agtgggccgc
     2041 aacgtcaatg tcggcgccgg caccatcacc tgcaactacg acggcgccaa caagcatcgc
     2101 accgtcatcg aggacgacgt gttcatcggt tccgactcgc agttgatcgc gccggtgcgg
     2161 gtgcgtcgcg gtgcgacgct gggcgccgga acgacgctca ccaaggaggc gccggaaggc
     2221 cagttgacgc tgtcgcgcgc gcggcaggta tcgatcgcat cctggcagcg gccgacaaag
     2281 acatccaaat gattcatcgt gacaacggcc gcgcgccgcg ctgcgcgcca gtgcaaccct
     2341 gaatagtgtg tagattatgt gtggcatcgt cggcgccgtc gcacggcgca acatcgttcc
     2401 ggtactgctc gagggactga agcggctcga gtaccgcggc tacgactcgg ccggcatggc
     2461 cgtgctcaac ggcggattgc gccggctgcg gaccatcggc cgcgtcgccg aactggagaa
     2521 gttggcgcag gccgaagact tcagcggcga agtcggcatt gcgcacacgc gctgggccac
     2581 gcatggcgtg ccctccgagc gcaacgcgca tccgcacgtc agcggcggcc ttgccatcgt
     2641 gcacaacggc atcatcgaaa atcacgaaag catgcgcggt gtgctgcgcg agcgcggcta
     2701 cgagtttcag tccgacaccg acaccgaagt catcgcccat ctgctgcacg acaagctgcg
     2761 taccgaaccc gatctgctgg ccgccacgcg cgccgcactc gctgaattgc agggtgccta
     2821 tgccgtcgcc gtggtggcgc agaacgatcc gacccgcatc gtggtggcgc gcaagggcgc
     2881 gccgcttttg ctcggcctgg gcgacggcga gtacttcgcc gcttccgacg tttccgcgct
     2941 gctgcaggtg acgcaacgcg tgatctacct ggaggaaggc gatgtcgcgg agttgagtct
     3001 cgacggctat cgcatcaccg acgaggcggg gctggacgcg gagcgcaccg tccatgtgag
     3061 cgaactgacg tcggctgccg tggaactcgg gccgtaccgg cactacatgc agaaggagat
     3121 tttcgagcag ccgggcgcgc tggcgaacac gctggaactg gtggccaacg cgaaatccgt
     3181 ttccccgcag ttgttcggtg cggaagcggc ggcggtgttc ggcgaagtcg attcggtgct
     3241 gattcttgcc tgcggcacga gctatcacgc cggcatggtg gcgcgttact ggatcgaatc
     3301 ggtgtccggc atgccatgca cggtggagat cgcgagcgag taccgctatc gcgaaagcgt
     3361 gcccaatgcg cgacagctgg tggtgaccat ttcgcaatcg ggcgaaacgg cggacaccat
     3421 tgccgccctg cagcatgcca aggcgttggg tcagccgcat tcgctggcga tctgcaacgt
     3481 cccggaaagc gcactggtgc gcaactccag gctgcgcttc ctcacgcgcg cgggtcccga
     3541 gatcggcgtg gcgtccacca aggccttcac cacgcagttg ggcgcactgg tgctgctgac
     3601 cctggtgctg gccaagctgc gtggacgcct cgacacacag cgcgaaaccg aactgctggc
     3661 cgcgctgcgc catctgcccg ccgccctgca gcaggtgctg ggtatcgagc cggccatcga
     3721 agcctgggcc gaccgcttca cctcctgcca gcacgcgctg ttcctcggac gcggcatcca
     3781 atatccgatc gccatggagg gcgcgctgaa gctgaaggaa atctcctacg ttcatgccga
     3841 agcctatccg gccggcgagt tgaagcacgg tccacttgcg ctggtcgaca agaacatgcc
     3901 ggtcatcgcc atcgcaccga acgacgcgct gctggaaaag ctcaaatcca atctgcagga
     3961 agtgcgcgcg cgcggcggcg aactctatgt gatcgccgat cagggttcgc gctttgccga
     4021 ggaggaaggc ttgcatgtca ttccgctcag cgatcacgcc ggtcttctgt cgcccatact
     4081 gcacaccgtt ccattgcaac tgctggccta ccatgtcgcg ctgcgtcgcg gcaccgacgt
     4141 cgataagccg cgcaacctcg ccaaatcggt tacggtcgaa tagcgcggtg cgcgcggtcg
     4201 ccgatgacaa gcagcgcatg cgttgcccat cggcagtcgc gttgcgagtt ctgccgttgc
     4261 ggagaatccg aggaggcgcg tcgtcgctgc ggcggcgtcc attgcgttag cggacactag
     4321 ttgatgcaac gccccgccac gccgccgttt acctacagta tgccggccga gacatcggac
     4381 gaccgctggg gtgagccgcg tcggcagatg gcgctgatcg gcatcgccgc cgcgctggtg
     4441 cttctgctgc tgatcgccct cgctgcatat cgcagcatcg aaggcttcga agcgcgcatg
     4501 cgcgcggtcg cccactcgca cgatatgctg gcgagcatcg aggagatgtt cgccaactac
     4561 gcgcaggcgc gcagcaactg gtgggcgtac atggcgagcg ccgacgccga cgcgcggcgc
     4621 gaacaattcg aacaggcggc ggcgagcgtc gaacagagtc tcgacgcgtt gctgattgcg
     4681 accgagcggc ccacgcagcg tgcgcgactg ctcgcgctca atgaactcgt gcgcggcgag
     4741 ttgtcggaat tgcgtgcaat ggcggcagcg catggcgacg accccttcgc gcccgacgcg
     4801 gatttcatgt accacatcct gatgcagttc ggccgtgtgc gcgatctgca ggtggcgctg
     4861 gtcaccgccg agcgcgagtt gctgcgtagc cgtcaggccg aatccgacag cgccggcacg
     4921 gcaattcgcg tcgcgctggt gggcggctac ggcttgtgct ttctcgtgct ggcggcctcg
     4981 ctgctgtatc tccatcgcga agccctggcc cgtcggcgcg cgcagcgcga ggtcatggat
     5041 ctcaatcgtg cgctgcacga acgcgccggc gccctgcaag cgatcaacaa ggaactcgag
     5101 gatttctcct attcggtgtc gcacgatctg cgtgcgccgg ttcgcgccat ctccagcttt
     5161 gcccacatta tcgaagagga atacggcgac agactcgatg ccgaaggctt gcgcctgctg
     5221 gccgtagtgc gcaacaacgc ggagcgcatg ggacgcctga tcgacgatct gctcgcgttt
     5281 tcgcgcgtcg gccgtcaggc gctgaattgg acgccggtgg atatgaatgc gctggtgcag
     5341 gaagtcatcg gcgcattgcg cgtcgatgcc cgcgacatga tccgcgcacg ttttgacatc
     5401 gggtacttac ccggcatcaa agcggacgcc tcattgctgc ggcaggtttg gatcaacctg
     5461 atcgataacg cgctgaaata cacaagccga agcgaggaac cgctgattcg cattcgcgga
     5521 agaattaata atggctgggc ggagtacagc atcgaggaca acgggccggg gttcgacgtg
     5581 cagtatgcgg acaagttgtt cgaggtgttc cagcgcctgc atcatgcgcc cgagtatccg
     5641 ggcacgggag tcgggttggc gatcgtcaag cgcattgtcg atcgtcacgg cgggtgggtg
     5701 cgggcgaaga gcgaaccggg ggtgggcgcg tgcttcactt tcgcgctgcc cgtgcaggaa
     5761 ggaggcgagc atgaacaggg aaatcgacat cctgctggtg gaggataacg acgccgatgc
     5821 cgaactgagt ctgcgcgcat tccggcgcca gcatctggat gtgcgggtgc gccgctcgcg
     5881 cgacggcctg gacgcgctgg cttatctgcg tcgtcagggt gcctacgccg gccgcatcgg
     5941 acgtcagcct cggttggtgc tgctcgacat ccgcatgccc ttcatcagcg gcatcgaagt
     6001 attgcagcag atccgcaccg accaggcact gcgcaccctg cccgtcgtaa tgatgacctc
     6061 ctccgacgag gcgcgcgatg tggaggcggc gtaccggctg ggcgccaaca gctatgtggt
     6121 gaagccgctg cgctttcacg atttcatgcg cgccgccgag gaagtcggcg tgtactgggc
     6181 ccgcctgaac gaggcggtcg aaacctgaac gccgcccgcg cgcctcatcc tggccggttg
     6241 cttgctttac cacaccggat aaccgcccgg ccgttttgag gaaccggaac gatcccgggc
     6301 aggagtgtgg cgatgcgcgg ggtggcgctg gagatgaaag aacgattgct aaagctgttc
     6361 ggtaagccgg ggcggcacaa ggcggacagc gcggcgacgg cgcggcatga ggcggcggac
     6421 gccggtgcgg gacgcagtgg cgaaaccgta ttcgccgccg aaaccgtcga tcgcggcctg
     6481 cgctttcgca gcctgaccct gcccgaggcg gagcagccgt ccatacgcct ggaagcgccc
     6541 acgcagccgg cgttcaagat ccgcgtggcg cgcaccaccg gcttccgccg ggcggcgggg
     6601 ggcctggtcg gacggcgcta tggcgatcgc ggctatctca tccccaacac cccctcggag
     6661 cccgacctct attcctttct cgcttatgac gaggggcaga tcgtcggcac ggtgagcatc
     6721 cgcctggact ccagtcgcgg cctttcggcc gacgatctct atcacgagga actggaagcg
     6781 ctgcgccgca gcggctggaa aatctgcgag ttcacccgcc tggcggtgga tgtcaaaacg
     6841 gcgagcaagc cggtgctggc cgggctgttc cacaccgcct acctctatgc cgcgcggatc
     6901 cgcggctacg actacgccgt gatcgaagtg aatccgcgcc acgtcgcgtt ctatcgtcgc
     6961 gcgctcggct tcgaacccat cgggccggaa cggatgaacc gccgggtcga cgcaccggcc
     7021 gtgctgctgt gcgtagcctt cgataccatc gccaagggtg tggccgagta cggcggccag
     7081 gccgagcggg ccacgcaggt gcgctcgctg tttcccttcg gcttcgggcc ggatgaggag
     7141 gcgggcatcc tgcagcgcct gcgtgaactc gatgcgaccc gcaacgggcc gcccaaatga
     7201 gagggtcggt gcagtggtcg agtccgttcg atggatgcgg tcggcggtgc ggcgcgcagc
     7261 ggccgggtta cctcgtcctc gccgcgattt cccgggcgca cgccgggttc gttactccag
     7321 ccgcttgagg tagtccttgg tgaaaatctt gtccggcagg tcgcgcggct tcatggcgct
     7381 gtagcgcagg gtcgattgct cccccttgcg cagcgcgtct tccatcagca tttcggtcgg
     7441 ccgcgtccgg cccgccattt gctggtagcc gcgatagtgc gccgtcttca gcaggcggcc
     7501 ggagagggaa tagaactccg ccttgtgcgg ccagaaattg gattgacgca cccagtacat
     7561 caccttgccg taagtgacgc cgcgatcgac ggcggtgagt tcgagcacat agtgggcatg
     7621 accatcgatc tcgtccgtgc gcaccagttt cggggtgtaa tcgccggcga agttcgcgcg
     7681 ggccagatcg ccgttggcga cctgaccggt cagccgttgc gccaggccga tacgcacggg
     7741 ctgcgacacg ttgggcagga agatccacaa atcgcgttcc cgcatcagca tgatctggcc
     7801 gcgctccgag gccggctcga cgaccatcac gatggtgtgc gcgttgccct ttgaaaggac
     7861 cttgtacttg cgcgactccg tattgccgcc ggaaagcttg gaatcgatgt cgaccaccac
     7921 ttcgaagcct tcgtcgggga agcgcacttc gtcggctttg gcgacgatgg tctgggcatc
     7981 cggcattttg ccggctgcag gttcctgtgc ttcggcgacg cccgcggcga gcgccacggc
     8041 ggccaggccg gtgatcaaca aaatacgcat cggttgcaat cctcaaacga tcaacgcgaa
     8101 cagggcagcg tcatgacgct cgtgcgcgtg gaaaacgtct ccaaggttta cgatctgggc
     8161 gcgcagcgcg tgcacgcgct gcgcgacgtc agtctcagca tcgaggccgg cgtctttctc
     8221 gccatcgccg ggccgtcggg tagcggcaaa tccacgctgc tcaatctcat cggctgcata
     8281 gacaccccca cgagcggaaa aatcctcatc gacggccagg acgtcagcgg tcgcacgccc
     8341 gatcagctcg ccgatctgcg cgcgcgcacc atcggcttcg tgttccagac cttcaatctg
     8401 ctccccgtgc tgagcgcgcg cgagaacatc gagtatccgc tgctgcagtt gaaggagctg
     8461 tcgcgcgagg agcggcgtcg gcgcgtcgag cattttctcg acgtggtgca actgtccaaa
     8521 tacggcgacc aacggcccaa tcaactgtcc ggcgggcagc gccagcgtgt ggccattgcg
     8581 cgtgcgctgg ccacgcatcc caagatcgtg ctggccgacg agcccaccgc caatctcgat
     8641 cacaagaccg gcgaaggcat tctggcgttg atgaaggaca tcaacctcgc cttcaagacc
     8701 accttcattt tctccacgca tgacaagaag gtaatggcca aggccgatcg cctggtgcgc
     8761 gtcgaggatg gtcagatccg cgcgctcggc gtacgccgca gccgctggct catggtggac
     8821 gaattgcgcc gccgcgagcg cgtcccccaa acgccgccgc ccatcgaaaa cgcctgaccc
     8881 cagggagccc gaccaatgaa aatcatgcct acccttctca gcgcggcttg tcttgcgctg
     8941 ccttgcgcgg cggcgcatgc ggccggcgac ggcctgatcc tcgcgcagac ctcgcgcgac
     9001 gagttgttcg gcgtaacgcc ggccgcaccg ccgcccgcga gcgcacccgc cacgcgcgac
     9061 gaattgttcg gcacgactcc cgatgcgccg gccgagcgcg aggctgcgcc cacgcgcgag
     9121 gggctgttcg gcctcgatgc accgcagacg ccggcgccga ccgcggatcg cggcatccgc
     9181 tggggcggct tctatcaggg acagttggcc tatacccata gtcatcccgg tcactggtcg
     9241 cgtgcggtga accgcctgca actggagggc cggggcgaac ttgcgcctgg cgtgcgcttc
     9301 aagatctccg ctcgcgccga tgtcgatccg ctctattacg agaccaattt ctatcccgag
     9361 cgcgtgcagg acgaccagga cttcaatttc cttctgcgcg agaactatct ggatttctcg
     9421 gtgggcgact gggaactgcg catcggccgc cagcacatcg tgtggggcga agtggtcagc
     9481 ctgtactttg ccgacgtcgt ctcggcgcgc gaccagcgcg acttcatcct gccgaccttc
     9541 gacatcaccc gcattcccca atgggccgcg cgcgccgaat acttcggcga caactggcac
     9601 gcggaactgg tgtggattcc ttttcagagc tacgacgaga tcggcaagcc gggcgcggag
     9661 ttctatcccg tcgggcgcat cgccgacgaa ctgccggtag cggtcttcgc ctccgacgac
     9721 cggccggcac gcaacatctc caactccgcg tggggcgtgc gcatgaacac gctggtcaac
     9781 ggttgggacg tggcgggttt ctactaccgc agccacagcg cgatgccgac cttccatcgc
     9841 gaaatcgcgc tggtgaacgg tgcgccgacg gcggtgttta cgccgcgtca cgaacgcatc
     9901 tggcaggcgg gcggcacggt gagcaaggat ttcggcccct tcgtgctgcg cggcgaagcg
     9961 gtctataccg acgggcagaa gtacagcgtc gacacactgg ccgacgccga cggcgtggtg
    10021 gagcgcaaca gtttcgatta catcctcggc ttcgatatcg ccatgccggc ggatacgcaa
    10081 ctcaatgtgc aggcattcca tcgacacatc ttcggcgacg ccgacgacat cgtgccgaag
    10141 tccgacggct ttggcgccag cgtgctcatc agccacaaac tcactcccaa atgggaaccg
    10201 caactgttgt gggttcagag cctgtcgcac ggcggcgatc gcatgctgcg cccgcgcctg
    10261 aactggtacg tcacgcagaa cgccacggtg gcgttcgggg tggacatttt cgccgggccg
    10321 gacgacggtt tcttcggccg cttcaataat cgcgatcgcg tctacgccga gttgcgctac
    10381 gacttctaaa aaatcaaaag ctgaaacaca gagaacacgg agaccacaga gaagaccttt
    10441 gaagcgggat tttctctgtg tcccccgtgg ttcaagattt ttacagggcg ttcgcaggcg
    10501 gtacacaaaa aaacgggcga tcttgcgatc gcccggtcgc gaagggccag ggagggggag
    10561 ctaggctgcc gcgagttcgc gcgccccgta ccgggtcggg ggagggaaac cgcccagtcg
    10621 tttaccgagt gtggccatgt cctgatgcag caacacagcc ggtgcctgta cacgcgggca
    10681 caggcgctcc tcggccgcta cggcgaaatc gagcacgcgc cggtaaaagc gctcgtgccg
    10741 gggattgact tcgatgaaga cgtcggtcac gccgagctgg tcagcgacgt aataggccat
    10801 gcgaaacagc accgcgagga tcgcctgcga gtcctgcgcc gaagatacgg ccaggcaggt
    10861 cagttcgcag gcgacgccgc ccgcgtcgcg cactgcgccg acttcggggc cgtaggcttc
    10921 gtccaccttc aagcctttgg tggagtcgaa gcgcagagtc agggtgccga ccgtctcgcc
    10981 ctgacgccgg gccaggatgg tcacggcctg cgcttccttg ttgggacggc ccgcatcgct
    11041 cgccgaggca tagccgcgtg cggtatagcg ctgcgcgacc agcgcgcgcg cctgcccgaa
    11101 ctcggtgcgg ctgtcggcga tggtcgcgcg cagatcgcac tgcacgtctt ccagtacgca
    11161 ctgcctgaac gaagccgggg tcaggatgac gggtttgccg tgttgccatt gctcgctcgc
    11221 cgtgccggca ctgctgtaag ggttgttttc gaggcggtgg atatcggcgt agcgagagat
    11281 atgcatgaga ggctttgtcc tgcggtgtgg cgtttgtaaa aaatactgca cgcgctcttt
    11341 gagcaaggct tgggccaccg caaaaatatg ttgctaaaca atgggttgcg atttgtccta
    11401 cagagcaggt aggaatttac cgtctccttg cggcgacgat gcacggggca gaatgactaa
    11461 cggcctattt ttaaagggct agttgcagtc gtgatatggc gacatagaca aagccgtctc
    11521 ggaaaaatcc gaccgcgccg tggcgctata cgttctgacg cagcgcgtcg acgatgggcg
    11581 ttccggccac gcgccatgcg ggcagcaggc tggcgagcag cgccgcgagc aatcccacca
    11641 ggccggcgcc cgcgaccaca tccggcgcca gacggatgaa ggcggtatag ccgacattgg
    11701 agctgggcgg cggtggcatg gggatgccga tggcggaaat cagcagcgcg agcagcacgc
    11761 cgcaaaggat gccgagcacc gcgccgatcg cccccagcag caggctttcg gtcacgatca
    11821 ggcggaacac gtcccggccg cgattgccca gcgcgcgcat ggtgccgaac tcgccctgcc
    11881 gctcgaacac cgccatgttg acggtgttgg tgacgctcag cagcaccatg atcaggatga
    11941 tgagttgcag cacgccgaac tgccggtcgt acagcgccac ggttttttcg tagaagtcgt
    12001 tgagctgccg ccagtcgcgc accgccagac cggcgtcgcg caccgcgtcg ccgacgtgcc
    12061 ccgagacgct ttccgtgtct tccgtccgat ggagcgcgac gacgacgacg ctgaccccgg
    12121 gcgtggcgag cagatcctgc gccgcgccca gcggcagccg gatggcgcgc gcgtcatagt
    12181 cgcgcgagaa ggagcggaac acgccgctca cgttgaagtc gagcgtattc atcgcgccgt
    12241 ccggtgtact caccaccagt gtgacgggat cgcccggcgc aagatcgagg gtctgtgcca
    12301 cgccttcgcc gattaccagg ccgtacggat cgtccgccgc gaaggcctcg ccggcgatgt
    12361 aatgcaggga ggtgcccaga cgcgcctcct gttcgggttc gatgccttcg gcgatcaccg
    12421 cgaaatcggt gcgaccgttg ttgagcaggc cctggaagct cagccgaccc atggtgtagg
    12481 ccacgcccgg ctgcgcttcg atccgccgct tcagcgcctc gggctgttcg atcagaaagg
    12541 cttcgggccg tcgcgaaccg gtgccgaaat acgccggctt ggcgacctgt agatgcccgg
    12601 actgcgagtg gatgaacgcc tcgcccaact gcgcgtagat atcctgcacg aaaccgcccg
    12661 tgagaatgat gccgcttacg ccgatgacga tcgccgccaa tgtcatggcg gtgcgcatct
    12721 tgtggcgcag gatgttgcgc agcgcgagcg tgaaggtctg cgtgttcatc gcttgccgct
    12781 agcgcgcgtg ccgcagcgca tcgacgatct ccagtcgcga tgcgcgccag gcgggataaa
    12841 cggacgcggc gagcgtcgtc accaccgcca acgtccaggc gtcgaaggcc agttggggcg
    12901 tgaggcggat ctcgccggtg aagccgcgcg ccatgcctgg cggcggcggc atggggatgc
    12961 cgatggcgga aatcagcagc gcgagcagcg cgccgagcgc aaggcccacc accccgccga
    13021 gtatgcccag cgccagcccc tcgccgagaa agcgccgcag aatctgccgt cgcgtcgaac
    13081 ccagcgcgag cgtggtaccg atttcgctgg tgcgttccag cacgctcatg gtcagcgtat
    13141 tggaaatgct gagcacgatg atgatggcga tgatcagctt caccacgccc acctgtttgg
    13201 agaacaggct gaccgtcttg ttgtagaagt cggccagttc ataccaggga acgaagtcga
    13261 gccgcattgc gtcgccgctt ccgtagcgtg cgcgcagttg cgcaagcacg tcatccgtct
    13321 gcgccgtgtc ttccagcact acgatccagc gatgcgcgcc ttcgacgcgc agcagttgct
    13381 gcgccagcgc cagcggcagc cgcaacgccg catcgtcgta gctcttcgat accgtggaga
    13441 aggtgccacg caccggtcct tccaccgcgt tgatgccgcc cgaggcggtg ttggccagca
    13501 gcacgacggt gtcgtccacg cttgcgccga ggttctgcgc cagaccgtgt cccagcacca
    13561 tggcttgcgc gtccgctgcc gtgagatcgc gtccggcggt gatgaccgtg ccttcgttca
    13621 gccacgcttc gctggccggc tccatgcctt cgccgatgaa ggacagcgtc gtctcgccca
    13681 ggctcgccag gccggaaaag gccagacgcg gggccaccgc gcgcacgccc ggcagttggg
    13741 cgatctcgcc cggaatgggc gaatccccgg gcagcagata ggcgtagggg tcggcctggc
    13801 cgcgctgcag atagccctcg cgcatgatct ggatatggcc caggcgggag cggatggtgt
    13861 cttcgcgcat ggcccagtac acccattcca cgaagccggc cgcctgcatc agggcgatca
    13921 cgccgaaggc gatggaggcg atgcccatga ggctgcggcg gcggtgccgc agcacgttgc
    13981 gcagggcgat aaaaaggtcg gtcagcaagg cgggcaggtc gtacaggcgg cgtgtcggca
    14041 cgatagtggc atcggccggc tggccgtcaa ccatcgggcc tttcgcgggc gattcagtga
    14101 gtgcattcga ttacgacgcc gcctttacgc gcaacatcgg gtggctaacg ccagccgaac
    14161 agcaaaagct gcgccgtgct cgggtggcca tcgccgggct gggcggcgtc ggcggctttc
    14221 atctgctcac gctggtgcgg ctgggcatcg gcgccttcac cctggccgag ttcgaccgct
    14281 tcggactggc caacttcaac cgccaggccg gcgcgatgat gtccaccgtc ggccggccca
    14341 agctcgacgt gatgatcgac atggcgcgcg acatcaaccc cgagctcgat atccggacct
    14401 ttccagaagg cgtgcagccg cacaacatcg acgaattcct gcgcgacgtc gatgtgtacg
    14461 tggacggcct ggacttcttc gccttcgccg cgcgacgcat gaccttcggc gcctgtgcgc
    14521 gattgggcgt gcccgccacc accgccgcgc ccatcggcat gggcgtcgcg ctactcaact
    14581 tcctgccgga cggcatgagc ttcgaggact attttcgtct cgaggggctg tccgaggccg
    14641 agatggcgct gcacttcctg gccggactga cacctgccat gctgcagcgc agttatctgg
    14701 tgcatcaggc ggcggtgaag ctcgagcgcg gcgcagggcc gtcgacaccg atggcctgtc
    14761 agctgtgcgc gggcgtggcg gccaccgaga cgctgaaaat cattctgcgg cgcggtaaag
    14821 tggtcgccgc gcccaccgcc ctgcacttcg acgcctatcg ctgccgctat gtgcgcacct
    14881 ggcggcccgg cggcaacgcc aatccgctgc agaagctact catgcgcctc attgcgcgac
    14941 aacttgcgcg gcgcggctga aggcaacaca tcatgaacac gaccggcatg ctgacgcagg
    15001 acgcgatacg cgacattctc gaagcgggcg tctgggcacc ctcggcggaa aaccgccatg
    15061 ccttccgcta cagcatcgaa ggcgacgacg tacgcctgca tgtggacgcc gcattcaagc
    15121 aaggaagcgc cgatcaacag tacgtgacgc tgctgtcact gggtgcagtg atcgagaaca
    15181 tgcggatcgc cgcgagcgtg catgcgctct cgtgcacggt gcgccgtgaa ggcgaggcga
    15241 tcgatgccga catcgtgctg cacttcacgc cggcctcgct gcctgcggac gaactggccg
    15301 ccgcactcaa gcagcgccac accaatcgcc ggcccttcta tcacggaccg cgcctgagcg
    15361 ccgccgaaca aggcgagatt gaggcaggca tcgtgccgct gggcgaccaa tggctggtgc
    15421 gctggttcga cgaagaacac ggccgtgccc ggcttgcgcg tctgatgcgc gaagccgaga
    15481 cgcaccgctt cgccaacgcc gaactgcatc aggcgatgtt cggcggcatc cgcttcgatg
    15541 tgggttggag cgcgggcgcc aatgaggggc ttccgcccgc ggcactgcat atcgagccgg
    15601 gcacgcgcgc cgccttcgcc gcgctgcgcc gctggtcctt catgcgcacg ctcgtgcgcg
    15661 tcggcgcgca tcatgcgatc gccatgcgcg ccagttacat ccccgcgcgc cggtcgccgc
    15721 atctatgcgt cgtcgcctcg cgtacaccgg tgcacgatgc cgacgagctg ctgcaagccg
    15781 gttgcgccat ggagcgtgta tggctggcgg caacgcggct caaccttgcg gtacagccga
    15841 tggctgccgc cgtcgcgctg gcccgaccgg gccgcagtgc gttgactgcg caacagcgcg
    15901 agtcgctcgt tgccggctgg cgcgcgctgc tgcccgacgc acaacccgta atgatcttcc
    15961 gcttcggtcg tgccgcaccg cctgccgtgc gcgccggtcg ccccttgcct tcctcgctcg
    16021 cacggcgctg acgcgccacc gccgctgcaa caatttttca ctactgcgcg catcgcgaag
    16081 cggccgtacg gcgtgcgtgg acgacgtctc atcaagcgag tcgtttgact ttcaatcgac
    16141 atcatcgcgc aagacaaagt atgcgtctct gtacgcattg cgaatcggct ttgttctttt
    16201 cgttccgttc cgtcaacaac tgtcgcgtgc gcacgttccg gcgcagtcat tgcctcgttc
    16261 tgcgcgaatg aaacaggtcg gaaagagcag ttaaacattg tcaactgcgc gcatggggat
    16321 tgggcgcggc gttagtctcg cggctctgct tcactgtctt gcaccgcgtt tcaagcgcgc
    16381 gtgccgcgcc cgatgtcaaa ggacttgagc ccgattcggc ccgtctcgaa tacaccaatg
    16441 aacaattcgc acaaagcgta caagggaggc gtttgatgtc cgcagcactc cgattcattt
    16501 atcgccgtca gctcgcattg ctggcgctct tcggcgccgc gctgctggcc ggttgcggca
    16561 ccggcatcgg ttatccgccc gcgcccaccg tcgcggcaac gcccgactat cgctacgtgg
    16621 tcggtccggg cgatcaggtc agcatcatcg tgtggcgcaa ccccgagctc tcgctcgcgg
    16681 tgccggtgcg ccccgacggt ctgatcacca cgccgctggt cgaagacttg caggcggcgg
    16741 gcaaggatcc gaccacgctt gcgcgcgaca tcgaacaggc gctgagcaaa tacattcgcg
    16801 atcccatcgt cacggtgatc gtcaccaact tcgtcgggcc gtacagcgag caggtgcgcg
    16861 tgatcggcga agcgggcaag ccgcagacgc tgccctaccg gcagaacatg accgtactcg
    16921 acgtgatgat cgccgtgggc gggctgaccg atttcgccga cggcaacagc gcggtgctgc
    16981 tgcgcacgtc ggagggcggc aagcagtaca ccgtccgctt gaaggatctc gtcaaacgag
    17041 gcgacgtcac cgccaacgcg gacgtcaagc cgggcgacgt actgatcatt ccgcaaagct
    17101 ggttctgacg ggatggagga ggctggagcg accggagact acgtgcggcg gacggtgacg
    17161 gcgcgagcgg tgagcgccgc ccgtccacgc gcgaacgaca acgatccgga acgctttggc
    17221 gtcgagctgt agcaagtacc gcgtttttcg aggtgtgatt catggacgag ttgctgcaac
    17281 aactgatgac ctacctgcgc ggcatgtggc gccgacgttg gccgggcctg gccgtggcgt
    17341 ggatcgtcgg catcgccggg gccgtcatgc tgttcaagat gcccgaccag tacgaggcga
    17401 gcgcgcgggt gtacgtcgat acgcagtcgg tactgaagcc gctgatgtcg gggctcgcgg
    17461 tgcagccgaa cgtcaatcag cagatcgcca ttctgagccg cacgctgatc agccgaccga
    17521 acatggagaa gctgatccgc atggccgatc tggatcacaa catccgaacc aatcgccaga
    17581 aggaacagct gatcgacagc ctgatgaaga agctggaact gcgcggcagc gatcggcaga
    17641 acctctacac catcaactat cgcgatacca atccggccga ggcgcggcgg gtggtgcagt
    17701 cgctgctgtc gatgttcgtg gaatcgggca tcggcgacaa gcgcaagggt tcggataccg
    17761 cgcggcgctt catcgacgac cagatcgccg tgtatgaggg caagttgcgc gaggccgaga
    17821 accgcgtgaa ggaattcaag ctcaagcaca tgggcatgat gggcggcgac ggcaaccagt
    17881 actacgaaca gatgcagcgc ctcaacgagg aactcgcgcg ggtgaatctg gaactgcgcg
    17941 ctgccgagca ggtgcgtgat tcctaccgca gccagctcgc cggcgaagaa gcggtgtaca
    18001 tgccggacgg cacggcactc gttgcgcgcg ggccgatgtc cgaaatcgac atgcgcatcg
    18061 aggcgcagaa acgcagcctc gatgatctgc tgcgccgttt taccgaggcg catccggatg
    18121 tgcagtacac gcgccgcatg atcgcttcgc tggaagcgga gaagaaggag cggggcactt
    18181 cggacaccgc catgagcggc aacgtgggcg gcatgggacc gatcaacact aacccggtgt
    18241 tccagcaact caaggtcgcc ctggccgaat ccgaagcgaa cgtcgccgcg ctgcaggcac
    18301 gaacgcagga attggcgtct cgcgtggaga agatgcgtgc ttcggcgcag atgctgcccg
    18361 aagtggaggc gcagtacgcc gcgctcaatc gcgattacga cgtgcagcga cgcaattaca
    18421 cgacgctggt gcagcgccgc gagtccgcca cgctgtcggg cgaaatggat gcggcggccg
    18481 gcctggccga ctttcgcatc atcgatccgc cgtcggtgtc ggcgacgccg gtggcgccga
    18541 accggctgct gatgctgccg gccgtgctgt tcgtcgcgct cggcgccggc ctgtttttcg
    18601 ccttcgtcat cagtcaggca ctgcccacct tccacgatgc gcgcggcttg cgcgaagtgt
    18661 cgggccgacc ggtgctcggt acggtgtcgt tattgaccaa tgcagcgatg aaccgccagc
    18721 ggcgccgcaa cattgcgctg ttcgcgggtg gactggccgg cctgttcgcc gtctacggtg
    18781 cggcgctcat gctgctcacc gtcggcggcc cccgaatctg agaggtgtgt catgagtctc
    18841 atcgaaaaag ccgccaagcg gctggaagaa ctgaagcgca ccggcgtggc ggtatccgaa
    18901 gaggaactgg cggcagcggc gggtgcgccg gtcgagcatt acgacgtcag cgccgagtac
    18961 acaccgctca agcagcagat gcagcccgag gaacccgccg gtccggtgca gtacagccgc
    19021 atcgtcgata tcgatctgtc gcgccttgcg gccatgggca tggtcacgcc cgaagcgccg
    19081 cattcgccca tcgccaacga gtttcgcgta gtgaagcgcc cgttgctggc gaacgcacag
    19141 ggcaaatccg ccgtgccggt gaagaacggc aatctgatca tggcgaccag cgcgctgccc
    19201 ggcgaaggca agagcttcag cgccctcaac ctcgccatga gcatggcgat ggaactggac
    19261 agcacggtgc tgctggtgga cgcggacgtc gccaatccga gcctgctcaa ggtgctcggc
    19321 ctgccgccgg cgcgcggcct gatggacgtg ctgaccaacg acgacgtgag cctgagcgac
    19381 gtcatcctgc gcaccaacgt ggagaagctg tccatcctgc cggccggcac gcatcaccag
    19441 cgggcgaccg aagtcctggc cagcgacgtg atggcgcaac tggtggccga aatggcggcg
    19501 cgctatcccg accgcatcat cattttcgat tcgccgccgc tgctgcccac gaccgaatcg
    19561 cgcgtgctcg cgacgcacat gggacaggtg atcgtcatcg tcgaggcgga catcaccacg
    19621 cacagcaacc tcaagcaggc gctcgcgcac gtggagaact gcccggtcgt gctcaccatg
    19681 ctgaacaagg ccgtccggtc ggaggtcgcg cagtactacg ggtattacgg ctattcgcgc
    19741 gccgcacgcg cgtagcgggc gtgacgcggc gcgcacggct ccatgcgccg cgtccggtcc
    19801 ggacgcacca atatcaataa cgatcaggga tcttgtggca caaaaactcg cccatcgtgg
    19861 agcgggcgcg gccgacgctc gacgcaagca ggcggtcatc gcactggcgg tggcttcgat
    19921 gttcgtgatt cctgccgcgc aggcggcgaa atggacgatc acgccttcca ttcgcgtgca
    19981 ggaaacatgg accaacaacg tgtcgttgtc cgaagacggc gaagaggatt gggacttgat
    20041 caccgaagtc gaaccgggcc tgtacgcccg cggccaaggc gaccgcctga acttcgtgct
    20101 cgactaccgc gcactgggcc tgctttatct gcaggacagc gaccgcaacc gcgtcgatca
    20161 tcgcttcaac ggcaagctca cttacgaggc gatcaacgac tttgcctata tcgatgtggg
    20221 cggcacggcg acccagcagt tcgaatcggc tttcggcgcc atgccgatcg acgacagcag
    20281 cgacaccgat aaccgctaca ccgcgtcctc ctattacgtc gctccttatc tgcgcggacg
    20341 actattcggc gattcgacct atctgctgcg ctacagcagc atctggacca acgccggcca
    20401 gagctcggtg ctggaggacg ggcataccaa ccagtacacc ggacgcttca acacgccgat
    20461 ccacacgttc gggctggcgc tgacctacga ccgcaacgat accgagtacg acggcaactc
    20521 cgacgaattg agcaccgaga tcgcgcgggc gatcggctac tggcgcatcg cgccgcaatt
    20581 catcctgcag gcgcgcggcg gctatgagga gacggagtcg ctgttcacca atcgatcgga
    20641 ggaaatctac ggcggcggtt ttctttggta ccccacgccg cgcacgtcgg tagaggcttt
    20701 tgccgagcat cgcgtattcg gcaccggcta tttgttcgac gcggcgcatc ggcgccgctt
    20761 cgcgacattc cgtgtccgcg ccagcaagga tctgacgact tactcgcaga acctgcatcc
    20821 cgccgtgctg gtggaattgc tgatgaacga tccgcagttc atcgaacagg cgcagaacga
    20881 tcctgtgctg ctggagcagc tcgcgcgtca gcggttgcgc gagctcggcc tgcgcgacac
    20941 gcagcaggcg ttctacagcc agcagatcca gatccaggaa cggcaggagg cttcggtctc
    21001 gctgacgggc atacgcaaca acgtgatctt caccgtctat cgcctggata gcgaaaactt
    21061 cgccgacgag gacaacccgt ccgttgcgct gctggatgcg cgcttcggcc ggcatatcga
    21121 acagcgtggc gcgtcgatca gctggacgca ccggctcacc ggccgcacgc gcacgaacgt
    21181 cagcctctat cgcactcacg tcaaagagga agagcccttc aaccgcaaat cgaccgcgca
    21241 cacgctgcgc acgagcatca acacggcatt ggggccgaac accaacgcct acgtcggcgc
    21301 gcgctatgtg attttcgatt ccgacgaagg cgcctttcac gactacaagg aagcggctgt
    21361 gttcgtcggt ttcagccatc gcttctagcc cgggctacag caaggtttca tcatgtacga
    21421 atcgttttac ggactcagcg gcaaaccctt ccagctcaat cccgacccgg ccttctacta
    21481 cgccagtcgc ggtcacaagc gcgccttcgc ctatctgcag tacggcctgt tccagggcga
    21541 gggcttcatc gtcatcaccg gcgatgtggg cgcgggcaag accacgctgc tgcgcggcct
    21601 gctcgaacag ctagacagca gcagggtcgt ggcggcacaa ctggtcagca cgcatctgga
    21661 cgccgacgac atgctgaagg cggtcgccgc cgcgttcggc ctgccgatca aatcgacgga
    21721 caaggcggcg ctgctggtgc aactggaaag ctacttcgtc tctcttgccg ccaagcgcaa
    21781 gcgcgctttg ctgatcgtcg acgaagcgca gaacctcacg ccgcgcgcgg tggaggagtt
    21841 gcgcatgctg tcgaacttcc agctcgacaa ccacgcgctg ctgcaaagct ttctcatcgg
    21901 tcagcccgaa ttgcgcgaaa ccatgcgtag tccgcacatg cagcagctcc ggcagcgcgt
    21961 gctcgcttct tatcatctgg gcccgctcga tgcggacgag acacgcgcct atatcgaaca
    22021 ccgcctgacg cgcgtcggct ggacgggcga tccgagtttc gacgacgaag cctttgccga
    22081 aatccaccgc gcatccaaag gcattccccg gcgcatcaac acgctcagca accggctgct
    22141 gatgtccggc tatctgtccg aacgccatcg catcgaaggt ggggacgtgc tcaccgtcgc
    22201 cgaggagatc aaggacgaac tgggcgtgga attgtcgccg gccagcgatg tcggcgatac
    22261 gcgcgccacg ccgcccgccg caccgcaggg cggcttcccg cgcgtgccgc aggcgagcga
    22321 gttcaagggc aacggcttca agctcgatgc cgagcaggcc ggcattgccc ggcagatcct
    22381 gagcggcagc gtgctctccg ggctggaggg ccgcatcacc aatctggagc agacgctgca
    22441 cgccacgctg accctgttgc agcaactcgc gcacgagcac ggcgaaccgg cggagcgcac
    22501 cgcggagcgg cgctgacatg ggcgatctcg gacgctatcc gggcccgatc gtctgcgtcg
    22561 tcggcgcccg gccgaattat atgaaggccg cgccgatcct gcgcgccttc accgcgcaac
    22621 cggcgctgcc gcgcgtcatg ctggttcata ccggccagca ttacgacatc gcgatgaacg
    22681 agcggctgtt cgaggatttg caactgcccc gacccgatct caatctggaa gtcggttccg
    22741 gtacgcatgc agtgcagacc gccgaagtga tgcgcggctt cgagcccgtg ctggacgaac
    22801 tgcggccgag ctgcgtggtg gtggtgggcg acgtcaattc cacgctcgcc tgcgcgctgg
    22861 tcgcggccaa gaagcgcgtg ccggtggtgc atgtggaagc cgggctacgc agcggggacc
    22921 ggcgcatgcc ggaggagatc aatcgcgtgc tcaccgatca gatcgccgat ctgctctata
    22981 ccaccgagcg cagcgcccat gccaatctcg cggccgaagg tgtcggcgcg catcgcgtgc
    23041 acttcgtcgg caacgtgatg atcgattcgc tcctcgcgca tcggcggcgc gcggtgccgc
    23101 cgagcgagac gctgatgcgc gcgggcgcgc cgctatcggt gctcgatcac ccggcgggct
    23161 acggcgtggt gacgctgcat cggccgtcca atgtcgacga gccggaagcg ctggcggaga
    23221 cgttgcagat cctgcgcgaa gtcagcgcgt cgctgccgct cgtctgtgcg ctgcatccgc
    23281 gaacacgcgc caatatcgaa cgcttcgatc tcggacaatt gatcgaaggc gaggccttcg
    23341 tcgtgctgcc gccgcagggc tatctcgaaa tgctcggcct gcaagccagc gcgacggtgg
    23401 tgctcaccga ctccggcggc atgcaggagg agacgaccgc cctgtcggtg ccttgcctca
    23461 cgctgcgcga aaacaccgaa cggccggtca ccatcgagca gggcaccaac acgctggtgg
    23521 gacgcgaccg ccggctgatc ctcgacacgg tggcgagcat cctgcgtgac ggcggcaagc
    23581 gcggccgcac gccggaactg tgggacggcc gtgcggccga gcgcatcgcc gcgcatctgg
    23641 cgcaatggct gcagcgtcgc cccgtgcgcg ccggagcatg agccatgcaa accgcgcgcg
    23701 ccgaggattt cgtcaacgcc ctcaccgtcg acgtggaaga ctacttccag gtgtctgcgc
    23761 tggcgcccta cattccgcgc agcgaatggg gcgccgtcga ttgtcgcgtc gagcgcaatg
    23821 tcgaacgcat cctgcaactg ctgtgcgaaa acggcgcgcg cgcgaccttc ttcaccctcg
    23881 gctgggtggc cgagcgctat cccagcctgg tccgcgccat cgtcgaggaa ggtcatgaat
    23941 tggccagtca cggctatggc caccatcgcg cctccgacca gacgcccgaa gcctttcgcg
    24001 ccgacatcca gctcgccaag atcgtgctgg aggacgccgc cggcgtcgaa gtgaaaggct
    24061 atcgcgcacc gagcttctcc atcggcgagc gcaacctgtg ggccttcgat tgcatcgccg
    24121 aagcgggcta tcgctatagc tccagcgtct atcccgtgca tcacgatcac tacggcatgc
    24181 ccgatgcgcc gcggtttccc tacgaggcac gtgtcggcct gctggaagtg ccgatcacca
    24241 cggtgcggat gttcaacgcc aacctgcccg gcggcggcgg cggttatttc cgcctcatgc
    24301 cttacggcct gtcgcgctgg ctgatccgcc gggtcaattt cgaagaaggg cgttcggcga
    24361 tcttctattt ccatccctgg gagatcgacc ccgaacagcc gcgcgtcaag ggcgtgaagt
    24421 tcaaggcgcg ctttcgtcat tacgtcaatc tgcgccgtac cgagaaacgg ctgcgccgtc
    24481 tgctgcgcga cttcaactgg gatcgcgtgg acaaggtctt cctgggagcg caggcatgaa
    24541 gccggcagat gcgcgcatgg aacaggaaca gatcatggtg cgcgcctgca cgccggacga
    24601 cgccgaacgc tgggaaagct tcgtcgagcg ctgtcccaac gcaagcttct ttcatcgtct
    24661 cggctggaag acggtgctgg aagaggtgtt ccgccatcgc acccactatc tgctggccga
    24721 gcgcgacggc ggtgtggtgg gcgtattgcc gctcgctgaa gtgaagtccg cattgttcgg
    24781 tcacgcgctc acgtcgctgc cctttgcggt gtacggcggc gtagccgcga ccgatgcgca
    24841 ggccgtgagc gcattgcatc gacgcgcgcg cgacatcggc gccgaactgg gggtgcagca
    24901 tctggaactg cgcaaccgag aggtgcgcga agcggactgg ccgcggcagg atctctacgt
    24961 caccttccgc aaggacatcc tgcccgatgt cgagacgaac ctgctcgcca ttccgcgcaa
    25021 gcaacgcgcc atggtgcgca agggcatcgg gaacgggctc gcgagccaca tcgatgcgga
    25081 caccgggcgc ttcttctcgc tctatgccga caacgtgcgg cggcacggca cgcctgcgct
    25141 gccgcgccgt tacttcgaga cgctccgcac cgtgttcggc gacgactgcg aagtgctgac
    25201 ggtgacggac acgcagggca cgccgctgtc cagcgtgttg agcttctact tccgcgacga
    25261 agttctgccc tactacgcgg gcgaccatgt gcgtgcgcgc gagctcgccg ccaacgactt
    25321 caagtactgg gaactgctgc gccgcgcctg ccagcgcggc tacaaggtgt tcgactacgg
    25381 ccgcagcaag cgcggcagcg gctcctatgc cttcaagaag aattggggct tcagcgcgac
    25441 gccgctcgcg tacgaatact gcctgtacaa gcgcgatcgc attccgcaga acaatcccaa
    25501 caaccccaag taccgcgcct tcatagcgct atggcgccgt ctgcccatgc cggtggcgaa
    25561 cgcgctcggg cctttcattg tgcgagggct cggatgaatc gcgcagacga ggccaagcat
    25621 gcacgctgag cggcagatcg aagcgcagtt gtcggtggcg ctgcggccgc ggcagtacgc
    25681 gctgctgggc gtcattgcgt tgtgcctcgt cctcgtgttc gcctggcatg gcgaaacctt
    25741 cgccgccatg gcggccacct ggtggcgcac cgaaaccttc gcccacgggt tgatcgtgct
    25801 gccgatcttc gcctggctgg tgtggcgcga tcgcgcgcgc ctgattgcgc tgcgcatcgc
    25861 gccctatcca ccggcgctgc tcgctctggc cgcggcgggc ttcgtgtgga tgctgggcga
    25921 gctgagcgcc acggtcggcg tctcgcagtt cgccatggtc gccatggtgc cctgcgcgct
    25981 atgcgccctg ctcggtcgcg ccgcgctgcg cgccctggct tttcccctgg cgtttctgtt
    26041 cttcgccgtg cccttcggcg ccttcatgct gccctggctg atgcaactga ccgccgactt
    26101 cactgtcggg gcagtgcgcc tgagcggcgt gccggtctat cgcgaaggca tgctgttcca
    26161 tctgcccagc gggagctggt cggtggtcga agcctgcagc ggcctgcgct atctaatcgc
    26221 ctcggtgatg gtgggtacgc tgtacgccta tctcacctat cgctcgccgc gtcggcggtt
    26281 gctgtttctg ggcgtcgcca ttgccgtgcc gctggtggcc aactggctgc gcgcctactt
    26341 catcgtgatg ctcggtcacc tgagcgacaa ccggctcgcc gccggcgtcg atcacctcat
    26401 ctacggctgg gtgttcttcg gcgtagtgat ggcgatcatg ttctggatcg gcacgcgttg
    26461 gcgcgaggat ctcgacgaca ccgcgcccgc caaaccggcc gagccgcagt tcgacgccgt
    26521 gcgccgcgct ccgcgtcatg cgctcggcgt tgcgctggcc gtgctgctgc tgatcggcgt
    26581 gtggaagccc acggccgcag cgctgagcat gccccaggcc ggccccgacc cggtgctgca
    26641 accgcttgcc ggggtcgcgg cgtggcagcc cgaagcggcg tccttcaccg cgtggacgcc
    26701 gccgcacgac acgccggccg ccacactcga ccaacagttc cgcaacggcg acgcgcaggt
    26761 gggcatgcat gtcgcctact accgcaacca gtcggcggaa tccgaagcgg tgcgcggcga
    26821 aagcgggctg ctcgaaggcc cggacgcggc atggcggcgc atcagcgccc ggcatatcga
    26881 cgtggatgcg ggcgcggacc cgttcagcgt gatgcgcgcc gagttcgtcc atccgctgga
    26941 tcgcatcgtc gcctggcgct ggtactggat cgacggacgg atgacctcga acgcctacgt
    27001 cgccaaggca cagctcgcct gggcgaagct gcgcggcgcg agcgacgact cggccgtcat
    27061 cgtgctctac accccgcgcc gcgacgcaag cgatgacgcc gatcggcgtc tcgccgattt
    27121 tctgcgtgcc atgtcgcccg ctctgttgaa cacgctgcac ggtgcgcggg gaggcgcgtc
    27181 atgaaacgcg atgccgcgcc attcgcggac agccgtccgc tggtcgtgca tgtggtgcat
    27241 cggttcgccg taggcggcct ggaaaacggc gtggccaatc tgatcaacgg catgccggat
    27301 tcgcgtttcc gccatgcggt gctcgccatg accgaagtga ccgacttcaa ggcgcgcatc
    27361 gtgcgcgagg acgtcgccta cttcgaactg cgcaagccgg ccggtcacgc cctgcgttgc
    27421 tatccgcaac tggtgcggat gtttcgcgaa ctgcagccgg cgatcgtgca tacgcgcaac
    27481 ctctcggcgc ttgaggccgc cgtgcccgca atgatcgcgg gcgtgcccgt acgcgtgcac
    27541 agcgaacacg gtcgcgacgt cggcgacctg gacggctcca acgcgaagta ccgctggctg
    27601 cgacgcgcct acagcccctg cgtgcaccgc tatgtcgcgc tgtcccgcga tctggaacgc
    27661 tatctggtcg accgcgtcgg catcgcgccc tatcgcgtgg cgcagatcta caacggtgtg
    27721 gacaccgtgc gcttccgtcc gcgcgaaagc ggacgcgttc cggtggaggg ttcgcccttc
    27781 aacgatccgc gctactgggt ggtgggcacg gtcggacgca tgcatgtggt gaaggaccag
    27841 ctcctgctgg tgcgcgcctt tctgcaaacg cttgcaaccg tacctgaagc gcgtgagcgg
    27901 ttgcgtttga tgctcgtcgg cgacggcccc ttgcgcgccg aagcggaggc gctgctgcgc
    27961 gaggccggcg cgtcgtcgtg cgcgtggctc gccggcgaac gcgccgacgt gcccgaactg
    28021 ctgcgcgcca tggattgctt cgtcctcccg tcgctggccg aaggcatttc caacaccata
    28081 ctcgaagcga tggcgagcgg gttgccggtt atcgccacgc gcgtgggcgg caatcccgaa
    28141 ctggtgatcg atcaggtcac gggcacgcta attccatccg gcgatgtcgc cgcgctcgtc
    28201 gcggcgatca acggctatta cgccttgccg gatgtggcag cggaacacgg tcgggcggga
    28261 cgcgcgcggg tcgagcgcaa gttcagcctc gactacatga cgcaccgcta cgaatctctc
    28321 tacaccagcc tgctcggcga ccgtccggcg acgcgcgcgg gcctgaagac cacttgaggc
    28381 gaaagaacat gtgcggcctg gtcggcatct tcgatacacg cggcaggcgc gacatcgcgc
    28441 ggccggtgct cgaacgcatg aacgaatcgc aacggcatcg cggtccggac gaggacggga
    28501 tccatctgga gccgggcctg ggactggggc atcgccgtct gtcgatcatc gatctgtcgt
    28561 ccggcagaca gccgatctac aacgaggacg gcacggtcgc cgtggtctac aacggcgaga
    28621 tctacaacta tcgcgcgctc atcgccgaac tcacggcact cggccatcgc ttccgcacgc
    28681 gcagcgacag cgaagtgatc gtgcatgcct gggaggagtg gggcgagggc tgcgtcgagc
    28741 gcttccgcgg catgttcgcc ttcgcgctgt gggaccgccg gcgcgaaacg ctgttcctcg
    28801 cgcgcgaccg tctgggcgtg aagccgttgc attacgcgct gcttcccgac ggacaactgt
    28861 tgttcggctc ggaactgaag tccctgctcg cacacggcgg cctggcgcgc gacatcgatc
    28921 cctgcgcggt ggaagagtat ttcgcgctcg gctatgtggc cgagccgcgc agtattttca
    28981 agatggcgca aaaactcccg cctgcgcata cgctgtgcat tcgacgcggc acgccgatcc
    29041 ctgcgccgca cgcctactgg gacgtgcgct tcacgctcga taatcgtata ggcgcagccg
    29101 atgcgcaggc ggagctggaa gcgcgtctgc gcgaatcggt gagcctgcgg ctgatgtccg
    29161 aagtgccgct gggcgccttt ctgtccggtg gcgtggactc cagcgcggtg gtggcgatga
    29221 tggccggcgt gagcgacgag ccggtgaaca cctgctccat cgccttcaac gaaccggctt
    29281 tcgacgaaag cgcgtacgcc agacaggtcg ccgagcgcta ccgcaccaat cactatgtcg
    29341 agcaggtcga cagcgacgac ttcgatctca tcgacacgct cgccgggctg tacgacgagc
    29401 cttatgccga cagttccgcc attccgacct atcgcgtctg ccagttggcg cgcaaacgcg
    29461 tgaccgtggc gctgtcgggc gacggcggcg atgaaagttt cggcggctat cggcgctatc
    29521 gtctgcacat ggccgaagag cgcttgcgcg gcgcgttgcc gctcgccttc cgtcgtccgc
    29581 tgttcggttt gctcggtcgc gtctatccca aggccgattg ggcgccgcgg gtgttgcgcg
    29641 ccaaggccac cttccaggcg ctgtcgcaca atgccgtcga ggcctacttg accagcgtgt
    29701 cgctgctgcg cgatgcgccg cgccgacagc tctacagcgg cagcttccgt gcgcaactgg
    29761 gcgggtatac cgcgcacgag gtgtttcatc gtcatgccgc gcatgccgac gcggacgatc
    29821 cgcttgcgct gatccagtac ctcgacctca agacctatct ggtcggcgac atcaacacca
    29881 aggtggaccg cgccagcatg gcgcattcgc tggaagtgcg cgaaccgctg atggatcacc
    29941 cgctggtgga atggctcgcc accctccctt cgtcattcaa ggtccgcgga caggagggca
    30001 agtacctgct caagaaggcg atggagccgc atctgccaaa agaaattctt tatcggtcca
    30061 aacgcggttt cgccgtgccg ctggcgcgct ggttccgcgg accgctacgc gcgcgcgtac
    30121 ggcaggcggt tctgagcgag cgtctgctcg acaccggctt tttcaatcgc gtgcaacttc
    30181 a
//
LOCUS       DATGOU010000024         9039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_2279182, whole genome shotgun sequence.
ACCESSION   DATGOU010000024 DATGOU010000000
VERSION     DATGOU010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9039
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2279182"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..688)
                     /locus_tag="VKZ48_01485"
     CDS             complement(56..688)
                     /locus_tag="VKZ48_01485"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HLU76557.1"
                     /translation="MASLTEYLRFFVTLTAVVDPFLAIPFFLAFTRGRTAVERADLAR
                     VIALTVFVVLALASFTGESLLRWIGASLPAFRVAGGLVLLLMALAMLNAEAGRVRQTD
                     AEADELQSGDVSGVVPIAFPLLAGPGAISTSIIAVGQGGFVHKLAIVFCVGLVCLVSW
                     VTLAHAHAIASRLSLTALNIATRILGLLLAAMSVQIMAEGLKGLFPALTL"
     gene            complement(850..1101)
                     /locus_tag="VKZ48_01490"
     CDS             complement(850..1101)
                     /locus_tag="VKZ48_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76558.1"
                     /translation="MRNQPFTEPSDELKEIYRQLKLGVAHERVNDENVETLIDMAARN
                     GDTELETLLREWAAPCDDGDDTPSVIAPTPGFNKTNVKH"
     gene            1212..1751
                     /gene="gloA"
                     /locus_tag="VKZ48_01495"
     CDS             1212..1751
                     /gene="gloA"
                     /locus_tag="VKZ48_01495"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HLU76559.1"
                     /translation="MAFTTENHPGVCAEPPAATRGFVLNHSMLRIKDPAVSLDFYTRV
                     LGMRVLRRLDFEEMKFTLYFLACVGPEDNVPQDAGERMVWTFSQRGILELTHNWGTES
                     DAEFKYHDGNAQPQGFGHICISVPDLDAAIRWFDENEVAYVKRPEQGKMKNIAFIKDP
                     DGYWIEILQADRLAELGKN"
     gene            1808..3373
                     /locus_tag="VKZ48_01500"
     CDS             1808..3373
                     /locus_tag="VKZ48_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HLU76560.1"
                     /translation="MSARSFQFDNAFVRELPGDPETDNRRRQVHAAYSRIAPTPVAAP
                     RLIAHSPEVAALLGFDEEWICTPEFAQVFAGNALLPGMETYAANYGGHQFGHWAGQLG
                     DGRAIGLGELVNAAGERWELQLKGAGATPYARSADGRAVLRSSVREFLCSEAMHHLGV
                     PTTRALSLVATGEEVVRDMFYDGHPRPEPGAIVCRVAPSFIRFGSFELPMARGDHALL
                     ERLIDFTIHRDFPHLRDEAPQAARAAWFNEVCERTARLMAEWTRVGFVHGVMNTDNMS
                     ILGLTIDYGPYGWIDNFDRDWTPNTTDAQGRRYRFGHQPQIAQWNLVRLANAIAPVFE
                     SHDALYAGLERYIDVFTETNRRYVAAKLGLVESLDEDMKLMADLHDLLEAAELDMTLF
                     FRALAQIDAAAPNTALFEPAYYDAAKRQTTAAAMQDWVARYAARLRCDNRPSEERRAA
                     MNRVNPLYVLRNYLAQEAIDRAEQGDDGGIRELLDVLRHPYDEQPGRERYAQRRPDWA
                     RNKAGCSMLSCSS"
     gene            3370..4290
                     /locus_tag="VKZ48_01505"
     CDS             3370..4290
                     /locus_tag="VKZ48_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLU76561.1"
                     /translation="MSDGRSAIANPAPASAATGGWIALAPATFVVLWSSGFIGAKFGL
                     PYTGPLTFLTIRFAAVLIVLLPIALALRAAWPSSGRDYVHIAVAGLLLHAGYLSGVFG
                     ALHHGMSAGVIALIVGLQPVLTAMLATAWLGERVTARQITGLVLGLAGVALVVWEKLD
                     LSADAAAFQLALLALCAITFGTLYQKRYCPHLNLLSGATIQYAACLGCMLILAPLLED
                     LHVAWTPQFLFALGWLVFVLSIASIMLLYVLIRHGAATRVTALFYLVPPTTALMAWLL
                     FGERFSVWAMLGMAICAVGVAMVVRQPRPA"
     gene            4370..4678
                     /locus_tag="VKZ48_01510"
     CDS             4370..4678
                     /locus_tag="VKZ48_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76562.1"
                     /translation="MTALLYRYFFFGWLFKDTRRGTLLERAAAWRHNRAQARWLPTYM
                     RRWFCCGALCYGLGAGIELTVAAPFWSALFYVPSALTVSVNAVIAAAYVGLKVMPNPY
                     "
     gene            4765..5736
                     /locus_tag="VKZ48_01515"
     CDS             4765..5736
                     /locus_tag="VKZ48_01515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HLU76563.1"
                     /translation="MSTMSGHADRGREADRPTEIPKRGWKDILLRVKDEIREDNMSII
                     AAGVAFYLLLAIVPAMAAFVAIYGLFADPQTVGTQLDQLGAMLPEDARSMIRQQLTRL
                     ASTSGQALGIGAAVGLLISLWSAMKGTKALMTALNVAYDEREKRGFIRLNLIALLLTA
                     GVIVFVAIALTLIAVIPAVLQLVGLGSVVEWAVQILRWPLILAFLLFVLAALYRWAPA
                     REEARWAWLSPGALFAGALWLLGSVGFSLYVSFFADYNATYGSLGAVVVLLMWMYIST
                     YAVLLGAETNAEIERQTRKDTTTGAPKPMGQRGAYVADTVGESSGNG"
     gene            complement(5694..6242)
                     /locus_tag="VKZ48_01520"
     CDS             complement(5694..6242)
                     /locus_tag="VKZ48_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76564.1"
                     /translation="MRVRQMAVDLGAALAAGYVATRVSDRAQSLLYRMTPVSEKLREP
                     DYPDCSTAEQAARMAADSVGIARTRRRIGVLKSCIHYGLGMGWSAIYPPLRRRGIGPL
                     AAGVATGASLSLLVDEALCPALGITAPNSAYPPTSHARGVATHLVYGLAVAVAAETLH
                     RLLARAGAVQPLPDDSPTVSAT"
     gene            6451..6717
                     /locus_tag="VKZ48_01525"
     CDS             6451..6717
                     /locus_tag="VKZ48_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76565.1"
                     /translation="MMVLGSAPRARESAPVQAWLDAELVLLERLRTILAGATHLDGYA
                     LIPREALDAARELIDSAMGAKSVALDLLHSEMAKDKNCIHKQLG"
     gene            6825..7133
                     /locus_tag="VKZ48_01530"
     CDS             6825..7133
                     /locus_tag="VKZ48_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76566.1"
                     /translation="MERGQEMDEKDDFITLSALADKIVVPGADLRAEVARLLEEDAKL
                     MTRIVQLLERIQPHAQILPSDTALELHSLALLARASRGLKKLLSQRLRYPLQSKAARP
                     "
     gene            7203..7508
                     /locus_tag="VKZ48_01535"
     CDS             7203..7508
                     /locus_tag="VKZ48_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76567.1"
                     /translation="MHIDGYIDALRELREAADRLVDMPGEARSLLSKFLVIDEAMLTL
                     MEVELEASRNVLHALSPSRADRMRAAIQSLAESRSIKGALARRLAQPPGTGDLLPAV"
     gene            complement(7509..7934)
                     /locus_tag="VKZ48_01540"
     CDS             complement(7509..7934)
                     /locus_tag="VKZ48_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HLU76568.1"
                     /translation="MIVYELVCPHQHRFEGWFGSIADFENQRTGGLLSCPVCGDPGVE
                     KLPSARIGGFAAEEAKEGTAVPSAAVSAQDTLAQLLETIMANTDDVGRQFPEEARRIH
                     YEEAPRRAIRGIATVEETRELLDEGIDVLPLPRRREDLN"
     gene            complement(8020..8214)
                     /locus_tag="VKZ48_01545"
     CDS             complement(8020..8214)
                     /locus_tag="VKZ48_01545"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HLU76569.1"
                     /translation="MFCRNLGSVDRIIRVVAGLVLLSLVFVGPATPWGWIGVVPLLTG
                     LIGSCPAYRLFGLNTCATKN"
     gene            8301..8957
                     /locus_tag="VKZ48_01550"
     CDS             8301..8957
                     /locus_tag="VKZ48_01550"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLU76570.1"
                     /translation="MMDPTAPIHPALAQLPADVQQMALRRVQEHELPAGMIVIGHGQP
                     CPGLPLVLEGSIRVQMSDASGNEIVLYRIGADELCTLSVGCLISERPFQAEAVVEAPT
                     RALMLPPKLFDELMQASAPFRRYVLASYGDRLADLMMLVEEVAFRRMDKRLAARLLER
                     APDGVLEATHQVLATELGTAREVVSRLLKEFERNGMVRLERGRIEIVAADRLRQLSEA
                     "
BASE COUNT         1578 a         2943 c         2845 g         1673 t
ORIGIN      
        1 ctttccgacg atgtgctcga ggagacagac ccggagaccg tgggccaact ttccgctaga
       61 gggtcagcgc gggaaagagc cccttcagac cctcggccat gatttgcacg gacatggcag
      121 ccagcagcaa cccgagaata cgggtcgcga tgttgagcgc ggtgaggctc agccgtgacg
      181 cgatggcatg ggcgtgcgcc agcgtgaccc aggacaccag gcacaccagg ccgacacaga
      241 agacgatcgc gagtttatgg acgaaaccgc cttgtccgac cgcgatgatc gaggtgctga
      301 tcgcaccggg cccggcaagc agcggaaagg cgatgggcac gacgccgctc acgtcgccgc
      361 tttgcaactc gtcggcctcg gcatcggtct ggcgtacgcg ccctgcttcg gcgttcagca
      421 tcgccagcgc catgagcaac agcaccaggc cgcccgccac gcgaaaggcc ggaaggctcg
      481 cgccgatcca gcgcagcaac gattcgcccg tgaagctggc aagggccagc accacgaaca
      541 cggtgagtgc gatcaccctc gccagatccg cgcgctccac cgccgtacgg ccgcgcgtaa
      601 aggcgagaaa aaacggaatg gcaaggaaag gatcgacgac ggcggtgagc gtgacgaaaa
      661 aacgcaggta ttcggtgagg cttgccatct cgtctcagcg cgccaagacc gggcatgacc
      721 gcccgcctct gttcacccac gtttccgatt gcatgcccag cttccctgac cgaccctgtt
      781 atgcggctat cttaaccggg tggcgcgatg cccgcagcat gacgactgcc gggccggcgg
      841 gcgatgcgct tagtgcttga cgtttgtctt gttgaagccg ggcgtgggcg cgatgacgct
      901 tggcgtgtca tcgccgtcgt cgcagggcgc tgcccactcg cgcagcagcg tctccagctc
      961 ggtgtcgccg ttgcgcgcgg ccatgtcgat cagcgtctcg acgttttcgt cgttgacacg
     1021 ctcgtgcgct acgccgagtt tcaactggcg atagatttcc ttcagttcgt cggagggttc
     1081 ggtgaacggt tggttgcgca tgacgggtcc ttgtcgtttc ggtcgcagaa ccagggttcg
     1141 caaccgctat gcccgttccg tgcatggcga tttcggtaac atgatgcttt cccttttcac
     1201 cggatttcgt catggccttt acgaccgaaa accatcccgg cgtctgcgcc gaaccgccgg
     1261 ccgccacgcg cggcttcgtg ctcaaccaca gcatgttgcg catcaaggat cctgccgtgt
     1321 cgctcgattt ctacacccgc gtgctgggca tgcgtgtgct gcgccggctc gatttcgagg
     1381 aaatgaagtt cacgctctac ttcctcgcct gcgtcgggcc ggaagacaac gtgccgcagg
     1441 acgccggcga gcgcatggtc tggaccttca gccagcgcgg cattctggaa ctgacccata
     1501 actggggcac ggaatccgat gcggaattca agtatcacga cggcaatgcg cagccgcagg
     1561 gcttcggcca catctgcatt tcggtgcccg atctggatgc tgcgatccgc tggttcgacg
     1621 aaaacgaagt tgcctacgtg aagcggcccg aacagggcaa gatgaagaac atcgctttca
     1681 tcaaggatcc ggacggctac tggatcgaaa tcctgcaagc cgatcgcctt gccgaactcg
     1741 gtaagaattg agtcgagcgt tcaatctcac acaggcggta tgcgcatctg cgccccgccc
     1801 cacccacatg tctgcccggt cattccaatt cgataacgcc ttcgttcgtg agttgcccgg
     1861 cgatccggaa accgacaatc gtcgtcgtca ggttcatgcg gcctactcgc gcatcgcacc
     1921 tacgccggtc gctgcgccgc gcctgattgc ccattcgccc gaagtcgccg ccctgctcgg
     1981 cttcgacgag gaatggatat gcacgccgga attcgcacag gtgtttgcgg gcaacgcctt
     2041 gctgcccggc atggaaacct acgcggcaaa ttacggcggt catcagttcg gtcactgggc
     2101 cggtcaattg ggcgacgggc gtgcgatcgg cctgggcgag ttggtcaatg ccgccggcga
     2161 acgctgggag ctgcagttga aaggcgcggg cgccacgccg tacgcgcgct cggccgacgg
     2221 tcgcgccgtc ctgcgctcgt ctgtgcgcga gtttctctgc agcgaggcga tgcatcatct
     2281 gggcgtgccg acgacgcgcg ccctgtcgct cgtcgccacc ggcgaagagg tcgtgcgcga
     2341 catgttctac gacggccacc ctcgccccga gcccggcgct atcgtctgcc gtgtggcgcc
     2401 ctccttcatc cgcttcggca gcttcgaact gcctatggcc cgaggcgatc acgcgctcct
     2461 cgagaggctc atcgacttca ccattcatcg ggatttcccg catctacgcg atgaggcgcc
     2521 gcaggcggcg cgcgccgcct ggttcaacga agtgtgcgag cgcacggcgc gactgatggc
     2581 cgaatggacg cgcgtcggct tcgtgcatgg cgtgatgaat acggacaaca tgtccatcct
     2641 cggcttgacc atcgattacg gtccctacgg ctggatcgac aatttcgacc gcgactggac
     2701 gccgaacacc accgatgccc agggtcgtcg ttatcgcttc ggccaccagc cgcaaatcgc
     2761 gcaatggaat ctcgtgcggc tggccaatgc gatcgcgccg gtgttcgaat cgcacgatgc
     2821 cttgtacgcc ggcctggagc gctacatcga cgtcttcacc gaaaccaatc ggcggtatgt
     2881 cgcggcgaag ctcgggctgg tcgaaagtct ggacgaggac atgaagctca tggccgacct
     2941 gcacgatctg ctcgaagcgg cggaactgga catgacgctg tttttccgcg cgctggcgca
     3001 aatcgatgcg gcggcgccga atacggcgtt gttcgagccg gcgtattacg acgccgccaa
     3061 acgccaaacg acggccgccg ccatgcagga ctgggttgcg cgctacgccg cgcgactgcg
     3121 ttgcgacaat cgcccgtccg aggagcggcg cgcagcgatg aaccgcgtca acccgcttta
     3181 tgtgctgcgc aactatctcg cacaggaagc gatcgaccgc gccgaacagg gcgacgacgg
     3241 cggcatccgc gaactgctcg acgtgctgcg ccatccttat gatgaacagc cgggccgcga
     3301 gcgctatgcc cagcgccgcc ccgactgggc gcgcaacaag gcaggctgct cgatgctgtc
     3361 ctgcagttca tgagcgacgg ccgatccgct attgcgaatc ccgcgcccgc aagcgccgcg
     3421 accggcggct ggattgcgct cgcgccggcc actttcgtgg tgctgtggag ttccggcttc
     3481 atcggcgcca agttcggcct gccctatacc ggcccgctca ctttcctgac catccgcttc
     3541 gcggcggtgc tcatcgtcct gctccccatt gccctggcat tgcgggccgc gtggccctcg
     3601 agcgggcgcg attacgtcca catcgccgtt gccggtctgc tgttgcatgc gggctatctg
     3661 agcggcgtat tcggtgcgct gcatcacggc atgtcggccg gcgtcattgc gctcatcgtc
     3721 ggcctgcagc ccgtcctgac cgccatgctg gccacagcct ggctgggaga acgcgtcact
     3781 gcgcgccaga taacgggcct cgttctcggt ctcgccggcg tggcgctggt ggtatgggaa
     3841 aagctcgacc tgtcggccga tgcggcggcc tttcaactgg cgctgctcgc actctgcgcg
     3901 atcaccttcg gcacgctcta tcagaagcgt tattgcccgc atctgaatct cctgagcggc
     3961 gcaacgattc aatacgccgc ctgcctgggg tgcatgctca tcctcgcgcc gctgctggag
     4021 gacctgcacg tggcgtggac accgcagttt ctgttcgcgc tcggctggct ggtgttcgtg
     4081 ctgtccatcg cgtccatcat gctgctctac gtcctgatcc ggcacggcgc ggccacacgg
     4141 gtgacggcat tgttctatct cgttccgccc acgaccgcgc tgatggcgtg gctgctgttc
     4201 ggcgaacgct tttccgtgtg ggccatgctc ggcatggcaa tttgcgcggt gggcgtcgcg
     4261 atggtcgtcc gccagccgcg gccggcctga ggccgcgcag gaaccaaagc gcgcctgctc
     4321 gggtctgcct ctcaaacgcg gcttgctctc ctgccgcagt ggaggttgct tgactgccct
     4381 gctgtatcgc tatttcttct tcggctggtt attcaaggac acgcgccgcg gcacgttgct
     4441 ggagcgcgcc gccgcgtggc gccacaatcg cgcgcaggcg cgctggctgc ccacctacat
     4501 gcggcggtgg ttctgttgcg gcgcgctgtg ctacggcctg ggtgcgggca tcgaactcac
     4561 cgttgccgca ccgttctggt ccgccctttt ctacgtaccc agcgccctca ccgtatccgt
     4621 caacgccgtc attgcggcag cctatgtcgg cctgaaagtc atgccgaatc cctactgacg
     4681 agcggcctcg ggtacatcgc gtgcaacgcg gggcaagacg ggcagaacga gcgcccgcat
     4741 ctgtccatgt caacgaggtg cagcatgtcg acaatgagcg ggcacgccga tcgcggtcgc
     4801 gaggccgatc ggccgacgga aattcccaag cgcgggtgga aagacatcct gctgcgcgtg
     4861 aaagacgaga tccgcgaaga caacatgtcg atcatcgcgg cgggcgtggc attctatctg
     4921 ttgctcgcca tcgtgccggc gatggccgct tttgtggcga tctacgggct gttcgcggac
     4981 ccgcaaacgg tcggcacgca attggatcaa ctcggcgcca tgctgcccga ggatgcgcgc
     5041 agcatgatca ggcagcagct cacccggctc gcgtccacgt cgggacaggc gctgggcatc
     5101 ggcgccgcgg tcggcctgct gatttcgcta tggagcgcga tgaaaggcac caaggcgctc
     5161 atgaccgcgc tcaacgtcgc ctacgacgag cgtgagaaac ggggattcat caggctcaat
     5221 ctcattgcgc tgctgctcac cgcgggcgtg atcgtcttcg tcgccatcgc attgaccctg
     5281 atcgccgtca ttcctgccgt tctgcaactg gtcgggctgg gaagcgtggt cgaatgggcg
     5341 gtgcagattc tgcgctggcc gttgattctc gcgttcctgc ttttcgtgct ggcggcgctc
     5401 taccgctggg cgccggcacg cgaggaggcg cgctgggcgt ggctgtcgcc gggcgcgctg
     5461 ttcgccggcg cgctctggct gctcgggtcg gtgggtttct cgctgtacgt cagcttcttc
     5521 gccgactaca acgccaccta cggctcgctg ggagccgtcg tcgtgctgtt gatgtggatg
     5581 tacatcagca cctatgcggt gctgctcggc gcggaaacga atgcggaaat cgagcgccag
     5641 acccgcaagg acaccacgac gggcgcgccg aaaccgatgg ggcagcgcgg tgcctacgtg
     5701 gccgatacgg tgggcgaatc gtccggcaat ggctgaaccg cgccggcgcg cgcaagcagt
     5761 cggtgcagcg tctcggccgc gaccgccacg gccagaccgt acaccagatg tgtcgccacg
     5821 ccgcgggcat gcgaggtcgg cggataggcg ctgttcggcg cggtgatgcc caaggcaggg
     5881 caaagcgcct cgtccacgag cagcgaaaga ctggcgccgg tggccacgcc tgccgcaagc
     5941 ggcccgatgc cgcgccggcg taacggcggg tatatggcgc tccagcccat gcccaggccg
     6001 tagtggatgc aggatttcaa cacgccgatg cggcgcctcg tccgtgcgat ccccacggaa
     6061 tcggccgcca tgcgcgccgc ctgctctgcc gtggaacagt ccgggtagtc cggctcgcgc
     6121 agcttttcgc ttaccggcgt catccgatag agcaggcttt gcgcacggtc cgacacccgt
     6181 gtcgccacgt agcccgccgc cagcgcggcg cccagatcca ccgccatctg cctgacccgc
     6241 atcgttcact ccgtctggat cgacccacgt gatccagcca gtggcatgcc gacgtttcgt
     6301 cggcgattaa gcgaatccgc cctaagctga caagtctcaa tcgaaccgtc ctgaacgcca
     6361 ggcaccatgc ggtcaccgca cgacaccacc atatgaacga tgagcgagaa gagatccaca
     6421 cagcgaagca tccgcctgat ccacgaatcg atgatggtgc tcggcagcgc gccccgggca
     6481 cgcgaatcgg cgccggtgca ggcgtggctc gatgccgagc ttgtcctgct cgaacgcctg
     6541 cgtaccattc tcgccggcgc cacccatctg gacggctatg cgctcattcc gcgcgaggca
     6601 ctggacgcgg ctcgcgaact gatcgattcg gcaatgggcg caaaatccgt cgcccttgat
     6661 ttattgcatt ccgaaatggc gaaagataag aactgcatac acaagcagtt aggctgacaa
     6721 acctcgactt ccgctcacct gcaaccatca gcggattacg cgaaatcttg tgctgcgcca
     6781 ttgatccgta cgccgatctt ctgcacgctg cgcgcgtttc gttcatggag cgggggcagg
     6841 agatggacga gaaggacgac ttcattacgc tatcggcgct ggcagacaag atcgtcgttc
     6901 cgggcgccga tctgcgcgca gaggtcgcgc gcctgctcga ggaagacgcc aagctcatga
     6961 cacgcatcgt gcaactgctt gagcgcattc agccgcatgc gcaaatattg ccctctgaca
     7021 ccgccttgga actgcactcg cttgcgctcc ttgcgcgcgc gagtcgcggt ttgaagaagc
     7081 tgctctccca aaggctgcgc tatccattgc aatccaaagc ggcgaggccg tagtatcccc
     7141 gttgcctcgg cttcgtatgg gccggcttca ctacaacatc aacaaaaccg gtggggacta
     7201 tcttgcatat cgacggatac atcgatgcgc tgcgggagtt gagggaggca gcggatcgac
     7261 tggtggacat gccgggcgag gcccgcagcc tgctgagcaa attcctcgtg atcgacgaag
     7321 cgatgctgac gctgatggaa gtcgaactgg aagcctcgcg caatgtgctg catgcgctgt
     7381 cgccttcgcg ggcggacagg atgcgcgccg ccattcagtc gctcgccgaa tcgcgcagta
     7441 tcaagggcgc actcgcacgt cgccttgccc aaccgccggg tacaggcgac ctgctgccgg
     7501 cagtctgatc agttgaggtc ctcgcgccga cgaggcagcg gcagcacgtc gatgccctcg
     7561 tccagcagct cgcgcgtctc ctccacggtt gcgatgccgc ggatcgcacg cctgggtgct
     7621 tcctcgtagt gaatacgccg cgcttcctcg ggaaactgcc ggccgacgtc gtcggtattc
     7681 gccatgatcg tttcgagcag ttgtgccagc gtgtcttgcg cggatacggc cgccgaggga
     7741 acggccgtcc cctccttcgc ttcctccgcg gcaaagcccc cgatgcgcgc gctgggcagt
     7801 ttttccacgc ccggatcacc gcagaccggg caactcaaca gaccgccggt acgctggttc
     7861 tcgaaatcgg caatcgagcc gaaccagcct tcgaagcggt gttgatgggg gcagacgagt
     7921 tcgtaaacga tcatgaagcg ggtagcgtat aaccgtacag cgacattgca attgtagcgg
     7981 ttatacgccc cgctctcggg ctgtggcgca acgcgccgtt cagttcttgg tcgcgcaggt
     8041 gttcaagccg aacaggcgat acgccgggca cgagccgatc aggccggtca gcagcggtac
     8101 cacgccgatc cagccccacg gcgttgccgg gccgacgaac accaggctca acagcacaag
     8161 gccggccact acacggataa tgcgatcaac agaaccaagg ttgcgacaga acattgaagc
     8221 ctccagcggt tgacgatgcc cgacttatag cgctgccgta gaagcggctc agtgactaaa
     8281 tcaccaagac ccccagcacg atgatggacc cgacagcccc tattcatccc gcactggcgc
     8341 aattgccggc cgatgtgcag cagatggcgt tgcgccgcgt gcaggagcac gaattgccgg
     8401 caggaatgat cgtcatcggg cacgggcagc cctgccccgg cttgccgctg gtgctcgaag
     8461 gcagcatccg cgtacagatg agcgacgcct cgggcaacga gatcgtgctc tatcgcatcg
     8521 gtgccgacga attgtgcacg ctgtcggtcg gctgcctgat ttcggagcgc ccctttcagg
     8581 cggaggccgt ggtcgaagcg cccacgcgcg cactcatgct gccgcccaag cttttcgacg
     8641 aattgatgca agcctccgcg cccttccgcc gctatgtgtt ggcaagctac ggcgatcggc
     8701 tggcggattt gatgatgctg gtcgaggaag tggcgtttcg ccgcatggac aagcgtcttg
     8761 cagcccgcct cctcgaacgc gcgcctgacg gcgtgctcga ggccacccat caggtgcttg
     8821 ccactgaatt gggaacggcg cgtgaagtgg tcagtcggct gctcaaggag ttcgagcgca
     8881 atggcatggt gcgtctggaa cgcgggcgca tcgaaatcgt tgccgcggat cgcttgcgac
     8941 aattgagcga agcctgacct ggtgcccgga gtcggactcg aaccggcatg gccttgcttg
     9001 ggattggcaa gtgattggtg cccggagccg gactcgaac
//
LOCUS       DATGOU010000025         7900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_947973, whole genome shotgun sequence.
ACCESSION   DATGOU010000025 DATGOU010000000
VERSION     DATGOU010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7900
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947973"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..937)
                     /locus_tag="VKZ48_01555"
     CDS             complement(47..937)
                     /locus_tag="VKZ48_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLU76571.1"
                     /translation="MDPSSPLSARAAPAPPVRRGILMLVVAVSFFALLDVTSKWLTQT
                     YPVPMVVWARYSAQTLLMLLLLGPRLGAGLLRTRVPGQQIVRGLVLLGVSVLFLTALS
                     LMPIAEATAISFMAPLILTAMSVVVLRERVGRGAWVAVTTGFIGVLIIVRPGGELFTP
                     VVIVPIVSAFFYATYQLLTRRMAGIDPTMTTLFYGCLVGTVLLSLATPLFWAAPLSVL
                     DGIMLLTTGVLGGLGHFALIRAFELAPASVLAPFLYVQLVIVTGLGYLVFDAFPDVWS
                     LIGMTIIVVSGAWVASRYRR"
     gene            complement(1027..1629)
                     /locus_tag="VKZ48_01560"
     CDS             complement(1027..1629)
                     /locus_tag="VKZ48_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HLU76572.1"
                     /translation="MAEQTNRTHPLLLAAAASVMLFAAVGSAALLGWLPSSHGEQQAT
                     PAPAEPVAQSPAQPVVQTATPKPQPAPAKAVKTVHREPVRIARPALCTSCGTVESVRE
                     VTEAGEGSGLGAVIGGVTGGVVGRQFGGGRGKDVMTVVGVVGGGVAGHQVEKRVRAVK
                     HYEATVRFEDGRTAVYRSDTPAWQVGDRVRLVDGSLRHRA"
     gene            1931..4861
                     /locus_tag="VKZ48_01565"
     CDS             1931..4861
                     /locus_tag="VKZ48_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HLU76573.1"
                     /translation="MKTDALPQPAAASTAHRSSPLYVYLTVLFSLLFLAMGGAIAWVG
                     YSQGRDLTLAATDRVFGYIGKETHASLNQTLVPVGRLADILALQPFVKASSLDGRLQA
                     LPLLQQALVANPQIDAVYAGYPDGSFFMFRALLGEKERSIFFAPALARYLVLSIEAQG
                     KSAQRKQHIFLDKNLQELSRRDIPEYQFDPRTRIWYQSARTSDNQVFTEPYAFYSTRA
                     PGVSLAKHTSSRSVVGIDLTLAHLSERLADLSVLPGMRIVLFDEQGRILARSDTDGAP
                     DHHELGQSLPTTLNASADPLISALRPAVLDEDGAVDMEIGGEEWKIMVQPIETPRGPL
                     YLATAVPLNELLKDLRDTRERHVLISLALLLCGIPLTMLTAHFVTRDIRRVTAQANDI
                     RAFQFDGPSPGRSAIREVDQLAQAMTMTRDTIRNFLDVVTELAEEPKYEPLLRRVVWE
                     TCRAVDAVGGLAYLADPEETLLTPAALMDGNGQPLEKEACEIAASAESPIAAAFRDGQ
                     TRVIDLEKQSCPELSSYQALWPHDSLNALVIPLRQRDGDRVGVLVLFTLKSQTLSAAR
                     LAFAQALSGVAAVVIETRGLIVARKRLLDSFIKLVAGAIDAKSPYTGGHCQRVPELTF
                     MLARAACDAQSGPYKDFQLDDRQWEALQIASWLHDCGKVTTPEYVVDKATKLETIYDR
                     LNEIRMRFEVLKRDARIEILERQLRGEDPATLRAECERRWTELDEEYAFVASCNQGGE
                     TLDAEHIARLERIAQRTWQRTLDDRIGLSADERARKGRTPAVPLPATEYLLADKDEHL
                     IERPAEEELPADNPWGFKLRAPKYKYNRGELHNLRIARGTLTEEERYVVNHHIVQTII
                     MLSQLPFPPHLRNVPEIAGGHHEKMDGTGYPRGLVREEMSDTARMMAIADIYEALTAS
                     DRPYKKAKPLSEALRIMQRMKNEGHIDPQLYELFIDSGVWRDYAGRFLDPEQIDVSDA
                     AAYR"
     gene            5479..6495
                     /locus_tag="VKZ48_01570"
     CDS             5479..6495
                     /locus_tag="VKZ48_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76574.1"
                     /translation="MATLARNLSIAMSKVKLADLAKIPFIDAENLFGRTIATLSFFEK
                     GEWHLWVPGGDAGLIKISGWPAEGYYFGDQAEKDTDAYLDFFNFIAQRCCWPPVVRPF
                     FALQQDYFNLCASIKKFDILFEYSERLRDSTARLVATELEYLFALCRSVFDLLQEMIA
                     AQWDAVQLLDASIKKKQLPRTFSDVALKAGSPRAIDELVQRFSIPPMLAAFYVRSAPF
                     FEILRNFRDRFIHGGTTPEQVFVTERGFAVSRTSQPFCAFDVWTEEHMLPNQLCSLRP
                     VIGHLVLETLKVCEDYAATTQSIIQYPPPIAPDMYFFVRGYFNDALMECSVARDQCLW
                     WNRD"
     gene            complement(6690..7670)
                     /locus_tag="VKZ48_01575"
     CDS             complement(6690..7670)
                     /locus_tag="VKZ48_01575"
                     /inference="COORDINATES: protein motif:HMM:NF019077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1513 domain-containing protein"
                     /protein_id="HLU76575.1"
                     /translation="MLTCWSDDPQRPRSFHAGLQSAPQHALSLPARGHAVQWHPHDRT
                     SAFVVARRPGSFLVHWRLADGKELARFEAEDDLYFEGHITFDASGKRIFATQTDTFTG
                     SGQIGVYDADTLERIDAWPTHGIGPHEVRLLSNGLLAVCNGGIQTLPETGRAKRNLDT
                     MDPSLVLLDPRDGTPLEQYKLPDHRMSIRHIAQAEDGTIGVALQNEGDELLPLFAFLK
                     SGRLNYGEANEQVLTACGTYAGDIAALDGHFAVSCTLGGVTAIWNSRGKAIAQAPTPR
                     VCALAPVTSGWLASADAGMLWQLSADGKMLGASRGEVAWDNHAVALDESA"
BASE COUNT         1531 a         2476 c         2482 g         1411 t
ORIGIN      
        1 ggccgacacg cacgacctct acggcttgcg cgtcaagagt tgaggctcag cggcggtagc
       61 gactcgccac ccatgcgccg ctcaccacga tgatcgtcat cccgatcagc gaccacacgt
      121 ccggaaaggc gtcgaacacc agatagccga ggccggtcac gatcaccagt tgcacgtaaa
      181 gaaagggcgc cagcaccgaa gccggggcca gttcgaaggc acggatcagc gcgaagtgtc
      241 cgagcccgcc cagcacgccg gtggtgagca gcatgatccc gtccaggacc gacagcggcg
      301 ccgcccagaa cagcggcgtt gcgagcgaca gcagcacggt gccgaccaga cagccgtaga
      361 acagcgtggt catggtcgga tcgatgcccg ccatcctgcg cgtcagcaac tgataggtgg
      421 cgtagaagaa cgccgagacg atgggcacga tcaccacggg cgtgaacaac tcgccgcccg
      481 gacgcacgat gatcagcacg ccgatgaatc ccgtcgtcac cgccacccac gcaccgcgcc
      541 cgacccgttc gcgcagcacc accaccgaca tcgcggtgag gatgagcggc gccatgaagc
      601 tgatggccgt cgcctcggcg atcggcatca acgacagtgc ggtgaggaac agcaccgata
      661 cgccgagcag taccagcccg cgcacgatct gctgaccggg tacccgcgtg cgcagcaggc
      721 cggcgcccag acgcgggccg agcagcagca acatcagcag ggtctgcgcc gagtagcgcg
      781 cccacaccac catcggcacg ggataggtct gcgtcagcca cttcgacgtg acgtccagca
      841 gcgcgaagaa gctgaccgcc acgacgagca tgagaatgcc gcggcgcacg ggcggggcgg
      901 gcgcggcgcg ggccgaaagc ggggaggagg gatccatgag gcgtcggccg cattataggc
      961 agcggcgcgg cacaaaaaag ccacggccga atgttcgtgc cgtggcaaac cctcctccaa
     1021 gggtctttat gcccgatgac gcaggctgcc gtccaccaat cggacgcgat cgccgacctg
     1081 ccaggcgggc gtgtcgctgc gataaaccgc cgtgcggccg tcctcgaagc gcacggtcgc
     1141 ttcgtaatgc ttgacggcgc gcacgcgctt ttcgacctgg tggccggcga caccgccgcc
     1201 gaccacgccg acgaccgtca ttacatcctt gccgcgtccg ccgccgaatt gccggccgac
     1261 cacgccgccc gtgacgccgc cgatgacggc gccgaggccg gaaccttcgc cggcctccgt
     1321 cacttcgcgc accgattcga cggtgccgca actggtgcag agcgccggcc gcgcgatgcg
     1381 caccggttcg cgatggaccg ttttcacggc tttcgccggc gcgggctgcg gcttgggcgt
     1441 cgccgtctgc accaccggct gcgcgggtga ctgcgcaacc ggctctgccg gcgccggcgt
     1501 ggcctgctgc tcaccgtgcg acgagggcag ccagccgagc agggcggcgc tgccgaccgc
     1561 ggcgaacagc atcaccgatg ccgcggcggc cagaaggagg ggatgcgtgc ggttcgtttg
     1621 ttcagccatg ttcacacctt cttcgaaacg tgacaggctc attaaggcgc aacgcgcgcc
     1681 gcacgaagcg cgagtttgta acgatgcgtt gcggccgacg gcgcggaaac aattcgtagc
     1741 aggtgagtgc gaaagcctcg cgaacgcggc cgattcggcg cgcgcagcgc ttttaaccgg
     1801 aaagaaccgg gaatctgtcg cggtgcacga gggggactgc gcgcgggtcc gcaaagcgag
     1861 aaatcttgcc tttattcgcc gggcgtgctg atgcacgctc atcgcttcct acgcgcggaa
     1921 cgaagaccga atgaaaaccg acgcgcttcc acagcctgct gccgcctcca ccgcgcaccg
     1981 gtcgtcaccg ctatacgtgt acctgaccgt gctgttctcg ctgctgttcc tggcgatggg
     2041 cggcgcgatc gcgtgggtcg gctactcgca ggggcgcgat ctcacgcttg ccgcgaccga
     2101 tcgcgtgttc ggctacatcg gcaaggaaac gcatgccagc ctcaatcaaa cgctcgtgcc
     2161 ggtcggccgt ctggccgaca tcctggcgct gcagcccttc gtcaaggcat cgagtctgga
     2221 cggcagactg caggcgctgc cgctgcttca acaggcgctg gtcgccaacc cgcagatcga
     2281 tgcggtgtat gccgggtatc ccgacggcag ctttttcatg ttccgcgcat tgctcggcga
     2341 gaaggaacgc tcgatcttct tcgcgcccgc gctcgcacgc tacctggtgc tgagcatcga
     2401 ggcgcagggc aaatccgcgc agcggaagca gcacatcttt ctggacaaga atctgcagga
     2461 actgagccgc cgggacatcc ccgagtatca gttcgatccg cgcacgcgca tttggtatca
     2521 gtcggcacgt acctccgaca accaggtgtt caccgagccc tacgccttct acagcacgcg
     2581 cgcgccgggc gtgtcgctcg ccaagcacac cagttcgcgc agcgtggtcg gcatcgatct
     2641 cacgctggcg catctgtccg agcggctggc ggacctgagc gtgctgccgg gcatgcgcat
     2701 cgtgctgttc gacgagcagg gacgcatcct cgcgcgttcc gacaccgacg gggcgcctga
     2761 ccatcatgag ctgggacagt cgcttcccac gacgctgaac gcgagcgccg atcccctgat
     2821 ctcggcgctg cgcccggcgg tgctcgacga ggacggcgcg gtcgacatgg agatcggcgg
     2881 cgaagagtgg aagatcatgg tgcagccgat cgagacgccg cgcggcccgc tctatctcgc
     2941 cacagccgtg ccgctgaacg aactgctgaa ggacttgcgc gacacgcgcg aacggcatgt
     3001 gctgatctcg ctcgcgctac tgctgtgcgg cattccgctg acgatgctga cggcgcattt
     3061 cgtgacgcgc gacatccgcc gcgtgacggc gcaggccaac gacatccgcg ccttccagtt
     3121 cgacggtccc tcgcccggcc gttccgcgat ccgcgaagtg gatcagctcg cgcaggccat
     3181 gaccatgacg cgcgacacca tccgcaactt cctcgacgtc gtcaccgaac tggcggagga
     3241 gccgaagtac gaaccgctgc tgcgccgcgt ggtctgggaa acctgccgcg ccgtggatgc
     3301 ggtcggcggt ctggcctatc tcgccgatcc ggaagaaacg ctgctcacgc cggccgcact
     3361 gatggacggc aacggacagc cgctggagaa ggaggcgtgc gagatcgccg cgtccgccga
     3421 gtcgcccatc gccgccgcgt tccgcgacgg tcagacgcgc gtcatcgatc tggagaagca
     3481 aagctgtccc gaactgtcgt cgtatcaggc gctgtggccg cacgacagcc tcaacgcact
     3541 cgttattccg ctgcggcagc gcgacggcga tcgcgtcggc gtgctggtgc tgttcaccct
     3601 gaagagccag acgctcagcg cggcgcggct ggcgttcgcc caggccctat ccggtgtggc
     3661 cgccgtggtg atcgagaccc gcggcctgat cgtggcgcgc aagcgcctgc tcgattcgtt
     3721 catcaaactg gtggccggcg ccatcgacgc caaatcgccc tacacaggcg ggcactgcca
     3781 gcgcgtgccg gaactcacct tcatgctggc gcgtgccgcc tgcgacgcgc aaagcggccc
     3841 gtacaaggac ttccagctcg acgaccggca atgggaagcg ctgcagatcg ccagttggct
     3901 gcacgactgc ggcaaggtca ccacgcccga atacgtggtc gacaaggcga ccaagctcga
     3961 aaccatctac gaccgcctga acgagatccg catgcgcttc gaagtgctca agcgcgacgc
     4021 gcgcatcgaa atcctcgagc ggcaactgcg gggcgaggat cccgcaacgc tgcgcgccga
     4081 atgcgaacgc cgctggacgg aactcgacga ggaatacgcc ttcgtcgcga gttgcaacca
     4141 gggcggcgag acgctcgacg ccgaacacat cgcgcgcctg gaacgcattg cccaacggac
     4201 gtggcagcgc acgctcgacg atcgcatcgg tttgtcggcg gatgaaagag cgcgcaaggg
     4261 ccgcacgccc gccgtcccgc tgccggcgac cgaatacctg ctcgccgaca aggacgagca
     4321 tctcatcgaa cgccccgccg aagaggaact gccggcggac aatccctggg gcttcaagct
     4381 gcgcgcaccg aagtacaaat acaaccgcgg cgaactgcac aacctgcgga tcgcgcgggg
     4441 cacgctcacc gaggaagagc gctacgtcgt caaccaccac atcgtgcaga cgatcatcat
     4501 gctgtcgcag ttgccgttcc cgccgcatct gcgcaacgtg cccgagatcg ccggcggcca
     4561 ccacgagaag atggatggca ccggctatcc gcgcggactg gtgcgcgaag agatgagcga
     4621 caccgctcgc atgatggcca tcgccgacat ctacgaagcg ctcaccgcct ccgaccgtcc
     4681 gtacaagaaa gccaagcccc tctccgaagc gctgcgcatc atgcagcgca tgaagaacga
     4741 agggcacatc gacccgcagc tctacgagct gttcattgat tcaggtgtgt ggcgagacta
     4801 cgccggacgc ttcctcgatc cggagcagat cgatgtgagt gatgcggcgg cgtatcgata
     4861 actgtctcgc tggggaaaga ccgtagacat aacttcacgg tggtccgcac ggccttggca
     4921 cgccgtacgc gtgcgtcagg cttaagcagt aacgcgtcct gcggcccctc actagataaa
     4981 gcgacaaaga acgcgcagcg ccaccacgca aaaaagtcgg acgagggagc cttcattgtg
     5041 tgagtgctgc ggtacatggc tatagtctta aactaaaaaa atccgaggtc gcgcgatgag
     5101 ttatacgcgg atgtttgtgc gcggcgaagt cgggtttctt acgtcaggtc cattgagggg
     5161 ttaaaaatga agatacgggt ggcacatcta agagagcgcg ctacaactgg cgggtggatc
     5221 aattttgccg ttttcgacgc taaatccacc aatggcgata acgacggtct tttgtacgaa
     5281 ttaactcagt ccgctcgggc tgctgggctg caaatcgatc aatcggcttt ggcgtttaaa
     5341 gcaggagggc gaattcaatt ctatggagat aagaatcttg tctcgtacct ctctaaagtg
     5401 ggagtgcccc gatggacaca tcaaatagat gtgtgaaacc aaattgttca aggccgccaa
     5461 ccagccggta cgcggtcggt ggcaaccttg gctcgaaact taagcatcgc gatgtcaaag
     5521 gttaagctgg ccgatttagc caagatccca ttcatagacg cggaaaattt gttcggacga
     5581 acaatagcga cactttcgtt ttttgagaaa ggtgaatggc atctatgggt tccagggggc
     5641 gacgccggtt taatcaaaat cagcggatgg cctgcagaag gctactactt cggtgatcaa
     5701 gcagagaaag acacggatgc gtatctcgac ttttttaact ttattgcgca gcgctgctgt
     5761 tggcctcctg tagttcgtcc gttctttgcg ctgcagcagg attacttcaa cctttgcgcg
     5821 tcgataaaga aattcgacat tttgttcgag tattccgaac gactgcggga ctcgaccgca
     5881 cggctggtgg cgaccgaact tgagtatctt ttcgctcttt gtcggagtgt tttcgatttg
     5941 cttcaagaga tgattgcagc gcagtgggat gcggttcagc ttctcgacgc atcgataaag
     6001 aagaagcaac tcccaaggac gttctccgac gttgcgctga aggccggatc gcctcgagcc
     6061 atcgatgaac ttgtacaacg gtttagcatt cccccgatgc tcgctgcttt ttatgttcgc
     6121 tcagcaccgt tctttgagat tcttcggaat tttcgtgatc gatttatcca cgggggcacg
     6181 acgccggagc aggtgtttgt gactgaacga ggctttgccg tttcaagaac gagtcaacca
     6241 ttttgtgctt tcgatgtatg gactgaggaa catatgctcc caaatcagct ttgctcattg
     6301 aggccagtga tcgggcacct cgttcttgaa acgctgaaag tctgcgaaga ttatgcagct
     6361 acgactcagt cgatcatcca gtacccgcca cccatagcgc ccgatatgta cttcttcgtt
     6421 agaggctact tcaatgatgc ccttatggaa tgctctgtgg cgcgtgacca atgcctttgg
     6481 tggaaccgcg actaatttgc accgattcgt ggcaggctgt tttggcggtt ggtgaatgca
     6541 gcagtgaggc tcgaaatgcc cgaacagtta aagagttccc ggcccgtact gttgtcagac
     6601 ttgtggtgaa tatccgtcgc cacgaacgtc gatacccctt ctattttcaa gaagatagat
     6661 gggcgcgaac gcgtcgcatt cgcggacgct taagcactct cgtctagcgc gaccgcgtga
     6721 ttgtcccatg ctacctcacc tcgcgatgca cctagcatct ttccgtccgc gctaagttgc
     6781 cacagcatgc ccgcgtccgc gctggcaagc caaccgctgg tcaccggggc taatgcgcag
     6841 acgcgcggcg tcggcgcttg cgcgatggcc ttgccccggc tgttccagat ggcggtgacg
     6901 ccaccgagcg tgcaactcac ggcgaagtga ccgtccagtg cggcgatgtc gccggcgtag
     6961 gtgccgcatg cggtgagaac ttgctcgttc gcttcgccat agttcagacg gccgctctta
     7021 agaaaggcga acaggggcag tagttcgtcg ccttcgttct gcagggctac accgatggtg
     7081 ccgtcttcgg cctgcgcgat gtggcggatg ctcatgcgat ggtcggggag tttgtattgc
     7141 tccaacggcg tgccgtcgcg cgggtcgagc agcacgagcg acgggtccat ggtgtcgagg
     7201 ttgcgcttcg cgcgtcccgt ctcgggcagg gtctggatgc cgccgttgca gacggcgagc
     7261 aggccgttgc ttagcaggcg gacttcatgc gggccgatgc cgtgggtggg ccaggcgtcg
     7321 atgcgctcca aggtgtcggc gtcgtacacg ccgatctgtc cggaaccggt aaaggtgtcg
     7381 gtttgggtgg cgaagattcg tttgccgctt gcatcgaagg tgatgtgacc ttcgaagtag
     7441 agatcgtctt ccgcctcgaa acgcgccagt tccttgccgt cggcgaggcg ccaatgcacg
     7501 agaaagctgc cggggcggcg cgcgacgacg aaggcgctcg tacggtcgtg cggatgccat
     7561 tggaccgcat ggccgcgcgc gggtaggctc agcgcgtgtt gcggcgcgct ttgcaggccg
     7621 gcgtggaaac tgcgcggacg ttgcggatcg tcgctccagc aggtgagcac ttcgaacttg
     7681 tctgactcag caggtgcgac agcgcgggcg agggcgggcg agagtgcgag ggtcgccgcg
     7741 ctcggcagcg cgcaaagcaa agcgcgtcgt cgtggatcaa tccccatcgc tttctccgaa
     7801 gccgaccgac accttgatgg catcggcgat gtcttccgcc agcagcgcat gcagcttcgc
     7861 cagttcgcgt gcggcggcgc gtgcggcatc cttgtccgcg
//
LOCUS       DATGOU010000026        10232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_2724167, whole genome shotgun sequence.
ACCESSION   DATGOU010000026 DATGOU010000000
VERSION     DATGOU010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10232
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2724167"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VKZ48_01580"
     CDS             complement(<1..479)
                     /locus_tag="VKZ48_01580"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLU76576.1"
                     /translation="MDKRIYLLTSIAFVVGMVELIIGGILDLVALDLGVSIGHAGLLI
                     TAFALVFAVSGPLLLLLVGQADRKRVTLAALLVFIAGNFVAVASTTYGTLMLSRIVTA
                     ASGALLTVLALAMAAHISRPENRGRAIGLVIMGISGSLVLGLPIGVSMGHAYGWRSP"
     gene            850..1053
                     /locus_tag="VKZ48_01585"
     CDS             850..1053
                     /locus_tag="VKZ48_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76577.1"
                     /translation="MFQSPLHYCRQRDIWVALDMSVRQCRAMHACALPCPYEALFAAV
                     YEEHKLVVARCKGRAPEHEAARG"
     gene            complement(1089..1484)
                     /locus_tag="VKZ48_01590"
     CDS             complement(1089..1484)
                     /locus_tag="VKZ48_01590"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HLU76578.1"
                     /translation="MALDATQREELIALIDREMARLRAEVQEGIERSGRTAPYSALTG
                     EAYDSGDQAVADLLTDVEIAEVKRDMSAIEALEAARGRALSGVADTCIDCGGEIPFAR
                     LRANPAARRCRPCQEQYESTHALSAAAQR"
     gene            complement(1589..2209)
                     /locus_tag="VKZ48_01595"
     CDS             complement(1589..2209)
                     /locus_tag="VKZ48_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008349056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HLU76579.1"
                     /translation="MKNFLARYWRILRRPPVHLSFGLIIVASFAAGIIFWGGFNTAME
                     LTNTEPFCIGCHEMRDNVYVELQDTIHFTNRSGVRAKCSDCHVPHDWTDKIARKMQAS
                     KEVWGKIFGSINTREKFLDKRLELASREWERMKANNSLECRNCHGYEYMDFTRQSKRA
                     ADAHSTYLARGTHTCIDCHKGIAHQLPNMAGVPMWSPKSEEAPALQ"
     gene            complement(2206..2700)
                     /locus_tag="VKZ48_01600"
     CDS             complement(2206..2700)
                     /locus_tag="VKZ48_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HLU76580.1"
                     /translation="MNKACLRIVLLACGLLATAAAAQQSGLVDAMRGRTPLAEETRPP
                     VLPNVENKDLRRERAYTMQPPTIPHKIDGYQLDKNANRCLFCHNRDRIPETQAIPLSV
                     THYMDRDGNVRAAISPRRYFCTQCHVTQTETKPLVDNTFQDVDAVLKRTGGAQTKAER
                     TPAR"
     gene            complement(2709..5204)
                     /gene="napA"
                     /locus_tag="VKZ48_01605"
     CDS             complement(2709..5204)
                     /gene="napA"
                     /locus_tag="VKZ48_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HLU76581.1"
                     /translation="MDDARRIFMKQSAAAAVAAAGGIPLPAVSQNLVTDPTQTQLKWS
                     KAPCRFCGVGCGVTVAVKDNRVVATHGDMNAEVNRGINCIKGYFLSKIMYGADRLTTP
                     LLRMKNGRYDKNGDFQPVSWDQAFDVMAAQFKRVLKEKGPDGLGMFGSGQWTLWEGYA
                     GLKLMKAGFRTNNIDPNARHCMASAAFGFMRTFGIDEPMGCYDDIEHADAFVLWGSNM
                     AEMHPILWTRVTDRRLSAPKTQVAVLSVFEHRSFDLADLPIIFHPQTDLAIMNYIAHH
                     IIKTGRVNRDFVNKHTRFMRGNTDIGYGLRPEHQLSVSAKNYENPTGAEPISFEEYAK
                     FVSKYDVNTVAKLSGVPKDRLEKLAELYADPKTKVMSFWTMGFNQHTRGVWCNHLVYN
                     LHLLTGKISTPGNSPFSLTGQPSACGTAREVGTFSHRLPADLLVTNPEHRATAEKIWQ
                     LPAGTIPEKPGAHAVLQNRQLKDGIINAYWVQVNNNMQAAPNMMEEGLPGYRNPNNFI
                     VVSDAYPTVTTLAADLVLPTAMWVEKEGAYGNAERRTQFWHQLVDAPGEARSDLWQIV
                     EFSKRFRMEEVWPKELLDKKPEYRGKTLYDVLFANGTVNKFPLSQIDPEYNNQEAKHF
                     GFYLQKGLFEEYASFGRGHGHDLAPFDTYHQVRGLRWPVVDGKETKWRFKEGSDPYVK
                     AGTGFQFYGNKDDKAIIYALPYEPAAESPDKEYPFWLSTGRVLEHWHSGSMTRRVPEL
                     YRAFPNAVVYMHPEDARALGVRRGSEVRIISRRGEIRSRVETRGRNRMPKGLVFVPWF
                     DASQLINKVTLDATDPISLQTDFKKCAVKIVRA"
     gene            complement(5288..5452)
                     /locus_tag="VKZ48_01610"
     CDS             complement(5288..5452)
                     /locus_tag="VKZ48_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase, NapE protein"
                     /protein_id="HLU76582.1"
                     /translation="MVESERREPTRAQEVGTLLILLAIVAPLLAVMIVGGYGFLVWMY
                     QVFIAGPPGG"
     gene            5528..6223
                     /locus_tag="VKZ48_01615"
     CDS             5528..6223
                     /locus_tag="VKZ48_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HLU76583.1"
                     /translation="MVKVEVAALLAGKASPLGPQGVQSAIAKRPVDGPARIGETGMVG
                     DEQGDRRNHGGPEKALHHYAFDHYARWREELSAPPAILDAPGAFGENISTVGMTEADV
                     CVGDVYAVGSALLQVSQGRQPCYKLNLRFGEKRMARKLQQTGRTGWYYRVLRTGVIHP
                     GDAFELQERPHPQWPLSRLLHVMFIDTLNLDALRAIVALEALSPNWKQTALRRLEKNS
                     VESWARRLGEDVA"
     gene            6360..7034
                     /locus_tag="VKZ48_01620"
     CDS             6360..7034
                     /locus_tag="VKZ48_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLU76584.1"
                     /translation="MPRLKLLSSHDEAGNIRTYVFEAGDTSWIAGQHDTFVLEQAGDD
                     KAVNRRWFTIASAPSEGCIWLTTRISDSPFKQALSRLKPGDAITTQGVGGKFVWEDDA
                     PVPVVLVAGGIGITPFRSILVERKAQGKPLNATLLYFNRDEHIAYRELFDRLAKEHPE
                     FQVRYLINEPITAEKIAELAPLSPQTTVFLSGPKVMVDNVGNALKEKGVPLKQDWFPG
                     YDEGNY"
     gene            7037..7306
                     /locus_tag="VKZ48_01625"
     CDS             7037..7306
                     /locus_tag="VKZ48_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76585.1"
                     /translation="MTDYIKAWQCIGCGRIEATQTCIGVCKDEKVEFVYADEHEAVLA
                     RAKALEALVRRIAWSTPRDDGWERSYRALQAEARRLLAGEQDKAA"
     gene            complement(7311..7994)
                     /locus_tag="VKZ48_01630"
     CDS             complement(7311..7994)
                     /locus_tag="VKZ48_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLU76586.1"
                     /translation="MTQKNIRVLIIDDHTLMRRGLVALLSTEPGIEVSADAADAHEGI
                     KQAGQLQPDVILLDLHMPGISGVQALPALREASPASRVLMLTVSEDSNDLLSALRAGA
                     DGYLLKNIESETLIGAIRRTLDGEAVISERMTHKLVNEVRSAPGGATTEPPASALSPR
                     EQEIVGFIALGASNKEIARELGVAESTVKIHVQHILRKLNLSSRVQVAVYASEHGYAA
                     KAQTRGETA"
     gene            complement(8025..9881)
                     /locus_tag="VKZ48_01635"
     CDS             complement(8025..9881)
                     /locus_tag="VKZ48_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HLU76587.1"
                     /translation="MRTLSARLVAIELVFLLVALLSIGLTLYLSWTLEGSAAAINDAG
                     SLRMRAYHVAALVRDADRDHAAWEAVQLDMVLERLRAGDPARPLALPNTAGVREQLDV
                     LESAWADLKPRLIDHETTVALAEIDGFVLTVDEFVKRIEQANATSTELLRGAQLGLLA
                     LAIIGTVALIYLSFMFVIRPIARLSEGIARMRHGELSVRVPVESRDEFGALTQGFNDM
                     AEALQESYRTLEARVLEKTRDLARQNQQLAMLYDMTAFLSTALTVDDLCRGFVARVVR
                     AYDAVGGTVRLRNTHDGSVHIIASDGVSQDFLAGERCLPDDACACGAAIQQTRSVIHL
                     VRKADARFDLPYCRNERFESVVAVPIDFRHNAIGVFNLFFASPRELAPDERHLLETLG
                     QHLGVAIENLRLIAREREVAVFEERNHLAQQLHDSIAQSLAFLNLQTQMLDDALKQQD
                     PARSTKHLAEIKTGVQECYADVRELLTHFRTRVGDESLEEALHSIFARFERQTGIPVA
                     FSSSGNAHALSPDHELQLIHIAQEALSNIRKHSACTRVEVSLRRGPAYELRVRDDGCG
                     FDPQASRREEHVGLRIMQERSERVGGTLEVRSQPGAGTEILLRVPLAQPQPA"
     gene            complement(9985..>10232)
                     /locus_tag="VKZ48_01640"
     CDS             complement(9985..>10232)
                     /locus_tag="VKZ48_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLU76588.1"
                     /translation="DLIMPGVDGLSLCRALRANPRHGNASIVALTGGEYPEELSGLCD
                     VFLRKPVDVDRLIDTLQQLEASRMLDALPLAGHGLRW"
BASE COUNT         1748 a         3123 c         3418 g         1943 t
ORIGIN      
        1 aaggggctgc gccagccgta agcgtggccc atgctcacgc cgatgggcag accgagcacc
       61 agcgatccgc tgatgcccat gatgacgagt ccgatcgcgc gaccgcgatt ttccggtctg
      121 gagatatggg ctgccatcgc cagggcaagc actgtgagca gtgcgccgct ggctgccgtg
      181 acgatgcgag acagcatcag cgtgccgtac gtcgtgctgg cgacggcaac gaaatttccc
      241 gcgatgaaga ccaggagggc agccaaggtc acgcgttttc gatccgcttg tcccaccaga
      301 agaagcagca gtggtccgct gacggcgaat acaagagcga aagcggtgat cagcaacccc
      361 gcgtgtccga tactgacgcc caagtccagc gcgacgaggt cgagtatccc gccaatgatc
      421 aattccacca tgccgacgac aaacgcgatc gatgtcagca ggtaaatgcg tttatccaag
      481 acgggtatcg ttcctattct ttaaagccgg aggagggccg ggtcgttcat cgatgctcca
      541 gtcggaacat aaaatccgcc gggaaccgat ccaggtttcg agaagttgcg gacttaaggt
      601 cacacattgt gacctctaac aaaggccgtg gcggccgccg tatggcgcct tatgccttca
      661 cggagcaggg tgttgcaatg cttttgtccg tattgaacag tccgcgccat cggcgagcga
      721 tcgcgtagcc ttgcaatgac gtacgcaggt gtgtgcccga accggagtgc cggttgcgcg
      781 tcgtttgact aaagacaaag tgtctcggcg ggattgcgtt agcctgaaga cgcacagacg
      841 gaggtgcgta tgttccaatc cccacttcac tattgtcggc agcgtgatat ctgggtcgcc
      901 ctcgacatga gcgtgcggca atgccgtgcc atgcacgcct gcgcgctgcc ttgcccgtac
      961 gaggccttgt tcgctgccgt gtacgaagag cacaagctgg tcgtcgcccg ctgcaagggg
     1021 cgcgcgcccg aacacgaagc cgcacgcggc tgagcgccgg ccgttgcccg aagggcgtaa
     1081 cgcgcgattc agcgttgcgc cgcggcgctg agagcgtgcg tgctttcgta ctgctcctgg
     1141 caggggcggc aacgccgtgc cgccggattg gcgcgcaggc gcgcaaaggg aatctcgccg
     1201 ccgcagtcga tgcacgtatc ggcaacgccg ctgagggcac ggccgcgtgc cgcttcgagc
     1261 gcttcgatgg cgctcatgtc gcgcttcact tcggcgattt ccacatcggt gagcagatcc
     1321 gcgacggcct gatcgccgct atcgtaggct tcgcccgtca gggcggagta gggcgccgtg
     1381 cggccgctgc gctcaattcc ttcctgcact tcggcgcgca gacgcgccat ttcccggtcg
     1441 atcagcgcga tcaattcctc gcgctgggtg gcatcgaggg ccatgagccg tgctccattg
     1501 tctgggcggt acgactcact ttatgcctcc cgcgccgcgc cggtttgacg aacatcaagt
     1561 ccgggtgacg aagcgggtgc ctgcggcctc actgcagcgc cggcgcctct tccgacttgg
     1621 gcgaccacat gggcacgccc gccatattgg ggagttgatg ggcgatgccc ttgtggcagt
     1681 cgatgcaggt gtgcgtgccc cgcgcgagat acgtcgaatg cgcgtccgca gcgcgtttgc
     1741 tctgccgggt gaagtccatg tactcgtagc cgtggcagtt gcggcactcc agcgagttgt
     1801 tggctttcat ccgctcccat tcgcgcgagg cgagttcgag ccgcttgtcg agaaactttt
     1861 cgcgcgtgtt gatggagccg aagatcttgc cccaaacctc cttcgaggcc tgcatcttgc
     1921 gggcgatctt gtcggtccag tcatgcggga catggcagtc cgagcatttg gcgcgcacgc
     1981 cgctgcggtt ggtgaagtgg atggtgtcct gcaactccac gtagacgttg tcgcgcatct
     2041 cgtgacagcc gatgcagaac ggctcggtgt tggtcagttc catggcggtg ttgaacccgc
     2101 cccagaagat gatgccggcc gcgaaggagg cgacgatgat cagtccgaag ctcagatgga
     2161 cgggcggacg tcgcaggatc cgccaatagc gcgcgaggaa gttcttcatc gtgcgggcgt
     2221 gcgttcggct ttggtctgtg cgccgccggt acgcttgagc accgcatcga cgtcctggaa
     2281 ggtgttatcc accagcggct tcgtctccgt ctgcgtcaca tggcactgcg tacagaaata
     2341 gcgtcgcggc gagatggcgg cgcgcacgtt gccgtcgcgg tccatgtagt gcgtgacgct
     2401 gagcggaatg gcctgggtct cgggaatgcg atcgcggttg tggcagaaca ggcagcggtt
     2461 ggcgttcttg tccaattgat agccgtcgat cttgtgcggg atggtcggcg gctgcatggt
     2521 gtaggcgcgc tcgcgccgca ggtccttgtt ctccacgttc ggcagcaccg gcggacgcgt
     2581 ctcctcggcc agcggcgtcc ttccgcgcat ggcgtcgacc agaccggact gttgtgcggc
     2641 ggcggcggtc gcgagcaacc cgcaggccag cagcacgatg cgcaagcatg ctttgttcat
     2701 ggttcctcct acgccctgac gattttcacc gcgcacttct tgaagtcggt ctgcagcgag
     2761 atcgggtcgg tggcgtccag cgtcaccttg ttgatgagct ggctggcgtc gaaccagggt
     2821 acgaacacca ggcccttggg catgcggttg cggccgcgcg tctcgacacg ggagcggatt
     2881 tcgccgcgcc gcgagatgat gcgcacttcg ctgccgcgcc gcacgccgag cgcgcgcgca
     2941 tcctccggat gcatgtacac cacggcattg gggaaggcgc gatagagctc gggcacgcgc
     3001 cgcgtcatcg aaccggagtg ccagtgttcc agtacgcggc cggtggacag ccagaacgga
     3061 tattccttgt ccggcgattc ggcggccggt tcgtagggca gggcgtagat gatcgccttg
     3121 tcgtccttgt tgccgtagaa ctggaaaccg gtgccggcct tgacgtaggg gtcagaacct
     3181 tccttgaagc gccacttcgt ttccttgccg tccaccacag gccagcgcag gccgcgcacc
     3241 tggtgatagg tgtcgaaagg cgccagatcg tggccgtgtc cgcggccgaa gctcgcgtat
     3301 tcctcgaaca ggcctttctg cagatagaag ccgaagtgtt ttgcttcctg gttgttgtat
     3361 tcggggtcga tctgcgacag cggaaacttg ttgaccgtgc cgttggcgaa cagcacgtcg
     3421 tacagcgtct tgccgcggta ctccggtttc ttgtcgagca actccttggg ccagacttcc
     3481 tccatcctga agcgcttgga gaattcgacg atctgccata ggtcggagcg cgcttcaccg
     3541 ggggcatcga cgagttgatg ccagaactgc gtgcgccgct cggcgttgcc gtacgcgcct
     3601 tccttctcca cccacatcgc ggtgggcagc accagatcgg cggccagcgt ggtgacggtg
     3661 gggtaggcgt cggagacgac gatgaagttg ttcgggttgc gatagccggg cagcccttcc
     3721 tccatcatgt tgggtgcggc ctgcatgttg ttgttcacct gcacccagta cgcgttgatg
     3781 atgccgtcct tcagttgccg gttctgcagg accgcgtgcg cgcccggctt ctccggaatg
     3841 gtgcccgcgg gcagttgcca gatcttttcc gccgtggcgc gatgctcggg gttggtgacc
     3901 aacagatccg ccggcaggcg gtgcgaaaag gtgcccacct cccgcgccgt gccgcaggcc
     3961 gacggttggc cggtgagcga gaaggggctg ttgccgggcg tggaaatctt gccggtaagc
     4021 aggtgcaggt tgtagacgag gtgattgcac cacacgccgc gcgtgtgctg attgaaaccc
     4081 atggtccaga aggacatcac cttggttttc ggatcggcgt acagctcggc gagtttctcc
     4141 agccggtcct tcggcacgcc cgaaagtttg gcgacggtgt tgacatcgta tttcgatacg
     4201 aacttcgcat attcctcgaa gctgatcggc tccgcgcccg tcgggttctc gtagttcttg
     4261 gccgataccg acaactggtg ctcgggccgc aggccgtagc cgatatcggt attgccgcgc
     4321 atgaagcgcg tgtgcttgtt gacgaagtcc ctgttgaccc gaccggtctt gatgatgtga
     4381 tgggcgatgt aattcatgat cgccagatcg gtctgcggat gaaagatgat gggcagatcc
     4441 gccaggtcga aggagcggtg ctcgaagacc gacagcaccg caacttgcgt cttcggcgcg
     4501 gacagacggc ggtcggtgac gcgggtccac aggatcggat gcatctccgc catgttggag
     4561 ccccacagca cgaaggcgtc ggcatgttcg atgtcgtcgt agcagcccat cggctcgtcg
     4621 atgccgaagg tgcgcatgaa gccgaaggcg gccgaagcca tgcagtgccg cgcattggga
     4681 tcgatgttgt tggtgcgaaa gcccgctttc atcagcttga gcccggcgta gccttcccac
     4741 agcgtccatt ggccggagcc gaacatgccc aggccgtcgg gccctttttc cttcagcacg
     4801 cgcttgaatt gggcggccat cacatcgaaa gcctgatccc acgacacggg ctggaagtcg
     4861 ccgttcttgt cgtagcggcc gttcttcatg cgcagcagcg gcgtggtcag gcggtccgcg
     4921 ccgtacatga tcttggaaag aaaatagccc ttgatgcagt tgatgccgcg gttgacctcg
     4981 gcattcatgt caccgtgcgt ggcgaccacg cgattgtctt tcaccgccac ggtgacgccg
     5041 cagcccacgc cgcagaatcg acagggcgcc ttggaccact tgagctgcgt ctgcgtggga
     5101 tcggtgacga gattctgcga aactgcaggg agcgggatgc cgcccgccgc ggcaaccgct
     5161 gcggccgccg actgtttcat gaagattctg cgcgcgtcat ccatggcgat tccccttgtc
     5221 ttcgaatggt ggctcgggtt gcgcggtgtc ctgtgggcgt tcctgaggtc tgtgtcgggc
     5281 gcgcgtatca gccgccgggc gggccggcga tgaacacctg gtacatccat accaggaagc
     5341 cgtagccgcc gacgatcatg actgcgagga ggggggcgac gatggccagc aaaatgagca
     5401 gggtacccac ttcctgtgcc cgtgtcggtt cgcgacgttc ggattcgacc ataatgcgct
     5461 ccgaaaggat gaacgggggg cgtgcaacgt ccatgccgtt cgaatgctac atgagaaggg
     5521 gaggcggatg gtgaaggtcg aagttgctgc gcttttggcc ggaaaagcct cgccgctggg
     5581 cccgcagggc gtacagagcg cgatcgccaa gcgccccgtc gacgggcccg cacggatcgg
     5641 cgagaccggg atggtgggtg acgagcaggg cgaccggcgc aaccacggcg gtccggaaaa
     5701 ggcgctgcac cactacgcct tcgatcacta cgcgcgctgg cgggaggagt tgagcgcgcc
     5761 gccggccata ctcgatgcgc cgggcgcttt cggcgaaaac atttccacgg tgggcatgac
     5821 ggaagccgac gtgtgcgtgg gcgacgtcta tgcggtgggc agtgcgctgc tgcaggtgtc
     5881 gcagggccgc caaccctgct acaaactgaa tctgcgcttc ggcgagaagc ggatggcgcg
     5941 caagctgcag cagaccggcc gcaccggctg gtattaccgc gtgctgcgca ccggcgtcat
     6001 ccatccgggc gatgcgttcg aactgcagga acgcccgcac ccgcagtggc cgctctcgcg
     6061 cctgttgcac gtcatgttca tcgataccct gaatctggat gccttgcgcg caatcgtcgc
     6121 gctcgaagcg ctcagcccga actggaaaca gaccgcgcta cggcggctgg aaaagaacag
     6181 cgtggagagt tgggcgaggc gactgggaga ggacgtcgct taaaaagctt gaaccacagt
     6241 actctgtgtt tcagctttca gcttttcgct tttgagtaaa accccactgc cggcgcgcgt
     6301 ttcgtcatca tggccgccgg agtatgcttg ccccatgccg ccgcagaagg agaataagca
     6361 tgccccggtt gaagttgctg tcttcgcatg atgaagcggg caacatccgc acttacgttt
     6421 tcgaagcggg cgatacaagc tggatcgccg gacagcacga tacctttgtt ctggagcagg
     6481 cgggcgacga caaggcggtc aatcgccggt ggttcaccat tgcctccgca ccgagcgaag
     6541 gctgcatctg gctgaccacg cgcatctccg acagtccctt caagcaggcg ctcagccgcc
     6601 tgaaaccggg cgacgccatc accacgcagg gcgtcggcgg caaattcgtc tgggaagacg
     6661 atgcacccgt gccggtggtg ctggtcgccg gcggcatcgg catcacgccc ttccgcagca
     6721 tcctggtcga gcgcaaggcg cagggcaagc cgctgaacgc cacgctgctt tatttcaacc
     6781 gcgacgagca catcgcctac cgcgagctgt tcgaccggct ggccaaggag catcccgagt
     6841 ttcaggttcg ctatctgatc aacgaaccga ttacggcgga gaagattgcc gaacttgcgc
     6901 cgctgtcgcc gcagaccacg gtgttcctgt cggggccgaa agtgatggtg gacaacgtcg
     6961 gcaatgcgct gaaagagaag ggcgtgccgc tcaagcagga ctggttcccc ggttacgacg
     7021 aaggaaacta ctgatcgtga ccgactacat caaggcgtgg cagtgcatcg gctgcggccg
     7081 gatcgaggcg acgcagacct gcatcggcgt ctgcaaggac gagaaggtgg agttcgtcta
     7141 cgcggacgag catgaagccg tattggcccg tgccaaggcg ctggaagcgc tggtgcgccg
     7201 catcgcctgg agcacgccgc gcgacgacgg atgggagcgc tcctatcgcg cgctgcaggc
     7261 ggaggcgcgg cgtctgctgg ccggcgagca ggacaaggcc gcctgaacct tcaagccgtt
     7321 tcgccgcggg tctgcgcctt cgcggcgtag ccgtgctcgc tggcatacac cgccacctgc
     7381 acccggctgc tgagattgag tttgcgcagg atgtgctgca cgtgaatttt cacggtgctt
     7441 tcggcgacgc cgagttcgcg cgcgatttcc ttgttgctgg cgcccagggc gatgaagccc
     7501 acgatttcct gttcgcgcgg cgacagcgcg ctggccggcg gttccgtcgt cgcgccgccc
     7561 ggtgcgctgc gcacctcgtt caccagcttg tgcgtcatgc gctcagagat caccgcctcg
     7621 ccgtcgagcg tgcgccggat cgcgccgatc agcgtttcgc tttcgatgtt cttcaacaga
     7681 tagccgtccg caccggcgcg caacgcgctg agcagatcgt tgctgtcctc ggacacggtg
     7741 agcatcagca cgcgactggc gggtgacgct tcgcgcagcg ccggcagcgc ctggacgccg
     7801 ctgatgcccg gcatatgcag gtccagcagg atcacgtccg gttgcagttg gcccgcctgc
     7861 ttgatgcctt catgcgcgtc ggcggcatcg gcgctcacct cgatgcccgg ctccgtgctc
     7921 agcagtgcga ccaggccgcg acgcatcagc gtgtggtcgt cgatgatgag cacccggata
     7981 ttcttttggg tcatgtcggc ttcctcttta caagtccggt tcgatcatgc cggctgcggc
     8041 tgggcgaggg gcacacgcag caggatttcg gtgcccgcgc ccggttgcga acgcacttcc
     8101 agcgtgccgc cgacgcgctc gctgcgttcc tgcatgatgc gcagccccac gtgctcctcg
     8161 cggcgcgacg cctgcggatc gaagccgcag ccgtcgtcgc gcacgcgcag ttcatatgcc
     8221 ggcccgcgcc tgaggctcac ttcgacacgg gtgcaggcgg aatgcttgcg gatgttcgac
     8281 agcgcttcct gtgcgatgtg gatgagctgc agttcatggt cgggcgacag tgcgtgtgca
     8341 ttgccgctgc tgctgaaggc gacgggaatg ccggtctgcc gctcgaagcg ggcgaaaatg
     8401 ctgtgcagcg cctcttccag gctttcgtcg ccgacgcgag tgcggaaatg ggtcagcagt
     8461 tcgcgcacgt ccgcatagca ttcctgtacg cccgttttga tctcggcgag gtgtttggtc
     8521 gagcgcgccg gatcctgctg cttgagtgcg tcatcgagca tctgcgtttg cagattgagg
     8581 aaagcgagcg actgcgcgat ggagtcgtgc aactgctgcg ccagatgatt gcgctcttcg
     8641 aacacggcga cttcgcgttc gcgcgcgatc aggcgcaggt tctcgatcgc aacgcccaga
     8701 tgctgtccga gcgtttccag cagatggcgc tcatccggcg ccagttcgcg tggcgaggcg
     8761 aagaacaggt tgaacacgcc gatggcgttg tggcggaagt caatgggtac cgccacgacc
     8821 gattcgaagc gctcgttgcg gcagtagggc agatcgaagc gcgcgtcggc cttgcgcacc
     8881 agatggatca ccgagcgcgt ctgctggatc gcggcgccgc aggcgcaggc gtcgtccggc
     8941 aggcagcgtt cgccggcgag aaaatcctgc gagacgccat cggaggcaat gatgtgcacc
     9001 gagccgtcat gcgtattgcg cagacgtacc gtgccgccga ccgcgtcgta ggcgcgcacc
     9061 acgcgcgcga cgaagccgcg gcatagatcg tccaccgtca gtgcggtgct gagaaaggcg
     9121 gtcatgtcgt agagcatcgc cagttgctgg ttctggcggg cgagatcgcg ggtcttctcc
     9181 agcacgcgcg cctccagcgt gcggtaggac tcctgcagtg cttcggccat gtcgttgaag
     9241 ccctgcgtca gcgcgccgaa ctcgtcgcgc gattccacag gtacgcgcac cgacagctcg
     9301 ccatggcgca ttcgcgcgat gccttcgctc agacgcgcga tcggccggat tacgaacatg
     9361 aaggagagat agatcagcgc gacggtgccg atgatggcca gggcaagcag accgagttgc
     9421 gcgccgcgca gcaattcggt ggaggtggcg ttggcctgtt cgatgcgttt gacaaactca
     9481 tccacagtga gcacgaagcc gtcgatttcg gccagcgcga cggttgtctc gtggtcgatc
     9541 aggcgcggct tgaggtcggc ccacgcgctt tccaggacgt ccagttgttc gcgtacgcct
     9601 gccgtgttgg gcagtgcgag cgggcgcgcc ggatcgccgg cgcgcaggcg ttcgagcacc
     9661 atgtccagtt gcacggcttc ccaggcggcg tgatcacggt cggcgtcgcg gaccagcgcc
     9721 gcaacgtggt aggcgcgcat gcgcaggctg cccgcgtcat tgatcgccgc ggcgctgcct
     9781 tcgagcgtcc acgaaaggta gagcgtgagc ccgatcgaca gcagtgcgac cagcaggaag
     9841 accagttcga tggcgacgag tctggcgctg agtgtgcgca tggcctcatg gcggcggtga
     9901 tccagacgcc atgatgggcc cgcgcggcag cgcgggcttg acctcaatca aggggcgggc
     9961 agggcggcgt ggccctgcgg tcggctacca gcgcaagccg tgaccggcca gcggcagcgc
    10021 gtcgagcatt cggctcgcct caagttgctg gagcgtgtcg atcagccggt cgacgtccac
    10081 cggcttgcgc aggaagacgt cgcacagccc gctcagttcc tcgggatatt cgccgccggt
    10141 gagggcgacg atcgaagcat tcccatggcg cggatttgcg cggagcgccc tgcacagcga
    10201 caggccgtcg acgccgggca tgatgaggtc gg
//
LOCUS       DATGOU010000027         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_4946814, whole genome shotgun sequence.
ACCESSION   DATGOU010000027 DATGOU010000000
VERSION     DATGOU010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4946814"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..>2045
                     /gene="leuS"
                     /locus_tag="VKZ48_01645"
     CDS             158..>2045
                     /gene="leuS"
                     /locus_tag="VKZ48_01645"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HLU76589.1"
                     /translation="MQQSYHPQKIEREAQAHWQKTAAARAVEDAARPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLARFHRMKGYNVLQPMGWDAFGLPAENAAMANGVPPAKWTY
                     DNIAYMKKQLQSLGFAIDWERELATCQPEYYKWNQWLFLRLLEKGIVYKKTGTVNWDP
                     VDQTVLANEQVIDGRGWRTGALVEKRDIPMYYMRITQYADELLDALGTLPGWPERVRT
                     MQANWIGRSEGVEAHFPYAEGEGVLKVFTTRADTLMGATYCAVAAEHPLAAEAAKKDW
                     QLARFVDECKRGSVMEADLATQEKKGMPTGFHVVHPLTGEKLPVWVANYVLMSYGEGA
                     VMAVPAHDERDFEFATQYGLPIRQVIKSKTGAYESVEAPWQDEYAEHGITVNSGEFSG
                     LEFQEAVDAIAAALRNKGLGDKRVQFRLRDWGISRQRYWGTPIPIIHCETCGDVPVPD
                     DQLPVVLPEDCVPDGHGNPLKKREDFLNCTCPKCGKPARRETDTMDTFVDSSWYYLRY
                     ACPDNANAMVDERAQYWLPVDQYIGGIEHAILHLLYSRFWTKAMRDLGLIKLDEPFAN
                     LLTQGMVLNEAFVRVTDSKKRYYWTHELDIQRDGDRVTATSKEDGQPVNYEGWTTMSK
                     SKNNG"
BASE COUNT          415 a          663 c          651 g          316 t
ORIGIN      
        1 ccccgtcggc cctgcgccga cgatcaacac ctgggtacgc acggccccgc tcatacgatc
       61 ccttgtggtc atctggcggc atgatagcgc gcgcgcccgg gcagcatggc agggcggcta
      121 taattcccgg cttccctctg accgccgtcc cattcccatg cagcagtcgt atcacccgca
      181 gaaaatcgaa cgagaagccc aggcgcattg gcagaaaacc gccgcggcgc gcgccgtcga
      241 agatgccgcc cgcccgaaat attactgcct gtcgatgttt ccctacccgt cgggcaagct
      301 gcacatgggc catgtgcgca actacaccat cggcgacgtg ctggcgcgtt ttcaccgcat
      361 gaagggctac aacgtgctgc agcccatggg ttgggacgct ttcggcctgc cggccgagaa
      421 cgccgccatg gcgaacggcg tgccgccggc gaagtggacc tacgacaaca tcgcctacat
      481 gaagaagcag ctccagtcgc tgggcttcgc catcgactgg gagcgcgaac tggccacctg
      541 ccagcccgag tactacaagt ggaaccagtg gctgttcctg cggctgctgg agaagggcat
      601 cgtctacaag aagaccggca cggtgaactg ggacccggtc gatcagacgg tgctcgccaa
      661 cgagcaggtg atcgacgggc gcggctggcg cacgggtgcg ctggtggaaa agcgcgacat
      721 tccgatgtac tacatgcgca tcacccagta cgcggatgaa ctgctcgacg cgctcggcac
      781 cctgcccggc tggccggaac gcgtgcgcac catgcaagcc aactggatcg gccgcagcga
      841 aggcgtggaa gcgcatttcc cctacgccga aggcgagggc gtgctgaagg tcttcaccac
      901 gcgcgccgac acgctgatgg gcgcgaccta ctgcgctgtg gcggcggaac acccgctcgc
      961 cgccgaagcg gcgaaaaaag attggcaact ggcgcgcttc gtcgacgagt gcaagcgcgg
     1021 cagcgtgatg gaagccgacc tcgccacgca ggaaaagaaa ggcatgccga ccggcttcca
     1081 tgtggtgcac ccgctcaccg gcgaaaagct gccggtgtgg gtggcgaact acgtgctgat
     1141 gagctacggc gaaggcgcgg tcatggctgt gccggcgcac gacgagcgcg atttcgaatt
     1201 cgccacgcag tacggtctgc cgatccgtca agtcatcaaa tccaagaccg gcgcctacga
     1261 aagcgtcgag gcgccctggc aggacgaata cgccgagcac ggcatcaccg tgaattcggg
     1321 cgagttttcc ggcctggagt tccaggaggc ggtcgatgcg atcgccgccg cgctccggaa
     1381 caagggtctg ggcgacaagc gcgtgcagtt ccgcctgcgc gactggggta tctcgcgcca
     1441 gcgctactgg ggcacgccga ttcccatcat ccattgcgaa acctgcggcg acgtgccggt
     1501 gccggacgat caattgccgg tcgtgctgcc ggaagactgc gtgcccgacg gtcacggcaa
     1561 tccgctgaag aagcgcgagg atttcctgaa ctgcacgtgc cccaagtgcg gcaagcccgc
     1621 acggcgcgag acggacacca tggacacctt cgtcgattcg tcctggtact acctgcgcta
     1681 cgcctgcccg gataacgcga acgcgatggt ggacgagcgc gcgcaatact ggctgccggt
     1741 cgatcagtac atcggcggca tcgagcacgc catcctgcat ctgctctatt cgcgcttctg
     1801 gaccaaggcc atgcgcgacc tcggactcat caaactcgac gagcccttcg ccaatctgct
     1861 gacgcagggc atggtgctga acgaggcgtt cgtgcgcgtc accgatagca agaagcgcta
     1921 ttactggacg cacgaactcg acatccagcg cgacggcgat cgcgtgacgg cgacgagcaa
     1981 ggaagacgga cagccggtga actacgaggg ctggaccacc atgtccaagt ccaagaacaa
     2041 cggcg
//
LOCUS       DATGOU010000028        13566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_2281707, whole genome shotgun sequence.
ACCESSION   DATGOU010000028 DATGOU010000000
VERSION     DATGOU010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13566
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2281707"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            315..1637
                     /gene="ugpB"
                     /locus_tag="VKZ48_01650"
     CDS             315..1637
                     /gene="ugpB"
                     /locus_tag="VKZ48_01650"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HLU76590.1"
                     /translation="MRFSRLCFGLLFVLSLFAARAQAATEIQFWHSMEGALGEALQTL
                     TDDFNEQQSEYKVTLAYKGSYDEAVGAIRAAFLEGKSPHLVQVYDLSTANLLASKQII
                     RPVYEVLREAGERIDDHAYSPAASSYYSDSRGKLIGLPFNVATPVLYYNRDILKRAGL
                     DPNPSYTTWYDLQEALLKVRHTGATGCALTTTMPAWVLLENTLTWHSQEFATRNNGFD
                     GLDAKLSFNTRLAIRHISLLSSWVKAELFTYAGRRDEGAAMFKRGECAFLTASSSAYA
                     NIDHEAKFDFGVLPMPYYKDINNAPYNTAVGGAGIWVMGGRKPAEYKGVAKFLVYLAK
                     PATQAQWHQATGYLPLSRAAYELTKKTGFYDQRPGTEIPIQTVTHANRPTAYSRGVRL
                     GNYALIRAIIDEELEAVWAQKKSPKAALDTAVKRGNEVLRRFERGSKL"
     gene            1778..2203
                     /locus_tag="VKZ48_01655"
     CDS             1778..2203
                     /locus_tag="VKZ48_01655"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HLU76591.1"
                     /translation="MLNLWPPFRCAGIRVRRISADWRVIDVELRLGLLNRNYMGSHFG
                     GSLFSMTDPFFWLIVMRALGPDYVVSHKGGRIDFFKPGRSHVRAHFEVDDEALDDIRA
                     RTANGERDLPEFSTDIVDENGEVIARATQILHIRRKTAV"
     gene            complement(2200..2769)
                     /locus_tag="VKZ48_01660"
     CDS             complement(2200..2769)
                     /locus_tag="VKZ48_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HLU76592.1"
                     /translation="MLEAFFVSTLAVTIGEIGDKTQLLAFILATRFRKPVPIVLGILV
                     ATLANHAVAGLAGNWIREALEPDTLRWGLGASFLGIAAWAVKPDKLDEAPGTLGCYGV
                     FMCSLVAFFLAEIGDKTQLATIALAAKYEALVAVVGGTTLGMLFANAPVVFLADKAAA
                     KIPFKAIRYSAAVLFAILGGAVLMGFELT"
     regulatory      complement(2792..2881)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            complement(2975..4351)
                     /locus_tag="VKZ48_01665"
     CDS             complement(2975..4351)
                     /locus_tag="VKZ48_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HLU76593.1"
                     /translation="MNQVPQEIFKAYDIRGIVGKTLTPEVVELVGRALGTLCRERGGS
                     AFAVGRDGRLSGPELVQALSKGLRAAGVDVYDVGMVPTPVVYYAAHKLDTGSAVAVTG
                     SHNPPEYNGLKMVVAGVTLSGEEIQALRERIERGDFAQGEGRHIEQDVSADYLDEIVG
                     DVKVARPMSVIVDCGNGVAGSLAPELYRRMGCKVHELYCEVDGTFPNHHPDPSQPDNL
                     KDVIKLLRESDAELGLAFDGDGDRLGVVTKDGEVIYADRQLMLFAKDVLTRVPGGKII
                     YDVKCTTNLAPWIKQHGGEPVLWRTGHSFIKAKLRETGAPLAGEMSGHTFFKERWYGF
                     DDGLYAGARLLEILSRESDASAPLKALPDSVTTPELNIKLREGEPHRMITQLQAGANF
                     PDATQVITIDGLRVEYEDGFGLARASNTTPVIVLRFEAKDQAGLKRIQDDFRSLLLAV
                     KPDVELPF"
     gene            complement(4348..5382)
                     /locus_tag="VKZ48_01670"
     CDS             complement(4348..5382)
                     /locus_tag="VKZ48_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HLU76594.1"
                     /translation="MKQGDLGAQTANGVVLADERAPHASSAYRAPWWLPGGHLQTIYP
                     FLFRRPPRTPTLRERWDTPDGDFVDVDLLEASASAPLLVLFHGLEGGSHSHYARALMQ
                     VARARGWRVLLPHFRGCSGEANRLPRAYHSGDTVELDWMLRRAQAIAGDGPMYAMGVS
                     LGGNVLLKWLGEQGAAANELVRAAAAVSAPLDLMAAGHALERGFNRIYTRMFLATLVA
                     KSMDIIARFPGAFDGERAARARTLREFDDAVTAPLHGFADVTDYWTRASAKPLLRDIR
                     VRTLVMNARNDPFLPAKHLPAHDEVSAAVTLELPVSGGHVGFLSGPFPGHLRWLPQRV
                     FEFFNQADRS"
     gene            complement(5405..5845)
                     /locus_tag="VKZ48_01675"
     CDS             complement(5405..5845)
                     /locus_tag="VKZ48_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLU76595.1"
                     /translation="MQRATKRSGFATAQDAEAAFYEAFERGDLAAMMNVWADDEEVVC
                     IHPGGPRLTGFEQVRKSYAEMFDGRERLRVHLSNQIYMQGMLVAVHSVHEHVLVAGEP
                     KARPPFMATNVYMRTSDGWRMLMHHASAAPVPSELPVEALKRLH"
     gene            complement(5848..6222)
                     /locus_tag="VKZ48_01680"
     CDS             complement(5848..6222)
                     /locus_tag="VKZ48_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HLU76596.1"
                     /translation="MSLGIVLFAHGSRSAEWAAPFTRIRERVAARGKNARVTLAYLEL
                     MQPDFQAAVDELVAQSVERISVVPLFLAAGGHLRNDLPQLVDAALARHPQLRIRVLPP
                     IGESETLLDAIADWAAAESLRE"
     gene            complement(6219..7541)
                     /locus_tag="VKZ48_01685"
     CDS             complement(6219..7541)
                     /locus_tag="VKZ48_01685"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HLU76597.1"
                     /translation="MKAAGATASPMTAREASARMRAGRLSARAYAEVCLARIAAREDS
                     LQAWVGFDPARALRLAEDCDAARAADEDRPLYGLPIGVKDLLATADLPTQMGSPIYAG
                     HRTQSDAEVVRRLQRAGAYVMGKTVTAEFAYLHPGPTRNPWNPAHTPGGSSSGSAAAV
                     AAGFVPLAIGTQTNGSIIRPAAYCGVVGFKPTAGTVPGNGCFVFSATLDQIGVFSADV
                     ADAASFASVLSDDASLADTAAPQRAPRFVALSRLPWCETEPEAAAHFDATLDHLRWAG
                     ATIERIDLPAPLAEGRSVHRTIMCYEAAREHAAMHAQRRGELSAILLTALDEGAAISP
                     TQYREALARRAAMIEAARDLFEDCDALLSPSAHGAAPHGIHSTGDPGFATLWSLLGIP
                     VISIPSGRSAAGLPFGLQIGATSGADAALLGVAQWCETQLAGRAKERT"
     gene            complement(7538..8524)
                     /locus_tag="VKZ48_01690"
     CDS             complement(7538..8524)
                     /locus_tag="VKZ48_01690"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HLU76598.1"
                     /translation="MKPRVLVTRANFPEVLDLLRAHFEVEDNQADEALDTEALRKRLS
                     DKDGALFLPIDRVDAHILQGSPRLKAACNVAVGYNNIDVAACTTAGILVTNTPDVLTE
                     TTADLGFALMMAAARRMGEAERHVRAGQWDGWAIEQFLGQDIHGATLGIVGMGRIGQA
                     IARRARGFDMQVLYHNRNRVPEETEDALQARYVSLDDMLREVDFLMLTAPYSAATHHL
                     IGAPQLAAMKPTAVLVNLGRGGVVDDAALVDALRAGTIFAAGLDVYEGEPALHRGFYE
                     LENVVLTPHIASATRATRIRMSMTAANNLVAALTGKRPPNLVNPEVWDRRRQ"
     gene            complement(8606..10018)
                     /locus_tag="VKZ48_01695"
     CDS             complement(8606..10018)
                     /locus_tag="VKZ48_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HLU76599.1"
                     /translation="MSGWMAPLMFGGLVLFLLAGYPVAFALAAVGLAFGLYAVQAGYF
                     DFALLQAMPERVFGILSNELLLAIPFFTFMGAVLQRTGLAEDLLEGIGQLFGPVRGGL
                     AFAVIIVGAIFGAITGTVAASVIAMGVIALPIMMRYGYSTRVATGVVAASGTITQIIP
                     PSLVLIVLADQLGRSVGDMYAGAIGASLMQVLLFCAFIALLTFIRPQAVPALPPEART
                     LKGRALALKIVAGVVPSLGLMFLVLGTILLGLATPTEGGAMGAVGALLVAAAHRRLNW
                     PMVWQAGEATMRLTAMVIFILIGSTVFALVFRGLDGDLWVERLLTGLPGGVVGFLVAV
                     NALVFVPAFFLDFFEIAFIIIPLLAPVAAKLGIDLVWFGVLLGANMQTSFMHPPFGFA
                     LFYLRGIAPPEIKTSDIYWGALPWVGLQLILVAILIAWPELVTGMLDHVEIITPGDAV
                     IDIDAPVYDWGGFDGYPAGP"
     gene            complement(10015..10554)
                     /locus_tag="VKZ48_01700"
     CDS             complement(10015..10554)
                     /locus_tag="VKZ48_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HLU76600.1"
                     /translation="MQFLLALSRRIDGLSARVGVVATACILLACAISAGNAVFRYAFD
                     LSSNAWLEAQWYLFAGVVMLGAAHTLRRNEHVRVDLLYGFASPRVRAWIDVLGTVFFL
                     FPAIGLMAWFAWPMFLNSFLSGEVSSNAGGLLRWPVKLLLPLGFSLLFLQGVSELIKR
                     VAQLRGVDIALDQYERPEQ"
     gene            complement(10863..12929)
                     /locus_tag="VKZ48_01705"
     CDS             complement(10863..12929)
                     /locus_tag="VKZ48_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HLU76601.1"
                     /translation="MRKELWFGFTLMGLLLLGLAIAMPSPADWTLGHYGLLMLALIVV
                     AIMLGFPTAFTLMGMGVIFAWFAYRAGTPDNALRLTLDLMVQRTYAVMTNEVLIAVPL
                     FVFMGYLVERANLIEKLFKSLHLALSRLPGSLAVATVVTCAIFATATGIVGAVVTLMG
                     LLAFPAMLRAGYNVSLSAGAITAGGCLGILIPPSVLLIVYGATAGVSVVQLYAGAFFP
                     GLLLAGLYVFYIIFLAKWRPRLAPPLSDEERIVPLSPEIAKVRDTTGQRAVPSLVQAL
                     TTKVPGAPTRTVANHLIVALLPAAVFLGAMGLMYDSLTTPAETYSTEGLVEMGGGWGF
                     EDDIESSLDLAPPPGVEATELGGETPTDEVTPSDEAETDEGDGALEAPKWFWITLACG
                     LFGLFAFYVIFDFARLEIFKMLLTSFFPLAVLILAVLGSIVFGFATPSEAAAVGGFGG
                     FALAAFYLLVNEEQARRKKIMRLWLPLWAIFFVPILWFAFADDLPPEWLGITAACALG
                     LWLILATVQVRLTGILKESVYLTAKTSAMVCWLFVGSSIFSAAFALLGGQELVERWVL
                     GMDLTPIQFMLMAQVIIFLLGWPLEWTEIIVIFMPIFIPLLPHFGIDPLFFGLLVALN
                     LQTAFLSPPVAMSAFYLKGVAPPHVTLNQIFYGMLPYMAIQIFAMFLLYMFPGIGLWL
                     PEVLYS"
     gene            complement(12926..13558)
                     /locus_tag="VKZ48_01710"
     CDS             complement(12926..13558)
                     /locus_tag="VKZ48_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HLU76602.1"
                     /translation="MQKLLFAVDRLSTFAGQLFAWTIVSLTLMICYEVFARYVLGTPH
                     SWVYDASNMMYGIGFMMAGAYTLAKSGHVRGDILYGFFTPRVQASIDLVLYIVFFIPG
                     VIALVWAGIDFFNESYAQNEHSSLAADGPPIYPFKAFIPIAGAVLLLQGFAEIVRCIV
                     CLKRGTWPAREEDVEEVDVEHLKAMVHVDDRDLAAADRALQAQEQAGAKQ"
BASE COUNT         2686 a         4520 c         4217 g         2143 t
ORIGIN      
        1 tgatcgcttc gggcgccatc ttctcgttcg accactggac gatctcatcg atcgtcggcg
       61 ccggcggacg cggcgtgccc atcgacgcac aaccaccgag cagcagaact gccgccagca
      121 gtactgtcag cattcgcatc attgcagacc tcaacggcaa acatcgtacc cgtcggcacg
      181 ctgacaagta tgcaacttca cttgacgatt gcaatgccgc aaaggagcga atattgccca
      241 tcgcagcatc gggggcgccg tggaggacgt caacccgctc ttataattcg ctccgcttct
      301 gaggggattc aaccatgcgt ttcagccgtc tttgcttcgg cctgttgttc gtgctttcgc
      361 ttttcgcggc gcgtgcgcaa gccgccacgg aaattcagtt ctggcattcg atggagggcg
      421 cgctgggcga ggcgctgcaa acgctcaccg acgatttcaa cgagcaacaa tccgaataca
      481 aagtcacgct tgcctacaag ggcagctatg acgaagcggt aggcgcaatc cgcgccgctt
      541 tcctcgaagg caagtcaccg catctggtgc aggtctacga tctgagcacg gcgaacctgc
      601 tcgcctccaa gcagatcatc cgcccggtgt acgaggtgct gcgcgaagcg ggcgaacgca
      661 tcgatgatca cgcctattcg ccggccgcgt cgagctacta ctccgacagc cggggcaagc
      721 tgatcggtct gccgttcaac gtcgcaacgc cggtgctcta ctacaaccgc gacatcctca
      781 agcgcgccgg actcgatcca aatccttcat acacgacctg gtacgacctg caggaggcgc
      841 tgctgaaggt ccggcatacg ggtgcaacag gctgcgcgct gaccacgacc atgcccgcct
      901 gggtgttgct ggagaacacg ctgacctggc acagccagga gttcgccacg cgcaacaacg
      961 gtttcgacgg gctggacgcc aagctgtcgt tcaacacgcg gcttgccatc cgccacatct
     1021 ccctgctgtc ctcgtgggtg aaggcagagc tgttcaccta cgccggccgc cgcgacgaag
     1081 gtgcggccat gttcaagcgt ggcgaatgcg cctttctcac cgcgtcgtcc tccgcctacg
     1141 cgaacatcga tcacgaagcc aaattcgatt tcggcgtact gcccatgccc tattacaagg
     1201 acatcaacaa tgcgccttac aacaccgccg tcggcggtgc cggcatctgg gtgatgggcg
     1261 gcaggaaacc ggcggaatac aaaggcgtgg cgaagtttct cgtctatctg gccaaacccg
     1321 cgacgcaggc gcagtggcat caggcaacgg gctatctgcc gctatcgcgc gccgcatacg
     1381 aactgacgaa aaagaccggt ttctacgacc agcgcccggg cacggagatt cccatccaga
     1441 cggtcacgca cgccaaccgt ccgaccgcct actcccgcgg cgtgcgactg ggcaactacg
     1501 cgctgatccg cgccatcatc gacgaagaac tggaagccgt atgggcacag aaaaaatcgc
     1561 ccaaggccgc gctggacacg gcggtcaagc gcggcaacga agtgctgcgc cgcttcgagc
     1621 gaggcagtaa gctctagcgc agaggcatcg aggccgccgg cggaccggcc ggcggcctcg
     1681 cagtcgctcg cgcgtccatc cgcgcgggtg acgatacgca ggggagagga tcttcgtaaa
     1741 gttcctgggc ggcctgtcgc cgcgttggtt cgcctttttg cttaacctgt ggccgccgtt
     1801 ccggtgcgca gggattcgcg tgcgccgcat ctccgccgac tggcgcgtca tcgacgtcga
     1861 gttgcggctc ggtctgctca atcgcaacta catgggctcg catttcggcg gatcgctgtt
     1921 ctccatgacc gatccgttct tctggctcat cgtcatgcgc gcattgggac cggattacgt
     1981 cgtctcgcac aaaggcggcc gcatcgattt tttcaagccc ggccgttccc acgtgcgggc
     2041 gcatttcgaa gtcgacgatg aggcgctgga cgacatccgc gcccgcacgg cgaacggcga
     2101 gcgcgacctt cccgaattca gcacggacat cgtggacgaa aacggcgagg tgatcgcacg
     2161 cgccacgcag atcctccaca tacgccgcaa gaccgccgtc taagttaatt cgaagcccat
     2221 cagcacggcg ccaccgagaa tggcgaatag caccgcagcg ctgtagcgga tcgccttgaa
     2281 cggaatcttc gctgctgcct tgtccgcaag gaacacgacc ggcgcgttgg cgaacagcat
     2341 gccgagcgtg gtcccgccga ccaccgcaac gagcgcctcg tacttcgctg ccagcgcgat
     2401 ggtcgccagt tgagtcttgt cgccgatttc ggccagaaag aaagccacca ggctgcacat
     2461 gaacacgccg taacagccga gcgtgccggg cgcctcgtcc agcttgtcgg gcttcaccgc
     2521 ccaggcggcg atgccgagga aggaggcccc cagtccccag cgcagggtgt ccggctcgag
     2581 cgcctcacgt atccagttgc cggccagacc ggccacggca tgattggcca gtgtcgccac
     2641 caggatgccg agcacgatcg gcaccggttt gcgaaaacgc gtggcaagaa tgaaagcgag
     2701 cagttgagtt ttgtccccga tttcgccgat cgtgacggcg agggtcgaga cgaaaaaggc
     2761 ttccagcatt gtgcatccgg cgtcgtacta cggtctcgcc ggcacgatac cgaacgcacc
     2821 atggctgcac ggccagggct gttgatgcgc tctccccgcg agtgcgaggc ggctactccc
     2881 cgtgaaacgg cgcgattcta acgccgcgta agtcgccaaa acggcgaacc ggagagggat
     2941 cgcaaaaata ccggcccgcc ttctcgaatt gttgtcagaa cggcaactcc acatccggct
     3001 tgaccgccag cagcagactg cggaagtcgt cctggatgcg cttcaggccg gcctggtcct
     3061 tcgcctcgaa gcgcagcacg atcaccggtg tagtgttcga cgcgcgcgcg aggccgaagc
     3121 cgtcctcgta ttccacgcgc agaccgtcga tggtgatgac ctgcgtcgca tcggggaaat
     3181 tcgcgccggc ctgcagttgc gtaatcatcc ggtgcggctc gccttcgcgc agcttgatgt
     3241 ttagttccgg cgtcgtgacg gaatcgggca gcgccttgag cggcgcgctg gcgtcggatt
     3301 cgcgcgaaag gatttcgagc agacgcgcgc ccgcgtaaag gccgtcgtcg aaaccgtacc
     3361 accgctcttt gaagaaggtg tgcccgctca tctcgccggc cagcggcgca ccggtttcgc
     3421 gcagcttcgc cttgatgaag gaatgccccg tgcgccatag caccggctcg ccgccgtgct
     3481 gtttgatcca gggtgcgaga ttggtggtgc acttgacgtc gtaaatgatc ttgccgcccg
     3541 gcacgcgggt gagcacatcc ttggcgaaca gcatcagttg ccggtcggcg tagatcactt
     3601 cgccatcctt ggtcaccacg cccagacggt cgccgtcgcc gtcgaaggca agaccgagct
     3661 cggcatcgga ctcccgcagc agcttgatga cgtccttgag gttgtcgggc tgcgagggat
     3721 cggggtggtg attggggaag gtgccgtcca cttcgcaata gagttcatgc accttgcagc
     3781 ccatgcgccg atacagttcc ggcgcaagac tgcccgccac gccgttcccg caatcgacaa
     3841 tgaccgacat cggtcgcgcc actttgacgt cgccgacgat ttcgtccaga tagtccgcgc
     3901 tcacgtcctg ctcgatatga cgcccttcgc cctgggcgaa atcgccgcgc tcgatgcgct
     3961 cgcgcaaggc ctggatctcc tcgcccgaca gcgtcacgcc ggcgacgacc attttcaggc
     4021 cgttgtactc cggcgggttg tgactccccg tcactgcgac ggcgctgccg gtgtcgagct
     4081 tgtgcgccgc gtagtacacg accggcgtgg gcaccatgcc cacgtcgtat acgtccacac
     4141 ccgccgcacg cagccccttg ctcaacgcct gcaccagttc cgggccggaa agccggccgt
     4201 cgcgcccgac ggcgaacgcg ctgccgccgc gctcgcggca aagcgtgccg agcgcacggc
     4261 cgacgagttc gacgacttcg ggggtcagcg tcttgccgac gatgccgcgt atgtcgtaag
     4321 ccttgaagat ttcctgcggt acctggttca tgatcgatcc gcctgattga agaattcgaa
     4381 aacccgctgc ggcaaccagc gcaaatgccc cgggaaaggg ccgctgagaa agcccacatg
     4441 tccgccgctt acaggcagtt ccaaggtcac ggccgccgaa acctcatcgt gtgccggcag
     4501 atgcttcgcc ggcagaaagg ggtcgttgcg cgcgttcatc accagcgtgc gcacgcgaat
     4561 gtcgcgcagc agcggtttgg cgctggcgcg cgtccaataa tcggtcacat cggcaaagcc
     4621 gtgcaacggc gcggtcaccg cgtcgtcgaa ctcgcgcagc gtgcgcgcac gcgccgcgcg
     4681 ctcgccgtcg aaggcaccgg gaaaacgcgc aatgatgtcc atgctcttgg ccaccagcgt
     4741 agcaaggaac atgcgggtgt aaatcctgtt gaagccgcgc tcgagcgcat gacccgccgc
     4801 catgagatcg agcggcgccg acacggcggc cgcggcccgc accagttcgt tcgcggcggc
     4861 accctgctcg cccagccact tcagcagcac gttgccgccc agcgagacgc ccatcgcata
     4921 catcggaccg tcgcccgcaa tcgcctgtgc acggcgcagc atccagtcga gttcgaccgt
     4981 atcgcccgag tgataggcgc gcggcagacg attcgcttcg ccgctgcagc cgcgaaaatg
     5041 gggcagcagc acacgccagc cgcgcgcgcg cgcaacctgc atcagtgcgc gggcgtaatg
     5101 gctgtgcgag ccgccttcca gcccatgaaa cagcacgagc agaggcgcgc tcgccgacgc
     5161 ctcgagcaga tcgacatcga caaagtcgcc gtcgggggtg tcccagcgtt cccgcagcgt
     5221 cggggtccgc ggcgggcggc gaaagagaaa aggatagata gtctgcagat gtccgccggg
     5281 cagccaccac ggcgcgcgat aggcggacga ggcatgaggc gcgcgctcgt cggcaagtac
     5341 gacgccgttg gccgtctgtg cgccgaggtc gccctgcttc attgcgaatg cttccccacc
     5401 ggcttcaatg cagcctcttc agcgcctcga ccggcagctc ggaaggcacg ggcgccgccg
     5461 acgcatggtg catcagcatg cgccagccgt cgctggtgcg catatagacg ttcgttgcca
     5521 tgaacggcgg ccgcgccttg ggctcgcccg ccaccagcac atgctcgtgc acgctgtgca
     5581 ccgcgacgag catgccctgc atataaatct gattcgacag atgcacgcgc agccgctcgc
     5641 gtccgtcgaa catttccgca tagctcttgc gcacctgttc gaaccccgtc aggcgcggtc
     5701 cgcccggatg gatgcagacg acctcctcgt catcggccca cacgttcatc atcgcggcga
     5761 gatcgccgcg ctcgaacgct tcgtagaacg cagcctccgc atcctgtgcg gtggcgaagc
     5821 cgctgcgttt ggtcgcgcgc tgcatgatca ctccctcaac gactcggccg ccgcccaatc
     5881 cgcgatggca tcgagcagcg tttcgctttc gccgatgggc ggcagcacgc ggatgcgcag
     5941 ttgcggatga cgcgccagcg ccgcatccac cagttgcggc agatcgttgc gcagatgacc
     6001 gcccgctgcg aggaacaggg gcacgacgct gatgcgctcg accgactgcg caaccagttc
     6061 atcgaccgcg gcctggaagt cgggctgcat caattccaga taagcgagcg tcacgcgcgc
     6121 atttttgccc cgcgccgcca cgcgctcgcg aatgcgcgta aacggtgctg cccactcggc
     6181 cgaacggctg ccgtgcgcaa acagcacgat gccgaggctc atgtgcgctc cttcgcccga
     6241 ccggccaact gcgtctcgca ccactgtgcg acaccgagca gtgccgcgtc cgcaccgctc
     6301 gtcgcgccga tctgcaggcc gaaaggcagc cccgccgccg accggccgct ggggatggag
     6361 atgaccggaa taccgagcaa cgaccacagc gtggcaaaac ccggatcgcc cgtcgaatgg
     6421 atgccatgcg gtgcggcgcc atgcgcagac ggactcaaca gagcgtcgca atcctcgaac
     6481 agatcgcgcg cggcttcgat cattgcggcg cgacgtgcga gcgcttcgcg atactgcgtc
     6541 gggctgatcg ccgcaccttc gtccagcgcg gtgagcagga tggcgctcag ttcaccgcgg
     6601 cgctgtgcgt gcatcgcggc atgttcgcgc gccgcctcgt agcacatgat ggtgcggtgc
     6661 accgaccgcc cttcggcgag cggcgccggc aagtcgatcc gctcgatcgt cgcaccggcc
     6721 catcgcagat ggtcgagcgt tgcatcgaaa tgcgcggccg cctccggctc cgtctcgcac
     6781 cagggcaaac gcgacagcgc gacaaagcgt ggcgcacgct gcggcgccgc tgtatccgcc
     6841 agcgacgcat cgtcgctcag cactgacgcg aacgatgcgg catccgcaac gtccgccgaa
     6901 aacacgccga tctggtcgag cgtggcgctg aacacgaaac agccgttgcc gggcaccgtg
     6961 cccgccgtcg gcttgaaacc caccacgcca caatatgcgg cagggcgaat gatcgaaccg
     7021 ttggtctgcg tgccgatcgc cagcggcacg aaccccgccg ccaccgctgc cgccgatccc
     7081 gacgaggaac cgccgggcgt atgcgcaggg ttccaggggt tgcgcgtcgg acccggatgc
     7141 aggtaggcga actccgccgt cacggtcttc cccataacgt aggctcccgc gcgctgcaac
     7201 cggcgcacga cttccgcatc gctctgggtg cgatgacccg cataaatcgg tgagcccatt
     7261 tgcgtgggca ggtcggcggt ggcgagcaga tccttgacac cgatgggcaa gccgtaaagc
     7321 ggtcgatcct catcggccgc acgtgcggcg tcgcagtcct cggccaggcg caaagcgcgc
     7381 gccggatcga atccgaccca cgcctgcagg ctgtcttccc gcgcagcgat gcgcgcgagg
     7441 cagacttcgg cgtaagcgcg tgcgctcaat cggccggcgc gcatccgggc gctcgcctcg
     7501 cgcgccgtca tcggcgacgc cgtcgcaccc gcagctttca ttgacgacgc cgatcccaca
     7561 cttccggatt gacgagattg ggcggccgct tgccggtcag cgccgcgacc agattgttcg
     7621 ccgccgtcat gctcatgcgg atacgcgtgg cacgcgtggc actggcgata tgcggggtca
     7681 ggacgacgtt ttccaattcg tagaagccgc gatgcaatgc cggctcgcct tcatagacgt
     7741 cgaggcccgc agcgaaaatg gtgcctgcgc gcagcgcgtc caccagcgcg gcgtcgtcca
     7801 ccacgccgcc gcgaccgagg ttcaccagca ccgccgtcgg cttcatggcg gccagttgcg
     7861 gcgcaccgat caaatgatgc gtggccgcgc tgtaaggtgc agtcagcatg aggaaatcca
     7921 cttcgcgcag catgtcgtcg agcgacacat agcgcgcctg caatgcgtcc tcggtctcct
     7981 ccggcacgcg attgcggttg tgatagagca cctgcatgtc gaagccgcgc gcgcgacgcg
     8041 caatggcctg gccgatgcgc cccatgccga cgatgccgag cgtcgcgccg tggatgtcct
     8101 ggccgagaaa ctgttcgatc gcccagccgt cccactgccc cgcgcgcaca tgccgttccg
     8161 cttcgcccat gcggcgcgcg gccgccatca tcagcgcgaa gcccagatcg gccgtggttt
     8221 cggtcagcac gtcgggcgtg ttggtgacca ggattcctgc ggtcgtgcag gcggcgacgt
     8281 cgatattgtt gtagcccacc gccacgttgc aggcggcctt gagacgcggg ctgccttgca
     8341 gaatgtgggc gtcgacgcgg tcgatgggca ggaacagcgc cccgtccttg tcgctcaacc
     8401 gcttgcgcag ggcttcggta tccaaggcct catcggcctg gttgtcttcc acttcgaagt
     8461 gcgcgcgcag caaatccaac acttccggaa agttggcgcg cgtgacgagt acacgaggtt
     8521 tcattgctgg accgtaggct gccggaggga caataaccat atcacgctcg cccggccgtg
     8581 ccatcgcgcg tcgcgcgact gcgtctcagg gtccggcggg atagccgtcg aaaccgcccc
     8641 agtcgtagac cggcgcgtcg atgtcgatca ccgcatcgcc gggcgtgatg atttccacgt
     8701 ggtccagcat gccggtcacc agttccggcc aggcgatgag gatcgccacc aggatcagtt
     8761 gcagccccac ccagggcagc gcgccccagt agatgtcgct ggtcttgatt tccggcggcg
     8821 caatgccgcg cagatagaac agcgcgaagc cgaaaggcgg atgcatgaag gatgtctgca
     8881 tgttcgcgcc cagtagcacg ccgaaccaca ccagatcgat gccgagcttc gccgccaccg
     8941 gcgcaagcag cggaatgatg atgaacgcga tctcgaagaa atcgagaaag aaggccggca
     9001 cgaacaccag cgcattgacg gccaccagaa aacccaccac gccgccgggc agcccggtca
     9061 gcagtctttc gacccacaga tcgccgtcga gcccgcgaaa caccagggcg aacacggtcg
     9121 aaccgatgag gatgaaaatc accatcgcgg tgagccgcat ggtggcttcg cctgcctgcc
     9181 acaccatcgg ccagttgagg cgtcgatgcg ccgccgcaac cagcaacgcg cccaccgcgc
     9241 ccatggcacc gccttcggtc ggtgtggcca gccccaacag aatcgtcccg agcacgagga
     9301 acatgagacc gagcgaaggc acgacgcccg ccacgatctt gagcgccagc gcgcggccct
     9361 tgagcgtgcg cgcctccggc ggcaatgcgg gcaccgcctg cggccggatg aaggtgagca
     9421 gcgcgatgaa agcgcagaac agcagcacct gcatcaggct cgcgccgatc gcgccggcat
     9481 acatgtcgcc gaccgaacgg ccgagctgat cggccagcac gatgagcacc agcgagggcg
     9541 gaatgatctg ggtgatggtg cccgatgcag ccacgacgcc ggtcgccacg cgcgtcgaat
     9601 agccgtagcg catcatgatc ggcagcgcga tgacgcccat ggcgatgacc gacgccgcca
     9661 ccgtgccggt gatcgcaccg aaaatcgcgc cgacgatgat gacggcgaag gccagcccgc
     9721 cgcgtaccgg accgaagagt tggccgatgc cctcgagcag atcctccgca agccccgtgc
     9781 gttgcagcac cgcgcccatg aaggtgaaga agggaatggc aagcagcagt tcgttggaca
     9841 gaatcccgaa cacccgctcg ggcatggcct gcagcagcgc gaaatcgaaa taaccggctt
     9901 gcacggcgta cagcccgaac gcgagtccga cggccgcaag cgcgaacgcg accgggtagc
     9961 cggcgagcag aaacagcacc aggccgccga acatcagcgg cgccatccag ccgctcattg
    10021 ctccggtctt tcgtactgat cgagcgcgat gtccacaccg cgcaactgcg ccacgcgctt
    10081 gatgagttcg cttacgccct gcaggaacag cagggagaaa ccgagcggca gcagcagctt
    10141 caccggccag cgcaacagtc cgccggcgtt gctcgagact tcgccgctca ggaaggaatt
    10201 gaggaacatc ggccaggcga accacgccat gaggccgatc gcgggaaaga gaaagaatac
    10261 ggtgcccagc acgtcgatcc acgcgcgcac gcgcggcgag gcaaaaccgt agagcagatc
    10321 cacgcgcaca tgctcgttgc gtcgcagcgt gtgcgccgca ccgagcatca ccacaccggc
    10381 gaacagatac cactgcgcct cgagccaggc attggaactc aggtcgaagg catagcggaa
    10441 gacggcattc cccgcactga tcgcgcaggc gagcaggatg caagcagtgg cgacaacgcc
    10501 gacgcgtgcg ctcagtccgt cgatgcggcg gctgagcgcc aacaagaact gcactggatt
    10561 cctccccggc ccgctgatgg actattcgat atgggccgag ggcgatttta cgagaggcac
    10621 gagcaacaaa gcacccgaaa ctccaaaaag ctgaaacacg gggatcgcgg agagcagaga
    10681 aaccttaaaa cctggaaagc ctgtggcttc ccatcgtcat gcctgcacag gcgggcatcc
    10741 gggtcttact ctgtgtcctc tgtggtgcaa gatttcaaga ggcgcccgca ccgcacagac
    10801 gttagtgcgg tgcgactcca ctcgccgctc ccgactctcc actctcgact ctcgaccgcc
    10861 tcctagctat acagcacttc cggcagccac aggccgatgc ccgggaacat atagagcagg
    10921 aacatggcga aaatctggat cgccatataa ggcagcatgc cgtagaagat ctggttcaag
    10981 gtcacatgcg gcggcgccac gcccttgaga tagaacgccg acatcgccac cggcggcgac
    11041 aggaaggcgg tctgcaggtt gagcgccacc agcaagccga agaacagcgg gtcgatgccg
    11101 aagtgcggca gcagcggaat gaagatcggc atgaagatga cgatgatctc cgtccactcc
    11161 agcggccagc ccagcaggaa gatgatcacc tgggccatca gcatgaactg gatgggcgtg
    11221 agatccatgc cgagcaccca gcgttccacc agttcctgac cgcccagcaa tgcgaacgcc
    11281 gcggagaaga tcgacgaacc gacgaacagc cagcacacca tggcgctggt cttcgccgtg
    11341 aggtagacgg attccttcag aatgccggtg agccgtacct gcacagtggc caatatcagc
    11401 cagaggccga gcgcacaggc cgccgtaatg cccagccact cgggcggcag atcgtcagcg
    11461 aaggcgaacc agagaatggg cacgaagaaa atcgcccaca acggcaacca caagcgcatg
    11521 atttttttgc gtcgtgcctg ctcttcattg acgagcaggt agaaagcggc cagggcgaag
    11581 ccgccgaacc cccccaccgc cgccgcttcc gaaggcgttg cgaagccgaa cacgatggag
    11641 ccgagcacgg cgaggatcaa caccgcgaga ggaaagaaag atgtcagcaa catcttgaag
    11701 atttccagcc gcgcgaagtc gaaaatcaca tagaaggcga acaacccgaa caagccgcag
    11761 gccagcgtga tccagaacca cttgggcgcc tcgagtgcgc cgtcgccttc atccgtttcg
    11821 gcttcatcgc ttggcgtcac ttcatcggtt ggcgtctcgc ctcccagctc ggtcgcttcg
    11881 acgccgggcg gcggcgcgag gtcgagcgag gactcgatgt cgtcctcgaa accccagccg
    11941 ccgcccattt ccaccaagcc ctcggtggaa taggtttcgg ccggcgtggt gagcgagtcg
    12001 tacatgaggc ccatggcacc caggaaaacc gctgcgggca acagcgccac gatgaggtga
    12061 ttggcaaccg tacgggtggg cgcgcccggc actttggttg tcagcgcctg taccagcgag
    12121 ggcacagcgc gttgcccggt ggtgtcgcgg actttggcga tctccggcga cagcggaacg
    12181 atgcgctcct cgtcggacag cggcggcgca agccgcggcc gccacttcgc cagaaagatg
    12241 atgtagaaca cgtacagtcc ggccagcagg agtccgggaa aaaatgcgcc ggcgtagagt
    12301 tgcaccaccg acacaccggc cgtcgcgccg tacacgatca gcagcaccga aggcgggatg
    12361 agaatgccca gacagccgcc ggcggtgatc gcgcctgccg acagcgaaac gttgtagccc
    12421 gcccgcaaca tcgccggaaa ggcgagcagg cccatcagcg tcacgacggc accgacgatg
    12481 ccggtcgccg tggcaaaaat tgcgcaggtg acgaccgtcg caacggcaag cgaacccggc
    12541 agccgcgaca gcgccagatg caggctcttg aacagctttt cgatcaggtt ggcccgctcg
    12601 accaagtagc ccatgaacac gaacagcggc accgcaatga gtacctcgtt cgtcatgacg
    12661 gcgtaggtcc gctgcaccat cagatcgagg gtcagtcgca aggcgttgtc gggcgtgcct
    12721 gcgcggtagg cgaaccaggc gaagatcaca cccatgccca tcagcgtgaa ggcggtcggg
    12781 aagcccagca tgatcgccac gacgatcagc gcgagcatca gcagtccgta gtggcccagc
    12841 gtccagtcgg cgggggaggg catggcgatc gccagaccaa gcaacagcag gcccatgagg
    12901 gtgaagccga accagagctc ttttctcatt gttttgctcc ggcttgttcc tgtgcctgca
    12961 gtgcacggtc ggcagcggca aggtcgcgat catccacatg caccatggcc ttgaggtgct
    13021 cgacgtccac ttcctccaca tcctcttcac gcgcgggcca cgtgccgcgc ttgaggcaca
    13081 cgatgcaacg gacgatttcg gcgaagccct gcaacagcag cacggcgccc gcgatgggaa
    13141 tgaaggcctt gaacggatag atcggcggtc cgtcggcggc gagcgaggaa tgttcgttct
    13201 gggcgtagga ctcgttgaag aaatcgatgc ccgcccacac cagcgcgatc acgccgggaa
    13261 tgaagaacac gatgtacagg acgaggtcga tggacgcctg cacgcgcggg gtgaagaagc
    13321 cgtacaggat atcgccgcgc acatggccgc ttttggccag cgtataggcg ccggccatca
    13381 tgaagccgat gccgtacatc atgttgctcg cgtcgtacac ccaggagtgc ggcgtgccga
    13441 gcacgtaacg ggcgaacacc tcgtagcaga tcatcagcgt gagcgaaacg atcgtccacg
    13501 cgaacaactg gccggcgaag gtgctcagcc ggtcgaccgc aaacagaagc ttttgcattc
    13561 ggaaac
//
LOCUS       DATGOU010000029        11945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3173117, whole genome shotgun sequence.
ACCESSION   DATGOU010000029 DATGOU010000000
VERSION     DATGOU010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11945
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3173117"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2063)
                     /gene="flhA"
                     /locus_tag="VKZ48_01715"
     CDS             complement(<1..2063)
                     /gene="flhA"
                     /locus_tag="VKZ48_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017878899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HLU76603.1"
                     /translation="MNAAVMNGMFGNGQLRGMAGPLLIVMILSMMILPLPPWLLDLLF
                     TFNIALGLMVLLTSMYTTKPLDFAAFPTILLLTTLLRLSLNVASTRVVLLHGHTGPDA
                     AGKVIEAFGHFLVGGNFAVGLVVFVILVVINFVVITKGAGRIAEVSARFTLDAMPGKQ
                     MAIDADLNAGLIGEAEARQRRAEIAQEAEFYGSMDGASKFVRGDAIAGILILLITLVG
                     GLVVGVMQHDLSMGEAGKQYVLLAVGDGLVAQIPALVISTAAGLVVSRVGTGSDLSEQ
                     VLAQLFARCQVLNLAAGVLFLLGIIPGMPHFAFLFFGSALAALAYWLKLRDQQPAPAA
                     EPEPVAQPEAVQEATWDDIVPVDTLGLEVGYRLIPLVDKAQDGELLRRIKAIRKKFAQ
                     EVGFLPPAVHIRDNLELKPAGYRITLKGVVIGEGEAFPGQFLAINPGRATGTLPGAAT
                     KDPAFGLPATWIDASQRDRATTSGFTVVDAGTVIATHLSHLVTQNAAALLGRAEVQQL
                     IDHFSRATPKLIDDLVPKLIPFSTLQKVLQNLLAEGVHVRDLRTIVETLTDHAAHTQD
                     ATELTGAVRIALGASIVQQIYGSAPELSVIVIEPELEKVLNQALAAEGFGLEPGLAET
                     LLREAGAAARHQEALGLPTALLVPDRMRAQLARLLRRALPQLRVLAHAEIPDSRNVRV
                     SSVLGG"
     gene            complement(2060..3220)
                     /gene="flhB"
                     /locus_tag="VKZ48_01720"
     CDS             complement(2060..3220)
                     /gene="flhB"
                     /locus_tag="VKZ48_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HLU76604.1"
                     /translation="MAAESDQEKTLPATPRRLEQAREEGQVARSRELGACAVVLSGAA
                     LAWTAGSAAVDYSIRLMRAGLTVDRESAFSTAAVGERFLSLGIDAFLGLVPLLVTVVV
                     IAILSALLIGGWLYSPRAFQPDLGRMSPLKWVKRTFSAEGLAELGKAVAKALLVGTIG
                     CLFLWLHREEMLGLIMLEPGQGLAEFGGMVQGSLLALAAALALIALIDVPLVLWKHHK
                     GLRMSLEEVKREMKESEGDPQLKARIRSQQREVARRRMMAEVPKADVVVTNPTHFSVA
                     LAYREGANGAPRVLAKGRGEIALKIRELAAAHEVPLLEAPPLARALYASCELGDEIPP
                     ALYHTVARVLAYVYQLRRYRTQGGAYPHEPQDLEVPEGMDPGAPEDEEGAGR"
     gene            complement(3535..5196)
                     /locus_tag="VKZ48_01725"
     CDS             complement(3535..5196)
                     /locus_tag="VKZ48_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HLU76605.1"
                     /translation="MLENMRIGTRFKLLGALLMSMLIGGAGVGIFGVWLQHDKLNDVL
                     SEQDRVLVMLRNAEAARAHFRMQTLELKDAILRGADQDVFQQHTDSMRTHRQAAAKEL
                     LSLRDLASDEARDRVEAMIKQHADVSDRYDEALKLYEYFNPLGSAILIDGMLRGLDTP
                     VDKGLTDLRNAMEERFVAQATADSESAADNYRMTIIGFIVSTLVAMGAAWLVFSWITR
                     GLLNQLGGDPADAVAIARRIAGGDLTTPVAVHEGDDRSLLAAISDMQDHLRALIEHLR
                     DSAGALSGQATALSSSSVQIAASSEQQNEAASSMASSIEQMTASIGQVAEHSNEALSI
                     SRASGELSNRGDEVVQSAAAEMAQIAASAESMTQIIETLGAQSTQISRIVHVIQEIAN
                     QTNLLALNAAIEAARAGEQGRGFSVVAEEVRKLAERTTESTREIGDMIKAIQDGTGQA
                     VSHMETWSVRVADGMAKARGAGECMRDVKARASEVLSVVNEISHALSEQSSASSQIAQ
                     TVERIARMSEENAAAVGSVASSAQNLEEMARMLESVVARFRLSAA"
     gene            complement(5335..7155)
                     /locus_tag="VKZ48_01730"
     CDS             complement(5335..7155)
                     /locus_tag="VKZ48_01730"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HLU76606.1"
                     /translation="MNAFTDMQDLLLEFLSEAGELLDDVDLQLVELEGRADDLDLLNA
                     VFRGFHTIKGGAGFLEATALVTICHRAEALLDRLRSGKLRLTPDVLDYILAATGEVRR
                     MFGEMANLVQPDPAPEQLIAQLEALAQGESLPEAPAPAAAEVSATVASNADAVPAVAG
                     EPDWAALYAAVSGKAVAAQPAAAAKETKPVEAPAPKAPAPKGNGAQKNGGAAKGGGAQ
                     KENTLRIDTQRFDQILNLSGEIGLTKNRLACIRRDLLRVGGEMETLKALDDAVNELDM
                     LVSDLQNSVMKARMQPVGRVFQKYVRLTRDLARQLGKEIELVLVGEDTEVDKTILEEL
                     NDPLVHLVRNAVDHGVEMPAERVAQGKPAKSIVRLAARQTGDQIIIEIADDGKGMRPE
                     VIRAKAVEKGVISPEEAVQLDDRQSLQLIFAAGFSTKEQASNLSGRGVGMDVVKTNIH
                     RLKGRIDLESVPGKGTTVSIVLPLTLAILPVLNLRLGMQPFAVPLAAVREIIELSDEV
                     IEYVSGKPHILMRGEVLPFVELSHLLNRPRDGLAPLGVVATVGDRTMVLGVDGLMGQD
                     EVMIKPLAGVKPRGVAGATISGEGSLVLVLELHELLDGVR"
     gene            complement(7444..8367)
                     /gene="cheZ"
                     /locus_tag="VKZ48_01735"
     CDS             complement(7444..8367)
                     /gene="cheZ"
                     /locus_tag="VKZ48_01735"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008368.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HLU76607.1"
                     /translation="MSGTETEDLEALFDEIAAQRAAEAQQPAPAAEEEDLEALFDQVA
                     AQSAAEQAAPAEEEDLEALFDRVAAERSEAEPFTKEDAPAPEVNEEPAADIVVGQAQS
                     AEPPPPQEVFQRIGALTRNLHDALHELGYDKAVEDAVSSLPDARDRLSYIADLTGKAA
                     ERALTAVEQGQQYQNEVRSEASRLAAQWERVFNGELELEEFKHTAVETRGFLKSLDDH
                     TARTNEQFTEIMMAQDFHDLTGQVIQRIVKVAQNLESQLVKLLIETTPPEKRPASESE
                     WLTGPAMNADERDDVVANQQQVDELLESLGF"
     gene            complement(8390..8776)
                     /gene="cheY"
                     /locus_tag="VKZ48_01740"
     CDS             complement(8390..8776)
                     /gene="cheY"
                     /locus_tag="VKZ48_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HLU76608.1"
                     /translation="MSTELKFLIVDDFSTMRRIVRNLLKELGYVNADEAEDGVVALAK
                     LKSGGFDFVVSDLNMPNMNGFELLRQIRADENLKVLPVLLVTAEAKKEDIVTAAQLGA
                     SGYIVKPFTKATLEEKLNKILQKQAA"
     gene            complement(8773..9726)
                     /locus_tag="VKZ48_01745"
     CDS             complement(8773..9726)
                     /locus_tag="VKZ48_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HLU76609.1"
                     /translation="MMTTDDRLLSVVEQKARLAGSNRMELLLFSLGGRETFGINVFKV
                     REVCEMKPITPTPNMPHGVEGIISLRGAIIPVIGLARCLGMEQTAEGGKLIITEFSSS
                     TQAFLVGEVDRIVRVDWEQVKSPQSTVTGGAGLVTAITELAGGRLVSILDVEQILVDV
                     MGEEEVPPLDAPVSDSSRMVFFADDSAIARKKVTQVLDQMGLTYQQAINGREALERLQ
                     LLAAQAQIEGVPLSERLGVVLVDAEMPEMDGYVLTRHIKQDPRFNGIPVVMHSSLSSV
                     ANKKLGQQVGVDAYVAKFHFQELAGTIAGMLRSGREQGSQA"
     gene            complement(9743..10693)
                     /gene="motB"
                     /locus_tag="VKZ48_01750"
     CDS             complement(9743..10693)
                     /gene="motB"
                     /locus_tag="VKZ48_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008114179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HLU76610.1"
                     /translation="MKRDPLQPIVIKRIKKVQPNHGSGMWKVAYADFVTAMMAFFLLM
                     WLLGSTTQGDLKGIQDYFNTPLKVALSGGSGSGDSSSVIQGGGNDLTRTHGQVKMGDI
                     GRTKIINLQAARAEIARDEAVKLKQLKERVEKIFDSNPRFSAYRNQIKLDITSEGLRI
                     QIVDDQNRPMFDNGRSVVHPWMKEILRELGGLLNDVENKISLAGHTDAKPYFTGDRGY
                     SNWELSADRANASRRELVAGGIAEDKIIRVVGLASSIPLNTADPFDEINRRISIVVLN
                     KKTEEMITSEGIAVQVPDAELANDMIHAVTPEIDPAGGGR"
     gene            complement(10701..11561)
                     /gene="motA"
                     /locus_tag="VKZ48_01755"
     CDS             complement(10701..11561)
                     /gene="motA"
                     /locus_tag="VKZ48_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HLU76611.1"
                     /translation="MLVIVGYIIVLGSVFGGFALAGGHLASMLAPLELLIIAGAAFGA
                     FVVGNNGKVLKATFKTLPSVLKSSRYTKALYMELMSLLYEILTKVRKEGLMSIERDVD
                     APQDSPMFTKYPAIAKDAHLIEFITDYLRLMVSGNLNPMEIENLMDNEIETHHNEAIV
                     PAHAIQRVGDGLPAFGIVAAVMGVVHTMGSVGLPPAELGKLIGHALVGTFLGILLSYG
                     FVGPLASKIEQGVAESNKMLECVKVTLLASLNGYAPALAVEFGRKVLYSTERPSFMEL
                     EQHVKTSLKR"
BASE COUNT         2021 a         3947 c         3724 g         2253 t
ORIGIN      
        1 ccgcccagca ccgaactgac gcgcacgttg cggctgtccg gaatctccgc atgcgcgagc
       61 acgcgcagtt gcggcagcgc gcgacgcagc agtcgcgcga gttgcgcacg catccggtcg
      121 ggcaccagca gcgccgtcgg caggcccagc gcctcctgat ggcgcgccgc tgcacccgct
      181 tcgcgcaaca gcgtttccgc caggcccggt tcgagtccga aaccttcggc ggccagcgcc
      241 tggttgagca ccttttccag ttccggttcg atgacgatga cggacagctc cggcgcggaa
      301 ccgtagatct gctgcacgat cgaagcgccc agcgcgatac gcacggcgcc ggtgagttcg
      361 gttgcatcct gcgtgtgcgc cgcgtgatcg gtgagcgtct ccacgatggt gcgcaggtcg
      421 cgcacgtgca cgccttcggc gagcaggttc tgcaacactt tctgcagggt ggagaacgga
      481 atgagcttcg gcaccagatc atcgatgagc ttgggcgtgg cgcgcgagaa gtggtcgatg
      541 agttgctgca cttcggcgcg gccgagcagc gcggcggcat tctgtgtgac cagatgcgac
      601 agatgcgtgg cgatgacggt gcccgcatcg acgacggtga agccggaggt ggtggcgcgg
      661 tcgcgctgcg aggcgtcgat ccacgtcgcc ggcagaccga aggccgggtc cttggtcgcg
      721 gcgccgggca gcgtgccggt cgcgcgcccc ggattgatgg cgaggaactg gccggggaag
      781 gcttcgcctt cgccgatgac cacgcccttg agtgtgatgc ggtagcccgc gggcttcagc
      841 tcgaggttgt cgcggatatg caccgcgggc ggcaggaagc ccacttcctg cgcgaacttc
      901 ttacggatcg ccttgatgcg gcgaagcagt tcgccgtcct gcgccttgtc caccagcgga
      961 atgagccggt agccgacttc cagcccgagc gtatcgaccg gcacgatgtc gtcccaggtc
     1021 gcttcctgta cagcctccgg ttgcgcgacc ggttcgggtt cggccgcggg cgcgggctgc
     1081 tgatcgcgca gcttcagcca gtacgccagc gcagccagcg cactgccgaa gaacagaaag
     1141 gcgaagtgcg gcatgccggg gatgatgccg agcaggaaca gcacgccggc tgccagatta
     1201 agcacctgac agcgcgcaaa caattgcgcc agcacctgct cgctcaggtc ggagccggtg
     1261 ccgacacggc tcaccaccag accggccgcg gtggaaatga ccagcgccgg aatctgcgcg
     1321 accaggccgt cgcccacggc gagcagcacg tattgcttgc ccgcctcgcc catcgacaga
     1381 tcgtgctgca tcacgccgac gaccagtccg ccgacgagcg tgatcaacag gatgagaatg
     1441 ccggcgatgg cgtcgccgcg cacgaacttg gacgcgccgt ccatggaacc gtagaactcg
     1501 gcctcctgcg cgatttccgc acgacgctgg cgcgcttccg cctcgccgat gaggccggca
     1561 ttgaggtcgg cgtcgatcgc catctgcttg ccgggcatcg cgtccagggt gaaacgcgcg
     1621 ctcacttcgg cgatacggcc ggcgcccttg gtgatgacga cgaagttgat caccaccagg
     1681 atgacgaaca ccaccaggcc gacggcgaag ttgccgccga cgaggaagtg gccgaaggcc
     1741 tcgatcacct tgcccgcggc atcgggcccg gtatggccgt gcaacagcac cacgcgcgtc
     1801 gaggcgacgt tgagcgaaag gcgcagcagc gtcgtcagca gcagaatggt cgggaaggca
     1861 gcgaagtcga gcggcttggt ggtgtacatc gacgtcagca gcaccatcag gccgagcgcg
     1921 atgttgaagg tgaacagcag atcgagcagc cacggcggca gcggcaggat catcatcgac
     1981 agaatcatca cgatgagcag cgggcctgcc atgcccctga gctgaccgtt gccgaacatt
     2041 ccgttcatca ctgcggcatt catcgtcctg cgccttcctc atcctcgggc gcgccggggt
     2101 ccatgccctc cggcacttcg aggtcctgcg gttcatgcgg atacgcgccg ccctgcgtgc
     2161 ggtagcggcg caactggtag acataagcga gcacacgcgc cacggtgtga tagagcgcgg
     2221 gcggaatttc gtcgccgagt tcgcagctcg cgtacagcgc gcgcgcaagc ggcggcgctt
     2281 cgagcagcgg gacttcgtgc gccgccgcga gctcccggat cttcagcgcg atttcgccgc
     2341 gccccttagc aagtacgcgc ggcgcgccgt tggcgccttc gcgataggcg agcgcgacgg
     2401 agaagtgcgt agggttggtc accacgacgt cggccttggg cacttcggcc atcatgcggc
     2461 ggcgcgccac ttcgcgctgc tggctgcgga tgcgcgcctt gagctgcggg tcgccttcgg
     2521 attccttcat ttcgcgcttc acctcttcga ggctcatgcg caaccccttg tggtgcttcc
     2581 acagcaccag cggcacatcg atcaacgcga tgagagccag cgccgcggcg agcgcgagca
     2641 gcgagccctg caccatgccg ccgaattcgg cgagcccctg accgggttcg agcatgatca
     2701 ggccgagcat ttcctcgcgg tgcagccaca ggaacaggca gccgatcgtg ccgaccagca
     2761 gagccttggc gaccgccttg cccagttcgg ccagcccttc ggcggaaaac gtgcgcttga
     2821 cccacttcag cggactcatg cggccgaggt cgggctggaa ggccctgggg gaataaagcc
     2881 agccgccgat cagaagcgcc gacagaatgg cgatgacaac gaccgtgacc agcagcggca
     2941 ccagaccgag aaaggcgtcg ataccgagcg acaggaaacg ctcgcccacc gccgccgtgc
     3001 tgaaagcgct ttcgcgatct accgtgaggc cggcccgcat caggcggatg gagtagtcga
     3061 cggccgccga tccggccgtc cacgccagtg ccgcaccgga cagcacgacg gcgcacgcgc
     3121 ccagctcgcg cgagcgggcg acctgccctt cctcgcgcgc ctgttccagc cgcctggggg
     3181 tggctggtag ggttttttcc tgatcgcttt ctgcggccat gtgccgtacg gacctctgga
     3241 gacggagcca ttatttccgt ccgacggcaa ggcaaagacg aggattagcg cgggtttaag
     3301 ggccgatatc ggcggatgcg cgcactattg cgagggtctg gcatatcccc gaaccgggta
     3361 ccggcatgcc cgaaatgagt atcgaaatcg acgatgaggc gctggcgaat gcgctgatcc
     3421 gcgtgactgg cgaagtcgcc gaacgcgtca gccgaccggg cccggtcgat caacagcccg
     3481 tcgtccttcg ccccgcctcg ggacgtgggc cgaggcgaag gagcggtgtt tcgactacgc
     3541 cgcgctcaac ctgaaccgcg cgaccacgct ttcgagcatg cgcgccatct cttcgagatt
     3601 ctgcgcggag gaggcgaccg agcccaccgc cgccgcgttc tcttcggaca tgcgcgcgat
     3661 gcgttcgacc gtttgcgcga tctggctgct ggcgctcgac tgttcactca gcgcgtgcga
     3721 gatctcgttg accacgctca gcacttcgct cgcccgcgcc ttcacgtcgc gcatgcactc
     3781 gcccgcgccg cgcgccttgg ccatgccatc ggcaacgcgc acgctccagg tttccatatg
     3841 gctgaccgcc tgaccggtac cgtcctggat ggctttgatc atgtcgccga tttcgcgcgt
     3901 ggattcggtc gtgcgctccg ccagcttgcg cacttcctcg gccaccacgg agaagccgcg
     3961 cccctgttcg cccgcgcgcg ccgcttcgat cgcggcgttg agcgcaagca ggttggtctg
     4021 attggcgatc tcctggatca catgcacgat gcgcgagatc tgcgtggact gcgcgccgag
     4081 cgtctcgatg atctgcgtca tgctttcggc cgacgcggcg atctgcgcca tttccgccgc
     4141 ggcgctctgc accacttcgt cgccgcggtt cgacaactcg cccgaagcgc gcgaaatgga
     4201 cagggcctca ttggagtgtt ccgccacttg gccgatgctc gccgtcatct gctcgatgga
     4261 cgaggccatg gacgaggccg cttcgttctg ctgttcggag cttgcggcaa tctgtacgga
     4321 agacgaggac agcgcagtcg cctgtccgga cagcgcgccg gcgctgtcgc gcaaatgctc
     4381 gatcaatgca cgcagatgat cctgcatgtc ggaaatcgcg gcgagcaggc tgcgatcgtc
     4441 cccctcatgc accgctacgg gcgtagtcag atcgccaccg gcgatgcgcc gcgcgatggc
     4501 aaccgcatcg gcgggatcgc cgcccaattg attcagcagg ccgcgcgtaa tccagctgaa
     4561 caccagccac gcagcgccca tcgccaccag ggtcgacacg atgaagccga tgatcgtcat
     4621 gcgatagttg tctgcggccg attcggaatc ggcggtggcc tgcgccacga agcgctcctc
     4681 catggcgttg cgcaaatcgg tcagcccttt gtcgaccggc gtatcgagtc cgcgcagcat
     4741 cccgtcgatc agaatcgccg aaccgagcgg gttgaagtac tcgtacagct tcagcgcttc
     4801 gtcgtaacgg tcggagacgt cggcgtgctg cttgatcatc gcctcgacgc ggtcgcgggc
     4861 ctcatccgaa gccaggtccc gcagcgagag caattccttg gccgccgcct gccgatgcgt
     4921 gcgcatcgaa tcggtatgct gctggaagac gtcctggtcg gcaccgcgca gaatggcatc
     4981 cttcaactcc agcgtctgca tgcggaaatg ggcccgtgcc gcctccgcgt tgcgcagcat
     5041 caccagcaca cgatcctgct cggacaacac gtcgttcagt ttgtcgtgct gcagccagac
     5101 gccgaaaatg ccgacgcccg cgccgccgat cagcatcgac atcagcagtg cgcccagcag
     5161 cttgaagcgc gttccgattc tcatattctc gagcatgatg tgctccttct tcctatccct
     5221 taaagaagcc aaggttgttt gttcgattga agccggcccc ggtcgccgaa gcgaacccgt
     5281 ccttacagcg acgcgcactc gtcatccccg acattgccgg attccctccg ccattcatcg
     5341 cacgccgtcc agcaactcgt gcaactcgag cacgagcacc agcgaacctt cgccggaaat
     5401 cgttgcgccc gccacgccgc gcggcttcac gccggcgagc ggtttgatca tcacttcgtc
     5461 ctgtcccatc aatccgtcca cgcccagcac catcgtgcga tcgcccaccg tcgcaaccac
     5521 cccgagcggc gcgagaccgt ctcgcggccg attgaggaga tgcgaaagct cgacgaaagg
     5581 cagcacctcg ccgcgcatca ggatgtgcgg cttgccggag acgtattcga tgacctcgtc
     5641 ggacagctcg atgatttcgc gcaccgcagc caggggcacg gcgaaaggct gcatccccag
     5701 acgcaggttg aggaccggca ggatcgccaa cgtcagcggc agcacgatgc tgacggtcgt
     5761 gcctttgccg ggcacggact ccagatcgat acgtcccttg agtcggtgaa tgttggtttt
     5821 caccacgtcc atacccacgc cgcgcccgga caggttgctg gcctgttcct tggtggaaaa
     5881 acccgccgcg aaaatgagct gcaggctctg tcgatcgtcg agctgcaccg cttcctcggg
     5941 cgagatgacg cccttctcca ccgccttggc gcggatgact tccggccgca tgcctttgcc
     6001 gtcgtcggcg atttcgataa tgatctggtc gccggtctgc cgcgcggcga gccgcacgat
     6061 gctcttggcg ggcttgccct gtgccacgcg ctcggccggc atttccacac catggtcgac
     6121 ggcgttgcgc accaggtgga ccaatgggtc gttgagttct tcgaggatgg tcttgtcgac
     6181 ttcggtatcc tcgcccacca gtaccagctc gatttccttg ccgagttgcc gcgccagatc
     6241 gcgcgtcagg cgcacgtatt tctggaacac acggccgacc ggctgcatgc gcgccttcat
     6301 caccgagttc tgtaggtcgg aaacgagcat gtcgagctcg ttcaccgcgt cgtccagcgc
     6361 cttcagcgtt tccatctcgc cgccgacgcg cagcagatcg cggcggatgc aggcgagccg
     6421 gttcttggtg agtccgattt cgcccgacag attgagaatc tggtcgaagc gctgcgtatc
     6481 gatgcgcagc gtgttttcct tctgcgcgcc gccgcctttg gccgcaccac cgttcttctg
     6541 tgcgccattg cctttcggtg ccggcgcttt gggcgcgggt gcttcgacgg gcttcgtctc
     6601 tttggctgcc gccgcaggtt gcgcagcgac agccttgccg ctgacggccg catagagcgc
     6661 ggcccaatcg ggctcgccgg caacagccgg caccgcatcg gcgttcgatg cgaccgttgc
     6721 ggaaacctca gccgctgcgg gagcaggcgc ctcgggcagc gactcgccct gtgcgagcgc
     6781 ttcgagttgc gcgatgagtt gttcgggcgc cggatcgggc tgcaccagat tcgccatctc
     6841 gccgaacatg cgccgcactt cgccggtcgc cgcgaggatg taatccagca cgtccggtgt
     6901 gagacgcagt ttgccgctgc gcagccggtc gagcagcgct tccgcgcgat ggcagatcgt
     6961 caccagcgcg gtcgcctcca gaaaacccgc accgcccttg atggtgtgaa agccacggaa
     7021 cacggcgttc agcagatcga gatcgtccgc gcgcccttcc agttcgacca gttgcagatc
     7081 gacgtcgtcc agcagttccc ccgcttcgct gaggaactcc agcaacagat cctgcatgtc
     7141 ggtgaaggcg ttcatgctga aacagccatg gctacagagg cacagagcac aatctgcctg
     7201 gagattttcg tcaccaggtc agccgcaagg atgggcacca cgaccgccgt cattcccgcg
     7261 caagcgggaa tccagtgcgc ggtccatgct cgaccacaac ggcgtacgta cgggtgcttc
     7321 atcgctcttc tctgtgcctc tgtgtctctg tggttcagtc gttaaaaccc tggattccgg
     7381 gtcgcgcagc ttcgctgctt gcccggaatg tcgagacgaa cgctctcggc tctcggctgc
     7441 atttcaaaaa cccaggctct ccagcagttc gtcgacctgc tgctggttgg cgacgacgtc
     7501 gtcgcgttcg tcggcgttca ttgccggacc ggtgagccat tcgctctcgg acgccggccg
     7561 tttttccggc ggcgtggttt cgatgagcag cttgaccagt tgcgactcca ggttctgcgc
     7621 caccttgacg atgcgctgga tgacctggcc ggtgaggtcg tggaaatcct gcgccatcat
     7681 gatttcggtg aactgctcgt tcgtgcgcgc ggtgtggtcg tcgagcgact tgagaaagcc
     7741 gcgcgtctcc accgccgtgt gcttgaactc ctccagttcc agttcgccgt tgaagacgcg
     7801 ctcccactgc gccgccagac ggctggcttc ggaccgcact tcgttctgat attgctgccc
     7861 ctgctccacg gccgtcagcg cgcgctccgc cgccttgccg gtgagatcgg cgatgtagct
     7921 caggcggtcg cgcgcatcgg gcaggctgct caccgcgtcc tcgacggcct tgtcgtaacc
     7981 cagttcgtgc agcgcatcgt gcagattgcg cgtcagcgcg ccgatgcgtt gaaacacttc
     8041 ctgcgggggc ggcggttcgg cggattgcgc ctgcccgacg acgatgtcgg cagccggctc
     8101 ttcgttcact tctggtgcgg gtgcatcttc ctttgtgaac ggctccgctt cgctgcgctc
     8161 ggccgccacc cggtcgaaca gcgcttcgag gtcttcctcc tcggccggcg ccgcttgctc
     8221 ggccgccgat tgcgcggcta cctgatcgaa cagcgcttcc agatcttcct cttccgccgc
     8281 gggcgccggc tgctgcgctt cggctgcgcg ctgcgccgcg atttcgtcga acagggcttc
     8341 gaggtcttcg gtctcggttc cggacatgtc taaactccca tctgccgcgc tacgccgcct
     8401 gcttctgcaa aatcttgttg agtttctctt ccagcgtcgc cttggtgaac ggcttcacga
     8461 tgtagccgga ggcgcccagt tgcgccgcgg tgacgatgtc ttccttcttc gcctcggcgg
     8521 tgaccagcag caccggcagc accttcagat tttcatcggc gcgaatctga cgcagcagct
     8581 cgaagccgtt catgttcggc atgttgagat cggagacgac gaaatcgaag ccgccgctct
     8641 tcagcttggc cagcgccacc acgccgtcct cggcttcgtc ggcattgacg tagcccaact
     8701 ccttgagcag gttgcgcacg atacggcgca tggtggagaa atcgtcgacg atcagaaact
     8761 tcagttcggt actcatgctt gactcccttg ctcacgtccg gaacgcagca ttccggcgat
     8821 ggtgccggca agttcttgaa agtgaaattt ggcgacgtag gcatcgacgc cgacctgctg
     8881 tccgagcttc ttgttcgcca ccgaggacag cgacgaatgc atgacgacgg gaatgccgtt
     8941 gaaacgcgga tcctgtttga tgtggcgtgt gaggacatag ccgtccattt caggcatttc
     9001 cgcatccacc agcaccacgc ccagccgctc gctgagcggc acgccctcga tctgcgcctg
     9061 cgcggcgagc aactgcaacc gctccagcgc ctcgcggccg ttgatggcct gctggtaggt
     9121 caggcccatc tgatcgagca cctgcgtgac tttcttgcgc gcgatggccg agtcgtcggc
     9181 gaagaacacc atgcgcgacg aatcgctgac cggcgcgtcg agcggcggca cttcctcctc
     9241 gcccatcacg tcgacgagga tctgctcgac atcgaggatc gacaccaggc ggccgcccgc
     9301 cagttcggtg atggccgtga ccagtcccgc gccgccggtg acggtggatt gcggcgactt
     9361 cacctgctcc caatccacac gcacgatgcg atcgacctcg cccaccagaa acgcctgcgt
     9421 gctggacgag aactcggtga tgatcagctt gcccccttcg gccgtctgct ccatgcccag
     9481 acaacgcgcc aggccgatga ccgggatgat tgcgccgcgc aggctgatga tcccttccac
     9541 gccgtgcggc atgttcggcg tgggcgtgat gggcttcatc tcgcacacct cgcgcacctt
     9601 gaatacgttg atgccgaagg tctcgcggcc gcccagcgag aacagcagca gttccatgcg
     9661 attggaaccg gccagtcgcg ccttctgctc cacgacgctc agcagtcgat cgtcggtcgt
     9721 catcatcttc cttctcccag gcctagcgcc cgccgccggc cggatcgatc tccggtgtga
     9781 cggcatggat catgtcgttc gccagctcgg cgtcgggtac ctgcaccgcg atgccttcgc
     9841 tggtgatcat ttcttcggtt ttcttgttca gcaccacgat gctgatgcgg cggttgatct
     9901 cgtcgaacgg gtccgcggtg ttgagcggaa tcgacgaggc cagtccgacg acgcggatga
     9961 tcttgtcttc ggcgataccg cccgcgacca gttcccgccg cgatgcgttg gcgcggtcgg
    10021 cagacagctc ccagttgctg taaccgcgat cgccggtgaa atagggtttg gcgtccgtat
    10081 ggccggcgag ggagatcttg ttctccacgt cgttcagcag cccgcccagt tcgcgcagga
    10141 tttccttcat ccagggatgc accaccgagc gcccgttatc gaacatcggg cggttctggt
    10201 cgtcgacgat ctggatgcgc aagccttcgg aagtgatgtc gagcttgatc tggttgcggt
    10261 aggccgagaa acgcgggttg gagtcgaaga tcttttccac gcgctccttc aactgcttga
    10321 gtttgacggc ctcgtcgcgt gcgatctccg cgcgcgccgc ctgcaggttg atgatcttgg
    10381 tgcggccgat atcgcccatc ttcacctggc cgtgcgtacg cgtcaggtcg ttgccgccgc
    10441 cctggatgac cgaggagctg tcgcccgaac ccgagccgcc cgagagcgcc accttgagcg
    10501 gcgtgttgaa gtagtcctga atccccttca gatcgccctg ggtggtggaa ccgagcagcc
    10561 acatcagcag aaagaaggcc atcatggcgg tgacgaagtc ggcataggcg accttccaca
    10621 tgccgctgcc gtggttcggc tggaccttct tgatccgctt gatgacgatg ggctggagcg
    10681 ggtctcgttt catcgccgac tcaacgcttc aggctcgttt tgacatgctg ttccagctcc
    10741 atgaagctgg gacgctcggt ggagtacagc accttgcggc cgaactcgac cgccagcgcc
    10801 ggggcataac cgttcaggct ggccagcagc gtcaccttca cgcattcgag catcttgttc
    10861 gactcggcga cgccctgctc gatcttgctc gccagcggcc cgacgaagcc gtaggagagc
    10921 aggatgccga ggaaggtgcc gaccagcgca tggccgatca gcttgcccag ttccgccggc
    10981 ggcagaccga ccgagcccat ggtgtgcacc acacccatca ccgcggcgac aatgccgaac
    11041 gccggcagac cgtcgccgac gcgctggatc gcgtgcgccg gtacgatcgc ctcgttgtga
    11101 tgggtttcga tttcgttatc catgagattt tcgatttcca tcgggttcag gttgccgctc
    11161 accatcagcc gcagatagtc cgtaatgaac tcgatgagat gcgcatcctt ggcgatcgcc
    11221 ggatacttgg tgaacatcgg actgtcctgc ggcgcatcga cgtcgcgctc gatggacatc
    11281 aggccttcct tgcgcacctt ggtgagaatc tcgtacagca ggctcatcag ctccatatag
    11341 agcgccttgg tgtagcggga gcttttcaac accgaaggca gcgtcttgaa tgtggccttg
    11401 agcaccttgc cgttgttgcc caccacgaaa gcgccaaagg cggcgccggc gatgatcagc
    11461 agttccagcg gcgccagcat cgacgccaga tggccgcccg cgagggcaaa gcctccgaac
    11521 accgatccca agacgatgat gtaaccaacg atcaccaaca tgtacgcgct tcccctgtgt
    11581 cacccctaag gcgcgtcgcc gctcgcgccg gcctcggcag ccgaccggcg cagacgctcc
    11641 ggtacaacat ctttgtcggc ataaagggcg ttttcttgag cacagcgggt ccatgacgtg
    11701 gaagagtaac ggccggaacg tcggggacat ggcgcgaata gcgccgggtt tggcgggtat
    11761 ttcgggcttc ggcgggctac acatatatat atgtgatgtg agtcaagcgc cgagagccgg
    11821 gagtcgagag caaaagcccg tcaaaacctt gaaccacaga ggacacagag ggctcagaga
    11881 aaaccggctt ttaaagcctt tctctgtgtg ctctgcgccg tctgtgtttc aagattcagg
    11941 gtttg
//
LOCUS       DATGOU010000030         4142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_65486, whole genome shotgun sequence.
ACCESSION   DATGOU010000030 DATGOU010000000
VERSION     DATGOU010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65486"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VKZ48_01760"
     CDS             complement(<1..993)
                     /locus_tag="VKZ48_01760"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLU76612.1"
                     /translation="MAHDIEAVVEQAENMLGRPEMPVSRHIFVTGANGFIGSALCRFL
                     AAQGAKVTALMRRPPAQALPEGVEAMLLPSYDALEPRHLQGHEAVIHLAGHAHAAHMN
                     PAQRGRAFAEDVRLARLAAGCAATAGAKRFVFVSTIKVLGEQSGALPFDHRSIPAPAD
                     AYARARLAGEQAVREVAEAAGMEWIVVRPVLVVGEGARGNLDALLRWISAGRPLPFAG
                     MNNRRSLIGLEDLCELLACCAVHPHAAGRTFIASGDETVSTTDICVALAEGLGVRPRL
                     FRVPRWLWSMARAFPPTRGLEQRLAGSLQADNTETRRLLGWQPRMRARDCLFRLA"
     gene            complement(998..1879)
                     /locus_tag="VKZ48_01765"
     CDS             complement(998..1879)
                     /locus_tag="VKZ48_01765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLU76613.1"
                     /translation="MDANSREAVRVSIVVPVFNQWHLVPELLRCLEMQTFDHDAFEVL
                     LVDNGSEYIPPPTGEPRFVRRLVCSTPGSYAARNLGIAQARGAVVAFTDADCRPHPQW
                     LQAALGRMQGSRMLLAGAIRIVPRDPGNPNRYEVYDMMLGMPQARYVRRGYAITANLF
                     VPRAVLNELGGFDARRFSGGDAAFCRRARDAHIALEYVESAVVEHPARDSWAALATKV
                     RRIKGGQLAAGSLRQKALYAVRAFLPPVRAWWRVLRASSFSWRHRIGSLFVQARLWLV
                     EMAEVLRLLLGRQPERK"
     gene            complement(1885..>4142)
                     /locus_tag="VKZ48_01770"
     CDS             complement(1885..>4142)
                     /locus_tag="VKZ48_01770"
                     /inference="COORDINATES: protein motif:HMM:NF017263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HLU76614.1"
                     /translation="GAYWHRAAHARMSMALNGFFRFLRWVARRAGMLHPALQGVRRSS
                     WIGRHLPQSLRFALGIGVLADRGYRFPYERGKPPHGDVARMQREYVERGLHEMPDTFV
                     LYRIIGNDLYPRHRKGQSRDNLRFILENEPDFEHCEKRFIVNRIVDADEERQIIGMLE
                     RAGRPYQRIPFDWNEYGDVGWDADGIPAEYSPVSESFARLPAPYRNRALMRLYRYKNN
                     YAMNNNGARNVALRDGRNRAKWVLPWDGNCFITAAAWEEIVAGVKAHPEVPYHIVPMA
                     RINDNVELLQAGFRPSALEEPQVLFRKDAALEFDIEHPYGRRPKVELLWRLGVPDGWD
                     RWPIEPWDLPVADYAPDAGKWMRSGWVVRLASGRPELEKGATSRNDRGIARIEAITTM
                     LDHLDEQVLERRLDAQAPVFVRSDFAQRADPALVEALRLAAEEALDCGTLRVPGLPCI
                     PRDRTRASGTRMYEPLGEQYDRTRLQRLFDDTWVLGLAFQSTREARFAEHAAKLVRTW
                     FLDEATRITPHLNYAQARIGWGRNKGRGAGIIEMKDLYYFLDAIRLLQLSGALTEAEI
                     ESLKNWFREYLQWLLKDAQGRRERTRRNNLGTYYDLQVTAIAAWLGEVLVLRDALRDA
                     RLRVSLQFDADGRQPEELKGTITAHYCCFNLQGWVNLAELAERTLGVDLWSEDCKGRS
                     LRKALEWTLQYRNREWPFPQQEPFDAERFLPLQYAYERRFGERSGIDFAKVKPVFHPH
                     DGIRPFWQLQE"
BASE COUNT          772 a         1431 c         1243 g          696 t
ORIGIN      
        1 agcaaggcgg aacaggcagt cgcgagcacg catgcgcggc tgccatccca gcaggcgacg
       61 cgtctcggtg ttatccgcct gcagcgaacc ggcgagacgc tgctccagcc cacgcgtcgg
      121 cgggaaggcc cgcgccatgg accacaacca gcgtggcacg cggaacaacc ttggacgtac
      181 tcccagacct tcggcaagcg caacgcaaat gtctgttgtc gacactgtct cgtcgccgga
      241 agcgatgaat gtcctgcctg ccgcgtgcgg atgaacggcg cagcaggcca gcagttcgca
      301 aagatcctcc aggccgatca gcgaacggcg gttgttcatg ccggcgaatg gcagcggccg
      361 gccggccgag atccatcgca acagcgcatc gagattgccg cgcgcgccct cgcccaccac
      421 cagcaccggc cgcaccacga tccactccat gccagcggct tccgccacct cgcgcaccgc
      481 ctgctcaccg gcaagcctgg cgcgggcata ggcgtccgcc ggcgccggaa tgcttcgatg
      541 atcgaaaggc agcgccccgc tctgctcgcc cagcaccttg atggtgctga cgaacacgaa
      601 gcgcttcgcg cctgcggttg ccgcacaccc ggccgcaagc cgcgcaaggc gcacgtcctc
      661 cgcgaaggcg cgcccccttt gcgcggggtt catgtgcgcc gcatgcgcgt gccccgcaag
      721 atggatgacg gcctcatgcc cctgcaggtg ccgcggctcc agggcatcgt acgagggcag
      781 caacatcgcc tcgacccctt caggcaaggc ctgcgccggc gggcggcgca tcagcgcagt
      841 caccttcgcg ccctgcgcgg cgaggaagcg gcacagcgcc gagccgatga agccgttggc
      901 accggtcaca aaaatgtgtc gcgataccgg catctccggc ctgccgagca tgttttccgc
      961 ctgttcgacc acggcctcga tgtcatgtgc catcaggtta ttttctctcc ggctggcggc
     1021 cgagcagcag gcgcaggact tccgccatct cgaccagcca cagtcgcgcc tggacgaaca
     1081 ggctgccgat tcggtgcctc caggaaaacg acgaggcgcg caggactcgc caccatgcgc
     1141 gcaccggtgg aaggaaggcg cgcaccgcgt acagggcttt ctgccgtaac gatcctgcgg
     1201 cgagctgtcc gcccttgatt cgccgcacct tggtagccaa agccgcccag ctgtctctcg
     1261 cggggtgctc taccacggcg gactccacgt attcgagggc gatgtgcgca tccctggcac
     1321 gccggcagaa cgccgcgtcg ccgcccgaga atcgccgcgc gtcgaaaccg ccaagttcgt
     1381 tcagcactgc ccgcggcacg aacaggttgg cagtaatcgc atacccacgg cgcacgtacc
     1441 gcgcctgagg catgccgagc atcatgtcgt acacctcgta gcggttcggg ttgcctgggt
     1501 cacgcggcac gatgcgaatc gcgccggcca acagcatgcg tgagccctgc atcctcccaa
     1561 gtgccgcctg cagccattga ggatgcggcc ggcagtccgc atccgtgaac gcgactaccg
     1621 ccccgcgcgc ctgtgcgatg ccaagatttc tcgctgcata ggagccgggc gtgctgcata
     1681 ccaaccgtcg tacgaatcgc ggctccccag tcggtggagg aatgtactcg gagccattgt
     1741 ccacgagcag cacttcgaag gcgtcatggt cgaaggtctg catctccagg cagcgcagaa
     1801 gctccggcac gagatgccac tggttgaaga ccggtacgac aatcgagacc cgcacggcct
     1861 ccctggaatt cgcgtccatc ggcttcactc ctggagttgc cagaaagggc gaatgccgtc
     1921 atgcggatga aacaccggtt tcaccttcgc gaaatcgatg ccggagcgct cgccgaaacg
     1981 ccgctcgtag gcgtactgca gcgggaggaa gcgctcggca tcgaaaggct cctgctgcgg
     2041 gaatggccat tccctgttgc ggtactgcaa tgtccactcc agagccttgc gcaagctgcg
     2101 tcccttgcaa tcctcgctcc acaggtcgac gcccagcgtg cgctccgcga gttccgcaag
     2161 attcacccac ccctgcaaat tgaagcagca gtagtgcgcg gtgatcgttc ctttcagctc
     2221 ttcgggctgg cggccatcag cgtcgaattg cagcgagaca cgcaaccgag catctcgcaa
     2281 cgcgtcgcgc aacaccagca cctcgcctag ccaggctgct atggccgtca cctgcaggtc
     2341 gtagtaggtg ccgaggttgt tccggcgagt acgctcgcgc cggccctgtg catccttcag
     2401 cagccactgg agatactcgc gaaaccagtt cttgagcgat tcgatttccg cctcggtcaa
     2461 cgccccgctc agctgcagca ggcggatcgc gtcgaggaag taatacaggt ccttcatctc
     2521 gatgatgccc gcaccccgcc ccttgtttcg gccccagccg atgcgcgcct gggcataatt
     2581 caggtgcggc gtgatgcgag tcgcttcgtc aaggaaccag gtacgcacca atttcgccgc
     2641 atgctcggcg aaacgcgcct cacgcgtcga ctggaatgca agcccaagca cccaagtgtc
     2701 atcgaaaaga cgctgcaacc gcgtgcggtc gtattgctcg ccgagcggct cgtacatgcg
     2761 cgtgcctgac gcacgcgtcc tatcgcgcgg aatgcaaggc aggccgggaa cgcggagcgt
     2821 cccgcaatcg agggcctctt ccgccgcaag ccgcagggcc tcaaccagcg caggatcggc
     2881 acgctgcgcg aaatcgctgc gaacgaatac cggcgcctgc gcatcaagcc ttctctccag
     2941 aacctgctca tcgagatgat cgagcatcgt ggtaatcgcc tcgatgcgcg cgatgccgcg
     3001 atcattgcga cttgtcgcac ccttctccag ctcgggccgc ccggaggcaa gccgcactac
     3061 ccagccgctg cgcatccact tgcccgcgtc gggggcgtag tccgcaaccg gcaaatccca
     3121 gggttcgatc ggccagcgat cccagccgtc gggaacgccg agtcgccaca acagctccac
     3181 cttcggccgc cgcccgtacg gatgctcaat gtcgaattcg agcgcagcat cctttcgaaa
     3241 cagcacctgc ggttcttcca gcgccgacgg gcgaaagccc gcctgcagca gttccacgtt
     3301 atcgttgatc cgcgccatcg gtacgatgtg atacggcact tcgggatgtg ccttcactcc
     3361 ggcgacgatt tcctcccatg ccgcggccgt gatgaagcaa ttgccgtccc acggcagcac
     3421 ccacttggcg cgattgcgcc catcacgcaa tgcgacattg cgcgcgccgt tgttgttcat
     3481 tgcgtaattg ttcttgtacc ggtacaggcg catgagcgcg cgattgcgat acggcgcggg
     3541 caagcgggcg aacgactcac tgacgggcga atactctgcc ggaatgccat cggcatccca
     3601 gccaacgtcg ccgtattcgt tccagtcgaa ggggatgcgc tggtagggac gtccggcacg
     3661 ctccagcatt ccgatgatct ggcgctcctc gtcagcgtcg acgatacgat taacgataaa
     3721 gcgcttctcg cagtgctcga aatcgggctc gttctcaagg ataaagcgca ggttgtcgcg
     3781 gctctgtccc ttgcgatgac gcggatacag atcgttgccg atgatgcgat agagcacgaa
     3841 ggtatcaggc atctcgtgca ggccccgctc gacgtattcg cgctgcatgc gggcgacgtc
     3901 gccgtgcggc ggcttgcccc gctcgtacgg gaagcggtac ccccggtcgg caagcacgcc
     3961 gatgcctagt gcgaaccgca gcgattgcgg cagatgccgg ccaatccagg aactcctccg
     4021 caccccctgg agtgcgggat ggagcatccc cgcccttctg gccacccacc tgagaaaccg
     4081 gaagaaaccg ttcaaggcca tcgacatcct cgcatgggct gcgcgatgcc agtacgcacc
     4141 at
//
LOCUS       DATGOU010000031         2607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_4956610, whole genome shotgun sequence.
ACCESSION   DATGOU010000031 DATGOU010000000
VERSION     DATGOU010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4956610"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VKZ48_01775"
     CDS             <1..424
                     /locus_tag="VKZ48_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HLU76615.1"
                     /translation="DVLPQETLAYTLRYKLGLTGTKIGCNRGECGACTLLIDDVAHYS
                     CSTLTHSVKARSVVTVEGLQASDGTLHPVQQAFIDELAPQCGFCTPGQVMSAVALLKV
                     NPQPSREEARQALSGNLCRCGAYDHYLNGVMRAAGTRA"
     gene            421..>2607
                     /locus_tag="VKZ48_01780"
     CDS             421..>2607
                     /locus_tag="VKZ48_01780"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HLU76616.1"
                     /translation="MNNYRHIGKDFVPPDVVGKVTGKARYAEDFRADGMVFARLYTSP
                     MAHGRVTSIDTSAVDEMDGVIGILTADDLTPVEPPNAPMLTNTPVYIGEPILAVAAID
                     EKTAEDAISRIRVHIEPLPFVVDPLDSLVEGGPDAREEGNVYLRSREGSGFRSVKWTA
                     EQIDEFRAGKEPTGESVNEWSYGDLEKGFADAEVIVEESFVTTGYAHMSMEPRTAMAY
                     WENGTCYVYGSAQSQSFMIPGLASLLEVEQKNVVLVSENTGGGFGSKIGAYPMMALPA
                     WFSKKLKRPVQLRITREQEYYVGSARVGFQGWIKVGFAANGRVTATDLFIVEDLGPNA
                     TGGDGSSAGGAVSIVYDPEAMRFRGVPVLTNTTPRGAQRGPGQNQIAAVMPAIMDEAA
                     RRLGMDPLQIRLANAAHNGTTVEEDQGPITSAYMKEALEMGAEMFGWEKKRGQPRRKG
                     NKVIGLGIGQGYHSAGSDGFDGLVRITPDGKIHLHSGVGNLGTYSYASTTRSAAEVLK
                     CDWNNCVIHRGTTEAHLPWSSYQAGSNTTFTHQRANFVAATDAVNKIKEIAAATFGGN
                     PADYDIADERVFRISNPAQAMTYAAVAERAIELGGAYSGASWPDDIHEITQRAVQGIA
                     GTGLIGVGKDTLPLNGIVPGLAVAFAEIELDLETGKYVILDYVGIAECGTVVHPQGLA
                     AQMKGGAVWGMGMAALERHVYDPQNGLPANVGYHQCGIPTYLDTPSV"
BASE COUNT          528 a          823 c          843 g          413 t
ORIGIN      
        1 cgatgtgctg ccgcaggaaa cgctcgccta cacgctgcgt tacaagctgg gactgacggg
       61 cacaaagatc ggctgcaacc gcggcgaatg cggtgcctgc acgctcctga tcgacgatgt
      121 tgctcattat tcctgttcga ctctcacgca cagcgtcaag gcacgcagtg tcgtaaccgt
      181 cgagggcctg caggccagcg acggaacgct gcatcccgta cagcaggcgt ttattgatga
      241 actcgctccg cagtgtggct tctgcacgcc aggacaggtg atgagcgccg tggcactcct
      301 gaaagtgaac ccgcagccga gccgcgagga agcgcgccag gcactttcag gcaacctttg
      361 ccgctgcggc gcctatgacc attacctcaa tggcgtgatg cgggccgcgg ggacacgcgc
      421 atgaacaact accgccacat cggcaaggac ttcgtgccac cggacgtcgt gggcaaagtc
      481 acgggcaaag cccgttacgc ggaagatttt cgcgcggacg gcatggtgtt tgcgcgcctc
      541 tacacgagcc cgatggccca cggccgcgtc accagtatcg atacctccgc cgtcgacgag
      601 atggacgggg tcatcggcat cctgacggcc gatgatctga caccggtgga gccgccgaat
      661 gcgccgatgc tgacgaacac gccggtctac atcggcgagc ccatcctcgc ggtcgcggcc
      721 atcgacgaaa aaaccgcgga ggacgcgatc tcgcgcatcc gcgttcacat cgagccgctg
      781 cccttcgtag tcgacccgct ggacagcctg gtagagggcg gtccggatgc ccgcgaggaa
      841 ggcaacgtgt acctgcgttc acgcgaaggc tcgggcttcc gcagcgtgaa atggacggcg
      901 gaacagatcg atgaattccg cgcaggcaag gagccgaccg gcgagtccgt caacgagtgg
      961 agctacggcg atctcgagaa gggcttcgcc gatgcggagg tcatcgtcga ggaatccttc
     1021 gtcaccaccg gctatgcgca catgagcatg gagccgcgca ccgcgatggc gtactgggag
     1081 aacggcacct gttacgtcta cggatccgcg cagagccaga gcttcatgat tcccgggctt
     1141 gcgagcctgc tggaggtgga gcagaagaac gtcgtgctgg tcagtgaaaa taccggcggc
     1201 ggcttcggct cgaaaatcgg cgcgtacccg atgatggcct tgcctgcgtg gttttcgaaa
     1261 aagctcaaac gccctgtgca gcttcgcatt acgcgcgagc aggaatacta cgtcggctcg
     1321 gcgcgcgtcg gtttccaggg ctggatcaag gtcggcttcg ccgcgaatgg ccgggtgacc
     1381 gcaaccgacc tgttcatcgt cgaggactta ggtcccaacg ccaccggcgg tgacggctca
     1441 tcggccggcg gcgctgtctc gatcgtctat gacccggagg ctatgcgttt tcgcggcgtg
     1501 ccggtgctga cgaacaccac gccgcgaggc gcgcagcgcg ggccggggca gaaccagatc
     1561 gcggcggtga tgcctgcaat catggacgag gccgcacgcc ggctcgggat ggaccccctg
     1621 cagattcgcc ttgcgaacgc cgcacataac ggcaccaccg tggaagaaga ccagggcccg
     1681 atcaccagcg catacatgaa agaagcgctc gagatgggcg ccgagatgtt cggctgggaa
     1741 aagaaacgcg gccagccccg acgcaaaggc aacaaggtga tcggcttagg catcggccag
     1801 ggctaccatt ccgccgggtc ggacggcttc gacgggctcg tgcgcataac gcccgacggc
     1861 aagatccacc tgcatagcgg cgtcggcaac ctcggcacgt attcctatgc atcgacgacg
     1921 cgcagcgccg ccgaagtgtt gaagtgcgac tggaacaact gcgtcatcca tcgcggcacc
     1981 acggaggcgc acctgccctg gagttcgtac caggccggca gcaacaccac gttcacgcac
     2041 cagcgcgcga acttcgtggc ggctaccgat gctgtcaaca agatcaagga aatcgccgct
     2101 gcaacatttg gcggcaaccc ggccgactac gacatcgcgg acgagcgcgt gttccgcatc
     2161 agcaatccgg cacaggccat gacctacgct gcggttgcag agcgcgcgat cgaacttggc
     2221 ggcgcctaca gcggcgcaag ctggccggat gacatccacg agattacaca gcgcgccgtg
     2281 caaggcatcg ccggcaccgg actcatcgga gtcggcaagg atacgctgcc gctaaacggt
     2341 atcgttccgg gcctcgcggt tgcattcgca gagatcgagc tggacctgga aaccggcaag
     2401 tacgtaatcc tcgactacgt cggcatcgcg gagtgcggca ccgtcgtgca cccgcagggc
     2461 ctggctgcgc agatgaaagg cggggccgta tggggcatgg gcatggcagc gctggagcgt
     2521 cacgtttacg acccgcagaa cgggctgccg gcgaatgttg gctaccacca gtgcggcatc
     2581 ccgacgtacc tggatacgcc gagcgtc
//
LOCUS       DATGOU010000032        16349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_960775, whole genome shotgun sequence.
ACCESSION   DATGOU010000032 DATGOU010000000
VERSION     DATGOU010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16349
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960775"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="VKZ48_01785"
     CDS             complement(<1..1217)
                     /locus_tag="VKZ48_01785"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HLU76617.1"
                     /translation="MSAQKTKVAPVTAPAEQEICAEVLIEKYAKGGERTLEEVRRRVA
                     RALAAVEPEDKRAYWEQKFLEAQEMGFVPAGRINSAAGTTLSATLINCFVQPVGDSVT
                     EVVDGRPGIYTALAEAAETMRRGGGVGYDFSSIRPVGALVRGTQSRASGPVSYMRVFD
                     RSCETVESAGARRGAQMGVLRCDHPDIEAFIHAKDEGDLTNFNISVGVTDPFMEAVEK
                     DDDVELVHKAEPAQDVKDAGAYQREDGLWVYRKVRARDLWDQIMRSTYNHAEPGVLFI
                     DRMNKDNNLYYCETIEATNPCAEQPLPPYGCCCLGSINLTKFVTNPFTDKAAFDFAAF
                     GKVVDVATRMLDNVLDATHWPLEQQHEEAKAKRRVGLGFTGLGDTLIMLRLRYDTEEA
                     REMARKISESMRDR"
     gene            1590..3167
                     /locus_tag="VKZ48_01790"
     CDS             1590..3167
                     /locus_tag="VKZ48_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HLU76618.1"
                     /translation="MEPPIELTIAAFNDLHGHLEAPRGEVGGVAAMAARIDALRRERP
                     HTVVVSAGDLIGASPLLSGLFRDEPTIEAMNRLGLDFAAVGNHEFDAGAAELRRLQTG
                     GCHASGVRTCNGAQAGTSVPFEGARFRFLAANVVDRRTGETLFPPYAVRRFDGVPVAF
                     IGLTLRGTAGIVRAAGIAGLRFEDEARTVNRLVRRLRAEGVEAIVVLLHEGGRHSGHP
                     DECVDFDGPIVDIVARLDDAVDLVVSGHTHQAYHCRLPNAAGRAIPVTGAGSYGRYLT
                     RIDLRLDAASGDVRGVEVHNGAVRRDGPQHPAVAQLVRDYAALAEPFAQRVVGRIAAD
                     FTRDPDAAGQSTLGQLTADAQLAVTRDAGAVAAFMNMGGLRADLRARSSGEVTYEDLY
                     AVHPFGNALVTLTLSGAQIKTMLEQQFGCPRERSRPRPLQVSSGFEYRWNPAAAPCAR
                     VVAESIRVQGAPLAPTQHYRITVNDFLADGGDGLRVLREGTERTGGMLDRDALASYLQ
                     QHGPVRPAPTQRVRLVH"
     gene            3182..3751
                     /gene="msrA"
                     /locus_tag="VKZ48_01795"
     CDS             3182..3751
                     /gene="msrA"
                     /locus_tag="VKZ48_01795"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HLU76619.1"
                     /translation="MSKRISPDDIPTGKEVATLGGGCFWCLEAVYDELKGVDSVESGY
                     MGGEVDNPTYEQVCDGSTGHAEVVRITFDPHVTTYQDILQVFFVIHDPTTLNRQGNDV
                     GTQYRSAIFYHTETQKEVAELAIARLGEAQVWPRPIVTEVVPAGDFWMAEDYHQEYYR
                     RNPNQSYCNFVVEPKVQKFRKAFMEKLKK"
     gene            complement(3813..4733)
                     /locus_tag="VKZ48_01800"
     CDS             complement(3813..4733)
                     /locus_tag="VKZ48_01800"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HLU76620.1"
                     /translation="MSTREALLDPDAIETGKVLPFSVYAADGRLLLAQGQIVESERMC
                     ELLRTTGVSCSRTGNPQDAANALKEEEERNCPILRLSRAYAQTARRSMSGLHMAPSDS
                     QEGYTTWVIGMHQRKSLILSHPVRPDKSLVPVLDGEQWVFRTFHATSVFRFTATVLKV
                     SFEPFPHLHIDMPRLVDQRSVRKSPRAAMALVSEIKTATRKERCLVVDLSAQGARVAT
                     PNHLILAKGDKAQIELKLRVGEREYKLALDACIASTYGASDAQHPLASFYGLSFENLS
                     DRDLLVLHGCVYERLASELDSLWQALSIQT"
     gene            5317..6411
                     /locus_tag="VKZ48_01805"
     CDS             5317..6411
                     /locus_tag="VKZ48_01805"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HLU76621.1"
                     /translation="MFINVLRSVFAGVTLLCAVQAAHADRLVLKNGDVLTGTVLHKSG
                     DSLSFSTPYAGVISVSWAAVRTLTTDKAVNVMLNDATDYDGRLDAMGDGRAVLTLSKD
                     PVTGAADEEHEGEPTLAQEEAAPRAELDLAKVLYINPTPEESGEGYRITGRANVAYSN
                     ASGNTENEQLHGNAEMVLRAKMYRYVLGGEATRASDNGNVSAHNARLYTNYDWFFRPK
                     QFAYAAASVERDRFKDIHLRSVVGGGYGYQWIETDVTKLSLRGGLDYVTIDHYDAENE
                     HFAALGWKLDFSHKLLFVPVEIFHNQDGYRGLNSEGAVVLRTRTGVRVPLSYGFNATA
                     QINLDWESDPAPDREELDRTVLLGLGYEFK"
     gene            6472..7563
                     /locus_tag="VKZ48_01810"
     CDS             6472..7563
                     /locus_tag="VKZ48_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HLU76622.1"
                     /translation="MKPNESAAAASGADDATLAQRLRADLGRLSPAYFGMVMATGIVS
                     LAAHLLGHGTVAWLLFQLNLVAYVVLWVLTLARAALHPRRFGADMVDHLRGPGFFTCV
                     AGSCVLACQFILLDSDYGSALVLWGLSVVLWVGLTYTVFTAFTIKENKPTLDQGISGG
                     WLLAVVATQAIAVLSALLAMREAQPYRLELNFFALSMWLWGGMLYIWMMSLIFYRYAF
                     FRFSPGDLAPPYWINMGAMAISTLAGSLLIINAPDAPFLHSLLPFLKGFTVFYWATGT
                     WWIPMLLVLGVWRHIYKRYPLRYDPLYWGAIFPLGMYAAATHEMTLAMNFHFLDALPL
                     AFLYAALLAWSLAFIGVVADVGKRLLGRH"
     gene            7614..8459
                     /locus_tag="VKZ48_01815"
     CDS             7614..8459
                     /locus_tag="VKZ48_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLU76623.1"
                     /translation="MKQHRFGTTGREVPVIGQGTWYIETGSRKNAVAALRKGLDLGMT
                     HIDTAEMYGGGASEELVGEAIAGRRDEVFLVSKVLPSNASRRGTIAACERTLKYLGTD
                     RLDCYLLHWRGSYPLIETFEAFEDLKAAGKILSWGVSNFDESDMQEALDVAGPGMIAC
                     NQVLYYPQERAIEHAVIPWCERNGVTVVAYSPFGHGEFPDEDSKGGRVLREIADAHGA
                     TPRQIALAFLVRNPTVVTIPKSGSPAHTEENAGGGSIALSDAEIARIDAAFPRGPKPR
                     HLPML"
     gene            8539..8922
                     /locus_tag="VKZ48_01820"
     CDS             8539..8922
                     /locus_tag="VKZ48_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76624.1"
                     /translation="MAWTVRYLSQGADIRFSGRVGVQEICDAQREAIRHAYEGPMRFN
                     VFDYADADDVHLETGDLIRIFDGRKDHLREHPPYALVLIGDQPILRDLDRLWDVLVRD
                     IPLETVLLPNREEAQRWLRDNGYSG"
     gene            complement(8910..9185)
                     /locus_tag="VKZ48_01825"
     CDS             complement(8910..9185)
                     /locus_tag="VKZ48_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006164639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76625.1"
                     /translation="MATGRLRGKARPFWERGYKSHGYWAGRRKLGAVRLHDDAPPEEK
                     YRWEAGTHAGHTASLIEAKRAVEAAVLLGQSQLPLFQDEAETADQPE"
     gene            complement(9312..12014)
                     /locus_tag="VKZ48_01830"
     CDS             complement(9312..12014)
                     /locus_tag="VKZ48_01830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLU76626.1"
                     /translation="MSARPNRAVSLTAILITVVIAVLNAGWWMLNNQPSAHPDWTGKV
                     NGLAYSGFQRHQDPTQKRFPTQEELAQDMALMAKVTKRIRTYTSTENPTVPALAEKAG
                     LKVTAGAWLDTRAENNENEIQALVQAVREHGNVDRAIVGNEAILRGDVSVRDMTRYIK
                     QVRGKINVPVSTAEPWHIWMRYPELARSVDYITIHLLPYWEGVPVDQAVNYVLDRYWE
                     VKRAYPRKEVVIGEVGWPSQGDRRDGAVASVENQAAFVRAFLERTIDLDLDYYFMEAF
                     DQPWKQAHEGRAGAYWGVFHADRTAKYPLAGPVISDPVWSAKALAASLLAAPLMFFFA
                     SRFRRFRFAGKFFYCALIQASVTLMVWLVGVPFEFYLDALDWAMFLLLLPALTAMLAF
                     LLTNAFEFVEVKWRGKWQREFHPIESPGAPQPFVSVHLPCHNEPPAMVILTLESLKRL
                     DYENYEVLVLDNNTKDPAVWRPVQEWCEQAGERFRFFHLDDWPGFNAGALNFGLTQTD
                     PRAEVIGVVDADYVVEPEWLKVLTPHFSNPKVAVVQAPQAHRDYEHSTFQRMCNWEFD
                     GFFRVGMHHRNERNAIIQHGTMTLVRKHALESTGSWSEWCICEDAELGLRLMNAGYEL
                     NYVDAVLGRGLTPSHFKAFKSQRFRWAFGAMQILKRRWNWLTKPGPLDAGQRFHFLTG
                     WFSWFADALHLVFTIAALLWTIGMITLPQYFSMPLDLFLLPVLGFFFCKAFFGPALYR
                     ERVNCSWLDVIGASVASMGLSHAIARGVFAGLTQKKGTFVVTAKGINGGGWLGAFAPV
                     REEGLMGVALVIGATLTLGTMGFDHREAKLWAMMLGAQSIPYWSAVICAAISARDAIR
                     ARQQAATPAKVVTRPAPAPANVIPLPAPVAAQAHAHGNGRVSTG"
     gene            12292..12684
                     /locus_tag="VKZ48_01835"
     CDS             12292..12684
                     /locus_tag="VKZ48_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76627.1"
                     /translation="MKRLQVFGTIGGVLVLALLARHLWIEPMAIGDACRVADTPLWCD
                     LRAMLILSFRYNGLGYAAVVLGVLSLFMHRMGIALAGASLGVAGLVLYCYDFAAVGLA
                     LSMLALARMTSPDGDVGGHQHGEREQQA"
     gene            complement(12598..14265)
                     /locus_tag="VKZ48_01840"
     CDS             complement(12598..14265)
                     /locus_tag="VKZ48_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76628.1"
                     /translation="MRAVIVAVLAIAAASALGYWWRLGQPVEVADAVKERFECVSYAP
                     FRRPGHSPLIKGTVVSPSQIEADLAILSRHTDCVRIYSVDQGLSETPRIARKFGMKVL
                     LGLWIGPNLEDNEREIELGLQIIQRDADSIRAVIVGNEVLLRKDQSVPALRSYIERIR
                     LQTDLPVSYADVWEFWLRYPEVAQSTSFITIHILPYWEDDPTPIELAIDHVVDIYEQM
                     HEAFPDQPILIGEAGWPSVGRNRYGAQPSLVNQTRFVRELINAAEAHELHYNLIEAFD
                     QPWKRRLEGTVGGYWGLYTAEGRLKFPFAGPVIEQPQWQHAILAGLAGAILLVLSAWM
                     NGPRPGGLTVIALLLGGFAAGATLYSQWQALEHSSRNSLEWFVTGFYSLCAVWAALKL
                     GQALNRWFEIGATAKPAPAAAIVRWFATNDDTYRPLERTLGMLRTLFLFGMSVVCLLQ
                     VFDPRYRDFPLALYAIPVAGFALLSFVSLTPPYALTRDRRPHARVEERWMAWWLLPAA
                     AWIVIHEGPYNPHAMLWGVLCLLFSFAVLMPAYVAVRRRHASERQHA"
     gene            complement(14346..14978)
                     /gene="pdxH"
                     /locus_tag="VKZ48_01845"
     CDS             complement(14346..14978)
                     /gene="pdxH"
                     /locus_tag="VKZ48_01845"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015876850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HLU76629.1"
                     /translation="MNLSELRKEYTREALDETHVASDPLDQFSHWLAAAIQAKLPEPT
                     AMTLATADAQGRPSARTVLLKSVDAQGFSFYTNYDSRKGQELAANPHASLLFFWPELE
                     RQVRVEGRVERVSTQESDEYFHSRPLGSRIGACASPQSRVVESREWLDQRMRQLSIEL
                     GEHPARPTHWGGYRLTPESIEFWQGRPSRLHDRLRFVRTDKGWRLERLAP"
     gene            15069..16019
                     /locus_tag="VKZ48_01850"
     CDS             15069..16019
                     /locus_tag="VKZ48_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLU76630.1"
                     /translation="MSVIDYDYGISAVDSGYVRPQLDAIHLIVERGRAAVVDTGTQHS
                     VAPVLAALRDKGIAPEAVDYVILTHIHLDHAGGAGALMQALPAARLVVHPRGVRHMVD
                     PSRLVEGTVAVYGAAETQRRYGDILPVPAARVLAAPADGMQLELAGRALSFYETPGHA
                     RHHVVVHDARSGRVFAGDTFGLSYRELDEGDRQFLFPTTSPVQFEPEPYHRSVDLIAS
                     LAKDAVYLTHYSELRRPRERVQDLHRLIDAHAAIALRHAGTTGAERERRLYEGVCELL
                     LTEARRYGTSLSDEEVMAVYGEDAALNAQGLAVWLDARVR"
BASE COUNT         2733 a         5265 c         5376 g         2975 t
ORIGIN      
        1 cggtcgcgca tcgactcgga aatcttgcgc gccatctcgc gcgcttcctc ggtgtcgtag
       61 cgcaggcgca gcatgatcag cgtgtcgccg agtccggtga agcccagacc cacgcggcgc
      121 ttcgccttcg cttcctcatg ctgctgttcg agcggccagt gggtcgcatc cagcacgttg
      181 tccagcatgc gcgtggccac atccacgacc ttgccgaagg cggcgaagtc gaatgccgcc
      241 ttgtcggtga aggggttcgt cacgaacttg gtgaggttga tcgagcccag gcagcagcag
      301 ccgtagggcg gcagcggctg ttcggcgcag gggttggtcg cctcgatcgt ctcgcagtaa
      361 taaaggttgt tgtccttgtt catgcggtcg atgaacagca cgcccggttc ggcgtggttg
      421 taggtcgagc gcatgatctg gtcccacagg tcgcgcgcgc gcaccttgcg gtacacccac
      481 aggccgtctt cgcgctgata ggcgcccgca tccttgacgt cctgcgcggg ctcggccttg
      541 tgcaccagtt cgacgtcgtc gtccttctct accgcttcca tgaagggatc ggtgacgccg
      601 acggagatgt tgaagttggt cagatcgcct tcgtccttgg cgtggatgaa ggcctcgatg
      661 tccgggtgat cgcagcgcag cacgcccatt tgcgcgccgc ggcgcgcgcc ggccgattcc
      721 accgtctcgc acgagcggtc gaacacgcgc atgtacgaca cggggccgct cgcacgcgat
      781 tgcgtgccgc gcaccagcgc gcccaccgga cggatgctgg agaagtcata gcccacaccg
      841 ccgccgcgac gcatggtctc ggccgcttcg gccagcgcgg tgtagatacc ggggcggccg
      901 tccaccactt cggtgacgga gtcgcccacc ggctgcacga agcagttgat cagggtggcg
      961 gacagcgtgg tgccggcggc ggaattgatg cggccggcgg gcacgaagcc catttcctgc
     1021 gcttccagga acttctgttc ccagtaggcg cgcttgtcct ccggttcgac ggcggccaag
     1081 gcacgggcga cgcggcgacg cacttcctcc agcgtgcgtt cgccgccctt ggcgtatttc
     1141 tcgatcagca cttccgcgca gatttcctgt tcggcgggcg ccgtgacggg ggcgactttg
     1201 gttttttggg cgctcatggg caagaacctc ttataactgt ttgaaggcag cggagcccgt
     1261 ctggcgtctc cgcaaccgtg attccggtcg ggcgtcgatc tcagggttgt aactactata
     1321 tgtagtagtt attcacccat taagaaactg attctaggtg gccgcttcgc gggaagcaac
     1381 tgccggtcac aataatttta gaaagactct aatcagtgcg aggctcaggg cgtgagccgc
     1441 gtcaaaatcg cgcaacgcac atttctagca cgggtgagct agccttggca ctcttgacaa
     1501 acggcaatcg cccggcggcc cgcatgcgga tgcgcgaagc gcttgttgca tcgatttttt
     1561 tattttttgt atcggtcccg gctgcagcgg tggagccgcc tatcgagctc accatcgccg
     1621 ccttcaatga cctccacgga cacctcgagg cgccgcgcgg cgaagtcggt ggcgtcgccg
     1681 caatggcggc ccggatcgac gcgctgcggc gcgagcggcc gcacacggtg gtggtgtccg
     1741 cgggcgatct gatcggcgcc agcccgctcc tgtccggact gttccgcgac gaaccgacca
     1801 tcgaggcgat gaaccgcctc gggctggatt tcgccgccgt gggcaatcac gaattcgacg
     1861 ccggtgcggc ggagctgcgt cgcctgcaga cgggcggctg ccatgccagc ggcgtgcgca
     1921 cgtgcaacgg ggcgcaggcg ggaacgtccg tacccttcga aggcgcgcgc ttccggtttc
     1981 tggcggccaa tgtggtcgat cgccgcaccg gcgagacgct gtttccgcct tatgccgtac
     2041 gccgcttcga cggcgtgccg gtggctttca tcggccttac cctgcgcggg acggcgggca
     2101 tcgttcgcgc ggccggcatc gccgggctgc gtttcgagga cgaggcgcgc acggtgaacc
     2161 gcctagtgcg caggttgcgc gccgagggcg tcgaggcgat cgtcgttctg ctgcacgaag
     2221 gcggtcggca cagcgggcat cccgacgaat gcgtcgattt cgacgggccg atcgtcgata
     2281 tcgtggcgcg gctcgacgac gcggtcgatc tggtggtgtc cgggcacacg catcaggcct
     2341 atcactgccg tctgcccaat gccgccggtc gcgccattcc ggtcaccggc gcgggcagct
     2401 acgggcgtta tctgacgcgc atcgacctgc gcctggatgc ggccagcggc gatgtgcgcg
     2461 gcgtcgaggt gcacaacggc gcggtgcggc gcgacggacc gcagcatccg gcggtggcgc
     2521 aactcgtgcg cgactatgcc gcactggccg agccgtttgc gcagcgggtg gtcggtcgca
     2581 tcgctgcgga cttcacccgc gacccggatg cggcgggtca gtccaccctg gggcaactca
     2641 cggcggacgc ccaacttgcc gtgacgcgcg acgccggggc ggtggcggcc tttatgaaca
     2701 tgggcggcct gcgtgccgat ttacgtgcgc gttcgtcggg cgaggtcact tacgaagacc
     2761 tctatgccgt tcaccccttc ggcaatgcgc tggtcacgct gacgctgagc ggcgcgcaga
     2821 tcaagaccat gctggagcag cagttcggct gtccgcgcga gcgctcacgg ccgcgccctt
     2881 tgcaggtgtc gagcggtttc gagtaccgct ggaaccctgc cgccgcgccg tgcgcgcgcg
     2941 tcgtcgccga gtcgatacgc gtgcagggcg ccccattggc gccgacgcaa cactatcgca
     3001 tcaccgtcaa cgatttcctg gccgacggcg gcgacggcct gcgtgtgctg cgcgagggca
     3061 cggagcgcac agggggtatg ctggatcgcg acgccctcgc atcctatttg caacaacacg
     3121 ggcccgtgcg ccctgcgccc acgcagcgcg tgcggctcgt gcactgataa aggaacgcag
     3181 catgtcgaag cgcatttctc ccgatgacat tccgaccggc aaggaggtgg ccacgctggg
     3241 tggcggttgc ttctggtgtc tggaggccgt ttacgacgaa ctgaagggcg tcgattccgt
     3301 cgaatcgggc tatatgggcg gcgaggtgga caacccgacc tacgagcagg tgtgcgacgg
     3361 cagcacggga cacgccgaag tggtgcgcat cactttcgat ccgcacgtga cgacctatca
     3421 ggacatcctg caggtgttct tcgtcatcca cgatccgacc acgctgaacc gccagggcaa
     3481 cgatgtcggg acgcaatacc gctcggcgat cttctatcac accgagacgc agaaggaggt
     3541 tgccgagctc gcgatcgcgc gactgggcga ggcgcaggta tggccgcggc cgatcgtcac
     3601 cgaagtggtg ccggccggcg acttctggat ggcggaggac tatcaccagg agtactaccg
     3661 gcgtaacccc aaccagtcct actgcaattt cgtggtcgag ccgaaagtgc agaagttcag
     3721 gaaggcgttc atggagaagc tgaagaagtg acgtgccttc ttccgcattc ctgcgcggac
     3781 gggaatcggg gtcgcaggcg gatgactccg cttcaagtct gtatcgacag cgcctgccac
     3841 aagctgtcca gttccgacgc gagtcgctcg tacacgcatc cgtgcagcac cagcagatcg
     3901 cggtcgctca ggttctcgaa cgacaggccg tagaagctcg ccagcggatg ctgggcgtcg
     3961 ctcgcgccgt acgtggaggc gatgcaggcg tcgagcgcca gcttgtattc gcgttcgccc
     4021 acgcgcagct tcagttcgat ctgcgccttg tcgcccttgg cgaggatgag atggttcggc
     4081 gtcgccacgc gtgcgccctg tgcggacagg tccacgacga ggcagcgctc cttgcgcgtc
     4141 gcggttttga tttcgctgac cagcgccatc gcggcgcgcg ggcttttgcg caccgaacgc
     4201 tgatcgacca gtcggggcat atcgatgtgc agatgcggaa agggctcgaa gctcactttc
     4261 agcactgtgg cggtgaagcg gaacaccgac gtcgcgtgaa aagtccggaa cacccattgt
     4321 tcgccgtcga gcaccggcac caggctcttg tccggccgga cggggtgact gaggatcagg
     4381 ctcttgcgct gatgcatgcc gatcacccag gtggtatagc cttcctgcga gtcgctgggc
     4441 gccatgtgca agccggacat cgaacggcgc gcggtttgtg cataggcgcg gctcaaccgc
     4501 aggatggggc agttgcgctc ttcctcttcc ttgagggcgt tggcggcatc ctgcggattg
     4561 ccggtccggc tgcacgacac acccgtcgta cgcagcagtt cgcacatgcg ttcgctttcg
     4621 acgatctggc cttgcgcgag caacagcctg ccgtcggccg catagacgga gaacggcagg
     4681 accttgccgg tctcaatggc gtccgggtcc agcaaagctt cacgagtgct cacaggcgtt
     4741 cctcatcgct gcggttatgg aaagcgatgc cccgggcatt cctcccctta cgctgtatcg
     4801 gcctggccgg cgtgaatctt tatggcgcgc agtgcagtcg gcggcgaggc gtgtggattt
     4861 gtgccgcttc ccgatgtccg gaggtgcggg cggtgccggc ccgatttttg cgcgccgggc
     4921 cgtactgccg acgagtgtga agcgcttcag catcgcgctt cattcggtcc gcttcggacc
     4981 ttgcgccgtg cgggcccggc ccgtacccta acggcgctgt cgcggtacac ctcagtggat
     5041 ttcacatcgt ttatcccatc cgagcatgcc tgcgggcgtg cttgcgcagt gccacagcac
     5101 ccaaccatcg aggaggctcc gattcggctt gtctgatcga tgtcgtcgcg caaggtcgcc
     5161 gtcttgcctg cacggccccg cgcgttcgat cgtcgtcgcg tctgatccgg cttcctccgt
     5221 ccggccgcgt gcagcgcttg cgcggccggt gctttgttca tcgggttccc cggccctcgg
     5281 gccgtgagcg gaaccgttca tattcgggag acagggatgt tcatcaacgt cctgcgatcc
     5341 gtatttgcgg gcgtgacgct gctctgcgcg gtgcaggccg cgcacgccga ccgcctggtc
     5401 ctgaaaaacg gcgacgtgct gaccggcacg gtgttgcaca agagcggcga cagcctgagc
     5461 ttcagcaccc cttatgccgg cgtgatcagt gtgagctggg cggcggtccg cacgctcacc
     5521 accgacaagg ccgtcaacgt gatgctgaac gacgccacgg actatgacgg acgtctcgac
     5581 gcgatgggcg acggccgcgc ggtgttgacg ctcagcaagg atccggtcac gggcgcagcg
     5641 gacgaggagc acgaaggcga acccacgctc gcgcaggaag aggccgcgcc gcgggccgag
     5701 ctcgatctgg ccaaggtgct gtacatcaac cccacgccgg aggagtcggg cgagggctat
     5761 cgcatcaccg gccgggcgaa cgtggcctac tcgaacgcca gcggcaacac cgagaacgaa
     5821 cagctgcacg gcaacgcgga aatggtgctg cgcgcgaaga tgtaccgcta cgtgctcggc
     5881 ggcgaggcaa cgcgcgcgag cgacaacggc aacgtctcgg cccacaacgc gcgcctttac
     5941 accaactacg actggttctt caggccgaag cagttcgcct acgccgccgc atcggtcgag
     6001 cgcgaccggt tcaaggacat ccatctgcgc agcgtcgtcg gcggcggtta cggttatcag
     6061 tggatcgaaa ccgacgtcac caagctgtcg ctgcgcggcg gtctcgacta cgtcaccatc
     6121 gatcactacg acgcggagaa cgaacacttc gccgcgctgg gctggaaact ggacttcagc
     6181 cacaagctgc tgttcgtgcc ggtcgagatc ttccataacc aggacggcta ccgcggcctc
     6241 aacagcgagg gcgccgtcgt gctgcgcacg cgcaccggag tgcgggtgcc gctgagctac
     6301 ggcttcaacg ccacggcgca gatcaatctc gactgggaaa gcgacccggc gccggatcgg
     6361 gaggagctcg atcgcacggt tctgctcgga ctcggctacg agttcaagta agctgcgtca
     6421 ctttccgtca cgcggttgcc gcatcatgag tctgcgcatc gcacatccgg catgaagccc
     6481 aacgagtcgg cggcggccgc ttcgggcgcg gacgacgcca cgctggcgca gcggctgcgc
     6541 gccgatctcg gccggctctc gcccgcctat ttcggcatgg tgatggccac cggcatcgtt
     6601 tcgctcgccg cgcatctgct cggtcacggc accgtcgcgt ggctgctgtt ccagctcaac
     6661 ctggtcgcct acgtcgtgct gtgggtgctg acgctggcgc gcgcggcatt gcacccgcgt
     6721 cgcttcggcg cggacatggt cgaccacctg cgcggcccgg gcttcttcac ctgcgtggcc
     6781 ggctcgtgcg tgctcgcgtg tcagttcatc ctgctcgatt ccgactacgg cagcgcgctg
     6841 gtgctgtggg gtctttcggt cgtgttgtgg gtggggctga cctacaccgt cttcaccgcc
     6901 ttcaccatca aggagaacaa gcccacgctc gatcagggca tcagcggcgg ttggctgctc
     6961 gccgtcgtcg ccacccaggc catcgccgtg ctgagcgcgc tcctggccat gcgcgaggcg
     7021 cagccctacc gcctcgaact caacttcttc gcgctgtcga tgtggctgtg gggcggcatg
     7081 ctctacatct ggatgatgtc gctcatcttc tatcgttacg ccttcttccg tttctcgccg
     7141 ggcgacctcg cgccgcctta ctggatcaac atgggcgcga tggcgatttc cacactcgcc
     7201 ggttcgctgc tcatcatcaa cgcgccggac gcgcccttcc tccattcgct gctgcccttc
     7261 ctcaagggtt tcacggtgtt ctactgggcg accggcacct ggtggattcc catgctgctg
     7321 gtgctgggcg tctggcgcca catctacaag cgctatccgc tgcgctacga cccgctgtac
     7381 tggggtgcga tctttccgct cggcatgtac gcggccgcca cgcatgagat gacgctggcg
     7441 atgaactttc attttctgga tgcgctgccg ctcgcctttc tttacgcggc gctcctggcg
     7501 tggtcgcttg cgttcatcgg cgtcgtggcg gatgtgggga agcggctgct cggccggcac
     7561 tgagcggaat cgaaaggcga taataggcgg cgacaaattt tctgaggacc atcatgaaac
     7621 agcatcgatt cggcacaacg ggccgcgaag tgcccgtcat cggccagggc acctggtaca
     7681 tcgaaacggg cagccgcaag aacgccgtcg cggcgttacg caaaggcctc gatctgggca
     7741 tgacgcacat cgacaccgcg gagatgtacg gcggcggcgc gtcggaagaa ctcgtcggcg
     7801 aagccatcgc cggccgtcgc gacgaagtct ttctcgtctc caaggtgctg ccttccaacg
     7861 cgtcgcgacg gggcaccatc gccgcgtgcg agcgcacgct gaaatatctg ggtactgacc
     7921 ggctggactg ctatctcctg cactggcgcg gcagctatcc gctgatcgaa accttcgaag
     7981 ccttcgagga cctcaaggcc gcgggcaaaa tcctctcgtg gggcgtgagc aacttcgacg
     8041 aaagcgacat gcaggaagcg ctcgacgtgg cgggtcccgg catgatcgcc tgcaaccagg
     8101 tgctctacta cccgcaggag cgcgccatcg aacacgcggt gattccctgg tgcgaacgca
     8161 acggcgtgac ggtcgtggcc tacagcccct tcggtcacgg cgagtttccg gacgaggatt
     8221 ccaagggcgg ccgtgtgctg cgcgaaatcg ccgacgcgca cggcgccaca ccgcgccaga
     8281 tcgcgctggc cttcctggtg cgtaatccga ccgtggtcac catcccgaag tcgggcagcc
     8341 ccgcacacac cgaagaaaac gccggaggcg gcagcatcgc gttgagcgat gcggagatcg
     8401 cgcgcatcga tgccgcgttc ccgcgcgggc cgaagccgcg gcatctgccc atgctctgag
     8461 cagcgggcat gacgctcgca cgcctcccgg tgccgggcgg ctgtcgccgc ccgaccgcta
     8521 tatcgcgccg ggggtgtaat ggcttggacc gttcgttact tgtcgcaggg cgcggacatc
     8581 cgcttcagcg ggcgtgtcgg cgtgcaggag atttgcgatg cgcagcgcga ggcgatccgg
     8641 cacgcctatg agggtcccat gcgcttcaac gtcttcgact acgccgatgc cgacgacgtc
     8701 catctggaga cgggcgacct gatccgcatt ttcgacggtc ggaaggacca tctgcgcgag
     8761 catccgccct atgcgctcgt actcatcggc gaccagccga tcctgcgcga cctggaccgg
     8821 ctttgggatg tgctggtccg cgacattccc ctggagacgg tgctgctgcc gaaccgtgag
     8881 gaggcgcagc ggtggctgcg cgacaacggc tactccggtt gatcggccgt ttccgcctcg
     8941 tcctggaata gcggcaactg cgattgcccc agcagcacgg ccgcttcgac ggcgcgcttg
     9001 gcttcgatga gcgatgcggt gtggcccgca tgcgtgccgg cttcccagcg atatttttct
     9061 tcgggcggcg cgtcgtcgtg cagacggacg gcgcccagct tgcgccggcc cgcccagtag
     9121 ccgtgcgatt tgtagccgcg ttcccagaac gggcgcgcct tgcctctcaa tctgccagtc
     9181 gccatgtgcc gctgctcccg atgtccgggg cgaatgctaa ggaagggcgg cgcgactgtc
     9241 caatcgcgcg cccggcgcgc aaatgaaaac cgcggctcgg agccgcggtt ttcgtgtgat
     9301 gccgcacctt gttaaccggt gcttacccga ccattgccgt gagcgtgcgc ctgcgcagcc
     9361 accggtgcgg gcaggggaat gacgttggcg ggtgcaggcg ccggccgcgt gaccaccttc
     9421 gccggcgtgg ccgcctgctg acgtgcgcgg atggcgtcgc gtgcggaaat cgcagcgcag
     9481 atcaccgctg accaatacgg aatcgattgc gcgccgagca tcatcgccca cagtttggct
     9541 tcgcgatgat cgaagcccat ggtgccgagg gtgagcgttg cgccgatcac cagcgcgacg
     9601 cccatcaggc cttcctcgcg gacgggcgcg aaggcgccca gccagccgcc gccgttgatg
     9661 cccttggcgg tgacgacgaa ggtacccttc ttctgcgtaa ggccggcgaa cacgccgcgt
     9721 gcgatggcgt gcgacagacc catgctcgcc accgatgcgc cgatgacgtc cagccagctg
     9781 cagttcaccc gctcgcgata aagcgccgga ccgaagaacg ccttgcagaa gaagaaaccc
     9841 agcaccggca gcaggaacag atccagcggc atgctgaagt actgcggcag cgtgatcatg
     9901 ccgatggtcc acaacagcgc cgcgatggtg aacaccagat gcagcgcatc ggcgaaccag
     9961 gagaaccagc cggtgaggaa gtggaagcgt tgtcccgcat ccagcggtcc cggcttggtc
    10021 agccagttcc agcggcgctt gagaatctgc atcgcgccga aggcccagcg gaagcgctgc
    10081 gacttgaagg ccttgaagtg cgatggcgta aggccgcggc cgagtacggc gtcgacgtaa
    10141 ttcagctcgt agcccgcgtt catcaggcgc agacccagtt ccgcgtcttc gcagatgcac
    10201 cattccgacc agctgccggt cgactccagc gcatgcttgc gcaccagtgt catggtgccg
    10261 tgctggatga tggcgttgcg ctcgttgcgg tgatgcatgc cgacgcggaa gaagccgtcg
    10321 aactcccaat tgcacatgcg ctggaaggtg ctgtgctcgt agtcgcgatg tgcctgcggc
    10381 gcttgcacga ccgccacctt gggattgctg aagtgcggcg tcagcacttt caaccactcg
    10441 ggttcgacca cgtagtcggc atccaccacg ccgatgactt cggcgcgcgg atcggtctgc
    10501 gtcaggccga agttgagcgc gccggcattg aagcccggcc agtcgtccag gtggaagaag
    10561 cggaagcgct cgcctgcctg ttcgcaccat tcctgcaccg gacgccatac cgccgggtcc
    10621 ttggtgttgt tgtccagcac cagcacttcg taattctcat agtcgaggcg cttcaggctt
    10681 tccagcgtga ggatgaccat cgccggcggt tcgttgtggc agggcaggtg caccgagacg
    10741 aagggctgcg gcgcaccggg gctttcgatg gggtggaatt cgcgctgcca tttgccacgc
    10801 catttcacct ccacgaactc gaaggcattg gtcagcagga acgccagcat cgcggtcagc
    10861 gccggcagca gcagcaggaa catcgcccag tcgagcgcgt cgagatagaa ctcgaagggc
    10921 acaccgacca gccacaccat cagcgtcacc gacgcctgga tcagcgcgca gtagaagaac
    10981 ttgccggcga agcggaagcg gcggaagcgc gacgcgaaga agaacatcag cggcgctgca
    11041 agcagcgatg ccgcgagcgc cttggccgac cacacggggt cggagatcac cggtccggca
    11101 agcggatact tggccgtgcg gtccgcatgg aacacgcccc agtaggcgcc ggcgcgacct
    11161 tcgtgcgcct gcttccacgg ctggtcgaag gcttccatga agtagtagtc gaggtccagg
    11221 tcgatggtcc gctcgaggaa ggcgcgcacg aacgccgcct ggttctccac cgaggcgacg
    11281 gcgccgtcgc gacggtcgcc ctggctgggc cagcccactt cgccgatcac cacttccttg
    11341 cgcggatagg cgcgcttcac ttcccagtag cggtcgagca catagttcac cgcctgatcg
    11401 acgggcacgc cttcccagta gggcagcagg tggatggtga tgtaatcgac gctgcgcgcg
    11461 agttccgggt agcgcatcca gatatgccac ggctcggcgg tggacaccgg cacattgatc
    11521 ttgccgcgca cctgcttgat gtagcgggtc atgtcgcgca cgctcacgtc gccgcgcagg
    11581 atcgcttcgt tgccgacgat ggcgcgatcg acgttgccgt gttcgcgcac ggcctgcacc
    11641 agcgcctgta tttcgttctc gttgttctcg gcgcgcgtgt ccagccacgc gcctgccgtc
    11701 accttcagtc cggccttttc cgcaagtgcg ggtaccgtcg ggttttcggt cgaggtatag
    11761 gtccggatgc gcttggtgac cttggccatc aacgccatgt cttgcgccag ttcctcctga
    11821 gtggggaagc gcttctgggt cggatcctga tgacgttgaa agccgctgta ggccagtccg
    11881 ttcactttgc cggtccagtc cggatgcgcg ctcggctggt tgttcaacat ccaccagccg
    11941 gcgttgagca cggcgatgac aacggtgatc agaatcgccg tcagcgagac ggcgcgattt
    12001 gggcgtgcgc tcaaggtaaa agacctcgtc aattaattgc gctgcagtag gtaggcatcg
    12061 cgctatgcgc gatgcaatcc ggaacggaag cagcggtttc gttcttgcag gaagcttgcc
    12121 tgcccagacc gattgattag tgcggtggca tatccacgcg tcacgcgtac agggatgtat
    12181 cgttcaaact caaacgcaac gtattctatc gatgcgtcta aggacaaaac agcttcgatc
    12241 gactaaaact atgtcgggta tcgacgacag aacgatgagg gcggcgacgg catgaagagg
    12301 ttgcaggtat tcggaaccat cggcggagtg ctggtgctgg cgctgcttgc gcggcatctg
    12361 tggatcgaac cgatggcaat cggcgatgct tgtcgggtcg ccgacacgcc gctgtggtgt
    12421 gacctgcgcg caatgctgat cctgagcttc cgctacaacg ggctgggcta cgccgcagtc
    12481 gtgctgggcg tgctgtcgct gttcatgcat cggatgggaa ttgcgctggc gggtgcgagc
    12541 ctgggcgtcg cggggctcgt attgtactgc tacgacttcg ccgcggtggg tctggcctta
    12601 agcatgctgg cgctcgctcg catgacgtcg ccggacggcg acgtaggcgg gcatcaacac
    12661 ggcgaacgag aacagcaggc ataagacgcc ccacagcatg gcgtgcgggt tgtacggtcc
    12721 ctcgtggatc acgatccacg ccgcggccgg cagcagccac cacgccatcc agcgttcctc
    12781 cacgcgcgca tgcggccgcc ggtcgcgcgt gagggcgtag ggcggcgtaa gcgacacgaa
    12841 cgacagcaag gcgaagcccg ccaccggaat ggcatagagt gcaagcggga agtcgcggta
    12901 gcgcggatcg aatacctgca acaggcacac gaccgacatg ccgaacagga acagcgtccg
    12961 tagcatgccc agcgtgcgtt ccagcggccg gtaggtatcg tcgttggtgg cgaaccagcg
    13021 cacgatggcg gcggccggcg ccggcttggc cgtcgcgccg atttcaaacc agcggttgag
    13081 cgcctggccg agcttcagtg ccgcccacac ggcacagagc gaatagaaac ccgttacgaa
    13141 ccattccagt gaattgcggc tggaatgttc gagtgcctgc cactgcgagt acagcgttgc
    13201 acccgccgcg aagccgccca gcagcagcgc aatgacggtg agtccgcccg ggcgcggccc
    13261 gttcatccac gccgacaaca ccagcaggat ggcgcccgcc agtccggcga ggatggcgtg
    13321 ctgccattgc ggctgttcga tcaccgggcc tgcgaacgga aacttcaacc gtccttcggc
    13381 ggtgtaaagc ccccagtagc cgccgacggt cccctcgaga cggcgcttcc acggctggtc
    13441 gaaagcttcg atcaggttgt agtgcagttc gtgcgcttcc gcggcgttga tcagctcgcg
    13501 gacgaagcgc gtctggttga ccaggctcgg ttgcgcaccg tagcggttgc gccccacgct
    13561 gggccagccc gcctcgccga tgaggatcgg ctgatcggga aaggcctcgt gcatctgctc
    13621 gtagatatcg acgacatggt cgatcgccag ttcgatgggc gtgggatcgt cttcccagta
    13681 cggcaggatg tgaatggtga tgaaggaggt cgattgcgcc acctccgggt agcgcagcca
    13741 gaactcccac acgtccgcat agctgaccgg caaatcggtc tgcagccgga tgcgttcgat
    13801 gtacgagcgc agcgcgggca ccgactggtc cttgcgcagc agcacttcgt tgcccacgat
    13861 cactgcgcgg atcgaatcgg catcgcgctg gatgatctgc agtccgagtt cgatctcccg
    13921 ctcgttgtcc tcgaggttcg ggccgatcca cagtccgagc aggaccttca tgccgaactt
    13981 gcgcgcgatc cgtggcgttt ccgacagtcc ctgatcgacc gaatagatgc gcacgcaatc
    14041 ggtatggcgg gaaaggatgg ccagatccgc ctcgatctgc gacggcgata cgacggtgcc
    14101 cttgatcagc ggggaatgcc cggggcggcg aaaaggcgcg taggacacgc attcgaagcg
    14161 ttccttcacc gcatccgcga cctcgaccgg ctgccccagt cgccaccagt accccagcgc
    14221 cgatgcggcc gcgatggcca gtacggcgac gatgactgcg cgcatttgtt ccactcccgt
    14281 ttacccgcgt agcggtgagc aacgaacgca cccgctggtg cttgcggtgc gtaaagggcg
    14341 ccgcttcagg gggcgagccg ctccagtcgc cagcccttgt ctgtgcgcac gaagcgcagc
    14401 cggtcgtgca gccggctcgg ccggccctgc cagaactcga tcgactcggg cgtcaggcgg
    14461 taaccgcccc agtgcgtcgg ccgcgcagga tgctcgccca gttcgatgct caactgccgc
    14521 atgcgctggt ccagccactc gcggctttcc accacgcggc tttgcggcga ggcgcaggcg
    14581 ccgatgcggc tgcccagcgg ccgactgtga aaatattcgt cggattcctg ggtcgagacg
    14641 cgctccacgc ggccttccac gcgcacttgc cgctccagct ccggccagaa gaacagcagg
    14701 ctggcgtgcg gattggccgc caactcctga cccttgcggc tgtcgtagtt ggtgtagaag
    14761 ctgaacccct gcgcatcgac gctcttgagg agcacggtgc gtgcggaagg gcgtccctgt
    14821 gcatcggcgg tggccagcgt catggcggtg ggttcgggca gcttggcctg tatcgcggcc
    14881 gcaagccagt gggaaaactg atccagcgga tcgctcgcga catgcgtttc gtcgagcgcc
    14941 tcccgcgtgt attccttgcg cagttccgag agattcatgt cgggtcgggc aggctccata
    15001 gagttgactt gcgcattcta cgccgcgccg gcctctacaa tgggactccg atcaaggggc
    15061 gtgcagtcat gtcagtcatc gattacgact acggcatcag tgcggtggat tcgggctacg
    15121 tccgcccgca actggacgcc atccatctca tcgtcgagcg gggccgcgcg gcggtggtcg
    15181 acaccggcac gcaacattcg gtggcgcccg tgctcgccgc gctgcgcgac aagggcatcg
    15241 cgcccgaggc ggtcgattac gtcatcctca cccacatcca cctcgaccac gccggcggcg
    15301 cgggtgcgct gatgcaggcg ttgccggcgg cgcgactggt ggtgcatccg cgcggtgtgc
    15361 ggcacatggt cgatccttcc cggctggtcg aaggcacggt cgcggtgtat ggcgccgccg
    15421 aaacgcaacg acgctatggc gacatcctcc ccgtgccggc agcgcgcgtc ctcgccgcgc
    15481 cggccgacgg gatgcaactc gaactggcgg gtcgcgcact cagcttctac gagacgcccg
    15541 gccatgcgcg tcaccacgtg gtggtgcacg acgcgcgctc gggcagggtg ttcgcgggcg
    15601 acaccttcgg cctgtcctat cgcgaactgg acgagggcga tcgccagttt ctgttcccca
    15661 ccaccagtcc cgtgcagttc gaacccgagc cctatcaccg ctcggtagac ctgatcgcga
    15721 gtctggcgaa ggatgccgtt tacctcacgc actacagcga attgcgccgc ccgcgggagc
    15781 gcgtgcagga cctgcatcgg ctgatcgacg cccatgccgc gatcgccttg cgtcacgccg
    15841 gtacgacggg tgcggagcgc gaacggcggc tgtacgaagg tgtgtgcgaa ctgctcctga
    15901 cggaggcgcg acgctacggc acctcgctgt cggacgaaga agtcatggcc gtgtacggtg
    15961 aagacgccgc cctcaacgca cagggtctcg ccgtctggct ggacgctcgc gtgcgctgaa
    16021 cggcagtcgg tttcctcctc gtttttccct ctgcacgggg cgttgcgcgg tgttgtcggc
    16081 tgtcggtcgc attgcgttgc ggcgcagcgt gaggcgcaaa gcatggacgc aaagcgctgc
    16141 ctttcttgtc gcgcgatggc gtcgcccgag tgaaaacact gttgcgacac tgcgacgtca
    16201 gagggaaaaa cgctcaaatt ccgcttgcgt gaatgcgtgt gcttgtgcat tctctcgtcg
    16261 cctagcccgc tgctctgggg cggtaacgca aaagatcgat gatcacaacg ctaagtggag
    16321 gtttaaggat ggcgacgaag aagcccgct
//
LOCUS       DATGOU010000033         8843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3184543, whole genome shotgun sequence.
ACCESSION   DATGOU010000033 DATGOU010000000
VERSION     DATGOU010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8843
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3184543"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2210
                     /gene="clpB"
                     /locus_tag="VKZ48_01855"
     CDS             <1..2210
                     /gene="clpB"
                     /locus_tag="VKZ48_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HLU76631.1"
                     /translation="LEAAITAVRGGEKVDSADAESQREALKKYTLDLTERARSGKLDP
                     VIGRDDEIRRAIQVLQRRSKNNPVLIGEPGVGKTAIVEGLAQRIVAGEVPDSLRDKRV
                     LVLDMAGLLAGAKYRGEFEERLKAVLKEVAQDEGRIILFIDEIHTMVGAGKAEGAIDA
                     GNMLKPALARGELHCVGATTLDEYRKYIEKDAALERRFQKVLVEEPDVEATIAILRGL
                     KERYEVHHGVDITDPAIVAAAELSHRYITDRFLPDKAIDLIDEAASRIKMEIDSKPEE
                     MDRLDRRLIQLKIEREAVRKETDEASQKRLALIEDEIERLEREYADLEEIWKAEKLAV
                     QGSQHLKEALERARQEMDEAKRKGDWQKMSELQYGRIPELEAQLKKADTGAAGAEKPK
                     LLRTEVGTEEIAEVVSRATGIPVSKMMQGERDKLLHMEEKLHERVVGQDEAVRLVSDA
                     IRRSRAGLSDPNKPYGSFLFLGPTGVGKTELCKALAAFLFDSEDHLIRIDMSEFMEKH
                     SVARLIGAPPGYVGYEEGGYLTEAVRRKPYSVILLDEVEKAHPDVFNVLLQVLDDGRM
                     TDGQGRTVDFKNTVIVMTSNLGSQMIQNMAGDEPSLVKLAVLGEVKANFRPEFVNRID
                     EIVVFHALDEKHIMNIARIQMRGLEERLAKLEMKLQITDEALEELAKVGFDPVYGARP
                     LKRAIQQEIENPLSKLILEGRFAAKDTIVVEYRDNQMRFRKAQPAEREAATGR"
     gene            complement(2255..2896)
                     /locus_tag="VKZ48_01860"
     CDS             complement(2255..2896)
                     /locus_tag="VKZ48_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HLU76632.1"
                     /translation="MNLLTKTVTALICMFIGALPVHAQSPGEVKDLHAVVLVATPALV
                     DAEYRQTVLLATITEGGGHVGIILNRPTQRSLGSLFPEHGPSKKVVEPVFFGGPFGIT
                     ALTAAVHTEQSPGPGSIIMMPGVFLAVGVQTIDKIIEERPNDARYYIGHVGWRPGELR
                     QEIRRGLWYVMPPDPEVVFRKDMDGLWEELVREARQVSAVLDPAFAPFTLNGL"
     gene            3049..3498
                     /locus_tag="VKZ48_01865"
     CDS             3049..3498
                     /locus_tag="VKZ48_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLU76633.1"
                     /translation="MYKKILMAYNGTQEGRTALLTCAELAAFVHAETHLLSVATMPSS
                     MFLTEGFVPEELIDEEKKRMQAVLDEGLELLAQRGFSVTGHLAVGEPVEEISRLAREL
                     DCDLIILGHKQQHSFAARWWRGSVGATLLDVSPCSILLTVTDGTAKN"
     gene            complement(3739..7455)
                     /locus_tag="VKZ48_01870"
     CDS             complement(3739..7455)
                     /locus_tag="VKZ48_01870"
                     /inference="COORDINATES: protein motif:HMM:NF017197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HLU76634.1"
                     /translation="MSTFSTIRQRLGAAIAHRRTRRAAVWLIGLVALYALLGFFVLPW
                     FAKPRIEQALGEALMRPVSIERLYTNPFSLSARIDNLQVRDADGGEAIAGIGEAYANV
                     SSTSLFRLAPVIDRLRIREPFIKLVRHEDRQYNVQDVVDRYVWQQPEAPEPEDKTPPR
                     FSLNNIELIDGRISFDDQLEGKRHEVRELQIGVPFLSSLPYATEIYVQPNLSAVVNGA
                     PLAVSGETKPFGEDRETLLYINFDNVQLPQYFDYVPVPLRFALAKGALDARLTLRMAG
                     APGSMTTLSLTGTATARDVDVRQRDDEPMIAFESLAVELRELDLIGNRLSLAAVRAAS
                     PQVHVTRFRGGMLNLELLMPLETGTAQKPAAASAESKPAQPFRYAVDEVAIANGRVQL
                     LDYDAPQPFRTAFTDIALNAKNIGNDPEQQGTYDVSLRLTDKGQFTTQGQLALAPVAV
                     NGNFKLADLALPHFQPYLGPERQLGSGTLSGEGAYRVAMKDGAAPDIVVEGLKATVRD
                     SRVHTAIGKDRWRAGQIDVSGVRIDVGKRNVSVDEVSTRDVAATVLRTANGDWYVTGL
                     LHVAAETAEAAEQAQKNPSAADDGPAWTYQVRRIGLEASSLQYEDRATKQPVKLKAHN
                     INGVLSGLSSAPDAEPQLALNATIQKAGKLSLGGPMKLAPFGFDWKVSAQAIPVLPFS
                     PYWTEQISLDITRGAIAARGRLRLGEDRRGQTALRFGGRIDVNDFSAREGGSRRDLLR
                     WKRLRLQDVYLRTEPVLVRVGAIALNDSFARIILLPDGTLNLQHVLAAPSDAKDEAAQ
                     QTVPGETVAANEVTASDDAATADDTAAPEETAAPKPAIERRGKPRIEIGEVRLANGEL
                     DFSDRYIKPNYRAEIGKLEGSIGALSPEQAGRIELTGTIDDIAPVTISGLINPLGQSL
                     FLDIDASAKGVNLPPLSPYSVRYIGYPIERGKLSMDVKYHIENDQLTAQNKLVLNQLV
                     FGEKVESPEALNVPVHLAVSLLKDSNGVIDLDVPISGSLNDPQFSIGGVIAKAIGNLF
                     IKVVTSPFTALGAMFGGDGERMSFIAFEPGSAELSEAAQQRLSRLARAMRNRPGLTLD
                     VAGRVDPQAEEDALRRESMMRRLRTAKLKALTEELDNPPRLEEVEIEPEEYEKYLKAA
                     YDAGRFDKPRNFLGFAKRLPPAEMEKLLLANTPVNENAMRRLANRRAEAVVTWLREQG
                     QVPPERVYVAAPKLDAKGVTGDGATTRVDFTVRR"
     gene            complement(7506..8429)
                     /locus_tag="VKZ48_01875"
     CDS             complement(7506..8429)
                     /locus_tag="VKZ48_01875"
                     /inference="COORDINATES: protein motif:HMM:NF039714.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HLU76635.1"
                     /translation="MHTLIARGALSLACAGFLLLVLGACSAPQLAYNSAPLVLRYKLY
                     NAFDPNDEQDVMMSAALRRLHDWHRHNELPAYAEMLDRLAVRVEAGLDAADIAQTREL
                     AQARYERLAAQAVDEAIPILRTLDARNFTALRRYVKERNEDYADEFLDGNEAERLEAM
                     VESLTDHVERWVGDLNATQTRLLTEFAARHPHFAELRLENRRHLQARTLAAVDALVRG
                     EAQAEAALREVIVHWERNSLPQYREALRAWEADFRATMLAIERSLTPAQRRTTVANLR
                     EYSGQLLALTENRSARPSPAAQAGLTRPPVR"
     gene            8509..>8843
                     /locus_tag="VKZ48_01880"
     CDS             8509..>8843
                     /locus_tag="VKZ48_01880"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HLU76636.1"
                     /translation="MASTGQRLKGHDGTASTALLLIDFINDLDFDGGEQLAPQAVAAA
                     QATAALRARADAASVPVIYVNDNFGRWQSRFDQAVAHCRRSGGRGRKLAELLTPREHD
                     YFILKPMHSG"
BASE COUNT         1515 a         2805 c         2972 g         1551 t
ORIGIN      
        1 cgctcgaagc ggcgatcacg gccgtgcgcg gtggcgagaa ggtcgacagc gccgacgccg
       61 agagccagcg cgaggcactg aagaaataca cgctggacct gaccgagcgc gccaggagcg
      121 gcaagctcga cccggtgatc ggccgcgacg acgaaatccg ccgcgccatc caggtgctgc
      181 agcgccgcag caagaacaac cccgtgctca tcggcgagcc cggcgttggc aagacggcca
      241 tcgtcgaagg cttggcgcaa cgcatcgtcg ccggcgaagt gcccgactcg ctgcgcgaca
      301 agcgcgtgct ggtgctcgat atggcgggcc tgttggccgg tgcgaaatac cgtggcgaat
      361 tcgaagagcg cctgaaagcc gtgctgaagg aagtggcgca ggacgaaggc cgcatcattc
      421 tcttcatcga cgagatacac accatggtcg gcgcgggcaa ggccgaaggc gccatcgacg
      481 cgggcaatat gctcaagccg gcgctggcgc gcggcgaact gcattgcgtg ggtgcgacca
      541 cgctcgacga ataccgcaag tacatcgaga aggatgcggc gctggaacgc cgcttccaga
      601 aggtgctggt ggaagagccg gacgtggagg cgaccattgc catcctgcgc ggcctgaagg
      661 agcgctacga agtccaccac ggcgtggaca tcaccgaccc ggcgatcgtg gcggcggccg
      721 aactgtcgca tcgctacatc accgaccgct tcctgcccga caaggccatc gacctcatcg
      781 acgaggccgc ttcgcgcatc aagatggaaa tcgattccaa accggaagag atggaccgtc
      841 tcgaccgccg tctcatccag ttgaagatcg agcgcgaggc ggtgcgcaag gaaaccgacg
      901 aagcctcgca gaagcgtctg gcgctaatcg aggacgagat cgagcggctc gaacgcgagt
      961 acgccgatct ggaggaaatc tggaaggccg agaagctcgc cgtgcagggc tcgcagcacc
     1021 tgaaggaagc gctcgagcgt gcccgtcagg aaatggacga agccaagcgc aagggcgact
     1081 ggcagaagat gagcgagttg cagtacggcc gcattcccga actcgaggcg cagttgaaga
     1141 aggccgacac cggcgccgcc ggcgcggaga agccgaaact gctgcgcacc gaagtcggca
     1201 cggaggaaat cgccgaagtc gtgtcgcgcg ccaccggcat tcccgtgtcg aaaatgatgc
     1261 agggcgagcg cgacaagctg ctgcacatgg aagagaaact gcacgagcgc gtggtgggac
     1321 aggacgaggc ggtgcgcctc gtgtccgatg ccatccgccg ttcacgtgcc ggcctgtccg
     1381 atccgaacaa gccctacggt tcgttcctgt tcctcggccc gacgggcgtg ggcaagaccg
     1441 agctgtgcaa ggccctggcc gcgttcctgt tcgattcgga ggatcacctc atccgcatcg
     1501 acatgtcgga attcatggag aagcactcgg tcgcgcgtct catcggcgcg ccgcccggct
     1561 atgtcggcta cgaggaaggc ggttatctca ccgaagcggt gcggcgcaag ccctactcgg
     1621 tgatcctgct cgacgaagtg gagaaggcgc atccggacgt attcaacgtg ctgctgcagg
     1681 tgctcgacga cggtcgcatg accgacgggc agggccgcac ggtggacttc aagaacacgg
     1741 tgatcgtgat gacctcgaac ctcggttcgc agatgatcca gaacatggcg ggcgacgagc
     1801 cgtcgctcgt caagctggcc gtgctcggtg aagtgaaggc gaatttccgg ccggagttcg
     1861 tcaaccgcat cgatgagatc gtagtgttcc atgcgctcga cgagaagcac atcatgaaca
     1921 tcgcgcgtat ccagatgcgc gggctggaag agcgtctggc gaagctggag atgaagcttc
     1981 agatcaccga cgaggcgctg gaggaactgg ccaaggtggg cttcgacccg gtgtacggcg
     2041 cgcggccgct caagcgcgcg atccagcagg agatcgagaa cccgctgtcg aagctcattc
     2101 tcgaaggccg cttcgctgcg aaggacacca tcgtggtgga gtatcgcgac aaccagatgc
     2161 gcttccgcaa agcgcagcct gcggagcggg aggcggcgac cggccgctga gtccaggcgc
     2221 tgatcagggg ctttaggcgc ggcgcccgcc gggcctaaag cccgttcagc gtgaacggcg
     2281 cgaacgccgg atcgagcacg gcgctcacct gtcgcgcctc gcgcaccagt tcctcccaca
     2341 ggccgtccat atccttgcgg aacacgacct cggggtccgg cggcatcaca taccagaggc
     2401 cgcggcggat ttcctgccgc aattcgcccg gccgccagcc gacgtgtccg atgtagtagc
     2461 gcgcatcgtt tggccgttcc tcgatgatct tgtcgatggt ctgcacaccc acggcgagaa
     2521 acacgccggg catcatgatg atcgaacccg gaccggggct ttgctcggtg tgcacggccg
     2581 cggtgagagc ggtgatgccg aaggggccgc cgaagaagac cggttccacc actttcttcg
     2641 aggggccgtg ctcgggaaac aggctgccca ggctgcgctg ggtcggccgg ttgagaatga
     2701 ttccgacgtg tccgccgcct tcggtaatgg tcgccagcag caccgtttgg cggtattccg
     2761 cgtcgaccag agcgggcgtg gccaccagca ccaccgcgtg taaatccttc acttcaccgg
     2821 ggctttgcgc atgcaccggc aacgcaccga tgaacatgca gatcagcgct gtcaccgtct
     2881 tcgtcagcag gttcatcctg gctctccccg cgtcgtacat cggccggcgc gcatcgaagc
     2941 cgtagcaaag cccagacccg ccgcgattga cgctggcggc gcttttgtgc aaggctgccc
     3001 ccgtgagcaa tgtcagcggc gtcgacgcag gaataacgga ggcacgcgat gtacaaaaaa
     3061 atattgatgg cctacaacgg cacgcaggag gggcgtaccg cactgttgac gtgcgccgaa
     3121 ctggcggcct tcgtccatgc cgagacgcat ctcctgtcgg tggcgacaat gccgtcgagc
     3181 atgttcctca ccgaaggttt cgtccccgag gaactgatcg acgaggaaaa gaagcgcatg
     3241 caggcggtgc tcgacgaggg cctcgaactg ctagcgcagc gtggcttcag cgttaccggg
     3301 catctggcgg tcggtgagcc ggtggaggaa atcagccggc tggcacgcga gctcgactgc
     3361 gacctcatca tcctcggcca caagcagcag cattcctttg ccgcgcgctg gtggcgcggt
     3421 tcggtcggcg cgacgctgct ggacgtctcg ccctgcagca tcctgctcac cgttaccgac
     3481 gggacagcca aaaactaaaa aacctcacca cagaggcaca gagacacaga gaaaagcaaa
     3541 gtttctgaag tggataggca ggcatccacc cccttcgatt gccgtggccg gctcgaccgc
     3601 ctggtcacga ccgacgcact cgcgtgctgt tcatctttga gacgatctat aggcgtgaac
     3661 aagagtagtg gctaaaccgg gacgtttttt aaggtttgct ctgtgcctct gtgtctctgt
     3721 ggttcaagcc ttcgggtttt agcgccgcac ggtgaaatcc acgcgggtgg tcgcgccgtc
     3781 gccggtgacg cctttggcat cgagtttggg tgcggcgacg tagacccgtt cgggcggcac
     3841 ctgtccctgc tcgcgcagcc aggtcaccac cgcctcggcg cgccggttgg ccagacggcg
     3901 catggcattc tcgttcaccg gcgtattcgc cagcagcagc ttttccattt ccgccggcgg
     3961 cagacgcttg gcgaaaccga ggaaattgcg cggcttgtcg aagcgcccgg catcgtaggc
     4021 ggccttcaga tacttctcgt attcctccgg ctcgatctcc acctcctcga gccgcggcgg
     4081 gttatccagt tcttcggtga gtgccttgag cttggccgtg cgcaggcgcc gcatcatcga
     4141 ttcgcggcgc agcgcatctt cctccgcctg cggatcgacg cgccctgcga catccagcgt
     4201 gaggccgggg cggttgcgca tcgcgcgggc aagccggctc aaccgctgct gcgccgcttc
     4261 gctcaattcg gcggagcccg gctcgaaagc gatgaaactc atgcgctcgc catcgccgcc
     4321 gaacatcgcg cccagcgcgg tgaagggcga ggtgacgacc ttgatgaaaa gattgccgat
     4381 ggccttggcg atcaccccgc cgatgctgaa ctgcggatcg ttgagcgagc cgctgatcgg
     4441 cacgtccaga tcgatcacgc cgttgctgtc cttcagcagc gagaccgcca gatgcaccgg
     4501 cacgttgagc gcctcggggc tttcgacctt ctcgccgaaa acgagctggt tgagcacgag
     4561 cttgttctgc gccgtcagct gatcgttttc gatgtggtac ttcacatcca tgctcagctt
     4621 gccgcgctcg atggggtagc cgatatagcg tacggaataa ggcgacagcg gcggcaggtt
     4681 gacgcccttg gccgacgcgt cgatatcgag aaacaggctt tggccgagcg ggttgatcag
     4741 gccggagatg gtgacaggcg cgatgtcgtc gatcgtgcct gtcagttcga tgcgtccggc
     4801 ctgctcgggc gacagcgcac cgatgctgcc ttccagcttg ccgatttccg cgcggtagtt
     4861 cggtttgatg tagcggtcgc tgaaatccag ctcgccgttg gccaggcgca cttcgccgat
     4921 ttcgatacgc ggcttgccgc gccgctcgat ggccggcttg ggtgcggccg tctcttccgg
     4981 cgcagcggtg tcgtccgctg tcgcagcatc gtccgatgcg gtgacctcgt tggcggcgac
     5041 cgtctcgccc ggcaccgtct gctgcgccgc ttcgtccttc gcatcgctcg gcgccgcgag
     5101 gacgtgctgc aggttcagcg tgccgtccgg cagcaggatg atgcgcgcaa aggaatcgtt
     5161 cagcgcgatg gcgcccacgc gcaccagcac cggctcggtg cgcagataaa cgtcctgcag
     5221 ccgtagccgc ttccagcgca gcaggtcgcg ccggctgccg ccttcgcgcg cgctgaaatc
     5281 gttcacgtcg atgcggccgc cgaagcgcag cgccgtctgt ccgcggcgat cttcacccag
     5341 gcgcagccgg ccgcgtgcgg caatcgcgcc gcgtgtgatg tcgagcgaaa tctgctcggt
     5401 ccagtagggc gagaagggca gcaccggaat cgcctgggcg ctgactttcc agtcgaagcc
     5461 gaagggcgcg agcttcatcg gtccgcccag gctcagcttg cccgccttct ggatggtggc
     5521 gttcagcgca agctgcggtt cggcatcggg cgcgctggac agtccgctga gcacgccgtt
     5581 gatgttgtgc gctttcagct tgaccggttg tttggttgcg cggtcctcgt actgcaggga
     5641 ggaggcctcc aggccgatgc gccggacttg ataggtccat gccggcccgt catccgccgc
     5701 gctggggttt ttctgcgctt gctcggctgc ttcggccgtt tcagcggcca catgcagcag
     5761 gccggtcacg taccagtcgc cgtttgcggt gcgtagcacc gtcgccgcca cgtcgcgcgt
     5821 gctcacctcg tccacgctta cgttgcgctt gcccacgtcg atgcgtacac cgctcacgtc
     5881 gatctggccg gcgcgccagc ggtccttgcc gatcgcggtg tggacgcgcg agtcgcgcac
     5941 cgtggccttc aacccctcca cgacgatatc gggtgctgcg ccgtccttca tcgccacgcg
     6001 ataggcgcct tcgccgctca gcgtgccgct gcccagttgg cgctccgggc cgaggtacgg
     6061 ctggaagtgg ggcagggcga gatcggcgag tttgaagttg ccgttcaccg cgacgggcgc
     6121 gagcgcgagc tggccctgcg tggtgaactg acctttgtcg gtgaggcgca gcgacacatc
     6181 gtatgtgccc tgttgttccg gatcgttgcc gatgttcttc gcgttcagtg cgatgtcggt
     6241 gaatgccgtt ctgaaaggct gcggcgcgtc gtagtcgagc agttgtacgc gcccgttggc
     6301 gatcgcgact tcatcgacgg cgtagcggaa cggctgcgca ggtttggatt ccgccgaagc
     6361 cgcggcgggc ttttgcgccg tgcccgtctc cagcggcatc agcagttcca gattcagcat
     6421 gccgccgcga aaacgcgtga cgtggacctg gggcgaggcg gcgcgtaccg cggcgagcga
     6481 caggcggttg ccgatcagat ccagttcgcg caattccacc gccagcgatt cgaaggcgat
     6541 catcggttcg tcgtcgcgct ggcgcacgtc gacgtcgcgt gccgtcgccg tgccggtcag
     6601 gctgagggtg gtcatgctgc ccggcgcgcc cgccatgcgc agcgtgagcc tcgcgtcgag
     6661 tgcgcccttg gcgagggcga agcgcagcgg aacgggcacg taatcgaaat actgcggcaa
     6721 ctgcacattg tcgaagttga tgtacagcag cgtctcccga tcctcgccga agggcttggt
     6781 ctcgccgctg acggccagcg gtgcgccgtt gaccaccgcg gacaggttcg gctggacgta
     6841 gatttccgtg gcgtagggca ggctggacag gaagggcacg ccgatctgca gttcgcgcac
     6901 ttcgtgccgc ttgccttcga gctgatcgtc gaagctgatc cgtccgtcga tcagctcgat
     6961 gttgttcagc gagaagcgcg gcggcgtctt gtcctcgggt tcgggcgcct ccggctgctg
     7021 ccagacgtaa cggtccacca cgtcctgcac gttgtattgc cggtcctcgt ggcgtaccag
     7081 tttgatgaag ggttcgcgga tgcgcagccg gtcgatgacc ggcgcaaggc ggaacagcga
     7141 ggtggaggac acattggcgt acgcttcgcc gataccggcg atggcctcgc cgccgtctgc
     7201 gtcgcgcacc tgcaggttgt cgatgcgcgc cgacagagag aaggggttgg tatacagccg
     7261 ctcgatgctg accggccgca tcagcgcctc gccgagcgcc tgttcgatgc gcggtttggc
     7321 gaaccagggc aggacgaaga agccgagcag ggcgtacagc gccaccagcc cgatcagcca
     7381 gacggccgcc cggcgcgttc gccggtgcgc gatggcggcg ccaaggcgct gccgaatcgt
     7441 actgaaggtc gacatggtgc gccgagctta gcagcggacg cgcgctttgg gggagcgcgg
     7501 cgctgtcagc ggacaggcgg gcgggtgaga ccggcttgcg ctgccggcga cggccgggcg
     7561 ctgcgattct cggtcagggc cagcagttgg ccgctgtatt cgcgcaggtt ggccaccgtc
     7621 gttcggcgtt gggcaggcgt gaggctgcgc tcgatggcga gcatcgtggc acggaaatcc
     7681 gcttcccagg cgcgcagcgc ttcgcggtat tgcggcagcg aattgcgctc ccaatgcacg
     7741 atcacctccc gcagtgccgc ttccgcctgc gcttcgccgc gtaccagcgc atccacagcg
     7801 gccagtgtgc gcgcctgcag gtgccggcgg ttttccagcc gcagttcggc aaagtgcggg
     7861 tgtcgtgcgg cgaattcggt cagcagccgc gtctgcgtgg cattcagatc gcccacccag
     7921 cgttccacat gatcggtcag cgattccacc atcgcctcga gccgctctgc ttcgttgccg
     7981 tcgagaaact cgtcggcata gtcttcgttg cgttccttca cgtacctgcg cagcgccgtg
     8041 aagttgcgcg cgtcgagggt gcgcaggatg gggatggctt cgtccaccgc ctgcgccgcc
     8101 aggcgctcgt atcgcgcctg ggccagctcg cgcgtctgcg cgatatcggc cgcgtcgagc
     8161 cccgcttcca cacggaccgc aagccggtcg agcatttcgg cataggcggg cagttcattg
     8221 tgccggtgcc agtcgtgcag gcgccgcaaa gcggcggaca tcatgacgtc ctgttcgtcg
     8281 ttcggatcga aggcgttgta cagcttgtag cgcagcacca ggggcgcgct gttgtacgcc
     8341 agttgcggtg ccgagcaggc gcccagcacc agcagaagga atccggcgca ggcgaggctc
     8401 agtgcgccgc gtgcgatgag agtgtgcatg cctgcagtgt cggcaagcgc catgccgcgg
     8461 catgacgctt gcgtcgggcg gggcgggcat tgcaagagga ttcggacatt ggcttccacc
     8521 ggccagcgac tcaaggggca tgacggcacg gcaagcaccg cgctgctgct gatcgacttc
     8581 atcaacgatc tggatttcga cggcggcgag caactcgcac cgcaggccgt ggccgccgcg
     8641 caggcgaccg cggcgctgcg cgcgcgcgcc gatgcggcga gtgtgccggt catctacgtc
     8701 aacgacaact tcggacgctg gcaatcgcgc ttcgatcagg cggtggcgca ttgccggcgt
     8761 tcgggcggac gcggccgcaa gctggcggaa ctgctgaccc cgcgagagca cgactacttc
     8821 attctcaagc ccatgcattc ggg
//
LOCUS       DATGOU010000034        18307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_522810, whole genome shotgun sequence.
ACCESSION   DATGOU010000034 DATGOU010000000
VERSION     DATGOU010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18307
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522810"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VKZ48_01885"
     CDS             <1..780
                     /locus_tag="VKZ48_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLU76637.1"
                     /translation="GRNDVMVPLSSLVSVEETVAPRELNHFNQRRSATITATLAPGYS
                     LGEALDYLEELARQELPVGYALDYNGNSREFKVSSGGLWFTFALALLFIYLVLSAQFE
                     SFRDPFIIMLTVPLSMTGALLALKLSGGTLNVYSQIGLVTLVGLITKHGILIVEFANQ
                     LQEKGETLLDAVVDAAVLRLRPIMMTTGAMVLGAVPLAFAHGAGAESRQPIGWVIVGG
                     LLLGTLLTLFVVPVVYSLLARRHRPAQEEPGAALDHAAQPH"
     gene            869..1591
                     /gene="dnaQ"
                     /locus_tag="VKZ48_01890"
     CDS             869..1591
                     /gene="dnaQ"
                     /locus_tag="VKZ48_01890"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HLU76638.1"
                     /translation="MRQIVLDTETTGLNARLGDRIIEIGCVELVNRRLTGKHFHRYIN
                     PGRAVEEGALRVHGITDEFLADKPRFEEVVEEFVEYVRGAELIIHNAAFDVEFLDMEL
                     EKCRRGRLEQCVAGVIDTLRHARELHPGKKNSLDALCERYAIDNAKRTLHGALLDAEL
                     LAEVYLAMTRGQESLGIDLEETRDERATGATPLRPRPALIVLAATAEEEAEHAAVLEA
                     IAKEAKGTAIWQRAASQEASVA"
     gene            1732..3381
                     /locus_tag="VKZ48_01895"
     CDS             1732..3381
                     /locus_tag="VKZ48_01895"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HLU76639.1"
                     /translation="MTKYVFVSGGVVSSLGKGIAAASLAAILESRGIKVTLLKLDPYI
                     NVDPGTMSPFQHGEVFVTEDGAETDLDLGHYERYISAKMRKVNNFTTGQIYESVIKKE
                     RRGDYLGRTVQVIPHITDEIKSFIERGAGDADVAIVEIGGTVGDIESLPFLEAVRQMR
                     LELGDRNTCYLHLTLVPYIKSAGEIKTKPTQHSVKELREIGIQPDAVLCRTEAPLPED
                     ERKKIALFCNLSEKAVISVADADSIYKIPAMLHQQMLDEIVCHKLDLLAKPANLSVWD
                     KLVHALENPKHQVTIALVGKYVELTESYKSLSEALTHAGIHTRAKVDIRYIDSEDIER
                     DGVGELKNVDAILVPGGFGKRGVEGKIQAIQYARENNVPYLGICLGMQLAVIEYARHK
                     CGLDGANSTEFDSETPHPVVALITEWMGRDGKVEKRDEQSDLGGSMRLGAQAAVLEPG
                     SRAREIYGKDVINERHRHRYEVNDFYLPQLKQAGLRIAGLTEGEKLVEMVELPDHPWF
                     IGCQFHPEFTSTPRDGHPLFKSFIEAALEHQARAGNKAATV"
     gene            3384..4241
                     /gene="kdsA"
                     /locus_tag="VKZ48_01900"
     CDS             3384..4241
                     /gene="kdsA"
                     /locus_tag="VKZ48_01900"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HLU76640.1"
                     /translation="MKLCGFEVGLDRPIFLIAGPCVVESREMAHDTAGRLKEIAAAVG
                     VPFIYKSSYDKANRSSGKSFRGPGMDKGLEILADVRKKIGVPVITDVHTEAEVPAVAE
                     AVDVLQTPAFLCRQTDFIRAVAQSGKPVNIKKGQFLAPGDMKNVVDKAREAAAEVGLK
                     DNILVCERGVSFGYNNLVSDMRALAIMRETGCPVVFDATHSVQLPGGQGTSSGGQREF
                     VPVLARAAVASGIAGLFMETHPDPAKALSDGPNAWPLDRMQELLETLKGLDELVKRSG
                     FAEQTLAGH"
     gene            4308..5591
                     /gene="eno"
                     /locus_tag="VKZ48_01905"
     CDS             4308..5591
                     /gene="eno"
                     /locus_tag="VKZ48_01905"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HLU76641.1"
                     /translation="MSSIVDVVAREILDSRGNPTVEADVLLESGVMGRAAVPSGASTG
                     SREALELRDGDKQRYLGKGVLQAVEHINTEISEVIMGLDASEQSFIDKTLIELDGTEN
                     KGRLGANALLAVSMAVAKAAAEEAGLPLYRYFGGSAPMQMPVPMMNVINGGAHANNSL
                     DMQEFMILPIGAKSFREALRCGAEIFHALKKLLDQKGMSTTVGDEGGFAPNLGSNAEA
                     LELIMQAIDNAGYMAGGDVVLGMDCASSEFFKDGRYHLSSDGLQLDSSEFADYLATLC
                     DRFPIISIEDGMAEGDWDGWQILTERLGQRVQLVGDDLFVTNTKILREGIKLGVANSI
                     LIKVNQIGTLSETFAAIEMAKRAGYTAVISHRSGETEDTTIADIAVGTNAMQIKTGSL
                     SRSDRVAKYNQLLRIEEDLGDAVSYPGREAFYQLR"
     gene            5630..5929
                     /gene="ftsB"
                     /locus_tag="VKZ48_01910"
     CDS             5630..5929
                     /gene="ftsB"
                     /locus_tag="VKZ48_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HLU76642.1"
                     /translation="MKILAIVLGALIVLIQYPLWLGKGGWLKVWEADRQVATQVEQND
                     ALQSRNDALAAEVSDLKQGMEAVEERARQDLGFVKRDEVFFQIVEPPKDPNAPKP"
     gene            5956..7296
                     /locus_tag="VKZ48_01915"
     CDS             5956..7296
                     /locus_tag="VKZ48_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015038811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HLU76643.1"
                     /translation="MNLHRLLVEREAGGRPLRVALIGAGKFGAMYLSQARTTPGMHIA
                     AVVDLQPALARDALTRTHWEAGRIDARSIEQAVKSRTVYITDDAMAVIASPHIDIVID
                     ATGHPAAGIKHVLACCEHGKHIVMVNVEADALAGPLLALRAQEAGVVYSLAYGDQPAL
                     ICELVDWARTAGLDVVAAGKGTKYLPEYHASTPDTVWPHYGFTPEMVAQGDFNAQMFN
                     SFLDGTKSAIEMAAVANATGLQPAPHGLEFPPCGVDDLARVLRPRDVGGVLHHSGQVE
                     VVSSLNRDGTPVFRDLRWGVYVVFEGGTDYVHKCFREYGLITDDTGRYSAMYKPYHLI
                     GLELGISVASVGVRKEPTGAPQGWNGDVVATAKRDLQAGETLDGEGGYTVYGKLMPAA
                     DSLRCGGLPLGLAHGVKLVQPVKAGAPVRWSDVTIDENDAAVRFRREMERVFAA"
     gene            7724..8065
                     /locus_tag="VKZ48_01920"
     CDS             7724..8065
                     /locus_tag="VKZ48_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76644.1"
                     /translation="MSVSIAPSIKAEPYVRLRAWMLLEDGRGHRYFVGRCEDTRQSRI
                     SSAIRYFDADKRRGTTFSGRVYALSGEAGIDAETQRVLERWLELNRIVEWKDVSAELP
                     GFIATSIPPVD"
     gene            8178..9368
                     /gene="chrA"
                     /locus_tag="VKZ48_01925"
     CDS             8178..9368
                     /gene="chrA"
                     /locus_tag="VKZ48_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HLU76645.1"
                     /translation="MSPHGAFASAEVFRAFLKLGLTAFGGPIAHLGYFREEFVQRRRW
                     LDEAAYAELVALCQFLPGPASSQVGFALGLMRGGVSGALLAWLAFTVPSALFMLLVAY
                     GADGYGGPVGQLIVRGLKLVAVAIVAHALWGMARNLCPDVQRAVIAALAAVALLMLPG
                     AAAQVSAIAAGALAGMWICRHVSARETGSLQVAVSRRTGAFALASFFVLLVGLPLLVT
                     AWPQAYAQLFEAFYRAGALVFGGGHVVLPLLEARVVGQGWTTPDVFLTGYGAAQAVPG
                     PLFSFAAYLGAVVASQPLIGAALALVGIFLPGFLLLVGVLPFWNALRERPWAYAAIAG
                     ANAAVVGVLAAALYRPVWTSAIADIADVAIAAIGFLLLVALRLPPWSVVLLVVAATVG
                     VRMI"
     gene            complement(9365..10588)
                     /locus_tag="VKZ48_01930"
     CDS             complement(9365..10588)
                     /locus_tag="VKZ48_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HLU76646.1"
                     /translation="MKARVVVVGGGPAGLMAAETLSQAGVAVELFDAMRSVGRKFLLA
                     GRGGLNLTHSEPRAAFDARYGARRGEIAAMLDAFGPEQLRAWAHALGGETFIGSSGRV
                     FPRAMKAAPLLRAWLHRLRESGVRIHVRHRWIGWSDDGSALRFDTPAGETQCKVDAVV
                     LALGGGSWAKLGSDGAWLPTLQRHGVDVAPLRPANCGFELQWSAHFRERHAGQPVKAV
                     VLRHGTLERTGEFVISDYGAEGSAIYALSASLRDAIERDGETLLQLDLAPQRTHEWLV
                     RQIAHPRGSRSMSSHLRSRIGLEGVKAGLLRECAPPETYNDPVALAHAIKALPLRLLA
                     PRPIDEAISSAGGVRFEALDEQLMMRALPGVFCAGEMLDWEAPTGGYLLTGCFASGRA
                     AAMGTLHWLRTRAAR"
     gene            10897..13353
                     /locus_tag="VKZ48_01935"
     CDS             10897..13353
                     /locus_tag="VKZ48_01935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HLU76647.1"
                     /translation="MAVAATSDIRETPSCFHCGLPADGDGGRRTLVGGEWRDFCCPGC
                     EAVASTILDQGLADYYRLREMAPARPEDVEDISPVYDDPTFQASFVRVHPDGTSEASL
                     LLEGVRCAACVWLIEETLKRVEGVQSAEVNYVTRRARLRWNPAQTPLSGVLRRIRAIG
                     YGAHPYQPDRAELLRKAEQRQWLWRLFVAGFGMMQVMMYAFPVYVAGDGTMTQDIEQM
                     MRWASLVLTVPVVGYSAQPFFRGAWRELRTRRFGMDFPVALGIAVGFGASTWATITAS
                     GDVWFDSVTMFIAVLLLGRYLELRARARVSDALSHLARLVPAAGERLLDYPRTREVER
                     IAVAHLAAGDYVLVKPGEPVPADGVVVEGHSEVNESMLTGESRPVSKTVGSALVGGAI
                     NVASPLVMRVERVGGDTMLSSIVRLVERASTERQPLAELADRYAHWFVILVLAVAAGT
                     AVAWSFIDSERALWITVSVLVATCPCALSIATPVALTAATGELARRGFVATRGHVVET
                     LARATDVVFDKTGTLTEGEPRVLNVRLYAGMTRERAAAIAAALESGSEHPIARAIERY
                     AADSTQTSLTADALANTPGEGVEGEVGGRRYRLGRRAFAAAPSGWLGAEVEATRHTEA
                     WLCDDSGPLACFELGDRLKPQANELVESLRAAGKRVHLLSGDGAGAVHEAARALGIEH
                     VRADATPEEKGAYVQALQREGAVVAMVGDGINDAPVLAQADVSIAMASGATLAQTHAD
                     AVLLSGQPQDLMRALRLTERGRDILRQNLAWGFGYNLLVIPAAVVGLVSPWLAALGMS
                     ASSLLVVCNALRLRRARPEY"
     gene            13356..13523
                     /gene="ccoS"
                     /locus_tag="VKZ48_01940"
     CDS             13356..13523
                     /gene="ccoS"
                     /locus_tag="VKZ48_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HLU76648.1"
                     /translation="MDVLYLLIPLSLVLVFLIAVVFWRALKSGQFDDLEGPGFKILLD
                     DDRPSDTPPSQ"
     gene            13693..15126
                     /gene="ccoN"
                     /locus_tag="VKZ48_01945"
     CDS             13693..15126
                     /gene="ccoN"
                     /locus_tag="VKZ48_01945"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HLU76649.1"
                     /translation="MDAVNTTYNYKVVKQFAIMTVIWGIVGMFVGVVVATQFVFPDLT
                     HGIPWLSFGRLRPLHTNAVIFAFGGCGLFATSYYVVQRTCHVRLFSDRLASFTFWGYQ
                     AVILLAVITLPLGITSSKEYAELEWPIDILLAIVWVCYGIVFFGTIAKRKVPHIYVAN
                     WFFGGFILAVALLHIVNSMALPATLTKSYSAYAGVQDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAERPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSVGMIFSL
                     ILLAPSWGGMINGIMTLSGAWHKLRTDPILKFLIVSLSFYGMSTFEGPMMAIKTVNAL
                     SHYTDWTIGHVHAGALGWVGFITIGSLYYLIPRMYGRTEMWSTKLITVHFWIATIGVV
                     LYIAAMWISGVTQGLMWRAVNDDGTLTYTFVESTKASMPFYAIRLLGGLMYLSGMIMM
                     FYNLLKTAAIGRQVDARIPAVEAPAHA"
     gene            15145..15759
                     /gene="ccoO"
                     /locus_tag="VKZ48_01950"
     CDS             15145..15759
                     /gene="ccoO"
                     /locus_tag="VKZ48_01950"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HLU76650.1"
                     /translation="MALTQEKIERNVALLIIVTVLVVSVGGLVEIVPLFFQKSTTEPV
                     AGLKPPTAVQLAGRDIYIREGCNNCHSQMIRPFRAETERYGHYSVAGEFVYDRPFLWG
                     SKRTGPDLARVGGRYSDDWHRIHLINPRDVVPESIMPAYPWLENTPVYSGDIEARMKT
                     LRKLGLPYTDEDIAGGKKMLEGKTEMDVLIAYLQSLGLALKDRK"
     gene            15767..15925
                     /locus_tag="VKZ48_01955"
     CDS             15767..15925
                     /locus_tag="VKZ48_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HLU76651.1"
                     /translation="MEWLNDLRVLITVLSFLSFLGIVFWAYRPKSQAGFERAAQSVLE
                     EEEPGGQQ"
     gene            15922..16902
                     /gene="ccoP"
                     /locus_tag="VKZ48_01960"
     CDS             15922..16902
                     /gene="ccoP"
                     /locus_tag="VKZ48_01960"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HLU76652.1"
                     /translation="MTDFVNGFWEFYVGIITLAGVIGCWLLLQSQNKVKAKKNQQVET
                     TGHVWDGDLAELNNPLPNWWRWMFYLTIIFSLGYLVLYPGLGFFDGTLGWSSVQRYEN
                     EQTKAEEQYGPLYAKYTQMSVEEVAADPAAIAMGQNLYLNYCAQCHASDARGSKGFPN
                     LADRDWLWGGDPKTITQTIMQGRTGMMPPMGPALGSEGTRDVAHFVRSLSGLTHDSIR
                     ATRGRPLFAQNCAVCHGADGTGNQMLGAPNLTDRTWLYGSSEATIIETITKGRNNVMP
                     AWKEFLGESKIHVLAAYVWSLSNEPAQRDPLAEPMKTGPEQTEPVKVGQN"
     gene            16994..>18307
                     /gene="ccoG"
                     /locus_tag="VKZ48_01965"
     CDS             16994..>18307
                     /gene="ccoG"
                     /locus_tag="VKZ48_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HLU76653.1"
                     /translation="MEDIPVVQVGAIDEVEQSLYEVRKHIYVRAVHGWFARWRWIFVW
                     LTQIVFYGLAWMEWNGRQAVLFDLAARKFYIFGYVFWPQDFIYLTALLIISAYSLFLF
                     TAIGGRLWCGYACPQTVYTEIFMWIERKIEGDRQRRMRLDAQPMSPRKFALKTAKHGA
                     WIAVALWTGFTFVGYFTPIRELGGDILTLDIGPWATFWLLFYGFATYGNAGWMREQVC
                     KYMCPYARFQGVMFDKDTMVVTYDTARGEPRGARGRKADPKAQGLGDCIDCTMCVQVC
                     PTGIDIRDGLQYECIGCAACIDACDTVMDKMGYARGLIRYSTENAMAQGWNFGQMVRR
                     VFRPRVLIYSAILLAIISVTVYSLYARVPLKVDVLRDRASLMREVEDGLLENVYRLQI
                     MNTQEVPHMYRITVTGLESLHVASDDVFELPAAGSRQVPLRLRMDG"
BASE COUNT         3208 a         5700 c         6007 g         3392 t
ORIGIN      
        1 ggccgcaacg acgtgatggt gccgctgtcc agtctggtat cggtggagga gacggtggcg
       61 ccgcgcgaac tgaaccactt caaccagcgc cgttcggcga ccatcaccgc cacgcttgcg
      121 cccggctatt cgctgggcga ggcgctcgat tatctggaag agctggcgcg gcaggaattg
      181 cccgtcggct acgcgctgga ctacaacggc aactcccgcg agttcaaggt gtcctcgggc
      241 ggcctgtggt tcaccttcgc gctggcgctg ctgttcatct atctggtgct gtccgcgcag
      301 ttcgaaagct tccgcgatcc tttcatcatc atgctgaccg tgccgctttc catgaccggc
      361 gcgctgctcg ccctcaagct ctcgggcggc acattgaacg tttacagcca gatcggcctg
      421 gtcacactgg tcggcctcat caccaagcac gggatcctga tcgtggaatt cgccaaccag
      481 ttgcaggaga agggcgagac gctgctcgat gcggtcgtcg atgcggccgt gctgcgtctg
      541 cgcccgatca tgatgaccac cggcgcgatg gtgttgggcg ccgtgccgct tgctttcgcg
      601 cacggcgcgg gggcggaaag ccgccagccg atcggctggg tcatcgtcgg cggattgctg
      661 ctcggcacct tgcttaccct ctttgtcgtg ccggtggtgt acagtctgtt ggcgcgccgg
      721 catcgtccgg cgcaggaaga gcccggcgct gcgctggatc acgcggcgca accgcactga
      781 tcacagcagg tccatgcggg cgggcattcc cggcatggcc gggacttccg cgcggaaatg
      841 actagaaaaa ttacgccagg gatttgccat gcgtcagatc gtcctcgata ccgaaaccac
      901 cggcctcaac gcccgtctgg gcgaccgcat catcgagatc ggctgtgtcg aactggtgaa
      961 ccgccgcctg accggcaagc actttcaccg ctacatcaat ccgggacgcg cggtggaaga
     1021 gggcgcactg cgggtgcacg gcatcaccga cgaattcctc gccgacaagc cccgcttcga
     1081 ggaagtggtg gaggaatttg tcgagtatgt gcgcggcgcc gaactgatca ttcataacgc
     1141 cgccttcgac gtcgagttcc tcgatatgga actggaaaaa tgccggcgag gccggctgga
     1201 acagtgtgtt gccggcgtga tcgacacact gcgccatgcg cgcgaactgc atccgggcaa
     1261 gaagaattcg ctcgatgcgc tgtgcgagcg ctatgccatc gacaacgcga agcgcactct
     1321 gcatggcgcc ttgctcgacg cggaattgct ggccgaggtc tatctggcca tgacgcgcgg
     1381 gcaggagagc ctcggcatcg atctggagga gacgcgcgac gagcgcgcga cgggtgcgac
     1441 gccgctgcgc ccgcgtccgg cgctcatcgt ccttgccgcc acggcggagg aggaggccga
     1501 acacgcagcc gtgctggaag ccatcgccaa ggaagccaag ggcaccgcga tctggcagcg
     1561 tgcggcgagc caggaggcga gcgtggcctg atcgccgttc gctcggcgcg ttgattcgcc
     1621 gggtgttttg cgcgcagccg gcagcacgga tgccggccat gcaacacgcc gtcaaattga
     1681 gccggcgggg cgtttcggct atcattcgcc gtccgaagcc caacttccgc aatgaccaaa
     1741 tatgtattcg tctcgggcgg tgtggtgtcc tcgctgggca agggaatcgc agccgcttcg
     1801 ctagccgcga ttcttgagtc gcgcggcatc aaagtcaccc tcctcaagct cgacccctac
     1861 atcaacgtcg atcccggcac catgagccct ttccagcatg gagaggtgtt cgtgaccgag
     1921 gacggcgccg aaaccgacct cgatctgggc cattacgagc gctacatctc ggccaagatg
     1981 cgcaaggtga acaacttcac caccggccag atctacgaaa gcgtgatcaa gaaggagcgc
     2041 cgcggcgact acctcggccg cacggtgcag gtgattccgc acatcaccga cgagatcaag
     2101 agtttcatcg agcgcggtgc gggcgatgcc gatgtggcca tcgtcgaaat cggcggcacc
     2161 gtgggcgaca tcgaatcgct gccgttcctg gaggccgtgc ggcagatgcg cctggaactg
     2221 ggcgatcgca acacctgtta tctgcacctc acgctggtgc cgtacatcaa gtccgccggc
     2281 gaaatcaaaa ccaagccgac ccagcactcg gtcaaggaac tgcgcgaaat cggaatacag
     2341 cccgacgccg tgctgtgtcg caccgaggcg cccttgcccg aggacgagcg gaagaagatc
     2401 gcgctgttct gcaacctgtc ggaaaaggcg gtgatttccg ttgcggacgc cgattcgatc
     2461 tacaagattc cggccatgct gcatcagcag atgctcgacg agatcgtctg tcacaagctc
     2521 gatctgctgg ccaagccggc caatctgtcc gtctgggaca aactggtgca cgcgctggag
     2581 aacccgaagc atcaggtgac catcgcgctg gtcggcaagt acgtcgagct gaccgagtcc
     2641 tacaaatcgc tgtccgaagc gctcacccat gcgggcatcc atacccgggc caaggtggac
     2701 atccgctaca tcgattcgga agacatcgaa cgcgacggcg tcggcgaatt gaagaatgtc
     2761 gacgcgatcc tggtgcccgg cggtttcggc aagcgcggcg tcgaaggcaa aatccaggcg
     2821 atccagtacg cgcgcgagaa caatgtgccg tacctgggca tctgtctcgg tatgcaactc
     2881 gccgtcatcg aatacgcgcg ccacaagtgc gggctggacg gggcgaactc cacggagttc
     2941 gattccgaga cgccgcatcc ggtggtggca ctcattaccg aatggatggg gcgcgacggc
     3001 aaggtggaga aacgcgacga gcaatccgat ctgggcggca gcatgcgcct cggtgcgcag
     3061 gcggcggtgc tcgaacccgg cagccgtgcg cgcgagatct acggcaagga cgtcatcaac
     3121 gagcggcacc gtcatcgcta tgaggtgaac gacttctatc tgccgcaact gaagcaggcg
     3181 ggtctgcgga ttgccggcct caccgaaggc gagaagctgg tcgaaatggt agagctgccc
     3241 gatcacccct ggttcatcgg ctgccagttc caccctgaat tcacctccac gccgcgcgac
     3301 ggccatccgc tgttcaagag cttcatcgaa gcagccctcg aacatcaggc ccgtgccggc
     3361 aacaaggcag cgacggtata gccatgaaac tttgcggctt tgaagtcggg ctcgatcggc
     3421 cgatattcct gatcgcgggc ccttgtgtcg tcgagtcgcg cgaaatggcg cacgacaccg
     3481 ccggccgcct gaaggagatt gcggccgccg tcggcgtccc ctttatttac aagtcttcct
     3541 acgacaaggc caaccgcagc tccggcaaat cctttcgcgg gccgggcatg gacaagggcc
     3601 tggaaatcct ggccgacgtg cgcaagaaaa tcggcgtacc ggtgatcacc gacgtgcaca
     3661 ccgaagccga agtgcccgcc gtggccgaag cggtggacgt gctgcagacg ccggccttcc
     3721 tgtgccggca aacggatttc atccgcgccg tggcgcaaag cggcaagccg gtgaacatca
     3781 agaagggcca gtttctcgcc ccgggcgaca tgaagaacgt ggtcgacaag gcgcgcgagg
     3841 ccgcggcgga agtcgggctg aaagacaata tcctggtgtg cgagcgcggc gtctcgttcg
     3901 gttacaacaa cctggtgtcg gacatgcgcg cgttggcgat catgcgcgag acgggctgcc
     3961 cggtggtgtt cgatgcgacg cattcggtgc aactgcccgg cgggcagggc acttcgagcg
     4021 gcggccagcg cgaattcgtg ccggtgcttg cgcgtgcggc ggtggcgagc ggcatcgccg
     4081 ggctgttcat ggaaacgcat cctgatccgg ccaaggcgct gtccgacggg ccgaacgctt
     4141 ggccgctcga ccgcatgcag gaattgctgg agacgctgaa gggactggat gaactggtca
     4201 agcgcagcgg tttcgccgaa cagacgctgg ccggtcactg agccgtcgcg agtggcctga
     4261 agattttcaa gcattaacag catcaagcta aacaatcaga ggttctcatg agttctatcg
     4321 tcgacgtcgt cgcaagagaa atcctcgatt cgcggggcaa tcccacggtc gaggccgatg
     4381 tgctgctgga atccggcgtc atggggcggg cggcagtgcc ctcgggcgct tccacgggct
     4441 cgcgcgaagc gctggaactg cgcgacggcg acaaacagcg ctatctcggc aagggcgtgc
     4501 ttcaagcggt ggagcacatc aacactgaga tttccgaagt catcatgggg ctcgacgctt
     4561 ccgagcagag cttcatcgac aagacgctga tcgaactgga cggcaccgag aacaaggggc
     4621 gtctgggcgc caacgcgctg ctggccgtgt ccatggccgt ggcgaaggcc gcggccgaag
     4681 aagcggggtt gccgctttac cgttacttcg gcggctcggc gccgatgcag atgccggtgc
     4741 cgatgatgaa cgtcatcaac ggtggcgcgc acgccaacaa cagtctcgac atgcaggagt
     4801 tcatgatcct gcccatcggc gcgaagagct tccgcgaggc gctgcgctgc ggcgccgaaa
     4861 tcttccacgc gctgaagaaa ctgctcgatc agaagggcat gtccaccacg gtgggcgacg
     4921 aaggcggttt tgcgcccaat ctcggttcca acgccgaggc actggaactc atcatgcagg
     4981 ccatcgacaa cgcgggctac atggccggcg gcgacgtcgt gctcggcatg gactgcgcga
     5041 gttcggaatt cttcaaggac ggccgctatc atctgagctc cgacggcctg caactcgatt
     5101 cctcggagtt cgccgactat ctggcgacgc tgtgcgacag gttcccgatc atttcgatcg
     5161 aggacggcat ggccgagggc gactgggacg gctggcagat tctgacggag cgtctgggcc
     5221 agcgcgtgca actcgtgggc gacgatcttt tcgtcaccaa cacgaagatc ctgcgcgaag
     5281 ggatcaagct gggggtggcg aactccatcc tcatcaaggt caaccagatc ggcacgctga
     5341 gcgaaacctt cgccgccatc gaaatggcca agcgcgccgg ttacaccgcg gtgatttcgc
     5401 accgctccgg cgagaccgaa gacaccacca tcgccgatat cgccgtgggc accaacgcga
     5461 tgcagatcaa gaccggctcg ctgtcgcgtt cggaccgcgt cgccaagtac aaccagttgc
     5521 tgcgcatcga agaagatctg ggcgacgccg tgtcgtatcc gggccgcgag gcgttctacc
     5581 agttgcgttg aacacgaggg cggcattgcc gcccttcgtt ttaagtccca tgaaaatcct
     5641 ggccatcgta ctcggggcgt tgatcgtgct gattcagtac ccgctctggc tgggcaaggg
     5701 cggttggctc aaggtgtggg aggccgaccg ccaggtggca acgcaggtcg agcagaacga
     5761 cgccctgcag tcgcgcaacg acgcactggc tgcggaagtc tccgatctca agcagggtat
     5821 ggaagcggtc gaggagcgcg cacgccagga cctgggcttc gtcaagcgcg acgaagtgtt
     5881 cttccagatc gtcgagccgc cgaaggatcc gaatgcgccg aagccgtgag cggtgcgcca
     5941 tagcaggcaa tcgtcatgaa tctccatcga ctactcgttg agcgcgaggc cggaggacgc
     6001 ccgctgcgcg tcgcgcttat cggcgcgggc aagttcggcg ccatgtatct ctcgcaggcg
     6061 cgtaccacgc ccggcatgca catcgcggcg gtggtcgatc tccagccggc gctggcgcgc
     6121 gacgcgttga cgcgcacgca ctgggaagcc gggcgcatcg atgcgcggtc gatcgaacag
     6181 gcggtgaagt cgcgtaccgt atacatcacc gacgatgcca tggcggtcat cgcgtcgccg
     6241 catatcgaca tcgtgatcga cgcgacgggg catcccgccg caggcatcaa gcatgtactc
     6301 gcctgctgcg agcacggcaa gcacatcgtg atggtcaacg tcgaagccga cgcgcttgcc
     6361 gggccgctgc tcgcgcttcg cgcgcaggag gcgggcgtcg tctattccct tgcctacggc
     6421 gatcagccgg cgctgatttg cgaactcgtc gattgggcgc gcacggccgg cctcgatgtg
     6481 gtcgctgcgg gcaagggcac caaatacctg cccgagtacc acgcctccac gccggacacg
     6541 gtgtggccgc actacggctt cacgcccgag atggtcgcgc agggcgattt caatgcgcag
     6601 atgttcaaca gctttctcga cggcaccaag tcggccatcg aaatggcggc ggtggcgaat
     6661 gcgaccggcc tgcagcccgc gccgcacggc ctcgagtttc caccctgcgg cgtcgacgat
     6721 ctggcccgcg tgctgcggcc gcgagacgtc ggcggcgtgt tgcatcacag cggccaggtg
     6781 gaggtggttt ccagcctgaa tcgagatggg acgccagtgt tccgcgattt gcgctggggc
     6841 gtatacgtgg tgttcgaagg cggcaccgac tacgtgcaca aatgtttcag ggagtacggc
     6901 ctcattaccg acgacaccgg ccgttattcg gcgatgtaca agccctatca cctcatcggc
     6961 ctggaactgg gcatttcggt ggcatcggtc ggcgtgcgca aggaaccgac cggtgcgccg
     7021 cagggctgga acggcgacgt ggtcgcaact gcgaagcgcg atctgcaggc aggcgagacg
     7081 ctggacggcg agggcggcta taccgtgtac ggcaaactga tgccggcggc cgattcgctg
     7141 cgttgcggcg gcttgcctct gggactggcg cacggcgtca agctcgtgca accggtcaag
     7201 gcgggtgcgc ctgtgcgctg gtcggacgtc accatcgacg aaaacgatgc ggcggtacgc
     7261 ttccgtcgcg agatggagcg tgtcttcgcg gcgtgatcgt gcggcttgaa cgctgactgg
     7321 tgctcaacag cgctgaccgt aattcaacgt tttggcaccc tcccgtcggc gcacgaagta
     7381 cgcacaacgt cgtcgtcgcg catgcagcgt ccgcacggca ccggctttcc ccgtggcgca
     7441 tgcctaaaaa atcacactcc tgcgcacgct cacgtgctcg tgcatcgatg tggcgcacat
     7501 cctgcaatgg atggggcggg gcgctacagg gagagcgtat ttcgttcagg cattgggggc
     7561 tggacgaagc ccggggggta gaaaggccgc cgcgaggcgg cctttctttt atggcgtagc
     7621 tgccgtttgg attttccagt ccggcgcttg gagcgcctgc gatcaccggg tagcgacgcg
     7681 gtcgcaagat aaaaaagttc agaacaacga gagagggtca tcgatgtcgg tttccatcgc
     7741 tcccagcatc aaggccgagc cttatgtgag gctccgcgcg tggatgttgc tcgaagacgg
     7801 gcgtgggcac aggtatttcg tcggtcgttg cgaagacacg cggcagagtc ggatcagttc
     7861 tgcaattcgc tatttcgatg cagacaagcg tcgtggaacg actttcagcg gtcgtgttta
     7921 tgcgttgtcc ggggaagccg ggatcgacgc ggaaacgcag cgcgtgctcg aacgctggct
     7981 cgaattgaac cggatcgtcg aatggaagga cgtgtccgcc gaacttccgg ggttcatcgc
     8041 cacatcgatc ccgcccgtcg attgacgccc cttcccacgg gtggcgatcc gcagcggctc
     8101 gccttctgtg atcgctcgcc tcatgcgtca taatgcttgg cgcgccctgc gtacggcgca
     8161 agcagggagt gaaaaacatg tcgccgcacg gcgccttcgc atccgcggaa gtcttccgcg
     8221 cctttctcaa actggggctg accgcattcg gcggcccgat cgcgcacctg ggctatttcc
     8281 gcgaggaatt cgtccaacgg cgtcgctggc tggatgaagc cgcgtatgcg gaactggttg
     8341 cgctgtgcca gtttctgccc ggccccgcca gcagtcaggt cggtttcgcg ctcggtttaa
     8401 tgcgaggtgg cgtttcgggc gccttgctcg cctggctcgc gttcaccgtg ccgtccgcat
     8461 tgttcatgct gctcgtggcg tacggcgccg acgggtacgg cggccccgtg gggcagctga
     8521 tcgtgcgcgg attgaagctc gtcgccgtcg cgatcgtggc gcatgcgcta tgggggatgg
     8581 cgcgcaatct ttgtccagac gtgcaacgcg ccgtgatcgc agcgctcgcc gcggtcgcct
     8641 tgctcatgct gcccggcgcc gccgcgcaag tgtccgccat tgcggccggc gcgctcgccg
     8701 gcatgtggat ttgccggcat gtgtcggcgc gggagacggg gagtttgcaa gtcgcggtgt
     8761 cgcgccgcac cggcgcgttt gccctcgcgt ccttctttgt gctgctggtc ggattgccgt
     8821 tgctcgtcac agcgtggcca caggcgtacg cgcagttgtt cgaggccttt tatcgcgccg
     8881 gcgcactggt gttcggcggg ggccatgtcg tgttgccgtt actcgaggcg cgtgtggtgg
     8941 ggcagggctg gacgacgccc gatgtatttc tcaccggcta cggcgcagcg caggcggttc
     9001 ccggaccgtt gttcagcttt gccgcctacc tgggcgcggt ggtcgcatca cagcctctca
     9061 tcggcgccgc tctcgccctg gtcggcattt tcctgccggg ctttctgttg ctcgtcggcg
     9121 tactgccgtt ctggaacgct ttgcgcgagc gaccgtgggc gtatgctgct atcgccggcg
     9181 cgaacgcggc ggtcgtgggg gtgctggctg cggcactcta tcgtccggta tggaccagtg
     9241 cgattgccga tattgccgat gtggcgatcg ccgccatcgg cttcctgttg cttgtcgcct
     9301 tgagactgcc gccgtggtcg gtggtgctgc tcgtcgtcgc ggcgactgtc ggcgtgcgaa
     9361 tgatttagcg cgccgcccgc gtgcgcagcc agtgcagcgt gcccatggct gccgcgcggc
     9421 cgctggcaaa acagccggta agcagatagc cgccggtcgg cgcctcccag tccagcattt
     9481 cgccggcgca aaaaacgccg ggcagggcgc gcatcatcag ttgttcgtcg agcgcttcga
     9541 agcgcacgcc gcccgcgctg ctgatcgctt cgtcgatcgg ccgcggtgca aggagccgca
     9601 gcggcagcgc cttgatcgcg tgcgccagtg ccacgggatc gttgtaagtc tccggtgggg
     9661 cgcattcgcg cagcaggccg gcctttacgc cttccagacc gatccggctg cgcaagtgac
     9721 tcgacatgga gcgcgagccg cgtggatggg cgatctgccg gaccagccat tcatgcgtgc
     9781 gctggggcgc cagatcgagc tgcaaaaggg tttcgccatc gcgttcgatc gcgtcgcgca
     9841 acgatgcgga gagcgcgtag atggcgctgc cttcggcgcc ataatcgctg atcacgaatt
     9901 cgcccgtgcg ttccaacgtg ccgtgccgca gtaccaccgc ttttaccggt tggcccgcgt
     9961 ggcgttcgcg gaagtgtgcc gaccattgca gctcgaagcc gcagttggcg gggcgcaagg
    10021 gcgcaacatc gacgccatgt cgctgcagcg tgggcagcca cgcgccgtcg gagccgagct
    10081 tcgcccaact gccgccgccc agcgcaagta ccaccgcatc gaccttgcac tgcgtttcgc
    10141 ccgccggcgt atcgaagcgc aaagcgcttc cgtcgtcgct ccagccgatc cagcgatggc
    10201 gaacgtgaat gcgcacgccg ctttcgcgca agcggtgtaa ccaggcgcgc aacagcggcg
    10261 cggctttcat tgcccgggga aagacacggc cagaactgcc gatgaaggtt tccccgccga
    10321 gtgcgtgcgc ccaggcgcgt aactgctccg ggccgaaggc gtcgagcatg gcggcgattt
    10381 cgccgcgccg tgcgccgtag cgtgcgtcga aggccgcacg cggctcggaa tgcgtgagat
    10441 tcagaccacc ccgaccggcc agcaggaatt tgcgcccgac ggagcgcata gcgtcgaaaa
    10501 gttcgaccgc gacgccggcc tggctcaatg tctcggcggc catcaatccc gccggaccgc
    10561 cgccgacgac gaccacgcgc gctttcatgc gccgttgtct tccacacagt cacgcgaatg
    10621 cgcgaaaacg cctcggcgtt ttatggaaat tcgctgcgga ttccggccgt tcggcgaaaa
    10681 ccaccgcttt ttgcggctat agtcgggcgg tgcttgagtc ggcgtggttc gattcatgtt
    10741 tttcgggtgg caagcggtgc ccgggcgcgc attgtgcgcg agaaggcggc cggataaaag
    10801 tccgccccgc atcctgtttt tgatctccat caaatgggcg gcagcgttcc agcttaggct
    10861 cggaccgctg agaaggagtg cgtcctgagt acggcagtgg cggttgccgc gacgagcgac
    10921 atccgcgaaa cccccagttg cttccattgt ggcttgcccg ccgacggcga tggcggccgg
    10981 cgcacgctcg tcggtggcga atggcgcgac ttctgctgtc ccggctgcga ggccgttgcg
    11041 agcaccattc tggatcaggg gctggccgac tattaccgcc tgcgcgaaat ggcgccggcg
    11101 cgccctgagg atgtcgaaga catctctccg gtttacgacg atcccacctt ccaggcttcg
    11161 ttcgtgcgcg tgcatcccga cggcaccagc gaggccagcc tgttgctcga aggcgtgcgt
    11221 tgcgccgctt gcgtatggct gatcgaggaa accctcaagc gcgtcgaagg tgtgcaatcg
    11281 gctgaagtca actatgttac gcgccgtgcg cgcttacgct ggaatcccgc gcagacgccg
    11341 ctcagcggtg tgctgcgtcg cattcgcgcc atcggctacg gcgcgcatcc ctatcagccc
    11401 gatcgcgcag aactgctgcg caaggccgag caacgtcaat ggctgtggcg cctgttcgtc
    11461 gccggcttcg gcatgatgca ggtgatgatg tacgccttcc cggtctatgt ggccggcgac
    11521 ggcaccatga cgcaggacat cgagcagatg atgcgctggg ccagcctggt gctgaccgtg
    11581 ccggtcgtcg gctattcggc gcagccgttc ttccgcggtg cgtggcgcga gttgcgcaca
    11641 cgccgcttcg gtatggattt tccggtggcc ctcggcatcg ccgtgggctt cggcgcgagc
    11701 acctgggcca cgatcaccgc gagcggcgac gtctggttcg acagcgtgac catgttcatc
    11761 gccgtgctgc tgctcggccg ctatctggaa ctgcgcgcac gcgcgcgcgt cagcgatgcg
    11821 ctcagtcatc tggcgcgcct cgtgcccgcc gcaggcgagc ggctgctcga ttacccgcgt
    11881 acgcgcgaag tcgagcgcat tgcggtcgcg catctggccg cgggcgatta cgtgctggtc
    11941 aaacccggcg agccggtgcc cgccgacggc gtggtggtgg aagggcacag cgaagtcaac
    12001 gaaagcatgc tgaccggcga gagccggccg gtgagcaaaa ccgtcggcag cgcgctggtg
    12061 ggcggtgcca tcaacgtggc aagtccgctg gtcatgcgcg tggaacgcgt gggcggcgac
    12121 accatgctgt cgtccatcgt gcgtctggtg gagcgtgcgt ccacggagcg tcaaccgctc
    12181 gcggaactgg ccgaccgcta cgcgcactgg ttcgtcattc tggtgctggc cgttgccgcc
    12241 ggtacggccg tcgcctggag tttcatcgac agcgagcgtg cattgtggat cacggtgtcg
    12301 gtgctggtgg cgacgtgtcc ctgcgcgctg tcgatcgcca caccggtcgc tttgacggcg
    12361 gcaaccggtg aacttgcccg acgcggcttt gtcgcaacac gcggccatgt ggtggagacg
    12421 ctggcgcgtg cgacggacgt ggtgttcgac aagaccggca cgctgaccga aggcgagccg
    12481 cgcgtgctga atgtgcgcct gtacgccggc atgacgcgcg aacgggccgc ggcgatcgcg
    12541 gccgcgctgg aaagcggatc ggaacacccc atcgcgcgcg cgatcgagcg ctacgctgcc
    12601 gatagcacgc aaacgtcgct gacggccgat gcgctggcca acacgccggg cgagggcgtg
    12661 gagggcgaag tcggagggcg gcgttaccgc ctgggtcgcc gtgcttttgc cgctgcgccg
    12721 tccggttggc ttggggcgga ggtggaggcg acgcgacaca ccgaagcctg gctgtgcgat
    12781 gacagcggtc cgcttgcgtg tttcgaactg ggcgaccgac tcaagccgca ggcgaatgag
    12841 ctggtcgaat cgctgcgcgc ggccggcaag cgcgtgcatc tgttgagcgg cgacggtgcg
    12901 ggcgcggtgc acgaagcggc gcgtgcgctc ggaatcgagc atgtgcgcgc ggacgccacg
    12961 ccggaagaga agggcgccta tgtgcaggcg ctgcagcgcg aaggcgctgt ggtcgcgatg
    13021 gtcggcgacg gcatcaacga tgcgccggtg ctggcgcagg ccgacgtttc catcgccatg
    13081 gcgagcggcg caaccctcgc gcagacgcac gccgatgcgg tgttgttgag cggccagccg
    13141 caggatctga tgcgcgcgct gcgtttgacc gagcgcggcc gcgacatcct gcgacagaac
    13201 ctcgcctggg gcttcggtta caacctgctc gtcattcctg ccgcggtggt cgggctggtt
    13261 tcgccctggt tggccgcgct gggcatgtcg gccagttcgc tgttggtggt gtgtaacgca
    13321 ttgaggctgc gccgcgcgcg gccggagtat tgagaatgga cgtgctgtat ctgctgattc
    13381 cgctgtcgct ggtgctcgta ttcctgatcg ccgtggtgtt ctggcgtgcg ctgaaaagcg
    13441 gccagttcga cgatctcgaa gggcccggtt tcaaaatcct gctcgacgac gatcgtcctt
    13501 ccgatacgcc cccgtcgcag tgatatcgcc cccatggatg agcgatattg cctcagcgaa
    13561 tgatggggcc agccttcatt tgacctacgt caattgagaa tttgattcgg gtcataggat
    13621 acgtccgcga aattgcggcg cattgcgtgc gcgcggatga gaacgaattt catagcaagg
    13681 ggaagcgcct atatggacgc agtaaacaca acctataact acaaggtggt gaagcaattc
    13741 gccatcatga cggtcatctg gggcatcgtc ggcatgtttg tcggcgtggt cgtcgcgacc
    13801 cagttcgtct ttccggattt gacccacggc attccgtggt tgtcgttcgg acgactgcgc
    13861 ccgctccata ccaacgccgt cattttcgcc ttcggcggtt gcggcctgtt cgcgacttcc
    13921 tattacgtgg tgcagcgcac ctgtcacgtc cgcctgttca gcgaccggct ggcgtcgttc
    13981 accttctggg gctatcaggc ggtgatcctg ctggcggtca tcacgctgcc gctcggcatc
    14041 acctcgtcga aggaatatgc cgaactcgag tggccgatcg acatcctact cgccatcgtg
    14101 tgggtgtgtt acggcatcgt gttcttcggc accatcgcca agcgcaaggt gccgcatatc
    14161 tatgtggcga actggttctt cggcggcttc atcctcgccg tggccttgct gcacatcgtc
    14221 aacagcatgg cgctgcccgc cacgctgacc aagtcctact ccgcctacgc gggcgttcag
    14281 gacgcgatgg tgcagtggtg gtacggccat aacgcggtgg gcttcttcct gaccgcgggc
    14341 ttcctcggga tgatgtacta cttcgtgccg aagcaggccg agcgtcctgt gtattcctat
    14401 cgcctgtcgg tcgtgcactt ctgggcgctg atcttcacgt acatgtgggc cggcccgcac
    14461 catctgcact acaccgcgct gcccgactgg acgcagagcg tgggcatgat cttctcgctc
    14521 atcctgctcg cgccctcctg gggcggcatg atcaacggca tcatgaccct ctcgggcgcg
    14581 tggcacaaac tgcgcaccga cccgatcctg aagttcctga tcgtttcgct gtccttctac
    14641 ggcatgtcga ccttcgaagg tccgatgatg gccatcaaga cggtgaacgc gctgtcccac
    14701 tacaccgact ggaccatcgg tcacgtgcac gccggtgcgc tgggctgggt gggcttcatc
    14761 accatcggct cgctgtacta cctgattccg cggatgtacg gccgtacgga aatgtggagc
    14821 accaagctga tcacggtcca tttctggatc gcgaccatcg gcgtggtcct gtacatcgcc
    14881 gcgatgtgga tctcgggtgt cacgcagggt ctgatgtggc gcgcggtcaa cgatgacggc
    14941 acgctgacct acaccttcgt cgaatcgacc aaggcgagca tgccgttcta cgccatccgt
    15001 ctgctcggcg gcctgatgta cctcagcggc atgatcatga tgttctacaa cctcctcaag
    15061 acggcggcga tcggccggca ggtggacgcg cgcattcccg cggtcgaagc accggcgcac
    15121 gcctaacgcg aggctttatc gaaaatggca cttacacaag agaaaatcga gcgcaacgtt
    15181 gccttgctga tcatcgtgac cgtactggtc gtgagcgtcg gcgggctggt cgagatcgtt
    15241 ccgctgttct tccagaaatc gaccaccgag ccggtggcgg ggctcaagcc ccccaccgcg
    15301 gtgcaactgg ccgggcgcga catctacatc cgcgaaggct gtaacaactg ccactcgcag
    15361 atgatccgtc cgttccgtgc ggagaccgaa cgctacggcc actattcggt ggccggcgaa
    15421 ttcgtgtacg accgcccgtt cctgtggggt tcgaagcgca cgggacccga cctcgcgcgc
    15481 gtcggcggcc gctacagcga cgactggcac cgcatccacc tgatcaaccc gcgcgacgtc
    15541 gtgcccgagt cgatcatgcc cgcgtatccc tggctggaga acacgccggt ctacagcggc
    15601 gacatcgagg cgcgcatgaa gacactgcgc aagctgggcc tgccgtatac cgacgaggac
    15661 attgccggcg ggaagaagat gctcgagggc aaaaccgaaa tggatgtgct gatcgcttat
    15721 ctgcaaagcc tcggcctcgc cctcaaagat cgcaagtgag acgcaaatgg aatggttgaa
    15781 cgatctgcgt gtgctgatca ccgtactgag tttcctgtcc ttcctcggca tcgtcttctg
    15841 ggcgtaccgg ccgaagagcc aggcgggatt cgaacgcgcc gcacaatcgg tgcttgagga
    15901 agaagagccg ggggggcagc aatgaccgac ttcgtaaacg gattctggga gttctacgtc
    15961 ggcatcatca cccttgccgg cgtgatcggc tgctggctgc tgctgcagtc gcagaacaaa
    16021 gtgaaggcca agaagaacca acaggtcgag accaccggcc acgtgtggga cggcgatctg
    16081 gccgaactga acaacccgct gcccaactgg tggcgctgga tgttctattt gaccatcatc
    16141 ttctcgctcg gctacctggt gctttatccg ggcctcggct ttttcgacgg gacgctcggc
    16201 tggagctcgg ttcagcgcta cgagaacgag cagacgaagg cggaggagca atacggtccg
    16261 ctgtacgcca agtacacgca gatgtccgtc gaggaagtgg ctgccgaccc ggcggccatc
    16321 gcgatggggc agaacctgta cttgaactac tgcgcacagt gccatgcctc ggatgcgcgc
    16381 ggcagcaagg gcttccccaa cctcgccgat cgcgactggc tttggggggg cgatcccaag
    16441 accatcacgc aaaccatcat gcaaggccgt accggcatga tgccgccgat gggtcccgcg
    16501 ctgggcagcg agggcacgcg cgatgtcgcc catttcgtgc gctcgctgtc gggcctcacg
    16561 catgactcga ttcgtgcgac gcgcggccgg ccgctgttcg cgcagaactg cgcggtctgc
    16621 cacggcgcgg acgggacggg caatcagatg ctgggtgccc ccaacctgac cgaccggaca
    16681 tggctgtatg gcagttcgga ggcgacgatc atcgagacga tcacgaaggg gcgcaacaac
    16741 gtcatgcccg cgtggaagga gttcctcggc gaatccaaga ttcacgtcct cgctgcctac
    16801 gtctggagcc tgtcgaacga accggctcag cgtgatccgt tggccgaacc gatgaagacc
    16861 gggccggagc aaaccgaacc ggtcaaagtt ggtcagaact agcaagcctg aagattgcgc
    16921 gggcgagggc ccgcgcaatc cttttgcagc acacaccgaa cgtcctgata cggagcagcc
    16981 tgtgagtcgc cggttggaag acataccggt cgtgcaagtc ggcgcgatcg atgaagtcga
    17041 gcaaagcctt tacgaagtcc gcaagcacat ctacgtgcgc gccgtgcatg gctggtttgc
    17101 acgatggcgc tggattttcg tgtggctgac tcagatcgtc ttctacggtc tcgcctggat
    17161 ggaatggaac ggccgccaag cggtgctgtt cgacctggcc gcgcgcaagt tctacatctt
    17221 cggctatgtg ttctggccgc aggacttcat ctatctcacg gcgctgttga tcatcagcgc
    17281 ctattcgctg ttcctgttca ccgccatcgg cggacgccta tggtgcggct acgcctgtcc
    17341 gcaaacggtc tacaccgaga tcttcatgtg gatcgagcgc aagatcgagg gcgatcgcca
    17401 gcgccgcatg cggctcgatg cgcaaccaat gtcgccgcgt aaattcgcac tgaagacggc
    17461 caagcacggc gcctggatcg ccgtggccct gtggaccggc ttcaccttcg tcggctactt
    17521 cacgccgatc cgcgaactgg gcggcgatat cctcacgctg gacatcggcc cgtgggcgac
    17581 cttctggctg ttgttctacg gctttgccac ctacggcaac gccggctgga tgcgcgagca
    17641 ggtatgcaag tacatgtgtc cttacgcgcg cttccagggc gtgatgttcg acaaggacac
    17701 catggtcgtc acctacgaca cggcgcgcgg cgagccccgc ggtgcgcgcg gacgcaaggc
    17761 agatccgaag gcacagggtc tgggcgactg tatcgactgc acgatgtgcg tgcaggtctg
    17821 tccgaccggc atcgatatcc gcgacggtct gcaatacgag tgcatcggct gcgccgcctg
    17881 tatcgacgcc tgcgatacgg tgatggacaa gatgggctat gcccggggct tgatccgcta
    17941 ctcgaccgag aatgcgatgg cgcagggctg gaacttcggt cagatggtgc gacgagtgtt
    18001 ccgcccgcgc gtgctgatct acagcgcgat tctgctggcg atcatttccg tcaccgttta
    18061 ctcgctctat gcgcgcgtgc cgctcaaggt ggatgtgctg cgcgaccgcg ccagcctcat
    18121 gcgcgaagtc gaggatggcc tgctcgaaaa cgtctatcgg ctgcagatca tgaacacgca
    18181 ggaagtgccg catatgtacc gcattaccgt gacgggcctg gaaagcctgc acgtcgcgag
    18241 cgacgacgtg ttcgaactgc cggctgccgg ttcgcggcag gtgccgctgc gcctccgcat
    18301 ggacggt
//
LOCUS       DATGOU010000035         3332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_1412043, whole genome shotgun sequence.
ACCESSION   DATGOU010000035 DATGOU010000000
VERSION     DATGOU010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412043"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..2408
                     /gene="parC"
                     /locus_tag="VKZ48_01970"
     CDS             111..2408
                     /gene="parC"
                     /locus_tag="VKZ48_01970"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HLU76654.1"
                     /translation="MAGELGDSLPLGRYAALQYLQYAVSTVKDRALPRVGDGQKPVQG
                     RILYSMWDTGTRAGAKRTKSAAVVGDVLGRFHPHGDQSVYDALVRLAQDFTMRYPLID
                     GEGNFGSRDGDEAAAYRYTEARLTKFAEVLLGELDQGTVDFIPNYDGTRKEPTVLPAR
                     LPIVLLNGSSGIAVGMATEIPPHNLNEVANAAVAMIRDPEISVAGLMKYIKGPDFPGG
                     GQIITPRAEIKEAYQSGRGSVRVRARWTTERLARGQWQIVVTELPPGTSARKVLEEID
                     AITNPQPKSGKKSLSAEQQREKQLMLSMLDKARDESDRANPVRLVFEPKTSKINEDDF
                     VNLLLAKTSMETNVSINLVMVGLDGRPTSKTLRDVLFEWLQFRFATVTRRTQHRLGQV
                     LDRIHVLEGRMIVLLNVDKVIKIIRNSDEPKADLIKAFKLSERQAEDILEIRLRQLAK
                     LEHIKIEQELKNLRTEQKGLDKVLGSRKALEDLVVSEIEADAKTFGDKRRTRIEESEK
                     AAVETPVLDEPVTVIFSKNGWVRARQGWNVEASALSFKEGDSLQALIQCRTVDPVVFL
                     DSKGRAYTVEVGQLPPSRGDGAPASSLVDVQDGAKIMYCVAGKPDTHVLVGTTGGYGF
                     YTKIGDMVSNRRAGREFLNVDKGETPIPPFMFEPSPKSMVVALAEEPRVLVYGLDELK
                     YMAKGRGLILMGLEDKEKLVAAVVTSERQVVVSGMLRGKEKEVTLSGAKFNHHVGRRA
                     RMGRVLSDKLKAPLKLSVSTRPSDE"
     gene            2463..2561
                     /gene="ffs"
                     /locus_tag="VKZ48_01975"
     ncRNA           2463..2561
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VKZ48_01975"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2719..>3332
                     /gene="dnaX"
                     /locus_tag="VKZ48_01980"
     CDS             2719..>3332
                     /gene="dnaX"
                     /locus_tag="VKZ48_01980"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HLU76655.1"
                     /translation="MSYQVLARKWRPRSFASLVGQEHVVKALTHALEQQRLHHAYLFT
                     GTRGVGKTTLARILAKSLNCESGITAQPCGTCAACTEIDAGRFVDLLEVDAATNTKVD
                     EMRQLLENAVYAPTRGRFKVYVIDEVHMLSNSAFNSMLKTLEEPPEHVKFILATTDPQ
                     KIPVTVLSRCLQFNLKQMPQAAITSHLQRIMAEEGLQGDADALRL"
BASE COUNT          662 a         1022 c         1107 g          541 t
ORIGIN      
        1 tcgaggaacg gcccgaaatc aaggaaaaac cgacgccgca ggccgtcgag aacaccctcg
       61 tgccaagcgg cggcggcggg ggcggacgcg tgccgccgaa tttcgcgccc atggccggcg
      121 aactgggcga cagcctgcct ctgggccgtt acgccgcact gcagtatctg caatacgccg
      181 tcagtacggt gaaggatcgc gcgctgccgc gcgtgggcga cgggcagaag ccggtgcagg
      241 gccgcattct ctattccatg tgggatacgg ggacgcgcgc cggtgccaag cgcaccaagt
      301 cggcggcggt ggtgggcgat gtgctgggac gcttccatcc gcacggcgac cagtcggtct
      361 atgacgcact ggtgcgcctg gcgcaggact tcaccatgcg ctatcccctc atcgacggcg
      421 aggggaattt cggctcccgc gacggcgacg aggcggcggc gtatcgctat acggaagcgc
      481 gtctgaccaa gttcgcggaa gtgctgctcg gcgaactgga tcagggcacg gtcgatttca
      541 ttcccaacta cgacggcacg cgcaaggagc cgactgtgct gccggcgcga ctgcccatcg
      601 tgctgctgaa cggttcgtcc ggcatcgcgg tcggcatggc gaccgaaatc ccgccgcaca
      661 acctcaacga agtggcgaac gcggccgttg cgatgattcg cgatccggag atctcggtcg
      721 ccggattgat gaagtacatc aaaggcccgg actttcccgg cggcgggcag atcatcaccc
      781 cgcgtgccga aatcaaggag gcgtatcaga gcggccgcgg ctcggtgcgc gtgcgtgcgc
      841 ggtggacgac cgagcggctc gcacgcggcc agtggcagat cgtggtcacg gaactgccgc
      901 cgggcacctc cgcgcgcaag gtgctggagg aaatcgacgc catcaccaac ccgcagccga
      961 aatccggcaa gaagtcgctt agcgccgagc agcagcgcga gaagcagttg atgctgtcca
     1021 tgctcgacaa ggcgcgcgac gaatccgatc gcgccaatcc cgtgcggctg gtgttcgagc
     1081 cgaagacttc gaaaatcaac gaagacgact tcgtcaatct cctgctcgcc aagacgagca
     1141 tggagacgaa cgtttcgatc aacctcgtga tggtcggcct ggacggccgg ccgacgagca
     1201 agacgctgcg cgacgtgctg ttcgaatggc tgcagttccg cttcgccacg gtgacccggc
     1261 gcacgcagca tcgtctgggc caggtgctgg accgcatcca cgtgctcgaa gggcgcatga
     1321 tcgtcctgct caacgtggac aaggtgatca agatcatccg caactcggac gagcccaagg
     1381 ccgatctgat caaggcgttc aagctctccg agcgtcaggc cgaagacatt ctcgaaatcc
     1441 gcttgcgcca gttggccaag ctcgaacaca tcaagatcga gcaggaactg aagaatttgc
     1501 gtaccgagca gaaagggctg gacaaggtgc tcggctcccg caaggccctg gaggatctgg
     1561 tggtgtccga aatcgaggcg gatgccaaga ccttcggcga caagcgccgc acccgaatcg
     1621 aggaatcgga gaaggccgcc gtcgagacgc cggtgctgga cgaaccggtg accgtcatct
     1681 tctcgaagaa cggctgggtg cgcgcccggc agggatggaa cgtggaagcc tctgcgctgt
     1741 cgttcaagga aggcgacagc ctgcaggcgc tcatacagtg ccgcacggtc gatccggtgg
     1801 tgttcctgga ttccaaaggg cgcgcgtaca cggtggaagt cggtcagttg ccgccttcgc
     1861 gcggcgacgg cgcgccggcc tcctcgctgg tcgatgtgca ggacggcgcc aagatcatgt
     1921 attgcgtggc cggcaagccg gatacccatg tgctggtcgg cacgacgggc ggttacggct
     1981 tctacacgaa aatcggcgat atggtttcca atcgccgggc gggccgcgaa ttcctcaatg
     2041 tggacaaggg cgagacgccc attccgccgt tcatgttcga gccgtcgccg aagtcgatgg
     2101 tggtggcgtt ggccgaagag ccgcgcgtgc tcgtctacgg actggacgaa ctcaaataca
     2161 tggccaaagg gcgcgggctg atcctcatgg ggctggagga caaggaaaag ctcgttgccg
     2221 ccgttgtgac ctcggagcgg caggtcgtcg tgagcgggat gctgcgcggc aaggagaagg
     2281 aagtgacgct ctccggcgcg aagttcaacc accatgtcgg ccgtcgcgcg cgcatgggtc
     2341 gggtgttgtc agacaagctg aaggcgccgt tgaaactgag cgtgtcgacc cggccgtccg
     2401 acgaatagcg catgctaaac tgcgcggaca tttgcaaacg cacgacagtg cggcagatcg
     2461 acggcgggcc tccccgcatt gcagcgtagt gaacctggtc aggtccggaa ggaagcagcc
     2521 acagctactc cctgcaagtg ccggggttcg ggctcgccac ccttatttct gcgcgtgccg
     2581 ggtcggggag cgcccgattc ggcaccgaag atgagcgcga gtgcgcgaag ctgtacgagc
     2641 gggggcaaag caacgctcag gcggcacgaa ccggcaacgg cgttgatcga acaaccgtaa
     2701 tcggagcctt ctcagacgat gtcctatcaa gttctcgccc ggaaatggcg gccgcgcagt
     2761 ttcgcctcgc tggtcgggca ggagcacgtc gtcaaggcac tgacgcatgc gctcgaacag
     2821 cagcggctgc atcacgccta tctgttcacc ggcacgcgcg gcgtgggcaa gaccacgctc
     2881 gcgcgcatcc tcgccaaatc cctgaactgc gaatcgggca tcaccgcgca gccctgcggc
     2941 acctgtgccg cctgcacgga aatcgatgcc gggcgcttcg tcgatctgct ggaagtcgac
     3001 gcggccacca acaccaaggt ggacgagatg cgccagttgc tcgagaacgc ggtgtatgcg
     3061 ccgacgcgcg gccggttcaa ggtttacgtc attgacgaag tacacatgct gtccaattcc
     3121 gccttcaact cgatgttgaa gacgctggaa gagccgccgg agcatgtgaa attcattctc
     3181 gccaccaccg acccgcagaa gattcccgtg acggtgctgt cgcgctgtct gcagttcaac
     3241 ctcaagcaga tgccgcaggc ggcgatcacg tcgcatctgc agcgcatcat ggccgaggag
     3301 ggcttacagg gcgatgcgga cgcgctgcgt ct
//
LOCUS       DATGOU010000036         6957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_4079924, whole genome shotgun sequence.
ACCESSION   DATGOU010000036 DATGOU010000000
VERSION     DATGOU010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4079924"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /locus_tag="VKZ48_01985"
     CDS             <1..177
                     /locus_tag="VKZ48_01985"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005433680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HLU76656.1"
                     /translation="TTGEPMDKAGAYGIQGRAAMFIEDIRGSYTGIMGLPLYETSELL
                     AAFGYPLWSQSATP"
     gene            263..1717
                     /gene="rng"
                     /locus_tag="VKZ48_01990"
     CDS             263..1717
                     /gene="rng"
                     /locus_tag="VKZ48_01990"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HLU76657.1"
                     /translation="MTVEFLINFTPQETRVALIEQGVVQELHVERTASRGIVGNVYLG
                     KVVRVLPGMQSAFIDIGLARTAFLHVADIWSDRQNGEGARPIERILSEGQSLVVQVLK
                     DPIGTKGARLSTQISIAGRLLVHLPQDSHIGISQRIEDETERRTLRERLQRIIPPDVK
                     GGFIIRTMAETATDEELANDIAYLRRLWDNIRERAATAAVPALLYHDLWLAQRVLRDF
                     VNADTGRIVVDSRENYLRLESFARTYMPNVAHKLEYYTGERPLFDLYNIEEEIQRALE
                     RRVDLKSGGYLILDQTEAMTTVDVNTGGFVGVRNFDDTVFKTNLEAAQVIARQLRLRN
                     LGGIIIIDFIDMDNPDHREAVLAEFTKALSRDHTRMTVNGFTALGLVEMTRKRTRESL
                     EHVLCEPCPTCGGRSVVKTARTVCYEILRELLREARQFNAREFRILAAQSVIDLFLEE
                     ESQSLAMLGDFIGKPVSLQVETSYTQEQYDIILI"
     gene            1793..2128
                     /locus_tag="VKZ48_01995"
     CDS             1793..2128
                     /locus_tag="VKZ48_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76658.1"
                     /translation="MAQLSDKEKALIDQAQRDAGAGASTLRTDWDDSLLGAESAFPET
                     VIGWDNPAADAPGGDRSSDKWAYIATMIEAERAEAQAARARTRRIVLSVTGVVVLILL
                     AAGLQAFLG"
     gene            2216..3100
                     /locus_tag="VKZ48_02000"
     CDS             2216..3100
                     /locus_tag="VKZ48_02000"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HLU76659.1"
                     /translation="MNRPVAFDDSDFDSLLPAVPFTRRGFLVSSLATGFALATHPVMA
                     QSVITTPSTGLEAGETKIPVSDGELPAYYAAPEKAGRYPVILVVQEIFGVHEHIKDVC
                     RRFAKLGYLAVAPELYARQGDVSGLTDIAAIREVVAKVPDAQVMSDLDAAATWAGKHA
                     KGHAAKLGITGFCWGGRIVWLYAAHNPDLDAGVAWYGRVAGDATPNTPRHPLDVAADL
                     KAPVLGLYGGADQGIPVSTVERMRAALKAADKKSEIVLYPDAPHAFHADYRPSYRKEV
                     AEDAWKRCVEWFRTHGLG"
     gene            complement(3157..4137)
                     /locus_tag="VKZ48_02005"
     CDS             complement(3157..4137)
                     /locus_tag="VKZ48_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein BugD"
                     /protein_id="HLU76660.1"
                     /translation="MRACKAVVGAIALGLGVFSAGVGAQNFPTKPVTMIVPFSAGGPT
                     DTVARSIAAAMQESLKQTVVVENVGGAGGTIGVARGARSAPDGYTITMHHIGMATAPT
                     LYRKLSFDPLKDFEYIGLVADVPMTMIARADFPPKDVKEFLSYVKANKDKLAYANAGL
                     GAASHLCGLMFMTAIDTAFTTIPYKGTGPAMNDLLGGQVDFMCDQTTNTTSQIKAGKV
                     KAYAVTTPERVAALPDLPTMQEAGLKGFEVTVWHGLYAPKGTPKPVMDTLVKALQHAL
                     QDETVKSRFADLGTAPVPVERGTPEALRKHLAAEIQKWRPVIQAAGQYAD"
     gene            complement(4401..4477)
                     /locus_tag="VKZ48_02010"
     tRNA            complement(4401..4477)
                     /locus_tag="VKZ48_02010"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4441..4443),aa:Arg,seq:ccg)
     gene            4646..5179
                     /locus_tag="VKZ48_02015"
     CDS             4646..5179
                     /locus_tag="VKZ48_02015"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLU76661.1"
                     /translation="MKDEGHDHAHSSPIKTPKQLVVVIVLSFLIPILGIILLTQFVLS
                     GKAPSPAALDTEAIAERIKPVADVVLSGSAEATAEQTAAAAAPAASGAQAAAGPVSGE
                     AVFNQACAACHGTGVMGAPKIGDAAQWGGRISKGKDGLYASALNGMGLMPPRGGNLAL
                     TDEQVKAAVDYILESSK"
     gene            5352..6632
                     /gene="gshA"
                     /locus_tag="VKZ48_02020"
     CDS             5352..6632
                     /gene="gshA"
                     /locus_tag="VKZ48_02020"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HLU76662.1"
                     /translation="MLPHLTTALSGPLANLEARILDASASIEHWLRGKWLEHTPPIYG
                     SVDLRNSGFKLAPVDMNLYPGGFNNLNPQFMPLCVQAAMTAVEKICPEAHNLLLIPEN
                     HTRNTFYLQNVVRLQTIFRQAGLNVRLGSLLPDITEPTEIEVPDHPPLVLEPLVRHGN
                     RLGLADFDPCAILLNNDLSAGVPDILRGLDEQFVLPPLHAGWATRRKSNHFAAYDRVA
                     AEFAELIDIDPWLVNPYFTRCGEVNFQARSGEECLTANVTALLERMRAKYREYGIQET
                     PYVVVKADAGTYGMGIMTARSPEEVIGLNRKQRNKMAVVKEGLAVHEVLIQEGVPTFE
                     TINDAVAEPVVYMIDRYVVGGFYRVHTGRGKDENLNAPGMHFEPLAFDDCCTQPDPFA
                     AADAAPNRFYAYGVIARLALLAAAIELEETAPAE"
     gene            6629..>6957
                     /locus_tag="VKZ48_02025"
     CDS             6629..>6957
                     /locus_tag="VKZ48_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HLU76663.1"
                     /translation="MKLCFILDPLPLIKSAKDSTYAMMVEAARRGHAVEVMMQEALMW
                     KGGRVLGRTTPITLTGDEADWYRLDQPRETALTGFDAVLMRKDPPFDMEYVTSTWLLE
                     RAVAEGA"
BASE COUNT         1259 a         2324 c         2184 g         1190 t
ORIGIN      
        1 accaccggcg agccgatgga caaggccggc gcctacggca tccagggccg cgcggcgatg
       61 ttcatcgagg acatccgcgg cagctacacc ggcatcatgg gcctgccgct gtacgagacc
      121 agcgaactgc tcgccgcctt cggctatcct ctgtggtcgc agagcgcgac gccctgaggg
      181 ggcgcgcgcc tttcccccga tgagccatgc gcaatggcgg cgttccgctc cgtcattgcg
      241 catttcccat taggccaggc caatgaccgt cgaattcctc atcaacttca ccccccagga
      301 aacccgcgtc gccctcatcg agcagggcgt ggtgcaggag ctgcacgtcg agcgcaccgc
      361 cagccgcggc atcgtcggca acgtctacct cggcaaggtg gtgcgcgtgc tgcccggcat
      421 gcagtcggcc ttcatcgaca tcgggctggc gcgcaccgcc ttcctccatg tcgccgacat
      481 ctggagcgac cgccagaatg gcgagggcgc gcgcccgatc gagcgcatcc tgtccgaagg
      541 ccagagcctg gtggtgcagg tgctcaagga cccgatcggc accaagggcg cgcgcctgtc
      601 cacccagatc agcatcgccg gccgtctgct ggtgcatctg ccgcaggact cgcacatcgg
      661 catttcccag cgcatcgagg acgaaaccga acggcgcacg ctgcgcgaac ggctgcaacg
      721 gatcatcccc cccgacgtca agggcggctt catcatccgc accatggccg agacggcgac
      781 cgacgaggag ctggccaacg acatcgccta cctgcgccgc ctgtgggaca acatccgcga
      841 acgcgccgcc acggcggcgg tgccggcgct gctctatcac gacctgtggc tggcccagcg
      901 ggtattgcgc gatttcgtca acgccgacac cggccgcatc gtggtcgact cgcgcgaaaa
      961 ttatctccgg ctggagagct tcgcccgcac ctatatgccc aatgttgcgc acaagctgga
     1021 gtactacacc ggcgagcggc cgctgttcga tctttacaac atcgaggagg agatccagcg
     1081 cgcgctcgag cggcgggtcg atctcaagtc gggcggctat ctgatcctgg atcagaccga
     1141 ggccatgacc accgtggacg tgaacaccgg cggcttcgtc ggcgtgcgca atttcgacga
     1201 cacggtattc aagaccaatc tggaggcggc gcaggtcatc gcgcggcaac tgcgactgcg
     1261 caacctcggc ggcatcatca tcatcgactt catcgatatg gacaaccccg accaccgcga
     1321 agcggtgctg gcggagttca ccaaggcgct gtcgcgcgat cacacccgga tgaccgtcaa
     1381 cggcttcacc gccctcgggc tggtggaaat gacacgcaag cgcacgcgcg aaagcctgga
     1441 gcacgttctg tgcgagccct gccccacctg cggcggtcgc agtgtggtga aaaccgcgcg
     1501 caccgtctgc tacgaaattc tgcgcgaact gctgcgcgag gcacgtcagt tcaacgcgcg
     1561 ggaattccgc atcctggcgg cgcaatcggt catcgatctg ttcctcgagg aggagtcgca
     1621 gtccctcgcc atgctggggg atttcatcgg caaaccggtg tcgctgcagg tggagacgag
     1681 ctatacgcag gagcagtacg acatcatcct gatctaggac gcggaaagcg ccaccaaagc
     1741 gatgctgatc gggcgttcgc cgctcaattt ttgcgctagt cttggaatcg ccatggccca
     1801 gctttccgac aaagaaaaag ctctgatcga ccaggcgcag cgcgacgccg gtgccggcgc
     1861 gtccacgctg cggaccgatt gggacgactc gctgctgggt gccgagtcgg cttttcccga
     1921 aaccgtcatc ggctgggaca acccggcagc cgacgcgccc ggcggagacc ggtccagcga
     1981 caagtgggcc tacatcgcca ccatgatcga agccgagcgc gccgaggcgc aggccgcccg
     2041 tgcacgcacc cggcgcatcg tccttagcgt caccggcgtg gtggtgttga tcctgctggc
     2101 ggccggcctg caggccttcc tcggctagcc gccgcacaaa gagcgctacc gatccgccag
     2161 gaacacgcgg cacaatgcgt gctccccgtt tggcatccct gcccaggagt ccgtcatgaa
     2221 ccgtcccgtg gctttcgacg acagcgattt cgacagcctg ctgcccgcgg tgccgtttac
     2281 gcggcgcggc tttctggttt ccagcctggc gaccggcttc gccctcgcca cccatcccgt
     2341 catggcgcaa tcggtcatca ccacgcccag caccggactg gaggcgggcg agaccaagat
     2401 tcccgtgagc gacggcgagt tgccggcgta ttacgccgca ccggagaaag cggggcgcta
     2461 tcccgtgatt ctggtggtgc aggaaatctt cggcgtgcac gaacacatca aggacgtctg
     2521 ccggcgcttc gccaagctgg gctatctcgc cgtggcgccc gagttgtatg cccgccaggg
     2581 cgatgtcagc ggtctgaccg atatcgccgc aatccgcgag gtggtcgcca aggtgccgga
     2641 tgcgcaagtc atgagcgatc tggatgcggc ggcaacgtgg gctggcaagc acgccaaagg
     2701 ccatgccgcc aagctcggta tcaccggttt ctgctggggc ggccggatcg tgtggctcta
     2761 tgccgcgcac aacccggatc tcgatgccgg cgtggcctgg tacggccgcg tggccggcga
     2821 cgccacgccg aacacgccgc gtcatccgct ggacgtcgct gccgatttga aggcgccggt
     2881 gctgggtcta tacggcggcg ccgatcaggg cattcccgtt tccaccgtcg aacggatgcg
     2941 cgcggccctc aaggccgccg acaagaaaag cgagatcgtg ctatatccgg acgcgcccca
     3001 tgccttccac gccgactacc ggccgagcta ccgcaaggag gtggcggaag acgcctggaa
     3061 gcgctgcgtg gagtggttcc ggactcacgg cctgggttga tacggaaata aaaagggcgg
     3121 cccgcaggcc gccctttctt gtcgccggat cgtcgctcag tcggcgtatt gaccggcggc
     3181 ctggatgacc ggacgccatt tctggatttc cgccgccagg tgcttgcgca gcgcttcggg
     3241 cgtgccgcgc tcgaccggca ccggtgcggt gcccagatcg gcaaagcgcg acttcaccgt
     3301 ctcatcctgc agcgcatgct gcagcgcctt caccagcgta tccatcaccg gcttgggcgt
     3361 gcccttgggc gcatacagtc cgtgccagac cgtgacttcg aagcctttca gaccggcttc
     3421 ctgcatggtc ggcaggtcgg gcagggcggc aacgcgctcg ggtgtggtca ccgcataggc
     3481 tttcaccttg ccggccttga tctgcgaagt ggtgttggtg gtctggtcgc acatgaagtc
     3541 cacctggccg cccagcagat cgttcatggc cgggcccgtt cccttgtagg gaatggtggt
     3601 gaaggccgtg tcgatcgccg tcatgaacat caggccgcaa agatgcgagg cggcgccgag
     3661 gccggcgttg gcgtacgcga gtttgtcctt gttggccttc acatagctga ggaactcctt
     3721 gacgtccttc ggcggaaagt cggcgcgcgc gatcatggtc atcggcacgt cggccaccag
     3781 tccgatgtat tcgaagtcct tcagcggatc gaagctcagc ttgcggtata gcgtaggcgc
     3841 cgtcgccatg ccgatgtgat gcatggtgat cgtgtagccg tcgggcgcgg aacgcgcgcc
     3901 gcgcgccacg ccgatggtgc cgcctgcgcc gccgacgttc tccaccacta ccgtctgctt
     3961 cagcgattcc tgcatggccg cggcgatgga gcgcgccacc gtatcggtgg gtccaccggc
     4021 ggagaagggg acgatcatgg tgaccggctt ggtcgggaaa ttctgcgcgc cgacgccggc
     4081 cgagaacact cccagcccga gggcaatcgc ccccactact gctttacatg ctcgcatcga
     4141 gtcctcctcg ctgtttgaat aagtgcggtc cgtgacaacg cctctgccgc gtccattccg
     4201 gattccacgc gtcggatagt actccagtcg tgccgcgcga taagccatcc gggcaaaggg
     4261 tttcggttcc gattgagaca acaatcgcac ggcgttgtcg aggcttcgac caggcgacgg
     4321 cgaaaatgcg gggaatacag cgactacggc ggcccaccag gaaagacggc gatatggaag
     4381 gggcggcagc cgggagagaa tggcgcgccc gacaggattc gaacctgtga cctttggctt
     4441 cggaaaccaa cactctatcc aactgagcta cgggcgcgcg gcgcggcgtg ccgcgggatt
     4501 gtagcgcaat gctccgggcc gcggctttgc cggcttgagg cttgtcgcta taattcaccg
     4561 attccgttct gcggaaccgc ctgtcgatga cgggcgcgcc attcgaagaa caatttttct
     4621 cctttccgcc aaaggttgcg tacccatgaa ggacgaaggc cacgatcacg cccactcatc
     4681 cccgatcaag acgccgaagc agttggtcgt cgtcattgtg ctgtcctttc tcatcccgat
     4741 cctcggcatc atcctgctca cccagttcgt cctgagcggc aaggcgccca gtcctgcggc
     4801 gctggacacc gaagcgatcg ctgaacgcat caagccggtg gccgacgtgg tgctttccgg
     4861 tagcgccgag gccaccgcgg aacagactgc cgccgccgca gcgcccgccg caagcggtgc
     4921 gcaagccgcc gccggtccgg tctcgggcga agcggtcttc aaccaggcct gtgcggcctg
     4981 ccacggcacc ggcgtgatgg gcgcgcccaa gatcggcgac gccgctcaat ggggcggccg
     5041 catcagcaag ggcaaggacg gtctgtacgc ttcggccctc aacggcatgg gtttgatgcc
     5101 cccgcggggc ggtaatctgg cgctgacgga cgagcaggtc aaggccgcgg tggactacat
     5161 cctcgagtcc tcgaagtaat tccgggaacg caatcaccgt cgaacggggc agcctaggct
     5221 gccccgtttg ccatccgccc cggcgacccc atgggacgcg gcttgcattg ccacaaagtc
     5281 agcgcgggcc gactgggcta gaatcgttcc gatccagaca gccgcccgtt tttctacgca
     5341 atttccatag catgcttcca catctcacca cggcgctgtc cggaccgctg gccaatctcg
     5401 aagcccgcat ccttgatgcc agcgccagca tcgaacactg gctgcgcggc aaatggctgg
     5461 aacatacgcc gccgatctac ggctcggtcg atctgcgcaa cagcggcttc aaactggcgc
     5521 cggtggatat gaacctgtac cccggcggct tcaataacct caatccgcaa ttcatgccgc
     5581 tatgcgtcca ggcggcgatg acggcggtcg aaaaaatctg cccggaagcg cacaacctgc
     5641 tgctcatccc ggagaaccac acgcgcaata ctttttattt gcagaacgtc gtgcgtctgc
     5701 agaccatttt ccgccaggcg ggtttgaacg tgcgcctggg cagtctgctg ccggacatca
     5761 ccgagccgac cgaaatcgaa gtgcccgacc atccgccgct ggtgctcgaa ccgctggtgc
     5821 ggcacggcaa ccgcctcgga ctggccgatt tcgatccctg cgcgattctg ctcaacaacg
     5881 acctgtcggc cggggtgccg gacatcctcc gcggcctcga cgaacagttc gtgctgccgc
     5941 cgctgcatgc gggctgggcg acgcggcgca aatcgaacca cttcgccgcc tacgaccgcg
     6001 tggcggccga gttcgccgaa ctcatcgaca tcgatccctg gctggtcaat ccctacttca
     6061 cccgctgcgg ggaagtgaat ttccaggcgc gcagcggcga ggagtgcctg accgccaatg
     6121 tcaccgccct gctcgaacgc atgcgcgcga aataccggga gtacggcatc caggaaacgc
     6181 cctacgtggt cgtgaaagcc gatgccggca cctacggcat gggcatcatg accgcgcgca
     6241 gtccggagga ggtcatcggc ctcaaccgca agcagcgcaa caagatggcc gtcgtcaagg
     6301 aaggcctggc cgtgcacgag gtgctgatcc aggaaggcgt gccgactttc gaaaccatca
     6361 acgacgcggt cgccgaaccg gtggtctata tgatcgaccg ctatgtggtc ggcggcttct
     6421 atcgcgtcca taccggccgc gggaaggacg agaacctcaa cgcgccgggc atgcatttcg
     6481 aaccgctggc gttcgacgac tgctgcacgc agcccgatcc cttcgctgcg gcggacgcag
     6541 ctcccaaccg cttctacgcc tacggcgtca ttgcgcgact ggcactcctc gccgcggcga
     6601 tcgaactgga agaaacggcg cccgccgaat gaaactctgc ttcattctcg acccgctgcc
     6661 gctcatcaag tcggccaagg attcgaccta cgcgatgatg gtcgaagccg cgcggcgcgg
     6721 ccacgccgtc gaagtgatga tgcaggaagc gctgatgtgg aagggcggcc gggtactcgg
     6781 ccgcacaacg cccatcacgc tcactggcga cgaggccgac tggtatcgcc tcgatcagcc
     6841 gcgcgagacg gcgctgaccg gattcgatgc ggtgctgatg cgcaaggatc cgcccttcga
     6901 catggagtac gtcaccagca cctggctgct ggaacgtgcc gtcgccgaag gcgcaat
//
LOCUS       DATGOU010000037        22105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_2302152, whole genome shotgun sequence.
ACCESSION   DATGOU010000037 DATGOU010000000
VERSION     DATGOU010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 22105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22105
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2302152"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..824)
                     /locus_tag="VKZ48_02030"
     CDS             complement(36..824)
                     /locus_tag="VKZ48_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HLU76664.1"
                     /translation="MSVKRIQSRDNPLFKSLLKLSNSAKERRARRQALLDGPHLIEAY
                     GASGGRVESLIASESGLQRAEIARLFETTPAVSKVVLTDRLFQNIAAVVTPSGIVALI
                     ATPVHEQGPPSDETCLLLEGIQDAGNVGSVLRTAAAAGVRNVLLSKGCAFVWSAKVLR
                     AGMGAHFGLKLYESAPLVEFAQAFEGEVIVTDLQAERSLYELDLRGPVTWVFGSEGAG
                     VTPDLAACATTRIRIPMPGGTESLNVAAAAAVCLYEQVRQTLNA"
     gene            complement(828..1430)
                     /gene="rnhB"
                     /locus_tag="VKZ48_02035"
     CDS             complement(828..1430)
                     /gene="rnhB"
                     /locus_tag="VKZ48_02035"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005667531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HLU76665.1"
                     /translation="MSTARPLVCGVDEAGRGPLAGPVYAAAVILDPARRIRGIDDSKQ
                     LSARDRESLAARIKVRALTWSVAFATVEEIDRLNIYWATMLAMQRAVQALGVAPAEVL
                     VDGNRCPKLTYPVRAIVGGDAKVRCIGAASILAKTARDAEMQQWHERYPHYGFAQHKG
                     YATPEHLESLRRHGPCELHRRSFAPIRLMTDGEPEQVELF"
     gene            complement(1427..2599)
                     /gene="lpxB"
                     /locus_tag="VKZ48_02040"
     CDS             complement(1427..2599)
                     /gene="lpxB"
                     /locus_tag="VKZ48_02040"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HLU76666.1"
                     /translation="MNGAPVRIAMVAGEASGDLLGSRVIAALKARYPHAEFYGIGGPK
                     MIGKGFDSWFPQERLAVHGLVEVLKHLRGLMAIRRELVRRIAASRPHLFLGIDSPDFN
                     LGLAKRLRKRGVTTAHLVSPTVWAWRKGRIKTIREAVSHMLVLLPFEEAIYQDAGVPV
                     SFVGHPLADEIPDTIDRGALLEYLRLPAKSTVITLLPGSRQQEVMNHAEPFIGAAKLI
                     RQRLGNVQFLVPCATRETYELFEQALYQHGEHDLPVRMMFGHAQEALAASDLAIAASG
                     TVTLEAALLRCPMIIAYRMSPMSFAIAKRIIRVKHVGLPNILCDDRVVPEFLQDDVTP
                     ENLAQAALNLYEDKRVLLAIRERFAAVHAVMKRGAAERSAEALAPLIERSLAGGAA"
     gene            complement(2607..3377)
                     /gene="lpxA"
                     /locus_tag="VKZ48_02045"
     CDS             complement(2607..3377)
                     /gene="lpxA"
                     /locus_tag="VKZ48_02045"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HLU76667.1"
                     /translation="MIHPTAIVHPGAKLGANVSIGPYSIVGEYVEIGDNTWIGSHVVL
                     DGHMRIGRDNRIFPFCSLGGEPQDKKYAGEPTRVEIGDRNTIREYCTINAGTVQDAGV
                     TRLGDDNWIMGYVHIAHDCQIGNNTIFASNAQVAGHCHIGDWAILGGMTDVHQFVKIG
                     AHAITGGGTILFQDLPPYVMASGNPAKPFGMNTEGLRRRGFSNEGITALRRAYKTLYR
                     NNLTLAEAIETLAQQQEERPELEILVEFLRQAKRGILR"
     gene            complement(3424..3867)
                     /gene="fabZ"
                     /locus_tag="VKZ48_02050"
     CDS             complement(3424..3867)
                     /gene="fabZ"
                     /locus_tag="VKZ48_02050"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HLU76668.1"
                     /translation="MSTMDINEIQQYLPHRYPFLLVDRVLECVPGERIVAIKNVTFNE
                     PFFPGHFPKLPIMPGVLIIEAMAQAAAILAFKTANRLPSDDSLVYFLGIDEARFKSPV
                     TPGDQLKFTARIERTIKNVWKFAAEATVDGKVAAEAKMMCTMREG"
     gene            complement(3864..4904)
                     /gene="lpxD"
                     /locus_tag="VKZ48_02055"
     CDS             complement(3864..4904)
                     /gene="lpxD"
                     /locus_tag="VKZ48_02055"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HLU76669.1"
                     /translation="MQARTVRLADLVARLGGEVIGDAETRISQVATLRSAGPDHISFF
                     TQGKYRDELLATQAGAVIVSAAERDATTRPRIVCDDPYLYFARVSLLLNPEPAIEPGV
                     HPTAIVDASASIDPSAAVGAGCVIGADAVVGPDVALDAHCVVGPRCTIGQGSRLHARV
                     TLYDDCVIGRRVIIHSGVVLGADGFGYAQDAGRWLKIPQIGRVVIGDDVEIGANTTID
                     RGALDDTVIEEGVKLDNQIQIGHNCRIGAHTAVAGCAGIAGSTTIGKHCLIGGAAMIG
                     GHLHIADRVIVSGGTWVTRSIEEPGMYLSVIPATPGREWRRTVALLRGLDKLNDRVRR
                     IERGMSGTENKE"
     gene            complement(4913..5428)
                     /locus_tag="VKZ48_02060"
     CDS             complement(4913..5428)
                     /locus_tag="VKZ48_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HLU76670.1"
                     /translation="MTLMKFRWLALLPLMALAVPTFAQEGKIGFVNLDRILRDAVPAQ
                     RAQKRIEAEFTKRDQELQRMAEEVKKLQEALERNAMTMQESERSRRERELAEASREFQ
                     RKQREFREDLNQRRNEELAGVLERANRAVKQIAESEKFDIIFQEAVYASPRIDITDKV
                     IKSLDDGKGAK"
     gene            complement(5457..7739)
                     /gene="bamA"
                     /locus_tag="VKZ48_02065"
     CDS             complement(5457..7739)
                     /gene="bamA"
                     /locus_tag="VKZ48_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HLU76671.1"
                     /translation="MKKKLAAALIGALFAHAAHAFDPFVVRDIRVEGIQRIEAGTVFS
                     YLPIRVGETMTQDKATEAIRALFGTGFFKDVRIEVEGNILVVAVEERPAIAQIDFSGM
                     KEFPAEDVKLALRENNVAEGRIFDKATIDKAEQEIKRQYLTRGRYGVTIQTTVTPLDR
                     NRVAINFAVDEGDVAKIARINIVGNQTYTEETLLDLFVLRTPGWFTWYTKNDQYSRQK
                     LSADLETLRSFYLDRGYLDFNIESTQVSITPDKRDIYITVNIVEGEKYTVTDVTLSGN
                     TIVPEEELRKLIQIKPGEAFSRQKLTDSTKAITDRLGNEGYAFANANAVPDVDKEKRT
                     VAFNIMVDPGRRVYVRRIDVVGNTKTRDEVVRRELRQLEGAYYDGEKLQLSRQRIDRL
                     DFFSEVAVDTQPVAGTTDQVDVTYSVTEKPTGALMFGAGFSSADGLVLQGSVSQQNLF
                     GTGNALQIMMNTSKINRNIGLSFTNPYLTVDGVSGGFDLYSRRYDPRRRLSFGRYATD
                     TLGAGLRVGVPVTEFDRINFGLAIEQTEIELFDDSPDRFKDFVDSQGDKTLGLLGTVG
                     WTRDRRDSAFWTTTGTYQRAYLETGLPGVDLKYYKLNYELSWYVPLSRDLTLRLKGEV
                     GYADGYGGGELPFFKAFFGGGPNSVRGFEQGSLGPRDRDGVLGGSRKAVGNAELLFPM
                     PGMQQDRSVRMGVFFDAGQVWDTGSGNTLSDLKLRYSTGGFVSWNSPFGPLQLILAMP
                     LNDEDGDDTQKFQFMFGQAF"
     gene            complement(7759..9123)
                     /gene="rseP"
                     /locus_tag="VKZ48_02070"
     CDS             complement(7759..9123)
                     /gene="rseP"
                     /locus_tag="VKZ48_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HLU76672.1"
                     /translation="MTLLYTVLSFILALGLLVVVHEFGHYLVARWCGVKVLRFSVGFG
                     TPFYIRRLGRDQTEWALAPIPLGGYVKMLDEREGEVPAADLPRAFNRQNVWKRIAIVV
                     AGPAANFLLAIALYATLYVGGVQELKPIAGTPSAHSLAEAAGVRDGDLIRTVNGVPVR
                     SWQDVRWLLLEHAIDRAPGTLEVLTAGRSLERRVLDLSTVTADQSGGDLLMQIGLRPH
                     RPEIEAVVGQVAPGSAAERAGLQQDDHIVAIDGEPVAHWEMLVERVRAAPARQLTLTV
                     MRGDLRLQLDATPEAVGEGDARYGRLGVGPRIDEARMQEYFVDIRYGLGEALVRATQK
                     TWDVSIFSLRMLGKMVMGEVSWRNLSGPVTIADYAGQSAQLGLAPYLAFLALISVSLG
                     VLNLLPIPMLDGGHLMYYTAEILKGSPVSERIMEIGQRAGLAVLLALMVFAFYNDISR
                     LLTG"
     gene            complement(9120..10322)
                     /gene="ispC"
                     /locus_tag="VKZ48_02075"
     CDS             complement(9120..10322)
                     /gene="ispC"
                     /locus_tag="VKZ48_02075"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HLU76673.1"
                     /translation="MTVVQRIAVLGATGSIGRSTLDVVARHPDRFRIHALTAHRNIDA
                     LYEACRVHRPAVAVVADAAGARALSARFEADGIDIAVLSGEQGLVEAVTHPDVDSVMA
                     AIVGAAGLKPTIAAASAGKKILLANKEALVMAGPIFMAAVRAGGATLLPIDSEHNAIF
                     QCLPALASGRLTDCGVRRILLTGSGGPFRTRPLAELTQITPDQACAHPVWSMGRKISV
                     DSATMMNKALEVVEAHWLFGAPAQQIEVVIHPQSVVHSMVEYADGSVIAQLGHPDMRT
                     PIAQALAYPERVDAGVGFLDLAKIGTLNFEQPDFQRFRALALAYDVLTAGGSAPAVFN
                     AANEVAVEAFLNAELSFPRITEVIEATLERLPAQRIESLEDVTEADRRARLEASALVR
                     GRSLAKTA"
     gene            complement(10319..11149)
                     /locus_tag="VKZ48_02080"
     CDS             complement(10319..11149)
                     /locus_tag="VKZ48_02080"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HLU76674.1"
                     /translation="MLRDRVITALILIGVLLGGLFFLPALGWALFIGIALYCAAWEWA
                     GLAGYASAQRVVYGAVLTAVGLGWVYATGILSGAISYGAVSMAYLLAAAFWAICMPFW
                     LRSPAHKLPGMLLLLAGVVVLLPCYAALVQLREVHPVSLLLFMATVWIADIAAYFTGR
                     RFGRRKLAPSISPGKTWAGVYGAFVATAVYAALWIVLLPQFVPAGIDALPGGRLWMFV
                     CIAVLTAVAILGDLFESALKRQAGVKDSSQLLPGHGGVLDRVDALLPVLPLAALVVSL
                     "
     gene            complement(11169..11984)
                     /gene="uppS"
                     /locus_tag="VKZ48_02085"
     CDS             complement(11169..11984)
                     /gene="uppS"
                     /locus_tag="VKZ48_02085"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008350084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HLU76675.1"
                     /translation="MFTSPTQAIPESGAIPRHVAIIMDGNGRWAKQRFMPRIAGHRRG
                     AEAVRATVRACAERGIGYLTLFAFSSENWRRPQEEVSMLKQLFRAALENEVAKLHENG
                     IRLRVIGDIEPFGEDIVKLAREGEALTADNTRLTLTIAANYGGRWDLLQAMNRMVTAH
                     PECAGRYTEADLAPYLSMNYAPEPDLFIRTGGEQRISNFLLWQLAYTEFYFTDLLWPD
                     FNAGALDRAIASYQQRERRFGRTSEQVAQATRTVGGQPASVVELPDPRRKLAS"
     gene            complement(12050..12610)
                     /gene="frr"
                     /locus_tag="VKZ48_02090"
     CDS             complement(12050..12610)
                     /gene="frr"
                     /locus_tag="VKZ48_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HLU76676.1"
                     /translation="MDPASIKKSTEQKMQKSIEALKADLAKIRTGRAHTGLLDHITVD
                     YYGNPTPINQVANVTLVDARTINVQPWEKKMVAVVEKAIRESDLGLNPATHGESIRVP
                     MPPLTEERRRDLIKVVKAEAENARVAVRNLRREANQQLKEELKAKTISEDDDRRGQDD
                     IQKLTDRYIAEIDKLLQQKEAELLQV"
     gene            complement(12672..13388)
                     /gene="pyrH"
                     /locus_tag="VKZ48_02095"
     CDS             complement(12672..13388)
                     /gene="pyrH"
                     /locus_tag="VKZ48_02095"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HLU76677.1"
                     /translation="MPQPRHKRILLKLSGEALMGDDPFGINRDIVESIVAEIAAVHEL
                     GVEIGVVIGGGNIFRGVALGAAGMDRATADYMGMLATVMNALALADVMRLKGLNARVQ
                     SAINIEQVVEPYIRPKAIRYLEEGKIVVFAAGTGNPFFTTDTAAALRGSEIGAEMVLK
                     ATKVDGVYTADPMKDPGARRYDKVSFDEAIARDLKVMDATALALCRDQKLPITVFSLY
                     KPGALKRIVMGENEGTLVHV"
     gene            complement(13406..14284)
                     /gene="tsf"
                     /locus_tag="VKZ48_02100"
     CDS             complement(13406..14284)
                     /gene="tsf"
                     /locus_tag="VKZ48_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HLU76678.1"
                     /translation="MAEITAAMVKELRELTGLGMMECKKALTETDGDLKKAEDLLRIK
                     SGAKASKAASRVAAEGVIGAYLSPDAKLGALVEVNCETDFVAKNEDFLAFAKALAETV
                     AKENPADVDALSKLEAGGENIETRRQALVQKIGENIAIRRFVRMQTDAKLAQYLHGVK
                     IGVLVELEGDDDLGKDLAMHIAFAKPQYLSKDQVAPEVIEREREVLAARAAESGKPAE
                     IVNKMVEGGLNKFLAEITLLGQPFVKDDKQTVEKVLASRKAKLRNYKFFVVGEGIEKK
                     VTDFAAEVAAQAGVSK"
     gene            complement(14437..15183)
                     /gene="rpsB"
                     /locus_tag="VKZ48_02105"
     CDS             complement(14437..15183)
                     /gene="rpsB"
                     /locus_tag="VKZ48_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HLU76679.1"
                     /translation="MSTTMRQMLEAGVHFGHQTRYWNPKMAPYIFGHRNKIHIINLET
                     TLVMYNEAMKYVRQLAANKGTILFVGTKRSAREIVREEATRCGMPYVDHRWLGGMLTN
                     FKTVKQSIKRLKEMEQAVEDGSVERMSKKEGLLFTRELEKLQRSIGGIKTLDALPDAL
                     FVIDVGYQKGAVEEARKLGIPVVAVVDTNHSPEGLAHVIPGNDDSARAIRLYCRGIAD
                     AVLEGRSQTINEIIEAGSDDFVEVEESAES"
     gene            15465..16286
                     /gene="map"
                     /locus_tag="VKZ48_02110"
     CDS             15465..16286
                     /gene="map"
                     /locus_tag="VKZ48_02110"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HLU76680.1"
                     /translation="MAVVIKTAEEIEGMREAGRLAAEVLDFITPHVKTGVTTGELDRL
                     CHEYMRDVQGTVPAPLNYAPPGYPPYPKAICTSVNDVVCHGVPNEYALKDGDIVNLDI
                     TVITKDGFHGDTSRMYYAGEPSIQARRLCDVTLECMWIGIMKIKPGAYLGDVGHAIQR
                     YAESRGYSVVREFCGHGIGRKFHEEPQVLHYGRPGTGLKLQPGMIFTVEPMINAGKPG
                     IRELKGKRADGWTILTQDRSLSAQWEHTVLVTETGYEILTVSAGAPPIPQSLQAA"
     gene            16288..18864
                     /locus_tag="VKZ48_02115"
     CDS             16288..18864
                     /locus_tag="VKZ48_02115"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HLU76681.1"
                     /translation="MASVPARLPDAAAGAARQRLGNGRAALAAAFAADHDVARYLRGH
                     RQLIDSLLREAWKAAAMPRDCALVAVGGYGRGDQFRGSDVDVLVLLSAPPDAPTAQAV
                     ERFVGRLWDLGIELGHSVRTIAQCNEEAEKDITVRTALLEARRLCGDRTLFRAFQAAF
                     AERMDARSFFRAKLLEQQQRHAKFHDSPYSLEPNIKEAPGGLRDLQTILWTSRAAGFG
                     TTWLDLAERGLITRGEALSLGRQENFLKGLRLRLHLLAGRREERILFDYQDALARQYG
                     VTARDGRRASEVFMQRYYRAAKAITQLNTILIQNMEAALFPDSGEAAEPINERFQRRR
                     DLLHARDAQLFEREPAAILESFLLLQQHPELRGMTAPTLRALWRARQRIDAKFRRDPA
                     RRAQFLQILQQPRGILAALRRMNQYSILGRYLPAFGDIVGQMQHDLFHVYTVDQHILA
                     VVRNLRRFTLLEYVHEHPLCNSLMGQFERNWVLYIAALFHDIAKGRGGDHSKLGMRDA
                     AQFCRAHGLRADDTALVVFLVEQHLTMSNTAQKRDLSDPDVAREFAALVQTERRLIAL
                     YLLTVADIRGTSPKVWNAWKGKLLEDLFHATRRLLTGRDETVGQDAHTRQTEALRLLR
                     LYALPDEYSERLWRELDTTYFMRNEPQEIAWQTRSLHWRVRTERPVVKARLSPFGEGL
                     QVFVYGPADPDLFARICGYFESINFNIVDAKIHNTRHGYALDSFVVLGMDTSHHYRDI
                     INLIEYELSERLERRAPLGPPARGRVSRQLKHFPIAPEIHIRPDDKGQYHYLSVIAGD
                     RPGLLYRLARVLSRHEIALHSAKIITLGERAEDVFVVSGAALENEKGVLQLEQELLKA
                     LE"
     gene            complement(18868..19398)
                     /gene="def"
                     /locus_tag="VKZ48_02120"
     CDS             complement(18868..19398)
                     /gene="def"
                     /locus_tag="VKZ48_02120"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HLU76682.1"
                     /translation="MIREVLRMGDPRLLQQAQPVEQFDTPELHALIQDMRDTMAHLDG
                     AGLAAPQIGVGLQVVIFGVKRNARRPDIEEVPETVLINPNLTTIGSEMIEAWEGCLSV
                     PGMRGLVPRYAHLRYQGFDERGNPIDRTVEGFHAIVVQHEVDHLHGILYPMRIRDLAN
                     FGFADVLFPGEEQAGD"
     gene            complement(19423..20163)
                     /locus_tag="VKZ48_02125"
     CDS             complement(19423..20163)
                     /locus_tag="VKZ48_02125"
                     /EC_number="2.4.2.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioinosine phosphorylase"
                     /protein_id="HLU76683.1"
                     /translation="MLAIIGGSGLTTLSNMEITHRKAVRTPYGDPSGALTFGKMRGRE
                     VVFLARHGYGHTIPPHEVNYRANLWALQQEGIKQVVSVASVGGIREDLKPGTLVVPDQ
                     ILDYTWGRPSTYFQGGDAAVVHVDFTIPYAEPLRQCILRAAEGCGESIVDGGVYAATA
                     GPRLETAAEIRRYERDGADMVGMTGMPEAVLARELELHYATIAVVVNHAAGKGDSAKR
                     INFGEIESTLQQTIARARRVLEELVNAL"
     gene            complement(20216..20770)
                     /locus_tag="VKZ48_02130"
     CDS             complement(20216..20770)
                     /locus_tag="VKZ48_02130"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HLU76684.1"
                     /translation="MKTIEQAWRMLNDADLVCSAEDVERAIGRLGDEITARFHDRFPL
                     LLCVMNGALFFCGKLMPHLRFPLTLDYVHASRYGQDIDGRDIRWKVEPPVHVRDRAVL
                     VLDDILDEGVTLAAIKDRVLARGALSCDIAVLTDKRTGRVKPVRADFVGLEVPDRFVF
                     GCGLDAYGLWRNLPAVYALKEDTD"
     gene            complement(21008..21877)
                     /gene="galU"
                     /locus_tag="VKZ48_02135"
     CDS             complement(21008..21877)
                     /gene="galU"
                     /locus_tag="VKZ48_02135"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HLU76685.1"
                     /translation="MPSITKAVFPVAGFGSRFLPATKASPKEMMPVVDKPLIQYAVEE
                     AVAAGCTEMIFITGRHKRAIEDHFDKAYEIENELASRGKQELLDLVQSILPKHVNCIY
                     IRQPEQLGLGHAVLCAQPIVNDEPFAVILADDLIDAEKPVMKQMAEVFTREGRSVLGV
                     MEVPREQTGAYGIVRATSVDDRLESIEGIVEKPKPADAPSNLAVIGRYVLTPRIFHYL
                     RTIKPGAGGEIQLTDAIAALLNEERVLAYRFEGTRYDCGSKLGYLQATVAFGRKHAEV
                     GAEFDEWLKASRE"
     gene            complement(21883..>22105)
                     /locus_tag="VKZ48_02140"
     CDS             complement(21883..>22105)
                     /locus_tag="VKZ48_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HLU76686.1"
                     /translation="KTFVLTGTLPGMTRDEAKALIEAHGGKVAGSVSKKTGYVVAGAE
                     AGGKLAKAEALGVPILDEAGLINLLQQKG"
BASE COUNT         3874 a         7309 c         6863 g         4059 t
ORIGIN      
        1 ctggagcgcc gcgtctacta gcgccccgcc cgcagtcagg cgttcagcgt ctgccggacc
       61 tgctcgtaca ggcacaccgc cgctgccgcg gccacattga gcgactcggt accgccgggc
      121 atggggatgc ggatgcgcgt cgtggcgcag gcggccaggt cgggtgtcac gccggcacct
      181 tcgctgccga aaacccaggt caccgggccg cgcagatcga gttcataaag gctgcgttcg
      241 gcctgcagat cggtcacgat cacctccccc tcgaacgcct gcgcaaattc gaccagcggc
      301 gcggattcgt acagcttcag gccgaaatgc gcgcccatgc ccgcgcgcag caccttggcc
      361 gaccagacga aggcgcagcc cttggacaac agcacattgc gcaccccggc ggccgcagcc
      421 gtacgcagga cggagccgac gttccccgca tcctggatgc cctccaacag aaggcaggtc
      481 tcgtccgatg gcggcccttg ctcgtggacc ggcgtagcga tcagcgccac gatgccgctg
      541 ggggtgacga ccgccgcgat gttctgaaac aggcgatcgg tcagcaccac cttgctgacc
      601 gccggcgtcg tttcgaaaag ccgcgcgatc tctgcgcgct gcagaccgct ttcgctcgca
      661 atcaaagact cgacgcgccc gccgcttgcg ccataggctt cgatgagatg cgggccgtcg
      721 agcaaggcct gccgacgcgc gcgacgctcc ttggcggagt tgctcagctt cagcagcgac
      781 ttgaacagcg gattgtcgcg cgactgtatc cgtttgaccg acatcgccta gaacagctcg
      841 acctgctcgg gttcgccatc cgtcatgaga cggatcggcg caaagctgcg ccgatgcagt
      901 tcgcagggac cgtggcgccg cagcgattcg agatgctcgg gcgtggcgta gcccttgtgc
      961 tgcgcgaaac cgtaatgcgg gtagcgctcg tgccattgct gcatctctgc gtcgcgcgcc
     1021 gtcttggcaa ggatcgacgc cgcgccgatg cagcgcacct tggcatcgcc gccgacaatg
     1081 gcgcgcaccg gataagtcag cttgggacag cggttgccgt cgaccagcac ttccgccggc
     1141 gcaacgccca acgcctgcac ggcacgctgc atcgcgagca tggtcgccca gtagatgttc
     1201 aaacgatcga tttcttccac cgtggcaaat gcgacggacc aggtcagcgc ccgcaccttg
     1261 atgcgcgcgg caaggctttc gcgatcgcgt gcgctcagtt gtttggaatc gtcgatgccg
     1321 cgaatgcgtc gcgccggatc gaggatgacg gccgccgcat agaccggtcc cgcgagcgga
     1381 ccgcggccgg cctcgtccac gccgcagacg agcggacgcg ccgtgctcat gctgcccctc
     1441 ccgccaacga gcgttcgatc aacggtgcaa gcgcctcggc ggagcgctcc gccgcgcccc
     1501 gcttcatcac cgcgtgtacg gcagcaaagc gttcgcggat cgccaacagg acgcgcttgt
     1561 cctcatagag gttcagcgcc gcctgcgcca ggttttccgg cgtcacatca tcctgcagga
     1621 attcaggcac gacccggtcg tcgcacagga tattgggcag ccccacatgc ttgacgcgga
     1681 tgatgcgctt ggcgatggca aaactcatcg gcgacatccg ataggcgatg atcatcggac
     1741 agcgcagcag cgccgcttcg agcgtgacgg tgccgctggc ggcaatggcc agatcgctcg
     1801 ccgccagcgc ttcctgcgca tggccgaaca tcatgcgcac cggcaggtcg tgttcgccat
     1861 gctggtacaa cgcctgctcg aacaactcgt aggtttcgcg ggttgcacac ggcacgagaa
     1921 actgcacatt gccgaggcgc tgccggatca gtttggccgc gccgatgaaa ggctcggcgt
     1981 gattcatcac ctcctgctgg cggctgccgg gcagcaaggt gattaccgtg ctcttggccg
     2041 gcaggcgcag atattccagc agcgccccgc gatcgatggt gtccggaatc tcatcggcca
     2101 gcggatgacc gacaaagctc acgggtacgc cggcatcctg gtagatcgcc tcctcgaaag
     2161 gcaggaggac cagcatatgc gaaacggctt cgcggatcgt tttgatacgc cccttgcgcc
     2221 aggcccaaac ggttgggctg acgagatggg ccgtggtcac gccgcgtttg cgcagtcgct
     2281 tggcgagacc gagattgaaa tcgggcgaat cgatgcccag gaacagatgc ggccggctcg
     2341 ccgcaatgcg gcgcaccagt tcgcgccgga tggccatcag gccgcgcaga tgcttgagca
     2401 cttcgaccag gccgtgcacc gcgagacgct cctgcggaaa ccaggaatcg aaacccttgc
     2461 cgatcatttt cggcccgccg atgccgtaga actcggcatg gggatagcgc gccttcaacg
     2521 ccgcgatcac gcggctgccg agcagatcgc ccgaagcctc tccggcgacc atcgcaatgc
     2581 gaaccggggc gccgttcatc gcagcctcag cgcaggatgc cgcgcttggc ctgccgtagg
     2641 aactcgacca ggatttccag ttccggccgc tcctcctgct gctgcgccaa cgtctcgatg
     2701 gcttcggcca gcgtgaggtt gttgcgataa agggttttgt acgcccggcg cagcgcggtg
     2761 atgccttcgt tgctgaagcc gcggcgtctc aatccttccg tattcatgcc gaaaggcttg
     2821 gcgggattgc cggaggccat cacataaggc ggcagatcct gaaacaggat cgtgccgccg
     2881 ccggtaatgg cgtgagcgcc gattttgacg aactggtgca cgtcggtcat gccgccgagg
     2941 atcgcccaat cgccgatatg acaatgtccg gcgacttgcg cgttgctcgc gaaaatcgtg
     3001 ttgttgccga tctggcaatc gtgcgcgatg tgcacatagc ccatgatcca gttgtcgtca
     3061 ccgagacggg taacgcctgc gtcctgcacc gtgccggcgt tgatggtgca gtattcgcgg
     3121 atggtgttgc gatcaccgat ttccacgcgc gtgggctcgc cggcgtattt cttgtcctgc
     3181 ggctcgccgc ccagcgaaca gaagggaaaa atgcggttgt cgcggccgat gcgcatatga
     3241 ccgtcgagca ccacatgcga gccgatccag gtgttgtccc cgatttcgac gtattcgccg
     3301 acgatgctgt acggcccgat gctgacattg gcgccgagct tggcgccggg atgaacgatg
     3361 gcggtgggat gaatcatggc tgtgcgagag tggcaatgag ttgaaaacga tacgcagccg
     3421 tgctcagccc tcccgcatgg tgcacatcat cttcgcctct gccgccacct tgccgtccac
     3481 cgtcgcttcg gcagcgaact tccatacgtt cttgatggtg cgctcgatgc gcgccgtaaa
     3541 tttgagctga tcgccgggcg tcaccgggct cttgaaccgc gcctcgtcga tgcccaggaa
     3601 atagaccagc gaatcgtcgc tgggcaggcg gttggccgtc ttgaaggcca ggattgccgc
     3661 cgcctgcgcc atggcctcga tgatcagcac gccgggcatg atgggcagct tggggaaatg
     3721 ccccgggaaa aacggctcgt tgaacgtgac gttcttgatc gcgacgatac gttcgccggg
     3781 cacgcactcc agcacgcgat ccaccagcag gaacggatag cggtgcggca ggtactgttg
     3841 gatttcgttg atgtccatgg tgctcattct ttgttctccg tgcccgacat gccccgctcg
     3901 atgcggcgca cgcgatcgtt cagcttgtcc agcccgcgca acagcgcgac cgtgcggcgc
     3961 cattcgcgtc ccggcgtggc gggaatgacg ctcagataca tgcccggctc ctcgatggag
     4021 cgcgtgaccc aggtgccgcc ggagacgatt acccggtcgg cgatgtgcag atggccgccg
     4081 atcatcgccg cgccgccgat caggcaatgc ttgccgatgg ttgtgctgcc cgcgatgccg
     4141 gcgcaaccgg caacggcggt atgggcgccg atgcggcagt tatggccgat ctgaatctga
     4201 ttgtcgagtt tgacgccctc ttcgatcacc gtgtcgtcca gtgcgccgcg atcgatggtg
     4261 gtgttggcgc cgatctccac atcgtcgccg atgacgacgc gaccgatctg cggaatcttc
     4321 agccagcgtc ccgcgtcctg cgcatagccg aagccgtccg cgcccagcac gacgccggaa
     4381 tggatgatga cgcggcggcc gattacgcaa tcgtcgtaga gcgtcacgcg cgcatgcagc
     4441 cggctgcctt ggccgatggt gcagcgcggg ccgacgacgc aatgcgcgtc gagcgcaacg
     4501 tcggggccga cgacggcatc cgccccgatg acgcaaccgg cgccgacggc tgccgacgga
     4561 tcgatgcttg cggacgcatc gacaatcgcg gtgggatgca cgcccggttc gatcgccggc
     4621 tcgggattga gcagcagcga aacacgagcg aaatacagat aggggtcgtc gcagacgatg
     4681 cgcggccgcg tcgtcgcgtc gcgctccgcg gcgctgacga tcacggcgcc ggcctgggtc
     4741 gcgagcaact cgtcgcgata cttgccctgc gtgaagaagc tgatgtggtc ggggccggcg
     4801 ctacgcaggg tcgccacttg cgagatgcgc gtctcggcat cgccgatcac ctcgcccccg
     4861 agacgggcga cgaggtccgc cagccgcacg gtccgcgctt gcatgagttt ccttacttgg
     4921 cgcccttgcc gtcgtcgagc gacttgatca ccttgtccgt gatatcgatg cgcgggctgg
     4981 cgtatacggc ttcctggaaa atgatgtcga acttctcgga ttcggcaatc tgcttcaccg
     5041 cgcggttggc acgctcgagc acgccggcaa gctcttcgtt gcggcgctga ttgaggtctt
     5101 cgcggaactc gcgctgcttg cgctggaact cgcggctcgc ctcggccagt tcgcgttcgc
     5161 gacggctgcg ctcgctctcc tgcatggtca tggcattgcg ctccagcgcc tcctgcagct
     5221 tcttcacttc ctcggccatg cgctggagtt cctgatcgcg cttggtgaac tccgcttcga
     5281 tgcgcttctg ggcgcgctgt gccggtacgg cgtcccgcag gatgcgatcc aggttcacga
     5341 aaccgatctt gccctcctgg gcgaacgtcg gcacggccag cgccatcagc ggcaacaatg
     5401 cgagccaacg gaatttcatt aaggtcatgt ctgatttcct gtggtttgtg cggcggtcag
     5461 aacgcctgtc cgaacatgaa ctggaacttc tgcgtgtcgt ccccatcctc gtcgttgagc
     5521 ggcatggcca ggatgagctg cagtgggccg aatggcgaat tccaggacac aaagccgccc
     5581 gtagaatacc gcaacttcaa gtcgctcaag gtattgcccg agccggtatc ccaaacctgg
     5641 cctgcgtcga agaacacgcc catgcgcacc gaacggtcct gttgcatgcc gggcatgggg
     5701 aacaacagct cggcattacc gacggccttg cgcgagccac ccagcacgcc gtcgcggtcg
     5761 cgcgggccca gcgacccctg ctcgaaaccg cgcacggagt tcggaccgcc cccgaagaaa
     5821 gccttgaaga acggcagttc gccgccgccg tagccatccg catagccgac ctcgcccttg
     5881 aggcgcaggg tgagatcgcg cgacagcggc acataccagc tcaactcata gttgagcttg
     5941 tagtacttca aatcgacgcc cggcaggccg gtctcgagat aggcgcgctg ataggtgccc
     6001 gtcgtcgtcc agaaggcgct gtcgcgccga tcgcgggtcc agccgaccgt gcccagcaga
     6061 ccgagggtct tgtcgccctg tgaatcgacg aagtccttga agcggtccgg actgtcgtcg
     6121 aacagctcga tctcggtctg ttcgatcgcc aggccgaagt tgatccggtc gaattccgtg
     6181 acgggcacac ccacacgcaa gccggcgccc agcgtatcgg tggcgtagcg gccgaaactc
     6241 aagcggcggc gcggatcgta gcggcggctg taaagatcga agcctccgct cacgccgtcg
     6301 acggtcagat aggggttggt gaacgacagc ccgatgttac ggttgatctt gctggtgttc
     6361 atcatgatct gcagcgcgtt gcccgtgccg aacaggttct gctgcgacac cgagccctgc
     6421 agcaccaggc cgtccgcgct ggagaagccg gcgccgaaca tcagcgcgcc ggtgggtttt
     6481 tcggtcacgc tgtaggtgac gtccacctga tcggtggtgc cggccacggg ctgcgtgtcg
     6541 acggccacct ccgagaagaa atcgagtcgg tcgatgcgct gccgcgacaa ctgcagtttc
     6601 tcgccgtcgt agtacgcgcc ttcaagctgc cgcagttcgc gccgcacgac ttcgtcgcgc
     6661 gtcttcgtgt taccgaccac gtcgatgcgt cgcacgtaca cgcgccggcc ggggtcgacc
     6721 atgatgttga acgcgacggt gcgcttttcc ttgtccacgt ccggcaccgc gttggcgttg
     6781 gcgaaggcat agccttcgtt gcccagacgg tcggtgatgg ccttggtcga gtccgtcagc
     6841 ttctgccgcg agaacgcctc gcccggcttg atctggatga gcttgcgcag ttcctcttcg
     6901 ggcacgatgg tgttgcccga cagggtgacg tccgtgacgg tgtacttctc gccctcgacg
     6961 atgttgacgg tgatgtagat gtcgcgcttg tccggcgtga tcgatacctg cgtcgattcg
     7021 atgttgaagt cgagatagcc gcgatccaga tagaaggagc gcagcgtctc caggtcggcg
     7081 gacagcttct ggcgcgaata ctggtcgttc ttggtgtacc aggtgaacca gccgggcgtg
     7141 cgcagcacga acaggtcgag cagcgtttcc tcggtgtagg tctggttgcc gacaatgttg
     7201 atgcgcgcga ttttggcgac gtcgccctcg tccacggcga agttgatcgc cacgcggttg
     7261 cgatcgagcg gcgtcaccgt ggtctggatg gtcacgccgt agcggccgcg ggtcaggtac
     7321 tgacgcttga tctcctgttc ggccttgtcg atcgtggcct tatcgaagat gcggccttcg
     7381 gcgacattgt tctcgcgcaa agccagcttc acatcctcgg cgggaaattc cttcatgccg
     7441 ctgaagtcga tctgcgcaat cgccggccgc tcctccacgg cgacgaccag gatgttccct
     7501 tcgacttcga tgcgcacatc cttgaagaaa ccggtgccga acagcgcgcg gatcgcttcg
     7561 gtcgccttat cctgggtcat cgtttcgccg acgcgtatgg gcaggtaact gaataccgta
     7621 ccggcttcga tccgctgtat cccctccacc cggatatcgc gaacgacgaa gggatcgaat
     7681 gcgtgcgcag catgcgcgaa cagggcaccg atcagtgccg cagcgagctt ctttttcatg
     7741 ttgaggcggt gttcctcgtc agccggtgag cagccggctt atatcgttat aaaaggcaaa
     7801 caccatgagt gcgagcagta cggcgaggcc ggcgcgctgg cctatttcca taatccgctc
     7861 ggagaccgga ctgcctttca aaatttcggc cgtatagtac atcaggtgtc ccccatcgag
     7921 catcgggatc ggcagcagat tcagcacgcc gaggctgacg ctgatgagcg ccagaaaggc
     7981 cagataggga gccaggccca attgggcgga ttggccggcg taatcggcga tcgtcaccgg
     8041 gccggaaagg ttgcgccacg acacctcccc catcaccatc ttgccgagca tgcgcagcga
     8101 gaaaatcgac acgtcccagg tcttttgcgt cgcgcgcacc agcgcctcgc cgaggccgta
     8161 gcgaatatcg acgaagtatt cctgcatgcg cgcctcgtcg atccgcggtc cgacgcccag
     8221 ccggccgtaa cgcgcgtcgc cttcgcccac cgcttcgggc gtggcatcga gttgcaggcg
     8281 cagatcgccg cgcatcacgg tcagcgtaag ctgccgcgcc ggcgccgcgc gcacgcgctc
     8341 gaccagcatc tcccaatgcg caacaggctc gccgtcgatg gcgacgatgt gatcgtcctg
     8401 ctgcagacct gcccgctcgg cggcactgcc gggcgcgact tgaccgacca ccgcttcgat
     8461 ttccggccgg tgcggacgca ggccgatctg catcagcaga tccccgccgc tctggtctgc
     8521 cgtgacggtg gagagatcca gcacgcggcg ctcgaggctc cgcccggccg tcaacacttc
     8581 aagggtgccg ggcgcccggt cgatggcgtg ttcgagcagc agccagcgca catcctgcca
     8641 gctgcgaacc ggtacaccgt tgaccgtgcg gatgagatcg ccatcgcgca cgccggctgc
     8701 ctcggcgagc gaatgcgccg acggcgtgcc ggcgatcggc ttgagttcct gcacgccgcc
     8761 gacgtatagc gtggcgtata gcgcaatggc gagcaggaaa ttcgccgccg gcccggccac
     8821 tacgatggcg atgcgcttcc agacgttctg ccggttgaag gcacgcggca agtcggccgc
     8881 cggcacctcg ccttcgcgct cgtcgagcat cttcacgtag ccgcccagcg ggatgggcgc
     8941 caatgcccat tcggtctgat cgcgacccag cctgcggatg tagaacggcg tgccgaagcc
     9001 gaccgaaaag cgcagcactt tcacgccgca ccaacgtgcc accagatagt ggccgaactc
     9061 gtgcacgacg acgaggaggc ccagcgcgag gatgaaggag aggaccgtgt acagcagcgt
     9121 catgcggttt tcgccagcga gcggccgcgc accagcgcag acgcttcgag tcgcgcgcga
     9181 cggtcggcct cggtgacgtc ctcgagcgat tcgatgcgct gcgcgggcag ccgttcgagc
     9241 gtcgcctcga tcacttcggt aatgcgcggg aaggaaagtt cggcattgag gaaggcttcc
     9301 accgccactt cgttggcagc gttgaagacc gcgggcgccg aaccgccggc tgtcagtacg
     9361 tcataggcca gggccaaagc gcggaaacgc tgaaaatccg gctgctcgaa attcagtgtg
     9421 ccgatcttgg caagatcgag aaaacccacg cccgcatcga cgcgctccgg atacgccagc
     9481 gcctgggcga tgggggtgcg catgtcgggg tggcccaact gcgcgatcac cgatccgtcg
     9541 gcatattcga ccattgagtg caccacgctc tgcggatgga tgacgacttc gatctgctgc
     9601 gcgggcgcgc cgaacagcca atgcgcttcc accacttcca gcgccttgtt catcatggtg
     9661 gccgaatcga ccgaaatctt gcgccccatg ctccataccg gatgggcaca ggcctgatcc
     9721 ggcgtgatct gcgtcagttc cgccaacggg cgcgtacgga acggaccgcc gctgccggtc
     9781 agcaggatgc gacgcacgcc gcaatcggtc agtctgccgg aggccaatgc cggcaggcac
     9841 tggaagattg cgttgtgctc gctgtcgatg ggcaacagcg tcgcgccgcc cgcacgcact
     9901 gcagccatga agatcgggcc cgccatcacc agcgcttcct tgttggcaag caggatcttc
     9961 ttcccggccg aggccgcagc aatggtgggt ttgagaccgg ccgcaccgac gatggccgcc
    10021 atgacgctat ccacatccgg atgcgtcacg gcctcgacca gcccctgttc gccgctcagc
    10081 accgcaatgt cgatgccgtc cgcctcgaaa cgggcgctca gcgcgcgggc gcccgcggcg
    10141 tccgccacca cggccacggc gggccgatgc acgcgacacg cctcatacag cgcatcgata
    10201 ttgcgatgcg ctgtgagcgc atgaatcctg aagcgatcgg ggtgtcgggc aaccacgtcg
    10261 agcgtgcttc ggccgatcga gccggtcgca ccgagtaccg caatgcgctg gacaaccgtc
    10321 atagcgaaac caccaacgcc gcgagcggca ataccggaag caaggcatcg acccggtcca
    10381 gcacgccgcc gtgcccgggc agcaactggc tgctgtcctt cacgccggcc tgccgcttca
    10441 gagccgattc gaacaaatca cccaggatgg caaccgccgt caataccgcg atgcagacga
    10501 acatccacag ccggccgccg ggcaaggcat cgataccggc cggcacgaat tggggcaaca
    10561 ggacgatcca caacgccgca taaacggccg tcgcaacgaa tgcgccgtaa acgcccgccc
    10621 aggtcttgcc cggactgatg gacggcgcga gtttccgcct gccgaagcgg cgtccggtga
    10681 aataggctgc gatatccgcg atccagacgg tggccatgaa cagtaacaag ctgacgggat
    10741 gtacctcacg caactgtacc agcgcggcgt aacagggcag cagcaccacg acaccggcca
    10801 gcaacagcag catcccggga agcttgtggg caggcgaacg caaccagaac ggcatgcaga
    10861 tcgcccagaa ggcggccgcc aggaggtagg ccatcgatac ggcgccgtat gaaatcgcgc
    10921 cgctgaggat gccggtcgca tagacccaac ccagtccgac cgcggtgagg accgcgccgt
    10981 acacgacccg ttgggccgac gcatagccgg cgaggcctgc ccattcccac gcggcgcaat
    11041 aaagcgcaat cccgatgaac agcgcccagc ccagcgccgg caggaagaac agaccaccga
    11101 gcagcacgcc gatcagaatc agcgccgtga tgacgcgatc gcgtaacacc tgccgccttc
    11161 cccgccgctc aggacgccag cttgcggcgc ggatcgggca attccaccac gctggccggt
    11221 tggccgccca cggtgcgcgt agcctgcgcc acctgctcgc tggtgcggcc gaaacggcgt
    11281 tcgcgctgtt gataggaagc aatggcccga tccagtgcgc cggcgttgaa atccggccat
    11341 agcagatcgg tgaagtagaa ctcggtgtaa gccagttgcc acaacaggaa gttgctgatg
    11401 cgctgctcgc cgccggtgcg gatgaaaaga tccggctcgg gagcgtagtt catcgacaga
    11461 tacggagcga gatcggcttc cgtgtaccgg ccggcgcact cgggatgtgc ggtcaccatg
    11521 cgattcattg cctgcagcag atcccagcgg ccgccgtaat tggccgcgat ggtcagggtg
    11581 agccgggtgt tgtcggcggt gagcgcctcg ccctcccgcg ccagcttgac gatgtcctcg
    11641 ccgaagggct cgatgtcgcc gatgacgcgt agacgaatgc cgttctcgtg cagtttggcg
    11701 acttcgttct cgagcgccgc gcgaaacagc tgcttgagca tcgacacttc ttcctgcggc
    11761 cgccgccagt tctccgaact gaaggcgaac agcgtcaggt agccgatgcc acgctccgcg
    11821 caggcgcgca ccgtggcccg caccgcttcc gcacctctgc gatgcccggc gatgcgcggc
    11881 atgaagcgct gcttcgccca ccggccattg ccgtccatga tgatcgcgac atggcgcgga
    11941 atggcgccgc tttcgggtat tgcctgggtc ggactggtga acatgcgttc ctcgacggcg
    12001 tggcacttga gtcgaaaacc ggccacgctg cggcgcagcc tcggcttcgt cagacctgca
    12061 gcagttcggc ttccttctgc tgcagcaatt tgtcgatctc ggcgatatag cggtcggtga
    12121 gcttctggat atcgtcctgc ccccgacgat cgtcgtcttc ggaaatcgtc ttggccttca
    12181 actcttcctt gagttgctgg ttggcttcgc gacggaggtt gcgcaccgcg acgcgggcat
    12241 tctcggcctc ggccttgacc accttgatga gatcgcgccg gcgctcttcc gtcagcggcg
    12301 gcatcggcac gcggatcgat tcgccgtgcg tggcggggtt gagtcccaga tccgattcgc
    12361 ggatcgcctt ttccaccacc gccaccatct tcttttccca cggctgcacg ttgatggttc
    12421 gcgcatcgac caaggtgacg ttcgcgacct gattgatcgg cgtcgggttg ccgtaatagt
    12481 cgaccgtgat gtgatcgagc aggccggtat gtgcgcgacc cgtgcggatc ttggccagat
    12541 cggccttcag cgcctcgatc gatttctgca tcttctgctc ggtcgatttc ttgatgcttg
    12601 cgggatccat cctcttatcc tcgattcttc agcccgcatg cgcgggcttg ctgtcaacgg
    12661 ggacacgccg ttcagacgtg gaccagcgtg ccttcgttct cgcccatgac gatgcgtttc
    12721 agcgcacccg gcttgtacag actgaacacg gtgatcggca gtttctggtc gcggcacagc
    12781 gcgagcgcag tcgcatccat gactttgaga tcgcgcgcga tggcctcgtc gaaactcact
    12841 ttatcgtagc gccgcgcgcc cgggtccttc atcgggtctg ccgtatagac gccgtccacc
    12901 ttggtcgcct tgagcaccat ttcggcgccg atttcgctgc cacgcaacgc cgctgcggta
    12961 tcggtcgtga aaaagggatt gcccgtaccg gcggcgaaga cgacgatctt gccctcctcc
    13021 aggtagcgga tcgccttggg ccggatgtag ggctcgacga cctgctcgat gttgatggcc
    13081 gactggacgc gtgcattgag gcccttcagc cgcatgacgt ccgccagcgc gagcgcgttc
    13141 atcaccgtgg cgagcatgcc catatagtcg gcggtggcgc ggtccatgcc cgcggcgccg
    13201 agcgcgacgc cgcggaagat gttgccgccg ccgatgacca cgccgatctc gacgcccaac
    13261 tcatgcaccg cggcgatttc cgccacgatg ctctcgacga tatcgcggtt gatgccgaag
    13321 gggtcgtccc ccatcagggc ttcaccgctc aacttcaaca gaatgcgctt gtggcgcggt
    13381 tgcggcatgg cgatctcgcg tcggtttact tgcttacgcc ggcctgcgcc gccacttccg
    13441 cggcgaagtc ggtcaccttt ttctcgattc cctcaccgac gacgaagaat ttgtagttgc
    13501 gcagcttcgc cttgcggctg gcgagcacct tctccaccgt ctgtttgtcg tccttcacaa
    13561 agggctggcc gagcagcgtg atttcggcga gaaacttatt gaggccgcct tcgaccattt
    13621 tgttcacgat ttcggcgggc ttgcccgact ccgcggcacg cgcagccagc acttcacgct
    13681 cgcgttcgat cacttcgggc gccacctggt ccttcgacag gtactgcggc ttggcgaagg
    13741 cgatatgcat ggccaggtcc ttgcccagat cgtcatcgcc ttccagttcc accagtacgc
    13801 cgattttcac cccatgcaga tactgcgcca gtttggcgtc ggtctgcatg cggacgaaac
    13861 ggcggatggc gatgttctcg ccgattttct gcaccagcgc ctgccggcgg gtctcgatat
    13921 tctcgccgcc ggcctccagc ttggacagtg cgtccacatc cgccgggttt tccttcgcga
    13981 ccgtctcggc caatgccttg gcgaaggcaa ggaagtcctc gttcttggcg acgaagtcgg
    14041 tttcgcagtt cacttccacc agcgcgccga gcttggcgtc gggcgaaagg tacgcgccga
    14101 tcacgccttc ggccgcaacg cggctggccg ccttgcttgc cttggcgccg ctcttgatgc
    14161 gcagcaggtc ctcggccttc ttcaggtccc cgtcggtttc cgtcagggcc tttttgcatt
    14221 ccatcatgcc cagcccggtg agctcgcgca gctccttcac catcgctgcg gtaatttcag
    14281 ccattccatg ctccttgatt cgatgcgcgc gccgcaagcg gcgcgtactc tttaacggat
    14341 cgcggtgcga tccgttgtca atctttgaaa aaaaggggca tacgcccctt tcgaatcgag
    14401 cgcttcgagc aagcgcgcgg cccgaaccgc ggccgctcag ctctcggcgc tctcttccac
    14461 ttcgacgaag tcgtcgctgc cggcttcgat gatttcgttg atggtctgcg agcggccttc
    14521 cagcaccgcg tcggcgatgc cgcggcagta cagacgaatg gcacgcgccg agtcgtcatt
    14581 accgggaatg acatgcgcca gaccttcggg cgagtggttg gtatcgacca ccgcgacgac
    14641 gggaatcccc agcttgcgcg cctcttcgac cgcacccttc tggtagccga cgtcgatcac
    14701 gaacagcgcg tcaggcagtg cgtccagcgt cttgatgccg ccgatgctgc gctgcagctt
    14761 ttccagttcg cgcgtgaaca gcaaaccttc cttcttggac atgcgctcga cgctgccatc
    14821 ctcgaccgcc tgttccattt ccttcagtcg cttgatggac tgcttgaccg tcttgaagtt
    14881 agtcagcatg ccgcccagcc aacgatggtc gacatagggc atgccgcagc gggtcgcttc
    14941 ctcgcgcacg atctcgcgcg cggaacgctt ggtaccgacg aaaagaatgg tgcccttgtt
    15001 cgccgccaac tggcggacgt acttcatcgc ctcgttgtac atcaccaacg tcgtttcgag
    15061 gttgatgatg tgaatcttgt tgcgatgacc gaagatgtag ggagccatct tcgggttcca
    15121 gtagcgggtc tggtgtccga aatggacgcc cgcctccagc atttgacgca tggtcgtaga
    15181 catggactgc tccgttttag ggttatcact gacgcccgcc caaccgaacc ccgccacgcg
    15241 gagcacccta aagggcggac gctgagattt gcgccgatcc ggcgcctcca ccgaacgccg
    15301 agttcatggc gcccaagtgg ccgcggagta tagcacgcac cccttgtaga ccccaacaat
    15361 tcgccttgcc gaacagacga ttaagcgcct cgccgccggc ggggaaattg cctccccgct
    15421 ccccatcctc tatccttctt cgttttcgaa aggctttgtg atccatggcg gtcgtcatca
    15481 agactgcgga agaaatcgag ggcatgcgcg aggccggccg actggccgcg gaagtgctcg
    15541 atttcatcac cccgcacgtc aaaacggggg tgaccacggg cgaactcgat cgtctgtgcc
    15601 acgaatacat gcgcgacgtg cagggcacgg ttcccgcgcc gctcaattac gccccgcccg
    15661 gctatccgcc ctatcccaaa gccatttgca cctcggtgaa cgacgtcgtg tgccacggcg
    15721 tccctaacga gtacgccctg aaggacggcg atatcgtcaa cctggatatc accgtcatca
    15781 ccaaggatgg tttccacggc gataccagcc gcatgtacta cgcgggcgag ccgtccatcc
    15841 aggcacgccg cctgtgcgat gtcacgctcg aatgcatgtg gatcggcatc atgaaaatca
    15901 agcccggcgc ctatctgggc gatgtgggtc acgccatcca gcgatatgcg gaatcgcgcg
    15961 gctacagcgt ggtgcgcgaa ttctgcggcc acggcatcgg ccgcaaattc catgaggagc
    16021 cccaggtgct gcactacggg cgcccgggca cgggtttgaa gctgcagccc ggcatgattt
    16081 tcaccgtcga gccgatgatc aacgccggca agcccggcat ccgcgaactc aagggcaagc
    16141 gcgccgacgg ctggaccatc ctcacccagg accgcagcct gtccgcgcaa tgggagcaca
    16201 ccgtgctcgt caccgaaacg ggttacgaga tcctcaccgt ctcggccggc gccccgccaa
    16261 ttccgcagag cctgcaagcc gcctgacatg gcctccgtgc ccgcccgcct gccagacgcc
    16321 gccgccggcg ccgcccggca gcggctcggg aacggccgcg cggcgctcgc cgcggcattc
    16381 gccgcagacc acgatgtcgc ccgctatttg cgcggccatc gccaactcat cgacagcctg
    16441 ttgcgcgagg cctggaaagc ggccgccatg ccgcgcgatt gtgcgctggt cgcggtcggc
    16501 ggctacggcc gcggcgacca gttccgcggc tccgacgtcg acgtgctcgt cctgctgtcc
    16561 gccccgcccg atgcacccac cgcgcaggcc gtggagcgtt tcgtcggccg cctgtgggac
    16621 ctgggcatcg aactgggcca cagcgtgcgc accatcgctc aatgcaacga ggaggcggaa
    16681 aaggacatca ccgtacgcac cgcgctgctg gaggcgaggc gtctgtgcgg cgaccgcacg
    16741 ctgttccgtg ccttccaggc ggcgttcgcc gaacgcatgg atgcgcgcag cttcttccgc
    16801 gccaagctgc tcgaacagca gcagcggcac gccaagttcc acgacagccc ctacagcctc
    16861 gagccgaaca tcaaggaagc gcccggcggc ctgcgcgatc tgcagaccat tctgtggacc
    16921 agccgcgccg ccggtttcgg gacgacctgg ctcgatctcg ccgagcgcgg tctgatcacg
    16981 cggggcgaag cgctgagcct cggacggcag gagaacttcc tcaaggggct gcgcctgcgc
    17041 ctgcacctgc tcgccggccg ccgcgaggag cgcattctgt tcgactacca ggacgcgctg
    17101 gcacgtcagt acggcgtcac cgcccgggac ggacgccgcg ccagcgaggt gttcatgcag
    17161 cgctactacc gcgcggcgaa ggcgatcacg cagctcaaca ccatcctcat ccagaacatg
    17221 gaagcggcgc tgttcccgga cagcggcgaa gcggccgaac cgatcaacga gcgcttccag
    17281 cggcggcgcg acctgctcca cgcccgcgat gcgcagttgt tcgagcgcga accggcggca
    17341 atcctggaaa gctttctgct attgcagcag catccggaat tgcgcggcat gaccgcaccg
    17401 acgctgcgcg ctctgtggcg tgcgcgccag cgtatcgatg cgaaattccg ccgcgatccg
    17461 gcgcgccgtg cgcagttcct gcaaattctg cagcagccgc gtggcattct cgccgcgctg
    17521 cggcgcatga accagtacag tattctcggc cgctacctgc ccgccttcgg cgacatcgtc
    17581 gggcagatgc agcacgacct gtttcacgtc tataccgtcg accagcacat cctcgcggtg
    17641 gtgcgcaacc tgcgccgttt cacgctgctc gagtacgttc acgaacaccc gctgtgcaac
    17701 agcctgatgg gccagttcga gcgcaactgg gtgctctaca tcgccgccct gtttcacgat
    17761 atcgccaagg ggcgcggcgg cgaccattcg aaactcggca tgcgcgacgc cgcgcagttc
    17821 tgccgcgccc acgggctgcg cgcggacgac acggcactgg tggtcttcct ggtcgagcag
    17881 catctgacca tgtccaacac cgcgcagaaa cgcgacttgt ccgacccgga tgtggcacgc
    17941 gaattcgccg ccctggtgca gacggagcgg cggttgattg cgctttatct gctgactgtg
    18001 gccgatatcc gcggcaccag cccgaaggtg tggaacgcct ggaagggcaa gctgctcgag
    18061 gacctcttcc atgccacacg gcggctgctc accggccggg acgaaaccgt cggtcaggac
    18121 gcgcacacac gtcagaccga agcgctgcgc ctgctgcgtc tgtacgccct gcccgacgaa
    18181 tacagcgaac gcctctggcg cgaactggac accacctact tcatgcgcaa cgagccgcag
    18241 gaaatcgcct ggcagacgcg cagcctgcac tggcgcgtac gcaccgaacg gccggtggtg
    18301 aaggcgcgcc tgtcgccctt cggcgaaggg ctgcaggtgt tcgtctacgg gccggcagac
    18361 ccggatctgt tcgcgcgcat ctgcggctac ttcgagtcca tcaacttcaa catcgtggac
    18421 gccaagatcc acaatacccg ccacgggtac gcgctcgaca gcttcgtcgt gctggggatg
    18481 gacacgtcgc accactatcg cgacatcatc aacttgatcg aatacgaatt gagcgaacgg
    18541 ctggagcggc gcgcaccgct cggcccgccg gcgcgcggac gcgtatcgcg gcagttgaag
    18601 cacttcccca tcgcgccgga aatccacatc cggcccgacg acaaaggcca gtatcactat
    18661 ctctccgtca tcgcgggaga tcggcccggt ctgctttatc gtctggcgcg ggtattgagc
    18721 cggcacgaga tcgcgctgca ctcggccaag atcattacgc tcggcgagcg ggcggaagac
    18781 gtcttcgtgg tgagcggcgc cgcgctggag aacgaaaaag gcgtgctgca gttggagcag
    18841 gagctactga aggcgctgga gtagccctca gtcgcccgcc tgttcttccc ccggaaacag
    18901 cacgtcggcg aaaccgaagt tggccaggtc gcgtatgcgc atgggataaa ggatgccgtg
    18961 cagatggtcg acctcgtgct gcacgacaat ggcgtgaaag ccctcgaccg tgcggtcgat
    19021 gggattgccc cgctcgtcga acccctgata acgcagatgc gcatagcgcg gcaccaggcc
    19081 ccgcatgccc ggcacggaca ggcagccctc ccaggcttcg atcatttcgc tgccgatggt
    19141 ggtgaggttg gggttgatca gtaccgtttc cggcacttct tcgatatcgg gtcggcgtgc
    19201 gttgcgcttc acgccgaaaa tcaccacctg caggccgaca ccgatttgcg gcgcggccag
    19261 tccggcgcca tccagatgcg ccatggtgtc gcgcatatcc tggatcaacg catgcagttc
    19321 cggtgtgtcg aactgttcca cgggctgggc ctgctgcaac aaccgcggat cacccattct
    19381 caggacttcg cgaatcatgt cggctcctgt tgcgatgagg cgctacaagg cgttcaccag
    19441 ttcctcgagg acgcgtcgcg cgcgcgcgat agtctgctgc aatgtcgatt cgatctcgcc
    19501 gaaattgatg cgcttggcgc tatcgccctt gcccgccgcg tgatttacca ctacggcaat
    19561 ggttgcgtaa tgaagttcca gttcgcgcgc caggaccgcc tcgggcatgc cggtcatgcc
    19621 gaccatgtcc gcgccgtcgc gctcgtaacg cctgatctcc gccgcagttt ccagccgcgg
    19681 cccggccgtc gccgcataga cgccgccgtc cacgatggat tcgccgcagc cctccgccgc
    19741 gcgcaggatg cactgccgta gcggttcggc gtagggaatg gtgaaatcca cgtgcaccac
    19801 cgccgcatcg ccgccctgga aataggtgga cggccggccc caggtgtaat cgaggatctg
    19861 atccggcacg accagcgtgc ccggtttcag gtcctcgcgg atgccgccca ccgatgctac
    19921 cgacaccacc tgcttgatgc cttcctgctg caacgcccag aggttggcgc ggtaattcac
    19981 ttcgtgcggc ggaatggtgt ggccgtaacc gtgacgcgcc agaaacacca cctcgcgacc
    20041 gcgcatcttg ccgaaggtca gcgcgccgga agggtcgccg tagggggtac gcaccgcctt
    20101 gcggtgcgtg atttccatat tgctcagcgt ggtgaggccg ctgccgccga tgattgccaa
    20161 catgattctt cgatctgccg tacttgattg gttaaaggac tgccggccca aggcctcagt
    20221 cggtgtcttc cttgagcgca tagaccgccg gcagattgcg ccataacccg taggcgtcca
    20281 acccgcagcc gaacacgaaa cgatccggga cttccagtcc gacgaagtca gcccgcacgg
    20341 gcttgacgcg cccggtgcgt ttgtcggtca gcacggcgat atcgcaggac agggcgcccc
    20401 gggcgagcac gcgatccttg atggcagcga gtgtgacgcc ttcgtcgagg atgtcgtcca
    20461 gcaccagcac cgcccgatcg cgtacatgca ccggcggctc gactttccag cggatatcgc
    20521 gtccgtcgat atcctggccg tagcgcgacg catgcacgta atcgagggta agcggaaagc
    20581 gcaggtgcgg catgagtttg ccgcagaaga acagggcgcc gttcatgaca cagagcaaca
    20641 gcggaaagcg gtcatgaaag cgtgcggtga tctcgtcgcc cagacggccg attgcgcgct
    20701 ccacgtcctc cgcggagcac acgaggtccg cgtcgttcaa catccgccag gcctgttcta
    20761 ttgttttcat gccggtctcc cgccgtgctt ctcatgtggc ggagtatatg tcacgggatg
    20821 gcagtcggca gccggccggc catgctccaa aagccgaaac acggagacac ggagaaaggc
    20881 ggtgaagcct ggccctggtt cggcatttga agggcgccct caccgcgcag gcgcccgccg
    20941 cgctgcgagc cgcgcaccgc gacggcttac tccgcgctct ccgtatactc tgtggttccg
    21001 gctgtttcta ctctcgactg gctttgagcc attcgtcgaa ctccgccccc acttccgcat
    21061 gcttgcggcc gaacgcgacg gtggcctgga gataaccgag cttggagccg cagtcgtagc
    21121 gcgtcccctc gaagcggtag gccagcacgc gttcctcgtt cagcagggcg gcgatggcgt
    21181 cggtcaactg gatttcgccg cctgcgccgg gcttgatggt gcgcagatag tggaagatgc
    21241 gcggggtgag cacatagcgg ccgatgaccg ccagattgga aggcgcatcc gcgggcttcg
    21301 gcttctcgac gatgccttcg atcgactcga ggcgatcgtc gacgctggtt gcacgcacga
    21361 tgccataggc gcccgtttgt tcgcgcggca cttccatcac gccgagcacc gagcggccct
    21421 cgcgggtgaa gacctccgcc atctgtttca tcaccggctt ctccgcgtcg atcagatcat
    21481 ccgcaaggat gacggcgaag ggttcgtcgt tgacgatggg ctgcgcgcac agcacggcat
    21541 ggcccaagcc caactgctcc ggctggcgga tataaatgca gttgacgtgc ttgggcagga
    21601 tgctctgcac caggtcgagc agttcctgct tgccgcgtga cgccagttcg ttttcgattt
    21661 cgtaggcttt atcgaaatga tcctcgatgg cccgcttgtg acgaccggtg atgaagatca
    21721 tttccgtgca gcccgcagcc accgcctctt ccaccgcata ttggatcagc ggcttgtcca
    21781 ccaccggcat catttccttc ggactggcct tggtcgccgg cagaaagcgg ctgccgaagc
    21841 ccgctacggg gaaaactgct ttggttatgg aaggcattcc gttcatcctt tttgttgcaa
    21901 taagttgatc agtccggcct catcgagtat cggtacgccc agggcttccg ctttggcgag
    21961 cttgccgccc gcctccgcac cggctaccac gtagccggtt tttttcgata ccgagcccgc
    22021 caccttaccg ccatgcgcct cgatcagcgc cttcgcctcg tcgcgcgtca tgccgggcaa
    22081 ggtgccggtg agcacgaaag tcttg
//
LOCUS       DATGOU010000038         4552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_4529975, whole genome shotgun sequence.
ACCESSION   DATGOU010000038 DATGOU010000000
VERSION     DATGOU010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4529975"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..949)
                     /gene="gloB"
                     /locus_tag="VKZ48_02145"
     CDS             complement(179..949)
                     /gene="gloB"
                     /locus_tag="VKZ48_02145"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HLU76687.1"
                     /translation="MSKPTVVPIPAFNDNYIWALHDTRRVAIVDPGDARPVQDYLRRE
                     GLALSAILLTHHHNDHIGGVATLARAHDVPIYAPHDERIATATERVREGDTVELPEFG
                     LKFEVLEVPGHTRTHVAYYGAGMLFCGDTLFACGCGRLFEGTPEQMHRSLGKFAALPD
                     DTLVYCAHEYTLSNIAFSRAVEPDNAALAAREASDRASREANRPTLPSTIGLEKATNP
                     FLRVTEPAVTASAARYAGRDLGTPVDVLAAIRQWKNEF"
     gene            1064..1795
                     /locus_tag="VKZ48_02150"
     CDS             1064..1795
                     /locus_tag="VKZ48_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLU76688.1"
                     /translation="MSTLTDWFGTPKGAYVLEWELTQFDNAVEDVFGFIAVQVGSPKL
                     DFLRANRVPVRTCIGGPGCGVLGEPFALPLASQSVDLIVLPHVLEFSAMPHQILREAE
                     RVLRPEGHIVISGFNPLSLWGVKRMISHRRGEYPWSGDFIGLLRLRDWLKLLGFELNG
                     GRFGCYAPPFSTTKWLQRFSFMESAGDRWWPIAGGVYVVRAVKRVYGMRLLQPDWKNG
                     KKRTKKVLAPVAQRHDGVQRNGHER"
     gene            1785..2252
                     /gene="rnhA"
                     /locus_tag="VKZ48_02155"
     CDS             1785..2252
                     /gene="rnhA"
                     /locus_tag="VKZ48_02155"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HLU76689.1"
                     /translation="MNDKNNVVRLYTDGACKGNPGVGGWGVLMRYGDKEKELYGGEAM
                     TTNNRMELMAVIRGLNALTRPCTVEVWLDSEYVKNGITQWMTNWKRRGWKTADRKPVK
                     NAELWRELDEATQRHEITWHWVRGHTGHEGNERADALANRGVEEVRANLVSAR"
     gene            complement(2285..3709)
                     /locus_tag="VKZ48_02160"
     CDS             complement(2285..3709)
                     /locus_tag="VKZ48_02160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HLU76690.1"
                     /translation="MSLKRTLLCAVSLLALAGCAVGPDYERPALDLPMDYGAVADRSE
                     PNIPSDWWTLYGDPKLNELVEAGLTRNTDMKFAVARIEEAEAVLREARSLFFPTIDAN
                     GGASRGRSMAPATPGVSTVRNTFTLTATTAFELDFWGRLRRANEAAQAQYLATRYGRD
                     VVGLTLASAIAQTYFAVRSLQAQTEVAAENVRVSAESLDIATKRFEAGLISELDVNQA
                     QSAHAGARAQLKEVQRQRDVTLHQLGELTGILDLQLEPGDVRALPSPPVPPAGLPASL
                     LERRPDVRQAEAQLAAANAAIGVARAAQFPTFSLTGSYGRQSDTLSTLFESGNKIWSV
                     GLNLMGPIFDAGRYAARTDQAVAQQKQAAALYEQAAEAAFRDVKDALSNVAWTADMEA
                     DLLARVEAARNSLRLARLRYESGYSSFLEVLDAQRTLNEAELELVRNRQAYLSYTTDL
                     MEALGGGWTDTDYPPTAQSAPAKD"
     gene            complement(3706..>4552)
                     /locus_tag="VKZ48_02165"
     CDS             complement(3706..>4552)
                     /locus_tag="VKZ48_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLU76691.1"
                     /translation="VQLQADAPYRAQPEDLGRIHVRSNTTGEMIPLKTFLTVKPIVGP
                     EQIERFNGFLGAKVMGSGKPGVSSGTAIAAVEQVAAEVLPPGFTIAWTGQAYQEKGTG
                     SASIFAFGFALVMVYLILSALYERWRLPSAVLLAVPFAIVGALGLVWLRGMENDIYFQ
                     IGLVVLIGLSAKNAILIVEFAQQGLLAGKKPMEAALEAARLRFRPIVMTSLAFILGVL
                     PLAVASGAGSAARRSMGTGVVGGMLAATAIATIFIPLFFTLFASLRKADKHPDQASDE
                     LKEPA"
BASE COUNT          813 a         1407 c         1543 g          789 t
ORIGIN      
        1 atgccacggt cagaatggcg agttgtaaga aaaatcggcg cacgaaacct cccggaagtg
       61 ccgccgatgg tatggggacg gcgcgagaaa cgtcaaacct aaagttgcgc ggcaggtggg
      121 acagtgccgc taccggccat gcaaggcgag ggccgctgtg acacagttgt gcacaaattt
      181 aaaattcgtt cttccattgg cggatggcgg ccaggacatc caccggcgtg ccgaggtccc
      241 gaccggcgta tcgcgccgcc gaggcggtta ccgcgggctc ggtgacgcgc aggaagggat
      301 tggtcgcctt ttccaacccg atggtggagg gcagcgtcgg gcggttcgct tcgcggctcg
      361 cccggtcgct tgcttcgcgc gccgcgagcg cagcgttgtc cggttcgact gcacgcgaaa
      421 aagcgatgtt cgacagcgtg tattcgtgcg cgcaatagac cagcgtatcg tccggcagag
      481 cggcgaattt gccgagcgaa cggtgcatct gttcgggcgt gccctcgaac agccggccgc
      541 agccgcaggc gaacagcgtg tcgccacaga acaacatgcc tgcgccataa taggccacgt
      601 gcgtgcgcgt gtgtccgggc acttcgagga cctcgaattt cagaccgaac tcgggcaact
      661 cgaccgtatc gccttcgcgc acgcgttcgg tggcggtggc gatgcgctcg tcatgcggcg
      721 cgtagatcgg cacgtcgtgc gcgcgcgcaa gcgtcgcgac gccgccgatg tggtcgttgt
      781 gatggtgcgt gagcaggatc gcgctgagcg ccagcccttc gcgtcgcaag taatcctgca
      841 ccggccgcgc atcgcccggg tcgacgatcg cgacacggcg cgtgtcatgc agtgcccaga
      901 tgtagttgtc attgaacgcg ggaatgggaa cgacggtcgg tttggacatg atgcttcact
      961 ctgaattgaa aacgcggcgg aacgcggcac gggcgccggt cgtgcggccg atcgcgccgt
     1021 gtgcggcgct ggctaggccc tgattgtgtt gcactcgccg caaatgtcaa cgctgaccga
     1081 ctggttcggc acgcccaagg gcgcgtacgt gctcgaatgg gagctgacgc agttcgacaa
     1141 cgctgtggag gatgtgttcg gattcatcgc cgtgcaggtc ggctcgccga agctcgattt
     1201 cctgcgcgcc aaccgcgtgc cggtgcgcac ctgcatcggc ggtcccggtt gcggcgtgct
     1261 cggagagccg ttcgccttgc cgctcgcctc gcagagcgtc gacctcatcg tgctgccgca
     1321 tgtgctcgaa ttcagcgcga tgccccacca gatcctgcgc gaagccgagc gcgtattgcg
     1381 ccccgaaggc catatcgtca tctccggctt caatccgctc agcctgtggg gcgtgaagcg
     1441 gatgatctcg caccggcgcg gcgagtatcc atggtcgggc gatttcatcg gtctgctgcg
     1501 tctgcgcgac tggctgaagc tgctgggctt tgaactcaac ggcggccgct tcggctgtta
     1561 cgcgccgccg ttcagcacca ccaagtggct gcagcgcttt tccttcatgg aatcggcggg
     1621 cgatcgctgg tggcccatcg cgggcggcgt ctatgtggtg cgggcggtca agcgcgtgta
     1681 cggcatgcgt ctgttgcaac ccgattggaa gaatggcaag aagcggacga agaaagtcct
     1741 cgcgcccgtg gcccaacgcc acgacggcgt acaacggaac ggacatgaac gataagaaca
     1801 atgtcgtgcg cctttacacc gacggcgcat gcaagggcaa tccgggtgtg ggcggctggg
     1861 gcgtgctgat gcgctacggc gacaaggaaa aggaactgta cggcggcgag gccatgacca
     1921 ccaacaaccg catggagttg atggcggtga ttcgcgggct gaatgcgctc acgcggcctt
     1981 gcacggtcga ggtatggctc gattccgaat atgtgaagaa cggcatcacc cagtggatga
     2041 cgaactggaa gcggcgcggc tggaaaaccg ccgacaggaa gccggtgaag aacgccgagc
     2101 tatggcgcga actggacgag gcgacgcagc gtcacgagat cacctggcac tgggtgcgcg
     2161 ggcataccgg ccatgagggc aacgagcgtg cggatgcgct ggcgaatcgc ggcgtggagg
     2221 aggtgcgcgc gaacctggtg tccgcgcgct aaagccttaa aggcttaaac acagagaagc
     2281 gctttcaatc cttggctggt gcggattgcg cggtcggcgg atagtccgta tccgtccagc
     2341 cgccacccag ggcttccatc aggtcggtgg tgtaggacag ataggcctgc cggttgcgca
     2401 ccagttccag ttcggcctca ttcaaggtgc gctgcgcatc cagcacttcg aggaagctcg
     2461 aatacccgga ttcgtaacgc agccgcgcca gtcgcagcga attgcgtgcg gcttccacac
     2521 gcgcaagcag atccgcttcc atgtccgccg tccatgcaac gttgctgagt gcatccttca
     2581 cgtctcggaa ggcagcttcg gccgcctgct cgtagagtgc cgcggcctgt ttctgctgtg
     2641 cgacggcctg atcggtgcgc gcggcatagc gccccgcatc gaatatcggc cccatcaggt
     2701 tcaagccgac agaccagatc ttgttgccgg actcgaacag cgtggacagc gtgtcgcttt
     2761 gccggccgta tgagccggtc aggctgaaag tcgggaactg cgctgcgcgc gccacgccga
     2821 tggcggcatt ggcggcggcc agttgcgctt cggcttggcg cacgtcaggc cggcgctcca
     2881 gtagcgaggc gggtagcccc gcggggggaa cgggcggcga aggcaatgcg cgcacatcgc
     2941 ccggctccag ttgcagatcg aggatgccgg tcagctcacc cagttgatgc aaggtcacgt
     3001 cgcgctggcg ttgcacttcc ttcaattgcg cgcgcgcgcc tgcatgcgca ctttgcgcct
     3061 ggttcacgtc cagttcggaa ataaggccgg cctcgaaacg cttggtcgcg atatcgagcg
     3121 attcggccga tacgcgcacg ttttccgccg ccacttccgt ctgcgcctgc agcgaacgta
     3181 cggcgaagta cgtctgcgca atggccgatg cgagcgtgag cccgaccacg tcgcggccat
     3241 accgcgtcgc aagatattgc gcctgcgccg cttcgttggc gcgccgcaag cggccccaga
     3301 aatcgagttc gaaggcggtc gtcgcggtga gcgtaaaggt gttgcgcacc gtcgagacgc
     3361 cgggggtggc gggcgccatg ctgcgaccgc gcgaggcgcc gccgttcgcg tcgatggtgg
     3421 ggaagaacag cgaacgcgct tcgcgcagca ccgcctcggc ttcctcgatt cgcgcgacgg
     3481 caaacttcat atcggtgttg cgcgtcagac cggcttctac gagctcgttg agcttgggat
     3541 cgccgtacag cgtccaccaa tcggacggaa tgttcggttc gctgcgatcg gcgacggcgc
     3601 cgtagtccat cggcagatcg agggcggggc gctcatagtc gggacccacc gcgcaaccgg
     3661 cgagcgccag cagcgagacg gcgcagagca gtgtccgctt caggctcatg cgggttcctt
     3721 cagttcgtcg gacgcttggt ccggatgctt gtccgccttg cgcaggctcg cgaacagggt
     3781 gaagaacagc gggatgaaaa tcgtggcgat cgcggtggcc gccagcatgc cgccgaccac
     3841 gccggtgccc atggagcggc gcgctgccga gcctgcgccg ctggcgaccg ccagcggcag
     3901 cacgccgagg atgaaggcca gcgaagtcat cacgatcggg cggaaacgca ggcgcgccgc
     3961 ttcgagcgcc gcttccatcg gcttcttgcc tgcgagcaga ccctgctgcg cgaactcgac
     4021 aatcagaatc gcgttcttcg cggacaggcc gatcagcacc accagaccga tctggaagta
     4081 gatgtcgttc tccatgccgc gcagccacac caggccgagc gcgccgacga tggcgaaggg
     4141 cacggcgagc agcaccgcac tgggcagccg ccagcgttcg tacagtgcgg agaggatgag
     4201 gtacaccatc accagcgcga aaccgaaggc gaagatggac gcgctgccgg tgcctttctc
     4261 ctgatacgcc tggcctgtcc aggcaatggt gaaaccgggg ggcagcactt cggcggccac
     4321 ctgttcgact gccgcgatgg cggtgcccga actcacgccc ggcttgccgc tgcccatgac
     4381 cttggcgccg aggaagccgt tgaagcgctc gatctgctcg ggtccgacga tgggtttcac
     4441 ggtgaggaag gtcttcagcg gaatcatttc gccggtcgta ttcgagcgca catgaatgcg
     4501 gccaagatct tccggctgcg cacgataggg cgcgtcggcc tgcaactgca cg
//
LOCUS       DATGOU010000039        10646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_2310129, whole genome shotgun sequence.
ACCESSION   DATGOU010000039 DATGOU010000000
VERSION     DATGOU010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10646
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2310129"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VKZ48_02170"
     CDS             <1..989
                     /locus_tag="VKZ48_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HLU76692.1"
                     /translation="TFLILVFSEITPKVIGAAYPEKIALPLSYVLGPLLRLLYPVVWF
                     INLFSSALLWLMRINPQAGDAVQRLSNEELRSLVLEHSRYLPKKHSSILVNLFDLDRI
                     TVEDVMTPRAQIEGIDLEAPEEVLVEQLTTSYHTRLLAYRHEPGNVVGILHLRRALSI
                     MREDQLSKEALTEILHEPYYVPGDTGVLAQMQYFQENRQRMALVVDEYGELQGLITLE
                     DIVEEIIGEFTTSTPIRASSFDWGEGDSTLVEGASSLRELNRKLGLKLPLDGPKTLNG
                     LILDYLQDIPEAGVSVKISGVPMEIVQTQDRVIKTVRLFRPKVPVATEPETH"
     gene            1233..2471
                     /locus_tag="VKZ48_02175"
     CDS             1233..2471
                     /locus_tag="VKZ48_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HLU76693.1"
                     /translation="MATAAALKRGSRNDRVKEYVFLWEGKDRSGKQIKGEMRASGQNV
                     VRSTLRRQGLLVTKVKKQRMRSGKKITEKDVTLFTRQMATMMKAGVPLLQAFDIVGKG
                     HSNPSVAKLLFDIKTEVETGSSLAAAFRKFPLYFDALFCNLVQAGEQAGILETLLDRL
                     ATYKEKILAIKSKIKSALFYPIAVIVVAFIITAVIMIWVIPAFKEVFTSFGADLPAPT
                     LMVIAMSDFFVANWYLIFGGLIGGIWGFFELWKRSRAMQILLDRWFLRMPIFGSIIRK
                     AVIARWTRTLATMFAAGVPLVEALDSVGGASGNHVYGEATKQIQQEVSTGTSLTVSMQ
                     NAAVFPNMVIQMTAIGEESGSLDAMLSKVADFYEAEVDDAVAGISSLMEPLIMVVLGT
                     LIGGMVIAMYLPIFKLGQVV"
     gene            2532..3380
                     /locus_tag="VKZ48_02180"
     CDS             2532..3380
                     /locus_tag="VKZ48_02180"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HLU76694.1"
                     /translation="MPMPFDPLVYSIAALVVGLAVGSFLNVVIHRLPRMMEREWQAQC
                     AELRGEAPVAGERYNLALPRSACPHCGHRITALQNIPVLSYLLLRGRCAGCNARISVR
                     YPIIELLTGVATGYAAWRYGMTLAGFGAMVFLWAMIALTFIDADTQLLPDSITLPLLW
                     LGLLVNLNGTFVSLHAAVIGAVCGYLVLWSVYWLFKLATGKEGMGFGDFKLLAAIGAW
                     LGWQMLPLVILLSSLVGAVVGVVLIVLAKRGREVPMPFGPYLAAAGVIALFWGPGLVE
                     RYLDFL"
     gene            3380..3982
                     /gene="coaE"
                     /locus_tag="VKZ48_02185"
     CDS             3380..3982
                     /gene="coaE"
                     /locus_tag="VKZ48_02185"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734565.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HLU76695.1"
                     /translation="MYVVGLTGGIGSGKSSVANLFAERGIAVVDADEISHQLTAAGGG
                     AIARLRAEFGDEMIDAQGALDRARMRDLVFRDPDAKRRLEAVLHPLIREEAARQIADA
                     QSPYVIHMIPLLVEGGNARGRFDRVLVVDCPEETQIARVMARNGMTREQIEAIMATQA
                     SRAARLAEAHDIVDNSGSPAALPPQVQRLHKLYLRLAAQK"
     gene            4053..4808
                     /gene="zapD"
                     /locus_tag="VKZ48_02190"
     CDS             4053..4808
                     /gene="zapD"
                     /locus_tag="VKZ48_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HLU76696.1"
                     /translation="MITYEYPLIERIRTLLRLEDLFDRASHFLNKTDAPEHHVALLTL
                     FEILEVAGRADLKSELLQELERQKQMIGAFRNNPEVNQDALARILHDIESTYAALHAM
                     TGKVGQYLRENEWLMSVRQRTNIPGGACEFDLPSYHYWLNQPSEERLEALLSWISPLL
                     PIRDAAQMVLKLLRESGKPVVARAQGGTFQQMLSGRSAQMVRLRVPRTLLCVPEISAN
                     KYALNIRFTVVGEEARPRTCDTDIDFELTFCSL"
     gene            4808..4999
                     /gene="yacG"
                     /locus_tag="VKZ48_02195"
     CDS             4808..4999
                     /gene="yacG"
                     /locus_tag="VKZ48_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HLU76697.1"
                     /translation="MKSEPRQVPCPRCGTPVAWTSVNRYRPFCSERCKMIDLGAWANE
                     EYRLAAEEDDENPDDPRVS"
     gene            complement(4996..5952)
                     /locus_tag="VKZ48_02200"
     CDS             complement(4996..5952)
                     /locus_tag="VKZ48_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nudix family hydrolase"
                     /protein_id="HLU76698.1"
                     /translation="MSKVVVVAAAVLLRDDGSFLLAQRPSRTVYAGYWEFPGGKVETG
                     ETPEDALGREIREELGIDIERPEPWLTRTYTYPHATVRLHFFRVRSWQGEPHGREGQQ
                     LAWQRPEAPDVTPMLPANTPILLALTLPDEYAVSNAASVGIENFLQALEARLASGLRL
                     IQLRERDLHPDALEQLGREVMKRARAVNARVLLNGDVALAQRIGAHGVHLSARQLQAC
                     AARPDCELVGASVHTAEELRHAEALGLDFAVLGSVHATPTHPDTAPLGWAGFSDIARD
                     AALPVYAIGGMTREELAVARAHGAHGIAMIRGSWPSTAVPER"
     gene            complement(5952..6836)
                     /locus_tag="VKZ48_02205"
     CDS             complement(5952..6836)
                     /locus_tag="VKZ48_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLU76699.1"
                     /translation="MADALEKLIARLDGLVDRLEMLLPTPPAPVDWSASIAFRWRRRH
                     GRGQLVPVSHPHKIRLEDLQDVDRQKALVDANTRQFVRGLTANNVLLTGARGTGKSSL
                     VKALLNRYADEGLRLIEVDKLDLLDLPEIVELVSGRPERFIIFCDDLSFEANEPGYKA
                     LKVVLDGSIAAPSENVLVYATSNRRHLMPEYMHENLEYKHVGGEIHPGETSEEKISLS
                     ERFGLWVSFYPFDQEEYLRIVAHWLRTLGCDDAQIESARDEALQWALGRGSRSGRVAW
                     HFARDWAGRHRNGAAPDA"
     gene            complement(6840..8066)
                     /gene="argJ"
                     /locus_tag="VKZ48_02210"
     CDS             complement(6840..8066)
                     /gene="argJ"
                     /locus_tag="VKZ48_02210"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HLU76700.1"
                     /translation="MAVNLNPPDPAALKTVAGVELGYAEAGIRKQGRRDLMLMRFAPG
                     TQVAGVFTQNGFAAAPVQVCRERLAQHTEIRALLVNAGNANCGTGEQGLRAALATCEA
                     VAQQLGCTPAEVLPFSTGVILEDLPVDRIVAALPRCVASLAPANWAGAAQAIMTTDTV
                     PKAVSKTAEIGGRTVTVTGMAKGVGMLQPNMATMLAFVATDAKVEPPALRALVREVAD
                     ASFNRVTVDGDTSTNDSFVLIATGQSGTAAIGRGSPEYARLRDTAIAVAAELAQAIAR
                     DGEGATKFVTVQVNGARDDAEALRVARGIANSPLVKTALFASDPNLGRLLMAIGNADM
                     TDFDPARVDLYLGDVQVIDRGGRAASYREEDGVRVMKPAEITIRVELNRGGASTVVWT
                     CDFSYDYVKINAEYRT"
     gene            complement(8108..>10646)
                     /gene="secA"
                     /locus_tag="VKZ48_02215"
     CDS             complement(8108..>10646)
                     /gene="secA"
                     /locus_tag="VKZ48_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HLU76701.1"
                     /translation="VVREAGKRVLKMRHFDVQLIGGMVLHHGKIAEMRTGEGKTLVAT
                     LPAYLNALSCKGVHIVTVNDYLASRDAEWMGKLYRFLGLSVGVILSRMPHGDKHAAYA
                     ADITYGTNNEFGFDYLRDNMTMSVQERVQRKLNYAIVDEVDSILIDEARTPLIISGQA
                     EDRTEVYRRMDKVAPKLVRQETPESEGDFYVDEKQHTVLLSEAGHERAEALLEEAGLL
                     TGGSLYDAANINLMHHLNCALRAHNLFHKDRNYVVQNNEVVIVDEFTGRLMPGRRWSE
                     GLHQAIEAKEGVQIQSENQTLASITFQNYFRMYNKLAGMTGTADTEAYEFQEIYGLET
                     VVIPTHRPMVRDDRLDQVYKTFNEKFEAIKADIKDCYERGQPVLVGTTSIENSELLSR
                     LLDKEKLPHQVLNAKQHEREAQIVAQAGRPKMITIATNMAGRGTDIVLGGNVEKQIEF
                     IEADESLDSAEKERQIKQLRDEWQSLHDQVVAAGGLHIIGTERHESRRIDNQLRGRAG
                     RQGDPGSSRFYLSLEDQLLRIFAGDRIRAIMDRLKMPEGEPIEHPLVTRSIESAQRKV
                     EAHNFDIRKQLLEYDDVANDQRRNVYQLRNDILENDDLGDQVKALRDGVLTDLFRAYI
                     PEQSVEEQWELEGLENALRGELGLHVPVREWLEADGTLTDDDLLQRLIELGDQVYTER
                     MAIAPPEIVRQIERSVLLQTLDLNWREHLAALDQLRQGIHLRGYAQKNPKQEYKREAF
                     ELFGTFLETVKMETTRILLRLQLQTEEQIAQAEALERQAPQLENVQYQHPSFEEALVG
                     ADAEEAAVAASAPPKAAPFVRGEKIGRNEPCPCGSGKKYKHCHGKLA"
BASE COUNT         1896 a         3414 c         3287 g         2049 t
ORIGIN      
        1 tcaccttcct cattctcgtc ttctccgaaa tcacgcccaa agtcatcggt gcggcgtatc
       61 cggaaaaaat cgccctgccg ctgtcctatg tactaggccc cctgctgcgg ctgctctacc
      121 cggtcgtctg gttcatcaac ctgttttcgt ccgcgctgct gtggctgatg cggatcaacc
      181 cgcaggccgg cgacgcggtg caacggctca gcaacgagga attgcgttcg ctggtgctgg
      241 aacacagccg ctatctgccg aagaagcatt cctccatcct ggtcaacctg ttcgatctgg
      301 atcgcatcac ggtggaggat gtgatgacac cgcgtgcgca gatcgaaggg atcgacctgg
      361 aagcgcccga ggaggtgctc gtcgaacagt tgaccacgag ctatcacacc cggctgctgg
      421 cttatcggca cgagcccggt aacgtggtcg ggattctgca cctgcgtcgc gcattgtcga
      481 tcatgcgcga ggatcaactg tccaaggaag cgctgaccga aattcttcac gaaccgtatt
      541 acgtgcccgg cgataccggc gtacttgcgc aaatgcagta tttccaggag aaccggcagc
      601 gcatggcact ggtggtcgac gagtacggcg aattgcaggg cctgatcacg ctcgaggaca
      661 tcgtcgagga aatcatcggc gagttcacca ccagcacgcc catccgcgcg tcgagcttcg
      721 actggggcga gggcgacagc acgctggtcg aaggggccag cagtctgcgc gaactcaatc
      781 gtaagctggg gctgaaactg ccgctcgatg gacccaaaac gctcaacggt cttattctcg
      841 attatctgca ggacattccg gaggccggcg tcagcgtaaa aatcagcggc gtgccgatgg
      901 aaatcgtgca aacgcaggat cgcgtcatca agaccgtccg cctgttccgc cccaaggtac
      961 ctgtcgccac cgagcccgag acgcactgat ttgccattgt catacgccga gtgtgacgat
     1021 gttccccctt gggagcccga agctcacccc caccgacgcc gatccggcga aaatgtccac
     1081 gatacccgct caagcgtgac gggcatcacg tgcgcggcca gcccgggcag ggcagcggac
     1141 gcgaagcacg cgagaatcgt gcgaccggcg agcggccggc ttccctgcct gactcaccgg
     1201 acagcaatcg gatttaagaa cgaggcgcag atatggcgac cgcagcagca ctcaagcgtg
     1261 gctccagaaa cgaccgtgtg aaggaatacg tcttcctgtg ggaaggcaag gaccgcagcg
     1321 gcaaacagat caagggcgaa atgcgggcga gcggccagaa cgtggtccgc tccaccttac
     1381 gtcgccaggg cctgctggtc accaaggtga agaagcagcg catgcgctcg ggcaagaaaa
     1441 tcaccgagaa ggacgtgacg ctgttcaccc gccagatggc gaccatgatg aaagccggcg
     1501 tgccgcttct gcaggccttc gacatcgtcg gtaagggaca ctccaacccc agcgtcgcca
     1561 aactgctgtt cgacatcaag accgaagtgg aaaccgggtc cagcctcgcc gcagccttcc
     1621 gcaagtttcc gctctatttc gatgcgctgt tctgcaacct ggtacaggca ggcgaacagg
     1681 ccggtattct ggaaacgctg ctcgatcgcc tggcgacgta caaggaaaag attctcgcca
     1741 tcaagagcaa gatcaagtcg gcgctgttct atcccattgc cgtcatcgtg gtggcgttca
     1801 tcattacggc ggtcatcatg atctgggtga tcccggcttt caaagaggtg ttcaccagtt
     1861 tcggcgctga cctgcctgca ccgacgctga tggtgatcgc catgtccgac ttcttcgtcg
     1921 ccaactggta tctgatattc ggcggactga tcggcggcat ctggggtttc ttcgaactgt
     1981 ggaagcgttc ccgcgccatg cagatcctcc tcgaccgctg gttcctgcgc atgcccatat
     2041 tcggctccat cattcgcaag gcggtgatcg cacgctggac gcgcaccctg gcgacgatgt
     2101 tcgccgcggg cgtgccgctg gtggaagcgc tggattcggt gggcggtgcc tccggcaacc
     2161 acgtctatgg ggaggcgacc aagcagattc agcaggaggt cagcaccggt acgagcctta
     2221 ccgtgtcgat gcagaacgct gccgtgttcc ccaacatggt cattcaaatg accgccatcg
     2281 gcgaagagtc cggctcgctg gacgccatgc tgagcaaggt ggccgacttc tacgaagcgg
     2341 aagtggacga cgccgtcgcc ggcatttcca gcctgatgga gccgctcatc atggtcgtgc
     2401 tcggcacgct tatcggcggc atggtgatcg cgatgtatct gcccatcttc aagttgggcc
     2461 aggtcgtctg aggccgctgc ccatcgcaag ggctgcccac gaggcagccc ttttttgtta
     2521 caacgcctcc tatgcccatg cctttcgatc cgctcgtcta cagtattgcg gcgctcgtcg
     2581 tcggacttgc cgtcggcagc tttctgaatg tggtgatcca tcgcctgccg cgcatgatgg
     2641 agcgcgaatg gcaggcgcaa tgcgcggaac tgcgtgggga agcgcccgtc gcgggcgagc
     2701 gctataacct tgcgctgccg cgctcggcct gcccgcattg cggccatcgc atcaccgcgc
     2761 tacagaacat ccccgtcctc tcgtacctgc tgctgcgcgg caggtgcgcc ggttgcaatg
     2821 cacgcatcag tgtccgctat cccatcatcg aattgcttac gggtgtggcg acaggctacg
     2881 ccgcctggcg ttacggcatg acgctcgcgg gtttcggcgc gatggttttc ctgtgggcaa
     2941 tgattgcgct gacattcatc gatgcggaca cccaactgct gcccgactcg atcacgcttc
     3001 cgctgctctg gctcggcctg ctcgtcaatc tgaacggcac cttcgtcagc ctgcatgccg
     3061 ccgtgatcgg tgcagtatgc ggctatctgg tgctgtggtc cgtgtactgg ctgttcaagc
     3121 tggcgaccgg caaggagggc atgggcttcg gcgatttcaa actgctcgcc gccatcggcg
     3181 cctggctggg atggcagatg ctccccctcg tcattctgct gtcctcactg gtaggcgcag
     3241 tggtgggggt ggtcctcatc gtgctggcca aacgcggccg tgaagtgccc atgcctttcg
     3301 gcccctatct cgccgccgcc ggcgtgatcg ccctgttctg ggggccgggg ttggtcgagc
     3361 gttatctgga ttttctctga tgtacgtcgt cggattgacc ggaggcatcg gcagcggcaa
     3421 atcgagcgtc gccaatctgt tcgcggaacg aggaatcgcc gttgtcgatg cggatgaaat
     3481 ttctcatcaa ctcaccgcgg cgggcggcgg cgcgatcgca aggctgcgtg cggaattcgg
     3541 cgacgagatg atcgatgccc agggcgcact cgaccgcgca cgcatgcgcg accttgtatt
     3601 ccgcgatccg gatgcaaagc ggcgattgga ggcggtgctg cacccgctca tccgcgagga
     3661 agcggcacgc cagatcgccg acgcgcaaag tccgtacgtc atccacatga tcccgctgct
     3721 ggtggaaggc ggcaacgcgc gcgggcgctt cgatcgtgtg ctggtcgtgg actgtcccga
     3781 agagacgcag attgcgcgag tcatggcgcg caacggcatg acgcgggaac agattgaagc
     3841 gatcatggcg acccaggcat cgcgcgccgc acggttggcg gaggcgcacg atatcgtgga
     3901 caactccgga tcgcccgccg cattgccgcc gcaagtgcag cgtttgcaca agctttatct
     3961 gcgtctcgcc gcgcagaagt gatgctgcta cgcggttgtc atgcttcggt gattggttca
     4021 gaatcggccc gaccttctct cacggaccgg gcgtgatcac ttacgagtac ccactgatcg
     4081 aacgcatccg gacgttactc cgtctggaag atctattcga tcgtgccagc cactttttga
     4141 ataagacgga tgcgccggag catcatgtcg cgctgctgac gctgttcgag attctcgaag
     4201 tcgccggacg cgccgacctg aaatccgaac tgctgcagga gctcgagcgc cagaagcaga
     4261 tgatcggcgc cttccgcaac aatccggaag tgaatcagga cgcgctcgcc cgcatcctcc
     4321 acgacatcga gtcgacttac gccgcactgc acgccatgac cggcaaggtc ggtcagtatc
     4381 tgcgcgaaaa cgagtggctg atgagcgtgc gccagcgcac caatattccg ggcggcgcct
     4441 gcgagttcga tctgccgtcg tatcactact ggctcaatca gccgagcgag gagcgtctgg
     4501 aggccctgct gtcgtggatc tcgccgctgc tcccgattcg cgatgctgcg cagatggtgc
     4561 tcaagctgct gcgtgagagc ggcaaacccg tcgtcgcacg ggcacagggc ggcacgttcc
     4621 agcagatgct gagcggccgc tcggcgcaga tggtgcgtct gcgcgtgccg cgcacgctgc
     4681 tgtgcgttcc ggaaatcagc gccaacaaat atgcgctgaa tatccgcttc accgtggtgg
     4741 gtgaagaggc gcgcccgcgt acttgcgaca cggacatcga cttcgaattg actttctgca
     4801 gcctgtaatg aaatcagaac cgcgtcaggt cccctgcccg cgctgcggca cgccggttgc
     4861 ctggaccagt gtcaaccgct atcgcccgtt ctgctccgag cgttgcaaga tgatcgatct
     4921 gggcgcttgg gcgaacgagg aataccggct cgcagcggaa gaggacgacg agaatcccga
     4981 cgacccgcgg gtgtcctagc gttccggcac agcggttgac ggccaactgc cgcgaatcat
     5041 cgcaataccg tgtgccccgt gcgcacgggc gacagccagt tcctcgcgtg tcatgccgcc
     5101 gatcgcataa acgggcagcg ccgcgtcgcg ggcgatgtcc gagaacccgg cccagccgag
     5161 cggtgcggta tccggatgcg tcggcgtggc atgcaccgaa ccgagcacgg cgaaatccaa
     5221 tccgagcgct tccgcatggc gcaactcttc agcggtatgt accgaagcgc cgaccagttc
     5281 gcaatccgga cgcgcggcgc atgcctgcaa ctgccgcgcg ctgagatgca cgccgtgcgc
     5341 accgatacgc tgcgccaggg cgacatcgcc attcaggagt acgcgcgcat tgacggctcg
     5401 tgcgcgcttc atcacttcac gcccgagttg ctccagggca tccggatgca gatcgcgctc
     5461 gcgcaactgg atcaggcgca accccgaagc gagtcgcgct tccagcgcct gcaaaaaatt
     5521 ctcgatgccc acagacgcgg cgttcgatac ggcgtattcg tccgggagtg tcaacgcaag
     5581 cagaatcggc gtattggccg gcagcatggg cgtgacgtcc ggtgcttccg gtcgctgcca
     5641 ggccagttgt tgaccttctc gcccatgcgg ctcaccctgc cacgaacgca cacgaaagaa
     5701 atgcaggcgg accgtcgcgt gcgggtaggt ataggtccgt gtcagccagg gctcgggccg
     5761 ttcgatgtcg atgccgagct cttcgcggat ttcacgcccg agtgcatcct ccggcgtttc
     5821 gcccgtctcg accttgccgc ccggaaattc ccaatagccg gcatacacgg tgcgcgaagg
     5881 acgctgtgca agcagaaaac tgccgtcatc ccgcagcagg accgccgccg cgacgaccac
     5941 tactttgctc atcaggcgtc cggcgcggcg ccgtttcgat ggcgccccgc ccagtcgcga
     6001 gcgaagtgcc aggcgacccg gccgctgcgc gaaccgcgtc cgagcgccca ttgcaacgcc
     6061 tcgtcgcgcg cgctttcaat ctgcgcatcg tcgcaaccga gggtgcgcaa ccagtgcgcg
     6121 acgatgcgca gatactcttc ctgatcgaag ggatagaagg acacccataa gccgaagcgt
     6181 tcggacagcg agatcttctc ctcgctagtt tcgcccggat ggatttcgcc gcctacatgc
     6241 ttgtactcga gattctcgtg catgtactcg ggcatcaggt gccggcggtt ggaggtggcg
     6301 tagaccagca cgttttccga aggcgcggcg atggaaccgt ccagcaccac cttgagcgcc
     6361 ttgtagccgg gctcgttcgc ctcgaaggac aggtcgtcgc agaagatgat gaagcgctcc
     6421 ggacgcccgc taaccagttc gacgatctcg ggcaggtcga gcagatcgag cttgtccact
     6481 tcgatgagac gcagaccttc gtccgcatag cgattcaaca gggctttgac cagcgaactc
     6541 ttgccggtgc cgcgcgcgcc cgtcagcagc acattgttcg cggtcaggcc gcggacgaac
     6601 tggcgcgtat tggcatcgac cagggctttc tgccgatcga cgtcctgcag atcctcgagc
     6661 cggattttgt gcggatgaga gaccggcacg agttggccgc ggccatgacg gcggcgccag
     6721 cgaaaagcga tgctggccga ccagtcgacc ggagcgggcg gtgtgggcag cagcatttcc
     6781 agccggtcca ccagaccgtc cagacgcgcg atcagttttt ccagtgcgtc agccatcgct
     6841 caggtgcggt actccgcatt gatcttgacg tagtcgtagg agaaatcgca tgtccacacc
     6901 acggtggacg caccgccgcg attgagttcg acgcgtatgg tgatctcggc gggcttcatc
     6961 acgcgcacac catcttcctc gcggtagctc gcggcgcgcc cgccccggtc gatcacctgc
     7021 acgtccccca ggtacagatc gacgcgagcc ggatcgaaat cggtcatatc cgcgttgccg
     7081 atcgccatga gcagccggcc cagattggga tcggaagcga atagcgccgt cttcaccagc
     7141 ggcgagttcg cgatgccgcg tgccacccgc agtgcttcgg catcgtcgcg cgcgccgttc
     7201 acctgcacgg tgacgaattt ggtcgcgccc tcgccgtcgc gcgcgatcgc ctgtgcaagc
     7261 tctgccgcca cggcgatcgc cgtatcgcgc agccgcgcat actccggcga gcccctgccg
     7321 atcgctgcgg taccgctctg gcccgtggcg atcagaacaa aggaatcgtt ggtggacgtg
     7381 tcgccgtcca cggtgacgcg gttgaacgag gcgtcggcca cctcacgcac cagcgcgcgc
     7441 aaggccggcg gctccacctt ggcatcggtc gcaacgaagg cgagcatggt ggccatgttc
     7501 ggctgcagca tgcccacgcc cttggccatg ccggtcacag tgacggtgcg gccgccgatc
     7561 tccgccgtct tgctgacagc cttcggcacc gtatcggtgg tcatgatggc ctgcgccgca
     7621 ccggcccagt tggccggtgc aaggctcgca acacaacgcg gcaatgccgc gacgatgcga
     7681 tccaccggca ggtcctcgag aatgacgccc gtcgaaaaag gcagcacctc cgccggcgtg
     7741 cagcccaact gctgcgcgac cgcttcgcag gtcgcaagcg ccgcgcgcaa tccctgttca
     7801 ccggtaccgc agtttgcgtt gccggcattg acgagcaagg cacgaatttc ggtgtgctgc
     7861 gccagccgct cgcgacacac ctgcacaggc gccgcggcaa aaccgttctg cgtaaacacg
     7921 ccggccacct gcgtgcccgg tgcaaagcgc atcagcatca ggtcgcgacg accctgcttg
     7981 cggatgccgg cttcggcgta gcccagctcg acgcccgcca cagtcttcaa tgcggcggga
     8041 tccggcggat tgagattgac ggccattagg aaacctactt tgaaccgacc ataatcgcgg
     8101 cgcgcggtta agcgagcttg ccgtgacagt gcttgtattt cttgccggaa ccgcaggggc
     8161 agggctcgtt gcgcccgatc ttctcgccgc gcacgaaagg cgccgccttg gggggcgcgc
     8221 ttgccgcgac ggcggcttcc tcggcatcgg cgccgaccaa cgcttcctcg aagctcggat
     8281 gctggtattg gacgttttcc agctgcggtg cctgccgctc gagcgcctcg gcttgcgcaa
     8341 tctgctcctc ggtctgcaac tgcagccgca gcaggatgcg cgtcgtttcc attttgacgg
     8401 tttcgaggaa ggtgccgaac agctcgaacg cctcgcgctt gtattcctgc ttcgggttct
     8461 tctgcgcata gccgcgcagg tggatgccct ggcgcagctg atccagcgcg gccaggtgct
     8521 cgcgccaatt gagatcgagc gtctgcagca gcacgctgcg ctcgatctga cggacgatct
     8581 ccggcggcgc gatggccatg cgctcggtat agacctggtc gcccagctcg atgagacgct
     8641 gcaacagatc gtcgtcggtc agcgtgccgt ccgcctcgag ccattcccgc accggcacat
     8701 gcagccccaa ctcgccgcgc agcgcattct ccaggccctc gagctcccac tgctcttcga
     8761 cgctttgctc aggtatataa gcacggaaca gatcggtgag cacgccgtcg cgcagcgcct
     8821 tcacctgatc gccgaggtcg tcgttctcga ggatgtcgtt gcgcaactgg tatacgttcc
     8881 ggcgctgatc gttggcgacg tcgtcgtact ccagcagttg tttgcggatg tcgaagttgt
     8941 gcgcctcgac cttgcgctgc gccgactcga tggaacgcgt caccagcgga tgctcgatgg
     9001 gctcgccctc gggcatcttc agccggtcca tgatggcgcg aatccggtcg ccggcaaaaa
     9061 tgcgcaggag ctggtcttcc agcgaaagat agaagcgcga agatcccggg tcgccctggc
     9121 gccccgcacg accacgcaac tggttgtcga tccgtcgcga ttcgtgacgt tcggtaccga
     9181 tgatgtgcag accgcccgcg gcgacgacct gatcgtgcag cgactgccac tcgtcgcgca
     9241 gttgcttgat ctgccgttcc ttctccgcgg aatcgaggct ctcgtcggct tcgatgaatt
     9301 cgatctgctt ttccacgttg ccgcccagca cgatgtcggt gccgcggccc gccatgttgg
     9361 tggcaatggt gatcatcttc ggccgccccg cctgggcgac gatctgcgct tcgcgctcgt
     9421 gctgcttggc gttcagcacc tggtgcggca gtttctcctt gtcgagcaga cgcgacagca
     9481 attcggagtt ttcgatggag gtcgtgccga ccagcaccgg ctggccgcgt tcgtagcagt
     9541 ccttgatgtc ggctttgatc gcctcgaatt tttcgttgaa ggtcttgtag acctgatcca
     9601 gacggtcgtc gcgaaccatc ggccggtggg tggggatcac caccgtctcg agtccgtaga
     9661 tctcctggaa ttcgtacgct tcggtatccg ccgtaccggt cataccggcc agcttgttgt
     9721 acatgcggaa atagttctgg aaggtgatcg aggcgagcgt ctggttctcg ctctggatct
     9781 gcaccccctc cttcgcctcg atggcctgat gaaggccttc ggaccagcgg cggcccggca
     9841 tgagacggcc ggtgaactcg tcgacgatca ccacctcgtt gttctggacg acgtagttgc
     9901 ggtccttgtg gaacaggttg tgcgcgcgca gcgcgcagtt gaggtgatgc atcagattga
     9961 tgttcgcggc atcgtaaagg ctgccgccgg tgagcaggcc ggcctcctcc agcagtgcct
    10021 ccgcacgctc gtgccccgcc tcggacagca gcacggtgtg ctgcttttcg tcgacgtaga
    10081 aatctccctc ggactccggc gtttcctgcc gcacgagctt gggtgccacc ttgtccatgc
    10141 gacggtagac ttccgtgcgg tcctccgcct gaccggagat gatcagcggc gtgcgcgcct
    10201 cgtcgatcag gatcgagtcg acctcgtcga cgatggcgta gttcagctta cgctggacgc
    10261 gctcctgcac gctcatcgtc atgttgtcgc gcaaatagtc gaagccgaat tcgttgttgg
    10321 tgccgtaggt gatgtcggcg gcgtacgcgg cgtgtttatc cccatgcggc atccgcgaca
    10381 ggatcacgcc caccgacagt ccgagaaagc ggtaaagctt gcccatccat tcggcatcgc
    10441 ggctggcgag ataatcgttg acggtgacga tatgcacgcc cttgcaggac agcgcattca
    10501 gataggcagg cagcgtcgcg accagcgtct tgccttcgcc cgtgcgcatc tcggcgatct
    10561 tgccgtggtg cagcaccatg ccgccgatca actgcacatc gaagtggcgc atcttcagga
    10621 cgcgcttgcc cgcctcgcgc accacc
//
LOCUS       DATGOU010000040         4689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3209353, whole genome shotgun sequence.
ACCESSION   DATGOU010000040 DATGOU010000000
VERSION     DATGOU010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3209353"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VKZ48_02220"
     CDS             <1..598
                     /locus_tag="VKZ48_02220"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLU76702.1"
                     /translation="HRVALKKSAPSGPSPAEQGYAAYSRGDLDAARTHYETALERDPF
                     NRDALLGLAALDTRQQDFLSAEARYLRLLEMDPRDPYAHAGLLSLRGQQDAVAAESRI
                     KNLLSQRPDANVLHFTLGNQYAAQGRWPEAQQAYFKAYASEPTNPDYAFNLAVSLDHL
                     RQPRLALEYYQKALKLAAERPASFQAAQAESRIVELDR"
     gene            615..2318
                     /locus_tag="VKZ48_02225"
     CDS             615..2318
                     /locus_tag="VKZ48_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HLU76703.1"
                     /translation="MKAPVQAKRHIGQILIAQGILTEDQLRIALLEQLKSRQPVGKLL
                     VQLGFVSEATLRDALSEKLGLKSVDLSQIVVDPHAIRLVPRDFAKRYNVFPVALDRER
                     NALILAISDTNNIVALDQVRAQLRGQHDLDLRLSGESEIARAIDYFYGHELSIDGILH
                     EIETGEIDYQSLQASGDEYSQPVVRLISALLADAVERGASDIHFEPEASFLRIRYRID
                     GVLRQIRSLHKTYWPAMAVRIKVMAKMNIAETRAPQDGRISLSLTGRQVDFRVASQIT
                     THGENIVLRILDRQKGIVPLEGLGLQDEHLTMLKRMIARPEGIILVTGPTGSGKTTTL
                     YSILNHINHDGVNIMTLEDPVEYPMTLIRQTSVNEAAKLDFANGIRSLMRQDPDIILV
                     GEIRDEDTAQMAFRASMTGHQVYSTLHSNSAIGAIPRLLDTGLSPEIMAGNIIGIIAQ
                     RLVRRLCKHCRQPHEPNEAERRLLGNRSGETPRIYRAVGCDHCDYQGYRGRLAIMELL
                     KIDPEIDEMMANRATARELLRRALAKGFRTLADDGVRRVLDGSTSLEELMRVVDLTDR
                     M"
     gene            2461..3660
                     /locus_tag="VKZ48_02230"
     CDS             2461..3660
                     /locus_tag="VKZ48_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HLU76704.1"
                     /translation="MALYSYKAIDATGKTVLGQIDALNIVDLELRLKRMGLDLVVGGP
                     AKGRAGIGRGVKRVDLINFCFQLEQLTVSGVPMIEGLTDLRDSVENVRFREVVSGLIE
                     SIQGGQTLSGAMADYPLVFTPVLRSLIKAGEDTGRLPEVLRSLTENLKWEDELAAQTK
                     RIMIYPTIVGTLVIAVTFFLMIYLVPQMMAFIKTMGQTIPLHTRILIAVSDFFVHYWW
                     LLIFLPIGGFFLLKWLVAHNPLVRYRIDAMKLRMPVIGPILEKIILSRFASVFALMYS
                     SGITITESIRAAEESAGNAVIQDGLQRVGQQIADGKNVTAAFQDVGLFPPLVIRMVSV
                     GESTGMLDRALLNVGYFYNREVREAIPKAQAMIEPAMTIVLGALMLWVMLSVLGPIYD
                     TISKIKI"
     gene            3670..>4689
                     /locus_tag="VKZ48_02235"
     CDS             3670..>4689
                     /locus_tag="VKZ48_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76705.1"
                     /translation="MARTRLLYYSPAGVTLYQWRAGVLSEEARFELSDAGLARFADHC
                     AVLRSGIVHVLLDVVEEAFHIDTIPFVRGADRRALLQRRLAQRYRDTALSTAISLGYE
                     RSMRREERVLLSAFTNAADIQPWLQALEHEEVPVSGVYSPAVLAHQLARKLKFSKPSM
                     LLVGFHAGGVRQTYLRRGRAEFSRLNPLAAEDIGDGAAITAALERETDRLFKYLRNIH
                     THLEGEQTDAIVITPPGQTQRMHGFSTDTSVQLRMQLIDQHTAAKRIGLKQAPPNAGI
                     EALFLHMLALRRPSHQYAPAAIRASYGVRAWQIGLTAGGAAICAACLFAAGVQFLQTW
                     QMEGEI"
BASE COUNT          901 a         1518 c         1429 g          841 t
ORIGIN      
        1 gcaccgcgtc gcactgaaga agtccgcgcc gagcggcccc tcccctgcgg agcagggtta
       61 tgctgcgtat tcgcgaggcg acctcgatgc cgcgcgcacg cattacgaaa cggcgctgga
      121 gcgcgatccg ttcaatcgcg acgcgctgct cggcctggcc gcgctcgata cccggcagca
      181 ggattttctt agtgcggagg cacgctatct gcgcctgctg gaaatggacc cgcgcgatcc
      241 ttacgcccat gccgggctgc tgagtctgcg cggccagcag gatgcggtgg ccgcggaaag
      301 ccggatcaag aacctgctct cgcagcgccc ggatgccaac gtgctgcact tcacgctcgg
      361 caaccagtac gccgcacaag gccgctggcc cgaggcgcag caggcttact tcaaagctta
      421 cgccagcgaa ccgaccaacc cggattacgc tttcaacctc gcagtgagcc tggaccactt
      481 gcgtcagccg cggctcgccc tggagtatta ccagaaggct ttgaagcttg cggccgaacg
      541 tcccgccagc tttcaggccg cacaggcaga aagccgcatt gtcgaactcg atcgctgatc
      601 cccttgatgt attgatgaaa gccccggtgc aagccaagcg tcatatcggt cagattctga
      661 ttgcccaagg catcctgacg gaagaccagt tgcgcatcgc gctgctggaa cagctcaaat
      721 cccgccagcc ggtcggcaag ctgctggtgc agctaggttt cgtttcagaa gcgacgctgc
      781 gcgacgcgct gtccgaaaaa ctcggactga agagcgtcga tctgtcgcag atcgtcgtcg
      841 atccccacgc catacggctg gtgccgcgcg acttcgccaa gcgctacaac gtctttcccg
      901 tggcgctcga tcgcgagcgc aatgcgctga tcctggccat ttcggacacc aacaacatcg
      961 ttgcgctgga ccaggtgcgc gcgcagttgc gcggccagca cgacctcgac ctgcggctgt
     1021 cgggcgaatc ggaaatcgcc cgcgccatcg attacttcta cggccacgag ctgtcgatcg
     1081 acggcatcct gcacgagatc gagacgggcg agatcgacta ccagagcctg caggcttcgg
     1141 gcgacgaata cagtcagccg gtcgtgcgac tgatctcggc actgctcgcc gacgcagtgg
     1201 agcgcggcgc ctcggacatc cacttcgagc ccgaagccag ttttctgcgc atccgctatc
     1261 gcatcgacgg tgtgctgcgg caaatccgct cgctgcacaa gacttactgg ccggcgatgg
     1321 ccgtgcgcat caaggtcatg gcgaagatga acatcgccga aacgcgcgca ccgcaggacg
     1381 gacgcatttc gctctcgctc accgggcgac aggtggactt ccgcgtcgct tcgcagatta
     1441 cgacgcacgg cgagaacatc gtgctgcgca tcctcgaccg tcaaaaaggc atcgtgccgc
     1501 tggaaggtct ggggctgcag gacgaacacc tcaccatgct gaaacgcatg atcgcgcggc
     1561 ccgaaggcat catcctggtg accggaccga cgggcagcgg caagacgacg acgctttatt
     1621 cgatcctcaa tcacatcaat cacgacgggg tcaacatcat gaccctcgag gacccggtcg
     1681 aatatccgat gacactgatt cgccagacgt cggtcaacga ggcggcgaag ctggatttcg
     1741 ccaacggcat ccgctcgttg atgcgacagg acccggacat catcctggtc ggcgagattc
     1801 gcgacgaaga cacggcacag atggcgttcc gcgcctcgat gaccggccac caggtatatt
     1861 cgacgctgca ctccaattcg gccatcggcg ccattccgcg cctgctcgac acgggcctgt
     1921 cgccggaaat catggcgggc aacatcatcg gcatcattgc ccagcgactg gtgcggcgcc
     1981 tctgcaagca ctgccgccag ccccatgagc cgaacgaagc cgagcgccgg ctgctgggca
     2041 accgctcggg cgagacgcct cgcatctatc gcgcggtcgg ttgcgatcat tgcgactacc
     2101 agggctaccg tggccggctg gcgatcatgg aactgctcaa aatcgatccg gaaatcgacg
     2161 aaatgatggc caaccgcgcc accgcgcggg aactgctgcg gcgagcgttg gccaagggct
     2221 tccgtacgct ggcggacgac ggcgtacgcc gcgtgctgga cggttcgact tcgctggaag
     2281 aactgatgcg ggtggtggac ctgaccgacc gcatgtaacg gccggtttcc gcggtctgca
     2341 ggaggaatgt gtcacgcggt cgacggtaaa tggtgaaggg catcacaagc gcctcgcgat
     2401 taaagtttcc cccttttcat caatgcatta acgtaagcat ccgcaatccg gccgtccccc
     2461 atggcgctct acagttacaa ggcgatcgat gccaccggta aaacggtgct cggccagatc
     2521 gacgcactga acatcgtcga tctggagttg cgtctcaagc gcatgggact ggacctggtc
     2581 gtcggcgggc cggccaaggg ccgcgccggc atcggccgcg gcgtcaagcg cgtcgatctc
     2641 atcaacttct gcttccaact cgaacagctc acggtttcgg gcgtgccgat gatcgagggc
     2701 ctcaccgatc tgcgcgacag cgtcgagaac gtgcgcttcc gcgaagtcgt gtccggattg
     2761 atcgaatcca ttcagggcgg acaaacgctt tcaggcgcga tggccgacta tccgttggta
     2821 ttcacgccgg tactgcgttc gctgatcaag gcgggcgaag acaccggccg gctgccggaa
     2881 gtgctgcgca gcctcaccga aaacctgaag tgggaagacg aactcgccgc gcaaaccaag
     2941 cggatcatga tctacccgac catcgtgggt acgctggtga tcgcggtgac cttcttcctg
     3001 atgatctacc tcgtgccgca gatgatggct ttcatcaaaa cgatggggca aaccatcccg
     3061 ctgcacacgc gcatcctgat cgcagtttcc gacttcttcg tgcactactg gtggctgctc
     3121 atctttctcc cgatcggcgg gttcttcctg ctgaagtggc tggtggcgca caacccgctg
     3181 gtccgctacc gcatcgacgc gatgaaactg cgcatgccgg tgatcgggcc gatcctggaa
     3241 aagatcatcc tgtcgcgctt cgccagcgtg ttcgcgttga tgtattcgtc gggcatcacc
     3301 atcaccgagt cgatccgcgc ggctgaggag tccgccggta acgccgtcat tcaggacggg
     3361 ctccagcgcg tcggccagca gattgccgac ggcaagaacg tgacggcggc gttccaggac
     3421 gtcggcctgt ttccgccgct ggtcatccgc atggtgagcg tgggcgagag caccggcatg
     3481 ctggatcgcg cgctgctcaa cgtgggttac ttctacaacc gcgaagtgcg tgaagcgatt
     3541 cccaaggcac aggcgatgat cgagccggcc atgacgatcg tgctcggcgc gctgatgctg
     3601 tgggtcatgc tgtcggtcct cgggccgatc tacgacacca tcagcaagat caagatctag
     3661 gtgcaggcga tggcgcgcac gcgactcctc tactattccc cggccggcgt cacgctgtat
     3721 caatggcgtg caggcgtact gagcgaagag gcgcgcttcg aactgtccga cgccggcctc
     3781 gcccgcttcg ccgatcactg cgctgttctt cgatcgggca tcgtgcacgt gctgctggac
     3841 gtcgtcgaag aagcctttca tatcgacacc atccccttcg tgcgcggggc cgatcgtcgt
     3901 gcgctcctgc aacgtcgcct cgcgcagcgt tatcgcgaca ccgcgctgag cacagccatt
     3961 tctctcggct acgaacgcag catgcgccgg gaagagcgcg tgctgctcag cgcattcacc
     4021 aatgcggccg acatccagcc ctggctgcaa gcgctggagc atgaagaggt gccggtttcc
     4081 ggcgtctatt cgcccgcagt gctggcccat caactggccc gcaaactgaa attctccaaa
     4141 ccgagcatgc tactggtggg ctttcatgcg ggcggcgtgc ggcagactta tctgcgacgc
     4201 ggacgtgcgg aattcagccg gctgaatccg ctggcggccg aggatatcgg cgacggggcg
     4261 gcaatcacgg cggcgctgga acgggaaacc gaccggctgt tcaaatacct gcgcaacatt
     4321 cacacgcacc tggaaggcga gcagaccgac gcgatcgtca tcaccccgcc cggccagacg
     4381 cagcggatgc acgggttcag tacggatacg tcggtgcagt tgcgcatgca actcatcgat
     4441 cagcacaccg ctgccaagcg catcggcctc aaacaggcgc cgcccaatgc gggcatcgaa
     4501 gcgctatttc tgcacatgct cgccttgcgg cgtccgtcgc accaatacgc gcccgccgcg
     4561 atccgtgcga gctatggcgt acgcgcctgg cagatcgggc tcacggcagg cggggctgct
     4621 atctgcgcgg cctgcctgtt cgccgccggc gtgcaattcc tccagacttg gcaaatggag
     4681 ggcgagatc
//
LOCUS       DATGOU010000041         6764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_990007, whole genome shotgun sequence.
ACCESSION   DATGOU010000041 DATGOU010000000
VERSION     DATGOU010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990007"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..239)
                     /gene="rpoZ"
                     /locus_tag="VKZ48_02240"
     CDS             complement(30..239)
                     /gene="rpoZ"
                     /locus_tag="VKZ48_02240"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HLU76706.1"
                     /translation="MARITVDDCLEKIPNRFQLTLAATYRARQLAQGGTPTVEPDRDK
                     PTVIALREVAAGNVGLEMLSRVHQS"
     gene            complement(242..859)
                     /gene="gmk"
                     /locus_tag="VKZ48_02245"
     CDS             complement(242..859)
                     /gene="gmk"
                     /locus_tag="VKZ48_02245"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HLU76707.1"
                     /translation="MSAVKGLLFIVAAPSGAGKSTLVNAVLENDAELTLSVSYTTRTP
                     RPGEQDGREYHFIDSETFRCMRENGEFLESAEVHGNWYATSQKQITTLRNEGRDVMLE
                     IDWQGAQQVRRLFPDAIGVFILPPSLDELRRRLIGRAQDSEAVIQRRLAAAATEISHA
                     VEFDYVIINNNFEHARDDLAAIVRASRLAMPQQRVRHPELFNLKE"
     gene            complement(972..1835)
                     /locus_tag="VKZ48_02250"
     CDS             complement(972..1835)
                     /locus_tag="VKZ48_02250"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HLU76708.1"
                     /translation="MIQSMTGYATTIRELPQGTLTLELRSVNSRFLDVQFRIAEDLRA
                     AEPQLREQITARIRRGKVDCRMSYAAAPLAQVEAPLDPEVLKRLAQQAEQVRRYLPDA
                     TPLRVADVLRWPGVMREDNADDALREATAQAVEAALDELVAARAREGDKLRQMILERV
                     TAIRARLQELQPLIPEALAAYQEKLANKLREVLASADEERIRQEIAVFGVKIDVAEEL
                     SRLATHLDEVERVLAAGGAVGKRLDFLMQELNREANTLGAKSVSKELSAAALEFKLLI
                     EQMREQVQNLE"
     gene            1938..2885
                     /locus_tag="VKZ48_02255"
     CDS             1938..2885
                     /locus_tag="VKZ48_02255"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HLU76709.1"
                     /translation="MPSQANQPLPDGYQLQNYRITKVLSCGGFSIVYLAHDENEQPVA
                     IKEYLPSSLALRKEGDALPSVSEENLATFRYGMKCFFEEGRALARLSHPNLVRVLNFF
                     RANETVYLVMRYERGRTLQEHIQARRGAMKENFIRRVFTLLLNGLREVHTNKLLHLDI
                     KPANIYIRNDTTPVLIDFGAARQTLTTEGFKLNPMYTPGFASPEHYQKRDLLGPWSDI
                     YSVGASLFACLAGAAPQAADLRVERDRYVSATKLFAGKYSDQLLETIDWCLQLDHLQR
                     PQSVMALQKVLLRERDPEGPRKRSFVSNLRGTLMRLARR"
     gene            2901..3830
                     /locus_tag="VKZ48_02260"
     CDS             2901..3830
                     /locus_tag="VKZ48_02260"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HLU76710.1"
                     /translation="MKFSIYQESRQGPRKANQDRVAYCYSRDSLCMIVADGMGGHLHG
                     EVAAQIATQFIAEAFQRQATPRIEEPFRFLLDTIMNAHLAIIDYANVRGLLETPRTTV
                     VACIVQDGLAYWAHVGDSRLYHVRDGRIVMQTKDHSRVQMLVDSGRIREEAVAQHPER
                     NKIFNCLGQMAPPKIDLSRPTALHHGDTLLLCSDGLWGPLSGRQICEALLRDDIMTAV
                     PSLLDLAESRAGRDGDNLSAIAMTWAEELIDDEANWISTVEMEPSAYRTTVAQFGRNS
                     QVGQAGQEQGAYLSDEEIERAIAEIRNAIKKHS"
     gene            3952..4671
                     /gene="rph"
                     /locus_tag="VKZ48_02265"
     CDS             3952..4671
                     /gene="rph"
                     /locus_tag="VKZ48_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HLU76711.1"
                     /translation="MTRPSGRSPEQLRTVRITRGYTKHAEGSVLIEFGDTKVLCTASV
                     EEKVPPFLRDRNQGWLTAEYGMLPRSTNTRTDREAARGKQSGRTQEIQRLIGRSLRAV
                     VDLSALGQRTIQVDCDVLQADGGTRTASITGAFVAVHDAVSLLLAQQRIALSPIRDFV
                     AAVSVGVHGGVPLLDLDYAEDSACDTDMNVVMTGSGGFVEVQGTAEGAPFARTELDAL
                     LDLATRGIEQLVAAQRAALDL"
     gene            4677..5267
                     /gene="rdgB"
                     /locus_tag="VKZ48_02270"
     CDS             4677..5267
                     /gene="rdgB"
                     /locus_tag="VKZ48_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HLU76712.1"
                     /translation="MKRIVLASNNAGKLRELEQLLAPLGCEVVAQGALGVGEAEEPYG
                     TFVENALAKARHAARETGLPALADDSGICVDALGGAPGVHSARFAGAPKSDARNNEKL
                     VSLLSECTDRRAHYYCVAVYMRHADDPEPLIAEGRWHGEIIDTARGTGGFGYDPHFYL
                     PALGCTAAELSAEEKHALSHRGEALRRLVELLRARD"
     gene            5269..6462
                     /gene="hemW"
                     /locus_tag="VKZ48_02275"
     CDS             5269..6462
                     /gene="hemW"
                     /locus_tag="VKZ48_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HLU76713.1"
                     /translation="MAEVVPIQTNLLGPKLPAPPPLSLYVHLPWCARKCPYCDFNSHP
                     TRQAIPETEYADALIADLEQALPGIWGRRVYTVFFGGGTPSLFSAETIERLLSAFRAR
                     LQLSSDAEITLEANPGSAEAEKFRDYRAAGVNRLSIGIQSFNDRHLQVLGRIHGADEA
                     RRAIDIAQRHFDNYNLDLMYALPEQTLEEALADVREAVATGAPHLSCYHLTLEPGTPF
                     HHSPPPVPDEDAAADIHDAVVATLAAGGYTHYETSAHARAGHECRHNLNYWRFGDYLG
                     IGAGAHSKLSFHDRIVRETRHRLPKTYMESVRQGSAVHEQRTLTRRDLPFEYMMNALR
                     LSEGFAVAEYGERTGLPLTTVERPLREAEARGLIERTHERIKPTPRGRRYLNDLLTLF
                     LSGPV"
     gene            complement(6459..>6764)
                     /locus_tag="VKZ48_02280"
     CDS             complement(6459..>6764)
                     /locus_tag="VKZ48_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006405601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HLU76714.1"
                     /translation="TLVQSVGDAFLPAYVPIVERRRTQSYGERERDFQAYRRGRYVEF
                     NLVYDRGTLFGLQSGGRTESILMSLPPVVKWRYDWRPAAGTPEARLYSDFLRPRDWV"
BASE COUNT         1240 a         2179 c         2143 g         1202 t
ORIGIN      
        1 gtaagtaggg ctacctttgc acgcgtcgtt caactctgat ggacacgcga caacatttcc
       61 agcccgacat tgccggcggc cacttctcgc agcgcgatga cggtcggctt gtcgcgatcg
      121 ggttccacgg tcggcgtccc gccctgcgcc agttgacgcg cgcggtaggt cgccgccagg
      181 gtcagctgga agcggttggg gattttttcc aggcaatcat cgacagtgat acgcgccata
      241 actattcctt caagttgaac aactcgggat gccgcacacg ctgctgcggc atggcgagtc
      301 gtgaagctcg aacaatcgcc gcaaggtcgt cgcgggcgtg ctcgaagttg ttgttaataa
      361 taacataatc gaactcgaca gcatggctga tctcggtcgc tgccgccgcc aggcgacgct
      421 ggatgacggc ctcggaatcc tgtgcccgac cgatcagccg gcgccgcaat tcgtcgagcg
      481 aaggcggcag gatgaagacg ccgatcgcat ccgggaaaag gcgccgcacc tgctgtgcgc
      541 cctgccagtc aatttcgagc atcacgtcgc gtccttcgtt ccgcagcgtg gtgatctgct
      601 tctgcgaggt cgcataccag ttgccgtgca cctccgcgct ttccaggaat tccccgtttt
      661 cgcgcatgca acggaaggtc tcgctgtcga tgaaatgata ctcccgcccg tcctgctcac
      721 cgggtcgcgg cgtgcgggtg gtataggaga cggacagcgt cagttcggcg tcgttctcca
      781 gtaccgcgtt gacgagcgtg gatttgccgg cgccggaagg cgcggcaacg ataaacagca
      841 gacctttcac agcggacata cctgcatgga cgtagcgtgt agcgaagaaa tcataagcgc
      901 gcagacgggc attgtctccc gccggcgccg cgccgagggt gctcccggca cgggttcgtg
      961 atgtttgacg gctactcgag gttctggacc tgctcgcgca tctgctcgat gagcagcttg
     1021 aactcgagtg ccgcggcgga caattccttg gaaacggatt tcgcgccgag ggtattggcc
     1081 tcgcgattga gttcctgcat gaggaaatcg agtcgcttgc cgaccgcccc gcctgctgca
     1141 aggacgcgct cgacttcgtc cagatgcgtg gcgagccggg acaattcttc cgcgacatcg
     1201 atcttcacgc cgaatacggc gatttcctga cggatgcgct cttcgtccgc gctggccagt
     1261 acctcgcgca gtttgttggc cagtttctcc tgataggccg cgagcgcttc tggaatgagc
     1321 ggctgcaatt cctgcagacg cgcgcgaatg gcggtcacgc gttccagaat catctgccgc
     1381 agtttgtcgc cttcgcgcgc ccgcgccgcg accagttcgt cgagcgccgc ctccacggcc
     1441 tgtgcggtcg cctcgcgcag tgcgtcgtcc gcgttgtcct cgcgcatgac gccgggccag
     1501 cgcagcacgt ctgcgacccg cagcggcgtg gcgtcgggca gatatcggcg cacctgctcc
     1561 gcctgttggg cgagccgctt gaggacctcc ggatccagtg gcgcttccac ctgcgcgagc
     1621 ggagcggctg cgtaactcat gcggcaatcg actttgccgc gccggatgcg ggcagtgatc
     1681 tgctcccgca attgcggctc agcggcgcgc aaatcttccg caatgcggaa ttggacgtcc
     1741 agaaagcggc tgttgacgct gcgcaattcc agcgtcaggg tgccttgggg aagctcgcga
     1801 atcgtcgtcg cgtagccggt catgctttga atcatgcgta gcttgtcagg ttgtcggggc
     1861 gccgccgctt tactttgggg gggtaaagaa agacaatcct gcccgaaaaa accgtatctt
     1921 atcgcacacc ggacctcatg ccatcgcaag caaaccagcc tttgccggat ggctaccaac
     1981 ttcagaatta ccgaataacg aaggttctct cctgcggcgg gttctcgatt gtctacctcg
     2041 cgcatgacga gaacgagcag cccgtagcga tcaaggaata cctgcccagt tcgctcgccc
     2101 tgcgcaagga gggcgacgct ttgccgtcgg tgtcggaaga gaatctggcc accttccgct
     2161 acggcatgaa atgtttcttc gaggaaggtc gtgcgctcgc gcgcctgtcg caccccaacc
     2221 tggtgcgcgt gttgaacttt tttcgcgcca acgaaaccgt ttatctggtc atgcgctacg
     2281 agcgcgggcg cacgctgcag gagcatatcc aggcgcgtcg cggcgcgatg aaggagaact
     2341 tcatacgccg cgtgttcacg ttgctgctca acgggttgcg ggaggtccat accaacaagc
     2401 tgctgcatct ggacatcaag ccggcgaata tctacatccg taacgacacc acgccggtgt
     2461 tgatcgattt cggcgccgcc cggcagacct tgacgaccga ggggttcaag ctgaacccga
     2521 tgtacacgcc gggcttcgct tcgccggagc actaccagaa gcgcgacctg ctcgggccgt
     2581 ggagcgacat ctattcggtg ggcgcgagcc tctttgcctg tctggccggc gcggcaccgc
     2641 aggcggccga ccttcgcgtg gagcgcgacc gttatgtctc ggcgaccaaa ttgttcgccg
     2701 gcaaatattc cgatcagctg ctggaaacca tcgactggtg tctgcaactc gatcatttgc
     2761 aacgcccgca aagcgtcatg gcgctacaga aagtgctgtt gcgggagcgt gatccggaag
     2821 gaccgcgcaa acgttccttc gtgtcgaatc tgcgcggcac gctcatgaga ctggcacgtc
     2881 gctagaccga gaggctgcgg atgaaattca gcatctatca ggaaagcagg caggggccgc
     2941 gcaaggcgaa ccaggatcgg gttgcgtact gctacagccg cgattcgcta tgcatgatcg
     3001 tcgcggatgg catgggcggc catctccatg gcgaggtggc cgcccagatc gcaacccagt
     3061 tcatcgccga ggccttccag cgccaggcga ctcctcgcat cgaggaaccc tttcgcttcc
     3121 tgctcgacac catcatgaat gcgcacctgg cgatcatcga ctacgccaat gtgcgcggac
     3181 tcctggaaac gcctcgtacc accgtcgtcg cctgcatcgt ccaggacggt ctcgcctact
     3241 gggcgcatgt cggcgattcg cggctttatc acgtgcgcga cggccgcatc gtcatgcaga
     3301 ccaaggatca ctcgcgcgtg cagatgctgg tggacagcgg ccgcatccgc gaagaggctg
     3361 tggcacagca ccccgagcgg aacaagattt tcaactgcct gggccagatg gcgccgccca
     3421 aaatcgatct gtcccgcccc accgcgctgc atcacggcga taccctgctg ttgtgcagcg
     3481 acggactgtg ggggccgctg tccggcaggc agatctgcga agcgttgctg cgcgacgaca
     3541 tcatgaccgc cgtcccgagt ctgctcgacc tggccgagtc gcgcgccggg cgggacggcg
     3601 acaatctgtc cgctatcgcc atgacctggg cggaggagtt gatcgacgac gaggcgaact
     3661 ggatctccac cgtggaaatg gagcccagtg cctacaggac gacggtggcg cagttcggcc
     3721 gcaacagtca ggtggggcag gccggtcagg agcagggcgc ctatctgagc gacgaagaga
     3781 tcgagcgcgc cattgcggaa atccgcaatg cgatcaagaa gcactcttga gccggatcgc
     3841 cggacgccaa aatccgcgaa aatcagcaaa gcaccgaccg cttctgatat ttaatcaaag
     3901 caggcgccga acgcctgcgc cgacccgttt cattcagcca gaacgatatc catgacccga
     3961 ccaagcgggc gctcgcccga acaactccgc accgtccgca tcactcgcgg ctacaccaag
     4021 catgccgaag gctcggtatt gatcgagttc ggcgacacca aggtgctgtg caccgccagt
     4081 gtcgaagaaa aagtgccgcc ctttctgcgc gatcgcaatc agggatggct gacggccgag
     4141 tacggcatgc tgccgcgatc gaccaacacg cgcacggacc gggaggcggc gcgcgggaag
     4201 cagagcggac gcacgcagga aatccagcgc ctcatcggtc gctcgctgcg cgcggtggtg
     4261 gatttgagcg ccctgggtca acgcacgatc caggtggatt gtgacgtcct ccaggccgac
     4321 ggcggcacgc gtacggcaag catcaccggc gctttcgtgg ccgtgcacga cgcggtgagc
     4381 ctgctgttag cgcaacagcg cattgcgctg tcgccgatac gcgacttcgt cgccgccgta
     4441 tcggtcggcg tgcatggcgg cgtgccgttg ctcgacctcg attacgccga ggattccgcg
     4501 tgcgacaccg acatgaatgt ggtgatgacg ggcagcggcg ggttcgtgga agtgcagggc
     4561 acggccgaag gcgccccttt cgcgcgcacc gaactggacg cgttgctgga tctcgcgacc
     4621 cgcggcatcg aacaactcgt cgccgcgcag cgcgcggcac tggacttgtg agcacaatga
     4681 aacggatcgt gctggcttcg aacaacgccg gcaagctgcg cgaactcgaa caactgcttg
     4741 cgccgctggg ttgcgaggtc gtcgcgcaag gggcgctcgg cgtcggagaa gccgaagagc
     4801 cgtacggcac tttcgtcgag aatgccctcg ccaaagcgcg tcatgcggcg cgggaaaccg
     4861 gtctgccggc gcttgccgac gattccggca tttgcgtgga tgcgctgggc ggcgccccgg
     4921 gcgtgcattc cgcccggttc gccggcgcgc ccaaatcgga tgcgcgcaat aacgaaaagc
     4981 tggtgtcgct gctgtccgaa tgcacggatc ggcgagcgca ttactactgc gtcgccgtct
     5041 acatgcggca tgcggacgat cccgaaccgc tgatcgcgga aggccgatgg cacggcgaga
     5101 tcatcgatac ggcgcgcggc accggcggtt tcggctacga cccgcacttc tatctgcccg
     5161 cgctcgggtg cacggccgct gaattgagcg cagaggaaaa gcatgcgctg agccaccgcg
     5221 gcgaggcgct gcgccgttta gtcgaactgt tgcgggcgcg cgattgagat ggcggaagtc
     5281 gtccccatac agaccaacct gctcgggccg aagctgccgg cgccgccgcc cctgtcgctg
     5341 tacgtgcacc tgccctggtg cgcgcgcaaa tgtccctatt gcgatttcaa ctcgcatccc
     5401 acgcgccagg ccattcccga aaccgagtat gccgatgcgt tgattgccga tctggaacag
     5461 gcgctgcccg gcatctgggg gcggcgcgtc tatacggtgt ttttcggcgg cggcacaccc
     5521 agcctgttct cggcggaaac catcgaacgt ctgctgagcg cctttcgcgc gcggctgcag
     5581 ttgtcctccg atgcggagat cacgctggag gccaatcccg gcagcgccga ggcggagaag
     5641 ttccgcgatt accgcgccgc cggcgtcaac cggctgtcga tcggcatcca gagcttcaac
     5701 gatcggcact tgcaggtgtt ggggcgcatc cacggcgccg acgaagcgcg ccgcgccatc
     5761 gacatcgcgc agcggcattt cgacaactac aacctcgacc tcatgtacgc cctgccggaa
     5821 cagacgctcg aggaagcgct cgcggacgtg cgcgaagcag tggcgaccgg cgcgccgcac
     5881 ctgtcctgct atcacctcac gctggagccg ggcacgccct ttcatcactc gccgccgccc
     5941 gtccccgacg aggacgcggc ggccgacatc catgatgccg tcgtcgccac cctggcggca
     6001 ggcggctaca cgcactacga aacctccgcg cacgccaggg cgggacacga gtgccgccac
     6061 aatctcaatt actggcgctt cggcgattac ctgggcatcg gcgccggcgc gcattccaag
     6121 ctgtcgttcc acgaccgcat cgtgcgcgag acgcgccatc gtctgccgaa gacgtatatg
     6181 gaaagcgtgc ggcagggcag tgcggtgcat gaacagcgca cgctgacgcg tcgcgacctg
     6241 cctttcgaat acatgatgaa cgcgctgcgt ctgagcgagg gatttgccgt agccgagtac
     6301 ggcgagcgta ccggactgcc gctgacgacg gtggaacgac cgctgcgcga ggcggaggcg
     6361 cgcggcctga tcgaacgcac ccatgagcgc atcaagccca cgccgcgcgg ccggcgctat
     6421 ctcaacgatc tgctgaccct gtttttgagc ggaccggtct agacccaatc gcgcggccgc
     6481 aggaagtcgc tgtacagccg cgcttccggc gtgccggccg ccggccgcca gtcgtagcgc
     6541 cacttcacca ccggcggcag cgacatcagg atcgattcgg tgcgcccgcc cgattgcagg
     6601 ccgaacaggg tgccgcggtc gtacaccaga ttgaattcca catagcggcc gcgccggtag
     6661 gcctggaaat cccgctcgcg ttcgccgtag gactgcgtgc ggcggcgttc gacgatgggc
     6721 acataagcgg gcaggaaggc gtcgcccacg ctctgcacca gcgt
//
LOCUS       DATGOU010000042        18790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3217231, whole genome shotgun sequence.
ACCESSION   DATGOU010000042 DATGOU010000000
VERSION     DATGOU010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 18790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18790
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3217231"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="VKZ48_02285"
     CDS             <1..1352
                     /locus_tag="VKZ48_02285"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HLU76715.1"
                     /translation="RDLSGNLSIATQSYLKQDALVRYNSQSRWWSAYGRVQKFQVLQD
                     PLNPLPGPYERVPQLVLNVLNQQSTYLDLGLRAEFVAFDHPTRQTGQRLIAYPSIALP
                     LSGPGAYIKPKVGVHATWYNLSDFDNLDERRPTRSLPIASVDTGLVFERDMNWLGVDW
                     LQTLEPRLYYLYVPYRDQDEIPLFDTTLADFNYGQLFSENAFVGGDRIADANQLTVGV
                     SSRFISPSTGQEMLRAFVGQRYYFDDRFVTLQPGDSPPTSDASPLLASVAGRVAPDWF
                     AETTVQYGWDDRELERLNAGVRYSPAPASVVNLSYRYTHANQTAINEEISAIDLSAQW
                     PLSRRWYGMARINYDTNGGGMVERLLGLEYNADCWILRLVFHDFKTATDQETRVFFLQ
                     LELNGFSRVGSNPFDTLRRNIPGYTPTNLIREGESNVFDRGDAVSPWSTNGSGTYAY"
     gene            1342..2658
                     /locus_tag="VKZ48_02290"
     CDS             1342..2658
                     /locus_tag="VKZ48_02290"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLU76716.1"
                     /translation="MHTKLLNRFALACMLVSGSALAVAQPAAERITLADRIVAVVNNE
                     VITQRGLEERVQTVVRQLERSGAALPPRDVLERQILERMIVDHAQLQFARESGIRVDD
                     ATVDAAIARIATNNQLSVAEFRETLARDGIPYDGFREDIRAEITLSRLREREIYGRIQ
                     VSESEVDNFLAEQQDQDVDAEYLLSHILVRVPERATPEQLAQRMSRAEEVRKRALAGE
                     DFAQLAVTFSDAPDGLEGGDMGWRGPAKLPELFVQAVAPLQAGGVSEVLRSPAGYHII
                     KLRDRRGGSPLQAMTAEQTHVRHILVRTNELVSEEEAQRRLSVLRERIVQGGDFAQLA
                     RLNSDDASAANGGDLGWILQGDTVPEFEQAMNALQPGEISEPIRSPFGYHLIQVVERR
                     VADVSGDRKRMEARRILRERKAEEAYQEWVRQLRDRAFVEYRLEEG"
     gene            2668..3633
                     /gene="pdxA"
                     /locus_tag="VKZ48_02295"
     CDS             2668..3633
                     /gene="pdxA"
                     /locus_tag="VKZ48_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HLU76717.1"
                     /translation="MAGSQQLPRIAVTAGEPAGIGPDICLALADRAFDARLVVLADRS
                     LLEARAGALGRDLPATLDIRHIPLQAPATAGRLDPRNGRHVLALLDEAIAGCLDGRYD
                     AMVTAPVQKSVINDAGIAFTGHTEYLADRAGSDVVMMLAGGGLRVALATTHLAVKNVP
                     AALNVPMLRRTLQIIDGDLRRRFGIARPVILVAGLNPHAGESGYLGREEIEVITPALE
                     SLRAEGLDVRGPLPADTLFTPQQLEGADCVLAMYHDQGLPVLKYASFGHGVNVTLGLP
                     FIRTSVDHGTALDLAGTGKADAHSLYEAVETAIAMVRHERAASRT"
     gene            3713..4492
                     /gene="rsmA"
                     /locus_tag="VKZ48_02300"
     CDS             3713..4492
                     /gene="rsmA"
                     /locus_tag="VKZ48_02300"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HLU76718.1"
                     /translation="MKQHIPRRRFGQNFLVDQGIIRAIVEAIAPRPDDVLVEIGPGLG
                     ALTGPLLERMPHLHVIEIDRDIVTRLQKQYPPERLTIHAGDVLDFDFAKLPGRFRLIG
                     NLPYNISTELLFRLIPYADRMIDAHFMLQREVVDRMVAGPSTAEYGRLSVMLQYRFDM
                     LKLIDVPPTSFRPAPKVDSAVVRMHPRDKEATCLVDETLLSRVVTAAFTKRRKTLRNA
                     LAGVIAPERLQSLGIDPQLRPENLGVADYLAIVGALDGKTG"
     gene            complement(4514..5287)
                     /locus_tag="VKZ48_02305"
     CDS             complement(4514..5287)
                     /locus_tag="VKZ48_02305"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HLU76719.1"
                     /translation="MQYKLPFLIESPPELEADTPHTSRVRWINLAGRMLSYSFRRSSR
                     RTIGLVIDERGLQAAAPKWTPIAEVEAFIREKERWILKHLPHNRLQRPPFLWQTGARL
                     PYLGREIVVHCNGHAQAIQLRGDLLEVGLHAATPHALREAVLAWLKNEALALYRARID
                     ALAPRLDVAPPEVGLSQARTQWGSCTRKRDGRTRILLNWKLIHFELPLIDYVAAHELA
                     HLRHMNHSAAFWHEVARLYPDFREAREALRRRGHLIPEL"
     gene            complement(5297..6064)
                     /locus_tag="VKZ48_02310"
     CDS             complement(5297..6064)
                     /locus_tag="VKZ48_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HLU76720.1"
                     /translation="MNLLRSTLYQLILLLVVIPYSFIVLAMAPLPRKARHRVIAAWPA
                     FAAWLAQYVLGIRYVVRGRENIPAEPLVILSKHQSAWETLAYSAIFPPHVYVMKRELL
                     WLPFLGWGLALMSPIAINRANRKAAMARLIALGGERLRQGFSIMIYPEGTRIPVGQRG
                     HYRQGGAVLAVNNGVRVLPVAHNAGLLWPKNSFVKHPGTVTVVIGKPIETAGRRPDEV
                     MREVEEWIEGEVQRLTTPARTVESAAAPNLEGDATGS"
     gene            complement(6061..6597)
                     /gene="gmhB"
                     /locus_tag="VKZ48_02315"
     CDS             complement(6061..6597)
                     /gene="gmhB"
                     /locus_tag="VKZ48_02315"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HLU76721.1"
                     /translation="MKLIILDRDGVINHDSPQFIKSPDEWIPIPGSVEAIARFTQAGY
                     RVVVATNQSGISRGLFDMATLNAIHDKMHRQVAHAGGRIDAIFYCPAGADSNSPYRKP
                     NPGMLVEIGERLNMPLEDVPAVGDALRDLQAAAAVKAQPILVLTGKGVSTQHENLPPG
                     TRIFPDLAAVADALLGSP"
     gene            complement(6656..8728)
                     /gene="glyS"
                     /locus_tag="VKZ48_02320"
     CDS             complement(6656..8728)
                     /gene="glyS"
                     /locus_tag="VKZ48_02320"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HLU76722.1"
                     /translation="MTKAALLIELLTEELPPKALRQLGDSFATALVADLRAHAFIDAA
                     AAHRTFASPRRLAVLVQDVSAQAPDREVLVKGPSLKVGLDAAGKPSPALLGFARKQGV
                     DVEVLERLQDGKNEVFAYRKTERGSRLADVLQEIIAAALKRLPIPKMMRWGAGDAEFV
                     RPVRGLVALHGETVVPTSLFGLQAGRRTCGHRFLASRPLDIASADTYESTLERDGKVI
                     ACFERRLALIRESLQEAAGEAVIAADEALYAEVTALVELPVVYEGRFDAAFLEVPQEC
                     LMLTMRQNQKYFPLIGKDGRLLNRFLLVSNMEAADPGEIVRGNERVLRARLADAQFFY
                     DQDRKQPLATLVPKLADVVYHNKLGSQLQRVERIESLASAIAGQLDADQALATRAAHL
                     AKADLLTGMVGEFPELQGIMGRYYARHDNEAEAVANAIEAHYRPRFAGDELPGDAIGD
                     AVALGDKLDTLVGIWGIGLAPTGDKDPFALRRAALGVARILVEHALPLDVLELLELAE
                     RQFAPGVIAASTVADVHAFLLDRLRSYLRDRDFAADEIEAVIASSPSRLDTIVPRLAA
                     VRAFRQLPEAESLAAANKRIHNILKKATDPHAAAPQPELLQEPAEQALHDTLQSLAPG
                     IDAHMQARRYTEALSALAGARNAVDRFFDEVMVMTDDAAVRANRLALLGALEDLMNRV
                     ADISRLAA"
     gene            complement(8721..9617)
                     /gene="glyQ"
                     /locus_tag="VKZ48_02325"
     CDS             complement(8721..9617)
                     /gene="glyQ"
                     /locus_tag="VKZ48_02325"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HLU76723.1"
                     /translation="MHTFQELILALQGYWAQRGCTLLQPYDLEVGAGTFHTATFLRAI
                     GPEPWNAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVVLKPSPDDIQDLYLDSLRAIGL
                     DLSKHDVRFVEDDWESPTLGAWGLGWEVWLDGMEVTQFTYFQEIGSLPCKPVLGEITY
                     GLERLAMHLQGKENVYDLVWAEGPDGSVTYGDVYHQNEVEQSRYNFEEANAPWLLELF
                     GEFESEAKRLIAAGLPLPGYEMVMKCSHTFNLLDARGAISVTERAAYIGRVRALARQV
                     AQAYYESRERLGFPLVRRTAHD"
     gene            complement(9799..9993)
                     /locus_tag="VKZ48_02330"
     CDS             complement(9799..9993)
                     /locus_tag="VKZ48_02330"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HLU76724.1"
                     /translation="MTDAEVDTLHHHINSLKAEHRQLDTLVNRLCTTQVYGDGRLQEL
                     KLRRLRLKDRLAALERQLES"
     gene            complement(10092..11567)
                     /gene="lnt"
                     /locus_tag="VKZ48_02335"
     CDS             complement(10092..11567)
                     /gene="lnt"
                     /locus_tag="VKZ48_02335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HLU76725.1"
                     /translation="MIPPVRRKTLVAVAAGALSVAGFGDLPLWPLSLLAFAALLRLWH
                     TAETPRQAAGLGYAWGVGAFLVGVSWVYVSLHDFGMMPAPLAAVATLLFCLYLALFPA
                     AAGYLQAHLPGSPALRLMLYMPACWVLTEWLRGWLLTGFPWLAFGYSTVDTPLAAYAP
                     LVGVYGLSLLAAIAMGALVAFMQRGHERIIGAAVFVALIGAGLTLRSVTWTRPVGAPI
                     SVSLLQGNIPQELKFVPGRYEQTLHTYARLARTAPAQLIVLPETAIPRFLDQVDPAYL
                     EQLADIARVQSGDMLVPAPLRDANGGYYNAAVSLGTAPMQRYAKVHLVPFGEFIPPGF
                     GWVLNVLQIPLSDFSAGATTQRPLHVAGERVALNICYEDAFGEEIIRQLPEATLLVNV
                     SNVAWFGDSLAPEQHLRIARMRALETGRTMLRATNTGITAIIEADGTVQRRLPPFTEG
                     ALLGSAQGREGATPYVRFGNWPALIWALGALGLGVLHRRRA"
     gene            complement(11564..12400)
                     /locus_tag="VKZ48_02340"
     CDS             complement(11564..12400)
                     /locus_tag="VKZ48_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HLU76726.1"
                     /translation="MDEDQTHKPSLLERLGALIMREPEDRNQLIALLHSAHERNLLDA
                     DALTMIEGALQVSEVKVSDVMIPRSQMCVIDINDSPEEFIPQVIATAHSRFPVIDRDR
                     DHVIGILLAKDLLRYYAGDDEFNVRDMLRPAVFVPESKRLNILLREFRVSRNHMAIVV
                     DEYGGVAGLVTIEDVLEQIVGDIEDEHDFDEIQDNIMRERSGRWRVKAQTEIADFNEQ
                     FATDFSDEEHDTVGGMVISRFGRLPKRGESITIDGFRFLVQRADSRRVHTLLVERAQE
                     TS"
     gene            complement(12428..12856)
                     /gene="ybeY"
                     /locus_tag="VKZ48_02345"
     CDS             complement(12428..12856)
                     /gene="ybeY"
                     /locus_tag="VKZ48_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HLU76727.1"
                     /translation="MQVQYAVPRSGLPAARSVRRWALAALEQSAQATVRFVGTREGRA
                     LNRQYRGRDYATNVLTFTYGQHEGALLGDIVLCAPVVAREAREQRKALRGHYAHLVVH
                     GMLHLQGYDHERTREAQHMERREIAVLRELGFDNPYDPAG"
     gene            complement(12883..13833)
                     /locus_tag="VKZ48_02350"
     CDS             complement(12883..13833)
                     /locus_tag="VKZ48_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HLU76728.1"
                     /translation="MTLEPADNQRLAHLCGALDENLRQMEAGLDVSIARRGDRFSVRG
                     TPEQAQRAADMLRKFYAGATRELSIDDVQLGIVEAMQPVAPEKQGPALLTRRPDLHGR
                     TPRQRQYLQNIVENDITFGVGPAGTGKTYLAVACAVDALERDAVKRIVLVRPAVEAGE
                     RLGFLPGDLAQKVDPYLRPLYDALYDLMGFDKVGKMFERGAIEIAPLAYMRGRTLNHS
                     FIILDEAQNTTPEQMKMFLTRIGFGTKAVITGDVTQIDLGKGQKSGLVEARHVLADVR
                     GIAFTVFESEDVVRHPLVARIVNAYEHYEVGAGLRPPRRD"
     gene            complement(13884..15236)
                     /gene="miaB"
                     /locus_tag="VKZ48_02355"
     CDS             complement(13884..15236)
                     /gene="miaB"
                     /locus_tag="VKZ48_02355"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HLU76729.1"
                     /translation="MSKRVYIKTFGCQMNEYDSDKMADVLHAAEGLEPTDRPEEADVI
                     LFNTCSVREKAQEKVFHDLGRVRALKQQNPDLVIGVGGCVASQEGEAIVKRAPFVDVV
                     FGPQTLHRLPELIAERRRSGKPQVDISFPEIEKFDHLPPARVEAATAFVSIMEGCSKY
                     CSYCVVPYTRGEEVSRPFEDVIVEIADLADRGVKEVTLLGQNVNAYRGRLPDGEIADF
                     AMLLEYVADVPGIERIRYTTSHPKEFTQRLIDAYAKLPKLVSHVHLPVQAGSDRVLAA
                     MKRGYTVLEFKSIVRRLRAARPDICISSDFIVGFPGETERDFEQTMKLVDDVGFDASF
                     SFIYSARPGTPAAELRDDTPHEVKVERLMRLQAKLEANGRAVNEAMVGTVQRVLVEGR
                     AKKDENELAGRTDNNRVVNFPAPERLIGRFVDVQIETAYAHSLRGRMHLREDALAIAT
                     "
     gene            complement(15239..15634)
                     /locus_tag="VKZ48_02360"
     CDS             complement(15239..15634)
                     /locus_tag="VKZ48_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76730.1"
                     /translation="MRLFLLRSLLAAVLGAALSSAAWAQFRQIPADAKPARMTVLQQN
                     VVQLDDKQYLLAPGALIRDTSNIIVLPTMVPTRAPVRYQLDNMGQIKRVWLLSAAEAA
                     QVEAQKPVPLPAPIQENKDEPRGDGLDDQ"
     gene            complement(15835..15910)
                     /locus_tag="VKZ48_02365"
     tRNA            complement(15835..15910)
                     /locus_tag="VKZ48_02365"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15875..15877),aa:Met,seq:cat)
     gene            16078..17091
                     /locus_tag="VKZ48_02370"
     CDS             16078..17091
                     /locus_tag="VKZ48_02370"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HLU76731.1"
                     /translation="MKVCVVGAGAIGGLVAVRLALGGQDDVTVVVRGANLEAIRANGM
                     KLIEHDGTERVAKNIRACTWDDAGPQDVVILGMKAHQVEPILPDLDKILHEDTIIIPM
                     QNGIPWWYFQGQGDVPHANQTVESVDPGGKIKSAIDPRRILGCVVYPASELTAPGVIH
                     HIEGDRFPIGELNGAITERAQRVSEMFQRGGLKAPVLENIRSEIWLKLWGNLTFNPIS
                     ALTHSTLVDICQFPLTRELAAAMMTEAQTVAHKLNITFRVPLEKRIAGAEKVGKHKTS
                     MLQDVEAGRDPEIDALVGSVIELAKLTDTPVPHIETTFALVKLLAKTMQEEKVLVRAQ
                     PLG"
     gene            17125..18300
                     /locus_tag="VKZ48_02375"
     CDS             17125..18300
                     /locus_tag="VKZ48_02375"
                     /inference="COORDINATES: protein motif:HMM:NF005788.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF family hydroxylase"
                     /protein_id="HLU76732.1"
                     /translation="MPPSISTDFDVLIAGAGLVGASFALALRDSGLRVGIIEPAEPSP
                     PGEAWDSRIYALNPANIDFLQTLGAWQRMDPARLQPVGRMQIYGDDQARLDFSAYESG
                     VAALAYILESGRLQYALWESLRDAPNIELLTGARCADADFESAAARIRLDDGRTLRTE
                     LLVGADGANSWVRRVARLQAQVHAYEHLGVVANFDCAKPHGGTAFQWFRHDGILAYLP
                     LAGQRMSMVWSTPDEHGRELLALPHEQLAARVAEAGRHVLGELRVITPPQAFPLRKMR
                     LPDVVAPRVALIGDAAHVVHPLAGQGVNLGFGDAAALARLIIEREPFRRCGDMAILRR
                     YQRERAEPVAAMGAVTHGLQRLFAAPGSWVAALRNRGLAYTDRTTVIKNLLVRQALG"
     gene            18320..>18790
                     /locus_tag="VKZ48_02380"
     CDS             18320..>18790
                     /locus_tag="VKZ48_02380"
                     /inference="COORDINATES: protein motif:HMM:NF021896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HLU76733.1"
                     /translation="MLRILLCALTLSLSAPAFADEAAIKKVIEARMQGAKVEKITKTP
                     YSGLYEVQIGNEVLYTDAQANYIFVGNVHDGQTLENLTEARLQELNKVDFDRFPLDQA
                     IKLVNGKGTRQLVYFSDPNCPYCKRMDKELQAIEDLTVYVFLYPILSPDSMAKSQ"
BASE COUNT         3333 a         5915 c         6118 g         3424 t
ORIGIN      
        1 tccgagatct ttcgggcaac ctgtccatcg ccacgcagtc ctatctgaag caggatgcac
       61 tggtgcgata caactcccag tccagatggt ggagtgcgta cggacgggtg cagaaatttc
      121 aggtcctgca ggaccccctg aatcccttgc ccgggcccta tgagcgcgtg ccgcagttgg
      181 ttctgaacgt gttgaatcag caatcgacct atctcgatct gggattgcgg gccgaattcg
      241 tggccttcga tcatccaact aggcagaccg ggcagcgtct gatcgcctat cccagcattg
      301 ccttgccgct gtccgggccg ggtgcctata tcaagccgaa ggtcggggtg catgcaacct
      361 ggtacaacct gagcgacttc gacaatctgg atgaacggcg gccgacgcgt tcgctgccca
      421 ttgcgagcgt ggataccggc ctggtgttcg aacgcgacat gaactggctg ggcgtcgact
      481 ggctgcagac cctcgagccg cggctctact acctctatgt gccgtaccgc gatcaggacg
      541 agattccgct gttcgacacg accctggccg atttcaatta cggtcaactc ttctcggaga
      601 atgcgtttgt cggcggcgac cgcatcgcgg acgcgaacca actgacggtc ggggtcagca
      661 gccgtttcat atcgcccagt acgggccagg aaatgctgcg cgcctttgtc ggccagcgtt
      721 actactttga cgatcggttc gtcactttgc aaccgggcga ttcaccgccc accagcgacg
      781 cgtcgccgct gctcgcgagc gtggcggggc gcgtcgcgcc cgactggttc gcggagacga
      841 ccgtccaata cggttgggac gatcgtgagt tggagcgcct gaatgcaggg gtgcgttact
      901 ctccggcacc ggccagcgtg gtcaatcttt cttatcgcta cactcacgcc aaccagacgg
      961 ccatcaacga ggaaatcagt gcgatcgatc tgtcggcgca atggccgctc agccggcgct
     1021 ggtacgggat ggcccggatc aactacgaca ccaacggggg cggcatggtc gaacggttgc
     1081 ttggccttga atataatgcc gactgctgga ttctccggct ggtgtttcac gacttcaaaa
     1141 cggctaccga tcaggaaacg agggtgttct tcctgcaact cgaactcaac ggtttttcgc
     1201 gcgtcggatc gaatcctttc gatacgctgc gacggaatat ccccggctac acgccgacga
     1261 acctcattcg tgagggggag agcaatgttt ttgatcgggg agacgccgta tcgccctggt
     1321 ccacgaacgg cagcggcacc tatgcatact aaattgctga atcgtttcgc cctggcctgc
     1381 atgctggtgt cgggcagcgc cctggctgtc gcccagcctg cggccgagcg catcacgctg
     1441 gccgatcgca tcgttgcggt ggtcaacaac gaagtgatta cccagcgcgg gctggaagag
     1501 cgggtgcaga ccgtggtccg gcaactggag cgcagcggcg ccgccctgcc gccgcgcgac
     1561 gtgctcgagc ggcagattct cgaacgcatg atcgtcgatc atgcgcaact gcagttcgcg
     1621 cgggaatccg gcattcgcgt ggacgatgcg accgttgacg cggcgattgc gcgtatcgcg
     1681 accaacaacc agctgagcgt cgccgagttc cgcgagaccc ttgcgcgcga cggcattccc
     1741 tatgacggct tccgcgaaga catacgcgcg gaaatcacgc tctcgcgcct gcgtgagcgg
     1801 gagatctacg gccgcattca agtgagcgag agcgaggtcg acaacttcct cgctgagcag
     1861 caggaccagg acgtcgatgc ggaatacctt ctgtcccaca tcctggtgcg cgtgcccgag
     1921 cgggcaacgc ccgagcaatt ggcgcagcgg atgagccgtg ccgaggaagt gcgcaaacgc
     1981 gcgctggcgg gcgaggattt tgcgcagctg gccgtgactt tctccgacgc gccggacgga
     2041 ctggagggcg gcgacatggg ttggcgcggg ccggccaagc tgccggaact cttcgtccag
     2101 gccgttgccc cgctgcaggc gggtggcgtc agcgaagtgc tgcgcagccc tgccggctat
     2161 cacatcatca aactgcgcga tcggcgcggc ggcagtccgc tgcaggccat gaccgccgag
     2221 cagacgcatg tgcgtcacat cctcgtacga accaacgaac tggtgtccga ggaagaggcg
     2281 caacgccggc tcagtgtgct gcgcgagcgc atcgtgcaag gcggggactt cgcgcaactc
     2341 gcgcgtctca actccgacga tgccagtgcg gcgaacggtg gcgatctggg ctggattctg
     2401 cagggcgaca ccgtgcccga gttcgaacag gcaatgaatg cgctgcaacc cggtgaaatc
     2461 agcgagccga tccgctcgcc tttcggttac cacctcatcc aggtcgtcga acggcgtgtc
     2521 gccgatgtaa gcggcgaccg caagcgcatg gaagcgcgcc gcattctgcg cgaacgcaag
     2581 gccgaagagg cctatcagga atgggtgcgg caattgcgcg accgcgcctt cgtcgaatat
     2641 cggctcgaag agggctgatc ccgcatcgtg gccggatcgc aacaactgcc gcgcattgcc
     2701 gtcactgccg gtgagccggc cggtatcggt ccggacatct gcttggcatt ggccgaccgg
     2761 gcattcgatg cccgactcgt cgtgcttgcc gaccgatcgt tgctcgaagc acgcgccggg
     2821 gcgctggggc gcgatttgcc tgccacgctc gatatccggc acattcctct ccaggcgccc
     2881 gcaacggccg gccgcctcga tccgcgcaac ggccgccatg tactggcgct gctcgatgag
     2941 gcgatagccg ggtgtctcga tgggcgttat gacgcgatgg tcaccgcccc ggtgcagaaa
     3001 agcgtcatta acgatgcggg catcgctttt accggccata ccgagtatct ggctgatcgg
     3061 gccggcagcg atgtcgtcat gatgctggcc ggcggcggat tgcgcgtcgc gctcgccacc
     3121 acccatctgg cagtcaagaa cgtacccgct gcgctgaacg tacccatgct gcgccggacg
     3181 ctacagatta tcgacggcga cttgcgccga cgcttcggca ttgcgcgccc tgtcatcctg
     3241 gtcgccggtc tgaacccgca tgccggcgaa agcggctacc tcggtcggga ggaaatcgaa
     3301 gtcattacgc ctgcgctcga atcgctgcgc gccgaagggt tggatgtgcg cggtcccctg
     3361 ccggcggaca cccttttcac gccccagcag ctcgagggcg cggattgcgt gctcgcgatg
     3421 tatcacgacc agggtctgcc cgtattgaaa tacgcaagct ttggccacgg cgtcaacgtc
     3481 accctcggcc tgcctttcat tcgtacctct gttgaccacg gtaccgctct ggatctggcc
     3541 ggtacgggca aagccgatgc gcatagtctg tacgaggccg tggagaccgc gatcgccatg
     3601 gtgcggcatg agcgcgccgc gtcgcgcacg tgaaaggtca tactccacgt cgtcgtttta
     3661 gttccggcat aacgcgcttg cgcgcattag ccttcactga aacgccggcg ccatgaaaca
     3721 acacattcca cgccgtcgtt ttggccaaaa cttcctggtc gaccagggca tcatccgcgc
     3781 gatcgtcgag gcgattgcgc caaggccgga tgacgtcctg gtagagatcg gcccgggcct
     3841 cggtgcgctg accgggccgc tgctcgaacg catgccgcat ctgcacgtca tcgaaatcga
     3901 tcgcgacatc gtgacacgat tgcagaaaca gtatccgccg gaacggctga cgatccatgc
     3961 gggcgatgtg ctggacttcg atttcgcgaa gctgccgggc cgtttccgac tgatcggcaa
     4021 tctgccttac aacatttcca cggaactgct gttccggctc atcccctacg ccgaccggat
     4081 gatcgatgcg catttcatgc tgcagcgtga agtggtcgat cgcatggtcg ccgggccgtc
     4141 gacagccgag tacggccgtc tgtcggtcat gctgcaatac cgcttcgata tgctgaagct
     4201 gatcgacgtg ccgccgacgt ccttcaggcc ggcgccgaaa gtcgattcgg ccgtcgtacg
     4261 catgcacccc cgtgacaaag aggcgacctg tctcgtggac gagaccttgc tgtcgcgggt
     4321 ggtgaccgcg gctttcacca agcggcgcaa aaccttgcgc aatgcgctgg ccggcgtgat
     4381 cgcgcccgag cgcttgcagt cactgggcat cgatccgcaa ctgcgtccgg aaaatctcgg
     4441 cgttgccgac tatctggcga tcgtcggcgc tttggacggc aagacgggct gaccggggac
     4501 ggctagccga ggctcagagt tccggaatga ggtggccgcg ccggcgcagc gcctcacgtg
     4561 cctcacggaa gtcgggatag agccttgcca cctcgtgcca gaaagcggcc gagtgattca
     4621 tgtgccgcaa atgcgccagt tcgtgcgcgg cgacgtagtc gatcaacggc aactcgaagt
     4681 ggatcagctt ccagttgagc aggatgcgcg tgcgaccgtc gcgcttgcgt gtgcaactgc
     4741 cccattgcgt acgcgcctgt gacaggccca cttccggcgg cgccacatcc agacggggtg
     4801 ccagtgcgtc gatgcgcgcg cgataaagcg ccagcgcttc gtttttgagc caggcgagca
     4861 ccgcttcgcg caaggcgtgc ggcgtcgcgg catgcaaccc gacttccagc agatcgccgc
     4921 gcaactgtat ggcctgcgca tggccgttac agtgcacgac gatctcgcgt cccagatacg
     4981 gcaagcgcgc gccggtttgc cagagaaagg gcggacgctg cagccggttg tgcggcagat
     5041 gtttgagaat ccagcgttcc ttctcgcgga tgaaggcttc gacttcggca ataggcgtcc
     5101 acttcggggc ggcagcctgc aggccgcgct cgtcgatgac caggccgatg gtgcgccggg
     5161 aggaacggcg gaaggaatag gacagcatcc ggcccgccag gttgatccag cgtactcgac
     5221 tcgtgtgggg ggtgtccgcc tccagttccg gtggcgattc gatcaggaac ggcagtttgt
     5281 attgcatcgg tggtgctcag gagccggtgg cgtcgccttc caggttcggc gctgcggcgg
     5341 attcgacggt gcgcgccggc gtggtcaggc gttgcacttc gccttcgatc cactcttcca
     5401 cttcgcgcat gacctcgtcg ggacggcggc cggccgtctc gatcggtttg ccgatgacca
     5461 ctgttaccgt gcccggatgt ttcacgaagg aattcttcgg ccacagcagt ccggcgttgt
     5521 gagcgaccgg cagcacgcgc accccgttgt tgaccgccag tacggcaccg ccctggcggt
     5581 agtgcccgcg ctgaccgacc ggaatgcgcg tgccttccgg atagatcatg atcgaaaagc
     5641 cctgccgcag ccgctcgccg cccagcgcga tcaaccgcgc catggcggct ttgcgattcg
     5701 cccggttgat ggcgatggga ctcatcagcg cgagccccca gccgagaaag ggcagccaca
     5761 gcaactcgcg cttcatgacg taaacgtgcg gcggaaagat ggcgctgtag gccagcgttt
     5821 cccaggccga ctgatgcttc gacagaatga ccagcggttc cgccggaatg ttctcgcggc
     5881 cgcgcacgac gtagcggatg ccgaggacgt actgtgcaag ccaggcggcg aaggccggcc
     5941 atgcggcgat gacacgatga cgcgccttgc gcggcagcgg cgccatcgca agcacgatga
     6001 agctgtaggg aatgacgacg agcagcagta tcagctggta aagcgtcgag cgcaggaggt
     6061 tcatggcgat ccgagcaatg cgtctgccac ggcggcgaga tcgggaaaga tgcgcgtgcc
     6121 cggcggcagg ttttcatgtt gggtgctgac gcccttgccg gtgagcacca gtatgggttg
     6181 cgccttgacg gccgccgcag cttggagatc gcgcagcgcg tcgccgacgg cgggcacgtc
     6241 ttcgagcggc atgttcaggc gctcgccgat ttccaccagc atgccgggat tgggcttgcg
     6301 ataaggcgaa ttggaatccg ccccggcggg gcaatagaaa atcgcgtcga tgcggccgcc
     6361 ggcatgggcg acctggcggt gcatcttgtc gtgtatcgcg ttgagcgtcg ccatatcgaa
     6421 caggccgcga ctgatgccgg attgattggt cgccaccact acgcgataac ccgcctgcgt
     6481 gaaacgcgcg atggcctcca cgctaccggg tatgggtatc cattcgtccg ggcttttgat
     6541 gaactgcgga ctgtcgtgat tgatgacgcc gtcgcggtcg agaatgatta gcttcatgca
     6601 gcgtggtgta cggcacggca gggcgtcatt ctaccttgcg acgggcggtt caccgtcatg
     6661 ctgcaagtcg cgagatatcg gccacgcgat tcatcaggtc ttccaatgcg ccgagcaagg
     6721 ccaggcgatt ggcgcgcacc gcggcatcgt cggtcatcac catgacttcg tcgaaaaaac
     6781 ggtcgaccgc attgcgtgcg ccggccagcg cgctcagtgc ttcggtataa cgccgtgcct
     6841 gcatgtgcgc atcgatgccc ggtgccagac tctgcagcgt gtcgtgcagc gcctgctcgg
     6901 ccggttcctg cagcaattcc ggctgcggtg cggcggcatg cggatccgtc gccttcttca
     6961 ggatgttgtg gatgcgcttg ttcgctgccg cgaggctctc ggcttccggc agttggcgga
     7021 aggcgcgcac cgccgccagt cgcggcacga tggtatcgag acgggagggc gacgaggcga
     7081 tgaccgcttc gatttcgtcg gccgcgaaat cgcggtcgcg caaataggag cgcaggcggt
     7141 cgagcaggaa ggcatggacg tccgcgaccg tggacgcggc aatgacgccg ggcgcgaact
     7201 gccgctcggc cagttccagc agttcgagca catccagcgg caacgcgtgc tccaccagaa
     7261 tgcgcgccac gcccagggcg gcgcggcgca gcgcgaacgg atccttgtcg cccgtgggcg
     7321 caagaccgat gccccagatc ccgaccagcg tatccagctt gtcgcccagc gcgacggcat
     7381 cgccgatcgc gtcaccggga agttcgtcgc cggcgaagcg cggacggtaa tgcgcttcga
     7441 tggcattggc gaccgcttcc gcctcattgt cgtggcgcgc gtagtagcgg cccatgatcc
     7501 cctgcagttc ggggaactcg ccgaccatgc cggtcagcag gtcggctttt gccagatgtg
     7561 cggcacgcgt ggccagcgcc tgatctgcgt cgagctggcc ggcgatcgcg ctcgccagcg
     7621 attcgatgcg ctcgacgcgc tgcaactggc tgcccagctt gttgtggtac acgacgtcgg
     7681 cgagcttcgg caccagcgtc gccagcggct gcttgcgatc ctggtcgtag aagaactgcg
     7741 cgtccgccag tcgtgcgcgc aacacgcgtt cgttgccgcg cacgatctcg cccggatcgg
     7801 ccgcctccat gttcgatacg agcaggaagc gattcagcag tcgcccgtcc ttgccgatca
     7861 gcgggaaata cttctggttc tgccgcatgg tcagcatcag gcattcctgc ggcacttcga
     7921 gaaaggctgc gtcgaagcgg ccttcgtaca ccaccggcag ctccaccagc gcggtcactt
     7981 cggcgtagag cgcctcatcc gccgcaatga ccgcctcacc cgcggcttcc tgcagcgatt
     8041 cgcgaatcag cgccaagcgg cgctcgaagc aggcgatcac cttgccatcg cgttcgagcg
     8101 tggattcgta agtgtcggcc gatgcgatat ccagcggtcg cgaagcgaga aaacgatggc
     8161 cgcaggtacg gcgccccgcc tgcaggccga agagggaagt gggcaccacg gtttcgccgt
     8221 gcagggcgac cagaccccgc acggggcgga cgaactccgc atcgcccgcg ccccaccgca
     8281 tcattttcgg aatgggcagg cgcttcagcg ccgcggcgat gatctcctgc agtacatctg
     8341 cgagacggct gccgcgttcc gtcttgcgat aggcgaacac ttcgttcttg ccgtcctgca
     8401 ggcgttccag gacctcgaca tcgacgccct gtttgcgcgc gaagccgagc agcgcggggc
     8461 tgggcttgcc cgcggcgtcg aggcctacct tgagcgacgg gcccttgacc agcacttcgc
     8521 ggtcgggtgc ctgggcggac acgtcctgca ccagtacggc aagacgacgg ggcgaggcga
     8581 aagtccggtg cgcggcggcc gcgtcgatga aggcgtgcgc gcgcaggtcg gcgaccagtg
     8641 cggtggcaaa gctgtcgccg agttggcgca gcgccttggg cggcaattcc tcggtgagca
     8701 attcgatcag cagggcagcc ttagtcatgc gccgtcctcc gcaccaaggg gaagccgagc
     8761 cgttcgcgcg actcgtaata agcctgtgcg acctgacgtg cgagggcgcg cacccgtccg
     8821 atatacgcgg cgcgctcggt caccgagatc gcgccgcgcg cgtcgagcag attgaaggta
     8881 tgcgaacact tcatcaccat ttcataacct ggcaggggca ggccggcggc aatcaatcgc
     8941 tttgcctcgc tttcgaactc tccgaacagt tcgagcagcc agggcgcatt cgcctcttcg
     9001 aagttgtaac gcgactgctc cacctcgttc tggtggtaga cgtcgccgta agtcacggag
     9061 ccatccgggc cttccgccca gaccaagtcg tagacgttct ctttgccctg cagatgcatg
     9121 gcgagacgct cgagcccgta agtgatttcg cccagtacag gcttgcacgg cagcgaaccg
     9181 atttcctgga aataggtgaa ttgggtgact tccatgccgt cgagccagac ctcccagccg
     9241 agcccccagg cgccgagcgt gggcgattcc cagtcgtctt caacgaagcg cacgtcgtgt
     9301 ttcgaaaggt ccagaccgat ggcgcgcaga ctatccagat aaagatcctg gatgtcgtcg
     9361 ggcgaaggct tcagcaccac ctggaattga tagtagtgct gcaggcgatt ggggttgtcg
     9421 ccgtagcggc cgtccttcgg gcgccgggaa ggctgcacgt atgcggcatt ccagggctcg
     9481 gggccgattg cgcgcaggaa ggttgcagtg tggaaggtgc ccgcgccgac ctcgagatca
     9541 taaggctgca gcagcgtgca gccccgctgg gcccagtagc cttgcagggc gaggatcaat
     9601 tcttggaaag tgtgcatgat cggcggcagc gtcggtcgcg ccgcaaaagc aaaggcgtaa
     9661 ttctacttgg gaatatccgg gagagtgccg gggatgccgt acgcttgcgc gtacaaggct
     9721 cggaagaggg ccggactact tgcgcgcttc acgtccgaag gcgacgtgaa gcgcagattt
     9781 cgcgttatgc gggatgaatc aggactccag ttggcgctcg agtgccgcga gtcgatcctt
     9841 caaccgcagt cggcgtaatt tcaattcctg gaggcgcccg tcaccgtaga cttgcgtggt
     9901 gcataggcga ttgacgaggg tgtcgagttg tcggtgttct gctttgagag agttgatgtg
     9961 gtgatgcaat gtatcgactt ccgcatccgt catgatccgc tcctttttca ggttgaaccg
    10021 gcgataaaag tgtaacccgg cgcccggatt gcgggcgtac ggtcgatgcc ggtatgcggg
    10081 agtgcgctgc gctaggcgcg acggcggtgc agaacgccca ggccgagcgc gccgagcgcc
    10141 cagatcagcg cgggccagtt gccgaaacgc acgtaaggcg tggcgccctc gcggccctgc
    10201 gcgctgccga gcagtgcgcc ttcggtgaat ggcggcaggc gcctttgtac cgtcccgtcc
    10261 gcctcgatga tggcggtaat gcctgtattg gtggcgcgca gcatggtgcg accggtctcc
    10321 agggcgcgca tccgggcgat gcgcaaatgc tgctccggcg cgagcgagtc gccgaaccac
    10381 gcgacgttgc tcacgttcac cagcagcgtc gcttcgggca actggcggat gatttcctcg
    10441 ccgaacgcgt cctcgtagca gatgttcaac gccacccgtt cgcccgcgac atgcaacggc
    10501 cgttgggtgg tcgcgccggc ggaaaaatcc gagagcggga tctgcagcac attcagcacc
    10561 cagccgaagc cgggcggaat gaactcgccg aagggcacca gatgcacttt ggcgtagcgc
    10621 tgcatcggcg cggtacccag gctcaccgcc gcgttgtagt agccgccgtt cgcatcgcgc
    10681 aggggtgcgg gcaccagcat gtcgcccgac tgcacgcgcg cgatgtcggc cagttgttcc
    10741 agataagccg gatcgacctg atcgagaaaa cgcggaatcg ccgtttccgg caggacgatc
    10801 agctgcgccg gtgccgtgcg ggctagacgc gcgtacgtgt gcagcgtctg ctcgtagcgc
    10861 cccggtacga acttcagttc ctgcgggata ttcccctgca gcaggctgac ggaaatgggc
    10921 gcgcccacgg gccgggtcca tgtcacgctt cgcagcgtga gtcctgcacc gatcaatgca
    10981 acgaagaccg cggccccgat tatcctttcg tgtccgcgct gcatgaacgc aacgagcgcc
    11041 cccatggcga tggccgccag cagcgacaag ccgtagacgc cgaccagcgg cgcataagcg
    11101 gccagcggcg tgtccaccgt gctgtaaccg aaggcgagcc aaggaaagcc cgtcaacagc
    11161 cagccgcgca gccattcggt gagtacccag caggccggca tgtagagcat cagccgcagg
    11221 gcgggggagc cgggcaagtg cgcctgcaga tagccggccg cagcgggaaa cagcgccaga
    11281 taaaggcaga aaagcagtgt ggcgacggct gccagcggcg cgggcatcat gccgaagtca
    11341 tgcaggctta catagaccca gctgacgccc accagaaaag cgccgacgcc ccaggcgtag
    11401 cccagtccgg cggcctgtcg cggcgtctcg gccgtatgcc acagccggag cagcgcggcg
    11461 aatgcaagca gggatagcgg ccacagcggc agatcgccga agccggcgac cgataacgcg
    11521 ccggcggcca ccgcaacgag cgtcttgcga cgcacgggtg ggatcaagac gtctcctgcg
    11581 cgcgttcgac caggagggta tgcacgcgcc ggctgtcggc gcgctgcacc aggaatctga
    11641 agccgtcgat ggtgatgctt tcgccgcgct tgggcaggcg gccgaaacgg ctgatgacca
    11701 tgccgccgac ggtatcgtgt tcctcgtcgg agaagtctgt ggcgaactgt tcgttgaagt
    11761 cggcgatctc cgtctgcgcc ttcacccgcc agcggccgct gcgttcgcgc atgatgttgt
    11821 cctggatctc gtcgaaatcg tgttcgtcct cgatgtcgcc gacgatctgt tcgagcacgt
    11881 cttcgatggt gaccaggccg gcgacgccgc cgtactcgtc caccacgatc gccatgtgat
    11941 tgcggctgac gcggaactcg cgcagcagga tgttcagccg cttggattca ggcacgaaca
    12001 ccgccgggcg cagcatgtcg cgcacgttga attcgtcgtc gccggcgtaa tagcgcagca
    12061 gatccttggc cagcaggatg ccgatgacgt ggtcgcggtc gcggtcgatg accggaaagc
    12121 gcgaatgcgc cgtggcaatg acttgtggaa tgaactcctc gggcgagtcg ttgatgtcga
    12181 ttacgcacat ctggctgcgc ggaatcatca cgtcgctcac cttcacttcg gagacctgca
    12241 gcgcgccttc gatcatggtc agggcgtcgg catcgagcag gttccgttcg tgcgcggaat
    12301 gcaatagcgc gatcaactga ttgcggtcct cgggttcgcg catgatcagc gcgccgaggc
    12361 gttcgagcag gctgggtttg tgtgtctggt cttcgtccat atgcaaatct acggcatccg
    12421 taccggctta gccggcgggg tcgtagggat tgtcgaaacc gagttcgcgc agcacggcga
    12481 tttcgcggcg ctccatgtgc tgcgcttcgc gcgttcgttc gtgatcataa ccctgcaggt
    12541 gcagcatgcc gtgcaccacc aggtgcgcat agtgcccacg cagcgccttg cgctgctcgc
    12601 gcgcttcgcg ggcaaccacc ggcgcgcaca ggactatatc cccgagcagg gcaccttcat
    12661 gctgtccata agtgaaagtc agcacgttcg ttgcgtagtc gcggccgcga tattggcgat
    12721 tcagcgcgcg tccttcgcgc gtgccgacaa agcggaccgt tgcctgcgcg gactgttcca
    12781 gcgcggcgag cgcccagcgg cgtacgctgc gcgccgccgg caatccgctg cgcggcacgg
    12841 cgtactgcac ttgcattgca agttgcggcg gtcgcctgcc cactagtcgc ggcgcggcgg
    12901 gcgcagtccg gcgcccactt cgtaatgttc ataggcgttg acgatccgcg ccaccagcgg
    12961 gtggcgcacg acgtcttccg attcgaacac cgtgaaagcg atgccgcgca catccgctag
    13021 gacatgccgc gcttccacga ggccgctttt ctggcccttg cccaggtcga tctgcgtcac
    13081 gtcgccggtg atcacggcct tggtgccgaa gccgatccgc gtcaggaaca tcttcatctg
    13141 ttccggggtg gtgttctgcg cctcgtcgag gatgatgaag gagtgattga gagtgcggcc
    13201 gcgcatatag gcgaggggcg cgatttcgat cgcgccgcgc tcgaacatct tgcccacctt
    13261 gtcgaagccc atcagatcgt agagcgcgtc gtacagcggg cgcagatagg ggtcgacctt
    13321 ctgcgcgaga tcgcccggca gaaagcccag acgctcgccc gcctcgacgg cgggacgcac
    13381 gagtacgatg cgcttcaccg cgtcgcgctc aagcgcgtcc acggcgcagg ccacggccag
    13441 ataggtcttg cccgttccgg cgggccccac gccgaaggtg atgtcgtttt cgacgatgtt
    13501 ctgcagatac tgccgctgcc gcggcgtgcg gccgtgcaag tcgggtcggc gcgtcagcag
    13561 cgctggcccc tgcttctcgg gcgcgaccgg ctgcatggct tcgacgatgc ccagttgcac
    13621 gtcgtcgatg gacaactctc gcgtcgcgcc ggcgtagaac ttgcgcagca tgtcggccgc
    13681 gcgctgcgcc tgttcgggtg tgccgcgcac actgaagcga tcaccgcggc gcgcgatgga
    13741 gacgtccaaa cctgcctcca tctgtcgcag attctcgtcc agcgcgccgc acagatgggc
    13801 caggcgctgg ttatccgcgg gctccagcgt caattcgagc gtcttgctgc gctgggcggt
    13861 ctttgccggg gctttacggg cgttcaagtc gcaatggcga gggcgtcctc gcgcaagtgc
    13921 atgcgtccgc gcagcgagtg cgcataggcg gtttcgattt ggacgtccac gaaccggccg
    13981 atcaaccgtt cgggcgcggg gaaattgacg acgcgattgt tgtcggtgcg gccggccaat
    14041 tcgttttcat ccttcttcgc ccggccttcg acgaggacgc gctgaaccgt cccgaccatc
    14101 gcctcgttga cggcgcggcc gttggcctcc agcttggcct gcaagcgcat cagccgctcg
    14161 actttcacct catgcggcgt gtcgtcccgt aattcggcgg ccggcgtgcc gggacgcgcg
    14221 ctgtagatga acgaaaagga ggcgtcgaag cccacatcgt ccaccaactt catggtctgt
    14281 tcgaaatccc gctccgtctc gccggggaag ccgacgatga agtccgagga aatgcagata
    14341 tcgggtcgcg cggcgcgcag acggcgaacg atcgatttga actccagcac cgtgtaaccg
    14401 cgcttcatcg ccgccagcac gcggtcggag cccgcctgca cgggcaagtg cacatgcgag
    14461 acgagcttcg gcagcttggc gtacgcgtcg atcagtcgct gggtgaattc cttgggatgc
    14521 gaggtcgtat agcggatgcg ctcgatgccc ggcacgtcgg ctacgtattc gagcagcatg
    14581 gcgaagtcgg cgatctcgcc gtccggcagc cggccgcgat aagcgttgac gttctggccg
    14641 agcagcgtca cttccttcac gccgcggtcg gccagatcgg cgatttcgac gattacgtcc
    14701 tcgaaggggc gcgacacttc ctcgccgcgc gtatagggca cgacgcaata gctgcaatac
    14761 ttgctgcagc cttccatgat ggagacgaaa gccgtcgccg cttccacgcg cgcgggcggc
    14821 agatgatcga acttctcgat ttccgggaag gaaatatcca cctgcggttt gccgctgcgg
    14881 cgacgctcgg cgatgagttc gggcaggcga tgcagggtct gcgggccgaa caccacatcg
    14941 acgaagggcg cgcgtttgac gatggcttcg ccttcctggc tggccacaca gccgcccacg
    15001 ccgatcacca aatcggggtt ctgctgcttc agcgcccgca cgcggcccag gtcgtgaaac
    15061 accttctcct gtgccttctc gcgtaccgaa caggtattga acagaatgac gtccgcctct
    15121 tcggggcggt cggtcggctc caggccttcc gcggcgtgta acacgtctgc catcttgtcc
    15181 gagtcgtact cgttcatctg gcagccgaag gtcttgatgt aaacgcgttt actcatcgct
    15241 actgatcgtc cagcccgtcg ccccggggct cgtccttgtt ttcctgtatc ggcgcgggca
    15301 ggggcaccgg cttctgcgcc tcgacctgcg ccgcctcggc ggcgctgagc agccagaccc
    15361 gcttgatctg ccccatgttg tcgagctgat agcgtacggg cgcccgcgtc ggcaccatgg
    15421 tgggcagcac gatgatattg ctcgtgtcgc gaatgagcgc gccgggcgcc agcaggtact
    15481 gtttatcgtc cagttgcacc acgttctgct gcaggacggt catcctggca ggtttcgcat
    15541 cggctggaat ctggcggaat tgcgcccacg cggcgctgct cagcgcggcg cccagcacgg
    15601 cggccagcag ggatcgtaag aggaaaagac gcatgcagag gtccgggaag ggaaggcggc
    15661 tattctaggt aaatacctgc tgacacggcc aggaaagccg gcgggaaaag cggaaacggg
    15721 acgcgattgc cgactcgagc gatgtgcgcg ttttttgctt gaggcgtttg caaaaagcgg
    15781 gcattccgac tcggccgaac ggcgctcgcg ccgccggcct taagaatgcg aaagtggtgg
    15841 cgaatcaggg atttgaaccc cggaccaacg gattatgatt ccgctgctct aaccgctgag
    15901 ctaattcgcc acgcgggagc gggattatcc cgattggggc ggggaagtgt caaggttcaa
    15961 ggcggtgtgt caagttccgg ttgacagacg ccgaggcgcg cacgcatcat ccgcgcaatc
    16021 tgtcacatgg aaaaatgagg cgccaagcat tctgacggcg tcgattggag gttgagcatg
    16081 aaagtatgcg tagtcggcgc cggcgcgatc ggtggtctgg tcgcggtgag actcgccctg
    16141 ggcggccaag atgacgttac tgtcgtcgtt cgtggcgcca atctcgaagc cattcgcgcc
    16201 aacggcatga agctgatcga gcacgacggc acggagcgcg tggcgaaaaa cattcgcgcc
    16261 tgcacctggg acgatgccgg tccgcaggac gtggtgattc tgggtatgaa agcccatcag
    16321 gtcgagccca ttctgcccga tctggacaag attctgcacg aggacaccat catcatcccg
    16381 atgcagaacg ggattccgtg gtggtatttc cagggccagg gcgatgttcc gcacgccaac
    16441 cagacggtcg aaagcgtcga tccgggcggc aagatcaagt cggccatcga tccgcgccgc
    16501 atcctcggct gcgtcgtcta tccggcttcc gagctgaccg ctccgggcgt cattcatcac
    16561 atcgaaggcg accgcttccc gatcggtgaa ctgaacggcg ccatcaccga gcgcgcgcag
    16621 cgcgtgtcgg aaatgttcca gcgcggcggc ctcaaggcgc cggtgctcga gaacatccgc
    16681 tcggaaatct ggctgaagct gtggggcaac ctgacgttca acccgatcag cgccctgacg
    16741 cactcgaccc tggtcgatat ctgccaattc ccgctgacgc gcgaactggc cgcagccatg
    16801 atgaccgaag cgcaaacggt ggcgcacaag ctgaacatca ctttccgcgt gccgctggag
    16861 aagcgtatcg ccggcgccga gaaagtgggc aagcacaaga cctcgatgct gcaggatgtc
    16921 gaagccgggc gcgatccgga aatcgatgcg ctggtcggct cggtgatcga gttggcaaag
    16981 ctgaccgata cgccggtgcc gcacatcgag accaccttcg ccctcgtgaa actgctggcc
    17041 aagaccatgc aggaagagaa ggtgctcgtg cgcgcgcagc cgctcggctg aagacgcgcg
    17101 gcggtggcac ggtagaatcg acgcgtgcca ccgtccatct ccactgactt cgacgtactc
    17161 atcgctggcg ccggcctggt cggcgccagc tttgcgctgg ccctgcgcga cagcggcttg
    17221 cgcgtaggta tcatcgaacc tgcggaaccc tcgccgcccg gcgaggcatg ggacagccgt
    17281 atctatgcgc tgaatcccgc gaacatcgat ttcctgcaaa cgctcggcgc atggcagcgc
    17341 atggatcctg cgcgcctgca gccggtcgga cgcatgcaga tctacggcga cgaccaggct
    17401 cgcctggatt tctcggccta cgagtcgggt gtggctgcgc ttgcgtacat cctcgaatcc
    17461 gggcgcttgc aatacgcgct gtgggaatcg ctgcgcgatg cgccgaacat cgaactgctc
    17521 acgggcgcac gctgcgccga cgcggatttc gaatccgctg cggcgcgcat taggctggac
    17581 gacgggcgga cgctgcgcac ggagttactg gtcggcgcgg acggtgccaa ctcctgggtg
    17641 cgccgtgtgg cgcgattgca agcccaggta cacgcttatg agcatctcgg cgtggtcgcc
    17701 aatttcgatt gcgccaagcc gcacggcggc acggcgttcc agtggttccg tcacgacggc
    17761 atcctggcct atctgccgct ggccgggcag cgcatgtcga tggtgtggtc cacgccggac
    17821 gaacacggtc gcgagctgct tgcgctgccg cacgaacaac tggccgcgcg cgtcgcagaa
    17881 gcgggccggc atgtgctcgg cgaactgcga gtcatcacgc cgccgcaggc gtttcctttg
    17941 cgcaaaatgc gtttgcccga tgtcgtcgcg ccgcgcgtgg cgttgatcgg cgacgcggcg
    18001 catgtcgtgc atccgctggc cgggcagggc gtaaatctgg gtttcggcga cgcggcggcg
    18061 ctcgcacgcc tgatcatcga gcgggaaccc ttccgccgat gcggcgacat ggcgatcctg
    18121 cggcgctacc agcgggaacg cgccgaaccg gtcgctgcga tgggtgcggt cacgcacggc
    18181 ttgcagcgtc ttttcgccgc acccggttcc tgggttgctg cgttgcgcaa ccgcggtctt
    18241 gcgtacactg accgtacgac ggtcatcaaa aacctgctgg tgcgtcaggc gctcggctaa
    18301 acatcaggaa agaaacccca tgctgcgtat tctgctttgc gctttgacgc tctcgctcag
    18361 cgcccctgct ttcgccgacg aggcggcgat caagaaagtc atcgaggccc gtatgcaggg
    18421 cgccaaggtg gagaagatca ccaagacgcc ctactcgggt ctgtacgaag tgcagatcgg
    18481 caatgaggtg ctctataccg atgcgcaggc gaattacatt ttcgtcggca atgtgcacga
    18541 cggtcagacg ctggagaatc tgaccgaggc gcgtctgcag gaactgaaca aggtggactt
    18601 cgaccgcttt ccgctcgatc aggccatcaa gctcgtgaac ggcaagggca cgcgccagct
    18661 cgtgtacttc agcgacccga actgccccta ttgcaagcgc atggacaagg aactgcaggc
    18721 gatcgaggac ctgaccgtct acgtgtttct ttaccccatc ctgtcgcccg attccatggc
    18781 gaaatcgcag
//
LOCUS       DATGOU010000043         4182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3657024, whole genome shotgun sequence.
ACCESSION   DATGOU010000043 DATGOU010000000
VERSION     DATGOU010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3657024"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VKZ48_02385"
     CDS             complement(<1..527)
                     /locus_tag="VKZ48_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MSHA biogenesis protein MshI"
                     /protein_id="HLU76734.1"
                     /translation="MSQQLNLFNPVFLRQKKYFSAVTMLQCLTIVLIALIAVYGFQRW
                     QLSGLERQLADARLQHNAVQQQLTQLAAAKRTPSAALKDEAMRLEAQAGALEALLGRL
                     ESGELGNTEGFSRYLGALARQTLPGVWLTGFRATGHEGPQIIKGRMLQPELLPAYLRA
                     LNKEETLRGHGFAEL"
     gene            complement(524..1417)
                     /gene="pilM"
                     /locus_tag="VKZ48_02390"
     CDS             complement(524..1417)
                     /gene="pilM"
                     /locus_tag="VKZ48_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HLU76735.1"
                     /translation="MVIGAGNGELTAVLGDAGAGRPAVRQYAVRAVEETASGLHHAAR
                     DLGAGRYQCATLLAPDEYQIVVVDAPNVPRDELKTAIRWRVKDLIDAHIDDVTLDILD
                     IPVPKDTPARNHTMYAVAGRNDAIQAVIKRFEDADIPLTVIDIPETAQRNVAALYEEP
                     ERAVALLHFDEHEGLLTINYAGELYFARRFDFPYAQLNADDEMARDDARGRVLLELQR
                     SLDHFERQFRSIPVSKLLIAPEPFSTELADYLAAESGFPVQRIDLNDVIDFDGGSLDP
                     RLQWRLFHSIGASLRQEVKAL"
     gene            complement(1584..4103)
                     /locus_tag="VKZ48_02395"
     CDS             complement(1584..4103)
                     /locus_tag="VKZ48_02395"
                     /inference="COORDINATES: protein motif:HMM:NF042738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6701 domain-containing protein"
                     /protein_id="HLU76736.1"
                     /translation="MNNYRYHAGTNTLSPGVDVSGSTAGPGHTYRIVVDHSNSANAWV
                     SVQRKTGANFETLIERYDAKAQTGQAAVPEKWILSWTGSTGGSTNVHEIDNLRICATN
                     METIASADHYAIQHATSGVNCQPENVTITAKNASNAAVSANGATITVTARRVAGTPAA
                     NRGDWILVAGSGALDNGTADDGVATYTFGPDEQQVTLALKNTHLQTVNIDVDGQGVTE
                     GSGAGASEPGYNQNLTFAPSGFRFTNGANPPSPFSIPTQTAGVTSATYALQAIRTDTN
                     TGACVGAFADNADVAVDLAFQCNNPATCQSGQLVSITNNGSTSTIAANPGSGVSNYSA
                     RSLRFGPNSQALFTLVYPDVGQITLHASHNIPLANGSPSDNLMLGSSNAFVVKPYDFR
                     VSDIKTTAGGAENPEAEDAGGPIFVRAGESFSATVTSVNAQGDATPNYGKESTPEGVK
                     LTSALVGGLGLVANPALANADAFSTFDAGSATGTAFSWNEVGIVRLTAEVADGNYLGT
                     GNVVGTPSANVGRFIPDHFKLSGGALKNRSDAGCTPDSAFTYMDEPMLLKYSLAASNT
                     SGGTTQNYQGAFAKLNVGSAAAHGFGVRDTASSSTLTSRLDVLSVTGNWSGGVADIDA
                     AVSIARSSAPDGPYNALRIGIAPVDGDGVRLATATLDLDVDGNSSNDHAQIGETTRIR
                     FGRLRLFNALGSERLPLAMPMQLEYWNGTAFVVNSDDNCTTLARSTIAMAPDSGLAPC
                     KTAFNEASIAFNGGKATPVLAAPTPAHGSVDLRVNLGSGSGDHCPAVNGSATAATSAI
                     RSYLQGRWNGADYDDDPVARATFGVYGAQVPKRFIYSRENY"
BASE COUNT          702 a         1287 c         1371 g          822 t
ORIGIN      
        1 tgcagttcgg caaagccgtg gccgcgcagc gtctcctcct tgttgagggc acgcaggtag
       61 gcgggcagga gttcgggctg cagcatgcgg cctttgatga tctgcggacc ttcatggccc
      121 gttgcgcgga agccggtcag ccacacgccg ggcagcgtct gccgggcaag cgcaccgagg
      181 taacgcgaga agccctcggt gttcccgagt tcgccgcttt ccaggcgacc gagcagggct
      241 tccagcgcgc cagcctgggc ttcgaggcgc atcgcctcat ctttcagggc ggcgctcggc
      301 gtgcgcttgg cggccgcgag ttgggtgagt tgctgctgaa ccgcgttgtg ctgcaacctg
      361 gcatccgcca gttgccgttc cagcccgctc agttgccagc gctggaagcc gtacacggcg
      421 atgagcgcga tcagcacgat ggtcaggcat tgcagcatcg tcaccgcgga gaagtacttc
      481 ttctgccgca ggaagacggg gttgaagagg ttgagttgct ggctcacagc gctttcacct
      541 cctggcgcag cgaagcgccg atggaatgaa agagccgcca ttgcaggcgc gggtccaacg
      601 acccgccgtc gaagtcgatg acgtcgttga gatcgatgcg ctgcaccgga aatccgcttt
      661 ccgccgccag gtagtcggcg agctccgtcg agaacggttc cggggcgatc agcagcttgg
      721 acacgggaat ggaccggaac tggcgctcga agtgatccag cgagcgctgc agttcgagca
      781 acacgcggcc gcgtgcatcg tcgcgcgcca tctcgtcgtc ggcgttcaac tgcgcgtatg
      841 gaaaatcgaa gcgccgggcg aaatacaact cgccggcgta attgatggtg agcagccctt
      901 cgtgctcgtc aaagtggagc agggcgacgg cgcgctcagg ctcttcgtac agcgcagcga
      961 cattgcgctg cgccgtctcg ggaatgtcga tgaccgtcag cggaatgtcg gcatcctcga
     1021 agcgcttgat gaccgcctgg atcgcgtcgt tgcggccggc gaccgcgtac atcgtatggt
     1081 tgcgcgcagg cgtgtccttc ggcacgggaa tgtcgagaat gtcgagcgtg acgtcgtcga
     1141 tatgcgcgtc gatgagatct ttcacgcgcc agcggatggc ggtcttcaat tcatcgcgcg
     1201 gcacgttggg cgcatcgacg accacgatct ggtattcgtc cggcgcgagc agcgtcgcgc
     1261 attgataacg gccggcgccc aggtcgcgcg cggcatggtg caggccgctc gccgtctctt
     1321 cgacggcgcg gacggcatac tgccgcaccg cagggcggcc agcgcccgcg tcgccgagca
     1381 ccgccgtcag ctccccgttc ccggcgccaa tcaccatcca gcccgactcg cgccgcgctt
     1441 tacgccaccc aaacacacgt tcccccattt ttcttatcgg ggcgatattg ccgcccccaa
     1501 cttccccgaa taagccagct tccccgctta agcaattgtc gtacaaggac tgtttgcgct
     1561 cattttttac tgtcgaataa gcgtcaatag ttctcgcgcg agtagatgaa ccgcttaggg
     1621 acctgcgccc cgtacacgcc gaaagtggcc cgcgccaccg ggtcgtcgtc atagtcggcg
     1681 ccattccacc gcccctgcaa atagcttcgg atggcgctcg ttgccgcggt tgcggaaccg
     1741 ttgacggccg gacagtggtc gcccgacccg ctgcccagat tcacgcgcag gtcgacgctg
     1801 ccgtgcgcgg gtgtcggtgc tgccaacaca ggtgtcgctt taccgccgtt gaacgcgatg
     1861 ctcgcttcgt tgaatgcggt cttgcagggc gcgaggccgg aatcgggcgc catggcgatc
     1921 gtcgagcggg cgagcgtggt gcagttgtcg tcactgttca cgacgaaggc ggtgccattc
     1981 cagtactcca gttgcatcgg catggcgagc ggcagccgtt ccgagccgag cgcgttgaag
     2041 aggcgcaggc ggccgaagcg gatccgagtc gtctccccaa tctgtgcatg gtcgttcgac
     2101 gagttgccgt ccacgtccag atcgagcgtg gcagtcgcca agcgcacgcc gtcgccatcc
     2161 accggcgcga taccgatgcg cagcgcgttg tacgggccat cgggcgcgct actacgtgca
     2221 atggaaacgg ctgcatcgat atcggccacg ccgccgctcc agttgccggt cacgctgagc
     2281 acgtcaagcc ggctcgtcag cgtgctgctg ctggccgtat cacgaacgcc gaaaccgtgt
     2341 gcggcggcgg aacccacgtt tagcttggcg aaagcgccct gataattctg cgtcgtgcct
     2401 ccgctcgtgt tcgaggcggc gagggagtat ttcagcaaca tcggctcgtc catgtaggtg
     2461 aaggccgaat cgggcgtgca gcccgcgtcg gaacggtttt tcaacgcgcc gcctgacagc
     2521 ttgaagtgat cgggaatgaa gcggccgacg ttcgcgctcg gcgtgccgac cacgttgcct
     2581 gtgccaagat aattcccgtc ggccacctcg gcggtgagcc gaacgatgcc gacttcgttc
     2641 caactgaagg ccgtgccggt ggccgaaccc gcatcgaaag tgctgaaggc gtctgcattg
     2701 gcaagtgccg gattggctac gagcccaagg ccgccgacca gtgcgctggt gagtttgacg
     2761 ccttcgggcg tgctctcctt gccgtaattg ggcgtcgcat cgccttgcgc gttgaccgag
     2821 gtcacggttg cggagaacga ttcgccggcc cggacgaaga tcggcccacc ggcatcctcc
     2881 gcttcaggat tttccgcgcc acccgccgtc gtcttgatgt ccgaaacccg gaaatcgtag
     2941 ggtttcacga cgaaggcatt gctcgacccg agcatcaggt tgtcggacgg gctgccgttc
     3001 gccagcggaa tattgtgact ggcatgcagc gtgatctggc cgacatccgg atagaccagc
     3061 gtaaacagcg cttgcgaatt tggaccgaag cgcagactgc gggcgctgta gttcgaaacg
     3121 ccgctgccag gattggcggc gatcgtgctc gtcgagccat tgttggtaat gctcacgagt
     3181 tgcccgctct ggcaggtcgc ggggttgttg cattggaagg cgagatcgac cgctacatcc
     3241 gcgttgtcgg caaacgcacc gacgcacgca ccggtattcg tgtcggtccg gatggcctgc
     3301 aacgcataag tcgcggaggt tacgccggcc gtctgcgtgg ggatcgagaa gggcgagggc
     3361 gggtttgcac cgttggtgaa gcgaaaaccg cttggagcga aagtgaggtt ctggttatag
     3421 ccgggttcgc ttgcgccggc gccgctgcct tcggtgacgc cctggccgtc cacgtcgatg
     3481 ttcacggttt gcaggtgcgt gttcttgagg gcgagtgtga cctgctgttc gtcgggaccg
     3541 aaggtgtagg tcgccacacc atcgtcggca gtgccattgt caagcgcacc ggagcctgcg
     3601 acaagtatcc agtcgccgcg attggctgcc ggtgtgccgg cgacgcgacg tgcggttacg
     3661 gtgatcgtcg caccattcgc ggagaccgcc gcgttggaag cattcttcgc cgtgatggtc
     3721 acgttctccg gctggcagtt gacgccgctg gtcgcatgtt gaatcgcgta gtgatccgct
     3781 gacgcgatgg tctccatgtt cgtcgcgcag attctcagat tgtcgatttc gtgaacgttg
     3841 gtgctgccac cggtcgagcc ggtccaggat aggatccact tttccggcac ggcagcttgt
     3901 ccggtttgcg ctttcgcatc gtagcgttcg atcagcgtct cgaagttggc gccggttttg
     3961 cgttgcacgg acacccatgc gtttgcggag ttggaatgat cgacgacgat gcggtacgta
     4021 tgccccgggc ccgctgtcga gccgcttaca tcgacgccgg ggctcaatgt gttggtgccg
     4081 gcatggtagc gatagttatt catgcccgaa cccgagccgc ggacggaaac cgaatccgca
     4141 cgctgacccg gtccgcctgt tttgcccaac tcggttgacg ag
//
LOCUS       DATGOU010000044        11518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_992125, whole genome shotgun sequence.
ACCESSION   DATGOU010000044 DATGOU010000000
VERSION     DATGOU010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11518
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992125"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="VKZ48_02400"
     CDS             complement(<1..1064)
                     /locus_tag="VKZ48_02400"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HLU76737.1"
                     /translation="MSKAIYIVDGARTPFLKSRNKPGPFAASDLATIAGRALLMRQPF
                     EPFQLDEVILGCAVPAPDEVNIARVSALRMGCGQHVPAWTVMRNCASGMQSLDSAIAN
                     MMSGRSDLVLAGGVDALSRAPMLLSEGMVMWLSNFYAAKSVGQKAALLAKFRPQYLAP
                     VLGIMKGLTDPNVGLIMGQTAENLAYRFGITRTQQDEYAARSHQRVAQAQENGWFDEI
                     VPMYDDRGNAYTRDDGVRADSTVENLAKLKPFFDRQYGTVTAGNSSQITDGAVWTILA
                     TEEALAQYGLTPIGRIVDTHWAALDPAHMGLGPVHAATPLLKAYDLGLDDIDAWEINE
                     AFAAQVLGCIEAWKDDAYCR"
     gene            complement(1077..3398)
                     /locus_tag="VKZ48_02405"
     CDS             complement(1077..3398)
                     /locus_tag="VKZ48_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015039380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HLU76738.1"
                     /translation="MIALYTLLVLVTVAVVIFGAPPIRRVLLSNRILAVFRKVMPPMS
                     QTEADAINAGTVWWDGELFSGKPDWERLLGYPKPTLTPEEQSFLDNECEELCRLCNDW
                     DTYAINDLPPEAWQYIKDKRFFGMIIPKEYGGLGFSAYMHSQVITKLSTRCSALAVTV
                     MVPNSLGPAELLLHYGTEEQKRYYLPRLAAGEEIPCFALTSPHAGSDAAAIPDRGVVC
                     KGMWNGEEVLGMRVTWDKRYITLGPIATLLGLAFRLYDPDGLLGAEKDLGITCALVPT
                     DTPGVHIGRRHFPLNAVFMNGPNWGEDVFMPLDWIIGGPKMAGQGWRMLMECLAAGRS
                     ISLPSSNTGMAKLAVRGTGAYARVRQQFNLPIGKFEGVEEPLARMGGNLYMMDAARRM
                     TAGALDLGEKPSVISGIVKYHVTERARDVLNDAMDIQGGKGICLGPNNFTGRAYQQIP
                     VSITVEGANILTRSLIIFGQGAIRCHPYVLKELTAARQNDARAFDQALWRHISFTLCN
                     GARAFFMGLTGSHFVRVPANVAPENKRYYQQLTRFSAAFAFVADISMAVMGGELKRRE
                     KLSARLGDILSLLYLCSATLKRYEDEGRNAVDKPLLDWAIWDAMFKAQSAFEGVMSNY
                     PNRVAAHILRWFVFPLGRPYVVASDRLGHKVAELLIEPSSTRDRLTADMYIPREDGEI
                     IGTLERALHAAIAAEAIEAKVRRAVKAGRIEARSPEETAQAAVDAGIISAEEQTQIAR
                     AAQLRNEVIRVDDFPPDLSLTDHARSDAARAVA"
     gene            complement(3407..4132)
                     /locus_tag="VKZ48_02410"
     CDS             complement(3407..4132)
                     /locus_tag="VKZ48_02410"
                     /inference="COORDINATES: protein motif:HMM:NF037008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HLU76739.1"
                     /translation="MAPKTLSSADRTRERIIQAAERLFLEHGFDGTSLRLVTSLAGVN
                     LGAVNYHFGGKDALFEAMLARRFDSLHAERLALLDAYQAEGRTPSCEQLLTALFKPAL
                     ARAREADADCDFLRLLGRAYIDPSPVLRKLLAERYAPTIARFKEAFAAALPQLSRQEL
                     SWRLHFMMGALAYTLAGTDAWKLIAAIQPDAGRDDEMLVRRLTPFLLAGLQAPAPLVD
                     EEPSLTPPAPYRSPTGLSPNASS"
     gene            4314..5156
                     /gene="ada"
                     /locus_tag="VKZ48_02415"
     CDS             4314..5156
                     /gene="ada"
                     /locus_tag="VKZ48_02415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019718319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HLU76740.1"
                     /translation="MNISTPSRIAARIIAACRSIEASATTPDLAALAAQAELSPSHFH
                     RQFKRLTGLTPREYARAHREARVRAALAQGARVTDAMYEAGYNSNGRFYEAADAALGM
                     KPGAYRAGGANATIRFAVGECSLGSILVAQSGRGICAILLGEDPEALLRDLERRFPRA
                     ELIGGDADFEQLVARVVGFVEAPRTGLDLPLDIRGTAFQQRVWQALRAIPPGTTVSYA
                     ELARRIGAPKAVRAVGAACGANALAVAIPCHRVVRSDGDLCGYRWGIERKRALLDIEA
                     AEKD"
     gene            5409..7253
                     /locus_tag="VKZ48_02420"
     CDS             5409..7253
                     /locus_tag="VKZ48_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HLU76741.1"
                     /translation="MNPHASLPATDAVASPPAPPRNEWRVIRLLWPYLREYRGRVALA
                     LIFLTAAKLANVGVPLLLKEIVNALDSDTAVLVMPLALLVAYGALRFSTTLFAELRDL
                     VFIRVTQRAIRRVGLDVFRHLHSLSLRFHLERQTGGVSRDIERGTKGISTLLSYLLFS
                     IIPVILEFGLVAAVLLAQFDWRFAAITFIAVGLYMVFTVLVTEWRIAIRRRANELDSQ
                     ANTRAVDSLINYETVKYFNNEEFEARRYDRNLQGYEAAAVKNEASLGLLNIGQSFIIA
                     IALTLLMILAADGVVQEKLTLGDLVLINGLLIQLYIPLNFLGMVYREIKQATVDMDRM
                     FGLLDEHREVEDRPDAKPLPAGALSVRFERVNFSYEPKRQILYEVDFDIPAGSKVAVV
                     GHSGSGKSTLGRLLYRFYDVTAGRITVNGIDVRDLKQSDLRGAIGIVPQDTVLFNDTI
                     GYNIRYGRPEASDAEVIEAAQAAHIHDFIAALPDGYETRVGERGLKLSGGEKQRVAIA
                     RALLKRPRILIFDEATSALDSKSEQAIQDELNRLARGHTTLVIAHRLSTIMDADEIIV
                     LDRGRIVERGGHRFLLNQGGPYAQMWALQQQEREADELAGAAAGGAET"
     gene            7418..8347
                     /gene="pbpG"
                     /locus_tag="VKZ48_02425"
     CDS             7418..8347
                     /gene="pbpG"
                     /locus_tag="VKZ48_02425"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF008668.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HLU76742.1"
                     /translation="MKSVWAQTAVAVVFAFTLLSGAYAASAPSEPQIRSAAVLVMDQE
                     TGEVLLSQNSGDVVPIASLTKLMTALVTLEAGLDLDQKVRITNDDIDTLKGTGSRLRV
                     GSVFTRDELLKLALMSSENRAASALGRSYPGGVSAFVNAMNAKASEIGMLHTRFVEPT
                     GLSSANVSSAEDLAKLVRAAHGVPLIREYSTMPRHTVRIKGRPAQFVNTNALVRAGQW
                     DIGLSKTGFISEAGRCLVMQAKFNARSVIIVLLDSFGKYTRVADAKRIRAWLDPDFTL
                     KSQQIVRSKAVKVKAKRNKSSNRVRTQIKAAAR"
     gene            8349..9344
                     /gene="egtD"
                     /locus_tag="VKZ48_02430"
     CDS             8349..9344
                     /gene="egtD"
                     /locus_tag="VKZ48_02430"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HLU76743.1"
                     /translation="MAGTAPAGDARRPDPLCLHDLRGPQTGFLDDVLAGLAQLQKRIP
                     PKYFYDADGSHLFEAICALPEYYPTRAETALMDAHAGDMATRLGAAIQLIEFGSGAST
                     KTQRLIDAARPSRYVPIDISESALREACSRLRAAYPHLLIHAVCADFNAPLQLRAPIL
                     ADDRPRVVYFPGSTIGNFTRAEARAFLQRVAALLGPGGRLLIGVDLKKDRAILEAAYN
                     DAQGVTARFNLNLLERMNRELGADFRIERFRHRAFYNAEQSRIEMHLDSRIAQTVRIA
                     GREFSFRAGESIHTEISCKYGLAEFQDLGRAAGFDPLAVWTDADGLFSVHLMQAA"
     gene            9389..9958
                     /locus_tag="VKZ48_02435"
     CDS             9389..9958
                     /locus_tag="VKZ48_02435"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HLU76744.1"
                     /translation="MAKHFFRRVLPDPEWAARHRFLARLGPRITAPQLWYVNRRAIAA
                     GLAGGVFFGLLLPTMQMPVAALWAILLRCNLPSALAGTFVSNPVTFVPIYLLAYRIGA
                     AILDVPFDESWTAILDQEASGFQAKLQLWWSAVYAAGVPLATGLLVVATASAALVYSA
                     VLLVWRTSTHRRYRQRPGRRGPGSDVDKM"
     gene            10121..11251
                     /locus_tag="VKZ48_02440"
     CDS             10121..11251
                     /locus_tag="VKZ48_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HLU76745.1"
                     /translation="MKEEKKPAVSRRKFLTGAAAAGAGAATLGFPAVVKAQGPVSMRW
                     QSTWPTKDIFHEYAVDFATKVNDMSGGDLKIEVLPAGAVVKAFDLIDAVSKGTLDGGH
                     GVVAYWYGKNSAMALWGSGPAWGMDPNMVLAWHKYGGGQQLLEEIYKSLNLNVVSFMY
                     GPMPTQPLGWFKKPVTKVDDMRGLKFRTVGLSIDIFSGMGLAVNAMPGGEIVPAMDRG
                     LLDAAEFNNASSDKVLGFPDVSKVCMLRSFHQCSEQFEVLFNKAKYDSLPQNMKALIA
                     NAVEAASADMSWKAIDRYSQDYIEMQKQGVKFYATPTSILQQQLKVWDEVVKKKEAEN
                     PMFKKVNASMREFAQRTTKWQSDTLVDFRIAQQHFFGGKKGS"
BASE COUNT         2003 a         3638 c         3828 g         2049 t
ORIGIN      
        1 tcgcggcaat aggcgtcgtc cttccatgct tcgatgcagc cgagcacctg tgcggcgaag
       61 gcttcgttga tctcccaggc atcgatgtcg tcgaggccga ggtcgtaggc tttcagcagc
      121 ggcgtggccg catgcacggg accgagcccc atgtgggccg gatcgagcgc ggcccagtgc
      181 gtatcgacaa tgcggccgat gggggtgagc ccgtactgcg caagtgcctc ctcggtggcg
      241 aggatcgtcc atacggcgcc gtcggtgatc tgcgaactgt tgcccgcggt cacggtgccg
      301 tattgcctgt cgaaaaaggg cttcagcttg gcgagattct cgaccgtgga atcggcgcgt
      361 acgccgtcgt cgcgggtgta ggcgttgccg cgatcgtcgt acatcggcac gatctcgtcg
      421 aaccagccgt tctcctgcgc ctgcgccacg cgttgatgac tgcgcgcggc gtattcgtcc
      481 tgttgcgtgc gcgtgatgcc gaagcggtag gcgaggttct ccgccgtctg gcccatgatc
      541 agaccgacgt tgggatcggt caggcctttc atgatgccga gcaccggcgc gagatactgc
      601 ggccggaatt tcgcgagcag cgccgctttc tggccgacgc tcttagcggc gtagaagttc
      661 gacagccaca tcaccatgcc ttcggacagc agcatgggcg cgcgcgacag cgcatcgacg
      721 ccgccggcca gcaccagatc ggaacgcccc gacatcatgt tggcgatggc cgagtcgagc
      781 gactgcatgc ccgaggcgca gttgcgcatc acggtccacg ccggcacatg ctgaccgcag
      841 cccatgcgca aggccgacac gcgtgcgatg ttgacctcgt ccggcgccgg caccgcgcaa
      901 ccgagtatga cctcatcgag ctgaaagggc tcgaagggct gacgcatcag cagtgcacgg
      961 ccggcgatgg tcgccagatc cgaggccgcg aacggaccgg gtttgttccg cgacttgaga
     1021 aagggcgtgc gtgcgccgtc gacgatgtag atcgctttgc tcatggtgtc ctttcctcag
     1081 gcaacggcgc gcgccgcgtc gctgcgtgcg tggtcggtga gcgacagatc gggcgggaag
     1141 tcgtccaccc ggatcacttc gttgcgcagt tgcgcggcac gcgcaatctg cgtctgttct
     1201 tccgcgctga tgatgcccgc gtccaccgcg gcctgagcgg tttcctcggg gctgcgcgcc
     1261 tcgatccgcc cggccttgac cgcgcggcgc accttggctt cgatcgcttc ggcggcgatg
     1321 gccgcatgca gtgcgcgttc cagcgtgccg atgatttcgc cgtcctcgcg gggaatgtac
     1381 atatcggcgg tcaggcggtc gcgcgtggag gaaggctcga tcaagagttc ggcgactttg
     1441 tgaccgagcc tgtccgaggc gacgacgtag ggccggccga gcgggaatac gaaccagcgc
     1501 agaatgtgcg cggcaacccg gttggggtaa ttcgacatca cgccttcgaa ggcgctttgc
     1561 gccttgaaca tcgcatccca gatcgcccag tcgagcagcg gcttgtcgac cgcgttgcgg
     1621 ccttcgtcct cgtagcgctt gagcgtcgcc gagcacaggt agagcaggga caggatgtcg
     1681 cccagccgcg cggaaagctt ttcgcgccgc ttgagttcgc cgcccatcac tgccatggaa
     1741 atgtccgcga cgaaggcgaa ggcggcggag aagcgcgtga gctgctggta gtagcgcttg
     1801 ttctccggcg ccacattcgc cggcacgcgc acgaagtgcg aaccggtcag acccatgaag
     1861 aaggcgcgcg cgccgttgca gagcgtgaag gagatgtgcc gccacagcgc ctggtcgaag
     1921 gcgcgcgcat cattctggcg cgcggccgtc agttccttca gtacataagg atggcagcgg
     1981 attgcgccct ggccgaagat gatcaggctg cgggtgagga tgttcgcgcc ctccacggtg
     2041 atggacacgg gaatctgctg ataagcacgg ccggtgaagt tgttcggccc cagacagatg
     2101 cccttgccgc cctggatgtc catggcgtcg ttgagcacgt cgcgcgcgcg ttcggtgacg
     2161 tggtatttga cgatgccgga aatgaccgaa ggcttctcgc ccaggtccag cgcgccggcg
     2221 gtcatgcgcc gcgccgcgtc catcatgtag aggttgccgc ccatgcgtgc gagcggctct
     2281 tcgacgccct cgaacttgcc gatgggtaga ttgaactgct ggcgcacgcg cgcataggcg
     2341 ccggtgccgc gcaccgccag cttggccatg ccggtgttgg aggatggcag cgatatcgag
     2401 cgccccgccg ccaggcactc catcagcatg cgccagccct ggccggccat tttcggtccg
     2461 ccgatgatcc agtcgagcgg catgaacacg tcttcgcccc agttcgggcc attcatgaac
     2521 acggcattga gcgggaagtg gcggcggccg atatggaccc cgggcgtgtc ggtcggcacc
     2581 agcgcacagg taatgcccag atccttctcg gcgccgagca gaccgtccgg atcgtagagg
     2641 cggaaggcca ggccgagcag cgtggcgatg gggccgagcg tgatgtagcg cttgtcccag
     2701 gtgacgcgca tgcccagcac ttcctcgccg ttccacatgc ccttgcacac gacgccccgg
     2761 tcgggaatgg cggccgcatc cgatcccgca tgcggactgg tgagtgcgaa gcagggaatt
     2821 tcctcgccgg cggccagacg cggcaggtag tagcgcttct gttcctcggt gccgtaatgc
     2881 agcagcaatt cggccgggcc gagcgagttg ggcaccatca ccgtcacagc cagcgcgctg
     2941 cagcgcgtcg acagcttggt gatcacctgc gaatgcatat aggcggagaa gcccaggccg
     3001 ccgtactcct tcggaatgat catgccgaag aagcgcttgt ccttgatgta ctgccacgct
     3061 tccggcggca gatcgttgat ggcgtaggtg tcccagtcgt tgcacagccg gcacaactct
     3121 tcgcattcgt tgtcgagaaa gctttgctcc tccggcgtga gcgtgggctt gggatagccg
     3181 agcagcctct cccagtccgg cttgccggag aacaactcgc cgtcccacca caccgtgccc
     3241 gcattgatgg cgtccgcctc ggtctgcgac atcggcggca tcaccttgcg gaagaccgcg
     3301 aggatgcgat tgctcaacag tacacgacgg atcggtggcg cgccgaagat caccacggcg
     3361 acggtgacga gtacgagcag ggtgtacagg gcgatcatga ggctcctcag gaggatgcgt
     3421 tgggcgacag gcctgtcggc gaccggtacg gcgcgggcgg tgtgagcgag ggttcctcgt
     3481 cgacgagcgg cgcgggcgcc tgcaggccgg cgagcagaaa gggcgtgagg cggcgcacca
     3541 gcatttcatc gtcgcgcccc gcgtccggct ggatggcggc gatgagtttc cacgcgtcgg
     3601 tgccggcgag ggtgtaggcc agtgcgccca tcatgaaatg cagccgccag gacagttcct
     3661 ggcgggacaa ctgcggcagc gcggcggcaa aggcctcctt gaagcgcgcg atggtcgggg
     3721 cgtagcgttc ggcgagcagt ttccgcagca ccggcgaagg atcgatgtag gcgcgaccga
     3781 gcaggcgcag gaaatcgcaa tccgcgtcgg cctcgcgtgc ccgtgccagc gcgggcttga
     3841 acagcgcggt cagcagttgt tcgcaggagg gcgtacggcc ttcggcctga taggcgtcga
     3901 gcagggccag ccgctcggca tgcagggagt cgaagcggcg cgccagcatc gcttcgaaca
     3961 gcgcgtcctt gccgccgaag tggtagttca ctgcgccaag gttcacgcct gccagcgaag
     4021 tgacgagccg cagcgaggtg ccgtcgaagc cgtgctccag gaacaggcgc tcggccgcct
     4081 ggatgatgcg ttcgcgggtc cgatcggcgc tgctcagggt tttgggggcc atgtacaaca
     4141 gagtcgcgac tccaaacgaa agtttcaaac gaccgtttgg acagcatacg acgccccgac
     4201 cgggtgtcaa gccgaaggat caacggccgg tgccgcggat acagcaaggg gcggagtgcg
     4261 agggtgcgat gccgccgcta gactgcccgt atggatgaac atcggagccg gagatgaaca
     4321 tttcgacgcc cagccggatc gcggcgcgga tcatcgctgc atgccgctcc atcgaggcgt
     4381 cggccacgac gcccgatctg gcggcgctgg ccgcgcaggc agaactcagt ccctcgcatt
     4441 tccatcggca gttcaaacgc ctcacgggtt tgacgccgcg cgagtacgcg cgcgctcacc
     4501 gcgaggcgcg cgtgcgtgca gcactcgctc agggcgcgcg tgtcaccgac gcgatgtacg
     4561 aagccggcta caactccaac ggccgcttct atgaggccgc cgatgcggcg ctcggcatga
     4621 agccgggtgc ctatcgcgca ggcggggcga acgcaaccat ccgctttgcc gtcggcgaat
     4681 gcagcctcgg ctcgatactc gtcgcgcaaa gcgggcgcgg catctgcgcc atcctcctgg
     4741 gcgaggaccc ggaagcgctg ctgcgcgacc tggagcggcg cttcccgcgc gcggaactga
     4801 tcggcggcga cgccgacttc gagcaactgg tggcgcgcgt ggtggggttt gtcgaagcgc
     4861 cgcggacggg actcgatctg ccgctggaca tccgcggcac ggcctttcag cagcgggtgt
     4921 ggcaggccct gcgtgccatc ccgcccggca cgacggtcag ctatgcggaa cttgcgcgcc
     4981 gtatcggcgc gcccaaggcg gtgcgtgcgg tgggcgcggc ctgcggcgcg aacgcgctgg
     5041 ccgtcgccat cccctgccac cgtgtcgtgc gcagcgacgg cgatctgtgc ggttatcgct
     5101 ggggcatcga gcgcaagcgc gccctgctcg atatcgaggc ggctgaaaag gactgaaaca
     5161 aatcttgaaa cacggagagc aaggagtgaa aaccgctgcg gtgcgtcttg ttgtggcgag
     5221 gtgatcgctt gcccgtgcgt atgccctcca aatcttgaac cacagagcat acacggagag
     5281 cctcgattgc aagctttctc cgtgttctcc gtggttctct gtgtttcaag cttttgagat
     5341 ttacaggccc ttgcttttgc gcgtttcgcc tcaacatagt gccgggtctg ctgccttcgg
     5401 cttccgcttt gaatccccat gcttccctgc cggcgaccga cgccgtcgca tcgccgcccg
     5461 cgccgccgcg caacgaatgg cgcgtcatcc gtctgctctg gccttatcta cgtgaatacc
     5521 gcggccgggt cgcgctggcg ctgatctttc tgaccgccgc caaactcgcc aacgtcggcg
     5581 tgccgctgct gttgaaggaa attgtcaatg cgctcgacag cgatacggcg gtgctggtga
     5641 tgccgctcgc gttgctcgtg gcgtacggcg cattgcgttt ctcgaccacg ctgttcgccg
     5701 agttgcgcga tctggtgttc atccgggtga cgcagcgcgc gatccgcagg gtcggcctgg
     5761 acgtattccg tcatctgcac agtctgagcc tgcgttttca cctggagcgc cagaccggcg
     5821 gcgtgtcgcg cgatatcgag cgcggcacca agggcatttc cacgctgctg tcctatctgc
     5881 tgttctcgat cattccggtg atcctggagt tcggactcgt cgccgccgtg ctgctggcgc
     5941 agttcgactg gcgtttcgcc gcaatcacgt tcatcgccgt cgggctttat atggtgttca
     6001 ccgtgctggt gacggagtgg cgcatcgcca tccgccgtcg cgcgaacgaa ctcgattcgc
     6061 aggccaatac gcgcgccgtc gacagtctca tcaactacga gacggtgaag tatttcaaca
     6121 acgaggagtt cgaggcgcgc cgttacgacc gcaatctgca gggctacgaa gcggcggcgg
     6181 tgaaaaacga agcctcgctc ggcctgctca acatcggcca gagtttcatc attgccatcg
     6241 cgctgaccct gctgatgatc ctcgccgccg acggcgtggt gcaagaaaaa ctgacactgg
     6301 gcgatctggt gctgatcaac ggcctgctga tccagctcta cattccgctc aacttcctcg
     6361 gcatggtgta tcgcgaaatc aagcaggcga cggtggacat ggatcgcatg ttcggcctgc
     6421 tcgacgagca tcgcgaagtg gaggaccgac ccgacgcgaa gccgctaccg gcgggggctt
     6481 tgtcggtgcg cttcgagcgc gtcaacttca gctatgagcc gaagcggcag atcctgtacg
     6541 aggtcgattt cgacataccg gcgggcagca aggtcgccgt agtgggacat tcgggctcgg
     6601 gcaagagcac gctcggccgt ctgctttatc gtttttacga cgtcacggcg gggcgcatca
     6661 ctgtaaacgg catcgacgtt cgggacctga aacagagcga tttgcgcggc gcgatcggca
     6721 tcgtcccgca ggacacggtg ctgttcaacg acaccatcgg ctacaacatc cgctacggcc
     6781 ggcccgaagc cagcgacgcg gaagtgatcg aggcggcgca ggcggcgcac atccacgact
     6841 tcatcgcggc gctgccggac ggctacgaga cccgcgtcgg ggaacgcggc ctgaagctgt
     6901 caggcgggga aaaacaacgg gttgcgatcg cgcgggcgct gttgaagcgg ccgcgcatcc
     6961 tgattttcga cgaggcgacc tcggcgctgg actccaaatc cgagcaggcg atccaggacg
     7021 aactgaaccg actggcgcgc ggacacacga cgctggtcat cgcgcaccgg ctttcgacca
     7081 tcatggatgc cgacgagatc atcgtgcttg atcgcggccg catcgtcgaa cggggtgggc
     7141 atcgtttctt gctaaaccag ggtggaccgt atgcgcagat gtgggcgctg cagcagcagg
     7201 agcgggaggc ggacgagctt gcgggtgcag cggcgggcgg cgcggaaacc tgacctggac
     7261 ggccggccag aagtgagcag aagcggaaat cctgctcatt tgctttttta agtacttcct
     7321 ccaagtcgtt attttcgttg atcgcgccgc gacacggtcg gtacactgtg tggggcttcc
     7381 gacagatcgg aagtagaggt caggacgggg tgagggaatg aagtcagtct gggcgcagac
     7441 ggcggtggcc gtcgtttttg cttttacgct gctgagcggg gcttatgccg cgagtgcgcc
     7501 gtcggaaccc cagatccgct cggcggcggt gctggtcatg gaccaggaaa ccggcgaagt
     7561 actgctcagc cagaacagcg gagacgtggt gcccatcgcc tcgctgacca agctgatgac
     7621 cgccctggtc acgcttgagg ccggcctgga cctcgaccag aaagtccgca tcaccaacga
     7681 cgacatcgac acgctcaagg gcacgggctc gcgtctgcgt gtgggcagcg tgttcacccg
     7741 cgacgagctg ctgaagcttg cgctgatgtc gtccgagaat cgcgctgctt ccgcgctcgg
     7801 ccgctcctat ccgggcggcg tctccgcctt cgtcaacgcc atgaacgcca aggcgagcga
     7861 aatcggcatg ctgcatacgc gcttcgtcga gccgaccggg ctgtcgagcg ccaacgtctc
     7921 gagcgcggag gatctggcca agctggtgcg cgccgcgcac ggcgtcccgc tcattcgcga
     7981 gtattccacc atgccgcggc acacggtcag gatcaaaggc cggccggcgc agttcgtcaa
     8041 caccaacgcg ctggtgcgcg ccggtcagtg ggacatcggc ctatccaaga ccggcttcat
     8101 cagcgaagcc gggcgctgcc tggtgatgca ggcgaaattc aatgcgcggt cggtcatcat
     8161 cgtgctgctc gactccttcg gcaaatacac ccgcgtggcc gacgccaagc gcatccgcgc
     8221 ctggctggac ccggatttca cgctcaagag ccagcagatc gtgaggtcga aggcggtcaa
     8281 ggtgaaagcc aagcgcaaca agtcctccaa tcgcgtgcgc acgcagatca aggcggccgc
     8341 ccgataggat ggcgggcacg gctccggcag gcgacgcgcg cagaccggac ccgctgtgcc
     8401 tccacgatct gcgcgggccg cagaccggtt ttctggacga cgtcctcgcc ggcctggcgc
     8461 agctgcaaaa gcgcattcca ccgaaatact tctacgacgc ggacggctcg cacctgttcg
     8521 aggccatctg cgcgctgccc gagtactacc ccacgcgtgc ggaaacggcg ctgatggacg
     8581 cgcatgcggg cgacatggcc acccggctgg gtgcggccat ccaattgatc gaattcggtt
     8641 ccggcgccag caccaagacg caacggctga tcgatgcggc gcggccttcg cgttatgtgc
     8701 ccatcgacat cagcgagtcg gcactgcgcg aagcctgctc gcgcttgcgc gcggcgtacc
     8761 cccatctgct cattcatgcc gtgtgcgcgg atttcaacgc gccgttgcag ttgcgtgcgc
     8821 ccatcctcgc ggacgaccgt ccgcgcgtgg tctatttccc cggctcgacc atcggcaact
     8881 tcacgcgggc cgaagcgcgg gcattcctgc agcgcgtcgc cgcgctgctc ggtccgggcg
     8941 ggcgtctgtt gatcggcgtg gatctgaaaa aggatcgcgc catcctggag gcggcctata
     9001 acgatgcgca gggcgtcacc gcgcgcttca atctcaatct gctcgaacgc atgaaccgcg
     9061 aactgggcgc ggatttccgg attgaacgct ttcgtcaccg cgcgttctat aacgcggagc
     9121 agagccgcat cgaaatgcat ctggacagcc ggatcgcgca gacggtccgc atcgccgggc
     9181 gcgaattttc tttccgcgcc ggcgaaagca ttcatactga gatctcctgc aagtacggct
     9241 tggccgaatt ccaggatctg gggcgcgcgg cggggtttga tccgctcgcc gtctggacgg
     9301 acgcggacgg gctcttcagc gtgcatctca tgcaggcggc gtaggcgctt ggcggcgccg
     9361 cgcgactgcc ggcgctttct gatcgctcat ggccaaacat tttttccggc gcgtgctgcc
     9421 cgatcccgaa tgggcagcgc ggcatcgttt cctcgcacga ctgggaccgc gcatcaccgc
     9481 cccgcaactg tggtacgtca accggcgcgc cattgcggcc gggcttgccg gcggcgtgtt
     9541 cttcggcctg ctgctgccca ccatgcagat gcccgtggcg gcgctgtggg ccattctttt
     9601 acgctgcaat ctgccctcgg cgctggccgg cactttcgtc agcaacccgg tcaccttcgt
     9661 accgatttat ctgctcgcct atcgcatcgg cgcggcgatc ctggacgtgc ctttcgacga
     9721 gagctggacg gcgatcctgg atcaggaagc gagcggcttt caggccaagc tgcaactgtg
     9781 gtggtcggcc gtctacgcgg cgggcgtgcc tttggcgacc gggctgctgg tcgtggcgac
     9841 cgccagcgcg gcgctggtct acagcgcggt gctgctggtc tggcggacga gcacccaccg
     9901 gcgctaccgc cagcggccgg ggcggcgggg gccgggatcc gatgtggaca aaatgtgaca
     9961 gatttcgcgt ctgtaacatc ctcccatcta cggtacatct tggtactaca atgcggtgac
    10021 aaaaatggat ggcggcataa atcgccgctg cataccagga cgccgtggcg agcggccgct
    10081 cccatgtgcg catttcgatc gcaagacaga ggagaagggt atgaaagagg agaagaagcc
    10141 ggccgtatcc cgtcgcaagt tcctcaccgg cgccgctgcg gcgggtgcag gtgccgcgac
    10201 gctcggattc cccgcggtgg tgaaggcgca aggacccgtt tccatgcgct ggcagagcac
    10261 ctggccgacg aaggacatct tccacgaata cgccgtggac ttcgccacca aggtgaacga
    10321 catgtccggc ggcgatctga agatcgaagt gctgccggcc ggagcggtgg tcaaggcttt
    10381 cgacctgatc gatgcggtca gcaaggggac cctggacggc ggccacggcg tggtcgcgta
    10441 ctggtacggc aagaactccg ccatggcgct gtggggctcc ggcccggcct ggggcatgga
    10501 cccgaacatg gtgcttgcgt ggcacaagta cggcggcggc cagcaactgc tggaagagat
    10561 ctacaagagc ctcaacctca acgtcgtctc cttcatgtac ggcccgatgc ccacccagcc
    10621 gctcggctgg ttcaagaagc cggtgaccaa ggtcgacgac atgcgcggcc tcaagttccg
    10681 caccgtgggc ctgtcgatcg acatcttcag cggcatgggg ctcgccgtga acgcgatgcc
    10741 cggcggcgaa atcgtgccgg ccatggaccg cggcctgctc gatgcggcgg agttcaacaa
    10801 cgccagttcg gacaaggtgc tgggcttccc cgacgtgtcg aaggtttgca tgctgcgcag
    10861 cttccatcag tgctccgagc agttcgaagt tctgttcaac aaggcgaagt acgactcgct
    10921 gccgcagaac atgaaggcgc tgatcgccaa cgcggtggaa gccgcctcgg ccgacatgtc
    10981 ctggaaagcg atcgatcgct attcccagga ctacatcgaa atgcagaagc agggcgtcaa
    11041 gttctacgcc acgccgacgt ctatcctgca gcagcagctc aaggtgtggg acgaggtggt
    11101 gaagaagaag gaggcggaga acccgatgtt caagaaggtc aacgcctcga tgcgcgagtt
    11161 tgcgcagcgc accaccaagt ggcagagcga cacattggtg gatttccgta tcgcgcaaca
    11221 acacttcttc ggcggcaaga aaggcagcta aggtgcttgc atcggtgccg gtcatgcgcg
    11281 gcgcagacgc cgcgcatgac cggccctcgt cgatctacgg cggaagatcg cggcttcccg
    11341 ccgcgcatcc gtctccggaa cgtgcgcata cgcgatttac ggtgatgcgg ggggcggcat
    11401 gtcgccgcgt cgcgcagcac gtaccgcctc tcacggccgc acgcgatcca gcttcgaacc
    11461 cgattcccgt ttccgaatgc aaaagcttct gtttgcggtc gaccggctga gcaccttc
//
LOCUS       DATGOU010000045         7768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_546780, whole genome shotgun sequence.
ACCESSION   DATGOU010000045 DATGOU010000000
VERSION     DATGOU010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7768
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546780"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..331)
                     /locus_tag="VKZ48_02445"
     CDS             complement(47..331)
                     /locus_tag="VKZ48_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HLU76746.1"
                     /translation="MSMDFMHGAITVLFVLVGLFFFRFWRDTLDRLFLLFAMSFWLQA
                     LTRIALTSVGGQEERAYLYLVRLVAFGLIIVAIAMKNLSRKSAKPPAADY"
     gene            complement(328..600)
                     /locus_tag="VKZ48_02450"
     CDS             complement(328..600)
                     /locus_tag="VKZ48_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HLU76747.1"
                     /translation="MSQLPAMVYVLCGLTALACVALLWRAYWRTGVRLLLWAGICFIG
                     LSIENIILFLDMEVVHGADLAFFRNAAALVGLLCLLYGLIWEVRAE"
     gene            complement(597..806)
                     /locus_tag="VKZ48_02455"
     CDS             complement(597..806)
                     /locus_tag="VKZ48_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76748.1"
                     /translation="MSAQGFLHSPSAGVYRYAPKESQMDAAVQDLAIVYEQLRVRVIE
                     YIYSRPSDSVRSFAHAFRIKGDPEE"
     gene            complement(1053..3215)
                     /locus_tag="VKZ48_02460"
     CDS             complement(1053..3215)
                     /locus_tag="VKZ48_02460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLU76749.1"
                     /translation="MKSTTSLMNSLSGKREAALPSGGMPSGSLAAPRVLVVDDNSSNL
                     AVFEAILTDPGFELVTAQSGVEAMRHLLSTDFAAILLDINMPTMDGLQTAQLIRERER
                     SRDVPIIFITAYQPDQAQLLAGYASGAVDYLVKPVSPEILKSKVRIFVELFRKSRQVE
                     WQARQLRAVNLQLQHEVQQREEAERDAAFEREERQSVTLASIADAVFTCDREGRVLSL
                     NPMAEHLTERRSDDVKGQLLSDVISTLGEGEEGLEALVTRCAAEDRLLRSSEPIQLLR
                     GASEARYLDYAAAPVHDRLGRVIGTVLVTHDVTTRHEMARERARALRLEQAARRIAED
                     TSRAREEFLAVISHELRTPLNAIVGWTHVLRTDGLNERYARQAVEAIHRSAMAQKKLI
                     EDLLDMSRIINGKIELVRQPVDLAAVVATAVDTLRPTADEKDVILQCSFGTPVDETLA
                     DRVRIEQVVWNVLSNAIKFTPDGGTVRVRLDRDDKQARIVVSDSGQGIPHEFLKHVFD
                     AFRQADSTTTRRQGGLGLGLAIARQLIVMHGGSIHAESAGVDQGTTVTITLPLVDCAG
                     ASTDAATQADFAADTSMQRGEALRDISVLVVDDDVNTLTLLSIALREQGAQVNAVSGP
                     DEGMRLLQLWRPQVLLSDISMPGEDGYSFIRRVRNLPAEEGGAVLGLALTAMASVEDR
                     AKALDAGFDGHVVKPLDLPALVDTLAHMLRGAGSAETP"
     gene            complement(3219..4046)
                     /locus_tag="VKZ48_02465"
     CDS             complement(3219..4046)
                     /locus_tag="VKZ48_02465"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HLU76750.1"
                     /translation="MIGETVPEALAALEIDLLMQAVGRAYALDTGRLPRPLVETTAAM
                     LVRRSGARSVSGLVERVLHEPAFGREALQALCACDVRLFHDWVFHRNVREQVVPWLRT
                     YPYSAVWLAECGAGGEVYSFAILLAECGLLDRVTIHATDRDPVLLAHARQGAVPLGVL
                     KEAQASYRRAGGTGSLSAHFVRQGDRGVLSEALQRRIVWSNYDLGTGHSFNEFHFIVC
                     RAALDELPAAGHRVLRLLTDSLSACGLLALNGDQRRILDPNRYRPWRPGTGIYQRTA"
     gene            complement(4043..>7768)
                     /locus_tag="VKZ48_02470"
     CDS             complement(4043..>7768)
                     /locus_tag="VKZ48_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017874992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLU76751.1"
                     /translation="NIADVTTAVARGDLSKKITVDVRGEILQLKDTINVMVDQLRSFA
                     SEVTRVAREVGTEGRLGGQATVPGVAGTWKDLTDSVNSMASNLTGQVRNIAEVTTAVA
                     RGDLSKKITVDVKGEILELKDTINVMVDQLNSFASEVTRVAREVGTEGKLGGQAKVSG
                     VAGTWKDLTDSVNVMATNLTDQVRGIAGVVTAVAQGDLKKKLTVEARGEIAALANTIN
                     EMTDTLAIFADQVTTVAREVGVEGKLGGQANVPGASGTWKNLTDNVNQLAANLTTQVR
                     AIADVATAVTRGDLTRSVTVRARGEVAILKDNINEMIRNLKETTQKNAEQDWLKTNLA
                     RFTRLLQGQRELETVVKLILSELAPLVGAQLGVFYMMEGAGESARLKLVAAYGYRARK
                     NLPAEFVPGEGVVGQCAVEQKRIIMSDVPPDYMRISSGLGSAVPRNVVVLPILFEQQV
                     KAVIELASFDAFSATHLAFFDQLMESIGVVLNNIEANTRTESLLKQSQTLARELQSGQ
                     EELQKTNDELAEKARLLSEQYIEVEHKNREVEQARLALEDKAAQLALSSKYKSEFLAN
                     MSHELRTPLNSLLILAQQLAENPTQNLTPKQVEYAKIIHGSGTDLLTLINDILDLSKI
                     ESGTVSLELRELSFDMLRDYVERTFRHMAEAKQLDFRIELEADLPATLYTDPVRLQQV
                     LKNLLSNAFKFTELGGVRLEVARAGEGWRLDNTALNATDTVIAFSVADTGVGIAPDKL
                     QLIFEAFQQADGTTSRRYGGTGLGLSISRELARLLGGEIVVESTPGQGSRFTLYVPLH
                     PAGEAAARTTSAIEGVRMPAKSLSASQEMRIPLPAVPDDRDCILSTEQFVLIVEDDPV
                     FAQILLDAARESGYKGLVTLQGNAAHALAREFQPCAVMLDLGLPDIEGWTVFDQLKHD
                     PDTRHIPVHVLSGQESMAERALRQGARAFLTKPVTHERLLEELASMRRYIDRPRKHLL
                     IVDDDEAQRTSLIELLDADDIHISTAATGEAALAVLARDQVDCVVLDLVLPDITGFEL
                     LERIAADAQWRTIPVVIYTGKELSRQEVTQLKRLAQSVIVKDARSPERLLDETALFLH
                     RHVARLSEAQRRMLASGFESDRALRGKKVLIVDDDVRNIFALTTVLERHHMTVSFAEN
                     GRDGIEALNGAPDTDIVLMDIMMPEMDGYDTMRAIRADARFATLPIITLTAKAMKGDR
                     EKCIEAGASDYIAKPVDTARLIALLRVWLAPRMGAAA"
BASE COUNT         1349 a         2607 c         2412 g         1400 t
ORIGIN      
        1 tgaaggacgt gttggagacg cccgaggcca aagccgccgc gaaggatcag taatcggccg
       61 ccggcggctt cgcgctcttg cgcgacaggt tcttcatcgc gatggcgacg atgatcaagc
      121 cgaaggcgac cagacgcacc agataaagat aggcccgctc ctcctgcccg ccgacgctgg
      181 tgagcgcaat gcgcgtcaat gcctgcagcc agaatgacat cgcaaacagc aggaacagtc
      241 ggtccagcgt atcgcgccag aagcggaaga aaaacaatcc gaccagtacg aagagcacgg
      301 tgatggcgcc gtgcatgaag tccatgctca ttccgctctc acctcccaga tcaggccata
      361 gagcaggcac agcaacccca ccagcgcagc ggcattgcgg aagaacgcca gatcggcccc
      421 gtgcacgact tccatgtcga ggaacaggat gatgttctcg atgctcagac cgatgaagca
      481 gatgcctgcc caaagcagca atcgcacacc cgtgcgccaa taagcgcgcc acaggagcgc
      541 gacgcacgcc agcgcggtga gaccgcacag cacatagacc atcgcaggca actggctcat
      601 tcttccgggt ctcctttgat gcggaatgcg tgggcgaagc tgcgcacgct gtccgaggga
      661 cgcgagtaga tgtactcgat gacgcgcacg cgcaactgct cgtagacgat tgccagatcc
      721 tgcacggccg catccatctg cgactccttg ggtgcatagc gataaacacc ggcggaaggc
      781 gagtgcagga aaccctgcgc actcatgcgt tcgagctgtt cttcggcggc gctgggcgcg
      841 acataaagca cctggctgat gtcgtacgcc cgccattccc gctcggcgtc gctactgacc
      901 agcagcagcg cttcgaggtc ggcaacggaa cggatgaatc gacggatgac gtccttgacg
      961 gattctggaa gccggcgaat aagggaacct cttcgtactg cgggttgacg actgggcgcc
     1021 ttccccgcgc ccgcgagccg tgaccgcccg acctagggcg tctcggcgga accggcgccg
     1081 cgcagcatgt gcgcgagcgt atcgacgagc gcaggcaaat cgagcggctt caccacgtga
     1141 ccgtcgaagc ccgcatcgag cgctttggcg cggtcctcca cgctcgccat ggctgtcaac
     1201 gccagcccca gcaccgcgcc gccctcctcg gcgggcagat tgcgcacacg acgaatgaag
     1261 ctgtagccgt cctcgcccgg catggagata tccgaaagca gcacctgcgg tcgccacaac
     1321 tgcagcaggc gcatgccttc gtccggcccg gagaccgcgt tcacctgggc gccctgttca
     1381 cgcaacgcga tggacagcag cgtgagggta ttcacatcgt cgtccaccac cagtacgctg
     1441 atgtcgcgca gcgcctcgcc gcgctgcatg ctcgtgtcag cggcaaagtc cgcttgcgtc
     1501 gccgcatcgg tcgacgcgcc ggcacagtcc accaacggca gcgtaatggt cacggtcgtc
     1561 ccctgatcga cgcctgcgct ttcggcatgg atggagccgc cgtgcatgac gatgagttgg
     1621 cgcgcgatgg cgagtcccaa tcccaatccg ccctgacggc gggtcgtcgt cgaatccgcc
     1681 tggcggaacg catcgaacac atgcttgaga aactcgtggg ggataccctg accggagtcg
     1741 ctgacgacga tgcgcgcctg tttgtcgtca cgatccagac gcacccggac cgtcccgccg
     1801 tcgggcgtga acttgatggc gttgctcaac acgttccaca ccacctgctc gatgcgcacc
     1861 cggtcggcca gcgtttcgtc cacgggcgtg ccgaagctgc attgcaggat cacatccttt
     1921 tcgtcggccg tgggacgcag cgtatcgacc gcagtggcca ccaccgcggc cagatcgacc
     1981 ggctggcgaa ccagttcgat cttgccgttg atgatgcgcg acatgtcgag caaatcctcg
     2041 atcagctttt tctgcgccat cgcgctgcga tgtatggcct ccaccgcctg gcgtgcatag
     2101 cgttcgttga ggccgtcggt gcgcagcaca tgcgtccagc ccacaatggc gttgagcggc
     2161 gtgcgcaatt catgcgagat cacggcgaga aactcctcgc gcgcccggct cgtgtcttcg
     2221 gcgatcctgc gcgccgcctg ctccaaccgc agggcgcgcg cgcgctcgcg cgccatttcg
     2281 tgtcgcgtcg tcacatcgtg cgtcaccagc accgtgccga tcacccggcc cagccggtcg
     2341 tgcaccggcg cggcggcata gtccagataa cgcgcttcgg aggcgccgcg cagtagttgt
     2401 atcggctcgc tgctgcgcaa cagccggtcc tcggcggcac agcgggtgac gagcgcttcg
     2461 agcccttcct cgccttcacc caaggtgctg atcacgtcgc tcagcaactg ccccttgacg
     2521 tcgtcgctgc ggcgttccgt cagatgctcg gccatgggat tgagcgacag gacgcggcct
     2581 tcgcgatcgc aggtgaacac cgcatcggca atgctcgcga gcgtcacgct ctgccgctcc
     2641 tcgcgttcga atgctgcatc gcgctcggct tcctcgcgct gctgaacctc atgctgcaat
     2701 tgcaggttga ccgcgcgcag ttgtcgagcc tgccattcca cctgccggct cttgcggaac
     2761 agctccacga aaatgcgcac cttcgatttg agaatctccg gcgacaccgg cttcaccagg
     2821 taatcgactg cgccgctggc ataaccggcg agcaactggg cctgatcggg ctggtaggca
     2881 gtgatgaaaa tgatcggcac gtcgcgcgaa cgctcgcgct cccggatgag ctgcgcggtc
     2941 tgcagcccgt ccatggtcgg catgttgatg tcgagcagaa tggcggcgaa gtcggtcgac
     3001 agcagatggc gcatcgcttc cacgcccgat tgcgcggtaa cgagttcgaa ccccgggtcg
     3061 gtgaggatcg cctcgaatac ggccaggttg gacgagttgt cgtcgacgac cagcacgcgc
     3121 ggcgcagcca ggctgccgct cggcatgccg ccggacggca gcgccgcttc gcgcttgccg
     3181 ctgagactgt tcatcaggct ggtcgtactc ttcatggttc acgcggtccg ttggtagatg
     3241 cccgtgccgg ggcgccacgg ccgataacga ttggggtcga ggatgcggcg ctgatcgcca
     3301 ttgagcgcaa gcagtccgca ggccgacagg ctgtccgtca gcaagcgcag cacccggtgg
     3361 ccggccgcgg gcagttcgtc gagcgccgcg cgacaaacga tgaaatggaa ttcgttgaaa
     3421 ctgtgccccg tgccgaggtc gtagttcgac cacacgatcc gccgctgcaa ggcctcgctc
     3481 aacactcccc gatcgccctg ccgcacgaaa tgcgcggaca ggctgcccgt gccgccggcg
     3541 cggcgatacg acgcttgcgc ttccttcaac acgccgagcg gcaccgcgcc ctggcgagcg
     3601 tgggcgagca gcaccgggtc gcgatcggtg gcatggatgg tgacgcgatc cagcagcccg
     3661 cattcggcga ggagaatggc aaagctgtaa acctcgccgc cggctccgca ttcggccagc
     3721 cacaccgcgc tgtacggata ggtacgtagc cacggcacga cctgctcgcg cacgttgcga
     3781 tggaacaccc aatcgtgaaa cagccgcaca tcgcaggcgc aaagggcctg aagcgcctcg
     3841 cgtccgaacg ccggctcgtg cagcacacgc tcgaccagcc ccgacacgct ccgcgcgccg
     3901 ctgcgacgca ccagcatcgc ggccgtcgtc tccaccagcg gacgcggcag gcggcccgta
     3961 tcgagcgcat aggcacgtcc gaccgcctgc atcaacaggt cgatttccag cgcggcgagc
     4021 gcctccggga cggtttctcc gatcatgccg ccgcccccat gcgcggcgcg agccagacgc
     4081 gcagcagcgc gatcaggcgc gccgtatcga cgggcttggc gatgtagtcg gaggcgccgg
     4141 cctcgatgca cttttcgcgg tcgcccttca tcgccttggc ggtcaacgtg atgatgggca
     4201 gcgtggcgaa gcgcgcatcg gcacggatgg cacgcatggt gtcgtacccg tccatctccg
     4261 gcatcatgat gtccatcagc acgatgtcgg tgtccggcgc gccgttgagc gcctcgatgc
     4321 catcgcggcc gttctcggcg aaggagacgg tcatgtggtg ccgctccagg acggtggtga
     4381 gcgcgaagat gttgcgcacg tcgtcgtcca cgatgagcac cttcttgccg cgcaaggcgc
     4441 ggtccgactc gaaaccgctg gccagcatcc gccgctgcgc ctccgacagc cgcgccacat
     4501 ggcgatggag aaacagcgcc gtttcgtcga gcagccgctc gggcgagcgg gcgtccttca
     4561 cgatgacgct ttgcgccagc cgcttgagtt gcgtcacttc ctgacggctc agttccttgc
     4621 cggtatagat gactacggga atggtgcgcc actgcgcgtc ggcggcgata cgttcgagca
     4681 gttcgaaccc ggtgatgtca ggcagcacca gatcgagcac cacgcaatcg acctgatccc
     4741 gcgccagcac cgccagcgcc gcctcgcccg tcgctgcggt gctgatgtgg atgtcgtccg
     4801 catcgagcag ttcgatgaga ctggtacgct gcgcctcgtc gtcatcgacg atgagcagat
     4861 gcttgcgcgg gcggtcgatg tagcggcgca tgcttgccaa ttcttcgagc aaccgttcgt
     4921 gcgtcacggg tttggttaaa aaggcgcgcg cgccctgacg cagcgcgcgt tcggccatgc
     4981 tctcctgccc ggacagcacg tgcaccggga tatgccgcgt gtccgggtcg tgcttgagct
     5041 ggtcgaacac cgtccagccc tcgatgtcgg gcagccccag gtcgagcatc accgcgcaag
     5101 gctggaattc gcgcgccagc gcatgtgccg cattgccctg cagcgtgacc agccctttgt
     5161 agccgctctc gcgcgccgca tcgagcagga tctgtgcgaa cacgggatcg tcctccacga
     5221 tgagcacgaa ctgttcggtg ctgaggatgc agtcgcgatc gtcgggcaca gccgggagcg
     5281 gaatccgcat ctcctgcgaa gccgacagcg atttggcggg cattcgcacc ccttcgatcg
     5341 cggatgtcgt ccttgccgcc gcctcgcccg ccggatgcag cggcacataa agcgtgaacc
     5401 ggctgccctg ccccggggta ctttcgacga cgatctcgcc gccgagcaga cgtgccagtt
     5461 cgcggctgat cgacaggccc aggccggtgc cgccgtagcg acggctggtc gtaccgtccg
     5521 cctgctggaa ggcttcgaaa atcagttgca gcttgtccgg cgcgatgccc acgccggtat
     5581 cggcaacact gaaagcgatc accgtatccg tggcgttgag cgccgtgttg tccagacgcc
     5641 agccttcgcc ggcgcgcgca acctccagac ggacaccgcc cagctcggtg aacttaaagg
     5701 cgttcgacaa cagattcttc agcacctgct gcagccgcac ggggtcggta tacagcgtgg
     5761 ccggcaaatc cgcttccagc tcgattcgga agtcgagctg cttggcctcc gccatgtggc
     5821 ggaaggtgcg ttcgacataa tcgcgcagca tgtcaaagga cagttcgcgc aactccagcg
     5881 ataccgtgcc ggattcgatc ttcgacagat cgagaatatc gttgatcagc gtgagcagat
     5941 cggtgcccga gccatggatg atcttggcgt actcgacctg cttgggcgtg agattctgcg
     6001 tcgggttctc ggcaagttgc tgcgcaagga tgagcagcga gttcagaggc gtgcgcagct
     6061 catgcgacat gttggcgagg aactcggact tgtatttgga tgacaacgca agctgtgcag
     6121 ccttgtcttc cagcgcgaga cgcgcctgct ccacctcgcg attcttgtgc tcgacctcga
     6181 tgtactgctc ggacaagagg cgcgccttct cggccagttc atcgttggtt ttctgcagct
     6241 cctcctggcc gctctgcaac tcgcgggcga gcgtctgcga ttgcttcagc agcgattcgg
     6301 tgcgcgtgtt ggcttcgatg ttgttcagca ccacgccgat cgattccatc aactgatcga
     6361 agaacgccag atgcgtggcg ctgaacgcat cgaaggaagc cagttcgatg accgccttga
     6421 cctgctgctc gaacaggatc ggcagcacca ccacgttgcg cggcacggcc gaccccagac
     6481 cggaggagat gcgcatgtag tccggcggca cgtcgctcat gatgatgcgc ttctgctcca
     6541 ccgcgcactg tcccaccacg ccttcgcccg gcacgaactc cgcgggcagg ttcttgcgcg
     6601 cgcgatagcc gtaggcggcc acgagcttga gtcgtgcgct ctcgcccgct ccttccatca
     6661 tgtagaacac gcccagttgc gcgccgacca gcggcgcgag ttccgacaga atcagcttca
     6721 ccaccgtttc cagttcgcgc tgtccctgca gcagacgggt gaagcgcgcg aggttggttt
     6781 tcagccagtc ctgctcggcg ttcttctgcg tggtctcctt gaggttgcgg atcatctcgt
     6841 tgatgttgtc cttgaggatc gccacctcgc cgcgcgcccg cacggtgacc gaacgcgtga
     6901 ggtcgccgcg cgtcaccgcc gtcgccacat ccgcgatcgc gcgcacttgt gtggtgaggt
     6961 tcgccgccag ttggttgacg ttgtcagtga ggttcttcca cgtgccggat gcgcccggca
     7021 cattggcctg cccgccgagc ttgccctcca cgcccacttc gcgcgccacg gtcgtgacct
     7081 gatcggcgaa gatcgccagc gtgtcggtca tttcgttgat ggtgttggcc agcgcggcga
     7141 tttcgccgcg cgcctccacg gtgagtttct tcttcagatc accctgcgcc accgcggtca
     7201 ccacgccggc gatgccgcgc acctggtcgg tgaggttcgt cgccatgacg ttcaccgaat
     7261 cggtgaggtc cttccacgtc cccgccacgc cgctcacttt cgcttggccg cccagcttgc
     7321 cctcggtgcc cacctcgcgc gccacgcgcg tcacttccga agcgaaggag ttgagctggt
     7381 ccaccatgac gttgatggtg tccttcaact ccaggatctc gcctttcaca tcgacggtaa
     7441 tcttcttcga caggtcgccg cgcgcaacgg ccgtcgtcac ttccgcgatg ttgcgcacct
     7501 gaccggtgag gttcgaggcc atggaattga ccgagtcggt caaatccttc catgtgccgg
     7561 ccacacccgg caccgttgcc tggccaccga ggcggccttc ggtaccgacc tcgcgcgcca
     7621 cacgcgtcac ttccgaagcg aaggaacgca actggtccac catcacgttg atggtgtcct
     7681 tgagttgcag aatctcgccg cgcacgtcca cggtgatctt cttcgacagg tcgccgcgtg
     7741 ccacggcagt ggtgacgtcg gcgatgtt
//
LOCUS       DATGOU010000046        12303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_4104086, whole genome shotgun sequence.
ACCESSION   DATGOU010000046 DATGOU010000000
VERSION     DATGOU010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12303
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4104086"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..654
                     /locus_tag="VKZ48_02475"
     CDS             52..654
                     /locus_tag="VKZ48_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLU76752.1"
                     /translation="MTMHELPVDIRPLDAAELAAAAKLCACAMRRNPVHLRVFGKTPK
                     RLDRRLRRFFAAALPYIQRKGALWGAYVDSRLIGVMGAIPPGACALDAREMLQILPHL
                     AMSPAALLRLHRWLQVWRHNDLTEPHWHLGPLAVEPSHQSRGVGSRLLHHCFTHAGND
                     DALYLETDTPGNVRFYESFGFRVLKTLPVLGTPTWCMRRD"
     gene            complement(655..2094)
                     /locus_tag="VKZ48_02480"
     CDS             complement(655..2094)
                     /locus_tag="VKZ48_02480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HLU76753.1"
                     /translation="MSDDVAASPRLIYQLPALRRDAQGRRQVSYRAVFALATPLLLNT
                     AIQAILSLTDTWFIGRLSTSATAAMGATYYLVLVFVLLFGGIGMAVQTQVAQAYGGRR
                     RVRAAKAVWIGAWAAAFTIPPFVLLALSGHGLLGPFGLSPEIEDLAVEYWLPRMLGGP
                     LAVLYWVFTGFFNGIGRTRLTLFIAIAMALLNAGLNELFMFRFGWGMAGAGWATTASV
                     AIAIVIAMALFLTSFVHEEYKSRLTWRPEWRVLGRSLALGLPMGLSSAVDLIGLSVFQ
                     LMQVRLGAIDGAASQIAMMLTSLAYMPAIGFGIAGTTLVGQSIGAGDRDWAYRVGNAT
                     IKLSTAYMGCVGILLALAAPWVLPWFVADGDPYAQEVMRLAQVLLWLAAGYQLFDGMH
                     LGSVFCLRGAGDVRFPAVLVVGLSWFGFVPLAYVFTFAPGQGWFDLPLQMGWGATGGW
                     IAAVVYIFALGVVINWRWRSGAWRRITVR"
     gene            2201..2842
                     /locus_tag="VKZ48_02485"
     CDS             2201..2842
                     /locus_tag="VKZ48_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76754.1"
                     /translation="MSDATFASAVQALRAAFPVVATVTGPHRFREYVALYRSPYPYDG
                     SPQYGDEFAEFLDVMQVEDMPYLPELAQLEWLVHCARQADDVPTIDAATLSAIPTEKF
                     GETRLALHPSAGLQQAGYPVARLWEIHQPGFAGQPTVEAVIVRNYILVNRPGTHVEVS
                     TIDEGGYAFLNAIARGATLGEACALARAAKADFDLAEALQDAAARRLLCTAAG"
     gene            complement(2912..3223)
                     /locus_tag="VKZ48_02490"
     CDS             complement(2912..3223)
                     /locus_tag="VKZ48_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLU76755.1"
                     /translation="MPLNRKDRQFLQKGAAQAAGLLRTVGNEHRLLVLCLLIEHGELS
                     VGEVLAHVDLSQSALSQHLAKMRDEGLVTYRREAQTLYYRVDNPAVEKLIATLKSIYC
                     P"
     gene            3313..4626
                     /locus_tag="VKZ48_02495"
     CDS             3313..4626
                     /locus_tag="VKZ48_02495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HLU76756.1"
                     /translation="MPHQTIDRRRFLQLSAGGAAALLLAAPEVRAEKIKTRARIVIAG
                     AGAAGLTVAARLAARLDGARITLIDARKEHYYQPGFTLIAAGVKPANYVVSTTAEYVP
                     HGVELVRERVAEFDPEGNKVVTADGKSHAYDFLVVATGLKLDYAAIEGMDEKLIGSNG
                     LGSIYHSPAGALATWRLLSEFADRGGTGVFLRPATEMKCAGAPLKYTFITEDHLTRRG
                     NRGKAEIIYNAHNNALFSVPIVSEKVRMLFGERGIAVNYDRVLRAIDPGRRIATFAAP
                     GGDVELGYDFINVIPPMRAPDAVRNSPLPWQSGEFAADGWMEVDRGNLRHVRFANVYA
                     VGDVAGVPKGKTAASVKWQAPVVVDHLVADISGVPTRSLYDGYTSCPLITRLGRAMLV
                     EFDYRNNLRPSFPGVIAPLEELWVSWVMKTMALKPTYISMLRGRA"
     gene            4636..4983
                     /locus_tag="VKZ48_02500"
     CDS             4636..4983
                     /locus_tag="VKZ48_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5368 domain-containing protein"
                     /protein_id="HLU76757.1"
                     /translation="MKELDPLVLLAVFQELLGPLLWLLILLAVGGTLAFVVVLIRERK
                     VHARRLVLSQLAGIAGGALALVLMAKVSSSGFTDAAGPIDWLLIALVFGLGLVGTTIL
                     VYTAAGWRALRRR"
     gene            5010..5384
                     /locus_tag="VKZ48_02505"
     CDS             5010..5384
                     /locus_tag="VKZ48_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HLU76758.1"
                     /translation="MSAPRYQSLTREISGNLNAMRSGLPQVMQGFNALAKAAMADGAI
                     DAKTKELIALAIGVSTRCDGCIGFHSQALVRLGATPDEVRETLGIAVYMGGGPSLMYA
                     ANAIAAFEEFSAAETARMASIG"
     gene            complement(5395..7077)
                     /locus_tag="VKZ48_02510"
     CDS             complement(5395..7077)
                     /locus_tag="VKZ48_02510"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HLU76759.1"
                     /translation="MERIWLRSYPAGVPADIDYHQYRSLGELFDECVSNYAERTAYHH
                     MGRDMSFAELERASRAFGAWLQAHGQAKGARVALMMPNVLQYPIGIFGTLRAGCVVVN
                     TNPLYTPRELEHQLTDSGAETIVILENFAHTLQQIIGRTQVKHVIIASMGDMLGLKGS
                     LVNLVVRRVKKMVPAYELPGARRWNDVLAEGERLTLAPVDIGHDDLAFLQYTGGTTGV
                     AKGAMLTHRNIIANVLQAEAWLKPFLSKTQSEVVINALPLYHIFSLTVNCLLFLKAGG
                     CSVLITNPRDIPGLVKEMGKHRFSVFSGVNTLFNALLHNPDFAKLDFSSLRVSLGGGM
                     AVQKQVADKWKEVTGCTLVEAYGLTETSPGATVNPLDIEAWNGTIGLPISSTEVEIRD
                     DDGKPVPIGQPGEICIRGPQVMAGYWNRPEETAKVMHADGYLASGDIGIMDERGFVRI
                     VDRKKDMIVVSGFKVFPNEVEAECAQHPGVLECAAVGVPDEHSGEVVKVYVVRKDASL
                     TERVLQEHLHDRLTGYKRPHYVEFRDQLPKSNVGKILRRELRDEALKKTETA"
     gene            7355..8290
                     /locus_tag="VKZ48_02515"
     CDS             7355..8290
                     /locus_tag="VKZ48_02515"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HLU76760.1"
                     /translation="MQDMDFDLQGHRGARGLMPENTLPAFARALEIGVSTLELDCGIT
                     ADDVVVVHHDLRLNPDITRCDGRWLSRRGPAIRSLRYEELTAYDVGAIRPGSAYARRF
                     PAQQPLEGVRIPRLADLFALVAASQAGHVHFNIETKIDPTQPALTVDPAAFARALVAE
                     IHKAGLNARAIIQSFDWRTLAVVQQEAPDIRTAYLSSQALDFDTVRPVRDRASPWTNG
                     LHFYDFRSLPKMVGAAGGHIWSPRHEDLDTGLIQEAHELGLQVIPWTVNEPGDMARLI
                     DWGVDGLITDYPDRLREVMAAKRLALPPAVRAASA"
     gene            8408..8620
                     /locus_tag="VKZ48_02520"
     CDS             8408..8620
                     /locus_tag="VKZ48_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2970 domain-containing protein"
                     /protein_id="HLU76761.1"
                     /translation="MPERRTEQPSTLRRTLRAMKAVAWGFFGVRRRSGHESDIKLGPV
                     QIIIAGLIAAAIFIGTLIFVVSLVVQ"
     gene            8748..9434
                     /locus_tag="VKZ48_02525"
     CDS             8748..9434
                     /locus_tag="VKZ48_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HLU76762.1"
                     /translation="MTALLASLCAAASAAPEDTMAQRMRACTACHDNQGRQGSDAYYP
                     RIAGKPYEYLYNQLRNFRDGRRQYPLMAGLLHEMSDEYMLEIAHYFAALDVPYAKRGG
                     GTLAPEQAARARKLVESGDESRRLPGCAACHGDALMGVQPAVPGLLGLPTDYIRAQLG
                     AWHSGTRKAASPDCMAEIAQSLTSEEISIVSAWLSSQPVPADAKPAPRATREAPMECG
                     SVLDAQKGER"
     gene            9431..10705
                     /locus_tag="VKZ48_02530"
     CDS             9431..10705
                     /locus_tag="VKZ48_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLU76763.1"
                     /translation="MRVIKRALGVLGVLICVAVILLVWRNFRDEDAHPSAVADFAQEE
                     FIARGAYLARVGHCAGCHTARGGAPYAGGYGVQTPFGTVYASNLTPDPETGIGDWTSD
                     DFWRALHNGRSKDGRFLYPAFPYPNYTQVTREDADAIYAYLRSLEPVHAPNQAHSLRF
                     PYRTQFALGVWRALYFAPGVYEPDDAQSDEWNRGVYLVKGFGHCSACHAGRNTLGASH
                     EQLDGGPLPVQGWYAPSLQSAREAGVAHWEPERIVALLRDGVTAGASVIGPMAEVVHG
                     STQYYSEADLNAMAAYLKTLPMDDPPPANFDPVRRSHYARGERIYADHCADCHGEQGE
                     GVRGMYPALADNRAVTLDTPTNVVSAVLRGGFPPTTAGNPRPFGMPPYATLLSDEDIA
                     AVVSYIRNAWGNQASSVTAWEVQRHGRGVRGR"
     gene            10785..>12303
                     /locus_tag="VKZ48_02535"
     CDS             10785..>12303
                     /locus_tag="VKZ48_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HLU76764.1"
                     /translation="MSQPSRAAGAPTPEASGTGHVTNFIRNLVDADLASGRYDGRRWP
                     GGPGPARLKAEAPADPAKIRTRFPPEPNGYLHIGHAKSICLNFGLARDYGGVCHMRFD
                     DTNPEKEEQEYVDAILDAVKWLGFDWGEHLYFASDYFDWMYEFAELLIRNGHAYVDSL
                     SADEMRAYRGTLTEPGKNSPYRDRPVEENLDLFRRMRAGEFKEGEHVLRAKIDMASPN
                     INMRDPAIYRIRHAAHHRTGDKWCIYPMYTYAHPVEDALENITHSLCTLEFEDQRPFY
                     EWVLDRIADAGGLLRPLPTQTEFARLNLTYVVLSKRKLIQLVQENHVEGWDDPRMATI
                     VGFRRRGFTPESIRLFCERIGVSKADSWIDMTVLEDCLREDLNERALRRIAVLDPLKL
                     VIENYPDGEGEACYAPNHPQKPELGKRAVPFSRELWIEREDFKENPPKGYFRLAPGAR
                     VRLRYGYIVECTGFEKDADGNVTLVRCNYLPDSKSGTPGADQYKVKGNIHWLSAAH"
BASE COUNT         2184 a         4213 c         3862 g         2044 t
ORIGIN      
        1 gaagttgccc gctgactgcc tgaagacgca tacacgggga tttgcgatgc catgacgatg
       61 cacgaactgc ctgtagacat acgtccgctc gacgccgccg agcttgccgc ggcggccaag
      121 ctctgtgcct gcgccatgcg ccggaacccc gtccacttgc gtgtcttcgg caagacgccg
      181 aagcgtctcg atcgccggct gcgacgcttt ttcgccgcgg cgctccctta tattcagcgc
      241 aagggcgcgt tgtggggcgc ctatgtcgac agccgtctga tcggcgtaat gggtgcgata
      301 ccgccgggcg catgtgcgct cgacgcgcgc gagatgctgc agatcctgcc gcatctggcg
      361 atgagccccg ccgcactgct gcgtctgcat cgctggctgc aggtatggcg ccacaacgat
      421 ttgaccgagc cgcactggca tctcggtcca ctggcggtgg agccgagtca tcagagccgc
      481 ggcgtaggca gccgtctgct gcatcactgt ttcacgcacg cggggaacga tgacgcgctg
      541 tatctggaaa cggatacgcc aggcaacgtc cgcttctacg aaagcttcgg ttttcgcgta
      601 ctgaaaacgc tgccggtgct cggcacgccg acctggtgca tgcgtcgcga ttgatcagcg
      661 caccgtgatg cgccgccacg cgccggagcg ccagcgccag ttgatgacca cgccgagcgc
      721 gaaaatgtag accaccgcgg caatccagcc gccggtcgca ccccagccca tctgcagcgg
      781 cagatcgaac cagccctggc ccggcgcgaa cgtgaacaca taggccagcg gcacgaagcc
      841 gaaccacgac aaacccacca ccagcaccgc ggggaagcgc acgtcgcccg cgccgcgcaa
      901 gcagaacacg ctgcccagat gcatgccgtc gaagagctga taaccggcgg ccagccacag
      961 cagcacctgc gcaagccgca tcacctcctg cgcgtaggga tcgccgtcgg cgacgaacca
     1021 gggcagcacc cagggcgcgg ccagcgcgag cagaatgccg acgcacccca tgtacgcggt
     1081 cgacagcttg atggtggcgt tgcccacgcg gtaggcccaa tcgcgatcgc ccgcgccgat
     1141 cgactgcccg accagcgtgg tgccggcgat gccgaagccg atggccggca tgtaggcgag
     1201 cgaagtcagc atcatcgcga tctgcgaagc cgcgccgtcg attgcgccga gccgcacctg
     1261 catcaactgg aacaccgaca gcccgatgag atccactgcg ctggacagtc ccatcggcag
     1321 gcccagcgcg agcgagcgcc cgagtacacg ccattcgggc cgccaggtga ggcggctctt
     1381 gtattcctca tggacgaagg aagtcaggaa cagcgccatc gcgatgacaa tggcgatcgc
     1441 caccgatgcg gtggtggccc agcccgcgcc ggccatgccc cagccgaagc ggaacatgaa
     1501 caactcgttc aggccggcgt tgagcaaggc catcgcgatc gcgatgaaca gcgtgaggcg
     1561 cgtacggccg atgccgttga agaagccggt gaacacccaa tagagcaccg ccagcggccc
     1621 gccgagcatg cgcggcagcc aatattcgac ggcgagatcc tcgatctccg gcgacaggcc
     1681 gaaaggtccg agcagaccgt gacccgaaag ggccagcagc acgaaaggcg gaatggtgaa
     1741 tgccgccgcc cacgcgccga tccataccgc cttggcggcg cgcacgcggc gccggccgcc
     1801 gtaagcctgc gcgacctgcg tctgcacggc catgccgatg ccgccgaaca agagcacgaa
     1861 caccagcacc agatagtagg tcgcgcccat cgcggcggtg gcactggtgg acaggcggcc
     1921 gatgaaccag gtgtcggtca gcgacaggat cgcctggatc gcggtattga gcagcagcgg
     1981 cgtggccagc gcgaagacgg cgcgatagct gacctgccgg cgaccctgtg catcgcgccg
     2041 gagcgcgggc aattgataga tcagacgggg tgacgcggcg acgtcgtcgg acatgcggga
     2101 gaaaacgggc gggggaaata agcaagcccg gcatgttaac gtgcacgcac cgcgcacacc
     2161 accgcatgca ccgggcgcgc ggcctgcaca ggagattttg atgtcggatg caaccttcgc
     2221 cagcgcggtt caagccttgc gcgccgcttt tcccgtcgta gcgaccgtga ccggcccgca
     2281 tcgcttcagg gagtatgtgg cactttatcg cagcccctat ccttacgacg gctcgccgca
     2341 atacggcgac gagttcgcgg agtttctgga tgtcatgcaa gtggaggaca tgccctatct
     2401 gcccgagctc gcgcaactgg aatggctggt gcattgcgcg cggcaggccg acgacgtgcc
     2461 cacgatcgat gcggccacat tgagcgccat accgacggag aaattcggcg agacgcgtct
     2521 tgcactgcat ccgtctgccg ggttgcagca ggcgggctat ccggtcgcgc gcctatggga
     2581 aattcatcaa ccgggcttcg cgggccaacc gacggtggag gcggtgatcg tacgcaacta
     2641 catcctggtc aatcgaccgg gcacgcatgt ggaagtgtcg accatcgacg aaggcggcta
     2701 cgcctttctg aacgcgatcg cgcgcggcgc cacactcggc gaagcctgcg ccctggcacg
     2761 cgccgccaaa gccgacttcg acctggccga agccttacag gacgccgctg cgcgccgact
     2821 gctctgcacg gctgccggct aagcaccgtc ggcctgctcg cggcacggag gatgcgcttc
     2881 ctcagcgtgg ggccggtcgt cggccaccgc ttcagggaca gtagatgctc ttgagcgtgg
     2941 cgatcagctt ttcgacagcg ggattatcca cgcggtaata aagcgtctgc gcttcgcgac
     3001 gataggtcac caggccttcg tcgcgcattt tcgccagatg ctgcgacagc gccgactggc
     3061 tcagatccac atgcgcgagc acctcgccca cgctcaattc gccgtgttcg atcaacaggc
     3121 acagcaccag cagccggtgt tcgttgccca ccgtgcgcag caggcccgcg gcctgcgccg
     3181 cgcccttttg caaaaactgc cggtcttttc tgttaagcgg cattcttttt acctggatca
     3241 attagcgctt tctaatttag attagtctaa tccacattgc gctccggcgc acccggtaaa
     3301 agggagaacg ccatgccgca tcagaccatc gatcgccgac gcttcctgca actgagtgca
     3361 ggcggcgccg ccgcgctgtt gctcgccgcg cccgaagtgc gcgccgagaa gatcaagacg
     3421 cgcgcgcgca tcgtcattgc cggtgcgggc gcagccggcc tcaccgtcgc cgctcgcctc
     3481 gccgcgcggc tcgacggcgc ccggatcacg ctcatcgacg cgcgcaagga acactattac
     3541 cagccgggct tcacgctcat tgcagcaggc gtcaagccgg caaattacgt cgtctccacc
     3601 acggcggagt acgtgccgca cggcgtcgaa ctggtgcgcg agcgcgtggc cgagttcgat
     3661 cccgaaggca acaaggtggt gacggcggac ggcaaatccc acgcctacga tttcctggtg
     3721 gtcgccaccg gcctcaagct cgactacgcc gccatcgagg gcatggacga gaagctgatc
     3781 ggcagcaacg gcctgggcag catctatcac agtcccgccg gcgcactggc cacctggcgg
     3841 ctgctgtccg aattcgccga ccgcggcggc accggcgtct tcctgcggcc cgccacggaa
     3901 atgaagtgtg ccggcgcgcc gctcaaatac accttcatca ccgaggatca tctgacgcga
     3961 cgcggcaatc gcggcaaggc cgagatcatc tacaacgccc acaacaacgc gttgttcagc
     4021 gtgccgatcg tgtcggagaa agtgcgcatg ctgttcgggg aacgcggcat tgccgtcaac
     4081 tacgaccgcg tgctgcgcgc catcgatccg ggccgccgca tcgccacctt cgccgcgccg
     4141 ggcggcgatg tggaactggg ttacgacttc atcaacgtca ttccgcccat gcgcgcgccg
     4201 gatgcggtgc gcaacagtcc cctgccctgg cagagcggcg aattcgccgc cgacggctgg
     4261 atggaagtcg atcgcggcaa tctgcgccat gtgcgcttcg ccaatgtcta tgcggtgggc
     4321 gacgtggccg gcgtgcccaa aggcaagacc gccgcgagcg tgaaatggca ggcgccggtg
     4381 gtcgtcgatc atctggtcgc cgacatcagc ggcgtaccga cccggtcgct ctacgacggc
     4441 tacacctcct gcccgctgat cacgcgcctg gggcgggcga tgctggtgga gttcgactac
     4501 cgcaacaacc tgcgaccgtc cttccccggc gtgatcgcgc cgctggaaga actgtgggtg
     4561 agctgggtca tgaagaccat ggcgctgaaa cccacttaca tcagcatgct gcgcggacgc
     4621 gcctgaggag ccgccatgaa agaactcgat ccgctggtcc tgctcgccgt cttccaggaa
     4681 ctgctcggcc ccctgctgtg gctgctgatt ctgctcgccg tcggcggcac gctggccttc
     4741 gtcgtcgttc tgatccgcga gcgtaaggta cacgcccgac gcctggtgct gtcgcagctt
     4801 gccggtatcg ccggcggcgc gctcgcactg gtactgatgg cgaaagtgtc ctcctccggc
     4861 ttcaccgatg cggccggccc gatcgactgg ctgctgattg cgctggtgtt cggcctcggg
     4921 ctcgtcggca cgaccatcct cgtctatacc gccgcagggt ggcgtgccct gcggcgtcgc
     4981 tgatttccga ttctcaaggc ttgaacgaca tgtccgcacc ccgctaccaa tccctcaccc
     5041 gcgagatcag cggcaacctc aacgcaatgc gcagcggttt gccgcaagtc atgcagggct
     5101 tcaatgcgct cgccaaggcg gcgatggccg acggcgccat cgatgccaag accaaggagt
     5161 tgatcgcgct ggcgatcggc gtttccacgc gctgcgacgg ctgcatcggc tttcacagcc
     5221 aggcgctggt gcggctgggc gcgacgccgg acgaggtgcg cgagacgctg ggcatcgccg
     5281 tgtacatggg cggcggaccg tcgctgatgt acgcggccaa tgcgattgcg gccttcgagg
     5341 aattctccgc cgccgaaacg gcgcggatgg cttcgatcgg ctgaggaccg cgactcaggc
     5401 ggtctcggtc ttcttcagcg cttcgtcgcg cagttcgcga cgcaggatct tgccgacatt
     5461 gctcttcggc aactgatcgc ggaactcgac gtaatgcggc cgcttgtagc cggtcaggcg
     5521 gtcgtgcagg tgctcctgca gcacccgctc ggtaaggctc gcatccttgc gcacgacgta
     5581 gaccttcacc acttcgcccg aatgctcgtc gggcacgccg accgcggcgc attccagcac
     5641 gccgggatgc tgcgcgcatt cggcttccac ttcgttgggg aagaccttga agccggacac
     5701 tacgatcatg tccttcttgc gatcgacgat gcgcacgaaa ccgcgctcgt ccatgatgcc
     5761 gatgtcgccc gacgcgagat aaccgtccgc atgcatgacc ttggcggttt cctccggccg
     5821 gttccagtag ccggccatca cctgcggccc ccggatacag atctcgcccg gctgaccgat
     5881 cggcaccggc ttgccgtcgt cgtcgcggat ttccacttcg gtagaggaaa tcggcaggcc
     5941 gatggtgccg ttccacgctt cgatgtcgag cgggttcacc gtcgcgccgg gtgaagtctc
     6001 ggtgaggccg taggcttcca ccagcgtgca gcccgtcact tccttccact tgtccgcgac
     6061 ctgcttctgc accgccatgc cgccgccgag cgacacgcgc aggctggaga aatcgagttt
     6121 ggcgaaatcg gggttgtgca gcagcgcatt gaacagcgtg ttcacgccgc tgaagacgct
     6181 gaagcgatgc ttgcccatct ccttcaccag tcccggaatg tcgcgcggat tggtgatcag
     6241 cacgctgcag ccgcccgcct tgaggaacag caggcaattc accgtcagcg agaagatgtg
     6301 atagagcggc agcgcgttga tgaccacttc gctctgcgtc ttcgacagga agggtttgag
     6361 ccacgcctcg gcctgcagca cgttggcgat gatgttgcga tgcgtgagca tcgcaccctt
     6421 cgccacgccg gtcgtgccgc ccgtgtattg caggaaggcc agatcgtcgt gaccgatgtc
     6481 caccggcgcg agcgtcagcc gttcgccttc ggccagcaca tcgttccagc gccgcgcgcc
     6541 cggcagctca taggccggca ccattttctt cacacggcgc acgaccagat tcacgaggct
     6601 gcccttgagg cccagcatgt cgcccatgct ggcgatgatc acgtgcttca cctgcgtgcg
     6661 gcctatgatc tgctgcagcg tgtgggcgaa gttctccagg atgacgatcg tttccgcacc
     6721 ggaatcggtg agctggtgct ccagttcacg cggggtatag agcggattgg tgttgaccac
     6781 cacgcagccg gcgcgcagcg tcccgaagat gccgatcgga tactgaagca cgttgggcat
     6841 catcagcgca acccgcgcgc ccttcgcctg accgtgcgcc tgcagccagg cgccgaaggc
     6901 gcgcgaagcg cgctccagtt cggcgaagct catgtcccgg cccatgtggt gataggccgt
     6961 gcgctcggca tagttgctga cgcattcgtc gaacaactcg cccagggaac ggtactggtg
     7021 atagtcgatg tccgccggca ccccggcggg ataactccgc agccaaatac gttccacgtc
     7081 gcctcctctt tgcgtaccgt tgttataggc ccggcaccgt gagcacaagt atgaaggctt
     7141 gccgtccgct gtcaaacacg gccctgcgcc agttcacacc aaccgttcgc cctgagcgct
     7201 cgccgcaggc gagcagtcga agggtgaacg acggtctttc cgttcgctac gagtacccgt
     7261 ccatggttcg actcggccct ggggccggct cgccacgaac gggacctggc gtgccccatg
     7321 ccatcacgta cactgcgcgc ttcgacatgc ccccatgcaa gacatggact tcgatctgca
     7381 aggccaccgc ggcgcgcgtg gcctgatgcc ggaaaacacg ctgcccgcct tcgcgcgggc
     7441 actggaaatc ggcgtgagca cgctggagct ggattgcggc attacggcgg acgacgtcgt
     7501 ggtggtgcat cacgacctgc gcctcaaccc cgacatcacg cgctgcgacg gccgctggct
     7561 gagccggcgc ggtccggcga tccgcagcct gcgctacgag gaactgaccg cctacgatgt
     7621 gggcgccatc cgccccggca gcgcctacgc gcgccgcttc ccggcacaac agccgttgga
     7681 gggcgtgcgc attccgcgtc tggcggactt gtttgcgctg gtcgcggcgt cgcaggccgg
     7741 gcacgtgcac ttcaacatcg aaaccaagat cgatccgaca cagccggcgc tcaccgtcga
     7801 tccggccgcc ttcgcccgcg cgctcgtcgc ggagatacat aaagctgggt tgaacgcgcg
     7861 cgccatcatc cagtccttcg actggcgcac gctcgcggtg gtgcagcagg aagcgcccga
     7921 catccgcacc gcgtatctca gcagtcaggc gctggacttc gacaccgtcc ggcccgtgcg
     7981 cgaccgcgct tcaccgtgga ccaacggcct gcacttctac gatttccgtt cgctgccgaa
     8041 aatggtcggt gccgccggcg gccacatctg gtcgccgcgg cacgaggatc tggacaccgg
     8101 cctgattcag gaagcgcacg aactgggctt gcaggtcatt ccctggacgg tgaacgaacc
     8161 gggcgacatg gcgcgactga tcgactgggg cgtggacggc ctcatcaccg actatcccga
     8221 tcgcctgcgc gaagtcatgg cggcgaagcg gctcgcgctg ccgcccgccg tgcgcgccgc
     8281 atcggcttga attgcacgat gcgagccgca tctgtataga caaactcaat ctctttcggc
     8341 cgcatgatcg acgcgcgatg cgcgcatcgc ttatgcttgg gccatcgcaa cggagcgcct
     8401 ccacaacatg cctgaacgcc gtacggaaca gccgtccacc cttcgtcgga ctctgcgcgc
     8461 catgaaggcg gtcgcctggg gttttttcgg cgtgcgacga cgcagcggcc acgaaagcga
     8521 catcaagctc gggccggtgc agatcatcat cgcgggcctc atcgccgcgg cgattttcat
     8581 cggtacgctg atcttcgtgg tctcgctcgt cgttcaataa acgcgcaccg cattcggtgt
     8641 tcgcgcgctt ggcgttagaa tgaaaaaggc cggcgccagc cagccgctgt cccgtccttc
     8701 ttcattccat gcaacgcaac cgccttcttt cccatacacg cgtcttcttg accgcgctgc
     8761 tcgcgagcct ttgcgccgcg gcgagcgccg cacccgaaga caccatggcg cagcgcatgc
     8821 gcgcctgcac cgcctgtcac gacaatcaag gccgccaggg gtccgacgcg tactacccgc
     8881 gcatcgccgg caagccctac gaatatctct acaaccagtt gcgcaatttc cgcgacggcc
     8941 gacgccagta cccgctcatg gccgggttgc tgcacgagat gtcggacgaa tacatgctgg
     9001 agatcgcgca ctacttcgcc gcgctcgacg tgccctacgc caagcgcggc ggcgggacgc
     9061 tggcgcccga gcaagcggcg cgcgcacgca agctggtgga aagcggcgac gaatcgcgac
     9121 gcctgcccgg ttgcgccgcc tgtcacggcg acgcgctgat gggggtgcag ccggcggtgc
     9181 ccggcctgct cggcctgccg accgattaca tccgcgcaca actgggcgcg tggcatagcg
     9241 gcacgcgcaa ggcggcatca cccgattgca tggcggagat cgcgcaaagc ctgacctccg
     9301 aggaaatttc catcgtctcc gcgtggctgt cgtcgcaacc cgttccggcc gatgccaagc
     9361 ccgcgccgcg cgcaacacgc gaagcgccga tggaatgcgg cagcgtgctc gacgcgcaga
     9421 agggcgagcg atgagggtga tcaagcgcgc gctcggcgtt ctcggcgtgt tgatatgcgt
     9481 cgccgtcatc ctgctggtgt ggcgcaactt ccgcgacgag gacgcgcatc cctccgcggt
     9541 tgcggatttc gcgcaggagg aattcatcgc gcgcggcgcc tatctcgccc gcgtgggaca
     9601 ttgcgccggc tgtcacaccg cgcggggcgg cgcgccctac gccggcggct acggcgtgca
     9661 aacgcccttc ggcacggtgt acgcctcgaa cctcacgccc gatcccgaaa ccggcatcgg
     9721 cgactggacg agcgacgatt tctggcgcgc cctgcacaac ggccgctcga aggacggccg
     9781 cttcctgtat ccggccttcc cttatccgaa ctacacgcaa gtgacgcgcg aggacgccga
     9841 cgcgatctac gcctacttgc gcagcctgga gccggtgcat gcgccgaatc aggcgcacag
     9901 cctgcgcttt ccgtaccgca cgcagttcgc gctgggcgtg tggcgcgcgc tttatttcgc
     9961 gcccggcgtg tacgaaccgg acgacgcgca atcggacgaa tggaatcgcg gcgtctatct
    10021 ggtgaaaggg ttcggccatt gcagcgcctg ccacgccggc cgcaacaccc tgggcgcttc
    10081 gcacgaacaa ctcgacggcg gccccctgcc cgtacagggc tggtacgcgc cctcactgca
    10141 gtccgcgcgc gaagccggcg tcgcgcactg ggaacccgag cgcatcgtcg cactgctgcg
    10201 cgacggcgtg acggccggcg cctcggtcat cggcccgatg gccgaagtgg tgcacggcag
    10261 cacgcagtac tacagcgaag ccgacttgaa cgcgatggcg gcctatctca agacgctgcc
    10321 gatggacgat ccgccgccgg ccaacttcga cccggtccgg cgttcgcatt acgcacgcgg
    10381 cgaacgcatt tacgccgacc attgcgccga ctgccacggc gaacagggcg aaggcgtgcg
    10441 cggcatgtat ccggcgctcg cggacaatcg cgccgtcacg ctggacacgc ccaccaatgt
    10501 ggtgagcgcc gtgctgcgag gcggcttccc gccgaccacc gcggggaatc cgcgcccctt
    10561 cggcatgccg ccttatgcga cgctgctcag cgacgaggac atcgccgcgg tcgtttccta
    10621 tatccgcaac gcctggggca atcaggcctc gtccgtcacc gcctgggaag tccagcgtca
    10681 cggacgcggc gtcagggggc gctgacgcag cacacgcgcg cggcttcggc cggagtcgct
    10741 aaggtagaat ccacacgatt gattcttccg cctgcgatcc gacaatgtcc cagccgtccc
    10801 gtgcagcggg cgccccgacg ccagaagcgt ccggcaccgg ccacgtcacc aatttcatcc
    10861 gcaatctcgt cgacgccgat ctggcgagcg gccgctacga cggccgccgc tggcccggcg
    10921 gtcccggtcc cgcccggctc aaagccgagg cgcccgccga tccggcgaag attcgcacgc
    10981 gcttcccgcc ggagccgaac ggctatctgc acatcggtca cgccaaatcg atctgcctca
    11041 atttcggcct cgcgcgcgat tacggcggcg tgtgccacat gcgcttcgac gacaccaacc
    11101 cggagaagga agagcaggaa tacgtcgatg ccatcctcga cgcggtgaag tggctcggct
    11161 tcgactgggg cgagcatctc tacttcgcct ccgactactt cgactggatg tacgagttcg
    11221 cggaactgct gatccggaac ggccacgcct acgtggacag cctgtccgcc gacgaaatgc
    11281 gcgcctaccg cggcacgctg accgagccgg gcaagaacag tccctatcgc gatcgccccg
    11341 tcgaggagaa cctcgatctc ttccgccgca tgcgtgcggg cgagttcaag gaaggcgagc
    11401 acgtgctgcg cgccaagatc gacatggcct cgcccaacat caacatgcgc gacccggcga
    11461 tctaccgcat ccgccacgcc gcgcatcatc gtacgggcga caagtggtgc atctatccga
    11521 tgtacaccta tgcgcatccc gtcgaggacg cgctggagaa catcacgcac tcgctgtgca
    11581 ctttggagtt cgaggaccag cgccccttct acgaatgggt actggatcgc atcgccgatg
    11641 ccggcggtct gttgcggccg ctgcccaccc aaacggaatt cgcgcgactc aatctcacct
    11701 acgtcgtgct gtccaagcgc aagctcatcc agctcgtgca ggagaatcac gtcgagggtt
    11761 gggacgatcc gcgcatggcg accatcgtcg gcttccgtcg ccgcggtttc acgcccgagt
    11821 ccatccgact gttctgcgag cgcatcggcg tgtcgaaggc tgattcctgg atcgacatga
    11881 cggtgctgga agactgcctg cgcgaagatc tcaacgaacg cgcgctgcgc cgtatcgccg
    11941 tgctcgatcc gctcaagctg gtgatcgaaa actatccgga cggcgaaggc gaggcgtgct
    12001 acgcgcccaa ccatccgcag aagccggagc tcggcaagcg tgccgtgccc ttctcgcgcg
    12061 aactgtggat cgagcgcgaa gacttcaagg agaatccgcc caagggttat ttccgtctcg
    12121 cgcccggcgc acgcgtgcgg ctgcgttacg gctatatcgt cgaatgcacg ggcttcgaga
    12181 aggacgcgga cggcaacgtc accctggtgc gctgcaacta tctgcccgat tccaagtccg
    12241 gcacgccggg cgcggaccaa tacaaggtga agggcaacat ccactggctg tccgccgccc
    12301 acg
//
LOCUS       DATGOU010000047         7037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_1889594, whole genome shotgun sequence.
ACCESSION   DATGOU010000047 DATGOU010000000
VERSION     DATGOU010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7037
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889594"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1170)
                     /locus_tag="VKZ48_02540"
     CDS             complement(73..1170)
                     /locus_tag="VKZ48_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLU76765.1"
                     /translation="MLELRLAQQSPIPLDAQIRCETGELLALVGPSGSGKSTILKTIA
                     GLMRGPVGRIVVNGDVWLDSASGAHVPAHRRRVGFVFQSYALFPHMTVLENVLAACDA
                     LREEDRLAQAYKWIEAVRMKGLEDARPARLSGGQQQRVALARALVREPQLLLLDEPFS
                     AVDQLTRERLYEELAELRSRLSIPVVFVTHSLPEAQMLADRMVVLHHGRTLQSGTPDE
                     IFLRPRTAEIARLVAHKNLGEAVVLGHDDRYTHVDWQGMQLAAAACPDLPPGTRVNWL
                     IPPLDVRLVDMRFDDYTGRENIHCAEVESMVPLGGQVTVRVRMPNGQRLTLTATAHIV
                     RKRAVEVGRVVDVSLLAESIHLMPREPEAAG"
     gene            complement(1173..1823)
                     /gene="modB"
                     /locus_tag="VKZ48_02545"
     CDS             complement(1173..1823)
                     /gene="modB"
                     /locus_tag="VKZ48_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HLU76766.1"
                     /translation="MDWEALRLSLLLALATSVVLLPLGTMTARALAWSRFRGKWLLEA
                     ALTLPLVLPPTVLGYYLLVAMGAASPLGAFYERVFGHPLAFSFEGLIVASVIFNIPFA
                     LIPMQRAFEAIPLEVHQAALTCGISPLRSFFKVELPLAWPGVLSGMVMTAAHTVGEFG
                     VVLMVGGAIPGETKTIAISIYDRVQAFDMQAAGMMSAMLLVISLFALGFTTRMGRR"
     gene            complement(1824..2609)
                     /gene="modA"
                     /locus_tag="VKZ48_02550"
     CDS             complement(1824..2609)
                     /gene="modA"
                     /locus_tag="VKZ48_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HLU76767.1"
                     /translation="MMCLTRRLFLTLLLLFPSVYTPAAAAQDVPTVAAAASIQYALEE
                     IAAAYERDTGAKVRLVFGSSGNLSRQIVQGAPFHLFMSADEHFVYDLRKRGAAVDEGV
                     VYAVGRVALFVPKGSAIEADAQLADLARAIDDGRLRKFAMANPEHAPYGVAAQEVLEH
                     KGLWTELRGRTVMGESISQAAQYVASGSAQAGLIALSLATQPNFRRAGSYVLLPEAWH
                     TPLRQRMVVLKRGGESARNFYDYLQQPAAQAIFKKHGFETGEP"
     gene            complement(2719..3510)
                     /locus_tag="VKZ48_02555"
     CDS             complement(2719..3510)
                     /locus_tag="VKZ48_02555"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLU76768.1"
                     /translation="MNQLSRADMRRVAALWRDLADAPAAECDAALRHCMARLQTLVGA
                     CNATWVGVNRQRPAADDPLGGWRIREVDHLHHRERRMVFARELNERLRAGQHDPYVGA
                     IVRRAGHTRSHLREELVPAAIWRESWIVREALAEERIGDRLAGAHSVSRHSESHLVLD
                     RAHGDRPFGARERDLLQFFLLGSRSFHRELLLSRGLIDASAPFSPRERDVLRLLLTDA
                     GEKEIARVLNIGHRTVHQHAASIYAKLGVRGRQGLMAQWLRRRGS"
     gene            3615..5573
                     /locus_tag="VKZ48_02560"
     CDS             3615..5573
                     /locus_tag="VKZ48_02560"
                     /inference="COORDINATES: protein motif:HMM:NF015831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HLU76769.1"
                     /translation="MHRTTRLVSGFALALSLGWLSSPPAWATDNCDGNSLSPSGSIGT
                     DATACGLANTAPGAYSVAMGTNNRATGDASVAVGLGNNSIGRRSAALGSGNTADLGFS
                     TAVGTSNSALGESSSALGFSNIAYGTGSSAIGVYTHARSHYSTALGAYGQVQSLLADG
                     SLYSIAIGAGDSHATSAAVGTNGGAGAQAAIAIGHRVSVADGAHDSVAIGTGATVDAN
                     VTGAVALGAGAVATESNTVSVGASGAEKRIVNVAAGTAATDAVNKGQMDTAVATALDA
                     AQSFATNAANTAETNAKAYTDTVASTTLNDAKTFATNAANAAQANAINAANSYTDTQV
                     ATVAASASNAQTTANTALANAATAQGAASAAQVDASAALANIQTAVNQLLQSGVCSMT
                     GGTVSCGGAVQLGNGSTASGANAVAVGNGASASYDGAVAIGDGATITAPTSGGPTAGA
                     VAIGRGARANADPSVAIGNFADATGADAVAIGDSSTATGNQAVALGYQAVATHANSVA
                     LGAGSQTSAADTVSVGRVGANRRITNVATPVDADDAATKGYVDAMIGGGGANVLNEAK
                     AYTDRQVSAARDYAARGIAATAAIVSVTPSAVGKTAIGIGTGHYDGHSAVGMSVAHAP
                     HARVLLSAGVAAANGGRAVFRAGVAFEF"
     gene            complement(5686..5895)
                     /locus_tag="VKZ48_02565"
     CDS             complement(5686..5895)
                     /locus_tag="VKZ48_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HLU76770.1"
                     /translation="MRISARNVLRGKVIEVKKGQTTAHVRLQLPNGEVVTSAITNESV
                     DDLGLKVGDEAAAIIKSSDVMIGKD"
     gene            complement(6088..6336)
                     /locus_tag="VKZ48_02570"
     CDS             complement(6088..6336)
                     /locus_tag="VKZ48_02570"
                     /inference="COORDINATES: protein motif:HMM:NF022832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HLU76771.1"
                     /translation="MDIKILVKMANQIETFFRSQDEDGNAIAEGVRNHIERSWDPRMR
                     QQIKGYVEAGGEGLGEFAIEAVKRLQPLSPQWGGSKVA"
     gene            complement(6407..>7037)
                     /gene="fdhD"
                     /locus_tag="VKZ48_02575"
     CDS             complement(6407..>7037)
                     /gene="fdhD"
                     /locus_tag="VKZ48_02575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HLU76772.1"
                     /translation="IVACREDVRRVRVRNLAEGMELDIEIDPARHRTLDRRRRALTGR
                     VGCGLCGQESLLQAVRKPSPVPNKLKLPHTAVPTAVQELPQWQALNRVTGALHAAAWV
                     NLDGEVLAVREDVGRHNAFDKLIGCLLNDRTDFSRGFALITSRASHEMVQKAASVGIE
                     ALVAVSAPTTLAVRLAETANVTLIAFARGERYTVYSGHERMIGAALTAV"
BASE COUNT         1305 a         2414 c         2196 g         1122 t
ORIGIN      
        1 atcgatctcc tctgtgacat ccggggcggc attgcccgac acgtcccaga ggcaagaaac
       61 ggacgcgccc gctcagcccg ccgcttcggg ctcccgcggc atgagatgga tgctttcggc
      121 gagcagcgat acgtcgacga cgcgtccgac ttccaccgcg cgtttgcgca cgatatgcgc
      181 cgtggcggtg agcgtcagcc gctggccgtt gggcatgcgc acgcgcaccg tcacctgtcc
      241 gcccaggggc accatggact ccacttccgc gcaatggatg ttttcgcgcc cggtgtaatc
      301 gtcgaagcgc atgtcgacca gccgcacgtc gagcggcgga atcagccagt tcacgcgcgt
      361 accgggcgga agatcgggac acgccgcggc ggcaagctgc atgccctgcc aatccacgtg
      421 cgtataacgg tcgtcgtggc cgagcacgac cgcttcgccc agattcttgt gcgcgaccag
      481 ccgggcgatt tccgcagtgc gcggacgcag gaaaatctcg tccggcgtgc cgctttgcag
      541 cgtgcggccg tgatgcagaa cgaccatgcg atcggccagc atctgcgctt cgggcagcga
      601 atgggtgacg aacaccaccg gaatggacaa gcggctgcgc aattcggcca gctcctcgta
      661 cagccgctcg cgcgtgagct gatcgacggc cgagaaaggc tcgtcgagca gcagcagttg
      721 cggctcgcgc accagcgcgc gcgccagcgc cacgcgctgc tgctgaccgc cggagagtcg
      781 tgcggggcgc gcgtcctcca gccccttcat gcgcacggcc tcgatccatt tgtaagcctg
      841 cgcgagccgg tcttcctcgc gcagcgcatc gcaggcggcc agcacgttct ccaacaccgt
      901 catgtgggga aacagcgcat agctctggaa gacgaagccc acgcgccgcc ggtgtgccgg
      961 cacgtgggcg ccgctcgcgc tgtcgagcca gacgtcgccg ttgacgacga tgcgccccac
     1021 cgggccgcgc atcagaccgg cgatggtctt gagaatggtg gacttgcccg agcccgaggg
     1081 accgaccagc gccagcagct cgcccgtctc gcagcggatc tgcgcatcga gcggaatggg
     1141 cgactgctgc gcgagacgga gttccagcat ggtcagcggc gtcccatacg cgtggtgaag
     1201 ccgagcgcga acagcgagat caccagcagc atggcggaca tcatgcccgc cgcctgcata
     1261 tcgaaagcct gcacacgatc gtagatcgag atggcgatgg tcttcgtctc gccgggaatg
     1321 gcgccgccga ccatcagcac cacgccgaat tcgccgacgg tgtgcgctgc ggtcatcacc
     1381 atgccggaca ggacgcccgg ccacgccagc ggcaactcga ccttgaagaa agacctgagc
     1441 ggcgaaatgc cgcaggtcag cgcggcctga tgcacttcca gcggaatcgc ctcgaatgcg
     1501 cgctgcatgg gaatcagcgc aaacgggata ttgaaaatca ccgaggcgac gatcaggcct
     1561 tcgaaggaga aagccagcgg atggccgaac acacgctcat agaaagcgcc gagcggcgag
     1621 gccgcgccca tcgccacgag caggtaatag ccgagcaccg tgggcggcag cacgagcggc
     1681 agcgtcaggg cggcctccag cagccatttg ccgcggaagc ggctccatgc gagcgcacgc
     1741 gcggtcatcg ttcccaacgg caacagcacc acgctcgtcg cgagcgcgag cagcagcgac
     1801 aggcgcagcg cttcccaatc caactatggc tctcccgttt cgaaaccgtg ctttttgaaa
     1861 atcgcctgcg cggcaggctg ctgcaggtag tcgtagaagt tgcgcgcgga ctcgccgccg
     1921 cgtttgagca cgaccatgcg ctgccgcagc ggtgtatgcc acgcttcggg cagcaatacg
     1981 tagctgcccg cccgtctgaa attcggctgc gtcgccaggg acagggcaat gagtcccgcc
     2041 tgcgccgagc cgctggccac atattgcgcc gcctgcgaaa tgctctcgcc catcacggtc
     2101 cggccgcgca gttcggtcca caagcccttg tgctcgagca cttcctgcgc tgcaacgccg
     2161 tagggcgcgt gctccggatt ggccatcgcg aactttctca agcgtccgtc gtcgatcgca
     2221 cgtgcaagat cggcgagttg cgcgtccgcc tcgatcgccg accccttggg cacgaacagc
     2281 gccacgcgcc cgacggcata caccacgccc tcatccaccg cagcaccgcg cttgcgtagg
     2341 tcgtaaacga aatgctcgtc ggcagacatg aagagatgga acggcgcgcc ctgcacgatc
     2401 tgacggctga gattgcccga ggaaccgaac accagacgca ccttggcgcc cgtgtcccgc
     2461 tcgtaggcgg cggcaatctc ctccagcgca tactggatcg atgcggcggc agcgacggtc
     2521 ggcacgtctt gcgcggcggc ggctggcgtg taaaccgacg ggaacagcag cagcagtgtc
     2581 aggaacagcc ggcgcgtaag gcacatcatg aaagttgaag ccgcgccagg ggcggcgagt
     2641 acgaagcgcc gtcactataa cgaagcgcgg cgcatgccgc tacgcgccgg gcgaactacg
     2701 tcgagcggct aatggcgctc aggaaccgcg tcggcgcagc cactgcgcca tcaacccctg
     2761 tctgccgcgc acgccgagct tggcatagat gcttgcggcg tgctgatgca ccgtacggtg
     2821 gccgatattc agcacgcgcg cgatctcctt ctcaccggcg tcggtcagca gcagacgcag
     2881 cacatcgcgc tcgcgcggac tgaagggggc ggatgcgtcg atcagtccgc gggacaacag
     2941 cagttcccga tgaaaactcc tgctgccgag cagaaagaac tgcagcagat cccgctcgcg
     3001 tgcaccgaac gggcgatcgc cgtgcgcccg atcgagcacc agatgcgatt cgctgtgccg
     3061 gctgacgctg tgtgcgccgg caaggcggtc gccgatccgt tcctctgcca gcgcctcacg
     3121 cacgatccag ctctcgcgcc atatggcggc cggcacgagt tcctcgcgca agtggctgcg
     3181 ggtatgcccc gctcgacgca cgatggcacc gacataagga tcgtgctgtc ccgcacgcag
     3241 acgttcattc agttcgcgcg caaagaccat gcgacgttcg cggtgatgca gatgatccac
     3301 ctcgcgaatg cgccacccgc ccagcggatc atcggcggcg ggacgctgcc ggttgacacc
     3361 gacccaggtc gcattgcagg cgccgaccag cgtctgcaat cgcgccatgc aatggcgcag
     3421 tgccgcgtcg cattcggcgg ccggcgcatc ggccaagtcg cgccacaacg ccgccacccg
     3481 gcgcatatcc gccctgctga gctgattcat gtccggctcc tccccaggct tccggttctc
     3541 ctccgctaat gagcggatgg taagggcgct gcgcccccgc gacgattgca tggatcacat
     3601 ttaggacacc ggccatgcac cgcacgacac gacttgtctc aggattcgcc ctcgccctta
     3661 gcctcggctg gctgagctcg ccccccgcct gggccaccga caactgcgat ggaaactcgc
     3721 tcagcccctc cggcagtatc ggtactgacg cgaccgcatg cgggcttgct aatacggcac
     3781 caggtgccta cagcgtggcg atgggaacga acaatagagc cacgggcgac gcgagtgtgg
     3841 ccgttggcct aggcaataac agcatcggta ggcggagtgc cgcactcggg tctggcaaca
     3901 ccgcagacct cggattcagc acagcagtcg gcactagcaa ctcggcactg ggcgaatcca
     3961 gcagcgcctt gggcttcagt aatattgctt acgggacggg gagtagcgcg atcggcgtct
     4021 atacccacgc tcgcagccac tacagcaccg cgctcggcgc ttacgggcag gtgcaaagtc
     4081 ttcttgcgga cggatcgctg tattccatcg ccattggcgc cggcgacagt catgccacga
     4141 gcgccgccgt cggcacgaac ggtggggccg gcgcccaggc ggctattgcc atcgggcatc
     4201 gggtctcggt ggccgatggc gcccacgatt ccgttgccat cggcacagga gcaaccgttg
     4261 acgccaatgt cacgggcgcc gttgcactcg gcgcgggcgc tgtagcgacc gaaagcaaca
     4321 ccgtatcggt gggcgcttcc ggcgctgaaa agcgcattgt gaacgtcgct gccggcacgg
     4381 ctgccaccga cgccgtaaac aagggtcaga tggatacggc agtcgccacg gcgctcgacg
     4441 ccgcgcagag cttcgcgacc aatgcggcga acactgccga gacaaacgcc aaggcctata
     4501 ccgataccgt ggcatcaacg acactcaacg acgccaagac cttcgccacc aatgctgcga
     4561 acgccgcgca ggccaacgcg atcaacgccg cgaacagcta cacggacacg caggtggcca
     4621 cggtggcggc cagcgccagc aatgcccaga ccacggcgaa cacggcgctt gccaatgcgg
     4681 caacggcaca gggcgccgcg agcgcggcac aggtcgacgc cagcgccgca ctggcgaaca
     4741 tccagactgc cgtcaatcaa ctgctgcagt cgggcgtgtg ctcgatgacc ggcggcaccg
     4801 tctcctgcgg cggcgccgtg caactgggta acggcagcac ggcaagcggc gccaatgcgg
     4861 tcgccgtcgg caacggcgcc tcagcctcct atgacggggc cgtcgcgatc ggcgacggcg
     4921 ccaccatcac cgcaccgacc agcggcggcc cgaccgcagg cgcggtggcc atcggccgcg
     4981 gcgcacgcgc caatgccgac ccgtccgttg cgatcggcaa cttcgccgat gccaccggcg
     5041 cggacgccgt cgcgatcggc gacagcagca ccgccacggg caatcaggcg gtggcgcttg
     5101 gctatcaggc agtcgccaca cacgcgaaca gcgttgcgct gggcgcaggc tcgcaaacct
     5161 ccgccgccga tacggtcagc gtgggccgcg tgggcgcaaa tcgtcgcatt acgaacgtcg
     5221 ccacgccggt cgacgcggac gatgccgcaa ccaaaggcta tgtcgatgcg atgatcggcg
     5281 gcggcggcgc gaatgtgttg aacgaagcga aggcctacac ggatcgccag gtgtccgctg
     5341 cgcgcgatta cgctgcacgt ggtatcgccg ccaccgcagc gatcgtcagc gtgacgcctt
     5401 cggccgtagg caagaccgcc atcggcatcg gcaccgggca ttacgacggt cacagcgccg
     5461 tcggcatgtc cgtcgcccat gcgccgcatg cgcgtgtgct gctgtccgca ggcgtcgccg
     5521 ccgcgaatgg cggcagggcc gtgttccgcg cgggcgtggc cttcgaattc tgaggagcgc
     5581 gtccagtcgc ttgagacgcg cggcaaagca cagggcatgg actgccctgc caatgcctgg
     5641 atagcccgcc acataccacc ggcaggcaag tgtgctacgg caaaatcaat ccttgccgat
     5701 cattacgtcc gaggacttga tgatggcggc ggcttcgtca cccactttca agccaagatc
     5761 atcgaccgac tcgttggtga tcgccgaggt gaccacttca ccgttcggca gttgcaaccg
     5821 tacatgtgcg gtcgtctgcc ccttcttcac ttcgatgact ttgccacgca ggacgttgcg
     5881 tgcactgatt ctcatgatgg cgctcctgga ggttgacgcc cgcagcctat atgcccgtta
     5941 gagcactatc aatatgtccg atgtgcgcat atttggcggc ctgtcgcccg cgacagacgc
     6001 ccaaaaaaaa tgcgacgcct caagaggcgt cgcagtgaaa gtgcggccgg tcccatccgg
     6061 ccgcgaggct ccgttgaaca gccttcttta tgcgaccttg ctgccacccc actgagggct
     6121 cagcggctgg agtcgcttga ccgcttcgat ggcgaactcg cccagtcctt ccccgcccgc
     6181 ttcgacatac cccttgatct gctgacgcat gcgtggatcc caggagcgtt cgatgtgatt
     6241 gcgtacgccc tcggcaatcg catttccgtc ctcatcctgc gagcgaaaga aagtctcgat
     6301 ttgattcgcc atcttgacca gtatcttgat gtccatttaa tccaaccccg ttttcatgat
     6361 tcgcccgtcc tggtgacggg ccacttgatc ttgtacggcg atccgctcag accgccgtca
     6421 gcgcagcgcc tatcatccgc tcgtgcccgc tgtacaccgt gtagcgctcg ccgcgcgcga
     6481 aagcgatcaa tgtgacgttc gccgtttccg ccaaccgcac cgccaacgtg gtcggcgccg
     6541 acacggcaac caatgcttcg atgccgaccg atgccgcctt ctgcaccatt tcgtgacttg
     6601 cacggctggt gatcaacgcg aaacctcgtg agaaatccgt acggtcgttg agcaggcaac
     6661 caatcagctt gtcgaaggcg ttatggcggc cgacgtcttc gcgcaccgcc agcacttccc
     6721 cgtccaggtt cacccatgcg gccgcgtgca acgcgccggt gacgcggttg agcgcctgcc
     6781 actgcggcag ttcctgcacc gccgttggca ccgccgtatg cggtagcttg agtttgttcg
     6841 gcaccggcga gggtttgcgg acagcctgca gcagggactc ctgaccgcac aatccgcagc
     6901 ccacacggcc ggtcaacgca cgccgacgtc gatccagcgt gcgatgacgc gccggatcga
     6961 tttcgatgtc gagttccatg ccttccgcga gattgcgcac ccgcacccgc cgaacgtcct
     7021 cgcgacacgc cacgatg
//
LOCUS       DATGOU010000048         9198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_5438170, whole genome shotgun sequence.
ACCESSION   DATGOU010000048 DATGOU010000000
VERSION     DATGOU010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9198
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5438170"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VKZ48_02580"
     CDS             complement(<1..193)
                     /locus_tag="VKZ48_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76773.1"
                     /translation="MGIAIGPFVFSTNSLVLLASIAVAFFWMRRESRRAGRDLERHFW
                     ITLLVSLVVARLGYVLTRLP"
     gene            complement(460..824)
                     /gene="ssrA"
                     /locus_tag="VKZ48_02585"
     tmRNA           complement(460..824)
                     /gene="ssrA"
                     /locus_tag="VKZ48_02585"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(904..1575)
                     /gene="gph"
                     /locus_tag="VKZ48_02590"
     CDS             complement(904..1575)
                     /gene="gph"
                     /locus_tag="VKZ48_02590"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549795.1"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HLU76774.1"
                     /translation="MIRAVLFDLDGTLADTAPDLAGALNRLLAERGAPPIPYEQARPV
                     TSSGARGMIKVGLGIEPDHPEYESLKDRFLEHYQAAICQDTLLFDGVPALLDELDRLA
                     IGWGIVTNKHHRFTEPLVRALGLYERCRCIVSGDTAARPKPYPDPLLHAAHAIDLAPA
                     ACLYVGDDLRDVQAARAAGMPVLAARYGYLGIGNDADPVTWEADAIIDDPREVLNYLR
                     CAPSM"
     gene            complement(1572..2273)
                     /gene="ubiG"
                     /locus_tag="VKZ48_02595"
     CDS             complement(1572..2273)
                     /gene="ubiG"
                     /locus_tag="VKZ48_02595"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HLU76775.1"
                     /translation="MQNVDQQELAKFSELAHRWWDPNSEFKPLHEINPLRLAWIEGKA
                     PLAGKRVLDVGCGGGILSEAMAGRGAEVKGIDLAEKSLKVAQLHALESGVKVEYELIS
                     AEALAEREPASYDAVTCMEMLEHVPDPASIVAACARLVKPGGDVFFSTINRNLKSYAF
                     AVVGAEYVLKLLPKGTHDYAKFIKPHELASFCREAGLDVHNLIGLTYNPLTKIYALER
                     DTDVNYMIHAVRNEQ"
     gene            complement(2358..3002)
                     /locus_tag="VKZ48_02600"
     CDS             complement(2358..3002)
                     /locus_tag="VKZ48_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLU76776.1"
                     /translation="MTKNFAKPFIVAAALLLAAVAGPANAQATDKSGYLTDTRGAVVK
                     NNYNQCWRTGYWTPALAIAECDPDLVKTAAPAPAPAPAAEPAPAPAPAPIVVPEQQKV
                     TLNADVLFDFDKSNLRPEGRQELDEVVQKMQGMELEVIIAIGHTDSIGTDEYNQKLSV
                     RRAESVKSYLVGKGIAADRIYTEGKGETQPIATNKTREGRQQNRRVEIEVVGTR"
     gene            complement(3056..4381)
                     /locus_tag="VKZ48_02605"
     CDS             complement(3056..4381)
                     /locus_tag="VKZ48_02605"
                     /inference="COORDINATES: protein motif:HMM:NF006549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HLU76777.1"
                     /translation="MSSVPRNVDRLIDAKWVVPVEPHGVVLEDHSVAIAGGDIVGIVP
                     TPEANAAYAPAERISLRQHALIPGLVNAHTHAAMSLLRGYADDKPLMVWLNEHIWPAE
                     AKHVSPQFVYDGTLLACAEMLRAGVTCANDMYFFPESAGQAFVDAGMRAAVGLIALEF
                     PSGYAADADDYIAKGLAARDRLREEPLLSFCMAPHAPYTVSDDTFARLVTIAEELDLP
                     IHLHLHETQDEIAQSLEKYGARPFDRMHGLGLVTPRLIAVHGVHLNAREIETLAQNGG
                     SVVHCPASNLKLASGFAPAASMHEAGVNLALGTDGAASNNRLDILADMRLAALLAKAV
                     SQDAAALPAHLALRMATLNGARALGLHQRIGSIEIGKAADMVAIRLDAPEVSPCYDAV
                     SQIVYSAGREHVSHVWVAGRLLVQDGTLVYFEPGALNKTVAVWNNKLLN"
     gene            4559..7231
                     /gene="gyrA"
                     /locus_tag="VKZ48_02610"
     CDS             4559..7231
                     /gene="gyrA"
                     /locus_tag="VKZ48_02610"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HLU76778.1"
                     /translation="MEPFAKETLPVSLEEEMRRSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMQESNNVWNRPYVKCARVVGEVLGKYHPHGDSATYEALVRMAQDFSMRYQL
                     IDGQGNFGSIDGDSAAAYRYTECRLRRISSELLADIDRETVDFAPNYDGKEQEPVVLP
                     TRVPNLLINGSSGIAVGMATNIPPHNLCEVVDACLALLNNPATTIDELIQIIPAPDFP
                     TAGLIYGVSGVHEGYRTGRGRVVMRARTHIEEVDKNGRQAIVVDELPYQVNKKTLLER
                     IAELVNDKKIEGISHIQDESDKSGMRIVIELKRGEMPEVVLNNLYKHTQLQDTFGINM
                     VALIEGQPRLLNLKQMLEAFIQHRREVVTRRTVFDLRKARERAHIQEGLAVALSNVDE
                     IIALIKAAQTPAEAKQKLLGKPWRSQLVQDMLARAGAEATKPETLAPGLGMTPDGYRL
                     SEAQAQRILEMQLQRLTGLEQDKILNDYRELLDTIADLIDVLAKPERVTAIIVDELNS
                     IRSQYGDARRSEIVLNTEDINIEDLITPTDLVVTFSHAGYVKSQPVADYRAQRRGGRG
                     RQATSMKEDDFIERLFIANTHDYILCFSSRGRVYWLRGYEVPQGTRTSRGKPIVNLFP
                     LEDGERINALLPIKEFDENQYVFMATAHGTVKKTPLSDFSRPREKGIIAVDLDEGDYL
                     VGALITDGTYDVMLFSDAGKAVRFHESDVRPMGRAARGVRGMRLGEGQQVISLLVAAD
                     EEQTVLTATEHGYGKRTPISEYTRHGRGTQGIIAIQTTERNGKLVGAVLVHETDELML
                     VSTGGVLIRTEVSQIREMGRSTQGVSLINLDEGTTLAGIEKVMERDDENGTDDDGSDV
                     VQIEPGSALPPTGEGEAGTELPPEDGEEGEGDGQ"
     gene            7242..8327
                     /gene="serC"
                     /locus_tag="VKZ48_02615"
     CDS             7242..8327
                     /gene="serC"
                     /locus_tag="VKZ48_02615"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005671953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HLU76779.1"
                     /translation="MTRVFNFSAGPAALPEPVLRQAAAEMLDWRGSGMSVMEMSHRGK
                     EFVSIAERAEADLRELLAVPDDYAVLFLQGGATLQFSAVPLNLMPAGGSADYVDTGQW
                     SGKAIKEAKRYGNVRVVASSKDRNYSYAPAQDTWQPIDPKAAYVHYTSNETIGGVEFH
                     WVPEAGGVPLVADMSSHILAGPIDVSKYGVIYAGAQKNIGPAGLTLVIVRKDLLGRAR
                     GDTPTLLDYKVQTEAQSMSNTPPTYAIYIAGLVFEWLKEQGGLDAIAAHNRRKAALLY
                     DLLDASAFYHSPVRREDRSLMNVPFTLADEGLDDAFLKGAKERGLVQLKGHRSVGGMR
                     ASIYNAMPIEGVQRLVDYMREFEKERA"
     gene            8324..>9198
                     /locus_tag="VKZ48_02620"
     CDS             8324..>9198
                     /locus_tag="VKZ48_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HLU76780.1"
                     /translation="MTTPHEILVLNQISTVGLKRFPAELYRVGKDVTEPDAILVRSAD
                     MHAMEIPSSVKAIGRAGAGTNNIPVKQMSARGVPVFNAPGANANAVKELVLAGLLIGA
                     RNLAPAMRFVEQLHDEEGLETRVEDGKKQFAGMELAHHTLGIVGLGKIGSLVADVALK
                     LGMQVIGYDPYITVDAAWTLPSQVKRAHSIADVLRASHFVSLHVPLAPETRGIVDAAA
                     LDAARQGLVLLNFSREAIVDENAVRAALDARKLKYYISDFPSATLLGHPCVIALPHLG
                     ASTREAEENCAIMVV"
BASE COUNT         1691 a         2861 c         3017 g         1629 t
ORIGIN      
        1 gcggcaggcg ggtcaggacg tagccgaggc gggcgacgac gagactgacg agcagcgtga
       61 tccaaaagtg ccgttccaga tcgcggcccg cgcgtcggct ttcccggcgc atccagaaga
      121 atgcgaccgc gatcgacgcc agaagcacca ggctgttcgt ggaaaaaacg aaaggtccga
      181 ttgcaatgcc catgcggcga gtttagcggg gcggtgtcgc tgaattgatc tttgcggcgg
      241 ggaccgcgac ggcgggaatt gtggtcgtct cgcatggcat ctgcgatccg accatccgtc
      301 gccgcatgcg gctcgggacc gccgctgcgc tcaggcgcat cggcgtccaa cctgaagtcc
      361 cccggacttc gggttgtgaa cccgaacgcg ggttcgcatc cgcaccgcgt tcagacgcta
      421 caaaagcaaa tggccccgga tggggccatt tgcttttggt ggtggaggcg gcgggaatcg
      481 aacccgcgtc cgcaagtcct ctacaggcag ttctacatac ttagccttgc cgtttgattt
      541 tgacccggaa cccgccgaca ggcaggctgg ttacgagcga gtcaccttgg atttaacgcc
      601 gtacgaagtg accccgcacg acgcgatttc atgtgaatga cccgactcta gcttgcgcta
      661 gctaccccat gaacaaggta gtgccgggct cgcgggttat taagccgcga gagcgaaacg
      721 ttcgtcgttc gcagttattg cgtttccaga tggatttacg aggtaactgg tcctcggtat
      781 gccctgcgct gcttcgctac ccacgtcgaa accaggtcgc ccccggaatc ctgtgttgct
      841 gctacagcgg tgcgattatc acacggttcc accatctcca tcctgccgtg tgccttccgc
      901 acgctacata gatggggcgc aacgcaaata gttcaatacc tcgcgcgggt cgtcgatgat
      961 ggcgtccgct tcccaggtga cggggtcggc gtcgtttccg atgcccagat aaccgtagcg
     1021 ggccgccagc accggcatgc cggcggcgcg cgccgcctgg acgtcgcgca aatcgtcgcc
     1081 gacatagagg caggcggccg gggcgagatc gatcgcgtgc gcggcgtgca gcagcgggtc
     1141 gggatagggc ttgggccgcg cggcggtgtc gccggacacg atgcagcggc aacgctcgta
     1201 gagacccagc gcgcgcacca gcggctcagt gaagcggtgg tgcttgttgg tgacgatgcc
     1261 ccagccgatg gcgagccggt cgagttcgtc cagcagcgcc ggtacgccgt cgaagagaag
     1321 ggtgtcctgg cagatggccg cctggtagtg ctccagaaag cgatccttga gcgactcgta
     1381 ttcggggtga tccggctcga tgccgaggcc gaccttgatc atgccgcgtg cgcccgagga
     1441 cgttacgggc cgcgcctgct cgtagggaat gggcggcgcg ccgcgttcgg ccagcaggcg
     1501 gttcagggcg ccggccagat cgggtgcggt gtcggccagc gtgccgtcca gatcgaacag
     1561 taccgcgcgg atcattgttc gttgcggacc gcgtggatca tgtagttcac gtcggtgtcc
     1621 cgttccagcg catagatctt ggtgagcggg ttgtaggtca gcccgatgag gttgtgcacg
     1681 tcgagccccg cttcgcggca gaagctggcc aactcgtgtg gcttgatgaa cttcgcgtag
     1741 tcgtgcgtcc ccttgggcag cagcttgagc acgtattcag cgcccaccac cgcgaaggcg
     1801 taggacttga gattgcgatt gatggtggag aagaacacgt cgccgcctgg tttcaccaga
     1861 cgcgcgcagg ccgccacgat gctcgccgga tcgggcacgt gctcgagcat ttccatgcag
     1921 gtgacggcgt cgtacgacgc aggctcgcgt tcggcgagcg cttctgcgga aatcagttcg
     1981 tattcgacct tcacgccgct ttccagtgca tgcagttgcg cgaccttgag cgatttctcc
     2041 gccagatcga tgcctttgac ctcggcgccg cgaccggcca tcgcctcgga gaggatgccg
     2101 ccgccgcagc ccacgtcgag cacgcgtttg cctgcgagcg gtgccttgcc ctcgatccac
     2161 gcgaggcgca gcggattgat ttcgtgcagc ggtttgaact cgctgttcgg atcccaccag
     2221 cgatgcgcca gttcgctgaa tttggccagt tcctgttgat cgacgttctg catgcttcaa
     2281 aacaacgttt agggaaggcg tatcttaagc cataaaaaaa gccccgccgg agcggggctt
     2341 ttgcgaagcg ataacgctta gcgcgtaccc accacttcga tttccacgcg gcggttctgc
     2401 tgacgacctt cgcgggtctt gttggtggcg atcggctgcg tttcgccctt gccttcggtg
     2461 tagatgcgat ctgcggcaat gcccttgccg accaggtagc tcttcaccga ctcggcgcga
     2521 cgcacggaca gtttctggtt gtactcgtcg gtgccgatgc tgtcggtgtg accgatggcg
     2581 atgatcacct cgagttccat gccctgcatt ttctgcacga cttcatcgag ttcctgacgg
     2641 ccttccggac gcaggttgga cttgtcgaag tcgaacagca cgtcggcatt gagcgtgact
     2701 ttctgttgct cgggcacgac gatcggcgcc ggagcgggcg caggtgcggg ttccgctgcg
     2761 ggtgcgggcg caggtgccgg ggctgcggtc ttcaccagat ccggatcgca ctcggcgatg
     2821 gcaagggccg gggtccagta gccggtgcgc cagcactggt tgtagttgtt cttgaccacg
     2881 gcaccgcggg tatcggtgag ataacccgac ttgtcggtcg cctgggcatt ggcggggccg
     2941 gccaccgcgg cgagcagcaa cgccgccgca acgatgaaag gtttagcaaa gttcttagtc
     3001 atgttgtttc ctcatagaaa aacgcgcgtt tcgcggctgt tgagttgaaa atcagtcagt
     3061 taagtaattt gttgttccaa accgcaaccg tcttgttcag cgcccccggc tcgaaataca
     3121 cgagggtccc gtcctgaaca agcaatcttc cggccaccca cacatgcgag acgtgttcgc
     3181 ggcctgcgct atagacgatc tgcgatacgg cgtcgtagca gggcgacact tccggggcgt
     3241 cgagccggat ggccaccatg tctgcggctt tgccgatttc gatggacccg atgcgttgat
     3301 gcaagccgag tgcacgggcg ccgttgagcg tggccatccg cagtgcaagg tgggcgggga
     3361 gcgccgccgc atcctgcgac accgccttgg cgagcaaggc cgccaggcgc atgtcggcca
     3421 ggatgtccag ccggttgttg ctggccgccc catcggtgcc gagtgcgaga ttgacgcctg
     3481 cttcgtgcat ggaggcagcc ggggcgaagc cgcttgcgag tttgagattg gatgcggggc
     3541 agtgtacgac cgagcccccg ttctgcgcca gcgtttcgat ttcccgtgca ttgagatgca
     3601 cgccgtgcac cgcgatcaac cgcggcgtca ccagtcccag tccgtgcatg cggtcgaagg
     3661 gccgcgcgcc gtacttttcc aggctctgcg cgatttcgtc ctgcgtttcg tgcagatgca
     3721 ggtgaatcgg cagatcgagt tcctcggcga tggtgaccag ccgggcgaag gtgtcgtcgc
     3781 tcaccgtata gggcgcatgg ggcgccatac agaaggacag cagcggctcc tcgcgcaacc
     3841 ggtcccgcgc cgccagcccc ttggcaatgt agtcgtcggc gtcggcggcg tagccgctgg
     3901 gaaattccag cgcgatcagg ccgaccgccg cacgcatccc cgcatcgacg aacgcctgcc
     3961 ccgcggactc ggggaagaaa tacatgtcgt tggcgcaggt cacgccggca cgcaacatct
     4021 ccgcgcaggc gagcagggtg ccgtcgtaca cgaactgcgg cgacacgtgc ttcgcttcgg
     4081 cgggccagat gtgttcgttg agccacacca tcagcggctt gtcgtccgcg tagccgcgca
     4141 gcaggctcat ggcggcatgg gtgtgggcgt tgaccagacc cgggatcagc gcgtgctgcc
     4201 ggagcgagat gcgttccgcc ggggcgtatg cggcgttcgc ctcgggcgta ggcacgatgc
     4261 cgacgatgtc gccgccggcg atggcaacgc tgtggtcctc cagcacaacc ccatgcggtt
     4321 cgaccggaac gacccatttc gcgtcgatca gccgatcgac gttgcgggga acggagctca
     4381 tggggcggca caatagcaaa aaaaatgaat ccgtcactga ttcgggcaaa tagatggcgg
     4441 aacccggttt gccgtgtggc gccgctacag attggccgtc aaaagcggcg gatgctagaa
     4501 ttcagccctt tttaccgaca cttagcgccg cccgatcaag ggcgacagca agccccgcat
     4561 ggaaccgttc gccaaagaga cccttccggt cagcctagaa gaagaaatgc gacgctctta
     4621 cctcgattac gccatgagcg tgatcgtggg acgggcgctg cctgatgtac gcgacggatt
     4681 gaaacccgtg caccgacgag tgctgttcgc catgcaggag tcgaacaacg tctggaaccg
     4741 gccttatgtg aagtgcgccc gggtggtcgg tgaggtgctc ggtaaatacc acccgcacgg
     4801 cgactcggcc acttacgagg cgctggtgcg gatggcgcag gatttctcca tgcgctacca
     4861 gctgatcgac ggccagggca acttcggctc catcgacggc gactccgcgg cggcctatcg
     4921 ctacaccgag tgccggctgc ggcgcatcag ttccgaactg ctggcggaca tcgatcgcga
     4981 aacggtcgat ttcgcgccca actacgacgg caaggagcag gagcccgtcg tactgcccac
     5041 gcgcgtgccg aacctgctca tcaacggttc gtccggtatc gccgtcggca tggcgaccaa
     5101 catcccgccg cacaacctgt gcgaagtcgt cgatgcctgc ctggcgctgt tgaacaaccc
     5161 cgccacgacc atcgatgaac tgatccagat cattccggcg ccggactttc cgacggcggg
     5221 tctgatctac ggcgtaagcg gcgtgcatga gggctatcgc accggtcgcg gccgcgtggt
     5281 catgcgtgcg cgcacgcaca tcgaagaggt cgacaagaac ggccggcagg cgatcgtcgt
     5341 cgacgagttg ccctatcagg tgaacaagaa gaccttgctc gagcgcatcg ccgagctggt
     5401 caacgacaag aagatcgaag gcatttcgca tatccaggac gagtcggaca agtccggcat
     5461 gcgcatcgtc atcgagttga agcgcggcga gatgcccgag gtggtgctga acaacctgta
     5521 caagcacacg caactgcagg acaccttcgg catcaacatg gtcgcgctca tcgaaggcca
     5581 gccgcgtctg ctcaacctca agcagatgct cgaggccttc atccagcacc ggcgcgaagt
     5641 ggtcacgcgg cgcacggtgt tcgatctgcg caaggcgcgg gagcgtgcgc acatccagga
     5701 aggcttggcg gttgcgctgt cgaacgtcga cgaaatcatc gcgctcatca aggccgcgca
     5761 aacgcctgcc gaagccaagc agaagctgct cggcaagccg tggcgttcgc aactggtgca
     5821 ggacatgctg gcacgggccg gcgccgaggc gaccaagccc gagacactcg cgcccggact
     5881 gggcatgacg cccgacggct accggctgtc ggaagcgcag gcgcagcgca ttttggaaat
     5941 gcagttgcag cgcctcaccg gtcttgagca ggacaagatc ctcaacgact accgcgagtt
     6001 gctggacacc atcgccgacc tcatcgacgt gctcgccaag cccgagcgcg tgacggcgat
     6061 catcgtcgac gaactgaata gcatccgctc gcagtacggc gatgcgcgcc gcagcgagat
     6121 cgtcctcaac accgaggaca tcaacatcga ggatctcatc acgccgaccg atctggtggt
     6181 gaccttctcg catgccggct acgtcaaatc gcagccggtg gccgactacc gcgcacagcg
     6241 ccgcggcggc cgcggccgtc aggcgacgtc gatgaaggaa gacgatttca tcgagcgcct
     6301 gttcatcgcc aacactcacg attacatcct gtgcttctcc agccgcggcc gcgtgtactg
     6361 gctgcgcggc tacgaggtgc cgcagggtac gcgcaccagt cgcggcaagc cgatcgtcaa
     6421 cctgtttccg ctggaagatg gcgagcgcat caatgcgctg ctgccgatca aggagttcga
     6481 cgagaaccag tatgtcttca tggcgaccgc acacggcacg gtgaagaaaa ccccgctgtc
     6541 ggatttttcg cgtccgcgcg agaagggcat catcgccgtc gatctggacg agggcgacta
     6601 cctcgtcggc gcgctgatca ccgacggcac ctacgacgtg atgttgttct ccgacgccgg
     6661 caaggcggtg cgtttccacg aaagcgacgt gcgccccatg ggccgggcgg cacgcggcgt
     6721 gcgcggcatg cgtctgggcg aagggcagca ggtcatttcg ctgctggtcg ccgcggatga
     6781 agaacagacc gtgctgacgg cgacggaaca cgggtacggc aaacgcacgc cgatttcgga
     6841 atacacgcgc cacggtcgcg gcacgcaggg catcatcgcc atccagacca cggagcggaa
     6901 cggcaaactt gtcggcgccg tgctggtgca cgagaccgat gaactgatgc tggtctcgac
     6961 cggcggcgtg ctgatccgca cggaagtgtc gcagattcgc gaaatgggcc gctccaccca
     7021 gggtgtcagc ctgatcaacc tggacgaagg caccacgctt gccgggatcg agaaggtgat
     7081 ggagcgcgac gacgaaaacg gtaccgacga cgacggtagc gatgtggttc agatcgagcc
     7141 gggctctgca ctgccgccca cgggcgaagg cgaggccggc acggaattgc cgcccgagga
     7201 cggcgaggaa ggcgagggcg acgggcagtg aggacggcgt cgtgacgcgc gtgttcaatt
     7261 tcagcgccgg accggccgcg ttgccggagc cggtgctgcg gcaggccgcc gccgaaatgc
     7321 tcgactggcg cggcagcggc atgtcggtga tggaaatgag tcaccgcggc aaggagttcg
     7381 tcagcattgc cgagcgcgcc gaagccgatc tgcgtgaact gctggcggtg ccggacgatt
     7441 acgccgtgct gtttttgcaa ggcggtgcca cgctgcagtt ttccgccgtg ccgctcaacc
     7501 tcatgcccgc gggcggttcc gccgattacg tcgataccgg gcagtggtcg ggcaaggcga
     7561 tcaaggaggc gaagcgctac ggcaatgtgc gcgtagtcgc ctcgtccaag gacaggaact
     7621 acagctacgc gccggcgcag gacacctggc agcccatcga tccgaaggcc gcctacgtgc
     7681 actacacgtc gaacgagacc atcggtggcg tcgagttcca ttgggtgccc gaggcgggcg
     7741 gggtgccgct ggtggcggac atgtcctcgc acatcctcgc cggcccgatc gacgtgtcga
     7801 aatacggcgt gatctacgcc ggcgcgcaga agaacatcgg tcccgcgggg ctgacgctgg
     7861 tgatcgtacg caaggatctg ctgggccgtg cgcgcggcga cacgccgacg ctgctcgact
     7921 acaaggttca gaccgaagcg cagtccatgt ccaacacccc gccgacctat gcgatctaca
     7981 tcgccgggct ggtgttcgaa tggctgaaag agcagggcgg cctcgatgcc atagccgcgc
     8041 acaatcgcag gaaggcggcg ctgctctacg atctgctgga tgcgtcggcc ttctatcaca
     8101 gtccggtgcg gcgtgaagac cgctcgctca tgaatgtgcc gttcacgctg gccgatgaag
     8161 ggctcgacga tgcgttcctc aagggcgcga aagaacgcgg tcttgtccag ttgaagggcc
     8221 atcgttcggt cggcggcatg cgtgcctcca tctacaacgc catgcccatc gaaggcgtgc
     8281 agcggctggt cgactatatg cgcgagttcg agaaggagcg ggcatgacga cgccgcatga
     8341 gatcctggtg ctgaaccaga tttccaccgt cggcctgaaa cgcttcccgg ccgagctcta
     8401 tcgcgtcggc aaggacgtga ccgaacccga tgcgatactg gtgcgctcgg ccgacatgca
     8461 cgccatggag attccctcca gtgtgaaggc catcggccgc gccggcgcgg gcaccaacaa
     8521 cattccggtg aagcagatga gcgcgcgggg cgtgcccgtg ttcaacgcac cgggcgccaa
     8581 cgccaatgcg gtgaaggaac tggtgctcgc cggcctgctg atcggcgcgc gcaatctcgc
     8641 gccggccatg cgcttcgtcg aacagttgca cgacgaagag gggctggaga cgcgcgtcga
     8701 agacggcaag aagcagttcg ccggcatgga actcgcgcat cacacgctgg gcatcgtcgg
     8761 gctgggcaag atcggcagcc tggttgcgga cgtcgcgctc aagctcggca tgcaggtcat
     8821 cggctacgac ccgtacatca ccgtggatgc agcgtggacg ctgccttcgc aggtgaagcg
     8881 cgctcattcc atcgccgacg tgctgcgcgc cagccatttc gtcagtctgc acgttccgct
     8941 ggcgccggag acgcgcggca tcgtcgatgc cgccgccctg gacgccgcgc gccagggcct
     9001 cgtgctgctc aacttttcgc gcgaggcgat cgtcgacgaa aacgccgtgc gcgccgcgct
     9061 ggacgcacgc aagctgaagt actacatcag cgatttccct tcggccacct tgctcggcca
     9121 cccctgcgtg atcgcgctgc cgcatctggg cgcctccacg cgcgaggcgg aggagaactg
     9181 cgcgatcatg gtcgtcga
//
LOCUS       DATGOU010000049        14100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3229679, whole genome shotgun sequence.
ACCESSION   DATGOU010000049 DATGOU010000000
VERSION     DATGOU010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14100
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3229679"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..393)
                     /locus_tag="VKZ48_02625"
     CDS             complement(256..393)
                     /locus_tag="VKZ48_02625"
                     /inference="COORDINATES: protein motif:HMM:NF022103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HLU76781.1"
                     /translation="MRRPTQPTRINSRLLLGDSGELVIEHAGREYRLRVTQNGKLILT
                     A"
     gene            624..812
                     /locus_tag="VKZ48_02630"
     CDS             624..812
                     /locus_tag="VKZ48_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HLU76782.1"
                     /translation="MYVCLCNAVTDREIRQCADLGACSLEDLGDAMGVATCCGNCADT
                     AERVLQEHLASAALPKAA"
     gene            965..1435
                     /gene="bfr"
                     /locus_tag="VKZ48_02635"
     CDS             965..1435
                     /gene="bfr"
                     /locus_tag="VKZ48_02635"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HLU76783.1"
                     /translation="MKGDPRVIQYLNQALKNELTAINQYFLHARMFENWGFTKLYKHE
                     YEESIDEMKHADALIKRILFLEGLPNLQDLGKLLIGENVREALSCDLQLEHAGHALLK
                     EAIAYCESVNDYVSRELFEDILESEEGHIDWIETQLGLIDSVGLQNYLQSQMDP"
     gene            1527..2489
                     /locus_tag="VKZ48_02640"
     CDS             1527..2489
                     /locus_tag="VKZ48_02640"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HLU76784.1"
                     /translation="MSQAVFALLRRLHAGGLHPREELVRALGVSQGELDDCIAALRAQ
                     GVAIESTPAGCALRRRVDLLDATALSERLHERDTDLRVTLYDECDSTNARLLAEPEVH
                     ARVICCELQTAGRGRRGRVWRSGLGDALTFSLGWRIDRPVHALGGLSLAAAVTCAGTL
                     EALGIGDVQVKWPNDLMRSGGKLGGILIEVARSTPTYCDVVIGVGLNVRFDAHVRAGI
                     DQPVADLADAQTMPSRTLLLAQLLHDLASGLQRYAAHGFPDFKREWLRLHAFQDRPVR
                     LAMNEYDSIEGTARDIADDGALIVATAAGVRHFHAGEITLRGVA"
     gene            2486..3214
                     /locus_tag="VKZ48_02645"
     CDS             2486..3214
                     /locus_tag="VKZ48_02645"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HLU76785.1"
                     /translation="MILAVDAGNTRIKWGLRDAAGQWITRGAAAVDELERAYEEWARM
                     PVPTRIAIANVAGPAVRETVVDLIGRWSVEPVWLETTARACGVVNGYEIPTQLGVDRW
                     AAAVAAWLRQGRECLVVMAGTATTVDVVDADGVFRGGLILPGLGLMKRALVDHTAALK
                     FAPGVYRDLPRNTADAIESGCIHAQIGAIERMRRRLSPDAGCVISGGGAPALVPHLEA
                     PVMMAENLVLEGVVALADAVAQAG"
     gene            3234..3644
                     /locus_tag="VKZ48_02650"
     CDS             3234..3644
                     /locus_tag="VKZ48_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76786.1"
                     /translation="MRIVVLILLLANVALFAYLEYDAQAERDVSRAMAQQVQPERIRL
                     MTEQEVAALQPGAAPCLEVGPLSSADLSAARDAFAAAQAALRMTERTDASSGEVYLQL
                     RDVPGAARERVNGIAARFGGAALRVCPAPETASG"
     gene            complement(3761..4258)
                     /gene="rfaE2"
                     /locus_tag="VKZ48_02655"
     CDS             complement(3761..4258)
                     /gene="rfaE2"
                     /locus_tag="VKZ48_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HLU76787.1"
                     /translation="MQSNDYPRPSFETKLCTPDELPARAAALARPLVFTNGVFDILHR
                     GHVTYLAQARALGASLVVALNSDASVKRLGKGEDRPINTLADRMAVMAALECVDLVTW
                     FEEDTPLAAILACRPEVLVKGGDWQPDAIVGAEEVRGWGGTVHAIPFEHQRSTTALLG
                     RIRGA"
     gene            complement(4284..5123)
                     /locus_tag="VKZ48_02660"
     CDS             complement(4284..5123)
                     /locus_tag="VKZ48_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HLU76788.1"
                     /translation="MLYPEIFKSLERVRWDMGKDVPWDQFDGSKLSDDQAHTIKMNAI
                     TEWAALPATEMFLRDNAHDSDFSAFMSVWFFEEQKHSLVLMEYLRRFRPELAPTEEEL
                     HAVRFQFDPAPPLETLMLHFCGEIRLNHWYRRASEWHTEPVIKKIYEILSHDEARHGG
                     VYRQYMKRAIERCGDEARRAFAKIGMLMASSGRSSKPLHPTNLHVNKELYPQDTVQSK
                     LPDPDWLEHWLDEQIKFDKVWEGKVVTAILRNCSILFEQTIETVQELNRYRKGLGAAP
                     AAV"
     gene            complement(5238..6197)
                     /locus_tag="VKZ48_02665"
     CDS             complement(5238..6197)
                     /locus_tag="VKZ48_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HLU76789.1"
                     /translation="MNKLGTTMQDGNYAQRWRLSIGAVERDTGLSKDTLRVWEKRYGF
                     PQPERDKFGERVYPQEQVEKLRLLKRLIDRGHRPGKLVSRSVTELLQLVDPQPARAIA
                     SGAATPTQPFAAFNTQDVAEIKQQLRDRLASEGLRDFVLQTVVDLHQQMSDAWMRGQL
                     ALYEERLYTEALLSTLQGGLGAIPLQNRTPRVLLGTFPNESRTLGLTMMECLLASEGA
                     SCLSLGAQAPVVDMMLAARAQQADIVALYFSATYPITQALEGLGALREQLPESAQVWI
                     GSSNPAIMRRAPADVVALPRFDMIPEAVASWRQSQSVGAFSTH"
     gene            6633..9245
                     /locus_tag="VKZ48_02670"
     CDS             6633..9245
                     /locus_tag="VKZ48_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HLU76790.1"
                     /translation="MLTRRDIRPDAKTEARTTRADPAFNYFQHTIAELEQRLAHLSEA
                     QRALRISEERYRALYDSNPVMSFTADTDGTVLSVNAYGAQRLGYEPEELIGRSVFDLA
                     FAADRRAVRQAFADCLREAGHVHSGEFRKRRRDGQVVWVRETLRAVQEPGGALVVLLV
                     CEEAGVSHAVESRTREAAVRLELALAGANAAMFNWDIDSGAVHLSERWREMLGDLPRE
                     TVTTIRALSDVVHADDIRQFRERVLDAVKGITPALEYEFRARARNGDWRWIITRGKVV
                     ERGADGRAVRMIGTNTDVTALRQAEEALRRAEARLRSLTELSSDWYWEQDETLRYTHV
                     TGGMLAQTGFDPRQDIGRTCWELEGVEGGEPHWPDHRRALARRQPFHDFVYKRRNLAG
                     ELRYHSLSGQPVYTADGRFSGYCGIGRDVTDRFKAEQRIHRLAHYDSLSGLPNRALFH
                     QRLEQALARARRFGRTLAVLSLDLDRFKSINDSFCHSAGDQVLCEVAERLTRATGATD
                     TVARLSGDEFVVLLESCESAAEVSAFARSVLATLGRPYTVAGQECHLTASMGISLYPQ
                     DGGDGLALLRDADTALNRAKEKGKNCFQFYSARMNAQAYERLALESKLRRALEHEEFV
                     LHFQPTVELGSGRIVSVEALARWPQADGSMIEPATFIPVAEDSGLIEVLGQWVLRKAC
                     VQAKAWRAAGLPPIRVAVNLSARQFLQEDLLAQVAAVIAETGIEPALLEFEITESLMF
                     ANPQHAAGLLHALKLMGVRLALDDFGTGYSSLSYLKRFPFDSVKIDRSFVRDLPQDND
                     DAAITRAVIAMSHSLRMTVTAEGVESAAQLAFLRECGCDAAQGNHFSPALPADRFAAL
                     FDRDLRTQPIRLVR"
     gene            complement(9292..9879)
                     /locus_tag="VKZ48_02675"
     CDS             complement(9292..9879)
                     /locus_tag="VKZ48_02675"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HLU76791.1"
                     /translation="MNRRLAAAVLIFCASVLTAGCFPLVVGGTAGGAALIADRRPPDI
                     VATDQRIEWTMSREIEKRLGNQVHINVTSYNRQVLLTGEVTTPEGKAVIDDVASRVHD
                     VRKVYNEVQIGLPSSLDSRSRDTYITSQVKTRMLNQQKFNPLHVKVVTEARVVYLMGL
                     VTVEEAEEATEIARSTSGVQRVVRLFEYVQLQPSK"
     gene            complement(9884..10474)
                     /locus_tag="VKZ48_02680"
     CDS             complement(9884..10474)
                     /locus_tag="VKZ48_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008921248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HLU76792.1"
                     /translation="MDLPARIRQQFNDSAQTKLDAVDALTAPIAEAVDAMVKCLLANG
                     KILACGNGGSAADAQHFSAELLNRFERERPGLAGIALTTDTSTLTSIANDYDYNQVFS
                     KQVLALGQPNDVLLAISTSGNSPNVVAAIHAAHEREMRVVALTGKSGGEMAKWLTPND
                     VHICVPHNRTARIQEVHLLTLHCLCDGIDCLLLGTE"
     gene            complement(10570..10956)
                     /locus_tag="VKZ48_02685"
     CDS             complement(10570..10956)
                     /locus_tag="VKZ48_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HLU76793.1"
                     /translation="MARHLPVRRADRTGVKASDGQAAEHLAAAWLERRGLRILARNYR
                     CRTGEIDLIAQDGDTLVFVEVRLRRSAAFGGAAASVHGAKQRRLLSAARHYLMDRGEQ
                     PCRFDVVVLDALDEARIEWLRDAFGA"
     gene            complement(10911..12113)
                     /locus_tag="VKZ48_02690"
     CDS             complement(10911..12113)
                     /locus_tag="VKZ48_02690"
                     /inference="COORDINATES: protein motif:HMM:NF016251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HLU76794.1"
                     /translation="MPPHSLIRSLCAALLAASLALSCATQPPATAPQPTAPAAQPELQ
                     ITPDGQPVLPGEPLPETPITPGTEPEQGVVPPLPDMSQRAPIMTLLLPLDSPDFRRAA
                     YPFVRGVEAAALLDPAPLPIEVRATDASPEGIYQAYLEVLQQVPAVIVGPMTRSGVSA
                     IAASGVVMVPTLTLNQPDNEQPLPPGLYAFGLSVEDEARMLAQRAFRDGMRRIGAVST
                     PTPLSHRSREAFLDEWRKLGGEAVASVDASPASDLTGMRTMLTELNLDAVYFAAQYQD
                     ARLIRPYLPSQVPVYATSQINSFPIDPVGMVDLNGVRFVDMPWLLAPEHESFVNFPRP
                     ADLSGGALRFYALGVDAYRIATQLAQGRQDIRLDGLTGHISVQPNGSVERRPWLAIFL
                     SGEQIVLE"
     gene            12126..12980
                     /gene="rsmI"
                     /locus_tag="VKZ48_02695"
     CDS             12126..12980
                     /gene="rsmI"
                     /locus_tag="VKZ48_02695"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011655710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HLU76795.1"
                     /translation="MKEERVALYVVATPIGNLGDITQRALDTLRDADVIYAEDTRTSA
                     ALLAHHGIRTPLKALHEHNEARATPEVLRALDAGRAVALISDAGTPAISDPGAMVVAA
                     AREAGHRVVPIPGPNAAIAALSASGMTGPFLFVGFLPPKAGARRKLLESWRSAPHALV
                     FYEAPHRVLECVDDLLQVFGPARTLTIARELTKLYETIHVCTLGEAPAWFAEDANRLR
                     GEFVLIVSGAPADEDGALADGERVLKLLLEELPLKRAARLAADITGARKNELYERGLA
                     YKDDDKDE"
     gene            12980..14011
                     /gene="pyrC"
                     /locus_tag="VKZ48_02700"
     CDS             12980..14011
                     /gene="pyrC"
                     /locus_tag="VKZ48_02700"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HLU76796.1"
                     /translation="MQSITLTRPDDWHLHLRDGAALASVLPHSARQFARAIIMPNLRP
                     PVTTVELAAAYRERILAALPAGMRFEPLMTLYLTDNTTAAEIATAKASGFVHALKYYP
                     AGATTNSDSGVTSLDRASAALAAMEEHDVPLLVHGEVTTPGVDVFDREKVFIDTVMSD
                     IVRRFPGLRVVFEHITTGDAAAFVRDSGPNIGATITAHHLLYNRNAIFQGGIRPHYYC
                     LPVLKREQHREALIAAATGESNKFFLGTDSAPHPRHDKETACGCAGCFTAHAAIELYA
                     EAFEQAGALDRLEAFASFNGADFYGLPRNEERITLVRAPWQVPRSVIYADAELTPLRA
                     GESLAWRMQ"
BASE COUNT         2420 a         4521 c         4700 g         2459 t
ORIGIN      
        1 cgggcgttcc gcaggttccg gggtaatgaa gctcaccatg atgggcgaac ccttggcctg
       61 ggccaggctg ggcggctggt gggtcagcac cagtccaagc agcgcggcat ggaagagggc
      121 cgtgaccgtg agactcgcgg cgcgagtgtg cggggggcgg ggggcggccg gtgatgccaa
      181 caacaacgtc cagtctccga atcggcgcaa gccggccacc cgtgcgggcc ggccggctgc
      241 gagcacgcgc ggtgctcagg cggtgaggat cagttttccg ttctgggtga cgcgcagtcg
      301 gtattcgcgg ccggcatgct cgatgaccag ttcgcccgaa tcacccagaa gcagcctgct
      361 gttgatgcgc gtcggttggg tcgggcgccg catgttcgcg ccggattcgg cgctcgcagg
      421 cagacggttt tgctcgaccc caatcttcat atgggcagaa cgataacgat tctcattttt
      481 ggtgtcaaga atatcgctgg caatcccctc aatggcttag tggatattga ctgtataagt
      541 cgggcgataa aaagtcgctt gcatttgcgt cccgccttgt tgataatcat tcccattcgt
      601 aacgtcccgc tgcaggtagt cccatgtacg tatgcctttg caacgccgtc accgaccggg
      661 aaatccgtca gtgcgctgat ctgggtgcct gcagcttgga agatctgggc gatgcgatgg
      721 gagtggcaac ctgctgcggc aactgcgccg acaccgccga acgcgtcttg caggaacacc
      781 tcgcctccgc ggcgctgccc aaagccgcct gagcgctctc ccgcgccact tcttcacttc
      841 actgcgcgcc gtccccaacg cggttcagtc cgtgcactga ccgtgctatc ttcgctgctg
      901 gtacggcaat catcgccgtg gcgttcaacg ccgcctcgtg cggcactcca gcgagcgagc
      961 tcccatgaaa ggcgatccgc gcgtcatcca atacctcaac caggctctca agaacgaact
     1021 caccgcgatc aatcagtact tcctgcatgc gcggatgttc gagaactggg gtttcaccaa
     1081 gctctacaag cacgagtacg aagagtccat cgacgaaatg aagcacgccg atgcactcat
     1141 caagcgcatc ctctttctcg aaggactgcc taacctgcag gacctcggca agctgctcat
     1201 cggcgagaac gtgcgcgaag cgctgtcctg tgacctgcag ctcgaacacg ccggccatgc
     1261 cctactgaaa gaggccatcg cgtattgcga gtcggtcaac gactacgttt cgcgcgagct
     1321 gttcgaagac atcctcgagt ccgaggaagg gcatatcgac tggatcgaaa cccaactcgg
     1381 cctcatcgac agcgtcggac tgcagaacta tctgcagtcg cagatggatc cctgattccg
     1441 gcccgccccg ccccgcgttt cgcggggcgc ctggcagggc gtgctaccat cgcgcgcctc
     1501 gcttcgttct cccgtttcat ctccccgtgt ctcaagcggt tttcgcatta ctgcgcaggc
     1561 tccatgcggg cgggttgcac ccgcgcgagg aactggtgcg cgcgctgggc gtatcccagg
     1621 gcgaattgga cgactgcatc gccgcgctgc gtgcgcaagg cgtggcaatc gaatccaccc
     1681 ctgccggctg cgcgttgcgc cgtcgcgtcg acctgctcga cgcaacggca ctgagcgaac
     1741 ggctgcacga gcgcgacacg gatctgcgcg tcacgcttta cgacgaatgc gattccacca
     1801 atgcccgcct gcttgccgaa cccgaggtgc acgcacgcgt catctgctgc gaattgcaga
     1861 ctgccggtcg cggccggcgg ggtcgcgtct ggcgctcggg tctgggggat gcgctcacgt
     1921 tcagtctcgg ttggcgcatc gatcggccgg tgcatgcgct gggcggattg tcgctggcgg
     1981 ccgccgtcac ctgtgccggc accctcgagg cgctcggtat cggcgacgtg caagtgaagt
     2041 ggccgaacga cctgatgcga agcggcggca agctgggcgg catcctgatc gaagtggcgc
     2101 gcagcacgcc gacgtattgc gatgtggtga tcggcgtcgg cctgaacgtg cgcttcgacg
     2161 cgcatgtgcg agcaggcatc gatcaaccgg tggcggatct ggccgacgcg cagacgatgc
     2221 cctcgcgcac gctgctgctg gcgcagttgc tgcatgatct ggcgagcggc ctgcaacgtt
     2281 acgctgcgca cggctttccg gacttcaagc gcgagtggct gcgcctgcat gcttttcagg
     2341 atcggccggt gcgtctggca atgaacgaat acgacagtat cgagggcacc gcgcgcgaca
     2401 tcgccgacga cggcgcattg atcgtcgcga ccgcagcggg cgtgcggcac ttccacgccg
     2461 gcgaaattac cttgcgcggg gttgcatgat tctcgccgtc gacgccggaa acacccgcat
     2521 caaatggggc ctgcgcgatg ccgcgggcca atggatcacg cgcggcgctg ccgcggtgga
     2581 cgaactcgag cgcgcttacg aggagtgggc gcgcatgccg gtgcccacgc gcatcgccat
     2641 cgccaacgtg gccgggccgg cggtccgcga aacggtcgtc gatttaatcg gacgctggtc
     2701 ggtcgagccc gtttggctgg aaacgacggc gcgcgcctgc ggcgtcgtca acggctacga
     2761 aatcccgacg caactagggg tcgatcgctg ggccgcggcg gtggccgcct ggttgcgtca
     2821 gggccgcgaa tgtctggtgg tgatggccgg gaccgcgacc accgtcgatg tcgtcgatgc
     2881 cgacggcgtt ttccgcggcg gcctgatcct gcccgggctc ggattgatga agcgcgcgct
     2941 cgtcgatcac accgccgcgc tgaaattcgc gcccggcgtt tatcgcgact tgccgcgcaa
     3001 cacggccgac gcgatcgaaa gcggctgcat tcacgcacag atcggcgcca tcgagcgcat
     3061 gcgccgacgc ctgtcgccgg atgccggttg cgtgatctcc ggtgggggcg cgcctgcgct
     3121 ggtgccgcac ctcgaagcgc cggtcatgat ggcggagaac ctggtgctgg aaggcgtggt
     3181 ggcgctggcc gatgcggtcg cgcaggccgg ctgagattca acggggagca tcgatgcgta
     3241 tcgtcgttct gatcctgctg ttggccaacg tggccctgtt cgcctacctc gagtacgacg
     3301 cccaggccga gcgcgatgtc tccagggcga tggcgcaaca ggtgcagccg gagcgcatcc
     3361 ggctgatgac cgaacaggaa gtggcggcgc tgcagccggg cgcggcgccg tgtctggaag
     3421 tcggtccgct gagttcagcc gacctgagtg ccgcacgcga cgctttcgcc gccgcgcaag
     3481 cggcgctgcg gatgacggag cggacggatg cgagcagcgg tgaggtgtat ctgcaactgc
     3541 gcgacgtgcc gggggctgcg cgcgaacgcg tcaacggcat tgcggcgcgt ttcggtgggg
     3601 cggcgttgcg cgtctgtccc gcgccggaaa cggcttcggg gtgaggagcg gcggttccga
     3661 gaatcttgaa ccacagaggg acacaaagcg cacagagaaa agcctgctaa aggtctttct
     3721 ccgtgccctc cgtgatctct gtgtttcagc ctttgggtct ttaagcgccg cggatgcgtc
     3781 ccagcagcgc ggtggtcgag cgctgatgtt cgaaggggat ggcgtgcacc gtgccgcccc
     3841 agccgcgcac ttcctcggcg ccgacgatcg cgtccggctg ccagtcgccg cctttcacca
     3901 gcacctcggg ccggcaggcg agtatggccg ccagcggcgt gtcctcctcg aaccaggtga
     3961 cgagatcgac gcattcgagc gccgccatga ccgccatgcg atccgccagc gtgttgatcg
     4021 ggcggtcctc gcctttgccc agccgcttca ccgaggcgtc gctgttcagt gcgacgacca
     4081 gactcgcgcc gagcgcgcgc gcctgcgcca gataggtgac gtgaccgcga tggaggatgt
     4141 cgaatacgcc gttggtgaag accagcggtc gtgcaagggc agcggcacgc gccggcaatt
     4201 cgtcgggcgt gcacagtttg gtttcgaagg acggacgcgg atagtcgttc gactgcatcg
     4261 acctggcctc cgcgtcccgc atatcaaacc gctgcaggtg cagcgcccaa ccccttacga
     4321 tagcgattca gttcctgcac cgtctcgatt gtctgctcga acagaatcga acagttgcgc
     4381 aggatggccg tcaccacctt gccttcccac accttgtcga atttgatctg ctcgtccagc
     4441 cagtgctcca gccagtccgg gtcgggcagc ttggactgca ccgtatcctg cggatagagc
     4501 tccttgttga cgtgcagatt ggtcgggtgc agcggcttgc tgctgcgccc ggaactcgcc
     4561 atcagcatgc cgatcttggc aaaggcgcga cgcgcctcgt cgccgcagcg ttcgatggcg
     4621 cgcttcatgt actggcggta aacgccgccg tgacgcgcct cgtcgtgcga aaggatctcg
     4681 tagatcttct tgatcaccgg ttcggtatgc cactccgaag cacggcggta ccagtgattc
     4741 aggcggatct cgccgcagaa gtgcagcatc agggtttcca gcggcggcgc ggggtcgaac
     4801 tggaagcgca cggcgtgcag ttcctcttcc gtgggcgcga gttcggggcg gaaacgccgc
     4861 agatactcca tcagcaccag cgagtgcttc tgttcctcga agaaccagac cgacatgaat
     4921 gcggagaaat cgctgtcatg ggcattgtcg cggaggaaca tttccgtggc gggaagcgcc
     4981 gcccactcgg tgatggcgtt catcttgatg gtgtgcgcct gatcgtcgct caatttgctg
     5041 ccatcgaatt gatcccacgg cacgtccttg cccatgtccc agcggacgcg ttcgagggat
     5101 ttgaaaatct ctggataaag catgttctcg agaataaaag gaccaaggaa gtgcgcatgc
     5161 gcgcacggac aaacgctgca gtgtagcagt tttggcccgc ttagcccgcc aggcggtcgg
     5221 ccggttcccc gtgtgcctca gtgagtggaa aatgcgccga cactctggct ttggcgccaa
     5281 ctggcgacgg cctcggggat catgtcgaaa cggggtaggg cgacgacatc ggccggcgcg
     5341 cgccgcatga tggcggggtt cgaactgccg atccagacct gagccgactc gggcagttgc
     5401 tcgcgcaacg cgcccagtcc ttccagcgcc tgcgtgatcg gatacgttgc gctgaaataa
     5461 agggcgacga tatcggcctg ctgcgcgcgc gcggcgagca tcatgtccac caccggcgcc
     5521 tgcgcgccga gcgacaggca actggcgcct tcgctcgcca gcaggcattc catcatcgtc
     5581 aatcccagcg tgcgcgattc gttgggaaag gtgcccagca gtacgcgcgg cgtgcggttc
     5641 tggagcggaa tcgcccctaa gccgccctgc agcgtgctga gcagcgcttc ggtgtaaagc
     5701 cgctcttcat agagcgcgag ttgaccgcgc atccaggcgt cgctcatctg ctgatgcaga
     5761 tcgacgacgg tctgcagcac gaaatcgcgc agcccctcgc tggccaggcg atcgcgcagt
     5821 tgctgcttga tttcggcgac gtcctgcgtg ttgaatgcgg caaaaggctg ggtcggtgtt
     5881 gctgcgcccg acgcgatcgc gcgcgccggc tgcggatcga ccagttgcag caattcggtc
     5941 acgctgcggg agaccagctt gcccggccgg tggccgcggt cgatgagccg cttcagcagc
     6001 cgcagctttt ccacctgctc ctgcggataa acccgttccc cgaacttgtc gcgctccggc
     6061 tgcggaaaac cgtagcgctt ctcccagacg cgcaacgtat ctttggacag gccggtatcg
     6121 cgctccaccg ccccaatgga taagcgccag cgctgggcgt aattcccgtc ttgcatggtc
     6181 gttccgagtt tgttcatggc gagcctcctg ccctgtctag cagacggcgt tcctgtcgtg
     6241 cacagcggac acttttgact tgcgttgcgg ctgcggtttg cgcggcgcgc tacaaaaggc
     6301 gccggggcgc ttgccaggga gtctcgacgg cggttaggat tccaccttaa ggaatgcgcc
     6361 taagcagttg ctataaaaac acatataagc ggcgcggata aaggggagag caggtgacgg
     6421 tgaggccctg gtattggacg cgcgatatgc gccgacgtac gcacccgtac ggtggcgctg
     6481 ccgataacaa catgcgcccg ggaaagagga gccagcgtgc aatcgttacc gcagccgcct
     6541 aagcagacaa accgacgttt tccctcgtcg gacttcgcgt caaccggtcg cgcgccgtgc
     6601 gcccgccgct gcgcaggcaa gcgagcgata cgatgctgac gcgtcgcgac atacggcccg
     6661 atgcgaagac ggaagcgcgc acgacgcgcg ccgatccggc cttcaattac ttccagcaca
     6721 ccatagccga actggagcag cgcctggcgc acctgagcga ggcgcaacgc gcgctgcgca
     6781 tcagcgagga gcgctatcgc gcactgtacg acagcaatcc ggtgatgagt ttcaccgccg
     6841 acaccgacgg tactgtcctt tccgtcaatg cctatggtgc gcagcgcctc ggctatgaac
     6901 ccgaggaatt gatcggccgc agcgtgttcg atctggcctt cgcggccgat cgccgggccg
     6961 tgcgacaggc gtttgccgat tgcctgcgcg aggccggcca tgtccatagc ggggagttcc
     7021 gcaagcggcg ccgcgacggc caggtggtat gggtgcgcga aaccctacgt gcggtgcagg
     7081 agcccggcgg tgcgctggtg gtgttgctgg tttgcgaaga ggccggcgtc tcgcacgcgg
     7141 tcgagtcccg cacgcgcgag gcggcggtgc gactcgaatt ggccctcgcc ggcgccaatg
     7201 ccgccatgtt caactgggac atcgacagcg gtgcagtgca tttgagcgag cgctggcggg
     7261 aaatgctcgg cgatttgccg cgcgaaactg tcaccaccat ccgggcgctc agcgatgtgg
     7321 tgcatgcgga cgacatccgc caattccgcg aacgcgtgct ggatgcggta aagggcatca
     7381 cgcccgcgct cgaatacgaa ttccgcgccc gtgcgcgcaa cggcgattgg cgctggatca
     7441 tcacccgcgg caaggtcgtc gagcgcggtg cggacggccg ggccgtacgc atgatcggca
     7501 ccaacaccga cgttaccgcg ctgcggcagg cggaggaggc gctgcgtcgc gcggaggcgc
     7561 gcctgcgcag tcttaccgag ttgtcctccg actggtactg ggagcaggac gagacgctgc
     7621 gttacacgca tgtgaccggc gggatgctcg cgcagaccgg tttcgaccca aggcaggata
     7681 tcggccggac gtgctgggaa ctcgaaggcg tcgaaggcgg cgagccgcac tggcccgacc
     7741 atcgccgcgc actggcgcgc cgccagccgt tccacgactt cgtctataaa cggcggaatc
     7801 tggccggcga gttgcgttat cacagcctga gcggccagcc ggtctacacc gcagacggcc
     7861 ggttcagcgg ctactgcggc atcggccgcg atgtcaccga ccgcttcaag gccgagcagc
     7921 gcatccatcg gctggcgcat tacgactcgc tctcgggact gcccaatcgc gcgctcttcc
     7981 atcagcgtct ggagcaggcc ttggcgcggg cccgccgatt cggccgcacg ctggccgtat
     8041 tgagcctgga tctggaccgc ttcaagagca tcaacgacag cttctgccac agcgccggcg
     8101 atcaggtgct gtgcgaagtg gccgaacgtc tgacgcgcgc taccggcgcg acggacacgg
     8161 tcgcgcgcct cagcggcgac gaattcgtgg tgctgctgga aagctgcgaa agcgccgcgg
     8221 aggtgtccgc cttcgcccgt tcagtgctgg cgacgctcgg ccggccgtat acggtcgccg
     8281 gccaggaatg ccatctcacc gcgagcatgg gcatcagcct ttatccgcag gacggtggcg
     8341 acggtctggc gttgttgcgc gatgccgaca ccgcattgaa ccgcgcgaaa gagaagggca
     8401 agaactgctt ccagttctat tcggcgcgca tgaacgcgca ggcctacgaa cggctggcgc
     8461 tggagtcgaa gctgcgccgc gcgctggagc acgaggaatt cgtgctgcac tttcagccca
     8521 ccgtggagtt gggcagcgga cgcatcgtca gcgtcgaggc gctggcacgc tggccgcaag
     8581 ccgacggcag catgatcgag ccggcgacgt tcattcccgt ggccgaagac agcggcctca
     8641 tcgaagtgct gggtcagtgg gtgctgcgca aagcctgcgt gcaggcgaag gcatggcgtg
     8701 cggccggact gccgcccatc cgcgtggcgg tcaacctgtc ggcgcggcag tttctgcagg
     8761 aagatctgct cgcccaggtg gcggcggtga tcgccgagac gggcatcgag cccgcgctgc
     8821 tggaattcga aatcaccgaa agcctgatgt tcgccaatcc gcaacatgct gccggcctgc
     8881 tgcatgcgct caagctcatg ggcgtgcgac tcgcgctcga cgatttcggc accggctatt
     8941 ccagcctgtc ctatctcaag cgctttccct tcgacagcgt gaagatcgac cgctcgttcg
     9001 tgcgcgacct gccgcaggac aacgacgacg cggccattac gcgcgcagtc atcgcgatgt
     9061 cgcacagcct gcgcatgacg gttaccgccg agggtgtgga aagcgccgcg caattggcct
     9121 ttctgcgcga atgcggttgc gacgcggcgc agggcaacca cttcagtccg gcgctgccgg
     9181 ccgaccgctt cgcggcattg ttcgatcgcg atctgcgcac ccagccgatc cggctggtcc
     9241 gctgaatgcg gaaaagaaaa gggccgacga gtgtcggccc gtggagcggg attacttgga
     9301 cggctgcaac tgcacgtatt cgaacagccg caccactctt tgcacgccgc tggtcgagcg
     9361 cgcgatttcg gtcgcttcct cggcctcctc gacggtcacc aggcccatga gatagacgac
     9421 gcgggcttcg gtcaccacct tgacgtgcag cgggttgaac ttctgctggt tgagcatgcg
     9481 cgtcttcacc tggctggtga tgtaggtgtc gcgcgagcgg ctgtccagcg aggagggcag
     9541 accgatctgc acctcgttgt acaccttgcg cacgtcgtgc acccgcgagg cgacatcgtc
     9601 gatcaccgcc ttgccttcgg gcgtggtgac ttcgccggtc agcagtactt ggcggttgta
     9661 gctggtgacg ttgatgtgca cctgattgcc cagccgcttt tcgatttccc gactcatggt
     9721 ccattcgatg cgctgatcgg tggcaacgat gtccggcgga cggcggtcgg caatgagcgc
     9781 tgcgccgccg gcggtgccgc ccacgaccag cgggaagcaa ccggcggtca gcaccgaggc
     9841 gcaaaaaatc agtacggcgg ctgcgagacg tcggttcatg cgttcactcc gttcctaaca
     9901 gtagacagtc gatgccgtcg cacaaacagt gcagcgtcag caaatgcacc tcctggatgc
     9961 gggcggtgcg gttgtgcggc acgcagatgt gcacatcgtt cggcgtcagc catttggcca
    10021 tttccccgcc ggacttgccc gtcaacgcca ccacgcgcat ttcgcgttcg tgcgccgcat
    10081 gaatcgcggc cacgacgttg ggcgaattgc cgctggtgga gatggcgagc agcacgtcgt
    10141 tcggctgccc cagcgcaagc acctgcttgg aaaagacctg gttgtagtcg taatcgttcg
    10201 cgatcgacgt cagcgtcgag gtgtcggtgg tcagcgcaat gccggccagt ccggggcgtt
    10261 cgcgttcgaa gcgattcagc aactcggcgg agaaatgctg cgcgtcggcc gccgagccgc
    10321 cgttgccaca ggcaagaatc ttgccgttgg cgagtaaaca tttgaccatc gcatcgaccg
    10381 cttcggcgat cggcgcggtg agcgcatcca ccgcgtcgag cttggtctgc gcgctgtcgt
    10441 tgaactgctg gcggatgcgt gcgggaaggt ccatcgaagc ctaaaccgta gaaagaagcc
    10501 ggcattttac tctacgggca ttgcgcgcga tcgggagcgg cgcaccgtgc gcgtcaaaca
    10561 ttgcggcggt caggcgccga atgcatcgcg cagccattcg atacgcgctt cgtcgagcgc
    10621 gtcgagcacg acgacgtcga agcggcaggg ttgctctccc cgatccatca ggtagtggcg
    10681 cgccgcgctg agcaggcggc gctgcttggc gccgtgcacg ctcgccgccg caccgccgaa
    10741 ggcggccgaa cggcgcaacc gcacctccac gaacaccagc gtgtcgccgt cctgcgcgat
    10801 gagatcgatt tcaccggtgc ggcagcggta gttgcgcgca agaatgcgca ggccgcggcg
    10861 ttccagccag gcggcggcca ggtgctcggc agcctgaccg tcgctcgctt tcactccagt
    10921 acgatctgct cgccggacag gaagatggcg agccatgggc gccgctctac gcttccgttc
    10981 ggctgcacgc tgatgtgtcc ggtcagtccg tcgagccgga tgtcctgccg gccctgggcc
    11041 agttgcgtgg cgatgcgata ggcatcgacg ccgagcgcat agaagcgtag cgcgccgccg
    11101 gagagatcgg cggggcgggg gaagttcacg aagctctcgt gctccggcgc cagcagccag
    11161 ggcatatcca cgaaccgcac gccgttcaga tccaccatgc cgaccggatc gatcggaaag
    11221 gagttgatct gcgaggtcgc atagaccggc acctgcgagg gcagataggg ccggatcagt
    11281 cgcgcgtcct ggtattgcgc ggcaaagtag acggcgtcga ggtttaactc cgttagcatg
    11341 gtgcgcatgc cggtgaggtc gctggcgggc gacgcgtcca cggaagcgac cgcttcgccg
    11401 ccgagcttgc gccattcgtc gagaaaggcc tcgcgcgagc gatgcgacag cggcgtgggc
    11461 gtgctcaccg cgccgatgcg ccgcatgccg tcgcggaagg cacgttgcgc aagcatgcgc
    11521 gcttcgtcct ccaccgacag accgaaggca taaagccccg gcggtaaggg ttgctcgtta
    11581 tcaggttgat tgagcgtgag cgtgggcacc atcaccacgc cgctggcggc gatggctgag
    11641 acaccggagc gtgtcatggg gccgacgatc accgccggca cttgctggag cacttcgaga
    11701 taggcctgat agatgccttc ggggctcgcg tcggtggcgc gcacttcgat cggcagcggc
    11761 gcggggtcca gcagggcggc cgcttcgacg ccgcgcacga agggataggc ggcgcggcgg
    11821 aagtcgggcg aatccagcgg caggagcagc gtcatgatgg gcgcgcgctg cgacatatcc
    11881 ggcagcggcg gcacgacacc ctgctcaggc tccgtgcccg gtgtgattgg cgtttcgggc
    11941 aacggctcgc cggggagtac gggctgaccg tcgggcgtga tctgcaattc gggttgtgcg
    12001 gccggcgcgg tcggttgcgg tgcggttgcg ggcggctggg tggcacagga caatgccagg
    12061 ctcgccgcca gcaatgcggc gcacagggaa cgaatcaggg aatgaggggg cacgcggaaa
    12121 gactcatgaa ggaagagcgc gtcgcattat atgtggtggc cacgccgatc ggtaacctgg
    12181 gcgacattac gcagcgcgcg ctcgacacct tgcgcgatgc ggatgtcatt tacgccgagg
    12241 atacgcgcac cagcgctgcg ctgctcgcac atcacggcat ccgtacgccg ctgaaagccc
    12301 tgcacgagca caacgaagcg cgtgccacac ccgaggtatt gcgcgcgctg gacgcgggtc
    12361 gggcggttgc gctcatcagc gatgcgggca cgccggcaat ttccgatccg ggcgccatgg
    12421 tggtggccgc ggcgcgcgaa gcggggcacc gcgtcgtgcc cattcccggc ccgaatgcgg
    12481 ccatcgccgc cttgtcggct tcggggatga cggggccgtt cctgttcgtc ggttttctgc
    12541 cgcccaaggc cggcgcacgg cgcaagctgc tcgaatcctg gcgcagcgcg ccgcatgcgc
    12601 tcgtgttcta cgaggcgccg catcgcgtgc tcgaatgcgt cgatgacctg ctacaggttt
    12661 tcggacccgc ccgcacgctt acaatcgcgc gggagctgac caagctgtac gagacgattc
    12721 acgtctgtac gctcggcgag gcgccggcct ggttcgctga agacgccaac cgtttgcgcg
    12781 gcgaattcgt gctgatcgtc tccggcgcac cggcggacga agacggagcg ctggccgacg
    12841 gcgagcgcgt gctgaaactg ctgctggagg aactgccgct caaacgtgcg gcgcggctcg
    12901 cggccgatat caccggcgcg cggaagaacg aattgtacga gcgcgggctc gcgtacaagg
    12961 acgacgacaa ggacgagtaa tgcaaagcat caccctcacc cggccggacg actggcacct
    13021 gcatttgcgc gacggcgccg cacttgcgag cgtactgccg cacagcgcgc ggcaattcgc
    13081 ccgcgccatc atcatgccga acctcaggcc gccggtgacg acggtggaac tggccgccgc
    13141 ctatcgcgaa cgcatcctcg ccgcactgcc ggccggcatg cgcttcgagc cgctgatgac
    13201 gctctatctc accgacaaca ccacggcggc ggaaatcgcc acggcgaagg cgagcggttt
    13261 cgtgcatgcg ctcaaatact atccggccgg cgccaccacc aactcggact ccggcgtgac
    13321 gtcgctcgac cgcgcttcgg cggcgctggc cgccatggaa gaacacgacg taccgctgct
    13381 ggtgcacggc gaagtcacca cgccgggcgt ggacgtgttc gatcgcgaga aagtgttcat
    13441 cgacaccgtg atgagcgaca tcgtgcggcg cttccccggg ctgcgcgtcg tattcgaaca
    13501 catcaccacc ggcgacgccg cggcgttcgt gcgcgacagc gggccgaaca tcggcgccac
    13561 catcaccgcg catcatctgc tctacaaccg caatgccatt ttccagggcg gcatccggcc
    13621 gcattactac tgcctgccgg tgctcaagcg cgaacagcat cgcgaagcct tgatcgccgc
    13681 ggcgacgggc gagagcaaca agttcttcct cggcaccgac agcgcgccgc atccgcgtca
    13741 cgacaaggaa acggcctgcg gttgtgccgg ctgcttcacc gcgcatgcgg cgatcgagtt
    13801 gtatgcagag gcgttcgaac aggccggcgc gctcgacagg ctggaagcct tcgccagctt
    13861 caacggtgcg gacttctacg gtctgccgcg caacgaagag cgcatcacgc tggtacgcgc
    13921 tccctggcag gtgccgcgca gcgtgattta cgccgacgcc gaactcacgc cgctgcgcgc
    13981 cggcgaatcg ctggcctggc gcatgcagta ggccatgacg gccgtcacgc cgcgctggaa
    14041 ccgggcggcc ctgctcgcct cgccgttgct gcataccgtg cggccgttca tcgaagcgct
//
LOCUS       DATGOU010000050         4236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3232583, whole genome shotgun sequence.
ACCESSION   DATGOU010000050 DATGOU010000000
VERSION     DATGOU010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3232583"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VKZ48_02705"
     CDS             <1..443
                     /locus_tag="VKZ48_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HLU76797.1"
                     /translation="RFLRLWANGADEADDARLREYVLAMRPHLAFMPHGNDSNRTHRR
                     VYRSFDAIARAEGLALWAFLNRDAKTDSMRLDLYCDFDEEEARWKASLLRLHRSQQAR
                     NLNTRNMGFDDRVLAMNRESARLVGAALPYAECFELQRYSGAHG"
     gene            556..1713
                     /locus_tag="VKZ48_02710"
     CDS             556..1713
                     /locus_tag="VKZ48_02710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLU76798.1"
                     /translation="MRCLNRNDILDYVRMRRAFFDAASATNPFVGSDWLERFIEHVAQ
                     DDWRFIAPEHRDETSSLMLLYSRADDPQRWFSVTNHYASLYTPLIGATQQHAAVARVL
                     AERITRETPDCISINCAPLDGDAAGTVALERAFAEQGWHTKRYSCFGNWYLPCAGLSF
                     ERYLHGRDAMLRTTVARKTRRFELGEDGARLVLLSTPAEVEAGMAAYERLRTRSWNRA
                     EPYPDFVHAWARTCAQRGWLRLGLAFVHDTPIAAQFWFTRHRRAYIFKLAYDESYARF
                     SAPTVLSAHMIRHSLEVDRVEEIDYLTGDDVYKRAWMPRRRERIGLMLCNPRTMRGQL
                     LSTHQLASEARQRWLRSTRPAPMSALPRDKRRLAARQAGGHNADVSASHHA"
     gene            1716..2042
                     /locus_tag="VKZ48_02715"
     CDS             1716..2042
                     /locus_tag="VKZ48_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007877175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76799.1"
                     /translation="MSKPYRHSVYVIELDERVLNHRRFREANPGRDILKPCLYVGATG
                     LSVEQRFANHKRGYKANRYVQLYGIRLRPEFYACFNPMPYHAALTMEVELAEDLRDQG
                     YAVWQG"
     gene            2178..2498
                     /locus_tag="VKZ48_02720"
     CDS             2178..2498
                     /locus_tag="VKZ48_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLU76800.1"
                     /translation="MKQSLIFRAALAAVAMGLASPAFADLELAKKYSCVACHQIDKKS
                     IGPAYKDVAAKYKGDAAAPARLADHVKNGSVGVWGQMPMPANSQVPDEDVKKLVAWIL
                     AGAK"
     gene            complement(2554..3051)
                     /locus_tag="VKZ48_02725"
     CDS             complement(2554..3051)
                     /locus_tag="VKZ48_02725"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HLU76801.1"
                     /translation="MKPHSSGRAWLAALAILTVALSGCAGYPLREPIEVYVADIESLP
                     GEGLEVRMMVKLRIQNPNDETIVFDGVALNMDLQGRPFATGVADVRGEVPRYGETLVA
                     VPVSLSVFGIARRAVDAVTDVYQRGRVNYELSGKLARSEERPLYFESDGQFSLPEELY
                     GRFPR"
     gene            complement(3111..>4236)
                     /locus_tag="VKZ48_02730"
     CDS             complement(3111..>4236)
                     /locus_tag="VKZ48_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLU76802.1"
                     /translation="NLVEQPNRRIDADVSLRTPITPSWALVTGVSWNRSMLDRETSDA
                     ANWREPDTEGAMLNSERGRSTNIALFVQSEHYLPYDLTAYVGGRYDYFETTGHVRDVA
                     SGFDERYGKRDFEQFSPKLALVWEPRHWLALRTSYGEGFRPPALLDLYGRIVAPNPGA
                     GPGVMRVTDPAPDLEPERVRAWEVGADMAFARGKRLSVTLYRQRLEDLIYRHNLSDTQ
                     ARTENAGEAEINGIEASMRWPTPLPNLSAVASYTHQFKYEITRNDAVPESVGKVLTDV
                     PRTAWSAGLEYERGAWSGLLLVRHVGHVYGSGDDVNANKVEGVFGSYDEYTVVAARLG
                     WRINRHLALSVSIDNLTDRDYFVYNRQPGRTFYTELSYRF"
BASE COUNT          758 a         1405 c         1318 g          755 t
ORIGIN      
        1 tgcgcttcct gcgtctgtgg gcaaacggcg ctgacgaagc cgacgacgcc cgtctgcgcg
       61 aatacgtgct cgcgatgcgc ccgcacctgg cgttcatgcc gcacggcaac gacagcaatc
      121 gcacccaccg gcgcgtctat cgaagcttcg atgccatcgc acgcgccgaa ggcctggcgc
      181 tgtgggcctt cctgaaccgg gacgcgaaga ccgattcgat gcgtctcgac ctctattgcg
      241 acttcgacga ggaggaagcg cgatggaagg cttcgctgct gcgactgcat cgctcgcagc
      301 aggcgcgcaa cctcaatacg cgcaacatgg gtttcgacga ccgcgtgctg gccatgaacc
      361 gcgaaagcgc ccgcctcgtc ggcgccgcac tgccttacgc ggaatgcttc gaattgcagc
      421 gctacagcgg cgcgcacggt taatttttgt cacaagcggt ccgggcgggt ccgctcgagc
      481 ggccccgctc ttatcctcgt cccctcagtg tcgccaaata gagacggtgc gtccgatgca
      541 ccgagggaga ccgcgatgcg ctgcctgaac aggaacgaca ttctcgacta cgtccgcatg
      601 cgtcgggcct tcttcgacgc agcaagcgcg accaacccct ttgtcggcag cgactggctg
      661 gaacgattca tcgagcatgt ggcgcaggac gactggcgct tcatcgcgcc cgaacaccgc
      721 gacgagacga gcagcctcat gctgctgtac agccgtgccg acgatccgca gcgctggttc
      781 tcggtcacca atcattacgc gtcgctttac acgccgctga tcggcgcgac gcagcagcac
      841 gccgcagtcg cgcgcgttct ggccgagcgc atcacccggg aaacgccgga ttgcatctcc
      901 atcaattgcg cgccgctgga cggcgatgcg gcgggcaccg tcgcgctgga acgtgccttc
      961 gccgaacagg gttggcacac caagcgctac agctgcttcg gcaactggta tctgccctgc
     1021 gcgggcctgt ccttcgagcg ctatctgcac gggcgcgacg cgatgctgcg cacgacggtg
     1081 gcgcgcaaga cgcgccgctt cgaactgggc gaggacggcg cccgcctcgt tctgctttcc
     1141 acgccggccg aggtggaagc gggcatggcg gcctatgaac ggctgcgcac gcggagctgg
     1201 aaccgcgccg agccctatcc cgatttcgtt cacgcctggg cgcgtacctg cgcgcaacgg
     1261 ggctggctgc gtctgggttt ggcttttgta cacgacacgc ccatcgccgc gcagttctgg
     1321 ttcacacgcc atcgccgcgc ctatatcttc aagctcgcct acgacgaaag ctatgcacgc
     1381 ttttccgcgc cgaccgtgtt gagcgcacac atgatccggc acagcctcga agtggaccgc
     1441 gtcgaggaga tcgactacct caccggtgac gacgtctaca aacgggcctg gatgccgcgc
     1501 cgccgcgagc ggatcggact catgctgtgc aacccccgca cgatgcgcgg gcaactgctc
     1561 tccacccatc agttggccag cgaggcgcgc cagcgctggc tgcgctccac ccgccccgca
     1621 ccgatgtcgg cgctgccgcg cgacaaacga cggctggccg cgcggcaggc cggcgggcac
     1681 aatgcggacg tctccgcatc gcaccatgcc tgaacttgag caaaccgtac cgccacagcg
     1741 tttatgtgat tgagctggac gagcgcgtcc tcaaccatcg caggttccgc gaggcgaatc
     1801 ccggccgcga cattctcaaa ccctgtcttt atgtgggcgc gacggggttg tcggtcgaac
     1861 aacgcttcgc caatcacaag cgcggttaca aggccaaccg ctatgtgcag ttgtacggca
     1921 tccgcctgcg gcccgagttt tatgcctgtt tcaaccccat gccttatcat gcggcgttga
     1981 ctatggaagt cgaattggcg gaagatttgc gcgaccaagg ctatgccgtc tggcaaggct
     2041 gaggccgttc aagcgcggtt cggctctgtg cttgttctta caacccttcg gacttcatgg
     2101 aattccgcca tgcgcatacc cgtgcgatat cacgcgtgtc catcgatgtc gtcgtcgttt
     2161 actgattgga gtaatgaatg aagcaatccc tcatcttccg cgctgctctt gccgccgtgg
     2221 caatgggcct ggcctctccc gccttcgcgg atcttgaact ggcgaagaag tactcctgtg
     2281 tcgcctgcca ccagatcgac aagaaatcga tcggcccggc ctacaaggat gtagccgcga
     2341 agtacaaagg cgatgccgcc gcgcccgcgc gtctggccga ccacgtgaaa aacggcagcg
     2401 tcggcgtatg ggggcagatg cccatgccgg ccaattcgca agtgccggac gaggacgtga
     2461 agaagctggt tgcctggatt ctggccggcg cgaagtaacg cgcaagccat ggcaacaacg
     2521 gggcggcggt gagccgccct tttttattcc cgcttagcgt ggaaaacggc cgtacagctc
     2581 ctcgggcaac gagaactggc cgtcggattc gaaatagagc ggccgctctt ccgagcgcgc
     2641 gagcttgccg ctgagttcgt agttcacgcg gccacgctga tagacatcgg tgacggcgtc
     2701 cactgcgcga cgcgctatgc cgaacaccga aagactgacc ggcaccgcca ccagcgtctc
     2761 gccgtagcgc ggcacttcgc cgcgcacatc ggccacaccg gtcgcgaagg gtcgcccctg
     2821 cagatccatg ttgagcgcca cgccatcgaa cacgatcgtc tcgtcgttgg gattctggat
     2881 gcgcagcttg accatcatgc gcacttcaag cccctccccc ggcaacgact cgatatcggc
     2941 gacgtacacc tcgatcggtt cgcgcagcgg atagcccgcg cacccgctca gcgcgaccgt
     3001 caggatcgca agcgccgcaa gccacgctct accactcgaa tgaggcttca tggtccggtc
     3061 cgggcgatgt ccggcatgga cgcatgcgag cggcgtgcca tgccggcacg tcagaaccgg
     3121 tagctcagtt ccgtatagaa agtgcgtccc ggctgccggt tgtaaacgaa gtagtcgcgg
     3181 tcggtgaggt tgtcgatgga gacgctcagc gccagatggc ggttgatgcg ccagcctagc
     3241 cgcgcggcga cgacggtgta ttcgtcgtag gaaccgaaca cgccttcgac cttgttcgca
     3301 ttgacgtcgt caccggagcc gtagacgtgc ccgacgtggc gcaccagcag caggccggac
     3361 catgcgccgc gctcgtattc cagcccggcc gaccaggcgg tgcgcggcac atcggtcagc
     3421 accttgccga ccgactcggg cacggcgtcg ttcctggtga tttcgtattt gaactgatgc
     3481 gtgtacgagg cgaccgcgct caggttcggc aaaggcgtag gccagcgcat gctcgcttcg
     3541 atgccgttga tttccgcctc gccggcgttt tccgtacgcg cctgcgtatc cgagaggttg
     3601 tgtcggtaga tcaggtcttc gagccgttgg cggtagagcg tgaccgacag acgcttgccg
     3661 cgcgcgaaag ccatgtcggc gccgacctcc cacgcgcgca cgcgttccgg ctccagatcg
     3721 ggcgcgggat cggtgacgcg catgacgccc ggacccgcgc cgggattggg cgcaacgatg
     3781 cggccgtaca gatccagcaa tgcaggcggc cgaaagccct cgccgtaaga cgtgcgcaag
     3841 gccagccaat gcctcggctc ccacaccagc gcgagcttcg gactgaattg ttcgaaatcg
     3901 cgcttgccgt agcgctcgtc gaacccggag gcgacgtcgc gcacatggcc cgtcgtttcg
     3961 aagtagtcgt aacggccgcc gacataagcg gtgaggtcgt acggcaggta atgctcgctt
     4021 tgcacgaaca gcgcgatgtt ggtcgaccgg ccgcgctcgg aattcagcat cgcgccctcc
     4081 gtatccggtt cgcgccagtt ggcagcgtcg gaggtttcgc ggtccagcat gctgcggttc
     4141 cacgacacgc cggtcaccag cgcccatgaa ggcgtaatcg gcgtacgcag cgatacgtcg
     4201 gcatcgatgc gccggttggg ctgctcgacc agattg
//
LOCUS       DATGOU010000051        11383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_2787548, whole genome shotgun sequence.
ACCESSION   DATGOU010000051 DATGOU010000000
VERSION     DATGOU010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11383
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2787548"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /gene="tolR"
                     /locus_tag="VKZ48_02735"
     CDS             complement(<1..406)
                     /gene="tolR"
                     /locus_tag="VKZ48_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012401830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HLU76803.1"
                     /translation="MRQRKLMNQINVVPYIDVMLVLLVIFMVTAPMLNPTLVELPSVG
                     KSAAQVPETPLEVIVKADATMVLRERTQGKPTDRNVDLQGLVEAIRLKQAQSPDQAVV
                     ISADKAVRYEAVLKVMDELQRHGVARVGLMVKS"
     gene            complement(476..1147)
                     /gene="tolQ"
                     /locus_tag="VKZ48_02740"
                     /pseudo
     CDS             complement(476..1147)
                     /gene="tolQ"
                     /locus_tag="VKZ48_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584943.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
     gene            complement(1172..1594)
                     /gene="ybgC"
                     /locus_tag="VKZ48_02745"
     CDS             complement(1172..1594)
                     /gene="ybgC"
                     /locus_tag="VKZ48_02745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HLU76804.1"
                     /translation="MSEGASRPFAWPVRVYFHDTDAGGIVFHGNYLHFMEAARTEFLQ
                     SLGYNVAELQRAEDGVLFVVYALELKYRKPARLHDSLQITVAVQHVGGARLIFEQRVV
                     RDGETLVSAQVSIATVHPRTMKPVPVPERLRAQVQTNS"
     gene            complement(1587..2630)
                     /gene="ruvB"
                     /locus_tag="VKZ48_02750"
     CDS             complement(1587..2630)
                     /gene="ruvB"
                     /locus_tag="VKZ48_02750"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HLU76805.1"
                     /translation="MAIETDRLISPEPAGSQEEALERALRPKQLDDYVGQEKIRHQLQ
                     IFIEAARSRHEALDHVLLFGPPGLGKTTLAHIIAREMGVGLRQTSGPVLERPGDLAAL
                     LTNLEPHDVLFIDEIHRLSPVVEEILYPALEDYQIDIMIGEGPAARSVKLDLPPFTLV
                     GATTRAGMLTNPLRDRFGIVARLEFYTPEEVQRIVSRSAALLNVEVDSSGSLEVARRS
                     RGTPRIANRLLRRVRDYAQVKAEGRITREVADAALSMLDVDTLGLDVMDRKLLLAVIE
                     KFGGGPVGVDNLAAAIGEERDTIEDVLEPYLIQQGYLQRTPRGRTATLSAYRHFGLAA
                     PGSGAAGELFDNE"
     gene            complement(2721..3302)
                     /gene="ruvA"
                     /locus_tag="VKZ48_02755"
     CDS             complement(2721..3302)
                     /gene="ruvA"
                     /locus_tag="VKZ48_02755"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HLU76806.1"
                     /translation="MIGRIHGTLLEKNPPQILVDCQGVGYEVEVPMSTFYNLPPNGER
                     VTLLTHMVVREDAQLLFGFGAEAERRLFRLLIKISGVGARTALAVLSGMSVDDLAEAV
                     SLQETARLTKVPGIGKKTAERLLLELKDKLGVELGAAGAVNRPASGSSDVLHALLALG
                     YNDREARAAVKQLPEDVPVSDGIKQALKSLSKA"
     gene            complement(3308..3724)
                     /locus_tag="VKZ48_02760"
     CDS             complement(3308..3724)
                     /locus_tag="VKZ48_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76807.1"
                     /translation="MVAWVTLLKAGLPYVAEVVVDRLPAFTKRGEGANNTAELTAQQI
                     AELQQAARHNAESIKAVAEQLQQTVKAIETGAQEQALVIEALRKQVADMAEQRDNVTK
                     ERDVLRRRIEAARWVSLIAFGTAFLALSLSIFSNIA"
     gene            complement(3737..4288)
                     /gene="ruvC"
                     /locus_tag="VKZ48_02765"
     CDS             complement(3737..4288)
                     /gene="ruvC"
                     /locus_tag="VKZ48_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HLU76808.1"
                     /translation="MRILGIDPGLRITGFGVIEKNGTKLGYVTSGCIRSPDAGGLPER
                     LKVILESLGEVIALHRPTQVAIEKVFVNVNPQSTLLLGQARGTAICAAVMAQLPVCEY
                     TALQVKQAVVGSGHAKKEQVQEMVKRLLSLPGLPGADAADALACAICHAHGGQGLGAL
                     ATSGYRVRGGRLVEGRARRRTTV"
     gene            complement(4369..5094)
                     /locus_tag="VKZ48_02770"
     CDS             complement(4369..5094)
                     /locus_tag="VKZ48_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020748022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLU76809.1"
                     /translation="MAGHSKWANIKHKKAAADAKKGKVFTRLIKEITVAARMGGGDPN
                     MNPRLRLAMDKAQEANMPKENVERAIKRGTGELEGVSYEEIRYEGYGIGGAAVMVDCL
                     TDNRTRTVADVRHAFTKHGGNLGTDGSVAFMFKHCGLLVLAPGADEDKVMEVALEAGA
                     EDIVTNDDGSIEVITAPADFEAVKAALEAADLKPEYAEVTQRAENETELTGDDAEKMQ
                     RLLDALEAIDDVQNVYTTAVMPE"
     gene            5204..6706
                     /locus_tag="VKZ48_02775"
     CDS             5204..6706
                     /locus_tag="VKZ48_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HLU76810.1"
                     /translation="MPVPLPIAQSDATALALLPALANRHGLITGATGTGKTVTLQVLA
                     ENFSRIGVPVFLSDVKGDLSGLAVAGQPTPKLKQRLDTLGLPEPVWQDCPVAFWDVYG
                     EQGHPVRTTVSELGPLLLGRMLDLNDTQQGVLTLTFKVADDHGLLLLDLKDLRAMLQY
                     VGDNAKTIKTAYGNVSAASIGAIQRGLLELEHQDAERFFGEPALDITDFMQTDRGRGV
                     INILAADRLLNAPKLYSTFLLWMLSELFEQLPEVGDADKPKLVFFFDEAHLLFNDAPK
                     ALVDKIEQVVRLIRSKGVGVYFVTQNPLDIPDAVLGQLGNRVQHALRAFTPRDQKAVK
                     AAAQTMRPNPAFDAEQAITELGVGEALVSLLDEKGRPGVTQRAYILPPGSQIGPISEA
                     ERRAIIRRSVLYGHYEKAVDRESAYETLKGRAEASAAAADSPSVETPQSEGGWLDRLG
                     EVLGGSTGPRGGRREGAIEAAAKSAARSIGSQIGRELTRGVLGSIFGKRR"
     gene            6703..7617
                     /locus_tag="VKZ48_02780"
     CDS             6703..7617
                     /locus_tag="VKZ48_02780"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLU76811.1"
                     /translation="MSATPASPGAYRTVGFLLALLGVLSFSIRPVLVKVAYTYARDPV
                     TLLALRMVFSLPFFAFAAWWLQRNKPAAPIETRDKWAIVGLGVIGYYLASFFDYLSLQ
                     YITAGLSRLIHFTYPTIVVLLGMLFLRKAPRVAEVLALMLTYVGLAVMLLSTAAGQDE
                     FLALGAAMSFGSAVSYAVYLFAGTQVIQRVGSMRFAAYALMVSSFCCILQFVLLRPLD
                     ALFIPAEVYWLSLIIATVSTVLPIFSIAEALKRLGANSVAIMGALGPVSAMVLGYLGL
                     DEIMTGRQIFGSALVLLGVVLVTLKPRR"
     gene            complement(7626..7865)
                     /locus_tag="VKZ48_02785"
     CDS             complement(7626..7865)
                     /locus_tag="VKZ48_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76812.1"
                     /translation="MSLNQALAFLENGDWESAHPIVQNESGPTAAWAHGIVHILEGDY
                     PNARYWYGRAGRAYPGPDRVREELAELKQALAAGA"
     gene            7954..9429
                     /locus_tag="VKZ48_02790"
     CDS             7954..9429
                     /locus_tag="VKZ48_02790"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HLU76813.1"
                     /translation="MNAPDTAFPYPLRDASLLRTRCYIDGEWRGAADDRRLSVRNPAT
                     GELIATVPNAGAVETRAAIEAANAAWPAWRATTAKQRSDILRKWHALMLQHLEDLAVI
                     MTAEQGKPLIEARGEINYAAAFMEWFAEEAKRVYGETIPATQGNRRIVVLRQPVGVCT
                     AITPWNFPSAMITRKVAPALAAGCTMVVKPASATPLSALALAELAERAGVPKGVFSVV
                     TGEAKPIGEEMTTNPLVRKFSFTGSTETGKLLLKQCAGTVKRTSMELGGNAPFIVFDD
                     ADLDAAADGALASKYRNAGQTCVCANRILVQSGVYDAFAEKLAERVRKMKIGNGMEPG
                     VNIGPLIDEATVAKAEDHIADALSKGARVVHGGKRHALGGLFFEPTILADVDTSMKVT
                     REETFAPVAPLFRFDDEAEAVRLANDTEFGLAAYFYTRDLSRAWRVSEALEYGMVGVN
                     AGLISSEVAPFGGVKESGLGREGSHLGIEEYLEVKYVGINI"
     gene            9465..10331
                     /locus_tag="VKZ48_02795"
     CDS             9465..10331
                     /locus_tag="VKZ48_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013954124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HLU76814.1"
                     /translation="MKKALGVYSAPQGHWVGDGFPVRSLFSYNTHGKRLSPFLLLDYA
                     GPAEFAPAEKPRGVGTHPHRGFETVTIVYKGEVSHRDSTGKGGTIGPGDVQWMTAGAG
                     ILHEEFHSERFTRTGGPMEMVQLWVNLPAEHKMTRPGYQAIVAQDIPQIALPDDAGEV
                     RVIAGAYNDRKGPASTFTPMNVWDLRLRAGGIAQIDQPDGWNTAIVALHGTMLVNGEK
                     ILREAQMVVLDREGTGVGIEANNDAVALLLAGEPIDEPIVGYGPFVMNTHAEIEQAMR
                     DFQSGRFGEIGA"
     gene            complement(10335..11162)
                     /gene="murI"
                     /locus_tag="VKZ48_02800"
     CDS             complement(10335..11162)
                     /gene="murI"
                     /locus_tag="VKZ48_02800"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HLU76815.1"
                     /translation="MQRALIIDSGVGGLSVAYAARVALPELNIAYVADNAWFPYGKRS
                     EAALIERLVHLVEHSLRISPGDAVVVACNTASTVVLDALRSQFNVPIVGVVPPIKTAG
                     EVSQSRVVGLLATEATVTRPYVRRLKEEFAADCRIIPLGLSRLAEIAEEKVRGIPVDR
                     AELRELLKPFFGRDALHVDTVVLGCTHYPLLLDDFKAVSPPDVQWLDSSPAIARQLAR
                     VLSDVPPHNDGPLKDAVYFTAPVEAVDALTAYLASLRLPAPQLLPESAEVALRLARG"
BASE COUNT         1986 a         3762 c         3575 g         2060 t
ORIGIN      
        1 ccgatttcac catgaggccg actcgcgcga cgccgtgccg ctgcaattcg tccatcacct
       61 tgaggacggc ttcatagcgc acggccttgt cggccgaaat caccaccgcc tgatcgggcg
      121 attgggcctg tttgagcctg atcgcctcga ccagcccctg caggtcgacg ttgcggtccg
      181 tcggtttgcc ctgggtgcgt tcgcgcagca ccatcgtcgc atccgccttc acgatcactt
      241 ccagcggcgt ctcgggcact tgcgccgccg acttgcccac gctcggcaac tcgaccagcg
      301 tcgggttcag catcggcgcc gtcaccatga agatgacgag cagcaccagc atcacgtcga
      361 tatacggcac gacgttgatc tggttcatga gcttgcgttg tcgcatgacc tacttcgcca
      421 acggttgcgg cgcgcgctgt gcggcgtgcg gaagttgcga gtgcgacatc gggttgtgcg
      481 cctgccgctg caggatgttg gaaaactctt ccatgaagct ttcgaaatgg atcgaaagcc
      541 ggtcgatgtc gtgcgagtag cggttgtacg ccaccaccgc gggaatagcg gcgaacaggc
      601 cgatcgccgt ggccaccagc gcttcggcga tgcccggcgc cacggcggcc agcgtggccg
      661 agctcacatt cgccaggccg cggaacgcat gcatgatgcc ccacaccgtg ccgagcaggc
      721 cgacataagg gctcaccgag ccgaccgaag cgagaaaagc aagatgcgat tcgagatagt
      781 cgatttcgcg ctggtaggtt gcgcgcatcg cacgacgcgc gctgtccacc agatcgctcg
      841 tatcgacgtc ctgtgctttc agcttcacgt agtcgcgaaa gcccgcttcg aagatgcgct
      901 ccagtccacc ggtgcgatgc cgggcattgg cggcgctttg ataaagcgcg ttgagatcgg
      961 ggcggctcca gaaggtacgc tcgaaatcgg ccgtctggcg gcgcgccgtg cggatgacga
     1021 acatcttgtt gaagatgtag taccaggaga tgaccgacac gccgagcagc agcgccatga
     1081 tgccctgcac tgcccaactg gcgttgagga tcagttcgag gatcgaaaga tcttgggtaa
     1141 cgttcatgaa tgattcggcg cgatgccgtc gttagctgtt cgtttgaacc tgggcacgca
     1201 gtcgctcggg cacaggcacc ggcttcatgg tgcgcggatg gacggtggca atgctgacct
     1261 gcgcgctgac gagcgtttcg ccatcgcgca cgacgcgctg ctcgaagatc agtcgggcgc
     1321 cgccgacatg ctgcaccgcc acggtgatct gcagggaatc gtgcagtcgc gccggcttgc
     1381 ggtatttcaa ttccagggca tagacgacga atagcacacc gtcctctgcg cgctgcagtt
     1441 cggcgacgtt ataccccagg gactgcagga actcggtgcg cgcggcttcc atgaaatgca
     1501 aataattgcc gtggaagacg atgccgcccg catcggtgtc gtgaaaataa acccggaccg
     1561 gccatgcaaa cggccgactt gctccttcac tcattgtcga acagctctcc cgccgcaccg
     1621 cttccgggag cagccagacc aaaatgacga tatgcgctca gcgtcgccgt gcgtccgcgc
     1681 ggcgtgcgct gcaaataccc ctgctggatc agataaggct ccaacacgtc ttcgatggtg
     1741 tcgcgctctt caccgattgc ggcggcgagg ttgtcgacgc cgaccggccc accgccgaat
     1801 ttttcgatca cggccagtag caatttgcgg tccatcacat ccaggccaag cgtgtcgacg
     1861 tcgagcatcg acagcgcggc atccgcgact tcgcgcgtga tgcgtccttc cgccttcacc
     1921 tgcgcgtagt cgcgcacgcg gcgcagcagg cggttggcga tacgcggtgt cccgcgcgaa
     1981 cgacgcgcca cttccagcga gcccgacgaa tcgacttcga cgttcagcag tgcggccgag
     2041 cgcgaaacga ttctttgcac ttcttcgggc gtgtagaact ccagccgcgc gacgatgccg
     2101 aagcgatcgc gcaacggatt ggtcagcatg cccgcgcgcg tggtcgcacc gaccagggtg
     2161 aaaggcggca gatcgagctt caccgagcgc gcagccggac cctcgccgat catgatgtcg
     2221 atctgataat cctcgagcgc gggatagagg atctcctcca ccaccggcga cagacgatgg
     2281 atctcatcga tgaacagcac gtcgtggggc tcgagattgg tcaagagtgc cgccagatcg
     2341 cccggccgct ccagcaccgg cccggaggtc tgccgcagcc cgacgcccat ctcacgcgcg
     2401 atgatgtgcg cgagcgtggt cttgcccagg ccgggtgggc cgaacagcaa cacatggtcg
     2461 agcgcctcgt gccgcgagcg cgcggcttcg atgaagatct gcagctgatg gcggatcttc
     2521 tcctgcccga catagtcgtc gagttgcttc ggccgcaggg cgcgctcgag cgcctcctcc
     2581 tgcgatcctg cgggttccgg cgaaatgagc cggtcggttt caatggccat gggcgcgaat
     2641 tatggcattg atccgcggcg cactcgaaag cgggacacgc cggcggacgg tttgcgcggc
     2701 ggcgcgccgc gaatccggcg tcatgccttc gataaggatt tgagcgcctg cttgatgccg
     2761 tcggacaccg gcacgtcctc gggcaactgt tttaccgccg cgcgggcttc gcggtcgttg
     2821 tagccgagcg ccagcagcgc gtgcagcacg tcgctcgatc cggatgccgg gcgattgact
     2881 gcaccggcgg cgcccagttc gacgcccagc ttgtctttca attccaacag caggcgttcg
     2941 gccgtcttct tgccgatgcc cggcaccttg gtcagccgag ccgtttcctg caggctgacc
     3001 gcctcggcca gatcgtccac cgacatcccc gacagcacgg ccagcgcggt gcgcgcaccc
     3061 acgccgctga tcttgatcag cagacggaac aggcggcgct cggcctcggc gccgaagccg
     3121 aacagcagtt gcgcgtcctc gcgcaccacc atgtgcgtaa gcagcgtcac ccgttcgccg
     3181 ttcggcggca ggttgtagaa ggtgctcatc ggcacctcga cctcgtagcc gacgccctgg
     3241 cagtcgacca atatctgcgg cggattcttc tccagcagcg tcccgtgtat ccgtccgatc
     3301 atggcgctca ggcgatgttg ctgaaaatgg acagcgacag cgcgaggaag gccgtaccga
     3361 aggcgatcag gctgacccag cgcgccgcct cgatgcgccg ccgcagcacg tcgcgctctt
     3421 tcgtcacgtt gtcgcgctgc tcggccatgt cggccacctg cttgcgcagc gcctcgatca
     3481 ccagtgcctg ttcctgcgcg ccggtttcga tggctttcac ggtctgctgc aactgctccg
     3541 ccaccgcctt gatggattcg gcattgtgcc gtgcggcttg ctgcaattcc gcgatctgct
     3601 gcgcagtgag ttcggccgtg ttgttggcac cttcgccccg cttggtgaag gcgggcaacc
     3661 gatcgaccac gacttcggcc acatagggca ggccggcctt caatagagtg acccatgcga
     3721 ccatttcgct cgctccttaa acggtggtgc gccgacgcgc ccggccttcg accagccgcc
     3781 cgccgcgtac gcgatagccg ctcgtcgcca acgcgcccaa cccttgtccg ccgtgcgcat
     3841 gacagatggc gcaggcgagc gcatcggcgg cgtccgcgcc cggcagaccg ggcagcgaca
     3901 gcagccgctt gaccatctcc tgcacctgct cttttttggc gtgaccgctg ccgaccaccg
     3961 cctgtttcac ctgcagcgcc gtgtattcgc acaccggcaa ctgcgccatg accgcggcgc
     4021 agatcgccgt accgcgggcc tggccgagca gcagggtcga ttgggggttc acgttgacga
     4081 acactttttc gatcgccacc tgcgtcggcc gatggagcgc aatgacttcg ccgagcgatt
     4141 cgagaatgac tttcagccgt tcgggcagtc cgccggcgtc cggcgagcgg atgcagccgc
     4201 tggtgacgta gccgagcttc gtgccgttct tctcgatgac gccaaaaccg gtgatccgca
     4261 atccgggatc gataccgaga atgcggattt cagccacggc gcctctccat ggatacggcg
     4321 gtgatccgca agcggggatc gatgccgagt atgcggatgg cgctcaaatc actccggcat
     4381 cactgccgtg gtgtagacgt tctgtacgtc gtcgatcgct tccagcgcgt ccagcaggcg
     4441 ctgcattttc tcggcgtcgt cgccggtcag ttcggtttcg ttctccgccc tttgcgtcac
     4501 ttccgcatac tccggcttga gatcggccgc ctccagcgcc gccttcacgg cctcgaagtc
     4561 tgcgggcgcc gtgatgacct cgatgctgcc gtcgtcgttg gtgacgatat cttcggcgcc
     4621 ggcctccagg gccacttcca tcaccttgtc ctcgtccgcg cccggcgcga ggaccagcag
     4681 gccgcagtgc ttgaacatga acgctaccga gccgtcggtg cccagattgc cgccgtgctt
     4741 ggtgaaagcg tggcgcacat cggccaccgt ccgcgtgcgg ttgtcggtca ggcaatcgac
     4801 catcaccgcc gcgccgccga tgccatagcc ttcatagcgg atttcctcgt agctcacgcc
     4861 ctccagttcg ccggtgccgc gcttgatggc gcgttcgacg ttctctttcg gcatgttggc
     4921 ttcctgcgcc ttgtccattg ccaggcgcag acgcgggttc atgttcggat cgccgccgcc
     4981 catacgggcg gccacggtaa tttccttgat cagccgggtg aagaccttgc ctttcttggc
     5041 gtcagccgcg gctttcttgt gtttgatgtt ggcccattta ctgtgtccag ccataacgcg
     5101 ctcactgcct aaaaatgtgg tcggaaaccg aagttcgccg aataatattc tggtccctgc
     5161 ggaaagcagc caattttaca cttcacccca gaggagtgcc gtgatgcccg ttccgttgcc
     5221 tatcgcccaa tccgacgcaa cggcgctcgc cctgttgccc gcgcttgcga atcgccacgg
     5281 cctcatcacc ggcgccaccg gcacgggcaa gaccgtcacg ctgcaggtgc tggcggagaa
     5341 tttctcccgc atcggcgtgc cggtgttcct ctccgacgtc aaaggcgatc tttccggcct
     5401 cgccgtcgcc ggccagccga cgcccaagct caagcagcgg ctcgacaccc tgggcctgcc
     5461 ggaaccggtc tggcaagact gtcccgtcgc attctgggac gtgtacggcg agcagggcca
     5521 cccggtgcgc acgacggtgt ccgaactcgg cccgctgctg ctgggacgca tgctcgatct
     5581 gaacgacacc cagcagggcg tgctcaccct caccttcaaa gtggccgacg atcacggcct
     5641 gctgctgctc gatctgaagg acctgcgcgc catgctgcag tacgtgggcg acaacgccaa
     5701 gacgatcaaa accgcctacg gcaacgtctc ggccgcttcg atcggcgcca ttcagcgcgg
     5761 cctgctggaa ctggaacacc aggatgcgga acgctttttc ggcgaaccgg cgctggatat
     5821 cacagatttc atgcagaccg accggggacg cggcgtcatc aacatcctgg ccgccgaccg
     5881 gctgctgaac gcgcccaagc tctattcgac cttcctgttg tggatgctgt ccgagttgtt
     5941 cgagcagttg cccgaggtcg gcgatgcgga caagcccaag ctggtgttct ttttcgacga
     6001 agcgcatctg ctgttcaacg acgcgcccaa ggcgctggtc gacaagatcg agcaggtcgt
     6061 acgactcatt cgttccaagg gcgtgggcgt ttacttcgtc acgcagaatc cgctcgatat
     6121 tccggacgcg gtgctcggtc agctcggcaa tcgcgtgcag cacgctttac gcgccttcac
     6181 cccgcgcgat cagaaggcgg tgaaagcggc cgcgcagacc atgcgtccca atccggcctt
     6241 cgatgcggaa caggcgatta ccgaactggg cgtcggcgaa gcgctcgttt ccctgctcga
     6301 cgagaaaggc cggccgggtg tgacccaacg cgcctatatc ctgccgcccg gctcgcagat
     6361 cggcccgatc agcgaagcgg agcgtcgcgc catcattcgc cggtcggtgc tgtacggtca
     6421 ctacgaaaag gcggtggatc gcgagtcggc ctacgagacc ctcaaaggcc gcgccgaagc
     6481 gtcggccgcg gccgcagact cgccgtcggt cgaaacgccg caaagcgaag gcggctggct
     6541 cgaccggctg ggcgaagtgc tgggcggcag caccggtccg cgcggcgggc gacgcgaggg
     6601 cgcgatcgaa gccgcggcca aaagcgcggc gcgctccatc ggttcgcaaa tcggccgcga
     6661 attgacccgc ggcgtgctcg gctccatttt cggcaaacgg cgttgagcgc aacgcccgct
     6721 tccccgggcg cctatcgcac cgtcggcttt ctgctggcgc tgctgggcgt gctgtctttt
     6781 tccatccgcc cggtgctcgt gaaggtcgcc tacacctacg cgcgcgatcc ggtcacgctg
     6841 ttggcgctgc gcatggtgtt ctcgctgccc ttcttcgcct tcgccgcctg gtggctgcaa
     6901 cgcaacaagc ccgccgcacc gatcgagacg cgcgacaagt gggcgatcgt cggcctgggc
     6961 gtgatcggct actacctcgc gagcttcttc gactatctga gtctgcagta catcaccgcg
     7021 gggctgtcgc gcctgatcca cttcacctat ccgacgatcg tcgtgctgct cggcatgctc
     7081 tttctgcgca aggcgccgcg cgtcgcggag gttctggcgc tgatgctgac ctatgtcggc
     7141 ctggcggtga tgctgctcag cacggcggcg gggcaggacg agtttctcgc cctcggggcg
     7201 gcgatgtcct ttggcagcgc ggtgagctat gcggtctatc tgttcgccgg cacgcaggtg
     7261 atccagcgcg tgggctcgat gcgcttcgcc gcctacgcgc tgatggtatc gagcttttgc
     7321 tgcatcctgc agttcgtgct gctgcgccct ctggacgcac tgttcattcc ggccgaggtg
     7381 tactggctgt cgctgatcat cgcgacggtg tcgaccgtgc tgccgatctt ctccattgcc
     7441 gaagcgctca agcgcctggg cgcgaacagc gtggccatca tgggcgcgct cgggccggtg
     7501 agcgcgatgg tgctcggcta tctggggttg gacgagatca tgacgggccg gcagatcttc
     7561 ggctcggcgc tggtcctgct cggtgtggtg ctggtgacgc tgaaaccccg ccgttgacgc
     7621 aggcgctatg cgccggcggc aagcgcctgc ttgagttcgg ccagttcctc gcgcacgcgg
     7681 tcggggccgg gataggcacg gcccgcgcgg ccgtaccagt agcgcgcgtt gggataatcg
     7741 ccttccagga tatgaacgat gccatgcgcc caggccgcgg tcgggccgct ttcgttttgg
     7801 acaatggggt gcgccgattc ccagtcgccg ttctcgagga acgcgagcgc ttggttgaga
     7861 ctcatatcgc caatcatgca agtgaagctg agacgccccc aagtatagtt ttgtgcgtcc
     7921 tcatcgccca cccgttgcag agagccttat tttatgaatg cacccgatac cgccttcccc
     7981 tatccgctgc gcgatgcatc actattgcgt acgcgctgct atatcgacgg cgaatggcgc
     8041 ggcgccgccg acgatcgccg cctgtccgtg cgcaaccccg ccaccggcga gttgatcgcc
     8101 accgttccga acgccggcgc cgtcgaaacg cgtgcggcca tcgaagccgc gaacgcggcc
     8161 tggcccgcct ggcgcgcgac caccgccaag caacgctcgg acattctgcg caagtggcac
     8221 gcgttgatgc ttcaacacct ggaagatctg gccgtcatca tgacggcgga gcaaggcaag
     8281 ccgctcatcg aagcgcgggg cgaaatcaac tatgccgcgg cattcatgga atggttcgcc
     8341 gaagaagcga agcgcgtgta cggcgagacc atcccggcta cacagggcaa ccgccgcatc
     8401 gtcgtgctgc gtcagccggt cggtgtgtgc acagcgatta cgccatggaa ttttccgtcg
     8461 gcgatgatca cgcgcaaggt cgcgccggcg cttgccgccg gctgcaccat ggtggtcaag
     8521 cccgccagcg ccacgccgct ttcggcgctg gcgttggccg aactcgccga acgcgcgggc
     8581 gtgcccaaag gggtgttcag tgtcgtcacg ggcgaagcca aaccgatcgg cgaggaaatg
     8641 acgacgaacc cgctggtgcg caaattcagc ttcaccgggt ccaccgagac cgggaagcta
     8701 ctgctcaaac agtgcgccgg cacggtgaaa agaacgtcga tggaactggg cggcaacgcg
     8761 cccttcatcg tattcgacga cgccgatctc gacgcggccg ccgatggcgc gctcgcgtcg
     8821 aagtatcgca acgccggcca gacctgcgtc tgcgccaacc gcatccttgt gcagtcgggc
     8881 gtgtacgacg ccttcgcaga gaagctcgcc gagcgcgtgc gcaaaatgaa gatcggcaac
     8941 ggcatggaac cgggcgtgaa catcggcccg ctcatcgacg aggcaacagt cgccaaagcc
     9001 gaagaccaca tcgccgatgc cctgtccaag ggcgcgcgcg tcgtgcacgg cggcaagcgg
     9061 catgcgctcg gcggcctgtt cttcgagccc accatactcg cggacgtgga caccagcatg
     9121 aaggtcacgc gcgaggagac cttcgcgcct gtcgcaccgc tgttccgctt cgacgacgaa
     9181 gccgaagccg tccgcctggc gaacgatacc gagttcggcc tcgccgccta cttctacacg
     9241 cgcgatctca gccgcgcgtg gcgcgtgtcc gaagcgctcg aatacggcat ggtcggcgtc
     9301 aacgccggat tgatctccag cgaagtcgcg ccgttcggcg gcgtcaagga atccggcctc
     9361 ggccgcgaag gctcgcacct gggcatcgag gaatatctgg aagtgaagta cgtcggcatc
     9421 aatatctgac gatggcgcgg ccggcgcatc cgcaggagca cggcatgaaa aaggctttgg
     9481 gtgtctacag cgccccgcaa gggcactggg tgggcgacgg ctttcccgtg cgctcgctgt
     9541 tcagctacaa cacgcacggc aagcggctca gccccttcct gctgctcgac tacgcgggtc
     9601 ctgccgagtt tgccccggca gagaagccgc gcggcgtggg cacgcatccg catcgcggct
     9661 tcgagaccgt gaccatcgtc tataagggcg aggtgtcgca tcgcgactcg accggcaaag
     9721 gcggcaccat cggtcccggc gatgtgcaat ggatgacggc gggcgccggc atcctgcacg
     9781 aggaattcca ctccgagcgc ttcacccgta ccggcggccc gatggaaatg gtgcagttgt
     9841 gggtcaacct gcccgccgag cacaagatga cgcggcccgg ctatcaggcg atcgtcgcac
     9901 aggacattcc gcagatcgcg ctgccggacg acgccggtga ggtgcgcgtg atcgccggtg
     9961 catacaacga ccgcaaaggc cctgccagca ccttcacgcc gatgaacgtc tgggatttgc
    10021 gtctgcgtgc gggcggcatc gcgcagatcg atcaaccgga tggctggaac accgctatcg
    10081 tggcgctgca cggcaccatg ctggtcaatg gcgaaaaaat cctgcgcgaa gcgcagatgg
    10141 tggtgctcga tcgcgaaggc accggcgtcg gcatcgaagc gaacaacgat gccgtcgcgc
    10201 tgctgctggc cggcgaaccg atcgacgaac cgatcgtggg ctacggtccc ttcgtgatga
    10261 acacgcacgc agaaatcgag caggcgatgc gggatttcca gtcgggccgt ttcggcgaga
    10321 tcggcgcctg agcgtcaccc ccgcgcgagc cgcagggcga cttcggcact ctccggcaac
    10381 agctgcggcg caggcagtcg cagagacgca agataggccg tcagggcatc aacggcctcg
    10441 accggcgcag tgaagtacac ggcgtctttt aacggcccat cattatgcgg cggcacgtcg
    10501 ctcaaaaccc gggcgagctg ccgcgcaatg gcgggcgacg aatcgagcca ctgcacatcg
    10561 ggcggagaga ccgctttgaa gtcatcgagc agcagcggat agtgggtgca gccgagcacc
    10621 accgtatcca catgcagcgc gtcccgaccg aagaacggct tcagcaactc gcgcagttcg
    10681 gcccgatcca ccgggatgcc gcgcaccttc tcctccgcga tctccgcaag cctcgacaat
    10741 ccgagcggaa tgatcctgca gtccgctgcg aactcctcct tgagccgccg cacatacggc
    10801 cgcgtcaccg ttgcttccgt agcgagcaac ccgactacgc gcgactgcga cacctcaccc
    10861 gccgtcttga tcggcggcac cacgccgacg atgggcacat tgaattgcga acgcaacgca
    10921 tcgagcacga cggtgctggc cgtgttgcat gcaaccacta cggcatcacc gggggaaatg
    10981 cgcaggctat gttcgacgag atggaccagc cgctcgatca gcgcggcttc gctgcgcttg
    11041 ccatagggaa accaggcgtt gtcggcaacg taggcgatgt tgagctcggg aagcgcaacg
    11101 cgagccgcat acgcaaccga aaggccgccc acgccggaat cgatgatgag tgcgcgctgc
    11161 atgatattga cgaatggaga gcgacgaata taccgcatcg ccatgccaca gacagcatgg
    11221 gcttacagac tcacgcacgc agacgcctca cccggttgga gatcaagtcg tcagagcatt
    11281 gcgagctcgt cggcgtgtcg tgtcgctcgc ctacggcgtg acgttgtcga ctgctgttgt
    11341 aagcgcggac ggtccgtgcg caccggaacg atcaggtctg tct
//
LOCUS       DATGOU010000052         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_5895074, whole genome shotgun sequence.
ACCESSION   DATGOU010000052 DATGOU010000000
VERSION     DATGOU010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5895074"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VKZ48_02805"
     CDS             complement(<1..382)
                     /locus_tag="VKZ48_02805"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HLU76816.1"
                     /translation="MLRTAYNGLLHLILPLLPLRLWWRGRKEPGYLAEVPQRFGRYGS
                     PATTRQPLIWLHAVSLGETRAAAPLVRALRTRYPAHRLLITQMTATGRAAAQESYGDF
                     AEIAWLPYDFPWAMRRFLAHYRPAL"
     gene            complement(382..1344)
                     /gene="waaC"
                     /locus_tag="VKZ48_02810"
     CDS             complement(382..1344)
                     /gene="waaC"
                     /locus_tag="VKZ48_02810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HLU76817.1"
                     /translation="MTAALLIRVSSLGDVVQTFPAVTEMSRHAAVSELDWAVEEAYVP
                     LVRMHPGVTHARPFALRRWRTAPFKRQTWRELSAFRAALRERPYQAVIDAQGLLKSLA
                     IAKSAHGPLYGFGPETVREPFVSRFYDHSFEFSPDQHRIWQYKGLMAKTFGYEMDDVI
                     DYGLTKPVRPGWLGESGYVVLLHSTARAEKLWPEVKWIEVGRRLASRGMVCVLPWGNA
                     EEHARAERLAKLIPDAQVAPQLRVDEAGALLAHAHAVIGVDTGLMHLAVAFEVPVVGI
                     YVATRPLHHGPLGRGPTAFRGGMGEAPTTAEVLDALGEVAPGLA"
     gene            complement(1468..>2113)
                     /locus_tag="VKZ48_02815"
     CDS             complement(1468..>2113)
                     /locus_tag="VKZ48_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLU76818.1"
                     /translation="DLWFAAITKLAAAGIHGGAGQVDWQVVKRLWSGSLPLALLVVVL
                     VSIGTKIQKVDWLTQAVGVVILITAVGLLFAPKLARAAHGRRLSQPQKFKAVQPVLTV
                     GAGAVLGICVALTSVGAGALGSVMLLYLYPLRMTPHRLVATDIVHAIPLAIVAGLGYL
                     VAGMVDWRMLASLVVGSIPAVVIGSLLARKFSARWLRVALSAVLMAAAVKTLTA"
BASE COUNT          443 a          718 c          643 g          309 t
ORIGIN      
        1 cgagtgccgg ccggtagtgc gcgagaaaac ggcgcatcgc ccagggaaag tcgtacggca
       61 gccaggcaat ctcggcaaag tcgccgtagc tttcctgcgc cgctgcccga ccggtggccg
      121 tcatttgcgt aatcagtaaa cggtgggccg gatagcgcgt tcgcaaagcg cgaacgagcg
      181 gtgcggccgc acgcgtttca ccgagcgaaa cggcatgcag ccagatcaag ggttgtcgcg
      241 tcgtcgccgg ggaaccgtaa cgaccaaagc gctgcggcac ctcggccaga tagcccggct
      301 ccttccgccc ccgccaccac aatcgcagcg gcaacagcgg caggatcaaa tgcagcaatc
      361 cgttgtaagc ggtgcgcagc atcaagcgag accgggcgcg acttcgccca gggcatccaa
      421 cacctcggcc gtagtcggcg cctcgcccat gccgccccgg aaggccgtcg gcccgcgacc
      481 cagcggacca tggtgcagcg ggcgcgtcgc cacataaatg cccaccaccg ggacttcgaa
      541 cgccaccgcc agatgcatca ggccggtgtc cacaccgatg accgcatgcg catgcgccag
      601 caaggcgccc gcctcgtcga cgcgcaactg cggcgcgacc tgcgcgtccg ggatcagctt
      661 tgcgagacgt tcggcgcgcg cgtgctcttc cgcattgccc cacggcaaca cacagaccat
      721 tcctcgcgac gccagccggc gtccgacctc gatccacttc acctccggcc aaagcttctc
      781 ggcgcgcgcg gtcgaatgca gcaagaccac gtagccggat tcgcccagcc acccgggccg
      841 tacgggcttc gtcaggccgt agtcgatgac gtcgtccatc tcatagccga aggtcttagc
      901 catcaggccc ttgtactgcc agatgcggtg ctgatccggg gaaaattcga agctgtgatc
      961 gtagaaccgc gagacgaagg gttcgcgtac ggtttcgggg ccgaagccgt agaggggacc
     1021 gtgcgccgat ttggcgatcg ccagactttt gagcagtcct tgcgcgtcga tgacggcctg
     1081 atagggtcgc tcacgcaacg ccgcgcgaaa cgccgacaac tcgcgccacg tctgccgttt
     1141 gaaaggcgcc gtacgccaac gccgcaatgc gaacggtctg gcatgcgtca ccccgggatg
     1201 catgcgcaca agcggcacat aggcctcctc cactgcccaa tcgagttcgg ataccgctgc
     1261 atgccggctc atttcagtga cggcagggaa tgtctgcacc acatcgccga gcgacgagac
     1321 gcgaatgagc aaagcagcgg tcatcgatat cgaatgggcg gatgaagcat gtccgggaca
     1381 cgcattatag gagtagcgac tcggctcacc gagcgccgtc agtcgattca gagggccgga
     1441 ggtgacggaa cctacatcgc aacgaattta agccgtcaag gtcttgactg cagcggccat
     1501 caagacggcg gacaaggcaa cccgcagcca tcgcgcggaa aatttccgcg cgagcaggct
     1561 cccgatcacg accgccggaa tcgaaccgac caccaggctg gcgagcatcc gccaatccac
     1621 catccccgca acgagatagc cgagcccggc gactatagca aggggaatcg cgtgcacgat
     1681 atcggtcgcg acgagccgat gcggcgtcat gcgcagcgga tagaggtaga gcagcatcac
     1741 actgccgagc gcgccggcgc ccaccgaagt cagcgcgaca cagatgccga gcacggcgcc
     1801 ggcgcccacc gtcaacaccg gctgaaccgc cttgaatttc tgcggctggg acaagcgcct
     1861 gccatgcgcc gcgcgcgcca gctttggtgc gaacaacaga ccgaccgcgg taatgagaat
     1921 gaccacgccg actgcctgcg tcagccagtc gaccttctgt atcttcgtac caatgctgac
     1981 cagcacgaca accaggagcg ccagcgggag gcttcccgac cacaggcgtt tgacgacctg
     2041 ccaatccact tgcccggcgc cgccgtgaat gccggctgcc gccagtttgg taatggctgc
     2101 aaaccaaaga tcc
//
LOCUS       DATGOU010000053         4471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_2343182, whole genome shotgun sequence.
ACCESSION   DATGOU010000053 DATGOU010000000
VERSION     DATGOU010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2343182"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /gene="dapB"
                     /locus_tag="VKZ48_02820"
     CDS             complement(<1..438)
                     /gene="dapB"
                     /locus_tag="VKZ48_02820"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HLU76819.1"
                     /translation="MKIAIAGAGGRMGRALIEAVLRTDDLELGGAFDVDGSAALGRDA
                     GEGIGRVSGVAVSADFSAAIAAGDCMIDFTRPEGTLRHLDACVQQGKAAVIGTTGFSA
                     ADKAAIEAAAQRIPVVFAPNMAVGVNVTFKLAEMAARILGDDYD"
     gene            complement(435..842)
                     /locus_tag="VKZ48_02825"
     CDS             complement(435..842)
                     /locus_tag="VKZ48_02825"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HLU76820.1"
                     /translation="MRFVALIPLVAALAVAGCSSNLNPLRPYRMEIQQGNHLSQENVA
                     KLKRGMTKDQVRFVLGTPLITDIFHADRWDYVYRYSPENSNTVEERRLTVIFDDERLV
                     RLEGDVVAAGGEASAQNEVEEPAPRSLFEEGRL"
     gene            998..1444
                     /gene="fur"
                     /locus_tag="VKZ48_02830"
     CDS             998..1444
                     /gene="fur"
                     /locus_tag="VKZ48_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006025247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HLU76821.1"
                     /translation="MTQAENLKTIGLKATLPRVKILELFERSEVRHLSAEDVYRLLLR
                     DNLDVGLATVYRVLTQFEQAGLLQRHHFESGKAVFELNDNDHHDHLVCMQCGHVEEFY
                     DAEIEKRQNRIAKERGFTIHEHSLYIYADCTKDPCPFREIGGKPTF"
     gene            1514..1984
                     /gene="sixA"
                     /locus_tag="VKZ48_02835"
     CDS             1514..1984
                     /gene="sixA"
                     /locus_tag="VKZ48_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004556863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HLU76822.1"
                     /translation="MHLILWRHAEAEDGFPDAQRPLTARGRKQAQRMATWLSQHLPQD
                     AQILVSPAVRTQQTAAALGRPFHTRKEISVGADAAQVLAAAGWPDGSGRTVVVVGHQP
                     TLGQVAARLLSGVEAYWSVKKGAVWWLSSRLRAGETQIVLRASISPDLLPDVRG"
     gene            complement(1981..3480)
                     /locus_tag="VKZ48_02840"
     CDS             complement(1981..3480)
                     /locus_tag="VKZ48_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017231892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HLU76823.1"
                     /translation="MHYESLAAVDLGSNSFHLQIGRVVDDQIYALDALREPVRLAAGL
                     DAEGNLDEPTQLRALATLSRFGERLRGFAPGAVRAVGTNALRVAGNASQFLERAHDAL
                     GFPVEVLYGREEARLIYLGVVHSLPSTRKRRLVMDIGGGSTEFIVGTGHKPALTESLS
                     MGCITYTLRFFPDGRIDKSSMKKAIIAAGEEAQAIVRKFVAVGWHEAVASSGTANTIA
                     AVLQAVGWGEGTITAAGLHRLRTHLVKTGTVEGLPTVRTDRQAILPGGVAVMCAAFEA
                     FGLEEMMVSEAALRQGVLYDLLGRVQHRDMRQSTVEQYMRRYQVDRDQAERVARLARQ
                     IYTSLADSPDEDELQLLDWAARLHEIGLSIARASYRKHSAYVIANADMPGFSKNEQAR
                     LARIVLAHCGKLPKPGLPAEDAERLLILSLRLAALFYRSRADLRLPLLQCRATERGFR
                     IDLPKHWLAAHPLTAAALEAEAQAWQGQGVNLDIRETGNSERPNPVAVP"
     gene            complement(3555..4256)
                     /gene="phoU"
                     /locus_tag="VKZ48_02845"
     CDS             complement(3555..4256)
                     /gene="phoU"
                     /locus_tag="VKZ48_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020069441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HLU76824.1"
                     /translation="MSISHTSKHFDAELERLRKRVLEMGGLVEQQIRNSVEALRTHDA
                     KLAAQVIADEERVNRMEREIDEECVQLLARRAPAALDLRFVLMAYKVITDLERVGDEA
                     KKIASMAERLQLSEHRSPGFPEIAIIAELVTDMLRRALDALARAESSDIPEVIRSDLE
                     VNERFHAILRALLTYMIEDPRTISASIDTLWIAKALERIGDHAKNISEYAIYMIKGED
                     VRHASLEELERAAKS"
BASE COUNT          796 a         1525 c         1387 g          763 t
ORIGIN      
        1 gtcgtagtcg tcgcccagga tgcgggccgc catttcggcg agcttgaacg tgacgttgac
       61 gccgacggcc atattcggcg cgaacaccac cggaatgcgt tgtgccgccg cttcgatggc
      121 ggccttgtct gcagcgctga agccggtggt gccgatgacc gccgccttgc cctgctggac
      181 gcaggcgtcc aaatgacgca gcgtgccttc cggccgggtg aaatcgatca tgcagtcgcc
      241 tgcggcgatc gccgcggaga aatcggcact caccgcgacg ccgctgacgc gcccgatccc
      301 ttcgccggca tcccgcccca atgcggcgct gccgtcgaca tcgaaggcac cgcccagctc
      361 gagatcgtcg gtgcgaagca ccgcctcgat cagcgcgcgg cccatgcggc cgcccgcgcc
      421 cgcgatggca atcttcacag ccgtccctct tcgaacaggc tgcgcggcgc cggctcctcc
      481 acctcgttct gtgccgaggc ttcaccgccg gcagccacca cgtcgccttc caggcgcact
      541 agccgctcat cgtcgaaaat cacggtgaga cgacgctctt cgaccgtgtt gctgttctcc
      601 gggctgtagc ggtacacata atcccagcga tcggcgtgga agatgtcggt gatcagcggc
      661 gtgccgagca cgaagcgcac ttgatccttg gtcatgccgc gcttcagttt ggcgacattt
      721 tcctgcgaca gatgattgcc ctgctggatc tccatccgat aaggacgcag gggattgagg
      781 ttgctgctgc agccggccac ggcgagcgca gccacgagcg gaatcagggc gacaaagcgc
      841 atagttatag aattctttcg cgtactcagg ggcgcgcaaa gccgatatca tagccgatga
      901 tcggccggct gccggccgcg ccatttcgat ttccggcgac agtcggagcg gtgtagtgta
      961 gcgccttcgc caggcgcccc ggacagaggg ccacatcatg acccaagcag agaatctcaa
     1021 gaccatcggc ctgaaggcca ccctgccccg ggtgaaaatc ctcgaattgt tcgagcgcag
     1081 cgaggtgcgg catctgtccg ccgaggacgt gtaccgcttg ctgctgcggg acaacctgga
     1141 cgtgggtttg gccaccgtct atcgggtcct gacccagttc gaacaggccg gccttctgca
     1201 gcgccatcac ttcgaatccg gcaaggcggt attcgaactc aacgacaacg accaccacga
     1261 tcacctcgtg tgcatgcaat gcggtcacgt cgaagagttc tacgacgccg aaatcgagaa
     1321 gcgccagaac cgcatcgcca aggaacgcgg cttcaccatc cacgagcact cgctctacat
     1381 ctacgcggac tgcaccaagg atccctgtcc cttccgcgaa atcggcggca aacccacgtt
     1441 ctgaccgccc tcgtcgttcg tccggtcgca acatgagtac ggcagcatga gcgacttcga
     1501 cgacgaaaac ggcatgcatc tcatcctctg gcgccacgcc gaagcggaag acggcttccc
     1561 ggacgcgcaa cgcccactca ccgcccgcgg ccgcaagcag gcacagcgta tggcgacatg
     1621 gctgtcgcag catctgccgc aggacgcgca gatcctggtg agcccggcgg tccgcacgca
     1681 acagacggcc gcggccttgg gccgcccctt tcacacccgc aaggaaatca gcgtcggcgc
     1741 cgatgccgca caggtgctcg ccgccgcggg ctggcccgac ggctccgggc ggacggtggt
     1801 ggtggtcggc caccagccca cgctcggaca ggtggcggca cgactgctga gcggcgtcga
     1861 agcgtactgg tcggtcaaaa agggcgcggt gtggtggctg agcagccgcc tgcgcgccgg
     1921 tgaaactcag atagtgctgc gcgcatcgat ctcgcccgat ctgcttccgg acgtccgcgg
     1981 ttaaggcacc gcaaccggat tggggcgttc gctgtttccc gtttcgcgta tatccaggtt
     2041 tacgccctgg ccctgccacg cctgcgcctc ggcctccagc gccgcagcgg tcagcggatg
     2101 cgccgccagc cagtgcttgg gcaggtcgat gcgaaaaccg cgttcggtgg cgcgacactg
     2161 cagcaacggc agcctcaagt cggcgcggct gcggtagaac aacgcagcga ggcgcagcga
     2221 cagaatgagc agccgctccg cgtcctcggc cggcaagccg ggtttgggca gcttgccgca
     2281 atgggcgagc acgatgcgcg cgagacgcgc ctgctcgttc ttggaaaagc ccggcatgtc
     2341 ggcattggcg atcacatagg ccgagtgctt gcggtagctg gcgcgcgcga tggacagacc
     2401 gatttcatgc agccgcgccg cccagtcgag cagttgcaac tcgtcctcgt ccggcgaatc
     2461 ggcgaggctg gtgtatatct gccgcgccaa ccgcgccacc cgctccgcct ggtcccggtc
     2521 cacctgataa cgtcgcatgt attgctcgac cgtgctttgc cgcatgtcgc gatgctgcac
     2581 ccggcccagc aggtcataca gcacaccctg acgcaaggcc gcctcggaca ccatcatctc
     2641 ttccagcccg aaggcttcga aggccgcgca catcaccgcc acgccacccg gcaggatcgc
     2701 ctggcgatcg gtgcggacgg tgggcaatcc ttcgacagtg ccggtcttga ccagatgcgt
     2761 gcgcagccga tgcagtcctg ccgcggtgat ggtgccctcg ccccagccga ccgcctgcag
     2821 gacggcagct atggtattcg cggtgcccga cgaggccacc gcctcgtgcc agccgaccgc
     2881 gacgaattta cgcacgatgg cctgcgcctc ttcgccggcg gcgatgatcg ccttcttcat
     2941 cgaactcttg tcgatgcggc cgtcgggaaa gaagcgcagc gtataagtga tgcagcccat
     3001 ggacaggctt tcggtcagtg cgggtttgtg gccggtgccg acgatgaact ccgtcgagcc
     3061 gccgccgata tccatcacca ggcggcgctt ccgcgtcgac ggcaggctgt gcaccacgcc
     3121 cagatagatc agtcgcgcct cttcgcggcc gtagagcact tcgacgggga agccgagcgc
     3181 gtcatgcgcc cgctccagaa actgcgatgc gttgcctgct acgcgcagcg cattggtgcc
     3241 gaccgcgcgt accgcaccgg gcgcaaagcc gcgcaggcgc tcgccgaagc gcgacagcgt
     3301 cgcgagcgcg cgcaactgcg tcggctcgtc cagattgcct tcggcatcca gcccggcggc
     3361 gaggcgcacg ggctcgcgca gcgcatcgag cgcatagatc tggtcgtcga ccacgcggcc
     3421 gatctgcagg tggaagctgt tcgaaccgag atcgacagcc gcaagggatt cgtagtgcac
     3481 gacgggaggc agcaaaaccg atgccgcacc ttagcaccgg cgtacgcgca cccgcaattg
     3541 tccggtcggc gcgatcagga cttcgctgcg cgctccagtt cctctagcga cgcgtgccgc
     3601 acgtcttcgc ccttgatcat gtagatcgcg tactcggaga tgttcttggc gtgatcgccg
     3661 atgcgttcca gcgccttggc gatccacagc gtgtcgatcg acgcggaaat ggtgcgcggg
     3721 tcctcgatca tgtaggtcag caatgcgcgc aggatggcgt ggaaacgctc gttgacctcc
     3781 aggtcgctgc ggatgacctc cgggatatcg ctcgattcgg cgcgcgccag cgcgtcgagc
     3841 gcacggcgca gcatgtcggt gacgagttcg gcgatgatcg cgatttcggg gaagccggga
     3901 ctgcggtgtt cgctcagttg cagccgctcc gccatggaag cgatcttctt cgcctcgtcg
     3961 cccacgcgct ccagatcggt gatcaccttg tacgccatca gcacgaagcg caggtccagc
     4021 gcagccggcg cgcggcgcgc gagcaactgg acgcattcct cgtcgatctc gcgctccata
     4081 cggttgacgc gctcctcgtc ggcgatcacc tgcgccgcca gcttcgcatc atgcgtgcgc
     4141 agcgcctcga ccgagttgcg gatctgctgc tcgacgagcc cacccatttc cagcacccgc
     4201 ttgcgcagac gctccagttc cgcatcgaaa tgcttggacg tatgcgatat cgacatgaca
     4261 gcagcctcca ataacgacac cggaactcta gcaccacttc atgacaatca tgttgcaaac
     4321 gcagttggcg gtgcgcagaa cgcggcatac cgcgcgccag gctgtaacat aaggtcaaca
     4381 ttggctcgtt aagttcgccg tcgccatggc cgcgatctca caacccgaca cgtcgcacgc
     4441 cagttcgctg ttcctcaacc gcgaactggc c
//
LOCUS       DATGOU010000054        63274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_1455156, whole genome shotgun sequence.
ACCESSION   DATGOU010000054 DATGOU010000000
VERSION     DATGOU010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 63274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63274
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455156"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VKZ48_02850"
     CDS             complement(<1..217)
                     /locus_tag="VKZ48_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013433768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76825.1"
                     /translation="MNEELRPEDEHHKPANGNSDYNSSSIKMLKGLEAVRKRPGMYIG
                     DTSDGTGLHHMVFEVVDNAVDEALAGHC"
     gene            complement(283..1392)
                     /gene="dnaN"
                     /locus_tag="VKZ48_02855"
     CDS             complement(283..1392)
                     /gene="dnaN"
                     /locus_tag="VKZ48_02855"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HLU76826.1"
                     /translation="MNLLKTNRETLLKPLQAVSGIIERRHTLPILSNVLIERDTARLS
                     FIATDIEIEIAASVDVDSGGETRAITVGARKLLDILRALPDGSEVTLALTDKRLQVKS
                     GKSRFNLQTLPAEDFPRLAGPEDASVQFELPQKLLKDLFSQVQYAMAQQDIRYYLNGL
                     LMVIDAGQLRVVATDGHRLAFASNAVADKELGKQEVILPRKTVLELAKLLADNDDPVK
                     IELSATQARFAFGDILLASKLVDGKFPDYQRVIPTQHPKELHLDRVALQQALQRAAIL
                     TSDKFRGVRWVLTENSLKVSCSNTEQEEAQEELDVAYQGEALDIGFNVGYLLDVLNNL
                     DVNEVNVALGDSNSSALITVPGRDDFKYVVMPMRI"
     gene            complement(1591..2982)
                     /gene="dnaA"
                     /locus_tag="VKZ48_02860"
     CDS             complement(1591..2982)
                     /gene="dnaA"
                     /locus_tag="VKZ48_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HLU76827.1"
                     /translation="MEVFWHHCLARLEKELPAQQFTTWIRPLQVDALRTTEDELCLTA
                     PNRFVLQWIKDRFLTRIETLAGEQLGKPVKVCVALAVNEPVSTGQGASAANEVASEPV
                     SHAAGPAVAAPVRAAPSPNVEARSNLIGDFTFDNFVPGKANALARAAAMQVAENPGTS
                     YNPLFVYGGVGLGKTHLIHAVGNHVMSEQPGAKVRYLHAAQYISDVVRAYQQRSFDAF
                     KRYYHSLDLLLIDDIQFFTGKDRTQEEFFYAFNALIEARKQIIITCDTYPRDISGIDE
                     RLKTRFSWGLTVAVEPPEQEMRVAIILKKAEALNARLPEEVAFFIAKHIRSNVRELEG
                     ALKNVLAFSRFNGKEVSVEVAKEALKDLLSVHNRHISIDNIQKTVAEFYKIKVADLHS
                     KKRTRAIARPRQMAMALAKELTEMSLPEIGDAFGNRDHTTVLHACRTIATLRTKDHEL
                     NRDFHVLEQTLKG"
     gene            3153..3287
                     /gene="rpmH"
                     /locus_tag="VKZ48_02865"
     CDS             3153..3287
                     /gene="rpmH"
                     /locus_tag="VKZ48_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HLU76828.1"
                     /translation="MKRTYQPSVVRRKRTHGFLVRMRTRGGRAVIRARRAKGRARLGV
                     "
     gene            3333..3623
                     /gene="rnpA"
                     /locus_tag="VKZ48_02870"
     CDS             3333..3623
                     /gene="rnpA"
                     /locus_tag="VKZ48_02870"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HLU76829.1"
                     /translation="MAAALRARKPVRMASLALYCRENQMDCARLALIVPKKMVPTAVA
                     RNRVRRLAREAFRLEQSHLSGLDCVVRVTRPLGDSALQLADLRALFKRAVDA"
     gene            3616..3825
                     /gene="yidD"
                     /locus_tag="VKZ48_02875"
     CDS             3616..3825
                     /gene="yidD"
                     /locus_tag="VKZ48_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HLU76830.1"
                     /translation="MLKVLLGLIHCYRYCLSPLLGRNCRFAPSCSEYAAEALQRHGAL
                     RGVWLAVRRIARCHPWHPGGYDPVP"
     gene            3841..5499
                     /gene="yidC"
                     /locus_tag="VKZ48_02880"
     CDS             3841..5499
                     /gene="yidC"
                     /locus_tag="VKZ48_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HLU76831.1"
                     /translation="MDTRRLILLVIFSFSLLMLWDAWNQQHRPPVPPQQDAAVAQQHQ
                     ADAPGVPVPSLPAAAPDAVPVAPQAVEAAPREPIRVRTDLLEAEIDPLGGNIVRLVLN
                     KYPAGSEESAPLELLAPAQRYAAQSGLIGPNLPTHRSMYQAQAQTYTLAEGQENLQVR
                     LETSVGEGVRAAKILTFHRDSYLIDVTHELVNQSAEPIAPAHAYFQLTRSGETPGRGF
                     FMAQAYTGPAVYTEQDKYRKVPFSDIAEGDASFPRTASDGWVAMVQHYFVSAVLPPAD
                     TPREYYARSIGPDLYAAGAIVPIPAVAPGSSARVNVPLYAGPQEQERLHAAAPGLDVV
                     VDYGWLTIIAEPLFWILKWFHSWLGNWGLAIIGLTVLIKLVFFPLSAASYKSMAKMRV
                     VAPRLQKLREQYGKDRAKMNQAMMELYKTEKINPLGGCLPILVQIPVFIALYWVLLGS
                     VELRNAPFYGWITDLSEQDPYYILPLLMMVSMFVQTRLSPTPPDPVQAKVMLAMPLIF
                     GVFFFFFPAGLVLYWLVNNILSIAQQWQITRMIEGGQGKTASAK"
     gene            5489..6826
                     /gene="mnmE"
                     /locus_tag="VKZ48_02885"
     CDS             5489..6826
                     /gene="mnmE"
                     /locus_tag="VKZ48_02885"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HLU76832.1"
                     /translation="MPSDADTIAAIATAAGRGAIGIVRVSGKGLADVAKTLSGAFPAP
                     RQASFARFRDADGSIIDEGLLLYFPAPYSFTGEDVLELHAHGGPVVLQLLLRRCLALG
                     ARLAEPGEFTRRAFLNDKLDLAQAEAVIDLIDAANEAAVRSAGRSLRGEFSKEIEKLV
                     EGLISVRMHVEATIDFPEEEIDPADRQTIRAGVDRLALAVGEILGRAQQGSLLRTGLN
                     VVLAGQPNVGKSSLLNRLAGEELAIVTEIPGTTRDVVRQTIYLEGLPVNIIDTAGLRE
                     TEDAVEQIGIARAWKEIEQADLLLLLVDARSGVGAEDEDILRRLPASLRRLTVYNKID
                     LVDASALPDEADDMLCVSAKTGQGVPALRERLEAIAGWQGGSEDLYLARERHLVALRE
                     AREALARAQAADPHWELLAEELRLAQAALSRITGAFTADDLLGEIFSRFCIGK"
     gene            7096..8991
                     /gene="mnmG"
                     /locus_tag="VKZ48_02890"
     CDS             7096..8991
                     /gene="mnmG"
                     /locus_tag="VKZ48_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006408094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HLU76833.1"
                     /translation="MQFPTRFDVIVVGGGHAGTEAALAAARMGCNTLLLTHNIETLGQ
                     MSCNPSIGGIGKGHLVKEVDALGGAMALAADEAGIQFRILNASKGAAVRATRAQADRV
                     LYKQAIRSRLENQPNLTLFQQGVDDLLLQGDRVAGVRTQMGLVFHGETVVLTAGTFLA
                     GLIHIGLENYAAGRAGDPPAISLAARLRELALPVGRLKTGTPPRLDGRTIDFSVMTEQ
                     PGDEPVPVFSFMGLADMHPRQLSCWITHTNERTHDIIRAGLDRSPMYTGVIEGVGPRY
                     CPSIEDKIHRFAGKSSHQIFLEPEGLQTNEVYPNGISTSLPFDVQLELVRSIAGLEQA
                     HILRPGYAIEYDYFDPRALRSSLETKAISGLYFAGQINGTTGYEEAAAQGLLAGLNAA
                     LKVQERDAWCPRRDQAYLGVLVDDLITRGVSEPYRMFTSRAEYRLMLREDNADMRLTE
                     IGRSLGLIPDDRWDAFARKRDAIAAEQQRLCSTWINPRVVDDATATEILGKPLEREHT
                     LQELLKRPEVDYERLHRLPGAGAPHPDPDVAAQVEIQVKYAGYIARQQEEVARQAQYE
                     STPLPANLDYRSVKGLSAEVQQKLNEHRPETIGQAARIQGVTPAALSLLLVHLKRATG
                     KAQRSAA"
     gene            8988..9611
                     /gene="rsmG"
                     /locus_tag="VKZ48_02895"
     CDS             8988..9611
                     /gene="rsmG"
                     /locus_tag="VKZ48_02895"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008917928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HLU76834.1"
                     /translation="MSQRDILATGVRALDLALSDQQLDALMDYVGLLQKWNRVYNLTA
                     VRDPAAMIPHHLLDSLAVVAHIPAGALLDVGSGPGLPGIPIAIAQPQRKVDLLDSNDK
                     KSSFARQAAGTLGLANVSVHCLRVEHWPGQARFPVIISRAFAELRQFVTWCAPLLADD
                     GVLAAMKGVYPETELRDLPAGFGVREAIRLDVPGLNAERHLLLIGKL"
     gene            9608..10405
                     /locus_tag="VKZ48_02900"
     CDS             9608..10405
                     /locus_tag="VKZ48_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011299482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HLU76835.1"
                     /translation="MSRTLVITNQKGGVGKTTTSVNLAAGLAEAGKRVLLVDLDPQGN
                     ATMGSGIEKRGLTHTVYHVLLGLATVEQARARAESGGYDVLPANRDLAGAEVEMVDIE
                     RRETRLKGALAAVADQYDYVLIDCPPALSLLTLNGLVAADAVMIPMQCEYYALEGLSD
                     LVNTIKKVRAQLNPRIEIEGLLRTMFDPRNTLAQQVSAQLQQHFGDKVYATVIPRNVR
                     LAEAPSYGTPAIQFDRTCKGSQAYLALAEEILRRAQAAAARAAAAQV"
     gene            10417..11289
                     /locus_tag="VKZ48_02905"
     CDS             10417..11289
                     /locus_tag="VKZ48_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012402383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HLU76836.1"
                     /translation="MNKPKAKGLGRGLAALLDRDDPVVPAAGDALATLPLDQLQPGKY
                     QPRTHMDQESLEALAASIKSQGLMQPILVRRIAAERYEIIAGERRWRAARIAGLSDVP
                     VVIREVPDQAALAMALIENIQRENLNPLEEAQGIQRLITEFKLTHQEAADAVGRSRAA
                     TTNLLRLLNLSAQVQQLVFENKLDMGHARALLALEGHRQAELAQRAAEENWSVRETER
                     RVQALLNPKLDAPKPVARKNRDVERLQEELSERLGTTVELKPGKKGMGKLIISYMNND
                     HLEELLQQLRPATA"
     gene            complement(11296..11673)
                     /locus_tag="VKZ48_02910"
     CDS             complement(11296..11673)
                     /locus_tag="VKZ48_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HLU76837.1"
                     /translation="MPRSRKISPLPKFDCGKCPGYCCSYEIPVNRRDVTRLAAHFGIS
                     VDVAERRYTKLFEGEPVLRHRKDHIYKSMCLFFDRDERRCTIYKARPAICRTYPSGTR
                     CGYYEFLVFERDQHGDEDFIPSA"
     gene            11805..12128
                     /locus_tag="VKZ48_02915"
     CDS             11805..12128
                     /locus_tag="VKZ48_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76838.1"
                     /translation="MRKFLVIAGTLAAVALAPQAAHAHNDRVGVFVAGAVTGAIVTHV
                     LTDRGHHHVYHHRSPRVVEHHYHHYPPRHRVREVHHHHPKHWGSAKHHGPRGHARHAS
                     HRHYR"
     gene            complement(12125..12610)
                     /locus_tag="VKZ48_02920"
     CDS             complement(12125..12610)
                     /locus_tag="VKZ48_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HLU76839.1"
                     /translation="MAIDTIDHKSPAEAVRLRAVLDTNVLVSLLVYRDARYARIWSAW
                     AAGQVLVVANEAVAAELGRVLARPEFAARCAPEEALAAYRARVQMVEDRPATAGLPRC
                     ADADDQKFLELAESADAHVLVTEDKALLRLRRRCRFAIERPAAFLERLPAVVTITSTT
                     S"
     gene            12697..13068
                     /locus_tag="VKZ48_02925"
     CDS             12697..13068
                     /locus_tag="VKZ48_02925"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HLU76840.1"
                     /translation="MTTPERSAGGSIRRIIGLQAGVGVVITLLLLALYGIHIALSALI
                     GGAIAVIASGVYAWRMSVPPGSDPRRMLRAHFAAEYFRFGVTVSLFAGVFLLYGSVAA
                     LPLFLTYVAALLVYWVALILN"
     gene            13087..13878
                     /gene="atpB"
                     /locus_tag="VKZ48_02930"
     CDS             13087..13878
                     /gene="atpB"
                     /locus_tag="VKZ48_02930"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HLU76841.1"
                     /translation="MATGSTHTSTEYISHHLVNWTVGEGFWTFHLDTIVVSGVIGLAI
                     FFAMAIAARKATSGVPGNFQNLVEMTLGMVDNQVKDTFHGKSELVTPLAITIFVWVWL
                     MNALDLIPVDFIPMAVMGVTGLEYFKAVPTTDPNLTFAMSLTVFVLMIAYNFKFKGVG
                     GFLREALTVPFGKWMFPINLLFRIIEDIAKPISLALRLFGNMYAGEMVFILLAVWAGA
                     SYLWVPFSSLLSLGWALFHILIITLQAFVFMMLTIVYMSMAAESH"
     gene            13918..14184
                     /gene="atpE"
                     /locus_tag="VKZ48_02935"
     CDS             13918..14184
                     /gene="atpE"
                     /locus_tag="VKZ48_02935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HLU76842.1"
                     /translation="MEALSVVLGNTALAVAILIGAGAIGTAIGFGILGGRFLEGSARQ
                     PEMIPTLQVKMFIVAGLLDAVPMIGVGIALYLLFANPFIALVQG"
     gene            14248..14718
                     /locus_tag="VKZ48_02940"
     CDS             14248..14718
                     /locus_tag="VKZ48_02940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HLU76843.1"
                     /translation="MNINATLLAQAVWFGFFIWITMKYVWPYLQRAMAERQKQIADGL
                     AAAERGKQDLVAAERRADEALNEARGRATEIIAQAEKRATQIVEEAKTAAKAEGERLL
                     AAAKAEVAQEVSRVKEDLREQVASLAVAGAEKILRREIDVKVHAQMLSQLKQEL"
     gene            14725..15264
                     /locus_tag="VKZ48_02945"
     CDS             14725..15264
                     /locus_tag="VKZ48_02945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014895498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HLU76844.1"
                     /translation="MAEIATLARPYAEAVYKQALDAGRLDEWSTALGALSAAASTPEL
                     LEVLGDPRLTDAQLLELMQGAATGPIAGEVRNLVQLLVDNKRLELLPQIRQLYEALKH
                     EHEGVVDAHIVSAFPLDEAQLRNLVNDLEARFKLKVNAEVVVDESLIGGATIQVGDAV
                     IDASVRGKLAVMSAELARI"
     gene            15290..16834
                     /gene="atpA"
                     /locus_tag="VKZ48_02950"
     CDS             15290..16834
                     /gene="atpA"
                     /locus_tag="VKZ48_02950"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012354280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HLU76845.1"
                     /translation="MQQLSPAEISDLIRSKIQNLALSADVRNQGTVVSLTDGICRIHG
                     LSDVMQGEMLEFPGNTYGLALNLERDSVGAVILGEYTHITEGDTVKSTGRILSVPVGP
                     ELIGRVVNSLGQPIDGKGPINAAETDVIEKVAPGVIWRKSVSQPVQTGLKSVDSMVPI
                     GRGQRELIIGDRQTGKTAVAIDAIINQKGKDLTCIYVAIGQKASTIANVVRKLEEHGA
                     MEYTIVVAASASESAAMQYIAPYSGCTMGEYFRDRGQDALIVYDDLTKQAWAYRQVSL
                     LLRRPPGREAYPGDVFYLHSRLLERAARVNEQWVERFTDGKVKGKTGSLTALPVIETQ
                     AGDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKIIKKLGG
                     GVRLALAQYRELAAFAQFASDLDEATRKQLERGRLVTELMKQPQYQPLDVWEIALTLF
                     AVNNSYFDDIDVNKALAAEKSLREYVRSKYAALVENIEKTKDLSADDEAKLHEAIKDW
                     QQNGSY"
     gene            16919..17782
                     /gene="atpG"
                     /locus_tag="VKZ48_02955"
     CDS             16919..17782
                     /gene="atpG"
                     /locus_tag="VKZ48_02955"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004195831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HLU76846.1"
                     /translation="MAGSKEIRTKIKSVQNTRKITKAMEMVAASKMRKAQERMRMARP
                     YGEKIRNVVAHISHANPEYRHPFLVEHDSVKRIGIILVTTDKGLAGGLNTNVQRLVLN
                     KMKEWEAEGESFEVCAIGNKGLGFMQRLGANVVSNVTQLGDKPQLEKLIGAVKIMLDG
                     YIEGRFDRVVLFYTKFINTMKQEPVAEQLLPLSGERVGLPEGTWDYIYEPDARAVLDQ
                     VLTRYIEAIIYQALCENMASEQSARMVAMKAASDNAGNVIDELTLVYNKARQAAITKE
                     LSEIVGGAAAV"
     gene            17827..19236
                     /gene="atpD"
                     /locus_tag="VKZ48_02960"
     CDS             17827..19236
                     /gene="atpD"
                     /locus_tag="VKZ48_02960"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HLU76847.1"
                     /translation="MAQAQGTIVQCIGAVIDVEFPRESMPKVYDALVLAEDNADSFAE
                     KGLTFEVQQQLGDGIVRTIALGSSDGLRRGMKVAATGNPISVPVGTATLGRIMDVLGR
                     PIDEAGPIATEERRAIHQPAPKFDELSPSVELLPTGIKVIDLICPFAKGGKIGLFGGA
                     GVGKTVNMMELINNIAKSYGGYSVFAGVGERTREGNDFYHEMEESKVLDKVAMVFGQM
                     NEPPGNRLRVALTGLTMAEKFRDEGRDILLFVDNIYRYTLAGTEVSALLGRMPSAVGY
                     QPTLAEEMGKLQERITSTKVGSITSIQAVYVPADDLTDPSPATTFLHLDATVVLSRDI
                     AALGIYPAVDPLDSTSRQLDPQIVGEEHYSVARAVQTVLQRYKELRDIIAILGMDELS
                     PEDKLAVSRARKIQRFLSQPFNVAEVFTGSPGKIVDLKDTIKGFKMIVDGECDHLPEQ
                     AFYMVGTIEEAFEKAKTLQ"
     gene            19325..19747
                     /locus_tag="VKZ48_02965"
     CDS             19325..19747
                     /locus_tag="VKZ48_02965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HLU76848.1"
                     /translation="MANTLHVDVVSAEESIYSGEASFVALPGEAGELGIYPRHTPLIT
                     RIKPGSVRIQPADGGEEELIFVAGGILEVQPEVVTVLADTAIRGHNLDEAKAQEALKR
                     AEEARATAQDKVEIAAVEAELAMLAAQLAAIRRLRNRK"
     gene            19993..21897
                     /gene="glgB"
                     /locus_tag="VKZ48_02970"
     CDS             19993..21897
                     /gene="glgB"
                     /locus_tag="VKZ48_02970"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HLU76849.1"
                     /translation="MPMSDAPHQLSEHDVYLFREGTHAQLYEKLGCRLRDEVAHFGVW
                     APNAQRVALIGEFNGWDPDAHPLRARADHSGIWETDVPGVQPGQTYKFRIWSRHGGYV
                     VDKADPFAFYAEVAPATASRAWRMDYEWGDGEWMRQRRERNALDRPISIYEVHLGSWR
                     RDGQGNLLGYRDIAHQLAEYVQRLGFTHVELMPVTEHPFYGSWGYQTTGYFAPTSRYG
                     NPQDFMYFVDVLHRHGIGVILDWVPSHFPGDAHGLAYFDGTHLYEHADPRQGFHPEWN
                     SYIFNYGRNEVRAFLLSSARFWLDRYHIDGLRVDAVASMLYLDYGRKKGEWIPNHYGG
                     KENLEAIHFLRTLNEMVYRDFPDTQTIAEESTAWPMVSRPNYIGGLGFGMKWNMGWMH
                     DTLHYMQEDPIHRKYHHHQMTFSLIYAFNENFVLPFSHDEVVYGKRSLLNKMPGDLWQ
                     QFANLRALYGYMWGHPGKKLLFMGGEFGQRSEWNHDAALQWDLLQYPEHEGLQRWLSD
                     LNLAHQREPALHQVDFNDQGFEWIDANDSETSVYSFIRKPRDAGQPIAVVCNFTPLPR
                     ENYIIGVPHRGYWHEILNSDASLYGGSGMGNYGGLQTSPVPAHGRLHSLTITLPPLAT
                     VYFRYEGAAA"
     gene            21975..23981
                     /locus_tag="VKZ48_02975"
     CDS             21975..23981
                     /locus_tag="VKZ48_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HLU76850.1"
                     /translation="MQANAQTLPPHDGRARAVIESITPQVDNGRFPAKRVIGDLVEVQ
                     ADCFADGHDAIAVVLNWRRAGEPEWKQVAMAPLGNDRWTGCFVVDALGLYEYRVIAWV
                     DHFLSWRRDLARREDAEDIRVAALVGAQLAEEAAQRADAGDRDLLRDWAARLRQSGAP
                     QALKALSLDETFAALAMRHPDRRYACSSDPLQLRVDPVHARFGSWYELFPRSAADEPG
                     RHGTLRDVEARLPYVAAMGFDILYLPPVHPIGRINRKGRNNALVPEPDDVGSPWAIGA
                     DEGGHKSIHPDLGTLEDFRSVVASARKQKIRIALDIAFQCAPDHPYVKAHPEWFKHRP
                     DGSVQYAENPPKKYQDIYPFDFESDDWQGLWTELLSIFRFWIDQGVTVFRVDNPHTKA
                     FPFWEWCIGEIKRTHPETVFLSEAFTRPRVMHRLAKLGFTQSYTYFTWRNTKQELIDY
                     FNELAHGPGADYFRPNVWPNTPDILPEHLQYGGRPAFMARLVLAATLASAYGIYGPAF
                     EHMEHRPREPGSEEYLDSEKYQLRHWDLGRDDSLAEFIARVNAIRHENVALQDNRSLR
                     FLEIDNINLLAYAKSGVNGDSLIVTVVNLDYHHTQSGWIELDLQAFGMDPDASYQMHE
                     LLTGARYLWHGARNYVSLDPQRSPAQIFRVRRRVRREQDFDYFL"
     gene            23989..27348
                     /gene="treS"
                     /locus_tag="VKZ48_02980"
     CDS             23989..27348
                     /gene="treS"
                     /locus_tag="VKZ48_02980"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HLU76851.1"
                     /translation="MDQTEPNLLQQPHPAAETDDGLWYKDAVIYELHVKAFYDSNNDG
                     IGDFRGLTARLDYIQELGVNTIWLLPFYPSPMRDDGYDVADYHNVHPDYGTRQDFRGF
                     VREAHRRGLRVITELIVNHTSDQHPWFQAARRAPKGSSKRNFYVWTDDPNKYAGTRII
                     FTDTETSNWAWDEVAQQHYWHRFFSHQPDLNFDNPNVLKAVIRTMRFWLDMGVDGFRL
                     DAIPYLVEREGTNNENLPETHAVIKEIRAALDAHYKDKLLLAEANQWPEDVREYFGDG
                     DECHMAYHFPLMPRLFLAIAQEDRYPVIEIMRQTPDIPDNCQWAIFLRNHDELTLEMV
                     TDRERDYMYEIFATDPRMRLNVGIRRRLAPLMDNDRARIELMSFLLFTLPGTPVVYYG
                     DEIGMGDNIYLGDRNGVRTPMQWSFDRNAGFSRADPQRLYLPPIMDPVYGFQAVNVEA
                     QARNPSSLLNWTRRMIAMRKGFKAFGRGTLTFLEPGNRKVLAYLREYRDETILCVTNL
                     SRYPQAAELELSRFENRVPVEILGRVPFPPIGKLPYLLTLPGYGYYVFRLATDAEPPA
                     WHAQRLVHDDLPTLVLLEGWRTFFMGSGGASDVRRAIASRSREQLKREVLLPHLQTRR
                     WFAAKGQEVTDISIIEEGEWRADDGGAWLFTFARVELADAPAQVYFLPLGIAWEEGDY
                     DPAAEYGSAALARVRQKAKTGILHSAFGDPAFCRALARAMGENREVSFGHGRLYFSST
                     PVYAQIADGFDEEVRQPPTEQTNTGVFFGTRAYLKGYRRLQAGTNPELEIGRFLTVSS
                     PFPHTAPVAGALEYVAADGERVTLALLQPYVENQGNLWDYSLDYLERYLASFGVQPEP
                     VDDHADVNLHAFFLAQATRLGHRLGSLHEAFARTTGEPDFDPEPVTPADIEQWRDSVR
                     DELILTFDLLERRRASLPESMRHLADQLLALRMRLVERLDALQLPIDGLVKTRYHGDL
                     HFGQVLIAEKDYLIVDFEGEPSRPLSERRRKHSPLRDVAGMVRSADYAARTVLTRLGA
                     GAMERHTAISEAAREWRRAVSEAFIGGYMRATRHVPSVPHEPAALLALLELFIIEKAL
                     YEVRYEMDHRPDWLDVPIGGLLEIASPAAED"
     gene            27385..28620
                     /gene="glgC"
                     /locus_tag="VKZ48_02985"
     CDS             27385..28620
                     /gene="glgC"
                     /locus_tag="VKZ48_02985"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF002023.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HLU76852.1"
                     /translation="MITRNENVLAMVLAGGEGTRLSPLTIPRSKPAVPFGGRYRIVDF
                     VLSNLVNSGIYSIYVLVQYKSQSLIEHIRNGWVMAPIFPRQFVTVVPPQMREGPEWFQ
                     GTADAIYQNINLVQDARATLVAVFGADHIYRMDLTQMIVAHRERKADVSIAAVPVPRD
                     KAQGFGIMGVADDGRIVDWVEKPQDPPEMPGRPGWALASTGNYLFDADLLVGALTAAK
                     KRGEKDFGKDVIPALMKTHRVYAYDFSKNRVPGVRQYEEQSYWRDVGTLDAYYDANMD
                     TLGPQPRIQLFNRQWPLRSSNYQGPPASLFDTHVQNSHIGAGGRFGRCTLRNSVIRRE
                     VSIGDGAVVEDSIIMDYCVIAEGAHIRRAIIDRHNIIGANERIGFDREADAARFHVTE
                     SGLVVVPMAEIMSRADAYE"
     gene            28617..30773
                     /gene="glgX"
                     /locus_tag="VKZ48_02990"
     CDS             28617..30773
                     /gene="glgX"
                     /locus_tag="VKZ48_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HLU76853.1"
                     /translation="MSTRLAVQPITAVWPGKPYPPGATWDGEGVNFALFSEHSEKVEL
                     CFFDAQGRREVQRIELRERTDQMWHCYLPEARPGMLYGYRVYGPYKPEEGHRFNPNKL
                     LLDPYAMNIVGNMRWSDALFGYQIGNKREDLSFDRRDSASGMPKCKVIDPAFTWGDDR
                     RPNVPWHDMVIYEMHVRGFTMQHPDVPPPLRGTYAGLATAPVVEYLKRLGVTTVELMP
                     VHTFVDDRYLVQKGLSNYWGYNTIGFLAPEARYSASGKVAEFKTMVKTLHSAGIEVIL
                     DVVYNHTAEGNHLGPTLSFRGIDNASYYRLTAENLRYYNDYTGCGNTLDMQHPRSLQL
                     IMDSLRYWVTQMHVDGFRFDLASALARELHEVDRLGAFFDIIRQDPVLSQVKLIAEPW
                     DLGEGGYQVGNFPIGWAEWNDKYRDTMRAYWKGDGGLIAEFATRLTGSSDLYGRGGRR
                     PYSSINFIAAHDGFTLHDLVSYNDKHNEANGEDNRDGHDNNLSWNHGVEGPTDDPQIN
                     ELRERQKRNMLATLFLSQGVPMLLAGDELCRTQGGNNNAYCQDNEISWQDWTATPERA
                     QLYEFTRRMIAIRHAHPLLRRRDFFTGDATNGHTGKDIVWLKPDGTEMTSEEWHQQFA
                     RCLGVYLSGQSMTETDERGRLLEDDDFIILFNAHHEELPFVIPHFEAPSRWRVLIDSS
                     QPDSGEWTAFESGAQYPLHGRSLALLIRPRRRSVVP"
     gene            30790..32625
                     /gene="treZ"
                     /locus_tag="VKZ48_02995"
     CDS             30790..32625
                     /gene="treZ"
                     /locus_tag="VKZ48_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HLU76854.1"
                     /translation="MKRRHLMPFGTRLLDDGGTRFRLWAPSAERVDLVLAMDSGRAEL
                     PMRAVEDGWYEKVVPGTGPGARYAYRINGGISVPDPASRFNPEDVHAASEVIDPHAYT
                     WGDADWRGRPWEEAVLYELHVGTFTPAGTYAGAIEKLDHLADLGVTAIQLMPLAEFPG
                     KRNWGYDGVLLYAPNANYGRPQDLKRFIDAAHAHGLMVLLDVVYNHFGPEGNYLHAYA
                     DAFFNPRHQTPWGDAINFDGPGAHTVRDFFIHNALYWIEEYHFDGLRLDAVHAIADDS
                     QPHILREMAEAVRTGPGHDRHVHLVLENEQNQASLLGRGAEHEPLIATAQWNDDCHHA
                     FHVLLTGERDGYYQDYADKPLAHLGRCLAEGFAFQNDVSRYRRGASRGEPSVHLPATA
                     FVDFIQNHDQIGNRAFGERINRLAPPEAVEAAAACYLLAPSVPMMFMGEEYDASTPFL
                     FFCDFGPDLSASVTAGRRSEFGRFERFKDPAVQAQIPDPVDAETYERSKLRWEEVNEP
                     AHAQRLKLYRKLLALRRAHIVPRLHGMPPSGAYVAHERAGLLVHWTLGDGTRLQLNAN
                     LATQPAEEVSAPLPDTLYASPAVEDAHLGDGRLPPWSVVWSMRAA"
     gene            32638..37755
                     /locus_tag="VKZ48_03000"
     CDS             32638..37755
                     /locus_tag="VKZ48_03000"
                     /EC_number="2.4.1.25"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:NF011077.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HLU76855.1"
                     /translation="MREALTRLARLHGIAPNYHDIWGTHHQVSDESLVALLGAIGVRA
                     DTPEAVDVALQEATHERWTHIVPPVLVLRAGAQPWRIACNVLVERLDAPVRWTLQLET
                     GEVREGRFTPSHCEEETHAHTPDGHYSRRLWQLEVDAPLGYHRLSMLRDEETLDTCAF
                     IVAPQTCYAPEALQEDGRVWGAAVQLYGVRSERNWGMGDFGDLQTLIEEWGQRGAGII
                     GLNPLHALFPHNPWHDSPYSPSSRLYKNVLYLDVEAIEDFRECEATRALVYGAAFQAR
                     LNALRDAEQVDYPGVAAAKFEILERLYAGFCERHLAHGTDRAAAFAEFRRRGGESLRR
                     HVLFEALQEHFHRHNPDVWGWPVWPEAYRHPDLPAVAQFEREAQARIGFYAWLQWQAD
                     LQLAGAGKRAHDLGLGVGVYEDLAVSINRGGAEAWANQHLYALTASVGAPPDALGPKG
                     QNWGLPPLIPERLQAEAYRPFIATLRATMRHAGAVRIDHVMGLSRLFWVPPGREATDG
                     AYMHYPFDELLGVLALESHRNRCLVIGEDLGTVSDEVREALARHRVLSYRLLYFERRD
                     GGDFKSPGEYPREAIVSVGTHDLPTLTGWWEGRDIALRTELDLFPDEATRERQHVERS
                     QDRARLLLALEREGLLPEGVTPDPSSAPQMTPELVRAVSAYLADTPSRVLVLQLEDVL
                     CVRDQANLPGTTDEHPNWKRKLPLTLERMRDDPRFIELCRTVGTKRESHRPKPRARSE
                     IEAVIPRATYRLQLHREFNFDDATRILLYLNELGVSHVYCSPYLRARSGSTHGYDIID
                     HNTLNPEIGDEAAFERFVTALARHGMGQILDMVPNHMGILGADNAWWLDVLENGQASL
                     YADYFDIDWHSLDPALRGKVLVPVLGDHYGIVLERGELRLAFDAGAGAFVVQYWDHRF
                     PIDPQQYPALLHAAAQNLAPHALSAEAHAEWQTLATAFGHLPARDTQDAAALAERQRD
                     KDVHKSRLAELVQSHALLMEAVERVVRQYNGTPGERASFDALDALLEAQAYRLAYWRV
                     ATDEINYRRFFDINDLAALRMENPATFEATHRFVLELCAAGKVDGLRIDHPDGLYDPA
                     EYFRRLQTRYAQLAGAEAESAPARKPLYLVIEKITAPHEDLPQDWPVHGSTGYRYANV
                     LNGVFVDTTAQSAMTRAWSAFVGEDAVPFEESAYRGKRLIMQTALAAELTVLSNRLLR
                     IARKDRRTRDFTLNTLRQALTEVVACFPVYRTYIVESASTQDQRYVDWAVSQAKRRIP
                     AAEDSIFEFIRSVLLLQPPEDADEALIERYRATAMRFQQYTAPVTAKGVEDTAFYRYN
                     RLVSLNEVGGDPHQFGMTVKAFHGASAARAQRWPHTMLASSTHDNKRSEDVRARLNVL
                     SEMPANWRLALRRWSRINRAKRGRMDGQPVPSRNDEYLLYQILLGSFPAGTMDADGLA
                     VYRERIERYMTKAMREAKRHTSWVNVNEEYERGVTAFVQALLGRPDDNLFLDDFLALA
                     RPLAWYGALNSVAQTLVKLCSPGVPDIYQGNETLDYSLVDPDNRRPVDYDHRARLLRD
                     AQTMAGRQDLATAARELLQPEHIHDGRAKFWVVWRALQLRREWETLFRQSDYLPVQAQ
                     GERAAHLLAFARRHEEAGVICVVGRLYASLGLEVGEPPLGERAWGDTALDLSFLPPQT
                     RLTNVLTGEMLRCRDGQLPVASLFANFSAALLRYGEA"
     gene            37773..38963
                     /locus_tag="VKZ48_03005"
     CDS             37773..38963
                     /locus_tag="VKZ48_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 - glycopeptide
                     alpha-N-acetylgalactosaminidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 100 family protein"
                     /protein_id="HLU76856.1"
                     /translation="MLYFSTPVEAQLVERCRKRSIELLKRNLSPAGILAATPGERAEA
                     RSYTAIFARDAAICALGMALSGDPALEREASTGMLTLAAHQADNGQIPKFVDVRQSTA
                     DFWYLGCIDATLWWLLIIALLDELQGSTLRKRLQAQVRQAIVWLECQEHQHFRLLQQN
                     EASDWADIMPRSGFVLYTNALWYVVKRLYDLPHADATRRNANQLFYPFSGHVAEYRRA
                     RLLSHYSRRNARNQELYLSFVNFAFYGDEGDVFGNVLAILFGLADESDAHRIIKALER
                     AAVHEPYPVRVVCDPILEASFLWRPYMSRHQQNFAWQYHNGGIWPFVGAFWVAALSAT
                     GLNDAARRELVKLARANEVGDWAFNEWLHGRTYEPGGMDGQSWNAGAFLFAHKVLETG
                     GRWF"
     gene            39055..39873
                     /locus_tag="VKZ48_03010"
     CDS             39055..39873
                     /locus_tag="VKZ48_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLU76857.1"
                     /translation="MEWWSQIAWHLVPAYVLLGIVAGFLAGLLGIGGGGMMVPVLVWL
                     FAEQGYPHEHIVHMGVATAMATVIFTSLSSVRAHARRGSVRWDIARAMAPGMLVGGFL
                     GALVADRFSTFGLGLFFVVFVYIMATNMVVDRKPAPGRSTPGNAGLFVVGTMIGVLSA
                     LIAIGGAMMTVPFLIWCAVPVIQAVGTSSAIGFPVALASTVGYMWVGANASTPATAWG
                     YVDLPAMASIATASVLLAPLGARFAHRLPTRTLRRAFAVFLFLLATRMLFQIWE"
     gene            complement(39985..40875)
                     /locus_tag="VKZ48_03015"
     CDS             complement(39985..40875)
                     /locus_tag="VKZ48_03015"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLU76858.1"
                     /translation="MKRTATLLAGAALICAGCQTVPSAANLELQGARQAYHTAQDDPY
                     VQKYGPVELERARTTLQHAEAEWRDNGNSELATHLAYVARQRALTATEVGVRGHAEDR
                     IRFVSQQRDQLLAQAQTRDLDLARMQANDAQAQALSAEQRAARLQAQLAELQAQQTER
                     GLIVTMADVMFDVDSARLRPGASRMLDRIAAVLHDDPQRRVLIEGYTDSQGQDAYNLE
                     LSARRAGAVRQALVDRGISADRIDAQGLGEAYPVATNATTAGRQLNRRVEILFSDATG
                     HIASRPLHGSYGSQPSAPMR"
     gene            complement(40872..41255)
                     /locus_tag="VKZ48_03020"
     CDS             complement(40872..41255)
                     /locus_tag="VKZ48_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HLU76859.1"
                     /translation="MRHRLCAGGVAMGAALLLAGCAVTPAAPTAQLAASRTALDQARQ
                     AGAPQSAAIEYSTARDKLARAEAAFEAQDYTAAIRLSREAEVDAQLAQARAQSTRSQH
                     ALAELEQSVRALQAELDRTHRGGGS"
     gene            complement(41316..42815)
                     /locus_tag="VKZ48_03025"
     CDS             complement(41316..42815)
                     /locus_tag="VKZ48_03025"
                     /inference="COORDINATES: protein motif:HMM:NF005702.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA synthase"
                     /protein_id="HLU76860.1"
                     /translation="MNAYTYFARAFALRLPETVIECDHGERYTYADLDRESARFAAYL
                     TGLGLRRGDRVAVQVDKSPQALFLYLACLRAGLCYLPLNTAYQRGELHYFLQDAKPGL
                     IVCRPSTLDAIRELAAETGTPHVHTLHDDATGTLIDAARDAPDRYDDVELDDDALAVI
                     IYTSGTTGRSKGAMVTHGNLSSNAQALTDGWAFSSTDVLLHALPIFHIHGLFVAFHTV
                     LLSGARMLLQRRFDAGAVLRALPDATVLMGVPTFYTRLLGEPGLTREAVQHMRLFVSG
                     SAPLLPATFEAWRARTGHVILERYGMSEAGMITSNPYAGERRCGTVGKPLPGVQLRVA
                     DDTDAPLPVGTPGMVQIKGPNVFAGYWGMPEKTREEFTADGWFRTGDIGQLDADGYLA
                     IVGRAKDLIISGGYNVYPKEIELALDALPGVIESAVIGVPDADYGEAVVAAVVKNADC
                     ALDERGIVDALRTQLAGYKLPRRVHFVDELPRNAMGKVQKAALRERYGR"
     gene            complement(42909..43121)
                     /locus_tag="VKZ48_03030"
     CDS             complement(42909..43121)
                     /locus_tag="VKZ48_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HLU76861.1"
                     /translation="MSMDEESELSLQDRLILLRQEHRELDEHIRTLGMQPGCDQLQLQ
                     RLKRKKLLLRDKIEWIERQLDPDVPA"
     gene            complement(43118..43924)
                     /gene="lgt"
                     /locus_tag="VKZ48_03035"
     CDS             complement(43118..43924)
                     /gene="lgt"
                     /locus_tag="VKZ48_03035"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HLU76862.1"
                     /translation="MLVHPQFDPIAFSIGPLSVRWYGLMYLVGFFGAYLLGRWRIARG
                     RTGAITQQVLDDLLFFAVLGVVLGGRLGYVLFYKPGHYLAHPAEIIAVWQGGMSFHGG
                     FLGVLIAVWWVARKYRLRWLDLTDFLAPLVPVGLATGRLGNFINGELWGRAASPDLPW
                     AMVFPMADATPRHPSQLYQFGLEGVALFVLLWWYSSRPRPTGAVSGMFLIGYGVFRFI
                     AEFYREPDDFLGLLTFNMSMGQWLSAPMIVAGAALWWSSRRGAVHARRNE"
     gene            complement(44020..44916)
                     /locus_tag="VKZ48_03040"
     CDS             complement(44020..44916)
                     /locus_tag="VKZ48_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLU76863.1"
                     /translation="MELRHLRYFVTVAETLNFSRAAALLHISQPPLSRQIHALEAELG
                     TPLFARTRRGVQLTDAGASLLPEARRLLRDAEALRVGARHIASGEIGKLSLGFISTAA
                     YNILPELLPAFHRAHPRIKLQLQEATSDVQVAALRDGEVDIGFVLPQPLEPTLRYEAV
                     YREPLIAALPATRRWPTKLSLTALADEAFVLFPRKVGSPLYDLMMGYCQRAGFTPRIE
                     QEAIQMQTIVSLVAAGMGVALVPASLKHLKRTGVIYRPLQERSPLMEIGLVWRANDDS
                     PAVAAFIAAARTCARAMMAKRA"
     gene            45022..46716
                     /gene="ilvD"
                     /locus_tag="VKZ48_03045"
     CDS             45022..46716
                     /gene="ilvD"
                     /locus_tag="VKZ48_03045"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012762592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HLU76864.1"
                     /translation="MADDKQTPANWRSRLITQGIARSPNRAMLRAVGFGDGDFEKPIV
                     GVANGHSTMNPCNAGIQPLVDRAIAALKAAGAMPQMFGVPTVTDGIGMGTEGMKYSLV
                     SREVIADTIETSVNGQSMDGALIVGGCDKNMPGGMMALARMNVPGIYVYAGTIKPGKW
                     KGQDLTIVSPFEAVGAYSAGKMSEEDFIGIEKHACPTVGACGGMYTANTMSSSFEALG
                     MSLLGSSQMASPDAEKADSAAASAEVLVNAIRKQIRPRDIITRKSIENAVALIMATGG
                     STNAVLHYLAIAAAAEVEWTIDDFERMRRKVPVLCDLKPSGKYVATDFHKAGGVPQVL
                     KMLLVHGLLHGDCLTITGRTIAEELADVPDAPRADQEVIRQWDNPLYPHGHLAILKGN
                     LAPEGCVAKITGLKNPSITGPARVFNSEPECMEAIMAKKIKAGDVIVIRYEGPKGGPG
                     MQEMLAPTSAIIGQGLGESVGMITDGRFSGGTWGMVVGHVAPEAFVGGTIALVEEGDS
                     ITIDAHKQLIQLNVSDEELAKRRTAWKQPPARYTRGLLAKYVKLVSTASKGAITDS"
     gene            46849..47502
                     /locus_tag="VKZ48_03050"
     CDS             46849..47502
                     /locus_tag="VKZ48_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HLU76865.1"
                     /translation="MLMTLPSAARDFAQFIGDRRFGAILADPPWQFQNRTGKVAPEHK
                     RLTRYGTLSLEEIKGLPVADAAADVSHLYLWVPNALLPEGLSVMQAWGFQYKSNLVWH
                     KVRKDGGPDGRGVGFYFRNVTELILFGVRGKNARTLAPGRRQVNFIASRKREHSRKPD
                     EQYGIIEACSPGPFLELFARGQREGWVTWGDQASEDYRPTWNTYAHNSTHAADLFVD"
     gene            complement(47499..48275)
                     /locus_tag="VKZ48_03055"
     CDS             complement(47499..48275)
                     /locus_tag="VKZ48_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009974394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HLU76866.1"
                     /translation="MFESLANNGFEIECVSHAAAILEVDFPEAVRELERALSDIRIPI
                     TEIIGSGGGEAKGTQRLRRALHAQTWRKHHFEIKKTINGIERESTSHEVDHVRVFESG
                     VVALEIEWNNKDPFFDRDLENFKRLHAEGAISLGVIITRGSSLQDRIYQLVHRFAREH
                     DVGAYADLEALGLDPTKRQKAAVMKRVERRKNPLPFAQAWAEHFVADKFGAATTHWRK
                     LMDRIDRGVGNPCPLLLIGIPASVVTFDPDDRIDPLPDAD"
     gene            complement(48434..48784)
                     /locus_tag="VKZ48_03060"
     CDS             complement(48434..48784)
                     /locus_tag="VKZ48_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLU76867.1"
                     /translation="MKQVLIVDDEYDTRAVLEIILSAEGYETCCAAHGLEALKMIEQH
                     APDLVLTDVMMPVMDGIELCAVLRTRQHTSGIPLVLITASHNVPTQQIWDGYVPKPID
                     IPKLVQTVEALLPA"
     gene            48993..49604
                     /locus_tag="VKZ48_03065"
     CDS             48993..49604
                     /locus_tag="VKZ48_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HLU76868.1"
                     /translation="MRSILVVNPKGGAGKTTLAVNLAAGLAYAGDAVCLWDLDRQHSA
                     LQWLARRSTHLPAIGRLDERERAPRKADWLVLDSPAALHGKRLTDMLKQSHKVLVPIQ
                     PSLFDIAASSDFLKELAEEKALRKNTSIGVIGMRVDPRTRAAAQLLSFLREHDMPLVG
                     WLRDTQMYPNAAFVGESIFDQPPYLAEREVAMWGPILDWVRQR"
     gene            49651..51750
                     /locus_tag="VKZ48_03070"
     CDS             49651..51750
                     /locus_tag="VKZ48_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HLU76869.1"
                     /translation="MKFTRKKPSAMPTDAPATNRLAQETSPYLQQHATNPVDWYPWGA
                     EALHRARAERKPILLSIGYSACHWCHVMAHESFEDPEVAALMNRLYVNIKVDREERPD
                     IDQIYQTAHQLFNQRPGGWPLTVFLTPEQMPFFAGTYFPKAARYGLPGFPQLLERVAA
                     YYVEHSEEIQQRSGEVQAALARTLADEEAAADELSELALDVALDMLKANFDAVNGGFG
                     DAPKFPHPVDIDFAMRRHAATGDEQAREMALTTLQKMAEGGVYDHIGGGFYRYSVDAS
                     WTIPHFEKMLYDNGPLLRVYADAWCVSNDGAFARVAEETAAWVMGEMQSPEGGYYSSL
                     DADSEGEEGRFYVWSPAEVAALLTDDEYDVVSKHYGLDGPPNFEDKAWHLRVVQPLDA
                     VAKRLQRDPAACEATLAGARAKLHAARDGRARPGRDEKVLVSWNALMIQGMAGAAAVF
                     DRPDWMASARRAMDFIRREMWRDERLLATYKDGRAHLNAYLDDYAFLLAAQLELAQAD
                     YRADDLPFAVALADALLEHFEDVERGGFHFTSHDHEALFHRPKPAHDSAMPSGNGVAT
                     FALQRLAHVTGDTRYLDAAERTLRAFYRNMAAHPSGFASLMIALTEYLEPPRTVVLRG
                     DATAIAAWRAALAREYRPSVMVHAPSGEAGTLPPGLAKPQPAEGATAYVCHGLSCLAP
                     VASLESLRAVLQTGQIQ"
     gene            51853..52107
                     /locus_tag="VKZ48_03075"
     CDS             51853..52107
                     /locus_tag="VKZ48_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLU76870.1"
                     /translation="MANPDELLKSSGCTACHAVDKRLVGPAYKDVAAKYAKEKNAEAL
                     LMEKVKKGGSGVWGPVPMPPQAHLKDENLRTMVKYILSLK"
     gene            52229..53524
                     /locus_tag="VKZ48_03080"
     CDS             52229..53524
                     /locus_tag="VKZ48_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003262401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HLU76871.1"
                     /translation="MQMRYKAALLTIIGALALYGCGQDEATSSASGDASSDDVITVVI
                     GHVAPLTGNIAHLGKDNENGARLAVDDLNAQQLKIGGKTLVIQLQGEDDQADPKTATL
                     VAQKLVDSRVAGVVGHLNSGTTIPASQIYADAGIPQVSPSATNPTYTLQGFKTAFRVM
                     ANDIQQGSVLGRYAVKGLGAKRMAIIDDRTAYGQGLADEVEKGAKAAGAEIVAREFTR
                     NDATDFRAILTSIKSRNPDVIFYGGMDAQAGPMAKQMRQLGMDAKFMAGDGGCSPEFI
                     KLAGDAAEGTYCSQTGLPLEKMPRGPAFAKRYMEKFGSPVQIYSPYSYDAVQVLVDAM
                     VRAGSIDPAKYLPEIARTSFEGVSGRVEFDEKGDLRNGAVTVYVVRNGKHEVREIVGG
                     SPIDQAAAAVRANIDAAKLAGDSAARAVGEKVRTVTPQP"
     gene            53626..54567
                     /locus_tag="VKZ48_03085"
     CDS             53626..54567
                     /locus_tag="VKZ48_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLU76872.1"
                     /translation="MIFQFDILLQQIINGLVLGSVYALVALGYTMVYGILGLINFAHG
                     DVVMIGAMTALTIATALQTFAPEMPTVLVLLVAVAGAAPVCMALGYTMERVAYRPLRN
                     APRLAPLITAIGISMVLQAAAMMIWGRNYFTFPELVSHQPIMVLGATITPVQIMIVTL
                     AAALMAGLTLMVNRTRIGRAMRATAENQPIAALMGVNVNGVISFTFIVGSGLGCVAGV
                     MVAANYGIAHYYMGFILGLKAFTAAVLGGIGNIQGAMLGGLLLGLIESLGAGYIGDLT
                     GGFLGSHYQDVFAFFVLIVVLIFKPSGLMGERVAERA"
     gene            54564..55637
                     /locus_tag="VKZ48_03090"
     CDS             54564..55637
                     /locus_tag="VKZ48_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013741788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLU76873.1"
                     /translation="MTDRFAALKAHPAAKWVGMLLIGAALLLLPFAVGHVGGPAWVRV
                     LDFALLYVMLALGLNIVVGFAGLLDLGYIAFYAVGAYTYALLASPHFGIHLPLWALLP
                     IAAGVAAIFGVLLGMPVLRLRGDYLAIVTLGFGEIIRIFLNNLNAPINITNGPQGISR
                     IDPLAVGDFTLTKSHTLFGITFSGVHWHYYFFLLLALLIIFICMRLEDSRIGRAWVAI
                     REDEVAAKAMGINTRNIKLLAFAMGATFGGVAGGLFAGFQAFVSPESFILMESIVVLC
                     AVVLGGMGNIPGVIIGALILATLPEFLRYTIMPAQQALFGQQIVDPEVLRTMLFGLAL
                     VLVMLFRPAGLWPSQVRKRELAR"
     gene            55634..56401
                     /locus_tag="VKZ48_03095"
     CDS             55634..56401
                     /locus_tag="VKZ48_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLU76874.1"
                     /translation="MTVLLEARAIDKRFGGLRALTGVSLRMERGQIYGLIGPNGAGKT
                     TFFNCLTGLYRPDGGEFRFDGEPLATDAPHEVARRGIARTFQNIRLFAHMSAIENVMV
                     GRHVRTHAGVIGAVLRGPRTRAEERAIHERAYALLDYVGLAARQHEQAKNLSYGDQRR
                     LEIARALATEPKLLALDEPAAGMNATEREALRALLCRIRDDGVSLLLIEHDVALVMGL
                     CDRIAVLDYGKTIAEGLPAEVQRDPAVIEAYLGGTLA"
     gene            56398..57123
                     /locus_tag="VKZ48_03100"
     CDS             56398..57123
                     /locus_tag="VKZ48_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLU76875.1"
                     /translation="MNAPLLAVEGLRVAYGGIQAVKGIDLEVRRGELVALIGANGAGK
                     TTTLKALMRMLPWAGGTVRYAGHDLRATPLHCMAEKGIALVPEGRGVFARLSVEENLE
                     MGAYSRRDTHAIATDMARMYELFPRLYERRDQLAGTLSGGEQQMLAMGRALMCRPQLL
                     LLDEPSMGLAPVIVQRIFEIVHEINQAGTTILLVEQNAKLALQLATRAYVMESGLITL
                     SGDAPVLARDPKVRAAYLGEVAG"
     gene            complement(57147..58187)
                     /locus_tag="VKZ48_03105"
     CDS             complement(57147..58187)
                     /locus_tag="VKZ48_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HLU76876.1"
                     /translation="MSYKVTIQPSGNTFTVEEDETVLHAALDDGFHLPYGCRNGACGS
                     CKGKLLSGEIDYGTYQETALTDEERAAGFALFCVAKPRGDLVIESREINAVKDIVVRK
                     LPCRVQKIDRVADDVVVLHLSLPANERLQFLPGQYIDFLLRDGTRKSFSLANPPHDDA
                     SIQVHIRHIPGGAFTDHVFNTMKEREIMRFEGPLGSFFLREDDDKPVVFVASGTGFAP
                     VKAILEASFQKGTTRDMVLYWGGRRPKDLYMTELAERWAAEHPNFRFVPVVSEALPED
                     DWTGRTGFVHHAVMEDYADLSGHQVYACGSPVMVESARHDFHHERGLPLEEFYADSFT
                     TAADLAKRKHVG"
     gene            58256..59119
                     /locus_tag="VKZ48_03110"
     CDS             58256..59119
                     /locus_tag="VKZ48_03110"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLU76877.1"
                     /translation="MERLLIVGCGDIARRALPQLLTRYAIRALVRSAADASALRDCGV
                     EPVIADLDCPDSLKALSEGADALVHLAPPGDEGSRDLRTRNLTAALSASGMLPRRTVY
                     ISTSGVYGDCGGRRVDETRPVNPSTPRARRRVDAEQVLTRWQPETVILRVPGIYAADR
                     LPLERLRRGTPVLRDEDDVYTNHIHADDLAAAVCRALEHVPAGAVYNISDDSELKMGE
                     WFDFVADRYGLPRPPRVSRAEASGVIPPMLLSFMNESRRLSNARMKAELGLRLRYPTV
                     REGVPGPSTEE"
     gene            59123..59455
                     /gene="cutA"
                     /locus_tag="VKZ48_03115"
     CDS             59123..59455
                     /gene="cutA"
                     /locus_tag="VKZ48_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HLU76878.1"
                     /translation="MSSPSTLLVFSNLPDRASAETLAAALVQRQAAACVNIMAPCRSV
                     YRWQGAVEQSEEIPVFIKTTADRYELVEHIVRELHPYELPEIIALPVTGGLPAYLAWV
                     AAETRPDN"
     gene            59472..61304
                     /gene="dsbD"
                     /locus_tag="VKZ48_03120"
     CDS             59472..61304
                     /gene="dsbD"
                     /locus_tag="VKZ48_03120"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HLU76879.1"
                     /translation="MNGIWRWCLLLLGLWAGMATAQEEPELLEPDRAFELSVRQVEPA
                     ALEVHYRIADGYYMYRDKFAFEAMPASVQLGQPVFPPGQVHDDEFFGRTETYRGELSI
                     RIPVSGSAERAQLRVTSQGCADIGVCYPPHRQVRTFQLAAADGGGNVGGTSAVEPAAA
                     AGSVNELLRSGRTVRNEAPMFSDEARFTDALESGRLPLIVSLFFVAGILLTFTPCVLP
                     MIPILSGLIVGEGRQVTRTNATVLSIAYVLGMVVTYTAIGVGAALSGAFFAAALQNPW
                     VLGAFALIFVALALSMFDFYDIPIPFQSHAIAASGKLPGGRIGAVAVMGMLSAAIVSP
                     CVAAPLAGALLYISQTRDALLGGSALFAMALGMGVPLIAVGVTGGALLPKSGHWMKAV
                     KKFFGFLLLGVAVWIVSPLLPVAAQMTAWATLLIVGAMFLHALDPLPAQAAGVARFWK
                     GIGVIALLVGASLLVGALSGGQSLLRPLDAVGGRVAKAAEPNVVRFERVRDVAELEAR
                     LAAATQPVLLDFYADWCVSCKEMEAFTFTDPQVRERMGQMHLLQVDVTRNSAADQALL
                     KRFRLFGPPAIIFFTAEGQETGARVIGYQPARRFQQTLDGVLNG"
     gene            complement(61305..62327)
                     /locus_tag="VKZ48_03125"
     CDS             complement(61305..62327)
                     /locus_tag="VKZ48_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HLU76880.1"
                     /translation="MRDPEEVSTMLYVDHGSCGPASVMRLVEGPPPAVRPGRVLIEVH
                     YAGVNRPDLSQRAGVYPPPPGASPILGLEVSGRVVACAPDVTQWRVGDWVCALAPGGG
                     YAEYCAVPAAHCLPVPKGLSLLEAASLPEVYFTVWANVFESAKLQAGESFLVHGGSSG
                     IGLAAIQLAKARGASTVYTTVRNAEKAAVCREYGADVVYNYRTEDWAAALWPATGKRG
                     VNVILDMVGGDYFMKNLRSLAMEGRLALIAAIRGGEIASFNAMYIIMRRLTVMGSTLR
                     ARSVEQKAGIANGLREHVWPLFESGRLRTRIHQTFPLHDVVAAHELMESSQHIGKIVL
                     AVRASH"
     gene            62400..>63274
                     /locus_tag="VKZ48_03130"
     CDS             62400..>63274
                     /locus_tag="VKZ48_03130"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HLU76881.1"
                     /translation="MDAIPQFANRTVFDGRHLLWAVVVSLMLHGLLIVGTPPLGLFDY
                     RPPIERPLELDLIAAPPPPAVVEPSHPVPPQRRAHAAPQPSEPVATTRTPRQVTAEPM
                     QAAVTQEDEAGSATAPALGEPAVPIAPIVQAAASAPAAYPLRSARLVFDLYYGDSQTL
                     VGEVVQTFELQDSRYVATASAQAVGFVGLFLHGRYEQRSEGRLGPHGMVPERYTVEER
                     RGRGTEHATFDWSAGKLRFERKGRVHEAPLEAGAQDPLSAIHQLHYLRPLEPGLLMDV
                     ATARKAERMLFMNMG"
BASE COUNT        11334 a        20050 c        20483 g        11407 t
ORIGIN      
        1 cgcagtgtcc ggccagcgcc tcgtcgacgg cgttgtcgac gacctcgaac accatatggt
       61 gcaagccggt gccgtccgac gtgtcgccga tgtacatgcc cggtcgtttg cgcacggcct
      121 ccagcccctt gagcatcttg atgctcgagg agttgtagtc cgagttgccg ttcgccggct
      181 tgtggtgttc gtcttccggg cggagttctt cgttcattcc agttcctaag aaagcgccat
      241 tcaggcgctc gtccgaatca gcgcgctagt gtcccgagat tgctagatgc gcatcggcat
      301 gacgacatac ttgaagtcgt cgcgcccggg cacggtaatc aatgcgctcg agttggaatc
      361 gcccaacgcg acattcacct cgttcacatc gaggttgttg agcacgtcga gcagatatcc
      421 gacgttgaag ccgatatcga gcgcctcgcc ctggtaggcg acatccagtt cttcctgcgc
      481 ctcttcctgt tcggtgttgc tacagctcac cttcaggctg ttttccgtca gcacccagcg
      541 cacaccgcgg aacttgtcgc tggtgagaat ggccgcgcgc tgcaaggcct gctgcagcgc
      601 cacgcgatcg aggtgcaatt ccttggggtg ctgcgtgggg atgacccgct ggtaatccgg
      661 gaacttgccg tccaccagtt tggaggcaag cagaatgtcg ccgaacgcga agcgcgcctg
      721 cgtcgcggac aattcgattt taaccggatc gtcattgtcg gccagaagct ttgccagttc
      781 gaggaccgtc ttgcggggca ggatgacctc ctgcttgccc agttccttgt cggcgacggc
      841 attggaggca aaggcgaggc gatggccgtc ggtggcgacg acgcgcagtt gtccggcgtc
      901 gatgaccatc agcaacccgt tcagataata acggatgtcc tgttgcgcca tcgcgtattg
      961 cacctgcgag aacagatcct tcagcagctt ctgcggcagt tcgaactgca cgctggcgtc
     1021 ctcgggcccg gccaggcgcg ggaaatcctc cgccggcaga gtctgcagat tgaaccggct
     1081 tttgccgctt ttgacttgca gacgcttgtc ggtcagagcc agggtgactt cgctgccgtc
     1141 gggcagggcg cgcaggatgt cgagcagctt gcgtgcaccg acggtaatgg cgcgcgtctc
     1201 accaccgctg tctacatcca ccgacgcggc gatttcgatt tcaatgtctg tcgctatgaa
     1261 ggacaggcgt gcggtatcgc gttcgatcag cacattggaa aggatcggca aggtgtggcg
     1321 ccgttcaatg atccccgaca cggcctgcaa gggcttcaac agcgtctcgc ggttggtctt
     1381 taataggttc atatcttttc ctggttattg atgatgagcg tcgcgtaaaa cggggataag
     1441 cgattgcttg cccatcggtt cagacgctta ggctgtgcgc agcctgtgga cgaacctgtg
     1501 ggtgcgtctg tgtaaatcgg aacaacttcg gtcgcactcg gaaatgcggt caagttgtgc
     1561 acagcttttg tcgggttata cataaactgt tcaacccttg agcgtctgct cgagcacgtg
     1621 gaagtcgcga ttgagttcgt gatccttggt gcgcagcgtg gcgatggtcc ggcaggcgtg
     1681 cagcaccgtg gtgtgatcgc ggttgccgaa ggcatcgccg atttccggca ggctcatttc
     1741 cgtcagttcc ttcgccagcg ccatggccat ctgccgcggc cgagcaatgg cgcgggtgcg
     1801 cttcttggaa tgcaggtcgg cgaccttgat cttgtagaac tccgccaccg tcttctggat
     1861 gttgtcgatg gagatgtggc ggttgtgcac cgacagcagg tccttgagcg cttccttggc
     1921 gacttccacg ctcacttcct tgccgttgaa acgggagaag gcgagcacgt tcttcagtgc
     1981 accttccagc tcacgtacgt tggagcggat gtgcttggcg atgaagaacg ccacctcctc
     2041 cggcagacgg gcgttcagcg cctcggcctt tttcaggatg atggccacgc gcatctcctg
     2101 ctccggcggt tcgaccgcga cggtgaggcc ccagctgaag cgggtcttga gacgctcgtc
     2161 gatgccgctg atgtcgcgcg gataggtatc gcaagtgatg atgatctgct tgcgtgcttc
     2221 gatcagcgcg ttgaaggcgt agaagaactc ctcctgcgtc cggtccttgc cggtgaagaa
     2281 ctggatgtcg tcgatgagca gcagatcgag cgagtggtaa tagcgcttga aggcatcgaa
     2341 ggagcgctgc tgataggcgc gcacgacgtc cgaaatgtat tgcgccgcgt gcagataacg
     2401 cactttcgcg cccggctgct cgctcatgac atggttgccc acggcgtgga tgaggtgcgt
     2461 cttgcccagg ccgacgccgc catacacgaa cagcgggttg taggaggtgc cgggattttc
     2521 ggcgacctgc atggcggccg cccgggccag cgcgttggcc ttgccgggaa cgaaattgtc
     2581 gaaggtgaag tcgccgatca ggttcgagcg cgcttcgacg ttcgggctgg gtgcggcgcg
     2641 caccggggcg gctacggccg ggccggccgc gtgcgatacc ggttcggacg cgacctcgtt
     2701 cgccgcggac gcgccctgtc cggtgctcac cggctcgttc acggccagcg caacgcagac
     2761 tttcaccggt ttgccgagtt gttcgcctgc cagcgtctcg atgcgcgtca ggaagcggtc
     2821 cttgatccat tgcaggacga agcgattggg cgcggtgagg cacaattcgt cttcggtggt
     2881 acgcagggcg tctacctgca aaggccggat ccaggtcgta aactgctgcg caggaagctc
     2941 tttttcgaga cgggcgaggc agtgatgcca aaaaacttcc atcctgggtt tgctcttcta
     3001 atgtccagcg cccgagcgat ggcgggagca atgcggaacg caacatcata ccctgaggcc
     3061 ccaaagttat ccacaggctg gcgatgctgt ttgacgtaag cgcaaccgat tgctttaata
     3121 gcgggttttc cgccaagccc tggatagtac aaatgaagcg cacttatcaa ccttccgtcg
     3181 tccgtcgcaa gcgtacgcac ggctttctcg ttcgcatgcg cacccgtggc ggccgcgcgg
     3241 tgattcgtgc acgccgcgcc aagggacgcg ctcgtctggg cgtttgacgc aaattgcggc
     3301 tgcgccggta tcagcgcctc ggcgctgccg atgtggccgc cgcgttgcgg gcacgcaagc
     3361 cggtgcgcat ggcgtctctg gcgctctact gccgggaaaa ccagatggat tgcgcaaggc
     3421 tcgcgctgat cgttccaaag aaaatggtgc cgacagcggt ggcccgcaac cgcgtccgcc
     3481 gtctggcgcg cgaagccttc cgtctggagc agtcgcactt gtcgggtctg gattgcgtcg
     3541 tgcgggtcac tcgcccgctg ggcgattcgg ccttgcagct ggcggattta cgagcccttt
     3601 ttaagcgggc ggtcgatgct taaagtcctg ctcggattga ttcactgcta tcgctattgc
     3661 ctgagcccgc tgctgggccg caattgccgc ttcgcccctt cctgttccga atacgccgcc
     3721 gaggcgctgc agcgccacgg tgcgcttcgc ggtgtctggc tggccgtgcg ccgcattgcc
     3781 cgttgtcatc cctggcatcc gggcggttac gaccccgtac cttgaaataa ccgataaaca
     3841 atggataccc gacgcctgat cctgctggtc atcttctctt tctcgctgtt gatgttgtgg
     3901 gacgcgtgga accagcagca tcgtccgcct gtgccaccgc aacaggatgc cgcagtcgcg
     3961 caacagcacc aggccgatgc gccgggcgta cccgttccct cgcttcccgc tgcagcgcct
     4021 gacgccgtgc cggttgcgcc gcaggcagtg gaggcggcgc cgcgtgagcc gattcgcgtc
     4081 cggacggatc tgctcgaggc ggagatcgac ccgctcggcg gaaacatcgt gcgtctggtg
     4141 ctgaacaaat accccgcggg cagcgaagag agcgcgcccc tggaactgct cgcaccggcg
     4201 caacgctacg cggcgcaaag cggactgatc gggccgaacc tgccgacgca ccggtcgatg
     4261 taccaagcgc aggcgcagac ctatacgctg gccgagggac aagagaatct gcaggtccgg
     4321 ctggagacgt cggtcggtga gggcgtgcgc gccgcgaaaa tcctgacttt ccatcgcgac
     4381 agctatctga tcgatgtcac gcatgagctg gtgaatcagt ccgccgagcc gatcgcgcct
     4441 gctcacgcct atttccagtt gacgcgcagc ggtgagacgc cggggagagg cttctttatg
     4501 gcgcaggcct acaccggccc cgccgtgtac accgagcaag ataagtacag gaaggtgccc
     4561 ttctcagata ttgccgaagg cgatgcgagt tttccccgca cggccagtga cggctgggtg
     4621 gcgatggtcc agcactactt cgtttcggcc gtcctcccgc cggcggatac cccgcgcgag
     4681 tattacgccc gctccatcgg tccggatctc tatgccgccg gtgcgatcgt gccgattccg
     4741 gcggttgcgc ccgggtcgag cgcacgcgtc aacgtgccgc tctacgccgg cccccaggaa
     4801 caggagcgct tgcatgcggc tgcgcccggc ttggacgtgg tggtcgacta tggctggctg
     4861 acgatcattg ccgagccgct gttctggatt ctgaaatggt ttcatagctg gctgggcaac
     4921 tggggtctgg ccatcatcgg gctgaccgtg ctgatcaagc tggtgttctt cccgctttcc
     4981 gcggcgagct acaagtcgat ggcgaagatg cgggtggtgg cgccgcggct gcagaaactg
     5041 cgcgagcagt acggcaagga tcgcgcaaag atgaaccagg cgatgatgga gttgtacaag
     5101 accgagaaga tcaatccgct cggcggctgc ctgccgatcc tggtgcagat cccggtattc
     5161 atcgcgctgt actgggtgct gctgggcagc gtggaactgc gcaacgcgcc cttctacggc
     5221 tggatcaccg acttgtcgga gcaggatcct tattacatcc tgcctttgct gatgatggtc
     5281 tccatgttcg tccagacccg cctcagcccg acgccgcccg atccggtgca ggcgaaggtg
     5341 atgctggcga tgccgctgat cttcggcgtg ttcttcttct tcttcccggc cggcctggtg
     5401 ctgtactggc tggtgaacaa catcctgtcg atcgcgcagc aatggcagat cacgcgcatg
     5461 atcgaaggcg ggcagggtaa gacggcgagt gccaagtgat gccgacacca tcgccgcgat
     5521 cgccacggcc gccggtcgtg gcgcgatcgg catcgtgcgc gtgtccggca aaggattggc
     5581 ggacgtcgca aagacgctga gcggcgcctt cccggccccg cgtcaggcca gctttgcaag
     5641 attcagggac gccgacggca gcatcatcga cgaaggtctg ctgctctact ttcccgcccc
     5701 ctattccttc accggcgaag acgtgctgga actgcacgct cacggcgggc ccgtggtgct
     5761 gcaactgctg ctgcgccgtt gtcttgcact tggtgcacgt ctggccgaac ccggagagtt
     5821 cacccgccgg gcttttctca acgacaagct cgatctggcg caggctgaag ccgtcatcga
     5881 tctcatcgat gcggcgaacg aagcggccgt gcgcagcgcc ggccgtagtc tgcggggcga
     5941 gttctcgaag gaaatcgaga agctcgtcga gggattgatt tcggtccgta tgcacgtgga
     6001 ggcgacgatc gattttccgg aggaggaaat cgacccggcc gaccggcaga ccatccgtgc
     6061 aggcgtggac cgactcgccc ttgcggtggg cgaaatcctg ggacgcgcgc aacagggcag
     6121 cctcctgcgg accgggctca acgtggtgct ggcggggcag ccgaacgtcg gcaagtcgag
     6181 cctgctgaat cggctcgcgg gcgaggagtt ggcgatcgtc accgagattc ccggcacgac
     6241 gcgagacgtc gttcggcaga ccatttacct cgagggcttg ccggtcaaca tcatcgatac
     6301 cgccggattg cgcgaaaccg aggacgccgt ggagcagatc gggatcgctc gggcctggaa
     6361 ggaaatcgaa caggccgatc tgctgcttct gcttgtggat gcgcgcagcg gcgtgggcgc
     6421 ggaggacgaa gacattctgc gccggctgcc ggcaagtctt cggcgcctga cggtgtacaa
     6481 caaaatcgat ctggtcgatg cgtcggcact gccggacgag gcggacgaca tgctttgcgt
     6541 ttcggccaaa acaggccaag gtgttccggc attgcgcgaa cgcctggaag cgatcgccgg
     6601 ctggcagggc ggcagcgaag acctttatct cgcgcgggaa cggcatctcg tcgcgctgcg
     6661 cgaggcgcgt gaggcgctcg cccgtgcgca ggcggccgac ccgcattggg aactgctggc
     6721 tgaggaactc cggcttgcgc aagcggcact cagccgcatt accggtgcct tcaccgctga
     6781 cgatctgctg ggcgaaatat tcagccgttt ttgcatcggc aagtgagcgg ttggctgcct
     6841 tttgtccata aatatggccg tgtgtggtgt gagtgattat tcactccaaa gtgtaagctc
     6901 gcctctttat cgaaggtgct gccggcgggc cggccgggaa gatgcggtcc gactggcacg
     6961 gtgcgtgcgg acgaaagcgc cgtaggaggc tcaaccgtag ccagtcgggt gctgcccgag
     7021 ttgtttcacg tggaaccttt ggtcgccgcc aagctctgtg gcgtatcatt cgctccctta
     7081 ccccaggttt cagtcatgca gtttcccact cgtttcgatg tcattgtggt cggcggcggc
     7141 catgccggta cggaggccgc gttggcggcc gcccgcatgg gctgcaatac cctgctgttg
     7201 acccacaata tcgaaacgtt gggccagatg tcctgcaacc cttcgatcgg gggcatcggt
     7261 aaggggcatt tggtgaagga ggtagacgca ctgggcggcg cgatggcgct cgctgcggat
     7321 gaagcgggca tccagttccg catcctcaac gcaagcaagg gtgcggcggt ccgtgcaacc
     7381 cgtgcgcagg ccgaccgggt tctctacaag caggcgattc gcagccgatt ggagaatcag
     7441 cccaatttga cgctgttcca gcagggcgtc gacgatctac tcctccaggg cgaccgtgta
     7501 gccggggtgc ggacgcaaat gggactggtg ttccatggcg aaacggtcgt gttgaccgcc
     7561 ggaaccttcc tggccggcct tatccacatt ggacttgaaa attacgctgc cgggcgggca
     7621 ggggatccac ctgcgatcag tctggcggcg cggctgcggg aactcgcgct gccggtcggc
     7681 cggttgaaga cgggtacgcc gccgcggctg gatggccgga ccatcgactt ttccgtcatg
     7741 accgaacagc cgggcgacga accggtgccc gtgttctcct tcatgggctt ggccgacatg
     7801 cacccgcgcc aattgtcctg ctggatcacc cacaccaacg agcggacgca cgacatcatc
     7861 cgggcaggcc tcgaccgctc gcccatgtat accggggtga tcgaaggagt ggggccccgc
     7921 tactgtccgt cgatcgaaga caaaattcac cgtttcgcgg gtaaaagcag tcatcagatc
     7981 ttcctggagc cggaagggct gcagaccaac gaggtctacc ccaacggaat ctccaccagt
     8041 ttgcccttcg atgtgcagtt ggagctggtc cgctccattg cgggcctgga gcaggcgcac
     8101 atcctccggc ccggttacgc gatcgaatac gattatttcg atccgcgcgc cttgcgcagt
     8161 tcgctggaaa ccaaggccat cagcgggctg tacttcgccg gccagattaa cggcacgacg
     8221 ggttacgaag aagcggccgc gcagggcctg ctggcgggat tgaacgctgc cttgaaggtg
     8281 caggaacgcg atgcctggtg tccaaggcgg gatcaggcgt atctgggtgt gctggtcgac
     8341 gatctcatca cccgcggggt atccgagccc tatcggatgt ttaccagccg tgccgagtac
     8401 cggctcatgc tgcgggaaga caatgccgac atgcggttaa cggagatcgg ccgctcgctg
     8461 ggattgattc cggacgaccg ttgggacgca tttgcacgca aacgggacgc gattgcagca
     8521 gagcagcagc gtctatgttc cacgtggatc aatccgcgcg tggtggatga tgccaccgcc
     8581 acggaaatcc tggggaaacc gctggagcgc gagcacaccc tgcaggaatt gctgaaacga
     8641 cccgaagtgg attacgagcg cctgcaccgc cttcccggcg cgggcgcgcc gcatcccgac
     8701 ccggatgtcg ccgcacaggt cgaaatccag gtgaagtacg ccggttatat tgcgcggcaa
     8761 caggaggagg tggcccgtca ggcacagtat gaatcgacgc ccctgccggc caatctcgat
     8821 taccgcagcg tcaaaggcct gtctgccgaa gtccagcaga aactgaatga acatcggccg
     8881 gaaacgatcg gccaggcggc ccgcatccag ggcgtgacgc ccgctgcgct gtcgttgctg
     8941 ctcgtccatc tgaagcgcgc aaccggcaag gcgcaacgct ccgcggcatg agccagcggg
     9001 acattcttgc gacgggcgtg cgcgccctcg atctggcctt gagcgaccag cagctcgacg
     9061 cgttgatgga ctatgtcggg ctgctccaga aatggaaccg cgtctacaac ctcacggccg
     9121 tccgcgatcc cgcagccatg attccgcacc atttactgga cagtctcgcc gtggttgcgc
     9181 atattccagc aggggcgttg ctcgatgtgg gcagcggtcc ggggctgccc ggaatcccga
     9241 tcgccattgc gcagccgcag cgcaaggtcg acttgctcga cagtaacgac aagaaaagca
     9301 gctttgcacg tcaggcagca ggtacgctgg ggcttgctaa tgtgagtgtt cactgtcttc
     9361 gtgtagagca ctggcctggg caggcgcgat ttccggtgat catttcccga gcgtttgcgg
     9421 agttgcggca gttcgtgacc tggtgtgcgc cgctgctcgc cgacgatggc gtactcgcag
     9481 ccatgaaagg cgtttacccg gaaacggagt tgcgcgacct gcccgcaggc ttcggtgtgc
     9541 gggaggcgat ccggctcgac gtccccggac tgaatgcgga aagacatttg ttattgatag
     9601 ggaagctatg agcaggaccc tggtcattac caaccaaaaa ggcggggtgg ggaaaaccac
     9661 caccagcgtg aacctggcag ccgggcttgc cgaggcgggc aagcgggtcc tactggtgga
     9721 tctcgatccc cagggcaatg ccaccatggg atccggcata gaaaagcgtg gcctcacgca
     9781 taccgtctat cacgtgctgc tgggccttgc gaccgtggag caggcgcgcg cccgggcgga
     9841 aagcggcggt tacgacgtcc tgccggccaa ccgggatctg gccggcgccg aggtcgagat
     9901 ggtggatatc gagcgacgcg agacgcgcct caagggggcg cttgccgccg tcgccgatca
     9961 atacgactac gtgctgatcg actgtccgcc cgcactgagc ctgctgacgc tcaacggcct
    10021 ggtggctgcc gacgcggtga tgatcccgat gcagtgcgag tactacgcgc tggagggcct
    10081 gtcggatctg gtcaacacca tcaagaaggt gcgcgcccaa ctcaacccgc gcatcgaaat
    10141 cgaagggctg ctgcgcacca tgttcgatcc gcgcaatacg ctggcgcagc aggtgtcggc
    10201 gcagttgcag cagcacttcg gcgacaaggt ttatgccacc gtcataccgc gcaacgtgcg
    10261 gttggccgaa gcgcccagtt acggcacacc cgccattcag ttcgatcgca cctgcaaggg
    10321 ctcgcaggct tatctggcgc tcgcggagga gattctgcgc cgcgcccagg ccgccgctgc
    10381 gcgcgcggcg gccgcgcaag tttgaggatg aaaacgatga ataaaccgaa agccaaggga
    10441 ttggggcgcg gtctggccgc gctgctcgac cgggacgacc cggtcgtacc ggcggcaggc
    10501 gatgcgctgg ccacacttcc tctcgaccag ttgcagccgg gcaagtacca gccgcgtacg
    10561 cacatggacc aggaatcgct ggaagcgttg gcggcctcga tcaaatccca gggcctgatg
    10621 cagcccatcc tggtccgccg cattgccgcc gagcgctacg agatcatcgc cggcgaacgg
    10681 cgctggcgcg ccgcgcgcat cgccggtttg tctgacgtac cggtggtgat ccgcgaagtg
    10741 cccgatcagg cggcgcttgc catggcgctg atcgaaaaca tccagcggga aaacctcaac
    10801 ccgctcgaag aggcgcaagg catccagcgc cttatcaccg aattcaagct gacgcatcag
    10861 gaagcggccg acgccgtggg ccgctcgcgt gcggccacca ccaacctgct gcgtctgttg
    10921 aacttgagcg cgcaggtcca gcaactggtg ttcgagaaca aactcgacat ggggcacgcg
    10981 cgcgcgctgc tcgcgctgga aggccatcgc caggccgagc ttgcccagcg cgccgccgag
    11041 gagaactggt ccgtgcgcga gacggagcgc cgggtccagg cgctgctcaa tccgaagctg
    11101 gacgccccca agccggtcgc gcgcaagaat cgcgacgtcg aacggctgca ggaggaattg
    11161 tccgagcggc tcggtaccac cgtcgaactc aagcccggca agaaaggcat gggcaagctg
    11221 atcatcagct atatgaacaa cgaccatctg gaagagctgt tgcagcagct acgtccggct
    11281 acggcctgag acgcttcagg cggaggggat gaagtcctcg tcgccgtgct ggtcgcgctc
    11341 gaacaccaga aactcgtaat agccgcagcg cgtgccgctg ggataggtgc ggcagatcgc
    11401 cggccgcgcc ttgtagatcg tgcaccggcg ttcgtcgcga tcgaagaaca ggcacatcga
    11461 tttgtaaatg tgatccttgc ggtggcgcag taccggttcg ccttcgaaca gcttggtata
    11521 gcgccgctcc gccacatcga cggaaattcc gaaatgcgcc gccaggcgcg tcacatcgcg
    11581 ccggttcacc ggtatctcat agctgcagca gtagccggga cacttgccgc aatcgaattt
    11641 cggaagggga gaaatcttgc gtgaacgggg catggcggtt ccttgagcag gcgcgatggt
    11701 cgcccccgct tacacgcctg taaagtgtct tacaaaccgt aacccatcgc gccgtccgtc
    11761 cagggcacgc ttgcctcact cgtaacaacc caaggaggca ggagatgcga aagtttctgg
    11821 tcattgccgg gaccctggct gcggtcgccc tcgcgcccca ggcggcacac gcgcataacg
    11881 accgtgtggg cgtgtttgtc gccggtgcgg tgaccggcgc gatcgtcacc cacgtactga
    11941 ccgaccgcgg tcaccaccac gtgtaccacc atcgcagtcc gcgtgtggtc gagcatcatt
    12001 accatcatta cccgcctcgt caccgcgtgc gcgaagtcca tcaccatcac cccaagcact
    12061 ggggctcggc caagcatcac ggcccgcgcg gccacgcgcg ccacgccagt caccggcact
    12121 atcgttagga cgtagtggaa gtgatcgtca cgacggccgg cagccgctca agaaaggcgg
    12181 ccggccgctc gatggcgaag cggcaacgcc gccgcagccg caacaacgcc ttgtcctcgg
    12241 ttaccaggac gtgggcgtcc gcagattcgg ccagctccaa aaacttctga tcgtcggcat
    12301 cggcgcagcg gggcaacccg gcggtggccg gccggtcctc caccatctgc acccgcgccc
    12361 ggtaggccgc aagggcttcc tccggcgcgc agcgcgccgc aaactccggc cgcgccagca
    12421 cccgccccag ctccgcagcg acggcctcgt tggccaccac aagcacctgg ccggctgccc
    12481 aggcggacca gattcgcgca taacgggcgt cccggtaaac cagcaaagaa accagcacgt
    12541 tcgtgtccag aacggcccgc agacgcaccg cctctgcggg gcttttgtga tcgattgtat
    12601 cgatagccaa tatcgtctga attcctgcac ttgcgatttg actcgcgttg ttaaaggccc
    12661 gtagaattct gcggttttgc ggcttggggt tgacgcatga ccacgcccga aagatcggcc
    12721 ggtgggtcca ttcgccggat catcggtctg caggcaggcg tcggcgtcgt aataaccctg
    12781 ctgctattgg cactgtacgg aatccatatt gcgttgtccg ctctcatcgg cggtgcgatc
    12841 gcggtgatcg cgagcggtgt gtatgcgtgg cgcatgtcgg ttccgccagg gagcgacccg
    12901 agaaggatgt tacgggctca cttcgccgcg gagtattttc gcttcggcgt aacggtgagt
    12961 ctgtttgcgg gggtctttct cctgtacggc agtgtcgcgg cgctgccgct gtttttgact
    13021 tacgtcgccg ccctgctggt gtattgggtg gccctgatac tgaactgacg agtaacgcct
    13081 tccgcaatgg ccaccggatc gacgcacacc tccaccgaat acatcagcca tcacctcgtc
    13141 aactggacgg taggtgaagg cttctggact ttccatctcg ataccatcgt cgtctccggt
    13201 gtcatcggcc tggccatttt cttcgccatg gccattgcag cgcgcaaggc gacctccggc
    13261 gtcccgggta atttccagaa cctcgtggaa atgacgctcg gcatggtcga caaccaggtc
    13321 aaggatacct tccacggcaa gagcgaactg gtgacgccgc tggccatcac gatcttcgtg
    13381 tgggtctggc tgatgaacgc actggacctg attccggtgg acttcatccc gatggcggtg
    13441 atgggcgtga ccggcctgga atacttcaag gcggtgccga ccaccgaccc gaacctcacc
    13501 ttcgccatgt cgctgacggt gttcgtgctg atgatcgcct acaacttcaa gttcaagggc
    13561 gtcggcggtt ttctgcgcga agcgttgacc gtgccgttcg gcaaatggat gttcccgatc
    13621 aacctgctgt tccgcatcat cgaagacatt gccaagccga tttcactggc gcttcgtctg
    13681 ttcggcaaca tgtacgccgg cgagatggtg ttcatcctgc tcgccgtttg ggcaggcgcg
    13741 agctacctct gggtgccgtt ctcgtccctg ctgagcctcg gctgggcgct gttccacatt
    13801 ctgatcatca cgctgcaggc cttcgtgttc atgatgctca ccattgtcta catgagcatg
    13861 gccgcggaat cgcactagtt ttttctatcc acctaccact ctctgaagag gataaaaatg
    13921 gaagcactct ccgtagtctt gggcaacacc gcgctcgccg tggcgatcct gatcggcgcc
    13981 ggcgctatcg gcactgcgat cggcttcggt attctcggcg gccgtttcct ggaaggctct
    14041 gcccgccagc ccgagatgat cccgacgctg caggtcaaga tgttcatcgt ggccggtctg
    14101 ctcgacgcgg tgcccatgat cggtgtcggt atcgccctgt acctgctgtt cgccaacccc
    14161 ttcatcgccc tggttcaagg ttaagcgatt gtcgattgcg gccagggatg tgttgatccc
    14221 ggccgccggg tagaacagga gactggcgtg aatatcaacg ctacattgct ggcgcaggcc
    14281 gtttggttcg gtttcttcat ctggatcacg atgaagtacg tctggccgta cctccagcgt
    14341 gcgatggccg agcgtcagaa gcagattgcc gatggcctgg ccgccgccga gcgcggcaag
    14401 caggacctgg ttgccgccga gcgtcgtgcg gacgaggcgc tgaacgaggc gcggggccgc
    14461 gcgacggaaa tcattgcgca ggcggaaaag cgcgcgaccc agatcgtcga agaggcgaaa
    14521 acggccgcca aagccgaagg cgagcggctg ctcgcagcgg cgaaagccga agttgcgcag
    14581 gaagtgtcgc gcgtgaagga agatctgcgc gagcaggttg catcgctcgc ggtggccggt
    14641 gcggaaaaga tcctgcgtcg cgaaatcgat gtcaaggtcc atgcgcagat gctgtcgcag
    14701 ctgaagcagg agctttaagc cgccatggcc gaaatcgcaa cccttgcccg cccctacgcc
    14761 gaagcggtct acaagcaggc gctcgacgcc gggcggctgg acgaatggtc gaccgcgctc
    14821 ggtgcgttgt cggccgcagc ctccacgccg gagttgctcg aggtgctggg cgatccgcgc
    14881 cttaccgacg cgcagttgct ggaattgatg cagggtgcgg ctaccgggcc catcgccggc
    14941 gaagtgcgca atttggtgca gctgctggtg gacaacaagc gcctcgaatt gctgccgcag
    15001 atccgccaac tctacgaagc gctgaagcac gaacacgagg gcgtggtgga cgcccacatc
    15061 gtgtccgcct tcccgctgga tgaggcgcag ttgcgcaatc tcgtcaatga tctcgaagcg
    15121 cgtttcaagc tgaaggtgaa cgccgaagtc gtggtcgacg agtcgctgat cggcggcgcg
    15181 accatacaag tcggcgatgc agtcatcgac gcctcggtgc gcggcaaact ggccgtcatg
    15241 tccgccgagc tggcccggat ttgaacctta aaggattcag gagtctttca tgcaacagct
    15301 tagccccgcc gagatcagcg atctgatccg cagcaagatc cagaatcttg cgctgtccgc
    15361 ggacgtgcgc aatcagggca cggtcgtttc gcttaccgac ggcatttgcc gcatccacgg
    15421 gctgtccgac gtgatgcagg gcgagatgct cgaatttccg ggcaacacct atggtctcgc
    15481 gctgaacctc gaacgcgatt ccgtcggcgc ggtgattctg ggtgaataca cccacatcac
    15541 cgaaggcgac accgtcaaga gcacgggccg cattctgtcc gtgcctgtcg gccccgaact
    15601 gatcggtcgc gtggtcaact cgctcggcca gccgatcgac ggcaagggcc ccatcaatgc
    15661 cgccgaaacc gacgtcatcg agaaagtcgc accgggcgtg atctggcgga agtccgtgtc
    15721 gcagccggta cagaccggtc tgaagtcggt ggactcgatg gtgccgatcg gccgcggcca
    15781 gcgcgaactg atcatcggcg accgtcagac gggtaagacc gccgtcgcga tcgacgcgat
    15841 catcaaccag aagggcaagg acctcacttg tatctacgtc gcgatcggtc agaaagcctc
    15901 gaccatcgcc aacgtggtgc gcaagctgga agagcacggc gcgatggaat acaccatcgt
    15961 cgtggccgct tccgcctccg aatccgccgc gatgcaatac atcgcgccgt actcgggctg
    16021 cacgatgggc gaatacttcc gcgaccgcgg tcaggacgct ctcatcgttt acgacgactt
    16081 gaccaagcag gcctgggcct atcgtcaggt gtcgctgctg ttgcgccgcc cgccgggccg
    16141 cgaagcctat cccggtgacg tgttctatct gcactcccgc ctgctcgagc gcgccgcgcg
    16201 cgtaaacgag cagtgggtcg agcgcttcac cgacggcaag gtcaagggca agaccggttc
    16261 gctgactgcg ctgccggtta ttgaaacgca ggccggcgac gtgtccgcgt tcgtgccgac
    16321 caacgtgatc tcgattaccg acggccagat cttcctggaa accgacttgt tcaacgccgg
    16381 tatccgtccc gcgatcaacg ccggtatttc ggtgtcgcga gtcggtggcg ccgcgcaaac
    16441 caagatcatc aagaagctcg gcggcggtgt gcgtctggcg ctcgcgcagt accgtgaact
    16501 ggcggccttc gcacagttcg cctcggatct ggacgaagcc acccgcaagc agctggagcg
    16561 cggccgtctg gtcaccgaac tgatgaagca gccgcagtac cagccgctcg atgtatggga
    16621 aatcgcgctg acgctgttcg cggtgaacaa ctcctatttc gacgacatcg atgtgaacaa
    16681 ggccctcgct gcggaaaaga gcctgcgcga atacgtgcgc agcaagtacg ccgcgctggt
    16741 ggagaacatc gagaagacga aggatctgtc cgccgacgac gaagccaagc tgcacgaggc
    16801 gatcaaggac tggcagcaga acggctcgta ttgaaaccgg gaacggcctt cggccgtacc
    16861 agcgagtatc acgacccccg cggtgcgggg gtcgaacttg agttgaacag gtagcgtaat
    16921 ggctggctcg aaagagatcc gcaccaagat caagagcgtg cagaacacgc gcaagatcac
    16981 caaggcgatg gaaatggtcg ccgcgtcgaa aatgcgcaaa gcgcaggagc gcatgcgcat
    17041 ggcgcggccc tacggcgaga aaatccgcaa cgtcgtggcg catatctccc acgccaaccc
    17101 ggaataccgc caccccttcc tggtcgagca cgactcggtc aagcgcatcg gcatcattct
    17161 ggtcacgacc gacaagggtc tggccggcgg tctcaacacc aacgtgcagc gtctggtgct
    17221 gaacaagatg aaggagtggg aggcggaggg cgagtcgttc gaagtgtgcg ccatcggcaa
    17281 caagggcctg ggcttcatgc agcgactggg cgcgaacgtc gtttccaacg tcacgcaact
    17341 gggcgacaag ccgcagctgg aaaagctgat cggcgcggtg aagatcatgc tcgacggcta
    17401 catcgagggc cgcttcgacc gcgtggtgct gttctatacc aagttcatca acaccatgaa
    17461 gcaggaaccg gtggccgagc aactgctgcc cctgtccggt gagcgtgtcg gcctgcccga
    17521 agggacgtgg gactacatct acgaaccgga tgcgcgagcg gtgctcgacc aggtgttgac
    17581 gcgttacatc gaggcgatca tctaccaggc gctgtgcgag aacatggcat cggagcaatc
    17641 cgcgcgcatg gtcgcgatga aggctgcgtc cgacaacgcc ggcaacgtga tcgacgaact
    17701 gacgctggtg tacaacaagg cgcgtcaggc ggccattacc aaagagcttt cggaaattgt
    17761 gggcggcgcc gccgcggttt aagcgcgcga ccgccatcga ataagttaat agtttaggaa
    17821 aacgctatgg cacaagcaca aggcaccatc gtccagtgca tcggcgcggt gatcgacgtc
    17881 gagtttccgc gcgagagcat gcccaaggtt tatgacgcgc tggttctggc ggaagacaac
    17941 gccgattcct ttgccgagaa ggggctgact ttcgaagtgc agcagcagct gggcgacggt
    18001 atcgtgcgca ccatcgcgct gggttcttcc gacggtctgc gtcgcggcat gaaagtcgcc
    18061 gcaaccggca atccgatttc cgtccccgtc ggtaccgcga ctctgggccg catcatggac
    18121 gtgttgggcc gtcccatcga cgaggcgggc ccgatcgcga ccgaagagcg ccgtgccatt
    18181 caccagcccg cgccgaagtt cgacgaactg tcgccctcgg tcgagctgct gcccacgggt
    18241 attaaggtta tcgacctgat ctgccccttc gccaaaggcg gcaagatcgg tctgttcggc
    18301 ggtgccggcg tcggcaagac cgtcaacatg atggaactca tcaacaacat cgccaagagc
    18361 tacggcggct actccgtgtt cgccggcgtg ggcgagcgca cccgcgaggg caacgacttc
    18421 tatcacgaga tggaagaatc gaaggtgctc gacaaagtgg cgatggtgtt cggtcagatg
    18481 aacgagccgc cgggcaaccg tctgcgtgtc gcgctcaccg gcctgaccat ggcggagaag
    18541 ttccgcgacg aaggccgcga cattctgctg ttcgtcgaca acatctatcg ttacaccctc
    18601 gccggtaccg aagtgtccgc gctgctcggt cgtatgcctt ctgcggtggg ttatcagccg
    18661 acgctggcgg aagagatggg taagctgcag gaacgcatta cctcgaccaa ggtcggctcc
    18721 atcacctcga tccaggccgt gtacgtgccg gcggacgact tgaccgatcc gtcgcctgcc
    18781 acgaccttcc tgcacctgga tgcgaccgtc gtgttgtcgc gtgatatcgc cgcgctgggg
    18841 atctaccccg cggtcgaccc gctcgactcc acctcgcgtc agctcgatcc gcagatcgtc
    18901 ggggaagagc actacagcgt cgcgcgcgcg gtgcagaccg tgctgcagcg ctacaaggaa
    18961 ctgcgcgaca tcatcgcgat tctcggcatg gacgaactgt cgccggaaga caaactggcc
    19021 gtgtcccgcg cacggaagat ccagcgcttc ctgtcgcagc cgttcaacgt ggcggaagtg
    19081 ttcaccggct cgcccggcaa gatcgtcgac ctgaaggaca ccatcaaggg cttcaagatg
    19141 atcgtcgacg gcgagtgcga ccacctgcct gagcaggctt tctacatggt cggcaccatc
    19201 gaagaagcct tcgagaaagc gaagaccctg cagtaagcgt ttcgatcctg cgcgccgggt
    19261 gccggcgcgc aggaggcgac catgctgaat tcccgcctgc gcggggatga catgcgagaa
    19321 tcaaatggcc aatacactac acgtcgatgt cgtcagcgcc gaggagtcca tctactccgg
    19381 cgaagccagc tttgtcgcgc tgcccggcga agcgggcgag ctcggcatct atccgcgcca
    19441 cacgccgctc atcacccgca tcaagccggg ttcggtgcgt attcagccgg ccgacggcgg
    19501 cgaggaagaa ctgatcttcg tcgcgggcgg tattctggag gtgcagcccg aggtggtcac
    19561 cgttctggcc gacaccgcga tccgcggcca taacctcgac gaggccaagg cacaggaagc
    19621 gctcaagcgg gccgaagagg cgcgcgccac cgcgcaggac aaggtcgaaa tcgccgcggt
    19681 ggaagcggaa ctggcgatgc tcgcggcaca actggccgct attcgccgtc tgcgcaacag
    19741 aaagtaaacg cacgcctttc gtcacgatca aagcggcctt ccggccgctt ttttcatttg
    19801 catcaccgtc gtgcagcgcg cgccgtcacg ggcgcgtcga ttgctacccc ctagtcgagc
    19861 gggcactgcg tttacaccga cgatgcggct gacgcacaat gggcgcgagg gcgttcgggt
    19921 ttgcacccac cgctacatcc tttgcgtcat cagcgagtga gtacgatgcc atcgtcgcaa
    19981 cagcaagcgt tcatgccgat gtccgatgcg ccgcatcagc tcagcgagca cgacgtctat
    20041 ctgttcaggg agggcacgca cgcgcagctg tacgaaaagc tgggctgccg cttgcgtgac
    20101 gaggtcgcgc acttcggcgt ctgggccccg aacgcacagc gtgttgccct catcggcgag
    20161 ttcaacggct gggacccgga tgcgcatcct ctgcgtgcgc gcgccgatca cagcggcatc
    20221 tgggaaaccg atgtccccgg ggtacagccg ggacagacct acaaattccg catctggtcg
    20281 cgccatggcg ggtacgtggt cgacaaggcc gaccctttcg cgttttatgc ggaagtggcg
    20341 ccggccacgg cttcgcgcgc ctggcgcatg gattacgagt ggggcgacgg cgaatggatg
    20401 cgacagcggc gcgaacgcaa tgcgctcgat cgcccgatct cgatttacga agtccatctg
    20461 ggctcgtggc ggcgggacgg tcaaggcaac ctcctcggct atcgcgacat cgcgcatcaa
    20521 ctcgccgagt atgtgcagcg gctcggcttc acccatgtgg aactgatgcc ggtcacggaa
    20581 caccccttct acggctcctg gggctatcag accaccggct acttcgcgcc gacctcgcgc
    20641 tacggcaatc cgcaggactt catgtacttc gtcgatgtcc tgcatcgcca cggcatcggc
    20701 gtcattctcg actgggtgcc ttcgcacttc cccggcgatg ctcacggcct ggcttatttc
    20761 gacggcacgc atctgtacga acacgccgat ccccgccagg gtttccatcc cgaatggaat
    20821 tcgtacatct tcaactacgg ccgcaacgaa gtgcgcgcct tcctgctgtc cagcgcgcgc
    20881 ttctggctcg accgctatca catcgacggc ctgcgggtgg atgcagtcgc atcgatgctg
    20941 tacctggact acggccgcaa gaaaggcgag tggataccga accactacgg cggcaaggag
    21001 aatctcgagg ccatccattt cctgcgcacg ctcaacgaaa tggtgtaccg cgattttccc
    21061 gatacgcaga cgatcgcgga ggaatcgacc gcctggccga tggtgtcgcg gcccaattac
    21121 attggtggtc tgggtttcgg catgaagtgg aacatggggt ggatgcacga caccctgcac
    21181 tacatgcagg aggacccgat ccatcgcaaa taccatcatc accagatgac cttctcgctg
    21241 atctatgcct tcaacgagaa ttttgtactg cccttctccc acgacgaagt ggtgtacggc
    21301 aagcgttcct tgctgaacaa gatgccgggc gacctgtggc agcagttcgc caatctgcgc
    21361 gccctgtacg gctacatgtg gggccacccg ggcaagaagc tcctgttcat gggcggcgag
    21421 ttcggccagc gcagcgaatg gaatcacgat gccgcactcc aatgggatct gctccaatac
    21481 ccggagcacg aaggcctgca gcgctggttg agcgatctca acctcgcgca ccagcgcgaa
    21541 ccggcgctgc accaggtcga tttcaacgat cagggtttcg agtggatcga cgccaacgac
    21601 agcgagacca gcgtctacag cttcatccgc aagccgcgtg acgccggaca gccgattgcg
    21661 gtggtctgca acttcacgcc cttgccgcgc gagaactaca tcatcggcgt gccgcatcgc
    21721 ggctattggc acgaaatcct gaacagcgat gcgtcgctct acggcggcag cggcatgggg
    21781 aactacggcg gtttgcagac ttcgccggtg ccggcgcatg ggcgcctgca ctcgttgacg
    21841 attaccctgc cgccgctggc cactgtgtac ttccgctatg aaggagcggc ggcctagctg
    21901 gcgcagccat cgagatcgac agcggagctg ccgcgcgcaa gacgcgcgca gcgttcgatg
    21961 aaggaggagg caccttgcaa gcgaacgcgc aaacccttcc gccgcacgac gggcgcgcac
    22021 gcgcggtcat cgaatcgatt acgccgcagg tcgacaacgg tcgcttcccg gccaagcggg
    22081 tgatcggcga tctggtggaa gtgcaggccg actgcttcgc cgacggtcat gatgcgatcg
    22141 cggtcgtgct gaactggcgc cgcgccggcg agcccgaatg gaagcaggtg gcgatggcgc
    22201 cgctgggcaa cgaccgctgg accggctgct tcgtcgtcga tgccctcggt ttgtacgagt
    22261 atcgcgtcat cgcctgggtc gatcacttcc tgtcctggcg gcgggatctt gcgcgccgcg
    22321 aagatgcgga ggacattcgc gtcgccgcac tggtcggcgc gcaactggcc gaggaagccg
    22381 cgcaacgcgc ggatgccggc gatcgcgacc tgctgcgcga ctgggctgca cgattgcgcc
    22441 aaagcggcgc gccgcaggcg ctcaaggcgc tgagcctcga cgagactttc gccgcgctgg
    22501 cgatgcgcca tccggatcgc cgctacgcct gcagcagcga tccgctgcaa ctgcgcgtcg
    22561 accctgtcca tgcgcgcttc ggaagctggt atgaactttt tccgcgttcg gccgccgacg
    22621 agccgggccg acacggcacg ctgcgcgacg tcgaggcgcg cctgccctat gtcgcggcca
    22681 tgggtttcga cattctctat ctgccgcccg tccaccccat cggacggatc aaccgcaagg
    22741 gtcgcaacaa cgcgctggtg cccgaacccg acgatgtcgg cagtccctgg gccatcggcg
    22801 ccgacgaagg cgggcacaaa tcgatccatc ccgacctggg cacactggag gatttccgca
    22861 gcgtcgtcgc gagcgcgcgc aagcaaaaga tccgcatcgc gctggacatc gcctttcaat
    22921 gcgcgccgga tcatccctat gtcaaggcgc acccggagtg gttcaaacat cgcccggacg
    22981 gcagcgtgca atacgcggaa aacccgccga agaagtatca ggacatctac cccttcgatt
    23041 tcgaatcgga cgactggcag ggcctgtgga ccgaactgct gagtattttc cgcttctgga
    23101 tcgatcaggg cgtcacggtg ttccgcgtcg acaacccgca taccaaggct tttccgttct
    23161 gggagtggtg tatcggcgaa atcaaacgca cgcatccgga aacggtcttt ctgtccgaag
    23221 ccttcacgcg tccgcgggtg atgcatcgcc tggcgaagct gggcttcacg cagtcgtaca
    23281 cctacttcac ctggcgcaac accaagcagg aactgattga ctacttcaat gagttggcgc
    23341 acgggccggg cgcggattat ttccgtccga atgtgtggcc caacacgccc gacatcctgc
    23401 ccgagcatct gcagtacggc ggacgtcctg cgtttatggc gcgcctagtg cttgccgcaa
    23461 cgctggcgag cgcctacggc atctacggcc cggccttcga acacatggag caccggccgc
    23521 gcgagccggg cagcgaggaa tatttggact cggaaaagta tcagctacgc cactgggacc
    23581 tcggccgcga cgacagtctg gcggagttca ttgcgcgcgt gaatgcaatc cggcacgaga
    23641 acgtcgccct gcaggacaac cgcagcctgc gcttcctgga aatcgacaac atcaacctgc
    23701 tcgcctacgc caagtcgggc gtcaacggtg acagcctgat cgtcaccgtg gtcaatctgg
    23761 attaccacca cacgcaatcg ggctggatcg agctggattt gcaggccttc ggcatggacc
    23821 cggatgcttc ctaccagatg cacgaactgc tgaccggcgc gcgttacctc tggcacggcg
    23881 cgcgcaacta cgtgagcctg gacccgcaac gcagccctgc gcaaattttt cgcgtgcgtc
    23941 gccgcgtgcg gcgcgaacag gatttcgact acttcctttg agtaaggcgt ggatcagacc
    24001 gaaccgaacc tgctgcagca accgcatccc gccgccgaga cggacgacgg cctctggtac
    24061 aaggacgcgg tcatttatga actgcacgtc aaggcgttct acgacagcaa taacgacggc
    24121 atcggcgact tccgcggact gacggcgcgg ctggactaca ttcaggaact gggcgtcaac
    24181 accatctggc tgctgccctt ctatccttcg cccatgcggg acgacggcta cgatgtggcc
    24241 gactaccaca acgtccatcc agactacggc acgcgccagg acttccgcgg cttcgtgcga
    24301 gaagcgcatc ggcgcggcct gcgcgtcatc accgaactga tcgtcaacca cacttccgat
    24361 cagcacccct ggttccaggc ggcgcggcgc gcacccaaag gctcgagcaa acgcaacttc
    24421 tacgtctgga cggacgaccc gaacaagtac gccggcacgc gcatcatctt caccgatacc
    24481 gaaacctcca actgggcgtg ggacgaggtg gcgcagcaac actactggca ccgctttttc
    24541 agccatcagc ccgatctcaa cttcgacaac ccgaacgtgc tgaaggcggt gatccgcacc
    24601 atgcgcttct ggctcgacat gggcgtggac ggcttccgtc tggacgccat tccttatctg
    24661 gtggaacgcg agggcaccaa caacgaaaac cttccggaaa cgcatgcggt gatcaaagaa
    24721 atccgcgctg cgctcgacgc gcactacaag gacaagctgc tactggccga agcgaaccaa
    24781 tggccggagg acgtgcgcga gtattttggc gacggcgacg aatgtcacat ggcctatcac
    24841 ttcccgctca tgccgcggct gttcctcgcc atcgcgcagg aagaccgcta tccggtcatc
    24901 gaaatcatgc ggcagacacc ggacattccg gacaactgcc agtgggcgat cttcctgcgc
    24961 aatcacgacg agttgacgct cgaaatggtg acggatcgcg agcgcgacta catgtacgaa
    25021 atcttcgcca ccgatccgcg catgcgcctc aacgtcggca tccgccggcg gttggcgccg
    25081 ctgatggaca acgaccgcgc ccgcatcgag ctgatgtcct ttctgctgtt caccctgccg
    25141 ggcacgccgg tcgtctatta cggcgacgag atcggcatgg gggacaacat ctatctgggc
    25201 gatcgcaacg gcgtgcgcac gcccatgcag tggagcttcg accgcaacgc cggcttttcc
    25261 cgcgcggatc cgcagcgcct ctatctgcca cccatcatgg atccggtgta cggcttccag
    25321 gcggtgaacg tggaagcgca ggcacgcaac ccttcttcgc tgctcaactg gacgcggcgc
    25381 atgatcgcca tgcgcaaggg tttcaaggct ttcggccgcg gcacgctcac cttcctcgaa
    25441 ccgggcaacc gcaaagtgct cgcctatcta cgcgagtacc gcgacgagac catcctgtgc
    25501 gtcaccaatc tttcgcgtta tccgcaggcc gcggaactgg aactgtcgcg gttcgagaat
    25561 cgcgtgccgg tggaaatcct gggacgcgtg ccctttccgc ccatcggcaa actgccctat
    25621 ttgctgacgc tgcccggcta cggctattac gtcttccgcc tggccaccga tgcggagccg
    25681 ccggcatggc atgcgcagcg gctggtgcac gacgatctgc ccacgctggt gctgctggaa
    25741 ggctggcgca ccttcttcat gggcagcggc ggtgcgagcg acgtgcgccg cgccattgcc
    25801 tcgcgcagcc gcgaacagtt gaagcgcgaa gtgctgctgc cgcatctgca gacccgccgc
    25861 tggttcgccg ccaagggcca ggaagtcacc gacatcagca tcatcgagga aggcgaatgg
    25921 cgcgcggacg acggcggcgc ctggctgttc actttcgcgc gcgtggagct ggccgatgcg
    25981 ccggcgcaag tctatttcct gccgctgggc atcgcctggg aagagggcga ttacgatccg
    26041 gcagcggagt acggcagtgc cgcgctcgcg cgggtgcggc agaaggccaa gaccggcatc
    26101 ctccacagcg ccttcggcga tccggcgttc tgtcgcgcgc tcgcgcgcgc catgggcgaa
    26161 aaccgcgaag tgtccttcgg acacgggcgg ttgtacttct cctcgacgcc ggtgtatgcg
    26221 cagatcgccg acggcttcga cgaggaagtg cggcagccgc cgaccgagca gaccaatacc
    26281 ggcgtgttct tcggcacgcg tgcgtacctc aagggctatc gccggctgca ggcgggcacc
    26341 aatcccgagc tggagatcgg ccgcttcctc accgtcagct cgcccttccc gcacactgcg
    26401 ccggtggccg gcgcgttgga atatgtggcg gccgacggcg agcgcgtcac gctcgcgctg
    26461 ctgcagccct atgtggagaa ccagggcaac ctgtgggact actcgctcga ttatctggag
    26521 cgttacctgg cgtccttcgg cgtgcagccg gaaccggtgg acgatcacgc ggacgtcaac
    26581 ctccatgcct tcttcctcgc ccaggcgacg cgcctcggcc atcggctcgg ctcgctgcac
    26641 gaggccttcg cccggaccac gggcgagccc gacttcgatc ccgagccggt tacgccggcc
    26701 gacatcgaac aatggcgcga cagcgtgcgc gatgaactga tcctcacttt cgacctgctc
    26761 gaacgccgcc gcgcgagcct gcccgagtcg atgcgtcacc tggccgatca gctgctggcg
    26821 ctgcgaatgc gactggtcga gcggctcgat gcgctgcaac tgccgatcga cgggctggtc
    26881 aagacgcgct accacggcga tctgcacttc gggcaggtgc tcatcgcgga gaaggactat
    26941 ctcatcgtcg atttcgaagg cgagccgagc cggccgctgt ccgaacgccg acgcaagcat
    27001 tcgccgttgc gcgacgtggc cggcatggtg cgctcggcgg actatgccgc acgcacggtc
    27061 ctgacccgcc tgggtgccgg agcgatggag cgtcataccg ccatcagcga ggccgcacgc
    27121 gagtggcggc gcgcggtgag cgaagcgttc atcggcggct atatgcgcgc cacgcggcac
    27181 gttccttccg tgccgcacga accggccgcg ctgctcgcgc tgctggagtt gttcattatc
    27241 gagaaggcct tgtacgaagt tcgctacgag atggaccatc ggccggactg gctcgacgtg
    27301 cccatcggcg gattgctgga gattgcgagt ccggctgccg aagactgagc ggccggtatg
    27361 ccggccgcgt aaaggaggga agcgatgatc acccgcaacg aaaatgtcct ggccatggtg
    27421 ctggcgggcg gcgagggcac ccggctgtcg ccgttgacca tcccccgctc caagcccgcc
    27481 gtgccattcg gcggccgcta tcggatcgtc gatttcgtac tgtcgaacct ggtcaactcc
    27541 ggcatttact cgatctacgt gctggtgcag tacaaatctc agtcgctgat cgaacacatc
    27601 cgcaacggct gggtcatggc gccgatcttt ccgcgccagt tcgtcaccgt cgtaccgccg
    27661 cagatgcgcg aagggccgga gtggtttcag ggcaccgcgg atgcgatcta ccagaacatc
    27721 aatctggtgc aggacgcgcg cgccacgctg gtcgcggtgt tcggtgccga tcacatctac
    27781 cgcatggacc tcacccagat gatcgtggcg caccgggagc gcaaggccga tgtgagcatc
    27841 gccgccgtgc cggtgccacg cgacaaggcg cagggtttcg gcatcatggg tgtggccgac
    27901 gacggccgca tcgtcgactg ggtggaaaag ccgcaggacc cgccggaaat gcccggacgt
    27961 ccgggctggg cgcttgcttc caccgggaac tatctgttcg acgccgattt gctcgtcggg
    28021 gcgctgaccg ccgcgaaaaa gcgcggcgag aaggatttcg gcaaggacgt cattccggcg
    28081 ctcatgaaaa cgcatcgcgt ctatgcctac gatttctcca agaaccgcgt tcccggcgtg
    28141 cgtcagtacg aggaacaaag ctattggcgc gacgtgggca cgctcgatgc ctattacgac
    28201 gcgaatatgg atacgctggg tccgcagccg cgcatccagt tgttcaaccg gcagtggccg
    28261 ctgcgctcca gtaactatca ggggccgccc gccagcctgt tcgacactca cgtgcagaac
    28321 agccacatcg gcgcgggcgg gcgtttcggg cgctgcaccc tacgcaactc cgtcatccgt
    28381 cgcgaagtga gcatcggcga cggcgccgtc gtggaggatt cgatcatcat ggactactgc
    28441 gtcatcgccg aaggtgcgca catccggcgg gcgatcatcg atcgccacaa catcatcggc
    28501 gccaacgaac gcatcggctt cgaccgcgag gcggatgccg cgcgctttca cgtgaccgaa
    28561 tccggtctgg tcgtggtgcc catggccgaa atcatgtccc gcgccgacgc gtacgaatga
    28621 gcacgcgcct cgccgttcag ccgattaccg ccgtctggcc cggcaagccc tatccgccag
    28681 gcgcgacctg ggacggggaa ggcgtgaact tcgcgctgtt ctccgagcat tcggagaagg
    28741 tggaactgtg cttcttcgat gcacagggtc ggcgcgaagt acagcgcatc gaattgcgcg
    28801 agcgcaccga ccagatgtgg cattgctacc tgcccgaagc gcggccgggc atgctttacg
    28861 gctaccgcgt gtacggcccc tacaagccgg aggaaggaca ccgcttcaac ccgaacaagc
    28921 tgctgctcga cccttacgcg atgaacatcg tcggcaacat gcgctggagc gatgcgctgt
    28981 tcggctatca gatcggcaac aagcgcgagg acctgtcctt cgaccgccgc gacagcgcga
    29041 gcggcatgcc caagtgcaag gtcatcgacc cggccttcac ctggggcgac gaccggcgcc
    29101 cgaacgtgcc ctggcacgac atggtgatct acgaaatgca cgtgcgcgga ttcaccatgc
    29161 agcatccgga tgtgccgccg ccgctgcgcg gcacgtacgc cggtcttgcc accgccccgg
    29221 tggtcgaata tctcaagcgc ctgggcgtca ccaccgtgga attgatgccg gtgcacacgt
    29281 tcgtcgacga ccgctatctg gtgcagaagg ggctatcgaa ctactggggc tacaacacca
    29341 tcggttttct cgcgccggaa gcgcgctact cggccagcgg caaggtggcc gaattcaaga
    29401 ccatggtgaa gacgctgcac tcggcaggca tcgaagtgat cctcgacgtg gtgtacaacc
    29461 acacggccga gggcaatcac ctggggccga cgctgtcgtt ccgcggtatc gacaatgcat
    29521 cctactaccg cctcacggcc gagaatctgc gctactacaa cgactacacc gggtgcggca
    29581 acacgctcga catgcagcac ccgcgctcgc tgcaactgat catggacagc ctgcgctact
    29641 gggtgacgca gatgcatgtc gatggcttcc gtttcgatct ggcttcggcg ctggcgcgcg
    29701 aactgcacga ggtggatcgc ctcggcgcct tcttcgacat catccgccag gacccggtgc
    29761 tgtcgcaggt caagctcatc gcggaaccct gggatctggg cgagggcggc taccaggtcg
    29821 gaaactttcc catcggctgg gcggagtgga acgacaaata ccgcgacacc atgcgcgcct
    29881 attggaaagg cgacggcggc ctcatcgccg aattcgccac gcgcctcacc ggatcgagcg
    29941 atctgtatgg ccggggcggc cggcggccct attccagcat caacttcatc gccgcgcacg
    30001 acggcttcac gctacacgac ctcgtgtcct acaacgacaa gcacaacgag gccaacggcg
    30061 aagacaaccg cgacggtcac gacaacaatc tgtcgtggaa tcacggtgtg gaagggccca
    30121 ccgacgatcc gcagatcaac gaactgcgcg aacggcagaa gcgcaacatg ctcgccacgc
    30181 tgttcctctc gcagggcgtg cccatgctgc tcgcgggcga cgaactgtgc cgtacccagg
    30241 gcggcaacaa caacgcatac tgtcaggaca acgaaatcag ctggcaggac tggacggcga
    30301 cgccagagcg cgcgcagtta tacgaattca cgcggcgcat gatcgccatc cgccatgcgc
    30361 atccgctgct gcgccgccgc gacttcttca ccggcgacgc gaccaacggc catacgggca
    30421 aggacatcgt ctggctcaag cctgacggta ccgaaatgac cagcgaggag tggcatcagc
    30481 agttcgcgcg ctgtctgggc gtctatctct cggggcagtc gatgaccgaa acggacgagc
    30541 gcggccggct gctggaagac gacgacttca tcatcctctt caatgcgcat cacgaagagt
    30601 tgccgttcgt cattccgcat ttcgaggcac cgtcgcgatg gcgcgtcttg atcgactcga
    30661 gccagcccga cagcggtgaa tggaccgcct tcgaaagcgg cgcccagtat ccgttgcatg
    30721 gccgttcgct tgcgctgctc atccgcccgc ggcgccgcag cgtcgtgcca tgaacgcaca
    30781 ggccgcactg tgaagcgacg acacctcatg cccttcggca cgcgactgct cgacgacggc
    30841 ggcacgcgct tcaggctttg ggcgccgagc gccgaacggg tcgatctggt gctggcgatg
    30901 gacagcggcc gcgcggaact gcccatgcgc gccgttgaag acggctggta cgaaaaagtc
    30961 gttcccggca ccggcccggg ggcgcgctat gcttaccgca tcaacggcgg catctccgtc
    31021 cccgatcccg cgtcgcgctt caatccggaa gacgtccacg cagccagtga agtcatcgat
    31081 ccgcacgcct acacctgggg cgacgccgac tggcgcggcc ggccgtggga agaggcggtg
    31141 ctgtacgaac tgcatgtggg cacgttcacg ccggcgggca cctatgccgg agcgatagag
    31201 aagctcgacc acctcgccga tctgggcgtg acggcgatcc aactgatgcc gctcgccgag
    31261 tttccgggca agcgcaactg gggctacgac ggcgtgctgc tgtacgcgcc caatgccaac
    31321 tacggccgac cccaggattt gaaacgcttc atcgatgcgg cgcatgcgca cgggctgatg
    31381 gtgctgctgg atgtggtgta caaccacttc ggccccgaag gaaattatct gcacgcctac
    31441 gcggacgcct ttttcaatcc gcgccatcag acgccctggg gcgatgcgat caatttcgac
    31501 gggcccggcg cgcatacggt gcgcgacttt ttcattcata acgcgctgta ctggatcgag
    31561 gaatatcact tcgacgggct gcgtctggac gccgtgcacg ccatcgccga cgattcgcag
    31621 ccgcacatcc tgcgcgaaat ggcggaagcg gtgcgtaccg gccccggaca cgatcgtcac
    31681 gtccatctgg tgctggagaa cgagcagaac caggcgagtc tgctcggtcg cggtgcggaa
    31741 cacgaaccgc tgatcgccac ggcgcaatgg aacgacgact gccatcacgc ctttcatgtg
    31801 ctgctcacgg gcgagcgcga cggctactac caggactacg ccgacaaacc gctcgcccat
    31861 ctgggacgct gtctggccga aggcttcgcg ttccagaacg acgtctcgcg ttatcgtcgc
    31921 ggggcgtcgc gcggggaacc gagcgtgcat ctgccggcca ccgcctttgt cgatttcatc
    31981 cagaatcacg atcagatcgg caaccgcgcc ttcggcgaac gcatcaaccg cctcgcaccg
    32041 cccgaggcgg tggaggctgc cgccgcgtgt tatctgcttg cgccgagtgt cccgatgatg
    32101 ttcatgggtg aggagtacga tgcttccacg ccttttctct tcttctgcga cttcgggccc
    32161 gatttgagcg ccagcgtgac cgccggccgc cgcagtgagt tcggtcgctt cgaacgcttc
    32221 aaggacccgg ccgtgcaggc gcagattccc gatccggtgg acgccgaaac ctacgaacgc
    32281 tccaagctgc gctgggagga agtaaacgaa ccggcgcatg cgcagcgcct gaaactttat
    32341 cggaaattgc tcgcgctgcg acgcgcgcac atcgtgccgc gcctgcacgg catgccaccg
    32401 agcggcgcct atgtcgcgca cgaacgcgcc ggactgctgg tgcactggac gctcggcgac
    32461 ggcacgcggc tgcagctcaa tgcgaacctc gccacgcagc cggccgagga ggtgagcgcg
    32521 ccgctgccgg atacgcttta cgccagtccc gctgtggagg acgcgcacct gggcgacggc
    32581 cgtctgccgc cctggtcggt ggtctggtcg atgcgggctg cgtgaggaag ggcgacgatg
    32641 cgtgaagcgc tgacgcgcct ggcccgactg cacggcatcg ccccgaacta ccacgacatc
    32701 tggggcaccc atcatcaggt gagcgacgaa tcgctcgtcg cgctgcttgg cgccatcggc
    32761 gtgcgcgccg atacaccgga ggcggtcgat gtcgcgctgc aggaggcgac gcacgaacgt
    32821 tggacacaca tcgtcccgcc ggttctcgtg ctgcgcgccg gggcgcaacc ctggcgtatc
    32881 gcctgcaatg tcctcgtgga acgactcgat gcgcccgtac gctggacgct gcaactggaa
    32941 acgggcgaag tgcgggaagg ccggttcacg ccctcgcatt gcgaggaaga aactcatgca
    33001 cacaccccgg acggccacta cagccgccgt ctgtggcaac tggaagtgga cgcgccgctc
    33061 ggctatcacc gcctcagtat gctgcgcgac gaggagacgc tggatacctg cgcgttcatc
    33121 gtcgcgccgc agacctgtta tgcgcccgaa gccctgcagg aagacggtcg cgtgtggggc
    33181 gcggccgtgc agttgtacgg cgtgcgttcg gagcgcaact ggggcatggg tgatttcggc
    33241 gatctgcaga cgctgatcga ggagtgggga caacgcggcg cgggcatcat cggtctcaat
    33301 ccgctgcatg cgctgtttcc gcacaacccg tggcacgaca gtccctacag cccgtccagc
    33361 cggctgtaca agaacgtgct gtatctggat gtcgaagcga tcgaggactt ccgcgaatgc
    33421 gaagcgacgc gcgcgctggt atacggcgcg gcatttcagg cgcgcctcaa tgccttgagg
    33481 gatgctgaac aggtggatta tcccggcgtc gccgcggcca agttcgagat cctcgaacgc
    33541 ctctacgccg gcttttgcga acgccatctt gcgcacggga cggatcgcgc cgccgccttc
    33601 gccgaattcc gccgtcgcgg cggcgaatcg ctgcggcgcc atgtgctgtt cgaagcgctg
    33661 caggaacatt ttcaccgcca caatccggat gtctggggct ggccggtctg gcccgaagcc
    33721 tatcgtcatc cggacctgcc ggccgtcgct cagttcgagc gcgaagcgca agcgcgcatc
    33781 ggcttctacg cttggctgca atggcaggcc gatctgcaac tggcgggggc aggcaagcgt
    33841 gcccacgacc tcggcctggg cgtgggggtg tacgaggacc tcgccgtgtc catcaatcgc
    33901 ggcggcgccg aggcgtgggc gaaccagcat ctctacgcgc tcaccgcgag cgtgggcgca
    33961 ccgcccgacg cgctcggccc caagggacag aactggggat tgccgccgct cattccggaa
    34021 cgcctgcagg ccgaggccta tcggccgttc atcgccacgc tgcgcgcgac catgcgacat
    34081 gccggcgcgg tgcgcatcga tcatgtgatg ggcttgtcgc gcctgttctg ggtgccgccc
    34141 ggccgcgaag cgaccgacgg cgcctacatg cactacccct tcgacgaact gctgggcgtg
    34201 ctggcgctgg aaagccatcg caaccgctgt ctggtcatcg gcgaggacct gggcaccgtg
    34261 tcggacgaag tgcgcgaggc gctggcgcgt catcgcgtcc tgtcctatcg actgctgtac
    34321 ttcgagcgcc gcgacggcgg cgacttcaag tcgccgggcg agtatccgcg cgaggcgatc
    34381 gtgtcggtgg gtacgcacga tctgccgacg ctcaccggct ggtgggaagg acgggatatc
    34441 gcgctgcgta ccgaactcga tctctttccg gacgaggcca cgcgcgagcg tcagcatgtg
    34501 gaacgctcgc aggatcgcgc acggctgctg ctcgcgctgg agcgcgaagg cctgctgccc
    34561 gaaggcgtga cgcccgatcc gtcttcggcg ccgcagatga cgccggagct ggtgcgcgcc
    34621 gtatcggcct atctcgccga cacgccgtcg cgggtgctgg tgctgcaact ggaggacgtg
    34681 ctgtgcgtgc gcgaccaggc gaatctgccg ggtacgacgg acgaacatcc caactggaaa
    34741 cgcaagctgc cgctcacgct ggagcgcatg cgcgacgatc cgcgtttcat cgagttgtgt
    34801 cgaactgtcg gcacgaagcg ggagagtcat cgtccaaagc cccgcgcgcg gagtgagatc
    34861 gaagccgtca ttccgcgcgc cacctaccgg ctgcaactgc atcgcgaatt caacttcgac
    34921 gacgcgacgc gcatcctgct ttatctaaac gagttgggcg tcagccatgt ctattgctcg
    34981 ccctatctgc gcgcccggtc ggggagcacg cacggctacg acatcatcga ccacaacacg
    35041 ctcaatccgg agatcggcga cgaggccgcg ttcgagcgct tcgtcacggc gctcgcgcgc
    35101 cacggcatgg gacaaatcct cgacatggtg cccaatcaca tgggcatcct cggcgcggac
    35161 aacgcctggt ggctggacgt gcttgaaaac ggccaggctt cgctgtacgc ggactacttc
    35221 gatatcgact ggcactccct cgatcccgcg ctgcgcggca aggtactggt gccggtgctg
    35281 ggtgatcact acggcatcgt gctggagcgc ggcgagttgc ggctggcttt cgatgccggg
    35341 gccggcgcgt tcgtggtcca gtactgggac catcgctttc ccatcgaccc gcaacagtat
    35401 ccggcgctgc tgcatgcggc cgcgcaaaat ctcgcacccc atgcgctgtc ggcagaggcg
    35461 cacgccgaat ggcagaccct ggccaccgcc ttcggtcatc tgcccgcgcg cgacacgcag
    35521 gatgcggcgg cgcttgccga acgccagcgc gacaaggacg tgcacaagtc gcggctggcc
    35581 gaactggtgc aaagccatgc gttgctgatg gaggcggtcg aacgcgtggt gcggcagtac
    35641 aacggcacgc cgggcgagcg cgcaagcttc gatgcgctcg acgcgctgct cgaagcgcag
    35701 gcctaccgtc ttgcctactg gcgcgtcgcc acggacgaga tcaactaccg gcgctttttc
    35761 gatatcaacg atctggccgc gctgcggatg gagaacccgg cgaccttcga agcgacgcat
    35821 cgcttcgtcc tcgaactgtg cgcggcgggc aaggtcgacg ggctgcgcat cgatcacccg
    35881 gacggtctct acgatccggc cgaatacttc cgtcgcctgc aaacgcgcta tgcgcaactg
    35941 gccggcgcgg aagcggagag tgcgccggcg cgcaagccgc tctatctggt gatcgaaaaa
    36001 atcaccgcgc cgcatgaaga tcttccgcag gattggccgg tgcacggcag caccggctat
    36061 cgctacgcga atgtgctgaa cggcgtgttc gtcgatacga cggcgcaaag cgcgatgacg
    36121 cgtgcgtggt cggccttcgt cggcgaggac gccgtacctt tcgaggaaag cgcctatcgc
    36181 ggcaagcggc tgatcatgca gaccgcgctg gcggcggaac tcaccgtgct gtcgaaccgt
    36241 ctgttgcgca tcgcgcgcaa ggaccggcgc acgcgcgact tcacgctcaa cacgctgcgc
    36301 caggcgctga ccgaagtggt ggcctgcttc ccggtctatc gcacttacat cgtcgagagc
    36361 gcgagcacgc aggaccagcg ctatgtggat tgggccgtgt cgcaggcgaa gcggcgcatt
    36421 cccgccgccg aggacagcat tttcgaattc atccgcagcg tgttgctgct gcaaccgccg
    36481 gaggatgccg acgaggcgct gatcgagcgg tatcgcgcga ccgcgatgcg ctttcagcag
    36541 tacaccgcgc cggtcacggc gaagggcgtg gaggacacgg ccttctatcg ctacaaccgg
    36601 ctggtatcgc tcaacgaagt gggcggcgat ccgcaccagt tcggcatgac ggtgaaggcc
    36661 tttcacggtg cgagcgcggc gcgcgcgcaa cgctggccgc acaccatgct ggcttcctcc
    36721 acccacgaca acaagcgttc ggaggatgtg cgcgcacgcc tcaacgtact gtccgagatg
    36781 ccggccaact ggcgcctcgc gctgcgtcgc tggagccgca tcaaccgcgc caagcgcggc
    36841 cgcatggacg gacagccggt gccgtcgcgc aacgacgagt acctgcttta ccagatcctg
    36901 ctcggcagct ttccggccgg gacgatggat gccgatgggc tggctgtcta tcgcgaacgc
    36961 atcgagcgct acatgaccaa ggcgatgcgc gaggccaagc gccataccag ctgggtgaac
    37021 gtcaacgagg agtacgagcg cggcgtgacg gcgttcgtgc aggcgctgct gggcaggccg
    37081 gacgacaatc tgtttctcga cgactttctc gcgttggcgc gtccgctggc ctggtacggc
    37141 gcgttgaaca gcgtggccca gacgctggtg aagctttgtt cgcccggcgt gccggacatc
    37201 tatcagggca acgagacact cgactacagc ctggtcgatc ccgacaaccg ccggccggtg
    37261 gattatgacc atcgtgcacg gctgctgcgc gacgcacaga cgatggccgg acggcaggac
    37321 ctcgcgacgg ccgcgcgcga actgctgcaa ccggaacaca tccacgacgg ccgcgcaaaa
    37381 ttctgggtcg tctggcgtgc gctgcaattg cgacgggaat gggaaacgct gttccggcag
    37441 agtgactatc tgccggtgca ggcgcagggc gagcgggccg cgcatctgct cgcctttgcg
    37501 cgccggcacg aggaggcggg cgtcatctgc gtggtcggcc ggctgtatgc ctcgctgggg
    37561 ctcgaggtcg gcgagccgcc cctgggcgaa cgcgcgtggg gcgatacggc cctcgacctg
    37621 agcttcctgc cgccgcagac ccgcttgacc aatgtgctca ccggcgagat gctgcggtgt
    37681 cgcgacggac agttgccggt cgcctcgctt tttgcaaatt tcagcgccgc gctgcttcgc
    37741 tatggcgaag cctgagcgcg acctcgtcag ccatgttgta tttctccacg cctgtcgaag
    37801 cgcaactggt cgagcgctgc cgcaaacgct ccatcgaact gctgaagcga aacctgtcgc
    37861 cggccggaat actcgccgcc acgccgggcg agcgtgccga agcgcgcagc tacaccgcca
    37921 tcttcgcccg tgacgccgcc atctgcgcgc tgggcatggc gctgtcgggc gaccccgcgc
    37981 tggagcgcga agcgtccacc ggcatgctca cgctcgccgc gcatcaggcc gacaacggtc
    38041 agattcccaa gttcgtcgat gtgcgccagt cgacagcgga cttctggtat ctgggctgta
    38101 tcgacgccac gctgtggtgg ctgctcatca tcgcgctgct cgacgaactg cagggcagca
    38161 cgttgcgcaa gcgcctgcag gcgcaggtgc ggcaggccat cgtgtggctg gaatgccagg
    38221 agcaccagca cttccgtctg ctgcaacaga acgaggcgag cgactgggcc gacatcatgc
    38281 cgcgctcggg tttcgtgctt tacaccaatg cgctgtggta tgtggtcaag cggctgtacg
    38341 acctgccgca tgcggatgcc acgcgccgca acgccaacca gctgttctat cccttcagcg
    38401 ggcacgtggc cgaataccgg cgggcgcgcc tgctgtcgca ttattcgcgt cgcaatgcgc
    38461 gcaaccagga gctgtacctc agctttgtca acttcgcttt ctacggcgac gagggcgacg
    38521 tgttcggcaa cgtgctcgcc attctgttcg ggctggcgga cgagtcggac gcgcaccgca
    38581 tcatcaaggc gctggagcgc gcggcggtgc acgaacccta tcccgtgcgc gtggtgtgcg
    38641 atcccatcct cgaggcgagt ttcctgtggc ggccttatat gtcgcggcat cagcagaact
    38701 tcgcctggca gtatcacaac ggcggtatct ggcctttcgt cggcgccttc tgggtcgccg
    38761 cgctgtcggc caccggcttg aacgatgccg cccgccgcga actggtgaag ctcgcgcgcg
    38821 ccaacgaagt gggcgactgg gcattcaacg aatggctgca cggccggact tacgagccgg
    38881 gcggcatgga cggtcagtcg tggaatgccg gcgcgttcct gttcgcccac aaggtgctgg
    38941 agaccggcgg ccgctggttc tgattggccg tgccgtgccg tgcgctgccg cgcgcggtat
    39001 gcttggacgc ggtcttcaac ggcgcgcacc atcgcatcgc agaaggacaa gaacatggaa
    39061 tggtggtcgc aaatcgcctg gcatctcgtg ccggcgtacg tgctgctcgg tatcgtggcg
    39121 ggttttctcg cgggcctgct ggggatcggc ggcggcggaa tgatggtgcc cgtgctggta
    39181 tggctgttcg cggagcaggg ttatccgcac gaacatatcg tgcatatggg cgtggcgacg
    39241 gccatggcga cagtcatctt cacctccctg tcgagcgtgc gcgcgcatgc gcgacgcggc
    39301 agcgtgcgct gggacatcgc ccgggcgatg gcgcccggca tgctggtggg cggttttctg
    39361 ggggcgctgg tggcggaccg gttctccacc ttcgggctcg gcctgttctt cgtggtgttc
    39421 gtgtacatca tggcgaccaa catggtcgtc gaccgcaagc ccgcgcccgg gcgcagcacg
    39481 ccgggcaacg ccggcctgtt cgtggtcggt acgatgatcg gcgtcctgtc cgcgctcatc
    39541 gccatcggcg gcgcgatgat gaccgtgccc tttctcatct ggtgcgcggt gccggtcatc
    39601 caggcggtgg gcacctcttc ggcgatcggc tttccggtcg cgctggcgag cacggtgggc
    39661 tatatgtggg tgggtgccaa tgccagcacg ccggcgaccg cctggggcta tgtggatctg
    39721 cccgcgatgg cgagcatcgc aaccgccagt gtgctgctcg cgccgctggg cgcgcgtttc
    39781 gcgcatcgtc tgccgacgcg gacactgcgc cgggcgttcg ccgtgtttct gttcctgctc
    39841 gccacgcgca tgctgttcca gatctgggag tgaatccctt caaagcttga aacacggaga
    39901 gcacggagaa cacggagtaa gcctgtgctc ggctttaccc gcctttgcat tctccgtggt
    39961 tctctgtgtt tcggtctttc ggggtcaacg catcggcgcg ctcggctgcg agccatagct
    40021 gccgtgcagc ggccggctgg cgatatgacc cgtcgcatcg gagaacagga tttccacgcg
    40081 gcgattcagt tgccggcccg ccgtggtcgc gttcgtcgcc accggatagg cttcacccag
    40141 tccttgcgca tcgatgcgat cggcgctgat gccgcggtcc accagcgcct gacgcaccgc
    40201 accggcccgc cgcgccgaca actcgaggtt gtaggcatcc tgaccctgac tgtcggtgta
    40261 gccctcgatg agcacgcgcc gctgcggatc gtcgtgcaat acggcagcaa tgcggtcgag
    40321 catgcgcgat gcgccgggcc gcagacgggc gctgtccaca tcgaacatca cgtccgccat
    40381 ggtgacgatc agaccgcgct cggtttgttg cgcctgcagt tcggccagtt gcgcttgcag
    40441 acgagccgcg cgctgctcgg cgctcagagc ctgcgcctgc gcgtcgttcg cctgcatccg
    40501 cgcaagatcg agatcgcgcg tctgcgcctg tgcgagcagt tgatcgcgtt gctgactgac
    40561 gaagcggatg cgatcctccg catggccgcg tacgccgact tccgttgcgg tgagtgcgcg
    40621 ctgccgtgcg acataggcca gatgcgtcgc aagttcgctg ttgccgttgt cgcgccattc
    40681 ggcctcggcg tgctgcagcg tggtgcgtgc gcgctccagt tcgaccgggc cgtatttctg
    40741 cacgtaggga tcgtcctgtg ccgtgtgata agcctgtcgt gcgccctgca gttcgagatt
    40801 ggcggcggac ggcacggtct ggcaaccggc gcagatcagc gccgcgcccg caaggagtgt
    40861 ggcggtgcgc ttcatgatcc gcctccgcga tgtgtgcgat ccagttcggc ctgcagcgcg
    40921 cgcacgctct gctcgagttc cgccagcgca tgctgcgagc gcgtggattg cgcgcgcgcc
    40981 tgcgcaagct gcgcatccac ttccgcttcg cgtgaaagac gtatggccgc cgtgtagtcc
    41041 tgcgcttcga aagcggcttc ggcgcgcgcc agcttgtcgc gcgcggtgct gtattcgatg
    41101 gctgcgcttt gcggcgcgcc ggcctgacgc gcctgatcca gtgcggtgcg gctggcggcc
    41161 agttgagcgg tcggtgccgc gggcgtcacg gcgcagcctg caagcagcag tgccgcgccc
    41221 atcgccacgc cgccggcgca taggcggtgc ctcataacgg actccgggcg agaacgttga
    41281 tgagggaaac gctgcaaggc gcgcgcccgc gcgcgtcagc ggccgtagcg ctcgcgcagg
    41341 gcggccttct gcactttgcc catggcgttg cgcggcagtt cgtcgacgaa atgcacgcgc
    41401 ctgggcagct tgtaaccggc cagttgggtg cgcagcgcat cgacgatgcc gcgctcatcg
    41461 agcgcgcaat ccgcattctt caccacggcg gcaacgaccg cttcgccgta atccgcgtcc
    41521 ggcacgccga tgacggccga ttcgatgacg cccgggagcg cgtcgagcgc cagctcgatt
    41581 tccttgggat agacgttgta gccgccgctg atgatcagat ccttggcgcg tccgacgatg
    41641 gcgaggtagc cgtccgcatc cagctgaccg atgtcgccgg tgcggaacca gccgtcggcg
    41701 gtgaattcct cgcgcgtctt ttcgggcatg ccccagtaac cggcgaacac gttcggtccc
    41761 ttgatctgca ccatgccggg cgtgccgacg ggcagcggcg catccgtgtc gtcggcgacg
    41821 cgcagttgca cgccgggcag cggtttgcct acggtcccgc agcggcgttc gccggcataa
    41881 ggattggagg tgatcatgcc cgcttcggac atgccgtagc gttccaggat gacgtgaccg
    41941 gtccgcgcgc gccacgcttc gaaggtggcg gggagcaacg gtgccgagcc ggagacgaac
    42001 agacgcatat gctgaaccgc ttcgcgtgtc aggcccggtt cgcccagcag gcgcgtataa
    42061 aacgtcggca cgcccatcaa taccgttgca tcgggcagcg cccgcagcac cgcgcccgca
    42121 tcgaagcgcc gctgcagcag catgcgcgcg cccgacaaca gcacggtgtg gaaggcgacg
    42181 aacagaccgt ggatgtggaa gatcggcagc gcatgcagaa ggacgtcggt cgaactgaac
    42241 gcccagccgt ccgtcagagc ctgcgcgttg gagctgagat tgccgtgcgt caccatcgcg
    42301 cccttggagc ggccggtggt gcccgaggta tagatgatga ccgcaagggc gtcgtcatcg
    42361 agctcgacgt cgtcgtagcg gtcgggcgca tcgcgtgctg cgtcgatcag cgtgcccgtc
    42421 gcgtcgtcgt gcagcgtgtg cacgtgcggc gtgccggttt ccgccgccag ctccctgatc
    42481 gcatccagcg tcgacggccg gcagacgatc aggccgggct tcgcgtcctg caggaagtag
    42541 tgcagttccc cgcgctgata cgcggtgttg agcggcagat agcaaaggcc ggcacgcagg
    42601 caggcgaggt agaggaacag cgcctgcgga ctcttgtcga cctgcaccgc gacgcggtcg
    42661 ccgcgacgta gtccgaggcc ggtgagatac gcggcgaagc gggcgctttc gcgatccaga
    42721 tcggcgtagg tgtagcgttc gccgtgatcg cattcgatca cggtttccgg aagccgcagc
    42781 gcgaaggcgc gtgcgaaata agtgtaggcg ttcaaaggag actctgattc agctgtttga
    42841 tcgatgaagg cacactcgcg atcatcgcgg cactcgatcg aatccgtccc ggcgcttctc
    42901 tcccgatttc aggcgggcac gtccggatcg agctggcgtt cgatccattc gatcttgtcc
    42961 cgcagcagca gcttcttgcg cttgagccgc tgcaactgga gttgatcgca gcccggctgc
    43021 atgccgagcg tgcggatgtg ctcgtccagt tcgcggtgtt cctgccgcag caggatcagc
    43081 cgatcctgca acgaaagctc ggattcctcg tccatgctca ttcgttccgc cttgcatgca
    43141 cggcgccgcg ccgcgacgac caccacaacg ccgcgccggc aacgatcatg ggtgcggaca
    43201 accactggcc catgctcatg ttgaaggtga gcaggcccag aaaatcgtcc ggctcgcgat
    43261 agaactcggc gatgaaacgg aatacgccgt aaccgatgag aaacataccc gataccgcgc
    43321 cggtcggacg cggtctggac gaataccacc atagcagcac gaacagcgcc acgccttcca
    43381 gaccgaattg atagagctgc gaaggatggc ggggcgtggc gtccgccatg ggaaagacca
    43441 tggcccacgg cagatcgggc gatgccgcgc ggccccacag ttcgccgttg atgaaattac
    43501 cgagccgccc ggtggccagg ccgacgggga caagcggcgc gaggaaatcg gtgaggtcca
    43561 gccagcgcag ccggtacttg cgcgccaccc accacaccgc gatcaatacg ccaaggaaac
    43621 cgccgtggaa agacatgccg ccttgccaga cggcgatgat ctctgctggg tgggcgaggt
    43681 aatggcccgg tttgtagaac agcacatagc ccaggcgccc gcccagcacc acgcccagta
    43741 cggcgaagaa cagcagatcg tccagcacct gttgcgtgat cgcgccggtc cggccgcgcg
    43801 cgatgcgcca gcggccgagg aggtaggcgc cgaagaaccc caccagatac atcaggccgt
    43861 accagcgtac ggaaagcggg ccgatgctga aggcgatggg gtcgaattgt ggatgcacca
    43921 gcatcggaaa aggtcctcga cagacgcgtt gcgcgggcgg ctgccccgac ccggacgggt
    43981 atatcacacc gcctctgtcc cgggcaaaac gccggcgcgt caggcgcgct tggccatcat
    44041 cgcgcgcgcg cacgttctcg ccgcggcgat gaaggcggcg acggcgggcg agtcgtcgtt
    44101 ggcgcgccac accaggccga tttccatcag cggactgcgc tcctgcaacg ggcgatagat
    44161 tactcccgtg cgcttgaggt gcttgagtga ggcgggcacc agcgcgacgc ccatgcccgc
    44221 agcgacgagg ctgacgatgg tctgcatctg aatggcctcc tgctcgatgc gcggcgtgaa
    44281 cccggcgcgc tgacagtagc ccatcatcag atcgtagagc gggctgccaa ccttgcgcgg
    44341 gaaaagcacg aaagcctcgt cggcgagcgc agtgagcgaa agtttcgtcg gccagcgacg
    44401 cgtcgccggc agcgcggcga taagcggctc gcgataaacg gcctcgtagc gcagcgtcgg
    44461 ttcgagcggt tgcggcagca cgaagccgat gtccacttcg ccgtcgcgca gcgccgccac
    44521 ctgcacgtcc gaggtcgcct cctgcaactg cagtttgatg cgcgggtgcg cacggtgaaa
    44581 ggcgggcagc agttcgggca ggatgttgta ggcggccgtg ctgatgaagc ccagactgag
    44641 cttgccgatt tcgccgctcg cgatgtgacg cgcacccacg cgcagcgcct cggcgtcacg
    44701 cagcaggcgc cgcgcttccg gcagcagcga ggcgcccgcg tccgtcaatt gcacgccgcg
    44761 acgggtgcgc gcgaacagcg gcgtgccgag ctccgcctcc agcgcgtgaa tctgacgcga
    44821 cagcggcggc tgcgaaatgt gcagcagcgc cgccgcgcgg ctgaaattca gcgtttcggc
    44881 gacggtgacg aagtagcgca ggtggcgcag ttccaatgac gatacctgta aagtatgggc
    44941 gcccgcattt taatgtattg gacggtatgg ccgcagcggc ctagcatgcg cccattccgc
    45001 aagagcacag aggacccgat catggccgac gacaagcaaa cgcccgccaa ctggcgctcc
    45061 agactcatca cccaggggat cgcgcgctcg cccaatcgcg ccatgctgcg cgccgtgggt
    45121 ttcggcgacg gcgacttcga gaagccgatc gtcggcgtgg ccaacggcca ctccaccatg
    45181 aacccctgca acgccggtat ccaaccgctg gtcgatcgcg ccatcgcggc gctgaaggcc
    45241 gccggcgcga tgccgcagat gttcggcgtg ccgaccgtca ccgacgggat cggcatgggg
    45301 accgagggca tgaagtactc gctggtctcg cgcgaggtca tcgccgacac gatcgaaacc
    45361 tcggtgaacg ggcagtcgat ggacggcgcg ctcatcgtcg gcggttgcga caagaacatg
    45421 ccgggcggca tgatggcact ggcgcgcatg aacgtgccgg gcatctatgt ctacgccggc
    45481 accatcaaac ccggcaagtg gaagggacag gacctcacca tcgtcagccc cttcgaagcg
    45541 gtcggcgcct attcggccgg caagatgagc gaggaagatt tcatcggcat cgaaaagcac
    45601 gcctgtccga ccgtgggcgc ctgcggcggc atgtacaccg ccaacaccat gtcctcctct
    45661 ttcgaggcgt tgggtatgag cctgctcggc tcctcgcaga tggcttcgcc cgatgcggaa
    45721 aaagcggatt ccgcagcggc ttcggccgaa gtgctggtca acgccattag gaagcagatc
    45781 cgcccgcgcg acatcatcac gcgcaagtcg atcgagaacg cggtggcgct gatcatggcc
    45841 accggcggtt ccaccaatgc ggtgctgcac tacctcgcca tcgcagcggc ggcggaagtg
    45901 gaatggacca tcgacgactt cgagcgcatg cgccgcaaag tgccggtgct gtgcgatctc
    45961 aagccctcgg gcaaatacgt cgcgacggat ttccacaagg cgggtggggt gccgcaggtg
    46021 ttgaagatgc tgctggtgca cggcctgttg cacggtgact gcctcacgat caccggccgc
    46081 accattgccg aagagctggc cgatgtgccg gatgccccgc gtgcggatca ggaggtgatc
    46141 cggcagtggg acaacccgct ttacccgcac ggtcacctcg ccattctcaa gggcaacctc
    46201 gccccggaag gctgcgtggc gaaaatcacc ggtctgaaga atccgtccat caccggtccg
    46261 gcgcgggtgt tcaactccga gccggagtgc atggaagcga tcatggccaa gaagatcaag
    46321 gcgggcgacg tcatcgtcat ccgctacgaa ggacccaagg gcgggcctgg catgcaggaa
    46381 atgcttgcgc cgacctcggc gatcatcggc caggggctgg gtgagtcggt cggcatgatc
    46441 accgacggcc gattctccgg cggcacctgg ggcatggtgg tcggccacgt cgcgccggaa
    46501 gccttcgtcg gcggcaccat cgcgctggtg gaagaaggcg actccatcac catcgacgcg
    46561 cacaagcagc tcatccagct gaatgtcagc gacgaggaac tggccaagcg ccgcacggcg
    46621 tggaagcagc cgccggcgcg ctataccagg gggctgctcg ccaagtatgt gaagctggtc
    46681 tcgactgcca gcaaaggcgc gatcacggat tcgtaaagca ctgtacatgg atccagggat
    46741 atgttatgaa gcagagcgca tcactgatat ggttctgcaa cgggagagtt agtgcgcgcg
    46801 tggattaccc gaatgcacta tgcttcgaca acctaaggct tccagatcat gttgatgacc
    46861 ctgccttccg ccgctcgaga cttcgcacaa ttcatcggcg accgccgctt tggcgccatc
    46921 ctggccgacc cgccgtggca gttccaaaat cgcacaggca aagtcgcgcc ggagcacaaa
    46981 aggcttacac gctacggcac actgtctctg gaggaaatca aaggccttcc cgtcgcggat
    47041 gcggcggcag acgtcagtca tttgtatttg tgggtgccga acgcgctgct tcccgaaggt
    47101 ctgagtgtca tgcaggcgtg gggctttcag tacaagtcca acctggtgtg gcataaggtc
    47161 cgcaaggacg gtggaccgga tgggcgtggg gtgggtttct atttccgcaa tgtgacggaa
    47221 ctgattctgt ttggcgtgcg cggcaagaat gccaggacgc tcgcgccggg gcgtcggcag
    47281 gtaaacttca ttgcatcgcg caagcgtgag cattcacgca agccggacga acagtacggg
    47341 attatcgaag cttgcagccc ggggcctttt ttggaactgt ttgcccgtgg acaacgggag
    47401 ggctgggtga cttggggcga tcaggcctcg gaggattacc ggccgacctg gaacacctac
    47461 gcacacaact cgacgcacgc agccgatttg tttgtcgact agtccgcgtc cggcaagggg
    47521 tcgatccggt catcgggatc aaaggtcacc actgaagccg gaatgcctat cagcagtagc
    47581 gggcaaggat tgccaactcc gcggtcgatg cgatccatca gtttgcgcca gtgggtggtg
    47641 gcagcaccga acttgtccgc tacgaaatgt tcggcccagg cctgcgcgaa cggcaaaggg
    47701 ttcttccgcc gctccacgcg cttcatgacc gctgctttct gccttttggt cggatcgagc
    47761 ccgagcgcct ccaaatcggc gtatgcaccc acgtcatgct cgcgggcgaa tcgatggact
    47821 aattggtaga tgcgatcctg caggctcgaa ccgcgcgtaa tgatgacgcc taaactgatg
    47881 gcgccctcag cgtggaggcg cttgaagttc tctagatcgc ggtcgaaaaa cgggtctttg
    47941 ttattccact cgatttccag cgcaacgact ccgctctcga agacgcgtac atggtccact
    48001 tcgtgagagg tggattctcg ctcgataccg ttgatggttt tcttgatttc gaaatgatgc
    48061 ttgcgccagg tttgagcatg caacgcccgt cggagccgtt gtgttccctt ggcttcgccg
    48121 ccgcctgagc cgatgatctc cgtaatcgga atccgtatat cggacaatgc ccgttcgagt
    48181 tctcggacgg cttccggaaa atccacttcc agaatggctg ccgcgtgtga gacgcactct
    48241 atttcgaagc cattgttcgc cagggattcg aacatgtcct gatgcatagg atccggaaag
    48301 aagcccggat cctaggccgc ggcgcggcgc tttgtctaac gcaaaaggag gttgagcctt
    48361 ctagctggtg tgtcgaaggc ttatttaggc actgtggcaa agaacggcca agcgcgctaa
    48421 cgagcgatat cactcaggcc ggcaacagcg cctcgaccgt ctgcacgagc ttgggaatgt
    48481 cgatgggctt cggcacgtag ccgtcccaga tctgctgggt cggaacgtta tgcgaggcgg
    48541 tgatgagcac caacggaatg ccggacgtgt gctgccgcgt gcgcaacacg gcgcacagtt
    48601 cgatgccgtc catcaccggc atcatgacgt cggtgagcac gagatcgggc gcgtgctgct
    48661 cgatcatttt cagcgcttcc aggccgtgcg cggcgcaaca ggtttcgtag ccttcggcgg
    48721 acaggatgat ttccagcacc gcgcgggtgt cgtattcgtc gtcgacgatc agaacttgtt
    48781 tcatgcaagg cgcgttcagt taggtgattc acctgcacgg ggtcgcaata aacgcgcccg
    48841 tattgcggca caagcccctg taaacaagta attattcacg ccacttccgg atttgtcccg
    48901 gtcagcggtg acgaatcgct gcttgccggt tacattcatg tgctgcgccg ccgaccgaag
    48961 cggacgctca caacaccggg gggagggcag caatgcgatc gatactggtt gtgaatccga
    49021 agggcggcgc cggcaagacc acgctggccg tcaatctggc cgcgggcctc gcgtatgccg
    49081 gcgacgccgt ttgtctttgg gatctggatc ggcagcattc ggctttgcaa tggcttgcgc
    49141 gtcgcagcac acacctgccg gccatcggac ggctcgacga acgtgaacgc gcgccgcgca
    49201 aggcggactg gctggtgctc gattcgccgg ccgcgctgca cggcaagcga ctgaccgaca
    49261 tgctcaagca gtcgcacaag gtgctggtgc cgattcaacc ttcgctgttc gacatcgccg
    49321 cctcgagcga ttttctcaag gagctcgccg aggaaaaggc gctgcgcaaa aacacgtcga
    49381 tcggcgtcat cggtatgcgc gtcgatccgc gcacccgcgc cgcggcgcaa ttgctgagtt
    49441 ttttgcgcga gcatgacatg ccgctggtcg gctggctgcg cgatacgcag atgtacccca
    49501 acgccgcctt cgtcggcgaa tcgattttcg atcagccgcc ttacctcgcc gagcgcgagg
    49561 tggccatgtg ggggccgatc ctcgactggg tgcgacagcg ctgagccgag cgggggtgca
    49621 ggcatcgcgg ctgcatcaat tgcaaatcca ttgaagttca ctagaaagaa gccgtccgcc
    49681 atgcccaccg acgcacccgc aacgaaccga ctcgcacaag aaaccagtcc ttacctgcag
    49741 caacatgcca cgaatccggt ggactggtac ccctggggcg cggaggcgct gcatcgtgcg
    49801 cgggcggaac ggaaacccat cctgctgtcg atcggctatt cggcctgcca ttggtgtcat
    49861 gtcatggcgc acgaatcctt cgaggatccg gaggtcgcgg cgctgatgaa ccggctgtac
    49921 gtcaacatca aggtggaccg cgaagaacgg cccgacatcg atcagattta tcagacggcg
    49981 catcagctgt tcaaccagcg cccgggcggt tggccactga cggtgtttct gacgcccgaa
    50041 cagatgccct tctttgccgg cacctatttc cccaaggcgg cgcgttacgg cctgcccggt
    50101 ttcccgcaac tgctggagcg cgtggcggcc tactatgtcg agcacagcga ggagatccaa
    50161 cagcgcagcg gcgaggtgca ggctgcgctg gcgcgaaccc tggcggatga ggaagcggcc
    50221 gcggacgaac tctccgaact cgcgctcgac gtcgcgctgg acatgctcaa ggcgaatttc
    50281 gatgcggtga acggcggttt tggcgacgca cccaagtttc cgcatcccgt cgatatcgat
    50341 ttcgcaatgc gccgccatgc cgcaacaggc gacgagcagg cgcgagagat ggcgctgacg
    50401 acgctgcaaa agatggccga gggcggcgtc tacgatcaca tcggcggcgg tttctaccgt
    50461 tattccgtgg atgcgagctg gaccattccg cacttcgaaa agatgttgta cgacaatggg
    50521 ccgctactgc gcgtttacgc cgatgcgtgg tgcgtctcga atgacggcgc ttttgcgcgc
    50581 gtggcggaag agacggccgc ctgggtgatg ggggagatgc agtcgcccga aggcggttac
    50641 tactcttcgc tggacgcgga ttccgaaggc gaggaagggc gcttctatgt ctggtcgccc
    50701 gcggaggttg cggcgctgct gaccgacgac gaatacgacg tggtcagcaa gcactacggg
    50761 ctcgacggtc cgcccaactt cgaggataag gcctggcatc tgcgcgtggt gcagccgctc
    50821 gatgcggtgg cgaagcgcct gcaacgcgat ccggcggcat gcgaggcgac gctcgccggc
    50881 gcgcgcgcga agctgcatgc ggcgcgcgat gggcgcgcgc gccccggccg cgacgaaaag
    50941 gtgctggtca gctggaacgc gctgatgatc cagggcatgg cgggcgccgc cgcggtgttc
    51001 gatcgtcccg actggatggc ctccgcgcgc cgcgcaatgg atttcatccg ccgcgaaatg
    51061 tggcgcgacg aacgtctgct cgccacctac aaggacggcc gggcgcatct caatgcctat
    51121 ctggacgact acgcctttct gctggctgcg caactggaac tggcgcaggc cgactatcgc
    51181 gccgacgatc tgcctttcgc cgtcgcactg gccgacgcgc tgctggaaca tttcgaggat
    51241 gtggaacgcg gcggcttcca tttcaccagc cacgatcacg aagcgctgtt tcaccgtccc
    51301 aagccggcgc acgacagcgc catgccttcc ggcaacggcg tcgcaacgtt cgcgctgcag
    51361 cgtctggcgc atgtcaccgg cgacacgcgc tatctggatg cggcggagcg gacgctgcgc
    51421 gctttctatc gcaacatggc ggcgcatccc tccggctttg ccagcctgat gatcgcgctg
    51481 acggaatatc tcgagccgcc gcgtaccgtt gtgctccgcg gcgatgcaac ggccatcgcc
    51541 gcctggcgtg ccgcgcttgc gcgcgaatat cggcccagcg tcatggtgca tgcgccgtct
    51601 ggcgaggcgg gaacgctgcc gcccggactc gcgaaaccgc agccggccga gggcgcgacg
    51661 gcttatgtgt gccacggttt gtcctgtctt gcgccggttg cctcactcga atccctgcgc
    51721 gccgtactgc aaaccggaca aatacagtag catcggcctt cgtttgaccg agcaggagcc
    51781 agaagaatga aatcgatcct tcccgcgatt gtcgccggcg cgctgtgcag cgccctgtgg
    51841 agcggcgtcg ccatggccaa ccctgacgag ctgctgaagt cgagcggctg taccgcttgc
    51901 catgccgtcg acaagcgcct ggtggggccg gcctacaagg acgtcgcggc caagtacgcc
    51961 aaggagaaga acgccgaagc gttattgatg gagaaagtga agaagggcgg ttcgggcgtg
    52021 tgggggccgg tgcccatgcc gccgcaggcg catctcaagg atgagaatct gcgcaccatg
    52081 gtgaagtaca tcttgtcgct gaaatagtgc atgcaaacag cggggatcgt gtcgagcggt
    52141 cacgcacggg cgcaaattga caatgcataa acgcgctacc cataatcggc cgccccaggc
    52201 gttcgcatgc acctgaaggg gagacctcat gcaaatgcgt tacaaagccg ctctgctgac
    52261 gatcatcggt gcgctcgccc tgtatggttg cggtcaggac gaagccacct cctccgcgtc
    52321 cggtgacgcg tcgtccgacg acgtgatcac cgtcgtcatc ggtcacgttg ccccgctcac
    52381 cggcaacatc gctcatctgg gcaaggacaa tgagaacggt gcgcggctgg cggtggatga
    52441 tctcaacgcg cagcagctga aaatcggcgg caagacgctg gtgattcagt tgcagggcga
    52501 agacgaccag gccgatccga agaccgcgac tctcgtggcg caaaagctcg tcgattcgcg
    52561 cgttgccggc gtggtcggtc acctgaattc cggcacgacc attcccgcct cgcaaatcta
    52621 cgccgacgcg ggtattccgc aggtgtcgcc ttcggcgacc aacccgactt atacgctgca
    52681 gggcttcaaa acggcgttcc gcgtgatggc gaacgatatc cagcagggtt ccgtgctcgg
    52741 ccgctatgcg gtgaaaggcc tgggcgcgaa gcgcatggcg atcatcgatg accggaccgc
    52801 ctacggccag ggactggccg acgaggtgga gaagggggcg aaggcggccg gggcggaaat
    52861 cgtcgcgcgc gagttcaccc gcaatgacgc caccgacttc cgcgccatcc tcaccagcat
    52921 caagagccgc aatcccgatg tgatcttcta cggcggcatg gacgcgcagg ccggtccgat
    52981 ggccaagcag atgcgtcaat tgggcatgga tgcgaaattc atggcgggtg acggcggctg
    53041 ctcgcccgaa ttcatcaagc tcgccggcga cgccgcggaa ggcacgtact gctcgcagac
    53101 cggcctgccg ctggagaaga tgccgcgcgg gcccgcgttc gcgaagcgtt acatggagaa
    53161 gttcggcagc ccggtgcaga tctactcgcc ttattcgtat gacgcggtgc aggtgctggt
    53221 cgacgccatg gtgcgcgccg ggtccatcga tccggccaaa tacctgcccg agatcgcgcg
    53281 cacgagtttc gaaggtgtga gcggccgcgt cgaattcgac gagaaaggcg atttgcgcaa
    53341 cggcgccgta acagtgtatg tagtgcgtaa cggcaaacat gaagtgcgcg aaatcgtcgg
    53401 cggcagcccc atcgatcagg ccgctgcggc ggtgcgcgca aacatcgatg ccgcaaagct
    53461 cgccggggac agcgcggcgc gcgcggtcgg agaaaaagtg cggacggtga cgccccagcc
    53521 ctgaacgatg cggttgatgg atagacggca cctgcgggtg ccgtttgttt atccgcgctg
    53581 ctcctctcgc gtcacggcgc gcagaaccgc tgccagacca agaatatgat ctttcagttc
    53641 gatatcctgc tgcagcagat catcaacggc cttgtgctgg gcagtgtcta tgcgctggtg
    53701 gcgctcggct acaccatggt gtacggcatt ctcggcctga tcaatttcgc ccacggcgac
    53761 gtggtgatga tcggcgcgat gacggcactc accatcgcaa cggcgctgca gacctttgcg
    53821 ccggaaatgc cgacggtact ggtgctgctc gtcgcagtgg cgggcgccgc gcccgtatgc
    53881 atggcgctcg gctacaccat ggagcgcgtc gcctaccggc cgctgcgcaa cgcgccgaga
    53941 ctggccccgc tgatcaccgc gatcggtatt tccatggtgc tgcaggcggc ggcgatgatg
    54001 atctggggcc gcaactactt cacctttccc gagttggttt cgcatcaacc gatcatggtt
    54061 ctgggcgcga ccatcacgcc ggtgcagatc atgatcgtca cgctcgctgc ggcgctcatg
    54121 gccggtctga cgctgatggt caaccgcacg cgcatcggcc gcgccatgcg cgcgacggct
    54181 gagaatcaac cgattgcggc gctgatgggg gtgaatgtca acggcgtcat ttcgttcacg
    54241 ttcatcgtcg gctcggggct cggctgcgtc gccggcgtga tggtggcggc gaactacggc
    54301 atcgcgcact actacatggg gttcattctc ggcctgaagg ccttcaccgc cgcagtgctc
    54361 ggcggcatcg gcaacatcca gggcgccatg ctgggcggtc tgctgctggg tctcatcgaa
    54421 agtctgggcg ccggctacat cggcgatctg accggcggct tcctcggtag ccactatcag
    54481 gatgtgttcg cgttcttcgt gctcatcgtc gtgctgattt tcaagccttc ggggttgatg
    54541 ggcgaacgcg tggcggagcg cgcatgaccg accggtttgc ggcattgaag gcgcatccgg
    54601 ccgcgaagtg ggtcggtatg ctgctcatcg gcgcggcgct gctgttgctg cccttcgcgg
    54661 tcggtcatgt gggcgggccc gcgtgggtgc gcgtgctcga cttcgcgctg ctttacgtca
    54721 tgctcgcgct gggtctcaac atcgtcgtcg gtttcgccgg cctgctcgat ctgggctaca
    54781 tcgccttcta cgccgtcggc gcctacacct acgcgctgct cgcttcgccg catttcggca
    54841 tccatctgcc gctgtgggcg ctgctgccca tcgccgcggg agtggcagcc attttcggcg
    54901 tactgctcgg catgccggtg ctgcgtttgc gcggcgacta tctggccatc gtcacgctgg
    54961 gcttcggcga aatcatccgt atctttctca ataacctcaa cgcgccgatc aatatcacca
    55021 acggcccgca gggcatttcg cgcatcgatc cactcgccgt cggggatttc acgctcacca
    55081 agtcgcacac gctgttcggc atcaccttca gcggcgtgca ttggcactac tacttctttc
    55141 tcttgctcgc gctgctgatc attttcatct gcatgcgcct ggaggattca cgcatcggcc
    55201 gcgcctgggt ggcgatccgc gaagacgaag tggcggccaa ggcaatgggc atcaacacgc
    55261 gcaacatcaa gctgctcgcc ttcgccatgg gcgcgacttt cggtggagtg gcgggcggtc
    55321 tgtttgccgg cttccaggcc tttgtcagcc ccgagagctt catcctgatg gagtcgatcg
    55381 tcgtgctgtg cgccgtggtg ctcggcggca tggggaatat tccgggcgtc atcatcggtg
    55441 cattgatcct cgccacactg ccggagtttc tgcgctacac catcatgccg gcgcagcaag
    55501 cattgttcgg gcagcagatc gtcgatcccg aagtgctgcg caccatgctg ttcggattgg
    55561 cgctggttct ggtcatgctg ttccgtccgg cgggcctgtg gccctcccaa gtgcgaaagc
    55621 gggagctggc acgatgaccg tgctgctgga agcccgcgcc atcgacaagc gcttcggcgg
    55681 tttgcgcgcc ctcaccggcg tttcgctgcg catggagcgc ggacagatct acggcctcat
    55741 cgggccgaac ggtgcgggca agacgacctt cttcaactgc ctcaccgggc tttaccggcc
    55801 ggacggcggc gagttccgct tcgatggcga gccgctcgcc accgatgccc cgcacgaagt
    55861 cgcgcggcga ggcatcgcgc gcacgttcca gaacatccgt ttgtttgcgc acatgagcgc
    55921 gatcgaaaac gtcatggtcg gccgccacgt gcgtacccat gcaggcgtga tcggcgcagt
    55981 gctgcgcggg ccgcgcacgc gcgccgagga gcgcgcgatt cacgagcgtg cctacgcgct
    56041 gctcgactac gtcggtctgg ctgcacggca gcacgaacag gccaagaacc tgtcctacgg
    56101 cgaccagcgc cgactggaga ttgcgcgtgc gctggcgacc gagcccaagc tgctggcgct
    56161 cgacgaaccg gccgccggca tgaatgcgac cgaacgcgag gctttgcgcg cgctcctgtg
    56221 cagaatccgc gacgacggcg tcagcctgct gctcatcgaa cacgatgtcg cgctggtcat
    56281 ggggttgtgc gaccgcattg cggtgctcga ttacggcaag accattgccg aaggcctgcc
    56341 ggccgaggta cagcgtgatc cggccgtcat cgaagcctat ctgggcggga cactggcatg
    56401 aacgcgccgt tgttagcggt ggagggcttg cgcgttgcct acggcggcat tcaggcggtc
    56461 aagggcatcg atctggaagt gcgtcgcggc gaactggtgg cgctgatcgg tgccaacggt
    56521 gcgggcaaga ccacgacttt gaaagccttg atgcgcatgc tcccctgggc cggcggcacg
    56581 gtgcgttatg ccggacacga tctgcgtgcg acgccgctcc attgcatggc ggagaagggc
    56641 atcgcgctgg tgccggaggg gcgcggcgtg ttcgcgcgcc tgagtgtgga agagaatctg
    56701 gaaatgggcg cctacagccg ccgcgatacg catgcgatcg cgaccgacat ggcgcgaatg
    56761 tacgaattgt tcccccgcct gtacgagcgc cgcgatcaac tggccggtac gctgtccggc
    56821 ggcgagcagc agatgctggc catggggcgc gcgttgatgt gccggccgca attgttgctg
    56881 ctggacgagc cgagcatggg gcttgcgccg gtgatcgtgc agcgcatttt cgaaatcgtg
    56941 cacgagatca accaggcggg taccaccatt ctgttggtgg aacagaacgc caagctggcg
    57001 ttgcaactgg cgacgcgcgc ctatgtgatg gagagcggac tcatcacact gagcggtgat
    57061 gcgccggtgc tggcgcgcga ccccaaggtg cgcgccgcct atctgggcga agtcgccggt
    57121 tagacaaact cagcttgagc ggcggatcag ccgacgtgct tccgcttggc cagatcggcc
    57181 gcggtggtga aggagtcggc atagaactcc tccagcggca ggccgcgctc gtgatggaag
    57241 tcgtgccgcg ccgattccac catgaccggt gagccgcagg cgtagacctg atggccggaa
    57301 agatcggcgt agtcttccat cacggcgtga tgcacgaaac cggtgcggcc cgtccagtcg
    57361 tcttcgggca aggcctcgga gacgacgggc acgaagcgga agttcgggtg ttcggccgcc
    57421 cagcgctcgg cgagctcggt catgtacaga tccttcggcc gccgaccgcc ccagtagagc
    57481 accatgtcgc gggtcgtgcc tttctggaaa ctcgcctcga gaatcgcctt gaccggtgcg
    57541 aagccggtgc cgctggcaac gaacaccacg ggcttgtcgt cgtcttcacg caggaagaag
    57601 gagcccagcg ggccctcgaa gcgcatgatc tcgcgctcct tcatggtgtt gaacacgtga
    57661 tcggtgaacg cgccgcccgg aatgtggcgg atgtggacct ggatcgacgc gtcgtcatgc
    57721 ggcggattcg ccagcgagaa gcttttgcgc gtgccgtccc ggagcaggaa gtcgatgtac
    57781 tgacccggca ggaattgcag ccgttcgttc gccggcagcg acagatgcag caccacgacg
    57841 tcgtccgcga cgcggtcgat cttctgcacg cggcagggca gcttgcgcac gacaatgtcc
    57901 ttcaccgcgt tgatctcgcg gctttcgatc accagatcgc cgcgtggctt ggcgacgcag
    57961 aacagcgcga aacctgcggc gcgttcttcg tcggtgagcg ccgtttcctg ataggtgccg
    58021 taatcgattt cgcccgacag cagtttgccc ttgcacgagc cgcaagcccc gttgcggcaa
    58081 ccgtagggca ggtgaaagcc gtcgtccagc gcggcgtgca ggacggtttc gtcttcttcg
    58141 acggtgaaag tattgccgct cggttgaatg gtgactttgt agctcatgtc cggtgtagat
    58201 aggggccggt agggcaagcg ctgcccacct tgccgacatt ccttagaatg acgtgatgga
    58261 acgactgctg atcgtcggtt gcggcgatat cgcgcgccgc gccttgccgc aactgctgac
    58321 gcgctacgcc atccgtgcgt tggtgcggtc ggcggcggac gcgtcggcgc tgcgcgattg
    58381 cggtgtggag cccgtcatcg cggatctcga ctgccccgat tcgttgaagg cgctgagtga
    58441 aggggcggac gcgctcgtgc accttgcgcc gcccggcgat gaaggatcgc gcgacctgcg
    58501 gacccgcaat ctgacggccg cgttgtcggc gtcgggcatg ttaccacggc gcactgtgta
    58561 catcagtacg agcggtgtct acggcgattg cggcgggcgg cgggtggacg agacccgccc
    58621 ggtcaacccc tcgaccccgc gtgcccggcg gcgggtcgat gcagagcagg tcctgacgcg
    58681 ctggcaaccg gagacggtca tcctgcgtgt gccgggcatt tatgcggcgg accggctgcc
    58741 gctcgagcgc ctgcggcgcg gcacgccggt gttgcgcgac gaggacgacg tgtacaccaa
    58801 tcatatccat gccgacgacc tggccgccgc ggtatgccgc gcgctggaac atgtgcccgc
    58861 cggcgccgtc tataacatca gcgacgacag cgagctgaaa atgggcgaat ggttcgactt
    58921 cgtcgccgac cgctatggcc tgccgcggcc gccgcgcgtc tcgcgcgccg aggcgagcgg
    58981 cgtgattccg cccatgctgt tgtctttcat gaacgagtcg cgtcgcttgt cgaatgcccg
    59041 catgaaggcg gaactgggtc tgcgcctgcg ctaccccacg gtgcgcgaag gcgtgcccgg
    59101 cccatccaca gaggaatgaa cgatgtcatc accgtccacg ctgctggtct tctccaatct
    59161 gcccgatcgc gcgagcgccg agacgctcgc ggcggcgctg gtgcagcgac aggcggccgc
    59221 ctgtgtgaat atcatggcgc cctgccgctc ggtttaccgc tggcagggtg cggtggaaca
    59281 gagcgaggaa attccggtgt tcatcaagac cacggcggat cgctacgaac tggtcgaaca
    59341 catcgtgcgc gagttgcacc cctacgaact gccggagatc atcgccctgc ccgtcacggg
    59401 cggactgcct gcctatctgg cgtgggtggc ggccgaaacc cggcccgaca attgagaaag
    59461 ggaacaggaa cttgaacgga atctggcgct ggtgcctgct gctgctcggc ctgtgggccg
    59521 gtatggcgac ggcgcaggag gagcctgagc tgctcgaacc ggatcgcgcc ttcgaactga
    59581 gcgtgcgaca ggtcgaaccc gcggcgctcg aggtgcacta ccgcatcgct gacggctatt
    59641 acatgtatcg cgacaagttc gcgttcgagg ccatgccggc gagcgtgcag ctggggcagc
    59701 cggtgttccc gcccgggcag gtgcatgacg acgaattctt cggccgcacg gaaacctatc
    59761 gcggcgagct gtccattcgc attcccgtgt ccggttcggc ggaacgtgcg caactgcgtg
    59821 tcacttcaca gggctgcgcc gatatcggcg tgtgctatcc gccgcaccgg caggtgcgca
    59881 ccttccagct tgccgcagcg gacggcggcg ggaatgtcgg cggcacgagc gcggtcgagc
    59941 cggcagcggc cgccggatcg gtcaacgagt tgctgcgctc gggccggacg gtacgcaacg
    60001 aagcgccgat gttcagcgac gaggcgcgct ttacggacgc gctggaaagc ggccgcctgc
    60061 cgctgatcgt cagtctgttc ttcgtggccg gcatcctgct caccttcact ccttgcgtac
    60121 tgcccatgat tcccattctt tccgggctga tcgtgggcga aggccggcag gtgacgcgga
    60181 cgaacgccac ggtactgtcc atcgcctacg tgctcggcat ggtggtgacc tatacggcca
    60241 tcggggtggg tgcggcgctg tccggtgcct ttttcgccgc ggcgctgcag aacccgtggg
    60301 tgctcggcgc cttcgcgctg atcttcgttg cgctggcgct gtcgatgttc gacttctacg
    60361 acattcccat cccgttccag tcgcacgcca tcgcggcgag tggcaagctg ccgggcgggc
    60421 gcatcggtgc cgtggcggtg atggggatgc tgtctgccgc catcgtcagt ccttgtgtgg
    60481 ccgcgccgct tgcgggcgcc ctgctgtaca tcagccagac acgcgatgcg ctgctgggcg
    60541 gcagtgcgct gtttgccatg gcgctcggca tgggcgtgcc gctgatcgcg gtcggcgtga
    60601 ccggcggcgc gttgctgccg aagtccggcc actggatgaa ggcggtgaag aaatttttcg
    60661 gctttctgct gctcggtgtg gcggtgtgga tcgtctcgcc gctgctgccg gtggcggcgc
    60721 agatgacggc ctgggcgacg ctgttgatcg tcggcgcgat gtttctgcac gcgctcgatc
    60781 ccctgcccgc gcaggcggcc ggcgtggcgc gtttctggaa gggcatcggg gtgattgcgc
    60841 tgctcgtcgg cgcttcgctg ctcgtcggcg cgctctcggg tggccagagt ctgttgcgcc
    60901 ccctggatgc ggtgggtggc cgggtggcga aggcagcaga gccgaacgtc gtccgcttcg
    60961 agcgcgtgcg cgatgtcgcc gaactcgagg cgcgactggc tgccgccacg caacccgtcc
    61021 tgctcgactt ctatgccgac tggtgcgtgt cctgcaagga aatggaggcc ttcactttta
    61081 ccgatccgca agtgcgtgag cgcatgggac aaatgcattt gctgcaagtg gacgtgacgc
    61141 gaaactcggc ggctgatcag gcactgctca agcgcttccg gctgttcggg ccgccggcaa
    61201 tcattttctt caccgccgag gggcaggaaa cgggcgcgcg cgtgatcggc tatcagccgg
    61261 cgcggcgctt ccagcagacg ctggacgggg tgctcaacgg ctgatcagtg cgacgcgcgc
    61321 accgcgagca cgatcttgcc gatgtgttgc gaggattcca tcaactcgtg cgcagcgacg
    61381 acgtcgtgca acggaaaggt ctggtggatg cgcgtgcgca accgcccgga ttcgaacagc
    61441 ggccacacgt gctcgcgcaa accgttcgcg atcccggctt tctgttcgac gctgcgcgcc
    61501 cgcaacgtcg agcccatgac ggtcaaacgg cgcatgatga tgtacatcgc attgaagctg
    61561 gcgatctcgc cgccccggat ggcggcgatg agcgccagcc ggccttccat ggccagcgag
    61621 cgcaggttct tcatgaagta gtcgccgccc accatgtcga gaatgacgtt cacgccccgc
    61681 ttaccggtgg ccggccacag cgccgccgcc caatcctcgg tgcggtagtt gtagaccacg
    61741 tcggcaccat attcgcggca caccgccgcc ttctcggcat tgcgtacggt ggtatagacg
    61801 gtcgacgcgc cgcgcgcctt ggccagttgt atggcggcca ggccgatgcc gctggagccg
    61861 ccgtgcacca gaaaggattc gccggcctgc aacttcgccg actcgaagac gttggcccag
    61921 acggtaaagt aaacctcggg caaggacgcg gcttcgagca acgacaagcc cttgggcacc
    61981 ggcagacaat gcgcggccgg cacggcacag tactcggcgt aaccgccgcc gggcgccagc
    62041 gcacacaccc agtcgccgac gcgccattgg gtgacatcgg gcgcgcacgc caccacccgg
    62101 ccgcttactt ccaggccgag tatgggcgaa gcgccgggcg gcggcggata aacgccggca
    62161 cgctgggaga gatcggggcg gttcacgccg gcatagtgga cttcgatcag cacacggccg
    62221 ggccggactg cggggggcgg accctcgacc aggcgcatga cgcttgccgg gccgcaggat
    62281 ccatggtcga cgtacagcat cgtgctcact tcctcaggat cgcgcatggt agcgcatccc
    62341 ccgaagggaa ccgacgcggc tgtctataat gattttttag ccgtccatag gcgaagcgct
    62401 tggacgccat tccgcaattt gccaatcgca ccgtctttga cggccgtcat ctgctgtggg
    62461 cggtggtcgt gtcgctgatg ctgcacggcc tgttgatcgt cggcacgccg ccgctcgggt
    62521 tgttcgacta tcggccgccg atcgagcggc cgctcgaact cgacttgatt gccgcaccgc
    62581 cgccgccagc ggtcgtcgaa ccgtcgcatc ctgtgccgcc gcaacgtcgc gcccatgcgg
    62641 caccgcaacc gtccgaaccg gtcgcaacga cgcgtacgcc gcggcaggtc acggccgagc
    62701 ccatgcaggc ggcggtcaca caggaagacg aggcgggcag cgcaacggcg ccggcgctgg
    62761 gcgaaccggc ggtgccgatc gcaccgatcg tacaagcggc agcgtccgca ccggcggctt
    62821 atccgctgcg ctcggcgcgg ctggtgttcg acctttatta cggcgattcg cagacgctgg
    62881 tcggcgaagt agtgcaaacc ttcgaactgc aggacagccg ctatgtggcg acggcgagcg
    62941 cccaggcggt gggtttcgtg gggcttttcc tgcacggccg ttacgaacag cgttccgagg
    63001 gacggctcgg tcctcacggc atggtgcccg agcgttatac cgtcgaggag cggcgcgggc
    63061 ggggaaccga acacgcgacg ttcgactggt cggcaggcaa gctgcgcttc gagcgtaagg
    63121 ggcgggtgca cgaggcgccg ctcgaggccg gcgcgcagga tccgctatcg gcgatccatc
    63181 agctgcatta tttgcggccg ctggagccgg gactgctgat ggacgtggcg acggcgcgca
    63241 aagccgaacg gatgctgttc atgaacatgg gaca
//
LOCUS       DATGOU010000055        14785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_126900, whole genome shotgun sequence.
ACCESSION   DATGOU010000055 DATGOU010000000
VERSION     DATGOU010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14785
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126900"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..1553)
                     /locus_tag="VKZ48_03135"
     CDS             complement(126..1553)
                     /locus_tag="VKZ48_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HLU76882.1"
                     /translation="MTGAPPPVLSVISREAPLSLVEQIVRDVRSGIEDRRLRPGVRMP
                     SIRRYALTHGVSRFTVVEAYDRLVAQGYLESRRGSGFYVREQPQPLHPLAAEMEAAGS
                     RVDIAWLLRNTFTEHKGRDDLLPAGAGILPERWLDSELLNGALRTLSRRGDAAVVAYG
                     TPQGYAPLRQLLQVQLATLEIEAAPHQIVLTVGASQALALIARYFLKPGDPVLVDEPG
                     YYTLFGRLAAMGVRLVGVPRTADGVDTARLEELVLRHKPRLYITNSVLHNPTGTSLSA
                     AAAFRILRLAETHDFYICEDDVYADLYPGRAHRIAALDQLNRVLYVGSFSKTLGAGLR
                     VGFLACRAELARDLADQKMLVSLTSPELGERLVHQILAEGQYRKAVERLRSRISRAMG
                     PAMRNLESAGLRLFHEPGAGMFLWARVPTETDTTPIAAAARAQGIVLAPGSLFSPTQA
                     PSPWMRFNVAYAGNPSLARFFHSLE"
     gene            1654..2625
                     /locus_tag="VKZ48_03140"
     CDS             1654..2625
                     /locus_tag="VKZ48_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76883.1"
                     /translation="MFLRLAALLFFSLVLSACDQSARTLALPADFYPSALAIDPVHGR
                     YLVGSFHNGEVAVLDRTGRRLGTLRKPAPGERVLRLRVEGRTLWVALPRTIEAIDLDD
                     PQRPRAVYRLQASVHYADMAIAGDRIYLLDAREGQVYALDHAQRVAYRYAALPERAAR
                     PQPAACLLSDETAEPAGALLLTPQRDALLIALNGALYRLDLVSRRIEAVRMHLPMTHV
                     SQLLYRGLHAGRHEVVAIRGMAARTTHLRLDASFRRVLAQESFRVASAAPMAAAADGA
                     RLRLVLGSLRHHPLHCGDGRPNTAIRLVSYPSDKTAEAWRLAAARDE"
     gene            2679..3164
                     /locus_tag="VKZ48_03145"
     CDS             2679..3164
                     /locus_tag="VKZ48_03145"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HLU76884.1"
                     /translation="MQTEYQAILPTPFGALGVLTQDDAVREIVYLPPETAPLAPQNAL
                     AERACAQLQRYVADPDYIFDLPLCTAGTPFQRAVWREICAVPRGQVTRYADIARKLSS
                     AARAVGQACGANPFPPVVPCHRVVSANGLGGFANHTDGYLMATKRWLLRHEGWLANPL
                     L"
     gene            3140..4063
                     /gene="xerD"
                     /locus_tag="VKZ48_03150"
     CDS             3140..4063
                     /gene="xerD"
                     /locus_tag="VKZ48_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HLU76885.1"
                     /translation="MAGQSAALSEADAALLDAFCDQLWLEDGLAKNTLEAYRRDLRLF
                     GEWLARTGRGLLEASEGDLLGYLAARHAAAQRPQASTQARALSSFKRFYHYALRERRI
                     SVDPTLKLDRPKKPSRFPKSLSEAEVEALLDAPDVETPLGLRDRAMLETLYATGLRVS
                     ELVALKFAETNLDMGVVRVFGKGGKERLVPLGEVAVEWLTRYLKNARPLLAKGRLNDA
                     MFLTPRGEPMTRQAFWYLIKRHARAAIPGVALSPHTLRHAFATHLLNHGADLRVVQML
                     LGHADISTTQIYTHVARERLKRLHAIHHPRG"
     gene            complement(4072..4431)
                     /locus_tag="VKZ48_03155"
     CDS             complement(4072..4431)
                     /locus_tag="VKZ48_03155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HLU76886.1"
                     /translation="MDLIFMRDFRLEAMIGIYKRERVTTQPISIDLDIAVPDARSFTS
                     GDVADTVDYGVVAQRLREELATRRFGLVEEMAEFIADLLLTEFRSPWVRVTLSKRGIL
                     KDVREVGIVIERPRAKA"
     gene            4530..5159
                     /gene="plsY"
                     /locus_tag="VKZ48_03160"
     CDS             4530..5159
                     /gene="plsY"
                     /locus_tag="VKZ48_03160"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HLU76887.1"
                     /translation="MTGAAFILTAYLIGSLSFAILVSRVMGLPNPHSYGSGNPGATNV
                     LRSGNKTAAMLTLLGDAGKGWIAVFLAKHLAWQFDVSDAVIAAVALAVFIGHLYPVYF
                     GLRGGKGVATAGGILLALDWRVGVGTLATWLIIAFFFRYSSLAALVSAVFAPFFTALV
                     IGVDAYVAATVAIAALLVWRHRSNIRKLLDGTESRIGEKKKDAATAAAD"
     gene            complement(5156..6208)
                     /gene="tsaD"
                     /locus_tag="VKZ48_03165"
     CDS             complement(5156..6208)
                     /gene="tsaD"
                     /locus_tag="VKZ48_03165"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HLU76888.1"
                     /translation="MVQPFASSSPARLVLGIETSCDETGIALYDTHAGLLAHAVHTQV
                     AMHAEYGGVVPELASRDHIRRILPLIETVLAEAGKSLSELEAIAYTEGPGLAGALLVG
                     ASVANALAFGLGIPAIGIHHLEGHLLSPLLSKPAPTFPFAALLVSGGHTQLMEVEGVG
                     RYTLLGETLDDAAGEAFDKTAQLLGLGYPGGPALSRLADQGRPGRYKLPRPMLNSPDL
                     DFSFSGLKTAVLTLTRGMTLNDENRADVARAFQDAVVDVLVAKCRAALEQTGLNQLVV
                     AGGVGANRALREALGKAAERDGFRLYYPELELCTDNGAMIALAGALRLEAADSTEAAT
                     GYRFTVRPRWDLATLA"
     gene            complement(6255..7028)
                     /locus_tag="VKZ48_03170"
     CDS             complement(6255..7028)
                     /locus_tag="VKZ48_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLU76889.1"
                     /translation="MFKRLLHRPGYRIELSLLGLAVLVLAWSMGAQLLKDSIALANLL
                     SPGKTFDSLYQLLAQNQLTAHTITSMKRVLVGLGLALALGVPIGLAIGASRALENATS
                     SAFQFLRMISPLSWMPIAVMVFGIGDAPIYFLLTFAAVWPIILNTAAGVRQLDPQWLM
                     LARSLSATPSEVLFKIVIPGILGHILTGVRLAIGIIWIVLVPCEMLGVSSGLGYFILD
                     TRDRLAYSELMAVIVLIGALGFMLDGIAQKLHHRWTRAA"
     gene            complement(7054..8256)
                     /locus_tag="VKZ48_03175"
     CDS             complement(7054..8256)
                     /locus_tag="VKZ48_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLU76890.1"
                     /translation="MKKIPTSEATREARRQFLKLSAMFTAAGALPLLQTYNQAIAAEP
                     DAPLRIGYLPITDATALLVAHHNGLFQKQGLEVEKPRLFRSWAQIVEAFLAGQVNAVH
                     LLSPMTVWARYGSKSQAKVVAWNHMSGSGLTVLPEIDSVAELGGKTVAIPFWYSIHNV
                     VLQHLFRGEGLESISDGTPNAKQVKLVVMAPSDMVPALATRQIAGYIVAEPFNAQAEV
                     LKVGKILRFTGDVWRDHACCVVLMHENDLNRRPEWTQKVVNGIVQAQAWIRTHRKETA
                     QILSRDNAQRYTPHTYEALEHVLAPEEKMYAHYASSGAIRHPQWKDERISFQPYPYPS
                     YTETLVKMLQKTVVLGNSEFLKTLDPAFAARDLVDDRFVKKAIADLGGMKVFGQAEAY
                     TRTETITA"
     gene            complement(8264..9076)
                     /locus_tag="VKZ48_03180"
     CDS             complement(8264..9076)
                     /locus_tag="VKZ48_03180"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLU76891.1"
                     /translation="MSAPQPKVVLEAQGIELTYQRPNQPDNTVLRDFSMRLAAGEVVA
                     VLGPSGVGKSSLLRVLAGLQEPAQGTVRVHGETLNGPHPRLSFVFQDPCLLPWLNLEQ
                     NVAFGLDFKHQPQLSAEQKQHRVRSAIAEVDLSHARERYPAELSGGMAQRTALARGLA
                     RQPEILLLDEPFSALDEITRGEMQALLQQITARHRTAAVLVTHDIDEAMIVADRILLI
                     GGAPGRLIGQWRIDMPHPREDVLPDLTYMRVEIVQALRDARRSTRSTVPLAA"
     gene            complement(9080..9244)
                     /locus_tag="VKZ48_03185"
     CDS             complement(9080..9244)
                     /locus_tag="VKZ48_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HLU76892.1"
                     /translation="MAQWMCTPCGYIYDEAVGMPEDGIPPGTPFEAIPDTWRCPDCGV
                     TKADFFLVPD"
     gene            complement(9244..10359)
                     /locus_tag="VKZ48_03190"
     CDS             complement(9244..10359)
                     /locus_tag="VKZ48_03190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLU76893.1"
                     /translation="MTQPLAHPQIDAITELVKRTLAPLASAIDQEGVYPETFLRELGA
                     LNGYNSAIAPAYGGLGLDLSPQLAVTTLVGRECGSTAFLVWCQSTVAWYLQHAPNEAV
                     RNRYLTAVAKGELLAGTGMSNPVKHLAGIERINLTAERDGDDYIVNGALPWVSNVGEG
                     HLAVVAAAVGDEGYVMFAVRGGTPGLELHPCPAFSGMEGTRTLNLRFKDLHIGGDDVL
                     AHPHQFPAFIRRIKPGFVLGQIGMGTGVIEGSLKTIRESNASHAHVNVYLDHQGNELA
                     EELAQLQAAVTALVPQAQDGSAPIVDVLRARAHASELTLKAANSAVLHAGARGYLMRH
                     PAQRRLREAVFVAIVTPALKHLRKEIDEIERQQAEAA"
     gene            complement(10395..10937)
                     /locus_tag="VKZ48_03195"
     CDS             complement(10395..10937)
                     /locus_tag="VKZ48_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HLU76894.1"
                     /translation="MSRLTIQTIESAPAEAKPRLENVKKNSGFVPNLLGVLANAPTAL
                     ETYQTVSAINARNGLTGAEREVVQITAATRNGCGFCVAGHTKLARKALNMPEDIVNAL
                     RNTAALSDPKLNALAVFTLAVIDKKGQVSDEELKRFFDAGYTQANALEVVLGVSLATL
                     CNYANNLAQTPINPELQAYA"
     gene            11145..11357
                     /gene="rpsU"
                     /locus_tag="VKZ48_03200"
     CDS             11145..11357
                     /gene="rpsU"
                     /locus_tag="VKZ48_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HLU76895.1"
                     /translation="MPSVRVKENEPFEVALRRFKRTVEKTGLLTELRAREYYEKPTAE
                     RKRKHAAAVKRHYKRLRSQQLPPKLY"
     gene            11433..11879
                     /locus_tag="VKZ48_03205"
     CDS             11433..11879
                     /locus_tag="VKZ48_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HLU76896.1"
                     /translation="MSLKTRITDDMKNAMKAKDAQRLGALRLLLAAVKQREVDERTEL
                     TDTDVIAVIDKMLKQRRDSIAQYQAAGRQDLADQERFEVGVLSAYMPEQADDSAIAAE
                     IDAAIQATGAKGPADMGKVMGAVKAKLAGRADMARVSAQVKARLTA"
     gene            12027..13814
                     /gene="dnaG"
                     /locus_tag="VKZ48_03210"
     CDS             12027..13814
                     /gene="dnaG"
                     /locus_tag="VKZ48_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HLU76897.1"
                     /translation="MNLDSFKRDLLNRVDIVDVINSRVPLKKGGANWMACCPFHSEKT
                     PSFTVSPSKQFYHCFGCGAHGNAIGFLMEYAGLGYIDALRELAESVGMKLPEYERPKR
                     GPAREEGPDLREYMARAARYYRDQLKQAPQAIEYLKGRGLTGQIAARFGIGYAPDDWQ
                     NLEAVFGKYDSKDGRALIDCGLVIENDNGRRYDRFRGRVMFPIVDQRGAVIGFGGRVI
                     GQGEPKYLNSPETPLFEKGRELYGLAQARQAIRQANRVIVVEGYMDVVALAQHGVENA
                     VATLGTATSAIHVQKLLRQADEVVFCFDGDEAGRRAAWHALEVSLEALSDRKAVKFLF
                     LPAEHDPDSYVRELGQKAFQRAVVQADPLSAFLLAQLRARGDLRSLEGRSRLIAEAKP
                     LLKRIAAPALQLQLLKTLAEEVGMAPAEVGRLTEIRMPSVSAERRAPAAQPRGGADSR
                     LERELLRCIEMQPELARQVAIEWVDLRTAEGRAVAALIELVQHDGSTEWNSAALIDHF
                     RDTEHSAVLSAVQSENLSRAANADDVAVVFDHTVAKMQARVRRARALELGAKIKSGVA
                     TEEEKREFVHLSSRAAAPAGLVSSAGDPT"
     gene            13885..>14785
                     /locus_tag="VKZ48_03215"
     CDS             13885..>14785
                     /locus_tag="VKZ48_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HLU76898.1"
                     /translation="MAREKSKAKPIAKSKAAAKPVAKTARPVAKAKAAPSRAAAAKAT
                     VAKSKTVTKVAAKKTATKSVSRTAAASKAKAPAKSATKTATKAVAKTVKPAAKKTAAK
                     TAVPAKKAAAPAKKTAATAKAKSVAPKKAAAVKTPAVKTRAPEKPVKTKAVTKAQVKN
                     DKTKVETPAPVKPLKVNKAKAREAERALIARADQKPEDAEARRMRLKSLIKLGKERGY
                     LTYAEINDHLPEVVDAEQIESMISTFNDMGIQVYDEAPDSETLLMAEGTPKSASDDDA
                     EEEAEAALATVDPEFGRTTDPVRM"
BASE COUNT         2615 a         4642 c         4959 g         2569 t
ORIGIN      
        1 ttgatcgacg agcgtggtct tgccgtgatc gacgtgggcg atgatggcga tgttgcgtaa
       61 ggcgcgggac atgtaaaaag ccttgcgagg agcgcgggga aacccgcaat tctaacaggc
      121 ggcgcctact ccagcgaatg gaagaagcgc gccaaactcg ggtttccggc ataggccacg
      181 ttgaagcgca tccagggcga gggcgcctgc gtcgggctga acagactgcc gggcgcaagc
      241 acgatgcctt gtgctcgcgc ggctgccgcg atcggggtgg tgtcggtctc ggtcgggacg
      301 cgcgcccaca ggaacatccc cgcgcccggc tcgtggaaaa ggcgaaggcc cgcgctttcc
      361 agattgcgca tggccggccc catggcgcgc gaaatacggc tgcgcagccg ttcgacggcc
      421 ttgcggtact gcccctccgc cagaatctgg tgcaccagcc gctcgcccag ttcgggcgaa
      481 gtcaggctga ccagcatctt ctgatcggcc agatcgcgtg ccagctccgc ccggcaggcc
      541 aggaagccga cccgtagtcc ggcgcccagc gtcttggaga agctgccgac gtagaggacg
      601 cggttgagct gatccagcgc ggcgatgcga tgcgcgcggc ctggatacag atcggcgtac
      661 acgtcgtctt cacagatgta gaagtcgtgc gtttccgcca ggcgcaggat gcggaaggcc
      721 gccgccgcgc tgagcgaggt gccggtcggg ttgtgcagca ccgagttggt gatgtagagc
      781 cgcggcttgt ggcgcagcac cagttcttcc agccgggcgg tgtccacgcc gtcggcggtg
      841 cgtggcacgc cgaccagccg cacgcccatc gccgccagcc ggccgaacag cgtgtagtag
      901 cccggctcgt ccaccagcac cggatcgccc ggcttgagga agtagcgcgc gatcagggcg
      961 agcgcctgac ttgcgcccac ggtcagcaca atctgatgcg gcgcggcttc gatctccaac
     1021 gtggccagtt gcacttgcag cagttggcgc aggggtgcat agccttgcgg cgtgccgtag
     1081 gcgacgaccg ccgcatcgcc gcgccgggag agcgtacgca gtgcgccgtt cagcaactcg
     1141 ctgtccagcc agcgctcggg caggatgcct gcgccggcgg gcagcaggtc gtcgcgcccc
     1201 ttgtgctcgg tgaaggtgtt gcgcagcagc caggcgatgt cgacgcgcga cccggccgct
     1261 tccatctccg cggcgagcgg gtgcagcggt tgcggctgct cgcgcacata gaagcccgag
     1321 ccgcgccgcg attccagata gccctgtgcc accagacggt cgtaggcttc caccaccgtg
     1381 aagcgcgata cgccgtgcgt cagcgcataa cggcggatcg agggcatgcg gacgcccggc
     1441 cgcagccggc ggtcctcgat gccgctacgc acatcgcgca cgatctgttc gaccagcgac
     1501 agaggcgctt cgcgcgaaat gaccgacagc acgggaggcg gggcgcctgt catggcgatt
     1561 tctcctatac agttgagtaa actggcaccg taactgtaca tgtagtgtac aggtgcgcgt
     1621 cgctatcgta gcgtttcccg aacggaggcg cacatgtttc ttcgtcttgc cgccctgctg
     1681 tttttcagcc tcgtcctcag tgcttgcgat caatcggcgc ggaccctcgc cctgccggcc
     1741 gacttctatc catccgcgct ggccatcgac ccggtgcatg gccgctatct ggtcggcagc
     1801 ttccataacg gggaggtggc ggtcctcgac cgcaccggcc ggcggctcgg cacgctgcgc
     1861 aagccggcgc cgggcgagcg ggtcctgcgc ctgagggtgg agggccgcac gctctgggtg
     1921 gcgctgccgc gcaccatcga ggcgatcgat ctcgacgacc cgcagcggcc gcgtgcggtt
     1981 tatcggctgc aggcttcggt gcactacgcc gacatggcga tcgccggcga ccgcatctat
     2041 ctgctcgatg cccgggaagg tcaggtgtat gcgctcgatc acgcgcaacg agtggcgtat
     2101 cgctatgctg cgctgcccga gcgtgcggcc cggccgcagc cggcggcgtg cctgctgagc
     2161 gacgaaacgg ccgaacctgc gggcgcgttg ctgctcacgc cgcaacgaga cgccttgctc
     2221 attgctttga acggcgcgct ctaccggctg gacctcgtca gtcgccggat cgaggcggtg
     2281 cgcatgcacc tgccgatgac gcatgtcagc caactgctct atcgcggtct gcatgcgggc
     2341 cgtcatgaag tggtcgccat acggggcatg gcggcacgca cgacgcatct gcggctcgat
     2401 gcctccttcc gccgggtcct tgcgcaggaa tccttccggg tggcgtccgc agcgccgatg
     2461 gctgcggcgg ccgacggtgc acggttgcgc ttggtgctcg gctcgctccg tcaccatccg
     2521 ctgcattgcg gagatgggcg gccgaacacg gcgattcgtc tggtgagcta tccgtccgac
     2581 aagacggcgg aagcatggcg actcgccgcg gcacgcgacg agtagacagc gccgcgtacg
     2641 agttgacgca tcccgggcgc gcgcctattt ttgtgcctat gcagaccgaa tatcaggcca
     2701 tcctgccgac ccccttcggc gcgctcggcg tgctgacgca ggacgatgcg gtgcgcgaaa
     2761 tcgtctatct gccgccggag acggcgccgc ttgcgccgca aaacgcattg gccgaacgag
     2821 cgtgcgcgca actgcagcgc tacgtcgccg atcccgatta catattcgat ctgccgctgt
     2881 gtacggccgg cacgcctttc cagcgggcgg tgtggcgcga gatctgcgcc gtgccgcgcg
     2941 gccaggtgac gcgctatgcc gatatcgcgc gcaaactgtc gtcggccgcg cgggcggtgg
     3001 gacaggcttg cggcgcgaat ccctttccgc ccgtggtgcc ctgtcatcgc gtcgtatcgg
     3061 ccaacggtct gggcggcttc gccaatcaca ccgacggcta tctcatggcc accaagcgtt
     3121 ggctgttgcg gcacgaagga tggctggcca atccgctgct ctgagcgagg ccgacgccgc
     3181 gctgctcgac gccttctgcg atcagctttg gctggaggac ggtctggcga agaacacgct
     3241 cgaagcctac cggcgcgatc tgcgtttgtt cggcgaatgg ttggcgcgca ccggacgtgg
     3301 attgctggag gcgagcgaag gggatttgct cggttacctc gccgcccggc atgccgcagc
     3361 gcagcggccg caagccagca cgcaggcgcg cgcgttgtcg agcttcaagc gcttctacca
     3421 ctacgccttg cgcgagcggc gcatcagcgt cgatccgacg ctcaagctcg accgtccgaa
     3481 aaaaccctcg cgtttcccca aatccttgag cgaggcggag gtggaggcgc tgctggacgc
     3541 accggatgtg gaaacgccgc tgggcctgcg cgatcgcgcc atgctcgaga cgctgtacgc
     3601 caccggcttg cgcgtgtccg agctggtcgc tttgaagttc gcggaaacga atctggacat
     3661 gggcgtggtg cgcgtgttcg gcaaaggcgg caaggaacgg ctggtgccgc tgggcgaggt
     3721 ggcggtggaa tggctgacgc gctacctgaa gaacgcgcgc cccttgctgg ccaagggccg
     3781 gctgaacgac gcgatgtttc tcacgccgcg cggcgagccg atgacccgtc aggccttctg
     3841 gtatctcatc aagcggcacg ccagggcggc gatccccggc gtggcgctat cgccgcacac
     3901 gctgcggcat gccttcgcca cgcatctgct caatcacggc gccgacttgc gcgttgtgca
     3961 aatgctgctc ggtcacgcgg acatctccac gacgcagatc tacacgcacg tcgcgcgcga
     4021 gcggcttaag cgcctgcatg cgatccatca tccgcgcggc tgaacggcgt ctcacgcttt
     4081 ggcgcgcggc cgctcgatga cgatgccgac ttcgcgcacg tccttgagaa tgccgcgctt
     4141 gctcagcgtc acgcgcaccc agggagagcg gaattcggtg agcagaaggt cggcgatgaa
     4201 ctccgccatt tcctccacca ggccgaaacg gcgcgtcgcc aactcttcgc gcagacgttg
     4261 cgccaccacg ccgtagtcca ccgtatcggc gacgtcgccc gaagtgaagg aacgtgcgtc
     4321 cggcacggcg atgtccagat cgatactgat cggctgggtg gtgacgcgct cgcgtttgta
     4381 gatgccgatc atcgcctcca ggcgaaaatc gcgcatgaat atcagatcca tgaatctccg
     4441 gtcgatgcct tcgcgctaca cttgaggcgc cggtcggtca gcgaagctga caccatgccc
     4501 ggcgcacgtt tctacctcgt tcaaagatga tgactggcgc ggctttcatt ctaactgcgt
     4561 acctgatcgg ttcgctgtct ttcgcaattc tggtcagccg cgtgatgggg ctgcccaacc
     4621 cgcacagcta cggctccggc aatcccggtg cgaccaatgt gctgcgctcg ggcaacaaga
     4681 ccgcggccat gctgacgctg ctcggcgacg cgggcaaggg ctggatcgcc gtgtttctgg
     4741 ccaagcacct ggcgtggcag ttcgatgtga gcgatgcggt gatcgccgcc gtggcgctgg
     4801 ccgtcttcat cggccatctc tacccggtct atttcggcct gcgcggcggc aaaggcgtgg
     4861 caacggccgg cggcatcctg ctcgccctcg attggcgggt gggcgtgggt acgctggcga
     4921 cgtggctgat catcgccttc ttcttccgtt attcctcgct ggccgcgctg gtgtcggcgg
     4981 tgttcgcgcc tttcttcact gccctggtga tcggcgtgga tgcgtatgtg gccgcgacgg
     5041 tcgcgatcgc cgcgctgctg gtgtggcgcc atcgcagcaa catccgcaaa ctgctggacg
     5101 gcaccgaaag ccggatcggc gagaaaaaga aggacgcggc gacggccgcg gcagactagg
     5161 cgagcgtcgc gagatcccaa cgcggccgca cggtgaagcg gtagccggtc gcggcttcgg
     5221 tcgaatcggc cgcttccaga cgcagcgcgc cggccaaggc gatcatcgcg ccgttgtcgg
     5281 tgcacagttc cagttcgggg tagtacagcc ggaagccgtc gcgctccgcc gctttgccga
     5341 gcgcctcgcg cagtgcgcgg ttggcgccca cgccgccggc gacgaccagt tgattcaacc
     5401 cggtctgctc gagcgcggcg cgacacttcg ccaccagcac gtccaccacc gcatcctgaa
     5461 atgcccgcgc cacatcggcg cggttttcgt cgttcagcgt catgccgcgc gtcagggtca
     5521 gcacggcggt ctttaatccg ctgaagctga aatccagatc gggactgttg agcatcggcc
     5581 gtggaagttt gtagcggccc ggccgcccct gatccgccag acgcgacagg gccggaccgc
     5641 cggggtaacc gagccccagc agttgcgccg tcttgtcgaa agcttcgccc gccgcgtcgt
     5701 cgagcgtttc gcccagcagg gtatagcgcc cgacgccttc cacctccatc agttgcgtat
     5761 ggccgcccga gacgagcagc gcggcgaaag gaaatgtcgg cgcaggcttc gacagcagcg
     5821 gcgagagcaa atggccttcc agatgatgaa tgccgatcgc cgggatgccg agcccgaagg
     5881 cgagtgcatt cgcaacgctc gcacccacca gcagcgcgcc ggccaggccc ggcccctcgg
     5941 tgtaggcgat cgcttccagc tcggacagcg atttgccggc ttcggccagc accgtttcga
     6001 tcagcggcag gatgcggcgg atgtgatcgc gcgaagccag ttcgggcacc acgccgccgt
     6061 actcggcgtg catggcgact tgcgtatgca ccgcatgtgc gagcagaccg gcatgcgtgt
     6121 cgtagagcgc gatgccggtt tcgtcgcagg aggtctcgat gccgagaacg agccgtgcgg
     6181 gcgaggaaga agcgaaaggc tgcaccatgc cgcctagcgt acccgatccg cggcgcggcg
     6241 ggcggtttcg ccggtcaggc ggcgcgcgtc caacggtggt gcagtttctg tgcgatgccg
     6301 tcgagcatga agcccagggc gccgatgagc acgatcaccg ccatcagttc ggagtaggcg
     6361 aggcggtcgc gcgtatcgag gatgaaatag cccaggccgg agctgacgcc cagcatttcg
     6421 caaggcacca gcacgatcca gatgatgccg atggccagcc gcacgccggt gaggatgtgg
     6481 ccgagaatgc cgggaatgac gatcttgaac agcacttcac tgggcgtggc gctcaggctg
     6541 cgcgcgagca tgagccattg cggatcgagc tgccgcacgc ccgccgcggt gttgaggatg
     6601 atcggccaca ccgccgcgaa ggtcagcagg aagtagatcg gcgcatcgcc gatgccgaag
     6661 accatgaccg cgatgggcat ccacgacagc ggcgaaatca tgcgcaggaa ttggaacgcg
     6721 ctgctggtgg cgttctccag tgcgcgcgag gcgccgatcg ccaggccgat cggcacgccg
     6781 agcgcgagcg ccagtccgag gccgaccagc acgcgcttca tgctggtgat ggtatgggcc
     6841 gtcaactgat tctgcgcgag taactgatac aggctgtcga aggtcttgcc cggactgagc
     6901 agattcgcga gtgcgatgct gtccttcagc aattgcgccc ccatcgacca ggccagcacg
     6961 agcacggcga ggccgagcag gctcagctcg atacggtagc cgggacggtg gaggagtcgc
     7021 ttgaacatac gcttgtcagc cgatcggggt ggctcaggcg gtaatggttt ccgtgcgcgt
     7081 gtaggcttcg gcctgcccga agaccttcat gccgcccagg tcggcgatcg ctttcttgac
     7141 gaagcggtcg tccaccaggt cgcgcgcggc gaacgcagga tccagcgtct tgaggaactc
     7201 gctgttgccc aacaccaccg tcttctgcag catcttcacc agcgtctcgg tgtagctggg
     7261 atagggatag ggctggaacg agatgcgctc gtccttccac tggggatgcc ggatcgcgcc
     7321 cgaagacgcg tagtgcgcgt acatcttttc ttcgggcgcc agcacgtgct ccagcgcttc
     7381 ataggtgtgc ggcgtgtagc gctgtgcatt gtcgcgcgac aggatctgcg ccgtttcctt
     7441 gcggtgagtg cggatccagg cctgcgcctg cacgatgccg ttgaccacct tctgcgtcca
     7501 ctcgggacgg cggttgagat cgttctcgtg catcagcacc acgcagcaag cgtggtcgcg
     7561 ccagacgtcg ccggtgaagc gcaggatttt gccgaccttg agcacttcgg cctgggcatt
     7621 gaaaggctcg gcgacgatgt agccggcgat ctggcgggtt gccagtgcgg gcaccatatc
     7681 cgacggggcc attaccacca gcttcacctg cttggcgttg ggcgtgccgt cgctgatcga
     7741 ctccaggcct tcgccgcgga acagatgctg cagcacgacg ttgtggatcg agtaccagaa
     7801 cggaatggcg accgtcttgc cgcccagttc ggcgaccgag tcgatttccg gcagcaccgt
     7861 gaggccggag ccggacatat ggttccacgc caccaccttc gcttgcgact tgctgccgta
     7921 gcgcgcccac acggtcatgg gcgagagcag atgcacggca ttgacctgac cggcaaggaa
     7981 ggcctcgacg atctgtgccc aactgcggaa cagccgcggc ttttcgactt ccaggccctg
     8041 cttctggaac agcccgttgt ggtgcgcgac cagcagcgcc gtcgcgtcgg tgatcggcag
     8101 atagccgata cgcagcggcg catccggttc ggcggcgatc gcctggttgt aggtctgcag
     8161 caggggcagc gcaccggctg cggtgaacat ggcggacagc ttgagaaatt gacgccgggc
     8221 ttcgcgggtc gcttcggatg tcggaatttt tttcatggtg tgttcaggct gcaagtggga
     8281 cggttgatcg ggtactgcga cgcgcgtcgc gcagcgcctg cacgatctcc acgcgcatat
     8341 aggtgagatc cggcaggacg tcttcgcggg gatggggcat gtcgatgcgc cactggccga
     8401 tgagccggcc gggcgcgccg ccgatgagca ggatgcggtc ggcgacgatc atggcttcgt
     8461 cgatgtcgtg cgtgaccagc acagcggcgg tgcgatggcg tgcggtgatc tgctgcagca
     8521 gcgcctgcat ttcgccacgg gtaatttcat ccagcgcact gaagggctcg tcgagcagca
     8581 ggatttccgg ctgacgcgcg agaccgcgcg ccagtgccgt gcgttgcgcc atgccaccgg
     8641 acagttcggc gggatagcgt tcgcgcgcat gcgacagatc gacttcggcg atggccgagc
     8701 gcacgcgatg ctgtttctgt tcggcgctca gttgcggctg gtgtttgaaa tccaggccga
     8761 aggcgacgtt ctgttccagg ttgagccagg gcagcaggca cgggtcctgg aacacgaagc
     8821 tcagacgcgg gtgcgggccg ttcaaagttt cgccatgtac gcgcaccgtg ccctgcgccg
     8881 gctcctgcag gccggcgagt acgcgcagca gcgacgactt gcctacgccg ctgggcccca
     8941 gtaccgcaac gacttcgcca gcggccagtc gcatggagaa atcgcgcagc acggtattgt
     9001 ccggctgatt ggggcgctga taagtcagct cgatcccctg cgcttccagc acgacttttg
     9061 gttgtggggc gctcatcgat cagtccggta cgagaaagaa atcagccttg gtcacgccgc
     9121 agtcggggca gcgccaagtg tcgggaatcg cttcgaacgg cgtgccgggc gggatgccgt
     9181 cttcgggcat gccgaccgct tcgtcgtaga tgtagccgca gggcgtgcac atccattgcg
     9241 ccattacgcc gcctcggcct gctgccgttc gatttcgtcg atttccttgc gcaggtgctt
     9301 gagcgcaggc gtgacgatgg cgacgaacac cgcttcgcgc agtcgccgct gcgcgggatg
     9361 acgcatgaga taaccgcgcg caccggcgtg cagtacggca gagttggctg ccttgagcgt
     9421 gagttccgag gcgtgtgcgc gggcacgcag cacgtcgaca atcggcgcgg agccgtcctg
     9481 cgcttgcggg acgagtgctg tcaccgccgc ttgcaactgc gccagttcct cggccagttc
     9541 gttgccctga tgatcgagat agacgttcac atgcgcgtgg ctcgcgttgc tttcgcggat
     9601 ggttttgagg ctgccttcga tgacgccggt gcccatgccg atctggccga gcacgaagcc
     9661 gggtttgatg cgccggatga aagccggaaa ctgatgcgga tgcgccagca cgtcgtcgcc
     9721 gccgatgtgc agatccttga agcgcaggtt gagcgtgcgc gtgccttcca tgccggagaa
     9781 ggcggggcag ggatgcagtt ccaggccggg tgtgccaccg cgcacggcga acatcacata
     9841 accttcgtcg ccgacggcgg ccgcgaccac ggccaagtga ccttcgccca cgttcgacac
     9901 ccacggcagc gcgccgttga cgatgtagtc atcgccgtcg cgctcggccg tcaggttgat
     9961 gcgctcgatg ccggccagat gcttgaccgg attggacatg cccgtgccgg cgagcagttc
    10021 gcctttggcc acggctgtga gataacggtt gcgtaccgcc tcgttcggcg cgtgctgcag
    10081 ataccacgca acagtcgatt ggcaccacac cagaaaggcg gtcgagccgc actcgcggcc
    10141 gaccagtgta gtcactgcca gttgtggcga aagatcgagg ccgaggccgc cgtacgcggg
    10201 ggcgatggca ctgttgtaac cgttcagcgc gccgagttcg cgcaggaagg tttccggata
    10261 gacgccttcc tgatcgatcg cgctcgccag cggcgcgagc gtgcgcttca ccagttcggt
    10321 gatcgcgtcg atttgtgggt gggcgagcgg ctgggtcatc tggatactcc ttcggcggac
    10381 ggccggcgtg ctgctcaggc gtaagcctgc aattcgggat tgatcggcgt ttgcgcgagg
    10441 ttgttggcgt agttgcacag ggtggcgagg ctgacgccga gcacgacttc cagagcgttg
    10501 gcctgggtgt agccagcgtc gaagaagcgc ttgagctcct cgtccgagac ctggcccttc
    10561 ttgtctatga cggcaagcgt gaacacggcc agcgcattga gtttcgggtc gctcaacgcc
    10621 gctgtgttgc gcaatgcatt gacgatgtcc tccggcatgt tcaatgcctt gcgggccagc
    10681 ttggtatggc cggcgacgca gaagccgcaa ccattgcgcg tcgccgcagt gatttggacc
    10741 acttcgcgct ccgcgccggt caggccgttg cgcgcgttga tggcgctgac ggtctggtag
    10801 gtctccagcg cggtcggcgc gttggccagg acgccgagta aattgggaac gaaaccgctg
    10861 ttctttttga cgttttccag acggggcttg gcttcggcag gggcgctttc gatggtttga
    10921 atggtcaggc ggctcatatc caactcgatt tgatgtgggt tatatgcccg cagattctat
    10981 taatgtggaa acttaattag aaatatgaat atggaatttg aatatgcgta tccggcatat
    11041 gggcaggaag gggcgctcgc gaggcgcttc ttgtctggac gggtggccaa aggctataat
    11101 cgcggacttt ccgctcatca cctaaacaac ggagccagca tttaatgccg agcgttcgcg
    11161 tcaaagaaaa cgaaccgttt gaagtcgccc tgcgccgctt caagcgcact gtcgagaaaa
    11221 ccggcctgct cacggaactg cgtgcgcgcg agtactacga gaagcccacc gcagagcgca
    11281 agcgcaagca tgcggccgcg gtcaagcggc actacaagcg gttgcgcagc cagcaactgc
    11341 ctcctaagct gtactaagag ctttagtccg aattcctctt acgccccacc atccgaacat
    11401 ttgggtggtg gggtttgcta ttggtggcca gcatgtccct caagacgcgc atcaccgacg
    11461 atatgaagaa cgcaatgaag gccaaggacg cccagcgttt gggcgccttg cgtctgctgc
    11521 ttgccgccgt caagcagcgc gaagtggatg agcgtaccga gctgaccgac acggacgtga
    11581 tcgccgtgat cgacaaaatg ctcaagcagc ggcgggactc tattgcgcaa tatcaggctg
    11641 ccggccggca ggatctggcc gatcaggagc gtttcgaagt cggcgtgttg agcgcataca
    11701 tgcccgagca ggcggatgac tccgcgatcg cagccgaaat cgatgcggcg atccaggcta
    11761 cgggtgcgaa aggtccggcc gatatgggca aggtgatggg cgcagtgaaa gcgaagctcg
    11821 ccggacgcgc cgacatggcg agagtttcag cgcaggtaaa agcccggctg acggcctgag
    11881 ccacgcgaag gccgcgtcgc cggcgcgaca aactgcgcat ctcggagacg atgcgccaca
    11941 cggcattcgc cgagcgctga aaataggggg tcgcgccgct tggcgcgcga tccggtgcgt
    12001 gccttgggcg atggtgtaga gcggacgtga acctggactc cttcaagcgg gatctgctca
    12061 atcgcgtcga catcgtcgat gtcatcaaca gtcgcgtgcc gctcaagaag ggcggcgcca
    12121 actggatggc gtgctgcccg ttccacagcg aaaaaacccc ttccttcacg gtcagcccga
    12181 gcaagcagtt ctatcactgc ttcggttgcg gcgcgcacgg caacgccatc ggtttcctga
    12241 tggaatacgc cggcctcggc tatatcgatg cgctgcgcga actggccgaa agcgtgggca
    12301 tgaagctgcc cgagtacgag cggccgaagc gcggccccgc gcgggaagaa ggtccggatc
    12361 tgcgcgaata catggcgcgg gccgcgcgct attaccgcga tcagctcaag caggcgccgc
    12421 aggccatcga gtacctcaag gggcgcggcc tcaccggaca gatcgccgcg cgcttcggca
    12481 tcggctatgc gccggacgac tggcagaacc tcgaagcggt attcggcaaa tacgattcga
    12541 aggacggccg cgcgctgatc gactgcggtc tggtgatcga gaacgacaac ggacgccgct
    12601 acgatcgttt ccgcggccgc gtcatgtttc ccattgtcga tcagcgcggc gcggtgatcg
    12661 gtttcggcgg gcgcgtcatc ggtcagggtg aacccaagta cctcaactcg ccggagacgc
    12721 cgctgttcga aaaagggcgc gaactctacg gattggcgca ggcgcggcaa gcgatccgtc
    12781 aggccaatcg ggtcattgtg gtcgagggct atatggacgt cgtcgcgctc gcccagcacg
    12841 gcgtggagaa tgccgtcgcc acgctcggca ccgcgaccag cgcaatccat gtgcaaaagc
    12901 tgctccggca ggcggacgaa gtcgtgtttt gtttcgacgg cgacgaggcc ggccgccgcg
    12961 ctgcctggca cgccctggaa gtgagtctgg aggccttgtc cgatcgcaag gcggtgaagt
    13021 ttctgttcct gcccgccgag catgatcccg acagttatgt gcgcgaactc gggcagaagg
    13081 cttttcagcg cgcggttgtc caggccgatc ccttgtcggc tttcctgttg gcgcaattgc
    13141 gcgcgcgcgg cgatctgcgc agtctggaag gccgctcgcg tctcatcgcc gaagccaagc
    13201 cgctgttgaa gcgtatcgcg gccccggcgc tgcagttgca actgctcaag acgctggccg
    13261 aggaggtcgg catggcgccg gcggaagtgg ggcggttgac ggaaatccgc atgccgtcgg
    13321 tgtctgccga gcgccgtgcg cctgcggcgc aaccgcgcgg cggcgcggat tcccgtctgg
    13381 agcgtgaatt attgcgctgt atcgagatgc agccggaact tgcgcggcag gtggctatcg
    13441 aatgggttga tctgcgaacg gccgaagggc gcgccgtcgc ggcgttgatc gagttggtgc
    13501 agcatgacgg cagcacggaa tggaactcgg cggcgctgat cgatcacttc cgcgacacgg
    13561 aacacagcgc ggtgctgagc gccgtgcaga gcgaaaacct cagccgtgcg gccaatgcgg
    13621 acgacgttgc cgtcgtcttc gaccatacgg tcgccaaaat gcaggctcgc gtacggcgag
    13681 cgagggcgct ggaactcggg gccaaaatca aatccggcgt cgcaaccgaa gaagagaagc
    13741 gcgaattcgt ccacttgagc agccgcgctg cggcacctgc aggccttgtc tcctcggccg
    13801 gtgaccccac ctgatgtgct ataatcgcgg acttttttgc gcccctttcg tttattccaa
    13861 gcgcctgatt tcggggacct atcgatggca agagagaaga gcaaggcaaa accgatcgct
    13921 aagagcaagg ctgcggccaa gcccgtagca aaaaccgcac gtccggtggc gaaggcgaag
    13981 gccgcgccaa gccgtgccgc ggccgccaag gccactgtcg ccaagtcgaa aacggtcacc
    14041 aaagtcgccg ccaagaaaac cgcgaccaag tccgtctcgc gcaccgctgc cgccagcaag
    14101 gcgaaggcgc cggccaagtc cgcaaccaaa accgcgacca aagccgtagc gaaaacggtg
    14161 aagccggcgg cgaagaaaac cgctgccaaa accgctgtgc cggcgaaaaa ggccgccgca
    14221 ccggcgaaga aaaccgccgc gacggccaag gccaagagcg ttgcgccgaa gaaagccgct
    14281 gcggtcaaga cgccggccgt gaaaacgcgc gcgccggaaa agccggtcaa gaccaaggcc
    14341 gtgaccaagg cgcaagtgaa gaacgacaag acgaaagtcg agacgcccgc gccggtcaag
    14401 ccgctcaagg tcaacaaagc gaaggcgcgc gaagccgagc gtgcactcat cgcccgcgcc
    14461 gatcagaagc ccgaggacgc cgaagcgcgc cggatgcggc tgaagagcct catcaagctg
    14521 ggcaaggagc gcggctatct gacctacgcc gaaatcaacg accatctgcc cgaagtcgtg
    14581 gacgccgagc agatcgagtc gatgatctcg accttcaacg acatgggcat ccaggtctat
    14641 gacgaggcgc cggattccga gacgctgctc atggcggaag gtacgccaaa gtcggcaagc
    14701 gacgacgacg ccgaggaaga ggccgaagcc gcactggcga cggtcgatcc cgaattcggc
    14761 cgcaccaccg acccggtgcg catgt
//
LOCUS       DATGOU010000056        12208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_1912827, whole genome shotgun sequence.
ACCESSION   DATGOU010000056 DATGOU010000000
VERSION     DATGOU010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12208
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1912827"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VKZ48_03220"
     CDS             complement(<1..568)
                     /locus_tag="VKZ48_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76899.1"
                     /translation="MSSFDRFSGFNGRRFNGGRVDRRSGLLGLDRPGFRRGAEFACRH
                     AALHFGVRLIHGDRLRFHRGGFGRDGCYRQGRAVVRCGGLLRCIPMFRVTFSALAAVS
                     AAAAPAATPAAPFAFGAFLCMRRSLAAAVDLRRRLLLHGLLLGVGLTYFALFTCFALA
                     LASLLALLTRLLTRLAPLVAAFTRLSCFA"
     gene            608..973
                     /locus_tag="VKZ48_03225"
     CDS             608..973
                     /locus_tag="VKZ48_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76900.1"
                     /translation="MPAEPVEEEQQVKAEPVEEAKPIAAEAEETPHADVPVHEPEVSP
                     PVAAPAAPEVHAPAATTSLPSTEELLEASGLVLIETKPGAVRPEPEVQESAPAAPRRR
                     RERRPPPPNEPLQQLETHK"
     gene            1360..3534
                     /locus_tag="VKZ48_03230"
     CDS             1360..3534
                     /locus_tag="VKZ48_03230"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HLU76901.1"
                     /translation="MTALVSAAVEAQPANAGAQPPRIGLALSGGGARGAAHIGVLKVL
                     EELRVPVHCIAGTSMGSIVGGAYASGLTPKEMEKLIVGTDWGEVFTDRPPRAEISIRR
                     KADDFKNLYAPEFGVTDKGVTVPKGVIAGVSVESFLREVTQSAAQITDFSLLPIPFRA
                     VAADIATGEEVVMSEGSLAEALRASMSLPGVVPPVERNGRLLVDGGIANNLPIDVVRN
                     LCADIVIAVDIGTPPMKREDIGSALSVAGQLINLLGQYTVNQQKRSLKQGDVLISPEL
                     GDIASTSFERQAEAIAIGEAAARAMTEQLRRYSIPEAQYAELRRKQQPAAYQLGKVDE
                     IRFEGLERTNDAVLRELARSKPGETLTEDEIAADLRRIYGRGDFEGVNYRITEQDGRR
                     ALVFLPHEKSWGPDYLRFGIALASDFDSEHYFNVLLNYKKTWLNRLGGEWSTDLQLGR
                     DRLLQTELYQPLNEANDLFVSGYGQLARSVRGIFIGDDRVAEYDTRERRVGLDTGFNF
                     STWGSSRIGLMYRHVEADVDTGAPTLPEGELNVSGVRFAFVADQLDRAYLARSGYYLE
                     ATGLFPRAGLGADTSYDRMHLRASGAKSFGPHTFNLSVFHGTDFHSDMAAFDAFVLGG
                     PLRLSGFPIDRFAGREYTFGRLMYYNRAVKLPSPLGAGLYLGASIEAAEVKRQFTEPG
                     GTGDLWSASLFLAADTFLGPMYLGYGVGDGGESSLFFFIGVP"
     gene            complement(3652..4149)
                     /locus_tag="VKZ48_03235"
     CDS             complement(3652..4149)
                     /locus_tag="VKZ48_03235"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HLU76902.1"
                     /translation="MNVPSRYSILFVCTGNICRSVTAEAVFRKLTRDAGLDRLVRVDS
                     AGTHGYHIGDAPDPRTQAAARRRGYDMSRLRARKIEQGDFHNFDLLLAMDDEHYRIMS
                     RMADPAAAHKIRMMMAYAGRAQHSVVPDPYYGGPDGFELVLDLLEDSCEGLLAEVRQE
                     LEAIR"
     gene            complement(4210..4767)
                     /locus_tag="VKZ48_03240"
     CDS             complement(4210..4767)
                     /locus_tag="VKZ48_03240"
                     /inference="COORDINATES: protein motif:HMM:NF040826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCAM0308 family protein"
                     /protein_id="HLU76903.1"
                     /translation="MSASIAFAGGSGPATNARWKMNFGSNVTRGRGAAPQQAARSLQT
                     YVEPKRKAVPAPASCSDCNAVYYEGQWRWGAVPLSANETRCPACERLRRRLPAATLTL
                     QGASVAKHRDEFKRVALKREQALQQDNPLQRIMDMVPNPRGMTITTTEVQLARDIGEA
                     LREVYGGSLVAEYDKQGMLQVRWAR"
     gene            complement(4865..6925)
                     /gene="uvrB"
                     /locus_tag="VKZ48_03245"
     CDS             complement(4865..6925)
                     /gene="uvrB"
                     /locus_tag="VKZ48_03245"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HLU76904.1"
                     /translation="MLPLLRTFPNSPFQLNQAFAPAGDQPEAIEKLVEGLNDGLAFQT
                     LLGVTGSGKTFTMANVIARMGRPTLVLAHNKTLAAQLYAEFREFFPNNAVEYFVSYYD
                     YYQPEAYVPSRDLFIEKDSSINEHIEQMRLSATKSLLERDDAIIVATVSCIYGIGDPV
                     DYHGMILHLREKERVSQRDIIARLTAMQYQRNDFEFKRGVFRVRGDVIDVFPAEHSET
                     ALRMTLFDDELDSLMLFDPLTGKTLQRVGRFTVYPSSHYVTPRATVLKAIERIKDELR
                     DRTKYFYDNHKLVEAQRIEQRTRFDLEMLNEMGFCKGIENYSRHLSGRQAGEPPPTLI
                     DYLPPNAIMFIDESHVTIPQVGGMYKGDRSRKENLVEYGFRLPSALDNRPLRFEEFER
                     MMRQAIFVSATPGPYENEKSAQVVEQVVRPTGLVDPVLEVRPASTQVDDLLSEINARV
                     AAQERVLVTTLTKRMAEDLTDYLREHGVKVRYLHSEIETVERVELIRDLRLGEFDVLV
                     GINLLREGLDIPEVSLVAILDADKEGFLRSDRSLIQTIGRAARHIHGTAILYADTITD
                     SMRRAMDETERRRRKQIAHNEAQGITPKGVSKRIKDIIDGVYAAGDARAELKAAESQA
                     KYESMSEKQLSREIKRLEKQMLEYARNLEFEKAAQARDQLAELKKRMLGVDQLPSDVH
                     SAAG"
     gene            6960..8174
                     /locus_tag="VKZ48_03250"
     CDS             6960..8174
                     /locus_tag="VKZ48_03250"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HLU76905.1"
                     /translation="MSSAAPALFANVEMAPRDPILGITEAYNADQNPNKVNLGVGVYY
                     DDNGKLPLLECVKRAEQEMVEKAAPRGYLPIDGLPAYDVAVQGLVFGADSEPVKSKRV
                     VTVQSLGGTGGLKIGADFLRRVNPNAELWISDPSWENHRALFEAAGFKVHTYPYYDPA
                     THGVKFDAMIECLRGLPAGSVIVLHACCHNPTGVDLTADQWTKVIETINAQGLVPFLD
                     IAYQGFGDGLDADGAVIRRFAEAGGPVFVSNSFSKSVSLYGERVGALSIVATSAEEAA
                     RVLSQIKRVVRTNYSNPPTHGSKIVATILSSPELRKVWEGELAHMRDRIKSMRTQLVE
                     KLKARVPNVNFDFVIKQRGMFSYSGLTKEQVHKLRDEYSIYAVDSGRICVAALNSKNI
                     DYVADAIAKVIA"
     gene            complement(8249..9988)
                     /gene="cysI"
                     /locus_tag="VKZ48_03255"
     CDS             complement(8249..9988)
                     /gene="cysI"
                     /locus_tag="VKZ48_03255"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HLU76906.1"
                     /translation="MSSQSSAEVDRSADITQPLDKLSADERLKAGSNQLRGTILESIA
                     EPLSGSVREADGKLLKFHGMYQQDDRDLREERRRQKLEPAYQFMIRVRLAGGVCTPEQ
                     WLKLSDLALAYGTPALRLTTRQTFQFHGVLKHNVKKIIGGLHEVLLDTIAACGDDSRG
                     VMASINPHLSALHREVLELARATSNHVIPRMRAYHEIWLDGEKYLSSEPEEPIYGKTY
                     LPRKFKIGFAIPPLNDIDVYSQDLGFIAIADDEKLLGFNVAVGGGMGRTDRAPETYPR
                     LADVIGYIPKEQLLHTAQTIMTVQRDYGNRANRARARFKYTVDDKGIDFIRAEIERRL
                     GFALEAPRPVKFTTNGDRYGWQQGTDGRWHYTLFIENGRVIDRDEVQLMTGLREIAKV
                     HDGEFRVTPNQNVIIANVAEANRKRIDALLERYRLDRNNRSSVIRLHAMSCVGLPTCG
                     LAMAESERYLPSLISKIEPLLERHGLADVPITLRMTGCPNGCARPYIAEIAFTGRAPG
                     KYNMYLGGGYHGERINRLYLENVGEERILPELDKLFAHYAKDRKKGEHFGDFVVRAGH
                     VRPVLHGSDSYDV"
     gene            complement(9998..11833)
                     /locus_tag="VKZ48_03260"
     CDS             complement(9998..11833)
                     /locus_tag="VKZ48_03260"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HLU76907.1"
                     /translation="MDMIPPPVPGGKVDGAERELLRALGRSLTAEQALWASGYFAGIA
                     EARSSFAVAVDPAAAAQSAVASGSEAAPVKIKILYATETGNAAGLARRIADRAQALGL
                     AADAEDLARYKTRELKNEQVLLFVASTHGEGEPPEPALPFYEFLAGRKAPKLEHVQYA
                     VLALGDSTYQFYCEAGKVLDRRLEELGAKRLHERIDCDVDYETEANPWIDAVLTKLQA
                     TAKAGGASAVAPAFVAAAPAAAVSVYGKSNPFPAEITESLRLTGRGSSKDTRHVEISL
                     EGSGLHYAPGDALGIVPQNDPVLVAQLIEVGGWSGSETVTVRSGETTLELALTREFEI
                     SALTPRFIEQWAEWAGAGELKKLAGDERAQFMARTHIVDLMQSRPARGLAAQDFVQAL
                     RGLQPRLYSIASSPELTPDEVHLCVAPVRYRLNGRDCNGVASTALADRLSVGDSIPVY
                     VQPNEHFRLPADPATPIVMIGAGTGVAPYRAFMQHREALGIDGRSWLFFGERNFRTDF
                     LYQLEWQTWLRDGLLTRADVAFSRDQDEKIYVQHRLLQQAAELYRWINDGAHIYVCGD
                     AAHMAGDVHAALLSVIAEQGGLSPERAKETLLEMQSAGRYQKDVY"
BASE COUNT         2161 a         3939 c         3858 g         2250 t
ORIGIN      
        1 ccgcgaagca cgagagccgc gtgaaagccg cgacaagcgg agcgagtcgc gtcagcaagc
       61 gagtgagcag cgccagcagg gacgcgagcg ccagcgcgaa acacgtgaac agcgcgaagt
      121 acgtcaggcc cacgccgagc agcaggccgt gcagcagcag gcgtctgcgc aaatcaacgg
      181 cagcggccaa actgcggcgc atgcagagga aggcgccgaa cgcgaacgga gccgccggcg
      241 tcgcggccgg cgcggccgcg gcggagaccg cagcgagcgc gctgaacgtc acacggaaca
      301 tcgggatgca tcgcagcaga ccgccgcagc ggacgacagc acggccctgc ctgtagcagc
      361 cgtcgcgacc gaatccgccc cggtggaagc gcaagcgatc cccgtggatc aggcgcactc
      421 cgaagtgcag cgcggcgtga cggcaggcga actcggcgcc gcgccggaag cccggccggt
      481 cgagcccgag caacccgctg cgccggtcga cgcgcccccc gttgaaacgg cgcccgttga
      541 agccgctgaa gcgatcgaaa ctgctcaacc tgaagcggcc gcagccgaaa tgcccgagcc
      601 acctgctatg ccggccgagc ccgttgaaga agagcagcaa gtgaaggccg agccggtcga
      661 agaggccaag ccgatcgccg cggaagctga ggagactccc cacgccgacg taccggtgca
      721 cgaacccgag gtatcgccac cggtggccgc accggccgcg cctgaggtgc acgccccggc
      781 cgcgacgacc tcgctgccga gcaccgaaga actgctcgag gcaagcggtt tggtgctgat
      841 cgaaaccaag cccggtgccg tacggcccga gccggaagtg caggagagcg caccggcagc
      901 gccgcgtcgc cggcgcgaac ggcgcccgcc cccgccaaac gagccgctgc aacaattgga
      961 aacgcataag taagcgtccc caacaaaaaa gccgctgttc gcagcggctt ttttttacca
     1021 gggcgttatg ccgccatgcg ggcgcaaggc ggtaagacct cggtccaatt tccgcgacat
     1081 acacggctcg accataaggc gtagacgatt ccgatcggtg tgtacgcgag ccagttcgtc
     1141 cgcttcggcc acaattccgc ttgcgcttcg cttcgtcgta cttgcgggac gccgacccgc
     1201 ggtaagtatg tctttggttc cattgtgtgc accggatgcg catgccagca tcgacacacc
     1261 cgaattggag ggggtgcggc aggcgtgcga gcgctatacg acgagccgac gccaaacgcg
     1321 cgcggatttc gttttctcgg atgcgcgctg ttcgcgtcgg tgaccgctct tgtctcggcg
     1381 gccgtcgagg cgcagcctgc gaatgccggc gctcaacccc cgcgtatcgg cctcgcgttg
     1441 tcgggcggcg gtgcgcgcgg cgctgctcat atcggcgtgc tgaaggtgct cgaagaactg
     1501 cgtgttccgg tgcattgcat cgccggcacc agcatgggct cgatcgtcgg cggcgcatat
     1561 gcctccggcc tgacgccgaa agagatggaa aagcttatcg tgggcaccga ctggggcgag
     1621 gtgttcacgg accggccgcc acgcgcggaa atatcgatcc gccgcaaggc cgacgatttc
     1681 aaaaatctct atgcgcccga gttcggcgtg acggataagg gcgttacggt tcccaagggg
     1741 gtcatcgccg gcgtctccgt cgaaagcttc ctgcgggagg tcactcagtc tgctgcgcag
     1801 atcaccgact tctcgctgct gccgattccg ttccgcgccg tcgctgccga catcgcgacg
     1861 ggggaggaag tggtgatgtc ggaaggcagt cttgcggagg ccttgcgcgc cagcatgtcg
     1921 ctgcctggcg tcgtgccgcc cgtcgaacgc aacgggcgat tgttggtgga cggcggcatc
     1981 gcgaacaatc tgccgatcga cgtcgtgcgc aacctatgcg cagacattgt catcgcggtc
     2041 gatatcggta caccgccgat gaagcgtgaa gacatcggtt cggcgctcag cgtggcgggg
     2101 caactcatca acctacttgg gcagtacact gtcaatcaac aaaagcgttc cctcaaacag
     2161 ggcgacgtgc tgatttcgcc cgaactgggg gatatcgcct ccaccagctt cgaacgccag
     2221 gccgaagcga tcgccattgg cgaagcagcg gcacgggcaa tgacggagca actgcgtcgc
     2281 tacagcattc cggaagcgca atatgcggaa ctgcggcgca agcaacagcc cgccgcctac
     2341 cagctgggca aggttgatga aatccgcttc gaaggtttgg aacgcaccaa cgacgccgta
     2401 ttgcgcgagc tggcacgttc gaagcccgga gagacgttga ccgaagacga gatagccgcc
     2461 gatctgcggc gcatctacgg tcgcggcgat ttcgagggcg tcaattaccg catcaccgaa
     2521 caggatggtc ggcgagcgct ggtcttcctg ccgcatgaaa aatcatgggg accggattat
     2581 ttgcggttcg gcatcgctct cgcctcggat ttcgacagcg aacactattt caatgtcctt
     2641 ctcaactaca agaaaacctg gctcaaccgc ctgggcggcg aatggagcac cgatctgcaa
     2701 ttgggtcgcg atcgcctcct gcagaccgag ctctatcagc cgctgaacga ggcgaacgat
     2761 ctgttcgttt ccggatacgg gcaactggca cgctccgtcc gcggcatttt cataggcgac
     2821 gaccgggtcg ccgagtacga cacgcgcgaa cggcgcgtcg ggctcgacac gggtttcaat
     2881 ttcagtacat ggggctcatc tcgtatcggc ctgatgtacc gccacgtcga ggccgacgtc
     2941 gataccggcg caccgacctt gcccgaaggt gaactgaatg tgtcgggcgt gcgcttcgcg
     3001 ttcgtcgccg atcaattgga ccgggcttat ctcgcccgct cgggctacta cctcgaagcg
     3061 accggactat ttccacgcgc cggcctgggg gcggatactt cgtacgatcg gatgcatctg
     3121 cgcgccagcg gcgcgaaaag cttcgggccg cacaccttca acctctcggt gtttcacggc
     3181 accgatttcc attcggatat ggcggctttc gatgccttcg tgctgggtgg cccgttgcgt
     3241 ttgtccggct tcccgatcga tcgcttcgcc gggcgcgaat acaccttcgg acggttgatg
     3301 tactacaacc gggccgtcaa gctgccgagc ccgctcggcg ccggcctgta tctgggcgcc
     3361 tccatcgaag ccgccgaggt caaacgccag ttcaccgagc ctggcggcac gggcgacttg
     3421 tggtccgcgt cgctgttcct cgcggccgat acctttttgg ggccgatgta tttgggctac
     3481 ggtgtcggcg acggcggcga aagcagtctg tttttcttca tcggcgtgcc gtgagaagcg
     3541 acccgcgggt gcctcgaccc agccatcgcg gcaaccgcct tcggcagtcg cgcgcggccg
     3601 gtgcaatccg cttcgttcgt acgtctcgga cgaccgctgg cgccggtcac ttcagcggat
     3661 tgcctccaat tcttgacgca cttccgccag caatccttcg cagctgtctt cgagcaggtc
     3721 cagaaccagt tcgaaaccgt cgggcccgcc gtagtacggg tccggcacga cgctgtgctg
     3781 cgcccgcccg gcgtaggcca tcatcatcct gatcttgtgc gccgcagcgg ggtcggccat
     3841 gcgcgacatg atccgatagt gttcgtcgtc catggccagc agcagatcga agttgtggaa
     3901 atcgccctgc tcgatcttgc gcgccctgag gcgcgacatg tcgtagccgc gccgccgggc
     3961 ggcagcttgc gtccggggat cgggggcatc gccaatgtgg tagccgtgcg tgccggcgga
     4021 gtccacgcgc accaaccggt cgaggccggc gtcgcgcgtc agcttgcgga acacggcctc
     4081 tgccgtcacg gagcggcaaa tattgccggt gcagacaaaa agaatggaat atcgggacgg
     4141 tacgttcata cgcccgagtt taccgaggta caacgccgac tgggccggtc gcggcccctc
     4201 ggtggccgct tagcgggccc aacgaacctg cagcatgccc tgcttgtcgt attcggcgac
     4261 caggctgccc ccataaacct cgcgcagggc ctcgccgatg tcgcgcgcca actgcacttc
     4321 cgtcgtggtg atcgtcatgc cgcgcgggtt cggcaccatg tccatgatgc gttgcagcgg
     4381 attgtcctgc tgcaacgctt gctcgcgctt cagcgcgaca cgcttgaact cgtcgcgatg
     4441 cttggccacc gaggcgcctt gcagggtgag cgtcgcggcc ggcagacggc ggcgcagccg
     4501 ctcgcatgcg gggcagcggg tttcgttggc gctcaacggt acggcgcccc agcgccactg
     4561 tccttcgtaa tagaccgcat tgcagtcgga gcaactcgcg ggcgcgggaa ccgccttgcg
     4621 cttgggttcc acataggttt gcagcgagcg agccgcctgc tgcggcgccg ctccacggcc
     4681 acgtgtcacg ttcgatccga aattcatctt ccacctcgca ttcgttgccg gaccgctccc
     4741 gccggcgaag gcgatcgatg cggacatcga tccgtgcgcc aaagatagcg acgcgcgaga
     4801 gcggcccttt gacggatgtc aagcatggat caggccggaa tgtgaaaaac cctgcgtacc
     4861 gttcttagcc ggcggcagag tggacatcgg agggaagttg gtcgacgccg agcatgcgct
     4921 tcttcaactc ggccagttga tcccgcgcct gggcggcctt ttcgaattcc aggttccgcg
     4981 cgtactccag catctgcttc tccaaccgct tgatttcccg gctgagctgc ttttcgctca
     5041 tggattcgta cttggcttgg ctctccgccg ccttcagttc ggcgcgcgcg tcgcctgcgg
     5101 cgtagacgcc gtcgatgatg tctttgatgc gtttggacac gcccttgggc gtgatgccct
     5161 gcgcctcgtt gtgggcgatc tgcttgcgcc ggcgccgttc tgtctcgtcc atggcgcggc
     5221 gcatggagtc ggtaatggtg tcggcataaa gaatggccgt accgtggatg tgacgcgcgg
     5281 cccggccgat ggtctggatc agcgagcggt cggaccgcag gaaaccctcc ttgtcggcgt
     5341 ccaggatcgc caccagcgac acctcgggaa tgtccagccc ctcccgcagc aggttgatgc
     5401 cgaccagcac gtcgaactcg cccaggcgca ggtcgcggat cagttcgaca cgctccaccg
     5461 tttcgatttc cgaatggaga taacgcacct tgacgccgtg ctcgcgcagg tagtcggtca
     5521 ggtcttccgc catgcgcttg gtcagcgtgg taaccagcac ccgctcctgc gccgcgaccc
     5581 gtgcgttgat ctccgacagc aggtcgtcca cctgcgtgga cgccggccgc acctccagga
     5641 ccgggtcgac caggccggtg ggccgcacca cctgctccac cacctgcgcc gatttctcgt
     5701 tctcataagg gcccggcgtg gcggacacga aaatggcctg ccgcatcatg cgttcgaatt
     5761 cctcgaagcg cagcggccgg ttgtccagcg ccgaaggcag gcggaagccg tactccacca
     5821 ggttttcctt gcgcgagcgg tcgcccttgt acatcccgcc gacctgcgga atggtgacgt
     5881 ggctttcgtc gatgaacatg atcgcgttgg gcggcaggta gtcgatcagg gtcggcggcg
     5941 gctcgcccgc ctgacggccc gacaaatgac gcgagtagtt ctcgatgccc ttgcagaagc
     6001 ccatttcgtt gagcatttcc agatcgaacc gggttcgctg ttcgatgcgc tgggcttcca
     6061 ccagcttgtg gttgtcgtaa aaatacttcg tccggtcgcg caactcgtcc ttgatccgct
     6121 cgatggcctt cagcaccgtg gcgcgcggcg tcacgtaatg gctggacgga tagaccgtga
     6181 agcgcccgac ccgctgcagc gtcttgccgg tgagcgggtc gaacagcatc aggctgtcga
     6241 gttcgtcgtc gaacagcgtc atgcgcaggg ccgtctccga gtgttcggcg ggaaagacgt
     6301 cgatcacatc gccgcgtacg cggaatacgc cgcgcttgaa ctcgaagtcg ttgcgctggt
     6361 actgcatcgc ggtcaggcgg gcgatgatgt cgcgctgcga aacgcgctcc ttctcgcgca
     6421 gatgcaggat catgccgtgg tagtccacgg ggtcgccgat accgtaaatg cacgagaccg
     6481 ttgcgacgat gatcgcgtcg tcgcgctcga gcagcgattt ggtcgccgac aggcgcatct
     6541 gctcgatgtg ttcgttgatg ctcgaatcct tctcgatgaa caggtcgcgg gacgggacgt
     6601 aggcttccgg ctggtagtag tcgtagtacg agacgaagta ctcgaccgcg ttgttgggga
     6661 agaactcgcg gaattcggcg tagagctgcg ccgccagcgt cttgttgtgg gcgagcacga
     6721 gcgtcggccg ccccatccgg gcgattacgt tcgccatggt gaaggtcttg ccggagccgg
     6781 tgacgcccag tagcgtctgg aacgccaatc cgtcgttcag tccttcgacg agtttttcga
     6841 tggcctccgg ctggtcgccg gccggtgcga aagcctgatt gagctgaaac gggctgttgg
     6901 ggaaagtgcg gagcaagggt aaaatcgtgg gctcgtccaa cgaaacccag tctatcacga
     6961 tgagctccgc tgcccctgcc ctgtttgcca acgtcgagat ggcgcctcgc gatcccattc
     7021 tcggtatcac cgaagcctat aacgccgacc agaacccgaa caaagtgaac ctcggcgtcg
     7081 gcgtctatta cgacgacaac ggcaagctgc cgctgctgga gtgcgtcaag cgcgccgagc
     7141 aggaaatggt ggaaaaggcg gcgccgcgcg gttatctgcc gatcgacggc cttccggcct
     7201 acgacgtcgc cgtgcagggc ctggtgttcg gcgccgacag cgagccggtc aagagcaagc
     7261 gcgtcgtgac cgtgcagtcc ctagggggaa ccggcggcct gaaaatcggc gccgacttcc
     7321 tgcggcgcgt caatccgaat gcggaactgt ggatcagcga cccgagttgg gaaaaccacc
     7381 gcgcgctgtt cgaggcggcc ggcttcaagg tccacaccta cccctactac gatccggcca
     7441 cgcacggcgt gaagttcgac gccatgatcg aatgcctgcg cggcctcccg gcgggttcgg
     7501 tcatcgtgct gcatgcctgc tgccataacc ccaccggcgt cgacctcacc gccgaccagt
     7561 ggaccaaggt catcgagacg atcaacgccc aaggcctggt gcccttcctg gacatcgctt
     7621 accagggctt cggcgacggg ctggacgccg acggcgcggt catccgtcgc ttcgccgaag
     7681 ccggcggtcc ggtgttcgtg tcgaactcct tctccaaatc cgtttcgctc tacggcgagc
     7741 gcgtgggcgc gctgagcatc gtcgccacca gtgccgagga agctgcgcgc gtgctgtcgc
     7801 aaatcaagcg cgtggtccgc accaactatt ccaatccgcc cacgcacggc agcaagatcg
     7861 tggccaccat cctgagcagc cccgagttgc gcaaggtgtg ggaaggcgaa ctggcgcata
     7921 tgcgcgatcg aatcaagtcc atgcgcaccc agttggtgga aaagctgaag gcccgcgtgc
     7981 cgaacgtcaa tttcgatttc gtcatcaagc agcgcggcat gttctcctac tccggcctca
     8041 ccaaggagca agtgcacaag ctgcgcgacg agtactcgat ctacgccgtg gattcgggcc
     8101 ggatctgcgt cgccgcgctc aattccaaga acatcgacta cgtcgccgac gcgatcgcca
     8161 aagtgatcgc ctgaacgcag tcgcccgcag ttaaagaaaa accgcgcagg ccgaaaggcc
     8221 gcgcggtttt ttatggcgcc tacgccgctc agacgtcgta cgaatcgctg ccgtgcagca
     8281 ccggccggac gtggcccgca cggaccacga aatcgccgaa gtgttcgccc ttcttgcggt
     8341 ccttcgcata gtgcgcaaac agcttgtcga gttcgggcag gatgcgctct tcgccgacgt
     8401 tttccagata cagccggttg atccgctcgc cgtgatagcc gccgcccagg tacatgttgt
     8461 acttgcccgg cgcgcgcccg gtgaaggcga tttcggcgat atacggccgc gcgcacccgt
     8521 tggggcaacc ggtcattcgg agggtgatcg gcacatcggc caggccatga cgctcgagca
     8581 gcggctcgat tttcgaaatc agcgagggta agtaccgctc gctttccgcc atcgccaggc
     8641 cgcaggtggg caggcccaca cacgacatgg cgtggagacg aatgacgctc gaacggttgt
     8701 tgcggtccag ccggtagcgc tccagcaggg cgtcgatacg tttcctgttc gcctccgcca
     8761 cgttcgcgat gatcacgttc tgattcggcg tcacccggaa ctcgccgtcg tgtactttgg
     8821 cgatttcgcg caatcccgtc atcagttgca cctcgtcccg atcgatgacg cggccgttct
     8881 cgatgaacaa ggtgtagtgc cagcgtccat ccgtgccctg ttgccagccg taacggtcgc
     8941 cgttcgtcgt gaacttcacg ggtcgcggcg cctccagcgc gaagccgagc cgtcgctcga
     9001 tttccgcgcg tatgaaatcg atgcccttgt cgtccacggt gtacttgaag cgcgcgcgcg
     9061 cgcggttggc gcgattcccg taatcgcgct gtacggtcat gatcgtctgc gccgtatgca
     9121 gcaactgctc tttggggata tagccgatga cgtcggccag gcgcggatag gtttcgggcg
     9181 cgcgatcggt gcgccccatt ccgccgccga ccgcgacatt gaagccgagc agcttctcgt
     9241 cgtcggcaat cgcgatgaag cccagatcct ggctgtaaac gtcgatatcg ttcagcggtg
     9301 gaatggcgaa gccgatcttg aatttgcgcg gtagataagt cttgccgtag atcggctctt
     9361 ccggctcgga tgacaggtac ttttcgccgt ccagccagat ttcgtgatag gcgcgcatgc
     9421 gcggaatcac gtggttcgaa gtcgcgcgcg ccagttccag cacctcgcga tgcagagcgg
     9481 acaggtgcgg gttgatggac gccatcacgc cacggctgtc gtcgccgcag gcggcaatgg
     9541 tgtccagcag cacttcgtgc aagccgccga tgatcttctt gacgttgtgc ttcagcacgc
     9601 cgtggaactg gaatgtctgc cgcgtcgtga ggcgtaaagc cggggtgccg taggctagcg
     9661 cgagatccga cagcttcagc cattgttccg gcgtgcacac gcccccggcc agccgtacgc
     9721 ggatcatgaa ctggtacgcc ggctccagtt tctgacgccg ccgttcctcg cgcagatcgc
     9781 gatcgtcctg ctgatacatg ccgtggaact tgagcagctt gccgtccgcc tcccgcaccg
     9841 agccgctgag cggctcggca atgctttcga ggatggtgcc gcgtaattgg ttgctgccgg
     9901 ctttcaggcg ctcgtcggcg ctcagtttat ccagcggctg ggtgatatcg gcgctacggt
     9961 cgacttccgc actgctttga ctactcaact cttcacctca ataaacgtct ttttgataac
    10021 ggccggccga ctgcatttcg agcagcgtct ctttagcgcg ctccgggctc agcccgccct
    10081 gctccgcaat gacgctgagc agcgcagcgt ggacgtcgcc ggccatgtgc gccgcgtcac
    10141 cgcacacgta gatgtgcgcg ccgtcattga tccaccggta cagttcggcg gcctgttgca
    10201 gcaagcggtg ctgcacatag atcttttcat cctgatcgcg cgaaaacgcg acatcggcgc
    10261 gggtgagcag accgtcgcgc agccaggtct gccattcgag ctgatagaga aaatccgtgc
    10321 ggaaattgcg ctcgccgaag aacagccagc tacggccgtc gatgccgagc gcttcgcggt
    10381 gctgcatgaa ggcgcgatac ggcgcgacgc cggtaccagc gccgatcatc acgatgggcg
    10441 tcgccggatc ggccggcagg cggaaatgct cgttcggttg gacatagacc ggaatgctgt
    10501 cgcccaccga cagccggtcc gccagagcgg tggaggcgac gccgttgcag tcgcgcccgt
    10561 tcaggcggta acgcaccggc gcgacgcaga ggtgtacttc gtccggcgtc agttccggac
    10621 tcgacgcgat ggaataaagc cgcggctgca aaccgcgcag cgcctggacg aaatcctgcg
    10681 ccgcgagacc gcgtgccgga cgcgattgca tgagatcgac tatatgcgtg cgcgccatga
    10741 actgcgcacg ctcgtcgccg gcaagttttt tgagttcgcc tgcgccggcc cattccgccc
    10801 actgctcgat gaaacggggc gtaagcgcgc tgatctcgaa ctcccgcgtc agcgccagct
    10861 ccagcgtcgt ctcgccgctg cgcactgtca ccgtctccga cccggaccac ccgcccacct
    10921 cgatcaactg ggccaccagc accgggtcgt tttgcggcac gatgcccaat gcatcgcccg
    10981 gcgcgtagtg cagtccggaa ccttccaggc tgatctcgac atggcgcgtg tccttggacg
    11041 agccgcggcc ggtaaggcgc aggctttccg tgatctctgc gggaaacggg ttgcttttgc
    11101 cgtagacgga aacggctgcg gccggcgcgg cggcgacgaa agcgggcgcg accgctgacg
    11161 caccgcccgc tttggcggtc gcctgcagct tggtcaggac cgcgtcgatc cacgggttcg
    11221 cctcggtctc atagtcgacg tcgcaatcga tgcgttcgtg cagtctcttc gcgcccaact
    11281 cctccaggcg gcggtcgagg accttgcccg cctcacagta gaactggtag gtcgagtcgc
    11341 ccagcgccag caccgcgtac tgcacatgct ccagcttcgg cgccttgcgg ccggccagaa
    11401 attcgtagaa cggcagcgcc ggctccggcg gctcgccttc gccgtgcgtg ctggcgacga
    11461 acagcaacac ctgctcgttc ttcagttcgc gcgtcttgta gcgcgccagg tcttccgcat
    11521 ccgctgccag accgagcgcc tgagcgcggt cagcgattcg gcgcgccagt ccggccgcat
    11581 tgccggtctc ggtcgcgtag agaatcttga tctttactgg cgccgcttcg gaacccgagg
    11641 ccacggcact ctgcgcagca gcagccgggt cgaccgctac ggcgaagctg ctgcgcgcct
    11701 ctgcaatccc ggcaaaataa ccgctggccc acaacgcctg ttcggcggtc agcgaccgcc
    11761 ccagcgcacg cagcaactcc cgttcggcgc cgtccacttt cccgcccggc accggcggag
    11821 gaatcatgtc catatttgtt ctttccttga cctgggtcgc ggtgtatcga ccgtccgatg
    11881 aacgcacgcg taacgacccg agaagggcga aactttagat tccgtgctcc gcaaaacaaa
    11941 caattcaatt gttctttcct tatgctccac gcgggcgata caggggtggc gtggcgcgaa
    12001 ttcggaagat gcggtgccta cgaatgaggc agtaaaagcg cacgcgaaat tcgccttcga
    12061 acggcatcgg gttcggggta tgaacgcagg tcttcggtac gcagatttcg ggggtaaaag
    12121 tttgaccggc ccgccgggga tcgctagaat cgcggtctct caattccccg atagctcagt
    12181 cggtagagcg acggactgtt aatccgca
//
LOCUS       DATGOU010000057         8889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_4127691, whole genome shotgun sequence.
ACCESSION   DATGOU010000057 DATGOU010000000
VERSION     DATGOU010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8889
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4127691"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2334)
                     /locus_tag="VKZ48_03265"
     CDS             complement(<1..2334)
                     /locus_tag="VKZ48_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLU76908.1"
                     /translation="MAQSPLLHKVLRLNGIAYVGAVHGIDYDAARDLLRSRRRFIQGT
                     AAGLALVTAPAWLSVAAQTPPRIAIVGAGLAGLTCAYRLRKAGLTPTVYDGAKRLGGR
                     CVTNRLAFPGQHVEEGGEFIDAQHAEIRALANELGVPLRNEGDDFDLARGAPIYYING
                     AVYTAAAVYQDLLAIRGRVLSDLAAAPFPTRWNQNTARGRALDEMSVAQWIQQYVPGG
                     SASRLGRLLTVAYVNEFGAETNQQSALNLIYTLGMSGEGPLGPLGDKDQRYRVSGGAD
                     RFITELAKRQGNANIHLHHRLTRLARNDDGTYVLRFTTPSGIKNITADHVVLALNFAA
                     LRFHVDFNAANFDSRKRMAIGKQAMGEHALLHVQFKERYWWNVLGKTGLGFGEPYQSV
                     WSPSRGQTGTPGLLTNSVGGPRARLLRQGSTSEHVKQLLAQLEHTLPGLSKMATGVHL
                     LSYWPAHTWTRGSRSYYAQGDRLLFGGYEAVRQGNVHFCGEHTSLTAQGTLNGAVETA
                     LRCAAEIVKDIEAGGGPIVVPPPDPDPEPEPEPEPQPEPGLPGWVPSSGRMKRLVTAN
                     TFASVDPCPSDSCTYNSTYNGIGNDMVLRSFSGSIYNPYYSTHGAIVIHGGGHGNYGG
                     NETYVFNLDDLRFTRLDNPTDHTSLLAKSVPGNLWNLTYCEYIDGQPASSHSFDNLTI
                     IPPGKGGGNKGSLLRPISQACGKESRMTGYSHIFDLANPSKRWSRFSTNAYNGNKAPP
                     TGAAAYDSKRNVVWQFVHGNAIVGKLDIASKKWTNYSM"
     gene            complement(2604..4349)
                     /locus_tag="VKZ48_03270"
     CDS             complement(2604..4349)
                     /locus_tag="VKZ48_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HLU76909.1"
                     /translation="MIALVEAHRAGLLRREHWMQNIVAGVIVAVVALPLAMAFAIASG
                     AKPEQGLYTAIIAGLIVSIAGGSRVQIAGPTGAFIVILFAITAEHGIDGLQIATFMAG
                     VILLVLGLARLGGVIKFIPAPVIVGFTAGIGVVIWVTQWKDFFGLPTPAGEHFHEKLW
                     SLLQSLPQLHPTTTILASIALALVMFSTRIPYMQRVPGPLVALVVVTLLQALFQFDGV
                     TTIGTAFGGIPLGLPSLQMPEVSFARIIELTGPAFAIAMLGAIESLLSAVVADGMAGT
                     RHDSNQELVGQGLANIAAPLFGGFAATGAIARTATNIRNGGNSPLAGIVHALTLVLIV
                     LFLAPLAVHVPLAALAAILFIVAWNMSDVPHVLKMIRRAPLADVVILLITFLLTVFAD
                     LVVAVNVGVILAMLHFLRRMASSVEVQQVTGQELREETGQIDLTDMPPGVLAYAVQGP
                     FFFGAVENFERALAGTRTDPRVLIIQLRRVPFVDATGLQTLEEVIRDMHRRGVRVLIA
                     GANPRVSFKLNKAGVIELVGEAHCHQRFADAVAQARRLSEWMAAGHSGPVASLLDISQ
                     RYFAAPPGDQAERNG"
     gene            4627..5382
                     /locus_tag="VKZ48_03275"
     CDS             4627..5382
                     /locus_tag="VKZ48_03275"
                     /inference="COORDINATES: protein motif:HMM:NF018420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HLU76910.1"
                     /translation="MTLQSTFASLCGAALIAAGGLYAGPAQAVPVALELALLVDVSGS
                     IDGDEYDLQKQGYINAFRNANVQNAIAGQSGGIAVTYIEWSGSGQQAQLVGWTHLTDA
                     ASADAFADALGLTSRAFSGQTNPDAAINYAVPLFDDNDFEGLRLVIDVSGDGTGNANN
                     TAAARDAALAAGINAINGLAIGGTGLEDWYNDYLRGGAGSFVIGVADFEDFEGAVMTK
                     LQREITGSVPEPGTLMLLGLATAALAFSRRRMG"
     gene            complement(5559..7127)
                     /locus_tag="VKZ48_03280"
     CDS             complement(5559..7127)
                     /locus_tag="VKZ48_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HLU76911.1"
                     /translation="MSQAAEQVVAPWWRGAVVYQIYPRSFADSDGNGIGDLAGIRQRL
                     DHIQRLGVDAIWLSPAYRSPMVDAGYDVSDHCDIDPLFGTLADMDRLIAETHARGMRI
                     LLDYVPNHTSDQHPWFIESRSSRDNPKRDWYIWRDRPNNWVAAFGAGSAWTLDERTGQ
                     YYLHLFLPEQPDLNWRHPEVIGAMYGVLNFWLDRGVDGFRIDAVHCIGKDPTFNDDPH
                     CAVGQPLMLHNDQPYSHEVCRGLRKFADAHPSHCVLVGEIFVRSTEAVLRYYGEQDGL
                     HMAFNFSHLDTPWDAVGARDRIREVEGLLGPKGAWPTWVLSNHDFPRHRTSFGGSMRR
                     ARAAAVMLLTLRGTIFLYQGEELGLEDAQVGPDEQVDPGGRDGPRAPIPWESIPPHGW
                     SGEKPWLPFPPHPSTHAAETQWQDEHSILHLYRRLLAARKASTALRYGDWEELSSPLE
                     VLAYRRRCGEDERVVCVNFSGRPQQFPLAGRWLIEVVSEGEGEGASYDGNLDAEQALV
                     LKRAGRESGAESRG"
     gene            7259..7849
                     /locus_tag="VKZ48_03285"
     CDS             7259..7849
                     /locus_tag="VKZ48_03285"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HLU76912.1"
                     /translation="MFEALPRDAWRAYRDQDFPTRSWTAEADVIRALAEGPRVHLISR
                     KRYGDFELHFDWCVAEGGNSGVLYRVSEDYEEPWHSGLEMQLLDDPRHPDGMNPTTAC
                     GALYDLLPSSLMEPVPPSMFVTACIVVRGTAVEHWLANQLVLSYDLSDPTFRRHVAQS
                     KFKDYPAFGTVPEGHIVLQHHGTGAAFRNLRIRELD"
     gene            complement(7853..8212)
                     /locus_tag="VKZ48_03290"
     CDS             complement(7853..8212)
                     /locus_tag="VKZ48_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HLU76913.1"
                     /translation="MLRILFWRALALVALAFGLVGAVLPVLPTVPFLIVAAWAASRGW
                     PQLEAWLLNHERFGPALRGWRERGTVPRRAKWLASALMSMSVIAVLYSPVPVWVKIGV
                     PVFLCGLALWLWLRPEP"
     gene            complement(8222..>8889)
                     /locus_tag="VKZ48_03295"
     CDS             complement(8222..>8889)
                     /locus_tag="VKZ48_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HLU76914.1"
                     /translation="QAKLLRTLQNGEIQRLGADAPRRVDVRIIAATNRQLRDGVRDGH
                     FRADLYHRLSVYPVHIPPLRERGSDVLVLAGHFLELNRARLGLRSLRLTPEAEQALCA
                     YQWPGNVRELEHVISRAALKAVSRGAERTEIVSLEPELLDLDTQQEAMLPLAQLVSAA
                     RLPATDDAQGPRSLREAVETAQRQCIRAALARCNDNWASAARVLGVDASNLHKLARRL
                     GMK"
BASE COUNT         1593 a         2939 c         2862 g         1495 t
ORIGIN      
        1 catcgaatag ttggtccact tcttcgaggc gatgtcgagc ttgcccacga tggcattgcc
       61 gtgcacgaac tgccagacca cgttgcgctt cgagtcgtag gcggcggccc ccgtcggcgg
      121 cgccttgttg ccgttgtagg cattggtgga gaagcgcgac cagcgcttgg acggattggc
      181 cagatcgaaa atatgcgaat agccggtcat gcgcgactcc ttgccgcagg cctgactgat
      241 gggccgcagc aggctcccct tgttgccgcc gccctttccc ggcggaatga tggtgagatt
      301 gtcgaagctg tgcgaactgg caggctgccc gtcgatgtac tcgcaatagg tgaggttcca
      361 taaatttccg ggcaccgact tggcgagcag cgaggtgtga tcggtgggat tgtcgaggcg
      421 ggtgaaacgc agatcgtcca gattgaagac ataggtttcg ttgccgccgt aattgccgtg
      481 cccgccaccg tggatgacga tcgcaccgtg ggtgctgtag tacgggttgt agatgctgcc
      541 gctgaaggag cgcagcacca tatcgttgcc gatgccgttg tacgtgctgt tgtaggtgca
      601 actgtcgctg ggacagggat cgacggaggc gaaggtattg gccgtcacca gccgcttcat
      661 gcggccgctc gatggcaccc aaccgggcag accgggttcg ggctgcggct cgggctcggg
      721 ttccggttca ggatccggat cgggcggcgg caccacgatg ggcccgcctc ccgcctcgat
      781 gtccttcacg atctcagcgg cgcagcgcag cgcggtctcc accgcgccgt tcaacgtccc
      841 ctgcgccgtc agcgaggtgt gctcgccgca gaaatggacg ttgccttggc ggaccgcttc
      901 gtaaccgccg aagagcagcc ggtcgccctg cgcgtaataa ctgcgcgaac cgcgcgtcca
      961 ggtatgggcg ggccagtaac tcaacagatg cacccccgtc gccatcttcg acaggccggg
     1021 cagcgtgtgt tcgagttgcg ccagcagttg cttcacatgc tcggatgtag aaccctgccg
     1081 caacagccgg gcacgcggtc cgccgaccga attggtcagc aggcccggcg tgccggtctg
     1141 tccgcgggac ggcgaccaca ccgactgata gggttcgccg aaacccaggc ccgtcttgcc
     1201 gagcacgttc caccagtaac gttccttgaa ttgcacatgc agcaaggcgt gttcgcccat
     1261 cgcctgcttg ccgatagcca tgcgtttgcg cgagtcgaaa ttcgccgcat tgaaatccac
     1321 gtgaaaacgc aaggcggcaa agttcagcgc gagcaccaca tgatcggccg tgatgttctt
     1381 tatcccgctc ggggtcgtga aacgcagcac gtaggtaccg tcgtcgttgc gcgcgagacg
     1441 cgtgagtctg tgatgcagat ggatgttagc gttgccttgc cgcttcgcca gttcggtgat
     1501 gaaacggtcg gcgccgcccg aaacgcgata tcgctgatcc ttgtccccca gcggacccag
     1561 gggcccctcg cccgacatac cgagggtata gatcaggttg agcgcgctct gctgattcgt
     1621 ttcggcgccg aactcgttca catacgcgac ggtcagcagt cgccccagac gcgacgcgct
     1681 gccgcccggg acgtattgct gaatccattg cgcgacgctc atctcgtcga gcgcacgtcc
     1741 gcgcgccgtg ttctggttcc agcgcgtcgg aaatggcgcc gcagccagat cgctcagtac
     1801 gcggccgcgt atggccagca gatcctgata gaccgctgcc gcggtataca ccgcaccgtt
     1861 gatgtaataa atcggcgcgc cgcgcgccag atcgaagtcg tcgccctcgt tgcgcaacgg
     1921 cacgccgagt tcgttcgcga gcgcacgaat ctccgcatgc tgcgcatcga tgaactcgcc
     1981 gccctcttcc acatgctgac cgggaaaggc caagcggttg gtgacacaac gtccacccag
     2041 acgcttggcg ccgtcgtaaa ccgtcggagt gagcccggct ttacgcagtc gataggcgca
     2101 agtgaggccg gcgagtccgg ccccgacgat ggcgatgcgc ggcggcgtct gcgccgcgac
     2161 gctgagccac gccggcgccg tgaccagcgc aaggcccgca gccgtgcctt ggataaagcg
     2221 ccggcgcgat cgcagcagat cgcgcgctgc atcatagtcg atcccgtgta ccgcgccgac
     2281 gtacgcgatg ccgttcaacc ggaggacttt gtgcaacagc gggctttgtg ccatgagccg
     2341 actccctgcg cggactggaa atgcaagcgc acgtcgccct tcagggcggc gcgtgggaag
     2401 tcctgaaagg cggcatgagc atcgtggtgt gtacagcgct tttgcgctga cgcagcacgc
     2461 gctatgccgc cgcgccgcca tgcggtctga acgaattgtt ttacaaggga tgcgaagcga
     2521 ggacgacgcg cgcctcggat gtgatggggc ttaccctgta agccggtgcg acatggcccg
     2581 atggccctgt cgcaaagacg cgttcaaccg ttgcgttcgg cctgatcgcc cgggggcgca
     2641 gcgaagtaac gctggctgat atcgagcaga ctggcgacgg gaccgctgtg tcccgccgcc
     2701 atccattcgg acaaccgtcg cgcctgcgct accgcgtcgg caaagcgctg gtggcaatgc
     2761 gcctcgccga ccagttcgat gacacccgcc ttgttcagct tgaacgacac ccgggggttc
     2821 gcgccggcga tcagcacgcg cacgccgcgc cggtgcatat cgcgtatcac ttcctccagc
     2881 gtctggaggc cggtggcgtc gacgaagggg acgcggcgca actggatgat cagcacgcgc
     2941 ggatcggtgc gcgtgcccgc gagggcacgc tcgaaattct ccaccgcgcc gaaaaagaac
     3001 ggcccctgca ccgcataggc gagcacaccg ggcggcatgt cggtcaggtc gatctggccc
     3061 gtctcctcgc gcagttcctg tccggtcacc tgctgcactt caacgctcga cgccatacgc
     3121 cgcaggaaat gcagcatggc gaggatgacg ccgacgttca ccgccaccac caagtcggcg
     3181 aacacggtca gcaggaaagt gatgagcagg atgaccacgt cggccagcgg cgcgcgccgg
     3241 atcatcttca gcacatgggg cacgtcgctc atgttccatg ccacgatgaa caggatggcc
     3301 gccagcgcgg caagcggcac atgcactgca agcggcgcga ggaacagcac gatcagcacc
     3361 agggtcagtg cgtgcacgat gccggcaagc gggctgttgc cgccgttgcg gatgttggtc
     3421 gccgtgcgcg caatcgcgcc cgtcgcggcg aagccgccga acagcggcgc ggcgatgttc
     3481 gccaggccct gccccaccag ttcctgattg gagtcgtgcc gcgtgccggc catgccgtcg
     3541 gcgaccaccg ccgacagcag cgattcgatc gcgccgagca tggcgatggc gaaagccggg
     3601 ccggtgagct cgatgatccg cgcgaagctc acctccggca tctgcaggct cggcagcccg
     3661 agcggaatcc cgccgaaggc cgtgccgatg gtcgtgacgc cgtcgaactg gaacagcgcc
     3721 tgcagcagcg tgacgaccac cagcgcaacc agcggtcccg gcacgcgttg catgtacgga
     3781 atgcgtgtgg agaacatgac cagcgccagc gcgatcgacg ccaggatcgt ggtggtggga
     3841 tgcaactgcg gcagggactg cagcagcgac cagagctttt cgtggaaatg ctccccggcg
     3901 ggcgtcggca gaccgaagaa atccttccac tgcgtcaccc agatcaccac gccgatgccg
     3961 gcggtgaagc cgacgatgac cggcgccgga atgaacttga tcacgccgcc caggcgtgcg
     4021 agccccagca ccagcaggat caccccggcc atgaaagtgg cgatctgcag gccgtcgatg
     4081 ccgtgttcgg cggtaatcgc gaacaggatg acgataaagg cgccggtcgg cccggcgatc
     4141 tgtacccggc tgcccccggc gatggaaacg atgaggcccg cgatgatcgc ggtgtacagc
     4201 ccctgctcgg gctttgcgcc cgaggcaatg gcgaaggcca tcgccagcgg caacgcgacc
     4261 acggccacga tcacgcccgc gacgatgttc tgcatccagt gctcccgtcg caataaaccg
     4321 gctcgatggg cttcgacgag agcaatcatg atgtgcgggc gacctcctgt ttatatgtgc
     4381 agcgtattgt aatacgacgc acatccgccg ccgaaccggg catgcatgcc cgggcgacgc
     4441 tccgggccgc gccggagccc gccggcgtcc ggccggcgcg tcgccggcac gtcaacattt
     4501 tttacagttc tccgtcagag ctcagctatc catatgattc ttagcgattt ccttgcatgg
     4561 catagacagt gcactacgcc gggttgccaa ccggccagcc cttccacagc aaaggaggaa
     4621 atcggaatga cgcttcaatc cacgttcgca tcgctatgcg gcgcagcgct catcgcggca
     4681 ggcggcctgt acgccggccc tgcacaggcc gttcccgtcg cactcgaact cgcgctgctg
     4741 gtcgacgtat cgggcagcat cgacggcgac gaatacgacc tgcagaagca gggctacatc
     4801 aacgccttcc gcaacgccaa cgtgcagaac gcaatcgccg ggcaaagcgg cggcatcgcg
     4861 gtcacctata tcgagtggtc ggggtcgggc cagcaggccc aactcgtcgg ctggacgcat
     4921 ctcacggacg ccgcatccgc cgacgccttc gccgatgcgc tgggcctcac ctcacgcgct
     4981 ttcagtggcc agaccaatcc cgatgcggcg atcaactacg ccgttccgct gttcgacgac
     5041 aacgacttcg agggactgcg actggtgatc gacgtctccg gcgatggcac cggaaatgcc
     5101 aataacaccg cagctgcccg tgacgccgcg ctggcggcgg gcatcaacgc catcaacggc
     5161 ctggccatcg gtgggacggg cctggaagac tggtacaacg attacctgcg cggcggcgcc
     5221 ggttcgtttg tcatcggcgt tgcggacttc gaagacttcg aaggcgcggt catgaccaaa
     5281 ctgcagcgcg aaatcaccgg ctccgtgccc gaacccggca cactgatgct gctcggtttg
     5341 gccaccgccg cgctggcgtt ctcgcggcgc cggatgggct gatcaggaaa agcgcggaac
     5401 acggagaaca cggagagcac agagaaaacc caacggagtt tttgcttttc tccgtgtcct
     5461 ccgtgcttct ctgtgacctc cgtgtttcag gatttatacg tgcccgcact acacaattgt
     5521 ccgcgagacc cggcagcccg gcagccgtct tttgagcttt accctcgact ctctgctcca
     5581 gactctcgac cggctcgctt caacaccagc gcctgctccg cgtccaggtt gccgtcgtac
     5641 gacgcgccct ccccttcgcc ttcgctcacc acttcgatga gccaccggcc cgccagcggg
     5701 aactgctgcg ggcggccgct gaaattgacg cagacgacgc gttcgtcctc gccgcagcgg
     5761 cgccgatagg ccagcacttc cagcggcgac gacaactcct cccagtcgcc gtaacgcagc
     5821 gccgtgctcg ccttgcgcgc cgccagcaga cgacggtaga ggtgcaggat ggagtgttcg
     5881 tcctgccact gcgtctccgc ggcgtgcgtg gaagggtgcg gcgggaaagg cagccagggc
     5941 ttctcgccgc tccagccatg aggcggaatg ctctcccacg ggatcggcgc acgcgggccg
     6001 tcgcggccgc cgggatcgac ctgctcgtcg gggccgacct gcgcatcctc caaccccagc
     6061 tcctcgccct gataaagaaa gatggtgccg cgcagggtca gcagcatgac tgccgccgcg
     6121 cgggcgcggc gcatcgaacc gccgaagctg gtgcggtgac gcgggaaatc gtgattcgac
     6181 agtacccagg tcggccatgc gcctttagga cccagcaggc cttcgacctc gcgtatgcga
     6241 tcgcgcgcgc cgaccgcgtc ccagggcgtg tccaggtgcg agaagttgaa cgccatgtgc
     6301 aggccgtcct gctcgccgta gtagcgcaac accgcctcgg tcgagcgcac gaagatttcg
     6361 ccgaccagca cgcagtggct gggatgcgcg tcggcgaact tgcgcagacc gcggcagact
     6421 tcgtggctgt aaggctgatc gttgtgcagc atcagcggct gaccgaccgc gcaatgcgga
     6481 tcgtcgttga aggtcgggtc cttgccgatg caatgcaccg cgtcgatgcg gaatccgtcc
     6541 acgccgcggt cgagccagaa attgagtacg ccgtacatcg caccgatcac ttcgggatga
     6601 cgccagttca ggtccggctg ttccggcaga aaaagatgca ggtagtactg cccggtgcgc
     6661 tcgtccagcg tccacgcact gcccgcgccg aaggcggcga cccagttgtt ggggcggtcg
     6721 cgccagatgt accagtcgcg tttggggttg tcgcgcgagg agcgtgactc gatgaaccac
     6781 ggatgctggt ccgaggtgtg gttgggcacg tagtcgagca ggatgcgcat gccccgcgca
     6841 tgcgtctcgg cgatgagccg gtccatgtcg gccagcgtac cgaacagcgg gtcgatgtcg
     6901 cagtggtcgc tgacgtcgta gccggcatcg accatcggtg aacggtacgc cggcgacagc
     6961 cagatggcgt ccacccccag gcgttggatg tgatcgaggc gttgacggat gccggccagg
     7021 tcgccgatgc cgttgccgtc cgaatcggcg aaggaacgcg gatagatctg atacacgacc
     7081 gcgccgcgcc accagggtgc gacgacctgc tctgctgctt gcgacatgag gcctcgattg
     7141 cgagaattcg ctacaagtag caagcgcggt gccgatcgtc aatgtgggga acggcgcttg
     7201 ctcctgcttg gcggacggca tcgtgcatgc cggcccgtcg ttacgacgag gaacactcat
     7261 gtttgaagcg cttccccgtg atgcctggcg cgcctaccgc gaccaggact tccccacccg
     7321 aagctggact gccgaagcgg atgtcatccg ggcgcttgcc gagggcccac gcgtccatct
     7381 catttcacgt aagcggtacg gcgatttcga attgcatttc gactggtgcg tggccgaggg
     7441 tggcaattcc ggcgtgctct accgcgtcag cgaagactac gaagagccct ggcacagcgg
     7501 tctcgagatg caactgctcg acgatccgcg ccaccccgat ggcatgaatc cgaccaccgc
     7561 ctgcggcgcg ctttatgatc tgctgcccag ttcactcatg gaacccgtgc cgccgtcgat
     7621 gttcgtcacc gcctgcatcg tcgtgcgcgg cactgcggtc gagcactggc tcgcgaacca
     7681 gctggtgttg agctacgacc tgagcgatcc gacgttccgc cgccatgtgg cgcagagcaa
     7741 gttcaaggac taccccgcct tcggcacggt gccggaagga cacatcgtgc tgcagcacca
     7801 cggcaccggc gcggcgttcc gcaatctgcg catacgcgag ctggattagg aactagggtt
     7861 ccggccgcag ccatagccac aacgcaaggc cgcacagaaa aaccggcacg ccgatcttca
     7921 cccacaccgg cacgggcgaa tacagcactg cgatcacgct catcgacatg agcgccgacg
     7981 ccagccactt cgcacggcgc ggcaccgtgc cgcgttcgcg ccaaccgcgc agcgccggtc
     8041 cgaagcgttc gtgattcagc agccatgctt cgagttgcgg ccagccgcgg ctcgccgccc
     8101 aggcggcaac gatcagaaag ggaacggtcg gcagcaccgg caacaccgcc ccgaccagtc
     8161 cgaaggccag tgcgacaagc gccagcgcgc gccagaacag gatgcggagc atgcgcccgc
     8221 cctacttcat gcccagacgg cgcgcgagct tgtgcagatt gctcgcatcc acgccgagca
     8281 cgcgcgccgc gctcgcccag ttgtcgttgc agcgcgccag tgcggcacgg atgcattgac
     8341 gctgcgcggt ctccaccgct tcgcgcagac tgcggggccc ctgcgcgtcg tccgtcgcgg
     8401 gcaggcgcgc cgcgctgacc agttgtgcca gcggcagcat ggcttcctgt tgcgtatcga
     8461 ggtcgagcag ttccggttcg aggctgacga tttccgtgcg ttccgcgccg cggctcaccg
     8521 ccttgagcgc cgcgcggctg atcacgtgtt ccagttcgcg cacattgccc ggccattgat
     8581 aagcgcaaag cgcctgctcg gcctcgggcg tcaggcgcag actgcgcaag ccgaggcgcg
     8641 cgcggttcag ttccagaaag tgcccggcga gcaccagcac gtcgctgccg cgttcgcgca
     8701 gcggcgggat gtgcaccgga tagaccgaca gacggtgata caggtccgcg cggaaatgac
     8761 cgtcgcgcac gccgtcgcgc aactggcgat tggtcgcggc gatgatgcgc acatccaccc
     8821 ggcggggtgc atcggcgccg aggcgctgga tttcgccgtt ctgcagcgtg cgcaacagct
     8881 tcgcctgga
//
LOCUS       DATGOU010000058        16103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_5910365, whole genome shotgun sequence.
ACCESSION   DATGOU010000058 DATGOU010000000
VERSION     DATGOU010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16103
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5910365"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VKZ48_03300"
     CDS             <1..224
                     /locus_tag="VKZ48_03300"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HLU76915.1"
                     /translation="RPGQQNYDVVFLDPPYDSDLLGPALRALPVHLADDARIYVESAT
                     SVAFEPPWEVLKSGRAGAAHYYLLRLGSS"
     gene            221..709
                     /gene="coaD"
                     /locus_tag="VKZ48_03305"
     CDS             221..709
                     /gene="coaD"
                     /locus_tag="VKZ48_03305"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HLU76916.1"
                     /translation="MNKAIYPGTFDPITRGHEDLVRRAAQLFRCVVVGVADSAGKRPF
                     FTQQERVQMAREVLAAYPNVEVKGFSGLLRDFVREHKGHVILRGLRAVSDFEYEFQMA
                     GMNRMLIPEAETVFLTPGEQYQFISATMVREIAVLGGDASPFVHPIVDARLKEKVQSL
                     GG"
     gene            complement(706..2655)
                     /locus_tag="VKZ48_03310"
     CDS             complement(706..2655)
                     /locus_tag="VKZ48_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HLU76917.1"
                     /translation="MNGGLAQRFNDYSAWRDSVASRAVAYRKWLEDNELADAQTEAQI
                     DQLLARLAEDKLTIAFVAEFSRGKSELINALFFSDYGRRLLPSSAGRTTMCPTELHWD
                     PSIPPSIRLLPIETRAEASDGDLQRDSDQWVSIPLELSDTEAMVAAFARVRETKQVSA
                     AEARSYGLCAEDGLNAPTESADDHVEVPAWRYAVINFPHPLLQQGVVILDTPGLNAIG
                     TEPELTINLLPNAHAVVFVLACDSGVTQSDAEVWERHLAVAPGRHSTRMVVLNKIDAL
                     WDGLRSPADIDSEIDKQMRYAGEVLRLPRDRIFATSAQKGLLAKITNNETLLRRSRLA
                     HLERRLADDLISRKHQIVRESTLYDVSDLLVGTRTLLETRLYGVQEQLKELRGLRGRN
                     LDVVEDMMHKVREEKEDFEQALRRFQALRNVYSQQTNRLFTSLGMEALNEETKRALTA
                     LRERWFSPGLKAAMLSYFTAVREKLNSADKIIREIQRMMDAMYRQFSVEHGLQLSAPV
                     PFSAYRYLKEFERLEAEFQAQFDSPLVMLTHEKMALTQKFFGTVARQMQRMYQIANRD
                     VDAWLRAVMSPLEKQIREHQTQLRHRLESIKRIHRATDQLEDRVSELEMQEEQLMEQL
                     EALRHIAADMERALDAVDTPLAAAA"
     gene            complement(2759..3586)
                     /gene="mutM"
                     /locus_tag="VKZ48_03315"
     CDS             complement(2759..3586)
                     /gene="mutM"
                     /locus_tag="VKZ48_03315"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HLU76918.1"
                     /translation="MPELPEVEITRLGIAPHITGRVVTGVVIRHPQLRWPVPQRLGAL
                     LTGQAVQSVDRRAKYLLLAFAHGTLILHLGMSGSLRIIDAVEPAGVHDHFDLVFGATA
                     MRLRDPRRFGAVLWCAEPTVATHPLLAHLGAEPLSDAFTADLLYLATRGRKQAIKLVL
                     MDHRVVVGVGNIYANESLFRAGIRPTMAAGRLTRAAAARLVDAVRATLNDALAAGGSS
                     LRDFIHTDGSAGYFQQHYFVYGREGDNCRRCDTPIRRIVQAQRATFYCSRCQPSRPA"
     gene            3735..5462
                     /locus_tag="VKZ48_03320"
     CDS             3735..5462
                     /locus_tag="VKZ48_03320"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLU76919.1"
                     /translation="MALGGCAHLGGGAAAGAKPADSEPIVKTTDVPTAELEAIASPEL
                     PRQELTSDLLYDFLLAEIAAQRGNANVSAQTYVDLADRTKDPRIARRAHDIALAARAP
                     VLAIRAAEIWLEADPKSLDARRRLAALLLATHQLEAARPHLEHLLAAGAVDRAEVFTQ
                     LTRLIAGNPNREANLRIVQDLAAANADLPEAHFAVAHAAGQAQNYELALKSIREALRL
                     KPDWETGVLLEAQILQKSSGNDAARARLAEYLQQHPDALDIRLNHARLLVADRQYEAA
                     RDEFEGMLRDNPLNGEALYASALLALQLKDYPAAESRLKRLLELNYRDRDTVTLYLGQ
                     IAEEQKRYAEALDWYEDIIAGEHYLTAKTRQAGVLAKQGDIAGARKFLQQIKVPSNQQ
                     RVQLILAEAHLLRDAGQYQEAFGVLSRGLEKLPNDPDLLYDQAMIAEKLNRLDVLESS
                     LRKLIRLRPDHPHAYNALGYSFADRNMRLAEARKLIEKAHELAPDDAFILDSMGWVLF
                     RQGDHEQAEQYLRRAHMLRPDPEIAAHLGEVLWVAGRHDEARQIWQAAKERAPDNEAL
                     LQTLKRLRP"
     gene            5476..6027
                     /gene="lolB"
                     /locus_tag="VKZ48_03325"
     CDS             5476..6027
                     /gene="lolB"
                     /locus_tag="VKZ48_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HLU76920.1"
                     /translation="MLMLRRLTVLGLGALLAACASMPSHEGLEAGSFHVTGRVAVRYG
                     DEAATGRVAWRHTRETDDLVIANPIGQGIAQIERRDDRYVLQTQEGERFEAADPEALT
                     EQVLGWRLPLTGLPQWIGGQPMPGVPAQMDHEGGRLRTLQQMGWLIEYQAWDEASGLP
                     QRLQLSRDDLRIRLAIEAWRTGQ"
     gene            6042..6866
                     /gene="ispE"
                     /locus_tag="VKZ48_03330"
     CDS             6042..6866
                     /gene="ispE"
                     /locus_tag="VKZ48_03330"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011296533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HLU76921.1"
                     /translation="MQRYPAPAKLNLFLHVVGRRADGYHLLQSVFRLIDLCDWIGVEA
                     REDGRIVRVNDVPGVPAEADLVVRAAQRLREHAGVAHGANIVVDKNIPVGGGLGGGSS
                     DAATTLMALNHLWGLGLPREELAFLGLQLGADVPFFLGGGNAWVEGIGDVLTPISLPP
                     AWYVVISPGVAVQTAQIFAAPELTRDSKTIKITSFFTGLGRNDLEPVVKKRFPQVAQA
                     LDWLQGFGEARMSGSGSSVFAAFDSETRARAVVEQMPREWRGFAVRGLDRHPFPQQ"
     gene            6877..6953
                     /locus_tag="VKZ48_03335"
     tRNA            6877..6953
                     /locus_tag="VKZ48_03335"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6911..6913,aa:Gln,seq:ttg)
     gene            7069..8019
                     /locus_tag="VKZ48_03340"
     CDS             7069..8019
                     /locus_tag="VKZ48_03340"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HLU76922.1"
                     /translation="MAYESLMVFTGNANPLLAHNVVRRLNIPIGHAQVSKFADGEVMV
                     EILENVRGKDCFVLQSTCAPTNDNLMEVLIMIDALRRSSAGRITAAIPYFGYGRQDRR
                     ARSARVPISAKVVANLLTAVGVDRVLTMDLHADQIQGFFDVPVDNVYASPVLLGDVWK
                     HNYENLLVVSPDVGGVVRARALAKQLESDLAIIDKRRPKPNVSEVMNIIGEVEGRTCI
                     IMDDMVDTANTLCRAADALKATGAVKVVAYCTHPVLSGSAVQRIEQSSIDELVVTDTI
                     PLRADAQACKKIRVLSVAGLLAETILRISNEDSVSSLFIE"
     gene            8096..8728
                     /locus_tag="VKZ48_03345"
     CDS             8096..8728
                     /locus_tag="VKZ48_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HLU76923.1"
                     /translation="MEVKAQVRKAQGTGASRRLRRAGKTPGILYGGDEPAVAIELDHN
                     ELFQHLRKEAFHASILSLNVEGKKQPVLLRTYTMHPFRREVQHIDFQRVAADQKVRME
                     VPLHFMNQENNDAVKLGGAKITHVLTELEVRCLPSALPEYIEVDLTHIPVNGTIHAQD
                     IKLPEGVELANRGENPAVAIANVPRGAGEEGEAAAPEAAAAAAPAEEEKK"
     gene            8810..9391
                     /gene="pth"
                     /locus_tag="VKZ48_03350"
     CDS             8810..9391
                     /gene="pth"
                     /locus_tag="VKZ48_03350"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HLU76924.1"
                     /translation="MAIRLIVGLGNPGREHERDRHNVGFWFIERLADKLRVTLSKDAK
                     YHAFVTRVARPGGDVWLMMPQTYMNLSGKAVGALARFYKIAPEEILIVHDELDFPPGT
                     VKLKRGGGAGGHNGLRDIEAQLGSQATWRLRVGIGHPGDKNVVPDYVLKAPPPADREA
                     IDQALDRCLEISDLLLAGDMSAAMLKLHTKVSS"
     gene            9388..10479
                     /gene="ychF"
                     /locus_tag="VKZ48_03355"
     CDS             9388..10479
                     /gene="ychF"
                     /locus_tag="VKZ48_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006477050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HLU76925.1"
                     /translation="MTLRCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNTGIV
                     EVPDPRLAQLAEIVKPQRTVPATVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIAH
                     VVRCFEDPNVVHVAGKVDPIADIETINTELALADLQTVEKQLQKHSKVAKSGDKDAQK
                     LVAVLEKLQVALNEAKPARTVDLSKEEQALVRQFFLLTMKPTMYVANVAENGFENNPL
                     LERVEAYAKEENAPVVAISAAIEAQLADLDEEEKQLFLDDMGLDEPGLNRLIRAGYKL
                     LGLQTYFTAGVKEVRAWTIHAGDTAPQAAGVIHTDFEKGFIRAQTISFEDFIKYKGEQ
                     GAKEAGKLRAEGKEYVVQDGDVLNFLFNV"
     gene            10549..11154
                     /gene="gstA"
                     /locus_tag="VKZ48_03360"
     CDS             10549..11154
                     /gene="gstA"
                     /locus_tag="VKZ48_03360"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HLU76926.1"
                     /translation="MKLYYKRGTCALATHIALLEIGVPFETEKVDLATKKTASGGDYY
                     AVSPNGYVPALEFDGQVLTEIVSVLLYIADLKPEARLAPPKDAFERYRLLQWLGFINS
                     EVHKTYTAYFAPGATDAEKQRASEKLAKRIDFVERSLGDRPYLLGEQFTVADCYLYTV
                     LGWASVAKLDLARWPKVQAYFQRIDQRPAVRKAHEDEKAAA"
     gene            11151..11363
                     /locus_tag="VKZ48_03365"
     CDS             11151..11363
                     /locus_tag="VKZ48_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012366505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HLU76927.1"
                     /translation="MTEDEARRIATHVHYKGGLYRYIGTATHSETLEPLVVYEQLWPK
                     ARSLWVRPAAMFNETLPDGQPRFRPL"
     gene            complement(11381..12289)
                     /locus_tag="VKZ48_03370"
     CDS             complement(11381..12289)
                     /locus_tag="VKZ48_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HLU76928.1"
                     /translation="MDQLLAIRVFTRVVEAGNFTKAADSLQMPKATVTKLVQSLEAHL
                     RTTLLQRTTRRVTVTAEGAAYYERVVRLLSELDDLEQSLTHAQAKPRGRLRVDVHAAL
                     ARLLIVPALPRFLERYPDIQIDLGISDRRVDLIESNVDCVIRGGELKDSSLIARRLSG
                     LSWCTCAAPAYIARYGTPRTPADLETRHRVVNYFSSQNGRLLPFRYRRKGSDQPMIEI
                     EGQRVVGVNDSNAHLAAGLAGLGVIQSLRFSARPHIERGELVELLTDWERDPLPMHIL
                     YPPNRHLSNKVRVFIDWIAELFEATP"
     gene            12417..13166
                     /locus_tag="VKZ48_03375"
     CDS             12417..13166
                     /locus_tag="VKZ48_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007587284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLU76929.1"
                     /translation="MTKRLSNKVALVTGGSRGIGAAIARQLADDGADVVLSYVNSADK
                     AKALVRELQDKGVRAAAFRADQASAVEVRGLVEQTLEAFGRLDILVNSAGVFVTGTPG
                     DPANDEAALERQYAINVGGVVAAVHAAAPHLPPGGRIINIGSTLGTRTPFAGLADYSA
                     GKAAVAAYTRGWARDLGSKGITVNTVQPGPIDTDMNPADGDFADTARAAVALGRYGKP
                     EEIAAVVAFLAGPDASYVTGASIDVDGGFNA"
     gene            13255..13827
                     /locus_tag="VKZ48_03380"
     CDS             13255..13827
                     /locus_tag="VKZ48_03380"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009463313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HLU76930.1"
                     /translation="MKDLAYGSTVPTSAALQDDGATAHLPGMRLPSVPLAATNGHIVD
                     LSTLRGRTVVYAYPRTGVPGQSSPEGWDRIPGARGCTPQNIGFRDRYAELIAYGAAQV
                     YGLSAQDTAYQCEAVERLQLPFPLLSDEQLELARQLQLPTFHIAGMTLLKRLALIIDD
                     GVVTKVFYPVPEPDRNAAEVTVWLAQHPVA"
     gene            13862..14218
                     /locus_tag="VKZ48_03385"
     CDS             13862..14218
                     /locus_tag="VKZ48_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HLU76931.1"
                     /translation="MYVDGFVVPVPQKNLDAYRRLARKAGKVWREYGALQYVECVADD
                     VKRGKQTSFPQSVKLKPGEVVCFSWIVYKSRRQRDQVNKKVMADPRLAAYMDPKSLPF
                     DAKRMFWGGFKRLVEL"
     gene            14501..14707
                     /locus_tag="VKZ48_03390"
     CDS             14501..14707
                     /locus_tag="VKZ48_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HLU76932.1"
                     /translation="MAIGTVKWFNDQKGFGFISPEGGGDDLFAHFSAITGSGFKTLKE
                     NQRVEFEAVRGPKGMQASNIRVIE"
     gene            14906..15643
                     /locus_tag="VKZ48_03395"
     CDS             14906..15643
                     /locus_tag="VKZ48_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004567244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLU76933.1"
                     /translation="MSTTSRTAIVTGGSRGIGEAIAVRLGRDGFAVVVNYSSNSAKAQ
                     AVVRRVREQGGRAIAVQGDVAEAPDVAALFEEAQRHFGGVDVVVNSAGIMHNAPIAEG
                     SLDEFDKLIRTNLRGTYVVFAEAAKMVRRGGRIIGLSTSVIAKAFPTYGAYIAAKAGV
                     EGLVRVLANELRGREITVNAVAPGPVATDLFLHGKTEAQIEQIARMPPLERLGTPDDI
                     AGVVSFLAGPDGGWVNAQILRANGGFA"
     gene            complement(15670..>16103)
                     /locus_tag="VKZ48_03400"
     CDS             complement(15670..>16103)
                     /locus_tag="VKZ48_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HLU76934.1"
                     /translation="MTLTAPIRLVTEAAQETPHGPYRLTESGGEYVLNAQLDDEWKPL
                     YAFNLEEHTPPDYELMNWYTSTHPRSLFVTDLIAARAMREGRHTLRNNRYGFRGLSGV
                     KGEQRIRTVAELKALLSSVFGIEVPNVPHADERFARALEAD"
BASE COUNT         2940 a         4988 c         5302 g         2873 t
ORIGIN      
        1 tgcgccccgg gcagcagaac tatgacgtgg tctttctcga tccgccctat gacagcgatc
       61 tgctcgggcc cgcactgcgc gcgctgccgg tccatctggc ggatgacgcg agaatctatg
      121 ttgaatcggc gacctccgtc gcgttcgagc ctccctggga ggtgttgaag tcggggcgag
      181 ccggcgcagc gcactactat ctgttgcggc tggggagttc atgaacaaag cgatctatcc
      241 ggggactttc gatcccatca cacgcgggca tgaggacctg gtgcgccgtg ccgcgcaact
      301 gttccgctgc gtcgtggtcg gcgttgccga cagtgccggc aagcggcctt tcttcacgca
      361 gcaggaacgg gtacagatgg cccgcgaagt gctcgccgcc tatcccaacg tcgaggtgaa
      421 gggtttctcc ggtctactgc gcgatttcgt gcgcgaacac aagggacacg tcattctgcg
      481 cggcctgcgg gcggtgtcgg atttcgaata cgagttccag atggcgggca tgaaccgcat
      541 gctcatcccc gaggcggaaa cggtcttcct gacgccgggc gagcagtacc agttcatctc
      601 ggcgaccatg gtgcgggaaa ttgccgtgct gggcggggac gcttcaccct tcgtgcatcc
      661 gatcgtggat gcccggctga aggaaaaagt gcagtcgctg ggcggttagg ccgccgcggc
      721 cagcggcgta tcgacggcgt ccagcgcccg ctccatgtcg gcggcgatgt gccgcagcgc
      781 ctccaactgc tccatcagct gttcttcctg catctccaac tccgagacgc ggtcttccaa
      841 ctggtcggtc gcgcggtgga tgcgcttgat cgattccaga cgatggcgca actgcgtctg
      901 atgttcgcgg atctgctttt ccagcggtga catcaccgcg cgcagccagg cgtcgacgtc
      961 gcggttggcg atctggtaca tgcgctgcat ctggcgcgcc accgtgccga agaacttctg
     1021 cgtcagcgcc atcttctcgt gggtgagcat gaccagcggg ctgtcgaact gggcctgaaa
     1081 ctcggcttcg agccgctcga attccttcag gtagcgatag gcggagaacg ggacgggcgc
     1141 gctgagctgc aggccgtgct cgaccgagaa ctgccggtac atcgcgtcca tcatgcgctg
     1201 gatttcgcgg atgatcttgt cggcgctgtt gagcttttcg cgcaccgcgg tgaagtagga
     1261 cagcatcgcg gccttgaggc ccgggctgaa ccagcgttca cgcagcgccg tgagcgcgcg
     1321 cttggtctcc tcgttcaacg cctccatgcc gagcgaggtg aacaggcggt tggtctgctg
     1381 cgaatagacg ttgcgcaagg cctggaagcg gcgcagcgcc tgttcgaagt cctccttctc
     1441 ctcgcgcacc ttgtgcatca tgtcctcgac cacatcaagg ttgcggccgc gcagcccgcg
     1501 caattccttc aactgctcct gtacgccgta cagccgcgtc tccagcagcg tgcgcgtgcc
     1561 gaccagcagg tcggacacgt cataaagcgt ggactcgcgc acgatctgat gcttgcgcga
     1621 aatgaggtcg tcggcgagac ggcgttccag gtgcgccagt cggctgcggc gcagcagcgt
     1681 ctcgttattg gtgatcttgg cgagcagtcc cttctgcgcg gaggtcgcga aaatccggtc
     1741 gcgcggcagg cgcagcactt cgcccgcata gcgcatctgc ttgtcgattt cgctgtcgat
     1801 gtccgccggc gaacgcagtc cgtcccacag tgcgtcgatc ttgttcaaca ccaccatgcg
     1861 cgtcgaatgg cggccgggcg ccactgccag atggcgctcc cagacttccg catcggattg
     1921 cgtcacgccc gaatcgcagg cgagcacgaa tacgaccgcg tgtgcattgg gcagcaggtt
     1981 gatcgtgagt tcgggctccg taccgatggc attgaggccg ggcgtatcga gaatcaccac
     2041 cccctgctgc agcagggggt gcgggaagtt gatgaccgcg tagcgccagg cgggcacttc
     2101 gacgtgatcg tcggccgatt cggtcggtgc attgaggccg tcttcggcgc acaggccgta
     2161 gctgcgcgcc tcggcggcgg aaacctgttt ggtttcgcgc acccgtgcaa aggcggcgac
     2221 catggcctcg gtgtcggaca gttcgagcgg gatgctcacc cactggtcgc tgtcgcgctg
     2281 cagatcgccg tcggaggctt cggcgcgcgt ttcaatgggc agcagccgga tggacggcgg
     2341 tatcgacggg tcccagtgca actccgtggg acacatggtc gtgcggccgg ccgacgaggg
     2401 cagcagacga cggccatagt cggaaaagaa cagggcgttg atgagttccg atttgccgcg
     2461 cgagaactcg gcgacgaaag cgatggtgag cttgtcctcg gccaggcggg cgagcaactg
     2521 gtcgatttgc gcttccgttt gcgcgtcggc cagttcgttg tcttccagcc atttgcgata
     2581 agccactgcg cgactggcga cactgtcgcg ccatgcgctg tagtcgttga agcgttgcgc
     2641 caaaccgccg ttcatgccag acgtcctcga gtcgtactgc cgttcggttt ttcgatttat
     2701 cagcggacac tgccctgcat tgcaggcgct ggggactcgt taatctagca tgccccgatc
     2761 aggcaggccg ggacggttgg caccgggagc agtaaaaggt cgcacgttgc gcctggacga
     2821 tgcgacggat cggggtgtca catcttcggc agttgtcgcc ttcgcggccg tatacgaagt
     2881 agtgttgctg gaagtagccg gcgctgccgt ccgtatgaat gaaatcgcgc agactgctgc
     2941 cgccggcggc aagcgcgtcg ttcaaggttg cgcggacggc gtcgaccagt cttgcggccg
     3001 ccgcgcgcgt gaggcggccg gccgccatgg tggggcgaat gccggcgcga aaaagacttt
     3061 cgttggcgta gatgttgcct acgcccacca ccacgcgatg atccatcagc acaagcttga
     3121 tcgcctgctt gcgtccgcgc gtcgccagat agagcaaatc ggcggtgaac gcatcggaca
     3181 acggttccgc acccagatgg gcgagcagcg gatgcgtggc gaccgtcggc tcggcgcacc
     3241 agagtacggc gccgaagcgg cgcggatcgc gcaggcgcat cgccgttgcg ccgaacacca
     3301 gatcgaaatg gtcgtgcacg ccggccggct cgacggcgtc tatgatgcgc aagctgcccg
     3361 acatgcccaa atgcaggatg agcgtgccgt gtgcaaaagc cagcagcaga tatttcgcgc
     3421 gccggtccac cgattgcacg gcttgcccgg taagcagcgc gccgagtcgt tgcggaacgg
     3481 gccagcgcag ttgcgggtgg cggatgacga cgccggtgac gacgcggccc gtgatgtgcg
     3541 gcgcgatgcc cagacgggtg atttcgactt cgggtaactc aggcacgatg cggaagcgga
     3601 cggatagcgg gcgccggttc ggggtccgac cgtggaaata tactaaactg ccgtttccgc
     3661 ctcgccttac gacgcttcgc atgacttccc ttcacgcttt tttccggccg gccgcggctg
     3721 ctgtcgcggt tgttttggcg ctcggcggtt gcgcgcatct gggcggcggg gcggccgccg
     3781 gagcaaagcc tgcggacagc gagccgatcg taaaaacgac ggatgtgccg acggcggagt
     3841 tggaggcgat tgccagcccc gaattgccgc gacaggagtt gacgagcgac ctgctgtacg
     3901 acttcctgct cgccgagatc gccgcgcagc gcggcaacgc gaacgtgtcg gcgcagacct
     3961 atgtcgatct cgccgaccgc accaaagacc cgcgcatcgc gcggcgcgcg cacgacatcg
     4021 cattggcggc gcgcgcgccc gtgctggcca ttcgtgcggc ggagatctgg ctggaggccg
     4081 atcccaaatc gctcgacgcg cgccgtcggc tggcggccct gctgctcgcc acgcaccaac
     4141 tcgaagccgc gcgcccgcat ctggaacatc tgctggcggc cggtgcggtg gatcgtgcgg
     4201 aagtcttcac ccaactgacc cgtctgatcg ccggcaatcc caaccgggaa gccaatctgc
     4261 gcatcgtgca ggatctcgca gccgcgaatg ccgatctgcc cgaagcgcac tttgccgtgg
     4321 cgcatgcggc cggacaggcg cagaactacg aactcgcgtt gaaatccatc cgggaagccc
     4381 tgcgcctcaa gcccgactgg gaaacgggtg tgcttctgga agcgcagatc ctgcagaaat
     4441 cgtcgggcaa cgatgcggcc cgcgcgcggc ttgcggagta tctgcagcag catccggatg
     4501 cgctcgatat ccgcctcaat catgcgcgcc tgctcgtcgc ggatcgccaa tatgaagcgg
     4561 cgcgcgacga attcgagggc atgttgcgcg acaaccctct caacggcgag gccctttacg
     4621 cgtccgcgct gctggcgctg cagttgaagg actatccggc cgccgagagc cgcctcaagc
     4681 gcctgctcga actcaactat cgcgatcgcg acaccgtgac gctttatctc gggcagatcg
     4741 ccgaggagca gaagcgttat gccgaggcgc tcgactggta cgaggacatt atcgcgggcg
     4801 agcactacct gaccgccaag acgcgccagg cgggcgtgct cgccaaacag ggtgatatcg
     4861 cgggcgcgcg caaattcctg caacagatca aggtgccgag caaccagcag cgagtgcagc
     4921 tcattcttgc cgaagcgcat ctgctgcgcg acgcggggca gtatcaggaa gcattcggcg
     4981 tgttgtcgcg cggactcgag aagctgccca acgatccgga cctgctctac gaccaggcca
     5041 tgatcgccga gaagctcaac cgcctggacg tgctggagtc ctcgctgcgc aagctgatcc
     5101 gcctgcgccc ggatcatccg catgcctaca acgcgctcgg ctattccttc gccgaccgca
     5161 acatgcgtct cgcggaagcg cgcaagctga tcgaaaaagc gcatgagctg gcacctgatg
     5221 atgcgttcat cctcgacagc atgggctggg tgctgtttcg ccagggcgac catgagcagg
     5281 ccgagcagta cctgcgccgc gcgcacatgc tgcgccccga cccggagatt gccgcccatc
     5341 tgggcgaggt gttgtgggtc gccggccggc acgacgaggc gcgccagatc tggcaggccg
     5401 caaaagagcg cgcaccggac aacgaggcgt tgctgcagac cttgaagcgt ctgcggccct
     5461 gagggatcag gcgggatgct gatgctgcgt cgtctgacgg tcctgggttt gggcgcgctg
     5521 ctcgccgcgt gcgcgagcat gcccagtcac gaagggctcg aggccggcag tttccacgtc
     5581 accggccgcg tcgcggtgcg ctatggcgac gaggccgcga cggggcgcgt ggcatggcga
     5641 catacccgcg agaccgacga tctcgttatt gccaacccga tcggacaggg catcgcgcaa
     5701 atcgagcggc gcgacgatcg ctatgtactg caaacgcagg agggtgagcg cttcgaggcg
     5761 gccgaccccg aagcgctcac cgagcaggtg ctgggttggc gtctgccgct caccgggctg
     5821 ccgcaatgga tcggcggcca gccgatgccc ggtgtgccgg cgcaaatgga tcacgaaggc
     5881 ggacgcctcc gtaccctcca gcagatgggc tggctgatcg aatatcaggc ctgggacgaa
     5941 gcgagcggtt tgccgcagcg gctgcaactg tcgcgcgacg atctgcgtat ccgcttggcc
     6001 atcgaggcct ggcgaaccgg gcagtgactt cagttcgtta catgcagcgc tatcccgccc
     6061 ccgccaagct caatcttttc ctgcacgtcg tcggccggcg tgcggacggt tatcacctgc
     6121 tgcagagtgt cttccgcctc atcgatctgt gcgactggat cggcgtcgag gcacgcgagg
     6181 acgggcgtat cgtccgcgtc aacgacgtgc ccggtgtgcc ggccgaagcg gatctggtgg
     6241 tgcgcgcggc gcagcggtta cgcgagcacg cgggcgttgc gcatggtgcg aacatcgtcg
     6301 tcgacaagaa catccccgtg ggcggcggac tgggcggcgg cagttcggat gccgccacga
     6361 ccctgatggc cctcaaccat ctgtggggtc tcgggttgcc acgcgaagaa ctcgccttcc
     6421 tgggcctgca actgggcgcc gacgtgccct ttttcctggg cggcggcaac gcctgggtgg
     6481 aaggcatcgg ggatgtgctc acgccgatca gcctcccgcc tgcgtggtat gtggtcattt
     6541 cgcccggggt ggcggtgcag accgcacaaa tcttcgccgc gcctgaattg acacgtgata
     6601 gtaaaactat caaaatcacg tcctttttca cgggcttggg ccgtaacgac ctcgagccgg
     6661 tggtgaaaaa acgctttccg caagtggcgc aggcgctgga ctggctgcaa gggttcggcg
     6721 aggcgcgcat gagcggctcg ggatcgagcg ttttcgcggc tttcgacagt gagacgcggg
     6781 cacgggcggt cgtcgagcaa atgccgcggg agtggcgcgg attcgcggtc cggggtctgg
     6841 accggcatcc gttcccgcag caatagcccg aacaactggg gagtcgccaa gctggttaag
     6901 gcactggatt ttgattccag catgcgaagg ttcgaatcct tcctccccag ccataccggc
     6961 tgcagcgcat caagcgcatg gattgaataa ccagccgcaa gcagtgcttg cggctgttgt
     7021 tttttgcacg acagaggctc tggcggcgac ggccgcggat gatcagcgat ggcgtacgaa
     7081 agcctgatgg tctttacggg gaacgcgaat cccctgctgg cgcataacgt cgtcaggcgg
     7141 ctcaatattc cgatcggcca tgcgcaagtc agcaagttcg ccgacggcga ggtgatggtc
     7201 gagatcctcg aaaacgtgcg cggcaaggat tgcttcgtcc tgcagtccac ctgtgcgccg
     7261 accaacgaca acctcatgga agtgctgatc atgatcgacg ccctccgtcg atcgtcggcc
     7321 ggccgcatca ccgcggcgat tccgtatttc ggctacggcc gccaggatcg tcgggcgcgt
     7381 tcggcgcggg tgccgatcag cgccaaggtc gtggccaacc tgctcaccgc ggtgggcgtg
     7441 gatcgcgtgc tgaccatgga cctgcacgct gatcagatcc agggcttctt cgacgtgccg
     7501 gtggacaacg tgtacgcttc gcccgtcctg ctgggtgacg tttggaagca caactacgag
     7561 aatctgctgg tcgtatcgcc ggacgtcggc ggtgtggtgc gggcccgtgc gctggcgaag
     7621 caactggagt cggacctcgc cattatcgac aagcgccggc cgaagccgaa cgtgtccgaa
     7681 gtgatgaaca tcatcggcga agtcgagggc cgtacctgca tcatcatgga cgacatggtg
     7741 gacacggcca atacgctctg ccgcgcggcc gatgcgctca aggcgacggg cgcggtcaag
     7801 gtggtggctt actgtacgca tcccgtgctc tcgggcagtg ccgtgcagcg gatcgagcag
     7861 tcgagcatcg acgaactggt cgtgaccgac accattccac tgcgtgcgga tgcccaggcg
     7921 tgcaagaaaa tccgcgtgct gtcggttgcc ggtctgctgg ccgaaacgat tttacgtatc
     7981 agcaacgagg actccgtgtc ctcgttgttc atcgagtaag cgaggaacgc ttcgcttttt
     8041 aacaaccgcg cgggatctgg tcgcggaccc gcgaaactgg agttgcatca tgcaaatcga
     8101 agtcaaagcg caagtgcgca aagcgcaggg aacgggtgcg agccgccgtc tgcgccgcgc
     8161 cggcaagacg ccgggcatcc tctatggcgg ggacgagccc gctgtggcga tcgaactgga
     8221 ccataacgaa ctgttccagc atctgcgcaa agaggcgttc cacgcatcga ttctgagcct
     8281 gaacgtcgag ggcaagaagc agccggtgct gctgcgcacg tacaccatgc atccttttcg
     8341 ccgggaagtg cagcacatcg acttccagcg cgttgccgcc gatcagaagg tccgcatgga
     8401 agtgccgctg cacttcatga accaggagaa caacgacgcg gtgaagctgg gtggcgcgaa
     8461 gatcacgcac gtcctcaccg aactggaagt gcgctgcctg ccgagcgcgc tgccggagta
     8521 catcgaggtc gatctgaccc acattcccgt caacggcacg attcacgcgc aggacatcaa
     8581 gctgcctgag ggcgtggaac tcgccaaccg cggcgagaac ccggccgtgg ccatcgccaa
     8641 cgtgccgcgc ggcgcgggcg aggaagggga ggcggccgct ccggaagccg ctgctgcagc
     8701 tgcgcccgcc gaagaagaga agaagtaagc atctgctgca ggaaacaagg cgcgcgggcg
     8761 accgacgcgc cttttttctt tgcgcgatgc actgacagac ggaaaccgta tggccatccg
     8821 actcatcgta ggcttgggca accccgggcg cgaacatgaa cgcgaccggc acaacgtcgg
     8881 tttctggttc atcgaacggc tggccgacaa gctgcgcgtg acgctcagca aggatgcgaa
     8941 ataccacgcg ttcgtgaccc gcgtcgcccg gccgggcggc gatgtgtggc tgatgatgcc
     9001 gcagacctac atgaatctct ccggcaaggc cgtcggcgca ctcgcccggt tctacaagat
     9061 cgcccccgaa gagattctga tcgtgcacga cgaactggat tttccgcccg gcacggtgaa
     9121 gctcaagcgg ggcggcggtg cgggcggcca caacggcctg cgcgacatcg aggcgcagct
     9181 cggcagccag gccacatggc ggttgcgtgt gggcatcggt catccgggcg acaagaacgt
     9241 cgtgcccgat tacgtgctga aggcgccgcc gcctgccgac cgggaggcga tcgaccaggc
     9301 gctcgaccgc tgtctggaga tttcggattt actgctcgcc ggcgacatga gcgccgcgat
     9361 gctcaagctg catacgaagg tttcatcatg acgctacgct gcggaatcgt cggtttgccc
     9421 aacgtcggca aatccacctt gttcaacgca ctgacgaagg cgggcatcgc cgccgagaac
     9481 tacccgttct gcaccatcga gccgaacacc gggatcgtcg aagtgcccga tccgcgcctg
     9541 gcgcaattgg cggagatcgt gaaaccgcag cgcacagtgc cggcaacggt ggagttcgtc
     9601 gatatcgccg gcctggtcgc cggtgcgtcg aagggcgaag gcttgggtaa ccagttcctg
     9661 gccaacatcc gcgaaacgga tgcgatcgcg catgtggtgc gctgtttcga agacccgaac
     9721 gtggtgcacg tggcgggcaa ggtcgatccc atcgccgaca tcgaaaccat caacaccgaa
     9781 ctggcgctgg ccgacctgca gacggtcgag aagcaattgc agaagcacag caaagtcgcc
     9841 aagtcgggcg acaaggacgc gcagaagctc gtcgccgtgc tggagaagct gcaggtggcg
     9901 ctgaacgaag cgaaaccggc tcgcacggtc gatctgtcca aggaagagca ggcgctggtg
     9961 cgtcagttct tcctgctgac catgaagccc accatgtacg tcgccaacgt ggcggagaac
    10021 ggcttcgaga acaacccgct gctggagcgc gtcgaggcgt acgccaagga agagaacgcg
    10081 cctgtcgttg cgatcagcgc cgcgatcgaa gcgcaactcg ccgatctcga cgaggaagag
    10141 aagcaactgt ttttggacga tatgggcctg gacgagcccg ggctcaatcg actcatccgc
    10201 gcgggctaca agctgctcgg tctgcagacc tacttcaccg cgggcgtaaa ggaagtgcgc
    10261 gcatggacca tccatgccgg cgacacggcg ccgcaggcgg ccggcgtgat tcatacggac
    10321 ttcgagaagg gtttcatccg cgcgcagacg atctccttcg aagacttcat caaatacaag
    10381 ggcgaacaag gcgccaagga ggcgggcaag ctgcgcgccg aaggcaagga atacgtcgtg
    10441 caggacggcg acgtcctgaa tttcctgttc aacgtctgaa tcggcgcttg cacaaccggg
    10501 tcgagtgttt gcatccttga aggtcctgca accgtaaagg aggcaacgat gaaactttac
    10561 tacaagcgcg gcacctgcgc gctggccacg catatcgcct tactggaaat aggcgttccg
    10621 ttcgagaccg aaaaagtcga tctcgcaacc aagaagacgg cgagcggcgg cgattactac
    10681 gccgtcagtc cgaacggcta tgtgcctgcg ctggaattcg acggacaggt cctgaccgag
    10741 atcgtgtcgg tgctgctcta tattgccgac ctcaaacccg aagcccggct ggcgccgccc
    10801 aaggatgcgt tcgagcgtta tcgccttctg cagtggctgg ggttcatcaa ctccgaagtc
    10861 cacaagacct acaccgccta cttcgcgccg ggcgcgaccg atgcagagaa gcagcgcgcg
    10921 agcgaaaagc tggccaagcg catcgacttc gtcgagcgtt cgctgggcga ccggccgtat
    10981 cttctgggcg agcagttcac ggttgccgat tgctatctct acaccgtgct cggttgggct
    11041 tccgtcgcga agctggacct tgctcgctgg ccgaaggttc aggcctattt ccagcggatc
    11101 gatcagcggc ccgcggtgcg caaagcacac gaggatgaaa aggccgccgc gtgacggaag
    11161 acgaagcgcg ccgcattgcc acgcacgtgc attacaaggg cgggctgtac cgctacatcg
    11221 gcactgcgac gcactccgaa acgctggaac ccttggtggt ctatgagcag ctctggccca
    11281 aagcgcgttc gctttgggtg cgcccggctg cgatgttcaa cgagacgctt cccgacggac
    11341 agccccgctt ccgtccgttg taatgccgaa tcgccgccgg tcaaggcgtc gcctcgaaca
    11401 actccgcaat ccagtcgatg aacacacgca ccttgttgct gagatgccgg ttgggcggat
    11461 agagaatgtg catcggcagg ggatcgcgct cccagtcggt caacagctcc accagttcac
    11521 cgcgctcgat gtgcggacgt gcgctgaagc gcagtgattg gatgaccccc aatcccgcca
    11581 gacctgccgc tagatgcgcg ttgctgtcgt tcacgccgac gacgcgctga ccttcgatct
    11641 cgatcatcgg ttggtccgaa cccttgcgcc gataccggaa cggcagcagg cggccgttct
    11701 gcgaagaaaa gtaattcacc acccggtggc gcgtttccag gtccgccggt gtgcgcggcg
    11761 tgccgtagcg ggcgatgtaa gccggcgcgg cgcaggtgca ccagctcaaa ccgctcagcc
    11821 ggcgtgcgat gagcgaggag tccttcagtt caccgccgcg gatcacgcaa tcgacgttgc
    11881 tttcgatcag atccacgcgc cggtcgctga tgcccaggtc gatctgtatg tcgggatagc
    11941 gctcgaggaa ccgaggcaat gccggcacga tgagcagtcg cgccagcgcg gcatggacat
    12001 cgacgcggag gcggccgcgc ggtttcgcct gcgcgtgcgt gaggctctgt tcgagatcgt
    12061 ccagttcgct taaaagccgg acgacccgct cgtaataggc ggcaccctct gcggtgaccg
    12121 tgacgcgccg cgtcgtgcgt tgcaacagcg tggtgcgcag atgcgcttcg aggctctgga
    12181 cgagcttggt caccgtcgct ttgggcatct gcagcgaatc ggccgctttg gtgaagttgc
    12241 cggcctccac cacgcgagtg aacacgcgga tggcgagtag ttgatccata ggcactctcc
    12301 ggagactcgc cgcgattatt caccagcgga aatacagaag ccataaacgc cccctttatc
    12361 cgcttcggca tgagcaatag agtgcgccgc tcctacgatt catatggaga gcggtcatga
    12421 cgaaacgatt gagcaacaag gtggcgttgg tgacgggcgg ttcgcgcggt atcggggcgg
    12481 cgattgcgag acagttggct gacgacggcg cggatgtggt tctcagctac gtgaattctg
    12541 ccgacaaggc gaaagcgctg gtgcgcgaac tgcaggacaa gggcgtgcgc gccgcggcgt
    12601 tccgggccga tcaggcgagc gccgtcgaag tgcgaggact cgtagagcag acgctcgaag
    12661 ccttcggccg cctcgacatc ctcgtcaaca gcgcgggggt gttcgtgacc ggcacgccgg
    12721 gcgatccggc caatgacgaa gcggcgctcg aacgtcagta cgcgatcaat gtgggcggcg
    12781 tggtggccgc tgtgcatgca gcagcgccgc atcttcctcc gggcggtcgc atcatcaata
    12841 tcggctctac gctcggtacg cgcacaccgt tcgccggcct ggccgactac tcggcgggca
    12901 aggcggccgt cgctgcgtat acgcgcggct gggcgcgcga tttgggttcc aaaggcatta
    12961 ccgtcaacac ggtgcagccc ggtcccatcg acaccgacat gaatccggcg gacggcgact
    13021 tcgccgacac cgcacgcgct gccgtcgcgc tggggcgcta tggcaaaccg gaagagatcg
    13081 ccgccgtcgt cgcctttctt gccggcccgg atgcgtctta tgtgacaggt gcatcgatcg
    13141 acgtggacgg cggtttcaac gcttgatgcc gcgcgccgtg acgcttgtcc ggttccgggc
    13201 tgtcacggcg caagtcgctc tgctacgctg cctgcctgcc aatggggagc gggcatgaag
    13261 gaccttgcat acggatcgac ggtacccacg agcgcggcgc tgcaggacga cggcgcaacc
    13321 gctcatcttc ccgggatgcg attgccctcg gtgccgctcg cggccaccaa cggacacatc
    13381 gtcgatctgt ccactttgcg cggccggacc gtcgtgtacg cctacccgcg caccggtgtg
    13441 ccggggcagt ccagccccga gggttgggat cgcattcccg gcgcgcgcgg ctgcacgccg
    13501 cagaacatcg gttttcgcga ccgctacgcc gaactcatcg cttatggtgc agcacaggta
    13561 tacggtctgt ccgcgcagga cacggcctac cagtgcgaag cggtcgaacg cctgcaactg
    13621 ccctttccgc tgctgtccga cgagcaactc gagttggccc ggcagttgca actgccgaca
    13681 ttccatatcg caggcatgac gctgctgaag cggctcgcgt tgatcatcga cgacggcgtg
    13741 gtgaccaagg tgttctaccc tgtgcccgag ccggatcgga acgctgccga agtcaccgtc
    13801 tggcttgcgc agcatccggt cgcttagcga cccggctcgc attcgacccg aggaggaagg
    13861 catgtatgtc gacggcttcg tagtcccggt gccgcaaaag aatctggacg cctaccggcg
    13921 gctcgcgcgc aaggcgggca aggtgtggcg cgaatacggc gcgctgcagt acgtggagtg
    13981 cgtggcggac gacgtcaaac gcggcaagca gacctcgttt ccgcaaagcg tgaagctcaa
    14041 gccgggcgag gtggtctgct tttcgtggat cgtttacaag tcgcggcgcc agcgcgacca
    14101 ggtgaacaag aaagtcatgg ccgatcccag gctggcggcg tacatggatc ccaagtccct
    14161 gccgttcgac gcgaagcgca tgttctgggg cggcttcaag cggctggtcg agttatagcg
    14221 gccgcccggc ctcggccggc gcgcctaagg ccgaatcggc cgcgggaaac ggccggcaag
    14281 gcggctgttg cggcaggcgt ccgccccggc gggcacacgc acgaaaatat tgcttgacgg
    14341 gcgtaattgc ggcctgtacg gtgcgttccg ctaggtcatc aagcagcgtt agtcccgtag
    14401 tttccctcag tagaactcgt tctcccactt cttgaagcct tgcccgtatc gggcgtaagc
    14461 ccggattttt catcagcagt aatcagtcag gtataagtaa atggcaatcg gtacagtgaa
    14521 gtggttcaac gaccaaaaag gtttcggctt catcagccct gagggcggcg gcgacgatct
    14581 gttcgcgcat ttctccgcca tcaccggcag cggcttcaag acgctgaagg agaaccagcg
    14641 cgtcgagttc gaagcggttc gcggccccaa aggcatgcaa gcctcgaaca tccgcgtgat
    14701 cgaataagtc ggatcatcgc gatctcgaaa gccgccttcg ggcggctttt ttgttttctg
    14761 tctccgcatg gtcgatgcgg ttatacgcga cggggatggc agggtccgca ttgttggtcc
    14821 acgtggaatt ctctagccga accatccctc tttattcgca gcttgcgacc ggcaacaatg
    14881 cctccatccc attagggaga cagccatgag cacgacatca cggacagcca tcgtcaccgg
    14941 cggctcgcga ggaattggtg aagcgattgc cgtgcgtctg gggcgcgacg ggttcgcggt
    15001 ggtcgtcaac tactcgagca acagcgccaa agcgcaggcg gtcgtgcggc gcgtacggga
    15061 gcagggcggt cgggccatcg ccgtgcaagg cgatgtggcc gaggcgcccg acgttgccgc
    15121 gctgttcgaa gaagcgcagc gccatttcgg tggcgtcgat gtggtagtga acagtgccgg
    15181 catcatgcac aacgcgccga tcgccgaagg cagtctcgac gagttcgaca aactgatacg
    15241 caccaacttg cgcggcacct atgtcgtctt tgcggaggcg gcgaagatgg tgcgccgtgg
    15301 cggtcggatc atcggtctgt ccaccagtgt gatcgccaag gcgttcccga cttacggcgc
    15361 ttacatcgcg gccaaggcgg gcgtcgaagg actggtccga gtgctggcca atgaactgcg
    15421 cgggcgcgag atcaccgtca acgccgtggc gccggggccc gtggccaccg atctgtttct
    15481 gcacggaaag actgaggcgc agattgagca gatcgccagg atgccgccgc tcgaacgcct
    15541 cggcacgccc gacgatatcg ccggcgtcgt gtccttcctt gccgggccgg acggcggctg
    15601 ggtcaacgcg cagatcctgc gggcaaacgg cggcttcgcc tgacgttgcc ttcggggggc
    15661 agtggtggac tagtcggcct ccagcgctcg cgcaaacctt tcgtccgcat ggggcacgtt
    15721 gggcacttcg atgccgaaga cgctggagag tagcgccttc aattcggcga ccgtgcggat
    15781 ccgctgttcg cccttcacgc cgctgaggcc gcggaagccg tagcggttgt tgcgcagcgt
    15841 atgccggccc tcgcgcatcg cccgcgccgc aatcaggtcg gtcacgaaaa gcgagcgcgg
    15901 atgggtggag gtgtaccagt tcatcagctc gtaatccggc ggcgtgtgct cctcgaggtt
    15961 gaaggcgtac agcggcttcc actcgtcgtc gagttgcgcg ttcaatacgt actcgccgcc
    16021 gctttcggtg aggcggtacg ggccgtgcgg cgtctcctga gcagcctcgg tgacaagtcg
    16081 gatgggtgcg gtaagcgtca tgc
//
LOCUS       DATGOU010000059        14237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_5910952, whole genome shotgun sequence.
ACCESSION   DATGOU010000059 DATGOU010000000
VERSION     DATGOU010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14237
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5910952"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VKZ48_03405"
     CDS             complement(<1..948)
                     /locus_tag="VKZ48_03405"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HLU76935.1"
                     /translation="MIRVTARWLVCAAFFFVPAVHAQDDGAVQVETETVVVTATRAPA
                     AIQGVPASTSVVSRKELEQLKPVRLGDAVADVPGLYVRGAAMGSNFPGTGQAVLSLRG
                     IPRTPRTLVMVDGQPVNNALTGGINVVGIPVDNLERVEVVRGPYSALYGGAAMGGVVN
                     FITAGPDQPLTEVRVGAGSMRQRGATFIHRKRYEGGVGISFLMGYRASDGDPDAEPVL
                     LTPPAPFPPVPPGTPVTGARPSSTLDGKPAYWIGHKGERPWRQRIGQLNLHYSPSAAT
                     DLTVGASWADYDMGYSRPESFLRDGAGNAVFEGPVSFGGQ"
     gene            1231..2847
                     /locus_tag="VKZ48_03410"
     CDS             1231..2847
                     /locus_tag="VKZ48_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HLU76936.1"
                     /translation="MQYQGGGSPMAGEEMHHNINPKAPPMTKAEFDKGRQMYFERCAG
                     CHGVLRKGATGKPLTPDITLERGMATLKTFIKYGSPAGMPNWGTSGEMTDEEIDLMAR
                     YIQQEPPTPPEFGMAEMKKTWKVLVKPEDRPKKKLNNWDIDNLFSVTLRDTGEIALID
                     GASKKIITFIKTGYAVHISRMSASGRYLYVIGRDGKINLIDLWMETPDNVAEIRIGLE
                     ARSVETSKYKKYEDVFAVAGAYWPPQYVIMKGDTLEPLKIQSTRGTTVDTQEYHPEPR
                     VAAIVAMHDKPEFIVNVKETGKALVVDYSDLNNLKTTEIGAAPFLHDGGWDASKRYFL
                     VAANQSNKVAVIDSHDNELEALVDVGTIPHPGRGANFKHPKFGPVWATGHLGDETIAL
                     IGTDPKKHPKSAWKVVQSLNAQGGGNLFIKTHPKSSNLWVDTPLNPDPKIAQTVAVFD
                     INNLDKPYEVLPIGEWANVGEGAKRITQPEYNKAGDEVWFSVWSAKNQESAIVVVDDK
                     TRKLKTVIKDPRLITPTGKFNVYNTQHDVY"
     gene            2859..3179
                     /locus_tag="VKZ48_03415"
     CDS             2859..3179
                     /locus_tag="VKZ48_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLU76937.1"
                     /translation="MKRTTVLHLIIAAAAFGMTAPAFADAALAAKYSCTACHSPDKKM
                     LGPAYKDVAAKYQNDAEAPARLAGKVKQGSSGVWGPVPMPPNAGVPDDDVKKLVAWVL
                     AGAK"
     gene            complement(3551..4909)
                     /locus_tag="VKZ48_03420"
     CDS             complement(3551..4909)
                     /locus_tag="VKZ48_03420"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HLU76938.1"
                     /translation="MGMIAALKVFLDTLEEGVLFLDTHRRVVEINEAAMQVLGRDSGQ
                     LIERLCPTLFVGTQCARTCEVRGGCALTPSPGEARKSQDVTLERSDGSEVALRMWAML
                     LPPNDAGLYCAIVLRDRSREVALENEVRERWRLGGLVGRTPVMQSLYAQILRAAASDA
                     SALITGESGVGKELVARALHDNSPRSGGPYIPLHCGALPETLLESELFGHAKGAFSGA
                     ATTRIGRFEAAHGGTLLLDEIGEISPAMQTKLLRVLQEKEVVRLGENHARKVDVRVIA
                     ATNRDLAAMARRGEFREDLYYRLRVLPLEVPPLRERRDDIALIAGKIAGELAERYRRE
                     PMRFARGTMDALEAYDWPGNVRQLYNALEFAAVQADGPLILPRHLPPEILQPAAPAAP
                     THDRYAPPLTRYYRPVVSPDEERGIIRRVLQECGGNRAEAARRLGMSRTTLWKRLSAE
                     KG"
     gene            5083..5664
                     /locus_tag="VKZ48_03425"
     CDS             5083..5664
                     /locus_tag="VKZ48_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76939.1"
                     /translation="MSNQQHQGSYMLHDSVWLISIALMSLLLFVFLFIYARSHTRHED
                     YAPLQTRAYRMRTSVFWILALTLGPTMIYNLFDLPYDVSHGETVQSKPRVIRAVGYQW
                     RWELSSDHAIVGEPLEFHITAADVNHGFGIYDTELRLVAQAQAMPGYTNIVRHTFDTE
                     GVYRILCMEYCGLAHHNMVAEIKVVKRAEARHG"
     gene            5657..7141
                     /locus_tag="VKZ48_03430"
     CDS             5657..7141
                     /locus_tag="VKZ48_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HLU76940.1"
                     /translation="MADYEAPVEHGTRASVLAYIVTSVVTLLLMMLFGLLMRMEQAQY
                     IELGPLWFYQLLTLHGTGMVGVAALAGAGIMWHFLSRYVALSERIFVANLVIFLVGVA
                     LIVGSVLSGKFHGAWTFLYPLPTKSMGMWSVDAAVLFMAGLMVIGIGFVLLHLDIARA
                     VIARYGSLGRALGWPQLFGREEGYGPPPAVVASTMVTIVNLIGLVVGASIITMMTINA
                     YVPSFDIDALLAKNLIYFFGHVFINATIYMAVIAVYELLPRYTRRPWKSNRVFLASWT
                     ASTLMVLFIYPHHMLMDFAMPKWMLVMGHVIGYLNTFPILIVTAYGGLMIVYRSGIRW
                     DMSAKLLFLSLFGWAAGAMPAFIDGTIGVNYIMHNTLWVPGHFHTYLLLGMMTMVFGF
                     MYHIGNTRAAASEGPIDRIALWAFGIATLGFTLSFLYGGKSSVARRYAAHLPEWLPSD
                     RVASVFAALVITAVLVFATRFLLRLPAAARGVPRIAALRGAPAR"
     gene            7138..7743
                     /locus_tag="VKZ48_03435"
     CDS             7138..7743
                     /locus_tag="VKZ48_03435"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HLU76941.1"
                     /translation="MSRALQISTLVSLIALAVYWWGTDGFRAFTAEAARRERVLRDPR
                     PLPSVTLEDQDGRRFGFEDYRGRLVAVEFIYTRCETICRSLGTAFRRIRDHVPAERLG
                     EDFALLTVSFDPRDDRTALQRYARTHGADGTHWRIARVVNAAAIRALLDAFGVIALPD
                     GLGGYEHNAAIHLLDRDGRLVRIGDIEEEPRDFIVIAGLAP"
     gene            7770..8363
                     /locus_tag="VKZ48_03440"
     CDS             7770..8363
                     /locus_tag="VKZ48_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76942.1"
                     /translation="MAAFGLLCALGLPPARSWLEATMTTHMLIQMPLLAAAGYVLGRA
                     FTPSQRAALRRLVGGPTPYVLLASFASSYWMLPRALDAALVDPLAALAKFSSLPLLVG
                     MPLALAWERLGIVGRGFIWTNFFSMLAVLGWLYVAAPVRVCNSYLVADQYEAGWWMIR
                     LAIALFLAWLAGLLVGRAPSARPVRYRPASIPARTAP"
     gene            complement(8338..10374)
                     /locus_tag="VKZ48_03445"
     CDS             complement(8338..10374)
                     /locus_tag="VKZ48_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005375 - copper ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent copper receptor"
                     /protein_id="HLU76943.1"
                     /translation="MSRSFEPKALTLALSVALLYPHFVRAHEHHDEPGPIVVTAPHMR
                     APLEVSFDPRAPQQPLPANDGAGLLKTIPGMNVIRKGGTDGDPVFRGMAASRLNILLD
                     GETILGGCGMRMDPPTAYIFPDSYDKLTLLKGPQTVIHGPGGSAGTVLFERKPTPFAA
                     PGWSIKGAATGASFDRHDEFLDVKGGSPLGYVQGILTHARSGNYRDGNGDTVHSKYER
                     WSATGVLGWTPDRDTRVELSAIRSDGEAAYADRGMDGSSFARSNYGLKFEKRKVSRAI
                     EKIEAQAYYNYIDHVMDNFNVRKPPMNPAMRMLSNPDRETAGGRVAFTLRPGESAKLV
                     LGADLQRNEHTARSGVDYRSKPRVADATFTTYGMFGEWTQYVGESDRLIAGLRSDRWK
                     AEDKRASIASGMMSLGPNPTAGAHREETHTGGFVRYERDLSDDATVYAGFGRAERFPD
                     YWELINKESMTGISGFGTLDAERTKQFDAGLTWRAERMEVFVSAFYSRIDDYILIQSQ
                     VAKGMRRTTVARNIDATTWGGEAGLRYAFDAQWKLSASIAYTRGVNETDDHALGQMPP
                     LEGRIGLDWDGGDWFAGGLLRLVAKQDRYALDEGNIVGQDLGATGGFGVFSLNGGYRW
                     RKDARITVGVDNLFDKTYAESISRGGATIPGFDQTARVNEPGRTYWIKAQFALE"
     gene            complement(10593..10997)
                     /locus_tag="VKZ48_03450"
     CDS             complement(10593..10997)
                     /locus_tag="VKZ48_03450"
                     /inference="COORDINATES: protein motif:HMM:NF022610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 domain-containing protein"
                     /protein_id="HLU76944.1"
                     /translation="MPAASHSQFLFTRRRLRLAAVWGLLALCMHLAGMSAGAFHATAR
                     LAAQSASDHCTVASSATDDAGQTHHGSDPSQHCPLCVFQAAPVPVLIAVLSFLVPAEG
                     DARDYPVADKTAPPVAPQTRHAPPRAPPVLSA"
     gene            complement(11096..12439)
                     /gene="gdhA"
                     /locus_tag="VKZ48_03455"
     CDS             complement(11096..12439)
                     /gene="gdhA"
                     /locus_tag="VKZ48_03455"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HLU76945.1"
                     /translation="MKYKSAGEFLDFVAARNPGQPEFLQAVREVVESVWSYIEQNPRY
                     AEYGLLDRLVEPERAIQFRVAWVDDQGSVRVNRGYRIQHNSAIGPYKGGIRFHPSVNM
                     SILKFLAFEQTFKNSLTTLPMGGGKGGADFDPKGKSPGEVMRFCQAFINELFRYIGAH
                     TDVPAGDIGVGKREVGYMAGMMKKLSNRSDCVFTGKGLSFGGSLIRPEATGYGTVYFA
                     QEMLKHAGRSLEGLRVSVSGSGNVAQYAVEKAMGLGAKVVTVSDSSGTIVDEAGFTPE
                     KLAVLMEVKNQLYGRVSQYAERVGAKFIPGATPWHVPVDVALPCATQNELHAEDAKTL
                     VKNGVKCVAEGANMPSTAEAVDVFIENGVLYAPGKASNAGGVATSGLEMTQNAMRLSW
                     TREEVDARLLEIMQGIHRACVEYGTGKDGKVNYVNGANIAGFVKVADAMLAQGVI"
     gene            complement(12598..13158)
                     /gene="sufT"
                     /locus_tag="VKZ48_03460"
     CDS             complement(12598..13158)
                     /gene="sufT"
                     /locus_tag="VKZ48_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HLU76946.1"
                     /translation="MSRYDEVREVTLTRDCPAVMVPWGTPATLEEGMHAQITQRLGGT
                     FTVVIDGNMYRIEGKHADALGFEVEEEPERDSIAPLSAEQVEEAAWDTLATCYDPEIP
                     IDIVNLGLVYGCEVKETGEGRYHISVEMTLTAPGCGMGTMIADEARDKLLTIPGVDTV
                     DVELVWDPPWDRHMMSEAARLEMGMF"
     gene            complement(13239..13694)
                     /locus_tag="VKZ48_03465"
     CDS             complement(13239..13694)
                     /locus_tag="VKZ48_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HLU76947.1"
                     /translation="MSDMQDELRQLYQEVIFDHNRSPRNFHAMEDANHVAQGHNPLCG
                     DQLTVYVRTEGGVVRDVSFVGQGCAISKASASLMTEAVIGKSEAEVDEMFRGFHAMLT
                     EEEDTEHDLGKLEVLAGVKEFPVRVKCATLAWHTLHNALIGSGEKAKTE"
     gene            complement(13691..>14237)
                     /locus_tag="VKZ48_03470"
     CDS             complement(13691..>14237)
                     /locus_tag="VKZ48_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HLU76948.1"
                     /translation="TGIGALYARWDILRDMPPWQGGGDMIRTVSFEGSTWADAPQRFE
                     AGTPNIAGVVGMAAAIDYVTEIGMDRIAAHEQALLAYATEEVQRLPGVRVIGTAAHKA
                     SILSFLFGDIHPHDLGTILDVEGVAIRAGHHCAMPVMTHYGIAGTARASFALYNDERD
                     VEALLAGLRKAQALFQGDAQR"
BASE COUNT         2465 a         4711 c         4510 g         2551 t
ORIGIN      
        1 ctgtccgccg aagctaaccg gcccctcgaa caccgcgttg cccgcgccgt cgcgcaggaa
       61 actctccggg cgggaatagc ccatgtcgta gtccgcccag ctcgcgccta cggtcagatc
      121 ggtcgcggcc gagggggagt aatgcagatt caactgaccg atccgctgcc gccagggacg
      181 ctcgcccttg tgaccgatcc aataggccgg cttgccgtcg agcgtgctgc tcggccgcgc
      241 gccggtcacg ggcgtacccg gcgggacggg cggaaagggt gccggcggcg tgagcagtac
      301 cggctccgca tccggatcgc cgtcgcttgc gcgataaccc atcaagaagg aaatccccac
      361 gccgccttcg tagcgcttgc gatggatgaa ggtcgcgccg cgttgccgca tgctgcccgc
      421 gcccacgcgc acttccgtca gcggttgatc cggaccggca gtgatgaagt tcaccacgcc
      481 gcccatggcg gcgccgccat agagcgccga ataagggccg cgcacgactt cgacgcgttc
      541 gagattgtcg accggaatgc cgaccacgtt gatgccgccg gtcagcgcgt tgttcacggg
      601 ctggccgtcg accatcacca gcgtgcgggg cgtgcgcgga atgccgcgca atgaaagaac
      661 ggcctgcccc gtcccgggga agttgctccc catcgccgcg ccgcgcacat aaaggccggg
      721 cacatcggcc accgcatcgc ccagacggac gggcttcaac tgttcgagtt ccttgcggct
      781 caccaccgac gtgctcgccg gcacgccttg tatggccgcc ggcgcgcggg tggcggtgac
      841 gacgacggtt tcggtttcga cctgaacggc tccgtcgtcc tgcgcgtgga cagccggaac
      901 gaagaaaaac gcagcgcaga caagccagcg cgctgtgacg cgaatcacaa cttcaaggtc
      961 tgcaggaggt ggttcggaac gggtggtgag cacggctccg actttatgat ggtgcggcgc
     1021 acaaagattg accaggatca agattcttgc tggcctcccg atcaagatcg cgctcaagga
     1081 gtgaacggtt ttaccaaagc gaggaagcaa gtcatgaagc gcaacataag cagtaaagct
     1141 gcagccgtcg tcgtggcatc cctgatgctc ggtgtgacgg gaaatgccac ggcggaggag
     1201 cggccgcacc gcgatctgcc accggcctcg atgcagtatc agggcggcgg ttcgcccatg
     1261 gcaggcgagg agatgcacca caacatcaac ccgaaggcgc cgccgatgac caaggcggag
     1321 ttcgacaagg gtcgccagat gtatttcgag cgttgcgcgg gctgtcacgg cgtgctgcgc
     1381 aagggcgcga cgggcaaacc gctcacgccc gacatcacgc tcgagcgcgg catggcgacg
     1441 ctcaagacct tcatcaagta cggctccccc gcgggcatgc ccaactgggg cacctcaggc
     1501 gaaatgaccg acgaggaaat cgacctgatg gcgcgctata tccagcagga gccgccgacc
     1561 ccgcccgagt tcggcatggc ggagatgaag aagacctgga aggtgctcgt caaacccgaa
     1621 gaccggccga agaagaagct caacaactgg gacattgaca acctgttctc ggtgaccctg
     1681 cgcgataccg gcgagatcgc actgatcgac ggcgcttcga agaagatcat caccttcatc
     1741 aagaccggct acgcggtgca catctcgcgc atgtcggcct cgggtcgtta cctgtacgtg
     1801 atcgggcgtg acggcaagat caacctcatc gacctgtgga tggagacgcc ggacaacgtc
     1861 gccgaaatcc gtatcggcct ggaagcgcgc tcggtcgaaa cctcgaagta caagaagtac
     1921 gaggacgtgt tcgccgtggc cggcgcctac tggccgccgc aatacgtgat catgaaaggc
     1981 gacacgctcg agccgctgaa gatccagagc acgcgcggca cgaccgtgga cacgcaggag
     2041 tatcatcccg agccgcgcgt ggccgcgatc gtcgccatgc acgacaagcc ggagttcatc
     2101 gtcaacgtca aggagacagg caaggcgctg gtcgtcgatt acagcgatct gaacaacctg
     2161 aaaacgacgg aaatcggcgc ggcgcccttc ctgcacgacg gcggctggga tgccagcaag
     2221 cgctacttcc tggtcgcggc caaccagtcg aacaaggtcg cggtcatcga cagtcatgac
     2281 aacgagcttg aagctttggt cgacgtgggc acgattccgc acccggggcg cggcgcgaac
     2341 ttcaagcatc cgaagttcgg tcccgtctgg gcgaccggcc acctcggcga cgaaaccatc
     2401 gcgctgatcg gcaccgatcc gaagaagcat ccgaagagcg cgtggaaggt ggtgcagtcg
     2461 ctgaacgcgc agggcggcgg caacctgttc atcaagacgc atccgaagtc gtcgaacctg
     2521 tgggtcgaca ccccgctcaa cccagatccg aagatcgcgc aaaccgtcgc cgtcttcgat
     2581 atcaacaacc tcgacaagcc ttacgaggtg ctgccgatcg gcgaatgggc gaacgtgggc
     2641 gaaggcgcca agcgcatcac ccagccggag tacaacaagg ccggcgacga agtgtggttc
     2701 tcggtgtgga gcgcgaagaa ccaggagtcg gcgatcgtgg tggtggatga caagacgcgc
     2761 aagctgaaga ccgtgatcaa ggatccgcgc ctcatcaccc cgaccggcaa gttcaacgtg
     2821 tacaacacgc agcacgacgt ctactgacag gagccgacat gaagagaacg acggtgcttc
     2881 acctcatcat cgccgcggcg gcgttcggca tgaccgcgcc cgccttcgcc gacgcggcgc
     2941 tcgcggcgaa gtacagttgc accgcctgcc attcgccgga caagaagatg ctcggccccg
     3001 cgtacaagga cgtggcggcc aagtatcaga atgacgccga ggcgcccgcc cggctggccg
     3061 ggaaggtgaa gcaaggcagc agcggcgtgt gggggccggt ccccatgccg ccgaacgcag
     3121 gcgtgccgga tgacgatgta aagaagctgg tagcctgggt gctcgccggc gcgaagtagg
     3181 accggttccg gtcaactggg gcggctcgat gagccgccct tttttatgcg cgtcggatac
     3241 gagaacgcgg cgacgtgaaa ggccaattca aaatcctgaa acacagagcg cacagagacg
     3301 caccgagaac gcagagaaaa acttcggagc ttcctaaggt ctccgtagaa ccgttcgtgg
     3361 tgagggccca ttgaacggcg ccatggctag atcgatacag cgttcaccct tcgactgatg
     3421 cgctgacgcg catcgctcag ggcgaacgga aaactgccgg cgaggggcct tcgacaggct
     3481 caggacaggc tgagcagtcg aaccatgaac ggtcaactct cgactctccg ctctcggctc
     3541 tcggccgcct tcaacccttc tccgcgctga gccgcttcca tagcgtggtg cgcgacatgc
     3601 cgaggcggcg tgccgcttcg gcgcggttgc cgccgcattc ctgcagcacg cgccggatga
     3661 tgccgcgctc ctcatcgggc gacactacgg ggcggtaata gcgcgtcagc ggcggcgcgt
     3721 aacggtcatg cgtcggcgcg gccggcgctg cgggctgcag aatctcgggc ggcagatgcc
     3781 gcggcaggat gagcgggccg tccgcctgca cggcggcgaa ctcgagtgcg ttgtagagct
     3841 gccgcacatt gcccggccag tcataggcct cgagcgcgtc catggttccc cgcgcgaaac
     3901 gcatcggctc gcgccgatag cgctccgcca gctcgccggc gatcttcccc gcgatgagcg
     3961 cgatgtcgtc tcgccgctcg cgcagcggcg gcacctccag aggcagcacg cgcagccggt
     4021 aataaaggtc ctcgcgaaac tcgccgcgcc gcgccatcgc cgccagatcg cggttggtcg
     4081 cagcaatcac ccgcacatcc accttgcgcg catggttttc gcccagacgc accacttcct
     4141 tctcctgcaa gacgcgcaac agcttggtct gcatggcagg cgaaatttcg ccgatctcgt
     4201 ccagcagcag cgtgccgccg tgcgccgctt cgaagcggcc gatgcgcgtg gtggccgcac
     4261 cggaaaacgc tcccttggcg tgaccgaaca attcagattc gagcagcgtc tcgggcaagg
     4321 cgccgcagtg cagcggaata taggggccgc cgctgcgcgg cgaattgtcg tgcaaggcac
     4381 gggcgacgag ttccttgccg acgccgctct cgccggtgat gagcgcgctc gcatcgctgg
     4441 ccgcggcacg cagaatctgc gcgtagagcg actgcatgac cggcgtgcgg ccgaccagac
     4501 cgcccagccg ccagcgctcg cgcacctcgt tctccagcgc cacttcgcgc gagcggtcgc
     4561 gcagcacaat ggcgcagtac agccccgcat cgttgggcgg gagcagcatg gcccacatgc
     4621 gcagtgccac ttcgctgccg tcgctgcgct cgagcgtgac gtcctgactc ttgcgcgcct
     4681 cgcccggcga aggcgtgagt gcgcaaccac cgcgcacttc gcaagtgcgc gcgcattgcg
     4741 tgccgacgaa caaggtcggg cagagacgct cgatcaactg tccgctgtcg cggccgagca
     4801 cctgcatcgc cgcctcgttg atctccacca cgcgacgatg cgtatcgaga aacagcacgc
     4861 cttcttccaa cgtgtccaga aacaccttga gcgctgcaat cattcccatc gcatccgatc
     4921 cccggcatcg aagtgttcag catgaacgca cgcactgaac gttcaccttg aaccgtaacc
     4981 gcattccagc atgcggcgac aagcggaaaa atgatgccga tcaaacggct gcgttgactg
     5041 gcatgcctct ggctcttggg atgtcttgtt ttcctgccat ccatgtccaa tcaacaacac
     5101 caggggagtt acatgctgca cgattcggtc tggctcatct ccattgcgct gatgagtctg
     5161 ctgctcttcg tctttctgtt catctacgca cgatcccata cgcgccacga ggattacgcg
     5221 ccgctgcaga cgcgtgcata ccggatgcgc acctcggtct tctggatact cgcgctgacg
     5281 ctcgggccga cgatgatcta caacctgttc gacctgcctt acgacgtcag ccacggcgaa
     5341 accgttcagt ccaagccgag agtgattcgc gcggtcggct atcaatggcg ctgggagttg
     5401 agcagcgatc acgccatcgt gggcgagccg ttggaatttc acatcaccgc ggccgacgtc
     5461 aatcacggct tcggcatcta tgacaccgaa ctgcgtctgg tggcgcaggc gcaggccatg
     5521 ccgggctaca ccaacatcgt gcgccacacc ttcgacaccg aaggcgttta tcgcatcctg
     5581 tgcatggagt actgcggcct cgctcaccac aacatggtgg cggaaatcaa agtggtgaaa
     5641 cgggcggagg cgcgtcatgg ctgattacga agcccccgtc gaacacggca cgcgcgcgag
     5701 cgtgcttgcc tacatcgtca cgtccgtggt caccctgctg ctgatgatgc tgttcggcct
     5761 gctgatgcgc atggaacaag cgcagtacat cgaactgggg ccgctgtggt tctaccagtt
     5821 gctgacgctg cacggcaccg gcatggtggg cgttgccgcc ctcgccggcg ccggcatcat
     5881 gtggcacttc ctcagccgct acgtggcgct gtcggaacgg atcttcgttg ccaacctggt
     5941 gatctttctc gtcggtgtgg cgttgatcgt cggcagcgtg ctgagcggaa agtttcacgg
     6001 cgcgtggacc ttcctctatc cgctgccgac caaatccatg ggcatgtgga gcgtggacgc
     6061 ggcggtgctg ttcatggccg gcctgatggt gatcggcatc ggcttcgtgc tgctgcacct
     6121 ggacatcgcc cgcgccgtga tcgcccgtta cggcagcctg ggccgcgcgc tgggctggcc
     6181 gcaattgttc ggccgcgagg aaggctacgg gccgccgccc gcagtcgtcg cctccaccat
     6241 ggtgacgatc gtcaacctca tcggcctggt ggtgggcgca agcatcatca ccatgatgac
     6301 gatcaacgcc tatgtgccct ccttcgacat cgatgcgctg ctggcgaaga acctgatcta
     6361 ctttttcggc catgtgttca tcaacgccac catctacatg gcggtgatcg cagtctatga
     6421 actgctgccg cgctacaccc ggcggccgtg gaaatcgaac cgcgtattcc tcgcctcgtg
     6481 gacggcatcc acgctgatgg tgctgttcat ctacccgcat cacatgctga tggacttcgc
     6541 catgccgaaa tggatgctgg tcatgggcca tgtgatcggc tatctcaaca ccttcccgat
     6601 cctgatcgtc actgcctacg gcggactgat gatcgtgtac cggtccggca tccgctggga
     6661 catgagcgcg aagttgctgt tcctgtcgct gttcggctgg gcggcgggtg cgatgcccgc
     6721 cttcatcgac ggcacgatcg gcgtcaacta catcatgcac aacacgctgt gggtgcccgg
     6781 gcacttccac acctacctgc tgctgggcat gatgaccatg gtgttcggct tcatgtatca
     6841 catcggcaat acccgcgcgg ccgcaagcga gggcccgatc gaccgcatcg cactgtgggc
     6901 tttcggcatc gccacgctcg gcttcacgct gagcttcctc tacggcggca agtcgagcgt
     6961 cgcccgtcgc tacgccgcgc acctgcccga gtggctgcct tccgatcgcg tcgccagcgt
     7021 cttcgcggca ctggtgatca ccgcggtact ggtgttcgcg acgcgcttcc tgctgcgtct
     7081 gcctgcggcg gcgcgcggcg tgcctcggat cgctgccctg cgcggcgcgc ccgcacgatg
     7141 agccgggcgc tgcagatctc gacgctggtg tcgctgattg cgctggcggt gtactggtgg
     7201 ggcacggacg gctttcgtgc cttcaccgcg gaagccgcgc ggcgcgagcg cgtcctgcgc
     7261 gatccgcgtc cgctgccatc cgtcacgctg gaggatcagg acggccgccg cttcggcttc
     7321 gaggattatc gcggacggct ggtcgcggtg gagttcatct atacgcgctg cgagacgatc
     7381 tgccgcagcc tgggcaccgc gtttcggcgc atccgcgacc acgtaccggc cgagcgcctc
     7441 ggcgaggact tcgcgctgct gacggtgagc ttcgatccgc gggacgatcg cacagcgctg
     7501 caacgctatg cgcgcacgca cggtgcggac ggtacgcact ggcgcatcgc gcgcgtggtc
     7561 aacgccgcgg cgatccgcgc attgctcgac gccttcggcg tgatcgccct gcccgacggg
     7621 ctcggcggct atgaacacaa tgccgccatc catctgctcg atcgcgacgg ccggctggtg
     7681 cgcatcggcg acatcgagga ggaaccacgc gacttcatcg tcatcgccgg gcttgcgcca
     7741 tgaacgggcc gcgcgcacca caccctgcca tggcggcatt cggactgctg tgcgcgctcg
     7801 gcctgccgcc tgcgcgcagt tggctcgaag cgacgatgac gacgcacatg ctgatccaga
     7861 tgccgctgct ggcggcggca ggttacgtgc tcggccgcgc cttcaccccc tcgcagcgcg
     7921 ccgcgctgcg tcggctggtc ggcggcccga caccctatgt gctgcttgcg tcgttcgcgt
     7981 cgagctattg gatgctgccg cgcgcgctcg acgcggcgct ggtcgatccg ctcgccgcgc
     8041 tggcgaagtt ctccagtctg ccgctgctcg tcggcatgcc gctcgcactg gcatgggagc
     8101 ggctcggcat cgtcggccgc ggcttcatat ggaccaattt cttcagcatg ctcgcagtgc
     8161 tgggctggct ttatgtcgcc gcgccggtgc gcgtgtgcaa cagctatctg gtcgccgatc
     8221 agtacgaggc cggctggtgg atgatccgtc tggcgatcgc gctattcctg gcgtggctcg
     8281 ccggcctgct ggtcgggcgc gcgccgagcg cgcgccccgt tcggtaccgc ccggcttcta
     8341 ttccagcgcg aactgcgcct tgatccaata agtacggccc ggttcgttca cgcgcgccgt
     8401 ctggtcgaac cccggtatgg tcgcgccgcc ccggctgatg gattcggcat aggtcttgtc
     8461 gaacagattg tccacgccga ccgtgatccg cgcatccttg cgccagcggt agccgccgtt
     8521 gagggagaac acgccgaagc cgccggtggc tcccagatcc tggccgacga tgttgccttc
     8581 gtcgagcgca tagcgatcct gttttgcgac cagccgcagc aatccgcccg cgaaccagtc
     8641 gccgccgtcc cagtccaggc cgatacggcc ttcgagcggc ggcatctgac ccagcgcatg
     8701 gtcgtcggtc tcattgacgc cgcgggtata ggcaatgctc gcgctgagct tccactgtgc
     8761 gtcgaaggcg taccgcagac ccgcctcgcc gccccaggtc gtggcatcga tgttgcgcgc
     8821 gacggtcgtc ctgcgcatgc ccttggcgac ctgcgactgg atgagaatgt aatcgtcgat
     8881 gcggctgtag aaagcggaaa cgaaaacttc catccgctcc gcgcgccagg tcaagcccgc
     8941 atcgaactgc ttcgtgcgct cggcgtcgag cgtcccgaag ccgctgatgc ctgtcatgct
     9001 ttccttgttg atgagttccc agtagtcggg aaagcgttcg gcacgcccaa agccggcgta
     9061 aacggtggcg tcgtccgaca gatcgcgctc gtagcgtacg aagccgccgg tatgcgtttc
     9121 ttcgcgatgc gcgccggcgg tcgggttcgg ccccaaagac atcatgccgc ttgcgatcga
     9181 ggcccgcttg tcctccgcct tccagcggtc gctgcgcaga cccgcgatga ggcggtcgct
     9241 ttcgccgaca tactgcgtcc actcgccgaa catcccgtag gtcgtgaagg tcgcatccgc
     9301 cacgcgcggc ttcgaacggt aatcgacgcc gctgcgggcc gtgtgctcgt tgcgctgcaa
     9361 atccgcgccg agaacgagct tggccgactc accgggacgc agcgtaaacg ccacgcgccc
     9421 gccggccgtc tcgcgatctg ggttgctcag catgcgcatc gccgggttca tcggcggctt
     9481 gcgcacgttg aaattgtcca tcacgtgatc gatgtagttg taataggcct gcgcctcgat
     9541 tttctctatc gcccgcgaaa ccttccgctt ctcgaacttg agaccgtagt tgcttcgcgc
     9601 gaagctgctg ccgtccatgc cgcggtcggc ataggcggct tcgccgtcgc tgcggatggc
     9661 cgacagttcc acgcgcgtat cccggtccgg cgtccagccg aggacgccgg tagcgctcca
     9721 ccgctcgtat ttcgagtgca ccgtgtcgcc gttgccgtcc cgatagttgc ccgagcgcgc
     9781 atgggtgagg atgccctgca catagccgag cggactgccg cccttcacat ccagaaactc
     9841 atcgtgccgg tcgaagcttg cgccggttgc cgcacccttg atcgaccagc cgggtgcggc
     9901 gaagggcgtg ggcttgcgtt cgaacagcac cgtacccgcc gaaccgccgg gcccgtgaat
     9961 cacggtctgc gggcccttga gcaacgtcag cttgtcatag gaatcgggga agatgtatgc
    10021 ggtgggcggg tccatgcgca taccgcaacc gccgaggatg gtttcgccgt cgagcaggat
    10081 attgagccgc gacgcggcca tgccgcgaaa caccgggtcg ccgtccgtgc cgcctttgcg
    10141 gatgacgttc atgccgggaa tggttttgag caggcctgcg ccgtcgttgg cgggcagcgg
    10201 ctgttgcggc gcgcgcggat cgaagctcac ctccagcggc gcgcgcatgt gcggcgccgt
    10261 gacgacgatt ggaccgggtt cgtcgtgatg ttcgtgcgca cgaacgaaat ggggatacag
    10321 caatgcgacg ctcagcgcga gcgtgagcgc cttcggttcg aaggaacggg acatgatttg
    10381 ttctctctac gtatagatcg atgcgcctgc gcgacgcgcg cgcgcgggcg aagccgttcc
    10441 ccccgtggga gaacggagcg cgaaaccgcg gaagctgcgc tgatggggct ctttgacgta
    10501 cgcggccgcc ggcgcatggc ggcgaagcgg caccgtgcac tcgatcgtgc aacggcgcgc
    10561 gaagactcaa tcaggcattt cctgacggct gatcaggcgg agagaacggg cggcgcccgc
    10621 ggcggcgcgt ggcgcgtttg cggcgcgacc ggcggcgccg tcttgtcggc cacagggtaa
    10681 tcgcgggcat cgccttccgc cgggacgagg aaggacagta ccgcgatgag caccggcacc
    10741 ggagcagcct ggaacacaca caagggacag tgctgcgacg gatcgctgcc gtgatgcgtc
    10801 tgtcccgcat catcggtcgc cgatgatgcg acggtgcagt gatccgacgc gctctgcgcg
    10861 gcaagtctcg ccgtcgcgtg aaaggccccg gcactcatgc ccgccaagtg catgcacagc
    10921 gcgagcaatc cccagacagc ggcaagccgc agcctgcgac gcgtgaagag gaactgcgaa
    10981 tgggaggcgg cgggcatggg acgcgactct atcgcatcga cccgccgcgc ctctgacgtg
    11041 gatcaaacaa aaacggcctc ccgcgggagg ccgtttcgtt ggcgttgcgt gcgactcaga
    11101 tcacgccctg ggccagcatc gcatcggcga ccttgacgaa gccggcgatg ttcgcgccgt
    11161 tgacgtagtt caccttgccg tccttgccgg tgccgtactc gacgcaggcg cgatggatgc
    11221 cctgcatgat ctcgagcagg cgggcatcga cttcctcgcg cgtccaggaa aggcgcatgg
    11281 cgttctgcgt catctccaga ccggaggtgg cgacgccgcc ggcgttgctc gccttgcccg
    11341 gcgcgtacag cacgccgttt tcgatgaaca catcgaccgc ttcggcggtg gaaggcatgt
    11401 tcgcgccttc ggccacgcac ttgacgccgt tcttcaccag cgtcttcgca tcttccgcat
    11461 gcagttcgtt ctgcgtcgcg cagggcagcg cgacgtcgac cggcacgtgc cagggtgttg
    11521 cgccgggaat gaacttggcg ccgacgcgtt cggcgtactg gctgacgcgg ccgtaaagct
    11581 ggttcttcac ctccatcagc acggcgagtt tctcgggcgt gaagcccgct tcatcgacga
    11641 tggtgccgct ggagtcggaa acggtaacga ccttggcgcc cagacccatg gctttctcga
    11701 ccgcgtattg cgcgacgttg ccggagccgg acaccgaaac ccgaagccct tcgagcgagc
    11761 ggcccgcgtg cttgagcatt tcctgcgcga aatagaccgt gccgtagccg gtcgcttcgg
    11821 gccggatgag cgagccgccg aagctcaacc ccttgccggt gaacacgcag tcggagcggt
    11881 tggacagttt cttcatcatg ccggccatgt agccgacttc gcgtttgccc accccgatgt
    11941 cgccggccgg gacgtcggta tgcgcgccga tgtagcggaa cagttcgttg atgaaggcct
    12001 ggcagaagcg catcacttcg cccgggctct tgcccttggg atcgaagtcc gctccgccct
    12061 tgccgccgcc catgggcagc gtagtgagag agttcttgaa cgtctgttcg aaggccagga
    12121 acttgaggat cgacatgttc accgaggggt gaaagcggat gccgcccttg tacgggccga
    12181 tggccgagtt gtgctggatc cggtagccac ggttgacccg cacgctgccc tggtcgtcca
    12241 cccaggcgac gcggaactgg attgcccgct ccggctccac cagacgatcg agcagcccat
    12301 actccgcata gcgcggattc tgttcaatgt atgaccatac gctctcgacc acttcgcgga
    12361 ccgcctgcag aaattccggc tggcccgggt tgcgcgcggc gacgaagtcg agaaattcgc
    12421 ccgcgctctt gtacttcaac acgcccgtgt tcctttgcta ggtgagagat gagggggcgt
    12481 aattgtcgcc gaagtggtgc atgtttgcac cgttttcgtg cgaccagccg cccattttgt
    12541 tcaaagtctt ggcgttcagg cgcggttttg atgcgccata cgccccagtc tggtgcgtca
    12601 gaacatgccc atttccaggc gggcggcctc gctcatcatg tgccggtccc acggcgggtc
    12661 ccagaccagt tccacgtcca cggtatccac gccgggaatg gtcagcagct tgtcgcgcgc
    12721 ctcatcggcg atcatcgtgc ccatgccgca accgggcgcg gtcagcgtca tctcgaccga
    12781 gatgtggtag cgcccctcgc ccgtctcctt gacctcgcag ccgtacacca ggccgaggtt
    12841 gacgatgtcg atggggatct ccgggtcgta acaggtggcg agtgtgtccc aggccgcttc
    12901 ttccacctgc tccgccgaaa gcggcgcgat cgagtcgcgt tcgggctctt cctcgacctc
    12961 gaagccgagc gcatcggcat gcttgccctc gatgcggtac atgttgccgt cgatcaccac
    13021 ggtaaaggtg ccgcccaggc gctgcgtgat ctgcgcatgc atgccttctt ccagcgtcgc
    13081 cggcgtgccc caaggcacca tcaccgcggg acagtcgcgc gtcagcgtga cctcccgcac
    13141 ttcgtcatat cggctcattc gcttaccttt gaaacactag gccacgaacg ctcgcgtatg
    13201 ccgctcgtgg ccgcattggt tcttcgctct cgacagcctt actccgtctt cgccttttcg
    13261 cccgaaccga tcagcgcgtt gtgcaaggtg tgccatgcga gcgtcgcgca cttcacgcgt
    13321 accggaaatt cctttacgcc ggccagaacc tccagctttc ccagatcatg ctccgtatct
    13381 tcctcttcgg tcagcatggc gtggaagccg cgaaacatct cgtcgacctc cgcttcggat
    13441 ttgccgatca ccgcttcggt catcagcgag gccgaggcct tggaaatcgc gcagccctgg
    13501 ccgacgaaac tcacgtcccg caccacgccg ccttcggtgc gcacataaac cgtcagttga
    13561 tcgccgcaca gcgggttgtg cccctgcgcg acgtggttcg cgtcttccat ggcgtggaag
    13621 ttacgcggac tgcggttgtg atcaaaaatg acttcctgat aaagctgccg caactcgtcc
    13681 tgcatgtcgc tcatcgctgc gcatccccct ggaacagcgc ctgcgccttg cgcaaacccg
    13741 cgagcagcgc ttcgacatcg cgttcgtcgt tgtacagcgc gaaggaggcg cgcgccgtcc
    13801 cggcgatgcc gtaatgcgtc atgaccggca tggcgcagtg atggcccgcg cggatggcca
    13861 cgccttcgac gtcgaggatg gtgccgagat cgtgcggatg gatgtcgccg aacaggaagg
    13921 acaggatgct cgccttgtgc gccgccgtgc cgatgacgcg cacgcccggc agccgctgca
    13981 cttcctcggt cgcgtacgcc agcagcgcct gctcatgcgc cgcgatgcga tccatgccga
    14041 tctcggtcac atagtcgatg gccgccgcca tacccaccac cccggcgatg ttcggcgtgc
    14101 ccgcctcgaa acgctgaggc gcgtcggccc aggtgctgcc ttcgaaactc acggtgcgga
    14161 tcatgtcgcc gccgccctgc cagggcggca tgtcgcgcag gatgtcccaa cgcgcgtaca
    14221 gcgcgccgat gcccgtc
//
LOCUS       DATGOU010000060         7754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_140328, whole genome shotgun sequence.
ACCESSION   DATGOU010000060 DATGOU010000000
VERSION     DATGOU010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7754
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140328"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2367)
                     /locus_tag="VKZ48_03475"
     CDS             complement(<1..2367)
                     /locus_tag="VKZ48_03475"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLU76949.1"
                     /translation="MALALCCAAAVLPQQAHTEEEVAMADAPNWLVQQVRRFRSHPHL
                     DRGYRLEQAGRLYEARAELGKAVVADPRNAEVRLHYVALLERLGEPEQVLRHTQPLLK
                     DQDYRAAALLARARAFRALDRLEEASVALKEVAAAQDMPAEQRIDALDALVDLELKRG
                     RYGQAHDALALLPHRDDFRLRLRRGIVHDALGRYDDAAYEFAAALHLAEDSEQRVQAL
                     AYLGQNAFKREDWAAAQAHYDAALALDPRNVELMRALTQAAHRAGDAEQALRWAEEVM
                     TVAPTPADREVVANLRYATGDHAGAVHDYEKLLAETDDADKRVHLYAAIGYAHYDMGR
                     YAEAAQAFDAAVRLRGTASLRKALAAAQAAARPPEGEMESLQAALRKHPTAKVHLRLG
                     ALYVERGDAQAAVRHFEAATQGNAAPRHRATAYRRLWPIRYAQGDLDAARGALEALIK
                     LAPKDAAALRALAQIEFEQGDTASAVAHLWRSVQLKPSVSAYRSLAFATASMGDWEGA
                     VRANRGWLAMRKLSASDRAEAYMRLATAYAEAGDTASEIETLQQALAEGFDNRPVRER
                     LGMALFKAQRWREALPHFRALYQRKANALTALSLSRCYSAMEKTGLAVHYGREALSKA
                     EELPPDERAQLQAELAYLYADESEFSAAATMWRQVIARDPRPIHVYHYAVAQHRAGAL
                     GEARAAWESIADGALPPEQEVERLNQLAKFHSTEGDPGGAVALYAKADALMPTAYHQY
                     SIGLAMQQLGRPDDAIRAFEQALAREPQPGYALALGYAYVNAGRHAEAA"
     gene            complement(2425..4089)
                     /locus_tag="VKZ48_03480"
     CDS             complement(2425..4089)
                     /locus_tag="VKZ48_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HLU76950.1"
                     /translation="MNARLYAIVAAFALVLWAPVAPAQTTPIKALVLYDAPSTGDYRK
                     LGFAYAIMLRNLLGHWNTDVDVQPVEQYASGQVDNYDVTFYMGGIYDNQLPPNFIGDV
                     HNTTQTVVWFKYNLWQYAWNPSYDFNNRYGLGFYGLLGMNAQPTEQNPNPGFFDTVDY
                     KGKSLVKYYSFQNGVVAADPDIGIVGVENPNKAEAVVSIRNPQTGQTAPYIVRAGNFW
                     YVADMPFSYIGPRDRYLVICDVLHDMLGVQTPASKRAMVRLEDVGALVLPSTMVTLIN
                     YMHGRGIPFSVAAIPFYRDPLGQYNDGVPMEIHLNDAPNLKASLDYALARGGRIVMHG
                     YTHQYESKRNIHTAVSGDDFEFWDAVENTPVIEDSIAWALGRLDAGLAELTLNGYQAF
                     AWEAPHYQSSPKAIRAAAQRFPTTYQRVVYYTSDEPDFNPSNPARDFAVGQFFPYVIE
                     RDYYGQRVLPENLGNIEYDIREIDPTSNFDYTWQDLLLNAEMGQVVRDGFASFFFHPF
                     WLDPDIGTPGFADFKSLVEGITALGYTWVGAADIVPPGWLGLSMGP"
     gene            complement(4138..4551)
                     /locus_tag="VKZ48_03485"
     CDS             complement(4138..4551)
                     /locus_tag="VKZ48_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76951.1"
                     /translation="MRSARDERDNDHWRLEMNTGMKLLLTAALAVSLLVPVAALAQGK
                     QPPAKEARGQAKAAKVMKVDTKKPKPVRIKAPPKPGVKTVVAPGTWRVHRHRGNSPLF
                     EPGREKEWVEHPGPGKRPVIKRQGPPGKGMAKGHR"
     gene            complement(4532..6139)
                     /locus_tag="VKZ48_03490"
     CDS             complement(4532..6139)
                     /locus_tag="VKZ48_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012033.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HLU76952.1"
                     /translation="MEASDVLAVAMVGAMVLLIFVSVAFLLSGLDDLFIDICYFCRAA
                     YRRLFVERLRVPVTESALLARPQQPIAVMVPAWDESAVIRSMLLNMVRSLNYDAYHVF
                     VGTYPNDEATHREVDAVCALHPNVHRVVTPHDGPTNKADCLNWILQGVKRVEREQHIH
                     FEIFVMQDCEDVIHPLCYRVFNLLIPRHDMVQVPVLSLLPKWHQLTAGHYIDEFCQLH
                     YKDLVVRELLNRSLPAAGVGCAFSRRAFDTIAEQNNNIVFSVDSLTEDYEFGFRMKEY
                     GLKQIFAQVWVQEQRELAAFPSPNLVCVREFFPSNFRAAVRQKSRWVLGITLQGWENL
                     GWLRGIATRYMLVRDRKGLLTNLVNVLAYLVVFAVAGMWLVNHFQPDSYRYPALLEKG
                     TWLWYVILANSVLLCHRAAMRAYCVYRIYGAQQALLSLPRMVWGNFINFLATARAIKQ
                     YARYLRTGKLIAWDKTQHVYPSEEQLGGTAQPRLGEVLLQRQLITQDQLNDALARQRE
                     RPSALGQILKEMGAIQEAELSHALGAR"
     gene            complement(6148..7371)
                     /gene="wecB"
                     /locus_tag="VKZ48_03495"
     CDS             complement(6148..7371)
                     /gene="wecB"
                     /locus_tag="VKZ48_03495"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HLU76953.1"
                     /translation="MGTRPEIIKMAPVYRALRASRLGVTILHTGQHEEMAWPLYEFFG
                     MRPDHIVNLSRSSAALSHLSARLLEEIASVVEVVRPKAVLVHGDTSSTLNAALAAFYQ
                     QIPIGHVEAGLRSGAMYDPFPEEMNRSLVGRVAQWHFAPTVQAAENLRSEGVPQERIH
                     IVGNTIVDAALWGGAHLGARSDFATELIDCGLHQVPQLANGRRLLLVTAHRRENWGSG
                     IAGIACAVRRALEAHEELCAVWPVHANRTVRDTVYAAFQGMARDPASRLVLCEPLNYA
                     ALLWVLQRAWLALTDSGGIQEEAIAVKVPVLVLRETTERPELLDAGGGILTGTDPDSI
                     CAQLDALLGDEVRHTRMRQARNPFGDGHAAEYIARILEQALVPPERMEVAAGARVSLF
                     PDWEVAALRSEAHAG"
BASE COUNT         1396 a         2578 c         2382 g         1398 t
ORIGIN      
        1 cgccgcctcg gcatggcggc cggcgtttac gtaagcgtag ccgagcgcca gcgcataacc
       61 cggctggggt tcgcgcgcca gcgcctgctc gaacgcccgt atcgcgtcat ccgggcgccc
      121 aagctgctgc atcgccaagc cgatgctgta ctggtgatag gcagtcggca tgagcgcgtc
      181 cgccttggcg tacagcgcca ccgcacctcc cggatcgccc tcagtgctat ggaacttggc
      241 gagctgattc agccgctcga cttcctgctc gggcggcagt gcgccatcgg cgatcgactc
      301 ccatgccgcg cgcgcctctc ccaacgcgcc cgcgcgatgc tgcgcgacgg cgtagtgata
      361 cacatggatc ggccgcggat cgcgtgcgat gacctgccgc cacatggtcg ccgcagcaga
      421 gaactccgat tcgtcggcat agagataggc cagttccgcc tgcagttgcg cgcgttcgtc
      481 gggcggcaat tcttcggcct tgctcaacgc ttcgcgaccg taatgcacgg ccagcccggt
      541 tttttccatc gcgctgtagc agcgcgacaa cgacagcgcc gttagggcat tcgccttacg
      601 ctgatagagc gcacgaaaat gcggcagcgc ttcgcgccaa cgctgtgctt tgaaaagcgc
      661 catacccagt cgctcgcgca ccggacgatt gtcgaatcct tctgcgagcg cctgctgcag
      721 cgtttcgatc tcagatgcgg tatcgcctgc ttcggcatag gccgttgcca gtcgcatata
      781 ggcttccgct cgatcggacg ccgacagctt ccgcattgcc agccacccgc gattggcgcg
      841 caccgcacct tcccaatcgc ccatcgaagc cgtcgcgaat gcaagcgaac gatacgccga
      901 cacgctcggc ttgagctgta cgctgcgcca cagatgggcg acggccgagg ccgtgtcgcc
      961 ctgttcgaac tcgatttgcg ccagcgcccg caacgccgcc gcatctttgg gcgccagttt
     1021 gatcagcgct tccagcgcgc cacgcgctgc atcgagatcg ccctgcgcgt agcggatcgg
     1081 ccacagccgt cgataggcgg tggcgcgatg gcgcggcgcc gcgttgcctt gcgtcgccgc
     1141 ttcgaagtgc cggacggccg cttgcgcgtc gccccgttcg acatacagtg cgccgaggcg
     1201 taaatgcact ttcgcagtcg ggtgcttccg caacgccgcc tgcagcgatt ccatttcccc
     1261 ttccggcgga cgtgcggccg cctgcgcggc ggccagtgcc ttgcgcaagc tcgccgtgcc
     1321 gcgcagccga acggccgcgt cgaaagcctg cgccgcctcc gcgtagcgcc ccatatcgta
     1381 atgggcataa ccgatcgcgg cataaagatg cacacgcttg tccgcgtcgt ccgtttcggc
     1441 cagcagcttc tcgtagtcgt gcactgcacc cgcatgatcg ccggtcgcgt agcgcagatt
     1501 ggccaccact tcgcgatcgg ccggcgtcgg tgcgaccgtc atcacctcct ctgcccagcg
     1561 gagcgcctgc tccgcgtcgc cggcacgatg cgcagcctgt gtcaacgcgc gcatcagttc
     1621 gacattgcgg ggatcgagcg ccagcgccgc atcgtaatgg gcctgtgccg ccgcccagtc
     1681 ctcgcgcttg aacgcgttct gccccagata ggccagcgcc tgcacgcgtt gctcggaatc
     1741 ctccgcaagg tgcaaggcgg cggcgaactc atacgccgcg tcgtcatagc gccccagggc
     1801 atcgtgcacg atgccccgcc gcaaacgcag acggaaatcg tcgcgatgcg gcagcaatgc
     1861 aagtgcgtcg tgcgcttggc cgtagcgccc tcgcttcagt tccagatcga ccagcgcatc
     1921 gagcgcatcg atacgctgct cggccggcat gtcttgcgcg gccgccacct ccttgagcgc
     1981 gacactcgct tcttccaacc gatcgagcgc gcgaaaggcc cgcgcccgtg caagcaacgc
     2041 cgcggcgcgg taatcctgat ccttcagcag cggctgcgtg tggcgcagca cttgctccgg
     2101 ttcgccgagc cgttccagca acgcgacata atgaaggcgc acctccgcat tgcgcggatc
     2161 ggcgacaacc gctttgccca attcggcgcg cgcctcatac agtcggccgg cctgttcgag
     2221 gcgataaccg cgatccagat gcggatggct gcggaaacgg cggacctgct gcaccagcca
     2281 gttcggcgca tccgccattg ccacctcctc ctcggtgtgc gcctgttgcg gcagcactgc
     2341 ggcagcgcag cacagcgcaa gcgccatacc gaagcacgct cgtttccgct tccccctgcg
     2401 gaacatggcc gttgcacccg gcccttacgg ccccatgctc aaacccagcc aaccgggcgg
     2461 cacgatgtcc gccgcgccga cccaggtgta acccagcgcg gtgatgcctt ccaccagact
     2521 cttgaaatcg gcgaaacccg gcgtgccgat gtccgggtcg agccagaacg ggtggaagaa
     2581 gaaggaggcg aagccatccc gcaccacctg ccccatctcg gcgttcagca gtaggtcctg
     2641 ccaggtgtaa tcgaagttgg acgtgggatc gatctccctg atgtcgtact cgatgttgcc
     2701 cagattctca ggcagtacac gctggccgta gtaatcgcgc tcgatcacgt agggaaagaa
     2761 ctgtcccacg gcaaagtcgc gcgcgggatt ggacggattg aagtccggct catccgaggt
     2821 gtagtacacg acccgctgat aggtggtcgg aaagcgttgc gcggccgcgc ggatcgcctt
     2881 gggcgaggac tggtagtgcg gcgcctccca ggcgaaagcc tgataaccgt tcagcgtcag
     2941 ctccgccagg ccggcgtcga gacggccgag cgcccaggcg atcgaatctt caatgacggg
     3001 cgtgttctcc accgcatccc agaactcgaa gtcgtcacca ctgaccgcgg tgtgaatgtt
     3061 gcgcttggat tcgtactgat gcgtatagcc gtgcatcacg atgcgcccgc cgcgcgccaa
     3121 tgcgtaatcg agcgaggcct tgaggttcgg tgcgtcgttc agatggatct ccatcggcac
     3181 gccgtcgttg tactgaccga gcggatcgcg atagaaggga atggccgcca ccgagaacgg
     3241 gatgccccgc ccgtgcatgt aattgatcag cgtgaccatg gtcgagggca gcaccagcgc
     3301 acccacatcc tcgagtcgca ccattgcgcg cttgcttgcc ggcgtctgca cgccgagcat
     3361 gtcgtgcagg acgtcgcaga tcaccagata gcggtcgcgc ggaccgatgt aggagaacgg
     3421 catgtcggcg acgtaccaga agttgccggc gcgcacgatg taaggcgccg tctgaccggt
     3481 ttgcggattg cggatcgaga ccaccgcctc agccttgttg ggattctcca cgccaacgat
     3541 gccgatgtcc gggtcggccg cgaccacgcc gttctgaaag ctgtagtact tgaccagcga
     3601 tttgcccttg taatccacgg tatcgaagaa accgggattc gggttctgct cggtcggctg
     3661 cgcattcatc ccgagcagcc cgtagaaacc gaggccgtaa cgattgttga agtcgtagct
     3721 gggattccag gcgtattgcc agaggttgta cttgaaccag accaccgtct gcgtcgtgtt
     3781 gtgcacgtca ccgatgaagt taggcggcaa ctggttgtcg tagattcccc ccatgtagaa
     3841 ggtgacgtcg tagttgtcca cctgcccgct ggcgtactgc tcgacgggct gcacatccac
     3901 atcggtgttc cagtgcccga gcagattgcg cagcatgatc gcgtacgcaa aaccgagctt
     3961 gcggtagtcg ccggtcgacg gcgcgtcgta gagcacgagc gctttgatgg gcgtggtctg
     4021 tgcaggcgcg acgggcgccc acagcaccag ggcgaatgcc gcgacaatgg cgtacaagcg
     4081 tgcgttcatg attctctcct tggcgcgcgc agatgacacc tgcgcagggc gacgcggcta
     4141 tcgatggccc ttggccatcc ccttgccggg ggggccttgg cgtttgatga cgggccgttt
     4201 gcccgggccc ggatgctcta cccattcctt ttcgcgaccc ggctcgaaca acggcgagtt
     4261 gccgcgatgg cggtgtaccc gccaagtgcc cggcgcgacg accgtcttca cgcccggctt
     4321 gggcggcgcc ttgatgcgaa ccggcttggg ttttttcgtg tccaccttca tcaccttggc
     4381 ggcctttgcc tgccctcgtg cctctttggc cggcggctgc ttgccctgcg ccagcgcggc
     4441 aacagggacc agcagcgaca cggcaagcgc cgccgtcaac aacagtttca ttccggtgtt
     4501 catctccaac ctccagtggt cgttatcgcg ctcatcgcgc gccgagcgca tggctgagct
     4561 cggcttcctg aatggcgccc atctccttga gaatctggcc gagcgccgac ggccgctcgc
     4621 gctgccgcgc gagcgcatcg ttcaactgat cctgggtaat gagctgccgc tgcagcagca
     4681 cctcgccaag gcgcggctgc gcggtgcctc cgagttgctc ttccgacgga tagacatgct
     4741 gggtcttgtc ccacgcgatc agcttgccgg tacgcagata gcgcgcgtac tgtttgatcg
     4801 cgcgcgcggt cgccaggaaa ttgatgaagt tgccccacac catgcgcggc aaggaaagca
     4861 gcgcctgctg cgcgccgtag atgcggtaca cgcaataggc tcgcatcgct gcgcgatggc
     4921 acagcagtac gctgttggcg agaatcacgt accagagcca ggtgcccttc tccagcaacg
     4981 cggggtagcg ataggaatcg ggctggaaat gattcaccag ccacatgccc gccacggcga
     5041 agacgaccaa ataggcgagt acgttgacca gattggtcag cagccccttg cgatctcgca
     5101 ccagcatgta tcgcgtcgcg atgccgcgca gccagccgag gttctcccag ccctgcagcg
     5161 tgatgccgag cacccaacgc gacttctgcc tcacggcagc gcggaaattc gagggaaaga
     5221 attcgcgcac gcagaccagg ttcggcgagg gaaaggctgc cagctcgcgc tgctcctgca
     5281 cccacacctg cgcgaagatc tgcttcaggc catactcctt catccggaaa ccgaactcgt
     5341 agtcttcggt cagcgagtcg accgagaaca cgatgttgtt gttctgctcg gcgatggtgt
     5401 cgaaggcgcg acgactgaag gcgcagccca cgcctgccgc cggcaggctg cggttgagca
     5461 gttcgcgcac gaccagatcc ttgtagtgca actggcaaaa ctcgtcgatg tagtgccctg
     5521 cggtgagttg atgccatttc ggcaacagcg acagcaccgg cacctgcacc atgtcgtgcc
     5581 gcggaatgag gagattgaag acgcggtagc acagcgggtg gatgacgtct tcgcagtcct
     5641 gcatgacgaa gatttcgaaa tgaatgtgct gctcgcgctc gacgcgcttg acgccctgca
     5701 gaatccagtt gagacagtcg gccttgttgg tcggcccgtc gtgcggcgtg acgacccgat
     5761 ggacgttcgg atggagcgca cacaccgcat ccacctcgcg gtgtgttgcc tcatcgttgg
     5821 gataggtgcc gacaaagacg tggtacgcgt cgtaattgag cgagcgcacc atgttgagca
     5881 gcatggaacg gatcaccgcg gactcgtccc atgccgggac catcaccgct atcggttgct
     5941 gcgggcgtgc cagcagcgcg ctctccgtca ccggcacacg caggcgctca acgaacaggc
     6001 gccgataggc cgcgcggcag aagtagcaga tgtcgatgaa cagatcgtcc agaccgctca
     6061 gcaggaaggc gacactgacg aagatcagca gcaccattgc gccgaccatc gcgaccgcga
     6121 gtacgtcgct cgcttccatg gttcggccta acccgcatgc gcctcagacc gcagcgctgc
     6181 gacttcccag tccgggaaca gggaaacgcg cgcgcctgcg gctacttcca tgcgctccgg
     6241 cggcaccaga gcctgctcca ggattcgcgc gatgtactcg gcggcatgcc cgtcgccaaa
     6301 gggattgcgc gcctggcgca tgcgcgtgtg gcgcacttca tcgcccagca aagcgtcgag
     6361 ttgcgcacag atcgaatccg gatcggtgcc ggtcagaatg ccgccgccgg cgtccagcag
     6421 ttccggccgc tcggtcgtct cgcgcagaac gagcaccggc acttttaccg cgatggcctc
     6481 ctcctgaatc ccccccgagt cggtaagcgc gagccaggcg cgctgcagca cccataacag
     6541 cgcagcatag ttgagcggct cgcacagcac caggcgcgat gccggatcgc gggccatgcc
     6601 ttggaacgcc gcgtacaccg tatctcgcac tgtgcgattg gcatgcaccg gccagacggc
     6661 gcagagttct tcatgcgctt cgagcgcacg tcgcaccgcg cacgcgatcc cggcaatacc
     6721 gctgccccaa ttttcgcgcc gatgcgccgt cacgagcagc aaccgacgcc cgttcgccag
     6781 ttgcggcacc tggtggagac cgcagtcgat caattcggtg gcgaaatccg aacgcgctcc
     6841 caggtgcgcg ccgccccaca gtgcggcgtc gacgatggta ttgccgacga tgtggatgcg
     6901 ttcctgcggc acgccttcgc tgcgcagatt ttcggcggcc tgcacggtcg gtgcgaaatg
     6961 ccactgcgcg acccgcccga ccagcgagcg attcatctct tccggaaacg gatcgtacat
     7021 ggcaccgctg cgcaacccgg cttccacatg gccgatgggg atctgctggt aaaacgcggc
     7081 cagcgccgcg ttaagcgtgc tcgacgtgtc gccgtgcacc agcacggcct tcggtcgcac
     7141 gacctcgacc acgcttgcga tttcctcgag cagtctcgcg ctgagatgcg ataacgccgc
     7201 cgaggagcgg ctgagattga cgatgtgatc gggccgcatg ccgaagaatt cgtacagcgg
     7261 ccaggccatc tcctcgtgct ggccggtatg caggatcgtc acacccagac gggaggcgcg
     7321 caacgcccgg taaacgggcg ccatcttgat gatctccgga cgcgttccca tgcatatgag
     7381 aacggcgtct tgcttcgccg caacgctcca cataccactt ccctcctgca gccccaccat
     7441 cgttcgcacg acgcggcgtt cgatgtttgc aaccttgcgc gcgactgtga tcgcgtcgtt
     7501 gttcgatcgg acaacggcgt ttcgtttttg caagtgagcg ccgttacatt gcggctgtga
     7561 gcagcctgtg tgccgcaaag cggcagcggc gaatcaacgg tttgttttac aagaacatgc
     7621 aggaaaaata tcgaaccaaa gggaaagcaa cgcgaaatcg cgcgctgtaa cctcgcgcta
     7681 caaagcatcg ttccgaagga cacgcgtatc aagcttcgat ggctacacgt ccgcgtcgat
     7741 acgcgcgtcg atcg
//
LOCUS       DATGOU010000061         4512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_5032865, whole genome shotgun sequence.
ACCESSION   DATGOU010000061 DATGOU010000000
VERSION     DATGOU010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5032865"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VKZ48_03500"
     CDS             complement(<1..285)
                     /locus_tag="VKZ48_03500"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HLU76954.1"
                     /translation="MLKNFLLRMVINAFALAVVVTVLPQHIHIVGSDRQNEMLILLGI
                     ALIFGLVNAIVKPILTFLTCPFILFTLGLFLLVINGLMFMLTAELSRLVLG"
     gene            complement(346..3840)
                     /locus_tag="VKZ48_03505"
     CDS             complement(346..3840)
                     /locus_tag="VKZ48_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HLU76955.1"
                     /translation="MKKSFILLFTLIFILSAVHGAMQDNPAPRVEITGVNATDLPTVV
                     INATVFDSIGQPVRGLSETNFEIVGDLAPQARIVRVENITDDELDISVVLVIDVSSSM
                     EGQPIERAIEAAQLFVDSLGPNDSVAIYTFSHRFNLVQDYTTNKALLNAAIESISAGG
                     RTGLYQAAYNAVDLAAASPSSRRAVILLSDGAEYGGVSQVGRDAALQEARLRGVPFYT
                     IGLGYGIDRTYLQALSEGTNARFVESPTPEELAGIYADLAALLQSQYILTLELDVPAD
                     GTEYDLVLQATTPEGTSLDNATLRAPIPVPIIRPPLLPEVINQVTEVTPDIAADQTIS
                     EVEVTIGEGGQPQTITEPPFTFIIDPETLTPGTHPLTIAATDEDGDTGSASFDMQIGV
                     LPPRVIVEANLDEPVESVQTVILDISGQTPGASATYRIDDGPSTTVEEAPYAFDIRPM
                     ELTPGEHTLTITATNQGGGVTTLEVPFVVAALPPQITINGLTAGQEVDEVTEIEVNVV
                     ASQTPVTSIDFMINGTALESISIPPDDIIISRATLDPMAFEPGDKRLTVRAENASGVA
                     TTGEILFTVAALPPVTVFQNLSEGEVIEEDRTVGISFISQTPVVRVAYFLDSQALPEQ
                     TRAPWDIELNALELGPGDHSLRVVATTANDMQSSATVNFTVGRGPSLTATALVPTNTP
                     SPTPDIPATRTVVAQATQAAEATIAHHTQVAADATATELAFAATGTAESRATGTAFAQ
                     AEAEAQDTAAVQATQARQAEINAQGTASFRATLEFRATQNAERRSTAEAQAALSAQVT
                     ATAEAEASRIAGTEVALAATESSVTQSAVQTAQAAATATDVVQATVTGEAAQTATAEA
                     QQTATSEAQAAATQAAGATATQMIAAQIVATATGEAQQQATATAEALEAQAARETATA
                     QSAATSTAEARQTQAAERQATATQEARLTQTAQAEQATQTAEAQVRQTATRQAQLIAT
                     VTRIAQLEATETAQAEATMTQDARETLVAQAEINAQGTASAQETRAARMAQATATQNA
                     RQTMTAEAQIELDATATAERAEEESAAATRAAQEATQNAQATANAQATIDTQLTAIAT
                     EEVEPTTIAQAPTEGAPEPTQAAPTATPRGTLVPMEVESETADVNQLIPILVVIVVAV
                     VILLVIYLILRGGRNTRR"
     gene            4084..4377
                     /gene="gatC"
                     /locus_tag="VKZ48_03510"
     CDS             4084..4377
                     /gene="gatC"
                     /locus_tag="VKZ48_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HLU76956.1"
                     /translation="MELSHEEVRRIAELAKLDLTDDEVALYAGQLSSVLQYFQRLQEV
                     DTSHVEPTASVLPLSNVFREDIAETPLDPDEVIRNAPDAVDHQFRVSAVLGDE"
BASE COUNT          719 a         1375 c         1469 g          949 t
ORIGIN      
        1 gcccagcacc agccgcgaca gttcggcggt gagcatgaac atcaggccgt tgataaccag
       61 caggaacagg ccgagcgtga acaggatgaa cgggcaggtg aggaacgtca gaatcggttt
      121 aacgatggca ttgaccagac cgaagatcag cgcaatgccc agcaggatca gcatttcgtt
      181 ctgccggtcg gagccgacga tatggatgtg ctgcggaagc accgtgacga cgaccgccag
      241 cgcaaaggcg ttgatgacca tccgcagcag aaaattcttg agcatacttt ccctcgcgcg
      301 atgaacgagg ggcgcagcag ttagccgcgc ccctcgtcgt tagcttcagc ggcgagtgtt
      361 tctgccgccg cgcaagatca gataaatcac cagcaggata acgaccgcga cgacgatcac
      421 caccagtatc gggatcaact ggttgacgtc cgccgtctcg ctctcgactt ccatcggcac
      481 gagcgtgccg cgcggcgtcg ccgtcggcgc ggcctgcgtc ggctccggcg ctccttcagt
      541 cggggcctgc gcgatggtcg tcggttcgac ttcttcggtc gcgatggccg tcaactgcgt
      601 gtcgatggta gcctgcgcgt tcgcggtagc ctgtgcattt tgcgtcgctt cctgagcggc
      661 ccgcgtcgcg gctgcgcttt cttcctctgc gcgttcggcg gtcgccgtcg catcgagttc
      721 gatctgcgct tccgccgtca tcgtctggcg ggcattctgc gtcgccgtgg cctgcgccat
      781 gcgggcggcg cgtgtctcct gagcggacgc tgtgccttgc gcattgattt cagcctgagc
      841 caccagcgtt tcacgagcgt cctgggtcat ggtcgcctcg gcctgcgcgg tctcggtggc
      901 ttccagttgc gcgatgcgcg tgaccgtggc gatcagttgt gcctgccggg tggccgtctg
      961 gcgtacctga gcctcggcgg tctgggtggc ctgttcggcc tgcgcggtct gcgtcagccg
     1021 ggcttcctgt gttgccgtcg cctggcgctc cgcggcctgc gtctggcgag cttcggcggt
     1081 acttgtcgcc gcgctctgcg ccgttgccgt ttcgcgggcg gcctgtgcct ccagcgcctc
     1141 ggcggtggca gtggcctgct gctgtgcttc cccggtcgca gtcgcgacta tttgcgcggc
     1201 gatcatctga gtggcggttg cgcccgcggc ttgcgtcgcc gcggcctgtg cctcactcgt
     1261 cgccgtttgc tgggcttcgg cggtcgcggt ctgcgccgct tcacctgtga cggtcgcttg
     1321 cacaacgtcc gtggcagttg cggcagcctg cgccgtctgt actgcggact gcgtgacgct
     1381 gctttcagtc gccgccagcg ccacttccgt gccggcgatg cgcgatgctt cggcctcggc
     1441 agtagctgtt acctgcgcgc tcagtgccgc ctgtgcttcg gcggtgctcc ggcgctcggc
     1501 gttctgagtg gcgcggaatt caagcgtcgc ccggaacgaa gccgtacctt gcgcgttgat
     1561 ttcagcctgt cgcgcctgag tcgcctgcac agcggcagtg tcttgtgctt cggcttcggc
     1621 ctgtgcgaag gccgtacctg tggcccggct ttccgccgtg ccggtggcag cgaacgcgag
     1681 ttcggtagcg gtggcgtcag ccgcaacctg cgtgtgatgg gcaatcgtgg cttcggcggc
     1741 ctgagtcgcc tgcgcgacta ccgtgcgcgt cgccgggata tcgggcgtcg gcgagggcgt
     1801 attggtcggc acaagcgcgg tcgcagtcag cgaaggcccg cgaccaaccg taaagttcac
     1861 cgtcgcgctc gactgcatgt cattcgcggt tgtcgcgacg acgcgcaggc tgtgatcgcc
     1921 cggccccagt tccagcgcgt tgagttcgat gtcccacggc gcgcgcgttt gctccggcag
     1981 ggcttggctg tcgaggaaat aggccacgcg gacaaccggc gtctggctga tgaacgagat
     2041 gccgacggtg cgatcttctt cgatcacttc gccttcgctc agattctgga agacggttac
     2101 cggcggcagc gcagctaccg tgaacaggat ttcacccgtc gtggcgacgc cgctggcgtt
     2161 ttcggctcgc accgtcagcc gcttgtcgcc cggctcgaat gccatcggat caagcgtcgc
     2221 ccggcttatg atgatgtcat ccggcggaat ggaaatgctt tccagcgccg tgccgttgat
     2281 catgaagtcg atgctggtca ccggcgtctg gctggcgacg acgttcacct cgatctcggt
     2341 gacttcgtca acttcctgcc cggcggtcag gccgttgatc gtgatctgcg gcggcagcgc
     2401 cgcgacaaca aacggcactt ccagtgtggt tacgccacca ccctgattag tcgccgtgat
     2461 ggtcagcgtg tgctcgcccg gcgtcaactc cattggccgg atatcgaagg cgtagggcgc
     2521 ttcttctact gtggtcgagg gaccatcgtc aatccggtag gtagcgctgg ctccgggcgt
     2581 ctggccgctg atgtcgagga tgaccgtctg cacgctttcg acaggttcgt cgagattcgc
     2641 ttcgacgatc acgcgcggcg gcagcacgcc gatctgcatg tcaaacgagg cagacccggt
     2701 atcgccgtct tcgtcggtgg cggcaatggt gagcggatgc gttcccggcg tcagcgtttc
     2761 ggggtcgatg atgaaggtaa acggcggctc ggtgatggtc tgtggctggc cgccctcgcc
     2821 gatggtcact tcgacctcgc tgatcgtctg gtcggcggcg atgtctggcg tgacttcggt
     2881 gacctgattg atcacttccg gcagcagcgg tggccggata atcggcaccg gaatcggcgc
     2941 gcgcagcgtc gcgttatcaa gcgacgtgcc ttctggcgtg gtcgcctgaa ggaccagatc
     3001 gtattccgtg ccgtcggcgg gaacatccag ttccagcgtc aggatgtact ggctttgcag
     3061 caacgctgcc agatcggcgt agataccggc aagctcttcc ggcgttggcg attcgacaaa
     3121 acgggcgttc gtaccttcag acagcgcttg cagatacgtg cggtcgatgc catagccgag
     3181 gccgatggtg tagaacggca caccgcgcag ccgcgcctcc tgcagcgccg catcgcgccc
     3241 gacctggctg acgccgccgt attccgcgcc gtcgctgagc aaaatcacgg cccggcggct
     3301 cgacggcgag gcggcggcca gatcgaccgc gttgtaggct gcctgataaa gcccggttcg
     3361 accaccggcg ctgatggact cgatggcggc attgagcagg gctttgttgg tcgtgtagtc
     3421 ctgtaccaga ttgaagcggt gcgagaaagt atagatcgca accgaatcgt tggggccaag
     3481 cgagtcgacg aacagttgcg cggcctcgat agcgcgctcg atgggctggc cctccatgct
     3541 actgctgacg tcaatgacca gcaccaccga gatatcaagt tcgtcgtcgg tgatattttc
     3601 gacgcgcaca atccgcgcct gcggcgccag atcgccaaca atctcgaaat tggtttcgct
     3661 gaggccgcgc accggctggc caatggaatc gaacaccgtc gcattgatca ccaccgtggg
     3721 caggtcggtg gcgttaacgc cggtgatctc cacgcgggga gcggggttgt cctgcatggc
     3781 cccgtgtacg gctgacagga tgaaaatcag tgtgaaaagg aggatgaacg actttttcac
     3841 taaattgctc ccatggggtc gcaaggaaat aagcaagtat aagcgtcgcc tgagcgacgt
     3901 gttttgatcc tgtctaatct taaccccttt cctcgccagc gcaatcagtc tatggtagtt
     3961 cgttgggacg aatattcgat tggattgcaa ttatcgcttc gatggcgacg cgcgcttttg
     4021 ttccggcggc ggcgggcgct ataattccgg cgtcgtggct gaagcgaaag aaggaatcgc
     4081 atcgtggagc tttcacacga agaagtgcgg cggattgccg agttggcgaa gctcgacctg
     4141 acggacgacg aagtcgccct gtatgccggg caactttcca gcgtgcttca gtattttcag
     4201 cgtttgcagg aagtggacac atcgcatgtc gagccaacgg catccgtgct gccgctgagc
     4261 aacgtctttc gcgaggatat tgccgaaacg ccgcttgatc ccgacgaagt catacgtaac
     4321 gcgccggatg cggtcgatca ccagttccgc gtcagcgccg tgcttggcga cgaatagggc
     4381 ggcccggtcg cccggatatg cgccaacatt tctaagcgca ttgcgcttca ttcgcgcgtc
     4441 cggtatagtc tccgttgctt ctttatttca aaggggataa caggatgcag cgatcggaaa
     4501 gcggccacgc ca
//
LOCUS       DATGOU010000062         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_4594668, whole genome shotgun sequence.
ACCESSION   DATGOU010000062 DATGOU010000000
VERSION     DATGOU010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4594668"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VKZ48_03515"
     CDS             complement(<1..410)
                     /locus_tag="VKZ48_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLU76957.1"
                     /translation="MIPRTTLEQWRVLQAIVEEGSYAKAAEALHRSQSSVSYMVAKLQ
                     EQLGVDLLAIEGRKAQLTPQGAVLLNRAKELLIDAQRLEQLAGSLRIGREPEVRLATD
                     VVFPNQVLLDALRRFADIAPTTRVQLREVVLSGA"
     gene            513..1115
                     /locus_tag="VKZ48_03520"
     CDS             513..1115
                     /locus_tag="VKZ48_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012363006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HLU76958.1"
                     /translation="MTTILHINSSARTNGTANQLAGTFVSEWTAQHPDHRIVQRNLTI
                     DPIPHLAEETINAFFTPAEQRTEAQREAVRLSDELIAELFAADVIVIGVPMYNFGIPS
                     TLKAYFDHIARAGITFRYTSNGAEGLVKGKKAYIVAARGGVYSGTPADTQTAYLKTFL
                     GFIGVTDVTFIYAEGLNLGEEAKARGEEQAQRCIRELAAA"
     gene            1128..1826
                     /locus_tag="VKZ48_03525"
     CDS             1128..1826
                     /locus_tag="VKZ48_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HLU76959.1"
                     /translation="MIEIRKAQTRGHAQHGWLESWHSFSFAEYYDPKQMGWGPLRVIN
                     DDTVRGGGGFPPHGHNNMEIISYVLDGALAHKDSTGGSSTIRPGDVQVMSAGTGVVHS
                     EFNASATDPVHFLQIWITPERTGVEPAYRERHFDATEKRGRLRVIASPDERDGSLKIW
                     QDACVYAALLDGDERIDRPIEAGRRAYVHVARGAVTLNGEQLSAGDGVKLAGKTVVQL
                     SGGRGAEVLLFDLP"
     gene            1859..2047
                     /locus_tag="VKZ48_03530"
     CDS             1859..2047
                     /locus_tag="VKZ48_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76960.1"
                     /translation="MTTQPKQHRPAPAGEDVVAKHLERELDAALEATFPASDPIAVPS
                     LAVLKARRRQSKPQHRSR"
BASE COUNT          418 a          724 c          627 g          343 t
ORIGIN      
        1 tcggcaccgg acagcacgac ctcgcgcaac tgcacgcgcg tcgtcggcgc gatgtcggca
       61 aagcggcgca acgcatcgag cagcacctga ttgggaaaca cgacgtccgt cgccagccgc
      121 acctcgggct cacgcccgat ccggaggctg ccggccagtt gttcgagccg ctgcgcatcg
      181 atgagcaatt ccttcgcacg gttgagcagc acggcgccct gcggcgtgag ctgcgccttg
      241 cgcccctcga tcgcgagcag gtcgacaccc agttgctcct gcagtttcgc caccatgtag
      301 ctcaccgaag actgactgcg atgcagcgcc tcggccgcct tggcatagct gccttcctct
      361 acgatggcct gcagcacccg ccactgctcc agcgtcgttc tggggatcat tgatcgggtt
      421 ttccaattaa attgcccgat actttgcgct tttttatcta tttttacaaa gggagaatgg
      481 gtcgtcccaa tcatttaccc aggagcaccc cgatgacgac cattctgcac atcaactcca
      541 gcgcgcggac caacggcacc gccaaccaac tggccggcac cttcgtaagc gagtggaccg
      601 cgcagcatcc ggaccaccgc atcgtgcagc gcaacctcac gatcgatccg attccccacc
      661 tggcggagga gacgatcaac gccttcttca cgccagccga acagcgcacc gaggcacaac
      721 gcgaagccgt acgcctgtcc gacgaactga tcgctgaatt gtttgccgcc gacgtcatcg
      781 tgatcggcgt gccgatgtac aacttcggca tcccctccac gctcaaggcc tatttcgacc
      841 acattgcccg cgccggcatc accttccgct acacctcgaa cggcgccgag ggactcgtca
      901 aaggcaagaa ggcctacatc gtcgcggcgc gcggcggcgt ctacagcggc acgccggcgg
      961 acacgcagac ggcgtacctc aaaacctttc tgggcttcat cggcgtcacg gacgtgactt
     1021 tcatctacgc cgaaggactg aacctgggcg aggaagcgaa ggcgcgcggc gaagagcagg
     1081 cgcagcgctg cattcgcgaa ctcgccgcgg cttgagggag cacgctcatg atcgaaattc
     1141 gcaaagcgca aacgcgcgga cacgcgcaac acggctggct ggaaagctgg cacagcttct
     1201 ccttcgccga gtattacgat ccgaagcaga tgggctgggg gccgctgcgc gtcatcaacg
     1261 acgatacggt gcgaggcggc ggcgggttcc ccccgcacgg acacaacaac atggagatca
     1321 tttcctatgt gctcgacggc gcgctggcac acaaggattc gaccggcggc agttcgacca
     1381 tccggccggg cgatgtgcag gtcatgagtg ccggcacagg cgtggtccat agcgagttca
     1441 acgcatccgc caccgatccg gtgcacttcc tgcagatctg gatcacaccc gaacgcaccg
     1501 gcgtcgagcc ggcctatcgc gaacgccatt tcgacgcgac ggaaaagcgc ggccggctgc
     1561 gcgtcatcgc ctcgcccgac gaacgcgacg gctcactgaa gatatggcag gatgcctgcg
     1621 tctatgccgc cctgctcgac ggcgacgaac gcatcgatcg cccgatcgaa gccggccggc
     1681 gcgcttatgt ccatgtggcg cggggcgcgg tcacgctgaa cggcgagcag ttgtccgcag
     1741 gcgacggcgt gaaattggcc ggcaaaaccg ttgtccaact gagcggcggg cgcggggcgg
     1801 aagtgttgct cttcgacctg ccctgacgtt ccttgtgcat tccccagcag gagtctcgat
     1861 gacgacgcaa cccaaacagc accgccccgc acccgccggc gaggacgtcg tggccaaaca
     1921 tctggagcgc gaactggatg ccgcgctcga agccaccttt ccggccagcg accccatcgc
     1981 ggtgccgagc ctggcggtgc tgaaggcgcg tcgccgtcag tccaagccgc aacaccgctc
     2041 gcgatagcga ccagccagaa accgggcggc ccggctcctc acaacggcgc cgaagttttt
     2101 tacaaacagc ct
//
LOCUS       DATGOU010000063         6076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_1945125, whole genome shotgun sequence.
ACCESSION   DATGOU010000063 DATGOU010000000
VERSION     DATGOU010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6076
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1945125"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..486)
                     /locus_tag="VKZ48_03535"
     CDS             complement(67..486)
                     /locus_tag="VKZ48_03535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HLU76961.1"
                     /translation="MNHQDTKAPRKTDSIGVEVDAVARAIVDSAYRIHKTLGPGLLES
                     AYESCFHIELSRRGLPFQSQLRLPITYEGIVVEPAYRLDLVVDNAVIVEIKATEKLMP
                     VHEAQLLTYLKLTGLRVGLLINFNASLIKDGIKRFVL"
     gene            complement(483..1964)
                     /gene="trpE"
                     /locus_tag="VKZ48_03540"
     CDS             complement(483..1964)
                     /gene="trpE"
                     /locus_tag="VKZ48_03540"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HLU76962.1"
                     /translation="MTEAEFKRLAAEGYNRIPLVLETFADLDTPLSIYLKLANRPHTY
                     LLESVVGGERFGRYSFVGLAASTRLEIVGQQVRIHNGDAVVERVETADPLQYVRQYLS
                     RFKAAERAGLPRFCGGLVGYFGYDTVRWIEPRLQGGWSKPDPIGSPDALLLLSEELAV
                     VDNLSGRLYLVVYGDPTRPNGYEAARARLRALAEGLKQPLQLPDNGAHAPGVVESNFG
                     EEAFKAAVARAKQYIHDGDVMQVVLSQRMSQRYEASPLSLYRALRALNPSPYMFYFDL
                     GEQYIVGASPEILVRLENGNVTVRPIAGTRPRGKTVEEDIGLAEDLRADPKERAEHVM
                     LVDLGRNDVGRVAQTGTVKLTESMAIERYSHVMHIVSNVEGKLKEGLDAIDVMRAAFP
                     AGTVSGAPKVRAMEIIDELEPVKRGVYAGAVGYFGFNGDMDMAIAIRTALIKDRTLHV
                     QAGAGIVADSVPESEWVETCNKARALLRAAEIAAAGLNGEQKS"
     gene            complement(2178..2849)
                     /locus_tag="VKZ48_03545"
     CDS             complement(2178..2849)
                     /locus_tag="VKZ48_03545"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013391098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HLU76963.1"
                     /translation="MSGPYRAALCDLDGTLLDTAVDIWRAANRMQETLGRPPVSAEDI
                     GRYIGKGIPILVHRVLTGELDGRAEASLFDRALALFEQAYAEESGRAAVLYPNVLEGL
                     QRIRAAGIRLACVTNKAGRYTGDLLAQAGLAEFFQVVVSGDTLPVKKPDPRPLLHACE
                     QLGVAPAHAVVIGDSMNDVQAGRNAGLYVLAVPYGYNEGRPANELGADRVVADIAEAA
                     DFLGA"
     gene            complement(2846..3520)
                     /gene="rpe"
                     /locus_tag="VKZ48_03550"
     CDS             complement(2846..3520)
                     /gene="rpe"
                     /locus_tag="VKZ48_03550"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HLU76964.1"
                     /translation="MSANRYRIAPSILSADFARLGEEVKAVVAAGADFIHFDVMDNHY
                     VPNLTVGPMVCEAIRPITEATIDVHLMVKPVDRIIPDFAKAGANIISFHPEASEHVDR
                     TIGLIKDAGCKAGLVFNPATPLSWLDYTLQSIDLVLIMSVNPGFGGQKFIPGALDKLR
                     EVRRRIDASGRDIMLEIDGGVKVDNIAEIARAGACTFVAGSAIFNTPDYKATIEAMRA
                     QLATVA"
     gene            3627..3908
                     /locus_tag="VKZ48_03555"
     CDS             3627..3908
                     /locus_tag="VKZ48_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76965.1"
                     /translation="MTQDPQISAKSALQTIQQRYARIAEEILDRWGTDRLEPYIDSLL
                     IDDRGNRSGFPPEVAQALLMLSSEHSRRYPPAASADPWHWTDPTTAKPR"
     gene            3969..4400
                     /gene="apaG"
                     /locus_tag="VKZ48_03560"
     CDS             3969..4400
                     /gene="apaG"
                     /locus_tag="VKZ48_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HLU76966.1"
                     /translation="MRPAQTRAADLILVPWNAVDPKYAFNIEVETQYLPEQSDESADR
                     YVFAYTITVTNVGEVTAQLISRHWIITDGNHDVQEVRGLGVVGEQPTLEPGQRFQYTS
                     GASLATPVGTMRGSYQMVAADGTRFDAPIPEFTLSIPRVLH"
     gene            4726..5802
                     /locus_tag="VKZ48_03565"
     CDS             4726..5802
                     /locus_tag="VKZ48_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HLU76967.1"
                     /translation="MMEQPAAAPLAPATFADLEGWNADAVEQAWSAFLTSCTTLVNRP
                     EWQAVCTEASTMSAEPAAIRAFFESRFLPFQLTNPDGSVTGLITGYYEPLLRGSRTRT
                     GEYKIPLYAAPEDLLIIDLSTVAPDTKHMRLRGRLEGRKVVPYYSRAEIDQGAAPVAG
                     KEIVWVDDAVEAFFLQIQGSGRVQLPDGSMVRVGYADQNGHPYKSIGRVLIERGELQL
                     HEASMQGIQRWARTNPDKLAELLAQNPSYVFFRELPPRDGGPIGALGVPLTPERSIAV
                     DPRHVPLGAPVFLATTRPNSDVPLQRLMVAQDTGGAIRGVVRADFYWGFGPAAGAEAG
                     RMRQQGRMWVLLPRDHPQARAATN"
     gene            complement(5816..>6076)
                     /locus_tag="VKZ48_03570"
     CDS             complement(5816..>6076)
                     /locus_tag="VKZ48_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HLU76968.1"
                     /translation="DSMAKSQKVWCSPDRAKAWQGWMLEGKAPAGEGNCETPILNNLQ
                     LGQQLRIRGTPALFFPNGQRVSGAITADEVRDLLTRAAAKTK"
BASE COUNT         1103 a         2015 c         1879 g         1079 t
ORIGIN      
        1 gtcgatcatg agcaacatcg ttaaacctca attcattgaa ccaccaaggc accaagacac
       61 caagaactac aggacgaatc gcttgatacc gtccttgatg agcgaggcat tgaagttgat
      121 cagaagaccc actcgcaagc ctgtcagctt caagtatgta agaagctgcg cttcatgcac
      181 cggcatcaac ttctccgtgg ccttgatttc cacgattaca gcgttgtcga ccaccaggtc
      241 caatcgatac gcgggttcaa cgacaattcc ctcgtaggtg atcggcaagc gcaattgcga
      301 ctgaaagggc aaaccacgtc ggctcagttc aatatgaaaa catgattcat aggccgattc
      361 gagcagcccc gggccgagtg tcttgtggat ccgatacgcc gaatcaacga tagcgcgggc
      421 aaccgcatcc acctcgacac caatgctgtc cgtttttctt ggtgccttgg tgtcttggtg
      481 gttcacgatt tttgttcgcc gttcaaaccg gcggccgcga tttccgccgc gcgcagcagc
      541 gcgcgcgcct tgttgcaggt ctcgacccac tcgctttccg gcaccgagtc ggcgacgatg
      601 ccggccccgg cctgcacgtg cagcgtccga tccttgatca gcgccgtgcg aatggcgatg
      661 gccatatcca tatcgccgtt gaaaccgaag tagccgacgg cgccggcata gacgccgcgc
      721 ttcaccggct ccagttcgtc gatgatttcc atcgcccgca ctttcggcgc gccgctcacc
      781 gtaccggcgg ggaaagcggc gcgcatgaca tcgatggcgt cgaggccctc tttcagcttt
      841 ccttccacgt tggagacgat gtgcatcaca tgcgagtagc gctcgatggc catgctttcg
      901 gtcaacttca cggtgcccgt ttgcgcaacc cggccgacat cgttacgccc caggtcgacc
      961 agcatgacgt gttcggcgcg ttccttgggg tcggcgcgca aatcctcggc caggccgata
     1021 tcttcttcga ccgtcttgcc gcgcggccgc gttcccgcaa tggggcgaac agtcacgtta
     1081 ccgttttcca gccgtaccag gatttccggc gaggcaccga cgatgtactg ctcgccgaga
     1141 tcgaaataga acatgtaggg cgagggattg agcgcccgca gtgcccggta cagcgacagc
     1201 ggcgaagctt catagcgctg actcatgcgc tgcgacagta cgacctgcat gacgtcgccg
     1261 tcgtgaatgt actgcttggc tcgtgcgacg gccgccttga acgcctcctc gccgaagttc
     1321 gactcgacga cgccgggcgc atgcgcgccg ttgtccggca gttgcagcgg ctgtttcaga
     1381 ccttccgcca gggcgcgcag acgtgcacgg gccgcctcgt agccgttcgg gcgagtcgga
     1441 tcgccataga ccaccaggta cagcctgccg ctcaggttgt cgacgaccgc cagttcttcc
     1501 gaaagcagca gcagcgcgtc gggactgcca atcggatcgg gcttggacca gccgccctgc
     1561 agccgcggct cgatccagcg cacggtgtcg taaccgaagt agcccaccag accgccgcag
     1621 aagcgcggca gcccggcgcg ttccgccgcc ttgaagcggc tgaggtactg ccgcacatac
     1681 tgcagcggat cggcggtctc cacgcgctcg accaccgcat caccgttatg gatgcgcacc
     1741 tgctgcccga cgatctccag ccgcgtggac gccgccaagc cgacgaagga atagcgcccg
     1801 aagcgctcgc cgccgaccac ggactcgagc agataggtgt gcggccggtt ggccagcttg
     1861 aggtagatcg ataagggtgt atcgaggtcg gcgaaggtct ccagcaccag cgggatgcgg
     1921 ttgtaacctt cggcggcaag ccgcttgaat tcggcttcgg tcataagctg ctccagaaaa
     1981 accttcggac gatctgcggc gcgctacgca tcgccggcgc gcacggcgta ccacggagac
     2041 atcagcgcca gcggcgccag tgctcccggt cgcgttgcca cagaaggttg aagatcgggc
     2101 agctcacgtt ttccagagac ggatcagata cctgatgcgg ccgactatag cattccgact
     2161 cgtgccgcgt cacccggcta ggcgccgagg aagtccgcgg cttcggcgat atcggccacg
     2221 acgcgatcgg cgcccagttc gttcgccggg cggccctcgt tgtagccata gggcacggcc
     2281 agcacataca agccggcgtt gcgaccggcc tggacatcgt tcatggaatc gccgatcacg
     2341 acggcatgcg ccggcgcgac accgagttgc tcgcaggcgt gcagcagcgg acgcggatcg
     2401 ggtttcttca ccggcagcgt gtcgccgctg accacgacct ggaagaattc cgccaatccc
     2461 gcctgtgcca ggagatcgcc ggtgtagcga cccgccttgt tggtcacgca ggcgagccga
     2521 atgccggccg cgcggatacg ctgcaggcct tccagcacat tcggatagag cacggcggcg
     2581 cgccccgatt cctcggcata ggcctgctcg aacaatgcca gggcgcgatc gaacagcgac
     2641 gcctccgcac gaccgtcgag ttcgccggtc agtacgcgat gaacgaggat gggaatgccc
     2701 ttgccgatat agcggccgat atcctcggcc gaaaccggcg gacggccgag cgtctcctgc
     2761 atgcggttcg ccgcgcgcca gatatccacg gccgtgtcga gcagcgtgcc gtcgaggtcg
     2821 cacagcgcag cgcgatacgg cccgctcatg ccacggtagc gagctgggcc cgcatcgcct
     2881 cgatggttgc cttgtagtcc ggcgtgttga aaatggccga gcccgcgacg aaggtgcagg
     2941 cgccggcgcg cgcgatctcg gcgatgttgt ccactttcac gccgccgtcg atctcgagca
     3001 tgatgtcgcg cccgctggca tcgatgcgac gacggacttc gcgcagctta tcgagcgcgc
     3061 cgggaatgaa cttctgtccg ccgaaacccg ggttgaccga catgatgagc accagatcga
     3121 tggactgcag cgtgtaatcg agccagctga gcggtgtcgc cgggttgaag accagtcccg
     3181 ccttgcagcc ggcgtccttg atcaggccga tggtgcggtc gacatgctcg ctcgcctcag
     3241 ggtgaaagct gatgatgttt gcgcccgcct tggcgaaatc gggaatgatg cgatcaaccg
     3301 gtttcaccat gaggtggacg tcgatcgtcg cctccgtgat cggccggatc gcttcacaga
     3361 ccatcgggcc gacggtcagg ttgggcacgt agtggttgtc catgacgtcg aagtgaatga
     3421 aatcggcgcc ggcggcgacg acggccttca cttcctcgcc caggcgggcg aagtcggcgg
     3481 acaggatgct gggtgcgatc cgataacgat tggcggacat gacgcggcga tcaaaacaac
     3541 aagaggccga ttctacatct ccgcgcgccg gttccggccg actcgggtat gctgccggca
     3601 tgaacagccc ttgggggagg gatgtcatga cgcaggaccc gcagatttcg gccaagtccg
     3661 cgctgcagac gatccagcag cgttacgcgc gcatcgccga ggaaatcctc gatcgctggg
     3721 gcacggatcg actcgaaccc tatatcgaca gcctgctgat cgacgatcgc ggcaatcgca
     3781 gcggctttcc gcccgaggtc gcccaggcgt tgctcatgct ctcgagcgaa cacagtcgcc
     3841 gctatccgcc cgccgcgtcg gccgatccgt ggcactggac ggatccgacg accgccaaac
     3901 cccgctagca ggggtcgcgc ggcgagttgt atgcgctgcg gaattgttgt gcaattggga
     3961 gcctctccat gcggcctgcg cagacgcgtg cggccgacct cattctcgta ccgtggaacg
     4021 ccgtggaccc caaatacgcc ttcaacatcg aagtcgagac gcagtatcta cccgagcagt
     4081 cggatgaaag cgccgatcgc tatgtcttcg cctacaccat taccgtcacg aacgtcggcg
     4141 aagtcacggc gcagctcatc agccgccatt ggatcatcac cgacggcaac cacgacgtgc
     4201 aggaagtgcg cggcctcggc gtggtcggcg aacaacccac cctcgaaccg ggacagcgct
     4261 ttcagtacac cagcggcgcg tcgctggcca cgcctgtggg cacgatgcgc ggcagctatc
     4321 agatggtcgc cgccgacggc acgcgcttcg acgcgccgat tcccgaattc accctgtcga
     4381 ttccgcgcgt gctccattga gccgcgcgat cgcgcatctc gtcgtccgcc cggtcaatgc
     4441 ggatcgtcgt cgcgcttatt acgcttcggc aacgtccacc agacgatgaa caccaacagc
     4501 gccaatgcca ccgccgcctg aagaatgagt atccacagcc cgctatccat cgttccgccg
     4561 ttcccccgtt tgactgcaag tgcacgcgca atgatagtcc gcggcgtcgc ctttctcgcc
     4621 tttgccgcgc tactggccgg ctgcgcgaca aagccggcgc ctgagcaagc acggactccg
     4681 acttgcccgg ccgcaacctg ccccgcgtgt ccggtatgcc cggacatgat ggagcagccc
     4741 gccgccgcac cgcttgcccc ggcgacattc gccgacctcg aaggctggaa tgccgatgca
     4801 gtggaacagg cgtggagcgc ctttctgacc agttgcacga cgctcgtcaa ccgcccggaa
     4861 tggcaggcgg tctgtaccga ggcttcgacg atgagcgccg aacctgccgc catacgcgca
     4921 ttcttcgaat cgcgctttct gccctttcaa ctcaccaacc ccgacggcag cgtcacaggc
     4981 ctcatcaccg gctactacga accgctgttg cgcggcagcc gtacgcgtac cggcgaatac
     5041 aagattccgc tctacgcggc gcccgaggat ctgctgatca tcgatctcag cacggttgcg
     5101 cccgatacca agcacatgcg actgcgcggc cggttggaag gccgcaaagt ggtgccttat
     5161 tacagccgcg cggagatcga tcagggtgcc gcacccgtcg ccggcaaaga aatcgtctgg
     5221 gtggacgacg cggtggaagc cttttttctg caaatccagg gttccgggcg cgtgcagttg
     5281 cccgacggca gcatggtgcg cgtgggctac gccgatcaga acgggcatcc ctacaaatcc
     5341 atcggtcggg tgctgatcga gcgcggggaa ttgcaactgc acgaggcgtc gatgcagggc
     5401 atccagcgtt gggcgcgcac gaatccggac aagctggccg aattgctcgc ccagaatccc
     5461 agctacgttt ttttccgcga actgccgccg cgcgacggcg ggccgatcgg cgcgctgggc
     5521 gtgccgctca ccccggagcg ctcgatcgcc gtcgatccgc gtcacgtgcc gctcggcgcg
     5581 ccggtttttc tggcgaccac gcgcccgaat tcggacgtgc cgctgcaacg cctgatggtc
     5641 gcgcaggaca cgggcggcgc gattcgcggt gtggtccgcg ccgacttcta ctggggtttc
     5701 gggccggcgg ccggcgccga ggccggccgc atgcggcagc agggccggat gtgggtgctg
     5761 ctgccccggg atcacccgca ggcgcgggcg gcgacgaact gagtccgagc ctcgcttact
     5821 ttgtcttggc ggccgcccgc gtcagcaggt cgcgcacttc gtccgccgtg atggcacctg
     5881 agacgcgctg accgttgggg aagaacaggg ccggcgttcc gcgaatgcgc aactgctgtc
     5941 ccaactggag attgttcaag atcggcgtct cgcagttgcc ctcgcccgcc ggcgccttgc
     6001 cttccagcat ccatccctgc caggccttgg cacggtcggg cgagcaccac accttctgcg
     6061 atttcgccat ggaatc
//
LOCUS       DATGOU010000064         9314 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_1494988, whole genome shotgun sequence.
ACCESSION   DATGOU010000064 DATGOU010000000
VERSION     DATGOU010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9314)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9314)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9314
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494988"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VKZ48_03575"
     CDS             complement(<1..790)
                     /locus_tag="VKZ48_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HLU76969.1"
                     /translation="MDVRAAVCYAPGEPLVVETVQLDGPKEGEVLVEIKATGICHTDE
                     FTRSGADPEGLFPAIFGHEGAGIVVDVGSGVTSLKKGDHVIPLYTPECRQCKSCLSQK
                     TNLCTAIRATQGQGVMPEIALAKIREDAPFDKVCYIGCGVTTGIGAVIHTAKVEPGAN
                     VVVFGLGGIGLNVIQGARMVGADMIVGVDINPEREALARKFGMTHFINPKEIQGDLVP
                     YIIELTKGGADYSFECVGSVELMRQALECCHRGWGQSIIIGVAGA"
     gene            963..1652
                     /locus_tag="VKZ48_03580"
     CDS             963..1652
                     /locus_tag="VKZ48_03580"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HLU76970.1"
                     /translation="MTTETEIPASAYAEQRARLLIDIAGMAAYSASYTGRSSITPRVM
                     KAIERVPRHAFVPESQRAHAYENRPLPIGEGQTISQPYIVALMTDLLELEPDDVVLEV
                     GTGSGYQAAVLAELAAQVYTIEVIESIGRRGAEVLRELGYTNVHTRIGDGYAGWPEHA
                     PFDAIIVTAAARHVPQPLIDQLKPGGRLVIPVGPEGGMQQLLLLRKQRDGSVTQTDTI
                     PVQFVPLTRSP"
     gene            1718..3580
                     /gene="pabB"
                     /locus_tag="VKZ48_03585"
     CDS             1718..3580
                     /gene="pabB"
                     /locus_tag="VKZ48_03585"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HLU76971.1"
                     /translation="MSASATHSPDPSLLSQCDAASAWALVDGAAPDSDAQGVWVCTGL
                     LERIEAHVPDAVSDACARIEAAGRTHPVVALLDYELGYWLEPRAALRPPEVVRAPLTA
                     LVFEHATWLPHSAFDEELARAVAALPEEARHCGIADLTRSCDRESYRAAIERILQYIH
                     EGDCYQINYTWPMHFRCYGSPLALYLALRKRQPVTHGALVQLPDRTVYSLSPELFLER
                     HGTRLHSRPMKGTAPRGTTPEEDQRNADALQASEKDRAENVMIVDLIRNDLGRVASPG
                     SVRVASLFDIERYPTVLQMVSSVVADAPDASLHEILHALFPCGSITGAPKIRAMQIAQ
                     ELERGPRQLYTGSIGLIRPGGDFSFSVVIRTLEVAADGTGRLGIGSGIVADSDPDREY
                     DECLAKARFATDLPADFELIETLRLEPQADDPYPLLPRHLERLSASARHFGFAYDEGR
                     VRAALDATRRQAGEAPHRVRLTLAKSGEITVRSVPFAAAAGTPGVVYAEAAVHTANPF
                     LHHKTTVRGQYDAALKAIEALPGCFDALFFNERGELTEGARSNVYLVRDGKWYTPPLS
                     SGVLNGVMRRVLMETHTPRIEERVLRREDVEAAEEIHLSNALRGLFRVRLMSRG"
     gene            complement(3596..3970)
                     /locus_tag="VKZ48_03590"
     CDS             complement(3596..3970)
                     /locus_tag="VKZ48_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76972.1"
                     /translation="MNALRPRTRRRLILLGAAALLCGFSTAVAHADSGAVRMLVWAGA
                     HPYYYDSCYLYGRCSADDLRRLRDRLQRLDRVAPQPPEPPPPAAPAARVNVPPTPESH
                     IHPEFRNSSQLREEWRDKSSLK"
     gene            complement(3995..4177)
                     /locus_tag="VKZ48_03595"
     CDS             complement(3995..4177)
                     /locus_tag="VKZ48_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76973.1"
                     /translation="MESINTLRSNGTGFLHLSRRLQPGETVPHIPSLADQLLACRLVR
                     QEQPEPVPDEALEEDF"
     gene            complement(4540..5100)
                     /gene="efp"
                     /locus_tag="VKZ48_03600"
     CDS             complement(4540..5100)
                     /gene="efp"
                     /locus_tag="VKZ48_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HLU76974.1"
                     /translation="MKIAQELRVGNVVMLGNDPMVVQKAEFSKSGRNASVVKMKFKNL
                     LSGAAAENVYRADEKFDVVVLDRKEVTYSYFADPMYVFMDSEYNQYEVEKDNMGDALN
                     YLEDGMECEVVFYNDKAISVELPQTVVRKIDYTEPAVRGDTSGKVLKPAKLATGFEIA
                     VPLFCETGDLIEIDSRTGEYRRRANG"
     gene            complement(5180..6322)
                     /gene="earP"
                     /locus_tag="VKZ48_03605"
     CDS             complement(5180..6322)
                     /gene="earP"
                     /locus_tag="VKZ48_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HLU76975.1"
                     /translation="MWDLFCSVIDNYGDAGVSWRLARTLRRDHGIPVRLWLDDLRTLS
                     QLAPAVDANAAVQTVEGVEIRAWSEPFPQVEPGVVVIEAFGCRLPETFLAAMAVRRPQ
                     PVWLNLEYLSAEDWVRGCHRGPSVHPRLPLHKYFFFPGFEEGTGGLLRERGLIAARRA
                     FQQDAQALATYWQTQGLPLPAPDERRVSLFCYGHAPVGELLDAWRTGSFPVTALVPTG
                     PVVAPIAAYFGCGALRPGECVIRDALRLHVLPFSDQAGYDRLLWACDLNFVRGEDSFV
                     RAQWAARPLVWHIYPQEEGAHQAKLAAFLDRYCAELPAEAAAAQRALHAAWNAVPNAP
                     TVGAAWRQWLAHQDQARAHAVRWAQLRESGPELGSELVKFVQRQVK"
     gene            6728..8167
                     /locus_tag="VKZ48_03610"
     CDS             6728..8167
                     /locus_tag="VKZ48_03610"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HLU76976.1"
                     /translation="MSLHSDALPQRISTGIAGLDSVLGDGLMQHGMYLLEGPPGSGKT
                     ILSAQICFHHAHAQRKTLYVTLIAESHGKLLNNLRAFDFYDPALVPERFILMSGYQEL
                     KTSGLPGLLQLVARALAEHRPSVLVIDGYRSLRMLAPDAATTAEFIYELNTLITTTKS
                     LCLLLAPRDDSGAWAEKTLMDGVIELHRFSVGMRSVREVEVHKLRSSAPIEGRHAFKI
                     CSAGIRVFPRLEASAPFRNAAPDREATRCTFGIEGMDDMLHGGVLRDSATCLLGPPGS
                     GKTIFGLHFLHQGLQAGDTGLHFGFYESPAQLLDKAANLGLDLNEAHRNGQFDILWQP
                     ALECRPDELAMQLLENVWQRKARRVVIDGLEGFMQSAVRPERIGRFFNALVGELRRQQ
                     VACLYTEEVPLFAREVSHGTLATSAIVDNQILLRFVRHEAGMRRTVAVLKQRESGHDH
                     GVREFWIERGGIRVASDSDSAHAFLAERP"
     gene            8164..8571
                     /locus_tag="VKZ48_03615"
     CDS             8164..8571
                     /locus_tag="VKZ48_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLU76977.1"
                     /translation="MIAQTDTDTLTSMKSATKSILVVDDEFDVRAIVELILGMQNYDV
                     VTTPSALLALEKLQGEQPPALILTDRMMPVMDGVEFCERVRANPQWRDVPIIMMSAAD
                     EPKERNQGLWNAYLHKPLDMDDLLHAVERIIGR"
     gene            complement(8575..>9314)
                     /locus_tag="VKZ48_03620"
     CDS             complement(8575..>9314)
                     /locus_tag="VKZ48_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HLU76978.1"
                     /translation="LGLLPHLQELRSKTGRPHWILVDETHHLLPTDWESAASMLGDGL
                     HSMIYITVHPDWVAPDVLSGVDIVAALGEEPYGTLENFARAAEIELPAARPLELEAGH
                     ALLWFCSTDEAPFVLEIEPSHSERRRHRRKYAEGELPPDRSFYFRGPGQKLNLRAQNL
                     MLFTQLAEGIDDETWLHHLRQGDYSRWMEDCVKDDELAEQVRAIEADEGLSPTDSRSR
                     VREAVEEHYTLPATNQPSSAAVPTRRT"
BASE COUNT         1669 a         3130 c         2877 g         1638 t
ORIGIN      
        1 ccgcgcccgc cacgccgatg atgatcgact gaccccagcc gcgatggcag cactccagcg
       61 cctggcgcat gagttcgacg ctgcccacgc attcgaagct gtagtcggca ccgcccttgg
      121 tgagttcgat gatgtagggc accagatcgc cctggatttc cttcgggttg atgaagtgcg
      181 tcatcccgaa cttgcgcgcc agcgcttcgc gctcggggtt gatgtccacg cccacgatca
      241 tgtcggcgcc caccatgcgc gcaccctgga tcacgttgag gccgatgccc cccaggccga
      301 acaccaccac gttggcaccg ggctccacct tggccgtatg gatcacggcg ccgatgccgg
      361 tcgtgacccc gcaaccgatg taacaaacct tgtcgaaggg cgcgtcctca cgaatttttg
      421 ccagcgcgat ctccggcatg acaccctgcc cctgcgtggc gcgaatggcc gtacagaggt
      481 tggtcttctg cgacaggcag gatttgcatt gacggcattc gggcgtatac agcggaatga
      541 cgtgatcgcc cttcttcaat gacgtgacac cagaccccac atcgacgacg atgcccgcgc
      601 cttcgtggcc gaagatcgcc gggaacaggc cttccgggtc ggcaccggag cgggtgaatt
      661 cgtccgtgtg gcaaatgccg gtcgccttga tttcgaccag cacttcgcct tccttcggac
      721 cgtcaagttg taccgtttcg accaccagcg gctcacccgg ggcatagcac actgcagcgc
      781 gtacatccat ggcgtctcct tccacaaata ccgagaatag ggttactgtt tacaagccta
      841 tgcggcgtgc gcataggaag caagggcgcg ccaaggcacg gcttttgcac cttgctcgac
      901 tcatccttat gtgcgccggt gcaaccgtgc tcagcgcggt ctgttctttc ggcgctcgtg
      961 cgatgactac ggaaaccgaa attcccgcct cggcctatgc cgaacaacgc gcccgcctgc
     1021 tgatcgatat cgcaggcatg gccgcctaca gcgcgagcta cacgggccgc agcagcatca
     1081 cgccgcgcgt gatgaaagcg atcgagcgcg tgccgcgcca tgcgttcgtg ccggaatcgc
     1141 aacgtgcaca tgcctacgag aaccgccccc ttcccatcgg cgaaggtcag acgatttcgc
     1201 agccctacat cgttgcgttg atgaccgatc tgctcgaact cgagccggac gatgtggtgc
     1261 tggaagtcgg cacgggctca ggctatcagg ccgcggtgct ggccgaactc gcggcgcagg
     1321 tctatacgat cgaagtgatc gaatccatcg gccgacgcgg cgccgaagtc ttgcgcgaac
     1381 tgggctacac caacgtccac acccgcatcg gcgacggcta tgccggctgg ccggagcacg
     1441 cgcctttcga cgcgatcatc gtcaccgccg ccgctcggca cgtgccgcag cccctgatcg
     1501 accagctcaa acccggcggc agactggtga ttccagtcgg gccggaaggc gggatgcagc
     1561 agttgttatt gttgcgcaag cagcgcgacg gctcggtcac ccagacagac accatccccg
     1621 tgcagtttgt tccactgaca cgctcgccct gatcgcgcgc gctgcgcgct atgattaggc
     1681 cttttttcgc gcgggcctct gtcgcgcgct tcgcatcgtg tccgcttccg ctacgcattc
     1741 ccccgatccc tcgttgctca gccaatgcga cgccgcctcc gcctgggcgc tcgtggacgg
     1801 cgccgcaccg gacagtgacg cgcaaggcgt ctgggtctgc accggtctgc tcgaacgcat
     1861 cgaagcgcac gtacccgatg cggtgtccga cgcctgcgcg cggatcgaag cggccggccg
     1921 cacgcatcct gtcgtcgcgc tgctggatta cgaactgggc tactggctgg aaccgcgcgc
     1981 cgcgctgcgt ccgccggagg tcgtgcgcgc gccgttgacc gcgctggtat tcgagcatgc
     2041 gacgtggctg ccgcacagcg cgttcgatga ggaactggcg cgtgctgtcg cggccctgcc
     2101 cgaagaagcg cgtcactgcg gcatcgcgga tctcacgcgc agctgcgacc gcgagagcta
     2161 tcgcgcggca atcgaacgta tcctgcagta tatccacgaa ggcgactgct accagatcaa
     2221 ctacacgtgg ccgatgcact tccggtgtta cggctcgccg ctcgcactct atctcgcact
     2281 gcgcaagcgc cagcccgtga cgcacggcgc gctggtccaa ctgccggacc gcacggtcta
     2341 ttctctttcc cccgaactct ttctggaacg ccacggcacg cgcctgcatt cgcggccgat
     2401 gaaaggcacg gcgccgcgcg gcaccacacc ggaagaagat cagcgcaacg ccgacgcact
     2461 gcaggcgtcc gaaaaagacc gcgccgagaa cgtgatgatc gtcgatctca tccgcaacga
     2521 tctcgggcgc gtcgcctcgc cgggttcggt gcgcgtggcc tcgctgttcg acatcgaacg
     2581 ctatcccacc gtgctgcaga tggtgtcttc ggtggtcgcc gatgcaccgg atgcctcgct
     2641 ccacgagatc cttcatgcgc tctttccctg cggctccatc accggcgcgc cgaaaatccg
     2701 cgccatgcaa atcgcgcaag aactggagcg cgggccgcgt cagctttata cgggcagcat
     2761 cggtttgatc cgtcccggcg gcgatttcag tttcagcgtg gtgatccgta cgctcgaagt
     2821 cgccgccgac ggcacgggcc gcctgggcat cggcagcggc atcgtggccg attccgatcc
     2881 cgaccgcgaa tacgacgaat gcctcgccaa ggcgcgcttc gccaccgatc tgccggcgga
     2941 cttcgaactc atcgaaacct tgcggctgga gccgcaggca gacgatccct atccgctgct
     3001 gcctcggcac ctggagcgct tgagcgcatc cgcgcgccac ttcggcttcg cttacgacga
     3061 ggggcgcgtt cgcgcggcgc tcgatgcgac gcgacgccag gcgggcgaag cgccgcatcg
     3121 cgtgcgtctg acgctggcga aatcgggcga gatcacggtg cgcagcgtac ccttcgcggc
     3181 cgctgccggg acgccgggcg tcgtgtatgc cgaagcggcc gtgcataccg ccaatccgtt
     3241 cctgcaccac aagaccacgg tacgcgggca atacgacgcg gcgctcaagg cgatcgaagc
     3301 gctccccgga tgtttcgatg cactgttctt caacgagcgc ggcgagttga ccgaaggcgc
     3361 aagatcgaac gtttatctcg tgcgggacgg caagtggtac acgccgccgc tgtcctccgg
     3421 cgtgctgaac ggcgtgatgc gccgggtgct gatggagacg cacacgccgc gcatcgaaga
     3481 gcgcgtgttg cgacgcgagg atgtggaagc cgccgaggag atccatctgt ccaacgccct
     3541 gcgcggcctg ttcagggtgc gcctgatgag ccggggctga acccagccgg gcgcgttact
     3601 tcaggctgct tttgtcgcgc cattcctcgc gcaactggct tgaattgcgg aactcgggat
     3661 ggatatggct ttccggcgtg ggcggcacat tgacgcgggc tgccggcgca gcgggaggcg
     3721 gaggctccgg cggctgcggt gcgacccgat ccaggcgctg gagccggtcg cgcaggcggc
     3781 gcagatcgtc cgccgagcag cggccgtaga gatagcacga gtcatagtag tagggatgcg
     3841 cgccggccca tacaagcatg cggacggcgc cggagtccgc atgcgcgacc gcggtggaaa
     3901 agccgcagag caacgccgct gcgccgagca ggatcaggcg gcgccgggtg cggggccgga
     3961 gcgcattcat ttccgccgcc gccccgggtg ccggtcagaa atcttcttcg agcgcctcat
     4021 cgggaacggg ctccggctgc tcctggcgga ccaggcggca agccagcaac tggtcggcaa
     4081 ggctcggtat gtggggcacg gtttcgcccg gctgcaggcg acgcgacaaa tgcaggaagc
     4141 cggtaccgtt gctgcgcagc gtgttgatgg attccattcg tccactccct ccattcgacg
     4201 caggtgcggg attgccggcc gaatgccggg cgcgtttcct ccaccccgcc ctacgccgtc
     4261 caactaagca gagcccatgc ctgatgacgc tctcgggtga gtaaagccgc gcgtgaacgc
     4321 gcagcagcgc acattccgcc ccagccgcga tgaacgatgc gtgatggatg ccgccgctcc
     4381 gcgcggcacg gggcgagggg cggcgggcac cgcgccctcc ccgctcgcaa tacggcgagc
     4441 gacatcgcga cgcagggagg ctaaaaatga ccgtcccgcg cagaaacgac gaagggcccc
     4501 gcagggccct tcgtcatcga acgcacaaag cctcgggctt taaccgttgg cgcggcggcg
     4561 gtactcgcct gtgcggctgt cgatctcgat cagatcgccg gtttcgcaga acagcggcac
     4621 cgcgatctcg aaaccggtgg ccagtttggc cggtttgagc accttgccgg aggtatcgcc
     4681 gcgcactgca ggttcggtgt aatcgatctt gcgcaccacc gtctgcggca attccaccga
     4741 aatggccttg tcgttgtaga acaccacctc gcactccatg ccgtcttcga ggtagttgag
     4801 cgcgtcgccc atgttgtcct tctcgacttc gtactggttg tattcgctgt ccatgaacac
     4861 gtacatggga tcggcgaagt acgagtaggt cacctccttg cggtcgagca cgacgacatc
     4921 gaacttctcg tcggcgcgat aaacattctc ggcggccgcg ccggatagga ggttcttgaa
     4981 cttcattttc acgaccgagg cgttgcggcc ggatttgctg aactcggcct tctgcaccac
     5041 catggggtcg ttcccgagca tgacgacgtt gcctacacgt aattcctgag cgattttcat
     5101 agctgtctaa gtctggcccg accttgcctc ggcgcccttg cccaggccgt cccgcgggtt
     5161 cctgttttca aaaagccgct tattttactt gccgttgcac gaacttgact agttcgctcc
     5221 ccagctccgg cccggattcg cgcaattgtg cccagcgcac cgcatgcgcc cgggcctgat
     5281 cctgatgcgc aagccattgc cgccacgccg cgccgactgt cggcgcgttc ggcacggcgt
     5341 tccatgccgc gtgcagcgcg cgctgggcag cggccgcttc tgcgggcagt tccgcgcaat
     5401 agcgatccag gaaggccgca agtttggcct ggtgcgcccc ctcctcctgc gggtaaatgt
     5461 gccagaccag cggtcgcgcg gcccattggg cgcgcacgaa ggagtcctcg ccgcgcacga
     5521 aattgaggtc gcaggcccat agcagccggt cgtagcccgc ctgatcgctg aagggcaaca
     5581 catgcaggcg gagcgcatcg cgaatcacgc attcgcccgg acgcaacgca ccgcaaccga
     5641 aatacgccgc aatgggtgca accaccggcc cggtcggcac cagcgccgtg acgggaaagg
     5701 agcctgtgcg ccacgcgtcg agcaactcgc ccaccggcgc gtgcccgtag caaaacaggg
     5761 acacccggcg ctcatccggc gcaggcaacg gcaggccctg cgtctgccag taggtggcca
     5821 gcgcctgcgc gtcctgctgg aaagcgcgtc ttgccgctat cagtccgcgc tcgcgcaaca
     5881 atccgcccgt gccctcctcg aaaccgggga aaaagaaata tttgtgcaag ggcaggcggg
     5941 gatgcaccga cggcccgcga tggcagccgc gcacccagtc ttcggcactg aggtattcca
     6001 gattcagcca gaccggctgc ggccgccgga ctgccatggc ggcaagaaac gtctcaggca
     6061 gccgacaccc gaacgcctcg atcactacga cacccggctc gacctgcgga aagggttcgc
     6121 tccaggcgcg aatctccacg ccctccaccg tctgcactgc ggcattcgcg tcgacagccg
     6181 gcgccagttg ggacagcgtc cgcaaatcgt ccaaccacag ccgcacggga atgccgtgat
     6241 cgcggcgcag cgtgcgcgcc agccgccatg acacgccggc atcgccatag ttatcgatga
     6301 cggaacagaa gagatcccac atgagttggc cgagcgctca gcgcagaaag tcgagagtaa
     6361 aaccacgcgt ggagtcggag tgtggccgaa gaaccgcgcc ggctgcaatt cgcagcctcg
     6421 cggcgatccg cggattcatg gtcgcgccat ccaagaaggc gggcctgggg cgcctcgaag
     6481 cgttcgcgcg cctgtcgcct gtgcgccccg ctctctccac tctcggctct ccactctcgg
     6541 ctttcgactt tttccattgg catgggtgtt gcatcccgag ctaaaaattc cctgtcgtca
     6601 ttccgtcgat gatgcgtgcg cttgcggccg tttcatcgac gcccgcaaac gaacaacgca
     6661 gaagagcctt cgcgcttccc gtttggcgca tgccgaccgg aagcgcccac tcgaacagga
     6721 tcgctccatg agtctacaca gcgacgcgct tccgcagcgc atctccaccg gcattgccgg
     6781 cctggatagc gtcctgggtg acggcctgat gcagcacggc atgtatctgc tcgaggggcc
     6841 acccggcagc ggcaagacga ttctttcggc acagatctgt tttcatcatg cacacgctca
     6901 acgcaagacg ctttacgtga cgctcatcgc cgaatcgcac ggcaagctgc tcaacaatct
     6961 gcgcgccttc gacttctacg atcccgcgct ggtacccgag cgcttcatcc tgatgagcgg
     7021 ctatcaggaa ctcaagacct cgggcctgcc gggtctgctg caactcgtcg cgcgcgcgct
     7081 ggcggaacat cgccccagcg tgctggtgat cgacggctac cgcagtctcc gtatgctcgc
     7141 gccggacgcc gccacgaccg ccgagttcat ctacgaactg aacacgctca tcaccaccac
     7201 caaatcgctt tgcctgctgc tcgcgccgcg cgacgacagc ggcgcatggg ccgagaagac
     7261 gctgatggac ggcgtcatcg aacttcaccg tttttcggtc ggcatgcgca gcgtacgcga
     7321 agtggaagta cacaaactgc gttcctcggc gccgatcgaa gggcgtcatg ccttcaaaat
     7381 ctgctctgcc ggcatccggg tgtttccccg cctcgaagca agcgcgccgt ttcgcaacgc
     7441 cgcaccggac cgcgaggcga cccgctgcac cttcggtatc gaaggcatgg acgacatgct
     7501 ccacggcggc gtattgcgcg acagcgccac ctgcctgctc ggcccgcccg gatcggggaa
     7561 gacgatcttc ggcctgcatt ttctccatca aggcttgcag gccggcgaca ccgggcttca
     7621 cttcggcttc tacgaatcgc ccgcgcagtt actcgacaag gccgcaaacc tcggcctgga
     7681 tttgaacgaa gcgcatcgca acggacagtt cgacatcctc tggcagccgg cgctggaatg
     7741 ccggcccgac gaactcgcca tgcagttgct ggaaaacgtc tggcagcgca aggcgcggcg
     7801 cgtagtcatc gacggcctcg aaggttttat gcaaagcgcg gtgagaccgg aacggatcgg
     7861 tcgctttttc aatgcgctgg tcggcgagtt acggcgtcag caggtcgcct gcctgtacac
     7921 cgaggaggta ccgctgttcg cccgcgaagt cagccacggc acgctggcca catcggcgat
     7981 cgtcgacaac cagatcctgc ttcgcttcgt gcgacacgag gccggcatgc gacgcaccgt
     8041 tgccgtgctg aagcagcgcg aaagcggcca tgaccacggc gtgcgcgaat tctggatcga
     8101 gcgcgggggc atacgcgttg cgtccgacag cgacagcgcg cacgccttcc tcgcggagcg
     8161 gccatgatcg cgcagacgga cacggacacc ctcacttcga tgaaaagcgc gactaaaagc
     8221 atcctggtcg tcgacgatga gttcgatgtg cgcgcaatcg tcgagttgat cctcgggatg
     8281 cagaattacg atgtggtgac gacgcccagc gccctgctcg cgctggagaa gctgcaaggc
     8341 gaacaacctc cggcgctgat cctgaccgat cgcatgatgc cggtgatgga cggtgtggaa
     8401 ttctgcgagc gcgtgcgcgc caatccacag tggcgggatg tgccgatcat catgatgagc
     8461 gctgccgacg aacccaagga acgcaatcag ggtctttgga acgcctatct gcacaagccg
     8521 ctcgatatgg acgacctgct gcacgcagtc gaacgaatca tcggccgata gccgtcaggt
     8581 gcgtcgtgta ggcactgccg cggaactggg ctgattggtg gccggcagag tgtaatgctc
     8641 ctccaccgct tcgcgcacgc gagagcggct gtcggtcggc gacaggccct cgtccgcttc
     8701 gatggcgcgc acctgctccg ccagttcatc gtccttcacg cagtcctcca tccagcgcga
     8761 ataatcgccc tgccgcaggt ggtgcaacca ggtttcgtcg tcgatgcctt cggccagttg
     8821 cgtgaatagc atgaggttct gtgcgcgcag attgagtttc tgccccggcc ctcggaaata
     8881 gaagctgcga tcgggcggca actcgccttc ggcatacttg cgccgatggc gacggcgttc
     8941 gctatgactc ggttcgatct cgagcacgaa gggcgcttcg tccgtcgagc agaaccacag
     9001 cagtgcatgt ccggcttcca gttccagcgg ccgggccgcc ggcagttcga tttccgcggc
     9061 gcgggcaaag ttctccagcg tgccgtaagg ctcctccccc aacgcggcga cgatatccac
     9121 gccgctcagc acatcgggcg ccacccaatc cggatggacg gtgatgtaga tcatcgaatg
     9181 cagtccgtcg cccagcatgg aggcggccga ctcccaatcg gtcggcagca gatgatgcgt
     9241 ttcatcgacc agaatccagt gcggcctgcc ggtcttggaa cgcagttcct gcaaatgcgg
     9301 caacagcccc agga
//
LOCUS       DATGOU010000065         9270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_1497930, whole genome shotgun sequence.
ACCESSION   DATGOU010000065 DATGOU010000000
VERSION     DATGOU010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9270
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497930"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..771)
                     /locus_tag="VKZ48_03625"
     CDS             complement(40..771)
                     /locus_tag="VKZ48_03625"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HLU76979.1"
                     /translation="MASLLFYQNPVPLSREAHKNLRIKMNAGDFGFAAHTNSVPLAAA
                     EFYDAQRDYPIVFAGQAGEVAPVALLGLRQSENLFIGKGKQWDSDYVPVFARCYPFVL
                     ARGKGPDYLVCVDENSPAIVKSGGESLFNEDGQETDYLRRNIDLLSEFQSHMRRTREF
                     TQRLKEAGLLKDITLQVVPEGGEPLSLQGVQVVDEAKLMALDDAKVTELFRLGELGWI
                     YAHLASLRNVQRLSKRLSRRLVKAA"
     gene            1060..2106
                     /locus_tag="VKZ48_03630"
     CDS             1060..2106
                     /locus_tag="VKZ48_03630"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HLU76980.1"
                     /translation="MDTLTHALSGALSARATAPRTGALPLRRRVGIGFVVAALPDLDV
                     VGTWVSPLFYLEHHRGVTHSLLMLPIWTVLLALLLSRLWRGGPGWRAYAGVVALALFV
                     HIVGDWLTSFGTMVFAPLSDARFALGTTFIIDLWFSGIILAGLLASLLWRRTRVPAIV
                     GTLVLAGYVGLQAVARDHALEFAEGYAQAQGMTDASVTASPRPVSPFNWTLIVVDGET
                     LHLSHVNVLRDDLLTLTPEAGFLRRLDAPYQPLESATWQTVSRFGEDGVRDLAREAWS
                     QPELGFFRWFAQHPVLYRVDNGRDSVCAWFQDLRFLTPGRDAMPFRYGLCRAGNSAWR
                     RFQLLEDGAALPLN"
     gene            2440..2802
                     /locus_tag="VKZ48_03635"
     CDS             2440..2802
                     /locus_tag="VKZ48_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76981.1"
                     /translation="MSADIKDSAAYNDELERLHAQEMKALVEAIETEFADLTGPQIQI
                     VHGCAMMICGLTQLDTWEDVPAAQLQAERSLSDGFLKRLDEAVPEALRPRVRQAVKMH
                     AERYARLAVEQRLGETLH"
     gene            2811..3251
                     /locus_tag="VKZ48_03640"
     CDS             2811..3251
                     /locus_tag="VKZ48_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76982.1"
                     /translation="MRDGAVTRFLNTLETATPSGKRKQLLDKLESLIGERATEFEHLA
                     TPELAFVMGSALWKFDLMGEATWEGLSAEQFEALRPAITGAMTAGIERWPRHLNEAEY
                     LLFRYLTERYGEEYITEMSWDVSAPTRIGSSATLGKKSRNDEPS"
     gene            complement(3311..4075)
                     /locus_tag="VKZ48_03645"
     CDS             complement(3311..4075)
                     /locus_tag="VKZ48_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU76983.1"
                     /translation="MTASSMPLLPWPVQVGPYGFTSLIDPPSRFTRPRWLSETDYNHQ
                     IIRVRHGLSPQQIAFNWWLRVVRAMHYSAGLDDGCPEESFTQAYAAGLIAFIRANPET
                     WVWFNQLVEAQTSPGVKYARYAAGRAGGAERLAPPRQMKVGKSVYQLESMPLDLSERL
                     NCWGDCNLSTKVVRLSAELYGTQLAVIFWHELVHAMHREDELDDGHSRARFARSQAAR
                     TIQFMVDNPQAWRWFLCLTSQAENDGRAQQRLRHVA"
     gene            complement(4280..4918)
                     /locus_tag="VKZ48_03650"
     CDS             complement(4280..4918)
                     /locus_tag="VKZ48_03650"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HLU76984.1"
                     /translation="MKRVAACGLLCALLLYGAAAGAVTLEDFFEAAETGDVAALANLL
                     DRGMPVDAADRQGYSALMYAARAGQLEAVRLLLERGAKVNARNALGETALMLAAFNGH
                     QVTMAFLLRLGATVDAGEGQWSALHYAAHQNRVEVLRLLLERGADPNSRAVNGITPLH
                     MAAREGAVAAIGLLLRAGANPRLQTVSGDTAQAWAEAAGHQEAARLLAGSRQ"
     gene            complement(4915..5700)
                     /locus_tag="VKZ48_03655"
     CDS             complement(4915..5700)
                     /locus_tag="VKZ48_03655"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HLU76985.1"
                     /translation="MFIDSHCHLDFEDFRDRMPQVLDAMSRADVTHALCISVTLEDFP
                     AVRALAEAHDNLYASVGVHPDYPDAPPVTAEQLVALADHDRVIGIGETGLDYYRLTGD
                     LEWQRERFRAHIRAAREAGKPLIVHTRSAAEDTLRLLREERAEEAGGVLHCFTESQEV
                     ADAALEMGFYISFSGIVTFKNAVALKEVARRVPLDRMLIETDSPFLAPVPHRGKTNEP
                     AYVRHVAEEIARLRDEPLETIAAATTANFFRLFAAANPNVAPA"
     gene            complement(5741..6730)
                     /gene="holB"
                     /locus_tag="VKZ48_03660"
     CDS             complement(5741..6730)
                     /gene="holB"
                     /locus_tag="VKZ48_03660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011885039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HLU76986.1"
                     /translation="MSEQIFPWQIAAFNGLAARGEQLPHALLIQGRAGIGKTVFARAF
                     AAWLLCEGARSETAACGECEPCRWFEQGNHPDFRQIEPEFLSPEMEGVERSKEPSRQI
                     KIEQVRELQRFLAVGTHRAGRRVAIVRPAEAMNVATANALLKSLEEPPPDTLFLLVAS
                     QPARLLPTVRSRCQAVHLPAPDAETAAQWLAAQGFDEARAMLAHCANAPLAAIEAADQ
                     RPLRDRLLTQLARGESDPLVLADACAGAEPVQVIDWLQKWIYDLSAVQFAGEPRYHPQ
                     ARAALQKLVASLAPRRLLAYQRELAHRRAVAQHPLNPRLFLEELFMRYRALRD"
     gene            complement(6767..7396)
                     /gene="tmk"
                     /locus_tag="VKZ48_03665"
     CDS             complement(6767..7396)
                     /gene="tmk"
                     /locus_tag="VKZ48_03665"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006160632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HLU76987.1"
                     /translation="MRGKFITLEGIDGAGKSSHVEWIANRIRAAGKQVVVTREPGGTP
                     LGEKLRTLLLEESMDLRTEALLAFAARQEHLVSLIRPTLDAGTWVLSDRFTDATYAYQ
                     GAGRGLSLDRIRQLEQWVHDGLQPDLTCYFDVPSEVGQARRRAAGRDLDRFEREAGEF
                     FDRVRAGYLARVEQSGGRMRVIDGTRPLVEVRKELSEILASIGINEKKT"
     gene            complement(7407..8408)
                     /gene="mltG"
                     /locus_tag="VKZ48_03670"
     CDS             complement(7407..8408)
                     /gene="mltG"
                     /locus_tag="VKZ48_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HLU76988.1"
                     /translation="MWKLLKAAVLLIVLAGVVFGGWMLWFASAPIKQPRPDVEFAIQP
                     GSGLRAAVRQMQAAGLEFSGPSFVLMARALGRAGNIKAGYYALAEGATPLDLLDKITR
                     GDFVQRSILLVEGWTFAQARAALDAHPDVRHDTLGLSDEEVMARIGRPGVLPEGRFFP
                     DTYVFAKGTSDVDILKRAYDSLARHLAQAWEERDADLPLKTEEEALILASIVEKETGR
                     AEDRATIAAVFHNRLRIGMLLQTDPTVIYGMGERFDGNLRRNDLRTDTPYNTYTRRGL
                     PPTPIALPSRAAIDAVMHPADTDALYFVARGDGSSHFSRTLDEHNRAVNRYQRGGRQ"
     gene            8492..>9270
                     /locus_tag="VKZ48_03675"
     CDS             8492..>9270
                     /locus_tag="VKZ48_03675"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HLU76989.1"
                     /translation="MSLSDLPPFARARFAGDLVVDFGDAATELRATAVERVVAPLTQL
                     TVLRFSGADAENFLQGQLTCDVAALQPGRATPGGYCTAQGRLLATFLLWREAEGYAML
                     LARDIAEAVQRRLQMYVLRAKVTIENAGAQWIALGLSGPQAAEALADPGFAPAASDYA
                     FGKAEGYALLTLPRGRYIALAVTDALEALWPGLTSSFKPVGTPCWQWLDIAHGLPWIT
                     AATQDKFVPQMVNLELIGGVNFQKGCYPGQEVVARTQYRART"
BASE COUNT         1601 a         3054 c         2907 g         1708 t
ORIGIN      
        1 cggtattgaa aaccgggcag cgtcaacgaa ccgcttagtt caggccgctt tcaccaaacg
       61 ccggctcagc cgcttcgaca gtcgttgcac gttgcgcagc gaagccagat gcgcgtagat
      121 ccagcccagt tcaccgaggc ggaacaactc cgtgactttg gcatcgtcga gcgccatgag
      181 cttcgcttca tccaccactt gcacgccttg cagcgacaag ggttcgccgc cttccgggac
      241 gacttgcagc gtgatgtcct tcagcaggcc ggcttccttg aggcgctggg tgaactcccg
      301 ggtgcgccgc atatggctct ggaattcgct taacagatcg atgttgcgac gcagataatc
      361 ggtttcctgg ccgtcttcat tgaacagcga ctcgccgccg ctcttgacga tggcaggcga
      421 gttttcgtcc acgcagacca gatagtcggg gcctttgccc cgcgcgagca cgaagggata
      481 gcagcgtgcg aataccggga catagtcact gtcccactgc ttgcccttac cgatgaagag
      541 attttcgctt tgccgcaaac cgagcagcgc tactggcgca acttccccgg cctgaccggc
      601 gaacacgatg gggtaatccc gttgggcgtc gtagaactcg gccgccgcga ggggtacgga
      661 gttagtgtgg gctgcgaagc cgaagtcgcc cgcgttcatt ttgatgcgca ggttcttgtg
      721 cgcctctcgg ctcaaaggaa cagggttctg atagaacaac aggctcgcca cagcttctcc
      781 ctcccggaca tcttgtattt atggccacac tggcagcgtg gactcgcgca ttcggacaat
      841 cgggcaattt cccgaatcgg cggttggcgg agtgtaccgg cgtcgctttt tgacgacaat
      901 taaaaataac aatgtcgggt gtaggtattt cgacaccttc ccttgatttg catgggctat
      961 ttgtgcggcc acgctgtcgc gggccgactg aggcggcaga gcaatcgatc tttgctacag
     1021 tcctccgcct gcatcgcccg cactgaaggc tccccgtcca tggacaccct cacccacgcc
     1081 ctctccggcg cgctgagcgc ccgcgcgacc gcaccgcgca ccggtgcgct gccgttgcgc
     1141 cgacgtgtcg gcatcggctt cgtggtcgca gcgctgccgg acctggatgt ggtcggcacc
     1201 tgggtgtcgc cgctgttcta tctggaacat caccgcggcg tgacccattc actgctcatg
     1261 ttgccgattt ggacggtgct gctggccctg ctgctcagcc ggctctggcg cggcggtccg
     1321 ggttggcgcg cctacgccgg cgtcgtcgcg ctggcgctgt tcgtacatat tgtgggggac
     1381 tggctgacgt ccttcggcac aatggtgttt gccccgctgt cggacgcacg cttcgcgctc
     1441 ggcaccacgt tcatcatcga tctgtggttc agcgggatca ttctggccgg gctgctcgct
     1501 tcgctgctct ggcggcgcac acgcgtgccc gccatcgtcg gcacccttgt gcttgccggt
     1561 tacgtcggcc tgcaggccgt ggcgcgcgat catgctctcg agttcgccga gggttatgca
     1621 caggcacagg gcatgacgga cgcatccgtc accgcctcgc cccgaccggt ctcgcccttc
     1681 aattggacct tgatcgtggt cgacggcgag acgctgcatc tgagccatgt gaacgtcctg
     1741 cgggatgacc tgctgacgct tacgccggaa gccggtttcc tgcgccgcct ggatgcgccc
     1801 tatcagccgc tcgagtcggc gacctggcag accgtctccc ggttcggaga agatggtgtc
     1861 cgcgacctgg cgcgcgaggc gtggtcgcaa cccgaactcg gcttcttccg ctggttcgca
     1921 cagcatccgg tgctctatcg cgtcgacaac ggccgcgaca gcgtctgcgc ctggtttcag
     1981 gatctgcgct tccttacgcc cggacgtgac gcgatgccgt ttcgctacgg cctttgccgc
     2041 gccggcaaca gcgcctggcg gcggtttcag ttgctcgaag acggcgccgc actgccgctg
     2101 aattgatgcg gctacgctcc cggcgacaat agcggcatcg cgactttatc gatcgcacgg
     2161 aatccgcaca tcgcattcgc cagcggctgc gctttcgtgg gctgcggcac gaccaccgcc
     2221 atcgtcatcc gcaagcgttt tggcatccga ttccgcttgc ccgtcgcgct gccgcatccg
     2281 gacactgctc cgctcctcgg cacgtgtgaa aaaccacaca tttgcaatcc gtgcccgccc
     2341 ttgattgacg ccgaccgtgg ctaatgaaac ggcgcgcgcg gccgaagaag catgtaatgg
     2401 cgccgaagtc gtttcaacct cattagcgag cgcccagaca tgtcagccga tatcaaagat
     2461 tcagccgcgt acaacgacga actcgagcgc ctccacgcgc aagagatgaa agcgttggtg
     2521 gaagcgatcg aaacggaatt tgccgacctc accggtccgc agattcagat cgtccatggt
     2581 tgcgccatga tgatttgcgg cctgacccaa ctcgacacct gggaagacgt gccggcagcg
     2641 cagttgcaag ccgagcggtc gctgtcggac ggcttcctca aacggctcga cgaagccgtg
     2701 cccgaagcgc tgcgaccgcg cgtccgtcaa gcggtcaaga tgcacgccga gcgctacgcg
     2761 cgcctggcgg tggaacagcg cctgggcgaa accttgcatt aggagaagcc gtgcgggatg
     2821 gcgccgtcac ccgatttctg aacacgctgg aaaccgcgac cccatccggc aagcgcaagc
     2881 agttgctgga caagctggaa agcctgatcg gcgaacgcgc cacggagttc gaacaccttg
     2941 ccacgccgga actggcgttc gtcatgggct cggcgctgtg gaagttcgat ctgatgggcg
     3001 aagccacctg ggaaggcctg agcgccgagc agttcgaggc gttgcgcccc gccattaccg
     3061 gtgccatgac ggccggcatc gagcgctggc cgcgtcatct caacgaagca gaatacctgc
     3121 tgttccgtta tctcaccgag cgttacggcg aggaatacat caccgagatg agctgggatg
     3181 tgtccgcgcc tacgcgtatc ggcagcagcg cgacgctagg caagaaaagc cgcaacgacg
     3241 aaccgtcctg aaccgcccct gccgggattg ccccggcagc gtttacatgg agaacgacgc
     3301 tcggccgcga tcaggccacg tgccgcaggc gttgctgcgc acggccgtcg ttttcggctt
     3361 gggaggtcag gcagaggaac cagcgccacg cttgcgggtt gtcgaccatg aactggatcg
     3421 tgcgtgcggc ctggctgcga gcgaagcggg cgcggctgtg gccgtcgtcg agttcgtcct
     3481 cacggtgcat ggcatgcacc agttcgtgcc agaagatcac cgcgagctgc gtcccgtaga
     3541 gctccgctga cagccgcacc accttggtcg acaggttgca gtcgccccag cagttgaggc
     3601 gctcggacag atcgagcggc atgctttcca actgatagac gctcttgccc accttcattt
     3661 gcctcggcgg cgccaggcgt tccgcaccgc ctgcccggcc ggcagcatag cgcgcgtatt
     3721 tcacgcccgg cgaggtttgc gcctccacca actggttgaa ccagacccac gtctccggat
     3781 tggcgcggat gaatgcgatc aatccggccg cgtaggcctg ggtgaaggat tcttcggggc
     3841 aaccgtcgtc gaggccggcc gaatagtgca tggcgcgcac tacgcgcaac caccagttga
     3901 atgcgatctg ctggggcgaa agcccgtggc gcacgcgaat gatctggtgg ttgtaatcgg
     3961 tttcggataa ccagcgcggg cgggtgaaac gcgacggtgg atcgattaat gaagtgaagc
     4021 catatggccc gacttgcacg ggccagggca gcagcggcat tgaactggca gtcatgattt
     4081 cggtacccgg taagttgatg cccggtgtgc gggcggcgcc cgtgaccggg cggagaatgg
     4141 cccttaggat accgcgtcaa aaccgctttg acagtcaaac gtccgtttga cttacatgat
     4201 gtgacacatc atgtatttac tttaatccac tgattaatat gtaattccgt taggcgggat
     4261 gcgagcccag gacaaagttt tactgcctcg acccggcaag cagcctcgca gcctcctggt
     4321 gcccggccgc ttcggcccag gcttgcgcgg tgtctccgga cacggtctgc aggcgcggat
     4381 tcgcaccggc gcgcaacagt aatccgatcg ctgcaactgc gccttcgcgt gccgccatat
     4441 gcagtggcgt gatgccattc actgctcgtg agttgggatc ggcgccgcgt tcgagcagca
     4501 aacgaagcac ctcgacgcgg ttctgatggg cggcgtagtg caacgcactc cactgccctt
     4561 ctccggcatc caccgtcgcg ccgagccgga gcagaaacgc catggtcacc tgatggccgt
     4621 tgaaggccgc aagcatgagc gcggtttcgc cgagcgcatt gcgtgcgttg accttcgcgc
     4681 cgcgttcgag gagcagccgc accgcttcca attgccccgc tcgcgccgcg tacatcagcg
     4741 cgctgtagcc ctgcctgtcg gcggcgtcca caggcatccc ccgatccagc agattcgcca
     4801 gcgcggcgac gtcgcccgtc tccgcggcct cgaagaaatc ctccagggtg accgcaccgg
     4861 ccgctgcgcc gtaaagcaga agcgcgcaca gcaggccgca tgcggcaacc cgtttcatgc
     4921 cggcgccaca ttcggattcg cggcggcgaa cagccggaag aagttggccg tcgtggccgc
     4981 tgcgatcgtc tccagcggct cgtcacgcag gcgcgcgatc tcttccgcga catggcggac
     5041 gtaggccggc tcgttggtct tgccgcgatg aggcaccggc gcaagaaacg gcgaatccgt
     5101 ttcgatgagc atgcggtcca gcggcacccg cctggccact tccttcaacg caaccgcgtt
     5161 cttgaatgtc acgatgccgg aaaaggagat gtagaaaccc atctccaggg cggcgtcggc
     5221 cacctcctgc gactcggtga agcaatgcag caccccgccc gcttcctccg cgcgctcttc
     5281 gcgcaggagc cgcagcgtgt cctcggcggc ggaacgggtg tggacgatca gcggcttgcc
     5341 cgcttcgcgt gcggcgcgga tgtgcgcgcg aaagcgctcg cgctgccatt ccagatcgcc
     5401 cgtcagccgg tagtagtcga gccccgtttc gccgatgccg atcaccctgt cgtgatctgc
     5461 cagcgccacg agctgttcgg ccgtgaccgg cggcgcgtcc ggatagtccg gatgcacgcc
     5521 caccgaggca tacagattgt cgtgcgcctc ggccagcgcc cgcacggcgg gaaaatcctc
     5581 caaggtgacg ctgatgcaca gcgcatgcgt cacgtcggca cgcgacatcg catccagcac
     5641 ctgcggcatg cggtcgcgga aatcctcgaa atcgaggtga cagtgtgaat cgatgaacat
     5701 cgcaaacagg gttggtgccc cgcagggcgg ttgaaaggat tcagtcgcgc agggcgcgat
     5761 agcgcatgaa caattcttcc aggaagaggc gcggattgag cgggtgctgt gcgaccgcgc
     5821 ggcggtgcgc aagttcgcgc tggtaggcga gcagccggcg cggcgcgagc gacgcaacga
     5881 gcttttgcag ggccgcgcgc gcctgcgggt gatagcgcgg ctcgccggcg aactggacgg
     5941 cgctcagatc gtagatccac ttctgcagcc aatcgatcac ctggaccggt tccgcgccgg
     6001 cgcaggcatc tgccagtacg agcggatcgc tctcgccccg cgcgagctgc gtcagcagtc
     6061 ggtcgcgcaa ggggcgctga tcggccgcct cgatcgctgc cagcggggcg ttcgcgcaat
     6121 gcgccagcat ggcgcgcgcc tcgtcgaatc cctgtgcggc cagccattgc gcggcggtct
     6181 cggcatccgg cgccggcaga tgcaccgcct ggcaacggct gcgcaccgtc ggcagcaggc
     6241 gcgccggctg actggcgacc agcagaaaca gcgtatccgg cggcggctcc tccaggcttt
     6301 tgagcaaggc gttggcggtg gcgacgttca tcgcttcggc cggccgcaca atggcgacgc
     6361 gccggccggc acggtgcgtc ccaaccgcga gaaagcgctg cagttcgcgc acctgctcga
     6421 ttttgatctg cctgctcggc tccttgctgc gctcgacgcc ctccatttcc gggctgagga
     6481 attcgggctc gatctggcgg aagtccgggt gattgccctg ctcgaaccag cgacagggtt
     6541 cgcattcgcc gcaggcggcg gtttcactgc gggcgccctc gcacagcagc catgcggcga
     6601 aggcccgtgc gaaaacggtc ttgccgatgc cggcgcgtcc ctggatcagc aacgcatgcg
     6661 gcagttgctc gccgcgcgcc gccaagccgt taaatgcggc aatttgccag ggaaatattt
     6721 gctcgctcat ttacttacgg aattcactta aaccggcttg ttcggttcaa gtttttttct
     6781 cgttgattcc gatacttgca agaatctctg aaagctcctt gcgaacttcg accagcggcc
     6841 gcgtcccgtc gatgacccgc atccgaccgc cgctctgctc gacacgtgcc agatacccgg
     6901 cgcgcacccg atcgaagaat tcgcccgctt cccgctcgaa gcgatccaga tcgcgaccgg
     6961 ccgcgcgccg gcgcgcctgc cccacttcgc tcggcacgtc gaaatagcag gtcaggtcgg
     7021 gttggagtcc gtcgtgcacc cactgctcca gttgccggat cctgtccagc gacagtccgc
     7081 gcccggcgcc ctgataggca taggtcgcat cggtaaagcg gtcggacagc acccaggtgc
     7141 cggcatcgag ggtcggccgg atcagggaga ccagatgctc ctggcgcgcc gcgaaggcca
     7201 gcagcgcttc ggtgcgcaga tccatggatt cttccagcag cagcgtccgc aacttttcgc
     7261 ccagcggcgt gccgcccggc tcgcgcgtca ccaccacctg cttgcccgcc gcacgaatgc
     7321 ggttggcgat ccattcgacg tggctgcttt tgccggcgcc gtcgatgcct tccagagtga
     7381 tgaatttgcc gcgcatgatc gcttttctat tgtcggccgc cgcgctgata gcggttgacg
     7441 gcgcggttgt gttcgtcgag ggtacgcgaa aaatggctcg aaccgtcacc ccgcgccaca
     7501 aaatacagcg catcggtatc ggccggatgc atcacggcat cgatggcggc ccgactcggc
     7561 agcgcaatgg gcgtcggcgg caggccgcgc cgcgtgtagg tgttgtacgg cgtatcggtg
     7621 cgcaggtcgt tgcggcgcag attgccgtcg aagcgctcgc ccatgccgta gatcaccgtg
     7681 gggtcggtct gcagcaacat gccgatgcgc agccggttat ggaagacggc ggcgatcgtc
     7741 gcccgatcct ccgcgcgccc tgtctccttc tccacgatcg aggcgagaat gagcgcctct
     7801 tcctccgttt tcagcggcag atccgcgtca cgctcttccc aggcctgcgc cagatggcga
     7861 gccagcgagt cgtaggcccg cttgaggata tcgacatcgc tcgtgccctt tgcgaacaca
     7921 taggtatcgg gaaagaagcg gccttcgggc agcacgcccg gccggccgat gcgcgccatg
     7981 acctcctcgt cggacaatcc gagcgtgtcg tgtcgcacgt ccggatgtgc gtcgagcgcc
     8041 gcacgcgcct gtgcgaaggt ccaaccctcc accaacagga tactgcgctg cacgaaatcg
     8101 ccgcgcgtga tcttgtccag cagatccagc ggcgtggcgc cttcggcaag cgcgtaataa
     8161 ccggccttga tattgcctgc gcgaccgagc gcgcgcgcca tcagcacaaa gctcggaccg
     8221 gaaaattcca gtccggccgc ctgcatctgc cgtaccgcgg cacgcagccc gctgcccggc
     8281 tgaatggcga attccacatc gggccggggc tgcttgatgg gcgcactggc gaaccacagc
     8341 atccagccgc cgaaaaccac gccggccagt acgatcagca gcaccgctgc tttcaacagc
     8401 ttccacatag caaagcgcgc ccattcgggc gcgggtcgga taaaaagccg cccgtataat
     8461 aaccgtcacc acgctcaacg cattcgcatc catgtccctt tctgatttgc cgcccttcgc
     8521 ccgggcgcgg tttgcgggcg atctggttgt cgatttcggc gatgccgcga cagaactgcg
     8581 cgccactgcc gtcgaacgcg tcgtcgcgcc cctcacgcaa ttgaccgtcc tgcgcttcag
     8641 cggtgccgat gccgagaact ttctgcaggg ccaactgacc tgcgacgtcg ccgcactcca
     8701 acccggccgg gcgacgcccg gcggctattg caccgcccag ggtcggctgc ttgcgacctt
     8761 tttgctgtgg cgggaagccg agggctacgc catgctgctt gcgcgcgata tcgccgaggc
     8821 cgtccagcgc cggctgcaga tgtatgtgct gcgcgccaag gtcacgatcg aaaatgccgg
     8881 cgcgcagtgg attgcactgg gtttgagcgg cccgcaggcc gccgaggcgc tcgcggatcc
     8941 gggctttgcg ccggcggcct ccgattacgc tttcggcaag gccgagggtt acgccttgct
     9001 gacgctgccg cggggccgat acatcgcact tgcggtaacc gatgcactcg aagcgttgtg
     9061 gcccggactc acaagttcgt tcaagcccgt tggcacgccc tgctggcaat ggctggacat
     9121 cgcgcacgga cttccctgga tcaccgctgc aacccaggac aaattcgtgc cgcaaatggt
     9181 gaatctcgaa ctgatcggcg gcgtgaactt ccagaagggc tgctatcctg gccaggaggt
     9241 cgtcgcacgt acgcagtacc gcgccaggac
//
LOCUS       DATGOU010000066        19101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_613038, whole genome shotgun sequence.
ACCESSION   DATGOU010000066 DATGOU010000000
VERSION     DATGOU010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19101
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613038"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="VKZ48_03680"
     CDS             <1..713
                     /locus_tag="VKZ48_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLU76990.1"
                     /translation="IVNEEIATFMQGRKETTPLPVELAVRVRFNPELNKSWFGAVIQV
                     INQVTMLSIILTGAALLREREHGTVEHLLAMPVTPFEIMTGKLWSMGLVVLLATGISL
                     VTVVQGALGVPIGGSMALFLCGAALNLFATTSLGIFLATIARSMPQFGLLLILVLLPL
                     QILSGGATPRESMPEIVQVVMLGAPTTHFVLLAQAILFRGAGFDIVWPQFVALALIGT
                     ALFVYSLTRFRRAIASMA"
     gene            767..1297
                     /locus_tag="VKZ48_03685"
     CDS             767..1297
                     /locus_tag="VKZ48_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HLU76991.1"
                     /translation="MKPLQLGLSLTLAAALTCAAPAVAAEPFAPYGTAKVDMHDMPVT
                     NVVFDVNYQDPKSLDILYHFIRNTWKETKGKVVVVSHGPELRAFAKENYEKYQAVVDQ
                     LAELAQEGVEFKMCNNALRAAGFQPEDMHGFVTVVPAGFPELAYWQSKGYQYINPLPL
                     PVGGVRYLDQPQLKKP"
     gene            1328..4195
                     /locus_tag="VKZ48_03690"
     CDS             1328..4195
                     /locus_tag="VKZ48_03690"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HLU76992.1"
                     /translation="MPEYAFDEVSVSGVNDRDTAAASAHTQSVEEIAQALRSDPACGL
                     TQREAQARLRHSGPNELAHEKPLPAWRKFLAQFGNVLVILLLVAAAISALLWAIEPDA
                     ALPYEAIAILVIVLLNALMGYIQQARAERAVAALKQMSAAHATVIRDGERRRIPAAEL
                     VPGDVILVEEGDTVPADARVVESTALQTAEAALTGESLPVAKDTEPVAAQAPLGDRAG
                     MIYSGTAVTYGRGRAIVTATGMRTEMGRIAGMLEAAPAEQTPLQRELDRVGKRLGLIV
                     IAIAVLMIGTILLVEEVRGVQAVFGVLILGVALAVAAVPEGLPAVVTTVLALGVRRMA
                     RRNAIVRHLSAVETLGSADIICSDKTGTLTRNEMTVRTIVTASGRVDFEGTGYAPEGA
                     IRSADGGAVEGDLHAELVRLLTAAERANNAALQQHDGRWTVQGDPTEGALIVAARKAG
                     LTAEALEARFERVAEVPFSSERKLMSTVHRDAERAERWLVFTKGAPDVLIARCAAEQV
                     GAGTRPLTDVRRREILARNEALAGEALRTLGVAYCAHPVDAFDPAAADDGVEADLVFC
                     GLIGMIDPPREEAGEAVARAKGAGIRPIMITGDHPKTAAVIAAELGITADGRAVTGVE
                     LARMSEEELARTVREVSVYARVDPEHKLRIVNALKRQGATVAMTGDGVNDAPALKTAD
                     IGVAMGITGTDVSRQAADMVLTDDNFASIVAAVEEGRGIFANIRKFLRYLLSSNIGEV
                     MTMFLGVLLADFIGLVPETHGAVALPLLATQILWINLVTDGPPALALGVDPADDGLMN
                     RPPRPRGQGVMTGRMWRGIFYVGMVMAAGTLLVLDASLPGGFIEGEGSLPHAQTMAFT
                     TLMMFQLFNVYNARSDERSAFHGLFRNVWLWLAVALSLALHGAVIYAPGLQQAFSTEP
                     LSAQDWLICAAVASSVLWLRELSKLVIPAGDAARRLEGATA"
     gene            4192..4911
                     /locus_tag="VKZ48_03695"
     CDS             4192..4911
                     /locus_tag="VKZ48_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HLU76993.1"
                     /translation="MKRHDRYVSGLALLYGAWWLALAVDPHYRDDWAVENVLVLLFVG
                     ALAATYRRFRLSRLSYTLIFVFLCLHAVGSHYTYAEVPYDAWFRSLTGHSLNALMGWE
                     RNHFDRFVHFMYGLLLVYPIREVFLRIADVRGFWGYFLPLDFAIASSAIYELIEWGAA
                     LVFGGELGAAYLGTQGDIWDAQRDMALASLGAFIAMAFTAAVNALLRRDFAEGVRESM
                     RVKHAQPLGEEELERLLRAKR"
     gene            4949..6778
                     /gene="ftsH"
                     /locus_tag="VKZ48_03700"
     CDS             4949..6778
                     /gene="ftsH"
                     /locus_tag="VKZ48_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HLU76994.1"
                     /translation="MDKQRQLNLWYFIGAFVLLLLFQSWWTTSQNVETIPYSDFIAYL
                     EQGRIAELQVTSDRIVGRLHQPINGKEYFATVRVDPALAEQMKDSGATFTGTIENTFL
                     SNLLSWVVPVLLFFGVWMFLFRRFAEKRMGGLVNVGKSKARVLVERDTGVTFDDVAGV
                     DEAKAELQEIVSFLKEKDKYGRLGARIPKGILLVGPPGTGKTLIAKAVAGQAGVPFFS
                     MSGAEFVEMFVGVGAARVRDLFEQARKAAPCIIFIDEIDALGKARSGFSWGGHDEKEQ
                     TLNQLLAELDGFDPREGIVLLAATNRPEILDPALLRAGRFDRQVVLDRPDRNGRAAIL
                     AVHVKKVRLAPEVDLAEVAALTPGFSGADLANLVNEAAIFATRRGADAVGMHDFTAAL
                     ERIVAGLERKGRVLHPGERKTIAYHEMGHALVAAAVPGTDPVHKVSIIPRSIGALGYT
                     MQRPTEDRFLLRRSELLNRMTVLLGGRAAEQLIFGEVSTGASDDLAKATDIARQIVTR
                     FGMSETLGQAVLERQRMTYLGDRQFGMQEKDYSEETAREIDVAVRELIDEAFRRALQI
                     LTERRAVLEEGATLLLEKETLTPKEFPPLAQKAPAEEGDEAAG"
     gene            complement(6779..8104)
                     /locus_tag="VKZ48_03705"
     CDS             complement(6779..8104)
                     /locus_tag="VKZ48_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HLU76995.1"
                     /translation="MRLARLKFVYGSIVLLITAAWLATAWPVSDAFWPMRRAFVMYTG
                     LLAIGSMSAAIVLAARPVQFEPLLGGLDKYYRLHKWLGIGAAMTALAHWLAVSAPRTM
                     AQLGWIEGPRRHRAANAAAGSDAFHALRESAADLGEWAFYLFLVLIVLALWKRVPYRH
                     FFRAHKLMAPVYLVLVYHSVVLMGREYWTAAAGPLMALLLAAGSTAAFASMFGRIGKS
                     RRAVGVIESLAHHRDNAVLDVSVRLDTAWPGHQAGQFAFVEFDRTEGAHPFTISSAWR
                     GDGRLMFGIKGLGDYTRTLPEQLRVGQAVTVEGPYGRFDFRDKGRRQIWIGGGIGIAP
                     FIAGLQQLASREPSANIDLYYSTDAPADDFIDNLRSLAQRAGVRFHLVLPQQEGRLTL
                     DRLAREVPEWQEAEVWFCGPKAFGDAIREAMSARGFNAARFHQELFDMR"
     gene            8514..10511
                     /locus_tag="VKZ48_03710"
     CDS             8514..10511
                     /locus_tag="VKZ48_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLU76996.1"
                     /translation="MLETQTVTAKGYAADALETPQAVDVIEGPGGASTGVAGDLLRGR
                     PGLALHSDGAWGQNPVLRGLKKESVVVLLDGVRVNSAQPQGAIASFLDLGLLDRAEVV
                     KGPTSVLYGSGAMGGVVNLITPEARFSSVSRFGGRIGLGASSVDRGLAGAALLNYSNP
                     DHALVLGAAGRDVEDYKAPDGRVDDTGYRSESFLLKYRHRLTDGLSLRFNVQHHTDRD
                     VWYPGSARTGGQPGGAGIPIPLGTVTIHSPEQQRELYEFGVDADVGPGKLSAEVYRQE
                     VFRQIRAWSANLERDYMRNDVSFDTNGARLRYVVPVGDKHVLTVGAETWQMKANPERY
                     VSNNPPLFDNNLRNDPFDDGRIESTGLFVQDEFDLGRTRIVAGARLDRTKGDAKQKGV
                     GPAAQTTGLDHTDSNVSWSLGAIRPLTAKLSVYGNIGRAYRAADMRERFEEAPRGDGF
                     FHVGNPQLDPERSTSFEVGVKGREGRLEYQLAAFHTRIDDYIAGRITGANHPVNGLPI
                     KQTENLDKVTIRGLEGGISMPLGVFVADAGFTWMRGKNHQDDEPLYQMPANELRLGLG
                     QPAERGFYWSGQLRMVAEQDRVAQKFSAGTEDKTAGFVTADANFGWRFGKAGGFSSTA
                     IDVRLTNLFDKRYHEHLTDGISGQEIDAPGRGIAVSLNGSF"
     gene            10513..11547
                     /locus_tag="VKZ48_03715"
     CDS             10513..11547
                     /locus_tag="VKZ48_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLU76997.1"
                     /translation="MQPSIAIRPSSCRSMWGRIAMLCVLLFAFGAAYAQKLPRLVLAG
                     PMASVSNPLIHMAESGALADIADQVEFQLWRDPDQLRVMALDGKADVLAMPVNVAANL
                     YNRGAKLRLLNVSTWGVLWMVSREEGLKTLADFRGKEIAMPFRGDMPDIIFNLLAEGQ
                     GIDVRKEIKLRYVATPVDAMQLLVMRRVDHALLAEPAVSMALRKTHSFPLSAIAPELY
                     RSVDLQQEWGRVFKREARIPQAGIALMASVKDRPDIVARIEAEYARSLEWCKANAARC
                     GELVAEHVDMLTPEAVADSVAVGQMQAVSARAARTEVEFLYEHLRRKTPALIGGKLPP
                     DDFYGGSAQQ"
     gene            11544..12335
                     /locus_tag="VKZ48_03720"
     CDS             11544..12335
                     /locus_tag="VKZ48_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLU76998.1"
                     /translation="MRLLRAWLQGLPAYLWSGWGAVASLLLFVAAWEAVAAYYGPLVM
                     PDPRSAFGTLYELIQSGAAWPELAATARRALIGFTLALVVGSVLGLIAGVSMTASMMS
                     RPLVTVLLGTPPIAWLVLAMLWFGASDGTPVFTVFVASFPVVFVGALQGARTLDNNLR
                     TMAEAFRVPRRMMFVDVYLPHVVSYLFPAWIAALGISWKVVVMAELLSTQDGVGAALA
                     VSRSHLDTASTMAWIVAVVGLLLAVEYLLLEPIKREVERWREAAT"
     gene            12332..13180
                     /locus_tag="VKZ48_03725"
     CDS             12332..13180
                     /locus_tag="VKZ48_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLU76999.1"
                     /translation="MSAAQLIVRGVSHAFARNEVLADIDFSVQAGQVVALVGPSGCGK
                     TTLLNLCAGLLHVREGAIENGFAEAAYMFQQPRLLPWKTTLDNIALGLKAAGMARGER
                     HYRARELALRLGLVHEDLDKFPHQLSGGMQSRVALARALVLQPDLLLLDEPFSALDIG
                     LKAELYELLLAQLAERDMGVLMITHDLMEAVRLSDLILVMTAAPGRIVCRFELSRPAA
                     QRDDAWVYRRTAELLQDATVRESFALPPLAVAAPLVNDEGDGELRRLDAPSSVLPFPR
                     ARAGCR"
     gene            13180..14466
                     /locus_tag="VKZ48_03730"
     CDS             13180..14466
                     /locus_tag="VKZ48_03730"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HLU77000.1"
                     /translation="MRVPDWFDRLAAHPLFLCGFRPLFFAAATYAVLALLSWLGFLAA
                     GIALPSVPGGPVAWHAHELLFGFGLAAVTGFVLTAIPEFTGTASFGSRIALAFSLLWL
                     AGRVLFWSSGVIGVWPAALAHIGLVVWLVALLAPRILRDPELRHLDFLLWGLAPLALT
                     VIGFYVDALRGDYPMRWLHAAIGVMMLLIVLAMSRISMRIVNDALEALRERGMDAGNY
                     YSRPPRRNLALFAIAVYTAAEFFVPHTAFSGWLGLVAAAALFNQLNDWHVGRALFDRW
                     SAMGYAMYWLMALGYAALGLSALDAGIAPSAGTHLLTVGAMGLSTLAVLCIAGRIHAG
                     YVADPRSWVLFAATLLVIAALLRALSGFAGEHAALLLMLSGLGWVAAFLLATFYLGGG
                     WLRPRVDGGIGCEEVREGEAAVEVVHAFRVQGPEHE"
     gene            14463..14708
                     /locus_tag="VKZ48_03735"
     CDS             14463..14708
                     /locus_tag="VKZ48_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77001.1"
                     /translation="MKQRKSPAASAFFSHLPAGFFGRFAISSSWKGGAPLRPALPPRL
                     MLRESHAPSCGVARCDGASTWECTLFARPRFLFKSEG"
     gene            15153..16253
                     /gene="dctP"
                     /locus_tag="VKZ48_03740"
     CDS             15153..16253
                     /gene="dctP"
                     /locus_tag="VKZ48_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HLU77002.1"
                     /translation="MTQNRRGFLKKSVVAGAAAGTLGFPMVAKAQSKFQWKMTTTWPA
                     GTPFYQSGPGSAEAFAKRVADMSNGRLTIKVFAAGELLPAFEGFDACSAGVVEMNHGV
                     AYYWAGKTFASQYFGTVPFGMSFNGQHAWLQFGGGNDLWREIYKPFKVLPFAIGCSGV
                     QMTGWFKREVKSVDDFKGLKMRIPGLAGRAYAELGVDVKLLPGGEIFPALERGVIDAA
                     EWVGPYLDHRLGLQNAAKYYYTTGWHEPSTTSELVVNEAAYNKLPKDLQAIVKAAADA
                     TNQEGLLLLEARNAAALDELVNKYGVKISRLPEDVIKRLREVSMDIFSEAAAKDAMVK
                     KVHDSYFAFKKTHDHWNQISETTFQTTVRGLG"
     gene            16348..16890
                     /locus_tag="VKZ48_03745"
     CDS             16348..16890
                     /locus_tag="VKZ48_03745"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HLU77003.1"
                     /translation="MKNKLIKTADGIDRFIDWTGLFAAWLCLVLVLLIAVEVILRYTM
                     SLGAVWAQELEWHLLAVIALWGISYTQKEDAHVRVDILYQNFSQRTKDWLEFFSTLLV
                     MVPLSFYVAWLAWDFMAQAYRIGEISPDPGGLTHRWVLKSFVMTGFILLGIQSTALML
                     RSAARVFFNNNNDNGGEHAA"
     gene            16880..18211
                     /locus_tag="VKZ48_03750"
     CDS             16880..18211
                     /locus_tag="VKZ48_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HLU77004.1"
                     /translation="MPLNEIYAIGMLLSFVALLLAGVRVAYAIAASGFIFGLIGFGPL
                     LFNLLPARMYGVATNYTLLAVPLFVFMGVMLEKSHIAEDMLDVMGHLAGRVNGGMGVA
                     IILVGMMMGAATGIVGATVVTLGLIALPTLLRRGYNTGLATGSICASGTLGQIIPPSL
                     ILILLSDLMGESVGTLFAAALMPGLMLAALYVLYILGLGLIRPDLAPALPKEERSRVT
                     GRELFKRTLLGVVPPIALVFAVLGSIIGGIAAPTEAAAMGAAGSILVVAALGKLNNKV
                     MSETMRDTLKTTAMIFFIVMASQVFSLAFRGLSGEDLIAEFMGAIPGGIWGAIIFMML
                     LLFILGFFLEWIEISYIVLPLLLPIFVAADVNMVWLAMLICMNLQTSFLTPPFGWALF
                     FMKGCAPPGVKTGDIYKGVIPFIGIQIVGLTLLALFPQIALWLPTAIGWMK"
     gene            18270..>19101
                     /locus_tag="VKZ48_03755"
     CDS             18270..>19101
                     /locus_tag="VKZ48_03755"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014193194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HLU77005.1"
                     /translation="MQNTACGVRGIAVAPHALAAQSAIAVLREGGNALEAMIAAAATI
                     AVVYPHMNGIGGDAFWLLSAPGAPVRAIDASGAAGRSVSRALYADHGLDTIPMRGPLA
                     SNTVAGTISGWNKALEIGAEWGGRLPLSRLLGDAIHYARDGIPVTASQAASTQAKLAE
                     LATQPGFAETFLIDGASPQAGSVFLQPRLAEVLEQLVRAGLDDYYRGDLARRIAGDLA
                     TIGSPLTLEDLNAHRALEVRPLALRHSLGTVYNMTPPTQGVISLAILGVADRLGLDKL
                     D"
BASE COUNT         3086 a         6063 c         6427 g         3525 t
ORIGIN      
        1 ccatcgtcaa cgaggagatc gccaccttca tgcaggggcg caaagagacg acgccgctgc
       61 cggtggaact ggccgtgcgc gtgcgcttca atccggagct caacaaatcc tggttcggcg
      121 ccgtgatcca ggtgatcaac caggtgacga tgctctctat catcctcacc ggcgcggcgc
      181 tgctgcgcga gcgcgagcac ggcaccgtcg agcatctgct cgccatgccg gtgacgccgt
      241 tcgaaatcat gaccggcaag ctgtggtcga tgggactcgt cgtactcctc gccacgggga
      301 tctcgctggt gacggtggtg cagggggcgc tgggtgtgcc catcggcggc tcgatggcgc
      361 tgtttctgtg cggcgcggcc ttgaacctgt tcgccacgac ctcgctgggg atttttctcg
      421 ccaccatcgc gcgctcgatg ccgcagttcg gcctgctgct gatcctcgtg ctgctgccgc
      481 tgcagatcct gtccggcggc gcgacgccgc gcgagagcat gccggaaatc gtccaggtcg
      541 tgatgctggg cgcgccgacc acgcatttcg tgctgctggc ccaggcgatc ctgtttcgcg
      601 gcgccggctt cgacatcgtc tggccgcagt tcgtcgcgct ggcgctgatc gggacggcgc
      661 tgttcgtcta ttcgctgacc cgctttcgcc gggccattgc gtcgatggcc tgagcggcat
      721 acggtgcgcg atactgacgg cgttcatcga tacaccggag caaatcatga aacccctgca
      781 actcggactt tccctgacgc ttgccgccgc gctgacatgc gccgcgccgg ctgtcgccgc
      841 agaacccttc gcgccctatg gcaccgccaa ggtcgacatg cacgacatgc cggtgaccaa
      901 cgtcgtgttc gacgtcaatt atcaggaccc gaagagcctg gacattctgt accacttcat
      961 ccgcaacacc tggaaggaga ccaagggcaa ggtggtggtg gtgagccacg gtccggaatt
     1021 gcgcgctttc gccaaggaga actacgagaa gtatcaggcc gtggtcgatc agcttgccga
     1081 actggcgcag gagggcgtcg agttcaagat gtgcaacaac gccttgcgcg cggcgggctt
     1141 tcagcctgaa gacatgcatg gtttcgtgac ggtcgtgccg gcaggtttcc cggaactggc
     1201 ctactggcag agcaagggtt atcagtacat caacccgctg cccttgccgg tgggaggggt
     1261 gcgttatctc gatcagccgc agttgaagaa gccttgaacg catgcgagat gagccgggtc
     1321 gggcatcgtg cccgaatacg ctttcgatga ggtttccgtg tcaggcgtga acgatcgcga
     1381 cactgccgca gcctcggcgc atacgcagtc ggtggaggag attgcgcagg cgctgcgcag
     1441 cgacccggcg tgcgggttga cgcaacgcga ggcgcaggcg cgcctgcggc acagcggtcc
     1501 caacgaactg gcacacgaaa aacccttgcc ggcatggcgc aagttccttg cgcagttcgg
     1561 caacgtgctg gtcatcctgc tgctcgttgc cgccgccatt tctgccctgc tgtgggcgat
     1621 cgagcccgac gcggccttgc cctatgaagc catcgcgatc ctggtgatcg tgctgctcaa
     1681 cgcattgatg ggctatatcc agcaggcgcg cgccgagcgg gcagtggcgg cattgaagca
     1741 gatgtcggcc gcgcatgcga cggtgatccg cgacggcgaa cggcggcgca ttccggccgc
     1801 cgaactcgtg ccgggcgacg tgatcctggt cgaggaaggc gacaccgtgc ccgccgatgc
     1861 gcgcgtggtc gaatccaccg cgctgcagac ggccgaagcg gcgctgaccg gtgaaagtct
     1921 gccggtggcg aaggacaccg agccggtcgc ggcgcaggcg ccgctcggcg atcgcgccgg
     1981 catgatctat agcggcacgg cggtgactta cgggcgcggc cgcgccatcg tcacggcgac
     2041 gggcatgcgc acggagatgg ggcgcatcgc cggcatgctc gaagcggcgc cggccgaaca
     2101 gacgccgctg cagcgcgaac tggatcgcgt gggcaagcgc ctcggactga tcgtcatcgc
     2161 catcgccgtg ctgatgatcg gcacgatcct gctggtggag gaggtgcggg gcgtgcaggc
     2221 ggtgttcggc gtgctcatcc tcggcgtcgc gcttgcggtg gccgccgtgc cggagggctt
     2281 gcccgccgtc gtgaccaccg tgctcgcgct cggcgtacgc cgcatggcgc gccgcaatgc
     2341 cattgtgcgg catctttcgg cggtggagac gctgggttcg gccgatatca tctgctccga
     2401 caagaccggc acgctgacgc ggaacgaaat gacagtgcgc accatcgtca cggcgagcgg
     2461 tcgggtggat ttcgaaggca ccggttatgc gccggagggt gcgatacgca gtgccgacgg
     2521 cggcgcggtc gaaggcgatc tgcatgccga actggtgcgg ctgctgacgg cggccgagcg
     2581 cgccaacaac gcggcgctgc aacagcatga cggtcgctgg acggtgcagg gcgacccgac
     2641 cgaaggcgcg ctcatcgtcg ccgcgcgcaa ggcgggcctt acggccgaag cgctcgaggc
     2701 gcgcttcgaa cgcgtggccg aggtgccgtt ctcctccgaa cgcaaactga tgagcacggt
     2761 gcatcgcgat gcggagcgtg cggagcgctg gctcgtgttc acgaagggcg cgcccgacgt
     2821 gctcatcgcc cgttgtgccg ccgagcaggt cggcgcgggc acacgtccgc tgaccgatgt
     2881 gcggcggcgg gaaatcctcg cgcgcaacga agcgcttgcc ggcgaggcac tgcgcaccct
     2941 cggcgtcgcg tattgcgcgc atcccgtcga cgccttcgat cccgcggcgg cggacgacgg
     3001 cgtcgaagcg gatctggtgt tctgcggact catcggcatg atcgatccgc cgcgcgagga
     3061 ggccggagaa gcggtcgcac gtgcaaaggg cgcaggcatc cgcccgatca tgatcaccgg
     3121 cgaccatccc aagaccgccg cggtgatcgc cgccgaactg ggtatcaccg cagacggccg
     3181 ggcggtcacc ggcgtcgaac tcgcacgcat gagtgaggag gaactggcgc gcacggtgcg
     3241 cgaggtatcc gtctatgcgc gggtcgatcc cgaacacaag ctgcgcatcg tcaatgcgct
     3301 caagcggcag ggcgcgaccg tggcgatgac cggcgacggt gtgaacgacg caccggcgct
     3361 caagacagcg gacatcggcg tggccatggg catcaccggc acggacgtgt cgcggcaggc
     3421 agcggacatg gtgctcaccg acgacaactt cgcgtccatc gtcgcggcgg tggaggaggg
     3481 gcgcggcatc ttcgccaata tccgcaagtt cctgcgctat ctgctgtcct cgaacatcgg
     3541 cgaagtgatg accatgttcc tgggcgtgct gctggccgac ttcatcggcc ttgtgccgga
     3601 gacgcatggc gccgtcgcgc tgccgctgct ggccacacag atcctgtgga tcaatctcgt
     3661 taccgacggc ccgcccgcac tggcgctcgg cgtcgatccc gccgacgacg ggttgatgaa
     3721 tcgtccgccg cgtccgcgcg gccaaggggt gatgaccgga cgcatgtggc ggggcatctt
     3781 ctacgtcggc atggtgatgg ccgccggcac cctgctggtg ctcgatgcct cgctgcccgg
     3841 cggcttcatc gagggcgagg gcagtctgcc ccatgcgcag accatggcct tcaccacgct
     3901 gatgatgttt cagcttttca atgtctacaa tgcgcgttcg gacgaacgca gcgccttcca
     3961 cggtctgttc cgcaacgtct ggctgtggct ggccgtcgcc ctgtcgctcg cgctgcacgg
     4021 cgcggtgatc tacgcgcccg gtctgcagca ggcgttctcg accgagcccc tgagcgcgca
     4081 ggactggctc atctgtgcgg cggtcgccag cagcgtgctg tggctgcgtg aattgagcaa
     4141 gcttgtcatt ccggccggcg acgccgcgcg ccgcctcgag ggagcgaccg catgaagcgg
     4201 catgatcgtt atgtgtcggg actcgcgctg ctctacggcg cgtggtggct cgcgctggcg
     4261 gtcgatccgc actaccgcga cgactgggcg gtggaaaacg tgctggtgct gcttttcgtc
     4321 ggcgcgctgg ccgcaaccta ccgccgcttc cggctgtcgc ggctctccta tacgctgatc
     4381 ttcgtctttc tctgcctgca tgcggtgggt tcgcactaca cctatgcgga agtgccctac
     4441 gacgcgtggt tccgctcgct caccggccac tcgctcaatg cactcatggg ctgggagcgc
     4501 aaccacttcg accggttcgt gcatttcatg tacggtctgc tgctggtcta tcccatccgc
     4561 gaagtgtttc tgcgcattgc cgacgtgcgc ggcttctggg gctatttcct gccgctcgat
     4621 ttcgccatcg ccagctcggc gatctacgaa ctgatcgaat ggggcgccgc gctggtgttc
     4681 ggcggcgaac tgggcgcggc ctatctgggc acgcagggcg acatctggga tgcgcagcgc
     4741 gacatggcgt tggcgagcct cggtgcgttc atcgcgatgg cgttcactgc cgcggtcaac
     4801 gcgctgttgc ggcgcgactt cgccgagggc gtgcgcgaga gcatgcgcgt caagcatgcg
     4861 cagccgctgg gcgaagagga actcgaacga ctgctgcgcg ccaagcgctg atttcggatt
     4921 gatcggatac ggttgaagga acgttcgtat ggacaagcag cgacaattga atctctggta
     4981 cttcatcggc gcctttgtat tgttgttgct gttccagagc tggtggacca cttcgcagaa
     5041 cgtcgagacg attccgtata gcgacttcat cgcctatctg gagcagggac gcatcgccga
     5101 actgcaggtg acgagcgatc gcatcgtcgg ccgtctgcat cagccgatca acggcaagga
     5161 gtacttcgcc accgtgcgcg tcgatccggc gctcgccgag cagatgaagg acagcggcgc
     5221 gaccttcacc ggtacgatcg agaacacctt cctcagcaac ttgctgtcgt gggtcgttcc
     5281 cgtgctgctg ttcttcggcg tgtggatgtt cctgttccgc cgctttgccg agaagcgcat
     5341 gggcggcctg gtcaacgtgg gcaagtccaa ggcgcgggtg ctggtcgagc gcgacaccgg
     5401 cgtcaccttc gacgatgtcg ccggcgtgga cgaggccaag gcggagctgc aggagatcgt
     5461 gtccttcctc aaggagaagg acaagtacgg ccggctgggc gcgcgcattc ccaaaggcat
     5521 cctcctcgtc gggccgccgg gcaccggtaa gacgctcatc gccaaagcgg tggccggaca
     5581 ggcgggcgtg ccgttcttca gcatgtccgg cgccgagttc gtcgagatgt tcgtcggcgt
     5641 gggcgcggcg cgcgtgcgcg atctgttcga acaggcgcgc aaggctgcgc cgtgcatcat
     5701 tttcatcgac gaaatcgacg cgctcggcaa ggcgcgcagc ggtttcagct ggggcggtca
     5761 cgacgagaag gagcagaccc tcaaccagtt gctcgccgaa ctcgacggct tcgatccgcg
     5821 cgaaggcatc gtgctgctcg ccgcgaccaa ccggccggag attctcgatc cggcgctgct
     5881 gcgcgcgggg cgctttgacc gtcaggtcgt gctcgaccgt cccgaccgca acgggcgcgc
     5941 cgccattctg gcggtgcacg tgaagaaggt tcgtctggcg ccggaggtcg atctggccga
     6001 agtggccgcg ctcacgcccg gcttcagcgg tgccgacctg gccaacctgg tgaacgaagc
     6061 ggctatcttc gccacgcgcc gcggtgcgga cgcggtcggc atgcacgact tcaccgcggc
     6121 gctggagcgc atcgtcgccg gtctggagcg caagggccgg gtgctgcatc cgggggagcg
     6181 caagaccatc gcctaccacg agatggggca tgcgctggtc gccgccgcag tgccggggac
     6241 cgatccggtg cacaaggttt cgatcattcc gcgttccatc ggcgcgttgg gctacaccat
     6301 gcagcgcccg accgaagacc gcttcctgct ccggcgcagc gaactgctca accgcatgac
     6361 cgtgctgctg ggcggccgcg ccgccgaaca actgatattc ggcgaggtct cgaccggcgc
     6421 gtccgacgat ctggcgaagg ccaccgatat tgcgcgacag atcgtgacgc gcttcggcat
     6481 gagcgagacg ctcggccagg cggtgctgga acgtcagcgc atgacctatc tgggcgaccg
     6541 gcagttcggc atgcaggaga aggattactc cgaggaaacc gcccgcgaaa tcgatgtggc
     6601 cgtgcgcgaa ctgatcgacg aggcattccg gcgcgcactg cagattctca ccgaacgccg
     6661 cgcggtgctg gaggaaggcg cgacgctgct gctcgaaaaa gagacgctga cgccgaagga
     6721 gttcccaccg cttgcgcaaa aggcacccgc ggaggaaggc gacgaagccg cggggtgatc
     6781 agcgcatgtc gaacagttcc tgatggaagc gtgcggcgtt gaagccgcgc gccgacatcg
     6841 cttcccgtat cgcgtcgccg aaggctttcg gtccgcagaa ccacacctcc gcctcctgcc
     6901 attcgggcac ctctcgcgcg agacggtcga gtgtcagccg cccttcctgt tgcgggagca
     6961 cgagatggaa gcgcacgccg gcacgttgcg cgaggctgcg gaggttgtcg atgaagtcgt
     7021 cggccggcgc atcggtggaa tagtagagat cgatattcgc tgacggctcc ctgctggcca
     7081 gttgctgcag gccggcgatg aacggggcga tgccgatgcc gccgccgatc cagatctgac
     7141 gccgcccctt gtcgcggaaa tcgaaacggc cgtaggggcc ttcgaccgtt accgcctgac
     7201 ctacgcgcaa ttgctcgggc agcgtgcgcg tgtaatcccc cagtcccttg atgccgaaca
     7261 tcaggcggcc gtcgccacgc catgcggagg agatggtgaa cgggtgtgcg ccttcggtgc
     7321 gatcgaattc gacgaaggcg aactggcccg cctggtggcc cggccatgcg gtatcgagac
     7381 gcactgacac gtcgagcacg gcgttgtccc gatgatgcgc gagcgattcg atgacgccga
     7441 cggcgcgtcg cgacttgccg atgcggccga acatcgaggc gaacgccgcg gtgctgccgg
     7501 cggcgagcag gagcgccatg agcgggccgg cagcggcagt ccagtactcg cgccccatca
     7561 agacgaccga gtggtacacc agcacgaggt aaacaggcgc catgagtttg tgggcgcgga
     7621 agaaatgccg atagggcacg cgcttccaca gcgcgagcac gatcaggacg aggaacaggt
     7681 agaacgccca ttcgcccaga tcggcggccg actcgcgcag cgcgtgaaag gcgtcggacc
     7741 cggcggctgc gtttgcggca cgatggcgcc gcggcccctc gatccagccc agttgcgcca
     7801 tggtgcgcgg tgcgctcacc gccagccagt gcgcgagcgc cgtcattgcc gcgccgatgc
     7861 cgagccactt gtgcaagcgg tagtacttat ccaatccacc gagcagcggc tcgaattgca
     7921 ccgggcgcgc ggcgagcacg atggccgccg acatcgatcc gatcgcgagc agcccggtgt
     7981 acatcacgaa cgcgcgacgc atcggccaga acgcgtcgct caccggccac gcggtcgcaa
     8041 gccatgctgc cgtaatgagc agcacgatgg aaccgtagac gaacttcagc cgagcgagtc
     8101 gcatgccata caggctagcc gaatgcgcgg cggggaggct gacctgcgtc aatgcgtatt
     8161 gcggtgacga ttgcggcgcg atgcgcgtcg tgtgcatcgc aaagcccttc ggtgcgttcg
     8221 attaacaacg ctgaaaaccc ttcgcatcgc attggttaaa acgatctgca tcacgcattg
     8281 cgcgatgcgc gtgcttcatt tcgcgcggcg tttttgacgt cgatcaactg agaaccattc
     8341 tcgttggcta gcatggcttc aacgatttga ccaaccaacg ggcaagccag gccgcaccca
     8401 gccagcccat cgacgatgga gccctgactg tgttgtcttt acgcccgctt gccgtggctt
     8461 gcctcggtgt gttcgccgtg cctgtactac aaaccgctca ggcggaggag accgtgcttg
     8521 aaacgcagac ggtcaccgcc aagggctatg ccgccgatgc gctggaaaca ccgcaggcgg
     8581 tggacgtgat cgaaggtccc ggcggtgcga gcaccggcgt ggcgggcgat ctgctgcgcg
     8641 gacgccccgg tctcgcgctg cattccgacg gcgcatgggg gcagaacccg gtgctgcgcg
     8701 gattgaagaa ggagagcgtg gtcgtgctgc tcgacggcgt gcgcgtgaac tcggcgcaac
     8761 cgcagggcgc catcgcgtcc tttctcgatc tcggtctgct cgatcgtgcc gaggtggtga
     8821 agggcccgac ctcggtgcta tacggcagcg gtgcgatggg cggcgtggtc aacctcatca
     8881 cgcccgaagc gcgcttcagc agcgtaagcc gcttcggcgg acgcatcggc ctcggtgcga
     8941 gcagcgtcga tcgcggtctt gcgggcgcgg ccctgttgaa ctattccaat cccgatcacg
     9001 cgctggtgct cggtgccgcc ggccgcgacg tcgaggatta caaggcgccg gacggccgcg
     9061 tggacgacac cggctaccgt tccgaatcct ttttgctcaa gtatcgtcat cgcctcaccg
     9121 acggcctgag cctgcgtttc aatgtgcagc atcacaccga tcgcgacgtg tggtatcccg
     9181 gctcggcgcg taccggtggt cagccgggcg gcgcggggat tccgataccc ctgggcaccg
     9241 tcaccatcca ttcgccggaa cagcagcgcg agttgtacga gttcggcgtc gatgccgacg
     9301 tcggacccgg caagctgagc gcagaggtct atcgtcagga agtgttccgt cagattcgtg
     9361 cctggtcggc caacctggag cgggattaca tgcgcaacga cgtgagcttc gacaccaacg
     9421 gtgcgcgtct gcgctacgtc gtcccggtcg gcgataagca cgtgctcacg gtcggcgcgg
     9481 aaacctggca gatgaaggcc aaccccgaac gctatgtgag caacaatccg ccgctgttcg
     9541 acaacaacct gcgcaacgat cccttcgacg acgggcgtat cgaatccacc ggcctgttcg
     9601 tgcaggacga attcgacctg ggccgcacgc gcatcgtcgc cggtgcgcgc ctcgatcgca
     9661 ccaaggggga tgcgaagcag aagggcgtgg gtcccgccgc gcagaccacc ggcctcgatc
     9721 acaccgacag caacgtatcg tggtccctcg gcgccatccg tccgctcacc gccaagctca
     9781 gcgtgtacgg caatatcggc cgcgcctatc gcgccgccga tatgcgcgag cgcttcgagg
     9841 aagcgccgcg cggcgacggc ttcttccatg tcggcaaccc gcaactcgat ccggaacgct
     9901 cgaccagttt cgaagtcggc gtgaaaggac gcgaagggcg gctcgagtat caactggcgg
     9961 ctttccacac ccgcatcgac gactacattg ccggacgcat caccggcgcg aatcatccgg
    10021 tgaacggcct gcccatcaag cagaccgaga acctcgacaa ggtcaccatc cggggtctgg
    10081 aaggcggcat cagcatgccg ctgggcgtat tcgtcgcgga cgcgggcttc acctggatgc
    10141 gcggcaagaa ccatcaggac gacgaaccgc tgtaccagat gcccgccaac gaactgcgcc
    10201 tgggtctggg ccagcccgcc gagcgcggct tctactggag cggccagttg cgcatggtgg
    10261 cggaacagga ccgcgtcgcg cagaagttct ctgccggcac ggaagacaag accgccggct
    10321 tcgtcaccgc cgacgccaat ttcggctggc gtttcggcaa ggcgggcggt ttttcgtcca
    10381 ccgccatcga cgtgcgtttg accaacctgt tcgacaagcg gtatcacgaa catctcaccg
    10441 acggcatttc cgggcaggag atcgacgcgc ccgggcgcgg catcgccgtc agcctgaacg
    10501 ggagcttctg acatgcaacc atccatcgcg atccgtccct cgtcctgccg ttccatgtgg
    10561 gggcgcatcg cgatgctgtg tgtgctgctc ttcgccttcg gcgcggcgta tgcccaaaag
    10621 ctgccgcgcc tcgtgctcgc cggaccgatg gcctcggtgt cgaacccgct catccacatg
    10681 gccgagtccg gcgcgctggc cgatatcgcc gatcaggtcg agttccaact gtggcgcgat
    10741 cccgatcagt tgcgcgtcat ggcgctcgac ggcaaggccg acgtgctggc gatgccggtg
    10801 aatgtcgcgg ccaatctcta caaccgcggc gcgaagttgc gtctgctcaa cgtctcgacc
    10861 tggggcgtgc tgtggatggt gtcgcgcgaa gaagggttga agacgctcgc cgatttccgc
    10921 ggcaaggaaa tcgccatgcc gttccgcggc gacatgccgg acatcatttt caacctgctc
    10981 gccgaagggc agggcatcga cgtgcgcaag gagatcaagc tgcgctatgt cgccacgccg
    11041 gtggatgcga tgcagttgct ggtgatgcgc cgtgtcgacc acgcgctgct ggccgaaccg
    11101 gcggtgtcga tggcgctgcg caagacccat tcctttccgc tcagcgcgat cgcgccggaa
    11161 ctgtaccgca gcgtcgatct gcagcaggaa tgggggcgcg tgttcaagcg cgaggcgcgc
    11221 attccgcagg ccggcattgc gctgatggcg agcgtgaagg accggcccga catcgtcgcg
    11281 cgcatcgaag ccgagtacgc gcgctcgctc gaatggtgca aggccaacgc cgcccgatgc
    11341 ggcgaactgg tggccgaaca cgtggacatg ctcacccccg aggccgtcgc cgattcggtc
    11401 gccgtgggac agatgcaagc cgtgtccgcg cgcgcggcgc gtacggaagt ggaattcctc
    11461 tacgaacatt tgcggcggaa aacgcctgca ctgatcggcg gtaaactgcc tcccgacgac
    11521 ttctacggag ggagtgcaca acagtgaggc tcttgcgcgc ttggctgcag ggattgccgg
    11581 cctatttatg gagcggctgg ggcgcggtcg cgagtctgct gttgttcgtc gcggcgtggg
    11641 aggcggtggc cgcgtactac ggaccgttgg tcatgcccga tccgcgctcg gctttcggca
    11701 cgctgtacga actgatccag agcggtgcgg cctggccgga actggccgcc acggcgcgcc
    11761 gcgcgctgat cggtttcacc ctggcactcg tcgtcggcag tgtgctgggc ctcattgcgg
    11821 gcgtatcgat gacggcgtcg atgatgtcgc ggccgctcgt caccgtgctg ctcggcacgc
    11881 cgcccatcgc ctggctggtg ctggcgatgc tgtggttcgg cgcgagcgac ggcacgccgg
    11941 tgttcaccgt cttcgtcgcg agttttccgg tggtcttcgt cggcgcgctg cagggcgcgc
    12001 gcacgctcga caataatctg cgcaccatgg ccgaagcctt ccgcgtgccg cggcgcatga
    12061 tgttcgtcga tgtttatctg ccgcacgtgg tgtcctatct ctttcctgcg tggatcgcag
    12121 cgctcggcat ctcctggaaa gtggtggtga tggccgagct gctgtccacg caggacggcg
    12181 tgggtgcggc gctcgcggtg tcgcgctcgc atctggatac cgcttcgacc atggcgtgga
    12241 tcgtcgccgt ggtcggtctg ttgctcgcag tcgagtatct gctgctcgaa ccgatcaagc
    12301 gcgaagtcga gcgctggcgc gaggcggcga catgagtgcg gcgcagctca ttgtgcgcgg
    12361 tgtatcgcac gccttcgcgc gtaacgaagt cctggcggac atcgacttca gcgtgcaggc
    12421 ggggcaggtc gtcgcgctgg tcggcccctc gggttgcggc aagaccacgc tgctcaatct
    12481 ctgcgcgggt ctgctgcatg tgcgcgaggg tgcgatcgaa aacggtttcg ccgaagcggc
    12541 ctatatgttc cagcagccgc gcctgctgcc gtggaagacg acgctcgaca atattgcact
    12601 gggtcttaag gccgccggca tggcgcgcgg ggagcggcat taccgcgcgc gcgagttggc
    12661 gctgcgtctc ggtcttgtgc acgaagatct cgacaagttt ccgcaccagc tttccggcgg
    12721 catgcaaagc cgcgtggcgc tggcgcgcgc gctggtgctg cagcccgatc tgctgctgct
    12781 ggacgaaccc ttttccgcac tcgacatcgg cctcaaggca gaactgtacg agttgctgct
    12841 cgcccaactg gccgagcgcg acatgggcgt gctgatgatc acgcacgatc tgatggaggc
    12901 ggtgcggctg tcggatctca tcctggtgat gacggccgca cccgggcgca tcgtctgccg
    12961 cttcgaactg agccggccgg ctgcgcagcg cgacgacgca tgggtttatc gccgtaccgc
    13021 cgaactgttg caggacgcca ccgtgcgcga gagctttgcg ttgccgccgc tggcggtcgc
    13081 agcgcccctt gtcaacgatg agggcgacgg cgaattgcgg cgcctcgatg caccgtcgtc
    13141 ggtgctgccc tttccgcgtg cgcgagcggg gtgccgttga tgcgtgtgcc ggactggttc
    13201 gatcgactcg ccgcgcatcc gctgttcctc tgcggcttcc gtccgttgtt cttcgctgcg
    13261 gcgacctatg ccgtgctcgc cctgctgtcg tggctgggct tcctcgccgc cggcatcgcg
    13321 ttgccgagcg tgccgggcgg gccggtggcc tggcatgcgc acgaactgct gttcggcttc
    13381 ggtttggctg ccgtgaccgg cttcgtgctc acggcgattc cggaattcac cggcaccgcc
    13441 tccttcggtt cgcgcatcgc gctcgccttc agcctgctgt ggctggccgg gcgcgtgttg
    13501 ttctggtcct cgggcgtgat cggcgtgtgg ccggctgcgc tcgcgcacat cggcctggtc
    13561 gtgtggctgg ttgcgctgct tgcgccgcgc atcctgcgcg atcccgaatt gcggcacctg
    13621 gattttctgc tctggggcct ggcgccgctc gcgttgacgg taatcggctt ctatgtcgat
    13681 gcactgcgcg gcgactatcc gatgcgctgg ctgcatgcgg ccatcggcgt catgatgcta
    13741 ctcatcgtgc tggccatgag ccgcatctcc atgcgcatcg tcaacgatgc actcgaggcg
    13801 ctgcgcgaac gcggcatgga tgcgggcaac tattattcgc gaccgccgcg ccgcaatctg
    13861 gcgttgttcg ccatcgccgt ctatacggcg gcggagttct tcgtgccgca taccgccttc
    13921 agcggctggc tggggctggt ggcggccgcg gcgctgttca accaactcaa cgactggcat
    13981 gtgggccgcg cgctgttcga ccgctggagt gcgatgggct atgcgatgta ctggctgatg
    14041 gcgctgggtt atgcggcgct gggtctgtcg gcgctcgatg cgggtatcgc gcccagtgcg
    14101 ggcacgcatc tgctcaccgt cggtgcgatg ggtctttcga cgctcgccgt gctgtgcatt
    14161 gccggccgca tccacgccgg ttatgtggcc gatccgcgca gttgggtatt gttcgccgcg
    14221 accctcctcg tcatcgcggc gctgctgcgc gcgctctccg gtttcgccgg tgagcatgcg
    14281 gccttgctgt tgatgctgag cggactgggc tgggtcgccg ccttcctgct ggcgaccttt
    14341 tatctcggcg gcggttggct gcgtccgcgc gtcgatggcg gcatcggctg cgaggaagtg
    14401 cgcgagggtg aagcggcggt tgaagtggtg catgcgttcc gggtgcaagg cccggaacat
    14461 gaatgaagca gcgaaaaagc ccggccgcga gcgccttttt ctcccatctc ccggccggct
    14521 tcttcggccg gtttgcgatt tcttcgtcct ggaagggcgg ggcgccgctc cgaccggcct
    14581 tgccgccgcg cctgatgttg cgtgaaagcc atgcaccgtc ctgcggcgtg gcgcgctgcg
    14641 acggtgcttc gacgtgggag tgcacgctgt tcgcgcgccc gcgattcctt ttcaaatccg
    14701 agggttgaca gttcttgcac gcaacctgct tcaaggcctg ggcgcaaagg gagtgagacg
    14761 agttgccgta tcggaaagaa cagcgtgcac aaagtgtccg acacgtcttg catattagtt
    14821 gtgcgatttg catactagac gccaagttgt gcagggcaac aagaacccgc atcaagcgaa
    14881 gcacaaccaa aacggggtca ggcagtcgtg ccggacccta caaaaaagag gaggtgcatg
    14941 cgtgtgttcc gtctcttcct gcggtccgcc cttccggtgt cgttcgatcc ggcgaccgat
    15001 tcgctttcct gtttttccta caactgatca accgtcgtgc tcaggcatgc ggagcgcgcg
    15061 tgcgcgctct tcgcacggcg gcgtcaggcg tgcagagcgt cacctccatc agagcggcat
    15121 tcacttgtcc taaataagca aggagagaag caatgactca aaatcgacgc ggttttctca
    15181 agaagagtgt ggttgccggg gctgcggccg gaacgcttgg ctttcccatg gttgccaagg
    15241 cgcagagcaa gtttcaatgg aagatgacga ccacgtggcc ggccggcacg ccgttctatc
    15301 agtcggggcc cggcagcgcc gaggccttcg ccaagcgcgt ggccgacatg tcgaacgggc
    15361 gcctgacgat caaggtgttc gccgcgggtg aactgctgcc ggccttcgaa ggcttcgatg
    15421 cctgctcggc cggcgtggtg gaaatgaacc acggcgttgc ctactactgg gccggcaaga
    15481 ccttcgcctc gcagtacttc ggcaccgtgc ccttcggcat gagcttcaac ggccagcatg
    15541 cctggctgca gttcggcggc ggcaacgatt tgtggcgcga gatctacaag cccttcaagg
    15601 tcctgccttt cgccatcggc tgctcgggcg tgcagatgac cggctggttc aagcgcgagg
    15661 tcaaaagcgt cgacgatttc aagggattga agatgcgcat cccgggtctg gccggcaggg
    15721 cctatgcgga actgggcgtg gacgtgaagc tgctgcccgg cggcgagatc ttccccgcgc
    15781 tggaacgcgg cgtgatcgat gcggcggaat gggtaggtcc ctatctcgat caccgtctgg
    15841 gtctgcagaa cgcggcgaag tactactaca ccacgggttg gcacgagcct tcgacgacct
    15901 cggaactggt agtcaacgaa gccgcgtaca acaagctgcc gaaggatctc caggcgatcg
    15961 tcaaggcggc cgcggacgcc accaaccagg aaggcctcct gctgttggag gcccgaaacg
    16021 cggcggcgct ggacgaactg gtgaacaagt acggcgtgaa gatctcccgt ctgccggagg
    16081 acgtcatcaa gcggctgcgc gaggtctcga tggacatctt ctcggaagcg gccgccaagg
    16141 acgcgatggt gaagaaggtc cacgattcgt acttcgcctt caagaagacg cacgatcact
    16201 ggaaccagat cagcgaaacg acattccaga cgactgtccg cggactcggt taaggaggaa
    16261 cgcggcgggc ggctcagccg cccgccgctg tccgtgcgcg tttatgcggc ggcgtgaggg
    16321 cgccgcccaa ctgcctggga tgcacgcatg aaaaacaaac ttataaaaac ggccgacggt
    16381 atcgatcggt tcatcgactg gacggggctg ttcgccgcct ggctctgtct ggtgctggtc
    16441 ctgctcatcg cggtggaagt gatcctgcgt tacaccatga gtctcggtgc ggtgtgggcg
    16501 caggagttgg agtggcacct ccttgccgtc atcgcgctgt gggggatctc ctacacgcag
    16561 aaggaggacg cgcacgtccg tgtcgacatc ctctatcaga acttcagtca acgcaccaag
    16621 gactggctcg agttcttcag cacactgctg gtaatggtgc ccttgtcgtt ctacgtcgcc
    16681 tggctggcct gggatttcat ggcgcaggcc taccgcatcg gcgagatctc gcccgacccg
    16741 ggcggcctga cgcaccggtg ggtgctcaag tccttcgtga tgaccgggtt catcctgctc
    16801 ggcatccaaa gcactgcgct tatgctgcgt agcgcggcgc gcgtcttctt caataacaac
    16861 aacgataacg gaggcgagca tgccgcttaa cgagatctac gccatcggga tgctgctgag
    16921 cttcgtcgcg ctgctgcttg ccggcgtgcg cgtggcttat gccattgcgg catcgggctt
    16981 cattttcggc ctgatcggct tcgggccgct gctgttcaac ctgctgcccg cgcggatgta
    17041 cggcgtggcg accaactaca cgctgctcgc cgtgccgctg ttcgtgttca tgggcgtgat
    17101 gctggagaag tcgcacatcg cggaggacat gctcgacgtg atgggtcacc tcgccggccg
    17161 ggtgaacggc ggcatgggcg tggccatcat tctcgttggc atgatgatgg gcgcggccac
    17221 cggcatcgtc ggcgcgacgg tcgtaacgct cggactgatc gcactgccga cgcttttgcg
    17281 ccgcggctat aacacggggc tggcgaccgg ttcgatctgc gcatccggca cgctggggca
    17341 gatcattcca ccgagcctga ttctgatcct gctgtcggac ctgatgggcg agtcggtcgg
    17401 cacgctgttc gccgccgcgc tcatgccggg cctcatgctc gcggccctgt atgtgctcta
    17461 catccttggg ttgggcctga tccgtcccga tcttgcgccc gcactgccca aggaagaacg
    17521 ttcccgcgta accggtcgcg aactgttcaa gcgcacattg ctcggcgtcg tgccgcccat
    17581 cgcgctggtc ttcgcggtgt tgggttcgat catcggcggc attgctgcgc cgacagaggc
    17641 ggcggccatg ggtgcggcgg gttcgatcct ggtcgtcgcc gcgcttggca agctgaacaa
    17701 caaggtgatg agcgagacca tgcgcgacac cttgaagacc acggcgatga tcttcttcat
    17761 cgtcatggct tcgcaggtgt tcagcctcgc gttccgcggc ctttcgggcg aagacctcat
    17821 tgccgagttc atgggtgcaa tccccggcgg aatctggggc gcgatcatct tcatgatgtt
    17881 gctgctgttc attctcggct tcttcctcga gtggatcgag atctcctaca tcgtgctgcc
    17941 gttgctgctg ccgatcttcg tcgcggcgga cgtcaacatg gtgtggctgg cgatgctgat
    18001 ctgcatgaac ttgcagacct ccttcctcac gccgccgttc ggctgggcgc tgttcttcat
    18061 gaagggctgc gcgccgccgg gggtcaagac gggcgacatc tacaagggtg tcatcccgtt
    18121 catcggcatc cagatcgtcg ggctgacgct gctcgcgctg ttcccgcaga tcgcgctgtg
    18181 gctgccgact gccatcggct ggatgaagtg atggtgtgaa cgacggtgag cggcggccgc
    18241 ttcggcggcc gccgcccgaa gggaggaaca tgcagaacac cgcctgtggc gtgcgcggca
    18301 ttgccgtcgc accgcatgca ctcgcggcgc agtcggcgat cgcggtgctg cgcgagggcg
    18361 gcaacgccct ggaagcgatg atcgcggcgg cggcgaccat tgccgtcgtc tatccccata
    18421 tgaacggcat cggcggcgat gccttctggt tgttgtctgc gccgggcgca ccggtgcgcg
    18481 ccatcgatgc cagcggcgcg gcggggcgca gtgtctcgcg cgcgctctat gccgatcacg
    18541 gactcgatac cattcccatg cgcgggccgc ttgcgtccaa cacggtggcg ggcaccatat
    18601 cgggatggaa caaggcgctc gagatcggtg cggagtgggg cgggcgtctg ccgctttcgc
    18661 gtctgctcgg cgacgcgatc cactacgcgc gagacggcat tcccgtgacc gccagtcagg
    18721 cggcgagcac gcaggcgaag cttgcggaac tcgcgacgca gccgggcttc gcggaaacct
    18781 tcctgatcga cggcgcatcg ccgcaggcgg gcagtgtttt tctgcagccg cgcttggcgg
    18841 aggtgctcga acaactcgtg cgtgcgggac tggacgatta ctaccgcggc gacctggcgc
    18901 gccgtatcgc cggcgacctc gccacgatcg gcagtccgct cacgctggaa gatctcaacg
    18961 cgcatcgcgc gctcgaagtc cggccgctgg cgctgcgtca ctcgctcggc acggtgtaca
    19021 acatgacacc gccgacccag ggggtgattt cgctggcgat cctcggcgtg gcggaccggc
    19081 tcggtctgga caagctcgat c
//
LOCUS       DATGOU010000067         9442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_4174517, whole genome shotgun sequence.
ACCESSION   DATGOU010000067 DATGOU010000000
VERSION     DATGOU010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9442
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4174517"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VKZ48_03760"
     CDS             complement(<1..764)
                     /locus_tag="VKZ48_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HLU77006.1"
                     /translation="MNAALPGYAELHCLSNFSFLRGASHPEELVQQAHTQGYAALALT
                     DECSLAGVVRAHVAAREFGLKLIIGTEVRLASGIKLVLLAQDRVGYGHLSTLITRARR
                     RSTKGRYRLVMEDMEAGLPHCLALLIPRHDASDDAAARWLARRFSGRAWIAVELLRGP
                     NDRAALAALRALSRRTGVPLVAAGDVHMHVRSRQPLQDTLTAIRLGKKVDECGHALYP
                     NAERHLRARVQLAQIYPPALLAETLHIVERCSFSLD"
     gene            complement(761..2014)
                     /locus_tag="VKZ48_03765"
     CDS             complement(761..2014)
                     /locus_tag="VKZ48_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HLU77007.1"
                     /translation="MLWIALYFPCLVEAADDSELQQLAAWAGRYTPNVSPEKTCGLLL
                     EVAGSLKFYGGLPALVRSMRADLKTMGYHAALAGAPTARAAWWLARAERSRFIATLPP
                     LPEALAPLPLAVLDSEAKTLNLLRRSGLRTLGEVMGLPRDSLAKRCGQALLDQFDRAT
                     GRLPEVRPYFQPPPVFHARQALQTEVCHAEPLLHVARRLLLQLGGYLLARNAGIEGFD
                     FVLQHRDGPCTTIDIGLVAPSRDSAHLLHLLRERLERVVLREPVREVRLHAEHIHALA
                     DDNASLFHDEISASQDWPRLVEQLRARLGDDSVRGLALAATHRPEDASVLTDIGSAPR
                     VDTRFGLRPLWLLSAPKALVERQGVPHYDGPLKLLIGPERIQSSWWSGPYEGRDYFVA
                     QTPAHALLWVYRVAARGWYLHGWFA"
     gene            complement(2034..2699)
                     /gene="imuA"
                     /locus_tag="VKZ48_03770"
     CDS             complement(2034..2699)
                     /gene="imuA"
                     /locus_tag="VKZ48_03770"
                     /inference="COORDINATES: protein motif:HMM:NF033429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HLU77008.1"
                     /translation="MLTDLLVRSDIRRATDLARVARPSVPTGFPALDAQLPGGGWPSG
                     ALTEILPAHQGIGELRLLGPALAGLSRKGRRLAWIAPPHLPYAPALDAADIDLSTLLI
                     VRTTSLTDTLWAAEQCLRSAACGAVLLWPQQLKYAELRRLQLAADGTDAMAVLFRPPS
                     AADQASPAALRLGLATADGGLAVRILKRRGAALQSPVRVEIGPPTARTPFSAPLPDTT
                     TPA"
     gene            complement(2756..3358)
                     /gene="lexA"
                     /locus_tag="VKZ48_03775"
     CDS             complement(2756..3358)
                     /gene="lexA"
                     /locus_tag="VKZ48_03775"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HLU77009.1"
                     /translation="MKELTPRQAEILQLIRDSIEETGFPPTRAEIARILGFASANAAE
                     EHLKALARKGAIELMEGTARGIRLAQPLGLPLVGRVAAGSPILAEEHIQSRYQIDPSL
                     FKPRADYLLKVRGMSMRDAGILDGDLLAVHRTEDVRSGQIVVARLQDEVTVKRLKRRG
                     ATVELLAENPDFEPIVVDTRNEAFAIEGLAVGVIRNSRMM"
     gene            complement(3373..3771)
                     /locus_tag="VKZ48_03780"
     CDS             complement(3373..3771)
                     /locus_tag="VKZ48_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77010.1"
                     /translation="MDQDKARALLQALAAGIDPHSGENFPSDSPYQHPDVVRALYAAL
                     EALNTRGVKTAATTPRSAPGNAGKPWTKEEDERLLAAFAGGMSLEALAAQHGRSRTGI
                     EARLARHGKVPPPANLPSSRLKAREASPAF"
     gene            complement(3844..3993)
                     /locus_tag="VKZ48_03785"
     CDS             complement(3844..3993)
                     /locus_tag="VKZ48_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HLU77011.1"
                     /translation="MSLTTILLIVLVLLLIGALPVYPYSTGWGYAPSGILVAVLVIVV
                     LFVLI"
     gene            4154..4357
                     /locus_tag="VKZ48_03790"
     CDS             4154..4357
                     /locus_tag="VKZ48_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HLU77012.1"
                     /translation="MFPKKIKTGYESDTTRFINDLLEKKPEILEERQRGRSMWWDKKL
                     DLEKLKRDQESHVKPSPYVYQNY"
     gene            4365..5042
                     /locus_tag="VKZ48_03795"
     CDS             4365..5042
                     /locus_tag="VKZ48_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLU77013.1"
                     /translation="MRRTLLAAALSLLLAGCLSPAQSLMLALIPDGTFSTLLSNMQGV
                     QDVNRQRLSELAAQEDWAGVVQFAEKNLAVDPTIADWWIVAGYAHTRLAQYERANRAF
                     AEAVRLEPDEMNAWHLLAESYRAMGQPGRALRTLDNAMRVSRDDPNTYFLLGQNFDDL
                     KRIDRAVPHYEQAVQRNPQFVEAWYALGRDYLRLGRRADYENAVAALRELNPRAADHL
                     ATLGAEK"
     gene            complement(5050..5751)
                     /locus_tag="VKZ48_03800"
     CDS             complement(5050..5751)
                     /locus_tag="VKZ48_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLU77014.1"
                     /translation="MNRDSIERVYSTYARIYDHLFGRVFQEGREAAMARMAPQAGERI
                     LEVGVGTGLLLPLYPSHCEIVGIDLSDKMLDEARKRAASLQLENVELHHMDASDMEFE
                     DDSFDTVVAAYVITAVPDYRAVMREMIRVCRPGGRILLLNHFTNGNRLLAACEKAVSP
                     LCTHLGFRTDLSVREVLSGWPVDVQADAKIKPLGMWHLVECRNAKHEKAHGTMHSQAA
                     PYAGAAAYSIPRSRQ"
     gene            complement(5909..9427)
                     /locus_tag="VKZ48_03805"
     CDS             complement(5909..9427)
                     /locus_tag="VKZ48_03805"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLU77015.1"
                     /translation="MLRLAHNLQFADLYQREGLLKLDTHFLDFVGTAAPPLKERLIAA
                     RANPDALTQKDESTLLLDLAPHLERFLTQLFGITDAVLALTAKHEALAPLYSVKRQFV
                     QRRAATRIKPAEAAEIDGPDIARELTELFGNRFDELIFATHVEVWLKDEEAHARPLEL
                     ALKYAAWAVHTPAGRHAHRDGVLFKLPAKTDPYNLMLHVRRHEEDGVTSFRIDPEHLR
                     RREGFGLTDPGTDLAGALDQANYCIWCHNQGKDSCSKGLKEKAPAEAPQQVTYKKSVF
                     NITLAGCPLEEKISEFHVAKTHGLPLAALAIITVDNPMVAATGHRICNDCMKACIYQK
                     QQPVDIPQSETRTLKDVLELPYGFEIYSLLTRWNPLNIRRPYPAAPTGYKVLVAGMGP
                     AGYSVSHNLINDGHTVVGVDGLKIEPLDPALNGVAADGTRVPFEPVRDVNELFEALDE
                     RISAGFGGVVEYGITVRWNKNFLKIIRLLLERRAGFAMYGGVRFGGTITLEDAYARGF
                     DHVVLAMGAGKPTVLDIPNGLARGVRTASDFLMALQLTGAAKFDSIANMQVRMPVVVI
                     GGGLTAIDTATEALAYYPVQVEKFLRRYEDLAKDIGEDALRMQWDAEESEIAEEFLTH
                     ARAIRAERARAEKEKREPRILELLQQWGGAIIAYRRRLIDSPSYTLNSEEVEKALEEG
                     AVFAECLNPIAIDVDEYGAAKSVRFTRQQRDENGKWSAVGEHTLPARSVFIAAGTQPN
                     TVLAREDAAHFKLDGKYFQACDEEGNPVKPQYSISKPSSPDVLLSRQPDGRFVSYFGD
                     LHPSFFGNVVKALGSAKQGFPVVSRVLAQRPPADPRKPAEFFAELSNDLLATVHEVKR
                     LTPTIVEVVLKAPLAARKFEPGQFYRLQNFESLAPLALDTRMAMEGIALTGASVDREK
                     GLVSTIALEMGGSSNLCAALQPGEPVILMGPTGTPTEIQANETVVLVGGGLGNAVLFS
                     IGQAFREKGSKVLYFAGYKKMIDRYKIEEIEAAADVVVWCSDEAPGFTPNRPQDYAFV
                     GNIVQAMEAYASGKFGDTPIDMRQVDRIIAIGSDRMMAAVGQARHESLKPYLKPHHYA
                     IGSINSPMQCMMKEICAQCLQPHRDPETGEVRYVFSCFNQDQPLDAVDFQGLADRLKQ
                     NSLQEKLTAQWLAHCMEDLRRKRPMV"
BASE COUNT         1651 a         2908 c         3169 g         1714 t
ORIGIN      
        1 tcgtcgagcg agaagctgca gcgctcgacg atgtgcaggg tttcggcgag cagcgccgga
       61 gggtagatct gtgccaactg cacgcgcgcc cgcagatgcc gctcggcgtt gggataaagg
      121 gcgtggccgc attcatccac cttcttgccc aggcggatgg cggtcagcgt gtcctgcagg
      181 ggttggcgcg agcgcacgtg catgtgcacg tcgcctgctg cgaccagcgg cacgccggtt
      241 ctgcgcgaaa gcgcccgcag tgcggccagt gcggcgcggt cgttcgggcc gcgcagcagt
      301 tcgacggcga tccaggcgcg gccggaaaaa cggcgggcaa gccagcgtgc cgcggcatcg
      361 tcgctcgcat catgacgggg aatgagcagc gcgaggcaat gcggcagacc tgcttccata
      421 tcctccatga caagacggta acgccctttg gtcgaccgcc gtcgggctcg ggtgatgagc
      481 gtcgacaagt ggccgtagcc cacgcgatcc tgtgcaagca gtacgagctt gatgcctgaa
      541 gcgagccgca cttcggtgcc gataatgagc ttcaggccga attccctggc cgccacatgc
      601 gcgcgcacca cacccgccag ggagcattcg tcggtgagcg cgagcgccgc atagccttgc
      661 gtgtgtgcct gctgcaccag ttcctcggga tgcgatgcgc cgcgcaggaa actgaagttg
      721 gacaggcagt gcaactccgc atagccgggc agcgcggcat tcatgcgaac cacccgtgca
      781 gataccagcc gcgcgcggcc acgcgataaa cccaaagcag cgcatgggcg ggggtctggg
      841 cgacgaaata gtcgcgccct tcataagggc cgctccacca actcgattga atgcgttccg
      901 gaccgatgag cagtttgagc ggcccgtcgt aatgcggcac gccttgccgt tccactagcg
      961 ccttcggcgc ggagagcagc cagagcggtc gcaggccgaa gcgggtatcg acgcgcggtg
     1021 cgctgccgat gtcggtcaat acgctcgcat cttccggacg gtgcgttgca gcgagcgcaa
     1081 gcccgcgcac gctgtcatca ccgaggcgtg cgcgcagttg ttccaccagg cggggccagt
     1141 cctggctcgc gctgatttca tcgtggaaga gggaggcgtt gtcgtctgcc aaagcatgta
     1201 tgtgctcggc atgcagccgc acttcgcgta cgggttcacg taagaccacg cgctccagtc
     1261 gttcgcgcaa tagatgcaac agatgcgcgc tgtcgcggct gggtgccacc aggccgatgt
     1321 cgatcgtcgt gcaaggacca tcacggtgtt gcagtacaaa atcgaagccc tctatgccgg
     1381 cgttgcgggc gaggagataa ccgcccagtt gcaacagcag gcgtcgcgcg acatgaagca
     1441 acggctcggc atgacagact tcggtctgca acgcctgccg agcgtgaaaa acgggtggtg
     1501 gctgaaaata aggacgcacc tcgggcagcc gtcccgtcgc gcggtcgaat tgatcgagca
     1561 gcgcctgacc gcaacgcttg gcgagactat cgcgcggcag tcccatcact tcacccaatg
     1621 tccgcaatcc gctacgccgc aataagttga gggttttggc ttcgctatcg agaacggcga
     1681 gcggcagggg tgcgagcgct tcgggcagcg gcggcagggt cgcgatgaag cgcgaacgct
     1741 ccgctcgcgc aagccaccat gccgcgcgcg cggtcggtgc gccggcgagg gcagcgtgat
     1801 aacccatggt tttcaaatcg gcacgcatgc tgcggaccaa agctggcagg ccgccgtaga
     1861 acttcaggct gcccgccact tcgagtagca ggccgcaggt tttttccggc gacacattcg
     1921 gcgtgtagcg cccggcccag gcggccagct gctgcagctc gctgtcgtcg gctgcctcga
     1981 ccagacaggg gaaatagagg gcgatccaca acattgttgc agcctgggtg ggcttaggcc
     2041 ggtgtcgtgg tgtccggcag cggagcagag aacggggttc tggcagtggg cggcccgatc
     2101 tctaccctga ccgggctctg gagcgcggcg ccgcgccgct tgaggatgcg cacggccagg
     2161 ccgccatcgg cggtggccag gccgagacgc agcgctgcgg gcgaagcctg atcggctgcg
     2221 gagggcggac gaaaaagcac tgccatcgcg tccgtgccgt ccgccgccaa ttgcaggcgt
     2281 cgcagctcgg cgtacttgag ttgctgtggc cacagcagta ccgctccgca cgcggcgctg
     2341 cgcaggcact gttcggccgc ccagagcgtg tcggtgaggc tggtcgtgcg tacgatcaat
     2401 agcgtgctca agtcgatgtc cgccgcatcc agtgccgggg cgtagggcag gtgcggcggt
     2461 gcgatccagg ccagtcgccg gcccttgcgc gacagaccgg ccagcgccgg gcccagcagg
     2521 cgcaattcgc cgatgccctg gtgcgcgggg aggatttcgg tcagcgcacc gctgggccag
     2581 ccgccgccgg gcagttgcgc atccagtgcg gggaaaccgg tgggcacgct cggtcgggcg
     2641 acgcgcgcca gatcggtggc tcgccggata tccgaacgga ccaggaggtc ggtgagcaca
     2701 ggtgcgcgag gcaacatgac gaacctgaag tgacgcggcg cgaaccgcgc cgctcttaca
     2761 tcatgcgact gttgcggatg acgccgactg cgagcccttc gatggcaaag gcttcgttgc
     2821 gagtatcgac cacgatcggc tcgaaatccg ggttctcggc cagcagttcc accgtcgcgc
     2881 cccggcgctt gaggcgtttg actgtgacct cgtcctgcag acgggcgacg acgatctgtc
     2941 ccgagcgcac gtcttcggtg cgatgcacgg ccagcaggtc gccgtccaga atgccggcgt
     3001 cgcgcatgct cataccgcgg accttgagca gatagtcggc gcgcggtttg aacagggaag
     3061 gatcgatctg ataacggctc tggatatgct cctcggccag gatgggcgag ccggcggcga
     3121 cacggccgac cagcgggagg ccgagcggct gcgccagacg gataccgcgc gcggtgcctt
     3181 ccatgagttc gatggcgccc ttgcgcgcca gcgccttgag atgttcctcg gcggcattgg
     3241 ccgaggcgaa ccccaggatg cgggcgattt ccgcgcgcgt gggggggaaa ccggtctcct
     3301 cgatggagtc gcggatcaat tgcaggatct cggcctgccg gggtgtgagt tctttcatcg
     3361 ggctcgtgtt ctttagaaag cgggggaagc ttcgcgcgcc ttgaggcggc tggaaggcag
     3421 attggcgggc ggcggcactt tgccgtgcct ggccagccgc gcctcgatgc ccgtgcggct
     3481 gcggccgtgt tgcgccgcca gcgcctcgag gctcatgccg ccggcaaagg cggccagcag
     3541 gcgctcgtct tcctctttgg tccagggttt gccggcattg ccaggtgcgg agcggggcgt
     3601 ggtcgcggcg gttttgacgc cgcgcgtatt caaggcttcc agcgcggcat acagcgcccg
     3661 aacgacatcc ggatgctggt aggggctgtc cgaggggaag ttttcgccgc tgtgcgggtc
     3721 gatgccggcg gcaagggctt gcaacagggc acgtgccttg tcctgatcca tggagcctcc
     3781 tcgttcgcta agccgggcgt tgatggctgt agttatatac agtcctggat ggaaaggcaa
     3841 gcctcagatc agcacgaaca gcaccacgat caccagtacc gcgacgagga tgccgctggg
     3901 cgcatagccc cagccggtgc tatagggata gaccggcagt gcgccgatca acagcaggac
     3961 gagcacaatc agcagaatag tcgtcagaga catgggcgga ccttctgaaa cggctcttcg
     4021 aggcaccgga acccgctggc tcgaggccag caacacccgt gccgaaggag gcttgtggcg
     4081 cagctttcac acgacgtttt acgccccgcc tgcgcttata atcccgaggt catccaacac
     4141 tgcttgctgc accatgttcc ccaagaaaat caagaccggc tacgaatccg acaccacccg
     4201 attcatcaac gatctcctgg agaaaaaacc ggagatcctc gaggagcgcc aacgcggccg
     4261 ctctatgtgg tgggacaaga aactcgatct ggaaaagttg aagcgcgacc aggagtcgca
     4321 cgtcaagccg tcgccctacg tctatcagaa ctactgagag gcgcgtgcgt cgcaccctcc
     4381 tcgcggcggc gctgagtctg ttgctggccg gctgtctgtc gccggcgcag agcctgatgc
     4441 tcgcgctgat tccggacggc accttctcca cgctgctgag caacatgcag ggggtgcagg
     4501 acgtcaatcg gcagcgtttg agcgaactgg cggcgcaaga ggattgggcc ggcgtcgtgc
     4561 agttcgcgga aaagaatctg gcggtggatc ccactatcgc cgactggtgg atcgtcgccg
     4621 gctatgccca cacccggctg gcgcagtacg agcgcgccaa ccgagccttt gccgaagcgg
     4681 ttcgtctcga gccggacgaa atgaatgcct ggcacttgct ggccgagtcc tatcgcgcca
     4741 tgggacaacc ggggcgcgcc ttgcgcacgc tggataacgc catgcgggtt tcgcgggacg
     4801 accccaacac ctatttcctg ctgggacaga acttcgacga tctcaagcgg atcgatcgcg
     4861 cggtgccgca ttatgaacag gcggtccagc gcaatccgca attcgtcgaa gcctggtatg
     4921 cgctggggcg ggattatttg cggctcggcc gtcgtgccga ttacgaaaat gcggtggcgg
     4981 ccctgcggga gttgaatcca cgcgccgccg atcacctggc gacgctcggc gcagagaaat
     5041 aggcggcccc tactggcggg agcggggaat ggaataggcc gccgcgcctg catacggcgc
     5101 agcctgactg tgcatcgtgc cgtgcgcctt ttcgtgtttg gcgttgcggc attcgacgag
     5161 gtgccacatc cccagcggtt tgatcttcgc atcggcctgc acatcgaccg gccatccgct
     5221 gagcacttcc ctgaccgaaa gatcggtgcg gaatcccagg tgcgtacaga gcggcgagac
     5281 ggctttctcg cacgcggcga gcagccggtt gccgttggtg aagtgattga gcagcaggat
     5341 gcgcccgccc ggacggcaga cacggatcat ttcgcgcatc accgcacggt aatcgggcac
     5401 ggcggtaatg acgtaggcgg caacgacggt gtcgaagctg tcgtcctcga actccatgtc
     5461 cgaggcgtcc atgtggtgca gttcgacgtt ctccaactgc agcgacgcgg cgcgcttgcg
     5521 cgcttcgtcc agcattttgt cggacaaatc gatgccgacg atttcgcaat ggctgggata
     5581 gagcggcagc agcagcccgg ttccgacgcc cacttcgagt atccgttcgc cggcctgcgg
     5641 cgccattcgc gccatggccg cttcccggcc ttcctgaaaa acacgcccga aaaggtggtc
     5701 gtaaatacgg gcgtaagtgg aataaacgcg ctcgatgcta tcgcgattca caacatctcc
     5761 ccatggcttg cggacgaaat ccgccgcgat ggagcgatcc gacaggcgcc ggcgtccatc
     5821 gcaacgttga gacggcgcgg gcccgtacgc ttgtgaccgg cccgggcgaa gtggaattct
     5881 cgccgcgggc acatgccgga atctcgcttt acaccatcgg gcgcttgcgc cgcaggtcct
     5941 ccatgcaatg agcgagccat tgcgcggtga gcttctcctg cagggagttc tgcttcaggc
     6001 gatcggccag gccctggaaa tccactgcgt cgagcggctg atcctggttg aagcaggaga
     6061 acacgtagcg cacttcgccc gtttccggat cgcgatgcgg ttgcaggcac tgcgcacaga
     6121 tctccttcat catgcattgc atcggcgagt tgatcgaacc gatcgcgtag tgatgcggct
     6181 tgagataagg cttgagcgac tcgtgtcgcg cctggccgac ggcggccatc atgcgatccg
     6241 agccgatcgc gatgatgcga tcgacctgtc gcatgtcgat cggcgtatcg ccgaatttgc
     6301 cgcttgcgta ggcctccatc gcctggacga tgttgccgac gaaggcgtaa tcctgcggcc
     6361 ggttcggcgt gaagccgggt gcttcgtcgc tgcaccagac gaccacatcc gcagccgctt
     6421 cgatctcttc gatcttgtag cggtcgatca tcttcttgta gccggcgaag tagaggactt
     6481 tggacccctt ctcgcggaag gcctggccga tggaaaacag tacggcattg cccagaccgc
     6541 cgccgacgag cacgacggtt tcgttcgcct ggatttctgt cggcgtgccg gtcggcccca
     6601 tgaggatgac cggttcgccc ggttgcagcg cggcgcacag attcgatgag ccgcccattt
     6661 ccagcgcgat ggtggaaacc aggccttttt cgcgatcgac cgaagcaccg gtcagcgcaa
     6721 tgccttccat ggccatgcgc gtgtcgagcg cgagcggggc gagcgattcg aaattttgca
     6781 gacgatagaa ctgacccggc tcgaacttcc gcgcggcgag cggtgctttg agcacgactt
     6841 cgacgatggt cggcgtgagg cgcttcactt catgaaccgt ggcgagcaga tcgttgctca
     6901 actcggcgaa gaactcggcc ggcttgcgcg gatcggcggg cggccgctgc gcgagcacgc
     6961 gactcaccac cgggaagccc tgcttggccg aaccgagcgc cttcaccacg ttgccgaaga
     7021 aggacggatg caggtcgccg aagtacgaca cgaagcggcc atcgggctgg cgcgacagca
     7081 gcacatcggg gctggacggt ttggagatcg agtactgcgg cttgaccgga ttgccttcct
     7141 cgtcgcaggc ctggaagtac ttgccgtcga gtttgaaatg cgcggcgtct tcgcgcgcga
     7201 gcaccgtatt gggctgcgtg cctgcggcga tgaacaccga gcgggcgggc agggtatgtt
     7261 cgccgacggc gctccatttg ccgttctcgt cgcgctgctg cctggtgaag cggacgctct
     7321 tcgcagcgcc gtattcgtcg acatcgatgg cgatcgggtt caggcattcg gcaaacaccg
     7381 cgccttcttc gagcgccttc tccacttcct cgctgttcag cgtgtaggag ggactgtcga
     7441 tcagtctgcg gcgataggcg atgatcgcgc cgccccactg ctgcagcagt tcgaggatgc
     7501 gcggctcgcg cttctccttt tcggcccgcg cgcgttccgc gcggatggca cgtgcgtggg
     7561 tcaggaactc ctcggcgatc tcgctttctt ccgcgtccca ttgcatacgc agggcgtctt
     7621 cgccgatgtc cttcgcgagg tcctcgtagc ggcgcaggaa cttctccacc tgcaccgggt
     7681 agtaggccag cgcttcggtc gcggtgtcga tcgccgtcag tccgccgccg atcaccacta
     7741 ccggcatgcg tacctgcatg ttggcgatgg aatcgaactt cgctgcgccc gtgagctgca
     7801 gcgccatgag gaagtcggac gcggtacgca cgccgcgtgc gagaccgttg ggaatgtcca
     7861 gcaccgtggg cttgcccgcg cccatggcga gcacgacgtg atcgaagccg cgggcatagg
     7921 cgtcttcgag cgtgatggtg ccgccgaagc gcacgccgcc gtacatggcg aagccggcac
     7981 ggcgttccag caacagccgg atgatcttga ggaagttttt gttccagcgc acggtgatgc
     8041 cgtattcgac cacgccgcca aagccggccg aaatacgctc gtcgagcgct tcaaacagtt
     8101 cgttgacatc gcgcaccggt tcgaagggaa cccgagtgcc gtcggccgca acaccgttga
     8161 gcgccgggtc gagcggctcg atcttgaggc cgtccacgcc gacgacggta tggccgtcgt
     8221 tgatcaggtt gtgcgacacg gagtaacccg cggggcccat accggcaacc agcaccttgt
     8281 agccggtcgg tgcggcggga tagggacggc ggatgttgag cgggttccag cgcgtcagca
     8341 gcgaatagat ttcgaaaccg tagggcagtt cgagcacgtc cttgagcgtg cgcgtttccg
     8401 actgcggaat gtcgaccggc tgctgcttct ggtagatgca ggctttcatg cagtcgttgc
     8461 agatgcgatg accggtcgcc gccaccatcg ggttgtccac ggtgatgatg gccagtgccg
     8521 cgagcggcag gccgtgcgtc ttcgcgacat ggaattcgga aatcttttct tccagcgggc
     8581 agcctgcgag cgtgatgttg aagacgctct tcttgtaggt gacctgttgc ggtgcttcgg
     8641 cgggcgcttt ttccttcaag cccttcgagc aggagtcctt gccctggttg tgacaccaga
     8701 tgcagtagtt cgcctggtcg agcgcgccgg ccagatcggt gcccggatcg gtgagaccga
     8761 agccttcgcg tcgccgcaga tgctcagggt cgatgcggaa gcttgtgacg ccgtcctctt
     8821 cgtgacggcg cacatggagc atcaggttgt agggatcggt cttggcaggc agcttgaaca
     8881 gcacgccgtc gcggtgcgcg tgacggccgg ccggcgtatg caccgcccag gcggcgtact
     8941 tcagcgccag ttcgagtggc cttgcgtgtg cttcttcgtc cttcagccat acctcgacat
     9001 gcgtggcgaa aatcagttcg tcgaagcggt tgccgaacaa ctcggtcagt tcgcgcgcga
     9061 tgtcgggtcc gtcgatctcg gccgcttccg ccggcttgat ccgggtagcg gcacgccgtt
     9121 ggacgaactg gcgcttgaca ctgtagagcg gcgccagcgc ttcatgcttc gccgtcaggg
     9181 caagcaccgc gtccgtaatg ccgaacaact gtgtgagaaa acgttccaga tgcggtgcga
     9241 gatcgagcag cagcgtggat tcatccttct gcgtcaacgc gtcggggttg gcgcgcgcgg
     9301 cgatcaggcg ttctttcagc gggggtgcgg cggtgccgac gaaatcgaga aagtgcgtgt
     9361 cgagcttcag gagcccttca cgttggtaga ggtcggcgaa ctggagattg tgtgcgagtc
     9421 gaagcattcg gcggtcggcg cg
//
LOCUS       DATGOU010000068        15243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_4174668, whole genome shotgun sequence.
ACCESSION   DATGOU010000068 DATGOU010000000
VERSION     DATGOU010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15243
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4174668"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /gene="flhC"
                     /locus_tag="VKZ48_03810"
     CDS             complement(<1..474)
                     /gene="flhC"
                     /locus_tag="VKZ48_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HLU77016.1"
                     /translation="MAKSVIAEARDIKLATELIELGARLQVLESETRLSREKLLRLYK
                     EVQGKSPPKGMLPFSTDWFMTWQPNIHSSLFMSIYSYIDKSVELEQIEVLVKAYRLYR
                     EHVAAHGMEEVLSVTRAWRLVKFFDAGMLTTTSCSCCSGQFVVHTYELTDTYVCGL"
     gene            complement(504..827)
                     /gene="flhD"
                     /locus_tag="VKZ48_03815"
     CDS             complement(504..827)
                     /gene="flhD"
                     /locus_tag="VKZ48_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HLU77017.1"
                     /translation="MKQDQLLDEIRDANLSYLLLAQRMIREDRAQAMFRLGVNEQVAD
                     IIGDLSMAQLIKIASSNMLMCSFRFGEDMVWNLLTGHSKDADIARMHASLLMANAPAA
                     EAVPA"
     gene            1406..2848
                     /locus_tag="VKZ48_03820"
     CDS             1406..2848
                     /locus_tag="VKZ48_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HLU77018.1"
                     /translation="MPQIINTNIASLNAQRNLNTSQTALATSLQRLSSGLRINSAKDD
                     AAGLAISERFTTQIRGLTQAARNASDGISLAQTGEGALAELGANLQRIRELAVQAANS
                     TNSASDRAALDLEVQQRLEEINRIANQTSFNGQKILDGTFGTAAFQVGANVGETITIN
                     LNTSMRGTDIGAVAQATSSGIAALTTSTSSASGAFTDAVSNADGQRFTLTVGNVVLLD
                     VEQDGAPVTVNDTMLDAALTAKADELAEAGFTVTGTFGTDNVVFSRVDGADFDITVDS
                     TFNTTGGFAGADWPGTVSATGVDLDAGDLTIALGSAEAVDIKGEFSTAQGLADAINTA
                     VAGVNATVTEAGEIQLTSTGQNIVIGGTEAATLGLTSATASGNLSGVNVKDVDAANDA
                     IQRIDSALTSVSDLRSTLGAIQNRFESTIANLQATTENLSASRSRILDADFAAETAHL
                     TRGQILQQAGVAMLAQANSLPNNVLALLRG"
     gene            2981..3310
                     /locus_tag="VKZ48_03825"
     CDS             2981..3310
                     /locus_tag="VKZ48_03825"
                     /inference="COORDINATES: protein motif:HMM:NF015602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HLU77019.1"
                     /translation="MQIDPLSIATTGITVPAAATRPAPAPVAAVPQSPPSDAELQAAV
                     AKINQVLKESRNSLQFEIDGETGKTIVRVIDEDTKEVLRQFPSEEVLAIARAIPPISG
                     ALLNDTA"
     gene            3340..5298
                     /gene="fliD"
                     /locus_tag="VKZ48_03830"
     CDS             3340..5298
                     /gene="fliD"
                     /locus_tag="VKZ48_03830"
                     /inference="COORDINATES: protein motif:HMM:NF018852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HLU77020.1"
                     /translation="MALSAPGIGSSLDVNSIVQQLMAVEQRPLQALAQKEASYQAKLS
                     AYGSIKSGVSAFKSAMEALTSTDKFISSRVTVSNKDALGVTANGQAVPGRYNIDIQAL
                     AQSQKLSSGNYAATTDTVGTGKLTIQFGSYSEADGSFTANADKAAKTIEIEPGNATLA
                     GVRDAINAADAGVTATIINDGVGYRLVLAAKDTGLANAMRISVSDDGGSGLAALAYDA
                     SGDGPSNLTETVQARNAELTIDGIAISKASNTISDAIDGVTLNLLDQAEKTISVSITR
                     DNSGTKTAVDNFVKAYNDLYGMLSNMSAYNAETKQAGVLQGDSTIRSMQTSLRTMMST
                     ALDSAGGGLSRMSDIGVTIQVDGKLKVDSAKLQKAIDDPSKDIATLFASIAKPSDSLV
                     SFVSAGADAKAGTHALEITQLATQGRAEGSQAAATAITAGVNDKLMLEIDGVKATITL
                     NAGTYTATSLAAELQSKINGAAELRDNDVSVEVTESGGVLTITSERYGSASKVQITGG
                     NARTGLFGSVTSTDGVDVAGTMGGQAATGSGRKLTGLGISLDITGGALGPRGTISFAQ
                     GFAERFVNLAERFLADDGVLDARQDGLNRSIKDIADRRDAFNLRLETIEKRYRAQFTA
                     LDVMIASMQQTSSYLQQQLASLPKIGGE"
     gene            5373..5783
                     /gene="fliS"
                     /locus_tag="VKZ48_03835"
     CDS             5373..5783
                     /gene="fliS"
                     /locus_tag="VKZ48_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HLU77021.1"
                     /translation="MFGSFGTNAYAQVEVETGVATADPHKLVGMLYEGLLQSIARGRE
                     AMLAKEIARKGESLSKAVRILEEGLRGSLDRERGGEIAQQLDSLYEYMITRLLAANLR
                     NDLEALDEVTRLVAELRDAWQAMPQEARTSMAAA"
     gene            5827..6150
                     /locus_tag="VKZ48_03840"
     CDS             5827..6150
                     /locus_tag="VKZ48_03840"
                     /inference="COORDINATES: protein motif:HMM:NF017237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HLU77022.1"
                     /translation="MGVETGALDFYESIADASEQMLSAARRADWDALIEAEKECARRV
                     AALRAAGITGSLGREADRRRHDILRTVLAHDAEIRALTQPWMVKLEVLLNSAAAARRV
                     EQAYR"
     gene            6224..6451
                     /locus_tag="VKZ48_03845"
     CDS             6224..6451
                     /locus_tag="VKZ48_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77023.1"
                     /translation="MFELDRIPIAPTTPTTPVSPSTPAAPVRPVVRREEDERDEHAPR
                     REEHRRPPPPPASPDGLPRIDEYAHGPYGNA"
     gene            6460..7461
                     /locus_tag="VKZ48_03850"
     CDS             6460..7461
                     /locus_tag="VKZ48_03850"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HLU77024.1"
                     /translation="MAKLGDALTALVRQVRFDKLGPLLDRGASGDGAKPAATAATPAP
                     VDPVGAKQANAGATSDALHTNVRTHDVPPGPTSPSPAPAPASVQTRLSVDAARITALL
                     GETPAAAPKQPLPVAALDVATQSPEQVARALVRSLATSGLFYESHLQEWVEGQRPLDA
                     LRAEPQQRFVATLMRAAGAPPAGNAEASAPASGGTSVPASATAATNAATAPLPEALAA
                     IVREQLDALDFRRVHWQGEVWPQQPAELEIAEEDARRRDGEAAPSQRGWATTLRLRLP
                     KLGDVEARILLDGDSFAVNVRADAKSLPALQSGRTAFAAALNEAGIRLRTVELSGHE"
     gene            7454..7804
                     /locus_tag="VKZ48_03855"
     CDS             7454..7804
                     /locus_tag="VKZ48_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006385221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HLU77025.1"
                     /translation="MNEYEREQERPLDESNAPLQSAVALAYREGDAAPKVVAKGRGLL
                     AEEIMRRAQEAGVYVHQSRELVGLLMQVDLDRHIPPQLYRVIAELLAWLYRAEQAGVP
                     PSAMPPMPGPSQGV"
     gene            7816..8559
                     /locus_tag="VKZ48_03860"
     CDS             7816..8559
                     /locus_tag="VKZ48_03860"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HLU77026.1"
                     /translation="MPYLFQQRLNSELYARYRIDSQAEIIALLRSLQEERSQVVLHFS
                     DHAYVVSRVLEVWPQQGRIALDFGPDETVNEALLAAGSAVAETNLHQVSIQFELGDFR
                     RVMLHDGPALEAALPACMLRLQRRETFRVPTPVLEPITLFVPEQERCPLNLHLRVVDI
                     SAGGAGTVCDATQFQPEAGMVFRDCQMLLPEVGLLVADMEVRHVMPLHEGNTDQLQCG
                     MRFLALPPPMASFVQRFVMQLEREWRKLR"
     gene            complement(8690..9124)
                     /locus_tag="VKZ48_03865"
     CDS             complement(8690..9124)
                     /locus_tag="VKZ48_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003800002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtoQ family protein"
                     /protein_id="HLU77027.1"
                     /translation="MSFTVYLSGEIHTDWREQIKEGAKGLDVVFTAPVTDHEASDAAG
                     DCLGVETNSFWRDHKSSKVNAIRTKTLIEKADLVVVRFGDKYKQWNAAFDAGYCAALG
                     KPYITLHAEDIIHPLKEVDAAALAWATTTDQVVEILKYVLRS"
     gene            complement(9440..10315)
                     /locus_tag="VKZ48_03870"
     CDS             complement(9440..10315)
                     /locus_tag="VKZ48_03870"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HLU77028.1"
                     /translation="MRTAAMNDTAQMIEGLDGEVDRLVKQLEIPPCPSILTRIVREMH
                     SDDPDLNKVAQWVAADVGLAAAMLKTVNSPLYGLRNKATSVQQALILLGLRNVAQLIT
                     GLLLRQAFPVAQSAAMEHFWSNSAGVAAVCYVVARELKVCDSNQAYTFGLFRDAGMPA
                     MLNKKKASYEDILTGEAFDGTAPITAVELARYGLTHAQVGAYFGTSWHLDDNVWKAIL
                     LHHDYDKWPGRAREIAVRVAAMGLLSEEIYHRYSRNHSCPEWSLAAQTVFTVLGIDDG
                     AVDDLRAEASRAFDT"
     gene            complement(10447..10755)
                     /gene="fliE"
                     /locus_tag="VKZ48_03875"
     CDS             complement(10447..10755)
                     /gene="fliE"
                     /locus_tag="VKZ48_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HLU77029.1"
                     /translation="MADMKITGIESSLNKIAMQPAQGAGGAAKADFGKAFKAALDDIN
                     ATQQNADALANAYAMNDPKVGLEETMIASSKANISFQALVQVRNKLVSAYHDIMNMQV
                     "
     gene            10919..12586
                     /gene="fliF"
                     /locus_tag="VKZ48_03880"
     CDS             10919..12586
                     /gene="fliF"
                     /locus_tag="VKZ48_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010810687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HLU77030.1"
                     /translation="MADTGTLVAQHQPPQLGGSFIYGVRAMPPQRLFTLLTGFALIIA
                     VLVGIFLWSRTPDYRVLYANLSDRDGGAVVQALQQMKVPYKFSEGGGIIYVPSDKVHD
                     VRLRLASQGLPRGGAVGFELLENQKFGLTQFQEQVNYQRALEGELSRSVQSLSAVQSA
                     RVHLAIPKPSVFLREQQKPSASVLLNLYAGRTLSREQIAGITHLVSSSVPELPTTSVS
                     VVDQNGNLLSSDGNPRMQGELDEQQLAYRHQVEASYIQRITDILEPVVGRDNLRVQVT
                     ADLDFSQTEATAEIYKPNQNPAEAAIRSQQTQESVNGSQTAGGVPGALTNQPAPAATA
                     PITDAPEEGASANTTQANLNTHRESVTNYEIDKTVRHTRTPTGTINRLSAAVVVNYRR
                     ALEPAPASAPGQAAAKPVEKLEALSAEELAKIEALVKEAMGYTAARGDSLNVANVPFT
                     VPEAAPAPVEPPVWKQPQYIELAKDVGKNLLIGALVLIVLFGFVRPLIRTARNSMTVA
                     PAPAQLTAAPQAAAASYEERLQSTRELARSDPKMVASVVRNWVAQDD"
     gene            12579..13574
                     /gene="fliG"
                     /locus_tag="VKZ48_03885"
     CDS             12579..13574
                     /gene="fliG"
                     /locus_tag="VKZ48_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HLU77031.1"
                     /translation="MTEDGLNRAAILLLSLGEQEAAEVFKFLGPKEVQKLGAAMAKLK
                     NVKREKIEEVLEAFHADTNVQTSLGMDSNEYIRSVLTRALGEERATHLIDRILQGGDT
                     SGIEGLKWMEAGMVAELIRNEHPQIIATILVHLERDQAAEILGYFSERLRADVMLRVA
                     TLDGVQPTALLELNEVLGKVMQGNGNLKKSALGGVRAAAEILNYLGGSLDTAIIESVK
                     EQDADLAQQIVDEMFVFENIIDIDDRGVQLLLREIQSESLVVALKGTSAELREKIFKN
                     MSQRAADMLRDDLEAKGPVRVSEVEAEQKEILKTVRRLVEEGQIALAGKGEESFV"
     gene            13773..14399
                     /locus_tag="VKZ48_03890"
     CDS             13773..14399
                     /locus_tag="VKZ48_03890"
                     /inference="COORDINATES: protein motif:HMM:NF014196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HLU77032.1"
                     /translation="MFIPKEELSAYERWELGSLAEPAAKPKSGATTGAQAPQHAAVPE
                     AELARIRAEAREAGLKEGRAQARQEVERLAKAAAQAEALAGQEAEQLAAEVLDLALEL
                     ARQMVRTELKVRRETLLAVVREALDCLPHATTGAQLQMNPADVDLVRAHAGEELKAGA
                     VRLVEDHRIEPGGCRIVSAACEVDATLATRWKRLVATLGVDHNWLDQS"
     gene            14396..>15243
                     /locus_tag="VKZ48_03895"
     CDS             14396..>15243
                     /locus_tag="VKZ48_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HLU77033.1"
                     /translation="MNDARAPLHRYLQDCKAFAATATPLQTHGRLTRIAGLVMEAVGL
                     KLPLGSTCVVRQDGGYEVEAEVVGFAGDRLFLMPASDPYGLTPGAAVCPLEIKPLPPA
                     YGKPRHPWRRAGDRTKLMPVGPELLGRVVDCAGRPLDNRGPLEASARMPLHSRPANPI
                     DREQITHTLDVGVRAINTLITVGRGQRMGLFAGSGVGKSVLLGMMARYTSADVIVVGL
                     IGERGREVKEFIEEILGAEGLERSVVVAAPADQPPLSRLQGAAYATSVAEYFRDQGQH
                     VLLIMDS"
BASE COUNT         2946 a         4982 c         4846 g         2469 t
ORIGIN      
        1 aagaccgcag acgtaggtat ccgtcagctc ataggtatgc acgacgaact ggccgctgca
       61 gcagctgcag gacgtggtcg tcagcatgcc ggcatcgaag aacttcacca gccgccaggc
      121 gcgcgtgacc gaaagcactt cctccatgcc gtgcgcagcg acgtgttcgc gataaagccg
      181 gtaggccttc accagcacct cgatctgctc cagctccaca gacttgtcga tatagctgta
      241 gatgctcatg aacagcgagg aatggatgtt cggctgccag gtcatgaacc agtcggtcga
      301 aaacggcagc atgcccttgg gcggcgactt gccctgcact tccttgtaca ggcgcagcag
      361 cttctcacgc gacagtctcg tttccgattc gagcacctgc agtcgcgcgc ccagttcgat
      421 cagttcggtc gcgagcttga tgtcgcgggc ttcggcgatg acggacttgg ccatgatctg
      481 ctccggatgc gttgtcgttc ggcttaagcg ggcaccgctt cggcggcggg cgcgttcgcc
      541 atcagcagcg aggcgtgcat ccgggcgatg tcggcgtcct tgctgtggcc ggtcaacagg
      601 ttccacacca tgtcctcgcc gaagcggaag ctgcacatca gcatgttgct ggaggcgatc
      661 ttgatgagtt gcgccatgct caggtcgccg atgatgtccg caacctgctc gttcacgccg
      721 agacggaaca tcgcctgggc gcggtcctcg cggatcatgc gctgcgccag cagcaggtac
      781 gacagattgg cgtcgcggat ttcgtcgagc agttgatctt gtttcatggt gaatcccctt
      841 ttgcgaggga agcggtcacc gcttcctatc cccttaaata cctaccggcc cccgccggcg
      901 tgtgtcgcct cgtcacgaca cgggcccata gtcggccggt aacgaagaaa cttgaatcag
      961 gcttccggcc atttatttcg tgaggcaatg gggacgcggc gatgtaggaa gaaagctgac
     1021 atgtcgacac tgtcagctca ttgacagcga gccgtgcaat cgactcaaac cgctcacgac
     1081 aaagggattt tcgacaaacg ccgaactaca gttgcctgac aagattcgtc gagaccccgt
     1141 caaccggttt gggcgtagtt cacattcgcg cgtgtagggc ctcgcgaacg cgcgcagaca
     1201 aaaaatgtga gcaattcacg aaaacgcaca cgcaacggct aaagaaatcg aatccggcgt
     1261 ttcacgcacc gcattcctaa agttccgccg cggggggacc gataagcagg caacggcggt
     1321 ttgagtcctg acggccaaac caaagtaacc ggcacttggc cggcacatgg gtaaccactc
     1381 attcaggaaa aggaagggac caaagatgcc tcagatcatt aacaccaaca ttgcctcgct
     1441 caacgcgcag cgcaatctga acacttcgca aaccgcgctg gccacctcgc tgcagcgcct
     1501 gtcctccggt ctgcgcatca acagcgccaa ggacgacgcc gcgggcctgg cgatttccga
     1561 gcgcttcacc acgcagatcc gcggtctcac gcaagcggcg cgtaacgcaa gcgacggcat
     1621 ttcgctggcg cagacgggcg aaggcgcgct ggccgaactc ggcgccaacc tgcagcggat
     1681 ccgcgagctg gccgtgcaag ccgccaactc gacgaactcg gcttccgacc gcgccgcgct
     1741 cgacctcgaa gtgcagcagc gcctggaaga gatcaaccgg atcgccaacc agacgtcctt
     1801 caacggccag aagattctgg acggcacctt cggcaccgcc gctttccagg tcggcgccaa
     1861 cgttggcgag accattacca tcaacctgaa caccagcatg cgcggcaccg atatcggcgc
     1921 cgttgcgcaa gccacgtcga gcggcatcgc cgcgctgacg actagcactt ccagcgcatc
     1981 gggcgcgttt accgacgccg tttcgaacgc ggacggccag cggttcacgc tgacagtggg
     2041 caatgtcgtg ctcctcgatg tcgagcagga tggcgctccc gtcacggtca acgacacgat
     2101 gctggatgcc gccttgaccg ccaaggcaga cgaactggcc gaagcaggct tcaccgttac
     2161 cggcaccttc ggcacagaca acgttgtgtt ctcacgcgtg gacggcgccg acttcgatat
     2221 cacggtggac agcaccttca acacaacggg tggctttgcg ggcgccgact ggcccggcac
     2281 cgtctctgca accggtgtgg atttggatgc cggcgatctg accatcgcat tgggcagtgc
     2341 cgaggcggtc gacatcaaag gtgagttctc caccgcgcaa ggattggccg acgcgatcaa
     2401 taccgcggtg gccggcgtca atgcgacggt gaccgaggcg ggtgaaatcc aactgacgtc
     2461 caccggccag aacatcgtca tcggcggcac cgaagcggcc accctgggtc ttacctccgc
     2521 gaccgccagc ggcaacctca gcggggttaa cgtcaaggac gtggatgcag ccaacgacgc
     2581 gatccagcgc atcgactccg cgctgacctc ggtgagcgat ctgcgctcga cgctcggtgc
     2641 gatccagaac cgcttcgagt cgaccatcgc caacctgcag gccacgaccg agaacctgtc
     2701 cgcctcgcgc agccggattc tcgacgcgga cttcgcggcg gagacggccc acctgacgcg
     2761 cggccagatc ctccagcagg ccggcgtggc gatgctggcg caggccaact cgctgcccaa
     2821 caacgtgctg gcgctgttgc gcggttaaca aagcttgaag ctcggatgac gagcaccggg
     2881 tagcaccggc ggcagccaga cggcgggaac ccctatcccc tcccgccgcc cggctgccgc
     2941 acgggctttt gtattactcg aacatcaagg gagtccggtc atgcagatcg acccgctcag
     3001 cattgcgaca accggcatca ccgtgccggc ggccgcgacg cgcccggctc ccgcccccgt
     3061 tgccgccgtc ccgcagtcgc cccccagcga tgcggaactc caggcggcag tggccaagat
     3121 caatcaagta ctgaaggaaa gccgcaacag ccttcagttc gaaatcgacg gcgaaaccgg
     3181 caagacgatc gtgcgcgtga tcgacgagga caccaaggaa gtgctgcgtc agttcccatc
     3241 ggaagaagtg ctggcgattg cccgcgcaat tcccccgatc agcggcgcac tgttgaacga
     3301 tactgcctga gattcagact ccacgcctga ggacccacta tggcgctttc cgcacccggc
     3361 atcggttcca gtcttgacgt caattccatc gttcagcagt tgatggccgt cgagcagcgc
     3421 cccttgcagg cgctcgctca gaaagaggcg agctatcaag ccaagctttc ggcctacggc
     3481 tcgatcaaga gcggcgtatc ggcgttcaag agtgccatgg aagcgctcac ctcgaccgac
     3541 aagttcatca gcagcagggt gacggtcagc aacaaggacg cgctcggcgt gacggcaaac
     3601 gggcaggccg tgcccggccg ttacaacatc gacatccagg cgctcgccca gtcgcagaag
     3661 ctcagctcgg gaaactatgc ggcaacgacg gatacggtcg gtacgggcaa gctgacaatc
     3721 cagttcggca gctacagtga agcggacggc agcttcaccg ccaatgcgga caaagccgcc
     3781 aagaccatcg agatcgaacc gggcaatgcg accctcgccg gtgtgcgcga cgccatcaat
     3841 gcggcggacg ccggcgtgac ggcgacgatc atcaacgacg gcgtcggcta caggttggta
     3901 ctggccgcga aggatacggg gctcgccaac gccatgcgca tcagcgtgag cgacgacggc
     3961 ggctccggcc tggccgcact ggcttacgac gcgagcggcg acggtccgtc gaatctcacg
     4021 gagaccgtac aggcccggaa cgccgaactc accatcgacg gcatcgccat cagcaaggcg
     4081 tccaacacca tttccgacgc catcgacggc gtcacgctca atctgctgga tcaggccgag
     4141 aaaaccatca gcgtgtccat cacccgcgac aacagcggca cgaagacggc ggtagacaac
     4201 ttcgtcaagg cctacaacga tctgtacggt atgttgagca acatgtcggc ctacaacgcg
     4261 gagaccaagc aggcgggcgt gctgcagggc gacagcacca tccgctcgat gcagaccagt
     4321 ctgcgcacca tgatgagcac ggcgctcgac agcgcgggcg gcggcttgag ccgcatgtcc
     4381 gatatcggcg tcaccatcca ggtcgacggc aaactgaagg tcgacagcgc gaagctgcag
     4441 aaagcgatcg acgatccgtc gaaggacatc gccacgctgt tcgcctccat cgccaagccg
     4501 agcgacagtc tggtgtcttt cgtcagcgcc ggcgccgatg ccaaggccgg cacgcatgcg
     4561 ctggagatca cgcagctcgc cacgcaaggc cgcgccgagg gcagccaggc cgccgcgacc
     4621 gccatcacgg ccggcgtgaa cgacaaactc atgctcgaaa tcgacggcgt gaaagcgacc
     4681 attacgctga atgccggcac gtacaccgct acctcgctgg cggcggaact gcaatcgaag
     4741 atcaacggcg cggcggaact gcgcgacaac gacgtcagcg tcgaagtaac cgagtccggc
     4801 ggcgtgctga cgatcacttc cgagcgctac ggctccgcgt ccaaggtgca gatcaccggc
     4861 ggcaatgcaa gaaccggcct gttcggtagc gtgaccagca ccgacggcgt agacgtggca
     4921 ggcaccatgg gcggccaggc ggcgaccggc agcggccgga aactcacggg actgggcatt
     4981 tcgctcgaca tcaccggcgg cgcgctgggt ccgcgcggaa cgatcagctt tgcgcagggt
     5041 ttcgcagagc gtttcgtcaa tctggccgaa cgcttcctcg cggacgacgg cgtgctggat
     5101 gcgcggcagg acgggctcaa ccgctccatc aaggacattg ccgatcgccg tgacgccttc
     5161 aatctgcggc tggaaacgat cgaaaagcgt tatcgcgcgc aattcaccgc actggacgtg
     5221 atgatcgctt cgatgcagca gacctcgagc tatctgcagc agcaactggc cagcctgccg
     5281 aagatcggcg gcgaataaag tgtcgcgaaa gctcaagttc cgcacggatg aggccgatgt
     5341 acaaagtatc tgcaactgat tcggaatgcg tcatgttcgg ttcctttggc accaacgctt
     5401 atgcccaggt cgaggtcgag accggtgtcg cgacggccga tccgcacaag ctggtcggca
     5461 tgctttacga gggcctgctg caatcgatcg cgcggggtcg cgaagcgatg ctggcgaagg
     5521 agatcgcgcg caagggcgag tcgctgtcca aagccgtccg cattctggaa gagggtctgc
     5581 gcggcagcct cgatcgcgaa cgcggcggcg agatcgcaca gcaactggac agcctgtacg
     5641 aatacatgat cacgcggctg ctggcggcga atctgcgcaa cgatctggag gcgctggacg
     5701 aagtgacgcg gctggtcgcc gaattgcgcg atgcctggca ggcgatgccg caggaagcgc
     5761 gcacgtcgat ggccgcggcc tgagacgcat cagaacaaca gagacgaacc acagcggaga
     5821 atgtggatgg gtgtcgaaac gggcgcactg gatttctacg aatcgatcgc cgatgcgagc
     5881 gaacagatgc tgtcggccgc gcggcgggcg gactgggacg cgctcatcga ggcggagaag
     5941 gaatgcgcac gtcgcgtcgc ggcattgcgt gccgccggga tcacgggctc gctcggccgc
     6001 gaagcggacc ggcgccgtca cgacatcctg cgcacggtac tggcgcacga cgccgagatc
     6061 cgtgcactga cccagccctg gatggtcaag ctggaagtgc tgttgaacag cgcggcggcg
     6121 gcacgccgcg tggaacaggc ctaccgctag gcagcgttca accgacgact cgcaccggaa
     6181 ggtgcggcgc aggacgaacg ggagacagat cgaaggaaag ctcatgttcg aactcgaccg
     6241 catccccatc gcaccgacca caccgaccac gccggtgtcg ccgagcacgc ctgcggcgcc
     6301 cgtgcgcccg gtggtgcggc gcgaggaaga cgagcgcgac gaacatgcgc cgcgccgcga
     6361 agaacaccga cgcccgcctc cgccgcccgc gagcccggac ggcctgccgc gcatcgacga
     6421 atacgcccac ggtccttacg gcaacgcctg agtcggcgca tggcgaagct gggtgatgca
     6481 ctgaccgcac tggtgcgcca ggtgcgtttc gacaagctcg gtccgctgct cgatcgcggc
     6541 gcgtctggcg acggcgcaaa gcccgctgcc acagccgcga cgcccgcccc cgtcgatccg
     6601 gtcggcgcca aacaggcgaa tgccggagcc acgtccgacg ccttgcacac gaatgtgcgg
     6661 acgcacgatg tgccgcccgg cccgacatcg ccatcccccg cgccggcacc agcctcggtg
     6721 cagacccgtc tcagcgtgga tgcggcgcgc atcaccgcgc tgctcggcga aacgcccgcc
     6781 gccgcgccca agcagccgct gccggtggcg gcgctcgacg tcgcgacaca atcgcccgag
     6841 caggtcgcgc gagcgctggt gcgctcgctg gccaccagcg gcctgttcta cgaatcgcat
     6901 ttgcaggaat gggtcgaagg gcaacgtccg ctggacgcct tgcgcgccga gccgcagcag
     6961 cgcttcgtgg cgacactgat gcgtgccgcg ggcgcgccgc cggccgggaa cgcggaagcg
     7021 agcgcaccgg catcgggcgg cacgagcgtc cccgcgtcgg cgactgccgc cacgaacgcc
     7081 gcgacagcgc cgctgcccga ggcgctggcc gcgatcgtgc gagagcaact cgacgcactc
     7141 gactttcgcc gcgtccattg gcagggtgag gtgtggccgc aacagccggc ggaactggaa
     7201 atcgccgagg aagacgcacg gcggcgcgac ggcgaggcag cgccctccca acgcggctgg
     7261 gccacgacgc tgcgtctgcg actgccgaag ctgggcgatg tggaagcgcg tatcctgctc
     7321 gacggcgaca gcttcgccgt caacgtgcga gccgacgcga aatcgctgcc ggcactacaa
     7381 tccggccgca ccgctttcgc cgccgccctg aatgaggcgg gcatccgcct gcgcactgtg
     7441 gagttgagcg ggcatgaatg agtacgaacg cgagcaggag cgcccgctcg acgaatcgaa
     7501 cgcgccgctg caaagcgccg tcgccctcgc ctaccgcgaa ggcgatgccg cgccgaaggt
     7561 ggtcgccaag ggacgcggcc tgctcgccga ggaaatcatg cgccgcgcac aggaagcggg
     7621 cgtgtatgtg catcagtcgc gcgaactggt cggcctgctc atgcaggtcg atctggaccg
     7681 ccatattccg ccgcagcttt atcgcgtcat cgcggaattg ctggcctggc tgtaccgtgc
     7741 ggaacaggcg ggcgtgccgc cgtccgccat gccgccgatg ccgggaccgt cacagggagt
     7801 ttgatcgtgg aaggaatgcc gtatcttttt cagcagcgac tcaattccga gctgtacgcg
     7861 cgctatcgca tcgactcgca ggcggaaatc atcgccctgc tgcgttcgct gcaggaagag
     7921 cgcagccagg tggtgctgca cttctccgat cacgcctacg tcgtcagccg cgtgctggaa
     7981 gtctggccgc agcagggacg catcgcactc gacttcggcc ccgacgagac ggtgaacgag
     8041 gcgctgctcg ccgccggcag cgccgtggcc gaaacgaatc tgcaccaggt gtcgatccag
     8101 ttcgagttgg gcgatttccg ccgcgtgatg ctgcacgacg ggccggcgct ggaagcggca
     8161 cttccggcat gcatgctgcg gctgcagcgt cgcgaaacct tccgcgtgcc gacaccggtg
     8221 ctggaaccca ttacgctgtt cgtgcccgag caggaacgct gtccgctgaa tctgcatctg
     8281 cgcgtcgtcg acatcagcgc aggcggcgcg ggcacggtgt gcgacgcgac gcaattccag
     8341 ccggaagccg gcatggtgtt ccgcgactgt cagatgctgc tgccggaagt cggcctgctg
     8401 gtcgccgaca tggaagtacg gcatgtcatg ccgctgcacg aaggcaatac cgatcagttg
     8461 cagtgcggga tgcgtttcct ggcgctgccg ccgccgatgg caagcttcgt gcagcgcttc
     8521 gtgatgcagc tcgaacggga gtggcgcaag ctgcgttgaa agcagtccag agcgaagcgt
     8581 cgagcgtcga gagaaaagcg aacttcttga accacagagg acccacagat cacagagtaa
     8641 aaaccaggat cgatggttat ctctgtgttt cagcttttga ctctcggctt tagcttctca
     8701 gcacgtactt cagaatctcg accacctgat ccgtcgtggt cgcccaggcc agggccgcgg
     8761 cgtccacttc cttcagcgga tggatgatgt cttccgcgtg cagggtgatg tagggcttgc
     8821 ccagcgcggc gcagtagccg gcatcgaaag ccgcgttcca ttgcttgtat ttgtcgccga
     8881 agcgcaccac caccagatcc gccttctcga tcagcgtctt agtacggatg gcgttcacct
     8941 tggacgattt gtggtcgcgc cagaagctgt tcgtttcgac gcccagacag tcgccggccg
     9001 cgtcgctcgc ctcgtgatcg gtcaccggcg cggtaaagac gacgtcgagc cccttcgcgc
     9061 cttccttgat ctgttcgcgc cagtcggtat ggatttcccc ggatagataa acggtaaaag
     9121 acatcaacaa cctccagatt ccgggccgcc gatgtccacg acgacccgtt caagactttc
     9181 gtgccgtcca aggatactca taaatggacg gcaaatcggc caaacccgga ggcggatcgg
     9241 gattcgacgg cgactgaacc ataaaaagcc tggaacacgg agagcacaga acgtaaaacc
     9301 cggctttagc gcctttcggg gaaacgcgcc ggcgttatgc gcacgcaacg aacgacggga
     9361 tttccgcttg cgcgggccgc cccgatttcc gtgtttcagc ttttggggtt tccgcgttcg
     9421 cgggcttcgg gtgttctact caggtatcga acgcgcggct cgcttcggcg cgcagatcgt
     9481 cgaccgcgcc gtcgtcgatg cccagcacgg tgaacacggt ttgcgcagcg agggaccatt
     9541 cgggacagga atggttgcgg ctgtagcggt ggtagatctc ctcggacagc agccccatcg
     9601 ccgcaacacg cacggcgatt tcccgggccc gacccggcca tttatcgtag tcgtggtgca
     9661 gcaggatggc tttccagaca ttgtcgtcca gatgccagct ggtgccgaag tacgcgccga
     9721 cctgcgcatg ggtaaggccg tagcgtgcca gttcgacggc ggtgatcggt gccgtgccgt
     9781 cgaaagcctc gccggtgagg atgtcttcat acgacgcttt cttcttgttc agcatggccg
     9841 gcatgccggc gtcgcggaac agaccgaagg tataggcctg attcgaatcg cacaccttca
     9901 gttcgcgcgc gaccacgtag cagaccgcgg cgacaccggc ggagttcgac cagaagtgct
     9961 ccatggcggc gctttgcgcc acggggaaag cctgccgcag cagcaggccg gtaatgagtt
    10021 gcgcgacgtt ccgcagcccg agcaggatga gcgcctgctg taccgaagtc gccttgttgc
    10081 gcagcccata tagcggcgag ttcaccgttt tgagcatggc cgccgcaagg ccgacgtcgg
    10141 ccgcgaccca ttgcgcgact ttgttcagat ccggatcgtc ggaatgcatt tcccgcacga
    10201 tgcgcgtcag gatcgaagga cagggcggaa tttccagttg tttgaccaga cgatcgactt
    10261 cgccgtcgag gccttcaatc atctgcgcgg tgtcgttcat ggctgcggtt cgcaatgagg
    10321 gggttcaggg gatatcggcc gcctgggcgg aaacttgagg cgcccatcac ccggcgctcg
    10381 gctctccgct ctcgactctc cgctctcggc tctcggctct cggctctcgg ctctccactg
    10441 ccgtcttcac acctgcatgt tcatgatgtc gtgataggcg ctgacgagct tgttgcgcac
    10501 ctgcacgagc gcctggaagc tgatgttggc cttggacgag gcgatcatcg tttcttccaa
    10561 ccccactttc gggtcgttca tcgcataggc gttcgccagt gcatcggcat tctgctgcgt
    10621 ggcgttgatg tcatccagcg ccgccttgaa ggctttgccg aaatccgcct tcgccgcccc
    10681 gcccgcgcct tgcgccggct gcattgcgat cttgttcagg ctcgattcga tgcccgtgat
    10741 tttcatgtcg gccatggccg gactcctttt cgatgccagc gcagactaac agcgctcccg
    10801 ccgtcccagc gcgcggaaat accgtggaaa ggcgcggcta ttcgtcgctt ggcggaccaa
    10861 agtgcctgcc cataatccgc tccattacgg gcaattccgc cccatcttgc tgaaacctat
    10921 ggccgatacc ggaactctcg tcgcgcagca tcagccgccg caattgggcg gcagcttcat
    10981 ctacggcgta cgggccatgc cgccgcagcg cctgttcacc ctgctgaccg gcttcgcgct
    11041 gatcatcgca gtgctggtgg gcatcttcct gtggagccgc acgcccgact atcgcgtgct
    11101 gtacgccaat ctgagcgacc gcgacggcgg cgcggtggtg caggcgctgc agcagatgaa
    11161 ggtgccctac aagttttccg agggcggcgg gatcatctat gtcccttccg acaaggtgca
    11221 cgacgtccga ctgcgcctcg cttctcaagg cctgccgcgc ggcggcgccg tgggtttcga
    11281 attgctggag aaccagaaat tcggcctgac gcaattccag gagcaggtga actaccagcg
    11341 cgcactggaa ggcgaattgt cgcgctcggt gcaatcgctg tccgcggtgc agtccgcgcg
    11401 cgtgcatctg gccattccca aaccgagcgt gttcctgcgc gaacagcaga aacccagcgc
    11461 ctcggtgctg ctcaatctct atgccggtcg cacgctgagc cgcgaacaga ttgccggcat
    11521 tacgcatctg gtgtcctcca gcgtgcccga actgccgacc acctcggtca gcgtcgtgga
    11581 ccagaacggc aatctgctct ccagcgacgg caacccacgt atgcagggtg aactcgacga
    11641 gcagcagctc gcctaccgcc atcaggtcga ggcgagctat atccagcgca tcaccgacat
    11701 cctcgaaccg gtggtcggcc gcgacaacct gcgcgtgcag gtcacggccg atctggattt
    11761 cagccagacc gaagcgacgg cggaaatcta caaacccaat cagaacccgg ccgaagcggc
    11821 catccgcagc cagcagacgc aggagtccgt caacggctcg cagacggccg gcggcgttcc
    11881 gggcgcgctg accaaccagc ccgcgcccgc cgccaccgcc cccattaccg acgcgccgga
    11941 agaaggtgcg tccgccaaca ccacgcaggc gaatctcaac acccatcgcg aatcggtcac
    12001 caactacgag atcgacaaga cggtccggca cacgcgcaca ccgaccggca ccatcaaccg
    12061 cctctcggcc gcggtggtgg tgaactaccg ccgcgcgctg gagccggcgc ccgcgtcagc
    12121 gcccggccag gcggctgcca agcctgtgga aaaactcgaa gcgctgtccg ccgaggaact
    12181 ggcgaagatc gaggcgctgg tcaaggaagc gatgggctac accgccgcgc gcggcgattc
    12241 gctcaatgtg gcgaatgtgc ccttcaccgt accggaggcc gcgcccgcgc ctgtcgagcc
    12301 gccggtctgg aaacagccgc agtacatcga actggcgaag gacgtcggca agaacctgct
    12361 gatcggcgcg ctggtactga tcgtgctgtt cggcttcgtg cggccgctga tccgcacggc
    12421 gcgcaactcc atgaccgtgg cgcccgcgcc ggcgcagttg acggccgcgc cgcaagccgc
    12481 agcggccagt tacgaagaac ggttgcaatc gacacgtgaa ctggcgcgca gcgatccgaa
    12541 gatggtcgcc agcgtggtgc ggaactgggt ggcgcaggat gactgaggac ggcctcaatc
    12601 gcgccgccat tctcctgctt tcgctcggcg agcaggaggc ggcggaagtg ttcaagtttc
    12661 tgggtccgaa ggaggtccag aagctcggcg ccgccatggc caagctgaag aacgtcaagc
    12721 gcgaaaagat cgaggaagtg ctggaggcct tccacgcaga cacgaacgtg cagacttcgc
    12781 tcggcatgga ctcgaacgaa tacatccgtt ccgtgctcac gcgcgcgctg ggcgaggaac
    12841 gcgccacgca tctgatcgac cgcatcctgc agggcggcga caccagcggc atcgagggtc
    12901 tcaagtggat ggaagccggt atggtggccg agctgatccg caacgaacat ccgcagatca
    12961 tcgccaccat cctcgtgcac ctggagcgcg accaggcggc ggagatcctc ggctatttca
    13021 gcgaacgcct gcgcgccgac gtgatgctgc gcgtggccac gctcgacggc gtgcagccga
    13081 cggccctgct ggaactgaac gaagtgctcg gcaaggtgat gcagggcaac ggcaatctga
    13141 agaaaagcgc gctgggcggc gtgcgggcgg cagccgagat cctcaactat ctgggcggct
    13201 cgctcgacac ggcgatcatc gaatcggtca aggagcagga tgccgatctg gcccagcaga
    13261 tcgtggacga gatgttcgtg ttcgagaaca tcatcgacat cgacgatcgc ggcgtgcaac
    13321 tgctgctgcg agaaatccag tccgaatcgc tggtcgtcgc cctcaagggc acctccgccg
    13381 agcttcggga aaaaatcttc aagaacatgt cgcagcgcgc ggccgacatg ctgcgcgacg
    13441 acctcgaagc caaaggcccg gtgcgtgttt cggaagtcga agccgagcag aaggaaatcc
    13501 tcaagaccgt gcgtcgtctc gtggaagaag gccagatcgc gctggcgggc aaaggggagg
    13561 aatcctttgt ttagtcgaga atcgagagtc gagagtggaa agagcaatca cgcctcgcgc
    13621 gtcttgcttg tgatcggccg cactgccgtt cgtcctgagc cggccacgca gtggccgagt
    13681 cgaaggatga acgatcgctc accccgaacg gtgctcccgg ctggattttc gctcgctgct
    13741 ctgccctctc gactctccac tgcttcatga acatgttcat ccccaaagaa gaactgtccg
    13801 cgtacgagcg ctgggaactg ggttcattgg cggaaccggc cgcgaagccc aagagcggcg
    13861 cgacgacggg cgcgcaggcg ccgcagcacg ccgccgtgcc cgaagcggaa ctggcgcgca
    13921 tccgcgccga agcgcgcgaa gcggggctca aggaaggccg cgcgcaggca cgacaggaag
    13981 tcgaacgcct ggcgaaggcc gcagcccagg ccgaagcgct ggccggtcag gaagcggagc
    14041 aactcgccgc cgaagtgctc gatctggcac tggaactggc gcgccagatg gtgcgcaccg
    14101 aactgaaggt gcggcgcgag acgctgctcg ccgtagtgcg cgaagcgctc gactgcctcc
    14161 cgcacgcaac caccggcgcg caattgcaga tgaatcccgc cgacgtggat ctggtgcggg
    14221 cgcatgcggg cgaggaattg aaggccggcg cggtgcgcct ggtcgaggac catcgcatcg
    14281 aacccggcgg ctgccgcatc gtgtcggccg cctgcgaagt ggacgcgaca ctcgccacac
    14341 gctggaagcg gctggtcgcc acgctcggcg tcgatcacaa ctggctggat cagtcatgaa
    14401 cgacgcgcgc gcaccgctgc atcgttatct gcaggactgc aaggccttcg ccgcgacggc
    14461 cacgccgctg cagacgcacg gccggctgac gcgcatcgcc ggcctggtga tggaggccgt
    14521 cggcctcaag ctgccgctgg gcagcacctg cgtcgtccgt caggacggcg gctacgaggt
    14581 ggaagccgaa gtcgtgggtt tcgccggcga ccggctgttt ctcatgccgg ccagcgaccc
    14641 ctacggcctc acgccgggcg cggccgtttg tcccctggaa atcaagccgc tgccgccggc
    14701 ctacggcaag ccgcgtcatc cctggcgccg cgcaggcgat cgcaccaagc tgatgcccgt
    14761 cggacccgaa ttgctcggcc gcgtggtcga ctgcgccggg cggccgctcg acaatcgcgg
    14821 accgctggag gccagcgcgc gcatgccctt gcacagccgc cccgccaacc ccatcgatcg
    14881 cgagcagatc acgcatacgc tcgacgtggg cgtacgcgcc atcaacacgc tcatcaccgt
    14941 gggccgcggc cagcgcatgg gcctgttcgc cggatcgggt gtcggcaaga gcgtgctgct
    15001 gggcatgatg gcgcgctaca ccagcgccga tgtgatcgta gtcggcctga tcggcgaacg
    15061 cggacgcgaa gtgaaggagt tcatcgagga gatcctcggc gccgaaggcc tcgagcgctc
    15121 ggtggtcgtt gccgcgccgg ccgatcagcc gccgctgtcg cgcctgcagg gcgcggccta
    15181 cgcgacctcc gtcgccgaat atttccgcga ccagggtcag cacgtgctgc tgatcatgga
    15241 ttc
//
LOCUS       DATGOU010000069        46701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_6409348, whole genome shotgun sequence.
ACCESSION   DATGOU010000069 DATGOU010000000
VERSION     DATGOU010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 46701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46701
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6409348"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..913)
                     /locus_tag="VKZ48_03900"
                     /pseudo
     CDS             complement(65..913)
                     /locus_tag="VKZ48_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015735.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
     gene            complement(988..1992)
                     /gene="cydB"
                     /locus_tag="VKZ48_03905"
     CDS             complement(988..1992)
                     /gene="cydB"
                     /locus_tag="VKZ48_03905"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HLU77034.1"
                     /translation="MTLDLVPVWTVVLAVAVFMYVLLDGFDLGVGILFPFAPDDESRQ
                     LMMNSVAPIWDGNETWLVLGSMGLFAAFPPAFAIIISALYFPVLLMLLGLLFRGVAFE
                     FRPNARTTRHYWDRAFFLGSLVATFAQGCVLGQFVHGFAVRDGQYIGSTFDWFNPFAL
                     AVGVGLVFGYTLLGATWLVMKTQGELQEWARRKSRIALLGVLAFIALVSLWTPTFEPS
                     IAARWFSWPNVLMLSPVPIVTAAFGWWVWSSLNRGADYQPFFGAMALFAMCYLGLAIS
                     LFPYIVPHAVTLEEAAAAPNTQAFLLLGTLFLIPIILTYTGWSYWVFRGKVRADIGYH
                     "
     gene            complement(1996..3381)
                     /locus_tag="VKZ48_03910"
     CDS             complement(1996..3381)
                     /locus_tag="VKZ48_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013210273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HLU77035.1"
                     /translation="MEADPLFLSRLQFAFLIVFHFLLPAFTIGLASYIAVLEGVHLWT
                     RNPVYLRLSNFWIKIFAVSFGLGVVSGIVMPFQFGTNWSRFSEVTGNIIAPLMGYEGL
                     MAFFLEATFLGVLLFGRSLVPVWAHFGAAVLVAFGTLLSAFWILAANSWMQTPAGFEI
                     VDGVFVATDWMQVIFNPSFPFRFFHTVTAVYLTTAFTVIGVAAYHLLHRQHLQESKIM
                     IAMGFGLASILVPLQLVLGDLHGLNTLEHQPAKLAAMEGIWETGPGQPAILFAIPDEA
                     AERNHAEIGIPNLASLYLTHDWQGEVVGLKDFKREDRPPVAPVFWGFRAMVAMWAIMM
                     ALTVCAWYLALRGQLYERRGFLRACIGGIPSGYIAVTAGWITTEVGRQPWVVHELLRT
                     RDAVTPFLSGGDVIASLTLYVIVYVLIFGAGFYFLVRLARRGPQPLPEDRGPRMGERP
                     ARPLSAATESE"
     gene            complement(3505..4428)
                     /locus_tag="VKZ48_03915"
     CDS             complement(3505..4428)
                     /locus_tag="VKZ48_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HLU77036.1"
                     /translation="MSGNAVQNNGRPPLARYAWLSIGAAIATIALKLLAWWLTDSVGL
                     LSDALESFVNLAAALVTLGMLILAALPPSEQHAYGYSKAEYLASAFEGLLILIAAGAI
                     AVAATMRLLDPRPIDQAVAGIAVSLVASIINFAVARILMQQARAHRSIALEADARHLL
                     TDVWTSAGVVVAVGAVALTGWWRLDPIIALAVAANILWTGATLIYRSVRGLMDEAIPD
                     EQREAVLAVLDGYHDLGVHYHALRTRQAAGRSFISLHVLVPGEWTVKRGHDLAEEIEA
                     GIRAVVPGGCVETHLEPVGDPSSYEDIHLDR"
     gene            complement(4418..4810)
                     /locus_tag="VKZ48_03920"
     CDS             complement(4418..4810)
                     /locus_tag="VKZ48_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018441908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HLU77037.1"
                     /translation="MSGLLLQRLRDQARRLKRETMALYFAARDPATPWYAKAFLMFAV
                     GYALSPIDPIPDFIPVLGLLDELILLPLAFALALKLVPAPVMQAARTRADAQMAGTQP
                     RSYTAAAIIVLLWASVVVLAVWMAHRVW"
     gene            complement(4893..5441)
                     /locus_tag="VKZ48_03925"
     CDS             complement(4893..5441)
                     /locus_tag="VKZ48_03925"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010116987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HLU77038.1"
                     /translation="MGHRLSKITTRTGDAGQTGLGDGSRVAKDDPRITALGDVDELNS
                     ALGLLATEPLPEDVRETLTLVQHQLFDLGGELSIPGYSLVGNAHVATLEERVAHYNAD
                     LPPLKEFILPGGTRAAALAHLARAICRRAERSVVTLADAATPQSRIYLNRLSDLLFIL
                     GRVLNRRAGCGDVFWRKPVSGD"
     gene            5610..6428
                     /locus_tag="VKZ48_03930"
     CDS             5610..6428
                     /locus_tag="VKZ48_03930"
                     /inference="COORDINATES: protein motif:HMM:NF016658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HLU77039.1"
                     /translation="MHRPHAERRGLRHALCALALLCACSAQAAEFDHTHKTWNKLLQE
                     SVVPLNEYKASAVRYNRLGSHGAQYALDWYLGALSAVPTSTYNDWTRAQQLAFLINAY
                     NAYTVKLVLNHYPVNSLKDVVAPGADPWQREFFELLGRQRSLATLERDLIGAFEEPRV
                     HFALSCAAIGCPMLRNEAYVAERLDEQLEDQVRRFMSDATRNRYDAQSNTARVSRIFE
                     WHAKDFARSAGSVQAYIARYAEALTGAPDRGETLAAKPFRIDYLPYDWHLNDAR"
     gene            6818..7777
                     /locus_tag="VKZ48_03935"
     CDS             6818..7777
                     /locus_tag="VKZ48_03935"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLU77040.1"
                     /translation="MDIRSVDLNLLVALDVLLTERNVTRAAARLNLSQSAMSAALARL
                     RTLFNDPLLLRTSGGMLPTTRGEELAAPVKQVLEEIGRLVQPSDGFDPATAKVTFTIA
                     ASDYVQWAILPQLVDFLQRNAPQVGLEVQPSDYAGIGRQLESGDVDLVVINAANAPSA
                     GRSRPLYTERFVVVARRDHPQLKRSMMSVDDFCAVDHVLVARHGHIFAGAAQIDDALA
                     ALGRKRRVRLSVPHFLLVPELVARSDMIAVLPERLARSYGDRLQRFDLPLDVPHLTFA
                     AVWHERTHRNPAQGWLRQALADLMQGTVIESVSAAATPRKRRR"
     gene            7774..8685
                     /locus_tag="VKZ48_03940"
     CDS             7774..8685
                     /locus_tag="VKZ48_03940"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HLU77041.1"
                     /translation="MSLRALLCGGLAALTLASAAAVAQECVPVGVWLRPHDRSVIAEP
                     AVLAVAVRAQAVLLGEQHAHPPQQRWQLQMLRALHARHPQLIVGFEMFPRRVQPALDR
                     WVAGETDVETFIEESDWKKVWRFDFEHYRPLFEYAREHAIPMRALNVERSLVRATGAE
                     GFAAVPAAEREGVSSPADPPRAYVTHLHEIYAEHARSRDAEVDEAAFGRFVEAQLLWD
                     RAMAQGIAEALQQRPDALVVGIMGRGHVQHGHGVPHQLADLGVTRVMSLLPWSADDDC
                     ADLVPGIADAVYGVEAGEREEREAPSN"
     gene            8797..10254
                     /locus_tag="VKZ48_03945"
     CDS             8797..10254
                     /locus_tag="VKZ48_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HLU77042.1"
                     /translation="MDRQIDVVTRLRQGLPADAVLHRIEDTRPYECDGLSAYRELPMV
                     VALPANEEEVAFVLRACHELSVPVIARGAGTGLSGGALPAPDSVLLSLAKMKRILAVD
                     PVARLARVQPGVRNAAISEAAKPYGLYYAPDPSSQIACTIGGNVAENSGGVHCLKYGL
                     TVHNVMRVRGYTMAGEAIEFGSEALDAPGYDLLAAVIGSEGMLAVVTEVTVRLIPLPE
                     AAKVILASFGDVKTAGEAVAAVIAEGIIPAGLEMMDRPATRAVEEFVRAGYDLDAAAI
                     LLCESDGTPEEVEDEIARMEAVLVRNGATNLRVSRDEAERIRFWAGRKAAFPAVGRIS
                     PDYYCMDGTIPRKRLGEVLLFIEEMERKYGLRCPNVFHAGDGNLHPLILFDANDEDQL
                     HRTEAFAAEVLQKCIEVGGTITGEHGVGVEKLNQMCQQFNGDELERFRGVKRAFDPDG
                     LLNPLKNIPTLHRCAEFGRMHVRNGQLPHPELPRF"
     gene            10265..11311
                     /gene="glcE"
                     /locus_tag="VKZ48_03950"
     CDS             10265..11311
                     /gene="glcE"
                     /locus_tag="VKZ48_03950"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017333241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HLU77043.1"
                     /translation="MIAQLADTIRTAAAQQRPLRVHGGNTKRFYGQAVEGEPLDVRAY
                     GGVVDYEPSELVITVRAGTPLAEVERLLAEHHQMLAFEPPHYGAAATIGGAVASGLSG
                     PRRQAVGAVRDFVLGVRMLDGRGEDLSFGGRVMKNVAGYDVSRLMAGALGTLGVLTEI
                     SLKVLPQPQAEATLRFEMPEAVAIQNLNNWAARPLPISASAWQDGELHLRLSGAQVAI
                     DAACARLGGERIDDAGYWQSVRDHTAPFFRADGPLWRVSLPSVARPLELPGAQFIEWG
                     GALRWLVTQADARTVRAAAEARGGHATLFRGGDEETPVFHPLPRAVLDIHRRLKAEFD
                     PHGILNPGRMYRGL"
     gene            11314..12534
                     /gene="glcF"
                     /locus_tag="VKZ48_03955"
     CDS             11314..12534
                     /gene="glcF"
                     /locus_tag="VKZ48_03955"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HLU77044.1"
                     /translation="MQTELADFVRDTEQGRMAEAILRKCVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLMKSMLEGEPVSRSTQLHLDRCLTCRNCETTCPSGVEYGHLVDIGRAIV
                     DAQVERPWHERVLRATIGAVFTRPWLFGPLMHAGMAMRPLLPIRLAQKLPMRIPRAPV
                     RPTGTHARRMVALAGCVQSTLTPDTNAATARVLDRFGITLLEAPGCCGALRFHLDRQD
                     EGLDDMRRMIDRLWPHVEQGVEAFVMTASGCGATVREYGHHLRGDPQYADKAQRISAM
                     TRDLTEVLTDEAARAAPVLREAPLVAFHAPCSLQHWQKLHGVGERLLTALGYRLTPVP
                     DAHLCCGSAGAYSLLQPELSQQLKRNKLAALSSGMPEVILTANVGCQNHLTTEDGIPV
                     KHWIVDLDERLSVR"
     gene            12597..13001
                     /locus_tag="VKZ48_03960"
     CDS             12597..13001
                     /locus_tag="VKZ48_03960"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HLU77045.1"
                     /translation="MSFARTLVLLSATLVSTAALAQQMRPGLWEFSMSGVPMKQTICI
                     TPDMAKDPTSLGKDPQMPDTDCKSSKPTVSGKTTSFTVSCTRPQKMHTKMTLTNNSPD
                     SFTMKQDYDMEMGGQRHRGSTTMNYKRLGDCK"
     gene            13053..14720
                     /locus_tag="VKZ48_03965"
     CDS             13053..14720
                     /locus_tag="VKZ48_03965"
                     /inference="COORDINATES: protein motif:HMM:NF015078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77046.1"
                     /translation="MSATPPSPAQPRVSHHALLWRWFDRNILDLGRQMRWSYLPPLMV
                     YMAAGVSGLTGIVGTFFVKEYLGLSAAFLAALGFWAGLPWALKMPLGHLVDLIWRWKS
                     YLIFLGAGLIAASLLIMIGLISHREAMAAVMKVEAWFVLSVLLAPVGYVVQDVVADAM
                     TVEAVPRVHADGSPVLQDDRKAMHVTMQTLGRVAIIGGTVLVALANVYLFRDAAAMPE
                     AEKVATYARIYTLALIIPVISVGGVVVAQWVRRREVRQLRAHGLSDEEARALLHPHVE
                     RPRVNWWILGGGFGFAAFSLLVGLGDFPAREEVVFLTSMAIIVFMIVKLVRELEPQAR
                     NVLVGTAIVIFVYRAMPATGEGSTWWMIDVLGFDQQFISVLSLIASGLTLLGMFIFRR
                     FMADNSIAYVVGALTIVGTILSLPTLGMYYGVHEWTAAHTNGVVDARFIALVDTALSS
                     PLGQIAMIPMLAWIANSAPENLKATFFAVMASFTNLALSASQLLTKYVNEIWVVQREV
                     KAPGTDEIAVAADYSQLGELFIAVILIGLIVPLATIGIVKLTRFKSA"
     gene            14801..15997
                     /gene="gltS"
                     /locus_tag="VKZ48_03970"
     CDS             14801..15997
                     /gene="gltS"
                     /locus_tag="VKZ48_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HLU77047.1"
                     /translation="MSLTFDPLESLLAAIVCLLLGAAVNRRVGFLSKYNIPDPVTGGL
                     LFAVFVSIAAEWGEAQISLNATLKPVLLLMFFAGVGLGADLRLLKVGGKALALFMVVL
                     FPFLILQNVVGVAAAQALDLHPIFGLIAGSITLVGGHGTGAAYAERFAEVNNLQSVME
                     LSMTSATIGLVLGGIVAGPVAQYLINRHRLRSRSTEATTEFTEPELGPPITALGVMGA
                     LAGILAAVLVGQWLAERFHDLPVTIPGFVWCLIVGVAIRNVAPLVGLHFDDRATELIA
                     GVSLALFLVMTMMALNLVEVVLTAGPLVLIVLLQVVAIVAYAIWVCFRFMGRDYEAVT
                     TTAGFIGFNLGSTATAIANMQAITGKYGPAPKSFLIVPLAGAFFVDLLNAAVLTVFLS
                     LGFIGG"
     gene            16000..17487
                     /locus_tag="VKZ48_03975"
     CDS             16000..17487
                     /locus_tag="VKZ48_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006578872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HLU77048.1"
                     /translation="MRRLLLLLLLGVLTACSRGPDVDMLQRDVEARLAEALPADTLTL
                     EALYRRGSQKDVKSPDGETRRIVYFDVDLKLTKNYDFGAWDSPGVAGVISALGTGPRG
                     LSGIVSGGNRAGDIIRAHGTAIYKQADDGKWSAVAPVGFRNVVAPDVASGAPRPAPEL
                     LLEAMRNVIATAPPETTAATRDVIVQELQVAHAAIQSRLARVQHGYAIAAGANGGQYL
                     RFAQALGDGKTRVVPLITAGGEDNLRMVRALQAPLGMAQGDSALMAYEGKGPFQSEGR
                     YTTLRAIGSLYPEPIHVVVRADSTLRRMSDLVGKRVSIGVPGAASRTTAVAVLEAHGI
                     PLAKLGEVVEFPLNDGLVALRQQEIDALIQVIGFPSASIRDATAAVPLRLLPLDEGAI
                     RKLTAQSPAYFPLTLPRGTYPHQGDAVSTIATAAILVTGTEMTDAEIAGITRMVYARG
                     QDLVARGSAQGAQLSAANAHLGLAVPMHTAAEEVIAELRAASQAP"
     gene            complement(17571..18617)
                     /locus_tag="VKZ48_03980"
     CDS             complement(17571..18617)
                     /locus_tag="VKZ48_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HLU77049.1"
                     /translation="MRKLLALSFCITAMVAAPVQAQPSAEELRTIANQLFKPIPSKVT
                     EVRGRKLTEAQIELGRMLYFEPRLSRSHFISCNSCHAIGTGGADNVPTSIGHGWQKGP
                     RNAPTTLNAVFNIAQFWDGRAADLKEQATGPVEASVEMNNTPDMVVLTLQSIPQYVEM
                     FKKAFPDSKEPVSYDNMARAIEAFEATLITPNSRFDQFLMGKNSLNKNELEGLSLFIN
                     KGCAGCHSGVNVGGQTYWPFGVVAKPGAAVLPESDKGRFAVTQTATDEYVFRAGPLRN
                     IELTAPYFHSGQVWDLEEAVQIMGSSQLGTTLNNNEARLITNFLRTLTGEQPKIEYPI
                     LPPSTISTPKPVAH"
     gene            18784..20535
                     /locus_tag="VKZ48_03985"
     CDS             18784..20535
                     /locus_tag="VKZ48_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HLU77050.1"
                     /translation="MTLTRFGPILRFLAPYKARIAVALTALVVAAGAVLVLGQGLRFV
                     IDAGFGDGSQHRLNYALVGMVAIAALMALASYTRFYLMMSTGERVITDIRRAVFGRIL
                     TLSPGFYEGTRTGEIISRLTNDVTLVQTVIGWGFSMFLRNALMMIGAVIMLIVTSPKL
                     ASLIVVGVPLTLAPILVLGKRIRRMSREVQDRIADVSAHIDEAVHEMRTVQAYVHEDV
                     DRLRFGERAEAAYESAVARIRDRGLLFSSVLLIAFCAIGVILWIGGRDVLAGTLTAGE
                     LSAFVFYAVIVASGAGTVSEVWGDLLRAVGATERIVELLATEPDIVAPAQPLALPARV
                     KGEIVFDRVRFCYPARPDTPALDDFSLRVEAGERVALVGASGAGKSTVLALLLRFYDP
                     QGGSIRIDGFDLAQCDPRAVRRQIALVAQEPVIFAASVAENVRYGRPDASMEDVQAAC
                     EAAYAMEFIEALPQGFDTDLGERGVRLSGGQRQRLALARALLADRPILLLDEATSALD
                     AESERYVQLALERLMRDRTTLVVAHRLATVQSADRIVVMDQGRIVDEGTHNELLRAGG
                     LYARSAALQFLDVAPAG"
     gene            20839..21966
                     /locus_tag="VKZ48_03990"
     CDS             20839..21966
                     /locus_tag="VKZ48_03990"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HLU77051.1"
                     /translation="MQIGIPAETRAGETRVAATPETVKKLVAGGHHKVLVQSGAGVAA
                     AVTDQAFAEAGATIVGSAADVFGQSDIILKVRALTPEEIALTKSGAIVIGLLNPYDTA
                     GIEAMAAKGITGFAMEWLPRISRAQAMDVLSSQANIAGYKAVITAADEYGRFMPMLMT
                     AAGTVKAARVLIMGVGVAGLQAIATAKRLGAVIEAFDVRPETKEQAESLGAKFIEVPL
                     SDEEREAQKSAGGYAREMSDDYKRRQGELVAERAKLADIIITTALIPGRPAPVLIPEE
                     VVQTMKPGSVIVDLAVEQGGNCPLSEPDKVVVKHGVRIVGWTNLPAMLPADASALYAR
                     NLLNFINLLVDTKTGELNLDREDEIIAGSLVCIDGAAVKKG"
     gene            21970..22278
                     /locus_tag="VKZ48_03995"
     CDS             21970..22278
                     /locus_tag="VKZ48_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HLU77052.1"
                     /translation="MELDPIIINLTVFVLAVFVGYHVVWNVTPALHTPLMSVTNAVSS
                     VIIVGAILAAGPEKMDFGGILGAIAVALAAVNVFGGFLVTQRMLEMFKKKQPKSDQGA
                     "
     gene            22301..23710
                     /locus_tag="VKZ48_04000"
     CDS             22301..23710
                     /locus_tag="VKZ48_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HLU77053.1"
                     /translation="MLTLSANQVALLYLVASVLFILTLRGLSHPTTSRRGNLFGMIGM
                     AIAVLTTFSLTQSLVAIIIALVIGGTIGAIVAKKVEMTQMPELVAAMHSLVGLAAVFI
                     AISVINNPTAFNLPAVIPAGNKLELFIGTFVGAITFSGSIIAFGKLAGLGKKFRLFSS
                     APIVFPGQHAVNALMAIAMVGFGIAFFLASPENHWTPYIVMTAIAFVLGVLIIIPIGG
                     ADMPVVISMLNSYSGWAAAGIGFSLNNPLLIIAGSLVGSSGAILSYIMCKAMNRSFIS
                     VILGGFGAEGGAAADDGEQKTVRSGSADDAAFLMSNADSVIIVPGYGLAVARAQHAVK
                     EMAEKLIEKGVNVRYAIHPVAGRMPGHMNVLLAESEVPYDQVFEMEDINSDFGDTDVV
                     FVLGANDVVNPLALEKGSPIYGMPILDAHKARTVLVNKRSMAVGYAGLDNPLFYMDKT
                     MMVFGDAKKVVEDIVKALD"
     gene            23931..25259
                     /locus_tag="VKZ48_04005"
     CDS             23931..25259
                     /locus_tag="VKZ48_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HLU77054.1"
                     /translation="MEIFILFLLVLLNGAFAMSEMAVVSSRKVRLQQWAEEGRAGAST
                     ALALANEPSNFLSTIQVGITVIGVTSGAFGEAALSKDLAAWLSQWPALADAAQGLAAA
                     IVVIGITLASLIIGELIPKRLALLNPERIASIVAGPMRTLALVSYPVVRALSWVTEGF
                     LKLLGVRESAEPFVTEDEIEAMMEQGAEAGIFEAHEQALVKRVFRIDEMRVTGVMTPR
                     SDIVYVDLDDDLQSNMQRIARSHHSRLPAVRGGPESVVGILQVKKLIEDAVAGRPIDL
                     VSKLVKPLFVPQTLTVMETVEAFKKHRQTMALVINEYGELQGIVTLNDVMEALVGDVA
                     TLDHDDERDVVQRADGSWLIEGGVTIERFKDVLDIEGELPGEDEDEYHTLGGFTMKQL
                     GRVPLAGDKFRWLDLEFEVVDMDRNRVDKLIVSRLPPEAAEPAAADESDE"
     gene            25379..26017
                     /locus_tag="VKZ48_04010"
     CDS             25379..26017
                     /locus_tag="VKZ48_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HLU77055.1"
                     /translation="MSTSDEARAAILARIREARGARAAASATPPADGARGPLPPLETD
                     LAARFCARAESMQASTDRVADLREVPAAVAAYLREHDLPFEGCVSPEIAALDWNAAGL
                     ALRAGAARPDDPVGVTTVFAAIAETGTLMLLSGPDTPATASLLPETHIAVAPLARIVP
                     YMEDGWDLLRRERGELPRAVNFVSGPSRTADIDQQIVLGAHGPYRVHIILYG"
     gene            26240..27118
                     /locus_tag="VKZ48_04015"
     CDS             26240..27118
                     /locus_tag="VKZ48_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLU77056.1"
                     /translation="MNQTLTAAVLFADVEGSTALYERLGDAAALSRIGPCMARMKAIV
                     VGGQGRVIKSIGDGIMCMFPNTADALRAAVDMQIAQTDREDGLALRIGFHAGEVILRD
                     GDVYGDTVNVAARIEELAKGEQILTTGPTVALLPEYLKHGLRRVGQVDVRGKEQPVDS
                     FEVIWRWSADLTIMENPVSQQPLPSAQLLLRYRGQELELSGVDREFTIGREPGCDLVT
                     PQPRASRLHGRIEYRGGVFVLLDLSANGTFVQFEGDREFVLRRSFVPLRGRGRIGLGL
                     STTGAGADDALEFRCE"
     gene            27353..28570
                     /locus_tag="VKZ48_04020"
     CDS             27353..28570
                     /locus_tag="VKZ48_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HLU77057.1"
                     /translation="MADTFFLPDWPIEPNQMIWVGLLLLCGIVGGEAVKRYARLPRIT
                     GYTLAGLLLGPGAFGLIGTQLLVELRVFVDISIGLILFELGQRLDLRWLRRTPALLLM
                     GVAESVFAALLVFLCLHYYFAYSALSAAVAGAIAASTSPAVLMRVASELRAEGQVTER
                     ALILAAINSVLAFLALTVLVPWVHLEYEGSALTILLHPFYLLGVSLLLAFVAAWITLR
                     IVVFVGKGAERQFVFVIAMVVLTVGAALALKVPVLLALLAFGAMVRNFDREHHFVVVD
                     FGRASHLFYVILFVVTGAMLDIGLLAAVGGAALAFVLARGLGKAVGIFALAPLTGLST
                     RHASLLVVALAPMSGLAVIMVHETSGMYPQLSAGIAPVVLAAVVILGLLGPVATQYAL
                     RRAGEASPETSRN"
     gene            28577..29698
                     /locus_tag="VKZ48_04025"
     CDS             28577..29698
                     /locus_tag="VKZ48_04025"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HLU77058.1"
                     /translation="MSDPLVFHESQPLTLGIELELQLVNTRDYNLTRAASDLLAALVK
                     REHPGEIKPEITESMIEISTDVCETHAQALHELNILRALLADTAERLNIAVAGGGTHP
                     FQKWSEQRIYDTPRFLHLQELYGYLAKQFTVFGQHVHVGVPSGDEAVRWTQLLSRYVP
                     HFIALTASSPYSLGADTQFDSSRLNVINAFPLSGYMPFVEDWTAFNAYYDTMRGYGIV
                     KSMKDFYWDIRPKPEYGTIELRVCDTPLNIQTAADIAAYAQALCAHFHAHPELRQDRG
                     LMLVYSYNKFQACRFGLEGTLVDPTSQQPVPLAQELIDMLTTVMPYAAERGAGDAIKR
                     LIRLTVARANDARKLREVMEKKDSLTDVVREASRLWMQG"
     gene            29875..30351
                     /locus_tag="VKZ48_04030"
     CDS             29875..30351
                     /locus_tag="VKZ48_04030"
                     /inference="COORDINATES: protein motif:HMM:NF014012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HLU77059.1"
                     /translation="MLQVFSLIITLLLFWLLLSGIYTPFLIGAGIASAVAVTIFARRM
                     EIADREGHPIQLGLGALTYWPWLVKEIVVSALQMTRIILDPRLPISPTVVRFKPSQTT
                     APGLATHANSITLTPGTVTLEASHDEFIVHGITRESAEGAVDSEMDRRVHKLESGQ"
     gene            30348..30632
                     /locus_tag="VKZ48_04035"
     CDS             30348..30632
                     /locus_tag="VKZ48_04035"
                     /inference="COORDINATES: protein motif:HMM:NF015993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HLU77060.1"
                     /translation="MSMFAAAVIGILVALGLALVRALRGPTVFDRVLAANTVGTLAVL
                     MLSVIGFLNGRPEFLDLAIVYALLNVIGTIAVLKFFEFGDLGEPGGDPLR"
     gene            30629..30979
                     /gene="mnhG"
                     /locus_tag="VKZ48_04040"
     CDS             30629..30979
                     /gene="mnhG"
                     /locus_tag="VKZ48_04040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HLU77061.1"
                     /translation="MSTVLDVLSWFCLAAGGLFCIIGAIGLLRMPDLFTRMHATSVIE
                     TLGTGLILLGLALQAGFTLVLAKLVIIALLIFFASPTSSHALAKAALARKLEPQGLDE
                     AGVAALEGAPSSKP"
     gene            30979..31512
                     /locus_tag="VKZ48_04045"
     CDS             30979..31512
                     /locus_tag="VKZ48_04045"
                     /inference="COORDINATES: protein motif:HMM:NF009159.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HLU77062.1"
                     /translation="MINTVLTFMVVLVIGLVMQRNLFAVVVLSGIYSFLMAMVLVGLD
                     AVDVAMTEAAVGAGISTVLLLGALYATGADEAKPMRRPLGPLLLAIVIVGALVYGTLD
                     LPAFSDPEAPIHTHVAPRYLSDTVTETGVPNIVTAVLASWRGFDTLGEVTVVFTAGIG
                     VIALLRRLRRSDKSREG"
     gene            31513..31944
                     /locus_tag="VKZ48_04050"
     CDS             31513..31944
                     /locus_tag="VKZ48_04050"
                     /inference="COORDINATES: protein motif:HMM:NF009162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit B"
                     /protein_id="HLU77063.1"
                     /translation="MERYLILRIVGKLIIPFMLLFGLYVQFHGDFGPGGGFQAGVIAA
                     AGVIFYALIFGLPAARRLVPDRLVETMIALGVLLYAGVGVASMLLGGNYLDYFVLDRD
                     TVHGQHRGIFWVELGVLITVAGVMLKIFYLFASRGRDAADS"
     gene            31947..32315
                     /locus_tag="VKZ48_04055"
     CDS             31947..32315
                     /locus_tag="VKZ48_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HLU77064.1"
                     /translation="MEELASHYSYFVTIVLMVCGLYIVISRGNLIKKLVGLAIFQTSV
                     YLLYIAPGKLIGGTAPIYAEGYGVYSNPLPHVLILTAIVVGVATLALGLALAVRVREA
                     YGSIEEDEILEADAREPERV"
     gene            32315..33787
                     /locus_tag="VKZ48_04060"
     CDS             32315..33787
                     /locus_tag="VKZ48_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D family
                     protein"
                     /protein_id="HLU77065.1"
                     /translation="MMEQLPALQVVLPLIAAPLVVLLRRPNIAWFVAVAASWASLAIA
                     LLLFDHVGQHGVISYAMGSWPAPWGIEYRVDRLNAFVLVLVAGLAAIVAPYSLRSSAA
                     EVPAEQLYLYYALYLLCLAGLLGIVITGDAFNLFVFMEISSLSMYVLIALGRSRRAQL
                     AAYQYLIMGTLGATFYVIGVGMLYLMTGTLNIADLAQRVPEIAGTRAVYAALAFVTAG
                     ISLKLALFPLHFWLPNAYAYAPSAMAAFIAATATKVSVYVLLRYFFFVFGDAVRAGGP
                     ELSGILMALAAVGLVAASLVACYQGDLKRMFAFSSVAQIGYIILGISFANREGLTAAV
                     AHLFNHGITKGALFLLLGMVALRAGGTDFRAVNGLARRMPLTSMGIVLAGFSLIGVPG
                     TAGFITKWYLVLGALALESMALVALIVGSSLIAVVYVWRFVEAAYLREPAADAPGSEA
                     PLSMLIPAWLLVAACIWFGFDTSFNVGAADVAARLMLRQP"
     gene            33784..35232
                     /locus_tag="VKZ48_04065"
     CDS             33784..35232
                     /locus_tag="VKZ48_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D family
                     protein"
                     /protein_id="HLU77066.1"
                     /translation="MTSPLLIALLIPLLGAVGIAAAHRAPNLRETVTLVTAGGLFVAV
                     ASQLPAVLDGARPAVSLFEVFPGVGLAFELEPLGMLFALIASSLWIVNSIYSIGYMRG
                     NDEGHQTRFYVCFALALFGAVGIAFAGNLLTLFLFYEILTLVTYPLVTHHGDAEARQG
                     GRVYLGVLISTSIALMLVGILVTYFSVGTLDFEPGGIIGDRLSDGARGLLLALFMFGI
                     GKAALMPVHRWLPSAMVAPTPVSALLHAVAVVKAGVFCVVKVIVYVFGIDLLAMTANA
                     AWLPYVAGFTIIAASIVALRADNLKRRLAYSTVSQLSYVVLAAALLAPISVIGATLHI
                     AAHAFGKITLFFAAGAIYTASHKTEVSQLAGIGRRMPWTMVAFAIGVLSMIGLPPTGG
                     FISKWFMLAGAMQVEHWIAVGVIAASTLLNAAYFVPIVYTAFMHAPTDTAAHGEAPWP
                     MVLAMVVTASLTVALFFYADVPLALARQLVGV"
     gene            35235..35510
                     /locus_tag="VKZ48_04070"
     CDS             35235..35510
                     /locus_tag="VKZ48_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77067.1"
                     /translation="MARPDPREPHWLTRPDTIRKLWWIFGVVLALTVVAQFVVPLEGH
                     FGADGLFAFNAWFGFLACAALILIAKVLGVLIKRPEDYYQVREEGES"
     gene            35507..37186
                     /locus_tag="VKZ48_04075"
     CDS             35507..37186
                     /locus_tag="VKZ48_04075"
                     /inference="COORDINATES: protein motif:HMM:NF009310.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit D"
                     /protein_id="HLU77068.1"
                     /translation="MNLFHPGLILALGGLLLGALPARARPAATLVLPLLALWALWALP
                     EGILWKGEFLGVPVAPLAVDPLSRLFATIFALMAFAGGLFALRQQSRLEVPAAYIYAG
                     SAIGVTLAGDLATVFVFWELMAVGSTLVIWSGGSAAAWHASKRYFAIHLLGGMLLFAG
                     IMGHVVQTGELYFTRIALDSPAQWLILAGFLINAGAPPLSAWLPDAYPEASWSGMVFL
                     SAFTTKTAVYVLLRGFPGTELLIWVGLYMVMYGIIYALLENDMRRILAYSIVNQVGFM
                     VVGIGIGTEMALNGAAAHAFTHILYKALLLMSAGAVLYQTGKRKCTDLGGLFRTMPLT
                     CICGIVGALAISSFPLTSGFISKSMISEAAVHEHLAFVWYMLAAASAGVFLHAGIKFP
                     WFVFFQRDSGLRPADPPWSMRLAMILLAAACIGLGVFYQPLYALLPYPVDYEPYTAAH
                     VLVYLQLLLFSGLAFFVMLNFLRRTLTITLDFDWFYRVPAMAAVRMVERLFDGVRRGV
                     NATASATSSRLNRLLDRWIGSEGVLARTISTGGMAFWLAAILAGYLVTAYW"
     gene            complement(37209..37988)
                     /locus_tag="VKZ48_04080"
     CDS             complement(37209..37988)
                     /locus_tag="VKZ48_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HLU77069.1"
                     /translation="MSGTEKESKNSIQVIKRMMKLLDVLAQSPNAVSLKVLAASAELH
                     PSTAHRILTAMVNDHMVERVDQGSYRLGMRLLELGNLVKSRISVREQALPLMRELHAQ
                     TNEAVNLSVRRDDEIVYIERSSSGRQLMRVVNIIGEHAPLHITAVGKLFLLEDGPEGI
                     RGYAERTGLPTFTRNSIGTLEALEKELDKVRKTHIAYDNEEAELGVRCIGAGIRDDTG
                     ALVAGLSISAPAERLKPEWAELVKDTADRISRAIGYRGLTL"
     gene            38329..39072
                     /locus_tag="VKZ48_04085"
     CDS             38329..39072
                     /locus_tag="VKZ48_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HLU77070.1"
                     /translation="MKVGLFVTCLVDLMRPSIGFAALTLLEAAGCEVVVPMTQSCCGQ
                     PNYNAGDRKSARRMAAKVLDEFEGVDYIVAPSGSCAGMIRTHYPDLFRDDDPALRARV
                     DALCARTYELTDFLVNVAKLTKVPGRYEGTVTYHDSCSGLRELGVKQQPRALLARVPG
                     LRLTEMDECEQCCGFGGAFAVKFGEISTEIVERKCKAIDASGADAVVLGDLGCMLNIE
                     GRLRRRGDERTQVFHVAEVLANDRKPELE"
     gene            39133..39531
                     /locus_tag="VKZ48_04090"
     CDS             39133..39531
                     /locus_tag="VKZ48_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HLU77071.1"
                     /translation="MAEDAGKLVLRVTLGVLVLLHGIAKVVNGPSHILGMMDQIGMPE
                     VFGYAVYIGEVLAPILVIIGLFTIPAALIIMVNMIFAIVLAHSHQLFSLTKSGGWALE
                     LQGMFLFGALAVALLGAGRYSAGGRYGKWN"
     gene            39522..39998
                     /locus_tag="VKZ48_04095"
     CDS             39522..39998
                     /locus_tag="VKZ48_04095"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLU77072.1"
                     /translation="MELTQAADQAATVTLREVVIANFNEVIRLQPAPGQENFIAPNVL
                     SIAQSKVAPNFAPVAIHAGDDVVGFLMFGQDIETLKWHIVRLMIGAQYQRRGYARTAL
                     RQLIDQLAQRPDCEEIIISFMPGNVAAEKLYESLGFEKTGEFDDEGEVLMRLELKD"
     gene            40124..41551
                     /locus_tag="VKZ48_04100"
     CDS             40124..41551
                     /locus_tag="VKZ48_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HLU77073.1"
                     /translation="MQIRSIHFKAQASRSLANPVLQANLHKFGIGGLALLRARAVEAY
                     GSKAFDTLRDAGEAIRNRTLAELDVWIERFEAEATRRGAKVLYARDDKEAQQLVLEIC
                     RRHEVKKAIKSKSMVSEEVGLNEALIANGVTPIETDLGEYIIQLAGEPPSHIIAPAVH
                     KSKEEVSELFAAKHGTPPKTDNEALTREARAELRQHFLTADLGISGANFLVAETGSGL
                     IVTNEGNGRMVTTLPRVHVSITGIEKVVPTLEDVSTLLRLLPRSATGQPISNYVSFFT
                     GPKGAQDADGPEHMYFILVDNGRTGFLGTDMQEMLRCIRCGACMNHCPVYRTVGGHAY
                     GWVYPGPMGSILTPSYAGMENAPDLPHASTLCNQCGVVCPVRIPLPDLLRKMREKQVQ
                     RRMRPFGEMFALRLWAYAALHPRLYGLAQSFIGGFLRLKAGRGKRINSLPFLGAAWTQ
                     GRDFPRPRNQTFKQKYSRYPGKRTQ"
     gene            complement(41559..43427)
                     /locus_tag="VKZ48_04105"
     CDS             complement(41559..43427)
                     /locus_tag="VKZ48_04105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HLU77074.1"
                     /translation="MGKLYAAVALAALLSAFGPAVAGATLSVSDGQSHYDLTPHVDAL
                     EDPGGTLRVDDVLRLDASFAPAAHDGRDIVRSYSDSAYWFRFTLQSETPRLWLLDLPY
                     SRMDEVQFYAPTGDGGYTLMRAGDSVAPHARPIAHRNLVFPLETSSEPHTFYLRVSTE
                     GTINLHATLRTTENFIAHSQIDTILVGAYFGLLVVMMLYNLLLYLLVRERICLLFALF
                     CLGLSIGQASLHGIGPLLLWPAFPQWSNPAVVYGMSSAGLLSMLFTRELLALNRPVRA
                     FDYVIYGSAIAYGCCALIMAAGFYQPAAIVLSLVGILTPCAAMPVVFYCLRRGYTQDR
                     FAIVAWTVFEIGVATFAARSIGLLPGNPFTDHALEITTALQVFLFALAMAQRMNRMRA
                     ERTRAQSEALEAERALVAALKMSETELEAKVQARTAELAEANRRLRENERHLLHQSQH
                     DALTGLLNRYVLNDRINHALAQRRRDAKALAVLVIDLDGFKPINDRYGHSAGDAALVE
                     TARRLKRCVRDADTVARVGGDEFVVVLESLNDVQEAQSIGDAILQEVARPFSYREHEL
                     SVSASLGIACYPEDGDTAEALIISADAAMYRAKRRGPSLADIAGHQPDAAALGEEA"
     gene            43580..46147
                     /gene="ligD"
                     /locus_tag="VKZ48_04110"
     CDS             43580..46147
                     /gene="ligD"
                     /locus_tag="VKZ48_04110"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HLU77075.1"
                     /translation="MPDHLQRYRQKRDFRITSEPQGGAAAGEGLSFVVQKHAARRLHY
                     DFRLELDGVLKSWAVPKGPSLDPADKRMAVHVEDHPLDYGGFEGVIPPGQYGAGTVIV
                     WDRGIWEPVGDPHEGYRQGKLKFDLYGEKLQGRWTLVRMHGRAGEKQEPWLLIKERDE
                     AARPASEYNIVEELPDSVLGAGSKAKKPARKKTAKRARSKLALPAQARKAELPLTLSP
                     MLATLVDEPPKRGEWVYEVKFDGYRLLARVEGGDIALFTRNGHDWTSKLKSLARAIDA
                     LQLPDCWLDGEIVVQNDQGVPDFQALQNAFDTSRTQDILYYVFDLPYCDGHDLRAVPL
                     RERRALLQRLLQAHPQSRVRYSEDFNAPPEELLDAMCRLRMEGLIGKKADSAYTSRRS
                     PAWIKLKCMQRQEFVIGGYTDPQGSRIGFGSLLLGLYDEQGRLHYAGNVGTGFNDKLL
                     QSLSARLEALRTDEMPFADKPTRGAGFGAGGGKPHWVKPKLVAEVSFGEWTRDGRIRH
                     PVFHGLRTDKPAKAITREKPMHLEEAPAKRKAATKTRAAAKLPAGLKVTNPERVIDPS
                     TGITKLELVQYYARVAPLILPHLKGRPVSLVRAPSGIEGQHIFQKHGAPIPGITHLDP
                     KYDRDHDPLMEIASAEALLNAAQQNCVEYHTWNSTTKAIDQPDRMTFDLDPGEGLKWP
                     QMQEGALLVHGFLEELGLRSFLKTSGGKGLHVIVPLKPGPDYETVKEFSKVIVEHLAR
                     HVPQRFVAKSGPKNRVGKIFADYLRNGRGATTVAAWSARARPGMGVSVPVEWDELPQL
                     RSGDHWTIRTIDERLELDAWREYGKAQRQTLTRAAKALDFELPPAKAAPAKKRKRGA"
     gene            46320..46682
                     /locus_tag="VKZ48_04115"
     CDS             46320..46682
                     /locus_tag="VKZ48_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77076.1"
                     /translation="MPINSAPCKTCRNIQGAADRNDAVVPEIRESDTVIRGGARFYVC
                     PDCEKLYAVKESNEGGTVHYSIVTAASVNRRYQWMVDGADGTRERAKGAMTEEEAKRK
                     YSNPEKIIHRPIYQPPNN"
BASE COUNT         7825 a        15046 c        15231 g         8599 t
ORIGIN      
        1 ggccttttgc tctcgactct taactcttaa ctctcgactg cactgatcac cagtccggat
       61 tgaagctctt gatcacgacg ccgccggatt tttcgtcgtg ttccagttcc agcagttttt
      121 gcgcgcgcgc ctcttcgagc agcttggaaa acgagcggaa gccgtagtag gactcgttga
      181 agcccggttt gcggcgcttg agcgcctgct tgaccatcga accccagatc ttctcttccg
      241 cgccgcgctc ggcatgcagc gcttcgatcg tctccatcac cagatcgatc gcttcctgac
      301 gcctgtcgtc ctcatgcttg ccgtccgtct ccgcgtttgc ggcctcgtgc ttgggtgact
      361 cttcagcctt gtcgccgccg ttcttcgcgg tcgtcttctt cgccgtggat ttcttgcggc
      421 tcttgggctt ggccttctcg cgcaccaggt cgtcgtagaa gatgaattcg tcgcaattgg
      481 cgatgagcag atcggaagtc gatttcttca cgcccacacc gatgacgatc ttgttgttct
      541 cgcgcagctt ggagaccagc ggcgaaaaat ccgaatcgcc ggtaatgagc acgaacgtgc
      601 gcacatgcga tttcgtgtag cagaggtcga gcgcgtccac gaccatgcgg atgtccgccg
      661 agttcttgcc cgactggcgc aggtgcggaa tctcgatcag ttcgaaggag gcttcgtgca
      721 tggcggactt gaacgccttg tagcgttccc agtcgcaata ggccttcttg accacgatgc
      781 tgcctttgag cagcaggcgt tcgagcacct tgttgatgtc gaacttttcg tacttcgcgt
      841 cgcgcacgcc gagcgcaacg ttttcgaagt cgcagaacaa ggccatgttc tgcgtggcgg
      901 tcggttcggt catggcagct ccggctatgc gcaagtgcgc ggaagcgcaa tgctaacccg
      961 cccgcgcggc cggagcatcc ctgtgcctca gtgatagccg atatcggcgc gcaccttgcc
     1021 gcgaaagacc cagtaggacc agccggtgta ggtcaggatg atcggaatga ggaacagcgt
     1081 gccgagcagc aggaaggcct gcgtgttcgg cgccgccgcc gcttcttcca gcgtgaccgc
     1141 atgcggcacg atatagggga agagactgat ggcgaggccg aggtagcaca tggcgaacag
     1201 cgccatggcg ccgaagaaag gctggtagtc ggcgccgcga ttgagggaac tccagaccca
     1261 ccagccgaaa gccgccgtaa cgatcggcac gggggagagc atcagcacat tcggccagga
     1321 gaaccagcgc gccgcaatgc tcggctcgaa agtcggcgtc cacaggctga ccagcgcgat
     1381 gaaggccagc acgccgagca gcgcaatccg cgattttctg cgcgcccact cctgcaactc
     1441 gccttgcgtc ttcatcacca gccaggtggc gcccagcagc gtgtagccga acaccagacc
     1501 cacccccacg gcgagtgcga agggattgaa ccagtcgaag gtgctgccga tgtattgccc
     1561 gtcgcgcacc gcgaagccgt gcacgaactg tccgagcacg cagccttgcg cgaaggtggc
     1621 gaccagcgag ccgaggaaaa acgcgcggtc ccaataatgg cgcgtggtgc gcgcgttcgg
     1681 gcggaactcg aacgccacgc cgcgaaacag caggccgagc agcatcagca ggacggggaa
     1741 gtaaagcgcc gagatgatga tggcgaaagc cggcggaaaa gcggcgaaca agcccatgct
     1801 gccgagcacc agccaggttt cgttgccgtc ccagatcggt gcgaccgagt tcatcatcaa
     1861 ctggcgcgat tcgtcgtcgg gtgcgaaggg aaagaggatg cccacgccca gatcgaaacc
     1921 gtcgagcagc acatacatga acaccgcgac ggcgagcacc acggtccaga cgggaacgag
     1981 atcgagcgtc atggcttact ccgactcggt ggcagccgac agcggacgcg ccgggcgctc
     2041 gcccatgcgc ggaccgcgat cctccggcag cggttgcggc ccgcgccggg cgagacgcac
     2101 cagaaaatag aagccggcgc cgaagatcag cacgtacacg atcacgtaga gcgtcagcga
     2161 tgcgatcacg tcgccgcccg acaagaaggg cgtcaccgca tcgcgcgtgc gcaggagttc
     2221 atgcactacc cacggctgac ggcccacttc ggtggtgatc cagccggcgg tgacggcgat
     2281 gtagcccgag ggaatgcccc ctatgcaggc gcgcaggaag ccgcgccgct catagagttg
     2341 accgcgcagc gccagatacc acgcgcagac cgtcagcgcc atcatgatcg cccacatggc
     2401 caccatggcg cggaaacccc agaacaccgg cgcgacgggc gggcgatctt cgcgcttgaa
     2461 gtccttcagg ccgacgactt cgccctgcca gtcgtgggtc agatagaggc tcgcgagatt
     2521 gggaatgccg atttcggcgt gattcctttc cgccgcctcg tccggaatgg cgaacaggat
     2581 cgccggctgg cccgggcctg tttcccagat gccttccatc gcagcgagct tggccggctg
     2641 gtgttcgagc gtgttgagtc cgtgcagatc gcccagcacc agttgcagcg gcacgaggat
     2701 gctcgccagg ccgaacccca tggcgatcat gattttcgat tcctgcagat gctgccggtg
     2761 cagcaggtga taggccgcca cgccgatgac ggtgaacgcg gtcgtgaggt agaccgccgt
     2821 caccgtatgg aagaaccgga acgggaacga gggattgaaa atcacctgca tccagtcggt
     2881 ggcgacgaaa accccgtcga cgatctcgaa accggcgggc gtctgcatcc aactgttcgc
     2941 cgccagaatc cagaatgccg acagcagagt gccgaaggcg acgagcacgg cggcgccgaa
     3001 atgcgcccag acgggtacca ggctgcggcc gaagagcagc acaccgagga aggtcgcttc
     3061 gaggaaaaag gccatcagtc cttcgtagcc catcagcggc gcgatgatgt tgcccgtcac
     3121 ttcggaaaag cggctccagt tggtgccgaa ctgaaagggc atgacgatgc ccgacaccac
     3181 accgagaccg aaggacaccg cgaagatctt gatccagaaa ttggacaggc gcagatagac
     3241 cggattgcgc gtccacaggt gcacgccttc cagcaccgcg atataggacg caagaccgat
     3301 ggtgaaggcc ggcaacagga agtgaaaaac gatcagaaaa gcgaactgca ggcgcgacag
     3361 gaacagcgga tcggcttcca tcggcatctc ccttctcggg ggcgcaaggc cttgtggccg
     3421 gagagcaaga atccggccct gtcgtcgccg ccgggcaacg tgacggcgac gcccgccttg
     3481 cgtgcaagcg gaatgccgcg atggtcagcg atcgagatgg atgtcttcgt aggaactcgg
     3541 gtcgccgacc ggctccagat gcgtttccac gcagccgccc ggcacgacgg cgcggatgcc
     3601 ggcctcgatt tcctcggcca gatcgtggcc gcgtttgacc gtccattcgc cggggacgag
     3661 cacgtgcagg gaaatgaacg aacgccccgc cgcctgccgg gtgcgcaggg cgtggtagtg
     3721 cacgcccagg tcgtgatagc cgtccagcac cgccagcacg gcttcgcgct gctcgtccgg
     3781 aatggcctcg tccatcaggc cgcggacgga gcgataaatc aaagtggcgc cggtccagag
     3841 aatgttggcg gccacggcca gcgcgatgat cggatccagc cgccaccagc ctgtgagcgc
     3901 gacggcgccg acggcgacca ccacgccggc cgaggtccac acgtcggtga gcagatggcg
     3961 cgcgtcggcc tccagcgcga tggagcgatg cgcgcgcgcc tgctgcatga ggatgcgcgc
     4021 gacggcgaaa ttgatgatcg aggcgaccag cgacaccgcg atgccggcga cggcctgatc
     4081 aatggggcgg ggatcgagca gacgcatcgt cgcggccact gcgatggcgc cggcggcgat
     4141 gaggatgagc aagccttcga aggcgctcgc cagatattcc gccttgctgt agccgtaagc
     4201 atgctgttcg ctgggcggca gcgcggcgag gatgagcatg cccagcgtca ccagcgcggc
     4261 ggccagattg acgaaggact ccagcgcatc ggacagcagg ccgaccgagt cggtgagcca
     4321 ccacgccagc agtttgagag cgatggtcgc gatcgccgca ccgatcgaca gccaggcgta
     4381 tcgcgcgagc ggcggccgcc cgttgttctg cacggcatta ccagacacga tgcgccatcc
     4441 agactgcgag cacgacgacg ctcgcccaca gcagcacgat gatcgccgca gccgtataac
     4501 tccgcggctg cgtgcccgcc atctgcgcgt ctgcgcgcgt gcgagcggcc tgcataaccg
     4561 gcgcgggcac gagtttcagc gccagcgcaa acgccagcgg cagcaggatc aattcgtcga
     4621 gtagaccgag cacggggatg aaatcgggaa tcggatcgat gggcgagagg gcgtagccga
     4681 cggcgaacat gagaaaggcc ttggcgtacc acggcgttgc cggatcgcgc gcggcgaaat
     4741 agagcgccat ggtttcgcgt ttcaatcggc gcgcctgatc gcgcaagcgc tgcaacaaga
     4801 ggccgctcac atcgtcttcg tcttacaccg ggaaagcaca attttacgcg gcaaatcggc
     4861 ccgtttcgcc gaagcgggcc gcatgcgcgt gttcagtcgc ccgacacggg tttgcgccag
     4921 aacacgtcgc cacagccggc gcgccgattg agcacccggc cgagaatgaa cagcagatcc
     4981 gataagcggt tgagatagat gcggctctgc ggtgtggctg cgtcggccag cgtcaccacc
     5041 gagcgctcgg cgcggcggca gatcgcgcgt gccagatgcg ccagcgccgc ggcacgcgtc
     5101 ccgccgggca ggatgaattc cttcagcggc ggcagatccg cgttgtagtg cgcgacccgt
     5161 tcctccagcg ttgcgacgtg cgcgttgccg accaggctgt aacccggaat ggacaactcg
     5221 ccgcccagat cgaacagctg gtgttgcacg agcgtaagcg tctcgcgcac gtcctcgggc
     5281 aggggttccg tcgcgagcag gccgagcgcc gaattcagtt catccacgtc gcccagcgcg
     5341 gtgatgcgcg gatcgtcctt ggccacgcgc gagccgtcgc ccaggccggt ctggccggcg
     5401 tcgccggtgc gtgtggtgat cttggataga cggtgtccca tgaaaggccc tgctgaagcg
     5461 cggacgacta tagcacgcgg ccaccggcgc accgggatga tgcggcacgc cgcttgcccc
     5521 gcagagccgc atcccggatg ggactgacgc gtagtcaatc cgcgtctgtc cggggccgca
     5581 cgccttgtct gccgccgaag gaggaatcca tgcatcgccc ccacgccgaa cgccgcgggt
     5641 tgcgccatgc gctgtgcgcg ctcgctttgc tctgcgcatg cagtgcgcag gccgccgagt
     5701 tcgatcacac ccacaagacc tggaacaagc tgctgcagga aagcgtggtg ccgctcaacg
     5761 aatacaaggc gtcggccgtg cgctacaacc gtctgggcag ccatggcgca caatatgcgc
     5821 tcgactggta tctgggcgcc ttgtcggcgg tgccgacctc cacctacaac gactggacgc
     5881 gtgcgcagca gcttgcgttc ctcatcaatg cctacaacgc ctacaccgtt aagctggtgc
     5941 tgaaccacta cccggtgaac tcgctcaagg acgtggtggc gcccggcgcc gacccctggc
     6001 agcgcgaatt cttcgaactg ctcggccggc agcgcagcct tgcgacgctc gagcgcgatc
     6061 tgatcggcgc attcgaagag ccgcgcgtgc acttcgcgct cagttgcgcg gccatcggct
     6121 gtccgatgct gcgcaacgag gcgtacgtgg ccgagcggct ggacgagcaa ctggaggatc
     6181 aggtgcgtcg cttcatgtcg gacgccacgc gcaaccgcta cgacgcacaa tcgaacacgg
     6241 cgcgcgtatc gcgcattttc gagtggcacg cgaaggattt cgcgcgttcg gcaggatcgg
     6301 tgcaggccta tatcgcgcgc tatgcggagg cgttgacggg cgcgccggat cgcggcgaga
     6361 cgctggcggc gaagcccttc aggatcgatt acctgcctta cgactggcat ttgaacgacg
     6421 cacgctgaaa cctgtacggg ccgtcatcag gtgcggtggg tcgtcaggat ggaaaaaaaa
     6481 gaacctcggg gcggcaaaga ggaggagggg ggccaccccg agggtccaca ggagagattt
     6541 cctgtggccg ccaccgtgca gagggaggga acggtggcgg cttgaatgac gtacggctaa
     6601 gaggaggagg gccgcgcgtc atcaattacg gggctgagtt tagtgcccgg ccgtatattt
     6661 gtccaataga ttagttggat tgtgtatatc catttcctcg ataatcacgc tgcgtgtagg
     6721 ggcttatgcg ggccattttt cctataaaac gcatacgccc gttatatgcg gataaaaatt
     6781 cctcatatgg atgttttcga ggtaaggtat cgacctcatg gatattcgct ccgtagacct
     6841 caatctgttg gtcgcgctgg acgtgctcct gactgaacgc aacgtcaccc gtgcggcggc
     6901 ccgactgaat ctgtcgcagt cggccatgag cgcggcgctg gcgcgcctgc gcaccctgtt
     6961 caacgatccg ctgctgctgc gcacctcggg cggcatgctg cccacgaccc gcggcgagga
     7021 attggcggcc ccggtgaagc aggtgctgga agagatcggg cggctggtgc agccttccga
     7081 cggcttcgat ccggccaccg ccaaagtgac tttcactatc gccgcttccg actacgtgca
     7141 gtgggcgatc ctgccgcagc tggtggattt cctgcagcgg aacgcgcccc aggtcggtct
     7201 ggaggtgcag ccctccgact atgcgggcat cggccgccag ttggagagcg gcgacgtcga
     7261 tctggtcgtg atcaacgcgg ccaacgcgcc gagcgccggt cgctcccgtc cgctttacac
     7321 cgaacgcttc gtcgtcgtcg cccggcgcga tcacccgcaa ctgaagcgct ccatgatgag
     7381 cgtggacgac ttctgcgcgg tcgatcatgt gctggtcgcg cgccacggtc acattttcgc
     7441 cggcgccgcg caaatcgacg acgcactcgc cgcgctcggc cgcaaacgtc gcgtgcgcct
     7501 gtcggtcccg cacttcctgc tcgtgccaga actggtggcg cgttcggaca tgatcgccgt
     7561 gctacccgag cgcctggcgc gcagctatgg cgatcgcttg cagcgtttcg acctgccgct
     7621 cgacgtgccg catctgacct tcgccgccgt ctggcatgag cgcacgcatc gcaatccggc
     7681 gcagggctgg ttgcgtcagg ccctggccga tctcatgcag ggcaccgtca tcgaaagcgt
     7741 gtcggccgcc gccacgccgc gcaaacgccg ccgatgagcc tgcgcgcgct gctgtgcggc
     7801 gggcttgcgg ccctgacgct ggcaagcgcc gctgccgtcg cgcaagaatg cgtgcccgtc
     7861 ggcgtctggt tgcggccgca cgatcgcagc gtcatcgccg agccggcggt gctggcggtg
     7921 gccgtccgcg cacaggcggt gctgctcggc gagcagcatg cgcatccgcc gcagcagcgc
     7981 tggcaactgc agatgctgcg cgcgctgcat gcacggcatc cgcaactgat cgtcggtttc
     8041 gagatgtttc cgcgccgcgt gcagccggcg ctcgatcgct gggtggccgg cgagacggac
     8101 gtcgagacct tcatcgagga atcggactgg aagaaggtct ggcgcttcga cttcgagcac
     8161 tatcgtccgc tgttcgaata cgcgcgtgag cacgccattc ccatgcgtgc gttgaacgtc
     8221 gagcgctccc tcgtgcgcgc cacgggcgcc gagggtttcg ccgctgtgcc ggcggccgag
     8281 cgcgaaggcg tgtcctcgcc ggccgatccg ccgcgcgcct atgtgacgca cttgcacgaa
     8341 atctatgccg agcacgcgcg tagtcgcgac gccgaggtgg acgaagcggc tttcggccgc
     8401 ttcgtcgaag cgcagttgct gtgggaccgc gccatggcgc agggcattgc cgaagcactg
     8461 cagcaacgac ccgatgcgct cgtcgtcggc atcatgggtc gcggccatgt ccagcacggt
     8521 catggggtgc cgcatcaact ggccgatctg ggtgtgacgc gggtcatgtc gctgctgccc
     8581 tggagcgcgg acgacgattg cgcggatctc gtgcccggca tcgccgatgc cgtgtacggc
     8641 gtcgaagccg gcgagcgcga ggaacgcgag gcgccctcga actgaggcgt cccttcgttt
     8701 gacggcgatg agacgaaggg ctatatggag cgggtgagtg ccgaacccca aatccgccag
     8761 ccgtcgccgc gggaactgcc ggttcccggc atcgacctgg atcgccagat cgatgtcgtg
     8821 acgcgtctgc gccagggcct gccggccgat gcggtgctgc atcgcatcga ggacacgcgg
     8881 ccttatgaat gcgacgggtt gtcggcgtac cgcgagctgc ccatggtggt cgcactgccg
     8941 gcgaacgaag aggaagtcgc cttcgtcctg cgcgcctgtc acgaactcag cgtgccggtc
     9001 atcgcgcgcg gtgctggaac cggcctgtcg ggcggcgctt tgcccgcgcc ggacagcgtg
     9061 ctgctgtcgc tggccaaaat gaagcgcatt ctcgccgtcg atccggtggc gcgcctcgcg
     9121 cgcgtgcagc cgggcgtgcg caacgccgcc atttcggaag cggccaaacc ctacggtctc
     9181 tattacgcgc ccgacccttc ctcgcagatc gcctgcacca tcggcggcaa tgtcgcggag
     9241 aactccggcg gcgtgcattg cctgaaatac ggccttaccg tgcacaacgt catgcgcgtg
     9301 cgcggctaca ccatggccgg cgaggcgatc gaattcggca gcgaagcgct cgatgcaccg
     9361 ggctacgatc tgctcgccgc agtgatcggc agcgagggca tgctggccgt ggtgacggaa
     9421 gtgacggtcc ggctcattcc gctgcccgaa gccgccaaag tcatcctcgc ttccttcggc
     9481 gacgtgaaga cggccggcga ggccgtcgcc gccgtgatcg ccgaaggcat catcccggcc
     9541 ggcctggaaa tgatggaccg gcccgcgacc cgcgcggtcg aggagttcgt gcgggcgggc
     9601 tacgatctcg atgccgcggc gatcctgttg tgcgagtcgg acggcacgcc ggaggaagtg
     9661 gaggacgaaa tcgcgcgcat ggaagcggtg ctcgtgcgca acggcgcgac caatctgcgc
     9721 gtctcgcgcg acgaggccga acgcatccgc ttctgggccg gacgcaaggc ggcgttcccg
     9781 gcggtggggc gcatctcacc cgattactac tgcatggacg gcaccattcc gcgcaagcga
     9841 ctgggcgaag tgctgctgtt catcgaggag atggagcgca aatacggttt gcgctgtccc
     9901 aacgtcttcc atgcgggcga cggcaatctg catccgctca tcctgttcga cgccaacgac
     9961 gaggaccaac tgcatcgcac cgaggcgttc gccgccgaag tgctgcagaa atgcatcgaa
    10021 gtcggcggca ccatcaccgg cgagcatggt gtgggcgtgg agaaactcaa tcagatgtgc
    10081 cagcagttca acggcgacga gctggaacgc ttccgcggcg tgaagcgcgc cttcgatccg
    10141 gacggcctgc tcaatccgct gaagaacatt cccactttgc atcgctgcgc cgagttcggc
    10201 cgcatgcatg tgcgcaacgg acagttgccg catcccgagt tgccgcggtt ctgagcgagg
    10261 gccgatgatc gcgcaactgg ccgacaccat ccgcactgcc gccgcgcaac agcgcccgct
    10321 ccgtgtccac ggcgggaaca ccaagcgctt ctacggccag gccgtcgagg gcgagccgct
    10381 cgatgtgcgc gcctacggcg gcgtggtcga ttacgaaccg agcgaactgg tgatcaccgt
    10441 acgcgccggc acgccgctgg cggaagtgga acggctgctt gccgaacacc atcagatgct
    10501 ggctttcgag ccgccgcatt acggcgccgc ggcgaccatc ggcggcgcgg tggccagcgg
    10561 cctgtccggc ccgcgtcgcc aggcggtcgg cgcggtgcgc gatttcgtgc tcggcgtgcg
    10621 catgctcgac ggccgcggcg aggatctgtc cttcggcggc cgggtgatga agaacgtcgc
    10681 cggctacgat gtctcacggc tcatggcggg cgcactgggc acgcttggcg tgctcaccga
    10741 aatctccttg aaggtgctgc ctcagccgca ggccgaggca acgttgcgtt tcgagatgcc
    10801 cgaggcggtc gccatccaga atctcaacaa ctgggcggcg cgtccgttgc ccatcagcgc
    10861 gagcgcctgg caggacggcg aactgcattt gcgcctgtcg ggtgcgcaag tggcgatcga
    10921 tgcggcatgc gcgcgcctgg gcggcgaacg catcgacgac gccggctact ggcagagcgt
    10981 gcgcgatcac accgcgccct tcttccgtgc ggacgggccg ttgtggcgcg tctcgctgcc
    11041 ttcggtcgcc aggcctttgg agttgccggg cgcgcaattc atcgaatggg gcggcgcgct
    11101 gcgctggctc gtcacgcagg ccgatgcgcg cacggtgcgc gccgcagccg aagcgcgcgg
    11161 cggtcatgcg acgctgtttc gcggcggcga cgaggagacg ccggtctttc atccgctgcc
    11221 gcgcgcggtg ctggacatcc atcgccgcct caaggccgaa ttcgatccgc acggcattct
    11281 caatcccggc cgtatgtaca ggggtctgta gtcatgcaga cggagctcgc cgatttcgtc
    11341 cgcgataccg agcaggggcg catggccgaa gccatcctgc gcaagtgcgt gcactgcggc
    11401 ttctgcaccg cgacctgtcc gacctatcaa ttgctgggcg acgaactgga cggaccgcgc
    11461 ggccgcatct acctgatgaa gtcgatgctg gaaggcgagc cggtttcgcg cagcacgcaa
    11521 ctgcatctgg atcgctgtct gacctgccgc aattgcgaga ccacctgtcc gtccggggtg
    11581 gaatacggcc atctggtcga catcggccgc gcgatcgtgg atgcgcaggt cgaacggccc
    11641 tggcatgagc gggtgctgcg cgcgaccatc ggcgcggtgt tcacgcgtcc gtggctgttc
    11701 gggccgctga tgcatgcggg catggccatg cggccgctct tgccgatccg cctggcgcaa
    11761 aaactgccga tgcgcattcc gcgcgcgccc gttcggccga ccggaacgca tgcgcgccgg
    11821 atggtggcgc tcgccggttg cgtgcagtcc acgctcacgc ccgacaccaa tgccgcgacg
    11881 gcgcgcgtgc tggatcgctt cggcatcacg ctgctggaag cgcccggctg ttgcggcgcg
    11941 ctgcgtttcc atctcgaccg gcaggacgag ggcctggacg acatgcgccg catgatcgac
    12001 cgcctgtggc cgcatgtgga gcagggcgtc gaagccttcg tcatgaccgc gtccggctgc
    12061 ggcgcgaccg tgcgcgaata cggccaccat ctgcgcggcg atccgcaata cgccgacaag
    12121 gcgcagcgca tcagcgccat gacgcgcgat ctcaccgaag tactgaccga tgaagccgcg
    12181 cgcgccgccc ctgtgctgag ggaagcgccg ctcgtcgcct ttcacgcgcc ctgcagcctg
    12241 cagcactggc agaagctgca tggcgtgggc gagcggctgc tgaccgcgct gggctatcgg
    12301 ctcacgccgg tcccggacgc gcacctgtgc tgcggctcgg cgggcgccta ctcgctgttg
    12361 cagccggagt tgtcgcagca actgaagcgc aacaagctcg ctgcgctgtc ctcgggcatg
    12421 cccgaagtca tcctcaccgc caacgtcggt tgtcagaatc atctgaccac cgaggacggc
    12481 atccccgtca agcactggat cgtcgatctg gacgaacgac tgtcggtgcg ctgaccattg
    12541 cggttgctgc ggttcccgca taggcttgcg ccacctcaac tcaatgcgga gttctcatgt
    12601 cgttcgctcg cactctggtt ctgctgtccg caacgcttgt ttccaccgct gcgctcgccc
    12661 agcaaatgcg tccgggcctg tgggaatttt ccatgtccgg cgttccgatg aagcagacca
    12721 tctgcatcac gcccgacatg gcgaaggatc cgaccagtct gggcaaggat ccgcaaatgc
    12781 ccgataccga ctgcaagagc tcgaaaccca cggtttccgg caagaccacg agcttcacgg
    12841 tgagctgcac ccggccgcag aagatgcaca ccaaaatgac gctgaccaac aacagcccgg
    12901 acagcttcac catgaagcag gactacgaca tggagatggg cggccagcgt caccgcggtt
    12961 cgaccaccat gaactacaag cgcctgggcg actgcaagta aggctttcct gatgcgcggt
    13021 cggcgggcac gccgatcgcg tacgctttgc tcatgagtgc aacgccgcca tcgcccgcac
    13081 aaccgcgcgt ctcccatcac gcgctgctat ggcgctggtt cgatcgcaac atcctcgatc
    13141 tgggccggca gatgcgctgg tcctatctgc cgccgctgat ggtctatatg gcggcgggcg
    13201 tgtcgggtct caccggcatc gtcggcactt ttttcgtcaa ggagtatctc ggcctctcgg
    13261 ccgcctttct tgccgcactg ggtttctggg ccggcttgcc gtgggcgctg aaaatgccgc
    13321 tcgggcatct ggtcgatctc atctggcgct ggaagtccta tctgatcttt ctcggcgccg
    13381 gcctgatcgc cgccagcctg ctcatcatga tcggcctcat cagtcatcgc gaagcgatgg
    13441 ctgctgtcat gaaagtggag gcctggttcg tcctctcggt gctgcttgca cccgtcggtt
    13501 atgtcgtaca ggacgtggtg gccgatgcga tgacggtgga agccgtgccg cgcgttcacg
    13561 cggacggcag tccggtattg caggacgacc gcaaggcaat gcacgtcacc atgcagacgc
    13621 tcggccgcgt ggcgatcatc ggcggtacgg tgctggtcgc gctggcgaac gtctatctct
    13681 ttcgcgatgc ggccgccatg cccgaagcgg agaaggtcgc cacctatgcg cgcatctaca
    13741 ccctggcgct gatcatcccg gttatttcgg tgggcggtgt ggtcgtcgcg cagtgggtgc
    13801 gccgccgcga agtgcggcag ttgcgcgcgc acgggctgtc ggatgaggaa gcgcgcgcgc
    13861 tgctgcatcc gcatgtcgag cgaccgcgcg tcaactggtg gatcctcggc ggcggcttcg
    13921 gcttcgccgc attctcgctg ctggtgggac tgggcgattt cccggcgcgc gaggaggtcg
    13981 ttttcctgac ctccatggcg atcatcgtgt tcatgatcgt caagctggta cgcgaactgg
    14041 agccgcaggc gcgcaacgtg ctggtgggca cggcgatcgt gattttcgtc tatcgcgcca
    14101 tgccggccac gggcgaaggc tcgacctggt ggatgatcga tgtgctcggc ttcgatcagc
    14161 agttcatctc ggtgctgtcg ctcattgcca gcggcctcac gctgttgggg atgttcatct
    14221 tccgccgctt catggccgac aactccatcg cctatgtcgt gggagcgctc accattgtcg
    14281 gcaccatcct gtcgctgccg acgctcggca tgtactacgg cgtgcacgaa tggacggcgg
    14341 cgcataccaa cggcgtggtc gatgcgcgct tcatcgcgct ggtggatacg gccttgtcct
    14401 cgccgctggg acagatcgcc atgattccca tgctcgcctg gatcgccaac tccgcgccgg
    14461 agaatctgaa agccaccttc ttcgccgtca tggcctcgtt caccaatctg gcgttgtccg
    14521 cgtcgcagtt gctcaccaag tacgtgaacg agatctgggt cgtgcagcgt gaggtgaaag
    14581 cgccggggac ggacgagatc gcggtcgcgg ccgattacag tcaactgggc gagttgttca
    14641 tcgcggtgat cctcatcggc ctgatcgtcc ctctggccac catcggcatc gtcaaactca
    14701 cgcgcttcaa gagcgcttga aggacatggc gcgggttgcc ggaactccgg tacattgcgc
    14761 catccgcccg ctgcgcaagc gctaacagga aggagcgtgg atgtcgctga ctttcgatcc
    14821 gctggaatcg ttactggcgg ccattgtctg cctgctgctg ggtgcagcgg tgaaccgcag
    14881 ggtgggcttc ctcagcaagt acaacattcc cgacccggtg accggcggac tgctgttcgc
    14941 cgtgttcgtg tccatcgcgg cggagtgggg cgaggcgcag atttcgctca acgcgaccct
    15001 gaagcccgtg ctgctcctga tgttcttcgc cggcgtcggc ctgggcgccg acctgcggct
    15061 gctcaaggtg ggcggcaaag cgctcgccct gttcatggtc gtgctgttcc ccttcctcat
    15121 cctgcagaat gtggtgggcg tggccgccgc gcaggcgctc gacctgcatc ccatcttcgg
    15181 tctgatcgcc ggctcgatca cgctggtggg cgggcacggt acgggcgccg cctatgccga
    15241 gcgcttcgcc gaagtgaaca acctgcagtc ggtgatggaa ctgtccatga cttcggcgac
    15301 catcggcctg gtgctgggcg gcatcgtcgc cggcccggtg gcgcaatacc tgatcaatcg
    15361 ccacaggctg cgttcgcgtt ccacggaagc aacgacggaa tttacggagc ccgagctcgg
    15421 gccacccatc accgcgctgg gcgtgatggg tgcgctggcg ggcatcctcg ccgcggtgct
    15481 ggtcgggcag tggctggcgg agcgtttcca cgatctccca gtgaccattc ccggctttgt
    15541 atggtgtctg atcgtcggcg tggcgatccg caacgtggcg ccgcttgtgg ggctccactt
    15601 cgacgaccgc gccaccgagc tgattgcggg cgtgagcctg gcgctgtttc tcgtcatgac
    15661 catgatggcg ctcaatctgg tcgaagtcgt gttgaccgcg ggacccctgg tgctgatcgt
    15721 gctgttgcag gtggtggcca tcgtggccta cgcgatctgg gtgtgcttcc gcttcatggg
    15781 ccgcgattac gaagcggtaa ccaccactgc cggtttcatc ggcttcaatc tgggctcgac
    15841 ggccacggcc attgccaaca tgcaggccat tacgggcaag tacggtcccg cacccaagtc
    15901 ctttctgatc gtgccgcttg ccggcgcgtt cttcgtggat ctgctgaacg cggcggtgct
    15961 gacggtgttc ctttcgctcg ggttcatcgg aggctgaaca tgcgcagact cctgctcctt
    16021 ctcctgctcg gtgtgctgac ggcctgcagc cgcggaccgg acgtcgacat gctgcaacgc
    16081 gacgtcgaag cgcgtctggc cgaggcgctg cctgccgaca cgctgacgct cgaagcgctt
    16141 tatcgccgcg gttcccagaa ggacgtcaag tcgccggacg gcgagacccg ccgcatcgtt
    16201 tatttcgacg tcgatctcaa gttgacgaag aactacgact tcggcgcctg ggattcgccc
    16261 ggcgtggcgg gcgtgatcag tgcgctgggc accggcccgc ggggactgtc cggcatcgtg
    16321 agcggcggca accgggcggg cgacatcatc cgcgcgcacg gcacagccat ttacaaacag
    16381 gcggacgacg gcaaatggag cgccgtcgcg ccggtggggt tccgcaatgt ggtggcgccg
    16441 gatgtcgcca gcggtgcgcc gcgtcctgcg cccgaattgc tgctcgaggc gatgcgcaat
    16501 gtcatcgcca cggcgccgcc cgaaaccacc gccgcaacgc gcgacgtcat cgtgcaggaa
    16561 ctgcaggtgg cgcatgccgc catccagtcg cgcctggcac gcgtgcaaca cggttatgcc
    16621 atcgcggcgg gcgccaacgg cggccagtac ctgcgcttcg cccaggcact gggcgacggg
    16681 aagacgcggg tggtgccgct gatcaccgcg ggcggcgaag acaatctgcg catggtgcgc
    16741 gcgctgcagg cgccgctcgg catggcgcag ggcgattcgg cgctgatggc ttacgagggc
    16801 aaggggccgt tccaaagcga aggccgctac acgaccctgc gcgccatcgg cagcctttac
    16861 cccgaaccga ttcatgtcgt ggtgcgcgcc gacagtacgc tccgccgcat gtccgatctg
    16921 gtcggcaagc gggtgtcgat cggtgttccg ggcgccgcct cgcggaccac ggccgtcgcc
    16981 gtactggaag cgcacggcat tccgctcgcg aagctgggcg aggtcgtgga atttcccttg
    17041 aacgatgggc tggtggcatt gcgccagcag gaaatcgacg cgctgatcca ggtgatcggc
    17101 ttcccttcgg ccagcattcg cgacgccacg gcggcggtgc ctctgcggct gttgccgctg
    17161 gacgagggtg ctatccgtaa actcaccgcc caaagcccgg cgtatttccc gctcaccttg
    17221 ccgcgcggca cttatccgca tcagggcgat gccgtgtcca ccatcgccac cgctgccatc
    17281 ctggtgaccg gcacggaaat gaccgatgcc gaaatcgccg gcattacgcg gatggtctat
    17341 gcgcgcgggc aggatctggt cgcgcgcggc agtgcgcagg gcgcgcagct ttcggccgcg
    17401 aatgcgcacc tggggctggc ggtgccgatg cacacggcgg ccgaagaagt cattgccgaa
    17461 ctgcgggcgg catcgcaggc gccatgaaaa acggccaccg cccgtgagga cggcagccgc
    17521 gcgaggaaga cggggtcgcc gcgaagcgcg cggcggcctg ggcgcccggc tcagtgagcc
    17581 acgggcttgg gtgtgctgat ggtgctgggc ggcaggatcg ggtattcgat cttcggttgc
    17641 tcacccgtca gcgtgcgtag gaaattggtg atcaggcgcg cttcgttgtt gttcagcgtc
    17701 gtgcccaatt gcgacgaacc catgatctgc acggcctctt ccagatccca cacctggccg
    17761 ctgtggaagt agggcgcggt cagttcgatg ttgcgcagcg gaccggcacg gaacacgtac
    17821 tcgtcggtcg ccgtctgcgt caccgcgaag cggcccttgt cgctttcggg cagcactgcg
    17881 gcgccgggtt tggcgaccac gccgaacggc cagtaggtct gcccgcccac gttcacgccg
    17941 ctgtggcagc cggcgcagcc cttgttgatg aacagcgaca aaccttccag ttcattcttg
    18001 ttgaggctgt tcttgcccat caggaactgg tcgaaccgcg agttgggcgt gatcagcgtc
    18061 gcttcgaagg cctcgatcgc ccgcgccatg ttgtcgtagc tcaccggctc cttgctgtcg
    18121 gggaaggcct tcttgaacat ttccacgtac tggggaatgc tctgcagcgt gagcacgacc
    18181 atgtccggcg tgttgttcat ttccacgctc gcctcgaccg gcccggtcgc ctgctctttc
    18241 aggtcggcgg cgcggccgtc ccagaactgc gcgatgttga ataccgcgtt gagggtggtc
    18301 ggcgcgttgc gcgggccctt ctgccagccg tggccgatgg aagtcggcac gttgtctgcg
    18361 ccgccggtac cgatggcgtg gcagctattg cagctgatga agtggctgcg cgacaggcgc
    18421 ggctcgaaat agagcatgcg gcccagctcg atttgcgctt cggtcagctt cctgccgcgg
    18481 acttccgtca ctttcgaggg gatcggcttg aacaactggt tggcgatggt tcgcagctcc
    18541 tctgccgagg gttgcgcctg caccggcgcg gcgaccatcg cggtgataca gaacgacaaa
    18601 gcgagcagct tgcgcatgga tattcccttt cagcgaagtg gttattgcta ttgaactaca
    18661 cacccgggaa acaagcttga tcgcgatcaa acaaaaatcg ttccttctcc ggccggtcgg
    18721 cggcgccttt tccgcagccg cgcccgggct ttacggccat tttccgtcga ggcacccgat
    18781 cgcatgacac tgacccgttt cggccccatc ctccgctttc tcgcgccgta caaggcacgc
    18841 atcgccgtcg cactgacggc gctcgtcgtc gccgcgggcg ccgtgctggt gctggggcag
    18901 ggactgcgtt tcgtcatcga tgcgggtttc ggcgacggca gccagcaccg gctcaactac
    18961 gcgctggtcg gcatggtggc catcgccgcc ctcatggcgc tcgccagcta cacccgcttc
    19021 tatctgatga tgagcaccgg cgagcgcgtc atcaccgaca tccgccgtgc ggtgttcggt
    19081 cgcatcctca cgctgtcgcc gggtttttac gaaggcacgc gcaccggcga gatcatttcc
    19141 cgcctgacca acgacgtcac cctggtgcag accgtgatcg gctggggctt ctcgatgttc
    19201 ctgcgcaacg cgctgatgat gatcggcgcg gtgatcatgc tgatcgtcac cagtcccaag
    19261 ctcgcgtcgc tgatcgtggt gggcgtgccg ctcacgctcg cacccatcct cgtgctgggc
    19321 aaacgcatcc ggcgcatgtc gcgcgaagtg caggatcgca tcgccgacgt gtcggcgcac
    19381 atcgacgaag ccgtgcacga aatgcgtacc gtacaggcct atgtgcatga ggacgtcgat
    19441 cgcctgcgct tcggcgaacg cgcggaggca gcatacgaat ccgccgtcgc acgcatccgg
    19501 gatcgcggcc tgctgttctc ctcggtgctc ctgatcgcct tctgcgccat cggcgtcatt
    19561 ctgtggatcg gcggacgcga cgtgctggcc ggcacgctca ccgcgggcga actgtccgcg
    19621 ttcgtgttct atgccgtgat cgtcgccagc ggtgcgggca cggtctccga agtgtggggc
    19681 gacctgctgc gcgcggtcgg cgctacggag cgcatcgtgg aactgctggc caccgagccg
    19741 gacatcgtcg cgcccgcgca accgcttgca ctgcctgcgc gcgtgaaggg cgagatcgtc
    19801 ttcgaccggg tgcgtttctg ctatccggcg cggcccgata cgccggcgct ggacgacttc
    19861 agtctgcgcg tcgaagcggg cgagcgcgtg gcgctggtcg gtgcatcggg cgcgggcaaa
    19921 tcgaccgtgc tcgcattgct gctgcgcttt tacgatccgc agggcggcag catccgcatc
    19981 gacggtttcg atctggcgca atgcgatccg cgcgccgtgc gtcgacagat cgcgctggtg
    20041 gcgcaggagc cggtgatctt tgccgcctcg gtcgcggaaa acgtgcgcta cggtcggccc
    20101 gatgcctcga tggaagacgt acaggctgcc tgcgaagcgg cgtatgccat ggaattcatc
    20161 gaggcgctgc cgcagggctt cgacaccgat ctgggcgaac gcggcgtgcg gctctccggc
    20221 gggcagcgtc agcgtctagc gctcgcccgc gcgttgctgg ccgaccggcc catcctgctg
    20281 ctggacgagg cgaccagcgc cctcgatgcc gaaagcgagc gctatgtgca actcgcgctg
    20341 gaacgcctca tgcgcgaccg caccacgctg gtcgtcgcgc atcggctcgc gaccgtgcag
    20401 agcgccgacc gcatcgtcgt gatggatcag ggccgcatcg tcgacgaggg gacccacaac
    20461 gagttgttgc gtgccggcgg gctttacgca cgttcggcgg ccttgcagtt tctggacgtg
    20521 gcacccgccg gttgaaccgc ttcggggatt tagcatgcat caaaagtgac ttaccccttt
    20581 tggtcccata agtcggtgca cgtagtcaaa acaatattga ctggggtatc atcccgcagt
    20641 tttttggaag gccgtcccag gcgcttcgtc gaactccgtc gcgggggatg cccgttccgc
    20701 agttttcgtt cgagcccgca tccgtcccgc attcctcgcg caggcggttg tttttaaaag
    20761 gcctttctgg ttccgcctcg tcccgcgcag tgaacaacgc ggccgaggtg cgtctatttc
    20821 aaacataggg gagcatccat gcagatcggg atacccgctg aaactcgcgc gggcgaaacg
    20881 cgcgtcgcag cgacccctga gaccgtcaag aagctcgtcg ccggtggtca ccacaaagtg
    20941 ctcgtacagt cgggcgcggg tgtagccgcc gcggttacgg atcaggcgtt tgccgaagcc
    21001 ggcgcgacca ttgtcggctc ggcggccgac gtgttcggcc aatcggacat cattctcaaa
    21061 gtacgggcac tcacgccgga agaaatcgcg ctgaccaaat ccggcgcgat cgtcatcggc
    21121 ctgctgaatc cgtacgacac cgcgggcatc gaagcgatgg ccgccaaagg catcaccggc
    21181 ttcgcgatgg agtggctgcc gcggatttcc cgtgcgcagg cgatggacgt cctgtcctcg
    21241 caagccaaca tcgccggcta caaggcggtg atcaccgccg cggacgagta cggccgcttc
    21301 atgcccatgc tgatgaccgc cgccggtacg gtgaaagccg cgcgcgtgct catcatgggt
    21361 gtcggcgttg ccggtttgca ggcgatcgcg acggccaagc gcctgggcgc ggtcatcgaa
    21421 gccttcgacg tgcgtcccga aaccaaggaa caggccgaat cgctgggcgc caagttcatc
    21481 gaagtgccgc tgtccgacga agagcgtgaa gcgcagaaga gcgccggcgg ctacgcccgc
    21541 gagatgagcg acgactacaa gcgtcgccag ggcgaactcg tcgccgagcg cgcgaagctg
    21601 gccgacatca tcattaccac cgcgctcatc ccgggccgtc cggcgccggt gctgatcccg
    21661 gaagaagtgg ttcagaccat gaagcccggt tcggtcatcg tcgacctcgc ggtggagcag
    21721 ggcggcaact gcccgctgtc cgagccggac aaggtcgtcg tcaagcacgg cgtgcgcatc
    21781 gtcggctgga ccaacctgcc cgcgatgctg ccggcagacg ccagcgcgct gtacgcccgc
    21841 aacctgctca acttcatcaa cctgctcgtc gatacgaaga ccggtgaact caacctggat
    21901 cgggaagacg agatcatcgc cggttcgctc gtctgcatcg acggcgcggc agtgaagaaa
    21961 ggttaagtta tggaactcga tcccatcatc atcaacctga ccgtgttcgt gctggccgtg
    22021 ttcgtcggct atcacgtggt ctggaacgta acgcccgcgc tgcacacgcc gctcatgagc
    22081 gtgaccaacg cggtgtcttc cgtgatcatc gtcggcgcga ttctcgcggc cggccccgaa
    22141 aaaatggatt tcggcggcat cctcggtgcg atcgcggtgg cgctggctgc cgtgaacgtc
    22201 ttcggcggct tcctcgtcac ccagcgcatg ctggagatgt tcaagaagaa gcagcccaag
    22261 tcggatcagg gcgcctaagg cgacaaggag ctgaagaaaa atgttgaccc tgtctgccaa
    22321 ccaagtcgcc ctgctgtacc tcgtcgcgtc ggtgctgttc atcctgacgc tcaggggcct
    22381 gtcccacccg accacctcgc gtcgcggtaa cctgttcggc atgatcggca tggccatcgc
    22441 cgtgctgacc accttctcgc tcacgcagag cctggtcgcg atcatcatcg cgctcgtcat
    22501 cggcggcacc atcggtgcga tcgtggcgaa gaaggtcgaa atgacccaga tgccggaact
    22561 ggtcgcggcc atgcactcgc tggtcggtct ggccgcggtg ttcatcgcga tttccgtgat
    22621 caacaacccg accgcgttca acctgccggc cgtcattccc gcgggcaaca agctggagtt
    22681 gttcatcggt accttcgtcg gtgcgatcac cttctccggc tccatcatcg ccttcggcaa
    22741 gctcgccggc ctgggcaaga agttccgcct gttctccagc gcgccgatcg tgttccccgg
    22801 ccagcatgcg gtgaacgcgc tcatggccat cgccatggtc ggtttcggta tcgccttctt
    22861 cctggcctcg ccggagaacc actggacgcc gtacatcgtc atgaccgcca tcgccttcgt
    22921 tctcggcgtg ctgatcatca tcccgatcgg cggtgcggac atgcccgtgg tgatttcgat
    22981 gctgaacagc tactcgggct gggcggcggc cggtatcggc ttctcgctca acaacccgct
    23041 gctgatcatc gccggttcgc tggtcggctc ctcgggtgcg atcctgtcct acatcatgtg
    23101 taaggccatg aaccgctcgt tcatcagcgt cattctcggc ggcttcggcg ccgagggcgg
    23161 cgctgcggcc gacgacggcg agcagaagac cgtgcgttcc ggctcggccg acgacgcggc
    23221 cttcctcatg tccaacgccg acagcgtgat catcgttccg ggttacggcc ttgccgttgc
    23281 ccgcgcgcag cacgccgtga aggaaatggc cgagaaactg atcgagaagg gcgtgaacgt
    23341 gcgctacgcc atccaccccg ttgcgggccg gatgccgggc cacatgaacg tgctcctggc
    23401 cgaatcggaa gtgccgtacg accaggtctt cgaaatggaa gacatcaaca gcgacttcgg
    23461 cgacaccgac gtggtcttcg tgctcggcgc gaacgacgtg gtgaacccgc tggcgttgga
    23521 gaagggcagc ccgatctacg gcatgcccat cctcgatgcg cataaggcgc gcacggtgct
    23581 ggtcaacaag cgttccatgg ccgtgggtta cgccggtctg gacaacccgc ttttctacat
    23641 ggataaaacc atgatggtgt tcggcgacgc gaagaaagtc gtggaagaca tcgtcaaagc
    23701 gctcgactaa caacaacaag cgccttggag ggagcctgcg ggctcccttt tttatttgtg
    23761 tgggcccgga gccggaaagc cgggtgatcg acggccgcgg attggcgcgg ctgatattgc
    23821 cgccgcgttg cgagcggcgg gctttttgac tgggtcagat cggccttgtg ccaaactggg
    23881 gccttccggg ctcgcctcga gccgatcttt gcccgcttcc gctacctgcc atggaaatct
    23941 tcattctgtt tttgctggtc ctgctcaacg gcgctttcgc gatgtcggaa atggcggtgg
    24001 tttcgtcgcg caaggtgcgc ctgcagcaat gggcggaaga agggcgcgcg ggcgccagca
    24061 ccgcgttggc gctggccaat gagccctcca attttctttc gaccatccag gtcggtatta
    24121 ccgtgatcgg cgtcaccagc ggcgctttcg gtgaggcggc gctgtcgaag gatctggcgg
    24181 catggctgtc gcaatggcct gctctggcgg atgctgcgca gggcctggcc gccgctatcg
    24241 tggtcatcgg catcaccctg gcctcgctca tcatcggcga gctcattccc aagcggctgg
    24301 cgctgctcaa tcccgagcgc attgccagca tcgtcgccgg gccgatgcgc acactcgccc
    24361 tggtgagcta tcccgtcgtg cgggccttga gctgggtgac cgagggtttt ctcaagctgc
    24421 tgggcgtgcg cgaaagcgcg gagcccttcg tgacggagga tgagatcgaa gccatgatgg
    24481 agcagggcgc cgaggcggga atcttcgagg cgcacgagca ggcgctggtg aagcgcgtgt
    24541 tccgtatcga cgaaatgcgc gtcactggcg tcatgacccc gcgcagcgat atcgtctacg
    24601 tggatctgga cgacgatctg caaagcaata tgcaacgcat tgcccgaagt caccactccc
    24661 gcctgccggc cgtgcggggc gggccggaga gcgtggtggg cattctgcag gtgaagaaac
    24721 tcatcgagga tgccgtggcg gggcgtccca tcgatctggt ctccaagctc gtcaagccgc
    24781 tgttcgtgcc gcagacgctc accgtcatgg aaacggtcga agccttcaag aagcaccggc
    24841 agaccatggc gctggtgatc aacgagtacg gcgagttgca gggcatcgtg acgctcaacg
    24901 acgtgatgga ggccctggtc ggcgatgtcg ccacgctgga tcacgatgac gagcgcgatg
    24961 tggtgcaacg cgccgacggc tcctggctga tcgaaggcgg tgtgacgatc gagcgcttca
    25021 aggacgtgct cgatatagaa ggggaactgc cgggggaaga cgaagacgag tatcacaccc
    25081 tcggcggctt tacgatgaag caactgggcc gcgtgccgct cgccggcgac aaattccgct
    25141 ggctcgatct ggaattcgaa gtcgtggata tggatcgcaa ccgggtggac aagctcattg
    25201 tcagccggct gccgccggag gcggccgaac cggcggcagc ggatgaatcg gacgagtaat
    25261 gcgacttggc gccttggttc ggcagcgtcc tgccgccgtc cccgcgcgcg ccgctttcac
    25321 tgcttgtagc gcatcgcata cactcgaccg atcccacgtc ataccgagga gcggagtcat
    25381 ttcaaccagc gacgaagccc gtgcggcgat tctggcgcgc atccgcgagg cgcgaggcgc
    25441 ccgtgcagcc gcttccgcga ccccgcctgc cgacggcgcg cgtggcccgc tgccgccact
    25501 cgaaaccgat ctcgccgccc gcttctgtgc ccgcgcggag tcgatgcagg ccagcaccga
    25561 ccgcgttgcg gacttgcgtg aggtgccggc tgccgtcgcc gcctatctgc gtgagcacga
    25621 cttgccgttc gagggctgcg tgtcgccgga aatcgccgca ctggattgga atgccgccgg
    25681 actcgcgctg cgcgcgggtg cggccagacc ggacgatccg gtcggcgtga cgacggtttt
    25741 tgccgccatc gccgagaccg gcacgctgat gctgctgtcc ggccccgata cgccggcaac
    25801 cgcgagcctc ctgcccgaaa cgcatatcgc cgtggcgccg ctcgcgcgca tcgttccgta
    25861 tatggaggac ggctgggatc ttttgcgccg cgaacgcggc gaattgccgc gtgcggtcaa
    25921 cttcgtttcc ggtccctcgc gcaccgcgga catcgatcag cagatcgtcc tcggcgcgca
    25981 cgggccgtat cgcgtacata tcattctgta cggctagcgg gaccgaccat gtttcatgac
    26041 attgtcacgc gttctggtca accgcagccc agtcgcgcta atctatgcgg cgtcgtcggt
    26101 ccggaagcgc agccgtcgca ggcttgcgcc acggactgtt tgccgctcgt acatcgagta
    26161 acaagccgag ccgtgcaggg cggcgtcccg gccgcatgaa gccgggcgaa tgggtcgcac
    26221 ggccgcaaaa gaacgttcca tgaatcaaac gctgactgcc gcggtgctgt ttgcagacgt
    26281 cgaaggaagc accgcgctgt acgagcgatt gggcgacgcc gcggcgttgt cgcgcatcgg
    26341 tccgtgcatg gcgcgcatga aggcgatcgt cgtgggcggc cagggccgcg tcatcaaaag
    26401 catcggcgac ggcatcatgt gcatgtttcc caataccgcc gatgcgctgc gcgccgcggt
    26461 cgacatgcag atcgcgcaga ccgatcgcga agacggcctt gccttgcgca tcggttttca
    26521 cgccggcgaa gtcattctgc gcgacggcga cgtgtacggc gatacggtga acgtcgcagc
    26581 gcgcatcgag gaactggcca agggcgagca gattctcacc accggcccta ccgtcgcgct
    26641 gttgccagaa tacctgaagc acggcctgcg ccgcgtcgga caagtggacg tgcgcggcaa
    26701 ggagcagccg gtcgattcct tcgaagtgat ctggcgctgg agcgcggacc tgaccatcat
    26761 ggaaaacccg gtcagccagc agccgctgcc cagcgcgcaa ctcttgttgc gctatcgcgg
    26821 gcaggaactc gagttgagcg gcgtggatcg cgagttcacc atcggccgcg aaccgggttg
    26881 cgatttggtg acgccgcaac cgcgcgcctc gcgtctgcac ggtcgcatcg agtatcgcgg
    26941 cggcgtgttc gtgctgctcg atctgtccgc caacggcacc ttcgtgcagt tcgaaggcga
    27001 tcgcgaattc gtgctgcgcc gttccttcgt gcccttgcgc ggccgcgggc gcatcggttt
    27061 gggtttgagc accacgggtg cgggcgctga cgatgcgctg gagttccgct gcgagtagtc
    27121 gttcggcgag catcgcgtcc gtccgaccgg aaattctttc tcgcccctgc acttagcgtg
    27181 ccacctcgat aagttgccgc ggcttttgca tagtcggcta tgatcgttcg cctgtcgcga
    27241 ttcccgcgca cgtcattccg cgcagcgcga cggagcgcta aagtccgcaa cagggcttcg
    27301 ctatatcgtt ccgggcagtg cgaccgctgc acaaaggttt caaacaacga gcatggcgga
    27361 cactttcttt cttcccgact ggcccatcga acccaaccag atgatttggg tcggcctgtt
    27421 gctgctgtgc ggcatcgtcg gcggcgaagc ggtgaagcgt tatgcgcggc tgccgcgcat
    27481 caccggttat acccttgcgg gcctgctgct cggacccggc gccttcggcc tgatcggcac
    27541 tcaactgctt gtcgaactgc gcgtcttcgt cgacatctcc atcggtctca tcctgttcga
    27601 actcggccag cgtctggact tgcgctggct gcggcgtacg ccggcgttgc tgttgatggg
    27661 cgtcgccgag tcggtctttg cggccctgct cgtgtttctc tgtctgcatt actacttcgc
    27721 ctattccgca ttgtcggccg ctgtcgcggg cgcgattgcc gcatccactt cgcccgcggt
    27781 gctgatgcgc gtcgcctccg agttgcgtgc agaagggcag gtgacggagc gcgcactgat
    27841 cctggccgcc atcaacagcg tgctggcctt tctcgcgctc accgtgctcg tgccctgggt
    27901 gcatctggaa tacgaaggca gtgcgttgac catcctgctg cacccgttct atctgctcgg
    27961 cgtgtcgctg ctgctcgcct tcgtcgccgc ctggatcacc ctgcgcatcg tcgtattcgt
    28021 cggcaagggg gcggaacgtc agttcgtctt cgtgatcgcg atggtggtgc tgaccgtcgg
    28081 cgcagccctc gcattgaaag tgccggtgct gctggcgttg cttgccttcg gtgcgatggt
    28141 gcgcaacttc gatcgggagc atcatttcgt cgttgtggat ttcggccgcg cgagccatct
    28201 gttctacgtc atcctgttcg tcgtcaccgg ggccatgctc gacatcggcc tgcttgccgc
    28261 agtgggtggt gcggcgcttg ctttcgtctt ggcgcgcgga ctgggcaagg cggtcggcat
    28321 cttcgcactg gcgccgctga ccggtctgtc gacacgccac gcttcgctgc tggtggtggc
    28381 gctggctccc atgtccggcc tcgcggtgat catggtgcat gagacctcgg gcatgtatcc
    28441 gcagttgagt gcgggcatcg cgcccgtggt gcttgctgcg gtcgtgatcc tcggactact
    28501 cggtccggtc gccactcagt acgcgctgcg tcgcgcgggc gaagcgagcc cggagacaag
    28561 cagaaactga cacacgatga gcgatccact cgtttttcac gaatcccagc cgctcaccct
    28621 cgggatagag ctggaactgc aactggtcaa cacgcgcgac tacaacctga cgcgcgcggc
    28681 gtccgatctg cttgccgcgc tggtcaagcg cgaacatccg ggcgagatca agcccgagat
    28741 caccgaaagc atgatcgaga tcagcaccga cgtgtgcgag acgcatgcac aggcgctgca
    28801 tgaattgaat atcctgcgcg cgctgctggc cgacactgcg gagcggctca acatcgccgt
    28861 ggcaggcggc ggtacgcatc cgttccagaa gtggagcgag cagcgcatct acgatacgcc
    28921 gcgcttcctt catctgcagg aactgtacgg ttatctcgcc aagcagttca ccgtgttcgg
    28981 ccagcacgtg catgtcggcg tgcccagcgg cgacgaggcg gtgcgctgga cgcagcttct
    29041 gtcgcgctac gtgccgcact tcatcgccct gacagcctct tcgccgtact cgctcggcgc
    29101 cgatacgcag ttcgattcct cgcggctgaa cgtcatcaac gcttttccgc tgtcgggcta
    29161 catgcccttc gtcgaggact ggaccgcctt caatgcctac tacgacacca tgcgcggcta
    29221 cggcatcgtg aaaagcatga aggatttcta ctgggacatc cggcccaagc ccgagtacgg
    29281 caccatcgag ctgcgtgtat gcgatacgcc gctcaacatc caaaccgccg cggacattgc
    29341 cgcctatgcg caggcgctgt gtgcgcactt tcacgcgcat cccgaactgc ggcaggatcg
    29401 cggattgatg ctggtataca gctacaataa gttccaggcc tgccgcttcg ggctggaggg
    29461 tacgctggtc gacccgacca gtcagcagcc ggtgccgctg gcgcaggagc tgatcgacat
    29521 gctgaccacc gtgatgccct atgccgccga gcgcggcgcg ggcgatgcga tcaagcgact
    29581 catacgcctg acggtggcgc gcgctaacga cgcgcgcaag ctgcgcgagg taatggagaa
    29641 aaaggattcg ctgaccgacg tggtgcgcga ggcgagccgg ctctggatgc agggctgacg
    29701 acggggcgaa cccacgcatt tcgagctgtg ctatgcagtt tcggctcgtg agatacgatc
    29761 tttgttgtct gccatgcagg ctgactatac aattggtcca acaatgaagc accgctcggc
    29821 ttgcgtcgcc aagggcgccg gcaatccgaa gtggaaaaag cgaggggagg cgcaatgctt
    29881 caggtgttca gcctgattat cacgctgttg ctgttctggc tgctgctgtc cggcatctat
    29941 acccccttcc ttattggcgc ggggatcgcc agcgccgttg ccgtcaccat attcgcacgg
    30001 cgcatggaaa tcgccgatcg cgaaggtcat cccatccaac tgggcctcgg tgcgctgact
    30061 tactggccct ggctggtcaa ggaaatcgtc gtttcggccc tgcagatgac gcgcatcatc
    30121 ctcgatccgc gtctgccgat cagtccgacc gtcgtgcgtt tcaaaccttc gcagaccacc
    30181 gcgccggggc tcgcgacgca tgcgaactcc atcaccctga cgcccggcac ggtgacgctg
    30241 gaggcgagtc acgacgaatt catcgtgcac ggcatcaccc gcgaaagcgc ggagggtgcg
    30301 gtcgacagcg agatggatcg ccgcgtgcac aaactggagt cgggacaatg agcatgttcg
    30361 ccgctgcagt gatcggcatt ctcgtcgcgc tgggtctggc gctcgtgcgc gcgctgcgcg
    30421 gcccgacggt gttcgatcgg gtgctcgcgg ccaacaccgt cggcacgctg gccgtgctga
    30481 tgctctcggt catcggtttt ctcaacggcc ggccggagtt cctcgacctg gccatcgtct
    30541 atgcactgct caacgtcatc ggcaccatcg cggtgctgaa gttcttcgag ttcggcgatc
    30601 tgggcgagcc cggaggagac ccgttgcgat gagcaccgtg ctagacgtac tgagctggtt
    30661 ctgcctcgcc gccggcgggc tgttctgcat catcggcgcg atcggcctgt tgcgcatgcc
    30721 ggatctgttc acccgcatgc atgcgaccag cgtgatcgaa acgctgggga ccgggttgat
    30781 tctgctgggc cttgcgctgc aggcgggctt cacgctggtg ctggccaaac tggtcattat
    30841 tgccctgctg attttcttcg ccagccccac ctcgtcgcac gcactggcga aggccgccct
    30901 ggcgcgcaag ctcgagccgc agggcctcga cgaggccggc gtcgccgcac tggaaggagc
    30961 gccgtcatcg aaaccctgat gatcaacacg gtgctgacct tcatggtggt gctcgtgatc
    31021 gggctcgtca tgcagcgcaa tctgtttgcg gtcgtggtgc tgtccggcat ctacagcttt
    31081 ctgatggcca tggtgctggt gggcctcgat gcggtcgacg tggcgatgac cgaagcggcc
    31141 gtcggcgcag gtatttccac cgtgttgctg ctcggtgcct tgtatgccac cggcgccgac
    31201 gaggcgaagc ccatgcgccg ccccttggga ccgttgctgc tcgcgatcgt gatcgttggt
    31261 gccctggtgt acggcacgct cgacctgccg gcgttctccg atccggaggc gccgatccac
    31321 acccacgtcg cgccgcgcta cctcagcgac accgtaaccg aaaccggcgt gccgaacatt
    31381 gtcaccgccg tgctggccag ttggcggggc ttcgatacgc tcggcgaagt caccgtggtc
    31441 ttcaccgccg gcatcggcgt gatcgccctg ctgcgtcgcc tgcggcgatc ggacaagagc
    31501 agggagggat agatggagcg ctatctcatc ctgcgcatcg taggcaagct gatcattccg
    31561 ttcatgctgc tgttcgggct gtacgtgcag ttccacggcg acttcggacc gggcggcggc
    31621 ttccaggcgg gcgtcatcgc ggcggcgggc gtgatctttt atgcgctcat cttcgggctg
    31681 cccgcggcgc gccggctggt gcccgaccgc ctggtggaga cgatgatcgc gctgggcgtg
    31741 ctgctgtacg ccggtgtggg cgtggcgagc atgctactcg gcggcaacta cctcgattac
    31801 ttcgtgctcg accgcgacac cgtccacggc cagcaccgcg gcatcttctg ggtggaactc
    31861 ggcgtactga tcaccgtcgc cggcgtgatg ctgaagatct tctacctgtt cgcatcgcgc
    31921 ggccgcgatg ctgcggattc gtgagcatgg aagaactcgc cagtcactac agctatttcg
    31981 tgaccatcgt gttgatggtc tgcgggctct atatcgtcat ctcgcgcggc aacctcatca
    32041 agaagctggt cgggctggcg atcttccaga cgtcggtcta tctgctctac atcgcaccgg
    32101 ggaaactgat cggcggcaca gcgccgatct acgccgaggg ttacggcgtc tactccaatc
    32161 ccctgccgca cgtactgatc ctcaccgcca tcgtggtcgg tgtggccacg ctcgcgctcg
    32221 gccttgcgct ggcggtacga gtgcgcgaag cctacggcag catcgaagag gacgagattc
    32281 tcgaagcaga cgcgcgggaa ccggagcggg tgtgatgatg gaacaactgc ccgccctgca
    32341 ggttgtcctg ccgcttatcg ctgcgccgct ggtggtgctg ctgcggcgcc cgaacattgc
    32401 ctggttcgtg gcggtggcgg cgagctgggc gagcctcgcg atcgcgctgc tgctgttcga
    32461 tcacgtcggc cagcacggcg tgatcagtta cgccatgggc agttggccgg cgccctgggg
    32521 tatcgagtat cgcgtcgata ggctgaacgc cttcgtcctg gtgctggtcg cgggcctcgc
    32581 ggcgatcgtc gcaccctaca gcctgcgcag cagtgccgcc gaagtgccgg cagagcagct
    32641 ctacctctac tacgcgctgt acctgctgtg tctggccggc ctgctcggca tcgtgattac
    32701 gggcgatgcg ttcaatctgt tcgtgttcat ggaaatctcc tcgctttcca tgtacgtgct
    32761 catcgccctc gggcgttcgc gccgcgcgca gcttgccgcc tatcagtacc tcatcatggg
    32821 cacgctgggc gcgacctttt atgtgatcgg cgtggggatg ctctatctca tgaccggcac
    32881 gctcaacatc gccgacctcg cgcagcgtgt gccggaaatc gccggtacgc gcgccgtcta
    32941 cgccgcgctc gccttcgtga ctgcgggtat ctcgctcaag ctcgcactgt tcccgctgca
    33001 cttctggctg ccgaatgcgt acgcctatgc gccctccgcg atggccgcct tcatcgccgc
    33061 gacggcgacg aaagtgtcgg tgtacgtgct gctgcgctat ttcttcttcg tgttcggcga
    33121 cgccgtccgc gccggcggtc ccgaactgag cggcatcctc atggcgctgg ccgcggtggg
    33181 tctggtcgcc gcgtcgctgg tggcctgcta tcagggcgac ctgaagcgca tgttcgcctt
    33241 ttccagcgtg gcgcagatcg gctacatcat tctcggtatc agcttcgcca accgcgaagg
    33301 cctgaccgcc gcggtggcgc atctgttcaa tcacggcatc accaagggcg cgctgtttct
    33361 cctgctcggc atggtggcgc tgcgcgccgg cggcacggat ttccgcgccg tcaacggtct
    33421 ggcgcgacgc atgccgctca ccagcatggg catcgtgctg gcgggcttca gcctcatcgg
    33481 tgtgccgggc acggcgggct tcatcaccaa gtggtatctg gtgcttggcg cgctggcgct
    33541 ggagtccatg gcgttggtgg cgctgatcgt cggctcgtcg ctcatcgcag tggtctatgt
    33601 gtggcgcttc gtcgaggcgg cttatttgcg cgaaccggcc gctgatgcgc cgggcagcga
    33661 ggcgccgctg tcgatgctga tcccggcgtg gctgctggtc gccgcctgca tctggttcgg
    33721 cttcgatacc tccttcaacg tcggcgcggc ggatgtcgca gcccgcctga tgctgaggca
    33781 gccatgacgt cgccgctgct catcgcgctg ctcattccgc tgctcggcgc cgtcggcatc
    33841 gccgcggcgc atcgcgcccc caatctgcgc gagacggtca cgctcgtcac cgccggcggg
    33901 ttgtttgtcg cggtcgcgtc tcaattgccc gcagtgctgg acggtgcgcg tcccgccgtg
    33961 tcgctgttcg aagtcttccc cggtgtgggg ctggccttcg aactcgagcc gctcggcatg
    34021 ctgttcgcac tcatcgcctc gtcgctgtgg atcgtcaatt cgatctattc gatcggctac
    34081 atgcgcggca acgacgaagg gcatcagacg cgcttctatg tctgctttgc gcttgcgctg
    34141 ttcggcgcag tcggcatcgc cttcgccggc aacctgctta cgctcttcct gttctacgaa
    34201 attcttacgc tcgtcaccta tccgctggtg acgcatcacg gcgatgcgga agcgcggcag
    34261 ggcggccgtg tctatctcgg cgtgctgatc tcgacctcca ttgcgctgat gctggtcggc
    34321 atattggtga cctacttcag cgtcggcaca ctcgacttcg aacccggcgg catcatcggc
    34381 gatcgcctga gcgacggcgc gcgcggcctg ctgctcgccc tgttcatgtt cggcatcggc
    34441 aaggcggcgt tgatgcccgt gcaccgatgg ctgccgtccg cgatggtggc gcccacgccc
    34501 gtgtctgcgt tgctgcatgc ggtcgcggtg gtgaaggcgg gcgtgttctg cgtggtgaag
    34561 gtgatcgtct atgtgttcgg catcgatctg ctggcgatga ccgcgaatgc cgcgtggctg
    34621 ccttatgtgg cgggcttcac catcatcgcg gccagtatcg tcgcactgcg tgccgataac
    34681 ctgaagcggc ggctggcgta ctccaccgtc agccagctct cctacgtcgt gctcgccgcc
    34741 gcgctgctgg cccccatttc ggtgatcggt gcgacgctgc acatcgccgc gcacgccttc
    34801 ggcaagatca cgctgttctt cgccgccggc gcgatctata cggcctcgca taagacggag
    34861 gtaagccaac tcgccggcat cgggcggcgc atgccctgga ccatggtggc tttcgccatc
    34921 ggcgtgctgt cgatgatcgg cctgccgccc accggcggct tcatcagcaa gtggttcatg
    34981 ctggccggtg cgatgcaggt ggagcattgg attgcggtgg gcgtcatcgc ggccagcacg
    35041 ctgctcaacg ccgcgtactt cgtgcccatc gtgtacaccg cgttcatgca cgcgcccacc
    35101 gatacggctg cgcatggcga agcaccgtgg ccgatggtgc tcgcgatggt ggtcaccgcg
    35161 agcctcaccg ttgcgctgtt cttctatgcg gacgtgccgc tcgccctggc gcggcaactc
    35221 gtaggagtct gaacatggcc cgccccgatc ctcgtgaacc gcactggctg acccgtccgg
    35281 acaccatacg caagttgtgg tggatcttcg gcgtcgtgct ggcgttgacg gtcgttgcgc
    35341 aattcgtcgt gccgctcgag ggccacttcg gcgcggacgg cctgttcgcc ttcaacgcct
    35401 ggttcggttt tctggcctgt gcggcgctca tcctcattgc caaggtgttg ggcgtgttga
    35461 tcaagcggcc ggaggactac taccaggtgc gcgaggaggg cgagtcgtga acctcttcca
    35521 tcccggcctc atcctcgccc tgggcggtct gctgctcggt gcgctgcctg cgcgcgcgcg
    35581 accggcggcg acgttggtgc tgccgcttct cgcattgtgg gcgttgtggg ccttgccgga
    35641 aggcatcctg tggaagggcg agtttctcgg cgtgccggtt gcgccgctcg ccgtcgatcc
    35701 gctgtcgcgc ctgttcgcca ccatctttgc gctcatggcc tttgccggcg gcttgttcgc
    35761 cttgcggcag cagagccggc tcgaagtgcc ggcggcctat atctacgcgg gcagcgccat
    35821 cggcgtcacg cttgccggcg atctggccac cgtgttcgtg ttctgggagt tgatggccgt
    35881 cggctccacg ctggtgatct ggagcggcgg cagcgccgca gcctggcatg cgagcaagcg
    35941 ctatttcgcc atccacctgc tcggcgggat gctgttgttc gccggcataa tgggccatgt
    36001 ggtgcagacg ggcgaactct atttcacccg catcgcgctc gactcgcccg cgcaatggct
    36061 gatcctcgcg ggcttcctga tcaacgccgg cgcgccgccg ctgtccgcct ggctgcccga
    36121 tgcctatccc gaggcctcgt ggagcggcat ggtgtttctg tccgccttca ccaccaagac
    36181 ggcggtgtat gtgctgctgc gcggcttccc gggcaccgag ttgctgatct gggtcggcct
    36241 gtacatggtg atgtacggca tcatctatgc gctgctggaa aacgatatgc ggcgcatcct
    36301 cgcctacagc atcgtcaacc aggtcggctt catggtcgtg ggcatcggca tcggcacgga
    36361 aatggcgctc aacggcgcgg ccgcgcatgc cttcacgcac attctctaca aggcgctcct
    36421 gctgatgtcg gcaggcgcgg tgctctacca gacgggcaag cgcaagtgca cggatctcgg
    36481 cggactgttc cgcaccatgc cgttgacctg catctgcggc atcgtcggcg cgctggcgat
    36541 ttcgtccttc ccgctgactt cgggtttcat cagcaaatcg atgatttccg aagcggccgt
    36601 ccacgagcat ctcgctttcg tgtggtacat gctcgccgcc gcatctgcgg gggtgtttct
    36661 gcacgccggg atcaagttcc cctggttcgt gttcttccag cgcgattcgg gactgcgtcc
    36721 ggcggatccg ccgtggagca tgcggctggc gatgatcctg ctcgccgcgg cgtgtatcgg
    36781 actgggtgtg ttctaccagc cgctctacgc gttgctgccg tacccggtgg attacgagcc
    36841 ctacaccgcc gcgcacgtgc tggtgtacct gcaactgctg ttgttctcgg gcttggcgtt
    36901 cttcgtgatg ctgaacttcc tgcggcgcac gctgaccatc acgctcgact tcgactggtt
    36961 ctaccgtgtg ccggccatgg cggcggtgcg catggtcgag cggttgttcg acggcgtgcg
    37021 tcgcggcgtc aacgcaacgg catcggccac gagttcccgg ctcaaccggc tgctcgaccg
    37081 ctggatcggc agcgaaggcg tgctcgcgcg taccatttct accggaggca tggccttctg
    37141 gctggcggcc atcctggccg gctatctggt gacggcctac tggtagtcgg tttgcggggg
    37201 aagggcgcct aaagggtcag gccgcgatag ccgatggcgc gtgaaatgcg gtcggcggtg
    37261 tccttcacca gttcggccca ttcgggcttg aggcgctcgg ccggtgcgga aatggacagc
    37321 cccgccacga gcgcgccggt atcgtcgcgg atgccggcgc cgatgcagcg cacgcccagc
    37381 tccgcctctt cgttatcgta ggcgatgtgc gtcttgcgca ctttgtccag ttccttttcc
    37441 agcgcctcca gcgtgccgat gctgttgcgc gtaaaggtcg gcaggccggt gcgctcggcg
    37501 tagccccgga tgccttcggg gccgtcctcc agcaggaaca gcttccccac ggcggtgatg
    37561 tgcagcggcg catgttcgcc gatgatgttc accacgcgca tcaactgccg gccgctggag
    37621 gagcgctcga tataaacgat ttcgtcgtcg cgccgcacgg acaggttcac cgcctcgttt
    37681 gtctgcgcat gcagttcgcg catgagcggc agcgcctgtt cgcgcacgct gatgcgcgac
    37741 ttcaccaggt tgcccagttc cagcaggcgc atgccgaggc gatagctgcc ctgatcgacg
    37801 cgctccacca tatggtcgtt gaccatggcg gtgaggatgc ggtgggcagt ggagggatgc
    37861 agctcggcgc ttgccgccag caccttgagg ctgacggcat taggcgactg cgccaggaca
    37921 tcgagcagct tcatcatgcg cttgatgacc tggatggagt tcttcgattc cttctcggtg
    37981 ccgctcatga aaaggctctg cctgaggttg tttggacaag gcgttcgcct gtggtcgacc
    38041 aactgcccgg ctgaaattct acaccgcatg ccgaaaatgc cggcggcgcc ggaccaagca
    38101 taacgcccaa ccgcgtccaa ggcacgccgg gccgttcaca gccgcgtgct accatgatcc
    38161 gccttgtctt cgaccgccgt acgcgtctgt gaaagtaggc ctgttcgtca cctgtctcgt
    38221 cgacctgatg cggggagggc tttggttccg gcataacggg ctgcgcccat tagcctccac
    38281 cgaagcctcc ctggccgcat cgacatcgct gtctgccgga gcggcgcgat gaaagtaggc
    38341 ctcttcgtca cttgtctcgt cgacctgatg cggccgtcga tcggctttgc cgccctgacg
    38401 ctcctggagg cggccggctg cgaggtggtg gtgccgatga cgcaaagctg ctgcggccag
    38461 cccaactaca acgccggcga tcgcaagtcc gcacgccgca tggcggccaa ggtgctcgac
    38521 gaattcgagg gcgtcgatta catcgtggcg ccgtccggct cctgcgccgg gatgatccgc
    38581 acgcattacc ccgacctgtt ccgcgacgac gatccggcgc tgcgcgcgcg cgtcgatgcg
    38641 ctgtgtgcgc gcacttacga actcaccgat tttctggtca atgtcgccaa actgacaaag
    38701 gtgcccggcc gctacgaggg cacggtgacc tatcacgact cctgttccgg tctgcgcgaa
    38761 ctgggcgtca agcagcagcc gcgcgcattg ctcgcgcgcg tgcccggtct ccggctcacg
    38821 gaaatggacg aatgcgaaca gtgctgcggt ttcggcggcg cctttgccgt caagttcggc
    38881 gagatcagca cggagatcgt cgagcgcaag tgcaaggcca tcgacgccag cggtgcggac
    38941 gcggtggtgc tgggcgatct cggctgcatg ctcaacatcg agggccggtt gcgccggcgc
    39001 ggcgacgagc gcacgcaagt gtttcatgtc gccgaagtgc tggcgaacga ccggaagccg
    39061 gaactagaat gagcgccgcg cttgggcgtg gccagggcgc tgcgattaca accaccacac
    39121 tgagggggca gcatggcaga ggacgcaggc aagctggtgc ttcgtgtgac gctcggcgta
    39181 ctggtgctgt tgcacggtat cgccaaggtc gttaacggcc cgtcgcatat cctgggcatg
    39241 atggaccaaa tcggcatgcc ggaggtcttc ggctacgccg tctatatcgg cgaagtgctc
    39301 gcgcccatac tcgtgatcat cggtctgttc accattcccg ccgcgttgat catcatggtg
    39361 aacatgatct tcgccatcgt gctggcgcac agtcatcaac tgttcagcct caccaagagc
    39421 ggcggatggg cgctggaact gcagggcatg ttcctgttcg gcgcgttggc cgtggcattg
    39481 ctgggcgccg gccgttacag cgccggaggg cgatacggaa agtggaattg acgcaagccg
    39541 ccgatcaggc cgcgaccgtg acgctgcgcg aagtggtcat cgccaacttc aacgaggtca
    39601 tccgcctgca gccggcgccg ggacaggaga atttcatcgc gcccaacgtg ctgtcgatcg
    39661 cgcagtcgaa ggtggcgccg aatttcgcgc ccgtcgccat ccatgccggc gacgatgtgg
    39721 tcggcttcct catgttcggg caggacatcg agacgctgaa gtggcacatc gtgcggctca
    39781 tgatcggcgc gcaataccag cggcgcggct atgcgcgcac ggcgctgcgt cagttgatcg
    39841 atcagttggc gcagcggccg gactgcgagg agatcatcat cagcttcatg ccgggcaacg
    39901 tcgcggcaga gaagctgtat gagagcctcg gcttcgaaaa gaccggcgag ttcgacgacg
    39961 aaggcgaagt gctgatgcgt ctggaattga aagactgatg ccggctatga cgatgaacgc
    40021 tgatcgtcat tccggccaag cccgcagggc gcgagccgga atccaggcaa agaaccggat
    40081 tcccgctttc gcgggagtga cgaagccggt gtcctcttag cgcatgcaaa tccgctccat
    40141 ccatttcaag gcgcaagcct cccgctcgct tgccaatccg gtgctgcagg ccaacctgca
    40201 caagttcggc atcggcggcc tggcgctgct gcgcgctcgc gcggtggagg cctacggcag
    40261 caaagccttc gacaccctgc gcgacgcagg cgaggcgatc cgtaatcgca ccctggccga
    40321 actggatgtg tggatcgagc gcttcgaggc ggaagccacc cgccgcggcg cgaaggtgct
    40381 ttacgcgcgt gacgacaagg aagcgcagca gctcgtgctg gagatctgtc gccggcacga
    40441 ggtgaagaag gcgatcaagt cgaagtcgat ggtgtcggag gaggtcggcc tcaacgaagc
    40501 gctgatcgcc aacggcgtga cgcccatcga aaccgatctg ggcgaataca tcatccaact
    40561 cgccggcgaa ccgccttcgc acatcattgc cccggcggtg cataagagca aagaggaagt
    40621 ctccgagctg ttcgccgcca agcacggcac gccgcccaag accgacaacg aggcgctcac
    40681 gcgcgaggcg cgcgccgaac tgcgccagca ctttctcacc gcggacctgg gaatttccgg
    40741 cgcgaacttc ctggttgcgg aaaccggctc cggcctgatc gtgaccaacg aaggcaacgg
    40801 ccgcatggtc accacgctgc cgcgcgtgca tgtgagcatc accggcatcg aaaaagtggt
    40861 gccgacgctg gaagacgtat ccaccctgtt gcgcctcctg ccgcgctcgg cgacggggca
    40921 gccgatctcg aattacgtgt ccttcttcac cgggcccaag ggcgcgcagg acgcggacgg
    40981 tcccgagcac atgtacttca ttctggtgga caacggccgc acgggcttcc tcggcacgga
    41041 catgcaggag atgctgcgct gcatccgctg cggcgcgtgt atgaatcatt gtcccgttta
    41101 ccgcactgtc ggcggtcatg cctacggctg ggtgtatccg gggccgatgg gctcgatcct
    41161 tacgccgagc tatgccggca tggagaacgc gcccgatctg ccgcacgcct ccacgctatg
    41221 caaccagtgc ggcgtggtct gcccggtgag gattccgctg cccgatctgc tgcgcaagat
    41281 gcgcgagaag caggtgcaga ggcgcatgcg ccccttcggc gaaatgttcg cgctgcgcct
    41341 gtgggcctac gccgcgctgc atccgcggct ttatgggctg gcgcagtcct tcatcggcgg
    41401 cttcctgcgt ctgaaggcgg gacgcggcaa gcgcatcaac agtctgccct tcctcggcgc
    41461 ggcgtggacg caggggcgtg attttccgcg cccgcgcaat cagaccttca agcagaaata
    41521 cagccgctac ccgggcaagc ggacgcagta acgcgtgctc aggcttcctc gccgagcgcc
    41581 gcggcgtccg gctgatggcc cgcgatgtcg gcgagcgacg gaccgcggcg cttggcgcgg
    41641 tacatcgccg catcggcgct gatgatcagc gcttcggccg tgtcgccgtc ctccggatag
    41701 caggcgatgc ccaggctcgc gctcacggac aattcgtgtt cgcgatagga gaagggccgc
    41761 gccacttcct gcaggatggc gtcgccgatg gattgcgcct cctgtacgtc gttcagcgac
    41821 tccagtacca ccacgaattc gtcgccgccg acgcgtgcga ccgtatccgc atcgcgcacg
    41881 cagcgcttca gccggcgcgc cgtctccacc agtgccgcgt cccccgcgct gtgcccgtag
    41941 cggtcgttga tgggcttgaa gccgtccagg tcgatgacca gcacagcgag cgccttggcg
    42001 tcgcggcggc gctgcgcgag cgcgtggttg atgcggtcgt tcagcacata acggttgagc
    42061 aggccggtca gcgcatcgtg ttgcgactgg tgcagcagat ggcgctcgtt ctcgcgcagc
    42121 cgccggttgg cctcggccag ttccgccgtc cgcgcctgca ccttcgcttc cagttcggtt
    42181 tcggacattt tcaatgccgc gaccagcgcg cgttcggctt ccagcgcctc ggactgcgcc
    42241 cgcgtgcgtt cggcgcgcat gcggttcatg cgctgcgcca tggccagagc gaacaggaac
    42301 acctgtagcg cggtggtgat ctccagcgcg tgatcggtga acgggttgcc cggcagcagg
    42361 ccgatggagc gtgcggcgaa agtggcgacg ccgatttcga acacggtcca ggcgacgatg
    42421 gcgaaccggt cctgtgtgta gccccgccgc aggcaataga acaccaccgg cattgcggcg
    42481 cagggggtga ggatgccgac cagcgacagc acgatcgccg ccggctgata gaagcccgcg
    42541 gccatgatca gcgcgcagca cccgtaggcg atggccgagc cgtagatgac gtagtcgaag
    42601 gcgcgcaccg gccggttcag cgccagcagt tcgcgcgtga agagcatgga cagcagcccg
    42661 gcgctgctca tcccgtacac caccgccgga ttgctccatt gcgggaatgc gggccacagc
    42721 agcagcgggc cgatgccgtg cagcgaggcc tggccgatcg atagtcctag acagaacagc
    42781 gcgaacagca ggcagatgcg ctcgcggacc agcagataga gcagcaggtt gtacagcatc
    42841 atcaccacca gcagcccgaa gtaggcgccg accagaatgg tgtcgatctg cgagtgcgcg
    42901 atgaaattct cggtggtgcg cagcgtggcg tgcaggttga tggtgccttc ggtgctgacg
    42961 cgtagataga aggtatgcgg ctcgctcgac gtctccagcg gaaagaccag attgcggtgc
    43021 gcgatcggcc gcgcgtgggg cgcgacggaa tcgcccgcgc gcatcagggt gtagccgccg
    43081 tctccggtcg gtgcgtagaa ctgcacctcg tccatacgcg agtagggcag gtcgagcagc
    43141 cacaggcgcg gtgtctcgct ttgcagcgtg aaacggaacc agtaagcgga atcggaatag
    43201 ctgcgcacga tatcgcgccc gtcgtgcgca gccggcgcga aggacgcatc gaggcgaagg
    43261 acgtcatcga ctcgcagcgt gccgcccgga tcttccaatg catcgacgtg gggggtcaga
    43321 tcgtaatggc tctgcccgtc cgacacgctc agcgtcgccc ccgcgacagc cgggccgaag
    43381 gcgcttaata aagcggcgag cgccaccgcc gcatacagct tccccaacag caataactcc
    43441 ccgaaatagt gcgatcgtgg gcttctcgcg gccgcactga tcaacctctg tcggtatatc
    43501 ggcagcaggc ctcgcactct tgagggcggg gccggaaata tttggattcg cccggcacgc
    43561 ggattccata tcgcagcgca tgcccgatca cctgcagcga taccgacaga agcgcgattt
    43621 tcgcatcacc agcgagccgc agggcggtgc ggcggcggga gaaggcttgt ccttcgtcgt
    43681 gcagaagcat gccgcccggc gcctgcatta cgacttccgc ctcgagttgg acggcgtgct
    43741 gaagagttgg gccgtgccca aaggaccgag tctcgacccg gccgacaagc gcatggccgt
    43801 gcatgtggaa gaccatccgc tcgactacgg cggcttcgag ggcgtgattc cgcccggcca
    43861 gtacggcgcc ggcacggtga tcgtctggga tcgcggcatc tgggagccgg tgggcgatcc
    43921 gcacgagggc tatcgccagg gcaaactcaa gttcgatctg tacggcgaaa agctccaggg
    43981 ccgctggacg ctggtgcgca tgcacggccg cgccggcgag aagcaggagc cgtggctgct
    44041 gatcaaggag cgggacgagg ccgcacgccc ggcgtcggaa tacaacatcg tcgaggaact
    44101 gcccgacagc gtgctgggtg ccggaagcaa ggcgaagaag ccggcgcgaa agaaaaccgc
    44161 caaacgcgca cgcagcaaac tcgcgctgcc ggcgcaggcg cgcaaggcgg aactgccgct
    44221 cacgctgtcg cccatgcttg caacgttggt ggacgaaccg cccaagcgcg gcgagtgggt
    44281 gtacgaagtg aagttcgacg gctatcgcct gctggcgcgc gtggaaggcg gcgacatcgc
    44341 gctgttcacg cgcaacggtc acgactggac cagcaagctc aagagcctcg cgcgtgccat
    44401 cgacgcgctg cagctacccg actgctggct ggacggcgaa atcgtggtgc agaacgacca
    44461 gggcgtgccc gacttccagg cattgcagaa cgccttcgac acttcgcgca cgcaggacat
    44521 cctgtactac gtcttcgatc tgccctattg cgacggtcac gatttgcgcg ccgtgccgct
    44581 gcgcgagcgc cgcgcgctat tgcaacgact cctgcaggca catccgcaat cgcgcgtgcg
    44641 ctacagcgag gatttcaacg caccgcccga agaactgctc gatgcaatgt gtcgcctgcg
    44701 catggaaggc ctgatcggca agaaggccga ctccgcctac acctcgcggc gctcgcccgc
    44761 gtggatcaag ttgaaatgca tgcagcggca ggagttcgtg atcggcggct ataccgaccc
    44821 gcaaggcagc cgcatcggat tcggctcgct gctgctcggc ctctacgacg agcagggcag
    44881 gctgcattac gccggcaacg tcggcacggg cttcaacgac aagctgctgc aaagtctgtc
    44941 cgccagactg gaagccttgc gcaccgacga aatgcctttc gcggacaagc cgacgcgcgg
    45001 cgcgggcttc ggtgccggcg gcggcaaacc gcattgggtg aagcccaagc tggtcgcgga
    45061 agtaagcttc ggcgaatgga cgcgcgacgg ccgcatccgc catccggtct tccatggcct
    45121 gcgcacggac aagccggcga aggcgatcac cagggagaag cccatgcatc tcgaagaggc
    45181 gcctgcgaag cgcaaggccg cgacgaaaac gcgcgccgcc gccaaactgc ccgccggatt
    45241 gaaagtcacc aatcccgagc gcgtcatcga tccgtccacg ggcatcacca aactggaact
    45301 ggtgcagtac tacgccaggg tggcaccgct gatcttgccg catctcaaag ggcggccggt
    45361 ctcgctcgtg cgcgcaccga gcggcatcga gggacagcac atcttccaga aacacggcgc
    45421 acccataccg ggcattacgc acctggaccc caagtacgac cgcgaccacg atccgttgat
    45481 ggaaatcgca agcgccgaag cgctgctcaa cgccgcacag cagaactgcg tcgagtacca
    45541 cacctggaac agcaccacca aggcgatcga tcagccggac cggatgacct tcgatctcga
    45601 tccgggtgaa ggactgaaat ggccgcagat gcaggagggc gcgctgctcg tgcacggctt
    45661 cctcgaagaa ctcggactgc gcagcttcct caagaccagc ggcggcaagg ggctgcatgt
    45721 catcgtgccg ctcaagccgg gtccggatta cgagacggtg aaggagtttt ccaaagtcat
    45781 cgtcgaacat cttgcgcgcc atgtgccgca acgtttcgtc gccaagagcg gcccgaagaa
    45841 tcgcgtcggt aagattttcg cggactacct gcgcaacggt cgtggcgcga cgacagtcgc
    45901 ggcgtggtcg gcgcgtgcgc gtccgggcat gggcgtgtcg gtgccggtcg aatgggacga
    45961 gttgccgcag ttacgcagcg gcgatcactg gaccatacgc acgatcgacg agcggctcga
    46021 gctcgatgcg tggcgcgagt acggcaaggc acagcggcag acactcaccc gcgccgccaa
    46081 ggcgctcgac ttcgaacttc ctccggcgaa agccgcaccc gccaagaagc gcaagcgtgg
    46141 cgcttgagtt gtccgaacgg acaacgccaa atatttgacg tcgactccgc gcacacatgt
    46201 tttgacacgt acaatggtcg acaccattcc agtgtcggac gcgatgcgcg tcagcgcacc
    46261 ggccgccgct cattcaacgc aagccttatc cggcttgcgc gcatttcagg agccgcccta
    46321 tgccgatcaa ctccgcgcca tgcaaaacct gccgcaacat ccagggggca gccgaccgca
    46381 acgatgccgt ggtgcccgag atccgcgaga gcgacacggt catccgcggt ggtgcacgct
    46441 tttatgtgtg ccccgactgc gaaaaacttt acgccgtgaa ggaaagcaat gaaggcggaa
    46501 cggtgcacta cagcatcgtc accgccgctt cggtcaatcg tcgctaccag tggatggtgg
    46561 acggtgccga cggcacgcgc gagcgcgcca aaggcgccat gacggaagag gaagcgaaga
    46621 ggaagtacag caacccggaa aagatcatcc accggccgat ctatcaaccg ccgaacaatt
    46681 gatcgtcatt ccgggcagcg g
//
LOCUS       DATGOU010000070         9598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_622955, whole genome shotgun sequence.
ACCESSION   DATGOU010000070 DATGOU010000000
VERSION     DATGOU010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9598
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622955"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..189)
                     /locus_tag="VKZ48_04120"
     tRNA            complement(100..189)
                     /locus_tag="VKZ48_04120"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(153..155),aa:Ser,seq:cga)
     gene            complement(436..840)
                     /locus_tag="VKZ48_04125"
     CDS             complement(436..840)
                     /locus_tag="VKZ48_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77077.1"
                     /translation="MKRTQTLAAIAAAFGMLSGAHAADGNTRAPADTPQDRASDRRQS
                     LDDHMADERRRQEQREEAQGGTAGAYEGAIGGTPRQANPRTGEFGSGGAQTGDLPASR
                     GGADNVTGRKNPEAAQGDAAGKEKKRNVPEYP"
     gene            complement(931..2205)
                     /gene="serS"
                     /locus_tag="VKZ48_04130"
     CDS             complement(931..2205)
                     /gene="serS"
                     /locus_tag="VKZ48_04130"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HLU77078.1"
                     /translation="MLDPQLLRTDPDRVAKRLADRPFELDVAAFRTLEDERKQVQSRT
                     QELQAQRNALAKQIGQAKGKGEDASALMAQSNAANSELGELEKRLADVQARVQAFMAA
                     IPNIPHESVPTGRSADDNVEVRRVGEPRKFDFEVKDHVDVGAALGGLDFETATKIAGS
                     RFALMTGGVARLHRAITQFMLDLHSGEHGYTEVYVPYMVNKDSMYGTGQLPKFEEDLF
                     AIPRGEEKFYLIPTAEVPVTNIVRDEIVPLDKLPLKFVCHSPCFRSEAGSYGKDTRGM
                     IRQHQFDKVEIVQIVHPDRSWEAHEELTANAEEVLKRLELPYRVVALCTGDMGFASAK
                     TYDLEVWLPAQNAYREISSCSNFEAFQARRMQARFRNEKGKPELVHTLNGSGLAVGRT
                     LVAILENYQNADGSVTVPAVLKSYMGGLEVLR"
     gene            complement(2217..2588)
                     /locus_tag="VKZ48_04135"
     CDS             complement(2217..2588)
                     /locus_tag="VKZ48_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77079.1"
                     /translation="MSDFYIDPGSDLVLVSVFAADFAIYWVFWRIARMANTIRNQEVI
                     APRLFACLGVGILTALFIWYTRANLIDPAIYDALSPREQGEYLGGLLRTAVFPALIII
                     GISAARAWRDAQERKYRGKNI"
     gene            complement(2595..3908)
                     /locus_tag="VKZ48_04140"
     CDS             complement(2595..3908)
                     /locus_tag="VKZ48_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017525946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HLU77080.1"
                     /translation="MADLFAAPAPAAPLAEALRPRTLDDVVGQRHLLGAGKPLRLAFE
                     SGKPHSMILWGPPGVGKTTLARLMADAFDAEFVALSAVFSGVKDIREAVQRAKDVLAQ
                     TGRRTILFVDEVHRFNKAQQDAFLPYVEQGLFTFIGATTENPSFEVNSALLSRAAVYV
                     LQSLSAAELTELFDRANATALQMEFDEQARERIVGMADGDARRLLNVLEILRTAAEAA
                     GVAKIDGDFVASTMASNLRRFDKGGEAFYDQISALHKSVRGSNPDAALYWLVRMLDGG
                     ADPLYLGRRIVRMASEDIGLADPRALRLALDACEAYERLGSPEGELALAQAVVYLACA
                     AKSNALYVAYNAARAFVGQDESRPVPVHLRNAPTRLMKELGYGRAYRYAHDEPEAYAA
                     GENYFPEGMPPTRWYDPTDRGLEARIAEKLAYLRSLDERAKSSRE"
     gene            complement(3925..4575)
                     /gene="lolA"
                     /locus_tag="VKZ48_04145"
     CDS             complement(3925..4575)
                     /gene="lolA"
                     /locus_tag="VKZ48_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HLU77081.1"
                     /translation="MNRRCSRALAGRLAKFLVLLSFFAATGATAGGVERLQQFMSRTQ
                     TAQGEFAQRIYDHSGRVTQESSGRFAFSRPGKFRWTYEKPYAQLIVGDGARIWIYDED
                     LQQVTVRKLDQALGSTPAALLAGSNEALKGFELKDEGVREGLEWVLAVPREQESNFSR
                     IRMGFSEAGLAAMELADSFGQTTVLTFDKLRRNPALDPELFKFTPPEGADLIGAIE"
     gene            complement(4578..6869)
                     /locus_tag="VKZ48_04150"
     CDS             complement(4578..6869)
                     /locus_tag="VKZ48_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HLU77082.1"
                     /translation="MKARAAASAPAAPPLPGKIARLLRESRWFALVAVAIFLALILGT
                     YERADPGWSHSVEVERIANGGGIVGAWLADLLLYLFGVSAWWWVLLLIYTVIWGYRRL
                     DGSSISDRRPFFIAFGGFMLVLLASASLEALRFHSVPAALPLAPGGMIGTAISGAAQG
                     ALGFTGGTLLLMAMFAIGLSLFTGISWISLMEAVGAGLEWMWRSAIDAWHRRQDRKAG
                     EHATLRRGRVVEAEKKRIEEHAPIRIETPVIEIPKSERVIKEKQVPLFHDLPDTPLPP
                     LVLLDEPPRDVEVMSPDTLEFTSRLIERKLNDFGVEVQVLAAYPGPVITRYEVEPATG
                     VKGSQIVNLVKDLARALSVVSIRVVETIPGKSCMGLEIPNPKRQIVRLSEILGSKVYH
                     DMGSPLAIALGKDIGGNPVVADLARMPHLLVAGTTGSGKSVAINAMILSLLYKSEPRD
                     VRLIMVDPKMLELSVYQDIPHLLAPVVTDMKQASSALMWCVGEMEHRYKLMSHMGVRN
                     LSGFNAKIAEADKKGESIPDPFALTAEDAVPLTKLPHIVVVIDELADLMMVVGKKVEE
                     LIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRIAFQVSSKVDSRTILDQMG
                     AETLLGQGDMLYLPPGTGLPQRVHGAFVADDEVHKVVDWLKQQGEPDYIEGLLETGEA
                     AAGGDLVAGGEAGGGEADPLYDQAVEIVLRTRRPSISLVQRHLRIGYNRAARLIEQME
                     IAGLVSPMQSNGNREVIVPARAE"
     gene            complement(6977..7660)
                     /locus_tag="VKZ48_04155"
     CDS             complement(6977..7660)
                     /locus_tag="VKZ48_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005673207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HLU77083.1"
                     /translation="MAGPASVSTLLLRNVPLFAGLPEGQLALLTRVVGRKAFPRSTTI
                     ISAGDPTDALYIVISGRLKVMMSDDEGREVILAILSQGEFFGEMGLIDESPRSATVIA
                     IEPCELLSISKADFKKCLQENFDISMAVMRGLVKRLREADKKIGSLALMDVYGRVARL
                     LMEMAEDVDGERVVTKKLPKQDIAKMIGASREMVSRVMKDLQTSGHIEVRGGAIYLRE
                     GMLVSEARA"
     gene            7836..8798
                     /gene="trxB"
                     /locus_tag="VKZ48_04160"
     CDS             7836..8798
                     /gene="trxB"
                     /locus_tag="VKZ48_04160"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HLU77084.1"
                     /translation="MSNKPVKHHRLIILGAGPAGYSAAVYAARANLKPVLITGMAPGG
                     QLMTTTDVDNWPADFAGVQGPELMERFQKHAERFETELVYDHINRVELRERPFRLEGD
                     SAVYTCDALIIATGASAQYLGLPSEQAFMGKGVSACATCDGFFYRGQDVAVIGGGNTA
                     VEEALYLSNIARHVTLVHRRDKLRAEKILQQKLLDKANVSIEWNHVLDEVLGDDAGVT
                     GMRIKHVQTGETKEIPVHGVFIAIGHTPNTGIFEGQLEMEGGYIVTRGGRHGMATATS
                     VPGVFAAGDVQDHVYRQAVTSAGTGCMAALDAEKYLDGLESEAA"
     gene            8807..9346
                     /locus_tag="VKZ48_04165"
     CDS             8807..9346
                     /locus_tag="VKZ48_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HLU77085.1"
                     /translation="MGKDEFDNELRLFREAVRDAVPLRVEPRHRPVPRKPAPVPVQRL
                     LDDREALRASQEADFDDDEPPETGQSESYLAPGIGRDVLRKLRRGAWRVERELDLHGL
                     TKEGARVEVAAFLQECVKQRWRCVRIVHGKGLGSRDRVPVLKGRVRVWLTKRAEVLAY
                     CEPPEARGGGGALLVLLKG"
     gene            complement(9432..>9598)
                     /locus_tag="VKZ48_04170"
     CDS             complement(9432..>9598)
                     /locus_tag="VKZ48_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HLU77086.1"
                     /translation="VKIEVVVTDEMLDPAIEAIIKAARTGKIGDGKIFVMDVEQVVRI
                     RTGESNEAAV"
BASE COUNT         1709 a         3101 c         3071 g         1717 t
ORIGIN      
        1 tcgtacgtcc caaaacgcat gcgttttggt cgaacccggg agacttctcg cccttcccgt
       61 tcgccaaata aaaatgcccc acgagggggc aatttcattt ggcggagagg gagggattcg
      121 aaccctcgat agagttgccc ctattccggt tttcgagacc ggcccgttca accactccgg
      181 cacctctcct taatcggtac tgctactgct cgtttgcgca acctattccg gttttggctc
      241 cggccgcgct gcgcggttaa cttgccaagg caagttagcc tccgccgcaa tgtcggggta
      301 ctggatcccg acattgtcga gaccggcccg ttcaaccact ccggcacctc cgtgtctcgc
      361 tcgcgcagca gcatcgcgcg aaggcgcgca ttctaccgga acatggggac ttcgcccacc
      421 ctcggcgcgc aagcgtcacg gatactcggg cacgttgcgc ttcttttctt tgccggccgc
      481 atcgccttgc gcggcctcgg gatttttccg acctgtgacg ttatccgcgc caccgcggct
      541 ggcaggcaaa tcgcctgtct gtgcaccgcc gctgccgaat tcgccggtgc gagggttggc
      601 ctgacgcggc gtgccgccga ttgcgccttc gtatgcgcct gccgtaccgc cctgcgcttc
      661 ctcgcgctgc tcctgccggc gcctttcatc ggccatgtga tcgtccagcg actggcgacg
      721 gtccgaagca cgatcctgcg gcgtgtcggc cggtgcgcgg gtgttgccgt ccgcggcatg
      781 cgcaccggaa agcatgccga aagcggcggc aatggccgct aaggtctgag tgcgtttcat
      841 acgctgtctc ctcgctgaaa cctcagactc tatgcaagcc ttcctgcaat cgctgtagga
      901 cgagagcgcc ttgcgctgta ggggagacgc tcagcgaagg acttccagtc cgcccatata
      961 ggatttcaac acggccggca cggtgacgct gccgtcagcg ttctgatagt tctcgagaat
     1021 cgcgaccagc gtgcggccca ccgccagacc ggacccgttg agcgtatgca cgagctcggg
     1081 cttgcctttt tcgttgcgga aacgcgcctg catgcgacgc gcctggaagg cttcgaagtt
     1141 ggagcaggag gaaatctcgc gataggcgtt ctgcgccggc agccagactt ccagatcgta
     1201 ggttttggcc gaggcgaagc ccatgtcgcc ggtgcacagc gccacgacgc gataaggcag
     1261 ttccagacgc ttcagcacct cctcggcatt cgcggtgagt tcctcgtgcg cttcccagga
     1321 acggtcggga tgcacgatct gcacgatctc gaccttgtcg aactggtgct gacggatcat
     1381 gccgcgcgtg tccttgccgt aggagccggc ctcgctgcgg aagcacggcg aatggcagac
     1441 gaacttcaaa ggcagcttgt cgagcggaac gatttcatcg cgcacgatgt tcgtcaccgg
     1501 cacttcggcg gtcgggatga gatagaactt ctcctcgccg cgcgggatcg cgaacaagtc
     1561 ctcttcgaac ttgggcaact ggcccgtacc gtacatgctg tccttgttga ccatgtacgg
     1621 cacatagact tccgtgtagc cgtgctcgcc ggagtgcagg tcgagcatga actgcgtgat
     1681 cgcgcgatgc agccgcgcca caccgcccgt catgagggca aagcggctgc cggcgatttt
     1741 ggtggcggtt tcgaagtcga gcccgcccag cgccgcgccc acgtccacgt gatcctttac
     1801 ttcgaagtcg aacttgcgcg gctcgcccac gcggcgcact tcgacgttgt cgtccgccga
     1861 gcggccggtc ggcacgcttt cgtgaggaat gttcggaatg gcggccatga aagcctgcac
     1921 gcgcgcctgc acatcggcga ggcgcttttc cagttcgccc agttcggaat tggccgcgtt
     1981 cgattgcgcc atcagcgccg aggcgtcctc gcccttgcct ttcgcctgcc cgatctgttt
     2041 cgccagcgca ttacgttgcg cctgcagttc ctgcgtacgg gattggacct gcttgcgctc
     2101 gtcttccagc gtgcggaagg cagcgacatc gagttcaaag gggcgatcgg cgaggcgctt
     2161 ggcgacgcgg tcggggtcgg tgcggaggag ttgcggatcg agcatgggga aggccgttaa
     2221 atgtttttgc cgcggtattt gcgctcctgc gcatcgcgcc aggcgcgcgc ggcggaaatg
     2281 ccgatgatga tcagcgccgg aaagacggcg gtgcgcagca atccgccgag gtactcaccc
     2341 tgctcccgcg gcgagagggc gtcgtatatc gccggatcga tcaggttggc gcgggtgtac
     2401 cagatgaaca gcgccgtgag aatgcccaca cccaggcagg cgaacagccg cggggcgatc
     2461 acttcctgat tgcggatggt gttggccatt ctggcgatgc gccagaacac ccaatagatg
     2521 gcgaagtcgg cggcgaaaac gctcaccagc accagatcgc tgcctggatc gatataaaaa
     2581 tcggacatga atatctactc tcgagacgac ttcgcgcgtt catccagact gcgcaaatag
     2641 gcgagcttct cggcgattct cgcttccagc ccgcggtcgg tcgggtcgta ccagcgcgtc
     2701 ggcggcatgc cctcggggaa ataattttct ccggcggcat aggcctccgg ctcatcgtgc
     2761 gcgtagcggt aggcgcgacc gtagccgagt tccttcatca aacgggtcgg cgcattgcgc
     2821 agatgcacgg gcaccggacg cgattcgtcc tgaccgacga acgcacgcgc cgcattataa
     2881 gcgacgtaca gggcgttcga tttggcggcg caggcgagat agaccaccgc ctgcgccagc
     2941 gcgagttcgc cctcggggct gccgagccgt tcataggctt cgcaggcgtc gagcgccaga
     3001 cgcagcgctc gcgggtcggc caggccgatg tcctcgctcg ccatgcgaac gatgcgccgg
     3061 cccagataaa gcggatcggc gccaccgtcg agcatgcgca ccagccagta cagcgccgca
     3121 tcggggttgg agccgcgcac cgacttgtgc agtgcggaaa tctggtcgta gaaggcttcg
     3181 ccgcccttgt cgaagcggcg caggttgctc gccatggtgc ttgcaacgaa gtcgccgtcg
     3241 atctttgcca cgcccgcggc ctcggcagcg gtgcgcagga tctccagcac gttgagcagc
     3301 cggcgcgcat cgccgtcggc catgccgacg atgcgctcgc gcgcctgctc gtcgaattcc
     3361 atttgcagcg ccgtcgcatt cgcacgatcg aaaagctcgg tcagttcggc cgcgctgagg
     3421 ctttgcagca cgtagaccgc ggcccgcgac agcaacgcgc tgttcacctc gaacgaggga
     3481 ttttcagtgg tcgcgccgat gaaggtgaac agtccctgct cgacgtaagg aaggaatgcg
     3541 tcctgctgcg ccttgttgaa gcgatggact tcatcgacga acaggatcgt gcgccggccc
     3601 gtttgcgcca gcacgtcctt cgcgcgctgc accgcttcgc ggatgtcctt caccccggag
     3661 aacacggccg acagcgcaac gaactcggcg tcgaaggcgt cggccatgag gcgcgcgagc
     3721 gtggtcttgc ccacgcccgg cggcccccaa aggatcattg aatgcggctt ccccgactcg
     3781 aacgcaagac gcaagggctt gccggcgccg agcaaatggc gctgaccgac gacgtcgtcg
     3841 agcgttcggg gtcgcagcgc ttcggccagc ggagcggccg gcgccggtgc ggcaaagaga
     3901 tcggccacgg tcggaccttg atgcctactc gatggcgccg atgaggtcgg cgccctcggg
     3961 cggcgtaaac ttgaacaact cgggatcgag cgcgggattg cgacgcagct tgtcgaacgt
     4021 cagcaccgtg gtctggccga agctgtcagc cagttccatg gccgcaaggc cggcttcgct
     4081 gaaacccatg cggatgcgcg agaagttgct ttcctgttct cgcggcacgg ccagcaccca
     4141 ttcgagccct tcgcgcacgc cctcgtcctt caactcgaag cccttgagcg cctcgttgct
     4201 gccggcgagc agcgcggccg gcgtggagcc gagcgcctgg tcgagcttgc gcaccgtcac
     4261 ctgctgcagg tcctcgtcgt agatccagat gcgcgcaccg tcgccgacga tgagttgcgc
     4321 gtaaggcttc tcgtaagtcc agcggaactt gcccggtcgc gaaaaggcga aacgcccgct
     4381 cgattcctgc gtgacgcggc cgctgtgatc gtagatacgt tgcgcgaatt cgccctgcgc
     4441 agtctgcgta cggctcatga actgctgcag gcgctcgacg ccgcccgctg tggcgccggt
     4501 ggcggcgaag aaagaaagca ggacgaggaa tttcgcgagt cgccccgcga gggcacggct
     4561 gcagcgtcgg ttcatgacta ctccgcgcgg gcgggcacga tgacttcccg gttgccgttg
     4621 gattgcatcg gcgacaccag gcccgcgatc tccatttgtt cgataagccg cgccgcgcgg
     4681 ttgtagccga tgcgcaagtg ccgctgcacc agcgaaatcg acggccggcg cgtacgcagc
     4741 acgatttcca cggcctgatc gtacagcgga tcggcctcgc cgccaccggc ttcgccgcct
     4801 gcgacaaggt cgccacccgc cgccgcttcg ccggtttcga gcagaccttc gatgtaatcg
     4861 ggctcgccct gctgcttgag ccagtccacc accttgtgca cttcatcgtc ggccacgaac
     4921 gcaccgtgca cgcgctgcgg cagaccggtg ccgggcggca ggtagagcat gtcgccctgc
     4981 cccagcagcg tttcggcgcc catctggtcg aggatggtgc gcgaatccac cttcgacgat
     5041 acctggaagg cgatgcgtgt gggaatgttc gccttgatca gcccggtgat cacgtctacc
     5101 gacggccgct gcgtggcgag gatgaggtga atccccgcgg cgcgtgcctt ctgcgccagg
     5161 cgtgcgatca attcctcgac cttcttgccg accaccatca tcaggtcggc cagttcgtcg
     5221 atcaccacca cgatatgcgg cagcttggtg agcggcaccg cgtcttcggc ggtcaacgca
     5281 aagggatcgg gaatgctctc gcccttcttg tcggcctcgg cgatcttggc gttgaaaccg
     5341 gacaggttgc gcacgcccat gtgggacatg agcttgtagc ggtgctccat ctcgcccacg
     5401 caccacatca gcgcggacga agcctgcttc atgtccgtga ccaccggcgc gagcagatgc
     5461 ggaatgtcct gatagaccga cagctccagc attttcgggt cgaccatgat caggcgcacg
     5521 tcgcgcggct cggacttgta aagcagcgac aggatcatcg cgttgatcgc gacggatttg
     5581 cccgaaccgg tcgtgccggc caccagcagg tgcggcattc tggcgagatc cgcgaccacc
     5641 ggattgccgc cgatgtcctt gcccagggca atcgcgagtg gcgagcccat gtcgtgatag
     5701 accttggagc ccagtatttc ggacaggcgc acgatctgcc gcttgggatt gggaatctcc
     5761 aggcccatgc acgacttgcc gggaatggtc tccaccacgc ggatgctcac caccgacagc
     5821 gcacgcgcaa ggtccttcac cagattgacg atctggctgc ctttcacgcc cgtggcgggt
     5881 tcgacctcgt agcgcgtgat gaccgggccg ggataggcgg cgagcacctg cacctccacg
     5941 ccgaaatcgt tgagcttgcg ttcgatcagg cgcgaggtga actcgagcgt atccgggctc
     6001 attacttcca cgtcgcgcgg cggctcgtcc agcagcacca gcggcggcag cggcgtgtcc
     6061 ggcaggtcgt ggaacagcgg cacctgcttt tccttgatca cgcgctcgga cttgggaatc
     6121 tcgatgaccg gcgtttcgat gcggatgggc gcgtgctcct cgatgcgctt cttctccgcc
     6181 tcgaccacgc ggccgcggcg cagggtggcg tgctcgccgg ccttgcggtc ctgacggcga
     6241 tgccaggcgt cgatggcgct gcgccacatc cactccaggc cggcgccgac cgcctccatc
     6301 agcgaaatcc acgaaatgcc ggtgaacagc gacagcccga tggcgaacat cgccatgagc
     6361 agcagcgtcc cgccggtgaa accgagcgca ccctgcgccg caccgctgat tgccgtgccg
     6421 atcatgccac cgggtgcaag cggcagcgcc gccggcacgc tgtgaaagcg cagcgcctcc
     6481 aggctggcgg aggcgagcaa cacgagcatg aagccgccga aggcgatgaa gaacggtcgc
     6541 cggtcggaaa tggaagagcc gtccaggcgg cggtaacccc agatcacggt gtagatgagc
     6601 agcagcaccc accaccacgc cgagacgccg aacagataga gcagcagatc cgccagccac
     6661 gcgccgacga tgccgccgcc gttggcgatg cgctcgactt cgacgctatg cgaccagccc
     6721 ggatcggcgc gttcgtaggt gccgaggatg agcgcgagga agatggcgac cgccaccagc
     6781 gcgaaccagc gcgattcgcg caacaggcgg gcgatcttgc cgggcaacgg cggcgcggcg
     6841 ggcgccgaag cggcggcgcg tgctttcaag ggcttggaca aatgaggcag taccgcggaa
     6901 gcgcggcggg caaagcggca atactagaac agccggacac caagcgtgaa cccttcaggg
     6961 gcggcgtccg gcgcgctcag gcgcgcgcct cggacaccag cattccctcg cgcagataga
     7021 tcgcaccgcc gcgcacttcg atgtggccgc tggtttgcag gtccttcatg acgcggctga
     7081 ccatctcgcg ggaggcaccg atcatcttgg cgatgtcctg cttgggcagc ttcttggtga
     7141 cgacgcgctc gccgtcgacg tcttccgcca tttccatcag caggcgtgcg acgcggccgt
     7201 acacatccat cagcgccagg ctgccgatct tcttgtcggc ctcgcgcaga cgcttcacca
     7261 ggccgcgcat gacggccatg gagatatcga aattctcctg caggcatttc ttgaaatcgg
     7321 ccttggaaat gctgagcagt tcgcaaggct cgatggcgat cacggtggcc gaacgcggcg
     7381 actcgtcgat gaggcccatt tcgccgaaga actcaccctg ggaaagaatg gccagaatga
     7441 cttcgcgacc ttcatcgtcg ctcatcatga ctttcaaccg gcccgaaatc acgatgtaca
     7501 gcgcatcggt cgggtctccg gcggaaataa tggtcgtgct gcgcgggaag gccttgcgcc
     7561 ccaccacgcg ggtgagcaaa gcaagctgtc cttccggcag accggcgaac aaaggcacgt
     7621 tgcgtaggag cagtgtcgag acgctagctg gaccggccat tggcttcccc ctccgtggat
     7681 attcgattta tcgacgtttt gtaatgtcgt tggcgaagaa tatagcagat atgcttgagc
     7741 gcgcgtggtt ttttggcaac agccccctcg gggaaaggcc cttgccacgg cggctataat
     7801 ttatttccca gccatcatgc ctcaatgcca acgtcatgtc gaacaagccc gttaagcacc
     7861 accgtctcat cattctcggc gccggaccgg ccggatacag cgcggccgta tatgccgccc
     7921 gcgccaacct caaaccggtg ctgatcacgg gcatggcgcc gggcggccag ctcatgacca
     7981 ccaccgacgt ggataactgg ccggccgact ttgccggcgt acaggggccg gaattgatgg
     8041 agcgtttcca gaagcacgcc gagcgcttcg agaccgaact ggtctacgac cacatcaacc
     8101 gcgtcgaact gcgcgagcgc cccttccggc tggaagggga cagcgccgtt tacacttgcg
     8161 atgcgctgat catcgccacc ggcgcgtctg cgcaatatct gggcctgcct tcggagcagg
     8221 cgttcatggg caagggcgta tcggcctgcg ccacgtgcga cgggttcttc tatcgcgggc
     8281 aggacgtcgc cgtcatcggc ggcggcaaca cggcagtgga ggaagcgctc tatctgtcga
     8341 acatcgcccg acacgtcacg ctggtacatc ggcgcgacaa gctgcgcgcg gaaaagattt
     8401 tgcagcagaa gctgttggac aaggccaatg tgagcatcga gtggaatcac gtgctcgacg
     8461 aagtgctggg cgacgatgcc ggcgtgaccg gcatgcgcat caagcatgtc cagaccggcg
     8521 agaccaagga gataccggtg catggcgtat tcattgccat cggccacacg ccgaacaccg
     8581 gcattttcga agggcaactg gagatggaag gcggttacat cgtcacccgg ggcggacgcc
     8641 acggcatggc taccgcgacc agtgtgccgg gtgtgttcgc cgcgggcgat gtgcaggatc
     8701 acgtctaccg ccaggccgtc accagcgcgg gcaccggctg catggccgcg ctcgacgccg
     8761 agaaatatct cgacggcctg gaaagcgagg cggcgtagga ggccccttgg gcaaggacga
     8821 attcgacaac gagctgagac tgttccgcga agcggtacgc gatgccgtgc cgcttcgcgt
     8881 cgagccgcgt catcgtcccg taccgcgcaa gccggcgccg gtgccggtgc agcggctgct
     8941 cgacgaccgg gaagccttgc gcgcgtcgca ggaagccgac ttcgacgacg acgaaccacc
     9001 cgaaaccggg cagagcgaaa gctatctggc gcccggcatc ggccgcgacg tgctgcgcaa
     9061 actgcgccgc ggcgcatggc gggtggaacg cgaactggac ctgcacggat tgaccaagga
     9121 aggcgcgcgg gtggaagtcg ccgccttcct gcaggaatgc gtcaaacagc gctggcgctg
     9181 cgtgcgcatc gtgcacggca agggattggg ctcgagggat cgcgtaccgg tcctcaaagg
     9241 ccgggtgcgc gtctggctca cgaaacgcgc cgaagtgctg gcctactgcg aaccgcccga
     9301 ggcgcggggc ggcggcggcg cgctgctggt gttgctgaag gggtagtcga gagtcgtgat
     9361 tcgagagtta agagtggaga gccaactccg ccgctcccga ctcacgactc tccactctcg
     9421 actgctttgg cttagaccgc cgcctcgttg ctttcgccgg tgcggatgcg caccacctgc
     9481 tccacatcca tgacgaaaat cttgccgtcg ccgatcttgc ccgtgcgtgc ggccttgatg
     9541 atcgcttcga tcgccggatc gagcatttcg tcggtgacga cgacttcgat tttcacct
//
LOCUS       DATGOU010000071        36041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3741466, whole genome shotgun sequence.
ACCESSION   DATGOU010000071 DATGOU010000000
VERSION     DATGOU010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 36041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36041
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3741466"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="VKZ48_04175"
     CDS             complement(<1..1099)
                     /locus_tag="VKZ48_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012351922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HLU77087.1"
                     /translation="MSAPIVVYVPMDSAAISVGADQVAVRIIIEATAQKKDVLVKRNS
                     SRGMLWLEPLVEVQVGDKRIAYGPVTPDDVPGLFQAGFLEGNEHALRIGAPEEHPYLK
                     NQERLTFARAGITEPTSIGDYIEHGGYRGLKNALAMSPADVVKAVTDSGLRGRGGAAF
                     PTGIKWNTVLQQQADQKYVVCNADEGDSGTFSDRMIMEGDPFVLIEGMTIAGIACGAT
                     QGYIYLRSEYPYAHAALNRAIEVAKAKGYLGDDVLGSGKKFELEVRMGAGAYICGEET
                     SLLESLEGKRGEVRYKPPIPAIEGLFGKPTVINNVISLASVPIILDKGAEYYRDYGMG
                     RSRGTLPIQLTGNVKHGGIVEKAFGVTVRELL"
     gene            complement(1096..1563)
                     /locus_tag="VKZ48_04180"
     CDS             complement(1096..1563)
                     /locus_tag="VKZ48_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HLU77088.1"
                     /translation="MNTIPSPAEAEKLYADLLQKPGALLPILHAIQDQLGFIPSDYVS
                     SIAKSLNLSRAEVHGVISFYHEFRTTPAGKHVIQVCQAEACQSMGVEALTRHAEEKLG
                     TKLKGTSADGKYTLEPVYCLGNCACSPAMMVDGRLHGRVTAQRFDEVVGELQA"
     gene            1745..2878
                     /locus_tag="VKZ48_04185"
     CDS             1745..2878
                     /locus_tag="VKZ48_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLU77089.1"
                     /translation="MFKILIKPQWLIRGDEAEMRQALPRLLELLALLHEDGNLARACR
                     ELDMSYRYAWGMIRDGGELFGAPLVETAKGQGTKLTELGQRLLWAEKRINARLAPVLD
                     SLASELEVEIQQTLSRHDTALRIHASHGFAVETLRRFLSERNIPLELKYRGSGEALAS
                     LCRDTCEVAAFHVPTGDLQERALGQFMSWLKPNSQRLIHLATRRQGIMVAHGNPLRII
                     ATSDLLKPDVRFVNRQPGSGTRVLLDLLLENERLDGREIQGYDTSEYTHAAVAAYIAS
                     GMADAGFGVETAARKFNLDFVPVVTERYFLICNTASLELPAVKEMLGILRNNEFRAAV
                     NELRGYDATECGKMMTLEEAFPEFAAMEKPERRPVKKKGAAAA"
     gene            complement(3061..3759)
                     /locus_tag="VKZ48_04190"
     CDS             complement(3061..3759)
                     /locus_tag="VKZ48_04190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HLU77090.1"
                     /translation="MKFVENPLNEAELDELSALLEELPSADRMNLETLDGYFAALLAG
                     PEHPPASEYLAVVYGLQPGDETPPFTDATKSRRIAELLQRHWQTIGAQLFSLLEKDEV
                     YVPIMAEDEQGSALGNDWAVGFLKGTEQRYALWEELYEDDDESLLTPMLMLANEHHPD
                     PDLRTPPLSEGRRASLLDDMAWNVTLINRYYADHRLTGGKPREPFRREGEKVGRNDPC
                     PCGSGKKYKHCCAG"
     gene            complement(3814..4518)
                     /locus_tag="VKZ48_04195"
     CDS             complement(3814..4518)
                     /locus_tag="VKZ48_04195"
                     /inference="COORDINATES: protein motif:HMM:NF015642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HLU77091.1"
                     /translation="MQASTFPSIGQTALRQLGELLASLPSGRAMNVPTLDGFFAALAA
                     GPDVVPESEYLPVVCGIADAEDLQALERFDGVESLVALLAQLMEGIRAALDAGLRENS
                     VYLPNFDTDANGRALGNDWANGFLRGLRLRGKSWSALLNDPREVHLMVPIFTLAHEHN
                     ADAQLRAAPISDAERGDLIRRATAYLTLIHRYFEPLRANANAARERGRVGRNDPCPCG
                     SGKKYKQCCGNPQHLH"
     gene            complement(4642..7416)
                     /locus_tag="VKZ48_04200"
     CDS             complement(4642..7416)
                     /locus_tag="VKZ48_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HLU77092.1"
                     /translation="MLKTLKGRTALAAAGLATIGIITVTNLHSDLSEHTVLASGVAQH
                     ISYTQRVAVDIDRQLRLARAALSEFAANIDRSHFHNPITLHRFLNMAVGLQERFEAVS
                     VFDAEGRLLASRPATEVRSVHEEAWFRDTIANNGVPRIHRPFLSPLSRKPVTLLTYPV
                     HDESGRLQAVVVGAFALTQDELWIAKPSTDMRGESAFIVVTDDRYIGLHADIGLVGQP
                     LDALGKVAQTIERGMAKEVASVIDEDHRGVSGLYAFREISSAGWTLVGVGTNEAAYAS
                     LAELSNRMLLAGAVLAMLLFPLMWLLVSRMLQPLDHLRSEMRRMRTGESGAPEVHAMR
                     ATTELSDLANEFAQVAAAWRTAEGALQREKERAEVIVESIGEGVIATDRHGVIAAMNG
                     AAESIIGWRDEEVVGRPFVEVFVVKDEKHGVDLGDIAQLAMQQSSSACIPNALLRLRN
                     GEYIPIHNSAAPIRNAEGEIDGAVIVFRNIAAERAAAQQLEWRATHDAMTGLVNRHAY
                     EQALRRLCASVRPHESHALLVLDLDQFKLVNDTCGHAAGDALLKELADLFVRRSRRSD
                     LVARLGGDEFAVVMYGCTSEQAVRLAETLLKAVAGHRFEWDGRLFAVGASIGVVEINA
                     DFCDPSEVVRAADMAAYMAKRSGGTRICNHSGSDEALNAMRLEMAAVSTIRAAIEADR
                     LQLYAQAIHPVAHDGDGLYFEILVRMLDENGALLPPAVFMPAAERYGLMRQIDRWVIE
                     HAARALAENFGPDGWHRLDTASINLSAITLKDGEIGAYILEHLERHGIPFERICFEIT
                     ESAAIENLAAARELMQTLRRRGLRFALDDFGVGMTSLSQLRDLPIDVLKIDGSFICGI
                     DRDDVNGPLVDAIQTIARKFSMTTVAERVETPAELAYLRRLGIEYAQGYLLARPVPLQ
                     QMIDSVATTV"
     gene            7636..8604
                     /locus_tag="VKZ48_04205"
     CDS             7636..8604
                     /locus_tag="VKZ48_04205"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HLU77093.1"
                     /translation="MTRRKCLVAALLLWLGMAAPCLAAYPSQPITLIVPFPPGGTSDA
                     VAHALRTRLAENLKQQVTIDYVAGGAGAVGAMRLKHAAPDGHTIMVASLTTYVAGPLS
                     NTAVNYDSARDFDLLTVAVHVPNVLVVHPDVPAQTVAEFVDYLKRHPDQVRFSTSGAG
                     GQDQLSMQLFWQRTGTRGVFVHRKGSAPAVADVAAGRVQASIQNLSVALPYIENAQLR
                     ALAVTGSDRSSAIVSTPTLTELGVPGIETYSWQGIAAPKGLPPAVKTRLHKALVASLD
                     APEVRRHLTRAGFEVVANTPAEFERFLHAQRHHWQAIIDRARRTSP"
     gene            8669..9028
                     /locus_tag="VKZ48_04210"
     CDS             8669..9028
                     /locus_tag="VKZ48_04210"
                     /inference="COORDINATES: protein motif:HMM:NF015729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HLU77094.1"
                     /translation="MYVSQLLGVLLLTAIYLAGAQLAAALDSAIPGSIYGLLGCAAAM
                     LAMPRATQCLRPGAALMLVLVPLFLVPITVRMAVRIDFGNPATWRAIAVLGFACLIGV
                     AATGIFARLLLRKEAGR"
     gene            9025..9711
                     /locus_tag="VKZ48_04215"
     CDS             9025..9711
                     /locus_tag="VKZ48_04215"
                     /inference="COORDINATES: protein motif:HMM:NF016088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HLU77095.1"
                     /translation="MSVLAALALSVLGYVAGVWLQRRTGSKPYMQPILLGMFVVVAVL
                     ALLDAPATEDYLAHNGLLLDLLMVAVVAFAIPLVDNVRVVLRDLIGLAFVVLAAGAFI
                     ASSTLALCYVAGIDAASIAAFSLRSVTNPIAIAVAEQNAISVDLAMLGVFITGFVSVV
                     VVEPILHRMGITDPRHLGLVLGITAHALGIARALEISSLAAAYATVGMICTGLLYAFA
                     IPSLVRLLPL"
     gene            complement(9718..10167)
                     /locus_tag="VKZ48_04220"
     CDS             complement(9718..10167)
                     /locus_tag="VKZ48_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLU77096.1"
                     /translation="MQIGQICNPVVVVARKEMSVADAARLMCEENVGSLVVVEDGVEG
                     RKVTGILTDRDIVLSVVARDLDARTVKVGEVMEANPVSVTTTHSVADALHRMRQHGVR
                     RIPVLSPNGVLEGIVTVDDVLAIIAEELNDLVSTINRQQEREGAARK"
     gene            complement(10280..11455)
                     /locus_tag="VKZ48_04225"
     CDS             complement(10280..11455)
                     /locus_tag="VKZ48_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HLU77097.1"
                     /translation="MSRPDIPMPRSESFHGRRLRFSRTEFAGAFGDIGTLIPFLAAYI
                     VVAGVPAMGILLSFGVALIAVGLYYRTPFPVQPMKAAGAVVATQAGQLAITAQVVYCA
                     SMLTGFVWLLLAVTGLAKRITALVSRPVVLGIVLGLGIGFMLEGARMITDGWLVGAIA
                     LFGALLLLENRYVPVMFLLLLFGAAVALFQDPAMLEELRELRPGFVWPEFALRDTTWS
                     DWSVALLFLVLPQLPLTLGNAVIAITEENNRLFPDRPVTEQRVSRSTGLMNIFGGLFG
                     GVPMCHGAGGLAGHIRFGARTGGAPVMFGVLLLVLALFFSASIDVLLRLFPVPVLGAI
                     LFLTGAQLALGSCDFSRDKGERFVTVTTTALAIWNVGVAFLFGLIAAAFLKRGWLKF"
     gene            complement(11505..12302)
                     /locus_tag="VKZ48_04230"
     CDS             complement(11505..12302)
                     /locus_tag="VKZ48_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HLU77098.1"
                     /translation="MKPRLIVALVAAMAFTSAVNAEEAVRLYAAGSLKAVMTDIGAAY
                     TKAHGVAVKGEFGPSGLLRKRLAEGEPADVFASANMAHPQALHKAGKSGPVTMFARNR
                     LCALTSPKVEVTTETLLERMLDPQVKLGMSTPKADPSGDYAMALYDKAEALEPGAAKA
                     LSDKALRLTGGPDSPPPPKDRVVYGVLVDEGEADIFLTYCTNALLAQKEVPSLKIVQV
                     PEALAVGADYGLTVASDRAEAHKFADYILSDKGQALLKQHGFAPPAP"
     gene            complement(12299..13135)
                     /locus_tag="VKZ48_04235"
     CDS             complement(12299..13135)
                     /locus_tag="VKZ48_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLU77099.1"
                     /translation="MDLKRRKLFAVAIAAAAMLSLPAQAQDKFITVASTTSTENSGLF
                     KHILPIFEKETGIQVRVIAQGTGQALDTARRGDADVVFVHARAAEEKFISEGHGVKRH
                     PVMYNDFVLIGPKSDSAKVKGMKDITAALQKIKYANTPFVSRGDKSGTHMAEVKLWNV
                     AGIDIAKDKGAWYRETGQGMGPALNAAASMNGYILADRGTWISFKNRGDLEILVEGDK
                     RLFNQYGVILVNPEKHSHVKKDAGQKFIDWIISDDGQKAIAGYKIGGEQLFFPNANES
                     GV"
     gene            complement(13126..13866)
                     /locus_tag="VKZ48_04240"
     CDS             complement(13126..13866)
                     /locus_tag="VKZ48_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLU77100.1"
                     /translation="MDKQPPASLSPSILPLALEEVSFAVNGHTIIDRISVVFDAGPRT
                     IIVGPNGAGKSVFMRLCHGLLKPTGGIVRWNVETGARGRQAMVLQRPVMLRRSALANI
                     EYALKLQGVSGPERRERALEALEVVGLRDLAERSARVMSGGEQQRVALARAWALRPEV
                     LFLDEPTASLDPTASAEVERIVQTIHAAGTKIVMSTHNLGQARRLGDEVLFIYGGRLV
                     ERAPVDQFFKQPATAEAAAFLKGELPWT"
     gene            complement(13859..14563)
                     /locus_tag="VKZ48_04245"
     CDS             complement(13859..14563)
                     /locus_tag="VKZ48_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008253011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLU77101.1"
                     /translation="MNEISQAFGQAVALIAQFDQTLLEIVLLSLRVSLTAVLIATLIG
                     LPLGATIAVARFPGRNAVIVGLNALMGLPPVVAGLLIYLLLSRAGPLGEFGLLFTPAA
                     MIIAQAFLITPIVAALARQAIEDAWIEYRDQLRSLGASNLSASFTLLWDTRFSLLTAV
                     LAGFGRAAAEVGAVMIVGGNIDGVTRVMTTAIALETSKGDLPLALGLGVILMTLVITL
                     NAAVYLTKEAAQRRYG"
     gene            complement(14890..15183)
                     /locus_tag="VKZ48_04250"
     CDS             complement(14890..15183)
                     /locus_tag="VKZ48_04250"
                     /inference="COORDINATES: protein motif:HMM:NF015861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HLU77102.1"
                     /translation="MFEDDVHIAGILVHARPELIERVQENIQSLDGAEVLTATQSGKI
                     VVTLEAESSSGISETMAAIGDVYGVVSTALVYEHHEAAEIRLEDYNAGAVQLH"
     gene            complement(15267..16775)
                     /locus_tag="VKZ48_04255"
     CDS             complement(15267..16775)
                     /locus_tag="VKZ48_04255"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011549402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HLU77103.1"
                     /translation="MATIKQDDFIQSIADAFQFISYYHPEDFIRAMSAAYEREESPAA
                     KDAMTQILVNSRMCAEGHRPICQDTGIAMVFLKIGMDVRWDANMSVEDMVNEGVRRAY
                     LAPDNVLRASVLAEPAFGRKNTRDNTPAVIHMSVVPGDKVEVTVAAKGGGSENKAKFK
                     MLVPADDLVEFILQQVPEMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMDPIDMQELK
                     ARGPRNKTEELRIELHDRINALGIGAQGLGGLTTVLDVKVREYPTHAASKPVAIIPNC
                     AATRHAHFVLDGSGPAQLQPPSLDLWPKLDYAAPEGARRVNLDAITREEIASWKSGDR
                     LLLSGKLLTGRDAAHRRLTEMLARGEKLPVDFRNRFIYYVGPVDPVRDEAVGPAGPTT
                     STRMDKYTEAMLAQTGLIGMVGKAERGPAAIEAIKRHKAVYCIAVGGAAYLVSKAIRT
                     ARVLAFEDLGMEAIYEFEVRDMPVTVAVDAEGVSVHDSGPRQWRREIPIRAA"
     gene            complement(16837..19497)
                     /locus_tag="VKZ48_04260"
     CDS             complement(16837..19497)
                     /locus_tag="VKZ48_04260"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLU77104.1"
                     /translation="MHGSTGHRAIEAKAQRFALLAGRLAPPWLVLLLSLFVTLVVWQL
                     VATQNRESAKLRFDYEVQQAVQGIDERINLHASVLRGARGLLAGSAFVTQADWQRYLQ
                     DINVEQGYEGLDAVGLALRVAEADKALYLEAMRALGREDYDIQPPGERPFYFPVTYYD
                     SASMPNSMAVGFDMWSDPMRRAAMRRAARAGRLAVTSTIPLLNGGVGQRGFTMYLPVY
                     AGGGVPETVDQREARLTAFVFSSYLMSQFLDGLFGPGSAKLLTLRVYDGIRRLPETLL
                     YDSGVYFESLRPDEPPRFEHLRTIEVGGAEWTLHFAATDAFDRALDRGDEVAVWLIGG
                     PISLILFIMMRALAASRMSAIDAADRAAERMRASQAHFTAAADNAYDAIVSADQQGNI
                     IYFNPAAERLYGYRAEEAIGQSLMMLASPELQATYAERYAQWMQRPPGGDDQNVHEAI
                     AVRKDGSRVPVEITTARWQTETGDFVTIVVRDVTVRKQAEEETRRMSSELERRVSERT
                     AELQRTLEEYERADQFRRSVMESAVVGLVTFDPRGRITSANKVFCKLLGYGEDELRGQ
                     HWSLLTSPADAERLTPELKRIVTERESIINREVELIRQDGRVVCVAFGTNPLIVRGEV
                     LGSVAAVMDITERKHAEARMRTFNAELERRVAERTAELKTANEEMESFTYSVSHDLRA
                     PLRHIDGHVQMLLEEFRGELDAAKRQRLERITSAALHMAQLIDDLLRFSRMGKSALHK
                     APVEMGHLVARVCQSLEEEKQAPAVEWRIGELPTVECDEHLMTQVWANLLSNALKYSS
                     TRDVAVITVEARREADHWIFSVSDNGVGFDMRYADKLFKVFERLHTGDFPGTGIGLAT
                     VQRIVSRHGGRCWAEGRPDEGATFYFSLPA"
     gene            complement(19755..20273)
                     /locus_tag="VKZ48_04265"
     CDS             complement(19755..20273)
                     /locus_tag="VKZ48_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HLU77105.1"
                     /translation="MRTGWTIAVAALALTACATAQRDVPTARAELVPTAGNQAKGTVN
                     FAQYRDKVRVSGKITGLVPNSQHGFHVHEKGDCSAPDATSAGGHFNPLNKAHGHAVPG
                     GRHAGDMPNLRADGAGQATFSFDLSGMSVNEGETNVVGRSVIVHAKPDDYRTQPTGDA
                     GARLACGVIEKR"
     gene            complement(20316..20573)
                     /locus_tag="VKZ48_04270"
     CDS             complement(20316..20573)
                     /locus_tag="VKZ48_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77106.1"
                     /translation="MRAVILFMMLSFAMPVLAKLPPPTPEEAAAAAAKKAKADEAAKQ
                     AAELLAKAQDRAVENYRRNQQAGGGATPNASTSSAQGQPAQ"
     gene            complement(20590..21654)
                     /locus_tag="VKZ48_04275"
     CDS             complement(20590..21654)
                     /locus_tag="VKZ48_04275"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HLU77107.1"
                     /translation="MRQQTIGWILCCGLALMLLASGGAWAQADRTAEQVQRQTTQPFN
                     NAPYWRGVRSGEAGVSTVQGRETGVLIQSEGETWRQVRNGPLTQISGWALALAFVGAL
                     VFFAFKGGIGLREPETGRRIVRFTLWERVLHWTTAISFLILAFTGLAILFGRYVLVPI
                     MGHSAFSFIAAGAKLLHNFVGPLFIVCTLAMFFTFVKDNIWRDYDREWLRRFGGLFSR
                     GHNEPKSHRFNAGEKAWFWGGLSLLGLVVGISGLVLDFPNFDQTRQTMQTANMVHLIG
                     ATLFMVGAIGHIYMGTIGMKGAFNAMATGEVDETWAKEHHYYWYEDVKAGRIPSAGVP
                     PTVTPEVPPSARGPAPGTQT"
     gene            complement(21799..22017)
                     /locus_tag="VKZ48_04280"
     CDS             complement(21799..22017)
                     /locus_tag="VKZ48_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77108.1"
                     /translation="MRTLMIAVASALVLAGCSEPDQSATYNETDRSYSGKTDSRPWEA
                     APYDGDQAKWERELAQRAANLNEYSRTR"
     gene            complement(22026..22658)
                     /gene="fdh3B"
                     /locus_tag="VKZ48_04285"
     CDS             complement(22026..22658)
                     /gene="fdh3B"
                     /locus_tag="VKZ48_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase FDH3 subunit beta"
                     /protein_id="HLU77109.1"
                     /translation="MARMKFLCDAERCIECNGCVTACKNVNEVPWGVNRRRVVTMNQG
                     VPGEKSISVACMHCSDAPCMAVCPVDCFYQTSEGVVLHDKDMCIGCGYCFYACPFGAP
                     QFPQTGAFGMRGKMDKCTFCAGGPEQTGSAEEFAKYGSNRLAEGKLPACAEMCSTKAL
                     IGGDGDVLSDIFRQRVTMRGKGVEMVGWQTAYGRPEKTAQAADAGRETVK"
     gene            complement(22723..25677)
                     /locus_tag="VKZ48_04290"
     CDS             complement(22723..25677)
                     /locus_tag="VKZ48_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HLU77110.1"
                     /translation="MLLTKRSGTATTAGARAGIAHGLAAALTETIDRRTFLKRSGVTV
                     GAGALATQLPFNVITEAKAQDKPANAPKVEVKRTVCTHCSVGCAIDAVVENGVWVRQE
                     PVFDSPLNLGAHCAKGASVREHGMTDHSHRLKYPMKLVNGKYQRVSWDEALDAISKKM
                     LQIREESGPDAMYYIGSSKHNNEQAYLMCKWVRLWGTNNTDHQARICHSTTVSGVANT
                     WGYGAMTNSYNDMHNSKAMFYIGSNAAEAHPVSMLHSLHAKESGAKIIVCDPRFTRTA
                     AKADQYIRFRSGTDVPVLFGLLWHIFNNGWEDKEYINARVYGMDKVREEVLKTWTPAE
                     VERVSGVPEAALYKAAETMAKNRPSTLVWCMGQTQHSIGNAMVRASCIVQLALGNIGV
                     PGGGANIFRGHDNVQGATDIGPNPDSLPGYYGVSPGAFKHWAEVWNVDLDWLTKRYVP
                     GMMNKPGITVSRWCDGVLEKNEYIDQGPNIRGVFYWGHAPNSQSRGLDFKRAMDRLDL
                     LVVVDPYPSATAAMAAMPGNKEDHNPNREVYLLPAATQFETSGSVTASNRSIQWREKV
                     IDPVFEAKPDHTIMYLLAKKLGFADELVGKRNGKQNIQVVNDEPVIEDITLEFNRGSW
                     TIGYTGQSPDRLKAHMRNMHVFDVKTLRAKGGVDKETGYVLDGDYFGLPWPCYGTAAI
                     KHPGSPVLYNNSLPMMDGGGCFRANFGVEREGVNLLAEDGSHPKGDEITTGYPEFDHV
                     LLKKLGWWDDLTPEEKQAAEGKNWKTDLSGGIQRVAMKHGSHPWGNAKARAVVWNFPD
                     PVPLHREPIYSPRPDLVDDYPTHDDKKAFWRLPTLYKTVQQKNRDISKKFPIVLTSGR
                     IVEYEGGGEETRSNPWLAELQQNMYVEINPRAANDRGIRNGEYVWVSTPEGARLKVMA
                     KVTERVDANLAFMPFAFSGWWLGKDLLEYYPEGAAPIVRGEAANTATTYGYDSVTMMQ
                     ETKTTVCQIEKA"
     gene            complement(25689..25880)
                     /locus_tag="VKZ48_04295"
     CDS             complement(25689..25880)
                     /locus_tag="VKZ48_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77111.1"
                     /translation="MAKPKLSRRKFMLSVGAGTAAAAAAAVAGNAASAATSKQEQAPE
                     RKGYELSEHVRKYYRTTLV"
     gene            complement(25904..26530)
                     /locus_tag="VKZ48_04300"
     CDS             complement(25904..26530)
                     /locus_tag="VKZ48_04300"
                     /inference="COORDINATES: protein motif:HMM:NF014651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HLU77112.1"
                     /translation="MNTGLDRVQNLNLNPEAQARADWYAFLARIWYAPPDEALLRVMA
                     DAPASGDEAAAPLDRAWAQLARAARETDLQSAKLEYDLVFIGTGKAEVTPYMAHWLPG
                     TTGKDRQLLRLRNLLDEWGLARAESAHEPEDHLAALCEVMRHLILAGGAEENSISRQA
                     ELFNTYLAPGYRGFVAAARDSQQTHFYKSVALCTEAFFSIEEQSFQML"
     gene            complement(26535..28610)
                     /locus_tag="VKZ48_04305"
     CDS             complement(26535..28610)
                     /locus_tag="VKZ48_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HLU77113.1"
                     /translation="MQQVHRLKLCNCNGTMRLDPEALRSALRTESPLTVHDQLCRREI
                     GAFQKDAGSDCLVACTQEARLFAEVADAAESKADLRFVNIRESAGWSVEGEQATPKIA
                     ALLAAAQLPAPEPVPNVAYQSAGRTLIVGPAVAALPWAEQLADQLDVSVLLTDAQGAE
                     LPVERRYAIWSGEVRRLSGYLGAFKLKWQPRNPIDLDLCTRCNACIAACPEQAIDYSY
                     QIDLNKCTGHRECVKACGAVRAIDFDRAPTVRTADFDLVFDLNAAPLIKIPHKPQGYF
                     APGGDAIAQGRAAAQLAHLVGEFDKPKFFVLDERICAHSRSKKTGCSNCMDVCSTWAI
                     SSKGDSIQVEPHLCAGCGGCTTVCPTGAIRYAYPSVSDTGARLKTMLSVYRAAGGKAA
                     AILFHDGSTGRDHIGRLARQGRGLPARIIPMEQWHPASIGIDLLLGAIAYGAHQVMLL
                     LSPQQMDEYGEALERQLAYAQSILHGLGYEGRHFGLLRAEGVPALEQALWALEPAAGV
                     AQTATFNLANNKRNALDFIFEHLLKHAPQPAEEIALAAGAPFGRVNVNKDKCTLCLAC
                     VGACPSSALVDGRDTPALRFIERNCVQCGLCVNTCPEDALSLTPRLVLADYAKKEVTL
                     NEAQPFHCIRCHTPFGTKQVVETMLGRLTNHSMFVDGPALRRLQMCADCRVIDMMEAA
                     ENAEMAVKE"
     gene            complement(28717..29358)
                     /locus_tag="VKZ48_04310"
     CDS             complement(28717..29358)
                     /locus_tag="VKZ48_04310"
                     /inference="COORDINATES: protein motif:HMM:NF023176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3306 domain-containing protein"
                     /protein_id="HLU77114.1"
                     /translation="MSDEEHFLSRWMRRKAAARQAAEAQPAVQTESIPDVPAAPPPAA
                     AEADGGPAIEAHVPVDAHVPAAEPPAQGHDADELPSLDSLQGIASDYSAFMRAGVDER
                     TRGAALKKLFSDPHFHFEQMDKLDIYIDDYGIPDPIPPAMLAGLEQARSLLFSEPATA
                     QESEPSTSEAAPVGGDDSSSMPAETPEEASKNEAVDDSVEDADEMSGEEPKQA"
     gene            complement(29355..29822)
                     /locus_tag="VKZ48_04315"
     CDS             complement(29355..29822)
                     /locus_tag="VKZ48_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011903241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3305 domain-containing protein"
                     /protein_id="HLU77115.1"
                     /translation="MHRTSFTVGVMMERRLLANRWQSVQWTPIAVDPDAGPADSPQLH
                     YSDAQIERWLHAGFELRLYPDEAEGYFLNCTTAEPAVFVAWRLEGDVAVPWAVTASYN
                     EAARLLDSGEEVQGVPMPKEILREVSAFVSLHYKPGPKKRSRPPSFKGAQRDE"
     gene            30060..31139
                     /gene="moaA"
                     /locus_tag="VKZ48_04320"
     CDS             30060..31139
                     /gene="moaA"
                     /locus_tag="VKZ48_04320"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HLU77116.1"
                     /translation="MNERIIPITDAGRIPTRPDEAHTAGKALLDTRSRPLRDLRISVT
                     DRCNFRCTYCMPKDVFGKDYQFLEHAALLTFEEITRLARVFKAHGVEKIRLTGGEPLI
                     RRNVERLIEMLAGIGGLELTLTTNGSLLARKAQALKDAGLDRVTVSLDSLDDAIFQSM
                     NDVKFPVAKVLEGIDAAARAGLTPLKVNVVVKRGVNDHSIVDMARHFKGSGHIVRFIE
                     FMDVGATNGWRMDDVVPSAEVIRRIDAEMPLEPVGPNYPGEVAQRWRYVDGSGEIGVI
                     SSVTQAFCNSCTRARLSTEGKLYTCLFANAGFDLRALVRDGASDEAIADAIAGVWGAR
                     ADRYSEIRTAETARGKKIEMSYIGG"
     gene            31157..31993
                     /locus_tag="VKZ48_04325"
     CDS             31157..31993
                     /locus_tag="VKZ48_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HLU77117.1"
                     /translation="MKPEITRTSRPLTFTVEATNELGEVLPTPIAGENPLTLYLDKRE
                     ILTLMTLGQAPEALAIGYLRNQRLVRSIDEIASVQVDWETDSVAITTRQGVADLDSKM
                     EKRTVTTGCGQGTVFGDLMEEIDTIRLRDDVQLREDTLWAALDAVRRRETVYKAAGAV
                     HGCALLTNEDGASEVIHFVEDVGRHNAVDAIAGQMWLDDIDGSDKIFYTTGRLTSEMV
                     IKAAQMRIPFLVSRSGLTQMGHQVATQVGMTMFGRAYNKRYLLFTGKHRFVKTSMPTM
                     LA"
     gene            31990..32580
                     /gene="mobA"
                     /locus_tag="VKZ48_04330"
     CDS             31990..32580
                     /gene="mobA"
                     /locus_tag="VKZ48_04330"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012353329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HLU77118.1"
                     /translation="MSAANDTTGLILAGGQGRRMGGVDKGLQPLRDRPMVEWVLERLQ
                     PQVADVIINANQNRDLYARYGHPVAGDEIGGFAGPLAGLHAGLKSTSRAFVLTVPCDS
                     PFLPHDLHARLYAASQAADADLAVAKTGDQAHPVFALVRTRVLPHLEAFLQGGGRKID
                     AWYATLKVVEVPFDDEADAFSNINTRDELRAHEQRA"
     gene            32592..33869
                     /gene="glp"
                     /locus_tag="VKZ48_04335"
     CDS             32592..33869
                     /gene="glp"
                     /locus_tag="VKZ48_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HLU77119.1"
                     /translation="MDRAMNDIKKIISCMDDYDPDALVVPKAHEAIRACLKPISGAER
                     VNVRASLGRVLAEDIISPINVPAHNNSAMDGWALRGADLAADGETRLREVGVAYAGRA
                     YAGTVKQGECVRIMTGAVMPEGADTVVIQEVVRSEGDSIIVPPGEKPGQNVRMAGEDL
                     SEGKPVLRAGQQVRPAELGLMASLGMAEVVVYRRLRVAFFSTGDELASVGSVLKEGEV
                     YDSNRYTLYGMLQRMGCDLLDMGVVRDDPDSLERAFREAAANADVIITSGGVSVGEAD
                     YTRDLLGRIGEVLFWKIAMKPGRPMAFGKIDNAYFFGLPGNPVAVMVTFYQFVRNALL
                     FLSGRTGETELPLLRVPCTTAMRKRPGRTEFQRGILSRDAQGQWQVAVSGAQGSGVLR
                     SMSEANCFIVLEHDRGNVQPGDLVSVQVMEGMV"
     gene            complement(34014..>36041)
                     /locus_tag="VKZ48_04340"
     CDS             complement(34014..>36041)
                     /locus_tag="VKZ48_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLU77120.1"
                     /translation="ITTLAARMLRQHLEADRCAYAQFDADEDAFVVVGDRTQREDGAV
                     GRYRLRDFGEQFGRALHAGQTFASADLRQSDPTSADNAFDRLGIRAIVCAPLVKQGRL
                     VAALALHQMQPRAWTRDEIELVRLVASRCWEAIERARVARNLRDSEARFRAVVETTPE
                     FVKIIRRDGTIEYINNAGLAMLGAESLDAVRGQPLVNFIVPRRRATWQTNHERVCAGE
                     QLVWQFEIVGLDGTRYWMETHAVPITMADEGLAHLGVTRDITDAKRIEREREQIINAE
                     RAARAEAERVVRMKDEFLATLSHELRTPLSAVLGWAQLLRRRVMNAADTAAALEAIER
                     NTKAQQQLIEDLLDMSRIVSGKVRLEIQPVRLPEVVDAALDVVRPMADAKHVRLALEV
                     ERDVPAVNADPNRLQQVLWNLLTNAIKFTPSGGEVTVRLGRADSALMLQVADNGVGMS
                     AEFLPQVFDRFRQRDSSTTRAHGGLGLGLSIVKSLVELHGGSIRAESPGEGQGATFTV
                     LLPIPAVAAMPPEIAADDGAGATEADVPSPDAPSLSGLKILVVDDEPDARELLRRMLA
                     EYDAEVSVAASADEAMACLAQERYALIISDIGMPNTDGYALMRRIRELRPELGGRTPA
                     IALTAFARADDRYRALSAGFQMHIAKPVDPVTLALACARMAEVAGRGVEQA"
BASE COUNT         6378 a        11939 c        11159 g         6565 t
ORIGIN      
        1 acagcagttc gcgcacggtg acgccgaagg ccttttcgac gatgccgccg tgcttgacgt
       61 tgccggtgag ctggatgggc agcgtgccgc gcgaacgacc catgccgtaa tcgcggtagt
      121 actcggcgcc cttgtccaga atgatcggca ccgaagcgag cgagatgacg ttgttgatca
      181 ccgtcggctt gccgaacaga ccttcgatcg cgggaatcgg cggcttgtag cgcacttcgc
      241 cgcgcttgcc ttccaggctt tccagcaacg aggtctcttc gccgcagata taagcccccg
      301 cgcccatgcg tacttccagt tcgaacttct tgcccgaacc gagaacgtcg tcgccgaggt
      361 agcccttggc tttcgccact tcgatcgcgc ggttgagcgc ggcatgcgcg tagggatatt
      421 cggaacgcag atagatgtag ccctgcgtcg cgccgcaggc gataccggcg atggtcatgc
      481 cttcgatgag cacgaaggga tcgccttcca tgatcatgcg gtcggagaag gtgccggagt
      541 caccctcgtc ggcgttgcag accacatact tctggtcggc ctgctgttgc agaacggtgt
      601 tccacttgat gccggtcgga aacgctgcgc cgccgcggcc gcgcagaccg gaatcggtca
      661 cggccttcac cacatcggcc ggactcatgg ccagcgcgtt cttcaggcca cggtagccgc
      721 cgtgctcgat gtagtcgccg atcgaggtcg gctcggtgat gccggcgcgc gcaaaggtga
      781 ggcgctcctg attcttcagg tagggatgct cttccggcgc gccgatacgc aacgcatgct
      841 cgttgccttc gagaaagccc gcctggaaca gccccggcac atcgtcgggc gtgaccggac
      901 cgtaggcgat gcgcttgtcg ccgacctgca cttccaccag aggttccagc cagagcatgc
      961 cgcgcgaact gttgcgcttc accagcacgt ctttcttctg cgcagtggct tcgatgatga
     1021 tgcggacggc gacctgatcg gcaccgaccg agatagccgc cgaatccatc ggcacataga
     1081 cgacgatcgg cgcgctcatg cctgcaactc cccgacgacc tcatcgaaac gctgcgcggt
     1141 gacgcggccg tgcagacggc cgtcgaccat catggccggc gaacaggcgc agtttcccag
     1201 gcaatagacc ggctccagtg tgtacttgcc gtccgcgctg gtgcccttga gcttcgtgcc
     1261 gagcttttct tcggcgtgcc gggtgagcgc ctcgacgccc atggactgac aggcttcggc
     1321 ctgacacacc tggatgacgt gcttgcccgc cggagtggtg cggaactcgt gatagaagct
     1381 gatcacgcca tgcacttctg cacgcgacag attcagcgac ttggcgatgg acgacacgta
     1441 gtcggacggt atgaagccga gttgatcctg gatggcgtgc aggatcggca gcaatgcgcc
     1501 cggtttttgg aggagatccg cgtacagctt ttcggcttcg gcgggcgacg ggatggtgtt
     1561 cattcaagac cctctgtgct tggctgcgcc accgttccca gctatgcttc tagggagcat
     1621 acggtacgca atccgtttcg cgatatcgaa gaatgctatg cgccatattt ttgttacgca
     1681 ataagagata cttagccaat attcttcgag ccaggaagca caacctttag gtttctataa
     1741 gcatatgttc aagatactca tcaagccgca atggctgatt cggggagacg aggcggagat
     1801 gcgccaagcc ttaccccgcc tgctcgaatt gctggcgctg ctgcatgaag acggcaacct
     1861 cgcgcgcgcc tgtcgcgagt tggatatgtc ctaccggtat gcgtggggga tgatccgcga
     1921 cggcggcgaa ctgttcggcg caccgctggt ggaaacggcc aagggtcagg gcaccaaact
     1981 cacggagctg ggccagcggc tgctatgggc ggaaaagcgc ataaacgcgc gccttgcccc
     2041 ggtgctcgac agcctcgcca gcgagttgga agtggaaatc cagcagacgc tttcgcggca
     2101 tgacactgcg ctgcgaatcc acgccagcca cggctttgcg gtggagacgc tgcgccgctt
     2161 cctttcggag cgcaatattc cgctggagct gaagtaccgc ggcagcggcg aagcgctcgc
     2221 ctcgctgtgc cgcgacacct gcgaagtggc ggcctttcat gtgccgacgg gcgacttgca
     2281 ggaaagggcg ctggggcagt tcatgtcctg gctcaaaccg aacagccaac gcctcatcca
     2341 tctggctacg cggcggcagg gcatcatggt cgcgcacggc aatccgctgc gcatcatcgc
     2401 cacgtccgac ttgctcaagc ccgatgtacg cttcgtcaac cgccaaccgg gttccggaac
     2461 gcgggtgctg ctggatttgc tgctcgagaa cgaacgactc gacggtcggg aaatccaagg
     2521 ctacgacacc agcgaataca cacatgcggc ggtagccgcc tacatcgcca gcggcatggc
     2581 ggacgcggga ttcggcgtgg aaacggcggc gcgcaaattc aatctggatt tcgtgccggt
     2641 tgtgaccgaa cgttatttcc tcatctgcaa caccgcctcc ctggaattgc cggcggtgaa
     2701 ggagatgctc ggcattctgc gcaacaacga atttcgcgcc gcggtgaatg aattacgcgg
     2761 ctatgacgcc accgagtgcg gcaagatgat gacgctcgaa gaagcgtttc ccgaattcgc
     2821 cgcaatggaa aagccggaac ggcgccccgt caagaagaag ggtgccgcgg cggcctgagc
     2881 ttgcaaccgc cgcagccaaa gccgcataac cgtgctgtct ccgcacggac ctcaatacgc
     2941 gcacgcctcc acccgcccgc tcagacttgg cgcttcgccg cctttgaagc gcggaaggct
     3001 taccgctcgg gcgctgaagc gacgccggct ccggcgccgc gtcgccgttc gagccgccgc
     3061 ttagccggcg cagcagtgtt tgtacttctt cccgctcccg caaggacagg gatcgttacg
     3121 accgaccttt tcgccctcgc gccggaaggg ttcacgcggt ttgccgcccg tcagccgatg
     3181 gtcggcgtaa tagcggttga tgagcgtgac attccacgcc atgtcgtcga gcagcgatgc
     3241 gcgccggcct tccgagaggg gcggtgtgcg cagatcggga tccgggtggt gctcgttcgc
     3301 cagcatgagc atcggggtca gcagactttc gtcgtcgtcc tcgtacaact cttcccataa
     3361 ggcataacgc tgctcggtgc ccttgagaaa accgacggcc cagtcgttgc ccagcgcact
     3421 gccctgctcg tcttcggcca tgatcgggac atacacctca tccttttcca ggaggctgaa
     3481 cagttgcgcc cctatcgtct gccaatggcg ctggagcaat tcggcaatgc ggcgcgactt
     3541 cgtcgcatcg gtgaaaggcg gtgtctcatc gccaggctgc aggccgtaca cgacggccag
     3601 atactcgctt gcaggcggat gctcgggccc ggcgagcagc gcggcgaaat agccgtcgag
     3661 cgtttccaga ttcattctgt ccgcggaagg caattcctcc agcagtgcag agagttcgtc
     3721 gagttcggct tcattgaggg ggttttccac aaacttcatg gtcgtctccc ggacttgtcg
     3781 ttgttgtcgt gctcgccctg acggcgacgc ggctcaatgc agatgctgcg ggttaccgca
     3841 gcactgcttg tatttctttc cgctcccgca cgggcaggga tcgttccgcc cgaccctgcc
     3901 ccgctcgcgt gcagcgtttg cattcgcgcg caaaggttcg aaatagcgat ggatcaacgt
     3961 gaggtaggcg gtcgcacgac ggatgaggtc gccgcgctct gcgtcggaaa tcggtgcggc
     4021 gcgcaactgg gcatccgcat tgtgttcgtg cgcgagcgtg aagatcggga ccatgaggtg
     4081 cacctcgcgc gggtcattca gcagcgccga ccacgatttc ccgcgcaggc gcagaccgcg
     4141 cagaaaacca ttggcccagt cgttgccgag cgcacgcccg ttcgcatcgg tatcgaaatt
     4201 gggcagatag acgctgttct cccgcaggcc ggcgtccaat gcggcgcgaa tgccttccat
     4261 caactgcgcg agcagcgcga ccaggctttc gacgccgtcg aaacgctcca gcgcctgcag
     4321 atcctcggca tcggcaatgc cgcacaccac cggcagatat tcgctctccg gcacgacgtc
     4381 cggcccggct gccagcgccg caaaaaagcc gtccagcgtc gggacattca tcgcgcgccc
     4441 tgaaggcagc gaggcaagca gttcgcccag ttgccgcagt gcggtctggc cgatcgatgg
     4501 aaaggtcgaa gcctgcatga aggatgagtt cgttacggaa gacaggggct agttttgcac
     4561 aatgcaagcc ccgtcacact gcgcgaatgg cttgacgtca gcgcgcatgc gcgttagccg
     4621 catgccggcc gacgcggcga ctcagaccgt tgtcgcgacg ctgtcgatca tctgctgcag
     4681 cggcaccggc cgcgccaaca gataaccttg cgcatactcg atgccgagcc gacgcagata
     4741 agccagttcc gccggcgtct ccacccgctc ggcgacggtg gtcatggaga acttgcgcgc
     4801 gatggtctgg atggcgtcga ccagcgggcc gttgacgtcg tcgcggtcga tgccgcagat
     4861 gaagctcccg tcgatcttga gcacatcgat gggcaggtcg cgcaattgcg acagggaggt
     4921 catgcccacg ccaaagtcgt cgagcgcaaa gcgcaggccg cgtcgcctta aggtctgcat
     4981 gagttcgcgc gctgcggcga gattttcgat ggcggcgctt tcggtgattt cgaaacagat
     5041 gcgctcgaag gggattccat gacgttccag gtgttcgaga atataggcgc cgatttcccc
     5101 atccttcagc gtaatcgccg agagattgat cgacgccgta tcgagccgat gccagccatc
     5161 cggcccgaag ttctcggcca gcgcgcgcgc cgcatgttcg atcacccagc ggtcgatctg
     5221 gcgcatgagg ccgtagcgtt cggcggcagg catgaacacc gcaggcggca gcagtgcgcc
     5281 gttttcgtcc agcatgcgca ccaggatttc gaaataaagt ccgtcgccgt catgcgcgac
     5341 gggatggatg gcttgagcat acagttgcag acgatcggcc tcgatcgccg cacggatggt
     5401 cgagacggca gccatttcga ggcgcatggc gttcagtgcc tcgtccgagc cggagtggtt
     5461 gcagatgcgg gtgccgccgc tgcgcttggc catgtacgcc gccatgtcgg ccgctctcac
     5521 cacttcggac gggtcgcaga agtcggcgtt gatttccact acgccgatac ttgcgcccac
     5581 cgcaaacaga cgcccgtccc actcgaagcg atgcccggcc accgccttga gcagcgtctc
     5641 ggccagccgc accgcctgct cgctcgtgca gccgtacatc accaccgcaa attcgtcgcc
     5701 gcccagccgg gcgaccaggt cggagcgacg cgaacgccgg acgaacagat cggccagctc
     5761 cttcaacagc gcatcgcctg ccgcatgacc gcaggtgtcg ttgaccagct tgaactgatc
     5821 caggtcgagc acgagcagcg cgtgggattc atgcggccgt acggatgcgc acagtcgcct
     5881 cagcgcctgc tcataggcat ggcggttgac caggccggtc atcgcatcgt gcgtcgcacg
     5941 ccattcgagc tgctgcgccg cggctcgttc tgcggcgata ttacggaaca cgatcaccgc
     6001 gccgtcgatc tctccttccg cgttgcgaat gggcgcggca gaattgtgga tgggaatgta
     6061 ctcgccgtta cgcagacgca ggagtgcatt cggaatgcac gcgctgctgc tttgctgcat
     6121 ggcaagctgc gcgatgtcgc cgagatccac gccgtgcttc tcgtccttca ccacgaacac
     6181 ttcgacaaac gggcggccga ccacttcttc gtcgcgccag ccgatgatcg attcggccgc
     6241 gccgttcatg gccgcgatca cgccatggcg atcggttgcg atgacgccct cgccgatgga
     6301 ttcgacaatc acctcggcgc gctccttctc gcgctgcaag gcgccctccg ccgtgcgcca
     6361 ggcggcggcc acctgcgcga actcgttcgc cagatcgctc agctctgtcg tcgcacgcat
     6421 cgcatgcact tccggcgcgc cggattcgcc ggtacgcatg cgccgcattt cgctgcgcag
     6481 atggtcgagc ggctgcaaca tgcgcgagac caacagccac atcaacggaa acagcagcat
     6541 cgcaagcacc gcccctgcga gcagcatgcg gttggacaac tcggcgagcg aggcatatgc
     6601 cgcttcgttc gtccctacgc cgaccagcgt ccatccggcc gatgagatct cgcggaaggc
     6661 atacagcccc gacacgccgc gatgatcttc gtcgatgacc gaagcaacct ctttcgccat
     6721 gccccgctcg atcgtctgcg caacttttcc gagcgcatcg agtggctgac cgacgagccc
     6781 gatatccgca tgcagaccga tgtagcggtc gtcggtgacc acgataaaag ccgactcgcc
     6841 gcgcatgtcc gtgctcggct tggcgatcca caactcgtcc tgcgtcagcg caaaagcacc
     6901 gacgactacc gcctgcaacc ggccggactc atcgtgcacc ggataagtga gcagcgtaac
     6961 cggcttgcgg ctcagtggac tgaggaaagg ccggtgaatg cgcggcacgc cgttattggc
     7021 gatggtgtcg cggaaccacg cttcctcatg caccgaacgc acttccgtcg ccgggcgcga
     7081 ggcgagcagc cgcccctccg catcgaacac gctcaccgct tcgaagcgtt cctgcaggcc
     7141 gaccgccatg ttgaggaagc gatggagcgt aatcggattg tgaaagtggc tgcgatcgat
     7201 gttcgccgcg aactcgctca acgccgcgcg tgccagacga agctgccgat cgatatcgac
     7261 ggccacgcgc tgcgtgtagc ttatgtgctg ggcgacaccg gacgcgagca cggtgtgctc
     7321 cgacagatcg gaatgcaggt tggtgacggt aatgatgccg atcgtggcca agcccgctgc
     7381 cgccagcgcc gtcctgccct tgagcgtctt gagcaaaact ccccccgtga atcagaacga
     7441 cgaccgcgcg gggccgttca caattcctcc ctatgtcata ggatcgactc atcgcgaggg
     7501 atatgtatgg catacgtacc gtgaatgcga attacttcac gtctgcggtg ccgtgcagtt
     7561 ctcaatcgct ctcaagttgg cgaggatgga ccttcgccaa tcgctgcgca tcgcgacgac
     7621 gatgccgagc ccgatatgac acgacggaaa tgcctggtcg cggcgctgtt gctgtggctc
     7681 ggcatggcag cgccgtgcct tgccgcttat cccagccagc cgatcaccct catcgtgccg
     7741 ttcccgcccg gcggcaccag cgacgccgtc gcccacgcgt tgcgcacccg cctcgcggaa
     7801 aacctgaaac agcaggtgac gatcgactat gtcgcgggcg gcgccggtgc ggtgggcgcc
     7861 atgcgcctga aacacgcggc gcccgacggg cacaccatca tggtggcgtc gctcaccacc
     7921 tacgtggccg gtccactgtc caacaccgcg gtgaactacg atagcgcacg cgacttcgac
     7981 ctgctcaccg tcgcagtgca tgtcccgaac gtcctcgtcg tgcatcccga tgtgcctgcg
     8041 cagacggtcg cggaattcgt ggactatctc aaacgccatc ccgaccaggt ccgcttctcc
     8101 accagcggcg ccggcggcca ggatcaactt tccatgcagt tgttctggca gcggaccggc
     8161 acgcgcggcg tattcgtcca tcgcaagggc tccgcacccg cagtggcgga cgtggcggca
     8221 ggacgcgtgc aggcatcgat ccagaacctc agcgtcgccc tgccctatat cgaaaacgcg
     8281 caattgcggg cgctggccgt gaccggatcg gaccgctcct ccgcgatcgt gtcgacgccg
     8341 accctgaccg aactcggcgt gcccggcatc gagacctatt cctggcaggg gatcgccgcg
     8401 ccgaaaggcc tgccgccggc cgtcaagacc cgactgcaca aagcgctcgt ggccagtctg
     8461 gatgcgcccg aggtccggcg ccatctgacc cgagccggct tcgaggtcgt cgccaacacc
     8521 cctgcggaat tcgagcgttt cctgcatgcc cagcgacacc actggcaggc catcatcgat
     8581 cgcgccaggc gcacatcgcc ctgaatccgc tcgcgcgggc acgcgcgacg ccggatagaa
     8641 tcaagcgact catcgaccgg gcattcgtgt gtacgtttca cagcttctcg gtgtgctgct
     8701 gctcaccgcc atctatcttg ccggcgccca actggcggcg gcgctcgatt cggccattcc
     8761 cggctcgatc tacggactgc tgggctgcgc ggccgcaatg ctcgccatgc cgagagccac
     8821 gcagtgtctg cgccccggcg cggcactgat gctcgtgctg gtgccgctgt ttctggtgcc
     8881 gatcacggtg cgcatggccg tgcgcatcga cttcggcaat ccggccacct ggcgcgccat
     8941 cgccgtgctg ggctttgcct gcctcatcgg cgtggcggcc accggcatct tcgcgcgtct
     9001 gctgctgcgc aaggaggcgg gccggtgagc gtactggccg cccttgcact cagtgtgctg
     9061 ggctatgtgg cgggcgtgtg gctccagcgc cgcaccggct ccaagccgta catgcagccg
     9121 atcctgctgg ggatgttcgt ggtggtggcc gtgctggccc ttctggacgc gcccgccacg
     9181 gaagactatc tcgcgcacaa cggcctgctg ctcgatctgc tgatggtcgc agtggtcgct
     9241 tttgccatac cgctggtgga caacgtgcgc gtggtgctgc gcgatctgat cggccttgcc
     9301 ttcgtcgtgc tggcggccgg cgccttcatc gcctcctcga cactggcgct ttgttatgtc
     9361 gcaggcatcg acgcggcgtc cattgccgcc ttcagcctgc gttcggtcac caacccgatc
     9421 gccatcgcgg tggccgaaca gaacgccatc tcggtcgatc tggcgatgct gggtgtgttc
     9481 atcaccggct tcgtgagcgt cgtggtggtg gagcccatcc tgcatcgcat gggcatcacc
     9541 gacccgcgcc atttggggct ggtactgggc attaccgcgc acgcgctggg catcgcccgc
     9601 gcgctggaaa tcagctctct tgccgcggcc tatgcgaccg tcggcatgat ctgcaccgga
     9661 ttgctctacg ccttcgccat accctcgctg gtgcgcctgc tgccgctgta gcagcggcta
     9721 tttgcgcgcc gcgccttcgc gctcctgctg acgattgatg gtgctgacga ggtcgttcaa
     9781 ctcctcggca atgatcgcca gcacgtcgtc aactgtgacg atgccttcca gcacaccgtt
     9841 cggcgacagc acggggatgc ggcgtacgcc gtgctgtcgc atgcggtgca gcgcgtcggc
     9901 aacggagtgc gttgtggtca cggaaacggg attcgcctcc atcacctcgc ccaccttgac
     9961 cgtgcgcgcg tcgagatcgc gcgcgaccac ggacaacacg atgtcgcggt cggtcaggat
    10021 gcccgtcacc ttgcggccct cgacgccgtc ctccaccacc accagcgatc ccacgttctc
    10081 ttcgcacatc aggcgcgcgg catccgcgac gctcatttcc ttacgcgcga ccaccaccac
    10141 ggggttacag atctggccga tttgcatgct ctcctcctcc aaggtgttgc ttcagggcgg
    10201 tgctgcggac gccgcccggc aacggaagag cttaacggcg cgcgcagcga tgagcaaagc
    10261 gccgtatcaa atcgcttgtc tagaacttca gccagccgcg cttgaggaat gcggccgcga
    10321 tcaggccgaa caggaaggcc acgccgacat tccagatcgc cagcgcggtc gtggtgacgg
    10381 tgacgaaacg ctcacccttg tcgcggctga aatcgcagga gccgagcgcc aactgcgcac
    10441 cggtgagaaa gaggatcgcg ccgagcaccg gcacggggaa gagccgcagc agcacgtcga
    10501 tcgaggcgct gaagaacagc gcgagcacca gtagcaacac gccgaacatg acgggcgcgc
    10561 cgcccgtgcg cgcgccgaag cggatgtgtc cggcaagacc gccggcgccg tggcacatgg
    10621 gcacgccgcc gaacagaccg ccgaaaatgt tcatcagccc cgtggagcgc gaaacccgct
    10681 gctccgtcac cggacggtcg gggaacagac ggttgttctc ttccgtgatc gcgatcacgg
    10741 cattacccag cgtgagcggc aattgcggga gcaccagaaa cagcagcgcc accgaccagt
    10801 cgctccaggt cgtgtcccgc agcgcgaatt ccggccagac gaaaccgggc cgcagttcgc
    10861 gcagttcctc caacatcgcg gggtcctgaa agagcgcgac cgccgcaccg aacagcagca
    10921 gcaggaacat caccggcaca tagcggttct cgagcagcag caacgcaccg aacagggcga
    10981 tcgcgccgac cagccagccg tcggtgatca tgcgcgcgcc ttccagcatg aaaccgatgc
    11041 ccagccccag cacgatgcca agcacgaccg gccggctgac gagcgcggtg atgcgcttgg
    11101 ccaagccggt caccgcgagc agcagccaga cgaagccggt cagcatcgat gcgcaataga
    11161 ccacctgcgc ggtgatcgcc agctggccag cctgcgtggc gacgaccgcc ccagccgctt
    11221 tcatgggctg cacggggaaa ggcgtgcggt agtacaggcc cacggcgatc agcgccacgc
    11281 cgaaggacaa caggatcccc atcgccggca cgccggccac aacgatgtac gccgccagaa
    11341 agggaatcag cgtgccgatg tcgccgaacg caccggcgaa ctccgtacgg gagaagcgca
    11401 ggcgtcgtcc gtgaaaggac tcgctgcgcg gcatgggaat atcaggtctg gacatgaaga
    11461 agagcgatgc gggcaccaga ggccggggcc cgcatcataa aggctcaggg cgcgggcggc
    11521 gcgaaaccgt gctgcttgag cagcgcctgc cctttgtcgg acaggatata gtcggcgaac
    11581 ttgtgcgcct cggcacgatc gctcgcgacc gtcagaccgt aatccgcacc cacggccagc
    11641 gcctcgggca cttgcacgat cttgagcgac ggcacctcct tttgcgccag cagcgcgttg
    11701 gtgcagtagg tcaggaagat atccgcctcg ccttcgtcca ccagcacgcc gtacaccaca
    11761 cggtccttgg gcggcggcgg cgaatccgga ccgccggtga gacgcagcgc cttgtccgac
    11821 aacgccttcg cggcgccggg ttcaagcgct tcggccttgt cgtacagcgc catggcgtaa
    11881 tcgccggaag gatcggcctt gggcgtggac atgccgagct tcacctgcgg atcgagcatg
    11941 cgctcgagca gcgtctcggt cgtgacttca accttcggcg atgtcagcgc gcacaggcgg
    12001 ttgcgcgcga acatcgtcac cggcccgctc ttgccggcct tgtgcagagc ttgcgggtgc
    12061 gccatgttcg ccgaggcgaa cacgtcggcc ggctcgccct cggccagccg cttgcgcagt
    12121 agaccggagg ggccgaactc gcccttcacc gcgacgccgt gcgccttggt gtacgccgcg
    12181 ccgatgtccg tcatcaccgc cttgaggctg cctgcggcat aaagccgcac ggcttcctcg
    12241 gcattgaccg cgctcgtgaa cgccatggcg gcgaccagtg caacgatcaa acgcggtttc
    12301 atacacccga ctcgttggcg ttcgggaaga acagctgttc gccgccgatc ttgtacccgg
    12361 cgatggcttt ctggccgtca tccgagatga tccagtcgat gaacttctgg ccggcgtcct
    12421 tcttcacgtg cgagtgcttc tccggattca ccaggatcac gccgtactgg ttgaacagac
    12481 gcttgtcgcc ctcgaccaga atctccaggt cgccgcggtt cttgaaggaa atccaggtgc
    12541 cgcggtcggc cagaatgtag ccgttcatgg aggcggccgc gttcagcgcc gggcccatgc
    12601 cctgccccgt ctcgcgatac caggcgccct tgtccttggc gatatcgatg ccggcaacgt
    12661 tccacagctt cacttcagcc atgtgcgtgc cgctcttgtc gccgcgcgaa acgaaaggcg
    12721 tgttcgcgta cttgatcttc tgcagcgcag cggtgatgtc cttcatgccc ttgaccttgg
    12781 ccgaatcgct tttcggtccg atcagcacga agtcgttgta catcaccgga tggcgcttca
    12841 cgccgtgccc ttcggagatg aacttctctt cggccgcccg ggcatgcacg aacaccacgt
    12901 cggcgtcgcc gcgacgcgcc gtatcgagcg cctgtcccgt gccctgcgcg ataacgcgca
    12961 cctggatgcc cgtctccttc tcgaagatgg gcaggatgtg cttgaacaga ccggagttct
    13021 cggtcgaggt cgtcgaagcg acagtgatga atttatcctg cgcctgcgca ggcagtgaca
    13081 gcattgccgc agccgcaatc gcgactgcga acaactttct gcgcttcagg tccatggcaa
    13141 ttctcccttt aagaaagcgg cggcttccgc cgtcgccggt tgcttgaaaa actggtcgac
    13201 cggcgcgcgc tccaccagcc ggccgccgta aatgaacagc acctcgtcgc caagccggcg
    13261 cgcttgaccc aggttgtgcg tggacatgac gatcttcgtt cccgcggcgt gaatggtctg
    13321 cacgatgcgc tcgacttccg cgctcgccgt cggatcgagg ctggccgtag gctcgtcgag
    13381 gaacaggact tcgggacgca gcgcccaggc acgcgcaagc gcaacacgct gctgttcgcc
    13441 gccggacatg acgcgtgccg atcgttcggc aagatcgcgc aggcccacga cctccagcgc
    13501 ctcgagggcg cgctcgcgtc gctctgggcc ggagacaccc tgcagtttca aggcgtattc
    13561 gatgttggca agcgcggagc ggcgcagcat taccggtcgc tgcagcacca tggcctgacg
    13621 accgcgcgca cccgtctcca cgttccaacg cacaatgccg ccggtcggtt tcaacaggcc
    13681 gtgacacaag cgcatgaaga cgctcttccc tgcgccgttc ggaccgacga tgatggtccg
    13741 cgggccggca tcgaacacga ccgaaatgcg gtcgatgatg gtgtggccgt tgactgcgaa
    13801 gctcacctcc tcgagggcga gcggcaggat ggacggcgac agggaagcgg gcggctgctt
    13861 atccatagcg tcgctgcgcc gcctccttgg tgagatacac cgccgcgttg agcgtaatga
    13921 ccagggtcat cagaatgacg cccagaccca atgccaacgg caaatcgccc ttgctcgtct
    13981 ccagcgcgat ggcggtggtc atgacgcggg tgacgccgtc gatattgccg ccgacgatca
    14041 tcaccgcacc cacctctgct gccgctcggc cgaagccggc aagcacggcg gtcagcagcg
    14101 aaaagcgcgt gtcccaaagc aaagtgaacg aggccgacag gttgcttgcg ccgagcgagc
    14161 gcaactggtc ccgatattcg atccatgcgt cctcgatggc ctggcgcgcc agcgccgcca
    14221 cgatcggcgt gatcaaaaag gcttgcgcaa taatcattgc ggccggcgtg aacagcaggc
    14281 cgaactcccc caatggaccc gcgcgcgaaa gcagcaggta gatcagcagg ccggccacga
    14341 ccggcggcag gcccatcagc gcattgaggc cgacgatgac ggcattgcgc ccggggaaac
    14401 gggcgacggc gatcgtcgca cccagcggta agccgatcag cgtcgcaatc agtacggcgg
    14461 tcaggctgac ccgcagactg agcagcacga tttccagcaa agtctgatca aactgggcga
    14521 tgagcgctac cgcttggccg aaggcctgcg agatttcgtt caactaaatg ctcccgcgta
    14581 gaggggtggc cggcaggccg gcagaaaggc tgggagctta ggtaaatgtg ctgcccggga
    14641 caatatgatt agttcgacat atgtcctaag cgtttcaagc acatatgtct tgcttttcac
    14701 cagggcaata tctgatgcag gccgatgaac ctgcccgacg gcaccggcct tacggctggc
    14761 ggaaaccgct ctgaactgct tggaatccac gtgtgcagcg gcaaccgtcg cctacgcgcg
    14821 gcgtacaggt tgggctgcga cggactcgaa cgaccgaaaa ggcagcgagg ccgacgaggg
    14881 atggaccggt cagtgcagct gcaccgcgcc ggcgttgtaa tcctccagac ggatttccgc
    14941 agcctcgtgg tgctcgtaga ccagcgcggt ggacaccacg ccgtagacgt cgccgatggc
    15001 cgccattgtt tcggaaatcc ccgaagagga ttccgcctca agcgtcacga cgatcttgcc
    15061 cgactgcgtg gcggtcagca cttcggcccc gtccagcgac tggatgtttt cctgcacgcg
    15121 ctcgatgagc tccggccgcg cgtggacgag tatgcctgcg atgtgtacgt cgtcttcaaa
    15181 catcgttgcg gtcctgtgca gaatcgttga atggaagatc ggttccattc attcgactgc
    15241 aaggcccgtg ccgctaatgc cgggcttcaa gccgcgcgta tggggatctc ccgccgccat
    15301 tggcgcggcc cgctgtcgtg cacggaaacg ccttccgcgt cgacagcgac ggtcacaggc
    15361 atgtcccgca cctcgaattc gtagatcgct tccatgccga gatcttcgaa ggcgaggaca
    15421 cgcgcagtgc gtatcgcttt cgagaccaga taagccgccc cgcccacggc gatgcaataa
    15481 acagccttgt gacgtttgat ggcttcgatg gcggcggggc cacgttccgc cttgccgacc
    15541 atgccgatca gtccggtctg cgcgagcatg gcttcggtgt atttgtccat gcgcgtggag
    15601 gtggtcgggc cggccggacc aacggcttcg tcacgcaccg gatcgaccgg gccgacgtaa
    15661 tagatgaagc ggttgcggaa atcgaccggc agtttttccc cgcgcgctag catttccgtc
    15721 aaacggcgat gcgccgcgtc gcggccggtc agcagcttgc cggacaacag cagccgatcg
    15781 cccgatttcc aacttgcgat ttcctcacgg gtgatggcgt ccagattcac gcgtcgcgcc
    15841 ccttcgggcg ccgcgtaatc gagcttgggc cacagatcca gactcggcgg ctgcaactgc
    15901 gccggcccgc tgccgtcgag cacgaagtgc gcgtggcgcg tcgccgcgca gttcggaatg
    15961 atcgcaaccg gtttggacgc cgcatgcgtc ggatactctc gaaccttcac atcgagcact
    16021 gtggtcaggc cgcccaggcc ctgtgcaccg atgcccagcg cgttgatccg gtcgtgcagt
    16081 tcgatgcgca gttcctcggt cttgttgcgc ggcccgcgcg ccttgagttc ctgcatatcg
    16141 atcgggtcca tcagcgcttc cttggccagc agcatcgcct tctccgccgt gccgccgata
    16201 ccgatgccca gcatgccggg cggacaccag cccgcgccca tttccggcac ctgctggagg
    16261 atgaactcga ccagatcgtc ggccggtacg agcatcttga acttggcctt gttttccgag
    16321 ccaccgcctt tcgcggcgac cgtcacctcc actttgtcgc ccggaacgac gctcatatgg
    16381 atgaccgccg gtgtgttgtc gcgcgtgttc ttgcgtccga aggcgggctc ggcgagcacc
    16441 gatgcgcgca gcacgttatc gggggcgaga taggcgcgtc gcaccccttc attcaccatg
    16501 tcctccaccg acatgttggc gtcccagcgt acgtccatgc cgatcttgag aaagaccatg
    16561 gcgatgcccg tgtcctgaca gatgggccga tgtccttcgg cgcacatgcg cgagttcacc
    16621 aggatctgcg tcatggcgtc cttcgccgcc ggcgattcct cccgctcgta ggctgcgctc
    16681 atggcgcgga tgaaatcctc cgggtggtaa taggaaatga actgaaacgc atcggcgatg
    16741 ctttgaatga agtcgtcctg cttgatagtc gccatgcggg cactcctgta aacaatgacc
    16801 ggaaagtagg atagcccgca gcgaaaggca agcgcctcac gccggcaaac tgaagtagaa
    16861 ggtcgcgcct tcgtccggcc ggccctctgc ccagcagcgt ccgccgtgac gcgagacgat
    16921 gcgctgcacc gtcgccagac cgatccccgt gccggggaaa tcgccggtat gcaggcgttc
    16981 gaataccttg aagagcttgt cggcatagcg catgtcgaaa cccacgccgt tgtcgctgac
    17041 ggagaagatc cagtggtcgg cttcgcgccg cgcctccacc gtgatgacag cgacatcgcg
    17101 tgtgctggag tacttgagcg cattggagag caggttggcc cagacctgcg tcatgagatg
    17161 ttcgtcgcac tccacggtcg gcagttcgcc gatgcgccat tcgacagcgg gggcctgttt
    17221 ttcctcctcg agactctggc aaacacgcgc cacgaggtgg cccatttcga ccggcgcttt
    17281 atgcagcgcg ctcttgccca tgcgcgagaa gcgcagcagg tcgtcgatga gctgcgccat
    17341 gtgcagcgcc gcggacgtaa tccgctccag ccgttgccgc ttggcggcgt ccaattcgcc
    17401 gcggaattcc tccagcagca tctgcacatg cccgtcgata tgccgcagcg gcgcacgcag
    17461 atcgtgcgac accgaatagg tgaaggattc catttcctca ttggcggttt tgagttcggc
    17521 ggtccgttcg gccacgcgcc gctccagttc ggcattgaag gtgcgcatgc gcgcttcggc
    17581 atgcttgcgc tcggtgatgt ccatcaccgc ggcaacggag ccgagcactt cgccgcgcac
    17641 gatgagcggg ttggtgccaa acgcgacgca gacgacgcgc ccgtcctgcc ggatcagttc
    17701 gacctccctg ttgatgatgg actcccgctc cgtgacgatg cgcttcagtt ctggcgtcag
    17761 tcgttcggcg tccgccgggc tggtgaggag agaccagtgc tggccgcgca actcgtcctc
    17821 gccataaccc agcagcttgc agaacacttt gttggcggag gtgatgcgac cacgcggatc
    17881 gaaggtcacc agcccgacga ccgcgctttc cattaccgag cggcggaact gatcggcacg
    17941 ctcgtattcc tccagcgtgc gttggagttc ggcagtgcgc tccgacacgc ggcgttcgag
    18001 ttccgaactc atgcgccgcg tctcctcctc tgcctgcttg cgcaccgtca cgtcgcgcac
    18061 gacgatggtg acgaaatcgc cggtctccgt ctgccagcgc gccgtggtga tttccaccgg
    18121 cacccggctg ccgtccttgc gcacggcgat cgcctcgtgc acgttctgat cgtcgcctcc
    18181 cggcggccgc tgcatccatt gcgcatagcg ctcggcgtac gtcgcctgca gttcgggcga
    18241 cgcgagcatc atcaaggatt gcccgatcgc ctcttcggcg cggtagccgt agagacgctc
    18301 ggccgccgga ttgaagtaga tgatgttgcc ctgctgatcg gccgagacga tcgcatcgta
    18361 ggcgttgtcg gcggcggcgg tgaaatgcgc ctgcgaggcg cgcatgcgtt cggcggcgcg
    18421 gtccgccgcg tcaatcgcac tcatccgcga ggcggcgagc gcgcgcatca tgatgaacag
    18481 gatcaggctg atcggccccc cgatcaacca gacggccacc tcgtcgccac ggtcgagcgc
    18541 cctgtcgaag gcgtcggtcg cggcaaagtg cagcgtccat tccgcgccgc ccacttcgat
    18601 cgtccgcaag tgctcgaagc gcggcggctc atcgggccgc aacgactcga agtacacgcc
    18661 actgtcgtac agcagcgttt cgggcaggcg gcggatgccg tcgtacacgc gcaaggtgag
    18721 gagcttcgca ctgcccggac cgaacaggcc atccagaaac tgcgacatga ggtaggaact
    18781 gaatacgaag gccgtgaggc gcgcttcgcg ctgatccacc gtctccggca cgccaccgcc
    18841 ggcatagacc ggcagataca tggtgaatcc ccgctgaccg acgcccccgt taagcagggg
    18901 gatggtcgag gtgacggcca ggcggcctgc acgcgctgcc cgccgcatgg cggcgcggcg
    18961 catcgggtcg gaccacatgt cgaagccgac cgccatgctg ttgggcatcg acgcggaatc
    19021 gtagtaggtc accgggaagt agaacgggcg ctcgccgggc ggctggatgt cgtaatcttc
    19081 acgccccagc gcgcgcatgg cctccagata gagcgccttg tcggcctcgg caacacgcag
    19141 agcaaggcca accgcatcga gtccctcgta accctgctcg acgttgatgt cctgcagata
    19201 gcgctgccag tccgcctggg tgacgaacgc actgccggcc agcaggccac gcgcaccacg
    19261 cagtacgctt gcatgcagat tgatgcgctc gtcgatgcct tgcaccgcct gctgcacttc
    19321 gtagtcgaag cgcagcttcg cgctttcccg attctgtgtg gcgaccagtt gccagacgac
    19381 gagggtaacg aaaagcgaca gcagcaatac cagccagggc ggcgccagcc gtccagccaa
    19441 cagggcgaag cgttgcgcct tcgcctcgat tgcccggtgc ccagtagagc cgtgcacttt
    19501 ccctccgcgc gatcacccac gccgccggct tcgatgccgg cgcatgtgcg tttatccttc
    19561 ttccggcttc ctgcggtcgt ctatacaggc tctggtcaag atcgttcgat cgatactcgt
    19621 ggcgagcatc cgcgcattcg cgatcactcc cgtcttcgat cgaatcttcc ctgcttctcc
    19681 tcctcgatcg atccaaaaaa caagggcgcc gcagcgccct gtgcccgaag atcggttcgc
    19741 gctgcctcaa gctttcaacg cttttcgatt acaccacatg caagccgcgc cccggcgtcg
    19801 cccgtcggct gagtgcgata atcgtccggt tttgcatgca cgatgacgct gcggcccacg
    19861 acatttgtct cgccttcgtt cacgctcatg ccgctcagat cgaagctgaa ggtcgcctga
    19921 ccggcgccgt ccgcacgcag gttgggcatg tcgccggcgt ggcggccgcc cggcacggca
    19981 tggccgtgcg ccttgttcag tggattgaag tggccacccg cgctggtggc atcgggcgcg
    20041 ctgcagtcgc ccttctcatg cacatggaaa ccgtgctgac tgttgggaac gagtccggtg
    20101 atcttgccgc tgacccggac cttgtcgcga tactgcgcga agttgaccgt gcccttcgcc
    20161 tggttgccgg cggtcggcac cagttcggca cgtgccgtgg gcacgtcccg ttgggcggtg
    20221 gcgcacgccg tcagcgcgag cgccgccacc gcaatcgtcc atcctgtcct catgccgctc
    20281 cttccatcga tagagtggag gaagcaggcc ggacgctatt gtgcgggttg cccctgcgcg
    20341 gaagacgtcg atgcgttcgg cgtcgccccg ccgccagcct gctggttgcg cctgtagttc
    20401 tctaccgcgc gatcctgcgc cttggctagc aattcggcgg cctgcttcgc cgcttcgtcg
    20461 gccttcgcct tcttggcggc agccgcggcg gcctcctccg gcgtaggcgg cggcagcttg
    20521 gccaacaccg gcatggcgaa cgacagcatc atgaacagga tgacagctcg catcgtcgtc
    20581 tccttcgcgt caggtctgtg tgccaggcgc cgggccgcgt gccgagggcg gcacttccgg
    20641 cgtaacggtg ggcggaaccc ctgcagaagg aatgcggccc gccttcacgt cctcatacca
    20701 gtaatagtga tgctccttgg cccaggtctc gtccacctcg ccggtggcca tcgcattgaa
    20761 cgcgcctttc atgccgatgg tgcccatgta gatgtggccg atggcgccga ccatgaacag
    20821 cgtggcgccg atgaggtgga ccatgttggc cgtctgcatg gtctgacgcg tctgatcgaa
    20881 gttggggaaa tcaagcacca gtccgctaat gccgaccacc agaccgagca gcgacaaccc
    20941 gccccagaac caggccttct cgccggcgtt gaagcgatgc gacttgggct cgttatggcc
    21001 tctggaaaac aaaccgccaa agcgtctgag ccactctcta tcgtagtcgc gccagatgtt
    21061 gtccttcacg aaggtgaaga acatcgccag cgtgcagacg atgaacagcg gcccgacgaa
    21121 gttatgcagc agcttcgcgc cggccgcgat gaagctgaag gcgctgtggc ccatgatcgg
    21181 cacgagcacg tagcggccga acagaatcgc cagcccggtg aacgccagga tgagaaaact
    21241 gatggcggtg gtccagtgca gcacgcgttc ccataaggtg aagcgcacga tgcgtcgtcc
    21301 ggtttcgggt tcccgaagac cgataccgcc cttgaacgcg aagaacacca gcgcgccgac
    21361 gaacgcgagc gcgagtgccc agccgctgat ctgcgtcagc ggcccgttgc ggacctgacg
    21421 ccacgtctcg ccttcgctct ggatgagcac accggtttcg cgcccctgta ccgtcgacac
    21481 gcccgcttcg ccggagcgca ctccgcgcca gtacggtgcg ttgttaaagg gttgcgtcgt
    21541 ctgccgctga acctgttctg ccgtgcgatc cgcctgcgcc caggcgccgc ccgatgcgag
    21601 cagcatcaga gcgagcccgc agcacagtat ccagcctatg gtttgctgcc tcatgtcgaa
    21661 ctcctttggg caaagcctga aaaagctttg gggacggccg ccagtgcact ctgctgcacc
    21721 agtacaaaca ggtgatcagc cgcctgtccg atccgacacg tccctatcag ccccattcag
    21781 agcgcctcca ttgccgcgct atcgcgtgcg gctatattcg ttcaggttgg cggcgcgttg
    21841 cgccagttcg cgttcccact tcgcctgatc gccatcatag ggcgccgcct cccatggccg
    21901 gctgtcagtc ttgccggaat agctgcgatc ggtttcgtta taggttgcgg attgatcggg
    21961 ctccgaacag ccggccagca ccagcgcact tgcgacggcg atcattagcg ttctcatagg
    22021 cctcctcatt tcaccgtttc acgaccggcg tcagcagcct gcgccgtttt ctcgggccgg
    22081 ccgtaagcgg tctgccagcc caccatctcc acgcccttgc cgcgcatggt gacgcgctgg
    22141 cggaagatgt ccgacagcac atcgccgtcg ccgccgatca gtgccttggt ggagcacatt
    22201 tcggcacatg ccggcagctt gccttcggcc agacggttcg agccgtactt ggcaaactct
    22261 tcggcactgc ccgtctgctc gggcccgccc gcgcagaacg tgcacttgtc catcttgccg
    22321 cgcatgccga aggcgcccgt ttgcggaaac tgcggcgcgc cgaaaggaca ggcgtagaag
    22381 cagtaaccgc agccgatgca catgtccttg tcgtgcagca ccacgccttc gctggtctga
    22441 tagaagcagt cgaccgggca taccgccata cagggcgcat ccgagcagtg catgcatgcg
    22501 acggaaatgg atttctcgcc cggcacgccc tgattcatcg tcaccactcg ccgccgattg
    22561 acgccccagg gcacttcgtt gacgttcttg cacgcggtca cgcagccgtt gcactcaatg
    22621 cagcgttccg cgtcgcagag gaatttcatt cgagccattg tctatctcct ccgggattcg
    22681 acccgcgcat cgcgcgggcc gtccctcgtt gatggcgttc gtttacgctt tctcgatctg
    22741 gcaaacggtg gttttcgtct cctgcatcat ggtcaccgag tcgtaaccgt aggtcgtggc
    22801 ggtattggcc gcctcaccac ggacaatcgg cgcagcgcct tccgggtagt actccagcag
    22861 atccttgccc aaccaccagc ccgagaacgc gaacggcatg aatgcaaggt tcgcatcgac
    22921 gcgctcagtg accttcgcca tgactttcaa ccgcgccccc tccggcgtgc tcacccatac
    22981 gtattcgccg ttgcgaatgc cgcgatcgtt ggctgcccga ggattgatct ccacgtacat
    23041 attctgttgc agctcggcca accacgggtt ggagcgcgtc tcttcgccgc caccctcgta
    23101 ttccacgatg cggccgctcg tgagcacgat cggaaacttc ttgctgatgt cgcggttctt
    23161 ctgctgcacg gttttgtaca gcgtgggcag ccgccagaac gccttcttgt cgtcgtgggt
    23221 cgggtagtcg tccaccagat ccggtcgcgg gctgtagatc ggctcgcggt gcagcggcac
    23281 cggatcgggg aagttccata ccacggcgcg cgccttcgca ttgccccagg gatgcgagcc
    23341 gtgcttcatg gcgacgcgct ggatgccacc cgagaggtcg gtcttccagt tcttgccctc
    23401 ggcagcctgc ttctcctccg gcgtgaggtc atcccaccag ccgagtttct tcagcagcac
    23461 gtggtcgaac tccggatagc cggtggtgat ctcgtcgccc ttgggatgag agccatcctc
    23521 ggccagcagg ttgacgcctt cccgctccac accgaagttg gcgcggaagc agccgccccc
    23581 gtccatcatc ggcaggctgt tgttgtagag caccggcgag cccgggtgct tgatcgccgc
    23641 ggtaccgtag cagggccacg gcagaccgaa gtagtcgccg tcgagcacat aaccggtttc
    23701 cttgtccacg ccgcccttgg cgcgcagggt cttcacgtcg aatacgtgca tgttgcgcat
    23761 atgcgccttc agccgatccg gcgactggcc ggtgtaaccg atagtccatg aaccccggtt
    23821 gaactccagc gtgatgtcct cgatcaccgg ctcgtcgttg accacctgga tgttctgctt
    23881 gccgttgcgc ttgcccacca gttcgtcggc aaagcccagc ttcttggcaa gcagatacat
    23941 gatggtgtgg tcgggcttcg cctcgaacac cgggtcgatg actttctcgc gccactggat
    24001 cgagcggttc gatgccgtca cggaaccgga ggtttcgaac tgcgttgccg ccggcagcag
    24061 atagacctcg cgattcgggt tgtggtcctc cttgttgccg ggcatcgccg ccatggccgc
    24121 cgtcgcggac ggatagggat cgaccaccac cagcagatcg agccggtcca tcgcgcgctt
    24181 gaagtccaga ccgcgcgact gcgagttggg cgcatggccc cagtagaaga cgccacggat
    24241 gttcggcccc tgatcgatgt actcgttctt ctcgagcaca ccgtcgcacc agcgcgagac
    24301 ggtaattccg ggcttgttca tcatccccgg aacgtagcgc ttcgtcagcc aatccagatc
    24361 gacgttccac acttccgccc aatgcttgaa cgcaccggga gatacgccgt aatagccggg
    24421 caacgaatcc gggttcgggc cgatgtctgt cgcaccctgc acgttgtcgt ggccgcggaa
    24481 gatgttggcg ccgccgcccg ggacaccgat gttgccgagt gccagttgta cgatgcagga
    24541 ggcacgcacc atggcgttgc cgatggagtg ctgcgtctgg cccatgcacc ataccagcgt
    24601 cgagggccgg ttcttcgcca tcgtttcggc cgctttatac agcgccgcct cgggcacgcc
    24661 gctcacacgc tccacttccg ccggcgtcca ggtcttcaac acttcttcgc gcaccttgtc
    24721 catgccgtag acgcgcgcgt tgatgtattc cttgtcttcc cagccgttat tgaagatgtg
    24781 ccagagcaga ccgaacagca ccggcacgtc tgtgccggag cggaagcgga tgtactggtc
    24841 ggccttggcc gccgtccgcg tgaagcgcgg atcgcagacg atgatcttcg cgccggactc
    24901 cttcgcatgc agcgaatgca gcatcgacac cggatgcgcc tccgcggcgt tcgagccgat
    24961 gtagaacatc gccttggagt tgtgcatgtc gttgtaggag ttcgtcatcg caccgtaacc
    25021 ccaggtattc gccacacccg agaccgtggt ggagtgacag atccgcgcct ggtggtcggt
    25081 gttgttggtg ccccacaggc gcacccactt gcacatcagg taggcttgct cgttattgtg
    25141 cttcgacgag ccgatgtagt acatcgcgtc cgggccggac tcttcgcgga tctgcagcat
    25201 cttcttgctg attgcatcga gcgcctcgtc ccagctgacg cgctgatact tgccgttcac
    25261 cagcttcatc ggatacttca gccggtgcga gtgatcggtc atgccgtgtt cgcgcaccga
    25321 cgcgcccttg gcgcagtgcg cgcccaggtt gagcggagaa tcgaacaccg gttcctgccg
    25381 cacccacacg ccgttctcca ccaccgcgtc gatggcgcag ccgaccgaac agtgcgtaca
    25441 gaccgtgcgc ttgacctcga ccttcggcgc gttcgcgggt ttgtcctgcg ccttggcctc
    25501 agtgatcacg ttgaacggca gttgcgtggc gagcgcgccg gcgccgaccg tgacgcccga
    25561 acgcttgagg aaggtgcgcc ggtcgatggt ttcggtcagc gccgcagcaa gcccatgcgc
    25621 tatcccggca cgtgcgccgg ctgtcgtagc ggtgccgctt cttttagtga gtaacatggc
    25681 cgcctccttt agacgagagt ggtgcggtag tacttgcgca cgtgctcgct cagttcataa
    25741 cccttgcgtt cgggcgcttg ctcctgtttc gaggtcgccg ctgacgcggc gttgcctgca
    25801 acggccgccg ctgcggcagc ggccgttccc gccccgacgg acagcatgaa cttcctgcga
    25861 ctcagcttcg gtttggccat gactacctcc tcttgcgatg gattcacagc atctggaacg
    25921 attgctcctc gatgctgaag aacgcctccg tgcacaaagc gacgctctta tagaaatgag
    25981 tctgttgcga atcgcgcgcc gcggcgacga agccccgata gcccggtgcg agataggtgt
    26041 tgaacaattc cgcctggcgc gagattgagt tctcctccgc gccgcccgcc agaatcagat
    26101 gacgcatcac ctcgcatagc gccgcgagat gatcctccgg ttcgtgcgcc gactccgcgc
    26161 gcgccagccc ccactcgtcg agcaggttgc gtaaacgaag caactgccga tccttgcccg
    26221 tcgtgccggg cagccagtgc gccatgtacg gggtgacctc ggccttgccc gtgccgatga
    26281 acaccaggtc gtactcgagt ttcgcgcttt gcaggtccgt ctcgcgggcg gcgcgtgcca
    26341 actgcgccca cgcgcgatcc agcggcgccg ccgcttcgtc gcccgacgcg ggcgcatcgg
    26401 ccatgacgcg caacagcgct tcgtccggcg gcgcatacca gatgcgcgca aggaaggcgt
    26461 accaatccgc ccgcgcctgc gcttcgggat tcaggttgag gttctgcacg cgatcgaggc
    26521 cggtattcat cgcatcattc cttcaccgcc atctcggcgt tctccgccgc ttccatcatg
    26581 tcgatcacgc ggcaatccgc gcacatctgc agccggcgca gcgccggacc gtcgacgaac
    26641 atggaatggt tggtcagccg acccagcatg gtttcgacca cctgcttcgt gccgaaggga
    26701 gtgtggcaac ggatgcagtg gaaaggctgc gcctcgttca gcgtgacttc cttcttcgcg
    26761 tagtcggcaa gcacgagacg cggcgtgagc gacaacgcgt cttccggaca ggtgttcacg
    26821 cacagaccgc attgcacgca gttgcgttcg atgaagcgca acgccggcgt atcgcggccg
    26881 tccaccagcg ccgacgacgg acaggcgccc acgcaggcca gacacagcgt gcacttgtcc
    26941 ttgttcacat tgacccggcc gaacggtgca ccggccgcca gggcgatttc ctccgctggc
    27001 tgcggcgcat gcttcagcag atgttcgaag atgaagtcga gtgcgttgcg cttgttgttg
    27061 gcgagattga aggtggcggt ctgcgcaacg cccgccgcgg gctcgagcgc ccacagcgcc
    27121 tgctccagcg caggcacgcc ctcggcacgc aacaggccga agtgccgtcc ttcatagccg
    27181 aggccgtgca gaatgctttg cgcataagcc aattgccgtt cgagcgcttc gccgtattcg
    27241 tccatctgct gcggcgagag cagcagcatc acctgatgcg cgccgtaggc gatggcgccg
    27301 agcagcaggt cgatcccgat cgacgccgga tgccactgct ccatcggaat gatgcgcgcg
    27361 ggcaacccgc gcccctgccg cgccaggcgc ccgatgtggt cgcgccccgt gctgccgtcg
    27421 tggaacagga tcgctgcggc cttgcccccc gctgcgcggt aaacgctcag catggtcttg
    27481 agccgcgcac cggtatcgct taccgacgga taggcatagc ggatcgcacc ggtcgggcag
    27541 actgtcgtac agccgccgca gccggcgcaa agatgcggct ccacctggat ggaatcgccc
    27601 ttgctcgaaa tggcccaggt cgaacagacg tccatgcaat tgctgcagcc ggtcttcttc
    27661 gagcggctgt gcgcgcagat ccgttcgtcg agcacgaaga acttgggttt gtcgaactcg
    27721 cccaccaggt gcgcgagttg cgcggccgcc cgcccctgcg cgatggcgtc tccgccgggt
    27781 gcgaagtagc cctggggctt atgcggaatc ttgatgagcg gcgcagcatt gagatcgaac
    27841 accagatcga aatccgccgt acgaacggtc ggcgcacgat cgaagtcgat ggcccgcacc
    27901 gcgccgcagg ctttgacgca ttcgcggtgg cccgtgcatt tgttcagatc gatctggtag
    27961 ctgtagtcga tggcctgctc gggacaggcg gcgatacacg cgttgcagcg cgtacagaga
    28021 tcgaggtcga tgggattgcg cggctgccac ttcaacttga acgcgcccag atagccggac
    28081 aggcgcctca cttcaccgga ccagatggca tagcggcgct cgaccggcaa ctccgcaccc
    28141 tgcgcatcgg tgagcagcac gcttacatcg agttggtcgg cgagttgctc ggcccacggc
    28201 aacgcagcga cagccggacc gacaatcagc gtgcgaccgg cggactgata agcgacattg
    28261 ggaaccggct cgggggccgg caattgcgcc gcggcaagga gcgcggcgat tttcggagtg
    28321 gcctgttcgc cttcgaccga ccacccggcg ctttcgcgaa tgttgacgaa acgaagatcg
    28381 gctttgcttt cggctgcatc ggcgacttca gcgaacagcc gcgcttcctg cgtgcaagcg
    28441 accaggcaat cgctgcccgc atccttctgg aatgcaccga tttcgcgacg acacaactga
    28501 tcatggacgg tgagcggaga ctcggtccgt agggcgctcc tcaacgcctc cggatcgagg
    28561 cgcatggtgc cattacagtt gcacagcttc agccgatgga cctgttgcat tgaccgtccc
    28621 gcttaagtgc tatgtcgtac acagcatagc gctttcgcat gtcagagcaa gtgttttcgg
    28681 ttttgggcta actcgcaaaa atggtgccca aggtgatcag gcttgcttcg gttcctcccc
    28741 ggacatttcg tctgcatctt ccacactgtc gtcaaccgcc tcattttttg aggcttcctc
    28801 cggcgtttca gccggcatcg acgaactatc gtcacctccc actggggcgg cttcggatgt
    28861 ggacggctca ctctcttggg cggtcgccgg ttcggaaaaa agcagggacc gcgcttgttc
    28921 cagtccggcc aacatggcgg gcggaatggg gtcggggata ccgtagtcgt cgatatagat
    28981 gtccagcttg tccatctgct cgaagtgaaa atgcggatcg ctgaacagtt tcttcaatgc
    29041 ggccccgcgc gtccgttcgt ccacgcccgc acgcatgaac gcagagtaat cggatgcaat
    29101 gccttgcagg ctgtcgaggg acggcaattc atcggcgtcg tgtccctgcg caggcggctc
    29161 ggcggcgggc acatgcgcat cgacgggcac atgcgcctca atagcaggcc caccatccgc
    29221 ctcggcggcc gcaggcggtg gcgctgcagg cacatccggt atcgattccg tctgcaccgc
    29281 gggctgcgct tcggcggcct gtcgcgcagc agccttgcgc cgcatccagc gtgaaaggaa
    29341 gtgttcttcg tcgctcattc gtcgcgctgc gcccctttga atgaaggtgg ccggctgcgc
    29401 ttcttggggc ccggcttgta gtgcagactg acgaaagccg acacctcgcg caggatttcc
    29461 ttcggcatcg gcacaccctg cacttcctcg ccggaatcca gcaggcgcgc cgcctcgttg
    29521 taactggcag tgactgccca gggcacggcc acgtcgcctt ccaggcgcca ggcgacaaag
    29581 acggccggct cggccgtggt gcagttgaga aaatagccct cggcctcatc tggatataga
    29641 cgcaattcga aaccggcatg caaccagcgc tcgatctgcg cgtcgctgta gtgcagttgc
    29701 ggcgaatcgg ccggcccggc gtccggatcg acggcgatcg gcgtccattg aacgctttgc
    29761 cagcggttgg ccagcagccg ccgctccatc attacgccga ccgtgaagct cgttctgtgc
    29821 atgcccgtgg cttaaaatag cgtctgcgac ccatgatccc ccagccgtaa ggacgaagca
    29881 agccgaacgg ctgcgcacat cggacagtcc aatgcaggaa tgcccgatcc gtgtcgagga
    29941 gatccgcgag tgcgcaaagc ggtacgagcg aggacaatgc aagcgcttgg gcaacacgca
    30001 ctcgcaacgt catcgaccca gcaaccctat tcgaaatcag cgagcctgtc acagcgccaa
    30061 tgaacgaacg gatcattccc atcaccgacg ccggacgcat cccgacgcgc ccggacgagg
    30121 cgcacaccgc gggcaaggcc ctgctcgaca cgcgctcgcg cccgctgcgc gatctgcgca
    30181 tttccgtcac ggaccgttgc aatttccgct gcacctattg catgcccaag gacgtgttcg
    30241 gcaaggatta ccagtttctc gaacacgccg cgctgctcac gttcgaggaa atcacccgcc
    30301 tcgcgcgcgt attcaaggcg cacggcgtgg agaagatccg tctcaccggc ggcgaaccgc
    30361 tgatccgccg caacgtcgaa cgcctcatcg aaatgctggc cggcatcggc ggcctggagc
    30421 tgacgctgac caccaacggg tccctgctcg cgcgcaaggc gcaggcgctc aaggatgcgg
    30481 ggctcgatcg cgtgacggtg agcctcgact cgctggacga cgcgatcttc cagtcgatga
    30541 acgacgtgaa gtttccggtg gcgaaagtgc tggaaggcat cgacgccgcc gcacgtgcgg
    30601 ggctcacccc gctcaaggtg aacgtggtgg taaagcgcgg cgtcaacgat cattcgatcg
    30661 tcgacatggc gcggcatttc aaaggcagcg gccatatcgt acgcttcatc gaattcatgg
    30721 acgtcggcgc caccaacggc tggcgcatgg acgacgtggt cccctcggcc gaagtgatcc
    30781 gccgcatcga tgccgaaatg ccgctcgaac ccgtcggccc gaactatccc ggcgaagtgg
    30841 cgcagcgttg gcgctatgtc gacggcagcg gtgaaatcgg cgtgatctcc tcggtgacgc
    30901 aggcgttttg caacagttgc acgcgcgcac gcctgtccac cgaaggaaag ctctacacct
    30961 gtctgttcgc caacgccggc ttcgatttgc gcgcgctggt gcgcgacggc gcaagcgacg
    31021 aagccatcgc cgacgccatc gccggggtgt ggggcgcgcg cgccgaccgc tactccgaaa
    31081 tccgcaccgc cgagaccgcc cgcgggaaaa aaatcgagat gtcgtacatc ggcggttgag
    31141 tggagagtcg aggactatga aacccgaaat cacccgaacc agccggccgc tgaccttcac
    31201 ggtcgaagcg accaacgaat tgggcgaagt gctgccgacg cccatcgccg gcgaaaatcc
    31261 gctgacgctc tatctggaca agcgcgaaat ccttacgctc atgacgctgg ggcaggcacc
    31321 cgaagcgctg gccatcggtt atctgcgcaa tcaacgactg gtgcgatcca tcgacgaaat
    31381 cgccagcgtg caggtggact gggaaaccga ctcggtcgcg atcaccaccc gccaaggcgt
    31441 cgccgacctc gacagcaaga tggaaaagcg caccgtcacc accggctgcg gtcagggcac
    31501 ggtattcggc gatctgatgg aagagatcga caccatccgt ttgcgcgacg acgtgcagtt
    31561 gcgcgaggac acactgtggg ccgcgctcga cgccgtgcgc aggcgcgaaa ccgtctacaa
    31621 ggcagcgggc gccgtgcacg gctgtgcact gctgaccaac gaggacggcg cgagcgaagt
    31681 catccacttc gtggaagacg tgggccgcca caacgccgtg gacgccatcg ccggtcagat
    31741 gtggctggat gacatcgacg gctccgacaa gatcttctac accaccggcc gcctcacgtc
    31801 ggaaatggtg atcaaggcgg cgcagatgcg cattcccttc ctggtctcgc gctcggggct
    31861 cacgcaaatg gggcatcagg tggcgacgca ggtgggcatg accatgttcg gccgcgccta
    31921 caacaagcgc tatctgctct tcaccggcaa gcaccgcttc gtcaagacga gcatgccgac
    31981 catgctggca tgagtgccgc gaacgacacc accggtctca tcctggccgg cggccagggg
    32041 cgtcgcatgg gtggggtgga caagggcctg caaccgctgc gcgaccggcc gatggtggaa
    32101 tgggtgctcg aacgcctgca gccgcaggtc gccgacgtca tcatcaacgc caaccagaat
    32161 cgcgatcttt acgcgcgcta cggacatccg gtggcgggcg acgagatcgg cggtttcgcc
    32221 ggcccgctcg cgggattgca cgcaggattg aaaagcacct cgcgcgcgtt cgtgctgacc
    32281 gtgccctgcg attcgccttt cctcccgcac gatctgcatg cgcgcctgta tgccgcctcg
    32341 caggcggcgg atgccgatct cgccgtggcg aaaacgggcg atcaggcgca tccggtgttc
    32401 gcgctggtgc gcacccgggt gctgccgcat ctggaagcct ttctgcaagg cggcggccgc
    32461 aagatcgacg cgtggtatgc cacgctcaag gtcgtggaag tgcccttcga cgacgaagcc
    32521 gacgccttca gcaacatcaa tacgcgcgac gagttgcgcg cgcacgaaca gcgcgcctga
    32581 acagcgcttc aatggaccga gccatgaacg acatcaagaa aatcatcagc tgcatggacg
    32641 actacgatcc ggacgcgctc gtcgtaccga aggcccacga ggcgatccgc gcctgtctca
    32701 agccgatcag cggtgccgaa cgcgtcaacg tccgcgcaag cctcggccgc gtgctggcgg
    32761 aggacatcat ctcgcccatc aacgtacccg cgcataacaa ctcggcgatg gacggctggg
    32821 cgctgcgcgg cgccgacctg gccgccgacg gcgagacgcg tctgcgcgaa gtgggcgtcg
    32881 cctatgcggg ccgcgcctat gccggcaccg tcaagcaggg cgaatgcgta cgcatcatga
    32941 ccggcgcggt catgcccgaa ggtgcggaca cggtggtcat ccaggaagtg gtgcgcagcg
    33001 agggtgacag catcatcgtc ccgcccggcg aaaagccggg acagaacgtg cgcatggccg
    33061 gcgaagacct cagcgagggc aaaccggtgc tacgcgccgg acagcaggtg cgtccggccg
    33121 aactgggtct gatggcttcg ctcggcatgg cggaagtcgt cgtctatcgc cgcctgcggg
    33181 tggccttctt ctccaccggc gacgagctcg cctcggtcgg ctcggtgctc aaggaaggcg
    33241 aggtgtacga ctccaaccgc tacaccctgt acggcatgct ccaacgcatg ggttgcgatc
    33301 tgctcgacat gggcgtggtg cgcgacgatc ccgacagcct cgaacgcgcc ttccgcgaag
    33361 ctgccgcgaa tgccgacgtg atcatcactt ccggcggcgt atcggtgggc gaagcggatt
    33421 acacgcgcga tctgctcggc cgcatcggcg aagtgctgtt ctggaagatc gccatgaaac
    33481 cgggccgccc catggccttc ggcaagatcg ataacgcgta cttcttcggc ctgccgggca
    33541 acccggtggc ggtgatggtc actttctatc agttcgtgcg caatgccctg ctgttcctct
    33601 ccggccggac cggcgagacg gaactgcccc tgctgcgcgt gccctgtacc acggcgatga
    33661 gaaagcgtcc gggccgcacg gaattccagc gcggcattct ctcgcgcgac gcccaaggcc
    33721 agtggcaggt cgcggtgagc ggcgcacagg gctcgggcgt gctgcgctcc atgtcggaag
    33781 ccaactgctt catcgtactg gaacacgatc gcggcaatgt gcagccgggc gatctggtca
    33841 gcgtgcaggt aatggaggga atggtttaag aagaacagtc gagagctgag agtcgagagc
    33901 gagagaatcc gggcgaggct tccttcaaaa aatctcgcac catatggcac agagaaaccc
    33961 ccagttccaa tgctttcttc gtgttgtccg cgctcttcga tggtctccgt gtttcaggct
    34021 tgctcgacgc ccctgcccgc cacttcagcc atgcgcgcgc aggccaatgc cagcgtcacg
    34081 ggatccacgg gcttggcgat gtgcatctgg aagcccgcag acagcgcccg gtagcgatcg
    34141 tccgcgcgcg cgaaagcggt cagcgcaatc gccggcgtac gcccgcccag ctcaggccgc
    34201 aactcgcgga tccggcgcat cagcgcatag ccgtcggtgt tcggcatgcc gatgtcgctg
    34261 atgatgaggg cgtagcgctc ctgcgccaga cacgccatcg cctcgtcggc gctggccgca
    34321 acactcacct ccgcgtcgta ctccgccagc atgcggcgca acagttcacg cgcatccggt
    34381 tcgtcgtcca ccaccagtat tttcaggccg gacagtgaag gcgcatccgg cgacgggaca
    34441 tccgcttcgg tggcaccggc gccgtcgtcc gccgcgatct ccgggggcat ggccgcgacg
    34501 gcgggaatcg gcagcagcac ggtgaacgtt gcgccctgcc cttcgccggg actctccgca
    34561 cgaatcgagc cgccgtgcag ctccaccagg ctcttgacga tggacagacc caggccgagc
    34621 ccgccgtgcg cgcgcgtggt cgaactgtcg cgctgccgga agcggtcgaa cacctgcggc
    34681 aggaactccg ccgacatgcc cacgccgttg tccgcgacct gcagcatcag tgcgctatcg
    34741 gcacgaccca gccgcacggt gacttcgccg cccgacggcg taaatttgat ggcattggtc
    34801 aacagattcc acagcacctg ctgcagacga ttgggatcgg catttacggc aggcacgtcg
    34861 cgctcgactt ccagcgccag gcggacgtgc ttggcatccg ccatcggccg taccacgtcc
    34921 agcgcggcat cgaccacctc gggcagccgc accggctgga tttccagtcg caccttgccg
    34981 gagacgatgc gactcatgtc cagcagatcc tcgatcaact gctgctgcgc cttggtgttc
    35041 cgctcgatgg cctcgagcgc ggcggccgta tccgccgcat tcatgacccg ccggcgcagc
    35101 agttgcgccc agcccagcac cgcactcagc ggcgtgcgca gttcgtggct gagcgtggcg
    35161 aggaactcgt ccttcatgcg caccacgcgc tcggcctctg cacgcgcagc acgctcggca
    35221 ttgatgatct gttcgcgttc gcgctcgatg cgcttggcat cggtgatgtc gcgcgtgacg
    35281 cccagatgcg cgagaccctc gtcggccatg gtgatcggca cggcatgggt ttccatccaa
    35341 tagcgcgtgc cgtccaggcc gacgatctcg aactgccaca ccaactgctc cccggcgcag
    35401 acgcgttcgt ggttcgtctg ccacgtcgcc cggcgccgcg gcacgatgaa attcaccagc
    35461 ggctgcccgc gcaccgcatc cagcgattcc gcgcccagca tggccaggcc cgcattgttg
    35521 atgtactcga tggtgccgtc ccgccggatg atcttgacga actccggggt cgtctcgacc
    35581 acggcgcgaa agcgcgcttc gctgtcgcgc agattgcgcg cgacgcgcgc gcgctcgatc
    35641 gcttcccagc agcgcgaagc gaccagacgg acgagttcga tttcgtcgcg agtccatgcg
    35701 cgcggctgca tctgatggag cgccagtgcg gcgaccagcc tcccctgctt gaccagcggc
    35761 gcgcagacga tggccctgat ccccaaccgg tcgaaagcgt tgtccgccga tgtcggatcg
    35821 ctctgacgaa ggtccgcgct ggcaaaggtt tgacccgcat gcaatgctcg cccgaactgc
    35881 tcgccgaagt cgcgcaggcg ataacgcccc accgcaccgt cttcgcgctg cgtgcggtcg
    35941 cccaccacga cgaaggcgtc ctcgtcggca tcgaactggg cataggcgca acgatccgcc
    36001 tccaggtgtt gccgcagcat gcgcgccgca agcgtcgtga t
//
LOCUS       DATGOU010000072         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3744492, whole genome shotgun sequence.
ACCESSION   DATGOU010000072 DATGOU010000000
VERSION     DATGOU010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3744492"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VKZ48_04345"
     CDS             <1..457
                     /locus_tag="VKZ48_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HLU77121.1"
                     /translation="VPSLNLPFVGTDPSISRVWLALSCAAAAVIGHVWPVWHNFRGGK
                     GAATLIGTLIVLAPSLILPVLLVWAWILTLFGYAGLASITAALFAPLYLALTRLPDDQ
                     PLFIYSLVLSLFLVYCHRSNIQRMLAGTESRNTHLMLFRKREHADRGND"
     gene            450..1265
                     /locus_tag="VKZ48_04350"
     CDS             450..1265
                     /locus_tag="VKZ48_04350"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HLU77122.1"
                     /translation="MTDPDDILQRIGSEARASLELLEVFPELESTNTWLLQQASPSRG
                     RFRAAIAEHQTAGRGRSDKIWLSPSGSGLCLSLAYTFAELPRHLPSLTLAVGAAIATA
                     LENLGAKGLALKWPNDLMAQDGKLGGILTELQSKGEGGRAVVVGLGLNVDLPDRMRYM
                     PPASWTSKFTDLAACLPELPERAALIAAVIRSIVECIERFAREGFGHFHSIWQRYDWL
                     KGRRWSVQQAQRRITGVAEGIDEEGALLLRTNAGLERVISGSIELPAYGAAIG"
     gene            1262..2032
                     /locus_tag="VKZ48_04355"
     CDS             1262..2032
                     /locus_tag="VKZ48_04355"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HLU77123.1"
                     /translation="MNLVRALLFDVGNTRLKWGLLAQGRIGKSGSIAHEKLHDSGFGA
                     LTANLPRDIDTVLVSNVAGQTFATRLSGVIGMHCNCEVHFARAEREGYGLTNSYRRPR
                     RIGVDRWVAMIGAYAEFRSALCVVDAGTAVTIDAVDRDGVHLGGMIVPGLSMMADALH
                     VNASEIPLAKRNLAATADAMNAFANTTEGAVQNGALTAISGAIERSVRLMRGAGFRPK
                     VVLTGGDASRILKLLDANVLHRPNLVLEGLAFMVQSDK"
BASE COUNT          368 a          632 c          693 g          372 t
ORIGIN      
        1 cgtgccgtca ctgaacttgc cgttcgtcgg caccgatccc tcgatttcgc gcgtgtggct
       61 tgccttgagt tgcgcagcgg cagcggttat cgggcacgtc tggcctgtgt ggcacaactt
      121 tcgcggcggc aagggagcgg caacactcat cggcacgctg atcgtgctgg cgccgagcct
      181 gatcctgccg gtgctgctgg tgtgggcatg gatcctgacg ctgttcggct acgccggcct
      241 ggcgtccata acggcggcgc tcttcgcacc gctgtatctc gcgctgacgc gtctgccgga
      301 tgaccagccg ctcttcatat acagcctcgt gctgtcgctg ttcctggttt actgccatcg
      361 ctccaacatc cagcgcatgc tggccggcac ggagtcgagg aatacgcacc tcatgttatt
      421 tcgcaagcgg gaacatgccg atcgtggcaa tgactgatcc cgacgacatc ctgcaaagga
      481 tcggtagcga ggcgcgcgca tcgctcgagt tgctggaggt gtttccggaa ctggaatcga
      541 ccaatacctg gctgctgcaa caagcgagcc cgagccgcgg gcggtttcgc gcggcgatcg
      601 ccgaacacca gacggccggc cgcggccgca gcgacaagat ctggctgtcg ccgagcggat
      661 ccggactttg tctttcgctc gcgtacactt tcgccgagct gccgcggcac ttgcccagcc
      721 tgacgctggc ggtcggcgct gccatcgcga ccgcactgga gaacctcggc gcgaaaggcc
      781 tggcgctgaa atggccgaac gacctcatgg cgcaggacgg caagctcgga ggaatcctga
      841 cggaactgca gtcgaagggt gaaggcggcc gcgcggtggt cgtcgggctt ggattgaacg
      901 tggacctgcc ggacaggatg cgctacatgc cgccggcctc gtggaccagc aagtttacgg
      961 atcttgccgc atgcctgccg gagttgccgg agcgagccgc gctaatcgca gccgtgatca
     1021 ggagcatcgt cgagtgcatc gagcgcttcg cgcgcgaggg tttcggtcac ttccattcca
     1081 tatggcagcg ctacgactgg ctcaagggca ggcggtggag tgtgcagcag gcgcaacgcc
     1141 gcattaccgg cgtcgcggaa ggaatcgacg aggaaggcgc actactcctc aggacgaacg
     1201 cgggactgga gcgcgtcatc tccggctcca tcgagcttcc ggcctacgga gccgccatcg
     1261 gatgaatcta gtgcgggcgt tgctgttcga cgtcggaaac acgcgcctca aatggggcct
     1321 gctggcgcag gggcgcatcg gcaagagcgg cagcatcgcc cacgagaagc tgcacgactc
     1381 gggtttcggc gcgctgacgg ccaacctgcc gagggacatc gacactgtcc tggtgagcaa
     1441 tgttgccggc cagacatttg cgacgcgcct gtcgggcgtc atcggcatgc actgcaactg
     1501 cgaggtgcac ttcgcgcgcg cggaacgcga aggctacggg cttacgaaca gctaccggcg
     1561 accgcgcagg atcggcgtgg accggtgggt ggcgatgatc ggtgcctatg ccgagtttcg
     1621 ctcggcgttg tgcgtagtgg atgccggcac ggccgtgacc atcgacgccg tggatcggga
     1681 cggagtgcac ctgggcggca tgatcgttcc cggtctctcg atgatggcgg acgcgttgca
     1741 cgtaaacgcc agcgagatcc cgctggcgaa aagaaacctg gccgcaacgg cggatgcgat
     1801 gaacgcattc gccaacacaa ccgaaggcgc agtgcagaat ggtgcgctga cggcgatcag
     1861 cggtgccatc gaacgctcgg ttcgattgat gcgcggagcg ggcttccggc cgaaagtcgt
     1921 cttgaccggc ggcgatgcgt cgcgcatcct gaagctcctg gacgcaaatg tgctgcaccg
     1981 gcccaatctc gtgttggagg gccttgcgtt catggtacag agcgataaat gaaaaacctg
     2041 ctgctgctac tcgtcctcgc gaaca
//
LOCUS       DATGOU010000073         6695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_5073684, whole genome shotgun sequence.
ACCESSION   DATGOU010000073 DATGOU010000000
VERSION     DATGOU010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6695
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5073684"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1502)
                     /gene="gatB"
                     /locus_tag="VKZ48_04360"
     CDS             complement(42..1502)
                     /gene="gatB"
                     /locus_tag="VKZ48_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020206853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HLU77124.1"
                     /translation="MNWEVVIGLETHAQLSTVSKIFSGASTAFGAAPNTQASAVDIAL
                     PGVLPVLNKRAVEYAIRFGLAIGAKVNRRSIFARKNYFYPDLPKGYQISQYELPIVEG
                     GEMTIVTSAGEKTIRLTRAHLEEDAGKSLHEDFQGMSGIDLNRAGTPLLEIVSEPDMR
                     SAEEAVAYAKALHALVRWIDICDGNMQEGSFRCDANVSVRRKGDPEFGTRREIKNLNS
                     FRFLQQAIEYEVRWQIETLEDGGKIEQATVLFDPATGETRTMRTKEDAHDYRYFPDPD
                     LPPLAVSEEWIEHVRAGLPELPQAMCERYQREFGLSAYDARTLTQSREMAAYFEDLAS
                     RLNGVQDAAAAKLAANWVMGELAAALNRNDLSIEAAPVSAAQLARLLTRIRDNTISGK
                     IARDVFDAIWAGEAGGEDAADVLIERKGLKQISDVGALTAIVEEIIAANPKSVEEFRG
                     GKDKALNALVGQAMKATKGKGNPAQLAELFRAKLNG"
     gene            complement(1627..3081)
                     /gene="gatA"
                     /locus_tag="VKZ48_04365"
     CDS             complement(1627..3081)
                     /gene="gatA"
                     /locus_tag="VKZ48_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012214358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HLU77125.1"
                     /translation="MLNASLAELSALLAAKKVSSVELTQACLARIDALNPRLNAFVTV
                     DPERSLAQARAADERIARGEAAPLTGIPVAHKDIWCAAGWRTTCGSRMLENFIAPYSA
                     HVIERFDRAGAVNLGKCNMDEFAMGSSNETSYFGPVKNPWNTDCVPGGSSGGSAATVA
                     ARLAPAATGTDTGGSIRQPAALCGVSGLKPTYGLVSRYGMVAFASSLDQGGPFARSAE
                     DLALLLGVMAGFDERDSTSLERPPENYSRLLSNPLAGLRIGLPREYFGEGLADDVRAA
                     VDAALAELRKQGATTVDIELPNSALSVPAYYVIAPAEASSNLSRFDGVRYGYRAPEYA
                     DLADMYSKTRGQGFGEEVKRRILVGTYVLSHGYYDAYYLQAQRVRRLIADDFRAAFSQ
                     CDVIMGPTTPTAAFKLGEKVSDPVQMYLNDIYTIAANLAGLPAMSIPCGFSSAGLPVG
                     LHLVGNYFDEARMLNVAHRYQQATDWHTRMPDID"
     gene            complement(3111..3398)
                     /gene="gatC"
                     /locus_tag="VKZ48_04370"
     CDS             complement(3111..3398)
                     /gene="gatC"
                     /locus_tag="VKZ48_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HLU77126.1"
                     /translation="MPLSAEEIKRIARLARIEVTDDELEGVRTRLNGIFAMIEQMQAA
                     PTEGVVPMAHAQDVVLRLREDAVTESDQHRLFQSIAPQVEADLYLVPKVIE"
     gene            3518..4561
                     /locus_tag="VKZ48_04375"
     CDS             3518..4561
                     /locus_tag="VKZ48_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004628293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HLU77127.1"
                     /translation="MLGFLRAYFSDDLAIDLGTANTLIYVRGKALVLDEPSVVAIRQE
                     GGPNGKRIIQAVGKEAKQMLGKAPGNITVIRPMKDGVIADFTVTEQMLKQFIKKVHVS
                     RLLSPSPRIIICVPCGSTQVERRAIRESAIGAGASQVYLIEEPMAAAIGADLPVAEAE
                     GSMVVDIGGGTTEVGIISLGGMVYAGSVRVGGDKFDEAIINYIRRNYGMLIGEATAEM
                     IKKDIGSAFPGSEVKEIEVKGRNLAEGIPRSFTISSNEILEALTEPLNQIVSAVKTGL
                     EQTPPELGADIAEKGMVLTGGGALLRDLDRLLMEETGLPVIVAEDPLTCVVRGSGKAL
                     EKMDKLGSIFTNE"
     gene            4625..5548
                     /gene="mreC"
                     /locus_tag="VKZ48_04380"
     CDS             4625..5548
                     /gene="mreC"
                     /locus_tag="VKZ48_04380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HLU77128.1"
                     /translation="MEYQPPQFFKRGPAPLARLTFFGLLSVLLMVLDARFQYAEPLRH
                     GLTLAIYPLQQVATAPVVAFNRVSDYFSSQTRLRRENAELRAQQLENAQSLLVLESIA
                     NENVQLRKLLDARQPLPSPAVFAEIVYAGRDPFSRKVIIDRGSQHGIEAGQPVVDGEG
                     VLGQVTRVLPLLAEVTLVTDKNHSIPVQVLRNGLRGVAYGSGDGNTLELRFIPTNAEI
                     QTGDVLVTSGLDGIYPRGLPVATVTSVERDTSASFAKIRLAPAAGTNKRRQVLVVQYR
                     ENLPTRAEAETKFRAAESKETKRPARTEGRQ"
     gene            5545..6066
                     /gene="mreD"
                     /locus_tag="VKZ48_04385"
     CDS             5545..6066
                     /gene="mreD"
                     /locus_tag="VKZ48_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004189260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HLU77129.1"
                     /translation="MRGNQGPQRILRPANPFFIVFTLLGALAFNLLPWPDLRLVPDLL
                     ALTLTFWCIHQPRRVGVGVAWVLGLAMDAANGALIGQHALAYALLAFGAHVIHRRVLW
                     FPVWPQAVHVFALLLLSQLVMLAVGLIAGGTFPGWLVLVGIGLSAVLWPFITDLLLAP
                     QRAPDDIDETRPI"
     gene            6221..>6695
                     /locus_tag="VKZ48_04390"
     CDS             6221..>6695
                     /locus_tag="VKZ48_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HLU77130.1"
                     /translation="MSFTEFRDHQRDLHRFRLRLIVAGVAVFAAFGLLLGRFVWLQYI
                     HHEYYSTKAESNRISVVPIQPNRGLIMDRNGVVLARNFSAYTLEINPDQIDDLNATIQ
                     ALSDVVEIEERDLNRFRKLRNEFRVADTLPIKTRLTDEEVARFAANAYRFPGVEIK"
BASE COUNT         1189 a         2169 c         2124 g         1213 t
ORIGIN      
        1 tcaccagcga cgcccgctga acgcttcgcc gcagacgccg atcagccgtt gagcttcgcg
       61 cggaacaact cggccagttg cgccggattg cccttgccct tggtcgcttt catcgcctgg
      121 ccgaccagcg cgttcagcgc cttgtcctta ccgccgcgaa attcctccac cgacttcggg
      181 ttggcggcga tgatttcctc cacgatggcg gtcagcgcac ctacgtcgct gatctgcttg
      241 aggcccttgc gctcgatcag cacgtcggcc gcatcctcgc ccccggcctc gcccgcccag
      301 atggcgtcga acacgtcccg tgcgatcttg cccgagatgg tgttgtcgcg aatgcgcgtg
      361 agcagccgcg caagctgcgc ggcggagacc ggcgccgcct cgatcgacaa gtcgttgcga
      421 ttgagcgccg cggccaactc tcccatgacc cagttggcgg cgagcttcgc cgcggcggca
      481 tcctgcaccc cgttcagacg cgaggcgagg tcctcgaaat aggccgccat ttcgcgcgac
      541 tgcgtcagcg tgcgcgcatc gtaagccgac aacccgaact cccgctgata gcgctcgcac
      601 atggcctgcg gcaactcggg caacccggcg cgcacgtgct cgatccattc ctcgctcacc
      661 gccagcggcg gcaggtccgg atcgggaaaa tagcggtaat cgtgcgcgtc ttccttggtg
      721 cgcatggtgc gcgtctcgcc ggtcgcgggg tcgaacagca cggtggcctg ttcgatcttg
      781 ccgccgtcct ccagcgtttc gatctgccac cgcacttcgt actcgatcgc ctgctgcagg
      841 aagcggaagg agttgaggtt cttgatttct cggcgcgtgc cgaactcggg atcacccttg
      901 cgtcgcacgg agacgttggc gtcgcagcgg aacgagccct cctgcatgtt gccgtcgcag
      961 atatcgatcc agcgcaccag cgcatgcagc gccttggcgt aggcaaccgc ctcttccgcc
     1021 gaacgcatat ccggttcgga cacgatttcc agcagcggcg taccggcacg gttcaaatcg
     1081 atgcccgaca tgccctggaa atcctcatgc agcgacttgc cggcatcctc ttccaaatgg
     1141 gcgcgcgtca ggcgaatggt tttttcgccg gcggaggtga cgatggtcat ctcgccgccc
     1201 tcgacgatgg gcagttcgta ctggctgatc tggtagccct tgggcaggtc gggatagaaa
     1261 tagtttttgc gcgcgaagat ggagcgccgg tttaccttgg cgccgatggc caggccgaaa
     1321 cggatcgcgt attcgactgc gcgcttgttc aataccggca gcacgccggg cagcgcgata
     1381 tcgaccgccg aagcctgcgt gttgggcgcc gcgccgaagg ccgtcgaagc gccggagaaa
     1441 attttcgaga cggtggaaag ctgcgcgtgc gtttccagac cgatgacgac ttcccagttc
     1501 ataaactcta gcctttcacc accaaggcac aaagaacacc aaggcgcacc aagaaaacca
     1561 acccgaatct tggtgtcttg gtgtcttggt ggttaaagat tttcatgcgc cgccgatacc
     1621 gtctgtttaa tcgatgtccg gcatgcgcgt gtgccagtcg gtggcctgct gataacggtg
     1681 ggccacattc agcatgcgcg cctcgtcgaa gtagttgccc accagatgca accccaccgg
     1741 cagccccgcg ctgctgaagc cgcagggaat cgacatggcg ggcaggccgg ccaggttggc
     1801 ggcgatggtg tagatgtcgt tgaggtacat ctgcaccggg tcggacactt tttcaccaag
     1861 cttgaacgcc gcggtcggcg tggtcgggcc cataatcacg tcgcactgac tgaaagcggc
     1921 ccggaaatcg tccgcgatca gacggcgcac gcgctgcgcc tgcagatagt aggcgtcgta
     1981 atagccgtgc gagagcacat aggtgccgac cagaatgcgg cgcttcactt cctcgccgaa
     2041 accctgcccg cgcgtcttgc tgtacatgtc ggcaagatcg gcgtactcgg gtgcgcggta
     2101 gccgtagcgc acgccgtcga aacgcgagag attggaggag gcctccgccg gcgcgatgac
     2161 atagtaggcc ggcaccgaca gcgcactgtt gggcagttcg atatcgacgg tcgtcgcacc
     2221 ctgcttgcgc agctcggcca gtgcggcgtc gaccgccgcg cgaacgtcgt cggcgaggcc
     2281 ctcgccgaag tattcgcgcg gcagcccgat gcgcagcccg gcaagcggat tgcttagcaa
     2341 gcgcgagtaa ttttcgggcg gacgttcgag actggtggaa tcgcgctcat cgaagccggc
     2401 catgacgccg agcagcaacg cgagatcctc ggcactgcgg gcgaaagggc cgccttgatc
     2461 gagactggaa gcgaaagcga ccatgccgta gcgggacacg aggccgtagg tcggcttgag
     2521 acccgacacg ccgcacagcg cggccggctg acggatggat ccaccggtgt cggtgccggt
     2581 tgccgccggc gcgaggcgcg cggcgaccgt cgcggccgat ccgcccgagg agccgcccgg
     2641 cacacagtcg gtattccagg ggtttttcac cgggccgaaa taggaggttt cgttggacga
     2701 acccatggcg aactcgtcca tgttgcactt gccgagattc accgcgcccg cccggtcgaa
     2761 gcgttcgatc acatgcgcgc tgtacggcgc gatgaagttt tccagcatgc gcgagccgca
     2821 ggtggtacgc cagccggcgg cgcaccagat gtccttgtgc gcgacgggaa tgccggtgag
     2881 gggtgcggct tcgccgcgcg caatacgctc gtcggccgcg cgcgcctggg ccaggctgcg
     2941 ctcgggatcg acggtaacga atgcgttgag tcgcggattg agcgcgtcga tgcgcgccag
     3001 gcacgcctgt gtcagttcga cactggaaac cttttttgcg gccagaagcg cagagagctc
     3061 ggccagggaa gcattcagca tgatgtggag gcggggcgcc ggacgcccgc ttattcaatg
     3121 actttgggaa cgagatacag atcggcctcg acctgcggcg cgatggattg gaacagccgg
     3181 tgctgatcgg attcggtgac cgcatcctca cggaggcgca gaacgacatc ctgggcatgc
     3241 gccatgggca ccacgccttc ggtaggggcg gcctgcatct gctcgatcat ggcgaagatg
     3301 ccgttgagcc gcgtgcgcac gccttccagt tcgtcatcgg tgacttcgat ccgggcgagc
     3361 cgggcgatcc gcttgatttc ttcggcgctt agtggcattt gcaaatttcc gctcaaaaca
     3421 agcgtttaaa ctcatgtatt atacgtcgcc tttcgaagcc agaatggggc tctctcacta
     3481 ttcaggctga ttcgcgacgc taatcggacg ctctccaatg ctgggatttc tacgcgcata
     3541 cttttccgac gatctggcca tcgacctcgg cacggccaat acgctgatct acgtccgcgg
     3601 caaggcgctc gtcctcgacg agccctccgt cgtcgcaatc cgccaggaag gcggcccgaa
     3661 cggcaagcgc attattcagg cggtgggcaa ggaagccaag cagatgctgg gcaaggcgcc
     3721 gggcaacatc accgtcatcc gcccgatgaa ggacggcgtc atcgccgact tcaccgtcac
     3781 cgagcagatg ctcaagcagt tcattaagaa agtgcacgtc tcgcgcctgc tgtcacccag
     3841 cccgcgcatc atcatctgtg tcccctgcgg ttcgacccag gtggagcgtc gcgccatccg
     3901 cgagtcggcc atcggcgcag gcgcctcgca ggtttatctg atcgaagagc ccatggcggc
     3961 tgccatcggc gccgacctgc cggtggcgga agccgaaggt tcgatggtgg tagacatcgg
     4021 cggcggcacg acggaagtgg gcatcatctc gctgggcggt atggtctatg ccggctccgt
     4081 gcgcgtgggc ggcgacaagt tcgacgaggc gatcatcaac tacatccgcc gcaactacgg
     4141 catgctgatc ggcgaagcca ccgccgaaat gatcaagaaa gatatcggct cggccttccc
     4201 cggctccgaa gtgaaggaga tcgaggtcaa gggccgcaac ctcgccgagg gcattccgcg
     4261 cagcttcacg atttcgtcca acgaaatcct cgaagcgctg accgagccgc tcaaccagat
     4321 cgtcagcgcg gtgaagaccg ggctggagca gacgccgccc gaactgggtg cggatatcgc
     4381 cgagaagggc atggttctca ccggcggcgg ggcgctgctg cgtgacctgg accgactgct
     4441 gatggaggaa accggcctgc cggtcatcgt cgccgaggac ccgctgacct gcgtggtgcg
     4501 cggctccggc aaggcactcg agaagatgga caagcttggc agcatcttca ccaacgaata
     4561 aaccctgttg cccggcatcc ggcatgccgg tgtcgggtaa cacctgacgg gatccttttg
     4621 ccgcatggag taccagccgc cccagttttt caagcgcggc cccgctccgc ttgcgcggct
     4681 cacgttcttc ggtctgttgt cggtcctgct gatggtgctc gacgcgcgct ttcagtatgc
     4741 cgagccgctg cgccacggcc tgacgctggc gatctacccg ctgcagcagg tggcgactgc
     4801 ccccgtggtc gccttcaatc gcgtcagcga ttacttcagc agccagaccc gtctgcgtcg
     4861 cgagaacgcc gaattgcgcg cccagcaatt ggagaacgcg cagtcgttgc tggtgctgga
     4921 gtcgatcgcc aacgaaaacg tgcagttgcg caaactgctc gacgcgcggc agccgctacc
     4981 ctcgcctgcc gtgttcgccg aaatcgtcta tgccgggcgc gacccgttca gccgcaaagt
     5041 catcatcgac cgcggcagtc agcacggcat cgaagcgggg cagccggtgg tggacggtga
     5101 gggcgtgctg ggtcaggtga cgcgcgttct gccgctgctg gccgaagtca cgctggtcac
     5161 cgacaagaac cactccattc cggtgcaggt gttgcgcaac ggtctgcgcg gcgtcgccta
     5221 cggttcgggc gacggcaata cgctcgaact gcgcttcatc ccgaccaacg ccgagatcca
     5281 gacgggcgac gtgctcgtca cctccggcct ggacggcatt tatccgcgcg gcctgccggt
     5341 cgccaccgtc accagcgtgg agcgcgacac cagcgcctcc ttcgccaaaa tccgcctggc
     5401 gcccgcagcc ggcaccaaca agcgtcgcca ggtgctggtg gtgcagtatc gggagaacct
     5461 cccgacgcgc gccgaagccg agacgaaatt ccgcgcggcc gaaagcaagg agacgaagcg
     5521 gcctgccagg acggagggca ggcaatgagg ggcaaccagg gaccgcagcg catcctgcga
     5581 ccggccaatc cttttttcat cgtcttcacc ctgctcggcg cgctggcctt caatctgctg
     5641 ccctggcccg atctgcgcct ggtgcccgat ctgctggcgc tcacgcttac cttctggtgc
     5701 atccatcagc cgcgccgggt gggcgtcggc gtggcctggg tgctgggcct ggcgatggat
     5761 gccgccaacg gcgcgctgat cggccagcac gcgctcgcgt atgcgctgct cgccttcggc
     5821 gcgcacgtca ttcaccggcg cgtgctgtgg ttccccgtct ggccgcaggc ggtgcacgtg
     5881 ttcgcgctgc tgctgttgag ccaactcgtc atgctggcgg tgggtttgat cgccggcggc
     5941 acttttccgg gctggctggt gctggtcggt atcggcttgt ccgcggtgct ctggccgttc
     6001 ataacagact tattgctcgc tccgcagcgc gcacccgacg atatcgacga aactcgtccg
     6061 atctagcggg ctctcatgac cgcagccgat gccggcgcat cgtgccgccc gaaggagatg
     6121 cagccgtgcg aacaccggcg aagcggcgct ttggcaaccg gcggcgatta aaaaaacgac
     6181 ctgatcagag cagaacgata gagacacacc cggccgattc atgagcttta ccgaattccg
     6241 cgatcaccag cgcgatttac accggtttcg cctgcgactg attgtcgccg gcgtcgccgt
     6301 gttcgccgcg ttcgggctgc tgctgggccg cttcgtatgg ctgcagtaca tccaccacga
     6361 gtactactcg accaaggcgg agagcaaccg catttccgtc gtgcccatcc agcccaatcg
     6421 cggcctgatc atggaccgca acggcgtggt gctggcgcgc aacttctcgg cctacacgct
     6481 cgaaatcaat ccggaccaga tcgacgacct gaatgcgacc attcaggcgc tgtccgacgt
     6541 ggtggaaatc gaggagcgcg atctcaaccg cttccgcaag ctgcgcaacg agtttcgcgt
     6601 cgccgatacc ctgccgatca aaacgcgcct gaccgacgag gaggtggcgc gcttcgccgc
     6661 caatgcgtat cgctttcccg gcgtggagat caagg
//
LOCUS       DATGOU010000074         4136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_1980292, whole genome shotgun sequence.
ACCESSION   DATGOU010000074 DATGOU010000000
VERSION     DATGOU010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1980292"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..697)
                     /gene="flgA"
                     /locus_tag="VKZ48_04395"
     CDS             complement(14..697)
                     /gene="flgA"
                     /locus_tag="VKZ48_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HLU77131.1"
                     /translation="MHAYTYRLFFALSLAVAGFAPTAHALDEAQLRDFVLNEVHAGAG
                     RVEVGIDRLAAQLERAPCRRLEPFLSSGARLWGRSTVGVRCADGTRWSTYVPIDVRIY
                     GPALVAARDLNAGETPAPHDVRIEEVELTREPPGLLTDTMQLEHMLVQRTLRAGQPIR
                     TSHLRARPVVTSGALVKLTYSGAGFVVSAEGRALAPAAEGQTVRVQTDSGKVLTGVAR
                     AGGIVEIRS"
     gene            858..1268
                     /gene="flgB"
                     /locus_tag="VKZ48_04400"
     CDS             858..1268
                     /gene="flgB"
                     /locus_tag="VKZ48_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HLU77132.1"
                     /translation="MIGKLDQFMHLQGEALKLRAQRQEVIASNLANADTPGYKAVDFK
                     FSDALRQAVGMPAPTGAAPALLRTDAKHLGPQGSTLSTVQLMYRTPTQASVDGNTVDM
                     NIEQANFADNAVRYEASLRFLNGKIKTMLAAIQG"
     gene            1296..1727
                     /gene="flgC"
                     /locus_tag="VKZ48_04405"
     CDS             1296..1727
                     /gene="flgC"
                     /locus_tag="VKZ48_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HLU77133.1"
                     /translation="MSLAKVFEISGSAISAQSQRLNVVSSNLANAESVTGPDGKAYRA
                     RQVVFQTLPVGDPNAAQSAQGATTAGVKVAGIVEDQSPMRQVYNPQHPLADEKGYVEM
                     PNVNVVEEMVNMISASRSYQTNVEVMNTAKSLLLKTLSLGS"
     gene            1738..2403
                     /locus_tag="VKZ48_04410"
     CDS             1738..2403
                     /locus_tag="VKZ48_04410"
                     /inference="COORDINATES: protein motif:HMM:NF025229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HLU77134.1"
                     /translation="MAVGGVDAGNPLAQYQNTAAKSKSATDDIQDRFMTLLITQMKNQ
                     DPLNPLDNAQVTSQLAQLNTVKGIEQLNETVGMLMGRMQNSETLAGIGAVGRLALIEG
                     EKIALYEGQAAAGFELPEDAEQLKVKIYDSVGNVLHEAELGAHPAGLHTFVWDGMTDS
                     GQAAVNGNYRFEIIARNGEDVVKATPLSIGRIDGIVPNQDQLTFTIGGMPQTKLSDIK
                     QFL"
     gene            2447..3664
                     /gene="flgE"
                     /locus_tag="VKZ48_04415"
     CDS             2447..3664
                     /gene="flgE"
                     /locus_tag="VKZ48_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014630919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HLU77135.1"
                     /translation="MSFQQGLSGLNAASKNLDAIGNNVANSNTVGYKSASTTFADVYA
                     TSLAGGGATGIGIGVAVASVAQNFSQGNIIGTSNPLDMAINGNGFFRLSRDGEIVYSR
                     NGQFHLDRDGYIVNATGANLTGLQATNGLIGGAVTNLRVQVDGAGGFPQASTAASIIA
                     NLDAEAGVIDQTIYPFDPTDPATYTNATSMTLYDDIGVSRTVATYFVKTAPNTWEVYA
                     TEDGALVAPQPPAAPAGRIGALTFAAGRLDAATLAALPFNVNGVTLDFEGSTQVASGF
                     GVTELWTDGYPPGRFSGLTISDDGIIQTRYSNGRTEIVGQIRLDDFTNPHGLQPLGNN
                     LFAETPESGQPIPGTPMTGRAGSVNSGALEESNVDLTAELVNMITAQRVYQANAQTIK
                     TQDQIMQTLVNLR"
     gene            3880..>4136
                     /locus_tag="VKZ48_04420"
     CDS             3880..>4136
                     /locus_tag="VKZ48_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HLU77136.1"
                     /translation="MDKIIYTAMTGAKQAMARQDTLAHNLANVNTTGYRADTTAFRAV
                     PIRGAGEGTRVFTLESTPGVDLSSGPVRQTGRELDVSLSEGG"
BASE COUNT          832 a         1342 c         1299 g          663 t
ORIGIN      
        1 attctttagc gcattaagac cttatttcga caatgccgcc ggctcgagcc actccggtca
       61 gcaccttgcc cgagtcggtc tgcacccgga cggtctgacc ttccgccgcc ggcgccaggg
      121 cacgaccctc ggccgaaacc acgaagcccg ccccgctgta ggtcagcttg accagggcgc
      181 cggaagttac caccgggcgc gcgcgcaaat gactggtgcg gatgggctgg cctgcccgca
      241 gggtgcgctg caccagcatg tgctccaact gcatcgtgtc ggtcagcagg cccggcggct
      301 cgcgggtcaa ctccacttcc tcgatgcgca cgtcatgcgg cgccggcgtc tcgccggcat
      361 tgagatccct tgcggcaacc agggccggac cataaatgcg cacatcgatc gggacatagg
      421 tgctccagcg cgtgccgtcg gcacagcgca cgccgacggt gctgcggccc cacagtcgtg
      481 cgccgctgga tagaaacggc tccagacgcc ggcagggcgc gcgctccaac tgcgccgcca
      541 gccggtcgat gccgacctcg acccggccgg caccggcgtg cacctcgttc agcacgaagt
      601 cgcgcaattg cgcttcgtcg agcgcatgcg ccgtcggggc gaaacccgct acggcgagac
      661 tcaatgcgaa aaacaggcgg tacgtgtacg catgcatggc atcgttggga cgctttacaa
      721 gtgagtgcaa gattaatgag gacccatgca agccaagcgg cgaatagccg tgcaaaagcc
      781 gcctttcttg tccgattggg gtagggcggc ctgcccataa ttgcgccatc gaggaaacca
      841 gtcccatggg agggcgcatg atcggcaagc tcgaccagtt catgcatttg cagggcgaag
      901 ccttgaagct gcgtgcgcaa aggcaggagg tgatcgcctc caatctcgcc aacgccgata
      961 cgccgggata caaggcggtg gatttcaaat tctccgacgc gctgcgtcag gcggtgggca
     1021 tgcctgcgcc gacgggcgcg gcgcccgctt tgctgcgcac tgatgcgaag cacttgggtc
     1081 cgcagggctc cacgctgtcc acggtgcagc tcatgtaccg cacgcccacg caggcgagcg
     1141 tggacggcaa cacggtggac atgaacatcg aacaggcgaa cttcgccgac aacgccgtgc
     1201 gctacgaggc ttcgctgcgc ttcctcaacg gcaagatcaa aaccatgctt gcggccatcc
     1261 agggctgagt gaagcgtttc aagggaagga tccacatgtc cttagccaaa gtattcgaga
     1321 tttccggttc ggccatttcc gcgcaaagcc agcgtctcaa tgtggtgtcg agcaacctcg
     1381 ccaacgccga gagcgtgacc ggcccggacg gcaaggccta tcgcgcccgt caggtggtgt
     1441 tccagacgct gcccgtgggc gatccgaacg cggcgcagtc ggcgcagggt gcgaccaccg
     1501 caggcgtgaa agtcgccggc atcgtcgaag accagagtcc gatgcgccag gtctacaacc
     1561 cgcagcatcc gctggccgac gaaaagggct atgtggaaat gcccaacgtc aatgtggtgg
     1621 aggagatggt caacatgatc tccgcctcgc gttcctacca gaccaacgtc gaagtcatga
     1681 acacggccaa gagcctgttg ctcaagacct tgtcgctggg ctcataagga gagaccgatg
     1741 gctgtaggag gcgtggacgc cggcaatccg ctggcgcaat accagaacac cgcggcgaag
     1801 agcaaaagcg cgaccgacga catccaggac cggttcatga cgctgctcat cacgcaaatg
     1861 aagaatcagg acccgctcaa tccgctggac aacgcgcagg tcacctcgca gcttgcacag
     1921 ttgaacacgg tgaaaggcat cgaacagctc aacgaaaccg tcggcatgct gatgggccgc
     1981 atgcagaaca gcgaaacgct ggccggcatc ggcgcagtcg gccggctcgc gctgatcgaa
     2041 ggcgaaaaga tcgcgctcta cgagggacag gcggcggccg gcttcgagtt gccggaggac
     2101 gcggagcagc tcaaggtgaa gatctacgac agcgtgggga acgtgctgca cgaagcggag
     2161 ctcggcgcgc atccggccgg actgcatacc ttcgtgtggg acggcatgac cgattcgggc
     2221 caggccgcgg tcaacggcaa ctaccgtttc gaaatcattg cgcggaacgg cgaggacgtc
     2281 gtcaaggcga cgccgctgtc catcggccgc atcgacggca tcgtgccgaa ccaggaccaa
     2341 ctgactttca ccatcggcgg catgccgcag accaagttgt ccgacatcaa acaatttctc
     2401 taacgggcgg cgcatcgcgc ttcgctaacg cagacggaga accagcatga gcttccagca
     2461 aggcctttca ggactgaacg ccgcgtcgaa aaaccttgac gccatcggca acaacgtcgc
     2521 caactcgaac acggtgggct acaagagcgc ctccaccacc tttgcggatg tctatgcgac
     2581 ttcgctggcc ggcggcggcg cgacgggaat cggcatcggt gtggcggtcg cctccgtggc
     2641 gcagaacttc agccagggca acatcatcgg cacctccaat ccgctcgata tggcgatcaa
     2701 cggcaacggc ttcttccgtc tttcgcgcga cggcgagatc gtctactcgc gcaacggcca
     2761 gttccatctg gaccgcgacg gctacatcgt caacgccacc ggcgcgaacc tcaccggtct
     2821 gcaggccacc aacggtttga tcggcggcgc cgtgaccaat ctgcgcgtac aggtggatgg
     2881 tgcgggcggc ttcccgcagg cgtccactgc ggcgtccatc atcgccaacc tcgatgccga
     2941 ggccggcgtg atcgatcaga ccatctaccc gttcgatccc accgatcccg cgacctacac
     3001 caacgccacc tcgatgacgc tgtacgacga tatcggcgtc tcgcggaccg tggcgaccta
     3061 tttcgtgaag acggcgccga acacctggga ggtgtacgcg acggaggatg gggcgctcgt
     3121 ggcgccgcag ccgcctgcgg cgccggcagg acgcatcggc gcgcttacgt tcgcggcagg
     3181 tcgtctcgat gccgcgacgc tggccgcact gccgttcaac gtcaacggcg tgacgctcga
     3241 tttcgagggc tccacgcaag tggcttccgg tttcggcgtg accgaactgt ggacggacgg
     3301 ttatccgccc ggccgcttca gcggcctgac catcagcgac gacggcatca tccagacgcg
     3361 ctactccaac ggccgcacgg aaatcgtcgg ccagatccgc ctggacgatt tcaccaatcc
     3421 gcacggtctg cagccgctgg gcaacaacct gttcgccgag acgcccgagt cgggccagcc
     3481 gattcccggc acgccgatga ccggccgtgc gggttcggtc aattccggtg cgctggagga
     3541 atccaacgtc gacctcactg cggaactggt caacatgatc accgcgcagc gcgtgtatca
     3601 ggcgaacgcg cagaccatca agacgcagga tcagatcatg cagacgctcg tcaacttgcg
     3661 ttgacgcact tagtggagag tgaagagtgg agagtgggga gtcataagag agcgtgatta
     3721 acgttcgtcc tgagccgcgc gcgtagcgcg cgagtcgaag gatgaacgcg cgtcgggctc
     3781 aaacggcgcg ccggttcatg gttcgactgc tcgctgcgct cgcgctcacc acgaacggcg
     3841 ttgtctgctc tcgactctcg gctctccgcc aggagcaata tggacaaaat catctacacc
     3901 gcgatgaccg gcgccaagca ggccatggcg cggcaggaca cgctggcgca caacctcgcc
     3961 aacgtgaaca ccaccggcta ccgtgccgat acgacggcgt tccgcgccgt accgatccgc
     4021 ggtgcgggcg agggcacgcg cgtgttcacg ctggaatcga cgcccggtgt cgatctgtcc
     4081 tccggtccgg tgcggcagac ggggcgcgaa ctcgatgtgt cgctgtccga aggcgg
//
LOCUS       DATGOU010000075         9711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_4642635, whole genome shotgun sequence.
ACCESSION   DATGOU010000075 DATGOU010000000
VERSION     DATGOU010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9711
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4642635"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..502)
                     /locus_tag="VKZ48_04425"
     CDS             complement(188..502)
                     /locus_tag="VKZ48_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLU77137.1"
                     /translation="MATKKLADSERGAYRKLEDVIGCKWSVSVLQSVAAGIDRPGAME
                     RHIEGISTKVLSERLRKLCNYELLTRHVYAEVPPRTEYTLTEKGRKLVKIIEEIKALD
                     QE"
     gene            635..871
                     /locus_tag="VKZ48_04430"
     CDS             635..871
                     /locus_tag="VKZ48_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HLU77138.1"
                     /translation="MAGSAVFYHAGCPVCVEAEQTVAHAIDPAKYRLEIVHLGEDKGR
                     LKEAEAAGVVSVPALVLDGVPFHINYGAALSALK"
     gene            1086..1532
                     /locus_tag="VKZ48_04435"
     CDS             1086..1532
                     /locus_tag="VKZ48_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HLU77139.1"
                     /translation="MSATMDRRAQVHVYSVHAIHPFHAVMLAATLPLFLGALLSDWAY
                     WRTHEIQWANFSSWLIAGALVFGAVALLFAVFGLFARRGRGGRRSLYLLLLLAMWVLG
                     FINALAHAKDAWAVMPMGLLLSVIVALLAIAATWVGFSTLRSEMVS"
     gene            1529..2830
                     /locus_tag="VKZ48_04440"
     CDS             1529..2830
                     /locus_tag="VKZ48_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HLU77140.1"
                     /translation="MRRATLSTIAAVALALMLGACTEKGGEQQTYGPDPQLPEPNRGL
                     LPRMVIAKPAPWGDKAPTVPQGYTVTAIATDLGIPRQSLVLPNGDILVAEGRGGADAP
                     KLKPKDEIAGVIKAKGTTQVDSGHRVRLLRDADGDGQYELKTVFADDLNAPYGLAYHD
                     GKLYVANQDSLVRFDYRDGQTKAEGPPHKLTDLPSAINHHWTKSMAISPDGRFLYVGI
                     GSNSNITERGMTAEVDRAVVWQIDAESGMHKPYATGLRNPTALAIQPDTDVLWAVVNE
                     RDEIGPDLVPDYLTHVQEGGFYGWPYAYWGPNADTRVMPSDPEKVKATIKPDYALGSH
                     VAALGVAFSAPSMGGQFADGVFVGEHGSWNRNPPVGYRVVFVPFRDGKPAGDPIDFVT
                     GFREDDGTTYGRPVGVTVDPRGALIICDDLANIVWRVTPAR"
     gene            2955..3095
                     /locus_tag="VKZ48_04445"
     CDS             2955..3095
                     /locus_tag="VKZ48_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77141.1"
                     /translation="MNLLHTPDGRYFIERGPPWWQDGAPDFNRHLVKNTPYAAWYRAQ
                     HA"
     gene            complement(3137..3802)
                     /locus_tag="VKZ48_04450"
     CDS             complement(3137..3802)
                     /locus_tag="VKZ48_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77142.1"
                     /translation="MNVNTTVKQAPARAAEIVREYGPFKGFDAVHGVTHDGNSVWAAG
                     GAKLVAIDPQSGEIARTLERAGDAGTAFDGKHLYQIAGKRIDKIDLASGDVVASIPAP
                     GQGSDSGLAWAEGSLWVGAYRERKIHQVDPESGKVLRTIESNRFVTGVTWVDGELWHG
                     TWEGDESDIRRIDPQSGEVFERLRMPEGVGVSGLESDGAQLFYCGGGQSGKVRVVRRP
                     GAA"
     gene            complement(3854..5074)
                     /locus_tag="VKZ48_04455"
     CDS             complement(3854..5074)
                     /locus_tag="VKZ48_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLU77143.1"
                     /translation="MDSLVAAAGRALAVGDALTALKHVGLRNDPPALALRGIAMAQLD
                     DLPRARSLLRRAARAFGANEALARARCVVAEAEVAFAMRDFSGSPRTLLAAAAALEAR
                     GDIANALHARLIAVRRMLLLGRLADAEVALATLDGQALPPPLYAIAELAAAELALRSL
                     HTAEAAAALTRAQAAAERARIPALIAEVDEMRAVPNRPAARRVDAAQAQSLRLDEVEA
                     LIDSGALVIDACRRQVRAVDRVRSLARRPVLFELVRTLGESWPGDVDREVLIERAFNL
                     RQPDETHRARLRVEIGRLRALLKPIVRIEATERGFKLAPLDGREVVVLMPPIDGDQAS
                     LVALLADGQAWSTSALALALGASQRTVQRALVALQEAGQVRAIGRARAQRWLAPPLAG
                     FTTILLLPAALPDA"
     gene            complement(5167..5550)
                     /locus_tag="VKZ48_04460"
     CDS             complement(5167..5550)
                     /locus_tag="VKZ48_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLU77144.1"
                     /translation="MARHLEETPDRFMTGCPSREALRIIAGKWALLVIPALKDGPVRN
                     NELLRRIDGISQKVLTECLKELERNGLVIRTDFNANPPHVEYRLSPLGRSLSDALLPL
                     DQWVNANSVALEDARKIFDETRAGR"
     gene            5644..5967
                     /locus_tag="VKZ48_04465"
     CDS             5644..5967
                     /locus_tag="VKZ48_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013396691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HLU77145.1"
                     /translation="MAWSLLIGAGVLEIVMAVALRFSAGWSKPIPSALGVAAAMASVF
                     LLTHALKFLPTGVAYAIWTGMGSVGVALIGIFFLGEQASVMRMACIALIVAGMIGLRL
                     SDPIV"
     gene            5976..6596
                     /locus_tag="VKZ48_04470"
                     /pseudo
     CDS             5976..6596
                     /locus_tag="VKZ48_04470"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            complement(6625..7218)
                     /locus_tag="VKZ48_04475"
     CDS             complement(6625..7218)
                     /locus_tag="VKZ48_04475"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HLU77146.1"
                     /translation="MDAPFLGGHPAVDFLNTRFTPDDGAVEAIGDGKAFVDWLVVAEL
                     LDETTASSLVRRFGVKALDAAAEEARRFRERTRAWLARWRDVPEDDYRAEVRALNKLL
                     ERLAVRWEVVDGSGMTLVQRPCIETAEALVGLVAAQVAALIAHEDPALLKECAGSGCS
                     LWFLDRTKAHRRKFCTAAVCGNRAKVSAFRERQRAGS"
     gene            7333..8352
                     /locus_tag="VKZ48_04480"
     CDS             7333..8352
                     /locus_tag="VKZ48_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLU77147.1"
                     /translation="MKPRPTAPLQAAAGLTTSAGSSAIPTAVLNAAGSSTPSGRVETS
                     ATRYRTVQVDGLDIFYREAGPKDAPVLLLLHGFPASSFMYRDLMTQLADRYRVIAPDY
                     PGFGYSAAPSAKEYSYTFDRLADVIEKFTAQLALHRYAIYMQDFGGPVGFRLATRHPD
                     NVTALIVQNANAYEEGLPEKFWALPKSVWRDPSPEKLRVFREEVVSDAALEWNYTHGV
                     RDQTFINPDNWVLQQALVNRPGNKDIMLALLYDYRTNPEQYPAWQAYFREHQPPTLIV
                     WGKHDEIFPAPGAEAYLKDLPDAELHLLDTGHFALEDKSREIAQYMRPFLSKLVQQPS
                     RRAAS"
     gene            8617..8820
                     /locus_tag="VKZ48_04485"
     CDS             8617..8820
                     /locus_tag="VKZ48_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HLU77148.1"
                     /translation="MAIGTVKWFNDQKGFGFISPEGGGEDLFAHFSAITGGGFKTLQE
                     NQRVEYDATRGPKGMQASNIRPL"
     gene            complement(8926..9063)
                     /locus_tag="VKZ48_04490"
     CDS             complement(8926..9063)
                     /locus_tag="VKZ48_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77149.1"
                     /translation="MSPWLPIGIACGVAIGAMYGNIAVGIVIGLILGVALESIERTGR
                     K"
     gene            complement(9072..9500)
                     /locus_tag="VKZ48_04495"
     CDS             complement(9072..9500)
                     /locus_tag="VKZ48_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLU77150.1"
                     /translation="MEVNELHRGRLIDHIQLVVKDLTAARKFYNAVLSVLDIPVQGEG
                     EGFFWADEFVVSAADSPAALGMLTGRNHIAFQARDRAAVDAFHKAALAAGGKDNGEPG
                     ERPYHPGYYAAFVLDPDGNNIEAVYHGEAKRSASSVHITF"
BASE COUNT         1688 a         3202 c         3070 g         1751 t
ORIGIN      
        1 tcaaacgaat ggggcgggtc ctccatacaa cataagacgc ccggcccgct ccttatgtga
       61 cgccttcatg cgatcaatcc gtcttttttg tcacaggcgg cgcatcccgg ccgtcttact
      121 gatcggacaa gctcgcccgt ctgcggctcg caacggcaac cgcgcgatcg tggcggcaag
      181 acagacgcta ctcctgatcc agcgccttga tctcctcgat gatcttcacc agcttgcgcc
      241 ccttttccgt cagcgtgtat tcggtgcgcg gcggcacttc cgcatagacg tgcctggtca
      301 gcagttcgta gttgcacagc ttgcgcaggc gttcgctcag gaccttggtc gagatgcctt
      361 cgatgtggcg ctccatggcg cccggacgat cgatgcccgc tgcgaccgac tgcaacaccg
      421 agacggacca cttgcagccg atcacgtctt ccagcttccg gtaggcaccg cgctcactgt
      481 ccgcaagttt ttttgtggcc atgcttccgc aacttacccc gataagtaag tagggcaccg
      541 aaaagtgccc gctgttcaag ccgaggctcg cttcctacat tgcgctcgag cgagtcgaat
      601 gtcgcggctc gtactatagc gaaaggagtg cgtcatggca ggcagtgcag tgttctatca
      661 cgcgggttgt ccggtatgcg tcgaggctga gcaaaccgtc gcgcatgcca tcgatccggc
      721 gaagtatcgg ctcgagatcg tacatctcgg ggaagacaag ggccggctga aggaagcgga
      781 agcggcgggc gtggtttcgg tgcccgcgct ggtgctggac ggcgtgcctt ttcatatcaa
      841 ctacggcgcg gcgctgtccg cgttgaagta gccccctcgc gcctcgtcgt tcgcggcgag
      901 gcgcgaccac cctcctcctt acgcgttgtg ccccggtgcg gcgtgcaggc aggcgtgcac
      961 gttgcaagcc gaattcgacc gacaccgcga tcaggctctg ctgtgcgggc accttctcag
     1021 gcgctcagca catcgactcc gtggttcgag gttccggttt aaccgctcaa ccatcaggag
     1081 gaaccatgtc cgccacgatg gaccggcgtg cgcaagtgca cgtctattct gtccatgcca
     1141 ttcacccctt tcatgcggtc atgctggccg cgaccctgcc gctgtttctc ggcgcgctgc
     1201 tgagcgactg ggcgtactgg cgcacgcatg agatccagtg ggcgaacttt tcgtcctggc
     1261 tgatcgccgg cgcactggtg ttcggcgccg tcgcattgct gttcgcggtg ttcggactgt
     1321 tcgcccggcg cggacgcggc ggacggcggt cgctctatct gctgctgctc ctggcgatgt
     1381 gggtgctggg cttcatcaat gcgctggccc atgccaagga tgcctgggcg gtgatgccga
     1441 tgggactgtt gctgtcggtg atcgttgcgc tccttgcgat tgcagcaacg tgggtggggt
     1501 tctccacgct tcggtcggag atggtgtcat gaggcgcgcg accctatcaa cgattgccgc
     1561 ggtggcgctc gcgctcatgc tcggcgcctg caccgaaaaa gggggcgagc agcagaccta
     1621 cggccccgat ccgcaactac ccgagcccaa ccgcggcttg ctcccccgca tggtgatcgc
     1681 aaaacctgcg ccgtggggcg acaaggcgcc gactgtgccg cagggctaca ccgtcaccgc
     1741 catcgccacc gatctgggca ttccgcgcca gagcctggtg ctgcccaatg gcgatattct
     1801 cgtggccgaa ggccgcggcg gcgccgacgc gccgaagctg aagccgaagg acgagatcgc
     1861 cggcgtcatc aaagccaagg gcaccacgca ggtggacagc ggccaccggg tgaggctgct
     1921 gcgcgatgcc gacggcgacg gtcagtacga gttgaagacg gtcttcgccg acgacctcaa
     1981 tgcgccttac gggctcgcct atcacgacgg caagctctac gtcgccaatc aggattcgct
     2041 ggtgcgcttc gactaccgcg acggccagac aaaggccgag ggaccgcccc acaagctcac
     2101 cgatctgcct tcggcgatca accatcactg gacgaaatcg atggccatca gcccggacgg
     2161 ccgctttctg tacgtcggca tcggctcgaa cagcaacatc accgagcgcg gcatgaccgc
     2221 cgaagtcgat cgcgcggtgg tgtggcagat cgatgcggaa tccggcatgc acaagcccta
     2281 cgccaccggc ctgcgcaacc ccacggcgct ggcaatacag ccggacacgg acgtgttgtg
     2341 ggcggtggtg aacgaacgcg acgagatcgg ccccgacctc gtgcccgact atctgacgca
     2401 cgtgcaggaa ggcggcttct acggctggcc ctatgcctat tggggcccga acgccgatac
     2461 gcgcgtgatg ccgtccgatc ccgagaaagt gaaagcgacc atcaagccgg actacgcgct
     2521 gggctcgcat gtcgccgcgc tgggcgtggc gttctcggcg ccgtcgatgg gcgggcagtt
     2581 cgccgacggc gtgttcgtgg gcgaacacgg cagttggaac cgcaacccgc cggtcggcta
     2641 cagggtggtt ttcgtgccgt tccgcgacgg caagccggcc ggcgatccga tcgacttcgt
     2701 caccggtttt cgcgaggacg acggcacgac ttacggccgg cccgtcggcg tgacggtgga
     2761 cccgcgcggc gcgctgatca tctgcgacga tctggcgaac atcgtgtggc gggtgacgcc
     2821 ggcgcggtga gcggtagtgc cggggctgag tcgacaccgc ccgttcgtcc cgagccgcgc
     2881 acagcgcgag tcgaaccctg tcctgagccc gacgaaggga atgaacgggc gcagacgcac
     2941 tgcaacgttg aagcatgaac cttcttcaca ccccggacgg ccgctacttc atcgagcgcg
     3001 gcccgccctg gtggcaggac ggcgcgccgg acttcaatcg tcatctggtg aagaacacgc
     3061 cctatgcggc gtggtatcgc gcacagcacg cttgagtcga ggcccggcga acgcccgaag
     3121 ctcgccgggc aatggctcag gccgcaccgg ggcgacgcac gacacgcact ttgccgctct
     3181 gtccgccgcc gcaatagaag agttgcgcgc cgtcggattc gagcccgctg acgccgacgc
     3241 cttcgggcat ccgcaagcgc tcgaacacct cgccgctttg cggatcgatg cggcggatgt
     3301 cgctttcgtc gccttcccac gtgccgtgcc acaactcgcc gtccacccag gtcacgcccg
     3361 tgacgaagcg gttggattcg atggtgcgca gcaccttgcc cgactcggga tcgacctggt
     3421 ggatcttgcg ctcgcgatac gcgccgaccc acaggctgcc ttccgcccag gcgagacccg
     3481 agtcgctgcc ctgaccgggc gcggggatgg aggcgacgac gtcgccgctg gccagatcga
     3541 tcttgtcgat gcgcttgccg gcgatctgat aaagatgctt gccgtcgaag gcggtgccgg
     3601 cgtcgcccgc gcgttcgagt gtgcgtgcga tctcgccgct ttgcggatcg atggcgacga
     3661 gcttcgcgcc cccggcggcc cacacgctgt tgccgtcatg ggtgacgcca tgaacggcgt
     3721 cgaagccctt gaacggcccg tattcgcgca cgatttcggc tgcgcgcgca ggggcctgct
     3781 ttacggtcgt gttgacattc atttgcagct cctttcggtc cggcgcgcag gatcgcaacg
     3841 ccgtccgtgc aatctacgca tccggcagcg cagcggggag taacaagatt gtcgtgaatc
     3901 ccgcaagcgg cggcgcgagc caacgctgcg cgcgcgcacg gccgatggcg cgcacctgcc
     3961 cggcctcctg caacgccacg agtgcgcgct gaacggtccg ctgactggcg ccgagcgcca
     4021 atgcgagtgc ggaggtggac catgcctggc cgtcggcgag cagcgccaca agcgaagcct
     4081 gatcgccgtc gatgggcggc atgagtacga cgacctcgcg cccatccagc ggtgcaagtt
     4141 tgaagccccg ttcagtcgct tcgatacgca cgatcggttt gagcagcgca cgcagccggc
     4201 caatctccac gcgtagccgc gcgcgatgcg tttcatccgg ctgccgcaga ttgaatgcgc
     4261 gctcgatcaa cacttcgcga tcgacatcgc cgggccagct ttcgccgagc gtgcgtacca
     4321 gttcgaacag cacgggccgg cgtgcaagcg atcgcaccct atcgactgcg cgcacctgtc
     4381 gacggcaggc gtcgatgacc aacgcgccgg aatcgatcag cgcttccact tcgtcgaggc
     4441 gcagcgattg cgcctgtgcc gcatcgacgc gccgtgcggc cgggcggttc ggcacggcgc
     4501 gcatctcgtc gacttcggcg atgagcgccg gaatgcgtgc gcgctcggcc gctgcttgcg
     4561 cgcgggtgag cgctgccgcc gcttcggccg tgtgcagcga tcgcaatgca agctcggccg
     4621 cggccagttc cgcgatggcg tagagcggcg ggggcaaagc ctgtccgtcg agtgttgcga
     4681 gcgcgacctc ggcatccgcc aaccgcccga gcagcagcat gcggcgcacg gcgatgagtc
     4741 gcgcatgcag ggcgttggca atgtcgccgc gcgcttcgag cgccgctgcg gccgcgagca
     4801 gtgtgcgcgg cgagccgctg aagtcgcgca tcgcgaacgc cacctcggct tccgcgacga
     4861 cgcagcgtgc gcgcgcaagc gcttcgttcg cgccgaaagc gcgcgccgcg cgccgcaaca
     4921 agctgcgcgc acgcggcaag tcgtccagtt gcgccatcgc gatgccgcgc aacgcgagcg
     4981 caggcggatc gttgcgcaag ccgacatgtt tcagcgcggt gagcgcatcg cctacggcca
     5041 gtgcccgccc ggcggcggca acgagggagt ccatgggcgc ggagggtacg acgtgaaggc
     5101 gtgtagtgcg aggggcaagt tcggtcggct aaggcagcaa cgggcggccg gtcgctgacg
     5161 ggcaactcat cgccccgcgc gtgtttcgtc gaagatcttg cgcgcatcct caagcgccac
     5221 gctgttcgca ttgacccatt gatcgagcgg aagcagcgcg tcgctcaacg agcgtccgag
     5281 cgggctcaag cggtattcga catgcggcgg attggcattg aagtcggtgc ggatcaccag
     5341 gccgttgcgc tcgagttcct tcagacattc cgtcagcacc ttctgcgaaa tgccgtcgat
     5401 gcgacgcagc agttcgttgt tgcgcaccgg cccgtccttc aacgcgggaa tgacgagcag
     5461 cgcccacttg cccgcgatga ttcgcagcgc ctcgcgcgac ggacagccgg tcatgaaacg
     5521 atcgggtgtt tcttcaaggt ggcgcgccat ggtcgcctcc acggttactt aaaagtgcgt
     5581 acttgtgcgg cagcgcaacg atccttagct tagcgcctcg attcaccacc aggaagtgcc
     5641 atcatggctt ggagtctatt gatcggcgcg ggcgtgctcg agattgtcat ggccgtcgca
     5701 ctgcgttttt cagcgggatg gagcaagccg attccgagcg cgctcggggt cgccgccgca
     5761 atggcgagcg tgtttctgct cacgcatgcg ctcaagttcc tgcccaccgg tgtcgcctat
     5821 gcgatctgga cgggcatggg ctcggtcggc gtcgcgctga tcggcatctt ctttctcggc
     5881 gagcaggcgt cggtgatgcg catggcgtgc attgcgctga tcgtggcggg catgatcggc
     5941 ctgcgcctat ccgacccgat cgtgtaggag gcgacatgga acggctgctc tacatcgaag
     6001 cctcgcccac gaaacaaagc tcgacctcga ttgcagtcac gcgcacgttt ctggatgcct
     6061 atcgggcttc gcgtgccgtc gtggtggaca gactggatct ctgggattgc gacctgccgc
     6121 ctttcgacgc ggagatgatc gaagcgaagt tcgccgtgct ccgcgcccgg aacgcgacgc
     6181 ccgcacagca ggcgaaatgg gcgcaggcgg tcgcgctgtc gcagcgtttc aatgcggcgg
     6241 acaagtatgt gttcagcctg ccgatgtgga atttcggcat tccgtatcgt ttgaagcact
     6301 tcatcgacgt cgtcaccctg cccggctaga actggacgtg gtcgcgcgca gaaggctacc
     6361 ggccgctgct gcgcaacaag aaggcgctgc tcgtgtatac cagcgcgggg gactatcccg
     6421 tcggacccga ctacgccgca gaggatttcc agaagccgta catgcggcag tggctgcggt
     6481 tcatcggcgt gaccgacctg agcgaggtcg ccgttgcgcc gacgctaacg tcgccggagg
     6541 cattgcaaag catcaagaca cgggcggtcc ggcagttggc cgagctgagc ttttgatggc
     6601 ccgggcgcgc cggatcgcgt gcgactaact tccggcgcgc tgacgctctc tgaacgccga
     6661 caccttggcg cgattgccgc acaccgccgc ggtgcagaac ttgcggcgat gcgccttggt
     6721 gcgatccaga aaccaaaggc tgcagccgga ccccgcgcac tccttgagta gcgccggatc
     6781 ctcatgcgcg atcaatgccg cgacctgcgc ggcaaccaga cccacgagtg cttccgccgt
     6841 ctcgatgcag ggacgttgaa ccagcgtcat ccccgacccg tcgaccacct cccaccgcac
     6901 cgcgagacgc tcgagcagct tgttcaaggc gcggacttcg gcgcgatagt cgtcctcggg
     6961 cacgtcgcgc cagcgggcga gccacgcgcg ggttcgttcg cgaaagcggc gcgcttcttc
     7021 cgcagccgca tccagagcct tgacgccaaa gcgccgaacc agcgatgagg ccgtcgtctc
     7081 atccagcaat tcagccacga ccagccagtc cacaaaggcc ttgccgtcgc cgatcgcttc
     7141 caccgccccg tcgtccggcg taaagcgcgt attgagaaag tcgaccgccg ggtggccgcc
     7201 tagaaaggga gcatccatga ccggaactgt aaccccttaa aaagctcttg acaagttacc
     7261 ctccccggct tccaatgtaa ccgccaattg acgtgtttat tggttaccac tcaaccttac
     7321 ccggagaaac ccatgaagcc ccgccctacc gcccccttgc aggccgccgc cggcctaacg
     7381 acatccgccg gctcatccgc aatcccgacc gctgtgctca acgccgccgg cagctccacg
     7441 ccgagcgggc gcgtggagac ttccgccacc cggtatcgca ctgtgcaggt ggacggcctc
     7501 gatatcttct atcgcgaggc cggcccgaaa gacgcgcccg tccttttgct gctgcacggc
     7561 tttccggcat catcgttcat gtatcgcgac ctcatgacgc aactggccga ccgctatcgc
     7621 gtgatcgcgc ccgactatcc cggcttcggt tacagcgccg caccttccgc caaggaatat
     7681 tcgtacacct tcgatcgtct ggcggacgtc atcgagaagt tcaccgcaca actggcgctg
     7741 catcggtacg ccatctacat gcaggatttc ggcggaccgg tcggatttcg cctcgcgacc
     7801 aggcacccgg acaacgtcac ggcgctcatc gtgcagaacg cgaacgccta cgaggagggt
     7861 ttgccggaga agttctgggc gctgcccaag tccgtctggc gcgatccgtc gccggaaaag
     7921 ctgcgagtgt tccgcgagga agtcgtttcg gacgcggcgc tcgaatggaa ctacacccat
     7981 ggcgtgcggg accagacctt catcaacccg gacaactggg tgctgcagca agcgctggtc
     8041 aatcgcccgg gcaacaagga catcatgctg gcgctgctct atgactaccg cacaaacccc
     8101 gaacagtacc cggcctggca ggcttacttc cgcgagcacc agccgccgac cctgatcgtt
     8161 tggggcaagc atgacgaaat ctttccggcc ccgggggcgg aggcgtatct gaaagatctg
     8221 cccgacgccg aactgcatct attggacaca ggccacttcg cgctggagga caagagccgc
     8281 gagatcgctc agtacatgcg cccatttctg agcaagctcg tacaacagcc gtcgcgacgc
     8341 gccgcgtcgt aacgtgcctt cgcccttgac cgtacgccac ctcggtggcg tacggaaaaa
     8401 gcgcgggcgc cgcggtagac aaaataatcg gcgccgaaac acgattgttg ttgaccgccc
     8461 cctatatacc gcgtacggtg cgcgggcagg ttgcgacggc ggttctcgcc acagaatgta
     8521 cccctggttg attccctctt ctggtctgga tgttttgagc gcttgcaggc ggggttcgcc
     8581 ccatttaatc agcagtattt ttgtgagcat caatacatgg ccatcggcac agtaaagtgg
     8641 ttcaacgacc aaaaaggttt cggtttcatc agccctgagg gcggcggcga agatctgttc
     8701 gcgcatttct ccgccatcac cggcggcggc ttcaagacgc tgcaggagaa tcagcgcgtc
     8761 gaatacgatg cgacccgcgg ccccaaaggc atgcaggcat cgaacattcg cccgctctga
     8821 gctgacgaac gttcaagaaa aaggccgcgc aagcggcctt ttttattgcc ctgcttcctc
     8881 cgctttcccg ctcgtcctga gcctgtcgaa ggacgaacgg actgactact tgcgcccggt
     8941 gcgctcgatg gactccagcg ccacgccgag gatgagtccg atgacgatac cgacggcgat
     9001 gttgccgtac atggcgccga tggccacgcc acaggcaatg ccgatcggca gccagggcga
     9061 catgcgccgg ttcagaaggt gatgtgtacg gaggaggcgc tgcgtttcgc ctcgccgtga
     9121 tagaccgctt cgatgttgtt gccgtccggg tcgagcacga aggcggcgta gtagcccggg
     9181 tgataaggcc gctcgccggg ctcgccgttg tccttgccgc cggcggcgag cgcggctttg
     9241 tggaaggcgt ccaccgcggc acggtcccgc gcctggaagg cgatgtgatt gcgtccggtg
     9301 agcatgccca gtgcggcggg actgtccgcc gccgagacga cgaactcgtc cgcccagaag
     9361 aaaccctcgc cttcgccctg cacgggaatg tcgagcacac tcaacaccgc gttatagaac
     9421 ttccgcgccg cagtcagatc cttcaccacc agttggatgt ggtcgatgag tcgcccgcga
     9481 tgcagttcgt tgacttccat acgtgtccgc tcccggcaat ggattgcgat ttgcacaggg
     9541 ccgcaatttc cgcgcccgct ccgtagatgt cgtctccggc gattcgattc gactttccgg
     9601 cttgcagcgt tgttaccatg cacgctttgc atggaggaca tcgccatgcc cggcaccatt
     9661 cgtttgcatc gcgtgctgca aaccacgccc gacaaacttt atcgcgcctt c
//
LOCUS       DATGOU010000076        20958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_5085879, whole genome shotgun sequence.
ACCESSION   DATGOU010000076 DATGOU010000000
VERSION     DATGOU010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 20958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20958
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5085879"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VKZ48_04500"
     CDS             complement(<1..356)
                     /locus_tag="VKZ48_04500"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013522039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HLU77151.1"
                     /translation="MSRVPNILAIAGVDPSGGAGILADVKAISANGGYACAVLAALTA
                     QNTRGVTGIEPVRTDFIRLQLDTLFEDVRIDAVKIGMLGAAPAIEVVADRLMAWKPPR
                     IVLDPVMVARSGDVLL"
     gene            complement(383..1741)
                     /gene="radA"
                     /locus_tag="VKZ48_04505"
     CDS             complement(383..1741)
                     /gene="radA"
                     /locus_tag="VKZ48_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HLU77152.1"
                     /translation="MAKPKTAYVCTECGGQAPKWQGQCPHCSGWNTLVESVAERAGGH
                     RYSGLAKASALTPLSDIRAAQAPRMQTGVTEFDRVLGGGLVQGQVALIGGDPGIGKST
                     LLLQALAAMSKQRKVIYVSGEESGEQVALRASRLQLETTNVGLLAEIQLEKISATLSA
                     EQPDVAVVDSIQTLYSEALQSAPGSVAQVRECAAQLTRLAKQSGITLIFVGHVTKEGA
                     LAGPRVLEHIVDTVLYFEGDTQSSFRLVRAFKNRFGPVNELGVFAMTEKGLRGVSNPS
                     ALFLSEHHAGVPGSCVLVTNEGTRPLLVEVQALVDTAHGMNPRRLAQGLDQNRLAMLL
                     AVLHRHAGVACHDQDVFINAVGGVRISEPASDLAVVLAVVSSFRNRALPEKLVVFGEI
                     GLAGEVRPAPRGLERLREAAKLGFTRALVPRANLPKQAIEGIEAVAIERLGDALEQLN
                     RL"
     gene            complement(1959..3029)
                     /gene="alr"
                     /locus_tag="VKZ48_04510"
     CDS             complement(1959..3029)
                     /gene="alr"
                     /locus_tag="VKZ48_04510"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HLU77153.1"
                     /translation="MPRPIRATFDLDALRANLALVRARAPNSRVWAVIKANAYGHGLL
                     RVAEALGQADGYALLDLDDAVRLREAGFRHPILLLEGCFELGDLYVAAEYSLTIVAHC
                     AAQIEMLEMSGLPVRMPVHLKLNTGMNRLGFTVSEFADAYERLKRCPAVGPITLMSHF
                     AEAEADNGVAEPLQVFEAAASGIEAPRTLANSAAILRFPQTCADWVRPGIMLYGCSPL
                     PDRSAEELGLRPVMTLTSEIIAIQNLRRGESTGYGRLFKAKSATRIGVVACGYADGYP
                     RHAPTGTPVLVNGQRTRTVGRVSMDMLAVDLTGVADADLGSPVTLWGEGLSADEVATA
                     AGTVSYELLCALAARVPQVELG"
     gene            complement(3071..3973)
                     /locus_tag="VKZ48_04515"
     CDS             complement(3071..3973)
                     /locus_tag="VKZ48_04515"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HLU77154.1"
                     /translation="MADRRLQVFHAVAKQMSFTKAAEVLFMTQPAVTFQIKQLEEHFN
                     TRLFERGHGKISLTPAGEVVLEYAERILSLSAELETRLREMTGRLSGPLLIGASMTIA
                     EFLLPRVLGQFKSAHPEVQAKLTVANSESIESRVAEHTLDIGLIEATSQHNSLVTEVC
                     CEDELQVIVAPEHPLAKAKSLTPQQLKEHPFIVRETGSGTRQYTDLYFKNAGLSNNDL
                     NIVMELGSSESVKGVVSTGLGYSILSRVTMAKELKLGELVAIPLQPRLQRSLSMIYPK
                     ERFRSRLINTFIEFTKTQLKNYVS"
     gene            4221..5534
                     /gene="lplT"
                     /locus_tag="VKZ48_04520"
     CDS             4221..5534
                     /gene="lplT"
                     /locus_tag="VKZ48_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008119424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HLU77155.1"
                     /translation="MKRGFYTIMAAQFFSSLADNALLIAAIQLLIELSSPAWMAPLLK
                     FFFTISYVVLSPFVGAFADSMPKGRVMFITNLVKILGCAMLFFYEHLTAPGWSPYVTV
                     LLAYGVVGLGAAAYSPAKYGILTELLPARQLVAANGWIEGTTVGSIILGTLLGGALVS
                     QTISEPLLAFDFPFIDTHVDTAAEAAILFIAMSYLIAAVINLYIPDTGAEHHVPHKNP
                     VYLVRDFAHCFHLLWRDKLGQISLATTTLFWGAGATLQFIVLEWARVALDYPLSQATM
                     LQGVVAVGIAVGAMAAAKIIPLKRSVSVLPVGVAMGLIVVSMIFVRDVWFAIPLMIAV
                     GGLAGFFVVPMNALLQHRGHILMGAGHSIAVQNFNENLSILVMLGVYAAMISLELSIH
                     TVIVIFGLFVSGTMVMVRLRHLYNQRQGDSLHLIGMEKPSHAPRS"
     gene            complement(5541..6041)
                     /locus_tag="VKZ48_04525"
     CDS             complement(5541..6041)
                     /locus_tag="VKZ48_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HLU77156.1"
                     /translation="MTSLKRLLRHLFAGDRAVKRAFPEACMRTIEAHIAAQERRHDGE
                     LLFVVENSLPLRELLAGLTAHERALELFSELCVWDTEQNAGVLIYLLLADRRVEIVAD
                     RGIHRKVGTAAWETICGAMQREFAAGRFEQGVLTGVQAISDLLAQHYPPQATNVDELA
                     NRPIVL"
     gene            complement(6044..6916)
                     /locus_tag="VKZ48_04530"
     CDS             complement(6044..6916)
                     /locus_tag="VKZ48_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009908362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HLU77157.1"
                     /translation="MLTLGRYLLVLLLFCAGLAQAQEQAVPTLESPVTDLTGTLTPDQ
                     RASLERLLLDFEKRKGSQIAMLIVPTTQPETIDQYSIRVVDHNKIGREKFDDGVLILL
                     AMQDRTARIEVGRGLEGAIPDVIADRIRRNVMNPRFQQGDFHGGLREAALLLMKAIEG
                     EQLPEAVRSAPRGQDFESLFVVLLMFTLIGGGALRAMFGKVFGSLATGGIAGVVAWLI
                     AGSLAAGIAAAILAFVFALTMAGTHGGVVRRGGHYWGGGRPGGGWGGGGGFGGGGWGG
                     GGGGFGGGGASGRW"
     gene            complement(6941..7552)
                     /locus_tag="VKZ48_04535"
     CDS             complement(6941..7552)
                     /locus_tag="VKZ48_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011850957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HLU77158.1"
                     /translation="MLSCVLLLSGCGYNQFQDQDEAVKAAWSEVLNQYQRRADLIPNL
                     VNTVKGYAQHEEQVLTEITEARARVGSVQATPELVDDPEAFARFQAAQSNLTSALSRL
                     LVVVENYPQLKADQNFRDLQAQLEGTENRIAVARNRYISAVQAYNSLTRRFPTNLTAM
                     VFGYKVKPNFTVENEREIAKPPAVDFGTRTSPATQPGAPAPAQ"
     gene            complement(7625..8197)
                     /locus_tag="VKZ48_04540"
                     /pseudo
     CDS             complement(7625..8197)
                     /locus_tag="VKZ48_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
     gene            complement(8356..8820)
                     /gene="rimI"
                     /locus_tag="VKZ48_04545"
     CDS             complement(8356..8820)
                     /gene="rimI"
                     /locus_tag="VKZ48_04545"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HLU77159.1"
                     /translation="MSAVLAPRPRFEIMRPADLDEVVTIERSVYEFPWTPGNFRDSIR
                     AGYDCRVYRSEHGTLIGYGVLMAGVDEAHLLNISVAAACQRHGYGSQLLEHFIDLTRA
                     RRLESIYLEVRPSNHVARRLYQRRGFRQVGLRRGYYPAVDGREDALVLKLAV"
     gene            complement(8817..9488)
                     /gene="tsaB"
                     /locus_tag="VKZ48_04550"
     CDS             complement(8817..9488)
                     /gene="tsaB"
                     /locus_tag="VKZ48_04550"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HLU77160.1"
                     /translation="MNLLALETSTDRLSLALLVDGRLHAFDQAVGQRHAEMILEEVGA
                     LMRQAGIAPRDLQGVVYGEGPGAFTGLRIACGVAQGLALATGAKVLGVGTLAAIAEES
                     GASRVVACIDARMSEVYHAVYEKQQGAWVTVQPPGLYAPDAVPLPPAGEWTGCGNGFA
                     AYAEALRVRLGARLVEVRSDVAPTASAMLRLAAPRFAAGEGGPAHTAIPTYLRDKVAL
                     KTSER"
     gene            9583..10332
                     /locus_tag="VKZ48_04555"
     CDS             9583..10332
                     /locus_tag="VKZ48_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLU77161.1"
                     /translation="MEFITDPWFYAVAIPTMLLVGISKGGLGLAGGLSVPLLSLVISP
                     FQAAAIMLPILIVMDLSAFLAYRRHWDRAQMRVLLPAGIVGIVVGAFVFSLLNEDWIR
                     VLIGVIAVGFVLHSLRKAVRVRPATNAKGFFWGGVAGFTSFVAHAGAPPLSVYLLPQR
                     LDPAVLVGTSVMFFTVINLGKLVPYAALGLLTVHNLSTSLLLAPLGALGIVLGIWLRT
                     RISTQLFYRLSYGLLLLTGSKLIHDGVGNLL"
     gene            10374..10997
                     /locus_tag="VKZ48_04560"
     CDS             10374..10997
                     /locus_tag="VKZ48_04560"
                     /inference="COORDINATES: protein motif:HMM:NF018693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid:lipid A palmitoyltransferase"
                     /protein_id="HLU77162.1"
                     /translation="MDARTQLATLLVFTLMAVNAPAAADWVEKVKQTTPFGMADEAEQ
                     REGLWRGLRAGSERLYKNGRWEVLLSGYAWHMPFEYSAEERDDYNERAWGLGFTRRVL
                     DERDNERLLFALVTKDSHSKPQYMAGYAWLARWRLSDSLRFGAGYAAFLMGRSAYGPG
                     NYIPFPAVLPLISIGTDRISLMATYVPGISRDRADGNVLFIFARLGF"
     gene            complement(11001..11618)
                     /locus_tag="VKZ48_04565"
     CDS             complement(11001..11618)
                     /locus_tag="VKZ48_04565"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HLU77163.1"
                     /translation="MTAASLSEARRRLRNGPTIVVLAGSNGAGKTTFHDHFLADLGWP
                     FVNADLIARELDPEQPERVAYEAAALADRMRRALVARRESFVMETVFSDPVGDKIAFL
                     REAQELGYAIFLMFIGLSDVQLSIARVVYRTEGGGHDVPDEKLLERYPRTLENLRQAI
                     PFVDLAIVLDNSAADDPYRHVATYERGKRVAKARTLPAWARELLP"
     gene            complement(11615..12097)
                     /locus_tag="VKZ48_04570"
     CDS             complement(11615..12097)
                     /locus_tag="VKZ48_04570"
                     /inference="COORDINATES: protein motif:HMM:NF023331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77164.1"
                     /translation="MGMPVKLSDELVLAARMEAQATDRTITSQIEHWAKLGRAAEAAL
                     PYGEVVKLKLAAFDPEVPVAKAKTSRSEMRKRLEGVAHARARDHVIEALRAAGRPVYE
                     ATPDHPGYVTRIEPDGKRMVGRFEGRRFVHQHPPGKSQEIACGCALSSPAKTKKVASR
                     "
     gene            complement(12156..12452)
                     /locus_tag="VKZ48_04575"
     CDS             complement(12156..12452)
                     /locus_tag="VKZ48_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77165.1"
                     /translation="MMKRLLVLVGMLCTLVAAYPAAAERNDYRHDPRYERNERRDNGR
                     SDWRDGRNSREQAGERRDRGGRMSPDERRQLRRDIQDHGRDVYRERGRRGRNGG"
     gene            complement(12524..13021)
                     /locus_tag="VKZ48_04580"
     CDS             complement(12524..13021)
                     /locus_tag="VKZ48_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77166.1"
                     /translation="MHTARTLLIALAALAVSGCATTYYDGPPAPYYYEPYPRFYGGVF
                     IHHHHSHVRPRFHRPPHFHSPSRVHPPHAKTPSRSHPRPPHAHKPDRHDKPDGRADRG
                     KRWNDGKGGDHGQRWNDGKRAGDGKGWGEGRRVERRESRGLDRGRSDRGRSGGPGHRR
                     GDGRR"
     gene            13212..13931
                     /gene="ompR"
                     /locus_tag="VKZ48_04585"
     CDS             13212..13931
                     /gene="ompR"
                     /locus_tag="VKZ48_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HLU77167.1"
                     /translation="MAEPKTKILVIDDDLRLRDLLNRYLAQQGFVVKTVSDATAMDKQ
                     LAQEHYDLMVLDLMLPGEDGLSICRRLRGTKNMIPIVMLTARGEDVDRIIGLEMGADD
                     YLPKPFNPRELVARINAVLRRRGAPLPPGAPATEPEVVEFGGMKLDLATRMLHKIDET
                     ISLTTGEFALLKVLVTHPRTPLSRDKLMELARRREYEVFDRAIDVQVSRLRKLIEPDP
                     SSPTYIQTVRGFGYVFVPDGQ"
     gene            13928..15226
                     /locus_tag="VKZ48_04590"
     CDS             13928..15226
                     /locus_tag="VKZ48_04590"
                     /inference="COORDINATES: protein motif:HMM:NF027838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLU77168.1"
                     /translation="MMPRSLLWRAFLLIAVVIVLAIGAWVQIFRAYDVEPRTRQVAQM
                     VVSVVNLTRTALVTAQPELRRELLVELVEREGIRIYPAEATDEIEELPHDRPQLQLLA
                     NEIRRDLGPQTRIARILNGERGFWVSFYIGEDEYWVMLPRERVERQLALQWVGWGALV
                     LVLALIAAYFIVFRLREPVAALTRAARDIGHGRTPPPLDDEDGPEELRTVSRAFNQMS
                     RNLAQLEEDRALILAGVSHDLRTPLARLRLGLEMSGVDEESLQAMSADIEEMDRIIGQ
                     FLDFARAAGGEEDAATDVGALADDLARHYRHTGHTLDAQIDDTGELAVKPMAIRRAIS
                     NLLDNAFRYGNGDVALRVRTTDREVSIEVLDRGPGIPIEDAERMKQPFTRLETARSGK
                     GGSGLGLAIVDRIARMHGGRFELLQREGGGLIAQVILPRA"
     gene            complement(15252..15773)
                     /gene="thpR"
                     /locus_tag="VKZ48_04595"
     CDS             complement(15252..15773)
                     /gene="thpR"
                     /locus_tag="VKZ48_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HLU77169.1"
                     /translation="MTATHARLFFALWPDAQTRAALVRWQRAVEPLCGGRVMHPDDVH
                     LTLAFLGDTPIGLLDPIKDLGSAVRGASFALRVDRLHYWKHNRIVWAGLEETPAALTA
                     LVDDLRARLIAADIRFDAKAFVPHITLFRNAQPVEVWPELPAIDWQVDGFALIRATGG
                     KGSRYRVEARWPL"
     gene            complement(15770..16249)
                     /gene="ispF"
                     /locus_tag="VKZ48_04600"
     CDS             complement(15770..16249)
                     /gene="ispF"
                     /locus_tag="VKZ48_04600"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HLU77170.1"
                     /translation="MNMRIGQGFDVHALVSGRPLIIGGVTIPYDRGLAGHSDADVLLH
                     AVTDALFGAAGLGDIGRHFPDTDEKYRGADSRVLLREAAARVRAAGYRVCNIDATVIA
                     QAPKMAPHIEAMARNIAEDVGIDAACVNIKAKTTERLGFTGRGEGIAAEAVTLIEAV"
     gene            complement(16246..16935)
                     /gene="ispD"
                     /locus_tag="VKZ48_04605"
     CDS             complement(16246..16935)
                     /gene="ispD"
                     /locus_tag="VKZ48_04605"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HLU77171.1"
                     /translation="MTHRLYGLIPAAGTGARLGGETPKQYLPLAGEAIIVHAVRALLT
                     HPDIDTVFVVLTPGDSRFGEIGWGEHADRVAPLYCGGPTRRDSVLNGLRAVRNLVEPD
                     DWMLVHDAARPGLTAEDLARLIDGVRDDDVGGILAVPLADTLKRADGGQRIVATAPRE
                     NLWRAQTPQMFRYGTLLRALDNAPEATDESGAVEQLGMQPKLILGSARNMKITYPGDM
                     ATAERLLQGRS"
     gene            17136..20582
                     /gene="mfd"
                     /locus_tag="VKZ48_04610"
     CDS             17136..20582
                     /gene="mfd"
                     /locus_tag="VKZ48_04610"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HLU77172.1"
                     /translation="MSYVVQTLAPPSAAVARRLRFGKLYGSSDALALAGAAQEARPLI
                     VVSASAVDARRLREEIAWFAPALRVSLLPDWETLPYDNFSPHQNLVSERLETLYHLST
                     GNCDVVIVPAATALHRLSPPSYLAAYTFFLKEGTRLDLTSFRQQMTVAGYAHVTQVVA
                     PGEYCVRGGLIDLFPMGSALPYRIDLVDDEIDTIRTFDVDTQRTIYKVNDVRLLPARE
                     FPMDDAARTRFRARFRETFEGDPTKSAVYKDVSNGIAPAGIEYYLPLFFEQTGTLFDY
                     LPPDATVALHHDVHGAIEAFWRDAQSRHNLLKGDRTRPIFAPGELFLSAENFFVALKD
                     FKRIDVQEAALGRDDSDAMQTLPLPDVAVERRAADPLHKLKAFIARAPGRLLVLADSP
                     GRRETMSEYFAEYGLPVALCGNYAEFTQASAPVMLAAAPIAGGFVFPAEQYAIVTESE
                     LYAGTARTRTRREEQRSNVENMLRDLSELSIGDPVVHETHGIGRYRGLVSMDVGEGDT
                     EFLSIEYLDQNKLYVPVAQLHVISRYAGGPPESAPLHRLGSDQWEKAKRKAAAQVRDT
                     AAELLNLYAQRATREGRRFEVRQHDLEAFAEGFGFEETPDQEAAISAVIEDLRSGKPM
                     DRLVCGDVGFGKTEVALRAAFVAAADGHQVAVLAPTTLLAEQHYQTFSDRFADFGLRI
                     AELSRFRTAKEAGAAIKGLADGSIDIVIGTHKLLSKDVKFKRLGLVIVDEEHRFGVRQ
                     KEVLKALRAEVDVLTLTATPIPRTLAMSLEGLRDLSVIATAPAKRLAIKTFVTPYSPS
                     VIRDAVMRELKRGGQVYFLHNDVATIESQREKLEALLPEARIVIAHGQMPERELERVM
                     RDFVQQRYNVLLCSTIIETGIDIPNANTILINRADKFGLAQLHQLRGRVGRSHHQAYA
                     YLLTPRFDALTSAAKKRLEAIQMMEELGSGFYLAMHDLEIRGAGEVLGESQSGDMLEV
                     GFNLYNDMLKAAVKALREGKEPDLSQPLGVTTEINLHMPALLPNDYCSDVNVRLSLYK
                     RLANCEDEDELAVMREELVDRFGPLPDAAQALLESHRLRILSRSLGIVRLDATAEQVQ
                     LHFGDKPSVDPARIIELIQKNRHWKLNGPDRLRVDIVSVALKDRVQMIRKVLEMLKGK
                     TQ"
     gene            20579..>20958
                     /locus_tag="VKZ48_04615"
     CDS             20579..>20958
                     /locus_tag="VKZ48_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013435129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4072 domain-containing protein"
                     /protein_id="HLU77173.1"
                     /translation="MNEATHNLIIQGPDAQESIAQSLSMLTGARAVQPLGPHAFRLRH
                     AHQRQAVLDVCRGWRIDHAYVPTTTRLDDHKLLAIDMDSTLITVECIDELADFAGVKA
                     QVSEVTQAAMRGEIDFGESLKRRVA"
BASE COUNT         3578 a         7087 c         6659 g         3634 t
ORIGIN      
        1 tcgagcagca cgtcgccgct gcgcgcgacc atcaccgggt cgagcacgat acgcggcggt
       61 ttccatgcca tgaggcggtc ggcgacgacc tcgatcgccg gcgcggcgcc gagcatgccg
      121 atcttcaccg catcgatgcg cacgtcctcg aacagcgtat cgagctgcag gcgaatgaaa
      181 tccgtgcgca ccggctcgat gccggtcacg ccgcgcgtgt tctgcgcggt aagcgcggcc
      241 agcactgcgc acgcgtaacc gccgttggcg ctgatcgcct tcacgtcggc cagaatcccg
      301 gcgccgccgg acggatcgac accggcgatg gcgagaatat tgggaacgcg actcatcgta
      361 aaaaccgggg actaaagcgc cactataggc gattgagctg ctccagcgca tcgcccaggc
      421 gctcgatcgc aaccgcttcg atgccctcga tcgcctgctt gggcagattg gcacgcggca
      481 ccagtgcgcg ggtaaagccg agcttggccg cttcgcgcag ccgctccaag ccgcgcggcg
      541 cgggtcgcac ttcgccggcc agaccgatct cgccgaacac caccagcttt tccggcagcg
      601 cgcgattgcg gaacgatgac accaccgcca gcaccactgc aagatcggac gcgggctcgc
      661 tgatacgcac cccgccgacg gcgttgatga acacgtcctg atcgtgacag gcgacgcccg
      721 cgtgacgatg cagcacggcc agcagcatgg ccagccggtt ctgatccagt ccctgcgcca
      781 gccgtcgcgg gttcatgcca tgcgccgtat cgaccagcgc ctgcacttcc accagcagcg
      841 gccgcgtgcc ttcgttcgtc accagaacgc agctgccggg cacgccggca tgatgttccg
      901 acagaaacag cgcggaagga ttcgacacac cgcgcagccc cttctccgtc atggcgaaca
      961 cgcccagttc gttcaccgga ccgaagcggt tcttgaacgc gcgcaccaga cggaaactcg
     1021 attgcgtatc gccttcgaaa tagagcacgg tatcgacgat gtgttcgagc acgcgcggtc
     1081 cggccagcgc gccttccttg gtgacgtgac cgacgaaaat cagcgtgatg ccgctctgct
     1141 tggccaggcg cgtcagttgc gcggcgcatt cgcgaacctg cgccaccgag ccgggcgccg
     1201 actgcaacgc ttcggaataa agcgtctgga tcgaatccac cacggcgaca tcgggctgct
     1261 cggccgacag cgtggcggag attttctcca actggatttc cgccagcagg ccgacgttgg
     1321 tcgtttccag ttgcagacgc gaggcgcgca gcgccacctg ctcgcccgac tcctcgccgc
     1381 tgacgtagat caccttgcgc tgcttcgaca tggcggcgag cgcctgcagc agcagcgtgg
     1441 atttgccgat acccggatcg ccgccgatca acgcgacctg cccctgcacc agaccgccgc
     1501 ccagcacccg gtcgaactcg gtgacgccgg tctgcatgcg cggcgcctgc gcggcgcgga
     1561 tgtccgacaa gggtgtcagc gccgaagcct tggcaagtcc gctgtagcga tgaccgcctg
     1621 cccgctccgc aacgctttcc accagcgtgt tccagccgct gcaatgcgga cactgccctt
     1681 gccatttggg cgcctgaccg ccgcattcgg tgcagacgta agcggtttta ggtttcgcca
     1741 ttagcgtcag attcacttct ttaaccacca agacaccaag gcgccaagga accccggtta
     1801 taacttggtg ttagctgtct ttcacagttc aacagttctc agccgacaac ttcgactcgc
     1861 acgacgcggt gacgcagagc ccggctctgg gtcactgtgg tccggttttt cgccttcctt
     1921 ggtgtcttgg tgtcttggtg ccttggtggt caaaatcttc agccgagctc gacctgcggc
     1981 acgcgcgccg caagcgcaca aagcaactcg tagctcaccg tgccggcggc ggtggcgacc
     2041 tcgtccgccg acaggccttc gccccatagg gtgacgggcg aaccgaggtc cgcgtcggca
     2101 acgccggtga ggtccaccgc aagcatatcc atcgagaccc ggcccaccgt gcgggtgcgt
     2161 tgtccgttga ccagtacggg cgtcccggtc ggcgcgtgcc gcggataacc gtcggcgtag
     2221 ccgcaggcca ccacgccgat gcgcgtggcc gacttggctt tgaacaggcg gccgtagccg
     2281 gtcgactccc cgcgccgcag attctggatg gcgatgattt cgcttgtgag cgtcatgacc
     2341 ggccgcagcc ccagttcctc ggcgctgcga tcgggcagcg gcgaacagcc gtacagcatg
     2401 atgcccggcc gcacccagtc ggcacaggtc tgcggaaaac gcagaatggc ggcggagttg
     2461 gccagcgtgc gcggcgcctc gatgccgctt gccgcggctt cgaacacctg cagcggttcg
     2521 gcaacgccgt tgtccgcctc cgcctcggcg aaatgcgaca tgagcgtgat cggtcccacg
     2581 gccgggcaac gcttgagccg ctcataggcg tcggcgaact cgctcaccgt aaacccgagc
     2641 cggttcatgc cggtgttgag cttcaggtgg acgggcatcc gcaccggcag tccggacatc
     2701 tccagcattt cgatctgcgc ggcgcaatgc gccacgatcg tgagcgaata ttccgcggcc
     2761 acgtacagat cgcccaactc gaagcagcct tccagcagca ggatgggatg gcgaaagccc
     2821 gcttcgcgca gccgtacggc atcgtccaga tcgagcagcg catatccgtc ggcctgcccc
     2881 agcgcctcgg ccacccgcag caggccgtga ccgtaggcat tggccttgat caccgcccat
     2941 acgcgggagt tgggcgcgcg ggcgcgcacc aacgcgaggt tggcgcgcag cgcatcgaga
     3001 tcgaaagtgg cgcggatcgg tctgggcatg gcaagaatgg tgaaacgcgg gcgcaggcgc
     3061 gcccgccgac tcaactcacg tagttcttca attgagtctt ggtgaattcg atgaaggtgt
     3121 tgatcaaccg cgagcggaaa cgctccttcg gatagatcat cgacagggac cgctgcagac
     3181 gcggctgcag cggaatggcg accagttcgc cgagtttcag ttccttcgcc atggtcacgc
     3241 gcgaaagaat cgaataaccc agtcccgtac tcacgacgcc tttcaccgat tcggaactgc
     3301 cgagttccat tacgatgttc aggtcgttgt tgctcaggcc ggcattcttg aaataaagat
     3361 cggtgtactg ccgcgtgccc gagccggtct cgcgcacgat gaacggatgc tccttcaact
     3421 gctgcggcgt caacgacttg gccttggcca gcggatgctc cggcgcaacg atgacctgca
     3481 attcatcctc gcagcagacc tcggtcacca gcgagttgtg ttgcgaagtc gcctcgatca
     3541 ggccgatgtc gagggtgtgc tcggcgaccc ggctctcgat ggattcggag ttggcgacgg
     3601 tcagtttcgc ctgcacctcg ggatgcgccg acttgaactg gccgaggaca cgcggcagca
     3661 gaaactccgc aatagtcatg gacgcgccga tcagcagcgg accggacagg cgtccggtca
     3721 tttcgcgcaa gcgcgtctcg agttcggctg acaaagacag tatcctttcg gcgtattcca
     3781 gcaccacctc gcctgccggc gtgagggaaa tcttgccgtg cccccgctcg aacagacggg
     3841 tgttgaagtg ctcttccaac tgcttgatct gaaaggtcac ggcaggctgc gtcatgaaca
     3901 gcacttccgc cgctttggtg aagctcatct gcttcgccac ggcgtgaaaa acctgcagtc
     3961 tacgatcggc catagtcgcg tccggagagc cagccgacac cctggccggc aacgaggctt
     4021 attcaaccat aaaacgcact taggacggaa cttatagggt ccggatccgg ccgaacccgt
     4081 cggccctgca ccggaaagcc ctgtgtcgtc aagccgataa gcgttcgaac gcagcaaacc
     4141 tgccgtggta taagagcggc ggcctcacga cccacccaga agagtcctca ccgaaggact
     4201 ccagtcagtg ttccactttg atgaaacgcg gtttctacac catcatggcg gcgcagtttt
     4261 tttcgtcgct cgccgacaat gcgctactga tcgctgccat ccagttgctc atcgaactgt
     4321 ccagtccggc ctggatggct ccactgctga agttcttctt caccatttcc tacgtcgtgc
     4381 tgtcgccgtt cgtcggcgcg ttcgcagact cgatgcccaa gggtcgggtc atgttcatca
     4441 ccaatctggt gaagatcctc ggctgtgcga tgctgttctt ctacgagcac ctcaccgcgc
     4501 ccggctggtc gccgtatgtg acggtgctgc tggcctacgg ggtggtcggt cttggcgccg
     4561 cggcctactc gccggccaag tacggcatcc tcaccgaact gctaccggcc aggcaactgg
     4621 tggcggcgaa cggctggatc gaaggcacga cggtcggctc catcatcctg ggcacgctgc
     4681 tgggcggcgc gctggtcagc cagaccattt ccgagccact gctggctttc gatttcccgt
     4741 tcatcgatac gcacgtggac actgcggcgg aagcggcgat cctgttcatc gccatgtcct
     4801 atctgatcgc ggcggtgatc aatctctaca ttccggacac gggggccgag caccacgtgc
     4861 cgcacaagaa ccccgtctat ctggtgcgcg acttcgccca ctgcttccac ctgctgtggc
     4921 gcgacaagct gggccagatc tcgctcgcca ccacgacact gttctggggt gcgggcgcga
     4981 cgctgcagtt catcgtgctg gagtgggcgc gcgtggcgct ggactatccc ttgtcccagg
     5041 cgacgatgct gcaaggcgtg gtcgccgtcg gcatcgcggt cggcgccatg gccgcggcca
     5101 agatcattcc gctcaagcga tcggtgagcg tgctgccggt gggcgtcgcc atgggattga
     5161 tcgtggtgtc gatgattttc gtccgcgacg tgtggttcgc catcccgctc atgatcgccg
     5221 tcggcggtct ggccggtttc ttcgtcgtgc cgatgaacgc cctgctgcag caccgcgggc
     5281 acattctcat gggcgcaggc cattcgattg cggtgcagaa cttcaacgag aacctgtcga
     5341 tcctggtcat gctcggcgtc tatgccgcca tgatcagcct ggagctgtcg attcatacgg
     5401 tgatcgtgat tttcggactg ttcgtcagcg gcacgatggt aatggtgcgc ctgcgccacc
     5461 tgtacaacca gcgccagggc gattcgctgc acctcatcgg catggagaag cccagccatg
     5521 ctccgcgctc gtaggtagcg ctacagcacg atcgggcgat tggccagctc gtcgacgttg
     5581 gtcgcctgcg gcggatagtg ctgtgcgagc aggtcgctga ttgcctggac accggtcagc
     5641 acgccctgtt cgaaccgtcc ggcggcgaat tcgcgctgca tggcaccgca gatggtctcc
     5701 cacgccgccg tacccacttt gcgatggatg ccgcggtcgg caacgatctc gacgcggcga
     5761 tcggcaagca gcagatagat cagcacgccg gcattctgct ccgtatccca gacgcacaat
     5821 tcgctgaaca gttccagcgc gcgctcatgc gccgtcagtc cggcaagcaa ctcacgcagc
     5881 ggcaggctgt tctcgaccac gaacagcagt tcgccgtcat gccgtcgctc ctgcgccgcg
     5941 atatgcgctt cgatcgtccg catgcaggcc tcggggaaag cgcgcttcac cgcccgatcg
     6001 ccggcaaaca gatggcgcag caaacgcttg agggacgtca tgctcaccac cgtcccgacg
     6061 caccgccgcc gccgaagccg cctccgccgc cgccccagcc gccgccgccg aaaccgccgc
     6121 caccacccca cccgccgccg ggacggccgc cgccccagta gtgcccgccg cgccgcacca
     6181 cgccgccatg cgttcccgcc atggtgagcg cgaagacgaa ggccagtatg gccgcggcga
     6241 tacccgcggc gagcgaaccg gcgatcagcc aggcgacgac gccggcgata ccgcccgtgg
     6301 cgagcgaacc gaacaccttg ccgaacatcg cgcgcaacgc gccaccgccg atgagcgtga
     6361 acatcagcag cacgacgaac agcgattcga agtcctgtcc gcgcggggcg ctgcgtaccg
     6421 cttcgggcaa ctgctcgcct tcgatggcct tcatcagcag gagcgcggcc tcgcgcaagc
     6481 cgccgtggaa atcgccttgc tgaaaccgcg ggttcatgac gttgcggcgg atgcggtccg
     6541 cgatcacatc gggaatcgcg ccctcaagtc cgcgcccgac ttcgatgcgg gccgtgcgat
     6601 cctgcatcgc cagcagaatg agcacgccgt cgtcgaactt ctcgcgcccg atcttgttgt
     6661 gatcgaccac gcgaatggag tactggtcga tcgtctccgg ctgggtcgtc ggcacgatca
     6721 gcatcgcgat ctggctgcct ttgcgcttct cgaagtcgag cagcagccgc tcgagcgagg
     6781 cgcgctgatc gggcgtcagc gtgccggtga gatcggtgac cggactctcg agcgtcggca
     6841 ccgcctgctc ctgcgcctgc gcaaggccgg cacagaacag gagcagaacg aggagatatc
     6901 gaccgagcgt cagcacgagg cgagcgggcg gtcgcgcgga ttattgcgcc ggcgccggtg
     6961 cgccgggctg ggtcgcgggg gaggtgcgcg taccgaaatc caccgccggc ggcttggcga
     7021 tttcgcgttc gttctccacc gtgaagttcg gcttcacctt gtagccgaac accatggcgg
     7081 tcaggttcgt ggggaagcgc cgcgtcaggc tgttgtacgc ctgcaccgcg ctgatatagc
     7141 gattgcgcgc caccgcgatg cggttttccg tgccctccag ttgcgcctgc agatcgcgga
     7201 aattctgatc ggccttcaac tgcggatagt tctccacgac gacgagcaga cgcgacagcg
     7261 ctgaggtgag attggactgc gccgcctgga agcgcgcgaa ggcttccgga tcgtcgacga
     7321 gctcgggtgt ggcctgcacg ctgcccacac gggcgcgcgc ctcggtgatc tcggtcagca
     7381 cctgttcctc gtgctgcgca taacccttca ccgtgttgac caggttggga atgaggtcgg
     7441 cgcgacgctg gtactgattc agcacttcgg accacgcggc cttcaccgcc tcgtcctgat
     7501 cctgaaactg gttgtagccg cagccggaaa gcagcagcac gcatgacagc atgaacgcgg
     7561 tccagacttt cgacacgatc gacctcctcg ttttggaatt attcgtcgcg cgccgccggc
     7621 aggctcacgc gctttgcagc gcctgcatgg ttgcgcgcgc gaagcacaag tcctcccatg
     7681 ccttggcctt gtcaggcagg ttccgtagca gataagccgg atggtaggtc acgatcagcg
     7741 gcacgccatg atagtcgtgc cgcttcccgc gcaggctcgc aatgctggcg tccgtgttaa
     7801 gcaaggtttg cgccgcgaaa cgacccatgg cgatcaacag ccgcgggcgc accagttcga
     7861 tctggcgctt gagatagggc aggcattgct cgatctcctc cggtgcgggg ttgcggttgc
     7921 ccggcggccg gcatttgagg acattggcga tataaacgtg ttcgcgccgg ttgagatcga
     7981 tggcggcgag catctgatcg agcagcaggc cggcctggcc gacaaacggc tcgccgcggg
     8041 catcctcttc ggcgcccggc gcctcgccga caatcatcca gtcggcttcg ctcggaccca
     8101 cgcccggcac cgcctgcttg cgcgagcgcc ccagcgcgca cgcctggcaa cccgtgatgg
     8161 ctgcgcgtag cgcatcccat tgcatttccg ccacggggtg cgcggaaggc ggtgcgacac
     8221 gctcgaccgt ttcgatcccg gcggcttccg ccacggcgct ttccgctgcg ccgccttcgt
     8281 cgggcgtagc cgtcccgcgc agacgccaca cgggagtcag ccccatctcc tccagcaggg
     8341 ccaggcgacg cggcgtcata cggcgagctt cagcaccagg gcgtcctcgc gcccatcgac
     8401 tgccgggtaa tagccgcgac ggagtcccac ctgacggaat ccgcgccgct gataaagacg
     8461 ccgtgcgaca tgattggacg gccgcacttc cagatagatc gattccagcc gccgcgcacg
     8521 cgtcagatcg atgaaatgct ccagcagttg tgaaccgtag ccgtggcgct ggcaggcggc
     8581 ggccacactg atattcagca gatgcgcttc gtccacgccc gccatcagca cgccgtagcc
     8641 gatcagggtg ccgtgttcgc tgcgatagac gcggcagtcg tagcccgcgc ggatggagtc
     8701 gcggaagttg cccggcgtcc agggaaattc gtagaccgat cgctcgatgg tcacgacttc
     8761 gtcgagatcg gcgggccgca tgatttcgaa gcgcggccgg ggtgcaagaa cggcactcat
     8821 cgttcgctcg ttttcagggc gaccttgtcg cgcagataag tggggatcgc ggtatgagcc
     8881 ggcccgccct cgcctgccgc gaaacgcggc gcggccagac gcagcatggc gcttgcggtc
     8941 ggcgcgacat cggagcgcac ctcgaccaga cgcgcgccca ggcgaacccg cagcgcctcg
     9001 gcatacgcgg cgaaaccgtt gccgcagccg gtccattcgc ccgccggcgg cagcggcacc
     9061 gcatcggggg cataaagacc ggggggctgc accgtcaccc acgctccctg ctgtttttcg
     9121 tagacggcat ggtacacctc gctcatgcgc gcatcgatgc acgccaccac gcgcgacgcg
     9181 ccgctttcct ccgcgatcgc cgccagcgtg ccgacgccga gcactttcgc gccggtcgcc
     9241 agcgcaagtc cttgtgccac gccgcaggca atgcgcaagc cggtgaaagc gccggggcct
     9301 tcgccgtaaa cgacgccctg caggtcgcgc ggcgcaatgc ccgcctgccg catcagcgcg
     9361 ccgacttcct ccaggatcat ttcggcgtgg cgctgcccga ccgcctgatc gaaggcatgc
     9421 agccgtccgt cgacaagcag cgcgagcgac agacggtcgg tggaggtttc gagtgcgagc
     9481 aggttcatgg gatgtggcgc gtcgattgta acctgccggg acaagccgcc acgccggagc
     9541 gcgcgtggga taatcgcagc cttcccgccg tcgcccgcta tcttggaatt catcaccgac
     9601 ccctggtttt atgccgtcgc catcccgacc atgctgctgg tcggcatctc caagggcggc
     9661 ctgggtctgg ccggcggact gtcggtaccg ctcctcagct tggtgatctc gccgtttcag
     9721 gctgcggcca tcatgctgcc catcctgatc gtgatggatc tatccgcgtt cctcgcctac
     9781 cgccggcact gggaccgcgc gcagatgcgc gtcctcctcc ccgccggcat cgtcggcatc
     9841 gtcgtcggcg ccttcgtgtt cagcctgctg aacgaagact ggatacgcgt gctgatcggc
     9901 gtgatcgccg tgggtttcgt gctccatagt ctgcgcaaag ccgtgcgcgt gcggcccgcc
     9961 accaatgcga aaggcttttt ctggggcggc gtggccggct tcaccagctt cgtcgcccat
    10021 gcgggcgcac cgccgctttc ggtctatttg ctgccgcagc ggctcgatcc cgcggtgctg
    10081 gtcggcacct cggtgatgtt cttcaccgtg atcaacctgg gcaaactcgt gccctatgcc
    10141 gcgctggggc tgctaacggt gcacaacctg tccacttccc tgttgctcgc cccgctgggc
    10201 gcgctgggca tcgtgctggg catctggctg cgcacgcgca tcagcacgca actgttctac
    10261 cgcttgtcct acggactgtt gctgctgacc ggcagcaagt tgatccatga cggagtcggc
    10321 aatctgcttt gacgcatcgc ctgcgccgca tggcgcttgc atcgcatcgg tggatggacg
    10381 cccgaacgca actcgccacg ctgttggttt tcacgcttat ggccgtcaat gcgcctgcgg
    10441 cggcggactg ggtggagaag gtgaagcaaa ctacgccctt cggtatggcg gacgaagccg
    10501 agcagcgcga aggtttgtgg cggggactgc gcgccgggag cgaaagactc tacaaaaacg
    10561 gccgctggga agtgctgttg tcgggctatg cctggcacat gcccttcgaa tacagcgccg
    10621 aggaacggga cgattacaac gagcgcgcct ggggcctggg attcacgcgc cgcgtactgg
    10681 acgaacgcga caacgaacgc ctgctttttg cgctcgtcac taaagattcg cacagcaagc
    10741 cgcaatacat ggccggctac gcctggctgg cgcgctggcg tctgtcggac agtctgcgtt
    10801 tcggcgcggg ttacgccgcc tttctgatgg ggcgcagcgc atacggcccc ggcaactaca
    10861 ttcccttccc cgcggtgctg ccgctgatca gcatcggcac cgatcgcatt tcgctgatgg
    10921 cgacctacgt gccgggcatc tcgcgcgacc gtgccgacgg caacgtgctg ttcatcttcg
    10981 cccggctcgg cttctgaacg tcagggcaac agctcgcgcg cccacgccgg cagcgtgcgc
    11041 gctttggcaa cacgcttgcc gcgctcgtag gtggcgacat ggcgataggg atcgtcggca
    11101 gcgctgttgt cgagcacgat ggccagatcg acgaagggaa tggcctgacg caggttctcc
    11161 agcgtgcgcg gatagcgctc gagcagtttc tcgtccggca catcgtgtcc gccgccttcg
    11221 gtgcggtaca ccacgcgcgc gatcgacaac tgcacgtcgc tcaggccgat gaacatcagg
    11281 aagatcgcgt agcccagttc ctgcgcctcg cgcagaaagg cgatcttgtc gccgaccgga
    11341 tcggaaaaca ccgtttccat gacgaaggac tcgcgccgcg ccaccagcgc gcgacgcatg
    11401 cgatcggcca gcgccgcagc ttcgtaggcc acgcgctcgg gctgctcggg atcgagttcg
    11461 cgcgcgatca ggtcggcgtt gacgaacggc cagcccaggt cggcgaggaa gtgatcgtga
    11521 aaggtggtct tgccggcgcc gttggagccg gccagcacca cgatggtggg gccgttgcgc
    11581 aaacggcggc gagcctcgga caggctcgcg gccgtcatcg gctggcgact ttcttcgtct
    11641 tggcgggact gctcagcgcg cagccgcagg cgatctcctg cgacttgccg ggcggatgct
    11701 gatgcacgaa gcggcgtcct tcgaagcggc ccaccatgcg cttgccgtcg ggttcgatgc
    11761 gcgtcacgta gccggggtga tcgggcgtgg cctcgtacac cggccggccc gccgcgcgca
    11821 gcgcttcgat cacgtgatcg cgggcacggg cgtgcgccac gccttccagc cgcttgcgca
    11881 tttcgctgcg cgaagtcttg gctttggcga ccggcacttc gggatcgaag gcggcgagct
    11941 tcagcttgac gacctcgcca tagggcagcg cggcttccgc ggcgcggccc agtttggccc
    12001 aatgctcgat ctgggaagta atggtgcgat cggtggcttg cgcctccatg cgcgccgcga
    12061 gcacgagctc atcggaaagt ttgaccggca ttcccatcgc gtcctccgct tcggcggtgt
    12121 tgacggtagt aacttactac cgcagcgggc cggcgtcaac cgccgttgcg tccccggcgg
    12181 ccgcgttcgc gatagacgtc gcgcccgtga tcctgaatgt cgcggcgcag ttgccggcgc
    12241 tcgtcgggcg acatccgccc gccccggtcg cgccgctcgc ccgcctgttc gcggctgttg
    12301 cggccgtccc gccagtcgct gcgtccgttg tcgcggcgct cattgcgttc atagcgcgga
    12361 tcgtggcgat agtcattgcg ctcggcggcc gccggatagg cggcgacgag cgtacacagc
    12421 atgccgacca gcacgagcag gcgtttcatc atttgcggga ttgtccggaa tcgttgtcga
    12481 tgggcgatca ggcgtctgtc gcgcgccgca cattgtatgt aaatcagcgg cgcccgtcgc
    12541 cgcgacggtg accgggcccg ccgctgcgcc cccggtcgct gcgcccccgg tcgaggccgc
    12601 ggctttcgcg ccgctcgacg cgacggccct cgccccaccc tttgccgtcc ccggcacgct
    12661 tgccgtcgtt ccaccgttga ccgtggtcgc cacccttgcc gtcgttccac cgtttgccgc
    12721 ggtcggcgcg cccgtccggc ttgtcgtgcc ggtccggctt gtgcgcatgc ggcgggcgcg
    12781 gatgcgatcg cgacggcgtt ttcgcatgcg gcggatgcac gcgcgacggc gaatggaagt
    12841 gcggcggccg gtggaaacgc ggccggacat gactgtgatg gtgatggatg aatacgccgc
    12901 cgtagaaacg cggatagggt tcgtagtaat aaggtgcggg cggcccgtcg taataggtcg
    12961 tcgcgcaccc cgatacagcg agggcggcaa gggcgatcaa caatgtgcgc gcggtatgca
    13021 tggccggctc ctggaagtgg cctcaggcta cgtcccgcgt gtaagcccag tgtgtatcgc
    13081 cgcgctgttt tgtaagcgtt tgttgcagaa cccgggcggc ttacgcggtg taacgacttc
    13141 ccgcgtccgt cgcaccgtcc gtgcccgcct cgggctagga tatggttccc gagatgccaa
    13201 gctgtcgtga catggccgaa cccaaaacca aaatactcgt gatcgacgac gatctgcgcc
    13261 tgcgcgatct gctcaaccgc tacctcgccc agcagggctt cgtggtgaag acggtgtccg
    13321 acgccacggc catggacaaa caactggcgc aggagcatta cgatctcatg gtgctcgacc
    13381 tgatgctgcc gggcgaggac ggcctgtcca tctgccgccg tctgcgcggc acgaagaaca
    13441 tgattcccat cgtcatgctc accgcgcgcg gcgaggatgt ggatcgcatc atcggcctgg
    13501 aaatgggcgc ggacgactac ctgcccaaac ccttcaaccc gcgcgaactg gtcgcgcgca
    13561 tcaacgcggt ccttcgccgc cgcggcgcgc cgctgccgcc cggtgcgccg gccaccgagc
    13621 cggaggtggt cgagttcggc ggcatgaaac tcgatctggc cacgcgcatg ctgcacaaga
    13681 tcgacgagac gatctcgctc accaccggcg aattcgccct gctcaaggtg ctggtgacgc
    13741 atccgcgcac gccgctgtct cgcgacaagc tgatggaact ggcgcgccgg cgcgaatacg
    13801 aggtattcga tcgcgccatc gacgtgcagg tgtcccgtct gcgcaagctg atcgagcccg
    13861 atccgtccag tcccacctac atccagaccg tgcgcggttt cggctacgtc ttcgtgcccg
    13921 acggccaatg atgccgcgca gtctgctgtg gcgcgccttt ctgctcatcg ccgtggtgat
    13981 cgtgctcgcc atcggcgcct gggtgcagat cttccgcgcc tacgacgtcg agccgcgcac
    14041 gcgccaggtc gcgcagatgg tggtgagcgt ggtcaacctc acgcgcacgg cgcttgtcac
    14101 cgcgcagccg gaattgcgcc gcgaactgct cgtcgaattg gtggagcggg aaggcatccg
    14161 catctacccg gcggaggcga cggatgagat cgaggaactg ccgcacgacc gtccgcaact
    14221 gcaactgctc gccaacgaga ttcgccgcga cctcgggccg cagacacgca ttgcgcgcat
    14281 cctcaacggc gaacgcggtt tctgggtgag cttctatatc ggcgaggacg aatactgggt
    14341 gatgctgccg cgcgaacgcg tggagcgcca gctcgcgctg caatgggtgg gctggggcgc
    14401 actggtgctc gtcctcgcac tgatcgccgc ctacttcatc gtctttcggc tgcgcgagcc
    14461 ggttgccgcc ctcacccgtg ccgcgcgcga catcggccac ggccgcacgc cgccgccgct
    14521 ggacgatgaa gacggccccg aggaactgcg caccgtctcg cgcgccttca atcagatgtc
    14581 gcgtaatctg gcgcaactcg aagaagaccg cgccttgatc cttgccggcg tctcgcacga
    14641 tttgcgcacg ccgctcgcgc gtctgcgtct cggtcttgaa atgagcggcg tggacgagga
    14701 aagcctgcag gcgatgtcgg ccgacatcga ggagatggat cgcatcatcg gccagttcct
    14761 cgatttcgcg cgcgccgcgg gcggcgaaga ggatgccgcc accgatgtgg gcgccctcgc
    14821 cgacgatctc gcgcgccatt accgccacac cggccatacg ctggacgcgc aaatcgacga
    14881 caccggcgaa ctcgccgtca agccgatggc catccgccgc gcgatctcca acctgctcga
    14941 caacgccttc cgctacggca acggcgacgt cgcgctgcgc gtgcgcacga ccgacaggga
    15001 agtttccatc gaagtactgg atcgcggccc cggcattccc attgaagacg ccgaacgcat
    15061 gaagcagccc ttcacgcgac tggaaaccgc acgcagcggc aaaggcggct cgggcctggg
    15121 actggccatc gtcgatcgca tcgcgcgcat gcacggcggc cgcttcgaat tattgcaacg
    15181 cgaaggcggc ggattgattg cgcaagtgat tttgcctcga gcgtgagcgc catggacggc
    15241 cgctcgacgc gctaaagcgg ccaacgcgct tccacccggt aacgcgaccc tttgccgccc
    15301 gtcgcgcgga tgagcgcaaa gccgtccacc tgccagtcga tggcgggcaa ctcgggccac
    15361 acttcgaccg gctgcgcatt gcggaacagg gtgatgtgcg gcacgaaggc tttggcatcg
    15421 aagcggatgt ccgccgcaat cagccgtgcg cgcagatcgt cgaccagcgc cgtcaacgca
    15481 gccggcgtct cctccagccc cgcccagacg atgcggttgt gcttccagta gtgcagccga
    15541 tccactcgca gcgcgaagga cgcgccgcgc acggccgatc caaggtcctt gatcggatca
    15601 agcaacccga tcggcgtatc gcccaggaac gccagggtca gatgcacatc gtccggatgc
    15661 atgacgcgcc cgccgcacag cggttccacc gcacgctgcc accggacgag cgccgcgcgc
    15721 gtctgcgcat cgggccacag cgcgaagaac agtcgcgcgt gcgtcgcggt cacacggcct
    15781 cgatgagcgt caccgcttcg gcggcgatgc cttcgccgcg accggtgaag cccagtcgct
    15841 cggtggtctt ggccttgatg ttgacgcacg cggcatcgat gccgacgtcc tcggcgatgt
    15901 tgcgcgccat cgcttcgata tgcggcgcca tcttgggcgc ctgcgcgatg acggtcgcat
    15961 cgatattgca gacgcgatag cccgccgcgc gcacacgcgc cgccgcctcg cgcagcagga
    16021 cgcggctgtc ggcaccgcga tacttctcgt ccgtatcggg gaagtgtcga ccgatatcac
    16081 ccagccccgc cgcgccgaac agcgcgtcgg tgacggcgtg cagcagcaca tcggcatcgg
    16141 agtgtcccgc cagccccctg tcgtaaggaa tggtcacgcc gccgatgatc agcggtcgtc
    16201 cgctcaccag cgcatgcacg tcgaagccct gtccgatacg catgttcatg aacgcccctg
    16261 cagcagccgt tcggcggtgg ccatgtcgcc cggataggtg atcttcatat tgcgcgcgct
    16321 gccgagaatg agtttgggtt gcatgccgag ttgctccacg gcgccggatt cgtccgtggc
    16381 ctccggcgcg ttgtcgagcg cgcgcaacag cgtgccgtaa cggaacatct gcggcgtctg
    16441 cgcacgccag aggttttcgc gcggcgcggt ggcgacgatc cgctgcccgc catcggcgcg
    16501 cttgagcgta tcggccagcg gcacggcaag aatcccaccc acgtcgtcgt cgcgtacgcc
    16561 gtcgatcagc cgcgcgaggt cttccgcggt cagtcccggc cgcgccgcgt cgtggacgag
    16621 catccaatcg tcgggctcga ccagattgcg caccgcgcgc aggccgttga ggacgctgtc
    16681 gcgccgggtg ggcccaccgc aatagagcgg cgccacgcgg tcggcatgtt cgccccaccc
    16741 gatttcgccg aaccgggaat ccccgggcgt gaggacgacg aacaccgtgt cgatgtccgg
    16801 gtgcgtaagc agggcgcgca cggcgtgcac gatgatcgcc tcgccggcga ggggcagata
    16861 ttgcttgggc gtctcgcccc cgaggcgggc gccggtgccc gccgccggaa tgaggccgta
    16921 taaacgatgg gtcatgtgtc gggtctgtgc tcgatcattg gaggcgcatc gcacgggtga
    16981 accgcgggcg cgcacggcga agcccgtata atagcggcct cgcatgccgc ctcccaaagc
    17041 ggcattgcgt ttgcccccgc gtaaggctca agcccgcttg aactgcttac gcgggttttg
    17101 tcgtttctgc gtcgcccggg cggcgcgcaa tctgaatgtc atacgtagtg cagacactcg
    17161 ctccaccttc ggccgccgtc gccagacgtt tgcggttcgg caagttgtac ggttcctcgg
    17221 atgctttggc gctcgcaggc gccgcgcagg aggcgcggcc gctgatcgtc gtcagcgcct
    17281 cggccgtcga tgcgcgccga ttgcgcgagg aaatcgcctg gttcgcgccg gcgttgcggg
    17341 tgagccttct gccggactgg gagacgctgc cctacgacaa tttctcgccg catcagaatc
    17401 tggtgtcgga gcggctggaa acgctctacc acctcagcac cggcaactgc gatgtggtca
    17461 tcgtgccggc ggcgacggcg cttcatcggc tttcgccgcc gtcgtacctg gccgcctaca
    17521 ccttcttcct caaagagggc acgcgcctcg acctgacgag tttcaggcag cagatgacgg
    17581 tggccggcta cgcccatgtc acgcaggtcg tcgcccccgg cgaatattgc gtgcgcggcg
    17641 gcctgatcga tctcttccca atgggcagcg ccctgcccta tcgcatcgat ctggtggacg
    17701 acgagatcga caccatccgc accttcgacg tcgatacgca gcgcacgatc tacaaggtca
    17761 acgacgtgcg cctgctgccg gcgcgcgaat tcccgatgga cgatgcggcg cgcacgcgct
    17821 tccgcgcgcg ttttcgcgaa accttcgaag gcgatcccac caagagcgcg gtgtacaagg
    17881 acgtcagcaa cggcatcgca ccggcgggca tcgagtacta cctgccgctg ttcttcgagc
    17941 agaccggcac gctgttcgac tacctgccgc ccgatgcgac ggtggcgctg catcacgacg
    18001 tgcacggcgc catcgaggcg ttctggcgcg atgcgcaatc gcgccacaac ctgctgaagg
    18061 gcgatcgcac ccggccgatc ttcgcgcccg gcgaactgtt cctcagcgcg gagaactttt
    18121 tcgtcgcgct caaggatttc aagcgcatcg acgtgcagga agcggcgctc ggccgggacg
    18181 attccgacgc catgcaaacg ctgccgctgc ccgatgtggc ggtggagcgc cgcgccgccg
    18241 atccgctgca caaactgaag gctttcatcg cccgcgcgcc cggacggttg ctcgtgctgg
    18301 ccgattcgcc cggccggcgc gaaaccatga gcgagtattt cgccgagtac ggcctgccgg
    18361 tcgcactctg cggcaactac gccgaattca cccaggcaag cgcaccggtc atgctggccg
    18421 cggcgccgat cgccggcggc ttcgtcttcc ccgccgagca atacgccatc gtcaccgagt
    18481 ccgagcttta cgccggcacg gcgcgcaccc gcacacgacg cgaagaacag cgcagcaacg
    18541 tcgagaacat gctgcgcgac ctgtcggaac tgtcgatcgg cgatccggtg gtgcacgaga
    18601 cgcacggcat cggacgttat cgcggcctcg tcagcatgga cgtcggcgaa ggcgacacgg
    18661 aattcctgtc gatcgaatat ctcgaccaga acaagctgta cgtgccggtc gcgcaattgc
    18721 acgtcatcag ccgctatgcc ggcgggccgc ccgagtcggc gccgctgcac cggctgggca
    18781 gcgatcagtg ggagaaggcc aagcgcaagg ccgccgcaca ggtgcgcgac accgcggccg
    18841 aactgctcaa cctctatgcg cagcgcgcca cgcgcgaagg gcgtcggttc gaagtccgtc
    18901 agcacgatct cgaggccttc gccgaaggct tcggcttcga ggaaacgccc gatcaggaag
    18961 cggccatcag cgccgtcatc gaagacctgc gcagcggcaa gcccatggac cgtctggtgt
    19021 gcggcgacgt gggcttcggc aaaacggaag tcgcgttgcg cgccgcattc gtcgccgccg
    19081 cggacggcca ccaggtcgcg gtgctcgcac cgacgacgct gctggccgaa cagcattacc
    19141 agaccttcag cgaccgcttc gccgacttcg gcctgcgcat cgccgaactg tcgcgcttcc
    19201 gcacggccaa ggaagccggg gcggcgatca agggactggc cgacggcagt atcgatatcg
    19261 tcatcggcac gcacaagctg ttgtccaagg acgtgaagtt caaacggctc gggctggtga
    19321 tcgtggacga ggaacaccgc ttcggcgtgc ggcaaaagga agtgctcaag gcactccgcg
    19381 cggaagtcga tgtgctcacg ctcaccgcca cgcccattcc gcggacgctc gccatgtcgc
    19441 tggaaggcct gcgcgacctg tcggtcatcg ccactgcgcc ggccaagcgc ctggcgatca
    19501 agacgttcgt cacgccctac tcgcccagcg tgatccgcga cgcggtcatg cgcgaactca
    19561 agcgcggcgg tcaggtgtat ttcctccaca acgacgtcgc caccatcgaa tcgcagcgcg
    19621 agaaactgga ggcgctgctg cccgaggcgc gcatcgtcat cgctcacggc cagatgcccg
    19681 agcgcgaact ggagcgcgtg atgcgcgact tcgtgcagca acgctacaac gtgctgctgt
    19741 gctccaccat tatcgagacg ggcatcgaca tcccgaacgc caacaccatc ctgatcaacc
    19801 gcgccgacaa gttcggcctg gcgcagttgc atcagttgcg ggggcgcgtc gggcgctcgc
    19861 accatcaggc ctacgcctac ctgctgacgc cgcgcttcga tgcgctcacc tcggcggcga
    19921 agaagcgcct ggaagccatc cagatgatgg aagagctggg ctcgggcttc tacctcgcca
    19981 tgcacgatct ggagatccgc ggcgcgggcg aagtgctggg cgaatcgcaa agcggcgaca
    20041 tgcttgaagt cggcttcaat ctctacaacg acatgttgaa ggccgcggtc aaggcactgc
    20101 gggaaggcaa ggagccggac ctcagccagc cgctcggcgt gacgacggaa atcaatctgc
    20161 acatgcccgc gctgctgccg aacgactatt gctcggacgt gaacgtgcgc ctgtcgctct
    20221 acaagcggct ggccaactgc gaggacgagg acgaactggc cgtcatgcgc gaggaactcg
    20281 tcgaccgctt cggcccgctg ccggatgcag cgcaagcgct gctggaaagc catcgcctgc
    20341 gcatcctcag ccgatcgctc ggcatcgtgc ggctcgatgc gaccgccgaa caggtgcaat
    20401 tgcatttcgg cgacaagccg agcgtcgatc cggcgcgcat catcgaactc atccagaaaa
    20461 accggcactg gaaactcaac ggtcccgaca gactgcgcgt ggacatcgtt tccgtcgcgc
    20521 tcaaggatcg ggtacaaatg attcgcaaag tactcgaaat gctcaaagga aaaacccaat
    20581 gaacgaagcg actcacaacc tgatcattca ggggcccgac gcccaggaaa gcatcgcaca
    20641 aagcctcagc atgctgaccg gcgcgcgcgc agtgcagccg ctcggcccgc atgcgttccg
    20701 tctgcgtcat gcgcaccagc gccaggccgt gctcgacgta tgccgcggct ggcgcatcga
    20761 ccatgcctac gtaccgacca ccacccgact ggacgaccac aaattgctcg ccatcgacat
    20821 ggattccacg ctcatcaccg tcgagtgcat cgacgaactg gcggatttcg ccggcgtgaa
    20881 agcgcaggtg agcgaggtga cgcaagccgc catgcgcggc gagatcgact tcggcgaatc
    20941 gctcaagcgc cgcgttgc
//
LOCUS       DATGOU010000077         7737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_5086972, whole genome shotgun sequence.
ACCESSION   DATGOU010000077 DATGOU010000000
VERSION     DATGOU010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5086972"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VKZ48_04620"
     CDS             <1..205
                     /locus_tag="VKZ48_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HLU77174.1"
                     /translation="VILTGLLADSCVLFTANDAYLRRYRITVGADCVASIEPQHAAQA
                     LDHMRRNLKATIESAADVRFEEA"
     gene            complement(413..1144)
                     /locus_tag="VKZ48_04625"
     CDS             complement(413..1144)
                     /locus_tag="VKZ48_04625"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HLU77175.1"
                     /translation="MPTRRNTATRADAPAAQFPAFGDEQLRDEGRAQTAPAEEARIAA
                     PASARGSLDNLREAAMRCRACPLWRHATQTVFGEGASHAPLLVVGEQPGNSEDLAGHP
                     FVGPAGRLLEQAMSASGVDRDQVYLTNAVKHFKWELRGKRRLHKTPAQKEIDACLPWL
                     QAEIELVKPKVVLCLGATAARATLGGPVRINEHRGQSIRTPNGYEVVVTMHPAYVLRL
                     KAGAAEEAYNMMRSDIALAARIATG"
     gene            complement(1213..2331)
                     /locus_tag="VKZ48_04630"
     CDS             complement(1213..2331)
                     /locus_tag="VKZ48_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HLU77176.1"
                     /translation="MSESHSSEEEPIVVAPVEPLAEAVQAAETSKRIAWAVLLLATAY
                     AIQAAAGFVLSIVLAALFALLLAPIVKGLHRIGIPQPLGAAIVMLVLLALMGVLASNL
                     YEPVRKWATVGPRDLVVLEHKFRNLLRPVQSMKNVAEKVTEIASKGESGDETPRQVVV
                     AQSGPTLFARLQEAAVAFVTTVMLVYFLLASGDTFLRKAVRVIPRLRDKITAVEIARE
                     VQQQIGRYFMTIAMVSTVLGVVTGIVMWLLDMPTPLLWGVMAGVLNFILYLGPIISAV
                     VISAVSLFTFDETFRILLPPAVFILLHFIEGHIVEPYVMGGRFAMNPVVIFVWILLWG
                     WLGGAIGVLIAVPLLVAIRICASHIPAFSALAEIIAKD"
     gene            2492..2695
                     /locus_tag="VKZ48_04635"
     CDS             2492..2695
                     /locus_tag="VKZ48_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HLU77177.1"
                     /translation="MPECRMTAPGVHIQMLFYAFLFFVVACIAALAGFNDIAPSLAVI
                     ARLVAYAFLALAAITALIAIVRR"
     gene            3109..4362
                     /locus_tag="VKZ48_04640"
     CDS             3109..4362
                     /locus_tag="VKZ48_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HLU77178.1"
                     /translation="MRAQGRLLPPQEARALLARIGREGETDLLRRHLDSALAGVASPL
                     VPGNRVELLVDGPQTHESMFAAIRAARDHVNLQSYIISADEVGQELATLLRSKREQGV
                     RVNLLYDSAGGRDTPKEYFDGLRDADIAVCEFNPIDPTKTRGAWRINNRNHRKLLIVD
                     GAVAFTGGINISGVYSAGSFGSGTRSPSRGWRDTHARFEGPVATEAQRLFVDAWRSQR
                     HCDKLEEAHYFPEVAPRGTRVVRLVASAPDYGSETYATLLSALEHAERRAYLTYGYFA
                     PDERMLEAIKAAAQRGVDVQLVLPGFSDFWAPLAAGQSHYDALLESGVRIHERTDALL
                     HAKTAVIDGVWSTVGSTNLDWRSFVHNYEADLMVLSEDFGKALEELFAADVAHSQEIR
                     LEAWRNRGLRQRLKEWFARQWEYWL"
     gene            4613..4837
                     /locus_tag="VKZ48_04645"
     CDS             4613..4837
                     /locus_tag="VKZ48_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HLU77179.1"
                     /translation="MKGNTLFIGLMLGCAVSACSTWEGMSEREKGAAIGAGVGGVAGA
                     AATDGGILGTVGGAAIGGVVGHEVGKRQED"
     gene            complement(4882..5151)
                     /locus_tag="VKZ48_04650"
     CDS             complement(4882..5151)
                     /locus_tag="VKZ48_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77180.1"
                     /translation="MNELGIDGVDEGGLYNLDLGGSGPSPDRLQEAMRYLRERNRYIL
                     DKPVERRPNARPHLLTLIAEQIRREREMRDAMQRHNSFHQEQAHG"
     gene            complement(5245..5466)
                     /locus_tag="VKZ48_04655"
     CDS             complement(5245..5466)
                     /locus_tag="VKZ48_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77181.1"
                     /translation="MTQALWGVLLLSLATSAPGESDGANTRSAEARTPDAASRVVAAA
                     GVAESRVQPAKDARPTGKTSESYKAGTAR"
     gene            5693..7150
                     /locus_tag="VKZ48_04660"
     CDS             5693..7150
                     /locus_tag="VKZ48_04660"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007000205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HLU77182.1"
                     /translation="MALALELRHKQQLALTPQLQHALRLLQLSALEFAQEMGEALANN
                     PFLEEATPQKPAAPGAESTVPTVSTTATTTESPVEAPPAEPENEGDERFGFDSAAYAS
                     SSNAGESDGEWTDWSEPEPTLRAHLREQLLLSQMGERDRALAHLIIDALDDDGYLKQD
                     LEEIAALIATEHDVTADDLRSALRLVQTLDPPGVGARSLQECLTLQLTAKQANPRLTD
                     VACRIVQKHLTVLAAHELGKLQQLVGCDEGTLHDAIALIRSLDPKPGLRFGTGEARFV
                     IPDVLVSKIRGKWTVTMNPAVMPRLRINRSYADVVSGGQHSFMTKQLHEARWLLRSME
                     QRFITIQRVAEAIVGRQKAFFEYGEVAMKPLALKHIAAQLGVHESTVCRVTNNKFMAT
                     PRGVFEFKHFFSRTLATEAGGAASATAVRALLKEMIATEDPERPLSDAKLATLLSEQG
                     LRVARRTITKYRTLMRVPSVEVRRALGKQAARAAA"
     gene            7161..>7737
                     /locus_tag="VKZ48_04665"
     CDS             7161..>7737
                     /locus_tag="VKZ48_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77183.1"
                     /translation="MAIIVSGWFETQSRADEAMTALIDEGFQYDEVHSFYVSPPGQHS
                     DLPLSDLSSHEHSEGTKEAGKGAVKGAAVGAAVGVAAGAVAAAATAPLGPAAVVAGAG
                     VGAYVGSLAGGAAASRDSRIEEATREEPIDRPAGAMVSVYTDRHGEARAIGVLRDQGA
                     RGIERAEGQWIDGKWADFDPHVPPRLIHDEEM"
BASE COUNT         1361 a         2466 c         2580 g         1330 t
ORIGIN      
        1 cgtgatcctc accggcctgc tggcggattc ctgtgtgctg ttcacggcca acgatgcgta
       61 cctgcgccgc tatcgcatca cggtcggcgc ggactgcgtc gcttccatcg aaccgcagca
      121 tgcggcgcag gcgctcgatc atatgcggcg caatctcaag gcgacgatcg agtccgccgc
      181 cgacgttcgc ttcgaggaag cttgaccggc acgggtgttg cagtctgtct cccgcttcaa
      241 tgactaccgt gcgagtcgtg atgaacttct acgcgcatac gccggaaccg acgcctgccg
      301 atccggagga gccgcacaaa gtgccggtcg agccgccgcc cgagccgccc gatcccaatc
      361 cggttgagca agacgacgtg cacgcgcgga acgcgccgag cgccggcaga cctcatcccg
      421 ttgcaatccg cgccgcaagt gcgatgtcgg accgcatcat gttgtaggcc tcttcggccg
      481 cgcctgcctt caagcgcagc acgtaggcgg ggtgcatcgt caccaccacc tcgtagccgt
      541 tgggcgtgcg gatgctctgg ccgcgatgct cgttgatgcg aaccgggccg ccgagcgtgg
      601 cgcgtgcggc cgtggcgccg aggcagagca ccaccttcgg tttgaccagt tcgatttccg
      661 cctgcagcca cggcaggcag gcatcgatct ccttctgcgc gggcgttttg tgcagccgcc
      721 gcttgccgcg caattcccat ttgaaatgct tgacggcgtt ggtgagataa acctgatcgc
      781 gatcgacgcc gctcgcactc attgcttgtt cgagcagtcg gcccgcaggt ccgacaaaag
      841 gatggccggc caggtcttcg ctgttgcccg gctgttcgcc gacgaccagc agcggtgcat
      901 gcgatgcgcc ttcgccgaac accgtctgcg tcgcatgacg ccatagcgga caggcgcggc
      961 agcgcatcgc ggcctcgcgc aggttgtcca gcgagccgcg cgcggaggcc ggcgcggcaa
     1021 tgcgggcttc ttcggctggc gccgtttgtg cgcgtccttc gtcgcgcaac tgttcgtccc
     1081 cgaatgcggg aaactgcgcg gcaggagcgt cggcgcgcgt cgctgtgttg cgtcgagtcg
     1141 gcatgctggg cacctgcgtt gagcgattca cagccacagc aagccgcatg cccctttgtg
     1201 caggcggtcg tgtcaatcct tggcgataat ttccgcgagc gcggagaagg cgggaatgtg
     1261 cgatgcgcag atgcggatgg ctaccagcag cggcacggcg atcagtacgc cgatggcgcc
     1321 gccgagccat ccccacagca gtatccagac gaagatgacc acgggattca ttgcgaagcg
     1381 tccgcccatg acgtacggtt cgacgatgtg cccttcgatg aagtgcagta ggatgaacac
     1441 cgcgggcggc agcagaatgc gaaaggtctc gtcgaaggtg aacaacgata cggcgctgat
     1501 caccactgcc gaaatgatcg gaccgaggta gaggatgaag ttgagcacgc ctgccatcac
     1561 gccccacaga agcggcgtcg gcatatcgag cagccacatg acgataccgg tgacgacacc
     1621 cagcaccgtg ctgaccattg cgatggtcat gaagtagcgg ccgatctgct gctgcacttc
     1681 gcgcgcgatt tccaccgccg tgatcttgtc gcgcaggcgc ggaatgacgc gcaccgcctt
     1741 gcgcaggaag gtatcgccgg aggcgagcag aaagtacacg agcatgacag tggtgacgaa
     1801 cgccaccgcc gcttcctgca gccgcgcgaa aagggtcggg cccgattgcg cgaccaccac
     1861 ctggcgcggc gtctcgtcgc cgctttcgcc cttcgacgcg atttccgtca ctttctcggc
     1921 cacgtttttc atcgactgca ccggccgcag cagattgcgg aacttgtgtt ccaggacgac
     1981 cagatcgcga gggccgacgg tcgcccactt gcgcaccggc tcgtacaggt tggaggcgag
     2041 gacgcccatc agcgccagca ggacgagcat cacgattgcg gcacccagtg gttgcgggat
     2101 gccgatgcga tgcaggcctt tgacaatggg cgcaagaagc agcgcgaaca gcgccgccag
     2161 gacgatcgac agcacgaagc ccgccgccgc ctggatggcg taggccgtgg cgagcagcag
     2221 caccgcccag gcgatgcgct tgctcgtctc cgccgcctgc accgcttcgg cgagcggttc
     2281 caccggggcg acgacgatcg gttcctcttc gctgctgtgc gattcgctca tgggtcgggg
     2341 aggtgtggcg accgtgcgga agcggcggtg tcctatacgg gcaagcgcag tgccggaatg
     2401 agtgtcgggg taaggcgacg gcaggcacag gcgttgctgt aaaaaagcga tcccgaaagg
     2461 ggcgaaatgc tgcgaatgcc tttgacaccg catgcctgaa tgccgcatga ccgcacctgg
     2521 ggttcacatt caaatgctgt tctacgcctt tctttttttc gtcgttgcct gcatcgcggc
     2581 gctcgccggc ttcaacgaca ttgcgccctc gctcgccgtc attgcccggc tggtggccta
     2641 tgcttttctt gcgctggccg caattaccgc gctgatcgcc atcgtgcgtc gctgaatgcg
     2701 cggcacgggc gttgctctca gtcggatcgt agggacgcat caaggattcg cccgctcgtc
     2761 ctgatcgggc gcctcaccgg gaagccagca tgcacaacga cgatacgaag aagaagcaaa
     2821 ccgatcagcc cgaggagcag aagggcaagg cctgggaacg cgggaacgac ggcgcgccgc
     2881 ggcggcgccg cggaccatac ggcccgatcc ggagcggacg ctacgcacag cgcacaccgc
     2941 gaaatccggg ctgaactgtc aaccctgacg acacgctgcg cgcggcgagg cagggcgcac
     3001 cgtcggcgcg tccacgtcgc gctctttgcg ctactgagcg ccggatgtgc ggtacgcagc
     3061 gatccgctgc cgccgtcggt cgcgctgcaa cccgatgcgg cgccggccgt gcgtgcgcag
     3121 ggacggttgc tgccgccgca ggaggcgcgt gcgctgcttg cgcgcatcgg ccgcgaaggc
     3181 gagaccgatc tgctgcgccg ccatctggac agcgcgctgg ccggcgttgc cagtccgctc
     3241 gtgcccggca accgcgtcga gttgctggtg gacgggccgc aaacgcacga gtccatgttc
     3301 gccgccatcc gcgcggcgcg cgatcacgtc aatctgcaga gctacatcat ctccgccgat
     3361 gaagtcggac aggaattggc gacgctgttg cgcagcaagc gcgaacaggg cgtgcgcgtc
     3421 aacctgctgt acgacagcgc cggcggacgc gacacgccga aggagtattt cgacggcttg
     3481 cgtgatgcgg acatcgcggt gtgcgaattc aatccgatcg atccgaccaa aacgcgcggc
     3541 gcctggcgca tcaacaaccg caatcaccgc aagctgctga tcgtcgacgg tgcggtggcg
     3601 ttcaccggcg gcatcaacat cagtggcgtg tattcggcgg gctccttcgg cagtgggacc
     3661 cggtcgccgt cgcgcggctg gcgcgatacc catgcgcgct tcgaagggcc ggtggcgacg
     3721 gaagcgcagc ggctgttcgt cgacgcctgg cgcagccagc gtcactgcga caaactcgaa
     3781 gaggcgcatt actttccgga ggtcgcaccg cggggcacgc gcgtcgtgcg tctggtcgcc
     3841 agcgcgcccg attacggcag cgagacctat gccacgctgc tgtccgcgct cgagcacgcg
     3901 gagcggcgtg cctatctcac ctacggctac ttcgcgccgg acgagcgcat gctcgaggca
     3961 atcaaggcgg ccgcgcaacg cggcgtggac gtgcaactgg tcctgcccgg cttcagcgat
     4021 ttctgggcgc cgcttgcggc ggggcagtcg cattacgacg cgctgctcga aagcggcgta
     4081 cgcatccacg agcgcaccga cgccttgctg catgcgaaga ctgcggtgat cgacggcgta
     4141 tggtctacgg tcggctcgac caacctcgac tggcgcagct tcgtgcacaa ctacgaggcg
     4201 gatctgatgg tgctgagcga ggatttcggc aaggcgctgg aagaactgtt cgccgccgac
     4261 gtcgcgcatt cgcaggaaat ccggttggag gcctggcgga accgcggtct ccgccagcgc
     4321 ctcaaggagt ggttcgcgcg gcagtgggag tattggctgt gagtagagag tggtgagtgg
     4381 tgagtcgaga gcgaagtacg gcgcctcacg gcgcttgtgg attcccgctt acgcgggaat
     4441 gaccgcaaac gcatccaccc tcttaccagc caagccccgg ccgcttcgta cttctactgc
     4501 ttgtgcgctc gactctcggc tcagcgctct cggctctcgg ctctcggctg ctttccccgg
     4561 gcccatcggt tgcaaacgtc aatatgtgtt aaatcgaaag aggtttgctg ccatgaaagg
     4621 caacacgctg tttataggac tgatgctggg ctgcgccgta agcgcgtgca gcacctggga
     4681 aggcatgtcc gaacgggaga agggcgcggc catcggcgcg ggtgtgggcg gtgtggccgg
     4741 tgcggccgcc accgacgggg gcatactggg cactgtgggc ggcgctgcca tcggcggcgt
     4801 ggtggggcac gaagtcggca agcgccagga agactgaggc ggtctgccgc cggcggcggc
     4861 agaccgttac gacctcacaa ctcagccgtg cgcctgctcc tgatggaagc tgttgtggcg
     4921 ctgcatggcg tcgcgcattt cccgctcgcg gcggatctgt tcggcgatga gcgtcaacag
     4981 atgcgggcgt gcgttggggc ggcgctccac cggtttgtcg aggatgtagc ggttgcgctc
     5041 gcgcaggtag cgcatcgctt cctgcagacg gtcggggctc ggacctgaac cgcccagatc
     5101 gaggttgtac agaccgcctt cgtcaacgcc gtcgatacct aattcgttca tgtctttctc
     5161 cgtccgtaga tgggactgtt gccggatggc gacaccgttt ggcctcccgc agtccagtcc
     5221 atacctgcgt gttgaagtgc tgcgctaccg agccgtgccg gctttgtagc tttcgctcgt
     5281 tttgccggtc ggacgtgcat ctttggccgg ctgcacgcgc gattcggcga cgcccgcagc
     5341 cgctaccacc cggctggcgg cgtctggcgt acgagcttcg gccgaacgcg tgttcgcgcc
     5401 gtcgctctcg cctggggcgc ttgtcgcgag cgacagcaac agcacgcccc acaaggcttg
     5461 cgtcatcggc aatctcctcc acgagttacc cgtcgatgtc tgacaagcag tgattgtgcc
     5521 gttcggccga tcgcgcgcat ggcacaggtc ttgcaaccga gggatttcgg agcggtagcc
     5581 gaggcgctcg gggccggtcc tttcgaagta cgtttcgctc gcgatgcggg tggtccgaaa
     5641 gccgtactga aacaaccaat ctacaaatgc gcgccgcgtt taggaggatc acatggcact
     5701 cgccttggaa ttaaggcaca agcagcagct tgctctcacg ccacaactgc agcatgcgtt
     5761 gcggctgctg caactgtccg cgctggagtt cgcccaggaa atgggcgagg cgctggccaa
     5821 caatcccttt ctcgaggaag cgacaccgca gaagcccgct gcgccgggcg cggagtcgac
     5881 cgttccgacg gtaagcacaa cggcaacgac gacggaatcg ccggtcgagg cgccgcccgc
     5941 ggagcccgag aacgagggcg atgaacgctt cggcttcgat tcggccgctt atgcgagcag
     6001 cagcaacgcc ggcgaatcgg acggcgaatg gaccgactgg agcgaaccgg aaccgacgct
     6061 gcgtgcgcat ctgcgcgagc aactgctgtt gtcgcaaatg ggtgagcggg atcgtgcgct
     6121 ggcgcacctg atcatcgatg cgctcgacga cgacggctat ctgaaacagg atctcgaaga
     6181 gatcgccgca ctcatcgcca ccgagcatga cgtgaccgcc gacgatctgc ggtcggcgtt
     6241 gcggctggtg cagacgctcg atccgcccgg cgtgggcgcg cgctcattgc aagaatgtct
     6301 gacactgcaa ctgacggcca agcaggccaa tccgcgcctc accgatgtgg cgtgcaggat
     6361 cgtacaaaag catctcaccg tgctcgccgc acacgaactc ggcaagctgc agcaactggt
     6421 cggctgcgac gaaggcacgc tgcatgacgc catcgcgctg atccgttcgc tcgatcctaa
     6481 gccgggcctg cgcttcggta cgggagaggc gcgcttcgtc attcccgacg tcctggtcag
     6541 caagatccgc ggcaagtgga ccgtcaccat gaacccggcg gtgatgccgc ggctgcgcat
     6601 caatcgcagc tatgccgacg tcgtctccgg cggacagcat tcgtttatga ccaagcagtt
     6661 gcacgaggcg cgctggctgc tgcgcagtat ggagcagcgc ttcatcacca ttcagcgcgt
     6721 ggccgaggcg atcgtcggcc ggcagaaggc cttcttcgaa tacggtgaag tggcgatgaa
     6781 gccgctggcg ctcaagcaca tcgccgcgca actgggggtt cacgaatcca ccgtctgccg
     6841 cgtgaccaac aacaagttca tggcgacgcc gcgcggggtg ttcgagttca agcacttctt
     6901 ctcgcgcacg ctcgccaccg aggccggcgg tgcggcatcc gccaccgcgg tgcgtgcgct
     6961 gctgaaggaa atgattgcga ccgaggatcc cgagcgtccg ttgtccgatg cgaaactcgc
     7021 gaccctgttg tcggaacaag gtctgcgcgt tgcgcgtcgt acgatcacca agtaccgcac
     7081 gctgatgcgc gtacccagcg tcgaagtgcg acgcgcactc ggcaagcagg cggcgcgcgc
     7141 ggcggcttaa aggagacacg atggcgatta tcgtatccgg ctggttcgaa acgcagtcgc
     7201 gcgccgacga ggcgatgacg gcattgatcg atgaaggctt tcaatacgac gaggtgcata
     7261 gcttctacgt cagcccgccc ggacagcaca gcgatctgcc gctgtccgat ctgagcagcc
     7321 acgaacacag cgaaggcacc aaggaagccg gcaagggtgc ggtgaagggc gccgcggtcg
     7381 gcgccgcagt cggtgtggcc gctggggcgg ttgccgctgc tgccactgca cccttgggcc
     7441 ccgcggcggt cgtggccggc gccggtgtcg gcgcttatgt gggttcgctg gcgggcggtg
     7501 ccgcggcatc gcgcgacagc aggatcgaag aggcgacgcg cgaggagccc atcgaccggc
     7561 ccgccggcgc gatggtatcg gtctatactg accgtcacgg cgaagctcgc gcgatcggcg
     7621 tgctgcgcga tcagggcgca cgcggcatcg aacgtgcgga aggccagtgg atcgacggca
     7681 agtgggccga tttcgatccg catgtgccgc cgcgactaat ccacgatgag gaaatgc
//
LOCUS       DATGOU010000078        21260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_5531290, whole genome shotgun sequence.
ACCESSION   DATGOU010000078 DATGOU010000000
VERSION     DATGOU010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 21260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21260
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5531290"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..146)
                     /locus_tag="VKZ48_04670"
     tRNA            complement(60..146)
                     /locus_tag="VKZ48_04670"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(110..112),aa:Leu,seq:taa)
     gene            complement(386..459)
                     /locus_tag="VKZ48_04675"
     tRNA            complement(386..459)
                     /locus_tag="VKZ48_04675"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(425..427),aa:Cys,seq:gca)
     gene            complement(543..618)
                     /locus_tag="VKZ48_04680"
     tRNA            complement(543..618)
                     /locus_tag="VKZ48_04680"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(583..585),aa:Gly,seq:gcc)
     gene            complement(657..1229)
                     /gene="pgsA"
                     /locus_tag="VKZ48_04685"
     CDS             complement(657..1229)
                     /gene="pgsA"
                     /locus_tag="VKZ48_04685"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HLU77184.1"
                     /translation="MPLNLPIVLTWIRVGMIPLFVAIFYLPAGWLDLSDRNLVATVIF
                     TAAAVTDWLDGYLARRWNQTSAFGAFLDPVADKLIVAAALIVLVDLTRVDPILAFIII
                     GREITISALREWMAKVGETKSVAVNALGKIKTVSQLVAIPMLLYHDRIGSLDPQRLGT
                     WLIWAAAVLTLLSMFYYLKLAAHAFKRNAA"
     gene            complement(1231..3030)
                     /gene="uvrC"
                     /locus_tag="VKZ48_04690"
     CDS             complement(1231..3030)
                     /gene="uvrC"
                     /locus_tag="VKZ48_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HLU77185.1"
                     /translation="MFDSATFLAGLPHLPGVYRMLGAAGEVLYVGKARDLKKRVSSYF
                     QKSGHGPRISLMISQIASVEITVTRSEAEALLLENNLIKSLTPRYNILFRDDKSYPYL
                     MISGHTFPRLAFHRGPLDKKNSFFGPYPNAGAVRESIQSLQKVFRLRTCEDSVFMNRT
                     RPCLLHQIKRCSAPCVGLVDEQSYRADVHNAIMFMEGREDDVRSELAQRMQEAADRLD
                     YEQAAVFRDRIQSLSNVQGRQYVDVGKALDADAVACVVERGMTCVNLVMVRNGRYLGD
                     KSFFPQHAEDHSPAEVVGAFLAQHYAQSPVPPVIVVPEAVETEELGALLSDLAGHTVQ
                     LSNHPITERRAWLDMAQRNAEHALAQRVREQGTQELRLQAMREALDLPSGAQRVECFD
                     ISHTQGEATVAACVVYDQLGMRPAEYRRYNITGVTPGDDYGAMRSVLQRRYEKVTRGE
                     GVVPDLIMIDGGRGQVNAARGVLEDLGLGDVAMIGVAKGPERKPGLEELILADGKRSL
                     HLPPDHPGLHLIQQIRDEAHRFAITGHRARRAKARITSSLEGIAGVGAKRRRQLLERF
                     GGLKGVMAASVEDLAQVEGISRTLAERIYRELH"
     gene            complement(3055..3693)
                     /locus_tag="VKZ48_04695"
     CDS             complement(3055..3693)
                     /locus_tag="VKZ48_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HLU77186.1"
                     /translation="MSGGEVFDIDCVQCPRLAAFLATVRQEHPGYHARPVPPFGDAQA
                     QLLVVGLAPGMHGANRTGRPFTGDYAGILLYETLYKYGFADRVGTADPDDGLTLVGCR
                     ISNAVKCLPPANKPNPDEIKRCNQYLAAELTACPPRAVLALGRIAHDAVLMALGLKAS
                     GYRFGHHAVHDLPDGLRLYDSYHCSRYNTQTRRLTTDMFEAVFADIAAYLGR"
     gene            complement(3690..4319)
                     /locus_tag="VKZ48_04700"
     CDS             complement(3690..4319)
                     /locus_tag="VKZ48_04700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HLU77187.1"
                     /translation="MSGHHHHHHHRAGRHLAFGLAITFGFGLVEAVAGWWSGSLALIS
                     DAGHMLSDSFSLGLAAAAERISHRQGTGQGELHRAELWAALINTLLMAAIVLWIVYEA
                     IDRLQSPQPVMGEVVIGIAFIGIAVNVAVAYIISHGEQTLNTRAAMLHVMGDLAGSVV
                     ALISGITIYFGGWLAVDPILSLVVALLIGFATLNLLREALSAARGRRGA"
     gene            complement(4316..5341)
                     /gene="nagZ"
                     /locus_tag="VKZ48_04705"
     CDS             complement(4316..5341)
                     /gene="nagZ"
                     /locus_tag="VKZ48_04705"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HLU77188.1"
                     /translation="MSLALPLGPVMVDVAGHILTDAERDMLRHPLVGGVILFARNYQS
                     PEQLKALTAAIRAVRTPALLIGVDHEGGRVQRFREGFTCLPPMRSIGRLYDRDPALAL
                     HLARATGLVLAAELRAHGVDFSFTPVLDLDFGCSRVIGDRAFHSRPDAVAQLAGALIE
                     GLGLLGMGAVGKHFPGHGGCEADSHVAIPVDRRSLAEIEQNDMQPFKALFARGLTAVM
                     PAHVIYPEVDAQPAGFSRRWLQDILRGQLGFDGMIFSDDLSMEGASVAGGPLARAQAA
                     LAAGCDMALVCNKPESARQVLEGLEALPVDAARLERLRRPTQADATAVRQAHADVNDA
                     LRAGVLV"
     gene            complement(5338..5724)
                     /gene="acpS"
                     /locus_tag="VKZ48_04710"
     CDS             complement(5338..5724)
                     /gene="acpS"
                     /locus_tag="VKZ48_04710"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HLU77189.1"
                     /translation="MIVGTGMDLVEIPRIEAALARHGVRFAERILTDLELRRFHAHIK
                     PAAYLAKRFAAKEAFSKAMTTGMRWPVSWRNIGVVNHPSGCPCFDLAPELDALVRERG
                     ITGMHVTITDERSMAAAYVVLEGLSA"
     gene            complement(5727..6488)
                     /locus_tag="VKZ48_04715"
     CDS             complement(5727..6488)
                     /locus_tag="VKZ48_04715"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HLU77190.1"
                     /translation="MIELGVNIDHVATLREARRTYEPDPVWAAVEAHLGGADGITVHL
                     REDRRHIQDEDVRRLRELTHIKLNLEMAATEEMIGIATRVRPEMAMLVPEGRHEITTE
                     GGLDILAQETRLHEAVARLADAGIVVSVFIDADLRQVEAAKRIGASVCEVHTGPYAHA
                     FHSQGRDPESAAVVKELETLRQTGAAIRELGMRFNAGHALNYFNVEPVAQLPGVRELH
                     IGHSIVSRAVYVGMREAVREMKALMVLASTTQRRS"
     gene            complement(6488..7219)
                     /gene="recO"
                     /locus_tag="VKZ48_04720"
     CDS             complement(6488..7219)
                     /gene="recO"
                     /locus_tag="VKZ48_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HLU77191.1"
                     /translation="MQQPAPLPRRRVENERAYVLHTWPYSETSLLVELYSRAYGRLPV
                     LAKGARRPRSALRGLLMAFQPLTLGWSGRGEVRTLNRCEWAGGLPLLKGDALLCGFYL
                     NELLMRLLAREDPHERLFDRYEQALAELQQPGDQARVLRAFERVLLKELGYAMTLDRD
                     AETGAPIEPDAFYDYEPERGPVRSRGEGRMMLRGSTLLAIAAEDYREPGVLQQCKQLM
                     RLLINHRLDQQPLHSRNVYRELQQL"
     gene            complement(7222..8115)
                     /gene="era"
                     /locus_tag="VKZ48_04725"
     CDS             complement(7222..8115)
                     /gene="era"
                     /locus_tag="VKZ48_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HLU77192.1"
                     /translation="MNEEFRCGTIAIVGRPNVGKSTLLNTLIGQKLAITSRKPQTTRH
                     RLLGIHTTDDAQYIFVDTPGFQTEHGGSLNRMLNRNVQLALHDVDAVMWVVEAGTLNA
                     RDRKVAELLPADVPAVMVVNKIDRIEDRAQLLEFLRHCGETFDRGAIVPVSARLARNT
                     DELLAVLRKHLPAQPALYPADELTDRSERFLAAELIREKLFRLLGEEVPYGSAVVIDQ
                     FQEEGRLRRIHASIVVEKAGHKAIVIGKGGAKLKEIATAARIDMERLFDAKVFLEIWV
                     KVRDGWSEDDSMLRSFGYDAG"
     gene            complement(8162..8818)
                     /gene="rnc"
                     /locus_tag="VKZ48_04730"
                     /pseudo
     CDS             complement(8162..8818)
                     /gene="rnc"
                     /locus_tag="VKZ48_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
     gene            complement(8845..9192)
                     /locus_tag="VKZ48_04735"
     CDS             complement(8845..9192)
                     /locus_tag="VKZ48_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HLU77193.1"
                     /translation="MHNKQRGMGLVGIFLLLVGIVLVAVLGMKVGPVYIEYFTVKKMV
                     ADIANSGESSPQEIRASFNRRAQIEYFDDISADDLIITKDELGFAYEKRIPLIGNLTL
                     LIDFEGYSPRSNR"
     gene            complement(9204..9992)
                     /gene="lepB"
                     /locus_tag="VKZ48_04740"
     CDS             complement(9204..9992)
                     /gene="lepB"
                     /locus_tag="VKZ48_04740"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015421592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HLU77194.1"
                     /translation="MNFALVLFLLLVVTGAMALADRLYFARRRAAGEREPWWIEYPKS
                     FFPVILIVFVLRSFIVEPFRIPSGSMIPTLQVGDFILVNKYTYGIRLPIINKKIIPLN
                     DPQRGDVIVFRYPVDPSYDYIKRVVGLPGDQVAYVDKHLSINGVAVPMEPAGDYLHRE
                     KISYSKRYIETIGGVRHEVLNDEEAPPGVPFVKQFPFRDNCAYNVDGFVCTVPPGHYF
                     LMGDNRDNSSDSRVWGFVPDSHIVGKAFFIWFNFGDLDRIGSFQ"
     gene            complement(10135..11928)
                     /gene="lepA"
                     /locus_tag="VKZ48_04745"
     CDS             complement(10135..11928)
                     /gene="lepA"
                     /locus_tag="VKZ48_04745"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013233931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HLU77195.1"
                     /translation="MQHIRNFSIIAHIDHGKSTLADRFIHRCGGLSEREMESQVLDSM
                     DLERERGITIKAQTASLQYKARDGQIYNLNLIDTPGHVDFSYEVSRSLAACEGALLVV
                     DASQGVEAQTVANCYTAIEQDVEVIPVLNKIDLPSAEPDRAKQEIEDVIGIDASDAIL
                     ASAKTGLGIDDILEAVIARVPPPKGDPAAPLQALIIDSWFDNYVGVVMLVRVMQGTLK
                     PKDRMLLMSTQATHLCEQVGVFTPKSQSRPQLSAGEVGFIISGIKELEAARVGDTITL
                     ADRPAAQALPGFKEIKPQVFAGLYPVESNQYDALREALTKLKLNDASLHFEPETSQAL
                     GFGFRCGFLGLLHMDIVQERLEREYDMDLITTAPTVVYEVVLRDGTLLQVENPARMPE
                     PSKIEEIREPIITTTIFVPQDYVGAVMTLCNNKRGVQRNMQYRGRQVVLTYEMPLNEV
                     VLDFFDKLKSASRGYASLDYEFLEYRAADMVKLDILINGEKVDALSLMVHRANAQYRG
                     RELAAKMRELIPRQMFDVAVQAAIGAHIIARETVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKKRMKQVGSVEIPQEAFLAVLQVDGK"
     gene            complement(12001..12291)
                     /locus_tag="VKZ48_04750"
     CDS             complement(12001..12291)
                     /locus_tag="VKZ48_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HLU77196.1"
                     /translation="MSATLAPPQLTLYGRTYCHLCTDMEAALEPLRQEFGFVIEVVDV
                     DTDPELEARYGELVPVLAHGDTRLCHYFLDEPAVRRHLVALAAEAAAVRGAS"
     gene            complement(12294..13268)
                     /locus_tag="VKZ48_04755"
     CDS             complement(12294..13268)
                     /locus_tag="VKZ48_04755"
                     /inference="COORDINATES: protein motif:HMM:NF015825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HLU77197.1"
                     /translation="MSRLWFAAIAAGSLLCGAAQAQELDAIQWLERMYSASKHLSYTG
                     TFLYQRGDHIETSRITRLVDEHGVRERLETLDGMPPREVVRDNDEVTCYLPGSMTMKV
                     DSRRETRPFPLVQGERLDDIALHYDVRKGVVDRIAGHEAQAIILDPRDELRYGHRLWA
                     DLETGLLLKAKTYNERDEQVEQFTFTHLQIGGPIDQKLVQSRFAGKGGNWRVETSAMA
                     EADLSKQGWRLKALPPGYRKVTELKRRVGEAEGVGHVVVSDGLAAVSVFIEPLQAGKV
                     NRTPMGPSRKGALNIYTRHIDNYLVTVIGEVPADSVHRIADAVEYKPH"
     gene            complement(13272..13823)
                     /locus_tag="VKZ48_04760"
     CDS             complement(13272..13823)
                     /locus_tag="VKZ48_04760"
                     /inference="COORDINATES: protein motif:HMM:NF015810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HLU77198.1"
                     /translation="MKEQVSALMDGEFDGLDCDGCINRLKQDPAAREVWDIYHLIGDS
                     LRGHVGVSLPASFAERLAQEPTVLAPRRRMSQRHPAWFALSAAASVAAVVAVGWMALP
                     MITQPQPTPVAQAPATLRLVQPTQMLSVAPAAVSTGVVANVTPVAGDISDYLIAHQRF
                     TPTSAMVGVAPYARTVSQDLEKR"
     gene            complement(13828..14427)
                     /gene="rpoE"
                     /locus_tag="VKZ48_04765"
     CDS             complement(13828..14427)
                     /gene="rpoE"
                     /locus_tag="VKZ48_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011883961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HLU77199.1"
                     /translation="MNDREIDQRLVERAQAGDKQAFDLLVTKYQRKLGRLLSRFIRDP
                     AEVEDVAQEAFVKAYRALPSFRGDSAFYTWLYRIGINTAKNYLVAMGRRAPTTTEYDS
                     EEAESFEDGEQLRDINTPESMLMTKEIGETVNRAMEELPEDLRTAITLREIEGLSYEE
                     IASIMNCPIGTVRSRIFRAREAIAAKLRPLLDTGKDRRW"
     gene            complement(14562..15086)
                     /locus_tag="VKZ48_04770"
     CDS             complement(14562..15086)
                     /locus_tag="VKZ48_04770"
                     /inference="COORDINATES: protein motif:HMM:NF018908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HLU77200.1"
                     /translation="MRSGLRAARGASDADPRGAVELLRFRRHQRHAYLQTDLTNALHL
                     SLRRPAALCAAVSFMHAAALACAVPALTGWPLALVAAGVALSWIAQWQRLWAQDVDLE
                     CRPDGGVALAGRARTLVGSALPLSWLAVIRLRDRAGHCEALVITPDRVDAAAFRRLRV
                     WLRWHRRAPSGASE"
     gene            complement(14972..16210)
                     /gene="fabF"
                     /locus_tag="VKZ48_04775"
     CDS             complement(14972..16210)
                     /gene="fabF"
                     /locus_tag="VKZ48_04775"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009913173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HLU77201.1"
                     /translation="MSRRRVVITGLGIVCPVGNTVAEAWSNITAAQSGITRVTKFDAT
                     PFASQIAGEVKNFDIGEYIPTKEARRMDTFIHYGMAAAIQAIKDCGIEVTPDNAERIG
                     CVIGSGIGGLPLIEETHEALTTGGARKISPFFVPGSIINMIAGNLSIMYGFKGPNYAL
                     VSACTTSNHCIGDAMRLIEYGDVDVAVAGGAESTVSPLGIGGFAAMRALSTRNDDPAT
                     ASRPWDQGRDGFVLGEGAGVLVLEEYEHAKKRGAKIYAELVGYGMSADAFHMTAPCED
                     GDGANRCMANALKNAGMNPDEIDYINAHGTSTPLGDVAETVAVKRCFGDHAKKLVVSS
                     TKSMTGHLLGAAGGIEAVFSTLAVHHQVAPPTANLVDIDPQCDLDYVPLEARPMQIRA
                     ALSNSFGFGGTNGTLIFKRI"
     gene            complement(16227..16469)
                     /gene="acpP"
                     /locus_tag="VKZ48_04780"
     CDS             complement(16227..16469)
                     /gene="acpP"
                     /locus_tag="VKZ48_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HLU77202.1"
                     /translation="MENIEQRVKKIVAEQLGVNEAEIKNESSFVDDLGADSLDTVELV
                     MALEEEFETEIPDEEAEKITTVQQAVDYINAHGKKA"
     gene            complement(16666..17406)
                     /gene="fabG"
                     /locus_tag="VKZ48_04785"
     CDS             complement(16666..17406)
                     /gene="fabG"
                     /locus_tag="VKZ48_04785"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018005157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HLU77203.1"
                     /translation="MLTGQVALVTGASRGIGKAIAMALGKAGATVVGTATTEAGATAI
                     GEYLQAANIKGRGAALNVNDPDAVTQLLAAIEGEHGGVSILVNNAGITQDNLAMRMKD
                     EEWDAVIDTNLKAVFRLSRAVLRGMMKARAGRIINITSVVGASGNPGQANYAAAKAGV
                     AGMSRALAREIGSRGITVNCVAPGFIDTDMTKALPEAQRDALLNQIPLGRLGRPEDVA
                     AAVVYLASPGAAYVTGTTLHVNGGMYMS"
     gene            complement(17409..18344)
                     /gene="fabD"
                     /locus_tag="VKZ48_04790"
     CDS             complement(17409..18344)
                     /gene="fabD"
                     /locus_tag="VKZ48_04790"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HLU77204.1"
                     /translation="MKLAMVFPGQGSQSVGMLGAYGDAPEVREVLDIASEVLQQDIGK
                     LIAEGPAEELNRTVNTQPVMVTAGYAAYRVWQSLGGPEPSVVAGHSLGEYTALVVAGA
                     LKFEDALPLVRFRAQVMQEAVPQGAGAMAAILGLEDDAVREVCSTAAQNEVVEAVNFN
                     APSQVVIAGHAGAVHRAMELAREKGAKRAVALPVSAPFHSSLLAPAAARLREYLANVT
                     ISTPRIPVIHNFDVQRHPEGEAIKNALVQQADHAVRWVETVRTMAAEGVTHVAECGPG
                     KVLAPLVKRIADGVQGLALNDRAAIEQAIASLKEA"
     gene            complement(18381..19340)
                     /locus_tag="VKZ48_04795"
     CDS             complement(18381..19340)
                     /locus_tag="VKZ48_04795"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006158599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HLU77205.1"
                     /translation="MTYSRIIGTGSYLPARLVTNHDLAQTVDTSDEWITTRTGIRQRH
                     LAEEGQNTSDLALEASKQALETAGVRAEDLDLIIIATTTPDQVFPSTACLLQQRLGNK
                     GAMAFDMQAVCSGFVYALAVADSLIRAGQARTALVVGAETLSRILDWQDRTTCVLFGD
                     GAGAVVLRADDKPGILASAMHADGSYSGILAAPGKLCGGRIVGNPFVEMDGQAVFKFA
                     VKVLDEVAREAVDRANLRLDDVDWLIPHQANLRILDSTARRLGFAAERVVVTVDRHAN
                     TSAASIPLALDVAVRDGRIKAGHKVLLEGVGGGFTWGAVLVQM"
     gene            complement(19364..20353)
                     /gene="plsX"
                     /locus_tag="VKZ48_04800"
                     /pseudo
     CDS             complement(19364..20353)
                     /gene="plsX"
                     /locus_tag="VKZ48_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006764798.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
     gene            complement(20434..20613)
                     /gene="rpmF"
                     /locus_tag="VKZ48_04805"
     CDS             complement(20434..20613)
                     /gene="rpmF"
                     /locus_tag="VKZ48_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HLU77206.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDSLSNPPLAVEPSTGETHLRHHISP
                     SGYYRGKKVVQTKND"
     gene            complement(20719..21210)
                     /locus_tag="VKZ48_04810"
     CDS             complement(20719..21210)
                     /locus_tag="VKZ48_04810"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HLU77207.1"
                     /translation="MSQAEQIDGLEFARECGRCSGQLVLDALSRLAELGCRAVRVDYV
                     IEGARNAAEQLCLRVDVVGRLELECQRCLEPLDYAVEVHSELELAVDPGFIETADDDV
                     DRVLATHSMDVVSLVEDELILDLPMVPMHAHCATPVEAKADKLSPFAVLASLKKKAGD
                     PGN"
BASE COUNT         3769 a         6897 c         6667 g         3927 t
ORIGIN      
        1 caagaagaaa caagaagaaa cgataagaaa cagaaagcaa tataactgct tgtttctatt
       61 ggtgcccgga gccggactcg aaccggcatg accttgcggt cgagggattt taagtccctt
      121 gcgtctacct gtttcgccat ccgggcctct cacctcttta cccgcgatta agatcgcgag
      181 ccagcgcagc tatgcgagcg gggggcagag tatgccattg cccctatggc cgccgttgcg
      241 ctaacgctcg ataagaaggc ttgccgaagc ccgcgtacgc ataccgatca cctcaccatc
      301 gcgagccagc gcagccgagc aagaaagtat gccattgcaa cagctcggac aacgaagcgt
      361 ccgtcacacc agaggagtcg aaggttggag gcgggggtcg gaatcgaacc ggcgtccacg
      421 gctttgcagg ccgctgcata accactctgc caccccgccg tagaggcgtc gcgcccggct
      481 gacggccgat gaagcgcgaa aactaaaaag ggaaagcagg acgccttccc ttgcatgcaa
      541 actggagcgg gagacgagtc tcgaactcgc gacctcaacc ttggcaaggt tgcgctctac
      601 caactgagct actcccgcag agcgaggccg cattctacgc agccgcctca agccggtcaa
      661 gcagcgttgc gcttgaatgc atgcgcggcg agcttgagat agtagaacat cgacagcagc
      721 gtcagcacgg ccgcggccca gatcagccag gtgcccagcc gctgcggatc gagcgagccg
      781 atgcggtcgt gatagagcag catggggatc gccaccagtt gcgacaccgt cttgatcttg
      841 cccagcgcat tgaccgccac gctcttggtc tcccccactt tggccatcca ttcgcgcagc
      901 gccgagatgg tgatttcgcg gccgatgatg atgaaggcca ggatcgggtc cacgcgggtc
      961 agatcaacca gcacgatcag cgcagcggcg acgatgagtt tgtcggcgac gggatcgagg
     1021 aaggcgccga atgcggaggt ctggttccag cgccgcgcaa gatatccgtc cagccagtcg
     1081 gtcacggcgg ccgcagtaaa gatgacggtg gcgacgaggt tcctgtccga caggtccagc
     1141 caaccggccg gcagatagaa gatcgcaacg aagagcggga tcattcccac ccggatccat
     1201 gtcagcacga tcggcagatt caacggcact tcagtgcaac tccctgtaga tgcgctcggc
     1261 cagggtccgg ctgatacctt cgacctgcgc caaatcctcc acgctcgcgg ccatcacgcc
     1321 tttgaggccg ccgaagcgtt ccagcaactg ccgccggcgc tttgcaccca cgccggcgat
     1381 cccttccagg ctcgacgtta tacgcgcttt cgcgcgccgc gcgcggtgcc cggtaatggc
     1441 gaaccgatgc gcctcgtcgc gaatctgctg gatcaaatgc aaacccggat gatccggcgg
     1501 caaatgcaga ctgcgcttgc cgtcggccag gatcagttcc tccaaccccg gcttgcgctc
     1561 cggccctttc gcgacgccga tcatcgcgac gtcgcccagg cccagatctt ccagcacgcc
     1621 gcgagccgca ttcacctggc cgcgtccgcc gtcgatcatg atcagatcgg gcaccacgcc
     1681 ttcgccgcgc gtcacttttt cgtaacgccg ctggaggacg ctgcgcatgg cgccgtagtc
     1741 gtcgcccggc gtaacgccgg tgatgttgta gcggcggtat tccgcaggcc gcatgccgag
     1801 ctgatcgtac acgacacagg ctgccacagt cgcctcgcct tgtgtgtgac tgatgtcgaa
     1861 acactcgaca cgttgcgcgc cgctcgggag gtcgagcgct tcgcgcatcg cctgcagccg
     1921 caactcctgc gtgccctgct cccgcacccg ctgcgccagc gcgtgttccg cgttgcgttg
     1981 ggccatgtcg agccaggcgc gccgctcggt aatcgggtga ttgctcagtt ggacggtatg
     2041 gccggccaag tcggacagta gcgcgcccag ttcctccgtc tcgactgcct ccggcaccac
     2101 gatgacgggc ggcacggggc tttgcgcgta atgctgcgca agaaaggcgc cgacgacttc
     2161 ggccggactg tgatcttcgg cgtgctgggg aaagaaactc ttgtcgccca gatagcggcc
     2221 gttacgcacc atgacgagat tcacgcaggt catgccccgt tcgaccacgc aggccacggc
     2281 gtccgcgtcg agcgccttgc cgacatccac gtattgcctg ccctgaacgt tggacagcga
     2341 ttggatgcga tcgcggaaca cggctgcctg ttcgtagtcg agccgatcgg ccgcctcctg
     2401 catgcgttgc gccagttcgc tgcgcacatc gtcctcacgc ccttccatga acatgatggc
     2461 gttgtgcacg tccgcccgat agctctgctc gtccaccagg cccacgcaag gcgccgaaca
     2521 gcgcttgatc tggtgcagca gacagggccg cgtccggttc atgaacaccg aatcctcgca
     2581 ggtgcgcagg cggaacactt tctgcagcga ctggatgctt tcgcgcaccg cgccggcatt
     2641 gggatacggg ccgaaaaagc tgttcttctt gtccagcggg ccgcggtgga aggccaggcg
     2701 cgggaaggtg tggccgctga tcatcagata cggataggac ttgtcgtcgc ggaacaggat
     2761 gttgtagcgc ggcgtcagcg acttgatgag gttgttttcc agcagcagcg cttcggcttc
     2821 cgagcgcgtc acggtgattt ccaccgacgc gatctgactg atcatcagcg agatgcgggg
     2881 gccgtgaccg cttttctgga aataggaaga gacgcgcttt ttcaggtcgc gcgccttgcc
     2941 cacgtacagc acttcgccgg cggcaccgag catgcggtac acaccgggca gatggggcaa
     3001 gccggcaaga aaagtggcgc tgtcgaacac tgcaagccga aggaggcgcg tcgcctaccg
     3061 gccgagataa gcggcgatgt cggcgaacac ggcctcgaac atatccgtgg ttagacgtcg
     3121 ggtctgggta ttgtatcggc tgcagtggta gctatcgtaa agccgcaggc catcgggcag
     3181 atcgtgcacg gcatggtggc cgaaacgata gccgctcgcc ttgagaccga gcgccatcag
     3241 caccgcatcg tgcgcgatgc gcccgagtgc gagcaccgcg cgcggcggac acgccgtcag
     3301 ttccgccgca agatactggt tgcagcgctt gatttcgtcc ggattgggct tgtttgccgg
     3361 cggcaggcat ttcaccgcat tgctgatgcg gcaaccgacg agcgtcagac cgtcgtcggg
     3421 atcggcggtg ccgacgcggt ctgcaaagcc gtacttgtac agcgtttcgt agagcaggat
     3481 gccggcgtaa tcgccggtga acggccgccc cgtacggttg gcaccatgca tgcccggcgc
     3541 aaggccgacg accagcaact gcgcctgcgc atcgccgaag ggcggcaccg ggcgcgcgtg
     3601 gtaaccgggg tgctcctgtc gcaccgtggc cagaaatgcg gccagacgcg gacactgcac
     3661 gcaatcgatg tcgaacacct ctcctccact catgcgccgc gcctcccgcg tgcggcggac
     3721 agggcctcgc gcagcagatt gagcgtggcg aatccgatca gcagcgcgac cacgagcgac
     3781 aggatgggat cgaccgcaag ccagccgccg aaatagatag tgatcccgga gatcagggcc
     3841 acgaccgagc cggccagatc gcccatcaca tgcagcatgg ccgcgcgcgt gttgagggtc
     3901 tgctcgccgt gggaaatgat gtaggcaacg gcgacattca cggcaatccc gatgaacgca
     3961 atgccgatca ccacttcgcc catcaccggc tgcggcgact gcagccggtc gatcgcctca
     4021 tagacgatcc acagcacgat cgccgccata agcagcgtat tgatcagcgc agcccagagt
     4081 tcggcgcgat gcaattcgcc ctgcccggtg ccctgtcggt gggaaatgcg ctcggccgct
     4141 gcggccagtc cgagcgagaa cgaatcgctc aacatgtggc cggcgtcgga aatcaaggcc
     4201 agggaaccgg accaccaacc ggccacagcc tccaccaggc cgaagccgaa ggtgatcgcc
     4261 agcccgaaag ccaggtggcg accggcgcga tgatgatggt gatgatgatg tccgctcaga
     4321 ccagcactcc cgcgcgcagg gcgtcattca cgtcggcgtg cgcctgacgg acggctgtcg
     4381 catcggcttg cgtgggacgg cgcaggcgtt ccaggcgcgc ggcgtcaacg ggcagcgctt
     4441 ccagcccttc cagcacctgc cgcgccgatt ccggtttgtt gcagaccagg gccatgtcgc
     4501 agccggcggc aagggccgct tgcgcgcgcg ccagcggccc gccggcgacg ctcgccccct
     4561 ccatcgacag gtcgtcgctg aaaatcatgc cgtcgaaacc caactgtcca cgcaggatgt
     4621 cctgcagcca gcggcgcgaa aagccggcgg gctgtgcatc cacttcgggg tagatgacgt
     4681 gcgcgggcat gaccgcggtg agaccgcgtg cgaacagcgc cttgaaaggt tgcatgtcgt
     4741 tctgttcgat ttccgccagg gaacgccgat cgacgggaat ggcgacatgc gaatccgctt
     4801 cgcagccgcc gtgaccggga aaatgcttgc ccaccgcacc catgccgagc aggcccagcc
     4861 cctcgatcag cgcgcccgcc agttgcgcca ccgcatcggg ccgggaatga aaggcgcgat
     4921 cgccgatgac ccggctgcag ccgaaatcca gatcgagcac gggcgtgaag ctgaagtcca
     4981 cgccatgcgc acgcagttca gccgccaata ccagccctgt ggctcgcgcc agatgcaatg
     5041 cgagtgctgg atcgcggtcg tacagccggc cgatgctgcg catggggggc aggcaggtga
     5101 agccttcgcg gaagcgctgc acgcggccgc cttcgtggtc cacaccgatc agcagcgcgg
     5161 gcgtgcgcac cgcccgtatc gccgccgtga gcgccttgag ttgctcgggc gactgataat
     5221 tgcgcgcgaa caggatgacg ccgccgacca gcgggtgcct cagcatgtcg cgctcggcgt
     5281 cggtcagtat gtgacctgcc acgtcgacca tgaccgggcc gagcggcaac gccaggctca
     5341 tgcgctaagg ccttccagga cgacgtaggc ggccgccatg ctgcgttcgt cggtaatggt
     5401 cacgtgcatg cccgtaatgc cccgctcgcg caccagcgcg tccagttcgg gcgccagatc
     5461 gaagcagggg caaccggatg gatgattgac cacgccgatg ttgcgccagc tcaccggcca
     5521 gcgcatgccg gtggtcatcg ccttggaaaa ggcctccttg gccgcgaagc gcttggccag
     5581 gtaagccgcg ggtttgatgt gcgcatggaa gcggcgcaac tcgaggtcgg tcaggatgcg
     5641 ctcggcgaaa cgcacgccgt gacgcgcaag cgctgcctcg atgcgcggaa tttccaccag
     5701 atccataccg gtaccgacga tcatgttcag ctccgccgct gcgtggtgct ggccagcacc
     5761 atgagcgctt tcatttcgcg caccgcttcg cgcatgccga catacactgc gcggctgacg
     5821 atcgaatgac cgatatgcag ttcgcgtacg ccaggcaatt gcgccacggg ctcgacgttg
     5881 aagtaattca gtgcgtgccc ggcattgaaa cgcatgccca gctcgcgtat cgccgcgccc
     5941 gtctgccgca gcgtctccag ttccttcacc accgccgcgc tctccgggtc gcgtccctgc
     6001 gaatgaaaag cgtgcgcata agggccggta tgcacttcgc acacggacgc gccgatgcgc
     6061 ttggccgctt cgacctgacg cagatcggcg tcgatgaaga cgctcaccac aatgccggca
     6121 tcggcaaggc gggcgacggc ttcgtgcaga cgcgtctcct gggcgaggat gtcgagcccg
     6181 ccttcggtcg tgatttcatg gcggccttcg ggcacgagca tggccatttc cggacgcacg
     6241 cgcgtggcga tgccgatcat ttcctcggtc gcggccattt ccagattcag cttgatatgc
     6301 gtcagctcgc gcagtcgccg cacgtcctcg tcctggatat ggcgccggtc ttcgcgcaag
     6361 tgcacggtaa tgccgtccgc accgcccaaa tgcgcctcca ccgccgccca tacgggatcg
     6421 ggctcgtagg tgcgccgcgc ctcgcgcagt gtggcgacgt ggtcgatatt gacgcccagt
     6481 tcgatcatca tagttgttgc aactccctgt agacgttgcg gctgtgcagc ggctgctggt
     6541 cgagccggtg attgatcagc agtcgcatca attgtttgca ctgctgcaac acacccggtt
     6601 cgcggtaatc ctccgcggca atggccagaa gcgtgctgcc gcgcagcatc atgcgccctt
     6661 ctccgcgact gcgtaccggc ccgcgctccg gttcataatc ataaaaggca tccggctcga
     6721 tgggggcgcc cgtttccgcg tcgcggtcga gcgtcatggc gtaacccagt tccttcagca
     6781 atacgcgctc gaaggcgcgc agtacgcgcg cctggtcgcc cggctgctgc aattcggcga
     6841 gcgcctgctc gtagcggtcg aacagtctct cgtgcggatc ctcgcgcgcc agcaggcgca
     6901 tgagcaattc attgagatag aaaccgcaca acagcgcgtc gcctttcagc agcggcagcc
     6961 cgcccgccca ttcgcagcgg ttgagcgtgc gcacttcgcc gcgccccgac cagcccagtg
     7021 tcaacggctg aaaggccatc aacaacccgc gcagcgccga acgcggccgc cgcgcgccct
     7081 tggcgagcac cggcagacgc ccgtacgccc ggctgtagag ctcgacgagc aggctggttt
     7141 cgctatacgg ccaggtgtgg aggacgtagg cgcgctcgtt ttcgacgcga cggcgcggca
     7201 ggggcgcagg ctgctgcatc gtcagccggc gtcgtagccg aagctgcgca gcatcgagtc
     7261 gtcttccgac cagccgtcgc gcaccttgac ccagatttcc agaaacacct tggcgtcgaa
     7321 cagccgttcc atatcgatac gggcggcagt ggcgatttcc ttcagtttgg cgccgccctt
     7381 gccgatgacg atggccttgt gtcccgcttt ctcgaccacg atggaggcgt ggatgcggcg
     7441 cagccgccct tcttcctgaa actggtcgat caccacggcg ctgccgtaag gcacttcctc
     7501 accgagcagg cgaaacagct tttcgcgtat gagctcggcg gcgaggaacc gttcgctgcg
     7561 atcggtcagc tcgtctgcgg gataaagggc cggttgcgcc ggcagatgct tgcgcagcac
     7621 ggcgagcagt tcgtcggtgt tgcgtgcgag ccgggcgctg accggcacga ttgcgccgcg
     7681 gtcgaaagtc tcgccgcaat ggcgcaggaa ttcgagcagt tgcgcgcgat cctcgatgcg
     7741 atcgatcttg ttcaccacca tgaccgcagg cacgtccgcg ggcagcagtt ccgccacttt
     7801 gcggtcccgc gcattcaatg tgcccgcctc gaccacccac atgaccgcat ccacatcatg
     7861 cagggccagt tgcacgttcc ggttcagcat gcggttgagc gagccgccgt gctcggtctg
     7921 aaagcccggc gtatcgacaa agatgtactg cgcatcgtcg gtggtatgga tgcccagaag
     7981 acgatggcgc gtggtctgcg gcttgcgcga ggtgatcgcc agtttctggc cgatcagcgt
     8041 attcagcagc gtggatttgc cgacgttggg gcggccgacg atggcgatcg tgccgcaacg
     8101 gaattcttcg ttcatgacct gcctggcggt tgggcgcccg cgctcaggcg ccggtaagcg
     8161 cttcgtacgc gcgacgcgcc gcttcctgct cggccaggcg gcggctggtg ccggtgccga
     8221 gcgtacgcgt gttgagcgcc ggaatgcagc actcgacctc gaaacgctga tcgtgcgctg
     8281 cgccgtgcgt cgccacgaca ttgtattgcg ggagcgggtg tttgcggctt tgcagccact
     8341 cctgcaactg cgttttggga tccttgtcgg cggtgacggt agggtcgagg gtgtcgagtt
     8401 gctcgccgta cagcgacagc accacacgcc gtgccgcctc ataaccgccg tccaggtaga
     8461 cggcgccgat cagcgcttcc acaccgtcgg cgaggatgga cggccgccgg aagccgccgc
     8521 ttttcagttc gccctcgccc aggcgcagaa aatcgcccag ttcgatgcgg gtggcgatgt
     8581 catgcagact ctgttcacgc accagactgg cgcgcaggcg cgacagctcg ccttcgcgca
     8641 gccgcacgaa gcggccatac agttcggccg cgacgatgaa gttgagaata ctgtcgccga
     8701 ggaattccag gcgctcgttg tgcggggtgc tgtggctgcg gtgcgtcagc gcctgcagca
     8761 gcagcttctg atcgttgaaa cggtacccga gacgggattg gaggcggtcg ttcttcaaaa
     8821 ctcgtacgtg ggcggcaccc ggggctacct gttgctgcgc ggactataac cttcgaaatc
     8881 gatgagcagg gtgagattgc cgatcagcgg aatgcgcttc tcgtacgcaa agcccaactc
     8941 gtccttggtg atgatcagat cgtccgcgct gatatcgtca aagtactcta tctgtgcgcg
     9001 gcgattaaac gaagcgcgaa tctcctgagg cgaactttcg ccgctgttgg cgatgtccgc
     9061 aaccattttc ttgacggtga aatactcgat atagaccggg ccgactttca ttcccagcac
     9121 ggcaacgagc acgattccga ccagcagcag gaatatccct accaggccca ttccccgttg
     9181 tttgttatgc atagtccctt cccctactgg aacgacccga tgcggtccag gtcgccgaag
     9241 ttgaaccaga tgaagaacgc tttcccgacg atatggctgt ccgggacgaa accccaaacc
     9301 cgactgtcgc tggagttgtc gcggttgtcg cccatcagga agtagtgtcc cggaggcacg
     9361 gtacaaacga aaccgtccac gttgtaggca cagttgtcac gaaaggggaa ttgcttcacg
     9421 aagggcacgc cgggcggtgc ttcttcgtcg ttcaggacct catggcgcac cccgccgatg
     9481 gtctcgatat agcgcttgga atagctgatc ttctcccggt ggagataatc gccggccggc
     9541 tccatcggta cggccacccc gttgatgctc aggtgcttat cgacataggc cacctgatcg
     9601 cccggtaggc cgacgacacg cttgatgtag tcgtacgacg ggtcgacggg atagcggaag
     9661 acgatcacgt cgccccgctg cggatcgttc agcggaatga tcttcttgtt gatgatgggc
     9721 aggcgaatgc cgtaggtgta cttgttcacc aggatgaagt cgcccacctg cagcgtcggg
     9781 atcatcgagc cggagggaat gcgaaatggc tcgacgatga acgagcgcag cacgaatacg
     9841 ataagaatga ccgggaagaa gctcttgggg tattcgatcc accacggctc ccgctcgcct
     9901 gctgcccgac gccgggcaaa gtagagccga tccgcgagcg ccatggcgcc cgtcacgacg
     9961 aggagcagaa aaagtacgag cgcgaagttc atgcctggag caccgttctg ttctaattgt
    10021 tagggaggct ctgattaagc ttgtttgatc gacgtcaccg ccagtccgtg cctattccgc
    10081 ggcacttaat caggtactcc gtcgtccgag cgcttggcgc gcacaggcgg aagctcactt
    10141 gccgtccacc tgcagcaccg ccagaaacgc ctcttgcggg atttctaccg agccgacctg
    10201 cttcatccgc ttcttgccgg ccttctgctt ttccagcagc tttttcttgc gcgtgatatc
    10261 gccgccatag cacttggcaa gcacgttctt gcgcagcgcc tttaccgtct cgcgcgcgat
    10321 gatgtgtgcg ccgatggccg cctgcaccgc gacgtcgaac atctggcgcg ggatcagttc
    10381 gcgcattttg gcggccaatt cgcggccgcg atattgcgcg ttggcacgat gcaccatcag
    10441 cgacagggcg tcgacctttt cgccgttgat gaggatatcg agcttcacca tgtcggcggc
    10501 gcgatactcc aggaattcgt aatcgagcga ggcatagccg cggctggccg acttgagctt
    10561 gtcgaagaag tcgagcacga cttcgttcaa cggcatttcg taagtcaaca cgacctggcg
    10621 accacgatac tgcatgttgc gctgtacgcc gcgcttgttg ttgcacagcg tcatgacggc
    10681 gccgacatag tcctgcggca cgaaaatcgt cgtggtgatg atcggctcgc gaatttcctc
    10741 gattttcgaa ggctccggca tccgcgccgg gttctcgacc tgcagcagcg tgccgtcgcg
    10801 cagcaccact tcgtacacca ccgtgggcgc ggtggtgatg agatccatgt cgtattcgcg
    10861 ctccagacgc tcctgcacga tgtccatgtg caacaggccg aggaaaccgc agcggaaacc
    10921 gaatcccaaa gcctgggacg tctccggctc gaaatgcagc gaggcatcgt tgagcttgag
    10981 cttggtcagt gcttcgcgca gcgcgtcgta ctggttcgac tcaaccgggt agaggcccgc
    11041 gaatacctgc ggcttgattt ccttgaagcc gggcagcgcc tgggccgccg gccgatccgc
    11101 cagggtgatg gtatcgccga cgcgtgccgc ctccagttcc ttgatgccag agatgatgaa
    11161 gcccacctcc cctgcggaaa gctgcggacg gctctgcgac ttgggcgtaa acaccccaac
    11221 ctgctcgcac aggtgcgtcg cctgcgtgga catcaacagc atccggtcct tcggcttgag
    11281 cgtaccctgc atcacgcgca ccagcatgac gacaccgacg tagttgtcga accacgagtc
    11341 gatgatgagc gcctgcagcg gtgcggccgg atcgcccttg gggggcggca cgcgcgcaat
    11401 caccgcttcc agaatgtcgt cgatgcccag gcccgtcttg gcgctggcga ggatggcgtc
    11461 actcgcatcg atgccgatga cgtcctcgat ctcctgcttg gcgcggtccg gctcggccga
    11521 gggcaggtcg atcttgttca gcaccggaat gacttcaacg tcctgttcga tggccgtgta
    11581 gcagttggcc acggtctgcg cctccacgcc ctggctggcg tcgacgacca gcagcgcgcc
    11641 ttcgcacgcg gcgagcgagc gcgatacctc ataggagaaa tcgacgtgtc cgggcgtgtc
    11701 gatcaggttc aggttgtaga tctggccgtc gcgcgccttg tactgcagcg acgcagtctg
    11761 cgccttgatg gtgatgccgc gctcgcgctc caggtccatc gaatcgagca cctgcgattc
    11821 catttcgcgt tcggacaggc cgccgcaacg atggatgaac cgatcggcga gcgtcgactt
    11881 accgtgatcg atatgggcga tgatggaaaa gtttctgatg tgctgcatgt tcgaagcgcg
    11941 gaccacaaaa aagggtgccg gcccagcacc ctcggagcgt aatgcaaggg tgggattcta
    12001 tcaggatgcg ccgcgcaccg cggctgcctc ggcggccagt gcgaccaagt gccggcgcac
    12061 cgccggctcg tccagaaagt agtggcaaag ccgggtgtcg ccgtgagcca gcaccgggac
    12121 gagttcgccg tagcgcgctt ccagctcggg atcggtgtcc acatcgacga cctcgatcac
    12181 aaagccgaat tcctggcgca ggggctccag cgcggcctcc atgtcggtgc acagatggca
    12241 ataggtccgg ccgtagagcg tcagttgcgg cggcgccagg gtcgcactca tcgtcagtgc
    12301 ggcttgtatt cgactgcgtc ggcgatccgg tgcacgctgt ctgccggcac ctcgccgatc
    12361 accgtcacca gataattgtc gatatggcgg gtgtagatgt tgagcgcgcc cttgcgcgac
    12421 gggcccatgg gcgtgcgatt caccttgccg gcctgtagcg gctcgatgaa cacggatacg
    12481 gccgcaagac cgtccgacac caccacatgc cccacaccct cggcttcccc gacgcggcgc
    12541 ttgagctccg tgaccttgcg atagccgggt ggcagcgcct tcaaccgcca gccctgcttg
    12601 ctcagatcgg cttccgccat cgccgaggtt tcgacccgcc agttcccgcc tttgcccgcg
    12661 aagcgcgact ggaccagctt ctggtcgatg gggccgccga tctgcaaatg ggtgaaagtg
    12721 aactgctcga cctgttcgtc gcgctcgttg tacgtcttgg ccttcaacag cagccccgtt
    12781 tccaggtcgg cccacaggcg atggccatag cgcagctcgt cgcgcgggtc gagtatgatg
    12841 gcctgcgcct cgtggccggc gatgcggtcc acgacgccct tacgcacgtc gtaatgcagg
    12901 gcgatgtcgt ccaggcgctc gccctgcacc aggggaaacg gccgggtttc gcgccggctg
    12961 tcgactttca tcgtcatgct gccgggcaga tagcaggtca cctcgtcgtt atcgcgcacg
    13021 acctcgcgcg gcggcatgcc atccagcgtt tcgaggcgtt cgcgcacgcc gtgctcatcc
    13081 accaatcgag tgatgcgcga agtctcgata tgatccccgc gctgataaag aaaggtgccg
    13141 gtatagctga gatgcttgct ggcgctgtac atccgctcca gccactggat ggcgtccagt
    13201 tcctgtgcct gagcggcgcc gcacagcaac gacccggcgg cgattgcggc aaaccacaaa
    13261 cgactcatgg ctcagcgctt ctcgagatcc tgggaaaccg tgcgcgcata aggtgcgacg
    13321 ccgaccatgg cggaggtcgg cgtgaagcgt tgatgcgcga tgagatagtc gctgatgtcg
    13381 ccggcaaccg gcgtgacgtt ggcaacgacc ccggtggaga cagcggccgg cgcgacgctc
    13441 aacatctgcg tgggctgcac gaggcggagc gtggcgggcg cttgcgctac gggtgtcggt
    13501 tgcggctggg tgatcatggg cagcgccatc cagcccaccg caacgaccgc ggcgacgctt
    13561 gccgcggcgg aaagcgcaaa ccaggcggga tgtcgttggc tcatgcggcg ccgcggcgcc
    13621 agtacggtgg gttcctgcgc cagccgttcg gcgaagctcg ccggcaggga gacgccaaca
    13681 tggccgcgca gtgagtcgcc gatcaggtgg taaatatccc agacctcgcg cgcggcgggg
    13741 tcctgtttca ggcggttgat acagccgtcg cagtcgaggc cgtcgaactc gccgtccatg
    13801 agggccgata cttgttcttt catcggttca ccaccttctg tctttacctg tgtccagcaa
    13861 cgggcgcaac ttcgccgcga tcgcttcgcg tgcgcggaaa atccgcgaac gcaccgtgcc
    13921 gatcgggcaa ttcatgatcg acgcaatctc ctcgtagctg agtccctcga tctcgcgcag
    13981 cgtgatcgcc gtgcgcaaat cctcgggcaa ctcttccatg gctcgattga cggtctcgcc
    14041 gatctccttg gtcatcaaca tgctctcggg cgtgttgatg tcccgcaact gctcaccatc
    14101 ctcgaagctc tctgcttctt cggaatcgta ttccgttgtc gttggcgcac gccgacccat
    14161 cgccaccaaa taattcttgg cggtgttgat gccgatccgg tacagccagg tgtagaacgc
    14221 gctgtcgccc ctgaacgagg gcaacgcgcg gtacgccttg acgaaagcct cctgggctac
    14281 gtcttccact tccgcggggt cccggataaa gcgggacagc aagcggccga gcttgcgctg
    14341 atacttcgtg acgagcagat caaacgcttg tttgtcgccc gcctgtgcgc gctcgaccag
    14401 ccgctggtct atttcccggt cattcattcg ccgccgcttc cctgagcccc gcttttttgc
    14461 gcttttaggg cgttattggt caggcggcag gagtataccg gagcaccctc ggcgtattgg
    14521 ccacgacctt gcagcaagag acaggtccct tattcgttag ttcattcgga agcgcccgag
    14581 ggcgcgcgac gatgccaacg aagccacact cgcaggcgcc tgaacgcggc cgcatcgacg
    14641 cggtccggcg tgatgacgag cgcttcgcaa tggcccgccc gatcgcgcag gcggatcacc
    14701 gccagccagg acagcggcag tgcgcttccc accagcgtgc gcgcccgccc cgcgagcgcc
    14761 acgcctccgt cgggccggca ctccaaatcg acgtcctgcg cccatagccg ttgccactgc
    14821 gcgatccacg atagcgcaac gccggccgcc accagcgcca gcggccagcc cgtcagggcc
    14881 ggcacggcac aggccagggc cgctgcatgc ataaacgaca cggccgcgca gagcgcggcc
    14941 gggcgtcgta gggaaagatg cagcgcgttc gtcaaatccg tttgaagata agcgtgccgt
    15001 tggtgccgcc gaaaccgaag gagttcgaca gcgccgcgcg gatctgcatc ggacgcgcct
    15061 cgagcggcac gtagtccaga tcgcactgcg gatcgatgtc gaccaggttg gcggtcggcg
    15121 gcgcgacctg atgatggacg gccagcgtcg agaacacggc ctcgatgccg cccgcggcgc
    15181 cgagcagatg cccggtcatg gatttggtcg aactcaccac cagcttcttg gcgtgatcgc
    15241 cgaagcagcg cttgaccgcg acggtttcgg ccacatcgcc cagcggcgtg gacgtgccgt
    15301 gcgcgttgat gtagtcgatt tcatcggggt tcatgccggc gttcttcagc gcattggcca
    15361 tgcagcggtt cgcgccatcg ccatcctcgc acggcgcagt catgtggaac gcgtccgcgc
    15421 tcatgccgta accgaccagt tcggcgtaga tcttcgcgcc gcgcttcttg gcgtgttcgt
    15481 attcctccag caccagcacg ccggcgccct cgcccagcac gaagccgtcg cgcccctgat
    15541 cccagggacg gctggccgtc gccggatcgt cgttacgcgt ggacagcgcg cgcatcgcgg
    15601 caaagccgcc gatgcccagc gggctcacgg tgctctccgc accgcctgcc acggccacgt
    15661 ccacgtcgcc gtattcgatc agccgcatgg cgtcgccgat gcagtgattg gacgtggtgc
    15721 aggcggagac cagcgcgtag ttcgggccct tgaagccgta catgatcgac aggttccccg
    15781 cgatcatgtt gatgatgctg cccggcacga agaaggggga gatcttgcgc gcgccgccgg
    15841 tggtcagcgc ttcgtgcgtc tcctcgatca gcggcaagcc gccgattccc gagccgatga
    15901 cgcagccgat gcgctccgca ttatcgggcg tgacctcgat gccgcaatcc ttgatggcct
    15961 gaatcgcagc cgccatgccg taatggatga acgtatccat ccggcgcgct tctttcgtgg
    16021 ggatgtactc gccgatatcg aagttcttga cctcgccggc gatctggctg gcgaacggcg
    16081 tggcgtcgaa tttcgtgacg cgggtgatac cggactgagc ggcggtgata ttcgaccagg
    16141 cctcggcgac cgtgttgccg acggggcaaa cgatgccgag accggtgata actactcgac
    16201 gacgggacaa aagcgacctc gtctgtttag gctttcttgc cgtgcgcgtt gatgtaatca
    16261 acggcttgct gcacggtcgt gatcttctcg gcttcctcgt cgggaatttc cgtttcgaac
    16321 tcctcttcca gtgccatgac gagttcgaca gtatccagcg aatcggcgcc cagatcgtcg
    16381 acaaacgacg attcgttctt gatctcggct tcgttcacgc ccagttgctc agccacgatc
    16441 ttcttgacgc gctgttcgat gttttccatg ggatcctctg tgttgtgttc tgggtaaatc
    16501 agagcggggc gcattgtagc agaactactt ggcgataccg ctcgcctgaa agtgctttaa
    16561 cagggcgctt gaccgacgcc ttccttcgag cgctttttgc cgactaagcg cttgattgcg
    16621 ctgtgctcgc ctgcactcat cgttcagcgc ctgcgcaggc ttctctcagc tcatgtacat
    16681 gccgccgttg acgtgcaggg tcgtgcccgt tacgtaggcg gcgcccggcg aggcgaggta
    16741 caccaccgcc gcagctacgt cctccgggcg tccaagacgt cccagcggaa tctggttcaa
    16801 cagcgcatcg cgctgcgctt cgggcaacgc tttggtcatg tcggtatcga tgaagcccgg
    16861 cgccacgcaa ttgaccgtaa tgccgcggct gccgatttcg cgtgccagcg cacgggacat
    16921 gccggccacg ccggctttgg ccgccgcgta gttggcctgg ccggggttgc cgctcgcacc
    16981 gaccaccgaa gtgatattga tgatgcgccc ggcgcgggct ttcatcatgc cgcgcagcac
    17041 cgcgcgggaa aggcggaaga ccgccttgag attggtatcg atgacggcat cccactcctc
    17101 gtccttcatg cgcatcgcaa gattgtcctg cgtgatgccg gcattgttga ccaggatgga
    17161 aacgcccccg tgttcgcctt cgatcgcagc caacagctgc gtgacggcgt cgggatcgtt
    17221 gacattcagt gccgcgccgc ggcccttgat gttcgcggcc tgcaggtatt cgccgatcgc
    17281 ggttgcgccc gcttcggtgg tcgcggtgcc gacgaccgtg gcacccgcct tccccaacgc
    17341 catggcaatc gccttgccga taccgcgcga tgcgccggtc accaatgcga cttgtccggt
    17401 cagcatgttc aagcctcctt cagcgatgcg atggcctgct cgatcgcggc ccggtcgttc
    17461 aacgccaagc cctgcacgcc gtcggcaatg cgcttgacca gcggcgcaag cactttgccc
    17521 gggccgcact cggcgacgtg cgtgacgcct tcggcagcca tggtgcgtac ggtttccacc
    17581 cagcgtacgg cgtggtcggc ctgctgcacc agcgcgttct tgatggcctc gccctcggga
    17641 tgacgctgca cgtcgaagtt gtgaatgacg ggaatgcgcg gcgtgctgat cgtcacattc
    17701 gccaggtatt cgcgcaggcg cgcggccgcc ggcgcgagca gcgatgaatg gaaaggcgcg
    17761 ctcaccggca gcgccacggc gcgcttggcg cccttctctc tggccaattc catggcgcga
    17821 tgcacggcgc cggcgtgccc cgcgatgacc acttgcgaag gcgcgttgaa attgaccgct
    17881 tcgacgactt cattctgcgc ggcggtgctg catacttcgc gcacggcgtc gtcttcgagc
    17941 cccaggatgg ccgccatcgc gcccgcgccc tgaggcaccg cctcctgcat gacctgcgcg
    18001 cggaagcgca ccagcggcaa cgcatcctcg aatttcagtg cgccggcgac cactagcgcg
    18061 gtgtactcgc ccagactgtg gccggcgacc acgctcggct ccggtccgcc cagcgattgc
    18121 cagacgcgat aggccgcgta gcccgccgtc accatgacgg gctgggtgtt gacggtgcga
    18181 ttcaactctt cggccgggcc ttcggcgatc agtttgccga tgtcctgttg caatacttcg
    18241 gaggcgatgt cgagcacttc gcgcacttcg ggcgcatcgc cgtaagcgcc gagcatgccc
    18301 acggactgcg agccctgccc gggaaaaacc attgccaact tcatgcttgc tccttgaacc
    18361 tcggttgcct gcgcaaccga ttacatctgc accagcacgg cgccccaagt gaaaccgccg
    18421 cccacgcctt cgagcagcac cttgtggccg gctttgatcc gtccgtcgcg cacggcgacg
    18481 tccaaggcaa gaggaatgga cgccgccgac gtattggcgt ggcgatccac agtgacgacg
    18541 acccgctcgg cggcgaaacc cagtcgccgg gcagtcgaat ccagaatgcg cagattggcc
    18601 tgatgcggaa tcagccagtc cacgtcgtcc agtcgcaggt tggcgcgatc caccgcttcg
    18661 cgtgccactt cgtcgagcac tttcacggcg aacttgaaga cggcctggcc gtccatttcg
    18721 acgaagggat tgccgacgat gcgcccgccg cacagcttgc ccggcgcggc gaggatgccg
    18781 ctgtagctgc cgtcggcatg catggcgctg gcaaggatgc cgggcttgtc gtcggcgcgc
    18841 aggacgaccg cgccggcgcc gtcgccgaac agcacgcagg tcgtgcggtc ctgccagtcg
    18901 agaatgcgag acagcgtctc ggcgccgacc accagcgccg tgcgcgcctg cccggcgcgg
    18961 atgaggctgt ccgctacggc cagcgcgtaa acgaagccgc tgcataccgc ctgcatgtcg
    19021 aaagccattg cgcccttgtt gccgaggcgc tgctgcagca ggcaggcggt gctgggaaac
    19081 acctggtcgg gggtggtcgt ggcgatgatg atgagatcga ggtcctcggc cctcacgccc
    19141 gcagtttcca gcgcctgctt gctcgcttcg agagcaagat cgctggtgtt ctggccctcc
    19201 tcggcgaggt ggcgttggcg gatacccgta cgcgtcgtga tccactcatc cgaggtgtcg
    19261 acggtctggg caaggtcgtg attggtgacg aggcgtgcgg gcagatagct gccggtgccg
    19321 ataatgcggg agtaggtcac gtggtctccg gaactgcgaa gcgctgcgtg atacgcgcca
    19381 gggcttcgtt gcgtacttcc tccgtcgcgc gttcgagcgc gcgggcgaat gcgaaagcgt
    19441 cggccgagcc gtggcttttc accacgatgc cgcgcaggcc gagcaaggag gcgccattgt
    19501 aacggcgatg atccacccgg ttgcggaatg cggacaacac cggccaggcg gcgagcgccg
    19561 ccaggcgcgt aagcgggttg cgggtgaatt cctcgcgcag gaaacgcgcg agcatttgtg
    19621 caagaccttc ggaggtcttg agtgcgacgt tgccgacaaa gccgtcgcac acgataacgt
    19681 cggcggcgcc tttgtagatg ccgtcgcctt cgacgttacc gatgaaattg agatcggagg
    19741 cgcgcaacag ctcggccgcc tgcttgacga cctcgttgcc cttgatggct tcctcgccga
    19801 tattgagcag gccgacggta ggacgcggct gttgctccac tgcggcgtag agcgaggcgc
    19861 ccatgatgcc gaactgcagc agatgctcgg catcgcaatc gacattggcg cccagatcga
    19921 gcatataagt cttgcccgtc atggtgggca gcacggatac gatggcgggg cggtcgatac
    19981 cgggcagggt cttcaggacg aaacgcgcga tggccatgag cgcaccggta ttgcccgcgc
    20041 tgacacaggc atccgcttcg ccgttcttga ccagattgat ggcgacgcga accgaggaat
    20101 ccttcttgtt gcgcagcgcg agggccggcg attcgtccat cgccacgact tcgcttgcac
    20161 gcacgacgcg cagacgagcg ctcgagacgg cacgatgcag cgcgagctgc gcctggattg
    20221 cgccttcctg accgaccagc gcgaccgaga cgcccgcatg tccggcaaga aattgcaacg
    20281 cggcggggac agtcacggcc gggccgtggt cgccgcccat gcaatcgatc gccagtctga
    20341 cgtccctgct catgactttt tatagtcgtg cccggccgag gccgggagcg cggctatgcc
    20401 gaggccctcc cctcactgtg tgccgcgtcg gcgttaatcg ttcttggtct ggacgacttt
    20461 cttgccgcgg tagtagccgc tcgggctgat gtggtgacgc agatgcgttt cgccggtgga
    20521 aggctcgacg gccagcggcg gattgctcaa cgaatcgtgc gagcgatgca tgccgcgctt
    20581 cgacggcgac ttcttgttct gctggacggc cattttggca gctccttcaa ctctctatta
    20641 cctgatctgt acacaaggac cgcctcatgt accgcttgtc gaacaacggc gccgggcgat
    20701 gacacacggc gccgcttttc aattcccggg atctcccgcc ttcttcttca atgacgcaag
    20761 cacggcaaac ggcgaaagct tgtccgcctt cgcctcgacc ggcgtcgcgc aatgcgcatg
    20821 catgggcacc atcggcaggt cgagaatcag ttcgtcctcg accagggaca ccacatccat
    20881 gctgtgcgtc gccagcaccc ggtccacgtc gtcgtccgca gtttcgatga aacccggatc
    20941 gaccgccagc tccagctcgc tgtgcacttc gactgcgtag tccagcggtt ccaggcagcg
    21001 ctggcattcc agttccagcc gaccgacgac atcgacgcgc agacaaagct gctcggccgc
    21061 gtttctcgcg ccctcgatca cgtagtcgac acgtactgca cgacagccca gttccgccag
    21121 acgcgacaac gcgtcgagca ccaactgccc cgagcaccgt ccgcactccc gcgcgaactc
    21181 caatccgtca atctgctcgg cttgcgacat aatcgcggga tgatattatt tttgcctctc
    21241 agtcgtcaaa aaaatcatag
//
LOCUS       DATGOU010000079        23785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_1994559, whole genome shotgun sequence.
ACCESSION   DATGOU010000079 DATGOU010000000
VERSION     DATGOU010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 23785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23785
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1994559"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="VKZ48_04815"
     CDS             complement(<1..140)
                     /locus_tag="VKZ48_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015420459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HLU77208.1"
                     /translation="MRCPFCKSGDTAVLDTRLSDDGGTVRRRRECDDCHKRFTTYERV
                     KL"
     gene            complement(160..1443)
                     /gene="glyA"
                     /locus_tag="VKZ48_04820"
     CDS             complement(160..1443)
                     /gene="glyA"
                     /locus_tag="VKZ48_04820"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HLU77209.1"
                     /translation="MYNDQTLAQADPELWAAIDAEYRRQEDHIELIASENYASPAVLE
                     AQGSVLTNKYAEGYPGRRYYGGCEHVDVVEQLAIDRAKALFGAEYVNVQPHSGSQANQ
                     AVYAAVLKPGDKILGLSLAHGGHLTHGAKVSSSGKLYQSFEYGLNDDEEIDYDQVEHL
                     ARGHKPKMIVAGASAYSLVFDWKRFRKIADSIGAYLMTDIAHYAGLIAAGVYPSPVGI
                     ADFVTTTTHKTLRGPRGGMIMAGKEHEKILNSMVFPYLQGGPLEHVIAAKAVAFKEAA
                     TKEFKRYQEDVVNNARVMAKVLSERGLRIVSGRTDCHLFLVDLRAKNITGKEAEEALG
                     RAHMTVNKNAIPNDPEKPMVTSGIRVGTPALTTRGFEEFEAETVANLIADVLENPRDE
                     ATIQRVAEEVKALCKKFPVYPEGMRAARPAQRGSA"
     gene            complement(1516..2397)
                     /locus_tag="VKZ48_04825"
     CDS             complement(1516..2397)
                     /locus_tag="VKZ48_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HLU77210.1"
                     /translation="MRIGIDLGGTKIEIIALDDAGRTRLRRRVATPRGDYDATLRTVV
                     QLVRDAEAEVGVRARVGIGTPGSASPANGLMRNANSVCLNGRALRDDLERLLGRDIRM
                     ANDANCFALSEAMDGAGAGAHCVFGVILGTGVGGGLVVDGRVLEGSNGIAGEWGHNPL
                     CGETPEQAPACCCGRRGCIETWLSGPGLLADHARQGGREPDVPAIVEGAVRGDPQCEA
                     TLARYERRLARALAVVINIVDPEVIVLGGGLSNIARLYERVPQLWREHIFSDVVRTRL
                     LPNRHGDSSGVRGAAWL"
     gene            2493..2735
                     /locus_tag="VKZ48_04830"
     CDS             2493..2735
                     /locus_tag="VKZ48_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77211.1"
                     /translation="MRDEPGNIPALHGAYDAQPAQAQWTPTLRTAAGAAGASLLLGGL
                     LRRNSAGIALGALVAGLIAVGATGKTRRKRAPQPAL"
     gene            2876..3619
                     /locus_tag="VKZ48_04835"
     CDS             2876..3619
                     /locus_tag="VKZ48_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HLU77212.1"
                     /translation="MPLSVDAITAQHIGDRQEQQDRVALLSSRRVKGAALAVLADGAG
                     GHKGGAGAAEQVIRTAANLFEGWSPKSESAQTLLRAIVDESHTVIRLNRVLTEEEPHS
                     TVVLMLVQPDGVDWAYVGDSRLYHYNGPQLVARTRDHSLVEVQIQSGHWPEADRHRHP
                     HRNLLLQALGHSETPEPEFGHAPAPRAGDSFLLCSDGLWDYITDVELGKVLDQLPPRH
                     AAETLLNAARQRANGRGDNVSLAIVKFGE"
     gene            complement(3675..5507)
                     /locus_tag="VKZ48_04840"
     CDS             complement(3675..5507)
                     /locus_tag="VKZ48_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HLU77213.1"
                     /translation="MSTLPRGAAAIRYRLVPARPEAHLYEVSCTVLRPDPEGQRFALP
                     VWIPGSYLVRDFARHVVSIRARSGRREVPLIKLDNNTWQAAPCDGVLTVTMEVYAWDL
                     SVRGAHLDTTHGFFNGTSVFLRVVGQESQRCEVELVRPRGRRYAAWRVATAMRRKQAR
                     AYDFGLYQAADYDELIDHPVEMGTFTLATFKARGVPHDIAITGRHEADMDRLCRDLKR
                     LCEWQIDFWGVAPMDRYVFLVTAVGEGYGGLEHRASTALLCSRDDLPRAGLRGTDEAY
                     RNFLGLCSHEYFHTWNVKRLKPAAFTPYDLDRENHTTLLWAFEGITSYYDDLALVRCG
                     LITPAQYLETIGRTATQLLRNGGRHKQTISESSWDAWTKFYRPDENTPNAVVSYYGKG
                     SLVALCLDSLIRERTRGRKSLDDVMRALWQRYGETGEGVPEDGVERMAEEVAGTRMRA
                     FFDRALRSTAELPLRERFAALGVDMALRPAESSADRGGKPSTKSAKALAACGSLGAKT
                     ANAGADVKLAHVYDGGAAQAAGLSAGDVLVAIDGLRVTGKNVEKMLARRQPGTVLNVH
                     AFRRDELHSFKVTLQAPPADTCVLQFAPRRNVSQRNWVDDRAEA"
     gene            complement(5545..6744)
                     /locus_tag="VKZ48_04845"
     CDS             complement(5545..6744)
                     /locus_tag="VKZ48_04845"
                     /inference="COORDINATES: protein motif:HMM:NF014324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HLU77214.1"
                     /translation="MHRSLLLFVGGALLLLSTQASAAWLLNRVSESTVPGEPVELELI
                     VLNDTTEPIEGNVPPRIPAVLTTRDGAVDVELTVVDAKSAPEAQLWPGQFRKHRLRLL
                     LPTGVHGTIALTVAPAVRLVMVTAPPTTVAAAPEARSAPAEVRVQEPDPVLRATDTTP
                     PPALQTHEPMYFLVGRREGMTSARFQLSFKYRFFDERNPLRRIAPVDKLYFGYTQTSI
                     WDLSEDSSPFRDTVYRPSLFYFDPDVWSSTDGSHTIGLSAGLEHASNGRGGDDSRSIN
                     IAFVEPSWRKFLSDSKWYVSVSPKLWAYLDKEDNPDIADYYGYGALSLKLGRVDGWLF
                     GARWRKGTDTMGAVQLDASYPIRRPFFANAGGYVHFQYFNGYGESLIDYNNKRASQFR
                     VGVSIVR"
     gene            6845..7420
                     /locus_tag="VKZ48_04850"
     CDS             6845..7420
                     /locus_tag="VKZ48_04850"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HLU77215.1"
                     /translation="MEIHGVQRIRLPQATVWGALNDAQIIRQSIPGCRRFRQLTDDRY
                     DVMVLTAVGPVSTEFEGTVSLSRLQPPASYTIAFEGEGQGAGTVLGQADVQLVPEDLG
                     TTLTYRADMQVTGPLAQAGPRLLEEAARRMVEGFFARFNAAVEPGAPGIAAGEARVTE
                     TPGGLWLTPLWAFLGGFGAGLVAIIAATILQ"
     gene            complement(7426..8067)
                     /locus_tag="VKZ48_04855"
     CDS             complement(7426..8067)
                     /locus_tag="VKZ48_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77216.1"
                     /translation="MFVRVLTLCLLLVGTGAIAARNVTLTAHAASIIQELPYQEMLVG
                     QQPTLTRAGFELTPVARFEVTGRVLGRLVYESDTVAELSPVDIAIGWGPMSDRRVLAQ
                     IEMSQGERFFYWQTARAPLPRTAIEQSSTNVHTIPATPEIERKLRMVQPDDVVHLAGV
                     LVNAYRPTDGYRWTTSLVRDDTGDGACEIVYVTDIEIAPAQPRGTYVQHASAE"
     gene            complement(8397..9485)
                     /locus_tag="VKZ48_04860"
     CDS             complement(8397..9485)
                     /locus_tag="VKZ48_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HLU77217.1"
                     /translation="MQRRSFLKRAGAGLALAAPAAAYAQAQPELHWRLASSFPKSLDA
                     LYGAGEHFARRVAQLTDGRFQIRVFAAGEIVPPLQVLDAVQSGTIECGHTASYYYVGK
                     HPGFAFDTTLPFGLNARQQNAWMYEGGGIALLRELFGRYGCVQFPAGNTGAQMGGWFR
                     KEVNTLADLQGLKFRIAGIAGQVMARLGVVPQQIAGSEIYQALERGAIDAAEWVGPYD
                     DAKLGFVKVAPYYYYPGFWEGSAQLSVLVNAAHWQGLPPAYQAAVEVAANETNTVMTA
                     QYDTRNPVALRELVRSGARMRAFPKPVLEAAYRAAFDLYDELARQDPLFDKIYRSWRG
                     YRDESHLWFRVAEQTFDSFVYAQARKRV"
     gene            complement(9512..10852)
                     /gene="pmbA"
                     /locus_tag="VKZ48_04865"
     CDS             complement(9512..10852)
                     /gene="pmbA"
                     /locus_tag="VKZ48_04865"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HLU77218.1"
                     /translation="MPNERFAYTLDAFRNIIDDVLRRAKERGASACEADVSEGYGQSV
                     TVRCGEVETIEYNHDKGLGVTVYIGQQKGYASTTDLSPKAIEDTIDAALSIARFTAAD
                     EAAGPADPELIARESRDLDLFHPWDISVEQAIETARACEAAAFRLDPRVVNSEGATLS
                     AQQSQFISGNSAGFLDGFRSSRHYIACAVIAAEGEQMQRDDWYSSERRPADLASPEAI
                     GDYAGRRALARLRSRKISTREARVLYEAPVANGLLAHFVGAASGGSLYRKSSFLLDSL
                     GKPVFSPLVQLREEPHLPRAHASSYFDDDGVATHPRDVVKDGVLQGYFLSVYSARKLG
                     MQTTGNAGGNHNLILAPGTLDFEGMLKELGTGLLVTELMGQGINLVTGDYSRGAAGYW
                     VENGEIQYPVEEITIAGNLRDMYRNIVAVGGDTLVRGSRRCGSILIDGMTIAGA"
     gene            10923..11429
                     /gene="yjgA"
                     /locus_tag="VKZ48_04870"
     CDS             10923..11429
                     /gene="yjgA"
                     /locus_tag="VKZ48_04870"
                     /inference="COORDINATES: protein motif:HMM:NF016626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HLU77219.1"
                     /translation="MFDDEKPSKTRRKKDMHALQALGEALVELRPEQLEGVDMPEALR
                     DAVLEAKRLTRHEARRRQMQYIGKLMREVDAAPIRAKLDELAGRSATATAALHRVERW
                     RDRLIEDEQAFNEFAQTYPHADLQPVRTAVREVRKERAASRPPRHYRELFRLLKAIDE
                     SRGHADAD"
     gene            11413..11853
                     /locus_tag="VKZ48_04875"
     CDS             11413..11853
                     /locus_tag="VKZ48_04875"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HLU77220.1"
                     /translation="MRMRIEGSRTALALMFAAGTAVAQTDALQPGLYEVELRTQFSDA
                     QGESVVPVSVVRRCVSATEIQSPERLAPNFSAAAACVWSAHRPEPGGASWTVDCQGDP
                     VMRGEAHVRWTKDRYAGETRMVLRRGAERMNMTQQYTAHRLGGC"
     gene            11867..12466
                     /gene="mog"
                     /locus_tag="VKZ48_04880"
     CDS             11867..12466
                     /gene="mog"
                     /locus_tag="VKZ48_04880"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013213092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HLU77221.1"
                     /translation="MNMLDRPLKIGLVSISDRAATGVYQDEGLPALKEWFKTALATPW
                     ETVERLIPDEQSEIERTLIDLVDNEACDLVLTTGGTGPAPRDVTPEATLAVADKVMPG
                     FGEQMRQVSLKFVPTAILSRQVGVVRKKALILNLPGQPKAIRETLEGARDADGNLLVN
                     GIFAAVPYCIDLIGGPYVETNENVVKAFRPKSAQRARQP"
     gene            12479..13144
                     /locus_tag="VKZ48_04885"
     CDS             12479..13144
                     /locus_tag="VKZ48_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLU77222.1"
                     /translation="MSELLPAVEIETAPNPRTSIIWMHGLGADGNDFVPIVNELDLPD
                     TPIRFVFPHAPVMPVTINYGHRMRAWYDVLSSDFAERTDNEGVRASQKLVEALIAREK
                     ERGSAADRIVIAGFSQGGAMALHTGLRHAERLAGIMALSTYLPIPDALTQEGSAANRD
                     VPIFMAHGDQDQVIPIEYAAQSRDLLTTQGYQVEWHAYHMPHSVCMEEIAHIGAWLQR
                     VLG"
     gene            13144..13818
                     /locus_tag="VKZ48_04890"
     CDS             13144..13818
                     /locus_tag="VKZ48_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77223.1"
                     /translation="MRKVTLTALAGALVLAAIAGYLYMQREQLLQRAIEHYGSAILGA
                     SVSVSSVHLSPADGDGAMRGLRIGAPLGFARDVARAEAIELSVDPATLADPVVHIRRI
                     VLSGPDIAYEQGRSGTNLEALQRNVRRYIGEASAESRSAKRFIVDRLVIRDAQLTYIP
                     LSGVSSAAATMRLPDITLRDIGKRRGGVTAGELSGIIVDALIARTAGAVGQSVLDRTV
                     NRLLRR"
     gene            complement(13796..15340)
                     /locus_tag="VKZ48_04895"
     CDS             complement(13796..15340)
                     /locus_tag="VKZ48_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77224.1"
                     /translation="MLNIFNRKSSDHPFGDLKGARELLGGIAEGEAQAAIDDLTHWLQ
                     SVAAEADFRPDHRSQALLLIDEAAQPHLRKLSRDYLGSTRLLKQQEMRIWNHVDAYLQ
                     EAARAFAAVLEQMVALRPGDAARATTALVAVRALRSLTGQLKWMHLRYGPVSDGLWST
                     VSRVYAASEQARVSQMSVVVYPNVPGESSMQQELLRLFMFAACSPGALAPVDMELADR
                     LIAQFGPKFAAAAEAGPETPYWVDLGAAAAPRRGPVSGGAKGPVGYFGAGAAHEDLLA
                     LAAEIRASGAVPSAVNLGGTYETERILAVIDHLEQHWSPKLPERKSIRQAAKLRLTVA
                     RGFDGVLDVLQLPPGAAPAGEEIESWIMEDMSAGGFGARVPAMKDEWLHIGCLLGLHY
                     EGGAHWSAGIVRRLARSTAQQTNVGIQLLSRVAMPVELRIKTEYGLSLDSEVGILLPA
                     PRREEETRLLLRPGVYAPGQLYRIETAAGERLFAPTRLMERGSDYELIACRETQVIDF
                     TASING"
     gene            complement(15517..15744)
                     /locus_tag="VKZ48_04900"
     CDS             complement(15517..15744)
                     /locus_tag="VKZ48_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77225.1"
                     /translation="MRAHRKHLSSTEQRLQAEEARMEQDFGAPNPHESHALAEKFVAR
                     LKEELELVERRREAMALDEAQTILRRIAAKR"
     gene            complement(15932..16007)
                     /locus_tag="VKZ48_04905"
     tRNA            complement(15932..16007)
                     /locus_tag="VKZ48_04905"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15972..15974),aa:Thr,seq:cgt)
     gene            16287..16727
                     /locus_tag="VKZ48_04910"
     CDS             16287..16727
                     /locus_tag="VKZ48_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HLU77226.1"
                     /translation="MRPQRGFTLIEAMITVAVIAILSMIAMPAYTDYVLRGKISEAVQ
                     GLADARVKQEQFFLDNRTYGPAATTCGAPLPQNTKHWDFSCSNVSTTTYTVTATGKAS
                     GGTAGMSYSIDEENVRSSVINASVGPKGWTGQANCWVVRKGGVC"
     gene            16721..17308
                     /locus_tag="VKZ48_04915"
     CDS             16721..17308
                     /locus_tag="VKZ48_04915"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HLU77227.1"
                     /translation="MLMHGRGFTLIELMIGIALMALLLTFGMPSFSTMMHNMKVRSMA
                     ESLVHGLQNAKAEAIKRNQPVEFILTTDETDPDDVASYTKSTNGRNWAIRISDGGGYT
                     FVEAEAGNEAANNPVQVAATGGGFDGIVTFSGLGTTTLASDVALDITNPIAGACKTAD
                     GDEPVRCLRVVVTTSGRVRMCDPSVNAAGGDTRAC"
     gene            17348..17821
                     /gene="pilV"
                     /locus_tag="VKZ48_04920"
     CDS             17348..17821
                     /gene="pilV"
                     /locus_tag="VKZ48_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HLU77228.1"
                     /translation="MLIEALIGILIFSIGILAMIALQAASISATADAQYRVEAVNLAN
                     QMMGQIWAGVDRSTSAALENSLLTFEHLADEGDDNCNFEGTESTNTMVTRWRNLIVTG
                     AADGTGTRPLLPGASNTDQQIDVNIGANNQVKITVCWRTPPDNVTHRHVLLANVN"
     gene            17831..18982
                     /locus_tag="VKZ48_04925"
     CDS             17831..18982
                     /locus_tag="VKZ48_04925"
                     /inference="COORDINATES: protein motif:HMM:NF027400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HLU77229.1"
                     /translation="MSTLLSKTGRHGTPNERGMSLVEILVGILIGMIAIIVIFQVLSV
                     YEDRKRTAVGAGDAQTAGSIALYMLDRDLRLAGYGYSDAETMGCNVVGHDATTGAALN
                     FTLAPAIISKPNGAAGPDAIISLRGSSPLFVARKAFTSSADTEKIMQAGHRPGILRGD
                     ILVVSPPTPGPCALVEVTDNTAGDKVTILHETGSYTSDYSAGAVAARYNKDGGLGVAF
                     TQGFLYSLGPSPTRTVWSVTGNRLSYTDTLHADQGAIDVADGVVNLKAEYGIDANDNN
                     ELEQDEWTTTDPTTAAGWRAIRAIRVAMLMRSSQWDPNVCSTNPQWTSGASGTPTLTN
                     FVMRNVDGTNDSYSDCNGTNADPNNWRTYRYRVYETVIPLRNMIWGTAP"
     gene            18979..19560
                     /locus_tag="VKZ48_04930"
     CDS             18979..19560
                     /locus_tag="VKZ48_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77230.1"
                     /translation="MIKQGHQPHSLKRRQRGVVLFIALIVLVALTLAGLAMVRSSTSG
                     ILVAGNLAFKQTATASSDAGVEAARAWLRTKGPAELEASDATQGYYATWEPTFDPATS
                     AIWEPNTSRSVGAADDAGNTVRYVIHRMCDAAGALKDVDCVTVASGGLGGSKGGGCYG
                     CKAFTGSSQPYFRITVRTTGPKNTVSYVQAMMY"
     gene            19579..23427
                     /locus_tag="VKZ48_04935"
     CDS             19579..23427
                     /locus_tag="VKZ48_04935"
                     /inference="COORDINATES: protein motif:HMM:NF017388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HLU77231.1"
                     /translation="MARKNLNSHARKDRLAQLSLAIALCFCLPAGGAELTQLADAPLS
                     SSAAEQVPPNIMFILDDSGSMAWTHLPDISNSSTIENNTVGYRNHLCNPMYFNPDEVY
                     KPPVDKDGKAYDDMDFDKALYNGFIPTDKSPDTGTSKINLGSQFRAYDTKTTANMSGG
                     SVRASAEAAHYYKWKGAGTPSPTECDKRISNPGSNWEKVTAADFTDDDKTNFANWYSY
                     YRTRMLLMKTAAGRAFSILDKHYRVGFLTINPMKSSGRSGQTIDEKKYLKISEFESDH
                     KEDWYDIFYSQIASGGTPLREALSRAGRHFAGKSDGINNGMTDDPLLYSCQPNFAILT
                     TDGTWNGNPGQTINGGSIGNQDGDLAFTPRPMYDGGEVEKKDITTYTEWRYRSRNSGK
                     PKCSEDEQSATKETRTITVTTTTTQYGTSSTKPTTGSTKSQNICLPNGVDRTTDWAIE
                     SGSETNETITAGKGSASNTLADVAEYYYRTDLRSDTATRTGPNGEQINIGADNVPVAG
                     TGPEDDKATHQHMTTFTVGLGVSGRLNFDPNYKSAKTGDFQDIRDGKKGWPDPSSSDL
                     AKTDDLWHAAVNGRGQAFSAQDPYTLATSLQGALSNIQARIASSAAAATSNLEPTETD
                     RLVFLPKYVSAKWTGEIEAHLLNLTTGEVEDDLTWSAQAGLDGQTQADCDDRNIYLYR
                     KGATNNLVDFAWDTKACEDNSITTGLDPDEKAFFSSNHINGLSQWLTMSDGTDGTANQ
                     KAAAADANLVNFVRGQRGKEGYMPNDADKLYREREHVLGDIVGAQPVYVAAPAFAYTE
                     NGYPTFKTNNASRAGMVYAASNGGMLHAFKVGTLANNFADGGKEAWAFIPRTVLPNLY
                     KLADTNWASKHEFLVDGSPVVGDIYDTSASAWKTILVGGLNKGGKAYYALDITNPNSP
                     KALWEFAHSDTCYDKDAPATHYADCHIGYTFGNPIITKIAGKDASNNEWQKWVVLVTS
                     GYNNVNEPAKAGDGVGYLYVLDAVSGRILHKVSTGYGDSGAGDNSVGPSGLAKINNWV
                     VGDAYFDAKSSAVYGGDLLGNIYRFNVDVEPPTVKLIGTAKDDSGAGQPITTKPELAE
                     VGTPPSPYVYVATGRYLASPDVSNTQTQSLYAIKDPGAAAGYTNLRDSLLRLEMTNVN
                     ADNERFVNCFENCSSTNGWTVDFPDSGERVNVDMKLQLGTLMVLSNVPKNNTCEPGGY
                     SYANYFHYASGQSPLGIGQPIGGKLSGSLAVGFNVIRLPSGKVIVISTDAAGNTSASE
                     APIGSGAPTGKRVSWRELLN"
     gene            complement(23511..>23785)
                     /gene="ispH"
                     /locus_tag="VKZ48_04940"
                     /gene_synonym="lytB"
     CDS             complement(23511..>23785)
                     /gene="ispH"
                     /locus_tag="VKZ48_04940"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003572.1"
                     /note="catalyzes the conversion of
                     1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into
                     isopentenyl diphosphate (IPP) and dimethylallyl
                     diphosphate (DMAPP); functions in the nonmevalonate
                     isoprenoid biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HLU77232.1"
                     /translation="STNSSNSNRLREVAQNMGVPAYMVDHEDQIDPQWVSGKTRVGVT
                     AGASAPEVLVNRVLERLKQLGAQRVQQLDGAAENVSFPLPKGLSLG"
BASE COUNT         4611 a         7610 c         7441 g         4123 t
ORIGIN      
        1 tgcagtttga cgcgttcgta ggtggtgaag cgcttgtgac agtcgtcgca ttcgcgccga
       61 cggcggacgg tgccgccgtc atcggacagg cgcgtatcga ggaccgccgt atcgcccgac
      121 ttgcagaacg gacatctcac ggacggacct cgtcttcgtt caggcgctgc cgcgctgcgc
      181 cggacgggcg gcgcgcatcc cttcgggata gaccgggaac ttcttgcaca gcgccttcac
      241 ctcttcggcc acgcgctgga tggtcgcctc gtcgcgcgga ttctccagca cgtcggcgat
      301 gaggttcgcc accgtctcgg cttcgaactc ctcgaagccg cgcgtggtga gcgccggcgt
      361 gccgacccgg atgccgctgg tgaccatcgg tttttccgga tcgttcggaa tcgcattctt
      421 gttcaccgtc atgtgcgcac ggccgagcgc ttcttccgct tccttgccgg taatgttctt
      481 cgcccgcaga tcgaccagga acagatggca gtcggtacgg ccggaaacga tgcgcaggcc
      541 gcgctcgctc agcaccttgg ccatcacgcg cgcgttgttc accacgtctt cctgatagcg
      601 cttgaactcc ttcgtcgccg cctccttgaa cgcgacggcc ttggcagcga tgacgtgctc
      661 cagcggtccg ccctgcagat aggggaagac catggagttg aggatcttct cgtgctcctt
      721 gccggccata atcatgccgc cgcgcggacc gcgcagagtc ttgtgcgtag tagtggtgac
      781 gaaatcggcg atgccgaccg ggctcggata gacgcccgcc gcgatgagac cggcgtaatg
      841 cgcgatgtcg gtcatcaggt aggcgccgat gctatcggcg atcttgcgga agcgcttcca
      901 gtcgaacacc agcgaatacg cggacgcgcc ggcgacgatc attttcggct tgtggccgcg
      961 tgccagatgc tcgacctggt cgtagtcgat ctcttcgtcg tcgttgagac cgtattcgaa
     1021 ggactggtag agcttgccgg aggaactgac tttcgccccg tgggtcaggt gaccgccgtg
     1081 cgcgagcgac aggccgagga tcttgtcgcc cggcttgagc accgcagcgt acacggcctg
     1141 gttggcctgc gagccggaat gcggctgcac gttgacgtat tcggcgccga acagggcctt
     1201 ggcacgatcg atcgcgagtt gctcgaccac gtcgacatgt tcgcagccgc cgtagtagcg
     1261 ccggcccgga tagccttcgg cgtatttgtt ggtcagcacc gaaccttgcg cctcgagcac
     1321 cgcgggactg gcgtagttct cggacgcgat gagttcgatg tggtcttcct ggcgccggta
     1381 ttcggcgtca atggcggccc acagctcggg gtccgcctgt gcgagagtct ggtcgttgta
     1441 catgttcgta cagagaaaaa aggcgttgcg agtgtagcac gcggccccct ttttatcccc
     1501 cggccgcgcc cggcatcaga gccacgcggc gccgcgcacg ccgctggaat cgccgtggcg
     1561 gttggggagc agccgggttc gcaccacgtc cgagaaaatg tgctcccgcc acagttgcgg
     1621 gacacgctcg tacagccgcg cgatgttgga caggcctccg cccagcacga tgacctcggg
     1681 atcgacgata ttgatcacca cggccagcgc ccgcgccagg cggcgttcgt agcgggcgag
     1741 cgtcgcctcg cactgcggat cgccgcgcac ggcgccctcc acgatagccg gcacatccgg
     1801 ctcgcggccg ccctgccgcg cgtgatcggc cagcagaccg gggccggaca gccaggtctc
     1861 gatacagccg cgccggccgc aacagcaggc tggcgcctgt tccggcgtct cgccgcacag
     1921 cggattgtgt ccccactcgc cggcgatgcc gttgctgccc tccagcacgc gcccatcgac
     1981 gaccaacccg ccgcccacgc ccgtgccgag gatgacgccg aacacgcagt gcgcgccggc
     2041 gccagcgccg tccatcgcct ccgacagcgc gaagcagttc gcatcgttcg ccatacggat
     2101 atcgcgcccc agcaggcgtt ccaggtcgtc gcgtagcgca cgaccgttca ggcagacgct
     2161 gttggcgttg cgcatcaacc cgttcgccgg cgacgccgag ccgggcgtgc cgatgcccac
     2221 gcgtgctcgt acgcccactt ccgcttcggc atcgcgcacc aattgcacga cggtacggag
     2281 cgtcgcgtcg tagtcgccgc gcggcgttgc cacgcggcga cgcaaacgcg tccggcccgc
     2341 gtcgtcgagg gcgatgattt cgattttggt gccgccgaga tcgatcccga tgcgcatgcg
     2401 cccctcctct ttcgcgccga agcttagcgc ggatgtgggg cgtgtgattt gcgaaatccg
     2461 ccgcaccggc gcccgcacac cacgtccgcg atgtgcgcga cgagccgggc aatatccccg
     2521 ccttgcacgg cgcttacgac gcgcagccgg cccaggcgca atggacgccg accctacgga
     2581 cggcggccgg tgcagcgggc gcctcgctgc tgctcggcgg cctgctgcgc cgcaacagtg
     2641 cgggtatcgc gctcggtgcg ctggtcgccg gactgatcgc ggtcggcgcg acgggcaaga
     2701 cgcgccgcaa gcgggcgccc cagcccgcgc tctaagggtt ttggacgaac ttcgaggctc
     2761 gcttggcgcg cagcgtgaca taccatgcgc cgcgcgcacg gctgcggatg cgcaaacgcc
     2821 gcagttgagg tagcttgcgg acggttatca attcgcatta cccgcccgct tctccatgcc
     2881 ccttagcgtc gacgcgatca ccgcgcagca tatcggtgac cgacaagaac aacaggatcg
     2941 cgttgcgctg ctctccagcc gccgcgtgaa gggtgccgca ctcgcggtcc tggccgacgg
     3001 cgcgggcggg cacaagggcg gtgccggcgc cgccgaacag gtgatccgca ccgcggccaa
     3061 tctgttcgaa ggctggtcgc ccaaaagcga aagcgcacag accctgctgc gcgccatcgt
     3121 cgacgaatcg cacaccgtca tccgcctgaa ccgcgtgctc accgaagagg aaccgcacag
     3181 cacggtggtg ctcatgctgg tgcaacccga cggcgtcgat tgggcctacg tcggcgacag
     3241 ccggctatat cactacaacg ggccgcaact ggtcgcgcgc acacgcgacc attcgctggt
     3301 ggaagtgcaa atccagtcgg gccactggcc ggaagcggac cgccatcgcc atccgcatcg
     3361 caatctgctg ctgcaggcgc tcgggcattc ggaaacgccg gagccggagt tcggtcacgc
     3421 gccggcgccg cgtgcgggcg acagcttcct gctgtgctcg gacggtctgt gggactacat
     3481 cacggacgtc gaactgggca aagtgctgga tcaactgccg ccgcggcacg ccgccgagac
     3541 gctgctcaat gcggcgcggc agcgcgccaa cggtcgcggc gacaacgtct cgctggcaat
     3601 cgtgaagttc ggcgagtaaa tcgagtcaag agccgagaat cgagagcgcc ccgacgcaag
     3661 caacggcggc cgcgctatgc ctctgcccgg tcgtccaccc agttgcgctg cgagacgttg
     3721 cgccgcggcg cgaactgcag aacgcaggta tccgccggcg gcgcctgcag ggtgactttg
     3781 aagctgtgca gttcgtcgcg ccggaaggca tgcacattca gcaccgttcc aggttgacgg
     3841 cgcgccagca tcttctcgac attcttgccc gtcacccgca ggccgtcgat ggcgaccagc
     3901 acgtcgccgg cagagaggcc cgccgcctgt gccgcgccgc cgtcgtacac atgcgcaagt
     3961 ttcacatcgg ctccggcgtt cgcggtcttc gcgcccagcg agccgcacgc ggccaatgct
     4021 ttcgcggact tcgtactcgg cttgccgccg cgatccgcgc tcgattcggc cggacgcaaa
     4081 gccatgtcca cgcccaatgc cgcaaaacgt tcgcgcagcg gcagttcggc ggtggaacgc
     4141 aatgcgcggt cgaagaaggc gcgcatccgc gtgcctgcca cctcttccgc catacgctcc
     4201 acgccgtcct cgggcacccc ttcaccggtc tcgccgtagc gctgccacag cgcgcgcatc
     4261 acgtcgtcga gcgatttgcg accgcgcgtg cgttcgcgga tcagcgaatc caggcatagc
     4321 gcgaccaggc tgcccttgcc gtagtagctg accaccgcat tgggcgtgtt ctcgtcgggg
     4381 cgatagaact tggtccaggc gtcccagctc gattcgctga tggtctgttt gtggcggccg
     4441 ccgttgcgca gcagttgcgt cgcagtgcga ccgatcgtct ccagatactg cgccggcgtg
     4501 attaggccgc agcgcaccaa cgccagatcg tcgtagtagg acgtgatgcc ttcgaaggcc
     4561 cacaggagcg tggtgtggtt ttcgcgatcc aggtcgtagg gcgtgaaagc cgcgggcttg
     4621 aggcgcttga cgttccaggt gtggaaatac tcgtggctgc acaggccgag gaagttgcgg
     4681 taggcctcgt cggtgccgcg caatccggcg cgcggcagat cgtcccggct gcatagcagc
     4741 gcggtggagg cgcgatgttc cagaccgccg tagccctcgc ccaccgcggt gacgaggaac
     4801 acgtagcgat ccatcggcgc gacgccccag aaatcgatct gccactcgca cagccgcttc
     4861 aggtcgcggc acaagcggtc catgtcggct tcatgccgac cggtgatggc gatatcgtgc
     4921 ggcacgccgc gcgccttgaa ggtcgcaagc gtaaatgttc ccatctccac cggatggtcg
     4981 atgagttcat cgtaatctgc cgcctgatag agtccgaaat cataggcgcg cgcctgcttg
     5041 cgccgcatgg cggtggcgac gcgccacgcc gcatagcgac ggccgcgcgg ccgtacgagt
     5101 tccacctcgc agcgctgcga ttcctggccg acgacgcgca gaaaaaccga cgtgccgttg
     5161 aagaagccgt gcgtggtgtc cagatgcgcg ccgcgcacgg acagatccca cgcgtagact
     5221 tccatggtga cggtcagcac gccgtcgcac ggcgccgcct gccaggtgtt gttgtcgagt
     5281 ttgatcagcg gcacttcgcg ccggccggag cgtgcgcgga tactcaccac atgccgcgcg
     5341 aaatcgcgca cgagatagct gccgggaatc cacaccggca gcgcgaaacg ctgtccttcc
     5401 ggatcgggac gcaataccgt gcaactgact tcataaagat gggcttcggg ccgcgcgggc
     5461 acgagacgat aacggatggc ggctgcgccg cggggaagtg tgctcatggg atctcttgta
     5521 cttgcttgat ggtcaggcgc gcgcttagcg cacgatcgac acgccaacgc ggaattgcga
     5581 tgcgcgcttg ttgttgtagt cgatcaggct ttcgccgtaa ccgttgaaat actggaagtg
     5641 tacatagccg ccggcgttgg cgaaaaacgg ccgccggatc ggataggacg cgtccagttg
     5701 caccgcaccc atggtatccg tccctttgcg ccatctggcg ccgaacagcc agccgtccac
     5761 gcggccgagt ttgagactca gcgcgccgta accgtagtag tccgcgatgt cggggttgtc
     5821 ctccttgtcc agatacgccc acagcttggg cgagacggag acgtaccact tgctgtccga
     5881 caaaaacttg cgccaggacg gttcgacgaa cgcgatattg atcgagcgcg aatcgtcgcc
     5941 gccgcggccg ttggaggcgt gttccagacc cgcggagagg ccgatcgtat ggctgccgtc
     6001 ggtgctgctc cacacatcgg gatcgaaata gaacaggctg ggccgataga cggtatcgcg
     6061 gaagggcgag gagtcctccg acaaatccca gatggaggtc tgggtgtagc cgaaatatag
     6121 cttgtccacg ggcgcgatcc ggcggagcgg gttgcgctcg tcgaagaagc ggtacttgaa
     6181 cgacaactgg aagcgtgcgc tggtcatgcc ctcgcgtcgt ccgaccagga aatacatcgg
     6241 ctcatgcgtc tgcagcgcgg gcggcggcgt cgtgtcggtg gcgcgcagca ccggatccgg
     6301 ttcctgcacg cgaacttcgg cgggtgcgga gcgcgcttcc ggcgcggccg ccacggtggt
     6361 gggcggcgcg gtcaccatca ccagtcgcac agcgggcgcc acggtgagcg cgatcgtccc
     6421 gtgcacgccc gttggcagaa gcagacgcag gcgatgcttg cggaactgac cgggccacag
     6481 ttgcgcctcc ggcgcggatt tggcatcgac caccgtcagt tcgacgtcca ccgcgccgtc
     6541 gcgcgtggtg agcacggccg gtatgcgcgg cggcacgttg ccctcgatcg gttccgtcgt
     6601 gtcgttgagg acgatcagtt ccagttcgac cggttcgccc ggaaccgtgc tttcgctgac
     6661 gcggttcaac agccacgcag ccgatgcctg cgtggaaagc agcagcaaag cgccgccgac
     6721 aaacagtagt agagaacgat gcatcgtgat gtagagagga atgaacgtat tgatttcacc
     6781 atatcgcgga gccggtgcga cgcaatcggg tccgcgccgg cacgcgcgtt gcacttgccc
     6841 cgacatggaa atccacggcg tacaacgtat tcgcctgccg caggccacgg tgtggggtgc
     6901 gctcaacgat gcgcagatca tccgccagtc tatcccgggt tgccggcgct tccggcaact
     6961 gacggacgac cgttatgacg tgatggtgct gactgcagtc gggccggtct ccaccgaatt
     7021 cgaaggcacg gtgagcctgt ccagactgca gccgcccgct tcttacacca tcgccttcga
     7081 aggcgaggga cagggcgccg gcacggtgct gggacaggcc gatgtgcaac tggtaccgga
     7141 agatctgggc acgacgctga cgtatcgcgc cgatatgcaa gtgaccggcc cgctggcgca
     7201 ggccggaccg cgcctgctgg aagaggcagc ccggcgcatg gtggaaggtt tttttgcccg
     7261 cttcaacgcg gccgtggaac ccggcgcgcc cggcatcgca gccggcgagg cgcgcgtcac
     7321 cgaaacaccg ggcgggctgt ggctgacgcc gctgtgggct tttctcggcg gtttcggcgc
     7381 cggcctggtg gcgatcatcg ccgccaccat tctccagtga ggcgattact ccgccgaggc
     7441 gtgctgcacg taggtgccgc gcggttgggc gggggcaatt tcgatatcgg tcacgtagac
     7501 gatttcacag gcgccgtcac cggtgtcgtc gcgcaccagc gaggtcgtcc agcgatagcc
     7561 gtcggtaggg cgataggcgt tgaccagtac gcccgccaga tgcaccacgt catcgggttg
     7621 caccatacgc agcttgcgct cgatttccgg cgtggcggga atcgtatgga cgttggtgga
     7681 ggattgttcg atggccgtgc gcggcagcgg cgcgcgcgcg gtttgccagt agaagaagcg
     7741 ctcgccctgg ctcatctcga tctgcgccag cacgcggcgg tcggacatgg ggccccagcc
     7801 gatcgcgata tccaccggcg acaactcggc gacggtatcc gactcataga cgagtcgacc
     7861 cagtacgcgg cccgtgactt cgaagcgcgc caccggcgtc agttcaaagc ccgcacgtgt
     7921 cagggtgggc tgctgtccga ccagcatctc ctggtagggc agttcctgaa tgatcgaagc
     7981 tgcgtgcgcc gtcagcgtga cattccgtgc cgcgatcgca cccgtgccca ccaggagcag
     8041 acacaaagtc agaacccgaa cgaacatttc cctccaccta atccataaaa ctcaacgatc
     8101 taggtgaatg tgcttgagta acgtcgtaat gtcgacaggc gggttagcgc agatgtgcac
     8161 taattcccct cgcgatcgga ttccgccttc cagccagctt gactcgcgcg agatgtccat
     8221 gacggaaacg ggatgcaatg cgttccgcat ccggattcgc ctgacccgac gactgcaatt
     8281 ccccgatgaa aaccgcgctt gcgcgcctgc tctgacggcc ttgcgcgtgg agcggcggtt
     8341 ttcctcaccc gcttgaactt taccgcaata aacgccgcgc aacaggcccg cggcgctcag
     8401 acgcgtttgc gcgcctgcgc gtagacgaag ctgtcgaagg tctgctcggc gacgcggaac
     8461 cacaaatggc tttcgtcgcg atagccgcgc cacgaccgat agattttgtc gaacagcggg
     8521 tcctgccgcg ccagttcgtc ataaagatca aaggcggcgc gataggccgc ctcgagcacc
     8581 ggcttgggaa aggcgcgcat gcgtgcgccg ctgcgcacca gttcacgcag cgcaacagga
     8641 ttgcgcgtgt cgtattgtgc ggtcatgacg gtgttcgttt cgttcgccgc gacttccacg
     8701 gcggcctgat acgccggcgg cagcccctgc caatgcgccg cattgacgag caccgacaat
     8761 tgcgccgagc cttcccagaa gccgggatag tagtagtacg gcgcgacctt gacgaagccg
     8821 agcttggcat cgtcgtacgg cccgacccac tcggccgcgt cgatggcgcc gcgctcgagc
     8881 gcctgataga tttcgctgcc ggcgatctgc tgcggcacca cgccgagccg cgccatcacc
     8941 tgcccggcga tgcccgcgat gcggaacttg agcccctgca gatcggcgag cgtgttcacc
     9001 tccttgcgga accagccgcc catctgcgcg ccggtattgc cggcgggaaa ctgcacacag
     9061 ccgtagcggc cgaacaactc gcgcaacaag gcgatgccgc cgccttcgta catccacgcg
     9121 ttctgctggc gggcgttgag tccgaagggc agcgtggtgt cgaaagcgaa gcccggatgc
     9181 ttgccgacgt agtagtagct cgcggtatga ccgcattcga tggtgccgct ttgtaccgcg
     9241 tccagcacct gcagcggcgg cacgatttcg ccggctgcga acacccggat ctggaaacgc
     9301 ccgtcggtga gttgcgcgac acgccgggcg aaatgctcgc ccgcgccgta cagtgcatcc
     9361 aggcttttgg gaaagctcga cgccagccgc caatgcagtt cgggttgcgc ctgcgcgtaa
     9421 gccgcggccg gcgcggccag cgcaagaccc gcgcccgcgc gcttgagaaa cgaacgtcgc
     9481 tgcaccgccc tcccctttgt tgtggttctg ctcaggcgcc ggcgatggtc atgccgtcga
     9541 tgagtatcga cccgcagcgg cgcgaaccgc gcaccagcgt gtcgccgccc accgcgacga
     9601 tgttccggta catgtcacgc agattgccgg cgatggtgat ttcctcgacc ggatactgga
     9661 tctcgccgtt ctccacccag tagcccgctg cgccgcgcga ataatcgccg gtcacgagat
     9721 tgatgccctg gcccatcagc tcggtgacga gcaggcccgt gccgagttcc ttcagcatgc
     9781 cctcgaaatc gagcgttccg ggcgcgagga tgaggttgtg gttgccgccg gcgttgccgg
     9841 tcgtctgcat gcccagcttg cgcgccgaat agacgctcag gaagtagcct tgcagcacac
     9901 cgtccttcac cacgtcgcgc gggtgggtgg caacgccgtc gtcgtcgaaa taggaactcg
     9961 catgcgcgcg aggcagatgc ggctcttcgc gcaactgcac cagcggcgaa aacaccggct
    10021 tgcccaggct gtcgagcagg aacgaggact tgcgatagag gctgccgccg ctggccgcgc
    10081 cgacgaaatg cgcgagcagg ccgttggcca ccggcgcctc gtacagcaca cgcgcctcac
    10141 gcgtgctgat cttgcggctg cgcaggcgcg ccagcgcgcg acggccggca tagtcgccga
    10201 tcgcttcggg cgaagcgaga tcggccggcc ggcgctcgga ggaataccag tcgtcgcgct
    10261 gcatctgctc accttcggcg gcgatgacgg cgcaggcgat gtaatgacgc gaactgcgaa
    10321 agccgtccag aaagcccgca ctgttgcccg agatgaattg cgactgctgc gcgctgaggg
    10381 tcgcgccttc ggaattgacg acgcgcggat cgagcctgaa ggccgccgcc tcgcaggcac
    10441 gcgccgtttc gatggcctgc tccacggaaa tgtcccaggg atggaacaga tcgagatcgc
    10501 gcgattcgcg cgcaatgagt tcgggatcgg ccgggccggc cgcttcgtcg gcggcggtga
    10561 agcgcgcgat cgacaatgct gcgtcgatgg tgtcctcgat cgccttgggc gacagatcgg
    10621 tggtgctggc gtagcccttc tgctgtccga tatagacggt gacgcccaat cccttgtcgt
    10681 ggttgtattc gatggtttcg acttcgccgc agcgcaccgt cacgctctgg ccgtagcctt
    10741 ccgacacgtc cgcttcgcag gcgctcgcgc ccctctcctt cgcgcgccgc agcacgtcgt
    10801 cgatgatgtt gcggaaggcg tcgagcgtat aggcaaagcg ttcgttgggc atgcgggatg
    10861 gggaaagtcg aatgtggcgg gattggccgc tattatacgg actccccttt ccccgaccga
    10921 tcatgttcga cgacgaaaaa ccgagtaaaa cgcggcgcaa gaaagacatg catgccctgc
    10981 aggccctggg ggaggcgctg gttgaactgc gtcccgaaca gttggaaggg gtggacatgc
    11041 ccgaagcgct gcgcgacgcg gtgctggagg ccaaacgcct cacccgacac gaggcgcggc
    11101 gccggcagat gcagtacatc ggcaagctga tgcgcgaagt ggacgccgcc ccgattcgcg
    11161 ccaagctgga cgaactggcg ggccgctccg caaccgcgac cgccgcactg catcgggtgg
    11221 agcgctggcg cgatcgcctg atcgaggacg agcaggcgtt caacgaattc gcccagacct
    11281 acccgcatgc ggatttgcag cccgtgcgca ccgcggtgcg cgaagtgcgc aaggagcgcg
    11341 ccgcgtcgcg tccgccgcgc cactaccgcg agttgttccg gctgctcaag gcgatcgacg
    11401 aatcgcgcgg ccatgcggat gcggattgaa ggctcccgga cggcgttggc attgatgttt
    11461 gccgcgggca cggcggtcgc gcaaaccgac gctctgcagc cggggcttta tgaagtcgaa
    11521 ctgcgcacgc agttcagcga cgcacaaggc gaatcggtcg tgccggtcag cgtcgtgcgc
    11581 cgctgcgtga gcgccacgga aatccaatcg cccgagcgcc tcgcgccgaa cttctccgcc
    11641 gcggcggcgt gcgtctggtc ggcgcatcga ccggagccgg gcggcgcatc ctggacagtc
    11701 gactgccagg gcgacccggt catgcgcggc gaggcgcacg tgcgctggac caaggaccgc
    11761 tacgccggcg aaacgcgcat ggtgctgcgg cgcggcgccg aacggatgaa catgacgcag
    11821 caatacaccg cccaccggct gggcgggtgc tgaaaggagt acaggcatga acatgctcga
    11881 ccggccgctg aaaatcggcc tcgtttcgat cagcgatcgc gccgccaccg gcgtctatca
    11941 ggacgagggg ctgcccgcgt tgaaggaatg gttcaagacc gcgctcgcca cgccttggga
    12001 gacggtagag cgactcattc cggatgagca aagcgaaatc gaacgcacgc tgatcgacct
    12061 tgtcgataac gaagcctgcg atctggtact gaccaccggc ggcaccggcc ctgcgccgcg
    12121 tgacgtgacg cccgaggcaa cgctcgccgt ggccgacaag gtgatgcccg gcttcggcga
    12181 acaaatgcgt caggtgagtc tgaaattcgt gcccaccgcc atcctgtcgc gacaggtcgg
    12241 cgtggtgcgc aagaaggcgc tgatcctcaa tctgccgggc cagcccaaag ccatccgcga
    12301 aacgctcgaa ggcgcacgcg acgccgacgg caatctgctg gtcaacggca ttttcgcggc
    12361 ggtgccctac tgcatcgatt tgatcggcgg cccctatgtc gaaaccaacg agaacgtcgt
    12421 caaggcgttc cgccccaaat cagcgcagcg tgcgcgtcaa ccctagaacg agaacacgat
    12481 gtccgagctc cttcccgcag tcgaaatcga aaccgcgccg aatccgcgca ccagcatcat
    12541 ctggatgcac ggcctgggcg ccgacggcaa cgatttcgtg cccatcgtca acgaactgga
    12601 tctgcccgat acgccgatcc gcttcgtctt tccgcatgcg ccggtgatgc cggtgaccat
    12661 caactacggc catcgcatgc gcgcctggta tgacgtgctg agcagtgact tcgccgagcg
    12721 caccgacaac gaaggcgtgc gcgcctcgca gaagctcgtc gaagcgctca tcgcgcgcga
    12781 gaaggagcgc ggcagtgcgg cggaccgcat cgtgatcgcc ggtttttcgc agggcggggc
    12841 aatggcgctg cacaccggcc tgcgccatgc cgagcgtctg gccggcatca tggcgctgtc
    12901 cacctatctg ccgattcccg atgcgctaac gcaggaaggc agcgcagcaa atcgcgatgt
    12961 gccgatcttc atggcgcacg gcgatcagga ccaggtcatt ccgatcgagt atgcggcgca
    13021 gtcgcgcgat ctgttgacca cgcagggcta tcaggtcgaa tggcatgcgt accacatgcc
    13081 gcattcggtc tgcatggaag agatcgccca tatcggcgcc tggctgcaac gcgtactggg
    13141 ctgatgcgca aggtcacgct gaccgcgctg gcgggggcgc tggtgctggc cgccatcgcc
    13201 ggctatttgt acatgcagcg cgaacaattg ctgcaacggg cgatcgagca ttacggctcg
    13261 gccatactcg gcgcttcagt cagcgtgtcg agcgtgcatt tatcgccggc cgatggcgac
    13321 ggtgcgatgc gcgggctgcg aatcggcgcg ccgctgggtt tcgcgcgcga cgtggcaagg
    13381 gcagaggcga ttgaattgtc cgtggacccg gcgacgctcg ccgatccggt ggtgcatatc
    13441 cgccgcatcg tcctgagcgg cccggatatc gcatacgaac agggccgctc ggggaccaat
    13501 ctggaggcgc tgcagcggaa tgtgcggcgc tacatcggtg aggcgtcggc ggagagccgg
    13561 tcggcaaagc ggttcatcgt cgatcgactg gtcattcgcg acgcgcaact cacttacatc
    13621 cccctcagcg gcgtaagcag cgccgccgcg accatgcggc tgccggacat tacgctacgg
    13681 gacatcggca aacggcgcgg tggcgtaacg gccggcgaat tgtccgggat catcgtggac
    13741 gccttgatcg cccgcaccgc cggtgcagtc ggtcaaagcg tgctggatcg aacggtcaac
    13801 cgtttattga ggcggtgaaa tcgatcacct gcgtttcgcg acaggcgatc aattcgtaat
    13861 cggagccgcg ctccatcagg cgcgtcggcg cgaacagccg ttcgccggca gcggtttcga
    13921 tgcgatataa ctggcccggc gcatagacgc cgggccgcag cagcaggcgc gtctcctctt
    13981 cgcggcgcgg tgcgggcagc aggatgccta cctcgctgtc caggctgagg ccgtactcgg
    14041 tcttgatccg cagttcgacc ggcatggcca cgcgggacag cagttgaata ccgacgttgg
    14101 tctgctgcgc cgtggagcgc gccaagcgac gcacgatacc ggccgaccag tgtgcgccgc
    14161 cctcgtaatg cagccccagc aggcaaccga tgtgcagcca ctcgtccttc atggccggta
    14221 cccgggcgcc gaaaccgccg gcactcatgt cttccatgat ccagctttcg atctcctcgc
    14281 cggccggcgc ggcgcccggc ggcagctgca gcacgtccag cacgccgtcg aagccgcgcg
    14341 caaccgtcag ccgcagtttc gccgcctgac ggatggactt gcgctcgggc agcttgggcg
    14401 accagtgctg ctcgagatgg tcgatgacgg caaggatgcg ctcagtttcg taagtgccgc
    14461 ccaggttcac ggcgctcggc acggcgccgg aagcgcggat ttccgcggcc agggcgagca
    14521 gatcctcgtg agcggcgccg gcgccgaagt agcccaccgg ccccttggca ccgcccgaca
    14581 ccggtccacg gcgaggcgct gccgcagcgc ccagatccac ccagtaaggc gtttcgggac
    14641 cggcctcggc cgccgccgcg aacttgggcc cgaactgcgc aatcaggcga tctgccaact
    14701 ccatgtccac cggcgccagc gcccccggcg agcaggccgc gaacatgaac agccgcaaca
    14761 actcctgctg catggaggac tcgcccggca cattcggata taccaccacc gacatctgcg
    14821 agacgcgcgc ctgttcggac gccgcgtaaa cccgcgacac cgtcgaccac agcccgtcgc
    14881 tcaccgggcc atagcgcaaa tgcatccact tcagttggcc ggtaaggctg cgcagtgccc
    14941 gaaccgcgac cagggcagtg gttgcacgag ccgcatcgcc cggtcgcagg gcaaccatct
    15001 gctccaagac cgcggcgaac gcgcgcgccg cctcctgcaa gtacgcgtcg acgtgattcc
    15061 agatgcgcat ttcctgctgc ttcagcagcc gcgtcgaccc caggtagtcg cgtgaaagct
    15121 tgcgcaaatg cggctgcgcc gcctcgtcga tcaacaacag cgcctgcgaa cggtggtccg
    15181 gccgaaaatc ggcctcggca gctaccgact gcagccaatg cgtgaggtcg tctatcgcgg
    15241 cttgcgcttc gccctcggcg atgccgccaa gcagttcgcg cgcgcctttg aggtccccga
    15301 aagggtgatc ggacgacttg cgattgaaga tgttcagcat gcttgctttt tatatttata
    15361 gtgcccgagc aggacgggac tttagcaaag gccaagccgc aagcctaggc gcatcgccca
    15421 acggcgccgg cgaccgcgag gccggtagtg ctgcggcaag aggaagcgcc caccccatgc
    15481 agcctgaagg ggcgccggcg acccttcggc gccgatctag cgcttggcgg cgatgcgccg
    15541 caggatcgtt tgcgcctcgt ccagcgccat cgcctcccgc cggcgctcga caagctccaa
    15601 ctcctctttg agccgcgcca cgaacttctc ggccagggcg tgggactcgt gtggattggg
    15661 agcaccgaaa tcctgctcca tgcgagcctc ttcggcttgc agacgctgct cggtggaact
    15721 caagtgttta cgatgagcac gcatgtccct tcctctcccg tgtagccctg atgcggtggg
    15781 cccacaccga aacattactc tatttccgtg cggttcgcat acggctcggc gcaggtcgcc
    15841 gacaacatat gtggcacgca cttttccgga cgccggaacg gagcggagaa ggacagaaaa
    15901 cggaaccgca aggactgcgg agactggggg gtggtgctgg tgagaggagt cgaacctccg
    15961 acctactgat tacgaatcag ttgctctacc aactgagcta caccagcaca tcgacccggc
    16021 ccgaacgggc gcgagatgcg gattatagca gcgccttgac gaattcccag cgcttccgaa
    16081 gccgctctgg ccctccaact caagcgccca tcccgggccg gccccgtcaa gaatttcgcc
    16141 agcccgattg cgcccattgg cgtgactttt gaccggtcgt cgcatggaca tctcaccact
    16201 cgttcggcgt ttcgcgccac taatcggttc cttgcggtcg aggccaggac ggctcactac
    16261 atttacgcca agcgaaggag tcagaaatgc gtccacaacg cggattcacc cttatcgaag
    16321 cgatgatcac ggtcgcggtc attgccattc tttccatgat cgccatgccg gcttacaccg
    16381 actacgtcct gcgcggaaag atttccgaag cggttcaggg actagccgat gcgcgcgtca
    16441 aacaagagca gttcttcctg gataaccgga catacgggcc ggcggcaaca acgtgcggtg
    16501 ctccgttacc tcaaaacact aaacactggg acttcagttg ttccaatgtc tcgaccacta
    16561 cgtacacggt gacggcgacc ggcaaagcat ccggcggaac cgccggcatg agctacagca
    16621 tcgatgaaga aaacgtccgt agctcggtca tcaatgcatc cgtaggcccc aagggttgga
    16681 ccggtcaagc aaactgctgg gtcgtgcgga agggcggtgt atgttgatgc acgggcgcgg
    16741 tttcacgctg atcgaactga tgatcggcat cgcgctcatg gcgctgttgc tgacgttcgg
    16801 gatgccgagt ttctcgacca tgatgcacaa catgaaggtc agaagcatgg ccgaatcgct
    16861 cgttcatggc ctgcagaacg ccaaggccga ggcgattaag cgcaatcaac ccgtcgagtt
    16921 cattctgacg acggacgaaa ccgaccccga cgacgtcgcc tcgtatacca aatcaacgaa
    16981 cggtagaaac tgggctatac gcatatcgga tggcggaggc tacaccttcg ttgaagccga
    17041 agcgggtaac gaagccgcca ataacccggt ccaggtggcc gccaccggag gcgggttcga
    17101 tggaatcgtc acattttcgg gcttggggac cacgaccctg gcaagcgatg tggcgctcga
    17161 catcacgaac cccatcgccg gcgcctgtaa gacggccgac ggcgatgaac cggtgcgctg
    17221 tctgcgcgtt gttgtcacca cctcgggacg cgtgcggatg tgcgatccct cggtcaacgc
    17281 agcgggtggc gacacgcgtg cgtgctaagg gctcactgat atgacgcgaa aatttcaatc
    17341 gggtgtcatg ctgatcgaag cgctgatcgg catcctcata ttctccatcg gcattctggc
    17401 gatgatcgcg ctacaggcag catccatcag cgccactgca gacgcgcaat accgggttga
    17461 agcagtcaat ctcgcaaacc agatgatggg gcaaatctgg gcaggtgtgg accgcagcac
    17521 gagtgcggcg ctggagaact cgctgctcac tttcgaacac ctagccgacg aaggcgacga
    17581 taactgcaat ttcgagggaa ccgagtccac caacaccatg gtgacgcggt ggcgcaatct
    17641 gatcgtcacc ggagcggcgg acggcaccgg cactcgcccg ctactccccg gggctagtaa
    17701 taccgatcaa cagatcgacg tcaatatcgg cgcaaacaac caggtgaaga tcaccgtctg
    17761 ctggcgcacg ccaccggaca atgtcaccca tcgtcacgtg ctcctcgcga acgttaactg
    17821 agtcatcgac atgagcactt tgctatcgaa aaccggccga cacggcacgc cgaacgagcg
    17881 aggcatgagc cttgttgaaa tacttgtcgg catcctgatc ggaatgatcg cgatcatcgt
    17941 tatcttccag gttctgtccg tgtacgaaga ccgcaaacga accgcagtcg gcgctggaga
    18001 cgcgcagacg gcaggatcca tcgcactcta catgttggat cgcgatctgc gcttggccgg
    18061 atacgggtac agtgatgcgg aaaccatggg atgtaacgtt gttggccacg atgcaaccac
    18121 cggtgccgca ctcaatttca cgcttgcccc cgcgattatt tcgaaaccca atggcgctgc
    18181 agggccggat gccatcatct ccctcagggg cagctctcct ttgtttgtcg cacgcaaagc
    18241 gttcacgagt tccgcagaca ccgaaaagat catgcaggcg ggccatcgac cgggaatact
    18301 tcgcggcgac attctcgtcg tttccccacc gacccctggg ccatgcgcgc ttgtcgaagt
    18361 gacggataac accgccggag acaaggtaac catcctgcat gaaaccggca gctatacctc
    18421 cgactactcg gcaggcgcgg tggcggcacg atacaacaag gacggcggcc tcggcgtcgc
    18481 gtttactcaa ggcttcctat acagccttgg tcccagccct acacgtaccg tatggtctgt
    18541 aaccggcaac cggctgtcgt acaccgacac cctgcatgcc gatcaaggcg ccatcgatgt
    18601 cgcagacggt gtagtgaatc tgaaggccga gtacggcatc gacgcgaatg acaacaacga
    18661 actggagcag gacgagtgga ccacaaccga cccgactacg gctgccggtt ggagagcgat
    18721 tcgcgccata cgcgttgcaa tgctgatgcg tagcagccaa tgggatccga atgtctgcag
    18781 taccaatcct caatggacga gcggcgccag cggcacgcca accttgacga acttcgtcat
    18841 gcgcaatgta gacggcacta acgattccta cagcgattgc aatggcacta atgcggaccc
    18901 gaacaactgg cggacctatc gctaccgcgt gtacgaaacc gttattccac tacgcaacat
    18961 gatttgggga actgcgccgt gatcaagcaa ggacaccaac cgcatagttt gaagcgcagg
    19021 caacgtggcg tcgtcctttt catagcgctc atcgtcctgg tcgcactaac gcttgccggg
    19081 ctcgctatgg tccgatcctc cacgagcggc atcctcgtcg ccggaaacct ggccttcaag
    19141 cagaccgcga cagcctcgag cgacgccggc gtcgaagctg cgcgcgcctg gctgcgcacc
    19201 aagggccctg ccgaactcga agcctctgac gcaacgcaag gctactacgc cacttgggag
    19261 ccgacgttcg atcccgcaac gtcggccatt tgggagccca acacctcgcg ctcggtgggc
    19321 gctgccgatg acgccggcaa cactgtgcgc tacgtcattc acaggatgtg tgatgccgcc
    19381 ggagctctga aagacgtgga ttgcgtcacc gtcgcaagcg gcggactggg cggatccaaa
    19441 ggaggtgggt gttatggctg taaggctttc acgggatcgt ctcagcctta ctttcgcatc
    19501 actgtgcgta ccacggggcc caagaacacc gtgagctacg tgcaagcgat gatgtattaa
    19561 gactattcga ggcagaaaat ggccagaaaa aacctcaaca gccacgcgcg caaggatcgg
    19621 cttgcgcagc tctctttagc catcgcacta tgtttttgtc tgcccgccgg cggcgccgag
    19681 cttacccagc tggcagatgc gccattgtcg agttccgcgg cggagcaggt gccgccgaac
    19741 atcatgttca tcctcgacga ctcaggctcc atggcgtgga cgcacctgcc tgacatctcg
    19801 aacagcagca cgatcgaaaa caatacggtg ggataccgga accatctgtg caatccgatg
    19861 tatttcaatc ccgatgaagt ttataagccg ccggtcgata aagatggtaa agcctatgac
    19921 gatatggact tcgacaaagc tctgtacaac ggctttattc cgaccgacaa aagccccgac
    19981 accggcacat ccaaaatcaa tctcggctcg cagtttcgtg catacgatac aaagaccact
    20041 gccaacatgt cgggcggtag cgtcagggcc agtgccgaag ccgcacacta ctacaagtgg
    20101 aaaggcgcag gcacgccgag tccgaccgag tgcgacaagc ggatcagcaa cccgggaagt
    20161 aattgggaaa aagtaacagc cgctgatttc actgatgacg acaagacgaa cttcgccaac
    20221 tggtacagct attaccgtac gcgcatgttg ctgatgaaga ccgcggccgg gcgcgccttc
    20281 agcattctcg acaagcacta ccgcgtcggc ttcctcacga tcaacccgat gaagagttcg
    20341 ggcaggtccg ggcagaccat tgatgaaaag aagtacttga aaatctcgga gttcgagtcg
    20401 gaccataagg aagactggta cgacattttc tatagccaga ttgccagcgg cggcacgccg
    20461 ctgcgcgaag cgttgtcccg cgcgggccga cacttcgcag gcaaatcaga cggcatcaac
    20521 aacgggatga ccgatgaccc gctgctctat tcctgccagc ccaacttcgc cattctgacc
    20581 acggacggca cctggaacgg caaccccgga caaaccataa atggcggctc catcggcaat
    20641 caggacggcg acctcgcttt cacgccacgg ccgatgtacg acggcggaga agtcgagaag
    20701 aaagacatca ccacctacac ggaatggcgc taccggagcc gtaactccgg gaaacctaag
    20761 tgttcagaag atgagcagag cgcgacgaag gaaacccgca ccataacggt cacgactacc
    20821 actacgcagt acggtacttc gagcacaaag ccgaccacag gatccacgaa gtcgcagaac
    20881 atctgcttac cgaacggcgt ggatcgaacc accgattggg ctatcgaaag cggctccgaa
    20941 acgaatgaga cgataaccgc cggcaaaggc agcgcctcca acacgttggc cgacgtcgcg
    21001 gagtactact accgcacgga cctgcgttcc gacacggcaa cgcgcaccgg gcccaacggt
    21061 gagcagatca acatcggcgc cgataacgtt ccggtggccg gcaccggccc cgaagacgac
    21121 aaggctacgc accagcacat gacgacattt acggtcggct tgggcgtatc cggacgattg
    21181 aacttcgatc cgaactacaa gtcggcgaaa accggcgatt tccaggatat acgcgacggc
    21241 aagaagggct ggcccgatcc gagtagcagc gacctggcca agaccgacga cctctggcat
    21301 gcagcggtga acgggcgtgg gcaggccttc agtgcgcagg atccctatac cctcgcgaca
    21361 tcgctgcaag gcgccttgtc aaacattcag gcgcggatcg cttcctcggc cgcagcagcc
    21421 acctcgaact tggagccgac tgaaaccgac cgcctggtat tcctgcccaa atacgtttcg
    21481 gcaaagtgga cgggtgagat cgaagcgcac ttgcttaatc tgacgacagg tgaggtcgaa
    21541 gacgacctca cgtggtctgc acaagcggga ctcgacggtc agactcaagc ggattgcgac
    21601 gatcggaata tctatctgta ccgcaaaggc gcgacaaaca atctggtgga tttcgcctgg
    21661 gataccaagg cttgcgaaga caactccatc accacagggc tggacccgga cgagaaagct
    21721 ttcttctcga gcaaccatat caacgggctg tcgcagtggc tcactatgag cgacggaacg
    21781 gacggaacag caaaccagaa ggcggctgct gcggatgcga acctggttaa ttttgtgcgg
    21841 gggcagcgcg gcaaggaagg atatatgccg aacgacgccg acaagctgta ccgagagcga
    21901 gagcatgtcc tcggcgatat tgttggcgct cagccggtat atgtcgcggc cccggcattt
    21961 gcgtacacgg agaacggcta ccccaccttc aagaccaaca acgcttctcg cgcgggcatg
    22021 gtgtatgcgg cgagcaatgg cggcatgctg cacgcgttca aagttgggac attggccaac
    22081 aactttgccg atggcggcaa agaggcgtgg gcgttcatcc cgcgcacggt actacctaac
    22141 ttgtacaagc tggcagatac caattgggca agcaaacacg agtttctggt cgatggcagc
    22201 ccggttgtcg gcgacattta cgacacgtct gccagtgcgt ggaaaaccat actcgtcggc
    22261 gggctgaaca agggtggcaa ggcgtattac gcgcttgata tcaccaaccc caacagtccg
    22321 aaagcattgt gggaattcgc gcacagcgat acctgctatg acaaggatgc tccggctacc
    22381 cactacgccg attgccacat cggttatacg tttggcaatc cgatcattac gaagatcgcc
    22441 ggcaaagacg cgagcaataa cgaatggcaa aagtgggtcg tactggttac ctcggggtat
    22501 aacaacgtca acgaacccgc taaagcggga gatggggtgg gctaccttta tgtgctcgat
    22561 gcggtaagcg gcaggatctt gcacaaggtc agcaccgggt acggcgatag tggcgccggc
    22621 gacaactccg tcggcccgag cgggttggca aagatcaaca actgggtcgt tggcgatgcc
    22681 tattttgacg ccaaatcatc ggcagtatac ggcggtgact tgttggggaa catctaccgg
    22741 ttcaacgtcg atgtcgagcc gccgaccgtg aagctgatcg gaacagccaa agacgactcg
    22801 ggcgccggac aaccgatcac taccaagccg gaattggccg aagtcgggac tccaccgtcg
    22861 ccgtacgtgt atgtcgccac gggccgttat ctcgcgagcc ccgacgtctc gaatacacag
    22921 actcaatcgc tctatgcgat caaagatcct ggcgcagccg cggggtacac gaatctgcgc
    22981 gactctctgc tgcggctgga gatgaccaac gtcaacgcgg ataatgagcg attcgtaaat
    23041 tgctttgaga actgcagttc cacaaacggc tggacggtgg atttccctga ctccggtgag
    23101 cgggttaacg tggacatgaa gctgcaactc ggaacgctga tggtgttgag caacgtgccg
    23161 aagaacaaca cttgcgagcc cggcggctac agttacgcga actacttcca ctatgcgagc
    23221 ggacagtctc cgttgggcat cggccagcct ataggcggaa aactgtcggg ttcgctggcc
    23281 gttggattca atgtcatccg cctgccgagt ggaaaggtca tcgtcatctc gacggatgct
    23341 gcgggcaaca cgtcggccag cgaagccccc atcggttcag gtgcaccgac cggaaagcgc
    23401 gtttcctggc gtgagcttct gaactgagcg ttgtttccct caaaggcggc cgaatggccg
    23461 cctttttttt tacgaccggc gggcacaaaa cagacgctgc tgcggatgcg tcaaccgagg
    23521 gacagtccct tcggcagcgg gaacgacaca ttctccgccg ccccgtccag ttgctggacg
    23581 cgctgtgcgc ccagttgctt caggcgctcg agcacgcgat tgacgagcac ttccggtgca
    23641 gaggcgccgg cggtcacgcc gacccgcgtc ttaccggaca cccattgcgg atcgatctgg
    23701 tcttcgtgat ccaccatgta cgcgggcacg cccatgttct gcgccacctc acgcaaacga
    23761 ttggagttcg agctattggt actgc
//
LOCUS       DATGOU010000080        15617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_5095751, whole genome shotgun sequence.
ACCESSION   DATGOU010000080 DATGOU010000000
VERSION     DATGOU010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15617
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5095751"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /gene="recD"
                     /locus_tag="VKZ48_04945"
     CDS             <1..1331
                     /gene="recD"
                     /locus_tag="VKZ48_04945"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HLU77233.1"
                     /translation="PGTGKTTTVMRALAVLAETSAKPLRIALTAPTGKAAARLQEALR
                     HARAALALPAQIDTQLPQEASTLHRLLGTVSGSTRFRHDRTRPLPLDVLVVDEASMID
                     LALMAKLLDALPPHARLIVLGDKDQLASVEAGAVMHSMCAGPDAYTSETAALLRKLTG
                     YEVPAAEVISPLADAVAMLRQRYRFASGSAIAALADAAREGDVATALRVLGESDGTEV
                     AWHAASEYAAVVAALTDRYEPLVRAAHRADATPEALYALLREHGVLCAHRAGPYGAET
                     LNRAVELELRRRGLIDEPGTWYRGRPVMIRRNDYAARLYNGDVGVVCADADGRLGVYF
                     PDAAGRLRRYATARISASCESAFAVTVHKAQGSEYGAVDFVLPAQSSKILTRELFYTA
                     VTRASARVAVWGSADIVRASLTGSVTRDSALANRLWFKPEANTPVQGTLF"
     gene            1459..2355
                     /locus_tag="VKZ48_04950"
     CDS             1459..2355
                     /locus_tag="VKZ48_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLU77234.1"
                     /translation="MSAGIDIPQLDPVAPAVPPLRHVKLDRLVNMAVERTREGMRARH
                     QQLHVEAMAAPIVVCGDRDTLAEALARLLDTASNAAGEGGVVTLSVSQIGLNATLKVG
                     VCRQMAGASDSDTDCDWTAVERLIEAHGGTVSARTHAEGGAEYALLLPVLQQDEAPVT
                     RRASLIAGPEAPVCRVLVVDDSADAADSIACLLSIRGHEVRVAYDGPSAYAKLLEWEP
                     DVVLLDVQLPGMDGHEIVDAVRARMHEGRTPPQFIALSGYGPCARGKASFDLYLTKPV
                     DPSILMKAMERCARKSAAQVRA"
     gene            2496..2765
                     /locus_tag="VKZ48_04955"
     CDS             2496..2765
                     /locus_tag="VKZ48_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77235.1"
                     /translation="MEPQRPPQIPSRIIERPDGFYWRDDSDLEYGPFATLSEALHDME
                     LSGDVEAETPDFESLEEVEAELGVGWIDPSTGEPGGEGAPRLEDH"
     gene            complement(3028..3423)
                     /locus_tag="VKZ48_04960"
     CDS             complement(3028..3423)
                     /locus_tag="VKZ48_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HLU77236.1"
                     /translation="MLMLDDTEIREIEDGLQKRRSQLIEEISEKLRAARDPTSSEQAD
                     ELIEVGDAAIADLLTHTSLAESQRDVQELQEIEAALARITDGSFGVCAQCGKEIEAAR
                     LQAHPTALRCIHCQEEYERTHAGPATPTL"
     gene            3529..4383
                     /locus_tag="VKZ48_04965"
     CDS             3529..4383
                     /locus_tag="VKZ48_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLU77237.1"
                     /translation="MTEHSFESGTQTFVTDVLEASRRVPVVVDFWAPWCGPCRALTPV
                     LEKLAQEYGGQFLLAKINTDEHPEIAAEYGVRGIPNVKAFVDGGMVSEFTGALPEAGV
                     RQFLERILPGPSERARRAAAASLGEGDFDAAEQQLREALRLDERNDAARIDLAELLVA
                     RQAFEEAEGLLGELLPIHRDARVEQIEARIGVWRKSQSLPDATALQAELDAHPENLEA
                     RLAYAERLVVEGRYAQALDAFLQVVQGGRGELRERARQGMLQVFNLDVGDSALISDYR
                     RRLASALH"
     gene            4627..5238
                     /locus_tag="VKZ48_04970"
     CDS             4627..5238
                     /locus_tag="VKZ48_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HLU77238.1"
                     /translation="MRGDVPLILTLDNHGVPHRWVTWQQACFYYAKNLVAWTLGDVAF
                     TAHGGISRLTGERSRITGHSIIAIKGKAMAIKGFQQVPPLNNRELFRRDRHLCAYCGG
                     EFSFVRLTRDHIRPVSRGGQDTWMNVVTACRHCNGLKRNRTPEEAGIQLRYAPYVPNK
                     AEYLILTNRKILADQMEFLVQHVAANSRVFDLATRRSEDPLAA"
     gene            5252..5899
                     /locus_tag="VKZ48_04975"
     CDS             5252..5899
                     /locus_tag="VKZ48_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HLU77239.1"
                     /translation="MTAVPAVPPVALTPAYWARAKRELARNDPLLDAVLRAHPGAVLR
                     SRGDAFQTLMRSIVGQQISLKAADSVWARLCLAVAPRPADVIALGHERLAACGLSQRK
                     AEYILDLARHFAEGRLHIHRWPEMDDEAVIQELIAVRGIGRWTAEMFLMFNLLRPDVL
                     PVDDLGLRRAVSELYFGGEPIGADAVRHVARDWAPWRSVATWYLWRSLEPVPVEY"
     gene            6023..6991
                     /locus_tag="VKZ48_04980"
     CDS             6023..6991
                     /locus_tag="VKZ48_04980"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HLU77240.1"
                     /translation="MKSIFLEFEQSVAELEAKIEELRFVQDDSAVDISEEIARLQKKS
                     QSLTKDIYAKLSPWQTAQVARHPQRPYTLDYVNMLFTDFEELHGDRAYADDPSIVGGL
                     ARFNGQSVMVIGHQKGRDTKEKIYRNFGMPRPEGYRKALRLMRLAEKFGLPVMTFVDT
                     PGAYPGVGAEERGQSEAIGRNLYVMAELKVPIVSTIIGEGGSGGALAISVGDVVMMLQ
                     YSTYSVISPEGCASILWKSAERAADAAETLGITADRLKSLGLIDRVIPEPLGGAHRDY
                     ATAGQNLRKALQDALRELSNHSPAELVERRFERLMAYGKYKENAGK"
     gene            6960..8309
                     /gene="tilS"
                     /locus_tag="VKZ48_04985"
     CDS             6960..8309
                     /gene="tilS"
                     /locus_tag="VKZ48_04985"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HLU77241.1"
                     /translation="MASTRKTRGSELLPVVEAALAAHVPPGARLTLGYSGGVDSAAAL
                     DALARLAAPREYTLDCLHVHHGLSPNADRWAEFAQRTAAGYGIACRVRKVNPADYPGL
                     GVEGAARAARYAAYAECDTDFIVLAHHRDDQAETLLLQLARGAGVQGLAAMPVLRPLE
                     GARSQLLRPLLDVSRAAIAAYAAGRDLEWVEDESNLDRGYARNRLRHEVLPALQAINP
                     AAVANIARAAAHLSDVAQLVDAWAGEDLMRVMREDAAVDLVALRALGEVRARYVLRAW
                     LTARGLVPPSMAELSEVLRQLEDAREDAAPRGDFGEVELRRFRDGAYLVAKRPPPPRD
                     YVAQWTGEPLWPLPELHGSLRFDVVRGAGIALRHLPSMTVRLRQGGERFRPGPGRPHR
                     RLKHLLQESAIPPWRRERLPLLYVGSRLAAVPGIGIDPEFVAAEGESGWLPRWETQH"
     gene            8502..8927
                     /gene="ndk"
                     /locus_tag="VKZ48_04990"
     CDS             8502..8927
                     /gene="ndk"
                     /locus_tag="VKZ48_04990"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HLU77242.1"
                     /translation="MAVERTLSIVKPDAVGKNVIGKIYSRFEDNGLKIVASKMKWLSR
                     QEAEGFYAVHRERPFFGDLVDFMVSGPVMIQVVEGENAIAKNRELMGATDPKKAEKGT
                     IRADFAESIDANAVHGSDSAENAAIEIAYFFPSSEIYSR"
     gene            8943..10025
                     /gene="rlmN"
                     /locus_tag="VKZ48_04995"
     CDS             8943..10025
                     /gene="rlmN"
                     /locus_tag="VKZ48_04995"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HLU77243.1"
                     /translation="MSKNLLGLDAAQLQAYFAELGEKPFRARQLMRWVHRNGEADFAL
                     MSDLAKSLRQKLNESAAVVPPRMIQGSVAADGTRKWLLDVGNNNAVETVYIPETNRGT
                     LCISSQAGCTMDCKFCSTGRQGFNRNLTSAEIIGQLWHANRVLGADADGERVISNVVM
                     MGMGEPLLNLDNVIPALNLMLDDHAYGLSRRRVTVSTSGIVPMIDRLRDECPVALAVS
                     LHAPNDELRDRIMPINRKYPLAELLAACRRYLEHAPRDFITFEYVMLDGVNDSVAHAR
                     ELIALLADVPSKVNLIPFNPFPDAGFGRSPRDVIQRFSNLLNEAGIVTTTRKTRGDDI
                     DAACGQLAGKVHDRTRRELRIARASA"
     gene            10022..10834
                     /gene="pilW"
                     /locus_tag="VKZ48_05000"
     CDS             10022..10834
                     /gene="pilW"
                     /locus_tag="VKZ48_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HLU77244.1"
                     /translation="MKRGVLVIALGALLSAGCQTVPEAQQDQIQAAREILEAGDMIVA
                     LPADAQTRAKAHTDLAAAYYELGNLAVALEETRIALRANPNYARAHNVRGLVHMDLRE
                     NAEAEQSFKRGLQLAPQDADLNHNYGWFLCQTNRADESVEWFLKAIKNPLYRTPARSY
                     AAAGHCTERSDPKEAAEFYERALRLEPNNPSVLLSFAGLQYRQGQLHEARALVSRYYK
                     LVKDPTAESLWLAHRIEHKLGDRMAANQYATELRRRYANSPQYQALQRGQYE"
     gene            10843..11784
                     /locus_tag="VKZ48_05005"
     CDS             10843..11784
                     /locus_tag="VKZ48_05005"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HLU77245.1"
                     /translation="MEEMQAEQTAAPQEEVGPGRMLAAAREARGVSVDDVAMRMKFAP
                     RQIEALEADQYDQLPGVAIVRGMVRNYARLLEIDADPLIAELEKRMRTGPITVQPADM
                     HVPIREGRKEGRMMLVLSLIALIALGAFVLDWYVRDHRIVQPAQLESDAAEEAAEQTD
                     DTVQPLDVQPASNAEPAVSTTKVAAPAAAIMPAAHVVEPDPAPAAVKEADPVDPPLAV
                     APKEPETEAVARSAPAASDKDLEMRFSEDVWVEVKDARDRLLAARLAPAGSELALDGE
                     PPFKLVLGNADAVQLTYKGEPVAIPQTRSGVARLTLE"
     gene            11787..13064
                     /gene="ispG"
                     /locus_tag="VKZ48_05010"
                     /gene_synonym="gcpE"
     CDS             11787..13064
                     /gene="ispG"
                     /locus_tag="VKZ48_05010"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006393975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HLU77246.1"
                     /translation="MSAMTRRLSRMVRIGHVAVGGTAPIMVQSMTNTDTADAAATAQQ
                     VYDLAQAGSEVVRITVNTAEAAAQVANIRERLDAMHCDVPLVGDFHFNGHKLLTQYPD
                     CAQALAKYRINPGNVGKGSKRDEQFATMIELACKYDKPVRIGVNWGSLDQDLLARLMD
                     DNARRAQPLEVDQVMREALIASALESAARAEELGLPGDRIVLSCKVSAVQDLIKVYTE
                     LAARCDYPLHLGLTEAGMGSKGIVASTAALAVLLQQGVGDTIRISLTPEPGGDRTREV
                     VVAQEILQTMGLRSFTPLVISCPGCGRTTSTYFQELAAKIQHYLRTQMPVWRADYPGV
                     EDMHVAVMGCVVNGPGESKQANIGISLPGAGETPVAPVYVDGQKTVTLKGAHIAEEFQ
                     QIVEDYVHRTYGPGGRGRTTRQNDKIIAIRAAH"
     gene            13109..14404
                     /gene="hisS"
                     /locus_tag="VKZ48_05015"
     CDS             13109..14404
                     /gene="hisS"
                     /locus_tag="VKZ48_05015"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HLU77247.1"
                     /translation="MSQTLQAVRGMNDILPDEAVLWERFEDLIRSWLASYGYRNLRTP
                     LLEHTALFRRAIGEATDIVEKEMYSFVDELNGEHLTLRPEGTAAAVRAVVQHSMLYNG
                     PQRLWYMGPMFRHERPQKGRYRQFHQVGVEALGFAGPDIDAELLLMCARLWDDLGLQH
                     IRLQLNSLGSSEERAKHREALVAYLKSRESELDEDSRRRLHTNPLRVLDSKNPAMQTL
                     IAEAPKLLDYLGAESMAHFEGVQRVLKDAGIEYEINPRLVRGLDYYNLTVFEWVTDRL
                     GAQGTVCAGGRYDGLIAEIGGKPAPAAGFAMGIERLLALLCESGENAADWLQPTPQDV
                     YIVHQGEQAGRLAFTVAEALRSLGFTVTQHCGGGSFKSQMKKADGSGAHIAVVIGDDE
                     AAAHEVSVKPLREERGQWRVSVEQLPEAITDLLYGEQGE"
     gene            14411..15049
                     /locus_tag="VKZ48_05020"
     CDS             14411..15049
                     /locus_tag="VKZ48_05020"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLU77248.1"
                     /translation="MATYDLEEQEKIEELKAWWKQYGTLIITTTALALIIIAGMRFWD
                     GYQRTQSVEANAVYNRLLQAVQADEGSKVAELSALVRDDYSRTVYASLGTFAAAKYHV
                     EKGELEAAREQLAWAAENARDAQLKAVARLRLAQVLIDLQRYDEALQQLEAEHPAAFT
                     TQYAQTRGDIYALQGKTGEARTAYQAALAATSPAERTLRELLQFKIDNLGAV"
     gene            15049..>15617
                     /locus_tag="VKZ48_05025"
     CDS             15049..>15617
                     /locus_tag="VKZ48_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HLU77249.1"
                     /translation="MRKPLRMLGRGAAGLTLVVALGGCAALNPFNWFGGSDEARKPVP
                     LTEISQSLQVRTLWQARVGSSKGFTLAPAVARGSVYAAAYDGTVVRLEEETGKEVWRI
                     DVGDGLTGGVGSDGELVVVGTREGVVIALDNDGRVLWRARVSSEVLVAPVVAGDVVVV
                     RSADSRLFALDARDGKRQWVYQRSAPALTV"
BASE COUNT         2721 a         4982 c         5257 g         2657 t
ORIGIN      
        1 gaccgggtac ggggaagacg acgacagtca tgcgcgcact ggccgtactc gcagaaactt
       61 cggctaagcc tttgcgcatc gcactcactg cgccgacggg caaggcggcg gcacgtttgc
      121 aggaggcctt gcgtcatgca cgcgccgcgc tcgcgttgcc cgcgcagatc gacacgcaat
      181 tgccgcagga ggcgtcgaca ctgcatcgtc tgctcggcac ggtgtcgggc agcacgcgct
      241 tccggcacga tcgtacgcgg ccgctgccgc tcgatgtgct cgtggtcgat gaagcgtcga
      301 tgatcgatct ggcgctgatg gcgaaactgc tcgatgcgct gccgccgcac gcgcgtctca
      361 ttgtgctcgg cgacaaggat caactcgctt ccgtcgaagc gggcgcggtg atgcatagca
      421 tgtgcgccgg cccggatgcc tatacgagcg aaaccgcggc gttgttgcgc aagctcaccg
      481 gatatgaagt gcccgcagcc gaggtgatct cgccgttggc ggatgccgtc gccatgttgc
      541 gtcagcgtta tcgcttcgcc tcgggcagcg cgatcgctgc gttggcggat gcggcgcggg
      601 agggcgatgt cgctacagcg ctacgcgtgc ttggcgaaag cgatggcacc gaggtggcct
      661 ggcatgccgc gagcgaatat gccgccgtgg tggcggcgct gaccgatcga tacgaaccgc
      721 tggtgcgcgc ggcgcaccgg gccgacgcga cacccgaagc gctctacgca ttgctgcgcg
      781 aacacggcgt gctgtgcgca catcgcgctg ggccctacgg cgccgaaacg ctcaatcgcg
      841 cggtggaact cgaattgcgt cgtcgcgggc tgatcgatga accaggcacg tggtaccgcg
      901 gacgtccggt gatgattcga cgcaacgatt acgccgcgcg gctctacaac ggcgacgtgg
      961 gcgtcgtgtg cgcggacgcg gacggacggc tgggcgttta ctttcccgac gctgcgggcc
     1021 ggctgcgacg ctacgcgacc gcccgcattt ctgcatcgtg cgaaagcgcc tttgccgtca
     1081 ccgtgcacaa ggcccagggt tcagaatacg gcgccgtcga cttcgtcttg cctgcacaat
     1141 cctcgaaaat cctgacccgc gagttgtttt ataccgccgt cacgcgcgcc agtgcccgcg
     1201 ttgcggtatg gggcagcgcg gacatcgtgc gtgcatctct taccgggtcc gtcacgcgcg
     1261 attcggcgct tgcaaaccgc ctatggttca agccggaagc gaacactccc gtccaaggca
     1321 ctcttttcta agttctttgc cctcagtcgc cggtcggcgg ctccgaattt gctgttgcgc
     1381 aggactgcga agcatgtttg catcgcggct gcagtccccc gttcgagcac gttgcaacgc
     1441 gttcaaggag acggcccaat gtccgcaggc atcgacatcc cgcaactcga tcccgtcgcg
     1501 ccggctgtgc cgccgctgcg gcacgtgaag ttggatcgtc tggtgaacat ggcggtggag
     1561 cgaacccgcg aagggatgcg tgcccgccat cagcaactgc atgtggaagc catggccgca
     1621 ccgatcgttg tttgcggcga tcgcgacacg ctggccgaag cactggcgcg gttgctcgac
     1681 accgcttcga atgcggccgg agagggcggt gtcgtgacgc tgtccgtgtc gcagatcggt
     1741 ctcaacgcca ctctcaaggt tggcgtctgc cggcagatgg ccggggcatc cgacagcgat
     1801 accgattgcg actggacggc cgtggagcgc ctgattgaag cgcacggcgg caccgtctcc
     1861 gcgcgtaccc acgccgaagg cggcgccgag tacgcgctgt tgctgcccgt gctgcagcag
     1921 gacgaggcac ccgtcactcg ccgcgcttcg ctcatcgccg ggccggaagc acccgtatgc
     1981 cgtgtactgg tggtggatga cagcgccgat gcggccgaca gcattgcctg cctgctctcc
     2041 atccgcggtc acgaagtgcg cgtcgcttat gacggcccca gcgcctatgc caagctgctc
     2101 gaatgggagc cggatgtggt gctgctcgat gtccagttgc ccggtatgga cggccacgaa
     2161 atcgtcgatg ccgtgcgtgc gcgcatgcac gaggggcgca cgccgccgca gttcattgcg
     2221 ctgagcggct atggtccctg cgcgcggggc aaagcgtcct tcgatctcta tctcaccaag
     2281 ccggtcgatc cgtcgatact gatgaaagcg atggaacgct gtgcccgtaa gtcggcggca
     2341 caggtgcgcg cgtgagcgat gaaacgctgt tgcgatatgc gggatcattc atcgccgaat
     2401 gacgttgcgt gcatagagac gtgcgctcag aatgcagtgg tcgtcaacgc gcgccgggga
     2461 atctcgccgc gatgcgtgtc aaaccggagg aagttatgga gccgcaacgt ccgccgcaaa
     2521 tcccgtcgcg catcattgag cggcccgacg gtttttactg gcgcgacgac agcgatctgg
     2581 aatacggtcc cttcgcgacg ctgagcgaag cgctgcacga tatggaattg agcggcgacg
     2641 tcgaagccga gacgccggat tttgaatccc tggaagaagt ggaagccgag ctcggcgtgg
     2701 gttggatcga cccgtcgacc ggcgaacccg gcggcgaagg agcgccgcgt ctggaggatc
     2761 actgatgtcg ggccggcgcg accctgcggg gtcccgtacg gccaagcccg aatgggcgcc
     2821 gtaaggaatc ggcggcccgc cgtttgtgcc aaattcgtcg cattctccgt gcgaccgctg
     2881 cgttggaatt cggccggtga tgagaagtgc ctgcgtcgcg ggcataggac gtgcaacact
     2941 taaagcgctt tcgccttttc ctcctccttg ggtgaaagtt tgatcggcgg cctaggccgc
     3001 cgttttcttt ggggcgtcac cgctctctta cagagtgggg gtggccggcc ccgcgtgcgt
     3061 ccgctcgtat tcttcctggc agtgtatgca gcgcagcgcg gtggggtgcg cctggagacg
     3121 ggcagcctcg atctccttgc cgcattgcgc acagacgccg aaactgccgt cggtaatgcg
     3181 tgccagggcg gcttcgatct cctgtaattc ctgcacgtcg cgctggctct ccgcgagact
     3241 cgtatgcgtc agcagatccg caatggcggc atcgccgact tcgatcagct cgtccgcctg
     3301 ttccgagctg gtcgggtcgc gcgccgctcg aagtttctcg ctgatttcct cgatcagttg
     3361 gctgcgtcgc ttttgcaggc cgtcttcgat ctccctgatt tccgtgtcgt cgagcatgag
     3421 cattgccccg ccagtgaacc gggcctcgtg cgttgcaagg ggcgcgcccg attcggaggt
     3481 tgaatcgcgc cgagcagccc ggataattcg cgtatggacg gagacaagat gaccgagcat
     3541 tcattcgaaa gcggcaccca gaccttcgtg acggacgtcc tcgaggcatc gcgcagggtg
     3601 cccgtggtgg tcgatttctg ggcgccgtgg tgcggcccct gccgggcgct tacgccggtg
     3661 ctggaaaagc ttgcgcagga atacggcggc cagtttctgc ttgccaagat caataccgac
     3721 gagcatccgg aaatcgccgc ggaatacggc gtgcgcggca tccccaatgt gaaggccttt
     3781 gtcgacggcg ggatggtgag cgaattcacc ggcgccttgc ccgaagccgg cgtgcgccag
     3841 tttctcgagc gcatcctgcc ggggccgagc gaacgcgcgc gccgcgcggc tgccgcgagc
     3901 ctgggggaag gcgacttcga tgccgccgaa caacagttgc gcgaagcgct gcgcctcgat
     3961 gaacgcaatg atgccgcgcg catcgatctg gccgaactgc tggtggcgcg gcaggctttc
     4021 gaagaggccg aggggctgct gggggaacta ctgcctattc atcgcgatgc gcgtgtggag
     4081 cagatcgagg cgcgcatcgg ggtgtggcgc aagagccagt cgctgcccga tgcaacggcg
     4141 ctgcaggcgg agttggacgc gcatccggag aatctggaag cgcggctggc ctatgcggag
     4201 cggctggtgg ttgaagggcg ctatgcccag gcgctggatg cctttctgca ggtcgtgcag
     4261 ggtggccgcg gcgaactgcg cgagcgcgct cggcagggca tgctgcaggt attcaacctc
     4321 gatgtaggcg actccgcgct catcagcgac taccgccgca ggctcgccag cgcgctgcac
     4381 tgagcgaccc gttgccgggc gccccgatcc cgtgatttct cacgccgtgg ccgactgtgg
     4441 cacaattctg gccgattgcc tgaacggaca ccgtccggcc gggattcctg caacagacaa
     4501 gggtcatggc atcgaagcgt cagcgagccc cactcgtcgt cgcatcactg cgtaggacct
     4561 gcgcctgagg acgcagcgct cctggtcgtc gcgtgtgtca cgggcgcacc tggagcggcg
     4621 actgtcatgc gcggtgatgt tccgctgata ctgacgctgg acaaccacgg tgtcccgcat
     4681 cgctgggtga cttggcagca ggcctgcttc tactacgcca agaacctggt cgcgtggaca
     4741 ctgggggacg ttgcgttcac cgcccacggc ggcatctcgc ggctcaccgg tgaacgctcc
     4801 cgcatcaccg gccactccat catcgccatc aaaggcaagg cgatggcgat caagggcttc
     4861 cagcaggtgc cgccgctcaa caaccgtgaa ctgttccgcc gcgaccggca tctgtgcgcc
     4921 tactgcggcg gcgagttcag tttcgtgcgc ctgacgcgcg atcacatccg gcccgtgtcg
     4981 cgcggcggcc aggacacgtg gatgaacgtg gtcaccgcct gccggcattg caacggcctc
     5041 aagcgcaacc gcacgccgga agaggcgggc atccagctcc gctacgcgcc ctacgtgccg
     5101 aacaaggccg aatacctcat cctgacgaac cgcaagatcc tggccgacca gatggagttc
     5161 ctggtgcagc acgtggcggc caacagccgc gtgttcgatc tggcgacacg gcgcagcgaa
     5221 gatccgctgg cggcctagtg cgccgcgctt catgaccgca gtgcctgccg taccgccggt
     5281 cgcgctcacg cccgcgtact gggcgcgcgc caagcgcgag ttggcgcgca acgatccgct
     5341 gctcgacgcc gtcctccgcg cccatcccgg cgccgtcctg cgcagccgcg gcgatgcctt
     5401 tcaaaccctg atgcgttcca tcgtcggtca gcagatttcg ctcaaggcgg ccgacagcgt
     5461 gtgggcgcgc ctttgcctgg ctgtggcgcc gcgaccggcg gacgtcatcg ccctggggca
     5521 cgagcggctg gcggcctgcg gactgtcgca acgcaaagcc gagtacatcc tcgatctggc
     5581 gcggcacttc gccgaaggcc gcctgcatat ccaccgctgg ccggaaatgg acgacgaggc
     5641 ggtcatccag gaactgatcg ccgtgcgcgg catcggtcgc tggacggcgg aaatgtttct
     5701 gatgttcaac ctgctgcggc cggacgtgct gcccgtagac gatctgggcc tgcgccgtgc
     5761 ggtaagcgaa ctttatttcg gcggcgagcc gatcggcgcc gacgccgtgc gccatgtggc
     5821 gcgcgactgg gcgccctggc gctcggtggc gacctggtat ctgtggcgca gtctggagcc
     5881 ggtgccggtg gagtattgag acggtcgggc gccggccccg ggaggggccg gttcgtcggt
     5941 tgggcacgac ttccgttaga atccgcctcc atccccctat gagcacggcg atcggcgtcc
     6001 cacgcttcac cgtgcggcac taatgaaatc gatcttccta gaattcgaac agtctgtcgc
     6061 tgaactagaa gcgaaaatcg aagaactgcg tttcgtgcag gatgattccg cggtcgacat
     6121 ttccgaagaa attgcccgcc tgcagaaaaa gagccagagc ctcaccaagg acatctacgc
     6181 caaactttcg ccgtggcaga cggcgcaggt ggcgcgccat ccacagcgtc cctacaccct
     6241 cgattacgtc aacatgctgt tcaccgattt cgaggaactg catggcgatc gcgcctatgc
     6301 cgacgatccg tccatcgtcg gcgggctggc gcgcttcaac ggccagtcgg tcatggtcat
     6361 cggccaccag aagggccgcg acaccaagga aaagatctac cgcaacttcg gcatgccgcg
     6421 gcccgagggc tatcgcaagg cgctgcgcct catgcgcctg gcggaaaaat tcgggttgcc
     6481 ggtgatgacg ttcgtcgaca cacccggcgc ctatcccggt gtcggcgccg aagaacgcgg
     6541 acagtccgaa gccatcggcc gcaacctcta cgtcatggcc gaactcaagg tgccgatcgt
     6601 gagtaccatc atcggcgagg gcggttcggg cggcgcgctg gcgatctcgg tgggcgacgt
     6661 ggtcatgatg ctgcagtact ccacctactc ggtcatttcg cccgaaggct gcgcctccat
     6721 cctgtggaag agcgccgaac gcgcggccga cgcggccgag acgctcggca tcaccgccga
     6781 ccgcctcaag tcgctggggc tgatcgatcg cgtcattccg gagccgctgg gcggtgcgca
     6841 tcgcgattac gccaccgcgg gacagaatct gcgcaaggcc ctgcaggacg cgttgcgcga
     6901 gctgagcaat cacagcccgg cggaactggt ggagcggcgc ttcgagcggc tcatggccta
     6961 tggcaagtac aaggaaaacg cggggaagtg agcttctccc cgtcgtcgaa gccgcactcg
     7021 cggcgcatgt cccgccgggc gcgcggctga cgctcggtta ctcgggcggc gtcgattccg
     7081 ccgcggcgct ggacgcactg gcgcggctgg cggcgccgcg cgaatacacc ctcgactgtc
     7141 tgcacgtcca tcacgggctc agccccaatg cggatcgctg ggccgagttt gcgcagcgca
     7201 cagcggccgg ctacggcatc gcctgtcgcg ttcgcaaggt gaatcccgcc gattatccgg
     7261 ggctcggtgt ggaaggcgcg gcgcgcgcgg cgcgctatgc cgcctacgcc gagtgcgaca
     7321 ccgatttcat cgtgcttgcc caccatcgcg acgaccaggc ggagacgctg ctgctgcagc
     7381 ttgcgcgcgg cgcgggcgtg caggggctgg cggcgatgcc ggtgctgcgg ccgctggagg
     7441 gcgcgcgatc gcagttgttg cgcccgctgc tcgacgtgtc gcgcgcggcg attgccgcct
     7501 atgccgccgg gcgcgatctc gaatgggtgg aggacgagag caatctcgac cgcggctacg
     7561 cgcgcaaccg gctgcggcac gaggtgctgc cggcgctgca ggccatcaat ccggccgccg
     7621 tcgccaacat cgcgcgggcg gccgcgcact tgagcgatgt cgcgcaactg gtcgatgcct
     7681 gggcgggtga ggatctgatg cgtgtcatgc gcgaggatgc ggcggtcgac ctcgtggcct
     7741 tgcgtgcact gggcgaagtc cgcgcgcgtt atgtgttgcg ggcctggctg acggcgcgag
     7801 gtctggtgcc gccgtccatg gccgaattga gtgaagtgct gcgtcagctc gaggatgcgc
     7861 gcgaagacgc cgcgccgcgc ggggatttcg gcgaagtcga attgcggcgt ttccgggacg
     7921 gcgcttatct ggtcgccaag cgtccgccgc caccgcgcga ctatgtcgcg cagtggaccg
     7981 gcgagccgtt gtggccgttg cccgaactgc acggcagcct gcgcttcgat gtcgtgcgcg
     8041 gtgcgggcat cgcgttgcgc catctgccgt ccatgacagt ccgcctgcgg cagggcggcg
     8101 agcgtttccg ccccggaccc ggccggccgc atcgccggct caagcatctg ctgcaggaat
     8161 cggcaatccc gccctggcgg cgcgaacggc tgccgctgct ctatgtcgga agccggttgg
     8221 ccgcggtgcc cggcatcggc atcgatcccg aattcgtcgc cgccgagggc gaaagcggct
     8281 ggctgccgcg ctgggaaacg cagcactgag cggctcccga tgtccgccgc gccgcaatta
     8341 ggctgcggcg cggcaccaaa ggaaggtaaa atcgcgtgct atttcaatgg gaagtcctcc
     8401 gtccttgcgc ggcggggcgc ttccgtgaat gaagcgcatc catttcggcg ggttttgcgc
     8461 ccgccgttca cctcgtaaaa gcactcgtaa aggaagctcg catggcagtc gaacgcaccc
     8521 tgtcgatcgt caaacccgac gcggtcggca agaacgtaat cggaaagatt tactcccgct
     8581 tcgaagacaa tggcctcaag atcgtggcct cgaaaatgaa atggctctcg cgccaggaag
     8641 cggaaggttt ttatgccgtg catcgcgaac gtcccttctt cggtgacctg gtcgatttca
     8701 tggtctccgg cccggtgatg atccaggtcg tggaaggcga gaacgccatt gcgaagaacc
     8761 gtgaactgat gggcgcgacc gatccgaaga aggcggagaa gggcaccatc cgtgcggatt
     8821 tcgccgagag catcgacgcc aacgccgtgc acgggtccga ttccgcggaa aacgccgcaa
     8881 tcgaaatcgc ttatttcttc ccgagcagcg aaatctacag ccgttaattg tccaatccgt
     8941 tcatgagcaa gaatctcctc ggcctcgacg ccgcgcaact gcaggcctat ttcgccgaac
     9001 tgggcgagaa gccgtttcgc gcgcgccagt tgatgcgctg ggtacatcgc aacggcgagg
     9061 ccgactttgc gctcatgagc gatctggcga agtcgctgcg gcagaaactc aacgagtctg
     9121 cggcggtggt gccgccgcgc atgatccagg gcagcgtcgc ggccgacggc acgcgcaagt
     9181 ggctgctcga cgtgggcaac aacaacgcgg tcgaaacggt gtacatcccc gagaccaacc
     9241 gcggcacgct gtgcatctcc tcgcaggccg gttgcaccat ggactgcaag ttctgctcga
     9301 ccggccggca gggcttcaac cgcaacctga ccagcgcaga aatcatcggc cagctatggc
     9361 atgccaaccg cgtgctcggc gccgatgccg acggcgaacg cgtgatcagc aacgtggtga
     9421 tgatgggcat gggcgagccg ctactgaatc tcgataacgt gattccggca ttgaacctga
     9481 tgctcgacga tcacgcctac ggcctgtcgc gccgccgcgt gacggtgtcc acctccggca
     9541 tcgtgccgat gatcgaccgt ttgcgcgacg aatgccccgt tgcgctcgcc gtgtcgctgc
     9601 atgcgcccaa cgacgaattg cgcgatcgca tcatgccgat caaccgcaag tatccgctcg
     9661 ccgaattgct ggccgcgtgt cgccgttacc tggagcacgc gccgcgcgac ttcatcacct
     9721 tcgagtacgt gatgctcgac ggcgtgaacg acagcgtcgc gcacgcgcgt gaattgatcg
     9781 ccttgctggc cgacgtgccg agtaaggtca atctcattcc tttcaatccg ttccccgacg
     9841 ccggcttcgg ccgctcgccg cgggatgtga ttcagcgttt cagcaatctt ttgaatgagg
     9901 caggcatagt gaccacgacc cgcaagaccc gcggcgacga tatcgacgcc gcatgtggcc
     9961 agctggccgg caaggtgcat gaccgtacgc gtcgtgaact gaggatcgcg agggcatcgg
    10021 catgaagcgt ggtgtgctcg tgatcgccct gggtgcgctg ttgagcgccg gctgtcagac
    10081 ggtgcccgag gcgcagcagg atcagatcca ggcggcgcgc gaaattctcg aagcgggcga
    10141 tatgatcgtg gcactgccgg cggacgcgca gacgcgtgcc aaggcgcaca ccgatctggc
    10201 cgcagcgtat tacgaactgg gcaaccttgc cgtcgcgctg gaagaaacgc gtattgccct
    10261 gagagccaat ccgaactatg cgcgcgcgca caacgtgcgc ggattggtac acatggatct
    10321 gcgcgagaac gccgaagcgg aacagagctt caagcgcggt ttgcaactgg cgccgcagga
    10381 cgccgatttg aatcacaact acggctggtt cctctgtcag acgaaccgcg ccgacgaatc
    10441 ggtggagtgg ttcctcaagg cgatcaagaa cccgctctac cgcacgccgg cgcgctccta
    10501 tgcggctgcg ggacattgca cggaacgcag cgacccgaag gaagccgccg aattctacga
    10561 acgcgcattg cgcctcgaac cgaacaaccc gtccgtgctg ctgtccttcg ccggtttgca
    10621 ataccggcag gggcaactgc atgaggcgcg cgcgctcgtg tcgcgctatt acaagctggt
    10681 caaggacccc accgccgaat cgctgtggct tgcgcatcgc atcgaacaca agctcggcga
    10741 tcgcatggcc gccaatcagt acgcgacgga gttgcgtcgc cgttatgcca attccccgca
    10801 gtaccaggct ttgcagcgcg gtcagtacga gtaggaagtc agatggaaga aatgcaggcg
    10861 gaacagaccg ccgcccccca ggaagaagtc ggacccggtc gcatgctggc cgccgcgcgc
    10921 gaggcgcgcg gcgtgtcggt ggacgacgtg gccatgcgca tgaaattcgc gccgcgccag
    10981 atcgaggcgc tggaggctga ccaatacgat cagttgcccg gcgtcgccat cgtgcgcggc
    11041 atggtgcgca actatgcgcg gctgctggag atcgacgccg atccgctgat cgcggaactc
    11101 gaaaagcgca tgcgcaccgg ccccattacc gtgcaacccg cggacatgca cgtgccgatc
    11161 agggaaggcc gcaaggaagg gcgcatgatg ctggtgctgt cgctgattgc gttgatcgcc
    11221 ctcggcgctt tcgtgctcga ctggtatgtg cgcgaccacc gcatcgtcca gccggcccag
    11281 ctggagtcgg acgcggcgga agaggcggcg gaacagacgg acgacaccgt gcagccgctg
    11341 gacgtgcaac ccgcgtcgaa cgccgagccc gccgtctcga ccacgaaagt cgccgcgcct
    11401 gccgcggcga tcatgccggc tgcgcatgtg gtcgaacccg atccggcccc cgctgcggtg
    11461 aaggaggctg atcccgttga cccgccgctc gccgttgcac cgaaagagcc ggagacggaa
    11521 gccgtcgccc ggtccgcgcc cgccgcttcc gacaaggatc tggagatgcg tttcagcgaa
    11581 gacgtctggg tggaagtgaa ggatgcccgc gatcgtctgc tggccgcgcg tctggcgccc
    11641 gcgggcagcg aactggcgct cgacggcgag ccgccgttca aactggtgct gggcaacgcc
    11701 gatgccgtgc agctcaccta caagggcgag ccggtggcga ttccgcagac gcgcagcggc
    11761 gtcgcacgcc tcacactgga gtaggcatga gcgctatgac ccgtcgcctc tcccgcatgg
    11821 tccgcatcgg ccatgtcgcg gtcggcggca ctgcaccgat catggtgcag tccatgacca
    11881 ataccgatac ggccgatgcg gccgcgaccg cgcagcaggt gtacgacctt gcgcaggcgg
    11941 gctcggaagt cgtgcgcatc accgtcaata ccgcggaggc cgccgcgcag gtcgcgaaca
    12001 tccgcgaacg tctcgatgca atgcattgcg acgtgccgct ggtgggcgat ttccatttca
    12061 acggccacaa gctgctgacg cagtatccgg attgcgcgca ggcgctggcg aagtaccgca
    12121 tcaatcccgg caacgtcggc aagggcagca agcgcgacga acagttcgcg accatgatcg
    12181 aactggcctg caagtacgac aagccggtgc gcatcggcgt caactggggc agcctcgatc
    12241 aggatctgct cgcgcgattg atggacgaca acgcccgtcg cgcgcagccg ctcgaagtcg
    12301 atcaggtgat gcgcgaagcg ctcatcgcct cggcgctgga aagcgccgcg cgcgcggagg
    12361 aactgggcct gccgggcgac cgcatcgtgc tgtcgtgcaa ggtgagcgca gtgcaggatc
    12421 tgatcaaggt ctacaccgaa ctggccgcgc gctgcgatta cccgctgcat ctgggcctga
    12481 ccgaggcggg catggggtcg aagggcatcg tcgcctcgac cgctgcgctg gctgtgctgc
    12541 tgcagcaggg cgtcggcgac accatccgca tttcgctcac gcccgaaccg ggcggcgacc
    12601 gcacccgcga agtggtcgtg gcacaggaaa tcctgcagac catgggcctg cgctccttta
    12661 cgccgctggt gatttcctgc cccgggtgcg gacgcaccac cagcacctat tttcaggaac
    12721 tggcggcaaa gatccagcac tacctgcgca cgcagatgcc ggtctggcgt gccgactatc
    12781 ccggcgttga agacatgcat gtcgcggtga tgggctgcgt ggtgaacgga ccgggcgaat
    12841 cgaagcaggc gaacatcggc atcagcctgc ccggtgcggg tgagacgccg gtcgcgccgg
    12901 tgtatgtgga cgggcagaag acggtcacgc tgaagggcgc gcatatcgcc gaagaattcc
    12961 agcagatcgt cgaggattat gtgcacagaa cctacggtcc cggcggccgc gggcgcacaa
    13021 cccggcagaa cgataaaatc atcgcgatcc gcgcggcgca ctgagcgccg cgccgcgttt
    13081 caatccgagg gcgcccggcg ccgcaactat gagtcaaacg ctacaagctg tacgcgggat
    13141 gaacgacatc ctgcccgacg aagccgtgct gtgggagcgc ttcgaagacc ttatccgctc
    13201 ctggctggcg agctacggtt atcgcaacct gcgcacgccg ctgctcgaac atactgcgct
    13261 gttccgccgc gccatcggcg aggcgacgga catcgtcgag aaggagatgt acagcttcgt
    13321 cgacgaattg aacggcgagc acctcaccct gcgcccggaa ggtacggccg ccgcggtgcg
    13381 tgcggtcgtg cagcattcga tgctgtacaa cggcccgcag cggctctggt acatgggtcc
    13441 gatgttccgc cacgagcgtc cgcaaaaggg ccgctatcgg cagttccatc aggtgggcgt
    13501 ggaagcgctg ggctttgccg gcccggacat cgacgccgaa ctgctgctca tgtgcgcgcg
    13561 gctgtgggac gacctgggat tgcagcacat caggctgcag ctcaattcgc tcggcagcag
    13621 cgaggaacgc gcgaagcatc gggaggcgct ggtcgcctat ctcaaatcgc gcgaaagcga
    13681 actggacgag gattccaggc gtcgcctgca taccaacccc ttgcgcgtgc tcgacagcaa
    13741 gaatccggcg atgcagaccc tgatcgccga ggccccgaag ctgctcgact acctcggtgc
    13801 cgaatcgatg gcgcacttcg agggcgtgca gcgcgtgctg aaggacgcgg gcatcgagta
    13861 tgaaatcaat ccgcggctgg tgcgcggtct ggactactac aacctcaccg tgttcgagtg
    13921 ggtgaccgac cggctcggcg cgcagggcac cgtctgtgcc ggcggccgtt acgacggcct
    13981 gatcgcggaa atcggcggca agccggcccc ggcggccggt ttcgccatgg ggatcgagcg
    14041 gctgctcgcg ctgttgtgcg aatccggcga gaatgccgcc gattggctgc agcccacgcc
    14101 gcaggatgtg tacatcgtcc atcagggcga gcaggcgggg cggctcgcct tcaccgtcgc
    14161 cgaggcgctg cgcagtctgg gcttcaccgt gacgcagcat tgcggcggcg gcagcttcaa
    14221 gtcgcaaatg aaaaaggccg acggcagcgg cgcgcatatc gccgtggtca tcggcgacga
    14281 tgaggccgcg gcgcatgaag tgagtgtgaa accgctgcgc gaagaacgcg gtcaatggcg
    14341 cgtgagcgtt gaacaactgc cggaggcgat caccgatctg ctgtacggcg aacaggggga
    14401 gtgagcggtc gtggcgacct acgatctgga agaacaggaa aaaatcgaag aactgaaggc
    14461 gtggtggaag cagtacggca cgttgatcat caccacgacc gcgctggcgc tgatcatcat
    14521 cgccgggatg cgcttctggg acggttacca gcgcacgcag tcggtcgagg ccaatgcggt
    14581 gtacaaccgc ctgctgcagg cggtgcaggc ggatgagggg tcgaaggtcg ccgaactgag
    14641 cgccctggtg cgcgacgatt attcgcgcac cgtttacgcc tcgctcggca cctttgccgc
    14701 ggcgaaatac catgtcgaaa aaggcgagct ggaagcggcg cgcgagcagt tggcatgggc
    14761 cgcggagaac gcccgcgatg cgcagttgaa ggcggtcgcg cgtctgcgtc tggcgcaggt
    14821 gctgatcgat ctgcagcgct acgacgaagc gttgcagcaa ctggaggccg agcatccggc
    14881 ggcgtttacc acgcagtacg cgcagacccg cggcgatatc tacgccctgc aaggcaagac
    14941 tggcgaggcg cgcactgcct atcaggcggc gctggccgcc acgtcgcccg ccgaacgcac
    15001 actgcgcgaa ctgctgcagt tcaagatcga taatctggga gcggtctgat gcgcaagcct
    15061 ctacgcatgc ttggccgcgg cgcggccggg ctcacgctgg tcgtggcgct cggcggttgc
    15121 gcggcgctca atcccttcaa ctggttcggc ggcagtgacg aggcgcgcaa gcccgttccg
    15181 ctgacggaga tttcccaatc gctgcaggtg cgcacgcttt ggcaggcgcg cgtcgggtcg
    15241 agcaagggct ttacgctcgc gccggcggtt gcgcgcggca gtgtctatgc cgccgcttat
    15301 gacggcacgg tggtgcgcct ggaggaggag accggcaagg aagtctggcg tatcgacgtc
    15361 ggcgatggcc tgaccggcgg cgtcggcagc gacggcgaac tggtggtggt cggcacgcgc
    15421 gaaggcgtgg tgatcgcgct cgacaacgac ggccgcgtac tgtggcgcgc gcgcgtgtcg
    15481 agcgaagttt tggtcgcgcc ggtcgtcgcc ggcgatgtcg tcgtggtgcg cagtgccgac
    15541 agccgcctgt tcgcactcga tgcgcgcgac ggcaagcgtc agtgggtgta ccagcgttcg
    15601 gcgccggcgc tgaccgt
//
LOCUS       DATGOU010000081         6590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_1542920, whole genome shotgun sequence.
ACCESSION   DATGOU010000081 DATGOU010000000
VERSION     DATGOU010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6590
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1542920"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VKZ48_05030"
     CDS             <1..510
                     /locus_tag="VKZ48_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLU77250.1"
                     /translation="VAMRVVDIQLSFPAILIALILIAVLGQGVEKVIIALIAVQWAYY
                     ARTARSAAVVEGRKEYVEAAVCQGLRAGRIVFRHLLPNCLPPLTVVATTQIAAAITLE
                     ATLSFLGLGLPITEPSLGLLIAKGYNYLLSGYYWISVFPGLALLAAIVSINLVADRLR
                     DMLDPRLAR"
     gene            582..1760
                     /gene="argE"
                     /locus_tag="VKZ48_05035"
     CDS             582..1760
                     /gene="argE"
                     /locus_tag="VKZ48_05035"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HLU77251.1"
                     /translation="MQAEPVLPIRTETWDMIQRLIAFDTTSRESNLGLIEWVRDWLER
                     QGVESRLTYDATGKKANLFATVGGGSRPGIVLSGHTDVVPVDGQAWDTDPFKATLDGE
                     RIYARGSVDMKSFIGVALAHTPKFLAANLDAPVHFALSYDEEVGCIGVRGLLEDLVRN
                     DIRPAGCIVGEPTGMQPVIAHKGMRSFKCRVHGKEAHSSLTTQGVNAIEYAAKLILFV
                     RQLAERMRDLEARDYNFDVPFTTLQTGLIKGGTAPNIVPRECQFHFEFRYLPGVDPDA
                     LEAEIRDYARNVLEPEMRKVDASVGIEIDTKSQVPGLSTTEAEEIAKLAQRLARAERL
                     KKVAYATEAGLFQQAGIPSIICGPGSIEQAHKPNEFITLEQVALCESFMDRLTATLSR
                     "
     gene            complement(1846..2238)
                     /gene="rplS"
                     /locus_tag="VKZ48_05040"
     CDS             complement(1846..2238)
                     /gene="rplS"
                     /locus_tag="VKZ48_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HLU77252.1"
                     /translation="MNIIEQIEQEEIARLGKNIPDFGPGDTVVVNVNVVEGERRRVQA
                     YEGVVIAKRNRGLNSAFTVRKISSGEGVERTFQTYSPLIASVEVKRRGDVRRAKLYYL
                     RERSGKSARIKEKLVRRNAPAKAAAAAE"
     gene            complement(2296..3054)
                     /gene="trmD"
                     /locus_tag="VKZ48_05045"
     CDS             complement(2296..3054)
                     /gene="trmD"
                     /locus_tag="VKZ48_05045"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HLU77253.1"
                     /translation="MLQFDCITLFPQMFDAVAEYGITRRAREEGKWSLTLWNPRDFTT
                     DNYRTVDDRPYGGGPGMVMLAEPLEKAIRAARARVPDVPARVIYLSPQGAKLDHAKVI
                     ELTQLPRLVLLCGRYEAVDERLIRRCVDEELSLGDFVLSGGEIAAMAVIDAMVRQLPG
                     VLNDAQSAMQDSFVHGLLDCPHYTRPEVYEGEAVPPVLLSGHHADIERWRLKQALGRT
                     WLRRPDLLERRTLSAEEQKLLAEFRAELQTGREA"
     gene            complement(3054..3581)
                     /gene="rimM"
                     /locus_tag="VKZ48_05050"
     CDS             complement(3054..3581)
                     /gene="rimM"
                     /locus_tag="VKZ48_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006577008.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HLU77254.1"
                     /translation="MAERSRSTSTDDMVVMGRVVGAYGVKGWIKVQPYTESIENLCDY
                     ESWWIGRAGEWVEREVLDAAVHGATVLAQLEGLADREAAALLKGSEIAVPRAALPPPE
                     ENEHYWADLIGLAVVNVQGEALGRVAGHVHNGAHDVMRVEHDGGERLIPYVEAIVQEV
                     DLAGRRIVVDWGVDW"
     gene            complement(3600..3857)
                     /gene="rpsP"
                     /locus_tag="VKZ48_05055"
     CDS             complement(3600..3857)
                     /gene="rpsP"
                     /locus_tag="VKZ48_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008342343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HLU77255.1"
                     /translation="MVVIRLARGGAKKRPFFNIVAADSRFASTGRFIERVGFYDPKAP
                     EGREGLRVNGERLAYWQSQGAQVSPTVARLVKQARKTTAAA"
     gene            complement(4033..5388)
                     /gene="ffh"
                     /locus_tag="VKZ48_05060"
     CDS             complement(4033..5388)
                     /gene="ffh"
                     /locus_tag="VKZ48_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HLU77256.1"
                     /translation="MFDNLTQRLSRVVKTIRGEARLTEENIADAMREVRMALLEADVA
                     LPVVKDFIANVREKALGEEVIGSLTPGQALVGVVHRELTALMGGDTATLNLNVAPPAV
                     VLMAGLQGAGKTTTVGKLAKLLKEESRKKPLLVSCDVYRPAAIEQLKTLAQQVGVDFY
                     PSEAEQQPVDIARAAVDHARRHYNDVVLVDTAGRLGIDEAMMKEIAALHEALQPAETL
                     FVVDAMQGQDAVNVAKAFGEALPLTGVVLTKLDGDARGGAALSVRQVTGQPIKFAGVG
                     EKLSGLEPFHPERMASRILGMGDILSLVEEARKGVDEQEAQKLAEKFKKGKGFDLEDF
                     KSQILQMKKMGGLSTLMDKLPAQFAQAAAQTPQSMDDKALGRIEGIINSMTPRERSKP
                     EIIKASRKRRIAAGAGVPVQEVNRLLKQFEQMQKMMKQMQKGGMQKMMRSMKGLLPGM
                     R"
     gene            5448..6305
                     /gene="ccsA"
                     /locus_tag="VKZ48_05065"
     CDS             5448..6305
                     /gene="ccsA"
                     /locus_tag="VKZ48_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HLU77257.1"
                     /translation="MGSHILLHTLTAALYLGLGVYYWRAHWRDGVREVRDRGHYLILI
                     PVVLHTWLLWSELLQAPTFRFGFGQALSVMLWLTVVIYWVESRLFNVQGMQAIILPAA
                     ALCVFVPAIFPGAATPGYTQHLAFRAHLLLAMAAYSLFTIGAMHALLMAALDRTLHGD
                     SAVSGRDSDRAKALLLRGAFAGMPPLLTLERLLFRILGIGFLLLTLTLASGILFSEEL
                     FHQPLQLNHKTIFAFMSWLIFGALLFGRWRYGWRGRRALRWTVSGFAVLLLTYVGTRF
                     VLEVLLARI"
     gene            6374..>6590
                     /locus_tag="VKZ48_05070"
     CDS             6374..>6590
                     /locus_tag="VKZ48_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HLU77258.1"
                     /translation="MDDISLTRQFAALAVLLILSGLFSIAETSMMALNRYRLKAMVRL
                     GNRAAKRTADLLARTDRLLGVVLLGNNL"
BASE COUNT         1090 a         2240 c         1976 g         1284 t
ORIGIN      
        1 gtggcgatgc gtgtcgtgga catccagctt tcctttcccg ccatactcat cgcactcatt
       61 ctcatcgcgg tgctcggcca gggtgtggag aaggtgatca tcgcgctcat cgccgtgcag
      121 tgggcgtatt acgcgcgcac ggcgcgctcg gccgccgtcg tagaaggacg caaggaatac
      181 gtcgaagcgg ccgtgtgcca gggcctgcgc gccggccgca tcgtattccg tcatctgctg
      241 cccaactgcc tgccgccgct caccgtcgtg gcgaccacgc agatcgccgc ggccattacg
      301 ctggaggcga ccctttcctt cctcggtctc ggcctgccga tcaccgaacc ctcgctcggg
      361 ttgctgatcg ccaagggcta caactatctg ctctccggct attactggat cagcgtgttt
      421 ccgggacttg cgctgctggc tgccatcgtc agcatcaatc tcgtcgcgga ccggttgcgc
      481 gacatgctgg acccgcgtct cgcgcgttga atgcgccgac cgggcggtta ggcatggcga
      541 tcggtgtaag atcgtttttt cgctccagcc cctcttccgc aatgcaagcc gaacccgttc
      601 tgccgatccg caccgagacc tgggacatga tccagcgtct catcgccttc gacaccacga
      661 gtcgcgaatc gaatctgggc ctcatcgaat gggtgcgcga ctggctcgag cgccagggcg
      721 tggaatcgcg cctgacctac gacgccacgg gcaagaaagc gaatttgttc gccaccgtcg
      781 gcggcggctc gcgcccgggc atcgtgctgt cgggccacac cgatgtggtg ccggtcgatg
      841 ggcaggcgtg ggacaccgac cccttcaagg caacactaga cggcgaacgt atttacgcgc
      901 gcggcagcgt cgacatgaag agtttcatcg gcgtcgcgct ggcgcatacg ccgaaatttc
      961 tcgcggcgaa cctcgacgca ccggtgcatt tcgcactgtc ctacgacgag gaagtgggct
     1021 gcatcggcgt gcgcgggttg ctggaggatc tggtgcgcaa cgacatccgc ccggccggct
     1081 gcatcgtcgg cgagccgacc ggcatgcagc cggtcattgc gcacaaaggt atgcgcagct
     1141 tcaaatgccg cgtgcacggc aaggaggcgc attcctcgct caccacgcag ggcgtcaacg
     1201 ccatcgaata cgcggcaaag ctgattctgt tcgtgcgcca actggccgag cgcatgcgcg
     1261 atctggaagc gcgcgattac aatttcgatg tgcctttcac caccctgcag acggggctga
     1321 tcaagggcgg cacggcgccg aacatcgtgc cgcgcgaatg ccagttccat ttcgagttcc
     1381 gctacctgcc gggcgtggac ccagatgcgt tggaagcgga aatccgcgac tacgcccgga
     1441 acgtgctcga accggaaatg cgcaaggtgg atgcatcggt gggcatcgag atcgacacca
     1501 aatcgcaggt gcccggtctt tccacgaccg aagcggagga gatcgccaag cttgcgcaac
     1561 ggcttgcgcg cgccgagcgc ctgaagaaag tggcctacgc caccgaagcc ggcctgttcc
     1621 agcaggcggg cattccctcg atcatttgcg gaccgggttc gatcgaacag gcgcacaagc
     1681 ccaacgagtt catcaccctc gaacaggtcg cgctgtgcga atccttcatg gaccgcctga
     1741 ccgcaacgct gagccgctga atcgctgttt agagcttcgc tttaacacca aaccaaaaag
     1801 gacgccgcag cgtccttttc agtcggtgaa gtatcgtgcc gtttcttatt cggcagcggc
     1861 ggccgccttg gccggtgcat tgcggcgcac cagcttttcc ttgatgcgcg cggatttgcc
     1921 ggaacgttcg cgcaggtagt acagcttggc gcgacgcacg tcgccgcgac gcttcacttc
     1981 gacggaagcg atgagcggcg aataggtctg gaaggtccgc tcgacgccct cgcccgagga
     2041 aatcttgcgc accgtgaaag cggaattcag gccgcggttg cgcttggcga tgaccacgcc
     2101 ttcataggcc tgcacgcgcc ggcgctcgcc ttcgaccacg ttgacgttga ccaccaccgt
     2161 gtcgccgggc ccgaagtccg gaatgttctt gcccagacgg gcgatttctt cctgctcgat
     2221 ttgttcaatg atgttcattg ctcgttcgct ccttcaccgt gcaaggagcc ccgctccccg
     2281 ctcatcaaca cttcgtcagg cctcgcggcc cgtctgcagc tccgcgcgga attccgcgag
     2341 cagcttctgt tcttccgcac tcagcgtgcg gcgctccaac aaatccggcc gcctcagcca
     2401 cgtgcgaccc agtgcctgtt tcagccgcca tcgctcgatg tcggcatggt ggccggacaa
     2461 caacaccggc ggcaccgctt cgccttcgta cacttccggc cgcgtgtaat gcggacagtc
     2521 cagcaggccg tgcacgaacg agtcctgcat cgccgattgc gcatcgttca gcacgcccgg
     2581 caactgccgc accatcgcgt cgatcaccgc catcgcggcg atctcgccgc cggacagtac
     2641 gaaatcgccc agcgacaact cttcatcgac gcaacggcgg atcagccgtt catccaccgc
     2701 ttcgtagcgc ccgcacagca gcaccaagcg cggcaactgc gtcagctcga tcactttcgc
     2761 atgatccagc ttcgcgccct gcggcgagag gtagatcacc ctcgccggta cgtccggcac
     2821 gcgcgcccgg gctgcgcgaa tggccttttc cagcggttcg gccagcatca ccatgccggg
     2881 gccgccgccg tacggccggt catcgaccgt ccggtaattg tccgtcgtga agtcgcgcgg
     2941 gttccacagc gtcagactcc acttgccttc ttccctggcc cgacgcgtga tgccgtattc
     3001 ggcaaccgcg tcgaacatct gcgggaacag cgtgatgcag tcgaactgca gcatcaccag
     3061 tccacgcccc agtcgacgac gatgcgccga ccggcaagat ccacttcctg cacaatcgct
     3121 tcgacgtagg gaatcagtcg ctccccgccg tcgtgctcga cgcgcatgac atcgtgcgcg
     3181 ccgttatgga cgtgcccggc cacccggccg agcgcctcgc cctgcacatt caccactgcc
     3241 agaccgatca gatcggccca gtagtgctcg ttctcttccg ggggcggtaa cgccgcccgc
     3301 ggcaccgcga tctcgctgcc cttgagcaag gcagccgctt cgcgatcagc gagtccttcg
     3361 agctgcgcca gcacggttgc gccgtgcact gccgcatcca ggacttcgcg ttccacccat
     3421 tcacccgcgc ggccgatcca ccaggattcg tagtcgcaca ggttttcaat cgattcggta
     3481 tagggctgaa ccttgatcca ccccttcacg ccataggcgc cgaccacgcg ccccatcacc
     3541 accatgtcgt cggtcgacgt gctgcggctt cgttcagcca cgacgtctgc cggcaatgat
     3601 caggcagcag cggtggtttt gcgggcttgc ttgaccaggc gcgccaccgt cgggctcacc
     3661 tgcgcgccct ggctttgcca gtacgccagg cgctcgccgt ttacgcgcag cccttcacgg
     3721 ccttccggcg ccttcggatc atagaaaccg acgcgctcga tgaagcggcc cgtgctggcg
     3781 aagcgcgaat ccgccgctac gatgttgaaa aacgggcgct ttttggcgcc gccgcgagca
     3841 agacgtataa cgaccatgcg tagctactcc gggaaccgcc gaaccatttc ggcagccatc
     3901 gaaaaaagac cgcgattgta cgggagaggg ccgggaatcg gcaagcgacg gcgcacaaaa
     3961 gcctgaaata cggcggaaat tcgccgaaac cggctccgac cgcaggcgta aaggtggccg
     4021 accgccaccg gactaacgca tgccgggcag taagcccttc atcgagcgca tcattttctg
     4081 catgccgccc ttctgcatct gcttcatcat tttctgcatc tgctcgaatt gcttcagcag
     4141 gcgattcacc tcctgcaccg gcacgccggc ccccgcggcg atgcggcgct tgcgcgaggc
     4201 cttgatgatt tcgggcttgc tgcgctcgcg cggcgtcatg gaattgatga tgccttcgat
     4261 ccggccaagc gccttgtcgt ccatggattg cggcgtctgg gcggcggcct gggcaaactg
     4321 cgcaggcagt ttgtccatga gcgtagacag gccgcccatt ttcttcattt gcagaatctg
     4381 gctcttgaag tcctccagat cgaaaccctt gcccttcttg aacttctccg ccagcttctg
     4441 cgcttcctgc tcgtcgacac ctttgcgcgc ctcttccacc agcgagagga tgtcgcccat
     4501 gccgagaatg cgcgaagcca tgcgctccgg gtggaagggc tcgaggccgc tcaacttttc
     4561 gcccacgccg gcgaatttga tcggctgacc ggtgacctgc cgcaccgaca gggctgcgcc
     4621 gccgcgggca tcaccgtcga gcttggtgag taccacgccg gtgagcggca gcgcttcgcc
     4681 gaaggccttg gcgacgttga cggcgtcctg accctgcatc gcatcgacga cgaagagcgt
     4741 ctcggccggc tgcagcgctt cgtgcagtgc ggcgatttcc ttcatcatcg cctcgtcgat
     4801 gcccaggcgg cccgccgtat cgaccagcac cacatcgtta tagtgtctgc gcgcgtgatc
     4861 cactgccgcg cgcgcgatat ccaccggctg ctgctcggcc tccgagggat agaaatccac
     4921 cccgacctgc tgggccagcg tcttcaactg ctcgatggcg gcgggacggt aaacgtcgca
     4981 ggacacgagc agcggcttct tgcgcgactc ttccttcagc agtttggcca gcttgccgac
     5041 cgtcgttgtc ttgcctgcgc cctgcagacc ggccatcagc accacggccg gcggcgccac
     5101 gttgaggttc agggtggccg tgtcgccgcc catcagggcg gtcagttcgc gatggaccac
     5161 gccgaccagc gcctgcccgg gggtcaggct gccgatcacc tcctcgccga gcgccttctc
     5221 ccgtacgttg gcaatgaaat cctttaccac cggcagggcc acatcggcct cgagcagcgc
     5281 catccgcact tcgcgcatgg cgtcggcgat gttttcttcg gtgaggcggg cttcgccgcg
     5341 gatcgtcttg acgacgcgcg aaagccgctg ggtcaggttg tcgaacatgg tattcctgcg
     5401 atacgaagcg ctgccgtcgg ggcggagcga tgggataaac tgccgcgatg ggaagccaca
     5461 ttctactgca cacgctgacc gctgccctgt atctgggcct cggcgtctat tactggcgcg
     5521 cgcattggcg ggacggcgtg cgggaggtgc gcgaccgggg ccactacctg atcctgatcc
     5581 ccgtcgtcct gcacacatgg ctgctgtgga gcgaactgct gcaggcaccc accttccgct
     5641 tcggtttcgg tcaggcgctg tcggtgatgc tgtggctgac cgtcgtcatc tactgggtgg
     5701 aaagccgtct gttcaatgta cagggcatgc aggcgatcat tctgcccgcc gcggcgctgt
     5761 gcgtgttcgt gccggcaatc tttcctggcg cggcgacgcc cggctataca cagcatctgg
     5821 ctttccgcgc gcatctgctg ttggcgatgg ctgcctacag cctgtttacc atcggtgcca
     5881 tgcatgcgct gctcatggcc gcgctcgacc gcacgctgca cggcgattcc gccgtatcgg
     5941 gccgcgacag cgaccgcgcc aaagcgctgc tcctgcgcgg cgcatttgcc gggatgccgc
     6001 cgttgctcac gctggagcgg ttgctgttcc gcatcctggg catcggcttc ctgctgctca
     6061 cgctcacgct cgccagcggc attctgtttt ccgaggaact gttccatcag ccgttgcaac
     6121 tgaaccacaa gacgatcttt gcgttcatgt cctggctgat tttcggggcc ctgctgttcg
     6181 gccgttggcg ctacggctgg cgcgggcggc gcgccctgcg ctggacggtg tcgggcttcg
     6241 ccgtgctgct gctgacctat gtgggcacgc gcttcgtgct ggaagtactg ctcgcgcgga
     6301 tctgagccgc ggttgacagc ggcgcacgcc cccgccgata ctttttcgcg tttttcctcc
     6361 acccgctcca gatttggacg acatctcgct aacacggcaa ttcgccgcgc tagccgttct
     6421 gctgattctt tccggcctgt tctccatcgc cgaaacgagc atgatggcgc tcaaccgcta
     6481 tcggctgaag gcgatggtgc gactgggcaa tcgcgccgcc aagcgcacgg ccgatctgct
     6541 cgcccgcacc gatcgtctgc tcggcgtcgt gttgctgggc aacaacctgg
//
LOCUS       DATGOU010000082        17247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3328271, whole genome shotgun sequence.
ACCESSION   DATGOU010000082 DATGOU010000000
VERSION     DATGOU010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17247
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3328271"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1787)
                     /gene="glnE"
                     /locus_tag="VKZ48_05075"
     CDS             complement(<1..1787)
                     /gene="glnE"
                     /locus_tag="VKZ48_05075"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HLU77259.1"
                     /translation="MTTASAATPATAETLREALRHSRYAQRLLAARPQYQAELEAGVH
                     GGWSADAMQAFLRQAGADAKGVTEDALSARLRELRARVMLRLAARDLAGLAPLEEVTG
                     TMTALAEVALAQALDFHRAAMVARHGRPLADGVEQPMLIVGMGKLGGRELNVSSDIDL
                     ILLYPEDGETDGARPLANQEFFVRLGQRIIRMLNDITADGQVFRVDMRLRPWGDAGAL
                     AASFDALEQYFVAHGREWERYAWIKARVVAGGDEASIAALEQLSRPFVFRKYLDYGTI
                     AAVRALHAQIRREVMRRDLGDHIKLGPGGIREIEFIAQSFQLIRGGRDSALRVRPTLA
                     VLQTLGAKHLIEPETLRELTEAYVFLRRLEHRLQYVDDAQTHRLPADEADRALIASSM
                     GFADWPALQAELDRHRAAVTRRFEGVFADTEDEDSRFRPVCDGSLTDDEAAVQLSHCG
                     FQDPHGVMERLRALRNSHRYQSLPGASHARLEALLPRLLEAVCRTATPDAALARCIDF
                     IESISRRAAYLALLQESPQALARVAELLSSSPWAATYLTRHPALLDELLDVRQLHMPS
                     DWSEFLLDLRREMAAHEGDTERQMDLMREL"
     gene            2036..5950
                     /locus_tag="VKZ48_05080"
     CDS             2036..5950
                     /locus_tag="VKZ48_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HLU77260.1"
                     /translation="MSTSEAHREIEQLAPRERAWLRWLRRIGWTLIGVYFIAAASLLV
                     ARYWLVPQLPAYRAAFEEAASEALGQRVSIASFEADWYGFHPWVELRDVTVHDRDGRP
                     ALELPQVRAVIAWRSLPARELRLRMLVIDDADLDIRRTADGRLYVAGLELASSPTASE
                     SSAADWVLRQGEIHVRRARIEWLDEERGAPPLQLSDFNLLVRNSGNTHEFAVRASPPA
                     EYGEALDLRGRLSGRSFAQLRDWNGELYAAIDALDLDAWQQWVDYPVELTGGRGALRM
                     WLTVAQRDLAQLRTRVALTDVRMRVAPELPALELAALQGELGASRRQSTLAMLGLGNG
                     HDIYEAYGRNLHVRPVVGGPLQPIDFTARWEAGEDGEPARGEVRAETVELARLAQLSE
                     HFLLPQGLRTLVQRVQPTGLLQKAEYAWQGPVQTPQSYEARARFSGLGMAPYEDIPGF
                     DGLEGNFSLTERGGRVRAEGRRMGIHYPGVFMVERFAFDRLNARVNWTLRDGGVRVRL
                     EDVQFDNADASGTVTGEVRTTAAGTQWLDLSGRASRARGAAVYKYIPELPTEVGEWLR
                     EGILQGQASDVRFRLKGDLNDFPYSDPRKGEFKVSAKLAGVQLHYADGWPRIDDIVGR
                     IEFDGPRLDIEAARAVSVGAQLHGVRASFADLYADHPVLRVTGNARGATADFLKFIDR
                     SPVREFIGGWTDGWRAKGPGQLQLSLTLPLDDLEHSKVAGSFRFEDNTIVMAPDDPPM
                     TQVNGPVNFSERGASSEGIDARFIGGPLAVRIAQRDDAVVVTAAGSGEAAGLVRLIGL
                     PIGERVRGMTDYHAIFTTRDGRTDSLITTDLRGVDIDLPPPFSKRAAESWPLRVERNA
                     ESATRQRLSARLGTVLAMQGVLRKDGARMVLDRAGVAIGSVAVPRPAGAHIAVAVNVP
                     QLNVDALAPLASGGTEAGVPGMAPLGAIDLRTDALIGAGRIVRNVQLQAQLHDRIWRA
                     DINARELAGTVSWLPQGNGVIKARLRHLYQPETVQGATDALKEYENLPALDVVAERYV
                     LDGRELGRLELQAFNEQGGWRIDHAQLGTPHGTASATGRWRPARSGPERTDLAIAVKA
                     SDLGAYLADFGYVGAIRGGHAELTGNLGWNGPPTELHYATLSGELRVVAEDGQFVQLQ
                     PGVGRLLGVLSLQSLPRRITLDFKDVFGEGFAFDRIDGTATVTRGVMKTDNLGMVGPS
                     ATVLITGTADIARETQNLHVRVVPTVGDSVAAAAGLALLNPVVGVGAWIAQRLLKDPL
                     GRMLAFEYEVTGSWDDPQVRKLGEPEAPAASAQRGGQGNEAQ"
     gene            5947..6756
                     /locus_tag="VKZ48_05085"
     CDS             5947..6756
                     /locus_tag="VKZ48_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010809059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HLU77261.1"
                     /translation="MTNPYKVAAIQMVSEPGIDANLAMAGELIAEAAQGGAKLVALPE
                     YFCHMGMAETDKVLCCEADGDGPVQAFLAAAAAKHGVWLVGGTVPMRCDDPDRVRNSC
                     MVYDPNGHRVARYDKIHLFGFHNERERYLESATIEAGNQPAAIDTPMGRLALSVCYDI
                     RFPELYRALAPVDLITVPSAFTATTGQAHWELLVRARAVENLAYVLAPAQGGKHLNGR
                     RTHGHSMIVDPWGRILAERSTEGPGVVIAEIDPTYQAKVRRSLPALDHRVL"
     gene            6795..8240
                     /gene="tldD"
                     /locus_tag="VKZ48_05090"
     CDS             6795..8240
                     /gene="tldD"
                     /locus_tag="VKZ48_05090"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HLU77262.1"
                     /translation="MQTDTEVLRAASRTLLAPYDLEESKLARVFGSLMQHKVDYADLY
                     FQYQRSEGWSLEEGIVKSGSFNIDQGVGVRAISGEKTAFAYSDEISLPALEQAANATR
                     AIAGVGGKTKVPSVRAGQGLALYAGLDPIASLADTEKVKLLERLEQLCRARDPRVTQV
                     MASLGAVYEVVLVARSDGMMAADVRPLVRLSVQVIAEQNGRREQGHAGGGGRHDYSLF
                     TDEVLESYAKQAVDQALTNLESRPAPAGTMTVVLGPGWPGILLHEAVGHGLEGDFNRK
                     GSSAFAGRIGQRVASPGVTVVDDGTLAGRRGSLNIDDEGNPTQCTTLIEDGILRGYMQ
                     DSLNARLMKQPVTGNGRRESYAHNVMPRMTNTYMLNGDKHPDEIIASVDRGLYAVNFS
                     GGQVDITNGKFVFAASEAYMIENGKITHPVKGATIIGNGPDALTRVSLIGNDMALDSG
                     IGTCGKEGQSVPVGVGQPTMRIDGLTVGGTA"
     gene            8461..9633
                     /locus_tag="VKZ48_05095"
     CDS             8461..9633
                     /locus_tag="VKZ48_05095"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008451880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLU77263.1"
                     /translation="MLVFDVIVIGAGAAGMMCAATAGQRGRRVLLIDHAAKLGEKIRI
                     SGGGRCNFTNVHCSPERFLSRNPHFCRSALARYTPRDFIALVEKHGIAYHEKKLGQLF
                     CDDSSERIIDMLRAECDAGSVTWRMPCAVEAVARDDEGFLVSTSRGQYRGASLVIATG
                     GLSIPKIGATPFGYRLAEQFGLRIVPPRAGLVPLAFDVNEMKLYGDLAGVSLDVAVSC
                     NGGRFRENLLFTHRGLSGPAILQISSYWQPGDALHIDLLPDASAEALLFEQRRSRALP
                     ATLLSEYWPRRFAQQWCAAVGATQPIDQCNDACLREFAARLHDWTVKPTGSVGYAKAE
                     VTLGGIDTDELSSKTMEAKAVPGLYFIGEVVDVTGHLGGFNFQWAWASGHAAGQAL"
     gene            complement(9635..10081)
                     /locus_tag="VKZ48_05100"
     CDS             complement(9635..10081)
                     /locus_tag="VKZ48_05100"
                     /inference="COORDINATES: protein motif:HMM:NF037928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HLU77264.1"
                     /translation="MQTTWIVAADASRARIFRMLGRNQIEEIEDFIHKESRQKDRELR
                     TDAVGQFSVRGAMPGGDMVERTDPTDHEAEIFSRTIGSYLEKARTQNKYDQLCLIAPP
                     KFLGLLRKNLSKEAQRMVDKEVPKDISWFNPRDLESYIRSTRELPD"
     gene            complement(10325..10906)
                     /locus_tag="VKZ48_05105"
     CDS             complement(10325..10906)
                     /locus_tag="VKZ48_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HLU77265.1"
                     /translation="MGTITPDSLMTLEAYAKIRKSSRQQAIAHRWLRTVRVGDYISVQ
                     FEDEQTIRRQIQEMLHIEKIFDEEGIQDEIDAYQPLVPDGSNWKATMLIEYPDVEQRK
                     RELARLIGIEDRMYVEVQGHARVYAIADEDLERETEDKTSAVHFLRFEFNAAQCDAIR
                     SGAPVTLGCDHPNYTASTVIAPEKLASLASDLR"
     gene            complement(10918..12270)
                     /locus_tag="VKZ48_05110"
     CDS             complement(10918..12270)
                     /locus_tag="VKZ48_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HLU77266.1"
                     /translation="MNTREGSLEAPTRHPIDWQSAEFYDEASCFTELERIFDICHGCR
                     RCVSLCQAFPTLFDLVDETEDGELHGVAKADYWKVIDQCYLCDLCYMTKCPYVPPHPW
                     NLDFPHTMLRAKAIKFKNGGVKAGERFLASTDVHGKFAGIPIVVQTVNAVNKTKPARK
                     LMDRTLGVHPEAWMPELATRRFRSGAAHKGSASEVVNGERTPGKVAIYSTCYVNYNEP
                     GIGHDLVKLLEHNGIPYEIVEKESCCGMPKLELGDLDTVAKHKERNMPVLARYAREGW
                     AIVTPVPSCTLMYKQELPLMFPDCADTQAVKEAMFDPFEYFVARHKDGLLKTDFKQAL
                     GKVSYHVPCHGRVQNIGRKTEEMFKLIGQSVEVKLNTVERCSGHAGTYGVKTPTHPVA
                     MKIGRPVFRAMAKDEPDYISSDCALAGHHIAQGMEQNNLKQAKLEHPLSLMRRAYGLE
                     "
     gene            complement(12371..12790)
                     /locus_tag="VKZ48_05115"
     CDS             complement(12371..12790)
                     /locus_tag="VKZ48_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HLU77267.1"
                     /translation="MELKGSKTEQNLKDAFAGESQANRRYLYFANKADVEGQNDIAAL
                     FRSTAEGETGHAHGHLEFLEAVGDPATGLPIGDTRANLKAAVAGETHEYTDMYPGMAK
                     TAREEGFDEIADWFETLAKAERSHANRYQKALDTMVD"
     gene            12960..14177
                     /locus_tag="VKZ48_05120"
     CDS             12960..14177
                     /locus_tag="VKZ48_05120"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HLU77268.1"
                     /translation="MGAPSPWSGPIETHRYRRCLAGACAALGLCASFASAEETSGWDF
                     GGALRFNYVQNDYASGYDGAGKLDFDTARVNLDYRRDALTGSFEYRYYRGRAAGDGRF
                     VQSVFDGDATDSHFLHHAWVGWRFANDDEVRLGVHKVPFGALPFASHSYFFQLPYYVG
                     LEDAYNLGIGYAATRGDWRAHLAFYARPGPDGHGESRDSARYTYNLVETESADNRERN
                     TVVGRLARVLRHRADASTEVGLSLLAGEVPDGVSGRTGHRWAAALHAQGTYGRWGLKA
                     EWIRYRFDLKSAETDVVMMGAYDAPYEVASQGDLLVAGLSYRLPVGRGPLDEVSLYYD
                     YSVLYKDVAGFPHSRQSIVGAAWTLGKWLIYTDLTFGKHHPFIGPDYGAALGPGGDDE
                     WHRRFNINVGYYF"
     gene            complement(14305..16326)
                     /locus_tag="VKZ48_05125"
     CDS             complement(14305..16326)
                     /locus_tag="VKZ48_05125"
                     /inference="COORDINATES: protein motif:HMM:NF014126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HLU77269.1"
                     /translation="MTQSADPSESQYTADLNKPVFYISAGFVVVLVLLTALFTERMSA
                     LLGALQRGIVEHFGWYYILVVAGFLFFILALLFSRYGSIRLGDDDEEPEYSYPTWFAM
                     MFAAGMGIGLLFYGVAEPILHFIEPLESEPSSVPAAREAMELAYFHWGLHAWGVYIVV
                     GLSMAYFAYRHSLPLTIRSTLYPLLGERAYGGIGNAVEIIALMGTLFGVATSLGLGAA
                     QINSGLAHLNLMPVSTANQLWLIVGIALMATASVASGLDRGIRRLSEANLIMATLLLL
                     FVFLAGPTVFVLSTFVQNLGHYVQTLIEMSFRTTAYRGIEWQASWTMFYWGWWISWSP
                     FVGMFIARISRGRTLREFIAGALFAPTLVGFIWFAVFGNTALHMELFGEGGIAEAVQA
                     NVPTALFVMLDKLPFATITTTLATVLVVFFFVTSSDSGSLVMDILAHNGNPNPPVGTR
                     IFWGLSQGVIAAVLLLAGGLAALQTAALASALPFSVVMIFMCISLVMGLQAEALTSHV
                     GPASTRLLPQNGDGSATDDDTPPDWRTQLRQLIGRRPAVRQEESEVTACRDSIRAFIR
                     QADEAFQNLATELARNGRETIIDRHGYQIGMTVLRNGREEFTYTLRGRTSRRAAFAFP
                     QLSHPETRARCQLDIILRGGVVEEARLEDFDQDRIYQHFLTEYAKWVGW"
     gene            complement(16403..>17247)
                     /locus_tag="VKZ48_05130"
     CDS             complement(16403..>17247)
                     /locus_tag="VKZ48_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HLU77270.1"
                     /translation="GVLAHTAADLYGAAIPIAGIAGDQQAALYGQACLRPGMAKNTYG
                     TGCFMLLHTGAECISSQNGLISSRAGIAAGSPPEYCLEGSVFIAGAVVQWLRDQLGVI
                     RAAPEVEALAASVEDSDGVYFVPAFAGLGAPHWDPHARGALFGVTRGTSSAHIARAAL
                     ESVAFQSADVLLAMEQDAGLQLAELRVDGGATANNLMMQFQADLLGVPVVRPVIQETT
                     ALGAAYLAGLATSVWRSADEVEALWQREREFIPAMSRDEAQNRLAAWRRAVECARGWT
                     SDRN"
BASE COUNT         2927 a         5481 c         5843 g         2996 t
ORIGIN      
        1 agttcgcgca tcaggtccat ctgtcgttcg gtatcgccct cgtgcgccgc catttcgcgc
       61 cgcagatcga gcaggaactc gctccagtcg gacggcatgt gcagttgccg cacgtcgagc
      121 agttcgtcga gcagggccgg gtggcgcgtg agataggtcg ccgcccacgg cgaggacgac
      181 aacagctccg ccacgcgcgc cagcgcctgc gggctttcct gcagcagggc gagataggcc
      241 gctcggcggc tgatggattc gatgaaatcg atgcagcgcg ccagtgccgc atccggcgtg
      301 gcggtgcggc acaccgcctc cagcaggcgc ggcagcagcg cctcgaggcg cgcgtggctt
      361 gcgccgggca gggattgata gcgatggctg ttgcgcaggg cacgcaggcg ctccatcacg
      421 ccgtgcggat cctggaagcc gcaatgggaa agctgcaccg ccgcttcatc gtcggtcagg
      481 ctgccgtcgc acaccggccg gaagcggctg tcctcgtcct cggtatcggc gaacacgcct
      541 tcgaagcgcc gcgtcacggc ggcgcgatga cggtcgagtt ccgcctgcaa tgccggccag
      601 tcggcgaagc ccatgctcga tgcgatgagc gcgcgatcgg cctcgtcggc gggcaggcgg
      661 tgcgtctgcg cgtcgtcgac atattgcagc cgatgctcca gccggcgcag gaagacgtac
      721 gcttcggtca attcccgcaa cgtctcgggt tcgatcagat gctttgcccc cagcgtctgc
      781 agcacggcca gggtggggcg tacgcgtaac gccgaatcgc gtccgccgcg gatcaactgg
      841 aaggactgtg cgatgaattc gatttcgcgg atgccgcccg gtccgagctt gatgtggtcg
      901 cccagatcgc ggcgcatgac ttcgcggcga atctgcgcat gcagcgcgcg caccgcggcg
      961 atggtgccgt agtccagata cttgcggaac acgaaggggc gcgacaactg ctccagcgcg
     1021 gcaatgctcg cttcgtcgcc gccggccacc acgcgcgcct tgatccaggc atagcgctcc
     1081 cactcgcggc cgtgggcgac gaaatactgt tccagcgcgt cgaaactggc ggccagcgcg
     1141 cccgcatcgc cccagggtcg cagccgcatg tcgacgcgga acacctgccc gtcggcggtg
     1201 atgtcattca gcatgcggat gatgcgctgc cccaggcgca cgaagaattc ctgattcgcc
     1261 agcggccggg cgccgtcggt ctcgccgtcc tcgggataga gcaggatcag gtcgatgtcc
     1321 gacgagacgt tcagttcacg tccgcccagc ttgcccatgc cgacgatcag catcggctgt
     1381 tccacgccgt cggccagcgg gcggccgtgg cgcgccacca tggccgcgcg atggaaatcg
     1441 agcgcctgcg caagcgcaac ctcggccagt gccgtcatcg tgccggtcac ttcctccagc
     1501 ggcgccagac cggccaggtc gcgcgctgcc agccgcagca tgacccgcgc gcgcaattcg
     1561 cgcagtcgcg ccgacagggc gtcttccgtc acgcccttcg catccgcgcc cgcttggcgc
     1621 aggaacgcct gcatcgcatc ggcactccaa ccgccgtgca ccccggcttc cagttccgcc
     1681 tggtattgcg gccgcgccgc cagcagtcgc tgcgcgtagc gcgaatggcg cagcgcctcg
     1741 cgtagcgttt cggcggttgc aggggtggcg gcggatgctg ttgtcatggg cgggaaggtg
     1801 cgcgcggcag tatagaatcg ccgggttgcc gaaagcttcg gcgctcgtgt cgcgggcagc
     1861 gaccgccgca ttgtaaagcg tgtgaagcgg ccggcgggcg cgtgcgccga gcgcaagcga
     1921 atagtgagga aagccatcga agcaacattc aacccggcgc accgcatgag gcgccggttc
     1981 cacccgggcg gcccgcgccg cctttcgacc acccgttgcc cggatcacgt ccatcttgtc
     2041 cacgtctgaa gcccatcgcg agatcgagca actggcgccg cgcgagcgcg cctggctgcg
     2101 gtggttgcgg cgcatcggct ggacgctgat cggcgtctat ttcatcgccg cggccagctt
     2161 gctggtcgcg cgctactggc tggtgccgca gttgccggcc taccgcgccg ccttcgagga
     2221 ggcggcctcg gaggcgctgg gccagcgcgt ttccatcgcc tccttcgaag cggattggta
     2281 cggcttccat ccctgggtgg aattgcgcga tgtcaccgtg cacgaccgcg acggtcgtcc
     2341 ggcgctggag ctgccgcagg tgcgtgcggt catcgcctgg cgttcgctgc cggcgcgcga
     2401 actgcggctg cgcatgctgg tgatcgacga tgcggatctg gatattcgcc gcactgcgga
     2461 cggacggttg tacgttgccg gcctggagct cgcctcgtcg ccgaccgcct ccgagtcgag
     2521 cgcggccgac tgggtactgc ggcagggcga aatccatgtc cgccgcgcgc gcatcgaatg
     2581 gctggacgaa gaacgcggtg cgccgccgct gcaactgagc gacttcaatc tgctcgtgcg
     2641 caacagcggc aacacgcacg agttcgccgt gcgcgcgtcc ccgccggccg agtacggcga
     2701 ggcgctggat ctgcgcggcc ggctgtccgg ccgcagtttt gcgcaattgc gcgactggaa
     2761 cggcgagctc tacgccgcca tcgacgcgct cgatctggat gcgtggcagc agtgggtcga
     2821 ctatcccgtc gagctgacgg gcggccgcgg cgccctgcgc atgtggctca ccgtggcgca
     2881 gcgcgacctg gcgcaactgc gcacgcgcgt cgccctgaca gacgtgcgga tgcgcgtggc
     2941 gccggaactg ccggcgctgg aactggccgc tctgcagggc gaactcggcg cgagccgccg
     3001 ccagagcacg ctggcgatgc tggggctggg caacggtcac gacatctacg aggcctacgg
     3061 ccgcaatctg catgtgcgtc cggtcgtcgg cgggccgctg cagcccatcg acttcaccgc
     3121 ccgctgggaa gcgggcgagg acggcgagcc ggcgcgcggc gaagtgcgcg cggagacggt
     3181 ggaactggca cgccttgcgc aattgagcga acactttctc ctgccgcagg gactgcgcac
     3241 actggtccag cgcgtgcagc ccaccgggct gctgcagaaa gcggagtacg cgtggcaggg
     3301 gccggtgcag acgccgcaaa gctacgaggc gcgtgcgcgc ttcagcgggc tgggcatggc
     3361 cccctatgag gacattcccg gcttcgacgg gctggagggc aacttctcgc tcaccgagcg
     3421 cggtggacgc gtgcgggccg aagggcgccg catgggcatt cactatcccg gcgtgttcat
     3481 ggtggagcgc ttcgccttcg accggctcaa cgcgcgggtg aactggacgc tgcgcgatgg
     3541 cggcgtgcgt gtgcggctgg aggatgtgca gttcgacaac gccgacgcga gcgggacggt
     3601 gacgggcgaa gtgcgcacca cggcggccgg cacgcagtgg ctggacttga gcgggcgcgc
     3661 ttcgcgcgcg cgcggcgcag ccgtttacaa atacatcccg gaactgccga cggaagtggg
     3721 cgagtggctg cgcgagggca tcctgcaggg gcaggcgagc gacgtgcgct tccgcctcaa
     3781 gggcgatctg aacgacttcc cctacagcga tccgcgcaag ggcgagttca aggtgagcgc
     3841 caagcttgcc ggcgtgcaat tgcattacgc tgacggctgg ccgcgcatcg acgacatcgt
     3901 cgggcgcatc gagttcgacg ggccgcgtct ggatatcgag gccgcgcgcg ccgtctccgt
     3961 cggcgcgcaa ctgcacgggg ttcgcgcaag cttcgccgat ctgtacgccg accatcccgt
     4021 gctgcgggtg acgggcaacg cacgcggcgc caccgccgat ttcctcaagt tcatcgatcg
     4081 cagtccggtg cgcgagttca tcggcggatg gacggacggc tggcgggcga aggggccggg
     4141 gcagttgcaa ctgagcctga cgctgccgct ggatgatctg gagcacagca aggtcgcggg
     4201 cagcttccgc ttcgaggaca acacgatcgt catggcgccg gacgaccccc ccatgacgca
     4261 ggtgaacggg ccggtgaatt tcagcgaacg cggcgcgtcg agcgaaggca tcgatgcgcg
     4321 cttcatcggc gggccgctgg cggtgcgcat cgcccagcgc gacgatgccg tcgtcgtcac
     4381 ggctgcggga tcgggcgagg cggccggact ggtacgcctg attgggctgc ccatcggcga
     4441 acgcgtccgc ggcatgaccg actaccacgc catcttcacc acgcgcgacg gccgcaccga
     4501 cagcctgatc accaccgatc tgcgcggcgt cgacatcgac ctgccgccgc cgttcagcaa
     4561 acgcgccgcg gaaagctggc cgttgcgcgt cgaacgaaac gccgaaagcg ctacgcgaca
     4621 gcgtttgagc gcgcgcctgg gcaccgtgct ggcgatgcaa ggcgtgctgc gcaaggacgg
     4681 cgcgcgcatg gtgctggacc gcgccggcgt ggcgatcggc agcgtcgcgg taccgcgtcc
     4741 ggcgggggcg catatcgccg tcgccgtgaa cgtgccgcaa ctgaacgtgg atgcgcttgc
     4801 gccgctggcg agcggcggga ccgaagcggg cgtgcccggc atggcgcccc tcggtgcaat
     4861 cgatctgcgt accgatgcgc tgatcggcgc cggacgcatc gtgcgcaacg tgcagttgca
     4921 ggcgcaattg cacgaccgca tctggcgcgc ggacatcaat gcgcgcgaac tggccggcac
     4981 cgtgagctgg cttccgcagg gcaacggcgt gatcaaagcg cgcctgcggc atctttatca
     5041 gcccgagacc gtgcagggcg cgaccgacgc gctgaaggaa tacgagaacc tgcccgcgct
     5101 ggatgtggtc gccgagcgct atgtgctgga cggacgcgaa ctcggccgac tggagttgca
     5161 ggccttcaac gagcagggcg gctggcgtat cgatcacgcg caactgggta cgccgcacgg
     5221 tacggcctcg gccaccggcc gctggcgacc ggcacggagc gggccggagc gcaccgatct
     5281 ggcgatcgcc gtcaaggcga gcgatctcgg cgcctatctt gcggatttcg gctatgtcgg
     5341 cgcgatccgg ggcggacacg cggagttgac gggcaacctc ggctggaacg gcccgcccac
     5401 cgaactgcat tacgccacgc tctccggcga actgcgcgtc gtggccgagg acggccagtt
     5461 cgtgcagttg cagccgggcg tgggacggtt gctgggcgtg ctcagtctgc agtcgctgcc
     5521 gcggcgcatc acgctggact tcaaggacgt gttcggcgag ggtttcgctt tcgatcgcat
     5581 cgacggtacg gcgactgtga cgcgcggtgt gatgaaaacc gataatctcg gcatggtcgg
     5641 gccgtcggcc accgtgctca tcaccggcac ggcggatatc gcgcgcgaaa cgcagaacct
     5701 gcacgtgcgg gtcgtgccca cggtgggcga cagcgtcgcc gccgcagcgg ggctggcgct
     5761 gctcaatccg gtggtcggcg taggtgcgtg gatcgcgcag cgtctgctga aggatccgct
     5821 cggccgcatg ctggccttcg aatacgaagt gaccgggagt tgggacgatc cgcaggtacg
     5881 caagctgggt gagcccgagg cgccggcggc atccgcgcaa cgcggcggac aggggaacga
     5941 agcgcaatga cgaatccgta caaggtcgcc gccatccaga tggtgagcga gccgggcatc
     6001 gatgcgaatc tcgccatggc gggtgagttg atcgccgagg cggcgcaggg aggcgcaaag
     6061 ctcgtcgcgc tgccggagta tttctgccac atgggcatgg ccgagaccga caaggtgctg
     6121 tgctgcgaag cggacggcga cggtccggtg caggcctttc tggccgccgc cgcggcgaag
     6181 cacggcgtct ggctggtggg cggcaccgtg ccgatgcgct gcgacgatcc ggatcgcgtg
     6241 cgcaacagtt gcatggtgta cgaccccaac ggccaccgcg tcgcgcgcta cgacaagatc
     6301 cacctgttcg gcttccacaa cgagcgcgaa cgctatctgg aatctgcgac catcgaggcc
     6361 ggcaaccagc ccgccgcgat cgacacaccg atgggcaggc tggcgctttc cgtgtgttac
     6421 gacatccgct ttcccgagct ttaccgtgcg ctcgcgccgg ttgatctgat caccgtgccg
     6481 tcggcgttca ccgccacgac gggtcaggcg cactgggaac tgctggtgcg tgcgcgggca
     6541 gtggagaacc tcgcgtacgt gctcgcgccg gcccagggcg gcaagcatct gaacgggcgc
     6601 cgcacacacg gccacagcat gatcgtggat ccctggggcc gcatcctcgc cgagcgcagc
     6661 acagaaggac cgggcgttgt aatcgccgaa atcgacccga cttatcaggc caaggtgcgc
     6721 cgcagcctgc ctgcccttga tcaccgcgtg ctctagacgg cacgccgcaa cactgaacaa
     6781 cggattattc gctcatgcaa accgataccg aagtcctccg cgccgcgagc cgtaccctgc
     6841 tcgcccccta tgatctggaa gaaagcaagc tcgcgcgggt attcggctcg ttgatgcagc
     6901 acaaggtcga ttacgccgat ctttatttcc agtatcagcg cagcgaaggc tggagcctgg
     6961 aagaaggcat cgtcaaatcg ggcagcttca atatcgatca gggcgtgggg gtgcgcgcca
     7021 tcagcggcga gaagaccgct ttcgcctact cggacgagat cagcctgccc gcgctggagc
     7081 aggcggccaa tgccacgcgc gccattgccg gcgtcggcgg caagaccaag gtgccgagcg
     7141 tacgggccgg acaggggctg gcgctctatg ccggactcga tcccattgcc tcgctggccg
     7201 acaccgagaa agtgaagctg ttggagcgcc tggagcagtt gtgtcgggcg cgcgatccgc
     7261 gcgtgacgca agtgatggcg agcctggggg cagtctatga agtggtgctg gtcgcgcgca
     7321 gcgacggcat gatggctgcg gatgtgcggc cgctggtgcg gctgtcggtc caggtgatcg
     7381 ccgaacagaa cggccggcgc gaacagggcc atgccggcgg cggggggcga cacgactact
     7441 cgctgttcac cgacgaagtg ctggaaagct atgccaaaca ggcggtggat caggcactga
     7501 ccaatctgga gtcgcgtccc gcaccggcgg gcacgatgac cgtcgtgctc ggccccggct
     7561 ggccgggcat cctgctgcac gaggcagtgg gtcacggtct ggaaggcgac ttcaatcgca
     7621 aaggttcgtc ggcgttcgcg gggcggatcg gccaacgtgt ggcatcgccg ggcgtgaccg
     7681 tcgtcgacga cggcacgctc gccgggcggc gcggttcgct caacatcgac gacgaaggca
     7741 atccgacgca gtgcaccacg ctgatcgagg acggcatcct gcgcggctac atgcaggaca
     7801 gcctcaatgc gcgcctgatg aagcagccgg tgacgggcaa cggccggcgc gagtcgtatg
     7861 cgcacaacgt gatgccgcgg atgaccaaca cctacatgct gaacggcgac aaacacccgg
     7921 acgagatcat cgcctcggtc gatcgcggtc tttatgccgt caacttctcc ggcgggcagg
     7981 tggacatcac caacggcaag ttcgtgttcg ccgcctccga agcctacatg atcgagaacg
     8041 gcaagatcac ccacccggtg aagggcgcga ccatcatcgg caacggcccc gacgcgctca
     8101 cgcgggtctc cctgatcggc aacgacatgg cgctcgacag cggcatcggc acctgcggca
     8161 aggaagggca gagcgtgccg gtcggcgtcg gccagccgac gatgcgtatc gacgggctga
     8221 cggtcggcgg cacggcgtga agatcaaaat cttcaaccac caagacacca agacaccaag
     8281 aagggcttaa aacctgaacc acagaggcac agagacgcag agacacagag aaaagcagga
     8341 tcttcgacgc caagatgctc gcggcattgt cgttcttggc gtttccttgg cgccttcgtg
     8401 ccttggtggt aaagcttttt attcggttgt cctctgtgcc tctgtgtctc tgtggtgaag
     8461 atcttggttt ttgatgtaat cgtcatcggc gccggtgcgg ccggcatgat gtgcgccgcc
     8521 accgcggggc agcgcggccg acgcgtactg ctcatcgatc atgccgccaa gctgggcgag
     8581 aaaatccgta tctccggcgg tggtcgctgc aatttcacca atgtgcattg cagtccggag
     8641 cgctttctgt cgcgcaatcc gcacttctgc cgttcggcgc tggcgcgcta cacgccgcgc
     8701 gacttcatcg cgctggtcga gaagcacggt atcgcctatc acgagaagaa actggggcaa
     8761 ctgttctgcg acgacagcag cgaacgcatc atcgatatgc tgcgcgcgga gtgcgatgcg
     8821 ggcagcgtca cctggcgcat gccctgtgcg gtggaggcgg tcgcacgcga cgacgaaggc
     8881 ttcctcgtca gcacttcacg agggcaatat cgcggcgcat cgctggtcat cgcgaccggc
     8941 ggactgtcga ttccaaaaat cggcgcgacg ccgttcggct atcggctggc cgagcaattc
     9001 ggtctgcgca tcgtgccgcc gcgtgccggt cttgtgccgc tcgcgttcga cgtcaacgag
     9061 atgaagctgt atggcgatct cgccggcgtc tcgctcgacg tcgccgtgtc gtgcaacggc
     9121 ggtcgcttcc gtgaaaatct gctgttcacc catcgcggct tgtcgggccc cgccattttg
     9181 cagatttctt cctattggca gccgggtgat gccttgcata tcgacttgct gcccgacgcg
     9241 tcggctgaag cgttgctgtt cgagcagcgg cgcagtcgcg cgttgccggc aacattgttg
     9301 agcgagtatt ggccgcgtcg cttcgcacag cagtggtgcg ccgcggtggg cgcaacacag
     9361 cccatcgatc aatgcaacga tgcctgcctg cgcgagttcg ccgcacgcct gcacgactgg
     9421 acggtcaagc cgaccggcag cgtgggctac gccaaggcgg aagtgacgct gggcggcatc
     9481 gatacggatg agttgtcctc caagaccatg gaggcgaagg cggtgcccgg cctatatttc
     9541 atcggcgaag tggtcgatgt gaccggacac ctgggcggct tcaattttca gtgggcatgg
     9601 gcgtcgggcc atgccgccgg acaggcgctt tgactcagtc cggcagttcg cgggtgctgc
     9661 ggatgtagct ctcgaggtcc cgcggattga accaggagat gtccttgggg acttccttgt
     9721 ccaccatgcg ctgggcctcc ttgctcagat tcttgcgcag cagccccaga aacttcggcg
     9781 gagcgatgag acagagctgg tcgtacttgt tctgggtgcg cgccttttcc aggtagctgc
     9841 cgatcgtacg gctgaagatt tccgcctcgt gatcggtggg gtcggtccgc tcgaccatgt
     9901 cgccgcccgg catcgcaccc cgcacggaaa actgcccgac ggcgtcggtg cgcagctcac
     9961 gatccttctg tcggctctcc ttgtggatga agtcttcgat ttcctcgatc tgattgcggc
    10021 cgagcatccg gaagatgcgt gcgcggctcg cgtccgcggc gacaatccag gtggtttgca
    10081 tgaggcgtac tccttcgctg gcgtatgcgc agttggggga gggccgatca gatgtgggcg
    10141 acgagcgcgc gttgccgcat gcgagcgcgg gctgaactcg ctacggaaac aagcagtatc
    10201 gaacctcctc acggcggcgt cgcaacctgc gtgcccgtgg cgcggcgacc ctcttcagtc
    10261 tgtccaggaa gaaaaacgcc gaacgcgggc gaggcgttcg gcgtcgtacg ggggcattcg
    10321 ccggtcagcg caagtcggag gccagactgg cgagtttttc cggcgcgatg acggtggatg
    10381 cggtgtagtt gggatggtcg cagcccagcg tcaccggtgc gccggagcgg atggcgtcgc
    10441 attgagcggc attgaattcg aagcgcagga agtgcaccgc ggacgttttg tcctcggtct
    10501 cgcgctccag gtcttcgtcg gcgatggcat agacccgtgc gtggccttgc acctcgacgt
    10561 acatgcgatc ctcgatgccg atcaggcgcg ccagttcgcg tttgcgctgt tccacgtccg
    10621 gatactcgat cagcatggtg gccttccagt tgctgccgtc cggcaccagc ggttgatacg
    10681 catcgatctc gtcctgtatg ccttcctcgt cgaaaatctt ttcgatgtgc agcatctcct
    10741 ggatctggcg gcggatggtc tgctcgtctt cgaactgcac actgatgtag tcgccgacgc
    10801 gcacggtacg cagccagcga tgtgcgatcg cctgctggcg actgctcttg cggatcttgg
    10861 cgtaggcctc caatgtcatg aggctgtccg gtgtgatcgt gcccatgagg tctcctttca
    10921 ttccaggccg taggcgcggc gcatcaaact gagcggatgc tcgagtttgg cctgcttcag
    10981 attgttctgt tccatgccct gcgcgatgtg gtgtccggcc agcgcgcagt cggaactgat
    11041 gtagtccggt tcgtccttcg ccatggcgcg gaacaccggc cggccgattt tcatcgccac
    11101 ggggtgagtc ggcgtcttca cgccgtaggt gccggcgtgg ccggagcagc gttcgacggt
    11161 gttgagcttc acctcgacgc tctgaccgat gagcttgaac atctcctcgg tcttgcggcc
    11221 gatgttctgc acgcggccgt gacagggcac gtgatagctc accttgccca gcgcctgctt
    11281 gaagtcggtt ttcagcagac cgtccttgtg gcgcgcgacg aagtattcga agggatcgaa
    11341 catcgcttcc ttcaccgcct gcgtgtcggc acagtcgggg aacatcagcg gcagttcctg
    11401 cttgtacatc aacgtacagg aaggcacggg cgtcacgatc gcccagccct cgcgcgcata
    11461 gcgcgccagg accggcatgt tcctttcctt gtgtttggcc accgtgtcca gatcgcccag
    11521 ttcgagcttc ggcatgccgc agcagctttc cttttcgacg atctcgtacg ggatcccgtt
    11581 gtgctcgagc agcttgacga ggtcgtgtcc gatgcccggc tcgttgtaat tgacgtaaca
    11641 agtggagtag atcgccacct tgcccggcgt gcgctcgccg ttcaccactt ccgaagcgct
    11701 ccctttgtgt gccgcgccgg agcggaagcg cctggtcgcc agttccggca tccaggcctc
    11761 agggtgcacg cccagtgtgc gatccatcag cttgcgcgcg ggcttggtct tgttcactgc
    11821 gttgaccgtc tgcacgacga tgggaatgcc cgcgaacttg ccgtgcacgt ccgtggaggc
    11881 gaggaagcgt tcgcccgcct tgaccccgcc gtttttgaat ttgatcgcct tggcgcgcag
    11941 catggtgtgc gggaaatcga gattccacgg gtgcggcggc acgtagggac acttggtcat
    12001 gtagcacagg tcgcacaggt agcactggtc gatcaccttc cagtagtcgg ccttggcgac
    12061 gccgtgcaat tcaccgtctt cggtctcgtc gaccaggtcg aacagcgtag ggaaggcctg
    12121 gcacagactc acgcagcggc gacacccgtg acagatgtcg aagatgcgct ccagttccgt
    12181 gaaacaggac gcctcgtcat agaactcggc gctctgccag tcgatgggat gtctcgtcgg
    12241 cgcttcgagg ctgccttctc gtgtattcat gttgttgctc ctcctgctcc cggtccgcct
    12301 gaccggcccg caaaaacgaa gggggcgtgc acgccccctt cgctgatggc gactacatcg
    12361 atgtgccgac tcagtcgacc atcgtatcga gcgctttctg atagcggttg gcgtgcgagc
    12421 gctcggcctt ggccagggtt tcgaaccagt cggcgatttc gtcgaagcct tcctcgcgcg
    12481 cggtcttggc catgcccggg tacatgtcgg tgtactcgtg cgtttcgccg gcaacggcgg
    12541 ccttcaggtt ggcgcgcgtg tcgccgatcg gcaggccggt ggccggatcg ccgacggctt
    12601 ccaggaattc cagatggccg tgcgcatggc ccgtttcgcc ttcggcggtg gaacggaaca
    12661 aagcggcgat gtcgttctga ccctcgacgt ccgccttgtt agcgaaataa agatagcggc
    12721 ggttggcctg ggattcgccg gcgaaggcgt ccttcaggtt ctgctcggtc ttcgaacctt
    12781 tcagttccat gactgggtcc tcctcgaata gaagtgggat atacagccgt ccgcgacgcg
    12841 gactaagcca gacgaaccta gcccaggatg cggccggggg caatacaaac tgtcaatcgg
    12901 gccgattggc ttaaggcatc gggctcgagg gtgccgcggc aggccctccc gctgcatact
    12961 tgggcgcacc gagcccctgg agcggtccga tcgaaaccca ccgatatagg cgatgtctgg
    13021 ccggcgcatg cgccgcatta gggctgtgcg catccttcgc gtcggccgag gaaacgtccg
    13081 gctgggattt cggcggtgcg ctgcgcttca attacgtcca gaacgattac gcctcgggtt
    13141 acgacggcgc cggcaaactg gatttcgata cggcgcgggt caacctcgac taccgtcggg
    13201 acgcgctgac cggttcgttc gaatatcgct actaccgggg gcgcgcggcc ggcgacgggc
    13261 gcttcgtgca atcggtcttc gacggcgacg ccaccgacag tcattttctg caccatgcgt
    13321 gggtcggctg gcgattcgca aacgacgacg aagtgcggct tggcgtccac aaggtgccgt
    13381 tcggcgcgct gcccttcgcc agccattcct acttcttcca actgccctat tacgtggggc
    13441 tcgaagacgc ctacaacctg ggcatcgggt atgcggccac gcgcggcgac tggcgcgcgc
    13501 acctggcgtt ctacgcgcgc ccgggtcccg acggtcacgg cgaaagccgc gacagcgcgc
    13561 gctacaccta caacctggtg gaaaccgaaa gtgccgacaa tcgggagcgc aacaccgtcg
    13621 tcgggcgtct ggcgcgcgtg ctgcggcacc gcgcggacgc ctcgaccgag gtcggtctgt
    13681 cgctcctggc gggcgaagtg ccggacggcg tgagcggccg caccggccat cgctgggcgg
    13741 ccgcattaca tgcccaaggc acctacggcc gttggggttt gaaggccgaa tggattcgtt
    13801 accgcttcga tctgaagagc gcagagaccg atgtggtcat gatgggtgcc tacgacgcgc
    13861 cttacgaagt cgcttcccaa ggcgatctgc tggtggcggg gttgagctac cggctgccgg
    13921 ttgggcgtgg cccgctggac gaggtctcgc tctattacga ctacagcgtc ctctacaagg
    13981 atgtcgccgg ctttccgcac agccgccaga gcatcgtcgg cgccgcctgg acgctgggca
    14041 aatggctgat ttacaccgac ctgacgttcg gcaaacatca tcctttcatc ggcccggatt
    14101 acggcgccgc actcgggccg ggtggtgacg acgaatggca ccgccgcttc aacatcaacg
    14161 tcggctatta cttctagcct ttaaaacccg gaacacagag aagaacggag gacatgtagt
    14221 aagcattaag cgggagtttc tctgcgctct atgccgtctg tggcgaagga ttttccatct
    14281 gcaccgcgca agcgcttgcc gcactcacca gccgacccac ttggcgtatt cggtgaggaa
    14341 gtgctgatag atccggtcct ggtcgaaatc ctccagccta gcttcctcca ccacaccgcc
    14401 gcgcaggatg atgtcgagtt ggcagcgggc acgggtttcc gggtgggaca attgcgggaa
    14461 tgcgaacgcg gcgcgacgtg aggtgcgtcc gcgcagcgta taggtgaact cctcgcggcc
    14521 gttacgcagc accgtcatgc cgatctgata gccgtggcgg tcgatgatcg tttcgcgacc
    14581 gttgcgtgcg agttccgtgg cgaggttctg gaacgcctcg tccgcctgcc ggatgaatgc
    14641 acgaatgcta tcccgacagg ccgtgacttc cgactcttcc tgcctcacgg ccggccgccg
    14701 gccgatgagc tggcgtagtt gcgtgcgcca gtcgggcgga gtgtcgtcgt cggtggcact
    14761 cccgtcgccg ttctgaggca ggagacgagt cgatgccggg ccgacgtgcg aggtcaacgc
    14821 ttccgcctgc aggcccatta caagactgat gcacatgaag atcatcacca cgctgaaggg
    14881 cagcgccgag gcgagggcgg ccgtctgcag cgccgccagg ccgccggcga gcagcagcac
    14941 ggcggcgatg accccctggg agagccccca gaagatgcgc gtgccgacgg gtggattcgg
    15001 gttgccgttg tgggcgagga tgtccatcac cagcgatccc gaatccgacg acgtcacgaa
    15061 gaagaacacc accagcactg tcgccagagt cgtcgtgatg gtggcaaacg ggagcttgtc
    15121 gagcatgacg aacagggcgg tcggcacatt ggcctgcacg gcctcggcga tgccgccttc
    15181 gccgaacagt tccatgtgca gcgcggtgtt gccgaatacg gcgaaccaga tgaaaccgac
    15241 cagggtcggt gcgaacagcg cgccggcgat gaattcgcgt aaggtccggc cgcgcgaaat
    15301 gcgggcgatg aacatgccga cgaagggaga ccaggagatc caccaacccc agtagaacat
    15361 ggtccagctc gcctgccatt cgatgccccg gtaagccgtg gtgcggaagc tcatctcgat
    15421 cagggtctgc acatagtggc cgaggttctg cacgaaggtg ctgagcacga acaccgtcgg
    15481 cccggcgaga aagacgaaca acagcagcaa agtcgccatg atgagattgg cctcgctcag
    15541 acggcggatg ccccggtcga ggcctgaagc gaccgaggcc gtcgccatta gtgcgatgcc
    15601 gacgatcagc cagagttggt tggcggtcga gaccggcatc agattgaggt gtgcgagccc
    15661 ggagttgatc tgcgcggcgc ccaggccgag cgaagtggcg acaccgaata gcgtccccat
    15721 caaagcgatg atttccaccg cgttgccgat gccgccgtag gcgcgttcgc ccagtagcgg
    15781 atagagcgtg gagcggatgg tgagcggcag cgaatgccgg taggcgaagt aggccatcga
    15841 cagcccgacc acgatataga cgccccaggc gtgcagtccc cagtggaaat aggccagttc
    15901 catggcctcc cgcgcggcgg gcacggagct cggttcgctt tccagcggtt cgatgaagtg
    15961 caggatgggt tcggccaccc cgtagaacag caggccgatc cccatgcctg ccgcgaacat
    16021 catggcgaac caggtagggt agctgtattc cggttcctcg tcgtcgtcgc ccagacggat
    16081 cgaaccgtag cgactgaaga gcagagcgag gatgaaaaac aggaaaccgg cgacgacgag
    16141 gatgtagtac cagccgaagt gctcgacgat gcctctttgc agcgcgccga gcagggcgga
    16201 cattctctcg gtgaacagcg cggtgagcag gacgaggacg acaacgaaac cggccgagat
    16261 gtagaagacg ggcttgttga ggtcagcggt gtactgggat tcggacggat cggcggattg
    16321 cgtcaattcg ggctccccag gagcgatgaa tcgtcgatgg gccggcgcga ccgcgggcgg
    16381 tcgcgccggg gcgtttatgc gcttaattac ggtccgatgt ccaacccctg gcgcactcga
    16441 ctgcacgacg ccaggcggcc aggcgatttt gcgcctcgtc gcggctcatg gcgggaatga
    16501 attcgcgctc tctttgccac aaggcctcca cttcgtccgc cgagcgccaa acgctcgtgg
    16561 cgagcccggc cagataggcc gccccgagcg ctgtcgtttc ctggatgacg ggtcgcacca
    16621 ccgggacgcc gagcaggtca gcctggaatt gcatcatcag attgttggcg gtggcgccgc
    16681 cgtccacccg cagctctgcc agttgcaaac cggcgtcctg ctccatggcc agcagcacgt
    16741 ccgccgactg gaaggcaacg gattccaggg ccgcgcgagc gatatgcgcg ctgctggtgc
    16801 cgcgcgtgac cccgaacagc gcgccgcgcg catgcgggtc ccaatgcggt gccccgaggc
    16861 cggcgaaggc gggcacgaaa tagacgccgt ccgaatcctc gacgcttgcg gcgagggctt
    16921 cgacctccgg cgcggcgcga atgacgccaa gctgatcgcg cagccactgg acgaccgccc
    16981 cggcgatgaa cacgctgcct tcgaggcagt actcgggcgg actgccggcg gcgatgccgg
    17041 cgcgcgaact gatcagaccg ttttgcgagc tgatgcattc cgcgccggta tgcagcagca
    17101 tgaaacagcc ggtgccgtag gtgtttttcg ccatgcccgg ccgcaggcac gcctgaccgt
    17161 agagtgccgc ctgctggtcg cccgcgatgc cggcgatggg aatggcggcg ccgtacagat
    17221 cggcggcggt atgcgcaagc acgccgg
//
LOCUS       DATGOU010000083         5516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_6445597, whole genome shotgun sequence.
ACCESSION   DATGOU010000083 DATGOU010000000
VERSION     DATGOU010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6445597"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1309
                     /gene="ftsA"
                     /locus_tag="VKZ48_05135"
     CDS             71..1309
                     /gene="ftsA"
                     /locus_tag="VKZ48_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HLU77271.1"
                     /translation="MRPANKETKNLIVGLDIGTSKVVALVADVLPDGSLDVIGMGTHD
                     SKGLKKGVVVNIEATVYAIQRALEEAELMADCKIDRVYTGIAGSHIRSFNSTGMVAIK
                     DKEVAPLDVQRVIETARAMPIPTDQQVLHILTQEFVVDGQDGIREPLGMSGVRLEVKV
                     HIVTGAVSAAQNIVKCVRRCGLEVADLILQPLASSIAVLSEDEKELGVCLVDIGGGTC
                     DIAVFREGAIRHTAVIPIAGDQVTSDIAMALRTPTPEAELIKLRYGCALRQLADPNEM
                     VEVPGLGDRGSRQLSRQTLAEVIEPRIEELYTLIQQVLRQSGFEDLLSSGIVLTGGSS
                     VMQGMVELGEEIFHMPVRLGVPRYSGGLAEVVHNPRYATAMGLLLEGKSQFDRGVMSR
                     QTGSFKATLRRMREWFQRNF"
     gene            1494..2648
                     /gene="ftsZ"
                     /locus_tag="VKZ48_05140"
     CDS             1494..2648
                     /gene="ftsZ"
                     /locus_tag="VKZ48_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HLU77272.1"
                     /translation="MFEILDTHQNGTVIKVIGVGGAGGNAVNHMIRNGVGGVDFIAAN
                     TDAQALARSLTKHQIQLGGTGLGAGAKPDQGRAAAMEQREAIREALQGANMCFITAGM
                     GGGTGTGAAPVVAEIAKDMGILTVAVVTKPFAFEGRRMRTAEAGIEELQGNVDSLIVI
                     LNEKLMEVLGDDVSMKDAFRAADDVLHNAVAGIAEIINCPGLVNVDFEDVKTVMSEMG
                     MAMMGSAFAEGVDRARIAAEQAVASPLLEGVNLSGARGVLVNITASEELKMREVNEVM
                     NTVRSFASEEATIIFGAVYDEAMGDDLRVTVVATGLGQEQALRAPKPQLVVAQKTGTD
                     NMAGPGYEYEYPMESAPAVFRKNRSSAVEALSQSGMDKYDIPAFLRKQAD"
     gene            3024..3938
                     /gene="lpxC"
                     /locus_tag="VKZ48_05145"
     CDS             3024..3938
                     /gene="lpxC"
                     /locus_tag="VKZ48_05145"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013958069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HLU77273.1"
                     /translation="MLKQRTLKSAIKATGVGLHGGKKVTMTLRPAAPNTGVVFRRVDL
                     PQPVDIPADALAVTDTRLCSLVEKDGVKVGTVEHIMSALAGLGIDNVYIDLDAPEVPI
                     MDGSASPFVFLLQSAGIEEQRAAKRFFRIKKRVEVQDGDKWARFEPYDGYKLSFSIVF
                     DHPVFEKSSQTVTVDFAETSYAKEVARARTFGFAQDVEALRNHGLALGGSLDNAIVLD
                     EYRVLNSDGLRYADEFVKHKVLDAIGDLYLVGYPIIGAFTAYKSGHGLNNQLLRATLA
                     DATAWELVSFDKAEQAPPALSRLYALPA"
     gene            3969..4154
                     /locus_tag="VKZ48_05150"
     CDS             3969..4154
                     /locus_tag="VKZ48_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77274.1"
                     /translation="MLILRLLAFLVVLTVGASAVVYLLTGNRRYLRFGMQVFKYGLIV
                     GALIVAFIFLERLVLAI"
     gene            complement(4151..4810)
                     /locus_tag="VKZ48_05155"
     CDS             complement(4151..4810)
                     /locus_tag="VKZ48_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLU77275.1"
                     /translation="MYRQQTIGVALIALVVSGCAGMSDTQRHTATGAGIGAAAGAVIG
                     AATAGGNRGRSTATGAAIGAGVGAIGGYIWSTRMEQQKAEMEQATAGTGIEVERTADN
                     RIKVHIPSDAGFDINRSTVKPQLARVLDDFAQGMRTNATTQIHIVGHTDSTGTDAINN
                     PLSRDRAASTRNYLASRGVASSRISIDGRGSHEPIASNATADGRAQNRRVEIYVGEVA
                     R"
     gene            complement(4892..5311)
                     /locus_tag="VKZ48_05160"
     CDS             complement(4892..5311)
                     /locus_tag="VKZ48_05160"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HLU77276.1"
                     /translation="MSGKPLRSFLQTGNELIPQAERLLALRRAVERSLPANLRTAAQV
                     ANLKGDTLILHAGGNAVAAKLKHCTASLVEALAREGYHVASIKVEVQPDCPVPARPRT
                     VRPLPAGAAEGLRRLEQELPADSELRRVIAALAAKPR"
BASE COUNT          977 a         1640 c         1890 g         1009 t
ORIGIN      
        1 gcgctgcgcg tgcccggcat cgagaaattc gaatcgcagc gagggaaggc ggggagtgcc
       61 tgataaaaca atgagaccgg caaacaaaga aaccaagaac ctcatcgtcg ggctggacat
      121 cggcacgtcg aaggttgtcg cgctggttgc ggacgtgctg ccagacggca gcctggacgt
      181 gatcggcatg ggcacgcacg actccaaagg cttgaagaag ggcgtggtgg tcaacatcga
      241 ggcgacggtc tacgccatcc agcgcgcgct ggaagaggcg gaactgatgg ccgactgcaa
      301 gatcgatcgc gtctacaccg gcatcgccgg cagccacatc cgcagcttca actccaccgg
      361 catggtggcg atcaaggaca aggaagtggc gccgctggac gtgcagcgcg tgatcgaaac
      421 cgcgcgcgcc atgccgatcc cgacggacca gcaggtgctg cacatcctga cgcaggaatt
      481 cgtcgttgat ggacaggacg gcatccgcga gccgctgggc atgagcggcg tgcggctgga
      541 agtgaaggtg catatcgtca ccggcgcggt atcggcagcg cagaacatcg tcaaatgcgt
      601 gcgccgctgc ggcctcgaag tggccgatct cattctgcag ccgctggcgt cgagcatcgc
      661 cgtgctgtcg gaggacgaaa aggaactggg cgtgtgcctg gtcgatatcg gcggcggcac
      721 ctgcgacatt gcagtgttcc gcgaaggcgc catccgccat accgcggtga taccgatcgc
      781 gggcgatcag gtcaccagcg acatcgccat ggcgctgcgc acgccgacac cggaggcgga
      841 actgatcaag ctgcgctatg gctgtgcgct gcgacaactc gccgatccga acgaaatggt
      901 ggaagtgccg ggactgggcg atcgcggctc gcgacaactg tcgcgtcaga cgctggcgga
      961 agtaatcgag ccgcgcatcg aggagctcta cacgctcatc caacaggtac tgcggcagtc
     1021 gggcttcgag gatctgctgt cgtcggggat tgtgctcacg ggcggctcat cggtgatgca
     1081 gggcatggtg gagctgggcg aagagatttt ccacatgccc gtgcgtctcg gggtgccgcg
     1141 ttattcgggc gggctggctg aagtggtgca caacccgcgc tacgccaccg cgatgggatt
     1201 gctgctcgaa ggcaagtcgc agttcgaccg gggcgtgatg tcgcggcaga cgggcagctt
     1261 caaggcgacg ctgcgccgta tgcgggaatg gtttcaaagg aatttctgaa cgggcgcggt
     1321 tgcccgttcg ggaaggggct cggcggaggg ttgcaagacc tgaccgaccg caagctgtgt
     1381 tcggtacgtg aggagtgaag agtgagagga gtgtgaacga tgacggaaca agccactcgt
     1441 ttcacccttc acccttcgcg ggctcggtaa tttgacgatt ggaggtaagg gcaatgtttg
     1501 agattctgga tacgcatcag aacgggaccg tgatcaaggt catcggcgtg ggcggtgccg
     1561 gcggcaacgc cgtcaaccac atgattcgca acggcgtcgg cggcgtggat ttcatcgccg
     1621 cgaacaccga cgcgcaggcg ttggcgcgca gcctgacgaa gcatcagatt cagcttggcg
     1681 gcacgggcct gggtgccggg gccaagcccg atcagggccg cgccgcggcc atggagcagc
     1741 gcgaagccat tcgcgaggcg ctgcagggcg cgaacatgtg cttcatcacc gccggcatgg
     1801 gcggcgggac cggtacgggc gcggcgccag tggtggcgga aattgcgaag gacatgggca
     1861 ttctcaccgt cgccgtcgtg accaaaccct tcgccttcga agggcggcgc atgcgtacgg
     1921 ccgaggcggg catcgaggaa ctgcagggga acgtcgattc gctcatcgtc atcctcaacg
     1981 aaaagctgat ggaggtgctg ggcgacgatg tcagcatgaa ggacgccttc cgcgcagcgg
     2041 acgacgtact gcacaacgcc gtggcgggca tcgccgaaat catcaactgc ccgggcctgg
     2101 ttaacgtcga cttcgaagac gtgaagacgg tcatgagcga aatgggcatg gcgatgatgg
     2161 gctccgcctt tgccgaaggc gtggaccgcg cgcgtattgc ggcggaacag gcggtcgcga
     2221 gtccgctgct ggagggcgtc aacctctccg gtgcgcgcgg cgtgctggtg aacatcacgg
     2281 cgagcgaaga gttgaagatg cgcgaggtca acgaagtgat gaacaccgtg cgcagctttg
     2341 cctccgaaga ggcgaccatc attttcggcg cggtctacga cgaagcgatg ggcgacgatc
     2401 tgcgcgtcac cgtggtggcg acgggcctgg gtcaggagca ggcgctgcgc gcccccaaac
     2461 cgcaactggt ggtggcgcag aagaccggga cggacaacat ggcggggccg ggttacgagt
     2521 acgaataccc gatggaatcc gcgccggccg tgtttcgcaa gaatcgcagc tccgccgtcg
     2581 aggccctcag ccagagcggg atggataagt acgacattcc ggctttcctg cggaagcagg
     2641 ccgattaagg cctgcttccc gggccgggat tcatatttac ggcggagact catgcggcct
     2701 gaaaggccgc gtgatgtcgg agccacaatc tggcggccag cactggcgat gcgatccacc
     2761 gaaggggacg gcgtacttgc gtaaacagat ggatgagggg gcgtcgtgga agatgatggg
     2821 gtctccaggt attgcgtgag acgcggcgtc cggcggggag gtgccgccct gctacccgaa
     2881 gatcgcgcgg ggctggccat ggccgcgata tttgcttgaa tgaagtgaga aagtaggcat
     2941 gactgcgggc catcctcccc cgtccgggac atggccgggc ggcttgcgca tgctaaaatc
     3001 cgcgtctttg cgaattgatt gcgatgctga agcagcgaac acttaaatcg gccatcaagg
     3061 ccaccggcgt cgggttgcat gggggcaaga aggtcaccat gaccctgcgc ccggctgcgc
     3121 cgaacaccgg cgtggtgttt cgccgcgtgg atttgccgca gccggtggat attcccgccg
     3181 atgcactcgc tgtcaccgat acccgtcttt gcagcctggt cgaaaaggac ggcgtcaagg
     3241 tcggcaccgt ggagcacatc atgtccgcgc tggcgggctt gggcatcgac aacgtctata
     3301 tcgacctcga cgcacccgaa gtgccgatca tggacggcag cgcaagtcct ttcgtcttcc
     3361 tgctgcaatc ggccgggatc gaagagcagc gtgcagccaa gcgtttcttc cggatcaaga
     3421 agcgcgtcga ggtgcaggac ggcgacaagt gggcgcgctt cgagccctac gacggctaca
     3481 agctgtcctt ctccatcgtc ttcgatcatc cggtgttcga gaaatcgagc cagacggtca
     3541 cggtggattt cgccgagacc tcctatgcca aggaagtcgc tcgggcgcgc accttcggct
     3601 tcgcgcagga cgttgaggcg ctgcgcaatc acggcctggc gctcggcggc agcctcgaca
     3661 acgctatcgt gctggatgag taccgcgtgc tgaacagcga cggcttgcgt tatgccgacg
     3721 aattcgtgaa gcacaaggtg ctggacgcca tcggcgatct ttatctggtc ggctatccga
     3781 tcatcggtgc cttcaccgcc tacaagtccg gccacggcct gaacaaccag ttgcttcgcg
     3841 ccaccctggc cgacgccacg gcgtgggaac tggtcagctt cgacaaggcc gagcaggcgc
     3901 cgccggcatt gagccgcctc tacgcactgc ccgcctgatc agcatcgccc cggcaagacg
     3961 ctgaagcgat gctgatcctc cggttactcg cttttctggt cgttctgacc gtcggcgcat
     4021 cggccgtcgt gtatctgctg accggcaacc ggcgctatct gcgcttcggc atgcaggtct
     4081 tcaagtacgg cctgatcgtc ggcgcgttga tcgtggcgtt catcttcctc gaacgcctgg
     4141 tgcttgcgat ctagcgcgcc acctcgccca cgtagatttc cacccgccgg ttttgcgcgc
     4201 gtccgtccgc tgtggcgttc gacgcgatgg gctcatggct gccgcgcccg tcgatgctga
     4261 tgcggctgga tgccaccccg cgcgaggcaa gatagtttcg cgtgctcgcc gcgcggtcac
     4321 gcgacaacgg attgttgatc gcatccgtgc cggtgctgtc ggtgtggccg acgatatgga
     4381 tctgcgtcgt ggcattggtg cgcatgcctt gtgcaaagtc gtcgagcacg cgtgccagtt
     4441 gcggcttgac ggtggagcgg ttgatgtcga aaccggcgtc actggggatg tgcaccttga
     4501 tacggttgtc ggccgtgcgt tcgacttcga tgccggtacc ggccgtcgcc tgttccattt
     4561 ccgctttctg ctgctccatg cgggtggacc agatatagcc gccgatggcg ccgacgcccg
     4621 cgccgatggc cgcgccggtg gcggtgctgc ggccgcggtt gccgcccgcc gtcgccgccc
     4681 cgatgactgc gcccgccgcg gcgccgatac cggcgccggt cgcggtatgc cgttgggtgt
     4741 cgctcattcc ggcgcagccg ctcacgacca gagcgatgag cgcgacgccg atggtttgct
     4801 gtcggtacat ggttgttccc ttttcctgga tgagagaggg tgttgttggt gccggcagta
     4861 tgccattgga atggtgccga tgcgcacgtg ctcagcgcgg tttggcggcg agggcggcga
     4921 tgacgcgtcg cagttcggag tcggccggca gttcctgctc caagcggcgt agtccttccg
     4981 ccgcacccgc cggcagcgga cgcacggtgc gcggccgcgc cggcacgggg cagtccggct
     5041 gcacttcgac tttgatgctg gcgacgtgat agccctcgcg cgcgagggct tcgaccagcg
     5101 aggctgtgca gtgcttcaat ttcgccgcga ccgcgttgcc gcccgcatgc aggatcagcg
     5161 tgtcgccctt gagattggcg acttgtgcgg cggtgcgcag attggccggc agcgagcgtt
     5221 cgaccgcgcg gcgcaaggcg agcagccttt cggcctgcgg gatcagttcg ttgccggttt
     5281 gcaggaagga gcggagcggt ttgcctgaca cgggaacagc ggagcatggt ttacacgccg
     5341 cattgtccgg tgcgccgtgt tccggggcaa tgcccaagtt tgtggactcc tgccgttgcc
     5401 gctttgaggg gcaataagct gccgcaagcc gcgtgataga ctaccgcctt cccgcttttc
     5461 ccttctctct atctccatga tttcccgggt cctaaagacc gttttcggca gtcgta
//
LOCUS       DATGOU010000084        16059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_4660247, whole genome shotgun sequence.
ACCESSION   DATGOU010000084 DATGOU010000000
VERSION     DATGOU010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16059
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4660247"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VKZ48_05165"
     CDS             <1..398
                     /locus_tag="VKZ48_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013093866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HLU77277.1"
                     /translation="KLYRAFLETAALTKWLPPYGFTCTAHEMDARVGGRFRMSFHNFS
                     TGHSHSFGGEYRKLVPGEYIEYTDRFDDPNLPGEMTVSVTLRAVSCGTEMHIEQTGIP
                     EAIPVEQCYLGWQESLIQLAHLVEPDIRD"
     gene            428..1138
                     /locus_tag="VKZ48_05170"
     CDS             428..1138
                     /locus_tag="VKZ48_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HLU77278.1"
                     /translation="MIELYSAATPNGHKISIALEELALPYTLRVIDLSANEQKQPWFL
                     KINPNGRIPAIIDRDEDDFAVFESGAILIYLAEKTGRLMPTDRKGRSRVLQWLMFQMG
                     GIGPMMGQANVFYRYFPEKIQPAIDRYQGETRRLFRVLDGQLKDHEYLAGDYSIADIA
                     NWSWVRTYKWSGVAVDDLPHLQRWLEAIAARPAVQRGIERPRSSMDDGEASEEEAQRL
                     AEEVRKMVETGQSRDSGV"
     gene            1207..1827
                     /locus_tag="VKZ48_05175"
     CDS             1207..1827
                     /locus_tag="VKZ48_05175"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLU77279.1"
                     /translation="MQSQEHWETVYATKDSHSLSWYQAHAAQSLRLIRETGVPLTAKI
                     IDVGGGASTLVDDLLADGYTGVSVLDLSATALAVAQRRLGAQAALAHWLHGDVTQIPL
                     AAHTYDIWHDRAVFHFLTDAETRRAYVDAVLRSVKPGGHVIVATFAEDGPLQCSGLPV
                     VRYDPNALHAQFGAPFALVRHLREEHQTPNGAVQQFIYCYCRKLGS"
     gene            1841..2035
                     /locus_tag="VKZ48_05180"
     CDS             1841..2035
                     /locus_tag="VKZ48_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77280.1"
                     /translation="MRRLRPGQAITPDEFEALSDAQLERLVPVRYRDYYPGKEACSDG
                     FFYLHDGSAYSFYKGGLLDD"
     gene            2064..2477
                     /locus_tag="VKZ48_05185"
     CDS             2064..2477
                     /locus_tag="VKZ48_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HLU77281.1"
                     /translation="MPGKTPKLLSGGNPQIPKGEGDAPVQAYIAAMPGWKSDIGRRMD
                     KLIARIVPGVRKAVKWNSPFYGAREQEGWFLSFHCFTHYIKVTFFRGTSLRPVPAGES
                     KHEEVRYLDIREGAFDEAQFADWVKQASKLPGERM"
     gene            2537..3088
                     /locus_tag="VKZ48_05190"
     CDS             2537..3088
                     /locus_tag="VKZ48_05190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HLU77282.1"
                     /translation="MTSEREKMLAGELYDPFDPELAAARERARDLCQDLNATREAQSE
                     ERRRILQQLFGSGGDTVWMQPPFYCDYGSNIHLGERVYFNFNCVVLDVCEVHIGDYTL
                     FGPAVQILTPLHPFEAAARREREYGKPISIGADVWVGGGALILAGVRIGNGTVIGAGS
                     VVTRDIPEGVFAAGNPCRVIRGL"
     gene            complement(3093..4568)
                     /locus_tag="VKZ48_05195"
     CDS             complement(3093..4568)
                     /locus_tag="VKZ48_05195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLU77283.1"
                     /translation="MSSAPPPATPPAPLSAAEIDQGKRILVKDAAWASLSGALQGGVI
                     MVGFALAIGAEPFVLGLLGSIPFIGQLSQLIGVAIVERVRVRKRIAVSALSVARGAML
                     ILALIPLLPESARVPVLITMQLLIAVPSSIAGCAMNSWTHQLVPREGMGAFFAHKLFW
                     ATMIGCLGSLAAGFYVDYAPFGPGPGVYSLLFAIAAAAGFVSIRYITRALEPRMYAPT
                     THGFLSRMKPPFKDRGYRKLLILMASWNFATNLAMPFLAVYLMQRLGYTLGAVTTMWV
                     TSQLANALTVYLWGRLSDRFTNKAVLSGALPLYFLCVLGLVFASAPAFDGIRLPALYA
                     VHLIMGIASGGVSLATANLGLKLAPHEQGTAYLAAVSLVGAMAGGVAPTVGGALAQWA
                     SQTELSVLVHWVTAQGSDEFTVLAFGQWEMLFAMAALLGLYVMHAMSRIEEAGPVSGR
                     AIIQEFALEALRTVNHLSTVGGASIITTLGRLVDRRRGLRK"
     gene            complement(4618..4875)
                     /locus_tag="VKZ48_05200"
     CDS             complement(4618..4875)
                     /locus_tag="VKZ48_05200"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HLU77284.1"
                     /translation="MAKYGKKASEKVEEAMREMKAGKLKSGSGKKVTNPKQAIAIGLS
                     EAREEGGKVPPPKKKAAKKKAAKKTAKKTAAKKSARKKASK"
     gene            complement(4986..7265)
                     /locus_tag="VKZ48_05205"
     CDS             complement(4986..7265)
                     /locus_tag="VKZ48_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HLU77285.1"
                     /translation="MDESLRQAALQYHRLQPPGKISVTPTKGLVNQRDLALAYSPGVA
                     AACEEIQRDPAEASMLTGRANLVAVVTNGTAVLGLGQIGPLASKPVMEGKAVLFKKFA
                     GINVFDLEIAERDPDKLVDIVAALEPTFGGINLEDIKAPECFEIERKLRERMAIPVFH
                     DDQHGTAIIVAAAIVNGLRISGKRIEDVKLVTSGAGAAALATLNLLVHLGMKREHIWV
                     TDIEGVVYEGRAALMDEYKARYARKTEARRLEEVLDGADIFLGLSAGGVLKPEMLAKM
                     APNPLILALANPEPEILPELAKQARPDSIIATGRSDYPNQVNNVLCFPFIFRGALDVG
                     ATTINEAMKAACVNAIADLAQAEQSDIVVQAYGDDSLRFGPDYLIPKPFDPRLIVKIA
                     PAVAQAAMDSGVATRPIADMDAYRNSLQQFVYHSGLIMKPLFAQAKQHRKRIVFAEGE
                     DERVLRAAQIVVDEGLAKPILIGRPSVVEGRIERYGLRLKSGVDFELVNPEWDQRYRA
                     YWQEYHRLTARRGVTVAYAKTEMRRRHTLIGAMLMRQGDADGMVCGTEGTYDEHLRYV
                     DEVIGRKPDISAYYALNVLMLQQRTVFICDTYVNRDPSAEQLCEMTLLAAQVVRRFGI
                     EPKIALLSHSSFGTSNAPSAVKMREALALITERAPELEVEGEMHGDAALSEAVREDAF
                     PNGRLKGSANLLIMPTLDAANISFNLLKASAGENVTIGPLLLGSGAPVNIVTHSATVR
                     RIVNVTVLTALEAAGIGVK"
     gene            complement(7588..8559)
                     /locus_tag="VKZ48_05210"
     CDS             complement(7588..8559)
                     /locus_tag="VKZ48_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HLU77286.1"
                     /translation="MKSLYRILVLCIAQALAGAAFAQPSYPAKPITLIVPFAAGGPTD
                     TVGRTVATAMQKRLRQSIVVENVSGAGGTVGAARVASAEPDGYTILLYHVGMSTAPAL
                     YPDLPFHPLKSFEPLGEVTDVPMTVIGRPGLEAQNLSELVTYIKRKGLIYAHAGTGSA
                     SHLCGMLLMNALGVEMKTVGHKGTGPAMHELLGGRVDLMCDQTTNTTSQIRGGKVVAY
                     GITTPLRIPSLPDVPSLHEVGLPGFEVAVWHALYAPAGTPREALDKLNDALRYALTDS
                     AVKARLSDLGTYPVSLQKATPEFAREHLIAEMEKWRPLIERARKRVD"
     gene            8832..11585
                     /locus_tag="VKZ48_05215"
     CDS             8832..11585
                     /locus_tag="VKZ48_05215"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HLU77287.1"
                     /translation="MAAAVLAAIGIALVTSLQSNLSERTVLASSVSQHEDYTTRVAAD
                     IDRQVRLARAALSELTGNITSAHLDDPAALHYFLTSRIGIQQNFESIAVFNVNGELIA
                     SRPSMEFPPVANESWFTAALRNNGTPTIREPFFSPLSGEPVVAITYPLHDEAGVLRGV
                     LMGAITLSQDQLLLSPSSAGQSKAASFVLLTHEGVIIMHPDSNYITRPLDTLGETGAL
                     IAQGMAAPGETLIGPNHEGVRSLYAFRQVPSADWTLVGVAANEAAYASLARLSRHMLL
                     AGALLAALLFPAMWLLVSRMLRPLDYLRNEMRKLRTDPAAEPTDFSASASEELRHLAE
                     DFALMAAAWRAAEGALQQEKERAEVTLESIADGVIATDAQGRIAAINRVAAKIVGWRA
                     EQAIGRDFDQVFCIEDETTGKPLDNVAVQAMKEDAVITSNRTVLRNREGELIPIDNSA
                     APIHTAHGAIDGAVVVFRNVAAERAAAQQLEWRATHDLMTGLANRAAYENAIERLYAS
                     REQDGPHALVMLDLDEFKAVNDSCGHAAGDELLKQLAKLLQTVTRRTDMVARLGGDEF
                     AVLMQSCSADKAMRVAEDLRRTVADFQFRWHERVFHVAASIGVVEIDGSFASVEEVQK
                     AADMACYVAKRNGRNRIVRHSAEDGSVDALRAERSAISSLRLAMEEGRLQLYAQPIVA
                     LDECPAMLHFEVLLRLIDTDGRVVTPAAFMPAAERYGLSDELDRWVIAQTVAACVERF
                     GPDRWDELHTVAVNLSAATLRDPRIAGYIAETVERHGMPYAKICFEVTETAAMENPEQ
                     VGALMRTLRAKGLRFALDDFGVGMTSLAQLRELPIDVLKIDGAFTRDVHTDSINGAVV
                     EAIQMMAQRLRMNTIAERVEHGAELSYLRALGVDYAQGYLLATPQPIDVVLHMTSLAA
                     ASA"
     gene            11640..12608
                     /gene="galE"
                     /locus_tag="VKZ48_05220"
     CDS             11640..12608
                     /gene="galE"
                     /locus_tag="VKZ48_05220"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HLU77288.1"
                     /translation="MKVLVVGGAGYIGSHMVLMLAERGHEVVVLDDLSSGHRDAVLAG
                     SFIEGSIADAALLDRVLAAQRFDGVMHFASFIQVGESVLEPAKYYRNNFSHTLTLLDA
                     MVRHDVKRFIFSSTAAVYGEPQRTPIDESHPCAAINPYGRSKRMVEEALADYDRAYGL
                     KSVCLRYFNAAGADPQGRIGERHEPETHLIPLVLQAAAGKRPHIAVYGNDYPTPDGTC
                     IRDYIHVVDLCAAHLLALDRLMQGSDSATYNLGNGAGFSIREVIAAAARVTGRDIAVA
                     EAARREGDPAVLVAAPDKARKELDWRPRHAALDEIIGDAWRFHSAH"
     gene            12796..13767
                     /locus_tag="VKZ48_05225"
     CDS             12796..13767
                     /locus_tag="VKZ48_05225"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HLU77289.1"
                     /translation="MSRHARVCACAAALALALCSAGLAHAAPVVTKISDPGGPTPTDM
                     VNALLSENAGITIVPGSVSYTGADNASGTFSGGGTNQANSIGIQTGIILTSGDAAFVS
                     DVPNNNGSLTSENNSPGSPLLNGLVPQGNTNNASILSFSFIPDSNQVQFSYVFGSEEY
                     NNFVNSSFNDVFGFFVNGTNFALIPGTTTPVSINNVNCGGPTGGAANGVNPSNCEFFR
                     DNPPGSGTIDTQLDGLTVVLSFVADVNPGVENTIELGIADVSDSALDSAVFIAAGTFQ
                     SCGGPDQPPCGGPGPGPGPDPGQVPEPGSLALLGLGLLGLLAGRRFR"
     gene            13937..>16059
                     /gene="pepN"
                     /locus_tag="VKZ48_05230"
     CDS             13937..>16059
                     /gene="pepN"
                     /locus_tag="VKZ48_05230"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HLU77290.1"
                     /translation="MREPTPQTIYLKDYTPPAFLIDRVDLDIDIRDDHARVTATLAMR
                     RNDRTKNGDAPLRLDGDELTLEAVSLDGRALSESEYGVDAKQLTIPGVPQAFRLQTVT
                     RIVPQENTQFMGLYASNDGYFTQCEAEGFRRITWFLDRPDVMARYTTTIHADKARYPV
                     LLSNGNLVAQGDEADGRHWARWDDPFPKPSYLYAMVAARLDKLEDTFVTRSGKQAKLA
                     IYVEPGKLDQCGFAMDCLKRCMKWDEEVFGLELDLDGYMIVAVGDFNMGAMENKGLNI
                     FNTKYVLARPDIATDMDFMNIDRVVAHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQ
                     EYGADTYSRPVTRIQEVRGLRAAQFPEDAGPMAHPVRPQSYMEISNFYTPTVYEKGAE
                     VVRMIHTLIGREAFRRGMDLYFERHDGQAVTTDDFVAAMADASGVDLTQFKRWYDQAG
                     TPVLQTSSAYDAAAKTYTLTVRQSCPPTPGQTEKLPFHIPFALGLVGPDGNDLPLQLQ
                     GERAPGGTHRVLSVKEAEEQFVFVNVPSEPVPSLLRNFSAPVILRHDYDDAALTHLVA
                     HDSDAFNRWEAGQRLATRLLLRNVEAIRAGDAPATPDSFIAAFTRVLADAHHDPAFAA
                     EALALPSEAYVGEQMEVIDPEAVHAARIALRRDIAARLQAELLETYHAHTPQGAYSPD
                     AVSAGRRALRNLCLGYLMELEDEAVL"
BASE COUNT         2882 a         5329 c         5175 g         2673 t
ORIGIN      
        1 acaaacttta tcgcgccttc ctcgaaaccg ccgcgctgac caagtggctg ccgccatacg
       61 gcttcacctg cacggcgcat gagatggatg cgcgcgtcgg cggccgcttc aggatgtcgt
      121 tccataattt ctccaccgga cacagccact ccttcggcgg cgagtaccgc aagctggtcc
      181 ccggggagta cattgaatat acggatcgct tcgacgaccc gaacctcccc ggcgagatga
      241 cggtgagcgt cacgctcaga gccgtctcct gcggcacgga aatgcacatc gagcagaccg
      301 gcattccgga ggcgattccc gtggagcaat gctatctggg ctggcaggaa tccctgatcc
      361 agcttgcgca tctggtggag ccggacatcc gcgattgatc cggcgcccga ttcaaccgag
      421 gactgtcatg atcgagcttt actctgcggc cacacccaac ggccacaaga tttccattgc
      481 gctggaggaa ttggcgctgc cctacacgct gcgcgtgatc gatctttccg cgaacgagca
      541 gaagcagccg tggtttctga agatcaatcc gaacggacgc attccggcga tcatcgatcg
      601 cgacgaagac gatttcgcgg tgttcgagtc cggcgccatc ctgatctatc tcgcggagaa
      661 gaccggccgg ctgatgccca ccgaccgcaa gggccgctcg cgcgtactgc agtggctgat
      721 gttccagatg ggcggcatcg gcccgatgat gggccaggcg aacgtgttct accgctactt
      781 cccggagaag atccagcccg ccatcgatcg ctatcagggc gagacgcgtc gcctgttccg
      841 cgtgctggac ggccaactga aggatcacga gtatctggcc ggcgactact ccatcgcgga
      901 catcgccaac tggtcctggg tgcgcaccta caagtggtcc ggcgtggcgg tcgatgatct
      961 gccgcatctg cagcgttggc tcgaggcgat cgccgcacgc cccgcggtgc agcgcggcat
     1021 cgaacgcccg cgctcctcga tggacgacgg cgaggcgagc gaggaggaag cgcagcggct
     1081 cgccgaggaa gtgcgcaaaa tggtcgagac cggccagtcg cgcgatagcg gtgtgtaagc
     1141 gagcgcacgt gttgccagac cgacgttctg aagcgtagat tgccggatac gccgaaggaa
     1201 aaacgcatgc aatcgcagga acactgggaa accgtttacg cgaccaagga ttcgcacagc
     1261 ctgagctggt atcaggcgca cgccgcgcaa tcgctgcgac tgatccgcga gaccggcgtg
     1321 cccttgacgg cgaaaatcat cgacgtcggc ggcggcgcct cgacgctcgt cgacgatctg
     1381 ctggcggacg gctataccgg cgtgtcggta ctcgacctgt cggccaccgc gctcgccgtg
     1441 gcgcagcgcc gtctgggcgc acaggccgcg ctagcgcact ggctgcatgg cgacgtcacg
     1501 cagattccgc tggcggcgca cacctacgac atctggcatg accgcgccgt tttccacttc
     1561 ctgaccgacg cggaaacgcg tcgggcgtac gtcgatgccg tacttcgctc ggtcaagccg
     1621 ggcggtcatg tcatcgtggc gacgttcgcc gaggacggcc cgctgcaatg cagcggtctg
     1681 ccggtcgtac gctacgaccc gaatgcgctg catgcgcaat tcggcgcgcc cttcgcgttg
     1741 gtgcggcatc tgcgcgaaga acatcagaca ccaaacgggg ccgtgcagca gttcatttac
     1801 tgctactgcc ggaagctggg ctcctgaccc ctcgacaacc atgagaagac taagacccgg
     1861 gcaggcgatc accccggacg aattcgaagc cctctccgac gcgcaactcg aacgcctcgt
     1921 gccggtgcgc tatcgggatt actaccccgg caaggaagcc tgcagcgacg gcttcttcta
     1981 tttgcacgac ggcagcgcat acagcttcta caaaggcggc ctgctcgacg actgagccgc
     2041 cgctccgaca tggagtgaga accatgcccg gcaagacgcc caagctgctc tccggcggca
     2101 atccgcagat tccgaaaggc gaaggcgacg cgccggtgca ggcctacatc gccgccatgc
     2161 ccggctggaa aagcgacatc ggtcgccgca tggacaagct catcgcgcgc atcgtccctg
     2221 gggtgcgcaa ggcggtgaaa tggaactcgc ctttctacgg cgccagggag caggaaggct
     2281 ggttcctttc cttccactgc ttcacgcatt acatcaaggt gacgttcttc cgcggtacct
     2341 cgctgcgccc cgttccggcc ggcgagtcca agcacgagga agtgcgatat ctggacatcc
     2401 gcgaaggcgc gttcgacgaa gcgcagtttg ccgactgggt gaagcaggcg agcaaactgc
     2461 cgggcgaacg catgtagcgc gacggccgat gtaccgcaac ctgtgtcgcg atgtgcggcc
     2521 ttgaagcgag gcaaccatga cgagcgaacg cgagaagatg ctggccgggg aactctacga
     2581 ccccttcgat cccgaactcg cggcggcgcg ggaacgcgca cgcgatttgt gccaggacct
     2641 caatgccaca cgcgaagcgc aaagcgagga gcgacgacgc atcctgcagc agcttttcgg
     2701 cagcggcggc gacaccgtat ggatgcagcc gccgttctac tgcgactacg gctcgaacat
     2761 tcatctcggc gagcgcgtgt acttcaactt caactgcgtg gtgctggacg tgtgcgaggt
     2821 gcacatcggc gactacacgc tgttcggtcc ggcggtgcag atccttacgc ccctgcatcc
     2881 cttcgaagcc gccgcccgtc gcgagcgcga atacggcaag ccgatttcca tcggtgcgga
     2941 cgtatgggtg ggcggcggcg cgctgatcct cgccggcgtg cgcatcggca acggcacggt
     3001 catcggtgcg ggcagcgtgg tgacgcgcga cattcccgaa ggcgtgttcg cggcgggcaa
     3061 cccctgccgc gttatccgcg ggctttagcg cctcacttgc gcaaaccgcg ccgccggtcc
     3121 accagacggc cgagggtagt gatgatgctc gcgccgccga cggtggacag gtggttcacc
     3181 gtgcgcagcg cctccagcgc gaactcctgg atgatggcgc gaccgctgac cggaccggcc
     3241 tcttcgatgc gcgacatcgc gtgcatgacg tacagcccga gcagcgcggc cattgcgaac
     3301 agcatctccc attggccgaa ggcgagtacg gtgaattcgt cgctgccctg cgccgtcacc
     3361 cagtgcacca ggaccgacag ctcggtctgg ctggcccatt gcgccagcgc accgccgacg
     3421 gtcggcgcca cgccgcccgc catcgcaccg accagactca cggccgcaag ataggcggtg
     3481 ccctgttcat gcggcgcgag cttcaagccc aggttggcgg tggcgaggct gacgccgccc
     3541 gaagcgatgc ccatgatgag gtgcaccgca tagagcgcgg gcaggcgtat gccatcgaag
     3601 gccggtgccg aagcgaacac caggccgagc acgcagagga aatagagcgg cagcgcgccg
     3661 ctcagcaccg ccttgttggt aaagcggtcc gacagccggc cccacagata gaccgtgagc
     3721 gcattggcga gctggctcgt cacccacatc gtggtcacgg cgccgagcgt gtaaccgagc
     3781 cgctgcatca gatagacggc gagaaaaggc atcgccagat tggtcgcgaa attccacgac
     3841 gccatgagaa tgagcagctt gcgatagccg cgatccttga acggcggctt catgcgcgaa
     3901 aggaagccat gcgtggtcgg cgcgtacatc cgcggttcga gcgcgcgagt gatgtagcgg
     3961 atgctgacga aaccggcggc ggccgcaatg gcgaacagca gcgaatacac gcccggtccg
     4021 ggcccaaagg gcgcgtagtc cacgtagaag ccggcggcca gcgaaccgag gcagccgatc
     4081 atcgtcgccc agaacagctt gtgcgcgaag aaggcgccca tgccttcgcg cggcacgagc
     4141 tgatgcgtcc acgaattcat ggcgcagccg gcgatcgaac tgggcacggc gatcaaaagc
     4201 tgcatggtga tgagcaccgg cacccgcgcg gattcgggca gcagtggaat cagcgcgagg
     4261 atgagcatcg cgccgcgggc gacggagagc gcactcaccg cgatccgctt gcgcacgcgc
     4321 acgcgctcga cgatggccac gccgatcaac tgcgacaact gtccgatgaa gggaatggag
     4381 cccagcaggc cgaggacgaa aggttccgcg ccgatcgcca gcgcaaagcc caccatgatc
     4441 acgccgccct gcagcgcgcc ggacagactg gcccaggcgg catcctttac aagaatgcgc
     4501 ttgccttggt cgatttccgc ggcggataag ggcgcgggcg gcgtcgcggg gggaggcgct
     4561 gaggacacga tcggggcgca tcaataagcg tgccgatcgc cggtgcgcgc gctgtgacta
     4621 cttcgacgcc ttcttccggg cggatttttt cgccgctgtc tttttcgccg tctttttggc
     4681 ggccttcttt ttcgccgcct tcttcttcgg cggcggcacc ttgccgccct cttcgcgcgc
     4741 ctcggacagg ccgatggcga tggcctgctt ggggttggtg accttcttgc ccgagccgct
     4801 cttgagcttg cccgccttca tttcgcgcat cgcctcctcg actttctcgg aggccttctt
     4861 gccgtatttc gccatgtcgc gcgctctcag gttccgacat ggcgtcgcct gcaagatacc
     4921 gaccggcccg gcggccgaaa ccaaaagggc ggccgatggc cgcccttcgg ttacagcctt
     4981 gccgcctact tcacgccaat gccggccgcc tccagcgcgg taagcaccgt gacgttgacg
     5041 atgcgtcgca ccgtggccga gtgcgtgacg atattgaccg gcgcgcctga gccgagcagc
     5101 agcggaccga tggtgacgtt ctcgcccgcc gaagccttga gcaggttgaa ggagatattg
     5161 gcggcgtcca gcgtcggcat gatgagcaga ttggccgagc ccttgaggcg cccgttgggg
     5221 aaggcgtcct cgcgcaccgc ttccgacagc gcggcgtcgc cgtgcatctc gccttccact
     5281 tccagttccg gcgcacgttc ggtgatcagt gccagcgcct cgcgcatctt caccgcactg
     5341 ggcgcgttgc tggtgccgaa gctggaatgc gagagcagcg cgatcttggg ttcgatgccg
     5401 aagcggcgca ccacctgcgc ggccagcagc gtcatctcgc acaactgttc ggcgctcggg
     5461 tcgcgattga cgtaggtgtc gcagatgaac accgtgcgct gctgcagcat cagcacgttg
     5521 agcgcgtaat aggcgctgat gtccggcttg cggccgatca cttcgtccac gtagcgcaga
     5581 tgttcgtcat aggtgccttc ggtgccgcac accatgccgt cggcgtcgcc ctggcgcatg
     5641 agcatcgcgc cgataagcgt atggcgccgg cgcatttcgg tcttggcgta ggcgacggtc
     5701 acgccgcggc gcgcggtcag gcggtggtac tcctgccagt aagcgcggta gcgctgatcc
     5761 cactccggat tcaccaattc gaaatcgacg ccggatttga gccgcaggcc gtagcgttcg
     5821 atgcgcccct ccaccaccga cggccgcccg atgagaatgg gtttggccag cccttcgtcc
     5881 accacgatct gcgcggcgcg cagcacgcgc tcgtcctcgc cctcggcgaa cacgatgcgc
     5941 ttgcgatgct gcttcgcctg cgcgaacagc ggcttcatga tcaggccgga gtggtacacg
     6001 aactgctgca gcgagttgcg gtacgcgtcc atgtcggcga tgggccgcgt ggcgacgccg
     6061 ctgtccatcg ccgcctgcgc cacggccggc gcgatcttga cgatcagacg cggatcgaag
     6121 ggcttgggaa tgaggtagtc ggggccgaag cgcagactgt cgtcgccgta ggcctgcacc
     6181 acgatgtccg actgctccgc ctgcgcgaga tcggcgatcg cattcacgca ggcggccttc
     6241 atcgcttcgt tgatggtggt cgcgcccaca tcgagcgcgc cgcggaagat gaacggaaag
     6301 cacaggacgt tgttgacctg gttcgggtag tccgaacggc cggtggcgat gatggagtcc
     6361 gggcgcgcct gcttggccag ttcgggcagg atttccggct ccggattggc gagcgcgagg
     6421 atcagcgggt tcggcgccat cttcgccagc atctccggct tcagcacacc gccggcggac
     6481 aggccgagga agatgtcggc gccgtccagc acttcctcga ggcgacgcgc ctcggtcttg
     6541 cgcgcataac gcgccttgta ttcgtccatc agcgcggcgc ggccctcgta caccacgcct
     6601 tcgatgtccg tcacccagat gtgttcgcgc ttcatgccca ggtgcacgag caggttgagc
     6661 gtggcgagcg cagcggcgcc cgcgccggag gtgacgagct tgacgtcctc gatgcgcttg
     6721 ccgctgatgc gcagcccgtt gacgatggcc gctgcgacga tgatcgcggt gccgtgctgg
     6781 tcgtcgtgga aaaccggaat cgccatgcgc tcgcgcagct tgcgttcgat ttcgaagcac
     6841 tcgggcgcct tgatgtcttc caggttgata ccgccgaaag tcggctccag cgcggcgacg
     6901 atgtccacca gcttgtccgg gtcgcgctcg gcgatttcca gatcgaagac gttgatgccg
     6961 gcgaatttct tgaacagcac ggccttgcct tccatcaccg gcttggacgc gagcgggccg
     7021 atctgcccca accccagcac ggcggtgccg ttggtgacga cggcgacgag gttcgcgcgg
     7081 ccggtgagca tgctcgcttc ggccggatcg cgctggatct cctcgcaggc cgcagccacg
     7141 ccgggcgaat aggccagcgc cagatcgcgc tggttcacca gccccttggt cggcgtgacg
     7201 gagatttttc cgggcggctg caaacggtgg tactggagtg cggcctgacg tagcgattcg
     7261 tccatatcct ttcttcctca agtcacggcg atgctgcaca agcaggattg cagcacaccc
     7321 cggcgtaaaa cgctcgtgtg cgccggacgc cggccgtcgc ggcgtccttg ccgggaagca
     7381 ataaacgggg cgcgggtcga ttccgggttg aagattcgtc ggccgcaagc ggggcgcacg
     7441 cgcccgatgc ggcgcggtgg cggtgtgaaa gacctcgccg ggaggcaagg ccgggccgac
     7501 tgcatgacat cgacatgcag tatagcgaat ccgccgcagg cagcggaacg gaacgcaagc
     7561 ggcacgcggg gcgcgtgccg cggggggtca atcgacgcgc ttccgcgccc gctcgatcag
     7621 cggccgccac ttctccattt ccgcgatcag gtgttcgcgg gcgaattcgg gcgtggcttt
     7681 ttgaagcgag accggatagg tgcccaggtc gctcaggcgc gccttcaccg ccgaatcggt
     7741 gagcgcatag cgcagcgcgt cgttgagctt gtcgagcgcc tccctgggcg tgcccgccgg
     7801 ggcgtagagc gcgtgccaga cggccacctc gaagcccggc agcccgactt cgtgcaggct
     7861 cggcacgtcg ggcagggagg gaatgcgtaa cggcgtggtg atgccgtagg cgacgacctt
     7921 gccgccgcga atctgcgagg tggtgttggt ggtctggtcg cacatcaggt ccacgcgacc
     7981 accgagcagt tcgtgcatcg ccgggccggt tcccttgtgg ccgaccgtct tcatttccac
     8041 gcccagcgca ttcatcagca gcatgccgca cagatgcgag gcggaacccg tgccggcgtg
     8101 cgcgtaaatc aaccccttgc gcttgatata cgtcaccagt tccgaaaggt tctgcgcttc
     8161 cagtccgggc cggccgatca ccgtcatcgg cacatcggtc acttcgccca gcggctcgaa
     8221 gctcttcagc ggatggaaag gcaggtcggg atagagcgcg ggcgcggtgg acatgcccac
     8281 gtggtagagc aggatggtgt agccgtccgg ctcggcactc gcaacacggg cggcgcccac
     8341 ggtgccgccc gcgccgctga cgttttcgac gacgatgctc tggcgcaggc gtttttgcat
     8401 cgccgtcgcc acggtgcgtc cgacggtgtc ggtcgggccg ccggccgcga acggaacgat
     8461 cagcgtaatc ggtttggccg gataagacgg ctgcgcgaac gctgcgccgg ccaatgcctg
     8521 ggcaatgcac aacactagga tccggtacag cgatttcatt cgacctgcct gtcctgttgt
     8581 tgaatttgtc agattaacga ccgttatcgg tcgcgatacg tcgaaaagac ctcgttttcc
     8641 acttcaaaac cacccctcgt gtaccggggg gccgggcaat atgcgcggat gcgcttccat
     8701 gcgccgggac acctcgccgc gtcggccgta tcgaccttcc gcgagggata caggcgaaga
     8761 gcctacacgt cccgcaataa aaaaaccaaa aaggggagtc ggtgctcaga acactgaagg
     8821 gaaggactgc gttggccgca gccgtcctcg ccgcaatcgg cattgcgctg gtcaccagcc
     8881 tgcagtccaa cctgtccgaa cggaccgtcc tggcgtcgag cgtcagccag cacgaggact
     8941 acaccacccg cgtcgccgcg gacatcgacc gtcaggtgcg tcttgcgcgc gccgcgttga
     9001 gcgaactgac cggcaacatc accagcgcgc acctggacga cccggccgca ctgcattact
     9061 tcctcacctc gcgcatcggc atccagcaga acttcgagag catcgcggtg ttcaatgtga
     9121 acggcgagct gatcgcttcg cgcccgtcga tggagtttcc cccggttgcg aacgaaagct
     9181 ggttcacggc ggcgctgcgc aacaacggca cgccgaccat ccgcgaacct tttttcagcc
     9241 cgctcagcgg cgaacccgtc gtggcgatca cctatccgct gcacgacgag gccggcgtgc
     9301 tgcgcggcgt cctgatgggc gcgatcacgc tgagccagga tcagttgctg ctgtcgccct
     9361 cttccgccgg acaaagcaag gcggcgagtt tcgtgctgct cacgcacgaa ggcgtgatca
     9421 tcatgcaccc ggattccaac tacatcaccc ggccgctcga cacgttgggc gaaaccgggg
     9481 cgctgatcgc gcaaggcatg gccgcgccgg gcgagacgct gatcggcccg aatcacgagg
     9541 gggtgcgcag tctttatgcg ttccggcagg tcccttcggc cgactggacg ctggtgggcg
     9601 tggccgccaa cgaagcggcc tacgcttcgc ttgcgcgcct gtcccggcac atgctgctcg
     9661 ccggcgcgct gctcgccgcc ctgctgttcc cggcaatgtg gctgctcgtc tcgcgcatgc
     9721 tgcggccgct cgactatctg cgcaacgaaa tgcgcaagct gcgcaccgac ccggccgccg
     9781 agccgaccga cttctccgca tcggcaagcg aggagctgcg ccacctcgcc gaggacttcg
     9841 cgctgatggc cgcggcgtgg cgcgccgccg aaggtgcact gcagcaggaa aaggaacgcg
     9901 ccgaggtcac gctggaatcg atcgccgacg gcgtcatcgc caccgatgcg cagggccgca
     9961 tcgcggcgat caaccgcgtg gcggccaaga tcgtcggctg gcgcgccgag caggccatcg
    10021 gccgcgactt cgaccaggtg ttctgcatcg aggacgaaac caccggcaag ccgctggaca
    10081 acgtcgccgt gcaggcaatg aaagaggatg cggtgatcac ctccaaccgg acggtgctgc
    10141 gcaaccgcga aggcgaattg atccccatcg acaattccgc cgcgcccatc cacacggcgc
    10201 acggcgcgat cgacggcgcc gtcgtggtgt tccgcaacgt cgccgccgaa cgcgccgccg
    10261 cgcagcaact ggagtggcgc gccacccacg atctgatgac cgggctggcc aaccgtgccg
    10321 cctacgaaaa cgccatcgag cggctgtatg cgtcgcgcga gcaggacggc ccgcacgcgc
    10381 tggtgatgct cgacctggac gaattcaagg ccgtcaacga ttcctgcggc cacgccgccg
    10441 gagacgaatt gctgaaacaa ctggccaagc tgctgcaaac ggtcacgcgc agaacggaca
    10501 tggtcgcccg cctcggcggc gacgagttcg ccgtgctcat gcagtcctgc tccgcggaca
    10561 aggcaatgcg cgtcgcggag gatctgcggc gcaccgtggc cgacttccag ttccgctggc
    10621 acgagcgcgt cttccacgtc gccgcgagca tcggcgtggt cgagatcgac ggcagcttcg
    10681 cttccgtgga ggaagtgcag aaagccgccg acatggcctg ctacgtcgcc aagcgcaacg
    10741 gccgcaaccg catcgtgcga cacagtgccg aggacggcag tgtcgatgcg ctgcgcgcgg
    10801 agcgctcggc gatcagcagc ctgcgtctgg ccatggagga agggcggctg cagttgtacg
    10861 cccagccgat cgtcgcgctg gacgaatgtc ccgcgatgct gcacttcgaa gtgctgctgc
    10921 gcctgatcga caccgatggg cgcgtggtga cgccggcggc gttcatgccg gccgccgaac
    10981 gctacggctt gagcgacgaa ctcgatcgct gggtaatcgc gcagacggtc gcagcctgcg
    11041 tggaacgctt cgggccggac cgctgggacg aactgcacac ggtggccgtc aacctgtccg
    11101 cagccacgct gcgcgatccg cgtatcgccg gctacatcgc cgaaacggtg gaacgccacg
    11161 gcatgcccta cgccaagatc tgcttcgagg tgacggaaac cgctgcgatg gagaatccgg
    11221 agcaggtcgg cgcgctgatg cgcacgctgc gcgccaaggg tctgcgcttc gcgctggacg
    11281 atttcggcgt ggggatgacg tcgctggcgc aactgcgcga actgccgatc gacgtgctga
    11341 agatcgacgg cgcgttcacg cgcgacgtgc acacggattc gatcaacggc gcggtggtcg
    11401 aagcgatcca gatgatggcc cagcggttgc ggatgaacac tatcgcggaa cgcgtggagc
    11461 acggtgcgga actcagctat ctgcgcgccc tgggtgtcga ttacgcccag ggctatctgc
    11521 tcgccacacc gcagcccatc gacgtcgtgc tgcacatgac ctcgctggcc gcggcctccg
    11581 cctagggccg cctagcgccg cgcgctatca tcgcgcctcc gcttccatcg aggacgtcca
    11641 tgaaagtgct ggttgtcggc ggcgccggtt acatcggctc gcacatggtg ctgatgctcg
    11701 ccgagcgcgg ccatgaagtc gtggtgctgg acgatctgtc cagcggccat cgcgacgcgg
    11761 tgctcgccgg cagtttcatc gaaggcagca tcgccgacgc ggcattgctc gatcgcgtcc
    11821 ttgccgcgca gcgcttcgac ggcgtcatgc acttcgcctc gttcatccag gtcggcgaat
    11881 ccgtcctcga accggcgaag tactaccgca acaacttcag ccacacgctg acgctgctcg
    11941 atgcgatggt gcgtcacgac gtcaaacgtt tcatcttctc ctccactgcc gcagtgtacg
    12001 gtgaaccgca gcgcacgccg atcgacgagt cgcatccctg tgcggccatc aatccctatg
    12061 gccgctccaa gcgcatggtc gaagaggcgc tcgccgatta cgaccgcgcc tacggtctga
    12121 aaagcgtctg cctgcgttac ttcaatgcgg cgggcgccga cccgcagggc cgcatcggcg
    12181 aacggcacga acccgagacc catctcatcc cgctggtgct gcaggcggcg gccggcaagc
    12241 ggccgcacat cgccgtgtac ggcaacgact atccgacgcc ggacggcacc tgcatccgcg
    12301 actacatcca cgtcgtcgat ctttgcgcgg cgcacctgct cgcgctcgac cgcctgatgc
    12361 aaggcagcga cagtgcgacg tacaacctcg gcaacggcgc cggcttctcc atacgtgaag
    12421 tgatcgcagc ggcggcccgg gtgaccggcc gcgacattgc cgtggcggaa gccgcgcggc
    12481 gcgaaggcga tccggcggtg cttgtcgccg caccggacaa ggcgcgcaag gaattggact
    12541 ggcgaccgcg gcacgctgcg ctggacgaga tcatcggcga tgcctggcgc ttccactccg
    12601 cgcactaaaa atttgtccat atcgtcgcgc gcgctgtagt gattcagcga caagcgaaac
    12661 gtcgcaccgt cttactgtgc gacgcgtatc ctgacgagga catttcaata aaccactgag
    12721 cctctgagca aatccgtctc cggcacgcgc tctgcaaaaa aatgtgcggg cgaggatcac
    12781 aggacggagg gcatcatgag cagacatgcc agagtgtgcg cgtgcgcggc tgcgcttgcg
    12841 cttgcgctat gcagcgcggg cctcgcgcac gccgcaccgg tggtaacgaa gatctccgac
    12901 ccgggcgggc cgacgcccac cgacatggtg aacgcgctat tgagcgagaa tgccggcatc
    12961 accatcgttc cggggtcggt ctcctatacc ggcgccgaca atgcttcggg caccttctcc
    13021 ggcggcggaa cgaatcaagc gaactcgatc ggcattcaga ccggcatcat cctcacttcg
    13081 ggcgacgccg cctttgtcag cgacgtgccg aacaacaacg gcagcctgac ctcggagaac
    13141 aattcgcccg gcagcccgct gctgaacggg ctggttccgc aaggcaacac caacaatgcc
    13201 tccatcctga gcttcagctt cataccggac agcaaccagg tgcagttcag ctatgtgttc
    13261 ggatcggagg agtacaacaa cttcgtcaac agcagtttca acgacgtgtt cggcttcttc
    13321 gtcaacggga cgaacttcgc gttgattccc ggcacgacga cgcccgtgtc gatcaacaat
    13381 gtcaactgcg gcggaccgac cggcggcgca gcaaacggcg tgaatccctc gaactgcgag
    13441 ttcttccgcg acaacccgcc cggctcggga acgatcgata cgcaactcga cgggctgacg
    13501 gtggtgctgt ccttcgttgc ggatgtgaat ccgggcgtgg agaacaccat cgaactgggc
    13561 atcgcagacg tgagcgacag cgcactcgac tcggcggtat tcatcgccgc ggggacgttc
    13621 cagtcctgcg gcggtcccga tcaaccgccc tgcggcggcc ccggacccgg accgggcccc
    13681 gatccgggtc aggtgcctga acccggcagc ctcgcattgc tgggactggg actgctggga
    13741 ctgctcgcgg ggcgccggtt ccgctgagtg cgcgcatcac cggcaagggc ggccgcaggc
    13801 cgcccttttt tattgggcgc atcgaaccgc ttcaccggcc tggccggccg gtgccttgca
    13861 gggggtttat gatcggggtc cgggcacagc acgcacgtcc gcgcccgatt ctcctccact
    13921 tcgttgttcc ggaaccatgc gcgaacccac tccccaaaca atttatctca aggattacac
    13981 cccacccgct tttctgatcg atcgggtgga tctcgacatc gacattcgcg acgaccacgc
    14041 gcgggtcacg gcgacgctcg cgatgcgacg caacgaccgg acgaagaacg gcgacgcccc
    14101 cctgcgcctc gacggcgacg aactgacgct cgaagccgtg tcgctcgacg ggcgcgcgct
    14161 gagcgaaagc gaatacggcg tcgatgcgaa acaactgacg attcccgggg tgccgcaggc
    14221 gttcaggctg cagaccgtca cgcgcatcgt gccgcaggaa aacacgcagt tcatgggtct
    14281 gtacgcgtcg aacgacggct acttcaccca gtgcgaggcg gaaggcttcc gccgcatcac
    14341 ctggttcctc gaccggcccg acgtgatggc gcgctatacg acgaccatcc acgccgacaa
    14401 ggcgcgctat ccggtactgc tgtccaacgg caacctcgtc gcacagggcg acgaagccga
    14461 cggccgccat tgggcgcgct gggacgatcc ctttcccaag ccgtcgtacc tctacgccat
    14521 ggtcgcggcg aggctcgaca agctggagga caccttcgtc acgcgctcgg gcaaacaggc
    14581 gaagctcgcc atctatgtgg agccgggcaa gctcgatcaa tgcggcttcg ccatggactg
    14641 cctcaagcgc tgcatgaagt gggacgaaga agtcttcggc ctggaactcg atctcgacgg
    14701 ttacatgatc gtggccgtgg gcgacttcaa catgggcgcg atggagaaca agggcctcaa
    14761 catcttcaac accaagtacg tgttggcgcg ccccgacatc gcgaccgaca tggacttcat
    14821 gaacatcgac cgcgtggtcg cgcacgagta cttccacaac tggaccggca accgcgtcac
    14881 ctgccgcgac tggttccagt tgtcgctcaa ggaagggctc acggtgttcc gcgaccagga
    14941 atacggcgcc gacacctact cgcgtccggt gacgcgcatc caggaagtgc gcggtctgcg
    15001 cgcggcgcag tttcccgagg acgccggccc gatggcgcat ccggtgcggc cgcagtcgta
    15061 catggagatc agcaacttct acacgcccac cgtgtacgag aaaggcgcgg aagtggtgcg
    15121 catgatccat acgctgatcg gcagggaagc gttccgacgc ggcatggatc tttatttcga
    15181 gcgccacgac ggacaggccg tcaccaccga cgacttcgtc gccgccatgg cggacgcgtc
    15241 gggcgtcgat ctcacgcaat tcaagcgctg gtacgaccag gccggcacgc cggtgctcca
    15301 aacgagcagc gcatacgacg ccgcagcaaa gacctatacg ctcaccgtgc gccaaagctg
    15361 cccgcccacg cccggtcaga cggagaagct gccgttccac attcccttcg cgctcggcct
    15421 ggtgggcccc gacggcaacg atctgccgct gcaactgcaa ggcgagcgcg cgccgggcgg
    15481 cacgcaccgc gtgctgtcgg tgaaggaggc ggaggagcag ttcgtgttcg tcaatgtgcc
    15541 gagcgagccc gtgccctcgc tgctgcgcaa cttctccgcg ccggtgatcc tgcggcacga
    15601 ttacgacgac gcggcgctga cgcatctggt ggcgcacgac tcggacgcct tcaatcgctg
    15661 ggaagccggc cagcggctcg ccacgcgact gctgctgcgc aatgtcgagg cgatccgcgc
    15721 cggcgacgcg ccggcgacgc ccgattcgtt catcgccgcc ttcacgcgcg tactggccga
    15781 tgcgcatcac gatcccgctt tcgccgccga agcgctggcg ctgccgtccg aagcctatgt
    15841 cggcgaacag atggaggtga tcgatccgga ggccgttcat gccgcgcgca tcgcattgcg
    15901 cagagacatc gccgcgcgac tgcaggccga actgctcgag acctatcacg cacacacgcc
    15961 gcaaggcgcc tacagtccgg acgcggtttc ggccggccgc cgcgcgctgc gcaatctctg
    16021 cctgggctat ctgatggaac tggaagacga ggcggtgct
//
LOCUS       DATGOU010000085         4780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_1558896, whole genome shotgun sequence.
ACCESSION   DATGOU010000085 DATGOU010000000
VERSION     DATGOU010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1558896"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..909)
                     /gene="dapD"
                     /locus_tag="VKZ48_05235"
     CDS             complement(88..909)
                     /gene="dapD"
                     /locus_tag="VKZ48_05235"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HLU77291.1"
                     /translation="MMQAHQSLIDQAWEDRANVSPASTDQKLRDAVEEVLAGLDRGAL
                     RVAEKIDGRWQTHQWLKKAVLLSFRLNDNEVMPGASTRYYDKVPGKFANYGAADFAAG
                     GFRVVPNAMVRRGAHIGRNVVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKGVHLS
                     GGVGIGGVLEPLQANPTIIEDNCFIGARSEIVEGVIVEENSVISMGVFIGMSTRIYNR
                     LTGETLYGRVPAGSVVVPGNLPSADGKYSLYAAVIVKQVDAKTRAKTSINELLRE"
     gene            complement(1068..2258)
                     /gene="dapC"
                     /locus_tag="VKZ48_05240"
     CDS             complement(1068..2258)
                     /gene="dapC"
                     /locus_tag="VKZ48_05240"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HLU77292.1"
                     /translation="MNPDLGKLQPYPFERLRKLYAAVTPDSKYTPISLSIGEPKHATP
                     AFIQRALADNLTGLAAYPATAGSDSLRQAIADWLQIRYDLPKIDADTQVLPVNGSREA
                     LFAIAQVVVDRTRDAVVVCPNPFYQIYEGAALLAGATPYYVNAHRDNDFAIDYGQVPD
                     SVWQRTQLLYCCSPSNPTGRVMSLDEWRTLFELSDRHDFVIVSDECYSELYLDEKQPP
                     LGALEAANKLGRTGFPRLIVFSSLSKRSNVPGMRSGFVAGDAQLMRQFLLYRTYHGSA
                     MSPSIQEASEAAWRDEAHVAENRRLYREKFLAARELLAEPLQTELPVGGFYLWIRTPI
                     DDAEFARGLYANYNVAVLPGSYLAREAHGVNPGAQYIRVALVAPLKECLDGLKRLNAH
                     AQSL"
     gene            complement(2268..2684)
                     /gene="queF"
                     /locus_tag="VKZ48_05245"
     CDS             complement(2268..2684)
                     /gene="queF"
                     /locus_tag="VKZ48_05245"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HLU77293.1"
                     /translation="MATQPSTELQHFPNPSPERDFLIHMEIPEFTCLCPMTGQPDFAV
                     LYLDYVADRKCVELKSLKLYIWSYRNEGAYHEAVTNKILNDLVQLLEPRFMRLTAKFY
                     VRGGIFTTVVAEHRKEGWTPQIAIPQLSFEAQSNIR"
     gene            2906..>4780
                     /gene="smc"
                     /locus_tag="VKZ48_05250"
     CDS             2906..>4780
                     /gene="smc"
                     /locus_tag="VKZ48_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HLU77294.1"
                     /translation="MRLTQLKLAGFKSFVDPTTIHVPGQHVGVVGPNGCGKSNIIDAV
                     RWVLGESRASALRGESMQDVIFNGSTLRKPVARASVELVFDNSLGKAAGQWSQYAEIS
                     VKRILTREGDSSYWINNTQVRRRDVQDIFLGTGVGPRAYAIIEQGMISRVIEAKPEEL
                     RVFLEEAAGVSKYKERRKETENRLEDTRENLARVEDIRGELDAQIEKLTQQAEIARQY
                     KTYQTEARDKQNLLWYLRKQDAEADAQRLQRDIEQAVNALEAETARLREIEARLEAVR
                     AEHYAAGDAVNAAQGEFYGVNTEVSRLETEIRYVGEKRQRLEAQAAQLDMQVEQWQRQ
                     REDLADALTMWRDRREAAAERLALAEARAEEEQARLPDAEEAFRALQERLGEARDAIA
                     RAEQAFQVEQTHLTHTGRTLQQLDVREERLVAEREALVEPDEARLAELREAIARAEAE
                     LSECAEQIRALRDEVPQLEAARGEALQQLQELQREGHTLEGRLSALQRIQAQAEQNGK
                     IQDWLEKYGLQNLPRLWQKLNVEPGWEVAVESILRERLHALELSDPDLLQRLLDDAPQ
                     SRLSVYGRESGGAPAQNHGEFRPLSALVRCADEAVRAVVEGWLHGCYAVEGMPPQTTR
                     T"
BASE COUNT          864 a         1455 c         1643 g          818 t
ORIGIN      
        1 acatcggaag ccttctgctc cgccatcagt tcgaacagcc gcttcatcgc catacatcca
       61 cctccgccgc tgcgccgttt tggcgtgtta ttcgcgcagc aattcgttga tactggtttt
      121 cgcgcgcgtc ttcgcgtcca cttgcttcac gatgacggct gcatagaggc tgtattttcc
      181 gtcggccgaa ggcaggttcc caggaactac taccgatccg gccgggacgc ggccgtagag
      241 cgtctcgccc gtgaggcggt tgtagatgcg cgtgctcatg ccgatgaata cgcccatgga
      301 aatgacggag ttttcctcga cgatcacgcc ttcgacgatt tccgaacgcg cgccgatgaa
      361 gcagttgtcc tcgatgatgg tcggattcgc ctgcagcggc tccagcacgc cgccgatgcc
      421 gaccccgccg gaaagatgca cacccttgcc gatctgcgcg caactgccca ccgtggccca
      481 ggtgtcgacc atggtgcctt cgtccacata ggcgccgatg ttgacgtagg agggcatcag
      541 caccacgttc ctgccgatgt gggcgccgcg gcgcaccatg gcgttcggca cgaccctgaa
      601 tccgccggca gcgaaatccg ctgcgccgta attggcgaac ttgcccggca ccttgtcgta
      661 gtaccgggtc gaggcgcccg gcatcacttc gttgtcgttg aggcggaagg acagcagcac
      721 ggcctttttg agccactggt gtgtttgcca gcggccgtcg attttctcgg cgacgcgcag
      781 tgcgccgcgg tcgagtccgg cgagcacttc ctcgacggcg tcgcgcagtt tttggtcggt
      841 ggaggcgggg ctgacgttcg cgcggtcttc ccacgcttgg tcgatgaggc tttgatgcgc
      901 ttgcatgatg gatcgcttgt ctggtgagag tggatcgaga cgccgcgacg cggcgtgcgg
      961 gaccggaggc ggctcagagg ccggcaagcg atggcggcgg attggggtag ccgctggcgc
     1021 agagcttgtc gaagctcagg tgcaaatggg aaagcaggaa cctggcgtca gagcgactgc
     1081 gcatgggcat tcagccgctt caggccgtcc aggcattctt tcagcggcgc gaccagcgca
     1141 acgcggatgt attgcgcgcc cggattgacg ccgtgcgcct cgcgcgccag atagctgccg
     1201 ggcaggacgg cgacgttgta gttggcgtac agcccgcgtg cgaactccgc atcgtcaatg
     1261 ggcgtacgga tccataaata aaagccgccg acgggcagtt cggtctgcag cggctcggcg
     1321 aggagttcgc gcgccgcgag gaatttctcg cgatacaggc gacggttctc ggcgacatgc
     1381 gcctcgtctc gccaggcagc ttcgctcgct tcctggatcg acgggctcat ggcactgccg
     1441 tggtaggtgc gatacagcag gaactgccgc atcagctgcg cgtcgcccgc gacgaagccg
     1501 gagcgcatgc ccggcacgtt ggagcgcttc gacaggctgg agaacacgat cagccgcgga
     1561 aatccggtgc ggcccagttt gttggccgcc tccagcgcgc cgagcggcgg ctgcttctcg
     1621 tccagataaa gctccgaata gcattcatcg gacacgatga cgaagtcgtg tcgatcggac
     1681 agttcgaaca gcgtgcgcca ctcgtccagg ctcatgaccc ggccggtcgg attgctgggc
     1741 gagcagcagt agagcaactg ggtgcgttgc cagacgctgt ccggtacctg gccgtagtcg
     1801 atggcgaagt cgttgtcgcg gtgcgcgttg acgtagtagg gcgtggcgcc ggccagcagt
     1861 gccgcgcctt catagatttg atagaaggga ttcgggcaca cgaccacggc atcgcgggtg
     1921 cggtccacca ccacctgggc gatggcgaac agcgcctcgc gcgaaccgtt gaccggcagc
     1981 acctgggtgt cggcgtcgat tttcggcagg tcgtagcgga tctgcagcca gtcggcgatg
     2041 gcctggcgca ggctgtcgga accggccgtg gccgggtagg cggccagacc ggtgagattg
     2101 tcggccagcg cgcgctggat gaaagccggc gtcgcatgtt tgggctcgcc gatggaaagg
     2161 ctgatgggcg tgtacttcga gtcgggtgtg acggccgcgt agagcttgcg cagccgctcg
     2221 aaggggtagg gctgcagttt gccgagatcg ggattcattg tcgtttttca gcggatgttg
     2281 gactgcgctt cgaagctgag ttgcgggatc gcgatttgcg gcgtccagcc ttccttgcgg
     2341 tgctccgcca ccacggtggt gaaaatcccg ccgcgcacgt agaacttggc cgtcaggcgc
     2401 atgaaacgcg gctcgagcag ttgcaccagg tcgttcagga tcttgttggt gacggcttcg
     2461 tgataggcgc cttcgttgcg gtaggaccag atatagagct tgaggctttt caactccacg
     2521 catttcctgt ccgcgacgta gtccagatac aagaccgcga aatccggctg gccggtcatc
     2581 gggcacaggc aggtgaactc cggaatttcc atatgaatca ggaaatcgcg ctcgggcgag
     2641 gggttcggaa aatgctgcag ttcggtgctg ggttgcgtgg ccatttcgcg tatttgtggc
     2701 tgaggcagtt gcggaagcga atggtattat acggcccgcc ttctaaccgc cttatggaca
     2761 agcgcggcag aggatcagct tcagtcaccg gggcctcggt gtacgcgcgc ggcgagagga
     2821 gagcgccgtg cgcccgcagc cgcaaaacgg cgcggaccgc ccgtgaggac agctaccctg
     2881 cgcgcgcaac cgtgcccatt ccaacgtgcg tctaactcaa ctgaaactcg ccgggttcaa
     2941 gtctttcgtg gacccgacca ctatccatgt tcccgggcag cacgtcggcg tcgtcggacc
     3001 caacggctgc ggcaaatcga acatcatcga cgcggtgcgc tgggtgctcg gggagtcccg
     3061 cgcctcggcc ctgcgcggcg aatcgatgca ggacgtcatc ttcaacggct cgacgctgcg
     3121 caagcccgtt gcgcgggcca gcgtggaact ggtgttcgac aacagcctcg gcaaagctgc
     3181 cgggcagtgg tcgcaatatg cggaaatctc cgtcaagcgg atcctgaccc gtgagggcga
     3241 ttcgtcctac tggatcaaca acacccaggt tcgccgtcgc gacgttcagg atatcttcct
     3301 cggcacgggc gtcggcccgc gcgcctacgc catcatcgag caggggatga tctcccgcgt
     3361 catcgaggcc aagccggaag aactgcgcgt cttcctggaa gaggcggctg gcgtgtccaa
     3421 atacaaggag cgccgcaagg agaccgaaaa ccgcctcgag gacacgcgcg aaaacttggc
     3481 gcgggtggag gacatccgcg gcgaactcga cgcgcagatc gaaaaactga cgcagcaggc
     3541 cgaaatcgcg cgccagtaca agacctatca gaccgaggca cgcgacaagc agaacctgct
     3601 ctggtacctg cgcaagcagg acgccgaagc cgatgcgcag cgcctgcagc gcgacatcga
     3661 gcaggcggtc aatgcgctgg aggccgaaac cgcccggttg cgcgaaatcg aggcgcgcct
     3721 ggaagcggtg cgggccgagc attatgccgc cggcgacgcc gtcaatgcgg cgcagggcga
     3781 gttctacggc gtcaacaccg aggtctcccg tctggagacc gaaattcgct atgtcggcga
     3841 aaagcgccag cgcctggaag cgcaggcggc gcaactggac atgcaggtcg agcaatggca
     3901 gcgtcagcgc gaggacctcg ccgatgcgct gaccatgtgg cgcgaccggc gcgaggccgc
     3961 ggccgagcgt cttgcgctcg cggaggcacg cgcagaggaa gaacaggcgc ggctgccgga
     4021 cgccgaagaa gcattccgcg ccttgcagga gcgcctgggc gaggcgcgcg acgccatcgc
     4081 gcgtgccgag caggccttcc aggtcgagca gacccatctg acgcataccg gcaggacgct
     4141 gcaacagttg gacgtgcgcg aagagcggct ggtcgcggag cgcgaagcgc tggtggaacc
     4201 ggatgaggcg cggctcgccg aattgcgcga agccattgcg cgcgccgaag ccgaactgtc
     4261 cgaatgcgcc gagcagatcc gcgcgctgcg cgacgaagtg ccgcaacttg aggcggcgcg
     4321 cggggaagcc ctgcagcagt tgcaggagtt gcagcgcgaa ggccacacgc tggaaggccg
     4381 cctctccgcg ctgcagcgca tccaggcgca ggccgagcag aacggcaaga ttcaggactg
     4441 gctggagaag tacggtttac agaacctgcc gcggctgtgg cagaagctga acgtcgagcc
     4501 cggctgggaa gtcgcggtgg agtcgatcct gcgcgagcgg ctgcatgcgc tggagctgtc
     4561 cgatccggat ctgctgcagc ggctgctgga cgacgcgccg caatcccggc tcagtgtcta
     4621 tggtcgcgaa agcggcgggg cgccggcgca gaaccacggc gaattcaggc cgctgtccgc
     4681 gctggtgcgc tgcgccgacg aggcggtgcg tgcggtcgtc gaagggtggc tgcacggctg
     4741 ttacgcggtg gagggcatgc cgccccagac gacgcgcaca
//
LOCUS       DATGOU010000086         7216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_6003153, whole genome shotgun sequence.
ACCESSION   DATGOU010000086 DATGOU010000000
VERSION     DATGOU010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7216
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6003153"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="VKZ48_05255"
     CDS             complement(<1..1537)
                     /locus_tag="VKZ48_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HLU77295.1"
                     /translation="MSPSFNKDSSAPPASPAEAPGLDRTRAALRTLLGLAIALPLAFF
                     LGYAWTSYTAARDEVKDAVERLGGVAEEHALKVFEINDQLINRILDLAERMDDAAIAG
                     AQTQYHAMLSRIGADIPQLSSINLFGSDGKLLVSNRFYPVPQIDIAGRADFQYHLERR
                     GPLYVSPVYQGAALGLPVFNVSVARRGPNDEFRGLVSVSLRPDYFSEYYKQLAQGKPG
                     VAIMLVHADGSIMARHPAVPRDVQVQSYGPVMQEIDAAKYTGVTEYVSPVDGRARFGA
                     FRQIGSYPVYIVTGYARDAWLSEWYGRTAVVAGFTFVPSLALCVLLLIALRRLQREEE
                     GWDQWRAEVTHRQAMEIAYKQARRLEALGQLTGSVAHDFNNLLMVVSTSTMLLRRRLA
                     GREDVEQPLGALERSLEAGKRLTRQLLAFSRKQPLRPEVLDVPTQMGEFIELIRASLG
                     GRISLSLDIDPETRPVYVDEGEFELAMLNLAINARDAMPEGGTLTVCARNVHLDGEGE
                     PGLR"
     gene            complement(1597..2676)
                     /gene="mnmA"
                     /locus_tag="VKZ48_05260"
     CDS             complement(1597..2676)
                     /gene="mnmA"
                     /locus_tag="VKZ48_05260"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HLU77296.1"
                     /translation="MSAKERIVVGMSGGVDSAVSALLLKRAGYEVIGLFMKNWEDDDD
                     DEYCSTRQDLIDVASVADVIGIDLEVVNFAAEYKDRVFSEFLREYSAGRTPNPDVLCN
                     AEIKFKAFLDHAMAMGARRIATGHYAGVRERDGRYELLRGADPSKDQSYFLHRLNQAQ
                     LSRVLFPLAELRKTEVRRIAQEAGLPVAAKKDSTGICFIGERPFREFLNRYLPSEPGP
                     MLTPEGRQIGEHMGLAFYTIGQRSGLGIGGARGGSGEPWYVADKDLARNALIVVQGHD
                     HPLLLRDSLTAADLAWVAGEAPDAAGVYGGKTRYRQRDAECRFTRLDDSGCELRFAEP
                     QWAVTPGQSVVLYRNEVCLGGGVIQ"
     gene            complement(2673..3128)
                     /locus_tag="VKZ48_05265"
     CDS             complement(2673..3128)
                     /locus_tag="VKZ48_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HLU77297.1"
                     /translation="MSAPAWNPRVVVAALVERDGKFLLVEEEADGRLVFNQPAGHWER
                     GETLVEACARETMEETAHEFRPTGVLGLYVWTHEGKQKTFVRVAFTGEVGAHDPVRAL
                     DKEIRRAVWLDVEEIRALAPRHRSPLVMRCIDDYLAGQRHPLSLIAHFR"
     gene            3280..4608
                     /locus_tag="VKZ48_05270"
     CDS             3280..4608
                     /locus_tag="VKZ48_05270"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HLU77298.1"
                     /translation="MSNRNTRRQSSVPTGRIERFMQLGLMAGELVIGGMIEGVRRVGT
                     APAEGAANPMLNPRTARRIADRLSHMRGAAMKLGQLLSLEGPDLLPPDFSAALGMLRA
                     GADPMPHTQLRRVLGREYGSGWEARFRHFDYDPVAAASIGQVHYAVAADGRVVALKIQ
                     YPGVAQSIDSDVENVTRLMRLLKLLPGNIDVRETVAEAKRQLHQEADYIEEAQLLQRY
                     SKLVADEPRLYVPKVHMDLTTKRILAMDFVDGEPLETLADEETSQSRRDALGRVLMRL
                     LFRELFEFRFMQTDPNFANYLYDAAENRIVLLDFGSVRRFESRFVARFLRLCKAIIAN
                     DRPAVRRLATEIGYVQYDDPEHVIERGIELIMLVCEPLRHRGPYDFAHSKLPQRAREA
                     GFDLLLSGDTLRAPPPETLFLHRKLAGSFLIMARIGARVNVRQLISAFLP"
     gene            complement(4636..5499)
                     /locus_tag="VKZ48_05275"
     CDS             complement(4636..5499)
                     /locus_tag="VKZ48_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLU77299.1"
                     /translation="MQAQIEAFYDAVTGTVSYVVYDAPQGHGAIIDPVLDYDPKSGRT
                     TTGSADRILRFVESQRLRIDWLLETHAHADHLSAAQYLKAQCGGRIAIGARITAVQEV
                     FREVFNIERGFLPDGSQFDHLFADGERFAIGALEAEVMYVPGHTPADVAYRIGDAVFV
                     GDTLFMPDVGTARCDFPGGDAHELYRSIRKLLSLPPETRLYMCHDYPPEGERDPAWET
                     TVAEQRERNIHVHDGVSEEEFVALRNARDQTLELPMLILPSVQVNIRAGQMPPAEDNG
                     KRYLKIPINAL"
     gene            5586..5894
                     /locus_tag="VKZ48_05280"
     CDS             5586..5894
                     /locus_tag="VKZ48_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLU77300.1"
                     /translation="MSDTTLDLDTMRDAATEVAGLLRTLGNADRLLLLCQLTEGEFCV
                     SELEACTGISQPTLSQQLGVLRNMALVDTRREGKNIYYRIADARALALLQTVHDLYCD
                     "
     gene            5901..6338
                     /locus_tag="VKZ48_05285"
     CDS             5901..6338
                     /locus_tag="VKZ48_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020723315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HLU77301.1"
                     /translation="MQIDWLHFTPWHALIGGALIGLAATALMLLNGRIAGISGIFAGM
                     LQPRLQDAGWRIAFIFGLMMAPAVYTAFTRAAPTIRIDADYPLLALAGLLVGLGTRYG
                     SGCTSGHGVCGLSRLSPRSLVATLSFMGAGIAVVFLVRHVLGD"
     gene            6342..6785
                     /locus_tag="VKZ48_05290"
     CDS             6342..6785
                     /locus_tag="VKZ48_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HLU77302.1"
                     /translation="MTARPYVCAFATGLLFGIGLLIAGMADPHKILAFLDLAGLWDPS
                     LGLVMVGAIAMGSIGFTFAQRRKKTLLGGTMEWPTARHIDRRLVLGSLAFGIGWGLSG
                     FCPGPALIATAAGHSKALVFTVFMVIGMTAYRMIERRRTLAAPSP"
     gene            complement(6919..>7216)
                     /locus_tag="VKZ48_05295"
     CDS             complement(6919..>7216)
                     /locus_tag="VKZ48_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77303.1"
                     /translation="FTVVPLGEADMSDSISLLLLTLLSLQAGPTNAAENDARMTANAS
                     TRCAAVAPAAKTSDADPACNRTAAQRQRAVRVTIPASTRARAEAKPITVSASAR"
BASE COUNT         1232 a         2441 c         2290 g         1253 t
ORIGIN      
        1 cgcgcaggcc cggctcgccc tcgccgtcca gatgcacgtt gcgggcacag acggtgagcg
       61 tgccgccctc gggcatcgca tcgcgcgcat tgatggccag gttgagcatc gccagttcga
      121 actcgccctc gtccacatag accgggcggg tctcaggatc gatatcgagc gacagcgaaa
      181 tgcgtccgcc cagcgaggcg cggatgagtt cgatgaattc gcccatctgc gtgggcacgt
      241 cgagcacctc gggccgcaac ggctgcttgc gcgagaaggc gagcagttga cgcgtcagcc
      301 gcttgcccgc ttccagcgaa cgctccagcg ccccgagcgg ctgttcgacg tcttcgcgcc
      361 ccgccaaccg gcggcgcagc agcatggtgc tggtcgagac caccatcagc agattgttga
      421 agtcgtgcgc gacgctgccg gtcagttgac cgagcgcctc cagccggcgc gcctgcttgt
      481 aggcgatttc catcgcctgg cgatgcgtca cttccgcgcg ccattgatcc cagccttcct
      541 cctcgcgctg cagccgccgc agcgcgatca gcagcagcac gcacaaggcc agactcggca
      601 cgaaagtgaa gccggccacc acggcggtgc gcccatacca ctccgacagc cacgcgtcgc
      661 gcgcataacc ggtgacgatg tagacggggt agctgccgat ctgccggaag gcgccgaagc
      721 gggcgcgtcc gtccacaggc gacacatact cggtcacgcc ggtgtatttg gcggcatcga
      781 tttcctgcat gaccggcccg taagattgca cttgcacgtc gcgcggtacg gccggatggc
      841 gcgccatgat gctgccgtcg gcatgcacca gcatgatcgc cacgcccggc ttgccctgcg
      901 caagctgctt gtagtactcg ctgaaataat cgggacgcag cgaaacgctg accaagccgc
      961 ggaactcgtc gttcggtccg cgccgcgcca cgctgacgtt gaagaccggc aagccgagtg
     1021 cagcgccctg atagacgggc gaaacgtaga gcggcccgcg ccgctcgagg tgatactgaa
     1081 aatctgcgcg tccggcgata tcgatctgcg gcacgggata gaagcggttg ctgacgagca
     1141 gcttgccgtc gctgccgaac aggttgatcg acgacagttg cggaatatcg gcgccgatgc
     1201 gcgacagcat cgcgtgatac tgcgtttgcg cgccggcgat cgcggcatcg tccatccgct
     1261 cggccaggtc gaggatgcgg ttgattaact gatcgttgat ctcgaagacc ttgagcgcgt
     1321 gttcttccgc caccccgccc aggcgctcaa cggcgtcctt cacctcatcg cgcgcggcgg
     1381 tgtagctggt ccaggcgtac ccgaggaaga aagccagcgg caacgcaatg gccagaccca
     1441 gcagcgtgcg cagggcggcg cgcgtgcgat ccagaccggg tgcctcggca ggcgatgcgg
     1501 gcggcgcgct gctgtccttg ttgaacgagg gactcacaac ttctcctccc ctccatagtc
     1561 gtccggcgca tatcgccggg ggcacccgcg ataccgctat tgaataacgc caccgcccag
     1621 gcaaacttcg ttccggtaca gcaccacgga ctgccccggc gtcaccgccc attgcggctc
     1681 ggcgaagcgc aactcgcacc cgctgtcgtc cagccgcgtg aagcggcatt ccgcatcgcg
     1741 ctgccgatag cgcgtcttcc cgccgtaaac ccccgccgca tcgggcgcct caccggccac
     1801 ccaggccaga tcggcggccg tcaggctatc ccgcagcagc agcggatgat cgtgcccctg
     1861 cacgacaatc agcgcattgc gcgccagatc cttgtcggcg acgtaccagg gttcgccgct
     1921 gccgccgcgc gccccgccga tgcccaggcc cgagcgctgg ccgatggtgt aaaacgcaag
     1981 acccatgtgc tcgccgatct gcctgccctc cggcgtgagc atcggacccg gctcgctcgg
     2041 caggtagcga ttgaggaatt cgcggaacgg ccgctcaccg atgaagcaga tgccggtcga
     2101 atccttttta gccgccaccg gcagaccggc ttcctgcgcg atcctgcgca cttctgtctt
     2161 gcgcagctcg gccagcggaa agagcacgcg cgacaactgc gcctgattca agcggtgcag
     2221 gaaatagctc tgatccttgg acggatcggc gccgcgcagt agctcgtacc gcccgtcgcg
     2281 ttcgcgcacg ccggcgtagt ggcccgtggc gatgcgccgt gcgcccatgg ccatggcgtg
     2341 atcgagaaag gccttgaact tgatttcggc attgcacagc acgtccggat tgggcgtgcg
     2401 ccccgccgag tattcgcgca ggaattcgct gaacacgcga tccttgtact cggcggcgaa
     2461 gttcaccact tcgagatcga tgccgataac gtcggccacc gatgccacgt cgatgaggtc
     2521 ctgacgggtg gagcagtact cgtcatcgtc gtcgtcttcc cagttcttca tgaacaggcc
     2581 gatgacttca tagcccgcac gcttgagcag cagcgccgag acggccgaat ccacgccgcc
     2641 cgacatcccc acgacaatgc gttccttcgc gctcatcgga aatgcgcgat cagcgacagc
     2701 gggtgacgct gtccggcgag atagtcgtcg atgcagcgca tcaccagcgg actgcgatgg
     2761 cgcggcgcga gtgcgcggat ctcttccacg tcgagccaca ctgcgcggcg gatttccttg
     2821 tcgagcgcgc gcaccggatc gtgcgcgccg acttccccgg tgaacgcgac gcgcacgaaa
     2881 gttttctgct tgccttcgtg cgtccacacg taaaggccga gcacgccggt cgggcggaac
     2941 tcgtgcgccg tctcttccat ggtttcgcgc gcacaggcct cgaccagcgt ttcgccccgc
     3001 tcccaatgac ccgcaggctg gttgaagacc agacggccgt ccgcctcctc ctcgacgagc
     3061 aggaacttgc cgtcgcgctc cacgagcgcg gcgacgacga cgcggggatt ccaggcaggg
     3121 gcactcattt ctcgatcgca aagacgcgca tgcaggcgcg aagagcgcat tctagcgcgt
     3181 cgcccgcggc gaaaccgcgg gcactgcgct tgctccccag tgtgcgttcc gcgttgccag
     3241 ggcgccgcgg ccatgattcg cagaaagaag gaacctcagg tgtccaatcg caacacacgc
     3301 cggcaatcca gcgtccccac cggtcgtatc gaacgcttca tgcagctcgg cctgatggcg
     3361 ggcgaactgg tgatcggcgg catgatcgaa ggcgtgcgcc gcgtcggcac cgcgccggcc
     3421 gaaggcgcgg ccaatcccat gctcaatccc cgcacggccc ggcgcatcgc cgaccggctg
     3481 tcgcatatgc gcggcgcggc gatgaagctg gggcagttgc tgtcgctgga aggacccgat
     3541 ctcctgccgc ccgacttttc cgccgcgctc ggcatgctgc gcgcgggtgc cgacccgatg
     3601 ccgcacaccc agttgcgccg cgtgctgggg cgcgaatacg gcagcggctg ggaagcgcgc
     3661 ttccggcact tcgattacga cccggtggcc gccgcctcca tcggccaggt gcattacgcg
     3721 gtcgccgcag acggccgcgt cgtcgcgctg aagattcagt atccgggcgt tgcgcaatcg
     3781 atcgacagcg atgtggagaa cgtcacccgg ctgatgcgcc tgctcaagct gttgccgggc
     3841 aacatcgacg tgcgcgaaac ggtggccgaa gccaagcgcc agctgcatca ggaagccgac
     3901 tacatcgagg aagcgcaact gctgcagcgc tacagcaagc tggtggccga cgaaccgcgc
     3961 ctgtatgtgc cgaaggtgca catggatctc accaccaagc gcattctcgc catggacttc
     4021 gtcgacggcg agccgctgga gacgctggcc gacgaggaaa cgtcgcaaag ccggcgcgac
     4081 gccctgggcc gcgtactgat gcggctgctg ttccgcgagc tgttcgagtt ccgcttcatg
     4141 cagacggacc cgaacttcgc caactacctc tacgacgccg ccgagaaccg catcgtgctg
     4201 ctcgacttcg gctcggtgcg gcgcttcgag agccgcttcg tcgcgcgctt cctgcggctg
     4261 tgcaaggcga tcatcgccaa cgaccggccg gcggtgcggc gtctggcgac cgaaatcggc
     4321 tacgtgcaat acgacgatcc ggagcatgtg atcgagcggg gcatcgaact catcatgctg
     4381 gtgtgcgagc cgctacgtca ccggggtcct tacgatttcg cgcactccaa gctgccgcag
     4441 cgcgctcgcg aagccggctt cgatctgctg ctttctggcg atacgctgcg cgcaccgccg
     4501 cccgagacgc tgttcctgca tcgcaagctg gccggctcct tcctcatcat ggcgcgcatc
     4561 ggcgcacgcg tgaacgtgcg gcaactcatc agcgcatttc tgccctaagg cacgcaatcc
     4621 aaacttcccc cacacctaca gcgcgttgat gggaatcttc agatagcgtt tgccgttgtc
     4681 ctcggccggc ggcatctgtc cggcgcggat gttgacctgc accgaaggca ggatgagcat
     4741 cggcaattcg agcgtctggt cgcgtgcgtt gcgcagcgcg acgaactcct cttcgctgac
     4801 gccgtcgtgg acgtgaatgt tgcgttcgcg ctgttcggcc accgtcgtct cccatgcggg
     4861 gtcgcgctcg ccttcgggcg gatagtcgtg gcacatgtag aggcgcgtct ccggcggcag
     4921 cgacagcagc ttgcggatgg aacgatagag ctcgtgcgca tcgccgcccg ggaaatcgca
     4981 gcgcgccgtg cccacgtcgg gcatgaacag cgtatcgccc acgaacaccg catcgccgat
     5041 gcgataagcc acatccgccg gcgtatgacc cggcacgtac atcacttccg cctcgagcgc
     5101 gccgatggcg aagcgctcgc cgtcggcgaa cagatgatcg aactgcgagc cgtcgggcag
     5161 aaaaccgcgc tcgatgttga acacctcgcg gaatacctcc tgcaccgcag tgatcctcgc
     5221 accgatggcg atgcgcccgc cacactgcgc cttgagatat tgcgcggcgc tcaagtgatc
     5281 ggcatgcgca tgcgtctcca gcagccagtc gatgcgcaga cgctgcgact cgacgaaacg
     5341 cagaatgcgg tcggcggagc cggtggtggt gcgcccggac ttggggtcgt agtcgagcac
     5401 cggatcgatg atggcgccat gcccttgcgg tgcgtcgtac accacataac tcacggtgcc
     5461 tgtcacggca tcgtagaagg cttcgatttg cgcttgcatg acggcatcct ccgatgcgaa
     5521 acagcttgcg ggaacgatat atttttttat atattgacgc aaccgctttc gcacccatcg
     5581 gcacaatgag cgacacgacg ctggatctgg acaccatgcg cgacgcggcg accgaagtcg
     5641 ccggactgtt gcgcacgctc ggcaacgccg atcgcctgct gctgttgtgt cagctcaccg
     5701 aaggcgagtt ctgcgtgagc gaactggaag catgcaccgg catctcccag ccgaccctgt
     5761 cacaacagct cggggtgctg cgcaacatgg cgctggtgga cacgcgccgc gaaggcaaga
     5821 acatctacta ccgcattgcc gatgcacgtg cgctcgcttt gctgcaaacg gtccacgacc
     5881 tttattgcga ctaagacaca atgcagatcg actggctcca tttcacgccc tggcatgcgc
     5941 tgatcggcgg cgcattgatt ggccttgcgg ccactgcact catgctgctc aacggccgca
     6001 tcgccggcat cagcggcatc ttcgccggca tgctgcaacc gcgactgcag gacgcgggct
     6061 ggcgcatcgc gttcatcttc gggctgatga tggcgccggc cgtatacacc gcgttcacgc
     6121 gcgccgcgcc cacgatccgc atcgatgcgg actacccgtt gctcgcgctc gccggactgc
     6181 tcgtcggcct aggcacccgc tacggctcgg gttgcaccag cggccacggc gtgtgcgggc
     6241 tgtcacgcct gtcgccacgt tcgctcgtgg cgaccttgtc gttcatgggg gccggcattg
     6301 cggtggtctt tctcgtgcgc cacgtgctgg gagactgaac gatgaccgcg cgtccctacg
     6361 tctgcgcctt cgccaccggc ctgctgttcg gcatcggcct gctgatcgcc ggcatggccg
     6421 acccgcacaa gatcctcgcc ttcctcgatc tggccggcct gtgggacccg tccctggggc
     6481 tggtgatggt cggcgccatc gctatgggca gcatcggttt cactttcgca caacgaagga
     6541 agaagacgct gctcggcggc acgatggaat ggccgacggc gcgacacatc gatcgacgcc
     6601 tcgtgctcgg cagtctggct ttcggtatcg gctggggttt gtccggtttc tgtcccggcc
     6661 cggcgctgat cgccactgcg gcaggtcaca gcaaggcgct ggtgttcacc gtcttcatgg
     6721 tgatcggcat gaccgcctac cgcatgatcg agcggcggcg cactctcgcc gcgccctcgc
     6781 cctagattcc gtaaacgcaa tcgcccatcg gcatcggatg cctggtggcg gatacgacga
     6841 cggcccggga ttcgggccgt cggtgaaaca ctggagaacg aagccgccga tcgctgcggc
     6901 tttgaaacgg cggacggttc agcgtgcgga tgcggagacg gtgatgggtt tcgcctccgc
     6961 ccgcgcacgg gtggacgccg ggatggtgac gcgcaccgcg cgttggcgtt gcgcggccgt
     7021 cctgttgcag gccggatcgg cgtccgaggt tttcgccgcc ggcgcgacgg ccgcgcagcg
     7081 cgtcgacgca ttcgccgtca tgcgcgcatc gttttcggcg gcgttggtcg gcccggcttg
     7141 cagggaaagc agggtcaaca gcagcagact gatggaatcg ctcatgtcgg cctcaccaag
     7201 tggaactacg gtgaag
//
LOCUS       DATGOU010000087        10704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3343311, whole genome shotgun sequence.
ACCESSION   DATGOU010000087 DATGOU010000000
VERSION     DATGOU010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10704
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3343311"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..1229
                     /locus_tag="VKZ48_05300"
     CDS             36..1229
                     /locus_tag="VKZ48_05300"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HLU77304.1"
                     /translation="MPILKMTDLDLRDKRVLVRADLNVPQDDSGAISDDTRIRASLPA
                     IRHALKAGARLMVTSHLGRPKEGEYKPADSLAPVAQRLSELLGVSVPLKQNWLDAGEA
                     APAAGEVVLLENCRLNKGEKKNDEALAQRIAALCDVYVNDAFGTAHRAEATTHGVAKY
                     APVACAGPLMAAEIEALTKALRDPARPLVAIVGGAKVSTKLTILAALAEKVDQLIVGG
                     GIANTFMLAAGLPIGKSLAEPDLVDEARKIIEMLRAKGGDVPIPVDVVCAKTFAADAA
                     AEVKASEAVAADDLILDIGPKTAAAWAEILAKAGTIVWNGPVGVFEFPQFGEGTKVLA
                     QAIAGSKAFSLAGGGDTLAAIAKFGVTDRISYISTGGGAFLEFLEGKKLPAIEILEQR
                     AAAQA"
     gene            1321..2799
                     /gene="pyk"
                     /locus_tag="VKZ48_05305"
     CDS             1321..2799
                     /gene="pyk"
                     /locus_tag="VKZ48_05305"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HLU77305.1"
                     /translation="MLSQQNNYPFGSDKPRSTKIVATLGPASSDVDTLERMFRAGVDV
                     VRMNFSHGTAEDHLKRAETVREACRRSGRTVGIMADLQGPKIRVGKFKDGRVTLQAGQ
                     TFILDSNCELGDETRSGLDYKELPRDVQPGDVLLLDDGKIVFDVEKVEGVEIHSRVRH
                     GGVLSNNKGINRVGGGLTAPTLTAKDIEDIRIAAQIRADYLAVSFPKCGKDMYTAREM
                     LRAAGGEALLIAKIERAEAVELAAFEDILSGSDGIMVARGDLAVEVGDAAVPALQKRM
                     IQLARERNKLTITATQMMESMIHSPVPTRAEVSDVANAVLDGTDAVMTSAETAAGKYP
                     VETIAAMSRICMEAEKSTRVMLDRDFLDRVFTRVDQSIAMAAVFTAYHLKVKAIAALT
                     QSGSTALWMSRLNVGVPIYALTSETTTRYRVSLFRDVCPLTVNYQGHDREALLKEAEE
                     VLVQNSVVEKGDLIVITFGEPIGKSGGTNTMKIVRVGEQRAP"
     gene            3279..4298
                     /locus_tag="VKZ48_05310"
     CDS             3279..4298
                     /locus_tag="VKZ48_05310"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017331407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HLU77306.1"
                     /translation="MSQSLAAVAQAMVAHGKGILAADESSGTIEKRFKTINLESTEEH
                     RRAYRDLLFSAKGVGEYISGVILFDETLRQKSADGTPFAELLTRNGMIPGIKVDAGAK
                     PMALCPGETITEGLDGLAKRCQEYVKLGAKFAKWRAVIDIGEGIPSTAAIIANAHALA
                     RYAAICQENGLVPIVEPEVLMDGAHTIDRCEEVTLAVLHEVFDALVSQRVVFEDMVLK
                     PNMVVSGKKCPQQAGPQEVAERTLQTLKRTVPAAVPGIAFLSGGQSDELATEHLSIMN
                     QLGGGPWALTFSYGRALQAAALKAWSGNPANVAAAQAALLHRARMNSLAAKGEYKPEL
                     ERQAA"
     gene            4368..5261
                     /locus_tag="VKZ48_05315"
     CDS             4368..5261
                     /locus_tag="VKZ48_05315"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HLU77307.1"
                     /translation="MAALLQSDIKSLQFLHRGKVRDLYAVGEDKLLVIQTDRLSAFDV
                     ILPQPIPGKGQVLTAMSNFWFKKLADIIPNHMTDIDPLSVVTPEERDQVAGRAIVVRR
                     LKPLKVEAVARGYLIGSGWKDYVRTGKLCGIALPAGLREAERLPEPLFTPSTKADAGQ
                     HDENISYEEAERIIGADYARQVRDATLALYSAAAAYAATKGIIIADTKFEFGTDEQGT
                     VHLMDEALTPDSSRFWDAAQYQVGISPPSYDKQIVRNWLEQSGWNKQPPAPQVPEEVL
                     LRTAERYNEVLEKLTGSRIAL"
     gene            5330..6301
                     /locus_tag="VKZ48_05320"
     CDS             5330..6301
                     /locus_tag="VKZ48_05320"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HLU77308.1"
                     /translation="MVEPNEEGIARAVAALRAGELVAFPTETVYGLGADAANAEAVRR
                     IFKLKGRPADHPVIVHIGDAAQLDDYASEVPPEARRLAERFWPGPLTLILKRAAHVPD
                     IVTGGQDTVGLRVPAHPVARTLLEAFGGGIAAPSANKFGRISPTTAAHVAADYGQHAP
                     LILDGEAASVGIESTIVDLSGETPRLLRPGAITAAQLAEVLGSPPADPDADAPRAPGS
                     LDKHYAPKAALRVVKRVEMIEILSTNRGRRVAVLALEVNVPRLAAHLVRVAPAVAAQY
                     AQSLYANLRALDATGADLILVEQPPQTAAWAAVNDRLKRAAKSAEAT"
     gene            complement(6298..7026)
                     /locus_tag="VKZ48_05325"
     CDS             complement(6298..7026)
                     /locus_tag="VKZ48_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77309.1"
                     /translation="MDRRSFLVSAGALALAGCAIRPAPRGVYELTEVPAPTYRVGDRW
                     IYKRTDGYNGLPRGTLTREVRAVEENGIRIVTQDEHGHILDDARYEAPGLQLAGTFTE
                     DGPMFGRCQTPFREYDFPLVAGKEWRDAFYLDRDDPGGIRNYVTASLYAEGWEDIQVA
                     GQSFHALVLRRSLNLGEKSFWYGTMYRYETEWYVPALRSFARMETTEEYYDRSGGGRR
                     GGGGGGLRNGDRFLWELESFRLAA"
     gene            7149..7224
                     /locus_tag="VKZ48_05330"
     tRNA            7149..7224
                     /locus_tag="VKZ48_05330"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7182..7184,aa:Ala,seq:cgc)
     gene            7322..7741
                     /locus_tag="VKZ48_05335"
     CDS             7322..7741
                     /locus_tag="VKZ48_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HLU77310.1"
                     /translation="MNASPTHDRWRRLWLLCGWVMIGLVILFSLIPAPPILDVSQGDK
                     LQHLGGYALLMAWFAQVYRRPGSRMCVALGLTALGITLEVLQGMTGWRSFESSDMVAN
                     TAGVLLGWLLAPPRGPELYRRVADRDRLAANAQSSAR"
     gene            complement(7723..8511)
                     /gene="pcaD"
                     /locus_tag="VKZ48_05340"
     CDS             complement(7723..8511)
                     /gene="pcaD"
                     /locus_tag="VKZ48_05340"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HLU77311.1"
                     /translation="MNKVRANGIDLHYRIEGQGPWIVMSHALACDHRMWDAQAAQLAQ
                     RYTVLRYDTRGHGASDAPPGPYSFDMLAADAAALFDALGIERAHWLGLSMGGMIGQAF
                     ALAYPQRVASLVLADTSSRYPADVETVWAERIRTVRDQGMEAVVSATLERWFTKPFRD
                     AHPEVMQRIGDTIRATPVEGYAGCGQAIAKLNFTEALRSLRCPTLVIVGDQDSGTPIE
                     MAREIHEAIEGSRLAVIASAAHLSNIEQAEAFNGLVEDFYRALD"
     gene            complement(8578..>10704)
                     /locus_tag="VKZ48_05345"
     CDS             complement(8578..>10704)
                     /locus_tag="VKZ48_05345"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HLU77312.1"
                     /translation="GADMSAVPALNELKLNLAGYLKPADLSRIEEAYRFSDAAHQGQF
                     RKSGEPYISHPIAVAEILSEWQLDPQTLMAALLHDVVEDTKVSKDDISERFGPAVAEL
                     VDGVSKLDKIEFQSQKDAQAENFRKMMLAMARDVRVILIKLADRLHNMRTLDAVGMHK
                     RHRIARETIEIYAPIASRLGLDSLYRELQELSFKHLYPLRYRTIAKAVKAARGNRREV
                     VNKILEAITKKLADEGIEASITGREKGIYSIYKKMLEKSLSFSEVLDIYGFRVVVKDV
                     PSCYLALGALHSLYKPVPGKFKDYIAIPKANGYQSLHTALIGPFGTPVEVQIRSREMH
                     HIAETGVASHWLYKEEEAATSEVQRRTHNWLQSLVEIQSASGDSAEFLEHVKVDLFPD
                     EVYVFTPAGKIMSLPRGATPVDFAYAVHTDIGNRCVAAKVNYELAPLRTELRNGDRVE
                     IITAAHAHPNPAWLSYVKTGKARSQIRHFLKTVQFEESVSLGERLLNQTLRSLDADLA
                     DIASEQWERLVRESGAKSREEVLADIGLGKRLAAIVARQLLALTDQTADTKPAGSIVI
                     RGSEGIAVQFGKCCRPIPGDPIMGFIKKGQGLVVHTTDCPVAAKSRTDPDKWLDVEWE
                     ADPSKLFDVCVRVVAANQRGVLAKVAAAIAEAGSNIDNVSMDEERGLYTTMLFTIQVR
                     NRLHLARVMKAVRRVHDVVRLSRVKE"
BASE COUNT         1918 a         3395 c         3506 g         1885 t
ORIGIN      
        1 cgggcggcga cggagagcat ggaagcgaaa cgatcatgcc gatattgaaa atgaccgatc
       61 tcgatctgcg agacaagcgg gtgctcgtgc gcgcggatct caacgtgccg caggacgaca
      121 gcggcgcgat tagcgacgac acgcgcatcc gcgcctcgct gccggcgatc cggcacgcct
      181 tgaaggccgg cgcccgcctg atggtgacct cccatctggg ccgaccgaag gaaggcgagt
      241 acaagccggc cgattcgctg gccccggtcg cgcaacgtct ctccgaactg ttgggcgtct
      301 cagtgccgct caagcagaac tggctggatg cgggcgaggc ggcgccggcg gcgggcgaag
      361 tcgtgctgct ggaaaactgc cggctcaaca agggcgagaa gaagaacgac gaagcgttgg
      421 cacagcgcat tgcggcattg tgcgatgtgt atgtcaacga cgcgttcggc acagcgcatc
      481 gcgccgaagc gaccacccac ggggttgcca aatacgcgcc ggtcgcctgc gccggcccgc
      541 tcatggcggc cgagatcgaa gcgctcacca aagcgctgcg cgatcccgcg cggccgctgg
      601 tggcgatcgt cggcggcgcg aaggtctcca ccaagctcac cattctcgct gcgttggcgg
      661 agaaggtcga tcaactgatc gtaggcggcg gcattgccaa caccttcatg ctcgccgcgg
      721 gtctgcccat cggcaagtcg ttggccgaac cggacctggt ggacgaagcg cgcaagatta
      781 tcgagatgct gcgcgcgaag ggcggcgatg tgccgattcc cgtcgatgtt gtgtgcgcaa
      841 aaacgtttgc agccgatgca gcggcagagg tgaaagccag cgaggcggtc gccgcggacg
      901 acctgatcct ggacatcggg cccaaaaccg cagccgcttg ggcggaaatt ctcgccaaag
      961 caggcacaat tgtttggaac gggccggtcg gcgtgtttga attcccgcag ttcggcgaag
     1021 gcaccaaggt attggctcag gccattgccg gcagcaaggc attctcgctg gccggcggtg
     1081 gcgacactct ggcagcgatc gcgaaattcg gtgtcaccga ccgcatttcc tatatctcca
     1141 ccggaggcgg cgcgttcctg gaatttctcg aaggcaagaa attgccggcg atcgaaatcc
     1201 tggaacagcg ggccgctgca caggcctaag aggacggaat gaccggcgcc agctcactcg
     1261 gggacggtgc gcgtcgttgc gaatccggat gattatttaa agaagattga tcgaaaagac
     1321 atgctttctc agcaaaacaa ctatcccttc gggtcggaca agccgcgcag taccaagatc
     1381 gtggcgactt tgggccccgc atccagcgac gtcgacactc tcgagcgcat gttccgcgcc
     1441 ggcgtcgacg tggtgcggat gaacttctcc cacggcaccg ccgaagacca tctcaagcgc
     1501 gccgaaacgg tgcgcgaagc atgccgtcgc tcaggccgca cggtcggcat catggccgac
     1561 ttgcagggcc cgaaaatccg ggtgggcaaa ttcaaggatg gccgcgtcac cctgcaggcc
     1621 ggccagacgt tcattctcga ttcgaactgc gaactgggcg acgagacccg ctcgggcctc
     1681 gattacaagg aactgccccg cgatgtgcag cccggcgatg tgctcctgct cgacgacggc
     1741 aagatcgtgt tcgacgtgga gaaggtcgag ggcgtcgaaa tccattcgcg cgtgcgccat
     1801 ggcggcgtgc tgtcgaacaa caagggcatc aaccgcgtgg gcggtggcct gacggcgccg
     1861 acgttgaccg ccaaggacat cgaagacatc cgcatcgccg cacaaatccg cgcggactat
     1921 ctcgcggtgt cgttccccaa gtgcggcaag gacatgtata ccgcgcgcga aatgctgcgc
     1981 gccgcgggcg gcgaggcgct gctgatcgcc aagatcgaac gggcggaagc ggtggaactg
     2041 gcggcgttcg aggatattct gtccggctcg gacggcatca tggtggcgcg cggcgacctc
     2101 gcggtcgaag tgggcgatgc ggccgtgccg gcgctgcaga agcgcatgat ccaactggcg
     2161 cgcgaacgca ataagctgac catcaccgcg acccagatga tggaatcgat gatccacagc
     2221 ccggtgccga cgcgcgccga agtgtccgac gtggccaacg ccgtgctcga cggtacggac
     2281 gcggtaatga cctccgccga gacggcggcc ggcaagtatc cggtggaaac catcgccgcg
     2341 atgagccgca tctgcatgga ggcggagaaa tcgacccgcg tcatgctgga tcgggatttt
     2401 ctggaccgcg tcttcacccg cgtcgatcag tccatcgcca tggccgccgt gttcaccgcc
     2461 tatcacctga aggtgaaggc tattgcggcg ttgacgcaat cgggctccac agcgctgtgg
     2521 atgtcgcgcc tcaacgtggg cgtgccgatc tacgcgctga cctcggagac cacgacgcgc
     2581 tatcgggtca gcctgttccg cgatgtctgc ccgttgacgg tcaactacca ggggcatgac
     2641 cgcgaggcgt tgctgaaaga ggccgaggaa gtgctggtgc agaacagcgt ggtggagaag
     2701 ggtgacctga tcgtcatcac cttcggcgag ccgatcggca agtcgggcgg caccaacacg
     2761 atgaaaatcg tgcgtgtggg cgagcagcgc gcgccctgag tgcaaatatt cgtcagacca
     2821 aaacgcctcg caatgcgagg cgttttcgtt tttgccgcgc gaagcgcgtt tgttgagcgc
     2881 ctcagctgcg cagcttgacg atgaccagca ccaccagtgc ggcggccagt acggccaggt
     2941 agatatccag ccaggacata ttcctccctc cctcgcgtta tgcgtcgcgg cagcccgggc
     3001 gctgcgcggc ggcgacgcct tatgtcggca tgcgcccgct actttaacgc agcttggcgc
     3061 ggatcgctgc ggtttgagcg ctggacgcat gattcttgct cggtgccggc gcgacaaggg
     3121 cgcgccgcta cagcgagtcg agggatcgag taaaattacc gcttttcgcg caccctgttg
     3181 ccattcgctt cgatgccgcg cttcgcgggt cggggcgcca catcgatagg aacacaaggg
     3241 gcgcgcccct tgtccggatt ttctttagga gtcgcatcat gagccaatca ctcgcagccg
     3301 tggcccaagc catggtcgct cacggcaagg gcatactggc cgccgatgaa tcgtcgggca
     3361 cgatcgaaaa acgcttcaaa accatcaatc tggaaagcac ggaagagcat cgccgcgcct
     3421 accgggatct gctgttcagc gccaagggcg tgggtgaata catttccggc gtcatcctgt
     3481 tcgacgagac gctgcggcag aaatcggccg acggcacgcc cttcgccgaa ctgctcaccc
     3541 gcaacggcat gattccgggc atcaaggtcg atgccggcgc caagccgatg gcgctgtgcc
     3601 ccggggagac catcaccgaa ggtctggacg gtctggccaa gcgctgccag gagtacgtca
     3661 aactcggcgc gaagttcgcc aagtggcgcg cggtcatcga catcggcgaa ggcattccca
     3721 gcactgccgc gatcatcgcc aatgcgcatg cgctggcccg ctacgctgcc atctgccagg
     3781 agaacggcct ggtcccgatc gtcgagccgg aggtgctgat ggacggcgcg cacaccatcg
     3841 accgctgcga ggaagtgacg ctggcggtgc tgcatgaggt gttcgatgcg ctcgtcagtc
     3901 agcgcgtggt gttcgaagac atggtgctga aacccaacat ggtggtttcc ggcaagaaat
     3961 gtccgcagca ggcgggtccg caggaagtgg cggagcgcac gctgcagacg ctcaagcgca
     4021 ccgtgccggc cgccgtgccg ggcatcgcgt tcctgtccgg cgggcagagc gatgaactgg
     4081 cgaccgagca tctgagcatc atgaaccagc tcggcggcgg cccctgggcc ctgaccttct
     4141 cctacggccg ggcgcttcaa gccgctgcgc tcaaggcctg gagcggcaat ccggccaacg
     4201 tcgctgccgc gcaggcggcg ctgctgcatc gggcacgcat gaacagcctc gccgccaaag
     4261 gcgagtacaa gcccgaactg gagcgccagg cggcttgatc ccgaagggtg cgggcggatc
     4321 acgcccgcac ccctgtcctt tcagacgaac gccacccact cacgtcaatg gccgcactgc
     4381 tgcaatccga tatcaagagc ctgcaattcc ttcatcgcgg caaggtgcgc gatctttatg
     4441 ccgtgggcga ggacaagctg ctcgtcatcc agaccgatcg cctgtccgcc ttcgatgtga
     4501 tcctgccgca gcccattccc ggcaagggac aggtgctgac ggcgatgtcg aacttctggt
     4561 tcaagaaact cgccgacatc attcccaatc acatgacgga catcgatccg ctctccgtcg
     4621 tcacgcccga ggagcgcgac caggtggcgg ggcgggcgat cgtcgtgcgc cggctcaagc
     4681 cgctcaaggt cgaggcggtg gcgcggggct atctgatcgg ctccggctgg aaggactatg
     4741 tgcggaccgg caagctgtgc ggcatcgcgc tgccggccgg gttgcgcgag gccgagcgtc
     4801 tgcccgaacc cctgttcacg ccttccacca aggcggatgc cggccagcac gacgagaaca
     4861 tcagctacga ggaagccgag cggatcatcg gtgccgatta cgcccggcag gtgcgcgacg
     4921 ccacactggc gctgtacagc gcggcggcgg cctatgcggc gaccaagggc atcatcatcg
     4981 ccgacaccaa gttcgaattc ggcaccgacg agcagggtac agtgcatctg atggacgagg
     5041 cgctcacacc ggattcgtcg cgattctggg acgccgcgca gtatcaggtc ggcatcagtc
     5101 cgccttccta cgacaagcag atcgtgcgca actggctgga gcaaagcggc tggaacaagc
     5161 agccaccggc gccgcaggtg ccggaggaag tgctgctgcg cacggcggag cggtacaacg
     5221 aagtgctgga aaagctgacc ggctcgagga tcgcgttgtg agcgaactgg aatccgccaa
     5281 cgcgcccgag acgaccacca cggcgcctca gtccgcgccg ccttctacca tcgtcgaacc
     5341 gaatgaggag ggtattgcgc gcgccgtcgc cgccttgcgg gccggcgaac tggtcgcttt
     5401 ccccaccgag acggtctacg gcctgggggc ggacgcagcc aatgccgaag cggtgcgtcg
     5461 gattttcaag ctcaagggcc gtccggcgga ccaccccgtc atcgtgcata tcggcgatgc
     5521 ggcgcaactg gacgactacg caagtgaagt gccgcccgag gcgcgccgcc tggccgagcg
     5581 tttctggccg ggtccgctga cgctcatcct caagcgcgcc gcacatgtcc cggacatcgt
     5641 cacgggcggc caggataccg tcggcctgcg agtgcctgcg catccggtgg cccgcacgct
     5701 gctggaagcc ttcggtggcg gaatcgccgc gccatccgcg aacaagttcg gccgaatcag
     5761 ccccactacc gccgcgcatg tggccgccga ttacggtcag cacgcgcccc tgattctcga
     5821 cggcgaggcg gcaagtgtgg ggatagagtc gaccatcgtc gatctgtcgg gcgagactcc
     5881 gcggctgctg cgtccggggg cgatcaccgc ggcgcaactg gccgaggtac tgggttcacc
     5941 gcccgccgat cccgatgcag atgcgccgcg cgcgccgggc agcctcgaca agcattacgc
     6001 gcccaaggcg gcgctacgcg tggtcaagcg cgtggagatg atcgaaatcc tttcgaccaa
     6061 tcgcgggcgg cgcgttgccg tgctcgcgct ggaagtgaat gtgccgcgcc tggcggcgca
     6121 tctggtgcgc gtggccccgg cggtcgccgc gcagtatgcg caatcgctat atgcgaacct
     6181 gcgcgcactg gatgccaccg gcgccgacct cattctggtc gagcagccgc cccagactgc
     6241 cgcgtgggcg gcggtcaatg atcgcctgaa gcgcgcggcc aagagcgccg aggcgactta
     6301 agccgccaac cggaaggact ccagttccca gaggaagcgg tcgccgttgc ggagcccacc
     6361 gccaccgccg ccgcgtctcc caccgccgct ccggtcgtaa tattcctcgg tggtctccat
     6421 ccgggcaaag gatcgcagcg ccggcacgta ccactccgtt tcatagcgat acatggtccc
     6481 gtaccagaag gacttctcgc ccagattgag cgagcgtcgc agcaccaggg catgaaagct
     6541 ttgaccggcg acctgtatgt cctcccatcc ttcggcgtaa aggctggcgg tcacgtagtt
     6601 gcggatgccg cccgggtcgt cgcgatccag gtagaacgca tcgcgccatt cctttccagc
     6661 caccagcgga aaatcgtatt cgcgaaacgg cgtttggcag cggccgaaca tcgggccatc
     6721 ctcggtaaag gtgccggcca attgcaggcc aggcgcttcg tagcgggcgt catcgaggat
     6781 gtggccgtgt tcgtcctgcg tgacgatgcg gatgccgttc tcttcgacgg cccggacctc
     6841 gcgggtcagg gtgccgcgcg ggaggccgtt gtatccatcg gtccgtttat agatccaacg
     6901 atcgccaacc cgatacgtcg gtgcggggac ttcggtgagc tcgtaaaccc cccgcggagc
     6961 ggggcgtatc gcgcacccgg cgagcgccag ggcgcccgcg ctgactagaa agcttctgcg
     7021 atccattacg attctccgat gggaggtttg ggttccaatt cagaccctgc gcggcagaga
     7081 aggtgcgaag ccggtcgagc gagcaattgt gccaagcgaa gcgggaaggt aaaatccgcg
     7141 gcagatatgg ggacgtagct cagctgggag agcgtcgcgt tcgcaatgcg aaggtcggga
     7201 gttcgatcct cctcgtctcc accagtcatc cgaagggccg gcggttgccg gcccttcgtc
     7261 catctacggg ccggccagcg actgtcgacc ctgcgcttgc atcccgcatg ccggaggcgc
     7321 catgaatgct tcgcccacgc atgaccgatg gcgacgcctg tggctgctgt gcggctgggt
     7381 gatgatcggc ctcgtcattc tgttctcgct gattcccgcg ccgccgatat tggatgtgtc
     7441 gcaaggcgac aagctgcagc atctcggcgg ttatgccctg ctgatggcgt ggttcgccca
     7501 agtctatcgc cgtcccggtt cacgcatgtg cgtggcgctg ggtctgaccg cgctcggcat
     7561 tacgctcgag gtgctgcagg gcatgaccgg ctggcgcagt ttcgagtcgt ccgacatggt
     7621 ggccaatacg gcgggtgtgc tgctcggttg gctgcttgcg ccgccgcgcg gacccgagct
     7681 gtaccgccgg gtggcagacc gagaccggct ggcggcaaac gctcaatcca gcgcgcggta
     7741 aaagtcttcc acgagaccgt tgaatgcttc ggcctgttcg atattggaca aatgcgctgc
     7801 cgaggcgatc accgccagcc gcgagccttc gatcgcttcg tggatctcgc gtgccatttc
     7861 gatcggcgtg ccgctgtcct gatcgccgac gatcaccaat gtgggacagc gcaggctgcg
     7921 aagcgcttcg gtgaaattca gtttcgcgat cgcctgcccg cagccggcat agccttccac
     7981 cggcgtggcg cggatggtgt caccgatgcg ctgcatcact tccggatggg cgtcgcggaa
     8041 gggtttggtg aaccagcgtt ccaaggtggc gcttaccact gcttccatgc cctggtcgcg
     8101 taccgtccgg atgcgctcgg cccagaccgt ttccacatcg gcggggtaac ggctgctggt
     8161 gtcggccagc acgaggctgg cgacgcgctg gggatacgca agtgcgaacg cctggccgat
     8221 catgccgccc atggaaagcc cgagccaatg cgcacgttcg atgcctagcg cgtcgaacag
     8281 ggccgcagcg tccgccgcca gcatgtcgaa actgtacggc cccggcggcg cgtcgctcgc
     8341 gccgtggccg cgcgtatcgt aacgcagcac ggtgtagcgc tgcgcgagct gcgctgcctg
     8401 cgcgtcccac atgcgatggt cgcaggccaa tgcatggctc atgacaatcc agggaccctg
     8461 cccttcgatc ctgtagtgca ggtcgatgcc gttggcgcgt actttgttca tgccacctcc
     8521 ttcgcgacga acgaggtcat tgtcggccac gcaccgcact gccacaagac gcgggggtta
     8581 ttccttgacg cgcgacagac ggactacgtc atggacgcgg cggaccgctt tcatgacgcg
     8641 tgccagatgc aggcggttgc gcacctggat ggtgaacagc atggtggtgt agaggccgcg
     8701 ctcttcgtcc atgctgacgt tgtcgatgtt cgagcccgct tcggcgatgg ccgcagccac
     8761 cttcgccagc acgccgcgct ggttcgccgc gacgacgcgc acgcacacgt cgaacagctt
     8821 gctcggatcc gcctcccatt cgacgtcgag ccacttgtcc gggtcggtgc gcgacttggc
     8881 cgcgaccggg cagtcggtgg tgtgcaccac cagaccctgg cccttcttga tgaagcccat
     8941 gatcggatcg ccggggatgg gtcggcagca tttgccgaac tgcaccgcga tcccttcgga
     9001 gccgcgaatc acgatcgagc cggccggctt cgtatcggca gtctggtcgg tcagcgccaa
     9061 caattggcgc gcgacgatgg cggccagtcg cttgccgagt ccgatatcgg ccagcacttc
     9121 ttcgcgcgac ttggcgccgc tttcgcgcac cagccgttcc cactgctcgc tggcgatgtc
     9181 ggcgaggtcg gcatccaggc tgcgcagcgt ctgattgagc agtcgctcgc ccagcgacac
     9241 cgattcctcg aactgcacgg tcttgaggaa atggcggatc tgcgagcgcg ccttgccggt
     9301 cttcacatag gacagccagg ccggattcgg gtgcgcgtgc gcagcggtga tgatttccac
     9361 gcggtcgccg ttgcgcagtt ccgtacgcag cggcgccagt tcgtaattga ccttcgcggc
     9421 gacgcagcgg ttgccgatgt cggtgtgcac ggcataggcg aagtccacgg gtgtcgcgcc
     9481 gcgcggcagc gacatgatct tgcctgcggg cgtgaagaca tagacttcgt cgggaaagag
     9541 gtcgaccttg acgtgttcga ggaactcggc cgagtcgcca ctggcgctct ggatttccac
     9601 cagcgattgc agccagttgt gggtgcggcg ctgcacctcc gacgtcgccg cttcctcttc
     9661 cttgtacagc cagtgcgagg ccacgccggt ttccgcgata tggtgcattt cgcggctgcg
     9721 gatctgcact tccacgggcg taccgaacgg cccgatcaac gcggtatgca gggactgata
     9781 gccattcgcc ttcgggatgg cgatgtaatc cttgaacttg ccgggcaccg gcttgtacag
     9841 cgaatgcagc gcgccgagcg cgagatagca cgacggcaca tccttcacca ccacgcgaaa
     9901 gccgtagatg tcgagcacct cggaaaagga cagcgacttc tccagcatct tcttgtagat
     9961 ggagtagatc cccttttcgc ggccggtgat cgatgcctcg atgccttcgt cggccagctt
    10021 cttggtgatc gcctccagaa tcttgttgac gacttcacgg cggtttccgc gcgcggcctt
    10081 caccgccttc gcgatggtgc ggtagcgcag cggatagagg tgcttgaagg acagttcctg
    10141 cagttcgcga taaagcgaat ccaggccgag acggctggcg atgggcgcgt agatttcgat
    10201 ggtctcgcgc gcgatacgat ggcgcttgtg catgcccacc gcgtcgaggg tgcgcatgtt
    10261 gtgcaggcgg tcggccagtt tgatcaggat gacgcgtacg tcgcgcgcca tcgcgagcat
    10321 cattttgcgg aagttctccg cctgcgcgtc cttctggctc tggaattcaa tcttgtcgag
    10381 cttggatacg ccgtcaacga gttccgctac ggcgggtccg aaacgttcgc tgatatcgtc
    10441 cttcgagacc ttggtgtcct cgacgacgtc gtgcagcagc gccgccatga gggtctgcgg
    10501 gtcgagctgc cattccgaaa ggatttcagc gaccgcgatg gggtgggaga tgtacggttc
    10561 gccgcttttg cgaaactgcc cctgatgcgc agcgtcgctg aagcggtagg cttcctcgat
    10621 gcgggagaga tccgccggct tgaggtagcc ggcgagattg agcttcagtt cgttcagtgc
    10681 cggaacggcg ctcatgtccg cccc
//
LOCUS       DATGOU010000088        12729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_2454462, whole genome shotgun sequence.
ACCESSION   DATGOU010000088 DATGOU010000000
VERSION     DATGOU010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12729
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2454462"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VKZ48_05350"
     CDS             <1..546
                     /locus_tag="VKZ48_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HLU77313.1"
                     /translation="PPAPRGMPQIEVTFDIDANGILHVSAKDKATGKENKITIKANSG
                     LSEDEIQRMVKDAEAHAAEDKKTLELVTARNQADALVHSVKKSLTEHGDKIDADEKSK
                     IEAAVKDLEEALKGDDKDAIDSKSAALAQASQTLGEKIYADAQAAAGAAGAGGSGAGE
                     SAQQADDNVVDAEFTEVKDKK"
     gene            642..1763
                     /gene="dnaJ"
                     /locus_tag="VKZ48_05355"
     CDS             642..1763
                     /gene="dnaJ"
                     /locus_tag="VKZ48_05355"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HLU77314.1"
                     /translation="MTKRDYYEVLGVNRDAGEDELKRAYRKLAMKYHPDRNPDNPKAE
                     EHFKEAKEAYEILSDQNKRAAYDRYGHAGVDPSAGAGAGAGYANFADAFGDIFGDIFG
                     GGRAGGRSSVYRGADLRYNLEITLEQAAHGTETKIRIPTAEECDTCHGSGAKPGTQPQ
                     ACPTCDGHGQVRMQQGFFSIQQTCPRCHGTGTYIADPCTGCSGAGRVKTHKTLSVKIP
                     AGVDEGDRIRLAGEGEAGVNGGPSGDLYVQIHLKAHPVFQRDRDDLHCEMPISFPTAA
                     LGGEVEIPTLDGVAKIKVPAETQSGKTFRLRGKGIKGVRSSTYGDLYCHVVIETPVNL
                     TERQKALLREFDAITQDNSERHNPRAKTWMNRVKEFFSV"
     gene            1975..3102
                     /locus_tag="VKZ48_05360"
     CDS             1975..3102
                     /locus_tag="VKZ48_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLU77315.1"
                     /translation="MLRKSIAQWATVAGLALVGAATVQAAEPIIIGQSAPLTGSNKEL
                     GEDIRDGALAYFHKVNADGGINGRKIELITLDDQNKTDLSKQNTAELLDKHNAVALFG
                     YASTTLSKPAMPHVEKAKVPFVAPFTGADWMRTFRPLVFNHRAGYGDELEKVIEHYTT
                     FGLKRFAVLHHDDTVGSENLAAVERALKARDLKAVSIASIKRTQKDIAQEVATVVKSN
                     PEVVICTTLYRTTADFVKQSRKLGLNAQVVSLSFAGSTALAGALGEAGLGVVMSQVVP
                     TPTKASVPIVREYHDAIKAIAPDKVHSFTGLESYIAAKILVEGVRRAGPNVTRASLLK
                     GLESINNYDLGGYVVSFSPKDHNGSKYVGLTILGRDLAFKD"
     gene            complement(3205..4575)
                     /gene="cysS"
                     /locus_tag="VKZ48_05365"
     CDS             complement(3205..4575)
                     /gene="cysS"
                     /locus_tag="VKZ48_05365"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HLU77316.1"
                     /translation="MLKIYNTLTRSKQAFVPIEPGRVRMYVCGMTVYDYCHLGHARVM
                     VVFDMVQRWLRASGYDVTYVRNITDIDDKIIRRAAENGESIAALTDRFIAAMHEDADR
                     LGVQRPDYEPRATEYVDAMLDMIGTLEQKGYAYRAQSGDVNYAVRKFSGYGKLSGKSL
                     DELRAGERVEVDRDKHDPLDFVLWKHARADEPAWPSTYGDGRPGWHIECSAMSSQLLG
                     KHFDIHGGGEDLQFPHHENEIAQSEAAHDCSFVNYWMHNGFVRVDDEKMSKSLGNFHT
                     IRDVLQRYDAEVVRFFILRAHYRSPLNYSDQHLDDARHALTRLYTALKGVPPADNVQI
                     DWSARYPAAFREAMNDDFNTPEAMAVLFELANEVNRSGSPQAGLQLRALGGVLGLLQR
                     DANDFLRGDLPEGWTEARIEEQIAARAAAKKAKNYAEADRIRQELGEAGIVLEDSPAG
                     TTWRQA"
     gene            4772..5287
                     /locus_tag="VKZ48_05370"
     CDS             4772..5287
                     /locus_tag="VKZ48_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLU77317.1"
                     /translation="MRILVAVFALLYACALHAAPIDDAREMLRAGEAERALRFLDTHL
                     KANPRDTEARFLKGVVLTDQGNTAAAIEVFRDLTREYPELPEPYNNLAVLYAAQGDYR
                     AARDALDTAVRVKPDYAKAYENLGDVHVLLARDAYNKALSLDGANGRVRAKLNLAREL
                     SEYAPRPASVP"
     gene            5325..5870
                     /locus_tag="VKZ48_05375"
     CDS             5325..5870
                     /locus_tag="VKZ48_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLU77318.1"
                     /translation="MLSLVASAALAAGPQVALETNQGRIVLELDADKAPRTVENFVDY
                     VQSGHYDGTIFHRVIDGFMIQGGGFTADMRQKPTRAPIENEAANGLRNDVGTIAMART
                     RDPHSATAQFFINVNDNTSLNHSAQTVAGYGYAVFGRVVQGMEVVQRIAKLPTTRVGM
                     HADVPREPVVIEKATLLPTQR"
     gene            5880..6371
                     /locus_tag="VKZ48_05380"
     CDS             5880..6371
                     /locus_tag="VKZ48_05380"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016345694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLU77319.1"
                     /translation="MVKLHTNFGTITLELDAERAPETVKNFLAYAESGFYNNTVFHRV
                     IDGFMIQGGGFEPGMRQKPTNAPVKNEADNGLKNDKYTIAMARTSDPHSATAQFFINV
                     KDNDFLNFSAPTPQGWGYCVFGRVVEGAEVVDKIKAVPTGTSGFHQDVPREDVIIERA
                     EVV"
     gene            6469..7194
                     /locus_tag="VKZ48_05385"
     CDS             6469..7194
                     /locus_tag="VKZ48_05385"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF003743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HLU77320.1"
                     /translation="MKPYLFVADLHLAAERPALYETFLRFLDERVRHAQALYILGDLF
                     EYWVGDEDADAPFHRGVMDALADVAEMDVPVYLMHGNRDFLVGKRAAARGNLRLIADP
                     TVIDVHGTPTLLMHGDTLCTDDIAYQRIRARLRRPLILRTFLALPFGVRRRIGAALRA
                     KSERTKRHTPPEIMDVNRQAVENVLRSYGYPRLIHGHTHRPARHMHHVDGTVCERWVL
                     GDWYKRGSYLECDAAGCRAVVLD"
     gene            complement(7278..8453)
                     /locus_tag="VKZ48_05390"
     CDS             complement(7278..8453)
                     /locus_tag="VKZ48_05390"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HLU77321.1"
                     /translation="MTEEFDFDTRAIRAGTVRSQFNEHSEALFLTSSFRFKNAAQAAA
                     RFAHTEEGPVYSRFSNPTVNMFTDRLASLEGAEACVATSSGMSAILATVMGLLKGGDH
                     VICSAGVFGATVQLFGNVLSKFGLETTFVSGTEASAFAAAVKPNTRMLFIETPSNPLL
                     EVFDIAALKQVAHKAGALLVVDNCLCTPALQRPLELGADLVVHSATKYIDGQGRVLGG
                     AILGSKALVTDVIGPALRTTGPSLSPFNAWVLLKGLDTLRLRMDAHAANALAIAQWLE
                     KHPRVARVNYPGLESHPHYQLARKQQRNGGAMLSFEVKGGREAAWRVIDSTRMISITA
                     NLGDTRTTITHPATTTHGRISAEAREAAGISEGLIRVPVGLESLEDIKADLKRGLDA"
     gene            complement(8771..10210)
                     /gene="purF"
                     /locus_tag="VKZ48_05395"
                     /pseudo
     CDS             complement(8771..10210)
                     /gene="purF"
                     /locus_tag="VKZ48_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004544727.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
     gene            complement(10234..10725)
                     /locus_tag="VKZ48_05400"
     CDS             complement(10234..10725)
                     /locus_tag="VKZ48_05400"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HLU77322.1"
                     /translation="MTWVDWAVIAVLGISAMLGLWRGFIREVLALVGWVVAGVIAVLF
                     AADIAAAMPADFTTPLVRHLLAAALIFVLVLVVAALAASLLSKLVRAIGLGPLDRTLG
                     GLFGFVRGGVIVLIAVLLAGMTPLPRDPAWQGAQLKGPLETAAIMARAYLPEEIAARI
                     RYR"
     gene            complement(10722..11312)
                     /locus_tag="VKZ48_05405"
     CDS             complement(10722..11312)
                     /locus_tag="VKZ48_05405"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HLU77323.1"
                     /translation="MAHADEALDLKRRARRRLIGAIALVLFLVIVPPWIMDLEPQPVE
                     TTLEVEIPQPDERPLPVVPPQTEGPAPEQPAAPAPAVEAPAAAPQAETPKQTAKPATP
                     PAGSKPEAKADKPAAAKGEAYVVPVATLSTEGNVKELQAKLSLAGLESYTEPVKTASG
                     QYTRVRAGPYASKAEAEKVHARLREMGLKPGNVTTR"
     gene            complement(11330..12613)
                     /gene="folC"
                     /locus_tag="VKZ48_05410"
     CDS             complement(11330..12613)
                     /gene="folC"
                     /locus_tag="VKZ48_05410"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016355366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HLU77324.1"
                     /translation="MSLPTDLAGWLDYIERLHPATIDLGLERVQRVQAVLDIPKFCPL
                     FIVGGTNGKGSTCTMLEAILHAGGYRVGKYLSPHLVRYNERVQIDGAEAGDADLIAGF
                     EAVEAARGDVQLTYFEYTTLAAWVTFSRAGLDALVLEVGLGGRLDAVNVFDPDCSIIT
                     SVDVDHVDYLGDSREQIGWEKAHIYRPGRPAICSDPQPPQRLVEHAQAIGADLRLIGR
                     DFGYEGDRSQWLYWGEKGRRSGLPYPALRGANQLLNAAACLAALEAMHERLPVSAQHI
                     RTGLLAAQLPGRFQVLPGRPTRIFDVAHNPHAAAVLRENLIAMPGYRRTIAVVGMLKD
                     KDIGGVFAHLKDRVDVWYVATLDNPRGATAAELEEAIRQAGAEGPIEQCATPHEAYAR
                     AQEAATESDRILVFGSFYTVADVMAMPESPAPAAR"
     gene            complement(12625..>12729)
                     /locus_tag="VKZ48_05415"
     CDS             complement(12625..>12729)
                     /locus_tag="VKZ48_05415"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006998260.1"
                     /note="catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA; forms a tetramer of AccA2D2 subunits; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit beta"
                     /protein_id="HLU77325.1"
                     /translation="LEKGAIDLIVDRRQMRERLASLITLLMRIPAPTV"
BASE COUNT         2357 a         4169 c         4022 g         2181 t
ORIGIN      
        1 ccgccggcac cgcgcggcat gccgcagatc gaggtgacct tcgacatcga cgcgaacggc
       61 atcctacacg tctcggcgaa ggacaaggcg accggcaagg aaaacaagat caccatcaag
      121 gccaactccg gactgtcgga agatgaaatc cagcgtatgg tgaaggacgc cgaagcccac
      181 gccgcggaag acaagaagac gctggagctg gtgacggcgc gtaaccaggc cgatgcgtta
      241 gtccattcgg tgaagaaatc gctcaccgaa cacggcgaca agatcgacgc cgacgagaag
      301 tcgaaaatcg aggcggcggt caaggacctt gaagaagccc tcaagggcga cgacaaggac
      361 gccatcgaca gcaagtccgc cgccctggcg caggcctcgc agacgctcgg cgagaagatc
      421 tacgccgatg cgcaggctgc ggccggtgcc gcaggagccg gcggcagtgg cgccggcgaa
      481 agcgcgcaac aggcggacga caacgtggtg gatgccgaat tcaccgaagt gaaagacaag
      541 aagtaagcaa gcggagagca gcgagcggag agcggagagc tgaaacctct tcgctctccg
      601 ttttcgctcg tggctctcgg ctctttgctc acagccagaa aatgaccaaa cgcgactatt
      661 acgaagtgct cggcgtcaat cgcgatgccg gggaagacga gctgaagagg gcctaccgca
      721 agctcgcgat gaaataccac ccggaccgca atccggacaa ccccaaggcg gaagagcact
      781 tcaaggaagc gaaggaagcc tacgagattc tctcggacca gaacaagcgc gccgcctacg
      841 accgttacgg ccacgcgggt gtcgatccga gcgcgggcgc cggcgcgggc gcgggctacg
      901 ccaacttcgc ggatgccttc ggcgacatct tcggcgacat cttcggcggc gggcgcgcag
      961 gcggccgctc gtccgtttat cgcggcgcag acttgcgcta caacctggaa atcacgctcg
     1021 aacaggcggc gcatgggacg gagaccaaaa tccgcattcc gaccgccgaa gagtgcgaca
     1081 cctgccatgg cagcggtgcc aagcccggca cgcaacccca ggcctgcccg acctgcgacg
     1141 gacacggtca ggtccggatg cagcagggct tcttttcgat ccagcagacg tgtccgcgct
     1201 gtcacggcac gggcacgtac atcgccgacc cgtgtaccgg ttgcagcggc gcggggcgcg
     1261 tcaagaccca taagacgctg tcggtgaaga tccccgccgg cgtggacgaa ggcgaccgca
     1321 tccgcctcgc gggcgaaggc gaagccggcg tgaacggcgg cccctccggc gacctttatg
     1381 tgcagattca tctcaaggcg caccccgtct tccagcgcga ccgcgacgat ctgcattgcg
     1441 aaatgccaat cagcttcccc accgccgcgc tcggcggcga agtggagatc cccacgctgg
     1501 acggcgtggc gaaaatcaag gtgcccgcgg aaacgcaaag cggcaaaacc ttcaggctgc
     1561 gcggcaaggg catcaaaggc gtgcgctcca gcacctatgg cgacctgtac tgccatgtgg
     1621 tgatcgaaac gccggtcaat ctcaccgaac gccagaaggc cctgctgcgc gagttcgacg
     1681 ccatcacgca ggacaacagc gagcgccaca acccgcgtgc caagacctgg atgaaccggg
     1741 tcaaagagtt tttcagcgtc tgacggcacg cggcaaccgc ggctgtcgcg cccctctccc
     1801 gcagcgtcgc cctctccctg cctgcccggg cattcgcggc atttgtgaat tattaccggt
     1861 gcaagccggg cggcttgggt cgaatgggaa atgtgtccta gtctgccccg cgagcttgac
     1921 ggacgatcac gcaagatcaa gaaagccgtc acgctacacg ggagaggaga gcacatgctt
     1981 cgcaaatcga ttgcgcaatg ggcgaccgtt gcagggctcg cgctggtcgg cgcggcgacc
     2041 gtgcaggcgg ccgaaccgat catcatcggg cagtctgccc cgttgaccgg ctccaacaag
     2101 gaactgggcg aggacatccg cgacggcgca ctggcctact tccacaaagt caacgcggac
     2161 ggcggcatca atggccgcaa gatcgaactg atcacgctcg acgaccagaa caagaccgac
     2221 ctgtccaagc agaacactgc ggagctcctg gacaagcaca acgccgtggc gctgttcggt
     2281 tacgccagca cgacgctcag caaaccggcg atgccccatg tcgagaaggc gaaggtcccc
     2341 ttcgtcgcgc cctttaccgg cgccgactgg atgcgcacct tccgcccgct ggtgttcaac
     2401 caccgcgccg gctatggcga cgaactggaa aaggtgatcg agcactacac caccttcggt
     2461 ctgaagcgct tcgccgtgct gcaccatgac gacaccgtcg gctcggagaa cctcgccgcc
     2521 gtggaacgcg ccctcaaggc gcgcgatctc aaggccgtgt cgatcgcgtc catcaagcgg
     2581 acccagaagg acatcgccca ggaagtcgcc acagtggtga aatcgaatcc cgaggtggtg
     2641 atctgcacga cgctctatcg gaccaccgcg gacttcgtga agcagtcgcg caagctggga
     2701 ctcaacgccc aggtcgtcag cctttcgttc gccggctcca ccgcactggc cggggccctg
     2761 ggcgaagcgg gcctcggtgt ggtgatgtcg caggtcgtgc ccacgccgac caaggcctcg
     2821 gtgcccatcg tgcgcgagta tcacgacgcg atcaaagcga tcgcccccga caaggtgcac
     2881 tccttcaccg gcctggagag ctacatcgcc gccaagatcc tggtcgaggg cgtccgccgc
     2941 gccggcccca acgtgacccg tgccagcctc ctcaagggcc tggagtcgat caacaactac
     3001 gatctgggtg gctatgtcgt cagcttctcg cccaaggatc acaacggcag caagtacgtc
     3061 ggcctgacga tcctcggccg cgacctagcc ttcaaggact aacaagaagc agaagcacga
     3121 agaagaacaa gccttcgcgc aaaaacgcgc actcaatgaa acgggcggcc ttggccgccc
     3181 gttctttttg gtgtgagaga acagctaggc ctgccgccag gtcgtcccgg ccggcgaatc
     3241 ctccagcacg atgcccgcct cacccagttc ctggcggatg cggtcggcct cggcatagtt
     3301 cttcgctttc ttggccgccg cacgcgccgc gatctgctct tcgatccgcg cttccgtcca
     3361 gccttccggc aagtcaccgc gcaggaagtc gtttgcatcg cgttggagaa ggcccagcac
     3421 accgcccaaa gcgcgcagtt ggagccccgc ctggggcgag ccgctgcgat tcacttcatt
     3481 ggccagttcg aacagcacag ccatcgcttc cggcgtgttg aagtcgtcgt tcatcgcctc
     3541 gcggaacgcg gccgggtagc gtgcgctcca gtcgatctgc acgttatcgg cggggggtac
     3601 gcccttgagc gccgtgtaca ggcgggtgag cgcgtgccgc gcatcgtcca gatgctggtc
     3661 ggaatagttc agcggactgc ggtaatgcgc acgcaggatg aagaagcgca cgacttccgc
     3721 gtcgtaacgc tgcagcacgt cgcggatggt gtggaagttg ccgagcgatt tcgacatctt
     3781 ctcgtcgtcc acgcgcacga agccattgtg catccagtag ttgacgaagc tgcaatcgtg
     3841 cgcggcctcc gactgggcga tttcgttttc gtgatgagga aactgcagat cctcgccgcc
     3901 gccgtggata tcgaaatgct tgcccagcaa ctggctgctc atggccgaac attcgatatg
     3961 ccagcccggc cgcccgtcgc cataagtcga aggccaggcc ggctcgtcgg cgcgggcgtg
     4021 cttccacagc acgaaatcga gcggatcgtg cttgtcgcga tcgacttcca cgcgttcgcc
     4081 cgcacgcaac tcgtcgagcg atttgcccga gagcttgccg tagccggaga acttgcgcac
     4141 cgcatagttg acgtcgccgc tttgggcgcg ataagcatag cccttctgct ccagcgtgcc
     4201 gatcatgtcg agcattgcgt cgacatactc ggtcgcgcgc ggctcgtagt cgggccgctg
     4261 cacgcccaga cggtccgcgt cctcgtgcat tgcagcgatg aagcggtcgg tcagcgcagc
     4321 gatcgattcg ccgttttctg ccgcacggcg gatgatcttg tcgtcgatat cggtgatgtt
     4381 gcgcacatag gtgacgtcgt agccgctggc gcgcagccac cgctgcacca tatcgaacac
     4441 gaccatcaca cgggcgtggc ccaaatggca gtaatcgtag accgtcatcc cgcagacata
     4501 catccgcacg cgtcccggtt cgatgggcac gaatgcttgc ttgctgcggg tcagagtgtt
     4561 gtagatcttc agcatgaaaa ggaaaacgcc ggagcgtgcc ggaagcagga ctgttagaat
     4621 cgcgcgggtt cgccggcacg gcgctccgcc acacggcgcc gggcgccgca gtatagccga
     4681 gtatggcgca gcgattaagg ctcgccgggt gccgcaggag gcgccgcacc cggccgacga
     4741 cgctgcccgg cattcaccgt tggatgtttc gatgcgcatc ttagttgccg tcttcgccct
     4801 gctttatgca tgcgccctgc atgccgcgcc catcgacgac gcgcgcgaaa tgctgcgcgc
     4861 cggcgaagcg gagcgtgcgc tgcgcttcct cgatacccat ctcaaagcga atccccgcga
     4921 caccgaggcg cgcttcctca agggcgtcgt gctgaccgac cagggcaaca cggccgcggc
     4981 catcgaagtg ttccgcgacc tcacgcgcga atacccggaa ctgccggaac cgtacaacaa
     5041 tctggctgtg ctatacgccg cgcagggcga ttaccgtgcg gcgcgcgacg cgctcgacac
     5101 ggcggtgcgc gtgaagcccg attacgccaa ggcctacgag aatctgggcg acgtccacgt
     5161 gctgctcgcg cgcgacgcct acaacaaggc cctctcgctc gacggcgcca acgggcgcgt
     5221 ccgggcgaaa ctgaatctgg cgcgcgaact gtccgagtac gcgccccgcc ccgcttccgt
     5281 tccgtaatca tggagtgcct gtgctgaaat ctttgttgtc gttgatgctg tcgttggtcg
     5341 caagcgccgc gctcgcggcc ggtccgcaag tcgcgctgga gaccaaccag ggccgtatcg
     5401 tgctcgaact ggacgcggac aaggcgccgc gcacggtgga gaacttcgtc gactatgtgc
     5461 aatccggtca ttacgacggc acgattttcc atcgtgtcat cgacggcttc atgatccagg
     5521 gcggcggctt caccgccgat atgcgccaga agccgacccg cgcccccatc gagaacgaag
     5581 ccgccaacgg actgcgcaac gacgtgggta ccatcgccat ggcacggacg cgcgacccgc
     5641 actcggccac cgcacagttc ttcatcaatg tgaacgacaa taccagcctg aatcacagcg
     5701 cgcagacggt ggcaggctat ggttatgccg tgttcggccg cgtggtgcag ggcatggagg
     5761 tcgtccagcg catcgccaag cttcccacca cgcgcgtcgg catgcatgcc gacgtcccgc
     5821 gtgaaccggt ggtcatcgaa aaggccacgc tccttcccac ccagcgataa ggattcctca
     5881 tggtcaagct gcataccaac ttcggcacca tcaccctcga actggacgcc gagcgcgcgc
     5941 cggagacggt caagaacttc ctcgcctacg ccgaaagcgg cttctacaac aacaccgtat
     6001 ttcatcgcgt gatcgacggc ttcatgatcc agggcggcgg tttcgagccc ggcatgcgcc
     6061 agaagcccac caacgcgccg gtgaagaacg aggcggacaa cggcctcaag aacgacaagt
     6121 acaccatcgc catggcgcgc acctcggacc cgcattcggc caccgctcag ttcttcatca
     6181 acgtgaagga caacgacttc ctcaacttca gcgcacccac gccgcagggt tggggctact
     6241 gcgtattcgg ccgtgtcgtg gagggcgccg aggtggtgga caagatcaag gcggtgccga
     6301 ccggcacgag cgggttccac caggatgtgc cgcgcgagga cgtcatcatc gagcgcgcgg
     6361 aagtcgttta atgtccagca gagggccgag agtcgagagc cgagagtatg cctcccgcag
     6421 tcggccttta ctctccactc tctactcgcc actctgcact gtttcggttt gaagccctat
     6481 ctcttcgtcg cggacctgca tctcgccgcc gagcgaccgg cgctgtacga gaccttcctg
     6541 cgctttctgg atgagcgcgt gcgccatgcg caggcgctgt acatcctcgg cgacctgttc
     6601 gagtactggg tcggcgacga ggacgcggac gcccctttcc accggggcgt gatggacgcc
     6661 ctcgccgatg tggccgaaat ggatgtgccc gtctatctca tgcacggcaa ccgcgatttt
     6721 ctggtcggca agcgcgctgc cgcgcggggc aatctgcgcc tgatcgccga ccccacggtg
     6781 atcgacgtgc acggcacgcc gacattgctg atgcacggcg atacgctatg caccgacgat
     6841 atcgcctatc agcgcatccg tgcgcggctg cggcggccgc tgatcctgcg caccttcctg
     6901 gccctgccct tcggcgtgcg ccggcgcatc ggcgcggcgt tgcgtgccaa gagcgaacgc
     6961 accaagcgcc ataccccgcc cgaaatcatg gacgtgaaca ggcaggcggt ggaaaacgtg
     7021 ttgcgcagtt acggctatcc gcgcctcatc cacggtcata cgcaccggcc cgcccgccat
     7081 atgcatcacg tggacggcac cgtatgcgaa cgctgggtgc tgggcgactg gtacaagcgc
     7141 ggcagctatc tggaatgcga cgccgccggc tgtcgcgccg tggttctcga ttagcaagat
     7201 cctgcaccga gggcggcccg atccgggtcg actcgattcg gccgcgcgcg cgcgcggtat
     7261 ctccttaacg tcgccgctca ggcgtccagg ccgcgcttga ggtcggcctt gatatcttcg
     7321 agcgactcca gtccgaccgg cacgcggatg agtccctcgc tgatgcccgc cgcctcgcgc
     7381 gcttcggccg aaatgcgacc gtgcgtggtg gtggccggat gggtgatggt ggtgcgggta
     7441 tcgcccaggt tggcggtgat ggaaatcatc cgggtgctgt cgatgacgcg ccaggctgct
     7501 tcgcgcccgc ctttcacttc gaacgacagc atggcgccgc cgttacgctg ttgcttgcgt
     7561 gccagttggt agtgcgggtg cgattccagg ccgggatagt tgacgcgcgc gacgcgcgga
     7621 tgcttttcca gccattgggc gattgccagc gcgttggccg catgggcatc catgcgcagg
     7681 cgcagcgtat ccaggccctt gaggagcacc caggcattga agggagagag actcgggccg
     7741 gttgtgcgca gcgccgggcc gatgacgtcg gtcaccagcg ccttggagcc gaggattgcg
     7801 ccgcccagca cccgcccctg cccgtcgatg tatttggtcg cggagtgcac cacgaggtct
     7861 gcgcccaact ccagcgggcg ctgcagcgcg ggcgtgcaca ggcagttgtc gacgaccagc
     7921 agcgcgccgg ctttgtgcgc gacctgcttc agggcggcga tatcgaacac ctccagcagc
     7981 gggttggagg gcgtttcgat gaaaagcatg cgggtattgg gcttgacggc cgcggcaaac
     8041 gcgcttgcct ccgtgccgga cacgaaggtg gtttcaaggc cgaacttcga cagcacgttg
     8101 ccgaacaact gcaccgtcgc gccgaacacg ccggccgagc agatgacgtg atcgccgccc
     8161 ttcaacaggc ccatcaccgt tgcgaggatg gccgacatgc cggaggacgt cgcgacgcag
     8221 gcttccgcgc cttcgagcga ggcgagccgg tcggtaaaca tgttcaccgt aggattgctg
     8281 aagcgcgaat agaccggccc ctcttccgta tgcgcaaagc gcgccgctgc ctgtgccgcg
     8341 ttcttgaacc ggaaactgga agtgaggaac agcgcctcgg aatgctcatt gaactgactg
     8401 cgcacggtgc cggcgcggat cgcgcgtgta tcgaaatcga attcttccgt cattttcatc
     8461 cccagcgtga tgccggaccg gcggcgcgac gcccgtcgcg cccgttccgg tcggcccaaa
     8521 aaacaaaacc cgccctgcct gcggctagaa cgggttcgtg tgcgggccat cccgcgcgat
     8581 tcccacgctt tagccgtatt tgtttgtcgc ccgccggtta tccggtgcgg cggcgcccgc
     8641 aagctgtgct caaatcggcg ctgtcgcgga ccttaatgcc cacggtcatg gcttgtcaaa
     8701 cggtggcgtt ctcgtccgac tcgtcttcgt tctcttcgtc gggttgatcg cgctcgccgt
     8761 cgcggcggtg ttcgacgtcg ttcagatact cgacggtgac gtcgccggtt acgtaacggc
     8821 cgtcgaagca ggaagtctcg aattccgtca gtgccgggtt cacttcacgc accgaggctt
     8881 cgagcgcctc caaatcctgg tagatcacca catcggcttc gatttcgcgc gcgatctcct
     8941 cttcggtgcg accggtggcg atcagttcct gccgcgtcgg catgtcgatg ccgtacacgt
     9001 tgggaaagcg caccggcggc gcggccgagg cgaaataaac tttgcgcgca cccgcttcgc
     9061 gcgccatctg cacgatctcg cggctggtgg tgccgcgcac gatggagtcg tcgacgatca
     9121 gcacgttctt gccctggaac tccataccga tggcgttgag cttctgccgc accgaacgtt
     9181 tgcgcacgtt ctggccgggc atgatgaagg tgcggccgat atagcggttc ttgacgaacc
     9241 cttcgcggta cgagatgccc aggcccagcg ccagttgcat ggcgatgggg cggctggtgt
     9301 ccggcacagg aatgaccacg tcgatatcca gatccgggta ctgcttgcgg atcttcttcg
     9361 ccagatgctc gcccatgcgc aggcgcgctt cgtacactga cacgccgtcg atgagggaat
     9421 cggggcgggc aaggtagacg tgttcgaaaa tgcagggcgt caatcgcgcg tcgtccacgc
     9481 actgctgact gtggaactcc ccgctctcgg taatgacaat gcactcgccc ggcgcgacat
     9541 cgcgcacgaa ctcgaagccc atggcgtcca gcgccacgct ttcggaagcg accatccatt
     9601 ctggaccgtc ttcggtctcg ttgcgtccga tcaccagggg acggataccg tgcggatcgc
     9661 ggaaggccac caggccgagg ccggcgatca tgctgaccac cgcataggcg cctttgcagc
     9721 gccgatggac cttggcgacg gtcttgaaga ccgtctccgg atcgagctga aaaccggtcg
     9781 ccgtcagttc caattcgtgc gcgaagacat tgagcagcac ttccgagtcg gaactggtat
     9841 tgatgtgacg ccggtcgagc tgaaacatct cccgcttcag ttgctcggca tttgtgaggt
     9901 tgccgttgtg gccgagcacg atgccgaaag gcgagttgac gtagaagggc tgcgcctcat
     9961 cggcattcct ggcagagccg gccgtggggt agcggcagtg gccgatgccg atattgccgg
    10021 gcagcgaacg catgttgcgc gtgcggaaca cgtcgcgcac cagaccgggc gccttgtgca
    10081 tatggaacat cttgccttcg gaggtcacga tgcccgccgc gtcctggccg cgatgctgca
    10141 gcaccagcaa gccgtcgtac agcaactggt tgaccgggct cttgcctacg acccctacga
    10201 ttccacacat ttgtcagcta ctccgtcgtt gccctagcga tagcggattc gcgccgcaat
    10261 ctcttcgggc agataggctc gcgccatgat tgccgccgtc tccaaaggtc ccttcaactg
    10321 cgcgccctgc catgcgggat cgcggggcag cggcgtcatg ccggccagca acacggcgat
    10381 cagcacgatc acgcctccgc gcacaaaacc gaacaggcca cccagcgtgc gatccagcgg
    10441 acccaaaccg atcgcccgca ccagtttgga cagcagcgag gcggcaagtg ctgcaacgac
    10501 cagcaccagc acgaaaatca gcgcggctgc gagcagatgc cgcaccagcg gcgtggtgaa
    10561 atccgccggc atcgccgcgg cgatgtcggc cgcaaacagc accgcgatta cgcctgccac
    10621 gacccagccg accagcgcca gcacctcgcg gatgaagccg cgccacaggc ccagcatcgc
    10681 cgatatgccg agtaccgcga tgacggccca gtcgacccag gtcatcgggt cgtgacgttg
    10741 cccggtttca ggcccatttc acgcaaacgc gcgtgcactt tttccgcctc ggccttgctg
    10801 gcgtaggggc cggctcgcac acgcgtgtat tgcccggaag cggtcttgac cggttccgta
    10861 tagctttcca gaccggccag actcaacttg gcctgcagtt ccttgacgtt gccttcggtg
    10921 gagagggtag cgacgggcac gacataggcc tcgcctttcg cggcggccgg cttatcggct
    10981 ttggcctcgg gcttggatcc ggcgggcggc gtggccggct tggccgtctg cttgggcgtt
    11041 tcggcttggg gtgccgctgc cggagcctca accgccggcg ccggcgcggc cggctgctcg
    11101 ggtgcggggc cctccgtctg cggcggcacg acaggcagcg gccgctcgtc gggctgggga
    11161 atttcgactt cgagcgtggt ctcgaccggc tgcggctcga gatccatgat ccacggcggc
    11221 acgatgacga gaaacagcac caacgcaatg gcgccgatca gcctgcgccg tgcacgtcgt
    11281 ttcaaatcca gcgcctcgtc tgcatgcgcc atagggtttc ctgtccggtt cagcgcgccg
    11341 ccggtgcagg cgactcgggc atcgccatga catcggccac ggtgtagaac gatccgaaga
    11401 ccaaaattct atcactctcc gtggcggcct cctgcgcgcg cgcataggcc tcatgcggcg
    11461 tggcgcattg ttcgatcgga ccttccgcgc ccgcttgtcg gatggcctcc tccagttcgg
    11521 cagcggtcgc gccgcgtgga ttgtcgaggg tcgcgacgta ccagacatcg acgcggtcct
    11581 tgaggtgcgc gaatacgccg ccgatgtcct tgtccttcag catgccgacg acggcaatgg
    11641 tgcggcggta accgggcatg gcgatgaggt tttcgcgcag caccgccgcg gcatgggggt
    11701 tgtgcgcgac gtcgaatatc cgcgtcggcc gtccgggcag cacctggaag cggccgggca
    11761 gttgtgccgc gagcaggccg gtgcggatgt gctgcgcgct gacgggcagg cgctcgtgca
    11821 tggcctctag cgcggccaga caggccgccg cgttcaacag ttgattcgca ccgcgcagcg
    11881 caggataagg caggccgctg cggcgccctt tctcgcccca gtaaagccac tggctgcgat
    11941 cgccttcata gccgaaatcg cgaccgatca gacgcaggtc ggcgccgatc gcctgtgcgt
    12001 gctcgaccag ccgttgcggc ggctgtggat cgctgcagat cgccggccgg ccgggacggt
    12061 agatatgcgc cttctcccac ccgatttgct cgcgcgagtc gcccagatag tcgacgtgat
    12121 ccacatcgac gctggtgatg atcgagcagt ccggatcgaa gacattgacc gcatccagcc
    12181 gcccgcccag tcccacttcg aggacgagcg catcgaggcc cgcacgcgag aacgtcaccc
    12241 aggccgcaag cgtcgtgtat tcgaagtagg tcagttgcac atcgccccgc gccgcctcca
    12301 cggcttcgaa gccggcgatc aggtccgcgt cgcctgcttc cgcgccgtcg atctgtacgc
    12361 gctcgttgta gcggaccaga tgcggcgaga ggtatttgcc gacacggtaa ccgcccgcat
    12421 gcaggatcgc ctcgagcatg gtgcaggtcg agcccttgcc gttggtaccg ccgacgatga
    12481 acagcggaca gaatttcggg atgtcgagca cagcctggac acgctgcacg cgttccaggc
    12541 caagatcgat ggtggcgggg tgcaggcgct cgatatagtc gagccagcct gccagatcgg
    12601 tgggaagtga cacgttggcg ctgcttaaac ggtcggtgcg ggaatgcgca tcaacagggt
    12661 gatgagggaa gccagacgtt cgcgcatctg gcgccgatcg acgatgagat cgatggcgcc
    12721 tttttccag
//
LOCUS       DATGOU010000089        14786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_4680212, whole genome shotgun sequence.
ACCESSION   DATGOU010000089 DATGOU010000000
VERSION     DATGOU010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14786
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4680212"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(545..853)
                     /locus_tag="VKZ48_05420"
     CDS             complement(545..853)
                     /locus_tag="VKZ48_05420"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HLU77326.1"
                     /translation="MTSTALHFLDDETYIELDELGQASGLAVEQIIELTEYGVFEPRR
                     DAGTWLYSARSIVTARRAARLQRDFELDATGVALALAYLERIEALEQRLRELECQLLR
                     "
     gene            complement(850..1788)
                     /locus_tag="VKZ48_05425"
     CDS             complement(850..1788)
                     /locus_tag="VKZ48_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HLU77327.1"
                     /translation="MKYKDYYAALGVERGATQDEIKKAYRKLARKYHPDVSKEADAEE
                     RFKEVAEAYQTLSDPEKRAAYDQLGRYRPGEDFQPSQDWETRFGAGGFEDFDLGDLFA
                     RFGGYSRRSGGGHAGRGGFAMPGQDLEATAVISLEEAAQGTELELNLSAPTMGFGQAA
                     APQRTRVRVPKGAVDGQRLRVRGKGGPGIGGGPAGDLYLNIKLRPHPVFKPAGHDLYV
                     EVPVAPWEAALGAEVEVPTLEGRARVRVRPGARGGQKLRLGGKGLPKPGGGAGDLYCV
                     LQIVTPATLSEEEKALYERLRELSNFDPRGERGGAA"
     gene            complement(1987..2112)
                     /locus_tag="VKZ48_05430"
     CDS             complement(1987..2112)
                     /locus_tag="VKZ48_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77328.1"
                     /translation="MEIVTAVLQLVGVATVAYGLVLAVRNGFSDALTRRTAPKAV"
     gene            complement(2263..2571)
                     /locus_tag="VKZ48_05435"
     CDS             complement(2263..2571)
                     /locus_tag="VKZ48_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77329.1"
                     /translation="MYERRIGWNGKAGTPMNLWQKLVAYAAGAAVLVVMLMMSAVLFA
                     VALTAALIIWARFWWKTRELRKRMREAPTDDPFAAFRHATEPGRVIEGEVVERGDRQS
                     "
     gene            complement(2659..4122)
                     /locus_tag="VKZ48_05440"
     CDS             complement(2659..4122)
                     /locus_tag="VKZ48_05440"
                     /inference="COORDINATES: protein motif:HMM:NF023407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3482 domain-containing protein"
                     /protein_id="HLU77330.1"
                     /translation="MNGEQIHLALVAHTNVGKTSLARTLLRRDIGVVRDEPHVTDAAE
                     GQTLIETPGGDALLLWDTPGFGDSARLLKRLRASGNPLGWLLTQVWDRIVDRPFYCSQ
                     QAIRAARDRSDVILYLVNASEDPASAAYVEMEMQILAWLDKPVLLLLNQTGAPRPAEA
                     EAAEATAWREHLRSFDCVRGALTLDAFMRCWVQEDELLGAVQEVLPPAKQPAFGRLRA
                     AWRARNLEIYTAAAAILSEQLARLAADREIVSATGLRERAERWLGTVVRGGQADAAEE
                     QAMRRLAQRLDAAVLEATERLIALHGLSGQAAGEIHARMTEQFRVTTPADVGKTSLLG
                     GIATGALGGLTADLAAGGLTFGAGALIGGVLGAMGAGGAAHAYNLARGTETGQVAWSE
                     DFLTARCESALLRYLAVAHFGRGRGDWRAGEYPAHWSTVCRDVVAPHQAVLYEIWQSL
                     SAQPPDPFARDRLRLLVQELMTDALVRLYPASRALFD"
     gene            complement(4119..5648)
                     /locus_tag="VKZ48_05445"
     CDS             complement(4119..5648)
                     /locus_tag="VKZ48_05445"
                     /inference="COORDINATES: protein motif:HMM:NF022518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HLU77331.1"
                     /translation="MREKALRSVLLMKAIEEADQAGALVPPADRAAATRASTRAQEGA
                     AIPAAPGPLDAAARNLLTARADHLLAQVVRRYPFVGALREQRGVAWAGALLVVLSLLG
                     GFGLSALDGTRTINILSFPLLGLILWNVAVYAVVLYNAVRSLRHEPLHQSLLADMLAS
                     AALRRIRRLIEKSATYNTVLADALGRFLSEWSQTARPLLVARAGTILHLCAAAVGVGL
                     IAGLYLRGIAFDYRAGWESTFLTADTVRALLAFVYAPASALTGIAVPDATHIAAIRLV
                     DGQGGESAANWIHLLAATALLFVVLPRLVLTLLQMSAVLRHALHVKVPASLSAYYRAA
                     FGQVAGGHQLVSVLPYNYEPFGGATAALRALLTTAFGESLVITLHANTRYGEEEDFAR
                     QLAVRHDEAANVVALLFNLAATPEEENHGQVITAARDWLQQTGSEARLLALVDERPYA
                     SRLGPDNARRLNERRETWRRFIAAHGLEACFVALGEDDETMNDARASQLRAAAWQARP
                     A"
     gene            5954..6241
                     /gene="groES"
                     /locus_tag="VKZ48_05450"
     CDS             5954..6241
                     /gene="groES"
                     /locus_tag="VKZ48_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HLU77332.1"
                     /translation="MKIRPLHDRVIVKRMEEERKTASGIVIPDNAAEKPDQGEVIAVG
                     NGKVMDDGKVRPLEVKVGDRVLFGKYSGQAVKVEGEELLVMREEDIMGVIA"
     gene            6272..7924
                     /gene="groL"
                     /locus_tag="VKZ48_05455"
     CDS             6272..7924
                     /gene="groL"
                     /locus_tag="VKZ48_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185913.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HLU77333.1"
                     /translation="MAAKDVRFHEEARHKMLNGVNVLANAVKSTLGPKGRNVVLDRSF
                     GAPTVTKDGVSVAKEIELKDKLENMGAQMVKEVASKTSDVAGDGTTTATVLAQAIVRE
                     GMKFVAAGMNPMDLKRGIDKAVGAIVDELKKISKPCTTSKEIAQVGSISANSDADIGK
                     IIADAMDKVGKEGVITVEDGKSLNNELDVVEGMQFDRGYLSPYFINNPDRQIAVLEDP
                     YVLLHDKKISNIRDLLPVLEQVAKAGRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLEDIAILTGGTVISEEVGLTLEKATLNDLGQAKRVEVGKEETTVID
                     GAGEGKNIEARVKQIRAQIEEATSDYDREKLQERVAKLAGGVAVIRVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALLRARAAIDNLKADHADQEAGIKIVYRALEEP
                     LRTIVANAGDEPSVVVNKVLEGKGNYGFNAATEEYGDLVEMGVLDPTKVTRTALQNAA
                     SVASLLLTTDVAVTELVEDKPAGGMPGGMGGMGGMGGMGMDM"
     gene            8626..8701
                     /locus_tag="VKZ48_05460"
     tRNA            8626..8701
                     /locus_tag="VKZ48_05460"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8659..8661,aa:Phe,seq:gaa)
     gene            8692..8859
                     /locus_tag="VKZ48_05465"
     CDS             8692..8859
                     /locus_tag="VKZ48_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77334.1"
                     /translation="MATIDFAERSEAKSNQGGWSLATMIFCRRQKITQVSNPTPIDFA
                     ERSEAKSNQGG"
     gene            8960..10471
                     /locus_tag="VKZ48_05470"
     CDS             8960..10471
                     /locus_tag="VKZ48_05470"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HLU77335.1"
                     /translation="MTVPFDPERRRVLLAGAGMLLLPAGVHAQILAPSVAPFSLGVAS
                     GWPSATSVVLWTRLTSALTELHASDRMPVGWELAADADFKAVVARGEAMAEREWAHSV
                     HVEVAGLDPDRWYWYRFHAQGQRSPVGRTRTLPEATARPAHLRMVFTSCQHYEGGWYG
                     AHRHIAAEEPDFVIFLGDYIYETSYRHAVRRHPERTEIQTLDEYRRRYACYKGDPDLQ
                     AGHAACPWLVAWDDHEVRNNYPGHLPLEYLLPRRAAAYQAFWEHMPLPERLRPDGPTM
                     RRLYTHFDFGDLVRICMLDTRQYRSPQLTASQVHAESDRLLNDPTRTMLGEAQEAWLH
                     ARLGTSSAIWNVVAQQTIVSRSGAGKHASYRIASDRWDGYPAARGRLMDALRSTANPV
                     VIGGDVHSFWVGELTEDLYDPRTPPVATELVSSSVSSVGPSARTVARRIASNPQMRYG
                     NGAWRGYVRLDIRREHIDAALRAVYDVTSPDTGAFTLATFLNMADIPGVVRTS"
     gene            10880..>14786
                     /locus_tag="VKZ48_05475"
     CDS             10880..>14786
                     /locus_tag="VKZ48_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HLU77336.1"
                     /translation="MAFRFPGDVSDEAGLWEALEQKRDLVTQIPADRWAVDELQHPTR
                     AEPGRSITFSAGVLSRIDEFDAGFFGISPREATWIDPQQRLLLELSWEAMENAGVPPS
                     SMAGSDCAVYVGIASVDYGSRTIDDLASISSHSMTGNTLSIAANRLSYVFDLHGPSLP
                     VDTACSSSLAALHQACNTLRAGEASAALVAGVNVLLHPYPFIGFTKASMLSADGRCKV
                     FDASGDGYVRAEGGAVLLLKPLDAALADGDEIHAVILASGVNADGARKTGITIPSSEG
                     QAELMRDVLARSGIGADEVDFIEAHGTGTAVGDPIETAAIGAVYGQGRATPLPIGSVK
                     ANMGHLEAAAGMAGLVKAVLALKNRALPAALHLDTPNPYIDFEALNLELVTEYKTLEK
                     ADGKPLVAGVNSFGFGGANAHVLLQEFRAPDETKEGLIKPFDKLRANGKGPFVVSREP
                     VERSNHEREPLNHNSHHLPALDALPPLFLSARTDEALKAMAGRYAELLDGRSAQGFYD
                     IAHAAAFRRDRMEKRLALQADSVESAAHLLRSYAEGDAPPEVFIEDSLPQEGSVAFIY
                     SGNGAQWVGMGRALLDESPRFAEILADLDMAMRPDAGFALIEALAADEEHSRLDDTAV
                     AQPLLFAIQVAVTLLMRESGIEPAAVAGHSVGEVAAAWASGALDLEQAIRVICVRSRA
                     QHATRGEGRMAAMRMSPADAAELLAALDECDAEIAGINSPNNVTLSGSLEGLMRVQEA
                     AEAQGIFFRLLDLDYAFHSNRMDPIETPLREQLADLFPLQTETTRFVSAVTGDVLDGR
                     ALDADYWWRNVREPVRFADAVAKLAELGCRVFVEIGPHAILQRYIGECLNAADIKARV
                     LPTLRRTDDGARAITEAVLRAQLVVDCPAFQTWFPYPARRVRLPNYPWQRERHWYPRT
                     AEASLGIERRRVHPLLGWRLNGAEAAWENTLDPAVVPWLADHQVGGTVVYPGAAYAEM
                     ALAAAREWLGGERCIIEELDIVAPMVFDGGQSRTLRFVLDPRDGGFRIKSRRRLSSDE
                     WTLHAVGRVLEPTERIPAARIDLPVAPTRPFEHEAHYRLAAMLGLDYGPAFRQLREGY
                     VGADKLEARLGRIDGEHLEGYLLHPALLDACFQSLIGFFGDEIEMGHGIALLPVKIGR
                     LDRCGEGQAVHLRAHLRRCGTRSVLADFELLDEGNRLVASISGCRFRAAPLKRQAQHK
                     VSKWRIVLRLRPHPLDGLITEVPATADLAAQAHSRLADIQHSRQTWFHETLPLIEALT
                     LSFVCEAFRQLARTQPSDWQQRVQASPLGRWAATLMTEEG"
BASE COUNT         2568 a         4851 c         4842 g         2525 t
ORIGIN      
        1 gttgaaggcc accagcggcc gcgggccatc gacgtcctgc accatcagcg ccacgctgtc
       61 gccgggaatg ccggcgcgct ggagcgcgcg tgccaccgga gcgggaaggg tcgtctgcgc
      121 tgcggcggcg aaggaacaac aaaggaggag cagcgcggcg acgagacggc ggatcatgcg
      181 gagcgtgaaa accagggtgg cccaagtatg ccgtaaaaga ctttcaccac agagacacag
      241 agtacacaga gaaaccttgt cttcaatgct ttctctgtga tctccgtggt ctccgtgttt
      301 cagtcttttc gcgtttcaag tgtcgtactg gatgcccgat cacgcggttg cgccgctgtc
      361 gggaatgacg atggatgagt cctcgccggt catttgccgt tcttggttcc ttggtgtctt
      421 ggtgtcttgg tggttaaaga tttaaagctt ttcgacgctt cgaccgttgg tgctgagcgc
      481 tcgccgcagg cgagcagtcg aagcatgaac ggcgctattg gttcaccccc ttcgtcggag
      541 tcgcctaacg cagcaactgg cactccagtt cgcgcagccg ctgctccagg gcttcgatgc
      601 gctcgagata ggccagtgcg agcgcgacac ccgtggcgtc gagttcgaag tcgcgctgca
      661 ggcgcgctgc acgccgcgcc gtgacgatgc tgcgcgcgct gtagagccaa gtgccggcgt
      721 cgcggcgcgg ctcgaacacg ccgtattcgg tcaactcgat gatctgctcg accgccagcc
      781 ccgatgcctg gccgagctcg tcgagttcga tataagtctc gtcgtcgaga aaatgcaaag
      841 cggtggacgt catgccgcgc ctccacgttc gccgcgcgga tcgaagttcg acagttcgcg
      901 cagccgctca tacagcgcct tctcttcctc cgacagtgtc gcgggcgtaa cgatctgcag
      961 tacgcaatac aggtcgcccg cgccgccgcc cggcttgggc aggcccttgc cgccgaggcg
     1021 cagtttctgt ccgccgcgtg cgcccgggcg tacccgtacg cgtgcacggc cttccaacgt
     1081 cgggacttcc acttccgcgc ccagcgccgc ttcccagggc gcgaccggca cttcgacata
     1141 aaggtcgtga cccgcgggct tgaacaccgg atgcggacgc agcttgatgt tcaggtagag
     1201 gtccccggcc ggaccgccgc cgatgcccgg tccgcccttg ccgcgcacgc gcaggcgctg
     1261 gccgtccacc gcgcccttgg gcacgcgtac gcgcgtgcgc tgcggcgcgg ccgcctggcc
     1321 gaagcccatg gtcggcgcgg acaggttgag ttccagttcg gtgccctgcg cggcctcttc
     1381 caggctgatg accgcggtcg cctccaaatc ctgtccgggc atggcgaaac cgccgcggcc
     1441 ggcatggccg ccgccgctgc ggcggctgta accaccgaag cgcgcgaaca gatcgcccag
     1501 atcgaagtcc tcgaagccgc ccgcaccgaa gcgcgtttcc cagtcctgcg agggctggaa
     1561 gtcctcaccc ggccggtagc ggcccaactg gtcgtaggcg gcccgtttct ccggatcgga
     1621 aagggtttga taggcctccg caacttcctt gaatcgctct tccgcgtccg cttccttcga
     1681 gacgtccgga tggtatttgc gggcgagctt gcggtaggcc ttcttgatct cgtcctgggt
     1741 cgccccgcgc tcgacgccca gggcggcgta gtaatccttg tatttcatgg cagcgcgggt
     1801 gagtgggaca caacggaaac atgcgcgcgc cgtgccgttt tatcaagccg gcgccgaaag
     1861 cccggacggg cggcgcggca gaaaagcaaa aggccggaag tttccggcct tggtcgttga
     1921 atctggcgcc cgctgggggc gccggggtgt cggggctacc cgtttcagga ccccgttact
     1981 gccgctttag acggctttgg gcgccgtacg gcgggtgagc gcatcgctga agccgttgcg
     2041 caccgccagc accagtccat aagcgacggt agcgacacca actaattgca gaacagcggt
     2101 tacgatttcc atttttcgct cctttaccgg cagcaccgtg ctgccttgaa acgaattatg
     2161 gggatgctct tgctgcagtg caaaatgaat tattgcatcg caccataagt aaggtgtatg
     2221 cgtgttgctt atgagccgaa aggtcgctgt gattctccgt gttcagcttt gccggtcacc
     2281 gcgttcgacg acctccccct cgatgacgcg ccccggctcg gtggcgtggc ggaacgcggc
     2341 gaacgggtcg tccgtcggcg cttcgcgcat gcgtttgcgc agttcccgcg tcttccacca
     2401 gaagcgcgcc cagatgatca gggcggcggt gagggcgacg gcgaacagca ccgccgacat
     2461 catcagcatg acgaccagga ccgcagcgcc ggcggcgtag gcgaccagtt tctgccagag
     2521 attcatgggt gtgccggcct tgccgttcca gccgattcgt cgttcgtaca ttcttgtttt
     2581 cctattggga aagcctgaaa agcgttgagg aggttggtgt gcgccgagcc gattcggttc
     2641 cccgcgcgtc gcggtgcgtc agtcgaacag cgcccgcgag gcggggtaga gccgcaccag
     2701 cgcgtcggtc atgagttcct gcacgagcag ccgcagccgg tcccgggcaa aggggtccgg
     2761 cggctgcgcg ctcagcgact gccagatttc gtagagcacc gcctgatgcg gtgccaccac
     2821 gtcgcggcag accgtgctcc agtgcgccgg gtattcgccg gcgcgccagt ctccgcggcc
     2881 gcggccgaaa tgcgccacgg ccaggtagcg caacagcgcg ctctcgcagc gcgcggtcag
     2941 gaagtcctcc gaccaggcga cctgccccgt ttccgttccc cgcgccaggt tgtaggcgtg
     3001 cgccgcgccg cctgcgccca tcgcccccag cacgccgccg atcagcgccc ccgcgccgaa
     3061 ggtgaggccg ccggccgcca gatcggcggt cagtccaccc agcgcgccgg tggcgatgcc
     3121 gcccagcagg ctggtcttgc ccacgtcggc cggcgtcgtg acgcggaact gctccgtcat
     3181 gcgcgcatgg atttcgcccg ccgcctggcc ggacaggccg tgcagggcga tcaatcgctc
     3241 ggtggcttcg agcacggcgg cgtcgagtcg ttgcgccagc cgtcgcatcg cctgttcttc
     3301 ggccgcgtcc gcctgcccgc cgcgcaccac cgtgccgagc cagcgctcgg cgcgttcgcg
     3361 cagacccgtt gcggagacga tctcgcgatc ggcggcgagt cgcgccagtt gctccgagag
     3421 gatcgcggcc gccgcggtat aaatctccag gttgcgcgct cgccaggcgg cgcgcagccg
     3481 cccgaaggcc ggctgcttcg cgggcggcag gacttcctgc accgcgccca gcagttcgtc
     3541 ttcctgcacc cagcagcgca tgaaggcgtc gagcgtcaac gcgccgcgca cgcagtcgaa
     3601 gctgcgcaga tgctcgcgcc atgccgttgc ctccgcggct tccgcctcgg ccggacgcgg
     3661 cgcgccggtc tggttgagca gcagcagcac cggcttgtcg agccaggcga ggatctgcat
     3721 ctccatttcc acataggccg ccgaggccgg atcctccgac gcgttgacca gatagagaat
     3781 cacatcgctg cggtcgcgcg ccgcgcgtat cgcctgctga ctgcagtaga acgggcggtc
     3841 gacgatgcga tcccatacct gcgtcaacag ccagccgagc ggattgccgc tggcacgcag
     3901 gcgcttgagc aggcgtgcgc tgtcgccgaa gccgggcgtg tcccacagca gcagcgcgtc
     3961 gccgccgggc gtctcgatca gcgtctggcc ctcggccgcg tcggtcacgt gcggctcgtc
     4021 gcgcaccacg ccgatgtcgc gccgcagcag cgtgcgcgcg agcgaggtct tgcccacgtt
     4081 ggtatgggcg acgagtgcga gatggatttg ttcgccgttc atgccggacg cgcttgccag
     4141 gcggccgcgc gcagttgcga cgcgcgtgcg tcgttcatgg tttcatcgtc ttcgccgagt
     4201 gcgacgaagc acgcctccag tccgtgcgcc gcaatgaagc ggcgccaggt ctcgcgccgt
     4261 tcgttgagac gacgcgcatt gtccggaccc agacgcgaag cgtagggccg ttcgtccacc
     4321 aacgccaaca ggcgcgcttc gctgcccgtt tgctgcagcc aatcccgtgc cgcagtgatg
     4381 acctgtccgt ggttttcctc ttccggcgtg gcggccagat tgaacagcag cgcgaccacg
     4441 ttggcggcct catcgtgccg cacggccagt tgtcgggcga agtcttcctc ttcgccgtag
     4501 cgcgtgttgg cgtggagggt gatgaccagc gattcgccga acgccgtcgt gagcaatgcg
     4561 cgcagcgccg cggtggcgcc gccgaacggc tcgtagttgt agggcaggac ggagacgagc
     4621 tgatggccgc ccgcgacctg gccgaacgcg gcgcggtaat acgcgctcaa cgaagccggc
     4681 accttcacgt gcagcgcatg gcgcagcacc gccgacattt gcagcagagt cagcacgagc
     4741 cgcggcagga ccacgaacag cagcgcagtg gcggccagca gatgaatcca gtttgccgcg
     4801 ctttcgccgc cctgtccgtc cacgaggcgg atggccgcga tgtgcgtcgc gtccggaacg
     4861 gcgatgccgg tcagcgccga agccggcgca tagacgaaag ccagcagcgc gcgaacggta
     4921 tccgccgtca ggaaggtgct ttcccagccc gcgcggtaat cgaaggcgat gccgcgcagg
     4981 tacaggccgg cgatcaaccc cacacccacg gccgccgcgc acagatggag aatcgtgccg
     5041 gcgcgcgcga ccagcagcgg ccgcgcggtc tgcgaccact ccgacagaaa gcgtcccagc
     5101 gcatcggcga gcaccgtgtt gtaggtcgcc gatttttcga tcagccggcg gatgcggcgc
     5161 agggcggcgg aggccagcat gtcggccagc agggattggt gcagcggctc gtggcgcagt
     5221 gaacggacgg cgttgtagag caccacggcg tacaccgcga cgttccacag aatcaggccc
     5281 agcagcggaa aggacaggat gttgatggtg cgcgtcccgt ccagtgcgga caggccgaaa
     5341 ccgccgagca ggctgagtac gaccagcagc gcgccggccc acgcgacccc gcgctgttcg
     5401 cgcagtgcgc cgacgaaggg gtagcgccgg acgacttgtg ccagcaggtg atcggcgcgc
     5461 gccgtcagca ggttgcgcgc ggcggcgtcc aacggaccgg gggcggcggg aatggccgcg
     5521 ccctcctgtg cgcgggtgct cgcgcgtgtg gctgcggccc gatcggccgg cggaaccagc
     5581 gccccggcct gatccgcctc ctcgatcgct ttcatcaaca ggacgctgcg cagcgccttc
     5641 tcgcgcattt cttcgctttc ggcgcaccgg cgcctggagt cacacacccg gcgattatgc
     5701 ggcctccgag gggccgccgc cggttcgggg aaggcggctc attgacgaac gcgacgccgc
     5761 accgggaccg gaccttgcac aagccgggaa attctctgtg gccgggtctt gaaatgcccg
     5821 atcggcgccc ctattattag cactcgccgc ggccgagtgc taataggccc ggaaaaccct
     5881 ggaatattat ggctgacgct gtgaaggtcg atagcgtccg ctcacttact tattttgttg
     5941 gaggttaagt ggcatgaaga tccgtcccct gcatgaccgc gtgatcgtca agcgcatgga
     6001 agaagagcgc aagactgcca gcggcattgt gattcccgac aacgctgccg agaagcccga
     6061 tcagggtgaa gtcatcgccg tcggtaacgg caaagtgatg gacgacggca aagtgcgtcc
     6121 gctcgaagtg aaagtcggcg accgcgtgct gttcggcaag tattccggcc aggccgtgaa
     6181 ggtcgagggc gaggaactgc tcgtcatgcg cgaagaagac atcatgggcg tcatcgccta
     6241 atcatcggat tcggaatagg agtacctgaa catggcagcc aaagacgtac gttttcacga
     6301 ggaagcacgc cacaagatgc tgaacggcgt gaacgttctc gccaacgccg tcaaatcgac
     6361 gctgggcccc aaaggccgca acgtcgtgct ggaccgttcc ttcggcgccc ccacggtgac
     6421 caaggacggt gtttccgtcg ccaaggaaat cgagctgaag gacaagctcg agaacatggg
     6481 cgcgcaaatg gtgaaggaag tcgcttccaa gacttccgat gtggccggtg acggcaccac
     6541 caccgcgacc gtgctggcgc aggccatcgt gcgtgagggc atgaagttcg ttgccgccgg
     6601 catgaacccg atggacctga agcgcggtat cgacaaggcg gtcggcgcca tcgtcgatga
     6661 gctgaagaag atctccaagc cctgcacgac ctccaaggag atcgcgcaag tcggctccat
     6721 ctccgccaac tccgacgccg acatcggcaa gatcatcgcc gacgcgatgg acaaggtcgg
     6781 caaggaaggc gtcatcaccg ttgaagacgg caagagcttg aacaacgaac tggacgtggt
     6841 cgagggcatg caattcgacc gcggctacct gtcgccctac ttcatcaaca accccgatcg
     6901 tcagatcgcc gtgctggaag atccctacgt gctcctgcac gacaagaaga tctccaacat
     6961 ccgcgacctg ctgcccgtac tggagcaagt ggcgaaggcc ggccgtccgc tgctgatcat
     7021 cgccgaggac gtcgaaggcg aagccctcgc gaccctggtg gtgaacaaca tccgcggcat
     7081 cctgaagacc gtcgccgtca aggctccggg cttcggcgat cgtcgcaagg ccatgctgga
     7141 agacatcgcc atcctgaccg gcggcaccgt gatctccgag gaagtcggcc tgacgctgga
     7201 aaaagccacc ctgaacgacc tcggccaggc caagcgcgtc gaagtcggca aggaagagac
     7261 gaccgtcatc gacggcgcgg gcgaaggcaa gaacatcgaa gcgcgcgtca agcagatccg
     7321 cgcccagatc gaagaggcga ccagcgacta cgatcgcgag aagctgcagg agcgtgtggc
     7381 caagctcgcc ggcggtgtgg cggtgatccg ggtcggcgcc gcgaccgaag tcgagatgaa
     7441 agagaagaag gcccgcgtgg aagacgccct gcacgccacc cgcgccgctg tggaagaagg
     7501 catcgtcccc ggcggtggtg tggccctgct gcgtgcccgc gccgcgatcg acaacctgaa
     7561 ggccgaccac gccgatcagg aagccggcat caagatcgtg taccgcgcgc tggaagagcc
     7621 gctgcgcacc atcgtcgcca acgccggtga cgagccctcc gtggtcgtca acaaggtcct
     7681 ggaaggcaag ggcaactacg gcttcaacgc cgcgaccgaa gagtacggcg acctggtcga
     7741 aatgggcgtg ctcgacccga ccaaagtcac ccgtaccgcg ctgcagaacg ccgcgtccgt
     7801 cgcctcgctg ctcctcacga ccgatgtggc cgtgaccgag ctcgtggaag acaagcccgc
     7861 cggcggcatg cccggtggta tgggcggcat gggcggtatg ggcggcatgg gcatggatat
     7921 gtaaggagcg cgcaagcgtc ctgcattccg gtcccgtacc gaacgacaaa gccccgcaga
     7981 tgcggggctt tgtttttgtg aagggcgcac gcctgaattc cgttcgcccc cgaggttccg
     8041 ttcacccccg aggtttccgt tcgccctgag cgcgagccgc aggcgagcta gtcgaagggt
     8101 gaacgagaga gaacgagggg aaagggaggg gtccgttcat ggttcgactg cgccaagctg
     8161 tcctgagccc gacgaagggc gcgctcacca cgaacggacg atcaccgttc gtcaggagcc
     8221 gcgaaagctg gtccgttcgc cctgagcgcg agccgcaggc gagcagtcga agggtgaacg
     8281 agcatgttgc atcggcatcg tcgtgtgatc ttgccgttca tgcttcgact gcgccaagcc
     8341 tgccctgagc ccgacgaagg gcgcgctcag cacgaacggt cgggtgcccg ttgttctggg
     8401 ttgatcgtga tgcgcaagcg ctggattcag ttggcggaag tggccttcct cgctataatc
     8461 cgcgctcttt gcgcgactcc gtaagtcttg agttgcgact tcgattccag cattgctgaa
     8521 gtcagaaaga cgttgcggcg aaggctaact tcgcggcagc gaagttaagg gcatcgcaag
     8581 atgcccggcc ggaggcaaaa tccttgtcgc cggtgcagta gagatggcca agtagctcag
     8641 ttggtagagc agaggactga aaatccttgt gtcggtggtt cgattccgcc cttggccacc
     8701 atcgatttcg ccgagcgcag cgaggcaaaa tcgaaccaag gtggatggtc cttggccacc
     8761 atgatttttt gccgcaggca aaaaatcaca caggtgtcga atccgacgcc catcgatttc
     8821 gccgagcgca gcgaggcaaa atcgaaccaa ggcgggtagc ccgtggccac cactccccac
     8881 ctttcctaac tcccccgttt tgtcctgagc gttcgttaac gcacaatggc gcgatgcgag
     8941 tcccgctcgt ctgccccttg tgaccgttcc gttcgatccc gaacgtcggc gcgtattgct
     9001 ggccggtgcc ggcatgctgc ttctgcccgc cggcgtgcac gcacaaatcc ttgcgccgtc
     9061 ggtcgcgccg ttctcgctcg gcgtggcgtc cggctggccc tcggcgacga gcgtcgtgct
     9121 gtggacgcgc ctgacgtcgg cgttgaccga gttgcatgca agcgatcgca tgcccgtcgg
     9181 ttgggagctt gcggcggatg cggatttcaa ggcggtggtc gcgcgcggcg aagcgatggc
     9241 cgagcgcgaa tgggcgcaca gcgttcatgt ggaggtcgcc ggcctcgatc cggaccgatg
     9301 gtactggtat cgcttccatg cgcaggggca gcgcagtccg gtcggccgca cgcgcacact
     9361 gccggaagcg acggcgcgtc cggcgcatct gcgcatggtc ttcacttcct gccagcatta
     9421 cgaaggcggc tggtacggcg cgcatcggca catcgccgcc gaagaaccgg acttcgtcat
     9481 ctttctcggc gactacattt acgagacgtc ctatcgccac gcggtgaggc ggcatccgga
     9541 gcgtacggaa attcagacgc tggacgaata ccggcgtcgt tacgcctgct acaagggcga
     9601 tcccgatcta caggccggtc atgcggcctg cccctggttg gtggcatggg acgatcacga
     9661 agtccggaac aattatcccg gccatcttcc actcgaatat ctgttgcctc ggcgcgcggc
     9721 cgcgtatcag gcgttctggg agcacatgcc gctacccgag cgtttgcgtc ctgacgggcc
     9781 gaccatgcgg cgcctttata cgcacttcga tttcggtgac ctcgtgcgta tttgcatgct
     9841 cgatacgcgg cagtaccgtt cgccgcaatt gacggcaagc caggtgcacg ccgaatccga
     9901 tcgcctgctc aacgatccga cgcgaacgat gctgggcgaa gcgcaggaag cgtggctgca
     9961 tgcacggttg ggcacgagtt cggcgatctg gaatgtcgtc gcacagcaaa cgatcgtttc
    10021 gcgttccggc gcaggcaaac atgcgagcta ccgtatcgcg tcggaccgct gggacggcta
    10081 tccggctgcg cgcgggcgac tcatggacgc gttgcgcagc accgccaatc ccgtcgtcat
    10141 cggcggcgac gtccattcct tctgggtggg ggagttgacg gaggatctgt acgacccccg
    10201 cacgccgccc gtcgcaacgg aactggtcag cagttcagtg tcctccgtcg gcccgagcgc
    10261 gcgaaccgtc gcgcggcgca tcgcctcgaa tccgcaaatg cgctacggca acggcgcctg
    10321 gcgcggttat gtgcgtctcg atatccggcg ggagcatatc gacgccgcat tgcgcgccgt
    10381 gtacgacgtg acgagcccgg ataccggtgc atttacgctg gctactttcc tgaacatggc
    10441 cgatataccg ggcgtggtcc gtacaagctg atcaccgttc ccggcaattg ccacgcgccc
    10501 tagcacgact gtctccgaca aaaaagccac gcggcccgat ttcttaccgc gatatatcaa
    10561 acagttcacc cgacaccgcg cacgtaagtg tctaattcgt gacaaaaagg ctgggcggcg
    10621 ttgcgatagg cggctatggt cgcctatgat cgtcacaaaa tttaacaagc ggtggggagg
    10681 gggtcgggga agttgctcac gacctacagg aggcgcccca cctcgagccg ttcgccgcgc
    10741 tgagccgttt tgagcctcgc gagccggggt tccagtgaac aaacaaaagc aacagacatt
    10801 tggccgggag gagccgtccg gggaggcggt actcgaccgt ccaagccgct cttccgccca
    10861 gcagatcgcc atcgtcggaa tggcgttccg gttcccgggc gatgtgagcg atgaggccgg
    10921 cctttgggag gccctcgagc agaagcggga tctcgtcacg cagatcccgg cggaccgctg
    10981 ggcggtggac gaactgcagc accccacgcg cgccgaaccc ggtcgcagca tcactttctc
    11041 cgcaggggtc ctgtcgcgca tcgacgagtt cgatgccggc ttcttcggca tttctccgcg
    11101 cgaagcaacc tggatcgatc cgcagcaacg cctgctgctc gaactgtcct gggaggcgat
    11161 ggagaacgcc ggcgtgccgc cttcctccat ggcgggttcc gattgcgccg tctatgtcgg
    11221 catcgccagt gtcgattacg gctcgcggac catcgatgac cttgcgagca tcagctcgca
    11281 ctcgatgacg ggcaacacgc tcagcattgc cgccaaccgc ctctcctacg ttttcgatct
    11341 gcacgggcct tcgcttcctg tcgacacggc gtgctcgtcc tcattggcgg cgctgcacca
    11401 ggcgtgcaac accctgcgtg ccggagaggc ctcggcggcg ctggtcgccg gcgtgaacgt
    11461 gctgctgcat ccctacccct tcatcggttt caccaaggcc tcgatgcttt ccgccgacgg
    11521 ccgttgcaag gtcttcgatg catccggcga cggctatgta cgagcggaag gcggcgcggt
    11581 gctgttgctc aagccgctgg atgccgccct ggcggacggc gacgaaatcc atgcggtgat
    11641 tctggcgagc ggcgtgaacg ccgacggcgc gcgcaagacc ggcatcacca ttcccagcag
    11701 cgagggtcag gccgaactca tgcgcgacgt tctggcgcgc agcggcatcg gtgccgacga
    11761 agtggacttc atcgaagcgc acggcaccgg caccgccgtc ggcgatccga tcgaaacggc
    11821 cgccatcggc gcggtttacg gacagggccg cgccaccccg ctgcccatcg gctcggtcaa
    11881 agccaacatg ggtcacctgg aagcggctgc gggcatggcc ggcctggtga aggcggtcct
    11941 cgcgctcaaa aaccgggcgc tgcctgccgc cctgcatctg gacacgccga atccgtacat
    12001 cgatttcgaa gcgctcaatc tcgaactcgt caccgaatac aagacgctcg agaaggccga
    12061 cggcaagccg ctggtcgcgg gcgtgaactc cttcggcttc ggcggcgcca acgcgcacgt
    12121 cctgctgcag gagtttcgcg cgccggatga gactaaggag ggtctgatta agcccttcga
    12181 caagctcagg gcgaacggca agggaccgtt cgtggtgagc cgtgagcctg tcgaacggtc
    12241 gaaccatgaa cgggaaccac ttaatcacaa ctcccatcac ttacccgccc tcgatgcgct
    12301 gccgccgctc tttctgtccg cgcgcacgga cgaggcgctg aaggcgatgg ccgggcgcta
    12361 cgccgaactg ctcgacggcc ggtccgcgca aggcttttac gacatcgccc atgccgcggc
    12421 cttccggcgc gatcgcatgg aaaagcgcct ggcgttgcag gccgattccg tcgaatccgc
    12481 agcccatctg ctgcgcagtt atgccgaagg cgatgcgccg ccggaagtct tcatcgaaga
    12541 cagcctgccg caggaaggca gtgtggcatt catctactcg ggcaacggcg cgcagtgggt
    12601 cggcatggga cgcgcgctgc tcgacgagtc gccgcgcttc gcggaaattc tcgccgatct
    12661 cgatatggcg atgcgccccg atgccggctt tgcgctcatc gaggcgctgg cggccgacga
    12721 ggagcattca cggctcgacg acaccgccgt cgcgcagcct ttgttgttcg ccatccaggt
    12781 agcggtcacg ctgctgatgc gcgagtccgg catcgagccc gccgccgtcg cgggtcatag
    12841 cgtcggcgaa gtcgccgcgg cctgggcctc cggcgcgctc gacctggagc aggccattcg
    12901 cgtcatctgc gtgcgcagtc gcgcgcagca tgcgacgcgc ggcgagggcc gcatggccgc
    12961 catgcgcatg tcgcccgccg acgcggcgga actcctcgcc gcgctggacg agtgcgatgc
    13021 ggaaatcgcc ggcatcaaca gcccgaacaa cgtcacgctc tcgggcagcc tggaaggctt
    13081 gatgcgcgtg caggaggcgg ccgaggcgca aggcatcttc ttccggttgc tcgatctcga
    13141 ttacgccttc cacagcaatc ggatggatcc gatcgagacg ccgctgcgcg aacagttggc
    13201 ggatctcttt ccgctgcaaa cggaaacgac gcgcttcgtg tccgccgtga cgggcgacgt
    13261 gctcgacggc cgggcgctcg atgcggacta ctggtggcgc aacgtgcgcg aaccggtgcg
    13321 ctttgccgac gcggtcgcaa agctggccga actcggctgc cgcgtgttcg tcgaaatcgg
    13381 cccgcacgcc atcctgcaac gctatatcgg cgaatgcctg aacgccgccg atatcaaggc
    13441 gcgcgtcctg ccgaccctgc gccgcaccga cgacggtgca cgtgccatca ccgaagcggt
    13501 attgcgcgcg cagttggttg tggactgtcc ggcgttccag acctggtttc cttatcccgc
    13561 gcgacgcgtg cgtctgccga actatccctg gcaacgcgag cgtcattggt atccgcgcac
    13621 cgccgaagcg tcgctcggca tcgagcgtcg ccgcgtgcat ccgctgctgg gttggcgcct
    13681 gaacggcgcc gaagccgcgt gggagaacac gctcgacccg gcggtggtgc cgtggcttgc
    13741 ggatcaccag gtcggcggca ccgtcgttta ccccggtgcg gcctatgcgg aaatggcact
    13801 ggctgccgca cgcgaatggc tgggtggcga gcgctgcatc atcgaagagc tcgacatcgt
    13861 cgcgcccatg gtgttcgacg gcgggcagag ccgcacgctg cgcttcgtgc tcgatccgcg
    13921 cgacggcgga ttccgcatca agagccggcg gcggctcagc agcgacgagt ggaccttgca
    13981 cgcggtcggc cgcgtgttgg aaccgaccga acgcattccc gcagcgcgca tcgatctgcc
    14041 cgtcgcgccg acccggccgt tcgagcacga agcgcactat cgcctcgccg ccatgctcgg
    14101 cctcgattac ggccccgcgt tccggcaact gcgcgagggc tatgtgggcg cggacaaact
    14161 cgaagcgcgg ctgggtcgta tcgacgggga acacctcgag ggctatctgc tgcatcccgc
    14221 gctgctggac gcctgcttcc agtcgctgat cggcttcttc ggcgacgaaa tcgaaatggg
    14281 acacggcatc gcgctcctgc cggtgaagat cggacgcctc gaccgctgcg gcgaaggaca
    14341 ggccgtgcat ctgcgcgccc atctgcgtcg ctgcggcacg cgctccgtgc tcgcggattt
    14401 cgaattgctg gacgagggca accgtctggt cgcttcgatc tccggttgcc gcttccgcgc
    14461 cgcgccgttg aaacggcagg cgcaacacaa ggtgtcgaag tggcgcatcg ttctgcggtt
    14521 gcggccgcac ccgctggacg gtctgatcac cgaagtgccg gcaactgccg acctcgccgc
    14581 gcaagcgcat tcgcgtctgg ccgacattca gcacagccgc cagacctggt tccacgaaac
    14641 gctgccgctc atcgaggcgt tgacgctgtc cttcgtctgc gaagccttcc ggcaactcgc
    14701 ccggacgcag ccgtcggatt ggcagcaacg ggtgcaggcg agtccgttgg gccgctgggc
    14761 agcgaccctc atgaccgaag agggtt
//
LOCUS       DATGOU010000090         8349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_2464934, whole genome shotgun sequence.
ACCESSION   DATGOU010000090 DATGOU010000000
VERSION     DATGOU010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8349
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2464934"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VKZ48_05480"
     CDS             <1..418
                     /locus_tag="VKZ48_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HLU77337.1"
                     /translation="AAKKPAKKVTAAKKPAKKAAVKKAAVKKTAAKKAAPKKKVAAKK
                     KPAAKKKTAAKRKPNPAFMKAMTPSPALAAVIGATPQPRTEVTKKIWAYIKKNKLQDS
                     VNKRNINADDKLKEIFGGKKQVSMFEMTKLVNKHLK"
     gene            812..1696
                     /locus_tag="VKZ48_05485"
     CDS             812..1696
                     /locus_tag="VKZ48_05485"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HLU77338.1"
                     /translation="MSSSPSIGFIGTGRVANTLARALSQAGFNVVALASRTVESAQRL
                     ADALPGSRAYATSQEVADAADLVFLTVPDDTIAPLADALRWRAGMAVVHCSGATELSA
                     LAHAQRAGARVGGFHPLHAFADPLVALAHLPGCAVAVEAEEPLLGELYAVAHALRLAP
                     LRLPPGTRALYHLSGSFAASFIDGLLHEAMELWRERLGVSREQALKALLPLARATLDS
                     VAAVGTVEALTGPISRGDVGTVARHLDALRRDHPQTLPLYALLAQRSVGMAMEKGALT
                     GAQRAELERLLAQAMGKA"
     gene            complement(1693..2562)
                     /gene="htpX"
                     /locus_tag="VKZ48_05490"
     CDS             complement(1693..2562)
                     /gene="htpX"
                     /locus_tag="VKZ48_05490"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018419929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HLU77339.1"
                     /translation="MKRIILFIATNIAVLVVLSIVLRLFGVDGWLSAEGIDYRALLVF
                     SAVLGFTGSIISLLMSKPMAKWSTRAQVIDGSESAETRWLVDTVRRLSERAGIGMPEV
                     AIYDGAPNAFATGALRNSALVAVSTGLLRGMTREEVEAVLGHEIAHVANGDMVTLTLI
                     QGVVNTFVVFLSRIVGYIVDKAVFRTERGTGPGFFITVVICQIVFGLLASMIVMWFSR
                     WREFRADHGSAQLLGSPQPMIAALRRLGGIDRADLPESIRALGINDRPGWMALFSSHP
                     PIEKRIAALQAGG"
     gene            2742..3764
                     /locus_tag="VKZ48_05495"
     CDS             2742..3764
                     /locus_tag="VKZ48_05495"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HLU77340.1"
                     /translation="MKDPVPPVEADAPETDVAVEATLAERLELITRLARRLFHAPIVL
                     LSLLDEGALRVAACQGLPMSALGCELGIDRATLAAGGPLIVSDTQNDPRFNTHRLVAG
                     RPHVRFYAGCLLLGADGAVAGVLSVCDRQPEALDEGDLIQLRDLARMAETALRVARLS
                     EREHTLERERERMRRNALLDTTTNLWNRHAMFELLDREFHRARRAREAVALIMVELDD
                     YDAIAAQYGIAGARTALNEAAQAVRTIIRRSDTVARFGPSELLVFLGRCQTDAAVQLA
                     ERMQQRARKAGITLNGNGMPINLSIGVAGAPAGGDWTPDGLVRSAEEALRTAQKMPRG
                     PRIAVR"
     gene            3846..4229
                     /locus_tag="VKZ48_05500"
     CDS             3846..4229
                     /locus_tag="VKZ48_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77341.1"
                     /translation="MALRIPLVLIVVVAVFYFVFWVPFSFTSLHGTPIPALGSLACAL
                     VAGWFVWKATDGEVRGVLRSTAFGAAVVGGIGFVGGFFGPLIFAPGANQGPLLGLFIT
                     GPLGFLLGGVGGFVYGLMRRGEPAE"
     gene            complement(4278..5948)
                     /gene="leuA"
                     /locus_tag="VKZ48_05505"
     CDS             complement(4278..5948)
                     /gene="leuA"
                     /locus_tag="VKZ48_05505"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HLU77342.1"
                     /translation="MLKNPSVKYRPFPPVDLPDRSWPSKTITKAPVWCSSDLRDGNQA
                     LIEPMDGERKRRMFDLLVQIGFKEIEVAFPSASQTDFDFVRSLIESDAIPDDVTIQVL
                     TQAREDLIRRTFESLRGAKRAIVHLYNATSPLFRRVVFNTDVAGVKDIAVRHAKLFKE
                     LAAQQPETEFRFEYSPETFTATELPVALEVCNAVLDVWQPTPDRKAIINLPATVEVAT
                     PNVYADQIEWMHRRLKYRDSIMLSLHPHNDRGCGIAAAELGQMAGADRVEGCLFGNGE
                     RTGNVDIVTLALNLYTQGIDPELDFSDMNTVAHTVEHCNQLPIHPRHPYVGDLVFTAF
                     SGSHQDAIKKGFAAQQPDQPWEIPYLPLDPADVGRTYESVIRVNSQSGKGGIAFLLER
                     DYGIVLPRRLQIEFSHVVQQVTDGTGKELTATEIWDIFRRTYLAPDAPYAYVSHQSIT
                     EGEGRGIVATVKIDGVERTLRGAGNGPIDAFVHALGLGLTVHNFEERSLGKGSDAKAV
                     AYVEMAGPDGVVIHGVGIHADIVTASLKAIVSAANRLQATGAKKAAAA"
     gene            complement(6303..7046)
                     /locus_tag="VKZ48_05510"
     CDS             complement(6303..7046)
                     /locus_tag="VKZ48_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77343.1"
                     /translation="MDAYGKGRATGYGRLWALALAALLGACATNSPPHRDGAPAERTF
                     TLSDLAKSEIDKVAEAHLDHSMASLRLVMGKLYRRNPRELAKGVAPTADAAIARAFDP
                     GLAWRFPELEGKYGTDALHLAFAPEYTGDRVFAFIAGLGGMIHQAYNGQQEFHLFDQL
                     DPQNLYNAARNVEIAVWKLSTARDAGNELLLLSNEAGPDVRNLSFEREFGKLIAYQDL
                     LARLLAQRSNRTLRFVVQNAASAVFLPIH"
     gene            7190..>8349
                     /locus_tag="VKZ48_05515"
     CDS             7190..>8349
                     /locus_tag="VKZ48_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HLU77344.1"
                     /translation="MLPSIEQRLANELSVKPQQIAAAVALLDEGATVPFIARYRKEVT
                     GGLDDTQLRTLEERLGYLRELEERRATVLASIEEQGKLTPALKAQIDAAETKQRLEDL
                     YLPYKPKRRTKAQIAREAGLEGLAEALLADPTLNPEEEAAKYLKPAFTTDNGDNPGVP
                     DVKAALEGARQILMERFAEDAELIGGLRDYLQENGVLVSTVADGKENEGAKFRDYFQY
                     SEPIKDIPSHRALALFRGRKEEVLRLALKLPEELDIDVKPSAAPFNTCERRIAARFNV
                     ADKGRAADTWLLQCVRWTWQVKVSWQLESDLFNALRERAEEEAIRVFARNLHDLLLAA
                     PAGPRATIGLDPGLRTGVKVAVVDRTGKLLDTATIYPHEPRKDWDGSIATLAA"
BASE COUNT         1480 a         2646 c         2835 g         1388 t
ORIGIN      
        1 tgctgccaag aagcctgcaa aaaaggtcac cgcagccaag aagcctgcta aaaaagcggc
       61 agtgaaaaag gcagccgtga agaagacggc agccaagaag gcggcgccga agaagaaagt
      121 agcggcgaag aaaaagcctg cggcgaagaa gaagaccgct gccaagcgca agcccaatcc
      181 ggccttcatg aaggccatga cgccgagccc tgcgctcgcg gctgtgatcg gcgcgactcc
      241 tcagccccgg acggaagtca ccaagaagat ctgggcctac atcaagaaga acaagctgca
      301 ggattcggtc aacaagcgca acatcaacgc cgacgacaag ctgaaggaaa tcttcggcgg
      361 caagaagcag gtgtcgatgt tcgagatgac caagctggtc aacaagcacc tcaagtaagc
      421 gacacgacag tagggagaac ggccggtgcg gcgcaagccc accggccgtt tttcgttgag
      481 tcgagagctg agagccgaga gcagagagtc gagagtcgtt ggttcgacgc ggtcgcttcg
      541 cgaccggctc accatgaacg gaaagccgga ggcaggcgga aggaacgacc tggacccccg
      601 cttccgcggg agtgacggca aaaccacaac cctgtccacc gtcattcccg acagcggcgt
      661 agccgcgtga tcgggaatcc ggaacggtga ttactccgga ttccgggtcg cgctgcgatg
      721 cagcgttcgt aaccgtcgta tccatccctg cgggaacgga cactcgcccg tcgcctagaa
      781 tggcagcgta cgaataacgc cgagcgctcc catgtcttcc tcgccttcca tcggtttcat
      841 cggcaccggc cgggtcgcca acacgctggc gcgcgcattg agtcaggcgg gtttcaatgt
      901 cgtcgccctg gcaagccgca ccgtcgaatc ggcacaacgt ctggccgatg cgctgcccgg
      961 cagccgcgcc tatgcgacgt cgcaggaggt ggcggacgcc gccgatctcg tctttctcac
     1021 cgtaccggac gacaccatcg caccgctcgc cgatgcgctt cgctggcgcg ccggcatggc
     1081 ggtcgtgcat tgcagcggcg ccaccgagtt gtcggcgttg gcccatgcgc aacgcgccgg
     1141 tgcgcgggtc ggcggctttc atcccctgca tgccttcgcc gatccgttgg tcgcgcttgc
     1201 gcatctgccg ggctgtgcgg tcgcggtgga agcggaagag cctttgctcg gcgagttgta
     1261 cgccgtcgcc catgcgctgc gcctcgcgcc tttgcgtctg ccgcccggta cgcgtgcgct
     1321 gtatcacctg tccggcagct ttgccgcctc gttcatcgac ggtctgctgc acgaggcaat
     1381 ggaactgtgg cgcgagcgtc tgggcgtgtc gcgcgagcag gcgctgaagg cgctgttgcc
     1441 gctggcgcgg gcgacgctgg attcggtcgc ggccgtgggc accgtggagg cgttgacggg
     1501 gccgatatcg cgcggcgacg tgggcacggt ggcgcgtcac ctcgatgcgc tgcggcgcga
     1561 tcacccgcag acgttgccgc tttatgcgct gctcgcgcag cgctcggtcg ggatggcgat
     1621 ggagaagggc gcgctgaccg gggcgcagcg cgccgaactg gaacgcctgc tcgcgcaggc
     1681 gatgggcaag gcttagccgc cggcctgcag cgcggcgatg cgcttctcga tcggcgggtg
     1741 gctggagaac agcgccatcc agcccggacg gtcgttgatg cccagcgcac ggatcgattc
     1801 cggcagatcg gcgcgatcga tgccgcccag acgacgcagc gccgcgatca tcggttgcgg
     1861 agagccgagc aactgcgccg agccgtggtc ggcgcggaac tcgcgccagc gcgagaacca
     1921 catcacgatc atcgaggcga gcaacccgaa gacgatctgg cagatcacca ccgtgatgaa
     1981 gaagccgggg cccgtgccac gttcggtgcg gaacaccgcc ttgtcgacga tgtagccgac
     2041 gatacgcgac aggaagacga cgaaggtgtt caccacgccc tggatcagcg tgagcgtcac
     2101 catgtcgccg ttggcgacgt gcgcgatttc atggcccagc accgcttcga cctcctcgcg
     2161 cgtcatgccg cgcaacaggc cggtggagac ggcgaccagc gcggaattcc tgagcgcgcc
     2221 ggtggcgaag gcattgggcg cgccgtcgta gatggcgacc tcgggcatgc cgatcccggc
     2281 gcgctccgac aggcggcgca ccgtatccac cagccagcgc gtctccgcgc tttcgctgcc
     2341 gtcgatcacc tgcgcgcgtg tggaccactt ggccatcggt ttggacatca gcagggagat
     2401 gatcgagccg gtgaagccga gcaccgccga gaacaccagc aaggcccggt aatcgatgcc
     2461 ttcggccgac agccagccgt ccacgccgaa cagccgcagc acgatgctga gcaccaccag
     2521 tacggcgata ttggtggcga tgaacaaaat gatccgcttc atgaaatttc cccgcgtggg
     2581 acgctgccga gcggattgta ccggcagtga aacgcggcat tggtcaagct tggcggccct
     2641 gtccgctgga caagcgccgg gtgcctgccc atcatggccg gcatgcaagc gggtcgacca
     2701 gggaggtccg ccgtgcgcct tcaagaaaaa aaaccatgtt catgaaagat cccgtgccgc
     2761 cggtcgaggc agacgcgccg gagaccgacg tcgccgtcga agccacgttg gccgaacgcc
     2821 tcgagctgat cacccggctg gcgcgccgtc tgttccacgc acccatcgtg ctcctttcgt
     2881 tgctggatga gggcgcgttg cgcgtggcgg cgtgccaggg cttgcccatg agcgcgctgg
     2941 gctgcgagct gggaatcgat cgcgccacgc tcgccgcggg cggaccgctg atcgtgtccg
     3001 acacgcagaa cgacccccgc ttcaataccc atcgtctggt cgccggccgt ccgcatgtgc
     3061 gcttctacgc cggatgtctg ttgctgggcg ccgacggcgc cgtcgccggc gtgctgagcg
     3121 tctgcgaccg ccagccggag gcgctggacg agggcgatct catacagttg cgcgacctcg
     3181 cgcgcatggc ggaaacggcg ctgcgcgtcg cgcgtctgag cgagcgcgag cacacgctcg
     3241 agcgcgaacg cgagcgcatg cgtcgcaacg cgctgctcga tacgacgacc aatctgtgga
     3301 accgccacgc catgttcgaa ctgctggacc gcgaattcca tcgcgcccgg cgcgcgcgcg
     3361 aagcggtggc gctgatcatg gtggaactgg acgactacga tgccattgcc gcgcaatacg
     3421 gcattgcggg cgcgcgcacc gcgctgaacg aagcggcgca ggcggtgcgc accatcatcc
     3481 gccgttcgga tacggtggcg cgcttcggtc cgtccgaact gctggtcttt ctcgggcgct
     3541 gccagacaga tgcggcggtg caactggcag agcgcatgca gcagcgtgcg cgcaaggccg
     3601 gcatcacgct gaacggcaac ggcatgccga tcaacctgtc catcggcgtg gccggcgccc
     3661 cggccggcgg cgactggacg cccgacggcc tggtgcgcag cgcagaggaa gcattacgca
     3721 cggcgcagaa aatgccgcgt gggccgagga tcgcagtgcg gtagaaaagc agtcgagagc
     3781 cgagagcgaa aagcgaggcg cagaagtccg tttacgaccc cggcagggag gagggaagta
     3841 tgaacatggc tttgcgcatt ccgctcgtgc tgatcgtggt ggtcgcggtc ttctacttcg
     3901 tgttctgggt gccgttctcg ttcacgtcgc tgcatggcac tcccattccg gcgctcggtt
     3961 cgctggcctg tgcgctcgtc gcggggtggt ttgtgtggaa ggcgaccgac ggcgaggtgc
     4021 gcggcgttct tcgcagcacc gccttcggcg ccgccgtggt cggcggcatc ggcttcgtcg
     4081 gcggcttttt cgggccgctg atctttgccc ccggcgcgaa tcagggcccc cttctgggcc
     4141 tgttcatcac cgggccgctg ggatttctcc tgggcggcgt gggcggcttc gtgtacggat
     4201 tgatgcggcg aggcgagccc gcagagtagg aagagcccgg agtacggcgg aaagcccgta
     4261 ctccggattg tggcgtttca ggctgccgcg gctttcttcg cgccggtcgc ctgcagacgg
     4321 ttcgccgccg acacgattgc cttcaacgac gccgtcacga tgtcggcatg gatgccgacg
     4381 ccatggatga ccacgccatc cgggccggcc atttccacat aagcgaccgc cttggcgtcg
     4441 ctgcccttgc cgagcgagcg ttcctcgaag ttgtgcaccg tcagtccaag gcccagcgca
     4501 tggacgaagg cgtcgatggg gccgttgcct gcgccgcgca gggtgcgctc gacgccgtcg
     4561 atcttcaccg tggcgacgat gccgcggcct tcgccttcag tgatgctctg gtgcgacaca
     4621 taggcgtagg gcgcgtcagg cgccaggtag gtgcggcgga agatgtccca gatctcggtg
     4681 gcggtcagtt ccttgccggt accgtcggtc acttgctgca ccacgtggct gaactcgatc
     4741 tgcaggcgac gcggcagcac gatgccgtag tcgcgctcca gcaggaaggc gatgccgccc
     4801 ttgcccgact ggctgttcac gcgaatgacc gactcgtagg tgcggcccac gtccgccggg
     4861 tcgagcggca gataggggat ctcccacggc tgatccggct gctgcgcggc gaagcccttc
     4921 ttgatcgcgt cctgatgcga cccggagaac gccgtgaaca ccagatcgcc gacataggga
     4981 tggcgcggat ggatgggcaa ctggttgcag tgctccaccg tgtgcgcgac cgtgttcatg
     5041 tcggagaagt ccaactcggg atcgatgccc tgcgtgtaga gattcaacgc gagggtcacg
     5101 atgtccacgt tgccggtgcg ttcaccgttg ccgaacaggc agccttccac gcgatcggcg
     5161 cccgccatct ggcccagctc ggcggcggcg atgccgcagc cgcggtcgtt atgcggatgc
     5221 aggctcagca tgatgctgtc gcggtacttg aggcgccggt gcatccactc gatctggtcg
     5281 gcatagacgt tcggcgtagc cacttccacc gttgccggaa ggttgatgat cgccttgcga
     5341 tccggtgtcg gctgccacac gtccagcacc gcgttgcaca cctccagcgc cacgggcagt
     5401 tcggtggcgg tgaaggtctc ggggctgtac tcgaagcgga attcggtttc cggctgctgc
     5461 gccgccagtt ctttgaacag cttggcgtgg cgcacggcga tgtccttcac tccggccaca
     5521 tcggtgttga acaccacgcg gcggaacagc ggcgaggtgg cgttgtacag gtgcacgatg
     5581 gcgcgcttcg cgccgcgcag cgactcgaag gtgcggcgga tgaggtcctc gcgcgcctgg
     5641 gtcagcacct ggatggtgac gtcgtccggg atcgcgtcgg attcgatcag gctgcgcacg
     5701 aagtcgaagt cggtctgcga ggccgaggga aacgcgactt cgatttcctt gaagccgatc
     5761 tgcaccagca gatcgaacat gcggcgcttg cgctcgccgt ccatgggctc gatcagcgcc
     5821 tgattgccgt cgcgcagatc ggaggagcac cacacgggcg ccttggtgat ggttttcgag
     5881 ggccaggagc gatcgggcag atcgaccggg gggaaggggc ggtatttgac ggacgggttc
     5941 ttcaacatga tgggttcctt ggcattcgtt tgacgactac gggccggaca tgggcagggc
     6001 gtgcgggcag ccacggattc aaggcccgca agccggtctg cagtcgtagc gaaacgaagg
     6061 aataaagggc ggcaatcagg ttcatgtcgg tctccgtacg gaaataacgg acgctccgcc
     6121 gggtgttaca ccgggcggtg ggaactactc gaattggggg aaaggagtta tctgcgcgcg
     6181 aggcgcagca gcagaccgag cgtcagggag aggacgccgt agaggaaata cgcggtctgg
     6241 tgggacgaaa tcatgcgggc actctagccc tgcttcccgg gcgcgtcaag cccgtaagcc
     6301 attcagtgga tgggaagaaa caccgcactg gcggcgtttt gcacgacgaa gcgcagtgtg
     6361 cggtttgagc gctgggcgag gaggcgggcg agcaggtcct ggtaggcgat cagcttgccg
     6421 aactcgcgct cgaagctgag gttgcgcaca tccggccccg cttcgttgct cagcagcagc
     6481 aattcattgc cggcgtcgcg ggcggtggag agcttccaca cggcaatttc gacattgcgc
     6541 gcggcgttgt agaggttctg cggatcgagc tggtcgaaca gatggaactc ctgctggccg
     6601 ttgtaggcct ggtggatcat tccgcccaac ccggcgatga aggcgaaaac gcgatcgccc
     6661 gtgtattcgg gagcaaaggc cagatgcagc gcatcggtgc cgtacttgcc ctccagttcg
     6721 ggaaaacgcc aggcgaggcc cggatcgaag gcgcgcgcga tggctgcgtc ggcggtgggc
     6781 gccacgccct tggcgagttc gcgcggatta cggcgataga gcttgcccat caccagtcgc
     6841 agactcgcca tgctgtggtc cagatgcgct tcggcgacct tgtcgatttc ggacttggcg
     6901 agatcggaca gggtgaaggt gcgttcggcg ggcgcaccgt cccggtgggg cggcgagttc
     6961 gtcgcgcagg cgcccaacag cgccgcgagc gccagcgccc acaaacggcc gtagcccgtt
     7021 gccctccctt ttccgtacgc gtccaaacgc cctcttcctc gtactgccgc ggctcttctg
     7081 gtggttgcgc cgcatgcccc gcatgcacgc ttttggccgc acgcgcaagc ttgtcacagc
     7141 ggccgccatt ctgcatagtg cgcggctttt gcaggtccaa gccgttcaca tgttgccttc
     7201 catcgagcag cgcctcgcca acgaactttc cgtcaaaccg caacaaatcg ccgccgccgt
     7261 ggcgctcctc gacgaaggcg ccaccgtgcc cttcatcgcg cgctatcgca aggaagtgac
     7321 cggcgggctc gacgacacgc agttgcgtac gctggaagag cgtctcggct acttgcgcga
     7381 actggaggaa cggcgtgcga cggtgctcgc cagtatcgaa gagcagggca agctcacacc
     7441 cgcgctgaag gcgcagatcg acgccgccga aaccaagcag cggctggaag acctttatct
     7501 gccgtacaag cccaagcgcc gcaccaaggc gcagatcgcg cgtgaagcgg gcctcgaggg
     7561 cctcgccgag gcgctgctgg ccgatcccac gctgaatccc gaggaagagg cggcgaagta
     7621 cctcaagccc gccttcacca ccgacaacgg cgacaacccg ggcgtgccgg atgtgaaagc
     7681 ggcattggaa ggcgcgcgcc agattctgat ggagcgcttt gccgaagacg cggaactgat
     7741 cggcggtctg cgggactatc tgcaggaaaa cggcgtgctc gtgtccaccg tagcggacgg
     7801 caaggagaac gaaggcgcga agtttcgcga ctacttccag tacagcgagc cgatcaagga
     7861 cattccctcg catcgcgcac tggcgctgtt ccgcgggcgc aaggaggaag tcctgcggct
     7921 ggcgctcaag ctgccggaag aattggacat cgatgtcaaa ccgtccgccg cgccgttcaa
     7981 cacctgcgag cggcgcatcg ccgcgcgctt caatgtggcg gacaagggcc gcgccgccga
     8041 cacttggctg ctgcagtgcg tgcgctggac ctggcaggtg aaagtgtcct ggcaactgga
     8101 gagcgatctc ttcaacgcgt tgcgcgaacg cgccgaggaa gaggccatcc gcgtgttcgc
     8161 acgcaatctg cacgatctgc tgcttgccgc gccggccggt ccgcgcgcca ccatcggcct
     8221 cgacccgggg ctgcgcaccg gcgtgaaagt cgcagtggtg gatcgcacgg gcaagctgct
     8281 cgatacggcc accatctatc cgcacgaacc gcgcaaggac tgggacggct cgatcgcgac
     8341 gctcgccgc
//
LOCUS       DATGOU010000091         5073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3806827, whole genome shotgun sequence.
ACCESSION   DATGOU010000091 DATGOU010000000
VERSION     DATGOU010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3806827"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /gene="plsY"
                     /locus_tag="VKZ48_05520"
     CDS             complement(<1..512)
                     /gene="plsY"
                     /locus_tag="VKZ48_05520"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HLU77345.1"
                     /translation="MSLLLLLIAAYLLGSIPFAVVTSKLFGLQDPRKYGSGNPGATNV
                     LRSGNKGAAVLTLLGDSLKGWLAVWAATQLGFDAGQAALAGLAAFLGHVFSAFLRFNG
                     GKGVATALGVLAGIDARIAIFSAVVWLMIAYTSRYSSAAALSAAIAAPFAGLIFLGPT
                     FSVGVLAVIAV"
     gene            694..1068
                     /locus_tag="VKZ48_05525"
     CDS             694..1068
                     /locus_tag="VKZ48_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HLU77346.1"
                     /translation="MDFIFIEEMRVKAWVGIYEREKTGPQTVELNLTFGVPDAATEHD
                     DIADTIDYAVVTARIRRELGERHFNLIESLGEFVVNLLFEEFGAPWVKLKVAKIGVMK
                     DVRRVGVHIQRSRAGVVVPPTA"
     gene            complement(1160..1531)
                     /locus_tag="VKZ48_05530"
     CDS             complement(1160..1531)
                     /locus_tag="VKZ48_05530"
                     /inference="COORDINATES: protein motif:HMM:NF013014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HLU77347.1"
                     /translation="MDLSTYTLPELRRLQAKVEREIRRRSDVTRRNLIKRMQKMAADE
                     GLSLDDLLEGSTPAGAEAKPAAKRGRRPGTKTAKAAKPAPVIKYRNPANPDQGWSGRG
                     RKPQWALDWIAQGKSLDELAV"
     gene            complement(1731..2219)
                     /gene="ybaK"
                     /locus_tag="VKZ48_05535"
     CDS             complement(1731..2219)
                     /gene="ybaK"
                     /locus_tag="VKZ48_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HLU77348.1"
                     /translation="MSRQDTHAPETPATRYLRQHGIAFSSHLYEYEEHGGTAVSAREL
                     NVSEHAVIKTLIMEDENAQPMVVLMHGDHKVSTKELARQIPCKHVEACQPETANRHSG
                     YLVGGTSPFGTRKKMPVFLERTILDLPLIYINGGRRGFLVGIHPHDLLRALQPKLVQV
                     AN"
     gene            complement(2315..3244)
                     /gene="xerD"
                     /locus_tag="VKZ48_05540"
     CDS             complement(2315..3244)
                     /gene="xerD"
                     /locus_tag="VKZ48_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011546052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HLU77349.1"
                     /translation="MSARAPAVESGLQDHHRGELDRFADALWLEHGLARNTLAGYRSD
                     LARFAAWLESRNTRLPAATSADLAAYLAEFSRSAKPASQRRLLATWRRYYRMLLANRE
                     IAEDPTLLLDSPMATERFPKTLSEAQVEALLNAPDTETHQGLRDRCMLEVIYAAGLRV
                     SELVGLKVFAVGLNEGVLRIMGKGSKERLVPIGEIAAEWLQRYLSEGRPALLAGRNCD
                     EVFVTRLGAGMTRQMFWRIIKQHAMDAGIARERISPHTMRHAFATHLLNHGADLRVVQ
                     LLLGHADISTTQIYTHVARERLKQLHAKHHPRA"
     gene            complement(3241..3759)
                     /locus_tag="VKZ48_05545"
     CDS             complement(3241..3759)
                     /locus_tag="VKZ48_05545"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019924600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HLU77350.1"
                     /translation="MASGPGAAGLPTRDDTPPPNFDAIIALPFGPFGIRARHGEVHEL
                     VFLAPGTPLENPRNATAGAAAHALREWIDDPDQPFPVPLARTGTPFRQRVWAAISAIP
                     RGHTATYGALAAQLGSAPRAVGQACGANPFPLIVPCHRVTAANGLGGFANAHDGWLLD
                     VKRWLLAFEEAL"
     gene            complement(3762..4343)
                     /locus_tag="VKZ48_05550"
     CDS             complement(3762..4343)
                     /locus_tag="VKZ48_05550"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HLU77351.1"
                     /translation="MRSFIRTLLAAALLSASIAQAAVQVGGVDFQPELGAQKGMLALN
                     GAGLRTRVGFKVYAMGLYLRAPLTDAAAIIGDKGEKRIRIVLTRDLEAKQFGDALLTG
                     LEKNHEPDELAALKPATDALIGALAAVGEVASGTEILLDQLANGATRLVVNGQQQGHD
                     IADVKFYPALLRIWLGVRPADHDLRDALLRQSK"
     gene            complement(4394..>5073)
                     /locus_tag="VKZ48_05555"
     CDS             complement(4394..>5073)
                     /locus_tag="VKZ48_05555"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HLU77352.1"
                     /translation="VIVPRGGKGLIERISRDARVPVIKHLDGNCHVYIDDEADPVKVV
                     PIVENSKTQRYGTCNTAESLLVARDVADIYLPAIGRMLAAKGVEMRCCAESLALLTDA
                     GIADAKLVEATEADWREEYLAPIIAVKVVAGVDEAIAHINTCSSGHTEAIVTENYSHA
                     MRFLREVDSSSVMVNASTRFADGFEYGLGAEIGISTDKIHARGPVGLEGLTSQKWIVF
                     GNGEIRR"
BASE COUNT          834 a         1524 c         1760 g          955 t
ORIGIN      
        1 acggcaatca ccgccaggac gccgaccgag aacgtcgggc ccaggaaaat cagaccagca
       61 aatggcgccg caatggcagc cgaaagcgcg gcagccgacg aatagcgcga cgtgtaggcg
      121 atcatcagcc agaccaccgc gctgaaaatc gcgatccgtg catcgatacc ggccagtacg
      181 cccagcgcgg tggcgacgcc cttgccgcca ttgaagcgca ggaacgccga gaacacgtgg
      241 ccgaggaagg cggccaggcc ggcgagcgcc gcctggccgg cgtcgaaacc cagttgcgtc
      301 gcagcccata ccgccagcca gcccttgagg ctgtccccga gcagtgtcag caccgcagcg
      361 cccttgttgc cgctgcgcag tacattggtg gcgccggggt tgcccgagcc gtacttgcgc
      421 ggatcctgca ggccgaacag cttgctggtg acgacggcga aaggaatcga gccgagcaga
      481 taggcggcaa tgagcagcag caggagtgac attgtggctc caccgtccgg gcttgccggg
      541 cggctttcaa aaatgaggtt gaacgagggg tttgagcagg ccgcttgcgc tgccgggagc
      601 gctgttcagg ccggcgtggc gaccgtggaa agctttttcc ggcaggcttg ccctggcgcg
      661 ctagaattcc ggcgattcta aacgggaagc agcatggatt tcatcttcat cgaggaaatg
      721 cgcgtcaagg cctgggtcgg catttacgag cgcgaaaaga ccggtccgca gacggtcgag
      781 ctcaatctga ccttcggtgt gcccgacgct gcaaccgagc acgacgatat cgcagacacc
      841 attgattacg ccgtggtcac cgcgcgcatt cgccgtgaac tgggcgaacg ccatttcaac
      901 ctgatcgaaa gcctgggtga attcgtcgtc aatctgctgt tcgaggaatt cggcgcgccc
      961 tgggtgaaat tgaaggtcgc aaagatcggc gtaatgaagg atgttcgtcg cgttggcgtg
     1021 catatccagc gcagccgcgc cggggtggtg gttccgccga ctgcatgaaa aagtaaaggc
     1081 cgcgattccg gacgtgatcc ggattcgcgg ccttttcaat tcgtgtccgg cgcattgatc
     1141 gccgggcgtt gaggtttcgt cagacggcga gttcgtcgag cgacttaccc tgtgcaatcc
     1201 agtcgagcgc ccattgcggc ttgcggccac gaccgctcca gccttgatcc ggattggcgg
     1261 ggttgcggta cttgatgacc ggtgcgggct tggcggcttt ggcggtcttc gtgcccgggc
     1321 ggcggccgcg cttggctgcc ggctttgctt cggcgcctgc cggggtgctg ccctcgagca
     1381 gatcgtcgag cgacagacct tcgtcggcag ccatcttctg catgcgcttg atcaggttac
     1441 gacgcgtcac gtcgctgcga cgacggattt cgcgttcgac cttggcttga aggcggcgca
     1501 gttcgggcag ggtataggtc gacagatcca tggtttctcc ggttttgaaa aggccgttac
     1561 acccctgaaa taggggcata ggcggcgcac atgcaccgca aacaactgcc tgattcgttt
     1621 ggcagcaatt gtgccacttt tcaaaaccgg attgaaaaca attgaatggt ctggattgta
     1681 ctttcagttc cgcagggtgt tccttccatt ctcgcccgat tgcccgcgaa tcaattcgcg
     1741 acctgcacca acttgggctg caaggcgcgc agcaggtcat gcggatgaat acccaccagg
     1801 aatccgcgcc gcccgccgtt gatgtagatg agcggaagat cgaggatggt gcgctcgagg
     1861 aagaccggca tcttcttgcg cgttccaaag ggcgaagtgc cgccgaccag atagccgctg
     1921 tggcgattcg cggtttcggg ctggcaggct tcgacgtgct tgcaggggat ctggcgcgcg
     1981 agctccttgg tcgaaacctt gtggtcaccg tgcatcagga cgaccatcgg ctgggcgttc
     2041 tcgtcctcca tgatcagcgt cttgatcacg gcgtgttcgc tgacgttgag ttcgcgagcg
     2101 gaaaccgcgg tgccgccgtg ttcctcgtat tcgtagaggt ggctggaaaa tgcgattccg
     2161 tgctggcgta gatagcgggt ggccggtgtt tccggtgcgt gcgtgtcttg tctgctcatg
     2221 atgaatccgg attcggtgta attggtctgc tgtcttcggt gaatggagtt caatggtaat
     2281 tgaggctccg ccgtcgattc gcctcggttc ttgttcacgc gcgggggtgg tgcttcgcgt
     2341 gcaactgctt gagccgttcg cgcgccacgt gcgtgtagat ttgcgtggtc gatatgtcgg
     2401 cgtgcccgag cagcaattgc actacgcgca aatccgcgcc gtgattgagc aagtgggtcg
     2461 cgaaggcgtg ccgcatcgtg tgcggtgaaa tccgctcgcg tgcaatcccg gcgtccattg
     2521 cgtgttgctt gatgatgcgc cagaacatct gccgtgtcat gccggcgccg agtcgggtga
     2581 cgaagacttc gtcgcaattg cgcccggcga gcaatgcggg gcggccctcg gacagatagc
     2641 gctgcagcca ctcggcggcg atctcgccaa tgggtacgag gcgctccttg ctgcctttgc
     2701 ccatgatgcg cagcacgccc tcgttgaggc ccactgcaaa taccttcagg ccgacgagtt
     2761 cggatacgcg caggccggcg gcatagatca cctcgagcat gcaccggtcg cgcaggccct
     2821 gatgcgtttc cgtgtcgggg gcgttgagca aggcttcgac ctgcgcctcg ctgagcgtct
     2881 tcgggaaacg ctcggtggcc atcggcgaat cgagcagcag ggtggggtct tcggcgatct
     2941 cgcgattggc gagcagcatg cggtaatagc gccgccaggt ggcgagcagc cggcgctggc
     3001 tggccggttt tgcgctgcgg ctgaattcgg ccagataggc tgcgagatcg gccgatgtcg
     3061 cggcgggcag gcgggtgttg cgcgactcca gccacgccgc aaagcgcgcc aggtcgctgc
     3121 ggtagcccgc cagcgtgttg cgggcgaggc cgtgttccag ccacagggcg tcggcgaagc
     3181 ggtcgagttc gccgcggtga tgatcctgca ggccggactc gaccgccggc gcgcgagcgc
     3241 tcacagcgcc tcttcgaagg ccagcagcca gcgcttgacg tcgagcagcc agccgtcgtg
     3301 ggcattggcg aatccgccca gtccgttcgc ggcggtcacg cggtgacagg gcacgatcag
     3361 cgggaagggg ttggcgccgc aggcctgacc gactgcgcgc ggcgcactgc ccagttgcgc
     3421 ggcaagggcg ccataggtgg ccgtatgccc gcgcggaatc gcgctgatcg cggcccacac
     3481 gcgctggcgg aacggtgtgc cggtgcgcgc gagcgggacc gggaagggct ggtcgggatc
     3541 gtcgatccat tcccgcaacg catgggcggc ggcgcctgcg gtggcgttgc gcggattctc
     3601 gagcggtgtg cccggcgcca ggaacaccag ctcgtgcact tcgccatgac gtgcacggat
     3661 tccgaagggg ccgaagggca gggcgatgat cgcgtcgaag ttgggcggcg gcgtgtcgtc
     3721 gcgcgtgggc aggcccgctg cgcccggtcc gcttgccatg ctcacttcga ctgcctgagc
     3781 aaggcgtcgc gcaggtcgtg gtcggcgggg cgcacgccga gccagatgcg cagcagcgcg
     3841 ggatagaact tcacgtcggc gatgtcgtgg ccctgttgct ggccattgac gaccaggcgt
     3901 gtggcgccgt tggcgagctg gtcgagcagg atctcggtgc cggacgcaac ctcgcccact
     3961 gccgccagcg ccccgatcaa tgcatcggtg gcaggcttga gtgcggcgag ttcgtcgggc
     4021 tcgtggttct tctccaggcc ggtgagcagc gcgtcgccga actgcttcgc ttccaggtca
     4081 cgggtgagca cgatgcggat gcgcttctcg cccttgtcgc cgatgatcgc ggcggcatcg
     4141 gtgagcggcg cgcgcaggta caggcccatc gcatagacct tgaagccgac ccgggtgcgc
     4201 aggccggcgc cgttgagcgc aagcatgccc ttctgggcgc ccagctcggg ctggaagtcg
     4261 acgccaccca cctggacggc ggcctgggcg atgctggccg aaagcagggc ggcggccagc
     4321 agggtacgga tgaagctgcg catcgggttt ccttgtttct acagagtcac gccgcgtccg
     4381 gcggcgccca ggttcagcga cggatctcgc cgttgccgaa cacgatccat ttctggctgg
     4441 tcaggccctc cagcccgacc ggtccgcggg cgtggatctt gtcggtggag atgccgatct
     4501 ccgcgccgag gccgtattcg aagccgtcgg cgaagcgggt cgaggcgttg accatcaccg
     4561 aggacgaatc tacctcgcgt aggaagcgca tggcgtggga atagttctcg gtgacgatcg
     4621 cttcggtgtg gccggagctg caggtgttga tgtgggcgat cgcctcgtcg acgccggcca
     4681 cgaccttcac cgcgatgatc ggggcgagat attcttcgcg ccagtcggcc tcggtcgctt
     4741 ccaccagctt cgcgtcggcg atgccggcat cggtcagcag cgcgagtgat tcggcgcagc
     4801 agcgcatctc cacgcccttg gcggcgagca tgcgaccgat ggcgggcagg tagatgtcgg
     4861 cgacgtcgcg cgcgacaagc agcgattcgg cggtgttgca ggtgccgtag cgctgggttt
     4921 tggaattctc gacgatcggt acgaccttca cgggatcggc ttcgtcgtcg atgtagacgt
     4981 ggcagttgcc gtcgaggtgc ttgatcaccg gcacgcgcgc gtccctggag atgcgttcga
     5041 tcaggccctt gccgccgcgc ggcacgatca cgt
//
LOCUS       DATGOU010000092        17508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_6027723, whole genome shotgun sequence.
ACCESSION   DATGOU010000092 DATGOU010000000
VERSION     DATGOU010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17508
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6027723"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VKZ48_05560"
     CDS             complement(<1..308)
                     /locus_tag="VKZ48_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HLU77353.1"
                     /translation="MRIVGGEWRSRVLTFPDALGLRPTPDRVRETLFNWLGQDLTGKR
                     CLDLYAGSGALGFEALSRGASHVDMVERARPVVAALQRSAAALQAGARLRIVHGDALE
                     "
     gene            458..1582
                     /gene="ftsY"
                     /locus_tag="VKZ48_05565"
     CDS             458..1582
                     /gene="ftsY"
                     /locus_tag="VKZ48_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003269901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HLU77354.1"
                     /translation="MFGLFKRKQTPKSPEAVEAPPAEAVDAPTAEAVAPTEAEAMPVE
                     VAEPSPETTPPALQAEAPVAEPVAAAAARAERASWLGRLRTGLAKTGSQLTGLFGLRK
                     IDEALFEELETALLSADAGVDATMHLLQQLRTRVRKDRLENADQLKEALVEELVKLLH
                     PLEAPPVDVTATRPFIVMIAGVNGAGKTTSIGKLAKLFQSQGLSVLLAAGDTFRAAAR
                     EQLAIWGERNNVTVVAQEGGDPAAVIYDAIHAARARGVDVMIADTAGRLPTQLHLMEE
                     LKKVRRVIGKADPSAPHAVWLVIDANTGQNALAQVKAFDDAIGLTGLILTKLDGTAKG
                     GVVAAIARSRPIPLLYVGVGEGVDDLRPFAAEDFARALLG"
     gene            1588..2247
                     /gene="ftsE"
                     /locus_tag="VKZ48_05570"
     CDS             1588..2247
                     /gene="ftsE"
                     /locus_tag="VKZ48_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HLU77355.1"
                     /translation="MPLISYSQVHKRYPGGYEALRGVSLDVEAGEMLFVTGPSGAGKS
                     TLLKLLPVIERPTGGSVVVNGQNIGKLRPAAIPYVRRNLGLVFQDQKLLYDRTVLDNV
                     TLPMHVCGHAPREAARRARAALDKVGLLHKEKLNPAGLSGGEQQRVCIARAIVNRPAI
                     LIADEPTANLDAQNALHIMEMFRSFNQVGVTVLIASHDQHLVERFASRVVTLDQGRLA
                     A"
     gene            2244..3140
                     /gene="ftsX"
                     /locus_tag="VKZ48_05575"
     CDS             2244..3140
                     /gene="ftsX"
                     /locus_tag="VKZ48_05575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HLU77356.1"
                     /translation="MRTWLQQHAASFGATVRRLARTPLATLLSALAIGASLALPLGAY
                     VLLENLQRVAAHFDGNPQLTVYMALDASPDQTGQIEAQLKSSAAVRDFRWVSRDAALA
                     QMKGSSVGDLLGLLDGNPLPDAYVVNLRTDALAGSEGLAESLRKQAGVSEVQLDTLWI
                     KRLDALLRAGTVVVALLATILAFGLAAVTFNTVRMQILQRRDEYEVAKLVGATDAWVR
                     RPFYYQGALLGLLGAAVALGLLAISSIWLNRELAALGATYSIDLRLSLPPTADLVGVA
                     AFAALLGWLGAHLSVSRHLLAK"
     gene            3202..4074
                     /gene="rpoH"
                     /locus_tag="VKZ48_05580"
     CDS             3202..4074
                     /gene="rpoH"
                     /locus_tag="VKZ48_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013955448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HLU77357.1"
                     /translation="MGGSATMSNALTLNSLPLAVGSLDHYVQTVNRFPLLTAEQEYEL
                     GRRWRNENDLDAARQLVLSHLRVVVAIARGYLGYGLPHADLIQEGNIGLMKAVRRFDP
                     ERGVRLVSFAIHWIKAEIHEYVLRNWRLVRIATTKAQRKLFFNLRSMKPGLESLSPDE
                     VQRVADELRVKPEEVREMEMRMAGQDLGLEPTVDDDEESFAPISYLSDPESEPSRLVE
                     ADQTQRLQSENLSEALDSLDPRSRRIIEARWLRENETATLHELAAEFNVSAERIRQIE
                     AKAMQKMRGLIEAN"
     gene            complement(4135..4713)
                     /locus_tag="VKZ48_05585"
     CDS             complement(4135..4713)
                     /locus_tag="VKZ48_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HLU77358.1"
                     /translation="MRWIAVVLFAALLGACGQEQPEFRNTDITGSAVGDDFRLTDHNG
                     QVRTLADFRGKVVAVFFGFTQCPDVCPTTLMEMKGVRERLGDEGERFQALFITIDPAR
                     DTQQLLAEYVPAFHPSFLGLYGDEAATERTAKAFKVYYQKVPGSTPENYSMDHTAATY
                     IFDPEGRLRLYVRLGAGVEAVAHDVKLLLDGR"
     gene            complement(4713..5609)
                     /gene="cyoE"
                     /locus_tag="VKZ48_05590"
     CDS             complement(4713..5609)
                     /gene="cyoE"
                     /locus_tag="VKZ48_05590"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HLU77359.1"
                     /translation="MTTEALSTASHRFRQFYELTKPRVVSLIVFCAVIGMFLASPPHI
                     MVPLDILVFATVGIALVAGAAAAVNCLVEQGIDAVMARTRARPLPRGELTSTQTVVFA
                     AAVGGLGLWLLHQFVNPLTMWLTLGTFVGYAIIYTIVLKPATPQNIVIGGASGAMPPV
                     LGWAAVTGEVNPEALLLFLIIFAWTPPHFWALALYRTEEYRRAGLPMLPVTHGRQFTA
                     LHVLLYTFILVACTLLPFVYGMSGMIYLLSALVLGGIFLAYAIGLYVRYSDRLARKTF
                     RYSIVYLSLLFAALLVDHYIIV"
     gene            complement(5632..6654)
                     /locus_tag="VKZ48_05595"
     CDS             complement(5632..6654)
                     /locus_tag="VKZ48_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HLU77360.1"
                     /translation="MYRKTIFLAALLTFVVVVVGAYVRLSDAGLGCPDWPGCYGRVTP
                     FHASDQISLAVAEQGGEHGPVSMQKAWKEMFHRYIAGFLGLMLLTIAVIAWTKRTALR
                     QSPALPTVLLAVVVVQATLGMWTVTLLLKPVIVTLHLLGGMTILAMLSWLALRQMNLQ
                     APPEAANLRTPALVGLLLLVLQIALGGWVSSNYAALACPDLPLCRGELMPTMDFANAF
                     HMLRELGMTPQGELLSNEALTAIHWMHRLGALVVTAVTLYLVLRMTALNATRKLGLLL
                     LGALVLQFTLGVLNVVYSLPLLLAAAHNGGAAILLVIYVVINFITHRAAALPDARLAG
                     RLSTQS"
     gene            complement(6657..7250)
                     /locus_tag="VKZ48_05600"
     CDS             complement(6657..7250)
                     /locus_tag="VKZ48_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit I"
                     /protein_id="HLU77361.1"
                     /translation="MTDSSPAQDAARRRTARRYLLTIFAICAAPFVLGTLAFYYFPPS
                     AHTNYGELLSPTPLPDVALTAPEGERTSLAALRGKWLMVQFNESRCDEACERKLYHIR
                     QSRTAQGKHMDRVERVWFVLDGQTPAAPSALYEGSYIGQEVPSAFRAQFPATGDLSEH
                     IYLIDPLGNLMLRFPPNPEPHRIIKDLQRLLQVSRVG"
     gene            complement(7243..7914)
                     /locus_tag="VKZ48_05605"
     CDS             complement(7243..7914)
                     /locus_tag="VKZ48_05605"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HLU77362.1"
                     /translation="MTALTIVFCAVTISAGNWQRSRALEKASLQQKLDAWAAQPPLQV
                     PVTPVDSKDIELRPVVATGRYVVDRTILIDNKVHRGRVGYHVVTPLRLEDSEMHVLVV
                     RGWVVGGATRDQLPVIQTPEGVRQVEGIAAIPSDRVLELAEVQPGYVWQNLLLDRYAA
                     WSKLKLQPFVIRQTNDVADGLVRDWPRPDVGIEKHRIYAMQWYSFATLSLILYVALNL
                     KRNRD"
     gene            8019..8219
                     /locus_tag="VKZ48_05610"
     CDS             8019..8219
                     /locus_tag="VKZ48_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HLU77363.1"
                     /translation="MTYIVLLAFVLIIGSLVSALVFMVRDRGRSKRTVYALALRVGLS
                     LTLFLLLMLSYRMGWITGRLLQ"
     gene            complement(8315..9166)
                     /locus_tag="VKZ48_05615"
     CDS             complement(8315..9166)
                     /locus_tag="VKZ48_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019921382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HLU77364.1"
                     /translation="MVLSNTAKYFVPEPSHWPIVGSVSLFSMALGATIWFNGIGIGGW
                     VLLIGFLGLVSMMFGWFGNVIHESEGGMYNKRVDVSFRWGMSWFIFSEVMFFGAFFGA
                     LYWARAHSVPDLGGIESKLLWPDYGAEWPTAGPGFDTQFTPMSAWGIPALNTLLLLTS
                     GVTVTWAHHALKENNRSQLILGLFLTVALGATFLGFQIYEYMHAYSDLNLKLTSGIFG
                     STFFMLTGFHGFHVTVGAIMLTVILIRSIKGHFGPEHHFAFEAAAWYWHFVDVVWLGL
                     FVVVYWL"
     gene            complement(9255..9806)
                     /locus_tag="VKZ48_05620"
     CDS             complement(9255..9806)
                     /locus_tag="VKZ48_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HLU77365.1"
                     /translation="MSTPTKSAADANKRLMKKLLVVAGMMFGFGFLLVPFYEQICEVT
                     GLRSIVKRDAEPVNTQIDYARTVTIEFDSNTHHLPWDFKPKQRFVTVHPGELTTISYE
                     VRNKQDRPVTGQAVPSFGPQLAGLHFKKMECFCFEQQTLGPGEVREMPVVFLLDADLP
                     KDVNTITLSYTFFEVAGTAGKKG"
     gene            complement(9803..9898)
                     /locus_tag="VKZ48_05625"
     CDS             complement(9803..9898)
                     /locus_tag="VKZ48_05625"
                     /inference="COORDINATES: protein motif:HMM:NF038351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome oxidase small assembly protein"
                     /protein_id="HLU77366.1"
                     /translation="MSKNVKTALILLSIVATFFFGIIFKYWLLGS"
     gene            complement(9926..11515)
                     /gene="ctaD"
                     /locus_tag="VKZ48_05630"
     CDS             complement(9926..11515)
                     /gene="ctaD"
                     /locus_tag="VKZ48_05630"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HLU77367.1"
                     /translation="MSAVLDQHGHAHDHGHHPTGIMRWITTTNHKDIGTLYLWFSFTM
                     FLVGGLLAFGIRAELFVPGMQVWNPEFFNQLTTMHGLIMIFGAIMPAFVGFANWQIPL
                     MIGAPDMAFARMNNWSFWLLPAAATLLIASFFVPGGAAGAGWTMYAPLSTQMGPGQDL
                     AIFAVHILGASSIMGAINIVVTVLNMRAPGMTLMKMPMFVWTWLITAYLLIAVMPVLA
                     GAITMNLTDRHFGTSFFNAAGGGDPVMYQHIFWFFGHPEVYIMILPAFGIISQVVPAF
                     ARKPLFGYSSMVYATASIAILSFIVWAHHMFTVGMPTAGLLFFMYATILIAVPTGVKV
                     FNWVATMWKGSMTFETPMLFAIGFIFVFMVGGFTGLILAIAPVDIQFQDTYYVVAHFH
                     YVLVAGSLFAMFAGAYFWLPKWTGRMYNETLGKWHFWLSMIFFNITFFPMHFLGMAGM
                     PRRIPDYAPQFADYNMVASIGAFGFGLAQLIFLYVALQCAMRKGEKAPDRPWEGADTL
                     EWTHLPSPAPYHSFEEPPVVR"
     gene            complement(11534..12745)
                     /gene="coxB"
                     /locus_tag="VKZ48_05635"
     CDS             complement(11534..12745)
                     /gene="coxB"
                     /locus_tag="VKZ48_05635"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HLU77368.1"
                     /translation="MTIKSRLLPVAGFALALFSGVVCAQQIQPNRHDFQPPVTPVARE
                     IYDLHYLMLAICLVVFFGVLAVMIYSIVKHRKAVGAKAEQFHENTTVEIIWTLVPFLI
                     LVGMAWPATKSVLAIKDTSAPDITIKATGYQWKWGYDYIKGEGEGISFLANLSTPYEQ
                     IYAGAQRGTDYLLETDRELVVPVNKKVRIITTANDVIHAWWVPALGVKQDAVPGFVRD
                     TWFYAEKEGVYRGQCAELCGKDHAFMPIVVRVVSADAYSDWVAKTRQAQGIQPAGVQV
                     AAAETSAPAAAAPAAPAEDLSKEWNKDELMARGEKVYGTTCAVCHQGNGQGTPPMFPA
                     QAGSDIVNGPKGPHIDLVMNGKPGTAMAAFGPQLSDVDLAAVITYTRNSWGNKPAENV
                     VQPAEIKAARK"
     gene            complement(12796..13287)
                     /locus_tag="VKZ48_05640"
     CDS             complement(12796..13287)
                     /locus_tag="VKZ48_05640"
                     /inference="COORDINATES: protein motif:HMM:NF021517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HLU77369.1"
                     /translation="MDDACIKVPASVGVGDFSLTVKRNCSISPQTLLWLLLATVLLSF
                     GIATGFALRGAWLVFPFAGIEMLALAAALYVNGRHAADYERIVRRDGELIVEVGDGER
                     VSIHRFHAGHVRVCAHAGRNGLRIALAESGRELEVGRHLDMPGRKSLARLLRARLAGS
                     KAE"
     gene            complement(13469..14287)
                     /locus_tag="VKZ48_05645"
     CDS             complement(13469..14287)
                     /locus_tag="VKZ48_05645"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLU77370.1"
                     /translation="MLAREIERRLFERLEYIKVTPERVLDIGCGTGLSRIRLRERYPA
                     ADVYAVDWAMPALRHNAASRSLVDRARALFAPRRDFLLAGNAGALPFADRSFDVVWSN
                     LALAWAPDPVAWLREWHRVLKVDGLLMFTTYGPDTLRTLRESFAAVDALPHVHPFVDM
                     HDLGDALVACPFADPVVDMEMLTLTYPDVNALLCELRGTGSRNAHRMRRRTLTGRERW
                     TKMIRAYEAGMAGAGRIAAEFEIIYGHAWKAAPRALSDGRQIITFTPREKRASR"
     gene            complement(14368..15357)
                     /gene="bioB"
                     /locus_tag="VKZ48_05650"
     CDS             complement(14368..15357)
                     /gene="bioB"
                     /locus_tag="VKZ48_05650"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017772527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HLU77371.1"
                     /translation="MPQTASPPSSVVPQAPVQSPRWTRAAVEALYELPFNELIHRAHE
                     VHRANFDPQAIQLSTLLSIKTGGCPEDCGYCPQAARYDTGVESQKLLSVEEVLAAASA
                     AKAKGATRFCMGAAWRGPKQKDLEPVLEMVRAVKSLGLETCATLGLLRDGQAEQLRDA
                     GLDYYNHNIDTAPEFYGEVISTRQYEDRLSTLDKVREAGLHVCCGGIVGMGETRSQRA
                     GMIAQLASMTPPPESVPINNLVPVPGTPLAESQSLDWTEFVRTIAVARITMPKSFVRL
                     SAGRQEMHEAVQAFCFHAGANSIFYGDKLLTTGNPEADADRALLTKLGLSALA"
     gene            15408..16106
                     /locus_tag="VKZ48_05655"
     CDS             15408..16106
                     /locus_tag="VKZ48_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HLU77372.1"
                     /translation="MLGLSPALRRLRSALLAQDCALCAAPSGDALICATCTAELPAMT
                     SACPQCGDASPGDQVCGACLAHPPAYDATIAAWRYDFPLDRLVQALKYGNRLALAAWF
                     AEALAARLDERRVDLVVPLPLHARRLRERGFNQAMEIARRLRKPAGTPPAPNVLVRSA
                     DTAAQTGLSNEARKRNVRNAFIATQSLDGLRVALVDDVMTTGATLDAAARALKNAGAI
                     HVENWVVARAVRND"
     gene            16116..16580
                     /locus_tag="VKZ48_05660"
     CDS             16116..16580
                     /locus_tag="VKZ48_05660"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006763321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HLU77373.1"
                     /translation="MLDIVLYEPEIPPNTGNLIRLAANSGSRLHLVKPLGFSLSERAL
                     KRSGLDYGALASVTVHEDWSACRSHFAGRNCYAVTTRGVRCYADVAYARDDVVVFGPE
                     TRGLPAEVLGQFPDENRLRLPMAAGNRSLNLANAVSIVVYEAWRQADFVGGA"
     gene            complement(16582..>17508)
                     /locus_tag="VKZ48_05665"
     CDS             complement(16582..>17508)
                     /locus_tag="VKZ48_05665"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008334202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HLU77374.1"
                     /translation="ERQHRIGLWTRNDEQRATLRRERQSVYLPEATLPDAIDVVDDLP
                     AALQGADLVLIATPTAGLRETLGLVKNVNQQVPVVWACKGFESDTGLLPHQVVGEVIG
                     TEARCAALSGPSFAMEVARGQPTALTLAAHDEAFARRTAAELHQPLLRTYFSDDLPGV
                     EVGGAVKNVMAIATGVCDGLSLGQNARAALITRGLAELSRLGVAMGGRTETFMGLTGL
                     GDLILTCTGDLSRNRKVGLELGRGDTLPAILKRLGHVAEGVNTAREVERRAAELGVDM
                     PITRAVCDVLFRGVSAREAVEQLLARDPKAES"
BASE COUNT         3401 a         5537 c         5509 g         3061 t
ORIGIN      
        1 aattctaacg catcgccgtg gacgattcgc agtcgcgcgc cggcctgcag ggccgcggcg
       61 ctgcgctgga gggccgcgac gacggggcgc gcgcgctcga ccatgtccac gtggctggcg
      121 ccgcgagaga gcgcctcgaa gccgagtgcg ccggagccgg cgtagagatc caggcaacgc
      181 ttgccggtga gatcctggcc cagccaattg aacagcgtct cgcgcacgcg atcgggcgtc
      241 gggcgcaatc cgagggcatc gggaaaagtc agcacgcggc tgcgccattc gccgccgaca
      301 atgcgcaatc tgtttcgagc ggtaggggcc atcggccgga atcgcctgtg aggcgtctgt
      361 taaaatccca cgccatgtta aggaatgtct gattgtttcg gacacttaat cggcatttct
      421 tcccttcttg cgcgcctgcc gccctccctt catagcgatg ttcggacttt tcaaacgcaa
      481 gcagacaccg aaatcaccgg aggcggtcga ggccccgccg gcagaggcgg tcgatgcccc
      541 cacggcggaa gccgtcgcgc cgaccgaagc cgaagcgatg ccagttgagg tcgccgagcc
      601 gtcgcccgag accacaccgc cggcactgca ggccgaagcg cctgtcgccg agccggtcgc
      661 ggctgcggcg gcgcgcgccg aacgcgcgtc ctggctgggc cgcctgcgta ccgggctggc
      721 gaaaaccggc tcgcaactga ccggcctttt cggcctgcgc aaaatcgatg aggcgctgtt
      781 cgaggaactg gaaaccgcgc tgctctccgc cgatgcaggc gtcgacgcga cgatgcacct
      841 gcttcagcag ttgcgcacgc gcgtgcgcaa ggaccggctg gaaaacgccg accagttgaa
      901 ggaagcgctg gtggaggagc tggtcaagct gctgcatccg ctcgaagcac cgccggtgga
      961 tgtgacggcc acccgaccct tcatcgtgat gatcgccggc gtcaacggcg caggcaaaac
     1021 cacttcgatc ggcaagctcg ccaagctgtt tcagtcgcag ggcctgtcgg tgctgctcgc
     1081 ggcgggcgac actttccgcg ccgcggcgcg cgagcagctg gcgatctggg gcgagcggaa
     1141 caacgtcacc gtcgtcgcgc aggaaggcgg cgaccccgcg gccgtcattt acgacgccat
     1201 ccatgcagcg cgcgcgcgcg gtgttgacgt gatgatcgcc gacaccgcgg gccggctgcc
     1261 gacgcagctg catctgatgg aagagttgaa gaaagtgcgt cgggtgatcg gcaaggccga
     1321 cccctccgca ccgcacgcgg tatggctggt cattgatgcc aacaccgggc agaacgccct
     1381 ggcgcaggtc aaggctttcg acgacgccat cgggctgacc ggtctgatcc tgaccaagct
     1441 cgacggtacc gccaagggcg gcgtggtcgc cgccattgcg cgcagccggc cgattccgct
     1501 gctgtatgtg ggcgtcggcg aaggcgtgga cgatctgcgt cccttcgccg ccgaagattt
     1561 cgcgcgcgcc ctgctcggct gaggctcatg ccgctgatca gctacagtca ggtccataag
     1621 cgctatcccg gcggctatga agcgctgcgc ggcgtgagcc tggatgtcga ggccggcgaa
     1681 atgctgttcg tcaccggccc gtccggtgcc gggaagagca ccctgctcaa gctgctgccg
     1741 gtcatcgagc gcccgaccgg cggctccgtc gtagtgaacg gacagaacat cggcaaactg
     1801 cgccccgcag ccattcccta cgtccggcgc aatctggggc tggtgttcca ggatcagaag
     1861 ctgctctacg accgcaccgt gctcgataac gttacgctgc ccatgcatgt gtgcggccat
     1921 gcaccgcggg aagcggcgcg gcgcgcgcgc gccgcgctcg acaaggtcgg cctgctgcac
     1981 aaggagaagc tcaatccagc cggtctttcg ggcggcgaac agcagcgcgt ctgcatcgcc
     2041 cgtgcgatcg tcaaccggcc ggcgatcctc atcgccgacg aaccgaccgc caacctggac
     2101 gcgcagaatg cgctccacat catggaaatg tttcgctcct tcaatcaggt cggcgtcacg
     2161 gtgctgatcg ccagccacga tcagcatctg gtcgagcgct tcgcgtcgcg cgtcgtcacg
     2221 ctggaccagg gacggttggc cgcatgagga cctggctgca gcaacatgcg gcaagtttcg
     2281 gcgccaccgt gcggcgtctg gcgcgcacgc ccttggccac tttgctcagc gcactggcca
     2341 tcggcgcgag cctcgcattg ccgctcggcg cctatgtgct gctggagaac ctccagcgcg
     2401 tggcggcgca tttcgacggc aatccgcaat tgacggtgta catggcgctg gacgcatcgc
     2461 cggaccagac cggccaaatc gaagcgcagc tcaaatccag cgcggcggtc cgcgacttcc
     2521 gctgggtatc ccgcgacgcc gccctggcac aaatgaaggg gtcgagcgtg ggcgatctgc
     2581 tcggcctgct ggacggcaat ccgctgcccg atgcttatgt cgtcaatctc cgcaccgacg
     2641 cgctggccgg aagcgaaggc ctggccgaat cgctgcggaa gcaggccggc gtcagcgaag
     2701 tgcaactcga tacgctatgg atcaagcgcc tggatgcctt gctgcgagcc ggaacggtcg
     2761 tggtcgcgct actggccacc atcctggcgt tcggtctcgc ggccgtgacc ttcaataccg
     2821 tacggatgca gattcttcag cgccgcgacg agtacgaggt ggcgaagctg gtgggtgcca
     2881 ccgacgcctg ggtgcgccgc ccgttctact atcagggcgc cctccttggc ctgctgggcg
     2941 cggccgtggc gctcggcctg ctggcgatca gcagcatctg gctgaaccgg gaactggcgg
     3001 cactgggcgc gacctattcc attgacctcc gtctgtcgct tccgccgacc gccgatctgg
     3061 tcggtgtggc cgctttcgcc gcattgctgg gctggctggg tgcccatctg tccgtaagca
     3121 ggcacttact ggccaaatga aaggacggga acccctgccg cttttagcac tctcataacc
     3181 agagtgctaa aatcgccgct tatgggaggt tccgcaacga tgagcaacgc tttgactttg
     3241 aattcactgc cgctggccgt cggcagtctg gaccattacg tccagaccgt gaaccgcttt
     3301 ccgctgctga cggcggaaca ggaatatgaa cttggccgca gatggcgtaa cgagaacgat
     3361 ctcgacgcgg cacgccagtt ggtgctgtcg catctgcgcg tggtagtcgc cattgcgcgc
     3421 ggctaccttg gttacggtct gccgcatgcc gacctgattc aggaaggcaa tatcggcctg
     3481 atgaaggcgg tgcgtcgctt cgacccggag cgcggcgtgc gcctggtgtc gttcgccatt
     3541 cattggatca aggcggaaat ccacgaatac gtcttgcgca actggcgtct ggtgcgcatc
     3601 gccaccacga aggcgcagcg caagctgttc ttcaatctgc gcagcatgaa acccgggctg
     3661 gagtcgctgt cgcccgacga agtccaacgc gtggccgacg aactgcgggt caagcccgaa
     3721 gaagtccgcg aaatggaaat gcgcatggcg ggtcaggatt tggggctgga accgaccgtc
     3781 gacgatgacg aagagagttt cgcgcccatc tcctatctca gcgacccgga aagcgagccg
     3841 tcgcgcttgg tcgaggcgga tcagacccag cgcctgcaat ccgagaatct ttcggaagcg
     3901 ctggacagtc tggatccccg cagccgtcgg atcatcgagg cgcgttggct gcgcgagaac
     3961 gaaacggcga cgctgcacga actggccgcc gagttcaacg tctccgccga acgcatacgc
     4021 cagatcgagg ccaaggccat gcagaaaatg cgcggcctga tcgaagcgaa ctaagttcag
     4081 ggattgtgga aaccaaaacg cccggcaatg ccgggcgttt tgcgttgcgg gcgatcagcg
     4141 gccgtcgagc agcaatttga catcgtgggc gaccgcctcc acgccggcgc ccagtcgtac
     4201 gtacaaacgc agccggcctt ccggatcgaa tatataggtc gcggccgtgt ggtccatgct
     4261 gtagttctcg ggtgtgctgc ccggcacctt ctggtagtag accttgaatg ccttggctgt
     4321 gcgctcggta gcggcctcgt cgccataaag acccagaaag ctgggatgga aagcgggcac
     4381 gtattccgcg agcagttgct gcgtatcgcg cgcggggtca atggtgatga acagcgcctg
     4441 aaaccgctcg ccttcatcac ccagccgttc acggacgccc ttcatttcca tcagcgtggt
     4501 gggacagacg tcgggacact gcgtgaagcc gaagaacacc gcgaccacct tgccgcggaa
     4561 atcagccagt gtgcggacct gcccgttgtg atcggtgagc ctgaaatcgt cgcccaccgc
     4621 cgagccggtg atatcagtgt tgcggaactc cggctgttcc tgcccgcagg cgccgagcag
     4681 tgcggcaaat aaaacgacag cgatccagcg cattacacaa tgatgtagtg atcgaccagc
     4741 agcgccgcaa acagcagcga cagatagacg atggagtagc gaaaggtttt gcgtgcgagg
     4801 cggtcactgt agcgcacata caggccgatc gcatacgcga ggaagatgcc gcccagcacc
     4861 agcgcgctga gcagatagat cattccgctc atgccgtaga caaaaggcag cagtgtgcag
     4921 gccacaagga tgaaggtgta cagcaagacg tgcagcgccg tgaactgccg gccgtgcgtg
     4981 accggcagca tcggcaatcc ggcacgccga tactcctccg tacgataaag cgcgagcgcc
     5041 cagaagtgcg gcggcgtcca cgcaaaaata atgaggaaca gcagcagcgc ctcgggattc
     5101 acttcgccgg tcactgccgc ccagccgagt accggcggca tcgcgccgga tgcgccgccg
     5161 atgacgatgt tctgcggcgt agccggcttg agcacgatgg tgtagatgat ggcgtagccg
     5221 acgaaagtcc ccagcgtcag ccacatggtg agcggattga cgaactggtg cagcagccac
     5281 aggcccaagc cgcccactgc ggcggcgaag accaccgtct gcgtggaggt gagttcgccg
     5341 cgcggcagcg gtcgcgcgcg cgtgcgcgcc atgaccgcgt cgatgccctg ctcgaccagg
     5401 cagttcactg cggcggccgc gccggcgacc agcgcaatgc cgaccgtggc gaacacgagg
     5461 atgtcgagcg gcaccatgat gtgcggcggc gatgcgagga acattccgat caccgcgcaa
     5521 aagacgatca acgatacgac tcgcggcttg gtcagttcgt agaactggcg gaaacggtgc
     5581 gatgcggtgc tgagtgcttc ggtcgtcatg gtgaagtccg tagcgttcgt ttcaagattg
     5641 agtggacagc ctgcctgcga ggcgggcgtc cggcaaagcc gcagcgcgat gcgtgatgaa
     5701 gtttatcacc acgtaaatca cgagcagtat ggctgcgccg ccgttgtgcg ccgcggccag
     5761 cagcaagggc agcgaataga ccacgttcag cacgccgagc gtgaactgca ataccagcgc
     5821 gccgagcagc agcagcccga gcttgcgcgt ggcgttcaac gccgtcatcc gcaagacgag
     5881 gtagagcgta accgcggtga cgacaagcgc gcccaggcga tgcatccagt gaatcgccgt
     5941 caacgcctcg ttcgacaaca gctcgccttg cggcgtcata cccagttcgc gcagcatgtg
     6001 aaaggcattg gcgaaatcca tggtcggcat caactcgccg cgacagagcg gcagatccgg
     6061 acatgccagt gccgcgtagt tgctgctgac ccagccgccg agcgcaatct gcagcacgag
     6121 caggaggagc ccgacgagcg cgggtgtgcg caagttggcc gcctcgggcg gagcctgcag
     6181 attcatctgg cgcaacgcca gccagctcag catggcgaga atggtcatac cgcccagcag
     6241 atgcagcgtg acgatcaccg gcttgagcaa cagtgtgacc gtccacatgc ccagcgtggc
     6301 ctgaaccacc actacggcga gcaagacggt cggcagcgcc ggcgactgac gcaaggcggt
     6361 acgtttggtc caggcgatca ccgcgatcgt cagcagcatc agccccagaa agccggcgat
     6421 ataacgatga aacatttcct tccatgcctt ttgcatggag acaggcccgt gctcaccgcc
     6481 ctgctcggcg actgccagcg aaatctgatc gctcgcatgg aagggcgtga cgcggccgta
     6541 acaaccgggc cagtccggac accccagacc ggcatcggac aggcgcacat aggcaccgac
     6601 aaccaccacc acaaaagtca ggagcgccgc aagaaaaatg gttttgcgat acatgttcaa
     6661 cctacccgcg aaacctgcag cagacgctgc aggtctttga tgattcgatg cggctccgga
     6721 ttgggcggaa agcgcagcat gagatttccg agcggatcga tcagatagat atgctcgctg
     6781 aggtctcccg tcgcagggaa ttgcgcacga aaggcggacg ggacttcctg cccgatgtag
     6841 ctgccttcat aaagcgcaga cggcgccgca ggcgtctgac catcgagtac gaaccagacg
     6901 cgctcgacgc gatccatgtg cttgccctgc gccgtgcgcg attggcgaat gtgatagagc
     6961 ttgcgttcgc atgcttcgtc gcaacggctt tcgttgaatt gcaccatcag ccatttaccc
     7021 cgcaacgccg ctaacgaagt gcgttcgcct tcgggcgccg tcaatgcgac atcgggcagc
     7081 ggcgtgggcg acaacagctc gccgtagttg gtatgcgcgc tgggcggaaa gtagtagaac
     7141 gccagcgtgc cgagaacgaa gggcgccgcg cagatggcga atatcgtgag cagatagcgc
     7201 ctggccgtgc ggcgacgcgc ggcgtcctgt gccgggctgg aatcagtcac ggttgcgttt
     7261 gagattgagt gcgacataca gaatgagact cagcgtagca aaggaatacc attgcatggc
     7321 gtatatccgg tgtttttcga tgcccacatc cgggcgcggc caatcgcgca ccagcccgtc
     7381 ggcgacgtcg ttggtctgcc ggatgacgaa gggttgcagc ttgagcttcg accacgcggc
     7441 gtagcggtcc aacaagaggt tttgccatac atatcccggc tgcacttcag ccaactccag
     7501 cacgcggtcg ctgggaatcg cggcaatgcc ttccacctgc cgtacgcctt ccggcgtctg
     7561 gatgaccggc aactgatcgc gcgtcgcacc gccgacgacc cagccgcgca cgaccagcac
     7621 atgcatctca ctgtcttcca ggcgcagcgg cgtaacgacg tgataaccca cccgaccccg
     7681 atgcaccttg ttgtcgatca ggatcgtacg atcgacgacg tagcgtccgg ttgccacgac
     7741 aggccgcagt tcgatgtcct tcgaatcgac cggcgtcaca ggcacttgga gcggcggctg
     7801 cgcagcccaa gcgtcgagct tctgctgcag gctcgctttc tccagcgcac gactgcgctg
     7861 ccagtttccg gcggagatgg tgacggcgca aaagacgatt gtcagcgcgg tcatgaacca
     7921 gctcgggcgg aagcggtagc gtttggctaa ggacgacatt gcatggcttc tcggcggccg
     7981 ctcgactaaa cttgcacagc ctgttcgaat tctcctctat gacttatatc gtcctcctgg
     8041 ccttcgtgct catcatcggc tcactggttt cagcgctggt gttcatggtg cgtgaccgcg
     8101 gccgatcgaa gcggacggta tacgcacttg ccttgcgcgt gggtttgtcg ctgacgctgt
     8161 tcctgttgct catgctgagt tatcgcatgg gctggatcac cgggcggctg ctgcaataag
     8221 cgcaaaaaaa tgcgccgcat gacgcggcgc ccagtgcgct ccgacgggag cgcctcctcc
     8281 tcaatcggac gggcaccgca cgcccgtcag tcgcttacaa ccagtacacc acgacgaaca
     8341 gtccgagcca gaccacgtcg acgaagtgcc aataccacgc ggcagcctcg aatgcgaagt
     8401 gatgctccgg tccgaagtgt cccttgatgc tgcggatcag gatgacggtc agcatgatgg
     8461 caccgaccgt cacatggaaa ccgtgaaagc ccgtcagcat gaagaaggtc gaaccgaaaa
     8521 taccggaggt gagcttcagg ttcagatccg aatacgcgtg catgtactcg tagatctgga
     8581 agcccaggaa ggtcgcgccg agcgccacgg tcaggaacag gccgaggatc agttggctgc
     8641 ggttgttctc tttgagtgca tgatgcgccc aggtcaccgt cacgccggaa gtcaggagca
     8701 gcaacgtgtt cagcgccgga ataccccagg cgctcatggg cgtgaattgc gtatcgaagc
     8761 cgggtcctgc ggtcggccac tcggccccgt aatccggcca cagcagtttg gattcgatgc
     8821 cgcccagatc gggcaccgaa tgcgcacgcg cccagtagag cgcaccgaag aacgcgccga
     8881 agaacatcac ttccgaaaag atgaaccagc tcatccccca gcggaaggaa acgtccacgc
     8941 gcttgttgta catgccgcct tcggattcgt gaatcacgtt cccgaaccag ccgaacatca
     9001 tggagaccag cccgaggaac ccgatgagca acacccagcc gccgatgccg atgccgttga
     9061 accagatcgt cgcaccgagt gccatggaga acagcgacac cgaaccgacg atcggccagt
     9121 gcgacggctc cggtacgaag tacttcgcag tgttgctcag gaccatgtcc cctcccgctt
     9181 atttttcatt ccattcgtgg ccgtaaggcg cacgccaaag attccctgca aaccgatact
     9241 cgcgttcacg gcactcaacc tttcttcccc gccgtgccgg ccacctcgaa gaacgtgtag
     9301 gacaacgtaa tggtgttcac gtccttgggc agatccgcgt caagcaggaa cacgaccggc
     9361 atttcgcgca cctcgccggg accgagcgtc tgctgttcga aacagaagca ctccatcttc
     9421 ttgaaatgga gtccggccag ttgcggcccg aagctgggta cagcctgccc ggtgacgggg
     9481 cgatcctgct tgttgcggac ttcgtagctg atcgtcgtca gttcgccggg atgcaccgtg
     9541 acgaaacgct gcttgggctt gaagtcccag ggcagatggt gcgtgttcga gtcgaattcg
     9601 atggtgacgg tacgcgcgta gtcgatctgg gtattcaccg gctcggcgtc gcgcttgacg
     9661 atgctgcgaa gaccggtgac ctcgcatatc tgctcgtaga agggcacgag cagaaagcca
     9721 aagccgaaca tcatgccggc gacgacaagc agcttcttca tcaatcgctt gttcgcatcg
     9781 gcagcggact tggtcggtgt gctcatgatc ccagtagcca gtacttgaag atgatgccga
     9841 aaaagaacgt cgccacgata ctgagcagaa tcagcgccgt tttgacgttc ttcgacatat
     9901 gcggccggac ctcaatggac tgcgatcagc gtacgaccgg cggctcttcg aagctgtgat
     9961 acggcgcggg cgacggcaga tgcgtccact cgagcgtatc cgcaccttcc cacggacgat
    10021 cgggcgcctt ctctcctttc cgcatcgcgc attgcagcgc gacgtagagg aagatcaatt
    10081 gcgccaggcc gaagccgaac gcgccgatgg aagcgaccat gttgtagtcg gcgaactggg
    10141 gcgcgtagtc cggaattcgg cgcggcatac cggccattcc caggaagtgc atcgggaaga
    10201 aggtgatgtt gaagaaaatc atcgacagcc agaagtgcca cttgcctagc gtctcgttgt
    10261 acatccggcc ggtccatttg ggcagccaga agtaggcgcc ggcaaacatg gcgaacaacg
    10321 aaccggccac cagcacgtag tggaagtgcg caacgacgta gtaggtgtcc tggaattgga
    10381 tatccaccgg cgcaatggcg agaatcagcc ccgtgaagcc ccccaccatg aacacgaaga
    10441 tgaagccgat ggcaaacagc atgggcgtct cgaaggtcat cgagcccttc cacatcgtcg
    10501 ccacccagtt gaacaccttc acgcctgtcg gcaccgcgat cagaatggtc gcgtacatga
    10561 agaacagcag accggcggta ggcatgccca ccgtgaacat gtggtgcgcc cagacgatga
    10621 acgacaggat cgcgatcgaa gcggtcgcat acaccatcga gctgtaaccg aacagcggct
    10681 tgcgcgcgaa ggccgggacg acctgcgaga tgatgccgaa ggccggcaga atcatgatgt
    10741 agacctcggg atgcccgaag aaccagaaga tgtgctggta catcaccggg tcgccgccgc
    10801 ctgctgcatt gaagaacgag gtgccgaagt ggcgatcggt caggttcatg gtgatcgcgc
    10861 cggcgagcac gggcatcacg gcgatcagaa ggtatgcggt gatcagccaa gtccacacga
    10921 acatcggcat tttcatcagc gtcatgccgg gtgcgcgcat gttgagcacc gtcaccacga
    10981 tattgattgc gcccatgatc gaagaggcgc ccaggatgtg aacggcgaag atcgccagat
    11041 cctggccggg acccatttgc gtcgaaagcg gcgcatacat cgtccaaccg gcgcctgccg
    11101 caccgcccgg aacgaagaag gatgcgatca gcagcgtcgc ggccgcgggc agcaaccaga
    11161 agctccagtt gttcatccgc gcaaaggcca tgtcgggcgc accgatcatc agcggaatct
    11221 gccagttagc gaaacccacg aaggccggca tgatcgcgcc gaagatcatg atcaggccgt
    11281 gcatggtggt gagctggttg aagaactcgg ggttccacac ctgcatgccg ggcacgaaca
    11341 actcggctcg aatgccgaag gccagcaagc cgccgacaag gaacatggtg aaactgaacc
    11401 acagatacaa cgtgccgatg tccttgtggt tcgtggtggt gatccaccgc atgatgccgg
    11461 tcggatggtg accgtggtcg tgggcgtggc cgtgctgatc caggactgcg ctcatggttt
    11521 gctcctagct ttcttatttg cgggccgctt tgatttcggc cggctgcacc acattctcgg
    11581 caggcttgtt accccaggaa ttacgcgtat acgtaatgac tgccgccaga tcgacgtcgg
    11641 acaattgcgg gccgaaggcg gccatggcgg tgccgggttt gccattcatc acgagatcga
    11701 tatgcgggcc cttgggaccg ttcacgatgt ccgagccggc ctgtgccggg aacatcggcg
    11761 gcgtgccctg gccgttgccc tgatgacaga cggcgcatgt cgttccgtaa accttttcgc
    11821 cgcgagccat tagctcgtcc ttgttccatt ccttgctcag gtcttcggcc ggcgccgcgg
    11881 gcgcggcggc ggcaggggcg ctcgtttcgg ctgcggcaac ttggacgccg gccggctgta
    11941 ttccctgcgc ctgacgggtt ttcgctaccc agtcgctgta cgcgtcggcg gagacgaccc
    12001 gcacgacgat cggcatgaag gcgtggtcct tgccgcaaag ctctgcacac tgaccgcggt
    12061 aaacgccttc tttttcggcg tagaaccagg tgtcgcgcac gaatccggga acggcgtcct
    12121 gctttacgcc cagcgcgggc acccaccagg catggatcac gtcgttggcg gtggtgatga
    12181 tgcgcacttt cttgttgacc ggcaccacca gttcgcggtc cgtttccagg agatagtcgg
    12241 tacctcgctg cgcaccggca taaatctgtt catacggcgt gctgagattg gcgagaaagc
    12301 tgatgccttc gccctcgccc ttgatgtagt cgtagcccca cttccactgg tagccggtcg
    12361 ccttgatggt gatatctggc gcggacgtat ccttgatggc gagtaccgac ttggtcgccg
    12421 gccaggccat gccgaccagg ataaggaacg gcacgagcgt ccagatgatt tcgaccgtgg
    12481 tgttctcgtg gaactgttcc gccttcgcgc ctaccgcctt gcggtgcttg acgatcgaat
    12541 aaatcatcac cgcgagtacg ccgaagaaca ccaccagaca gatggccagc atcaggtagt
    12601 gcaggtcata gatttcgcgt gccaccggcg tgacaggtgg ttggaagtcg tgcctgttcg
    12661 gttgaatctg ctgcgcgcag acgactccag aaaacaacgc gagcgcaaag ccggcaaccg
    12721 gcagaagtcg cgacttgatg gtcatgtagt ccccgttgtg tgcaatcgta atcgttctaa
    12781 aacgctccct ggccgtcatt ccgctttgga accggccaac cttgcgcgca acaatcgcgc
    12841 caggcttttc ctcccgggca tgtccaaatg ccgcccgacc tccaactccc tgccgctctc
    12901 ggccaatgca atacgcaaac cgttgcgacc tgcatgtgcg cacacgcgta catgcccagc
    12961 gtggaagcga tggatcgata cacgctcgcc atcgccgact tctacgatca actctccgtc
    13021 tcgacgcacg atccgctcgt agtccgcggc atgtcggccg ttgacgtaga gcgccgccgc
    13081 aagtgccagc atctcgatcc cggcaaaagg aaacaccagc cacgcgcccc gtagggcgaa
    13141 gcctgtagcg attccgaagg acaagagcac cgtcgcgaga agcagccaca acagtgtctg
    13201 cggcgagatg gagcagttgc gcttcaccgt gaggctgaaa tcacccaccc ccacggatgc
    13261 cggaactttg atgcacgcat cgtccatgct tgaacagctt gtgacggcag cgaccgcacc
    13321 gcaatcgagg cgacttaact taacatagct cacagaaagg cggcaagttt tgtggcaacg
    13381 taaacgcacg cagaagcgcg tgcgctacgc cccgaaagcg ccacccgatg caaggtgcgc
    13441 ggctaagcgc aaaaacgcgt gaaaaaactc agcgcgacgc gcgtttttcg cgcggtgtga
    13501 aggtgatgat ttgccgtccg tccgacaacg cgcgcggggc ggccttccat gcgtggccgt
    13561 aaatgatttc gaactctgcc gcgatgcgtc cggctccggc cataccggct tcgtaagcac
    13621 gaatcatttt cgtccagcgc tcgcgccccg taagcgttcg ccgccgcatg cgatgcgcgt
    13681 tgcggctgcc cgtcccccgc aattcgcaca gcagcgcgtt cacgtccgga taggtgagcg
    13741 tcagcatttc catgtccacc accgggtcgg cgaacgggca ggcgaccaaa gcgtcgccca
    13801 ggtcgtgcat gtcgacaaag gggtgaacgt gcggcagcgc atcaaccgcg gcgaaggact
    13861 cgcgcagggt gcgcagcgta tccggtccgt aggtggtgaa catgagcagg ccgtcgacct
    13921 tcagtacgcg gtgccactcg cgcagccagg cgaccggatc gggcgcccag gcaagcgcga
    13981 ggttggacca cacaacatcg aagctgcgat cggcaaacgg cagggcaccg gcattgccgg
    14041 caagcaggaa gtcgcgccgc ggtgcaaaca gtgcgcgcgc ccggtcgacc agcgaacggg
    14101 aagcggcgtt gtgacgcagc gccggcatgg cccagtccac cgcatagacg tccgcggccg
    14161 gataacgctc gcgcaagcgg atccggctga ggcccgtgcc gcaaccgatg tcgagcaccc
    14221 gctcgggtgt gaccttgatg tattccagac gctcgaacag ccggcgctcg atttcgcgcg
    14281 ccagcacgtc ttccgcgctg gcgcgttcga agccgcgtcg cgccgctacg gaatcgagcc
    14341 aatcttcgat gaggcgcctc cgcggcgtca ggcgagcgcc gacagaccga gcttggtcag
    14401 gagcgcgcga tcggcgtcgg cttcgggatt gcccgtcgtc agcagcttgt cgccgtagaa
    14461 aatcgagttg gcgccggcgt gaaaacagaa agcctggacg gcttcgtgca tttcctgtcg
    14521 tccggcggac aaccgcacaa agctcttcgg catggtgatg cgcgccactg cgatcgtacg
    14581 cacgaactcc gtccagtcga gcgactgact ttcggccagc ggcgtgccgg gcaccggaac
    14641 gagattgttg atgggcacgg attcgggcgg tggcgtcatg ctggccaact gcgcgatcat
    14701 gccggcgcgc tgcgaacgcg tctcacccat gccgacaatc ccgccgcaac agacatgcag
    14761 gcctgcctcg cgcaccttgt ccagcgtcga caggcgatcc tcgtactggc gagtggaaat
    14821 cacttcgccg tagaactcgg gcgccgtgtc gatgttgtgg ttgtagtagt ccagccccgc
    14881 gtcgcgcagc tgttcagcct ggccgtcgcg caacaagccg agcgtggcgc aggtttccag
    14941 gccgagcgat ttcaccgcgc ggaccatttc cagtaccggc tcgaggtctt tctgcttcgg
    15001 tcctcgccag gccgcgccca tgcaaaagcg tgtcgcgccc ttcgctttcg ccgcggatgc
    15061 cgcggccagc acttcctcga cagacagcag tttctggctt tccacacccg tgtcgtagcg
    15121 tgcggcctgc ggacaatagc cgcaatcttc cgggcagccg cccgtcttga tcgacagcag
    15181 ggtcgacaac tggatcgcct gggggtcgaa attcgcgcga tgcacttcgt gcgcgcgatg
    15241 aatcaattcg ttgaacggca gttcgtacag cgcttccact gcggcgcgcg tccaacgagg
    15301 actttgcaca ggcgcctggg gaacaacgga agaggggggg cttgcggttt gaggcatact
    15361 tttctccggg gcggaagtgt caccgccgtg actgaaactg tcaaaccatg cttggtctgt
    15421 ctccggcgct gcggcggctt cgcagtgcac tgctcgcaca ggactgcgca ctttgcgccg
    15481 cacccagcgg cgacgcattg atttgtgcga cgtgtacggc ggaattgccc gccatgacgt
    15541 cggcctgtcc gcaatgcggg gatgccagcc cgggcgatca ggtctgcggc gcctgtctcg
    15601 cacatccgcc tgcctacgac gcgaccattg cggcctggcg ttacgatttt ccgctggacc
    15661 ggctggtgca ggcgttgaaa tacggcaacc gcctggcgtt ggccgcctgg ttcgccgaag
    15721 cgctcgccgc gcgcctcgac gaaaggcgcg tcgatctggt cgtgccgctg ccgcttcatg
    15781 cgcggcggct gcgcgagcgc ggcttcaatc aggcaatgga aatcgcccgc cgtctgcgaa
    15841 aacccgccgg tacaccgccc gcgccgaatg tcctggtccg gtcggcggat accgccgcac
    15901 agaccggatt gtcgaacgag gcgcgcaaac gtaatgtacg caatgccttc atcgcgacgc
    15961 agtcgctcga cggtctgcgt gtcgcgctcg tcgacgacgt gatgaccacc ggcgcgacgc
    16021 tcgacgccgc cgcacgcgct ttgaagaacg ccggcgccat ccacgtggag aactgggtgg
    16081 tcgcgcgcgc cgttcgcaac gactgagctc gattcatgct cgatatcgtg ctgtatgagc
    16141 cggaaatccc gccgaacacc ggcaatctca tccgtctggc ggctaatagc ggcagccggc
    16201 tgcatctggt gaagccgctc ggcttcagcc tgagcgagcg ggcgctcaaa cgctccggac
    16261 tggattacgg cgcgctcgcc tcggtgacgg tgcatgagga ctggtcggca tgccggtcgc
    16321 actttgccgg ccgtaactgc tacgccgtca cgacacgcgg ggtgcgctgc tacgcggacg
    16381 ttgcctatgc gcgcgacgac gtcgtggttt tcggccccga gacgcgcggg ctgccggccg
    16441 aggtgctggg gcaattcccg gacgagaatc gtctgcggtt gccgatggcg gcgggcaatc
    16501 gcagcctcaa tctggccaac gccgtatcga tcgtcgtcta tgaagcctgg cggcaggcgg
    16561 atttcgtcgg cggcgcctga atcagctttc cgccttggga tcgcgcgcaa gcaactgctc
    16621 cacggcctca cgcgccgaca cgccacggaa cagcacatcg cacacggcgc gggtaatcgg
    16681 catatccacg ccgagttccg ccgcgcggcg ttccacttcg cgcgcggtat tcacgccctc
    16741 ggcgacatgc ccgagccgct tgagaatggc aggcaaggtg tcgccccttc ccaattccag
    16801 gccgactttg cgattgcgcg acagatcgcc cgtacaggtg aggatcaaat cgcccaggcc
    16861 ggtgaggccc atgaaggttt cggtgcgccc gcccatggcg acgcccaacc ggctcaattc
    16921 ggccagcccg cgggtgatca gcgcggcacg cgcattctgg ccgagcgaaa ggccgtcgca
    16981 aacgccggtg gcgatggcca tcacgttctt gaccgcgccg cccacttcga cgccgggcaa
    17041 atcgtcgctg aaataggtgc gcagcagcgg ttgatgcagt tccgcggcgg ttcgacgcgc
    17101 gaacgcctca tcgtgcgcgg caagcgtgag cgcggtcggc tgcccgcgcg ccacctccat
    17161 ggcgaagctc gggccggaaa gcgcggcgca gcgcgcttcg gtgccgatca cctcgcccac
    17221 cacctgatgc ggcaacaggc ccgtatcgga ttcgaagccc ttgcacgccc acaccaccgg
    17281 tacctgctgg ttgacgtttt tgaccagccc cagcgtctca cgcaggccgg cggtcggagt
    17341 ggcgatcaac accaggtcgg cgccctgcaa agcagccggc agatcgtcga cgacgtcgat
    17401 cgcgtcgggc agcgttgctt cgggcagata aacgctctgc cgttcgcggc gcagcgtggc
    17461 gcgctgctcg tcgttgcgcg tccacagacc gatacggtgt tgtcgttc
//
LOCUS       DATGOU010000093         4672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_1150474, whole genome shotgun sequence.
ACCESSION   DATGOU010000093 DATGOU010000000
VERSION     DATGOU010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150474"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            459..1253
                     /gene="fabI"
                     /locus_tag="VKZ48_05670"
     CDS             459..1253
                     /gene="fabI"
                     /locus_tag="VKZ48_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HLU77375.1"
                     /translation="MGFLADKRILVTGLLSNRSIAYGIAQACHREGAQLAFTYQTDRF
                     KDRVAEMAAAFGSDIVLPCDVSDDAQIDALYAALGQRWDRLDGMVHAIAFAPREAIAG
                     DFLEGVSREGFRIAHDISSYSFAALAKGALPMMTEGGALLTLSYLGAERVVPHYNTMG
                     LAKASLEACVRYLAESLGPKGIRANGISAGPIKTLAAAGIADFGKIFKFVERNAPLRR
                     NVTIEDVGNVAAFMLSDLAAGITGEITHVDSGFNIVTGMGELEAKD"
     gene            complement(1310..3193)
                     /locus_tag="VKZ48_05675"
     CDS             complement(1310..3193)
                     /locus_tag="VKZ48_05675"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HLU77376.1"
                     /translation="MFDFVGKNKRLLQIFLAIIIVPTFAFFGIQSYEGFFGSGADVAE
                     VAGEPITVREFERALEQQRNRLREAFGGNIDPAMFDSPEARRELLDSLVNQRVLLHYA
                     FTRRMLAGDEAIREFIAAHPAFQDDNGFSRERYEALLRAQNMSPAGFEAQLRTDIALE
                     QLTEAIGQSAFVSRAVAQRFARERAETREVSRTLMNAGEYAGKVSVTPEEIDAYYAEN
                     QQEFETPEQVRAAYVVLDRDAFASRIEVNEEEVRKQYEARIAPQMEAREQARAKAQSV
                     LAELRKDPSRFAELAKQHSDDPGSAEQGGDLGFFGRGAMVKPFEDAVFKLKAGEISNL
                     VETEFGFHIIRLNEVKGGERHASHILFNAPSVADDPDAARKQIADEMRRQQLARQFPE
                     AAEAYANLAYEQPDTLEPLVDRFGLKVAESGWLSRTAGRPPLDNPRLLDALFGADAIE
                     AKQNTEAVEVAPGKIVVARVIEHKARAMRPLADVRDDIRKTLLERKALKMAQQAGQEA
                     LDALRRGDQVRANWNAPQRVSRENPGAMDVQTLTAIFKADVATLPAFVGVDTPGGYAI
                     YRISAVDTPKEVAEPRERAAAMALAQLNAQQELKAFVDALRERADVEVNEEQLLQAGS
                     RGR"
     gene            complement(3334..3410)
                     /locus_tag="VKZ48_05680"
     tRNA            complement(3334..3410)
                     /locus_tag="VKZ48_05680"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3374..3376),aa:Asp,seq:gtc)
     gene            complement(3422..3497)
                     /locus_tag="VKZ48_05685"
     tRNA            complement(3422..3497)
                     /locus_tag="VKZ48_05685"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3462..3464),aa:Val,seq:tac)
     gene            complement(3542..3814)
                     /locus_tag="VKZ48_05690"
     CDS             complement(3542..3814)
                     /locus_tag="VKZ48_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HLU77377.1"
                     /translation="MNKTEMIDYIAEKADISKAAAQRALDATVDSIQQALAKGGMVTL
                     VGFGTFYVGDRAARTGRDPRTGNTIEIKAAKVPKFRAGKALKDALN"
     gene            complement(3969..>4672)
                     /locus_tag="VKZ48_05695"
     CDS             complement(3969..>4672)
                     /locus_tag="VKZ48_05695"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008113665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HLU77378.1"
                     /translation="TVTSRNLDKFQGVRRYTYGMAEKENQVGQVTGLAWTEVGGELLT
                     VEVAVMPGKGKTMTTGKLGDVMQESVQAALSVVRSRAPRLGIPEDTFQKKDLHVHLPE
                     GAVPKDGPSAGISICTAMVSALTGIPVRADVAMTGEITLRGEVLVIGGLKEKLLAAHR
                     GGIKTVLIPEENVKDLVDIPDNVKNKLEIIPVKWIDKVLEVALERVPAPLAAPESGTV
                     PPATEDKATPTVITH"
BASE COUNT          782 a         1573 c         1416 g          901 t
ORIGIN      
        1 ccgcgctcgc tcgccggata ggggtcgttc cacacctcac cgcaaccggc cagcagcagc
       61 gcgaacagcc atgcgatgca gccggcgtag cggacggcga tcggtcgcac gggaattggg
      121 atattcaagc cggtagagtt ctgcgacacg aagcgtcgtg ggtcgaaacg ggcgctgtgc
      181 ggaccggtcg agtgtagcct accccctatg gcgccgtcat ccgcctgttc cgtcgcgcac
      241 gcgcctcgca gcgtgcacac cgaacccctc aaaggacggg aaagctgctc gagcagtcgt
      301 tataatcccg caagctttct ccccctggat cgacatgggt tttagttccg acgtcacgcg
      361 ccctgcgggc gcatgagtct ccactgaaac cctaaagggc acggaaagcc cctcaagcag
      421 tcgttataat cgcgcaatct ttctccccct ggatcgacat gggttttctc gcagacaaac
      481 gcatcctcgt caccggactg ttgagcaacc gctcgattgc ctacggcatc gcgcaagcct
      541 gtcaccggga aggggcgcaa ctcgcattca cctaccagac cgaccggttc aaggaccgcg
      601 tcgcggaaat ggccgccgcc ttcggttcgg acatcgtcct gccctgcgac gtgtccgatg
      661 acgcgcagat cgacgcgctt tacgcggcgc tgggacagcg atgggaccgg ctggacggca
      721 tggtgcacgc catcgccttc gcgccgcgcg aagccatcgc cggtgatttc ctggaaggcg
      781 tatcgcgcga gggtttccgc atcgcccacg acatttccag ctacagtttc gctgcactgg
      841 ccaagggcgc gctgccgatg atgaccgagg gcggtgcgct gctcaccctg agctatctgg
      901 gcgccgagcg cgtcgtgccg cactacaaca ccatgggtct ggccaaggcc agcctggagg
      961 cctgcgtgcg ctatctggcc gaaagcctgg gccccaaggg catccgtgcc aacggcatct
     1021 ccgccggccc gatcaagacg ctcgccgcag ccggcatcgc ggacttcggc aaaatcttca
     1081 aattcgtcga gcgcaacgcg ccattgcgcc gtaacgtgac catcgaagac gtcggcaacg
     1141 tcgccgcctt catgctgtcc gatctggcgg ccggcatcac cggcgaaatc acccatgtgg
     1201 acagcggctt caacatcgtc accggcatgg gcgaactcga agccaaagac tgaaacgcga
     1261 aggccgcccg caggcggcct tccccttccg atgcggcagg tcgtcgcgct cagcggccgc
     1321 ggctgcccgc ctggagcaac tgctcctcgt tcacttccac atccgcgcgc tcgcgcagcg
     1381 catcgacgaa ggccttcaac tcctgttgcg cattgagctg cgccagcgcc atggcggctg
     1441 cacgctcgcg cggctcggcc acttccttcg gcgtatccac cgcgctgatg cggtagatgg
     1501 catagccccc gggtgtgtcc acaccgacga aagccggcag cgtggcgaca tccgccttga
     1561 agatcgcggt caacgtctgc acatccatcg cgccgggatt ttcccgactg acccgctgcg
     1621 gcgcattcca gttggcgcgc acctggtcac cccggcgcaa cgcatcgagc gcctcctgcc
     1681 cggcctgttg cgccattttc agcgccttgc gttcgagcag cgtcttacgg atatcgtcgc
     1741 ggacatcggc cagcggccgc atggcgcgcg ccttgtgctc gatgacccgg gccacgacga
     1801 tcttgccggg tgccacctcc accgcttcgg tgttctgctt ggcctcgatc gcgtcggcgc
     1861 cgaacagcgc atcgagcagg cgcggattgt ccagcggcgg ccggccggcg gtgcggctca
     1921 accagccgct ttccgccacc ttgagcccga accggtcgac caatggttcg agcgtatccg
     1981 gctgttcata agccaggttg gcataggctt cggccgcctc ggggaactga cgcgccaact
     2041 gctgacggcg catctcgtcg gcaatctgct tgcgtgcggc gtcaggatcg tccgccacgg
     2101 acggcgcatt gaacaggatg tggcttgcgt gccgttcccc gcccttcact tcattgagtc
     2161 gaatgatgtg aaagccgaac tccgtctcca ccagattgga gatctcgccg gccttcagct
     2221 tgaacacagc gtcttcgaac ggcttcacca tggcgccgcg cccgaagaag ccgaggtcgc
     2281 ctccctgctc tgccgaaccc ggatcgtcgg aatgctgttt ggccagttcg gcgaagcggc
     2341 tcggatcctt gcgtaattcc gccagtaccg attgcgcctt tgcacgtgcc tgctcgcgcg
     2401 cttccatttg cggtgcgatc cgtgcctcgt attgcttccg gacttcctcc tcgttcactt
     2461 cgatgcgtga ggcgaaggcg tcacgatcaa gcacgacata ggcggcgcgc acctgttcgg
     2521 gcgtctcgaa ttcctgctga ttctcggcgt agtaggcgtc gatctcctcc ggcgtcacgc
     2581 tgactttgcc ggcgtactcg cccgcgttca tcagcgtgcg ggagacttcg cgcgtttcgg
     2641 cgcgctcgcg cgcgaagcgc tgcgccacgg cgcgggatac gaaggcgctc tgcccaatcg
     2701 cttcggtcag ttgctccagc gcgatgtccg tgcgcaactg cgcctcgaag ccggccgggc
     2761 tcatgttctg tgcgcgcagc agcgcttcat agcgctcgcg ggaaaatccg ttgtcgtcct
     2821 ggaaggccgg atgagcagcg atgaattcgc ggatcgcttc gtctccggcc agcatgcggc
     2881 gggtgaaggc gtaatgcagc agcacgcgct ggttcaccag cgaatcgagc agttcgcggc
     2941 gcgcttcggg cgaatcgaac atggccggat cgatgttccc gccgaaagcc tcgcgcaggc
     3001 ggttgcgctg ctgctccagc gcacgctcga actcgcggac ggtaatcggt tcgccggcga
     3061 cttcggcgac gtcggcgccg gagccgaaga aaccctcata ggactggatg ccaaagaaag
     3121 cgaaggtcgg cacgatgatg atggccagga aaatctgcag taggcgtttg ttcttgccaa
     3181 cgaagtcgaa catgagctgt cccggtatgg ctcccgcggg cgtcgcgcgc tttgcgtccc
     3241 cgcaaagcgt cccgctcggg caataaaaaa ggcgaaccga cggttcgcct tgctttacga
     3301 ctgtgaagcg aactgcgggc cgcttctgct gtgtggcgga gtggacggga ctcgaacccg
     3361 cgacccccgg cgtgacaggc cggtattcta accaactgaa ctaccactcc ccttctgcaa
     3421 ctggtgggtg ctgacgggat cgaaccgccg acattcgcct tgtaagggcg acgctctacc
     3481 agctgagcta agcacccctt cgcgctgcgg atcttgcacc gatcccgcgc tcagagcgcc
     3541 gctagtttaa tgcatccttc agcgctttgc cagcacggaa tttcggcact ttggcggcct
     3601 tgatttcgat ggtgttgccg gtccgcggat cacggccggt gcgtgcggcg cggtcgccta
     3661 catagaaggt accgaaaccg accagggtca ccataccgcc cttggcaagc gcctgctgaa
     3721 tgctgtcgac cgtggcgtcg agcgcacgct gggcagcggc cttggagatg tccgccttct
     3781 ccgcgatgta atcgatcatt tcggttttat tcatattcac tgtgtctcct tccatgagat
     3841 ttgaattggc ggatgcgacg cgttgacgcc gtacccacgc cgcatccgct gctttgtcgg
     3901 ccctgcatcg gtctgtcggc aagacaatgc cggtcagcct cgcggccgcc ggcatcggac
     3961 attcactgct aatgcgtaat gacggtcggt gtcgccttgt cttcggttgc cggcggcacg
     4021 gtacccgatt ccggcgccgc cagcggcgcc ggcacgcgct caagtgcgac ttccagcact
     4081 ttgtcgatcc acttcacggg aatgatctcc agcttgttct tgacgttgtc cggaatgtcg
     4141 acaaggtctt tcacgttttc ttccgggatc aacaccgtct tgatcccacc gcgatgcgca
     4201 gccagcaatt tctccttgag gccgccgatc accaatactt cgccgcgcag cgtgatctcg
     4261 cccgtcatgg cgacatcggc ccgcaccggg atgcccgtga gcgccgaaac catggccgtg
     4321 cagatgctga tccccgcgct gggaccgtcc ttcggcaccg cgccttcggg caggtggacg
     4381 tgcaggtcct tcttctggaa ggtgtcctcc ggaataccca ggcgcggcgc acggctgcgc
     4441 accaccgaca gggcggcctg caccgattcc tgcatcacgt cgccgagctt gccggtggtc
     4501 atggtcttgc ccttgccggg catgacggcc acttccacgg taagcagctc gccgcccacc
     4561 tcggtccacg ccaggccggt gacctggccc acctggttct ccttctcggc catgccgtag
     4621 gtgtaacggc gcacgccctg gaacttgtcg agattgcgcg aggtgacggt ga
//
LOCUS       DATGOU010000094        17554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_1604662, whole genome shotgun sequence.
ACCESSION   DATGOU010000094 DATGOU010000000
VERSION     DATGOU010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17554
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604662"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VKZ48_05700"
     CDS             <1..199
                     /locus_tag="VKZ48_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77379.1"
                     /translation="FAGFEFLQGVEGVVGHGGSKSKGLNHQDTAAQRKKDKNLEPPRH
                     QDTKFHTTVLGVLLVPWCLGG"
     gene            complement(253..1236)
                     /locus_tag="VKZ48_05705"
     CDS             complement(253..1236)
                     /locus_tag="VKZ48_05705"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006028603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HLU77380.1"
                     /translation="MAKPPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLEV
                     TPALNALKGVAMELDDCAFPLLAGIVQTDDPKVAFKDADYALLVGAKPRTKGMERADL
                     LEANAQIFTVQGKALNEAAKRSVKVLVVGNPANTNAYIAMKSAPDLPRTCFSAMLRLD
                     HNRALSQLATKTGKPVGTMHKMIVWGNHSPSMYPDYRFATIDGQPAPAAVNDDAWYRD
                     TFIPTVAKRGAAIIEARGSSSAASAASAAIDHMRDWALGSNGQWVTMGVPSDGSYGIP
                     EGIVYGFPVVCANGGYEIVRDLEIDDFSRQKMDATLKELLEERDGIKHLLA"
     gene            complement(1206..1568)
                     /locus_tag="VKZ48_05710"
     CDS             complement(1206..1568)
                     /locus_tag="VKZ48_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77381.1"
                     /translation="MSRVTGLFFTFDAARPATLPRSQAALNRDASPLPDAGDYNKPFR
                     VFGGEDGTAMHARKQAGGLAPRRVDAPGARAYTARSLARASRASTIIFLRDCFLACIT
                     DEIILGVSPWQSLLYVSP"
     gene            1646..2026
                     /gene="sdhC"
                     /locus_tag="VKZ48_05715"
     CDS             1646..2026
                     /gene="sdhC"
                     /locus_tag="VKZ48_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HLU77382.1"
                     /translation="MKKKRPPLWLNISLFNLPVPGRVSILHRISGAALFLALPWLLFL
                     LDNSLASPERYARVQQHLNHPLAQLAVLGLLWAYAHHFCAGIRHLLLDLHVGIDLQSA
                     RASARAVLGASLALTLLFGWVLLW"
     gene            2020..2376
                     /gene="sdhD"
                     /locus_tag="VKZ48_05720"
     CDS             2020..2376
                     /gene="sdhD"
                     /locus_tag="VKZ48_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HLU77383.1"
                     /translation="MVTRAKRRVVGAHYGTAQWLRQRLTAVIMAVYTVLAIVALLILH
                     PENHAEWRALFAPGWVRVVTLLFFLSLCYHAWVGMRDILMDYARPTSLRLALYTAVVV
                     ALGGYAAWAVQIMWRG"
     gene            2378..4147
                     /gene="sdhA"
                     /locus_tag="VKZ48_05725"
     CDS             2378..4147
                     /gene="sdhA"
                     /locus_tag="VKZ48_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HLU77384.1"
                     /translation="MTKLVVRQFDAVVVGAGGAGLRAALQLSEAGLRVAVLSKVFPTR
                     SHTVAAQGGVGAALGNMGPDSWQWHMYDTIKGSDWLGDQDAIEFMCRTAPEVVIELEH
                     FGMPFDRNEDGTIYQRPFGGHMQNFGAGPVVQRSCCAADRTGHAMLHTLYQRNVRAQT
                     QFFVEWMALDLIRDAEGHVLGVTALEMETGEVSILQARATLFATGGAGRIFAATTNAF
                     INTGDGLGMAARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGYLLNCNGERFME
                     RYAPTAKDLASRDVVSRAMATEIKEGRGCGPKGDHVLLKLDHLGPEVIEKRLPGIREI
                     ARQFANVDPVRDPIPVVPTVHYQMGGIPTNYRGEVVVPGADGPSTVVPGFYAAGECSC
                     ASIHGANRLGTNSLTDLLVFGKSAGDSIIEYLRANPGHKSLPAEAGELTQARLARLMR
                     PEGGENVYEVMDDLRRVMQAHCGVFRFPDLLTEGVEMVRELSERVARTAVRDRSRVFN
                     TALVEALELDNLIEVARATMVSAEARRETRGAHDREDHHHRDDINWLKHTLWYKEGDR
                     LEYKPVNLKPLTATSFPPKARVY"
     gene            4172..4867
                     /locus_tag="VKZ48_05730"
     CDS             4172..4867
                     /locus_tag="VKZ48_05730"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HLU77385.1"
                     /translation="MHLRIYRYDPDKDAKPYMQDFHVELEPTDRMLLDVLVKAHEQDD
                     SLAFRRSCREGICGSDAMNINGKNMLACITKIDQVKEPVVIRPLSGLPVIRDLIVDMT
                     TFFQQYESVSPFLQNDEARPSRERLQSPEERERLNGLYECILCACCTSSCPSFWWNPD
                     KFVGPAGLLQAYRFIADSRDQALNERLDNLEDPYRLFRCRTIMNCTDVCPKGLNPAEA
                     IGRIRELLARRSL"
     gene            4867..5112
                     /locus_tag="VKZ48_05735"
     CDS             4867..5112
                     /locus_tag="VKZ48_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HLU77386.1"
                     /translation="MAMDEVVLRRLRWRCRRGLLENDLMLGRFLDRHADRLDERRVTQ
                     LQALLEFEDHDLWDVLAGRAECSDPALQDMVELIRAG"
     gene            5252..6547
                     /gene="gltA"
                     /locus_tag="VKZ48_05740"
     CDS             5252..6547
                     /gene="gltA"
                     /locus_tag="VKZ48_05740"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004525179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HLU77387.1"
                     /translation="MQPNGKAVLQYGEGKSVELPVLGGTIGPDVVDIRTLYGKTDMFT
                     YDPGFLSTASCRSSITYIDGEQGVLLYRGYPIEQLAQHCDFLEVCYLLIYGELPNQEQ
                     KDAFTAKVARHSLVHEQLARFYTGFRRDSHPMAVLVGTVGALSAFYHDSLDISIPEHR
                     EISALRLIAKMPTITAMAYKYSMGHPFVHPRNEYSYTANFMRMMFSVPNEDYQVNPVL
                     VRALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEACLQ
                     MLEEIGDVSRVGEYVKKAKDKDSGFRLMGFGHRVYKNYDPRAKLMRETCHEVLSELGL
                     QNDRLFKLAMELEKIALEDDYFVERKLYPNVDFYSGIVQRALGIPTQMFTCIFALART
                     VGWVAQWMEMIADPDQKIGRPRQLYTGATRRDVVPMDKR"
     gene            6745..9579
                     /locus_tag="VKZ48_05745"
     CDS             6745..9579
                     /locus_tag="VKZ48_05745"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HLU77388.1"
                     /translation="MLKQFLENSHLFGGNAPFIEDLYESYLDNPDSVSPEWREYFDRM
                     QLLPDQSGGEARDVAHAPVVHAFAQLARQRSLRTGPPTTELSVERKQIYVLWLIHAYR
                     FLGSRWAQLDPLKRLPRPHIPELEPAYYDLTEADLETEFNTGTLIGPERMTLREILQA
                     LRETYCGTIGAEYMYMSDPTQKRWIQQRLESMRSKPNFGADVRTRILERLTAAETLEK
                     HLHTRYVGQKRFSLEGGETLIPVMDELVRRAGAYGIEEVVIGMAHRGRLNVLVNTLGK
                     SPKDLFSEFEGKAVSELSSGDVKYHNGFSSDVATPGGPVHLSLAFNPSHLEIVNPVVE
                     GSVRARQHRRKDHSGDKVLSVLLHGDAAFAGQGVVMETLNLAQTRGYGTGGTVHIIIN
                     NQIGFTTSDPRDARSTIYCTDVAKMIEAPIFHVNADDPEAAVFVMQLAMDFRKEFKKD
                     VVVDLICFRKLGHNEQDEPFVTQPLMYKKIAEHPGTRQLYADRLVAQDVISPEEPEEL
                     IRQCRQALDEGRHTSSPVLSNYQRKYAVDWTPFLNTKWTEVADTTVPIAELERLAERI
                     TTVPEDFTLHPTVQRVIDSRRAMGRGEQPLDWGMGETLAYASLLVAGYGVRISGQDSG
                     RGTFAHRHAVLHDQDRERWDAGTYVPLSNLQEGQADFVVIDSVLSEEAVLGFEYGYAC
                     AEPNELVVWEAQFGDFANGAQVVIDQFITSGEAKWGRMCGLVMFLPHGYEGQGPEHSS
                     ARLERYLQLCAEHNIQVCVPSTPAQIFHLLRRQMLRPFRKPLVVMTPKSLLRRKEASS
                     QLAELAEGCFQTVISEVEDLDPQQVTRIIATSGRIYYDLAAARRERGADHVAIIRVEQ
                     LYPFPHEHFAAEMRKYPKAKEVVWCQEEPQNQGAWYQTQHYFLENIREDQKLYYAGRM
                     SSASPAVGYLAKHNEQQKNLLDMAFGPFKN"
     gene            9816..11102
                     /gene="odhB"
                     /locus_tag="VKZ48_05750"
     CDS             9816..11102
                     /gene="odhB"
                     /locus_tag="VKZ48_05750"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015601426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HLU77389.1"
                     /translation="MMLVEVKVPQLSESVSEATLLTWHKKEGQAVKRDENLIDIETDK
                     IVMELPAPADGVLTKIVKQDGDVVTSGEVIATIDTEAKPTQSESATAKAEAPVQASAQ
                     PQPALHPEPALVEPTPAAAPAEARQDARLMPAARKLVEEHNVDPSRIAGSGRDGRITK
                     ADVLDHLAKAPQPAQAEAKPSLPPAPPVNLDQVLAGRPEQRVPMSRLRARVAERLVQS
                     QRTAAILTTFNEVNMKPVIDLRKKYGERFQGEHGVKLGFMSFFVKAAVHALKKYPIVN
                     ASVDGNDIIYHGYFDIGIAVSSPRGLVVPILRDCDQLSVAEIEKKIADYGQRAQEGKL
                     SLEELAGGTFSISNGGVFGSMLSTPIINPPQSAILGIHATRDRPVVEDGQIVVRPINY
                     LALSYDHRIIDGREAVLALMAIKEALEEPARLLLDL"
     gene            11207..12628
                     /gene="lpdA"
                     /locus_tag="VKZ48_05755"
     CDS             11207..12628
                     /gene="lpdA"
                     /locus_tag="VKZ48_05755"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HLU77390.1"
                     /translation="MSDQSFDVVVIGSGPAGYICAIRAAQLGFKTACVEAWRNPAGKP
                     VLGGTCLNVGCIPSKALLQSSEEYDHVNKGLALHGIEVSGASIDVPKMIARKDAIVSK
                     MTGGIEFLFRKNKVSWLQGHGRLVKHSEQGWQLDVAGKAVQAKHVVIATGSKPRTLPG
                     VEIDNELVCDNVGALDFKTVPQKLGVIGAGVIGLELGSVWRRLGAAVTVLEALPEFLP
                     AADESVSREAWKIFTKEQGLDIRLGVKLGEVRRGKNNVSVVCEHDGRRETLEFDKLIV
                     AIGRVPNTDGLGAEGVGLQCDARGFIEVDSHCATSLPNLYAIGDVVRGPMLAHKGQEE
                     GVMVAERIAGQKPLVDLETIPWVIYTAPEIAWVGQTEQQLKAEGIPYRAGQFPFSVNG
                     RALCAGDPRGFVKMLAHRKTDEILGVHIINTHASELIAEAVVAMEFHSTAEDIARIVH
                     AHPSLTEALHEAALAVDKRALNI"
     gene            12726..13937
                     /gene="zapE"
                     /locus_tag="VKZ48_05760"
     CDS             12726..13937
                     /gene="zapE"
                     /locus_tag="VKZ48_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HLU77391.1"
                     /translation="MHDRQIEVESYQPLPPIGDGSVFDAAEYTKPAVRLPLTVLEYFH
                     DTLARRGYVPDESQYAAVQRLQRMYEQWMDYDARSKNLWKRFLLQPTPPRGVYLWGGV
                     GRGKSLLMDTFYLTVPEERKRRVHFHHFMRDVHREMETLKGKEDPLAAVASRIARRYR
                     LICFDEFHVDDIADAMILGRLLEQTMDRGVVYCMTSNYHPDALYRHGLQRERFLPTIA
                     LIKERLDVVHVDGGVDYRRRALQGVEIYHCPLGPEADAALWRAFEQIAEVEEEHHELD
                     VEGRVIPFEHRAGGVVWFDFNVLCGGARSHNDYLDLAKRFHTVIVSNIPQLTEDDADK
                     ARRFTWLVDIFYDRKVKLVVSAAVPPEQLYRDGPFVNEFARTASRLIEMQSNEYLAAS
                     RRDPQAAAIAA"
     gene            13957..14358
                     /locus_tag="VKZ48_05765"
     CDS             13957..14358
                     /locus_tag="VKZ48_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HLU77392.1"
                     /translation="MRQRACLTIEDCKRIAAAAEAEALKNKWEVAVAIVDDGGHLLHF
                     QRMDEATPANAEIALGKARTAAMSRRPSAMWEERIKNGRLSMLGMPLLPVQGGLPIIV
                     DGVCIGGVGVSGVQSHEDEQIAQAGIDALTA"
     gene            14455..15843
                     /gene="fumC"
                     /locus_tag="VKZ48_05770"
     CDS             14455..15843
                     /gene="fumC"
                     /locus_tag="VKZ48_05770"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004532308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HLU77393.1"
                     /translation="MAHRIERDTFGDIEVPAERLWGAQTQRSLQNFRISGERMPAELI
                     RALALVKRACAAVNKELGQLDDAKADAIVRAADEVLAGDHPDEFPLVVWQTGSGTQSN
                     MNMNEVLANRASELLGGERGEGRKVHPNDDVNRGQSSNDVFPTAMHVAAVEQIERRLL
                     PAIERLRDSLREKSAAFADIVKIGRTHLQDATPLTLGQEFSGYVAQLDHGLRHVRDAL
                     PHLSELAQGGTAVGTGLNAHPEFGERVAAELSRLTGLSFVSAPNKFEALAAHDALVHG
                     HGALKTLAASLTKIANDVRWLASGPRSGLGEITIPENEPGSSIMPGKVNPTQSEALTM
                     ACAQVFGNDVAVNFGGAQGNFELNVYKPLMIHAFLQSVRLLADGAESFDAHCVRGIEA
                     NRERIDELLHRSLMLVTALNPHIGYDKAAQIAKKAHRDGSTLKEAAVALGFVSEADFD
                     KWVKAEEMVGRR"
     gene            16000..16479
                     /locus_tag="VKZ48_05775"
     CDS             16000..16479
                     /locus_tag="VKZ48_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HLU77394.1"
                     /translation="MSKRSVAKKAALEKLYVVRWSKIHGRGVFAATDIPKGTRIVEYK
                     GKRISYKLACRLYPDVDGKPTHTFLFEIDDDTVIDAAQGGNAARWINHSCEPNCEAVE
                     EDGRIYIESTRKIKAGEELGYNYGITLDEPHTKKEKARWPCYCGKKSCRGTLLQPKR"
     gene            16602..>17554
                     /locus_tag="VKZ48_05780"
     CDS             16602..>17554
                     /locus_tag="VKZ48_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010928984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLU77395.1"
                     /translation="MSVTIQSVLNEERLFPPPADFVKQANISGMAAYEALCQEAERDY
                     EGFWARLAREHLLWQKPFTTVLDQSNAPFFKWFDDGQLNASYNCLDRHLDTQPDKTAI
                     IFETDDGQVSRITYRELHRRVCRFANTLKGHGAKMGDRILIYMPMSIEVVVAMQACAR
                     IGAIHSVVFGGFSAKSVHERIVDVGASLIITADGQMRGGREIPLKPAVDEAIAMGGCE
                     SVRNIIVYRRTGTAVAMQAPRDVWWHDVIEGQPEVCEPTWVNAEHPLYILYTSGSTGK
                     PKGVQHSTAGFLLWAKLTMDWTFDLQPEDVFWCTADIGWVTG"
BASE COUNT         3262 a         5423 c         5776 g         3093 t
ORIGIN      
        1 ctttgccggg tttgaattct tgcagggtgt tgaaggcgtt gtgggacatg gcggctcaaa
       61 aagtaaaggg cttaaccacc aagacacagc agcacagaga aagaaagaca aaaaccttga
      121 accaccaagg caccaagaca ccaagtttca cacgacggtt cttggtgttc tcttggtgcc
      181 ttggtgtctt ggtggttagt acactttttc tctgtgtctc cgtgtcgctg cggttcgggg
      241 ttgaagggtt gttcaggcga gcaaatgctt gatgccgtcg cgctcttcca gcagttcctt
      301 cagcgtcgcg tccattttct ggcggctgaa gtcgtcgatc tccagatcgc gcacgatctc
      361 gtagccgccg ttggcgcaga cgacggggaa gccgtacacg atgccctcgg ggatgccgta
      421 ggagccgtcg ctgggcacgc ccatggtgac ccattggccg ttgctgccga gtgcccagtc
      481 gcgcatatgg tcgatggcgg cgctggctgc gctggcggcg gaagaagagc cgcgcgcttc
      541 gatgatggcc gcgccgcgct tggcgacggt gggaatgaag gtgtcgcgat accaggcgtc
      601 gtcgttcacc gcggcggggg cgggctggcc gtcgatggtc gcgaaacggt agtcgggata
      661 catgctgggg gagtggttgc cccacacgat catcttgtgc atcgtgccga ccggcttgcc
      721 ggtcttggtc gccagttgcg acagcgcacg attgtggtcg aggcgcagca tggcgctgaa
      781 gcaggtgcgc ggcagatcgg gtgcgctctt catggcgatg taggcattgg tattcgccgg
      841 attgccgacc accagcacct tgacgctgcg cttggcggct tcgttgagcg ccttgccctg
      901 cacggtgaag atctgcgcat tggcttccag cagatcggca cgctccatgc ctttggttcg
      961 cggcttggcg ccgaccaaga gcgcgtagtc ggcgtccttg aacgcgacct tgggatcgtc
     1021 cgtctgcacg atgccggcca gcagcggaaa ggcgcagtcg tccagttcca tcgccacgcc
     1081 cttgagcgca ttcagcgccg gcgtcacttc cagcagttgc agaatgaccg gctgatcctt
     1141 gcccagcatt tcgccgctgg cgatgcggaa cagcaggcta taaccgatct ggccggcggc
     1201 gccggtcacg gcgacacgta caggaggctt tgccatggag aaactcccag aatgatttcg
     1261 tcggtgatgc acgctaaaaa acagtcgcgc aggaagatga ttgtgctcgc gcgggacgcg
     1321 cgggcgaggc tgcgggcagt gtaggcgcgc gcgcccggag cgtcaacgcg acgcggggcg
     1381 aggccgcccg cctgcttgcg tgcatgcatg gcggtgccgt cttcgccgcc gaaaacacgg
     1441 aacggcttgt tataatcgcc ggcgtccggc agcggggagg catcgcggtt tagggcagcc
     1501 tgagagcgag gtagggtcgc aggtcgcgcc gcgtcaaacg tgaagaacaa tccggtgacg
     1561 cgggacatgc atggtcggcg gggcgtgcag cgcctccttg gacgctatcc aacaagggag
     1621 tgatgcttag atgcctgaag ctgcggtgaa aaaaaagaga cccccgctct ggctgaatat
     1681 cagtctcttt aatctgcctg ttcccggccg tgtatccatc ctgcatcgta tcagcggggc
     1741 ggcgctgttt cttgcgctgc catggctgct gtttctgctg gataacagcc tggcgagtcc
     1801 cgaacgctac gcgcgcgtgc aacagcatct gaatcatccc ttggcgcaac tggccgtgct
     1861 cggtctgctg tgggcctatg cgcatcactt ctgcgccggc atccggcacc tgttgctcga
     1921 tctgcatgtg ggcatcgatc tgcaatcggc gcgcgcctcg gcgcgcgcgg tgctgggcgc
     1981 gagcctcgcg ctgacgctgc ttttcggatg ggtgctgttg tggtgacacg cgcgaagcga
     2041 cgtgtcgtcg gcgcgcatta cggcaccgcc cagtggctgc ggcagcgact gaccgcggtg
     2101 atcatggcgg tgtacaccgt gctcgccatt gtggcgctgc tcatccttca ccccgaaaac
     2161 cacgccgagt ggcgggcgct gttcgcgccc ggctgggtgc gggtcgtcac gctgctgttc
     2221 tttctgagcc tctgctacca cgcctgggtg ggcatgcggg acatcctcat ggactatgcc
     2281 cggccgacca gcctgcggct ggcgctctat accgccgtcg tggtggcgct cggcggttat
     2341 gccgcctggg ccgtacagat catgtggcgg ggctagcgtg acgaaactgg tcgtccggca
     2401 attcgatgcc gtcgtcgtcg gggcgggcgg cgcgggtttg cgcgccgcgc tgcagttgtc
     2461 cgaggccggc ttgcgtgtgg cggtgctgtc caaggtcttt ccgacgcgct cgcacaccgt
     2521 cgcggcgcag ggtggcgtgg gcgcggcgct gggcaatatg ggcccggatt cctggcaatg
     2581 gcatatgtac gacaccatca aggggtcgga ctggctgggc gatcaggacg ccatcgaatt
     2641 catgtgccgc accgcgcccg aggtggtgat cgaactggag cacttcggca tgcccttcga
     2701 tcgcaacgag gacggcacga tctaccagcg tcccttcggc ggccacatgc agaacttcgg
     2761 cgccgggccg gtggtgcagc gctcctgttg cgcggccgac cgtaccggcc acgccatgct
     2821 gcatacgctc taccagcgca acgtccgcgc gcagacgcag tttttcgtgg agtggatggc
     2881 gctcgatctg atccgcgacg cggaaggcca cgtcctgggc gtcaccgcgc tggaaatgga
     2941 aaccggcgag gtgtcgatcc tgcaggcgcg ggccaccctg ttcgctaccg gcggcgcggg
     3001 acgcattttc gcggcgacca ccaatgcctt catcaatacc ggcgacggtc tgggcatggc
     3061 ggcgcgcgcg ggaatcccgc tggaagacat ggagttctgg cagttccacc cgaccggtgt
     3121 ggccggcgcc ggcgtgctga tcaccgaagg cgtgcggggc gagggcgggt atctgctgaa
     3181 ctgcaacggc gagcgtttca tggagcgcta cgccccgacg gccaaggatc tggccagccg
     3241 cgacgtggtt tcgcgcgcca tggcgacgga aatcaaggaa ggccgcggct gcgggcccaa
     3301 gggcgatcac gttctgctca aactcgacca tctgggcccg gaagtcatcg aaaagcgcct
     3361 gccgggcata cgcgaaatcg cccgccagtt cgccaatgtc gatccggtgc gcgacccgat
     3421 cccggtggtg ccgacggttc actatcagat gggcggcatc ccgaccaact accgcggcga
     3481 agtggtggtg cccggcgctg acggaccgag caccgtggtg cccggttttt acgcggccgg
     3541 ggagtgctcc tgcgcctcca tccacggcgc caaccgcctg ggaaccaatt ccctgaccga
     3601 tctgctggtg ttcggtaaat ccgcagggga ctccatcatc gagtacttgc gggcaaaccc
     3661 cgggcataag agcctgcccg ccgaggcggg cgagctgacc caggcgcggc tggcccgcct
     3721 gatgcgcccc gagggcgggg agaatgtgta cgaggtgatg gacgacctgc gccgcgtgat
     3781 gcaggcccat tgcggcgtgt tccgcttccc ggatctgctg accgaaggcg tggagatggt
     3841 gcgcgaactg tcggagcggg tggcccgcac ggcggtacgg gatcgcagcc gcgtgttcaa
     3901 caccgcactg gtcgaggcgc tggagctgga caacctgatc gaggtggcgc gcgcgaccat
     3961 ggtttcggcc gaggcgcgcc gggaaacacg cggcgcgcat gaccgggagg atcatcatca
     4021 ccgggacgac atcaattggc tgaagcacac gctctggtat aaggaagggg atcggctgga
     4081 gtacaaaccg gtcaacctca agccgctgac ggcgacctca ttccccccca aggcgcgggt
     4141 gtactgaggc gtcggcgcaa tcggagagag catgcatctc aggatctacc gctacgatcc
     4201 cgacaaggac gcgaagcctt acatgcagga tttccacgtc gagctggagc cgacggaccg
     4261 catgctgctc gacgtcctgg tgaaagcgca cgaacaggac gacagcttgg cctttcgccg
     4321 ttcgtgtcgc gaagggattt gcggctccga tgccatgaac atcaacggca agaacatgct
     4381 ggcctgcatc accaagatcg accaggtgaa ggagccggtg gtgattcgtc cgctgtccgg
     4441 cctgccggtc atccgcgacc tgatcgtgga catgaccacc ttcttccagc agtacgaatc
     4501 ggtgtcgccc ttcctgcaga acgacgaagc ccggccttcg cgcgagcggc tgcagtcgcc
     4561 cgaggaacgc gagcgcctga atgggctcta tgaatgcatc ctgtgtgcgt gctgcaccag
     4621 ttcctgcccg tcgttctggt ggaatccgga caaattcgtc ggcccggcgg ggctgctgca
     4681 ggcctaccgc ttcatcgcgg acagccgcga tcaggcgttg aacgagcggt tggacaatct
     4741 ggaagacccg tatcggctgt tccgctgtcg taccatcatg aactgtaccg acgtctgccc
     4801 gaagggcctg aacccggccg aggcgatcgg ccggatacgc gagctgctgg cgaggcggtc
     4861 gttgtgatgg cgatggacga ggtggtgcta cgacgactgc gctggcgttg ccggcgcggg
     4921 ctgctggaga acgacctgat gctgggtcgc tttctcgatc ggcacgccga tcgcctggac
     4981 gagcggcgcg tcacgcagtt gcaggcgctg ctggagttcg aggaccacga tttatgggat
     5041 gtgctggcgg ggcgcgccga atgcagcgac cccgccttgc aggacatggt ggaattgatc
     5101 cgcgccggct gaccttgcta cggcgcctcg ggttccacca gggaaccggg cgcggcttcc
     5161 cacacgaaca tcgacggcgc ggtacatccg ccggcagtat gcaaatagga gcggcgcgtc
     5221 cgttcacaag tcagcaatga aggaggtagt aatgcaaccc aacggcaagg cagtgctcca
     5281 gtacggggaa ggaaaatccg ttgaactccc cgtcctcggc ggcaccatcg gcccggacgt
     5341 ggtcgatatc cgaacgctgt acggcaagac cgacatgttc acctacgacc cgggcttcct
     5401 gtcgacggcg agttgtcgtt ccagcatcac atacatcgac ggtgagcagg gcgtgctgct
     5461 ctaccgcggc tatccgatcg agcagttggc gcagcattgc gacttcctcg aagtctgcta
     5521 tctgctgatc tacggcgaac tgcccaatca ggagcagaag gacgcgttca ccgccaaggt
     5581 cgcccgtcac tcgctggtgc acgagcaact ggcgcgtttc tacaccggct tccgtcggga
     5641 ttcgcatccc atggcggtgc tggtcggcac ggtcggcgcg ctgtcggcct tctatcacga
     5701 ctcgctcgac atcagcattc ccgagcatcg cgaaatctcg gcgctgcgtc tgatcgccaa
     5761 gatgccgacc atcaccgcga tggcgtacaa gtactcgatg ggccatccgt tcgtgcatcc
     5821 gcgcaacgaa tattcctaca ccgccaactt catgcgcatg atgttctcgg tgccgaacga
     5881 ggactaccag gtcaacccgg ttctggtgcg cgcgctggac cgcattttca tcctgcatgc
     5941 cgatcacgag cagaacgcct ccacctcgac ggtgcggctg gccggctcct ccggcgccaa
     6001 cccgttcgcg tgtatcgccg ccggcatcgc ctgcctgtgg ggtccggcgc acggcggcgc
     6061 gaacgaagcg tgcctgcaga tgctcgagga aatcggcgac gtgtcgcgcg tgggcgaata
     6121 cgtcaagaag gccaaggaca aggactcggg cttccgtctg atgggcttcg ggcatcgtgt
     6181 ctacaagaac tacgatccgc gcgccaagct gatgcgcgag acctgccacg aggtgctcag
     6241 cgaactgggc ctgcagaacg atcgcctgtt caagctggcg atggaactgg agaagattgc
     6301 gctggaggac gactacttcg tcgagcgcaa gctgtatccc aacgtcgatt tctattcggg
     6361 catcgtgcag cgcgcgctgg gcatcccgac gcagatgttc acctgcatct tcgcgctcgc
     6421 gcgcacggtg ggctgggtgg cgcagtggat ggaaatgatt gccgaccccg atcagaagat
     6481 cggccgtccg cgtcagctct ataccggtgc gacccgtcgc gacgtggtgc cgatggacaa
     6541 gcggtaaagc ttcaccacag aggcacagag acacagagaa aggcaaaaac cttgaaccac
     6601 caaggcacca agacaccaag ttttgtaaga cggtttcttg gtgttctctt ggtgccttcg
     6661 tgtcttggtg gttaaaaaga ttttttctgc gcctctgtgt ctctgtggtt cgaatagtgg
     6721 gcgtcagtgt gccgaggtga gccgatgctg aagcagttcc tggaaaactc ccatctcttc
     6781 ggcggcaacg cgccgttcat cgaagacctg tacgagtcct acctggacaa ccccgactcg
     6841 gtctcgccgg agtggcgcga gtacttcgac cgcatgcaac tgctccccga tcagtcgggg
     6901 ggcgaggcgc gcgatgtggc gcatgcaccg gtcgttcacg catttgccca gcttgcgcgg
     6961 cagcgtagtc tgcgcaccgg tccgccgacg accgagctga gcgtcgagcg caagcagatt
     7021 tatgtgctgt ggctcatcca cgcctatcgc ttcctcggct cgcgttgggc gcagctcgat
     7081 ccgctgaagc gcctgccgcg gccccacatt cccgaactcg aaccggctta ctacgacctc
     7141 accgaggccg acctcgagac ggagttcaac accggtacgc tgatcggccc cgagcgcatg
     7201 accctgcggg aaatcctgca ggcgttgcgc gaaacctatt gcggcaccat cggcgccgag
     7261 tacatgtaca tgagcgatcc gacgcagaag cgttggatcc agcagcgcct ggagtccatg
     7321 cgctcgaagc cgaacttcgg cgccgacgtg cgtacgcgta tcctggagcg cctgactgcg
     7381 gccgaaacgc tggagaagca cctgcacacg cgctatgtcg ggcagaagcg tttttcgctc
     7441 gagggcggtg agacgctcat tccggtgatg gacgaactgg tgcgacgcgc gggcgcctac
     7501 ggcatcgagg aagtggtcat cggcatggcg caccgcggcc gcctgaacgt gctggtcaac
     7561 acgctgggca agtcgcccaa ggatctgttc tcggaattcg agggcaaggc ggtgagcgag
     7621 ttgtcctccg gcgacgtgaa gtaccacaac ggcttttcgt cggacgtcgc cacgccgggc
     7681 gggccggtac atctgtcgct cgccttcaac ccgtcgcacc tggaaatcgt caacccggtg
     7741 gtggaaggct cggtgcgcgc gcgtcagcat cgtcgcaagg atcattcggg cgacaaggtg
     7801 ctgtcggtgc tgctccacgg cgatgccgcc ttcgcggggc agggcgtggt gatggaaacg
     7861 ctcaacctcg cgcagacccg cggctacggc accggcggca cggtgcacat catcatcaac
     7921 aaccagatcg gtttcaccac ctcggatccg cgcgatgcgc gttcgacgat ctactgcacc
     7981 gacgtggcga agatgatcga agcgcccatc ttccacgtca acgccgacga tccggaagcg
     8041 gccgtgttcg tgatgcagct cgccatggac ttccgcaagg agttcaagaa ggacgtggtg
     8101 gtcgatctga tctgtttccg caagctgggg cacaacgaac aggacgagcc cttcgtcacc
     8161 cagccgctga tgtacaagaa gatcgccgag catccgggca cgcgccagct ctacgccgat
     8221 cggctggtcg cgcaggacgt catctcgccc gaggagccgg aggaattgat caggcaatgc
     8281 cgccaggcgc tcgacgaagg gcggcatacc tccagtcccg tgctgtcgaa ctaccagcga
     8341 aagtatgcgg tggactggac gccgttcctg aacaccaagt ggacggaggt ggccgatacc
     8401 accgtgccga tcgccgaatt ggagcggctg gcggagcgca ttaccaccgt gcccgaggac
     8461 ttcacgctgc accccacggt gcagcgcgta atcgattcgc ggcgtgccat ggggcgcggc
     8521 gaacagccgc tcgactgggg catgggagag acgctggctt atgccagtct gctggtcgcc
     8581 ggctacggcg tgcgcatctc cggtcaggac tcgggacgcg gcactttcgc gcaccggcac
     8641 gccgtgctgc acgaccagga ccgcgaacgc tgggacgcgg gcacctatgt accgctgtcc
     8701 aatctgcagg aaggccaggc cgatttcgtg gtcatcgatt cggtgctgtc cgaagaggcg
     8761 gtgctgggct tcgaatacgg ttacgcctgc gccgaaccga acgaactggt cgtctgggaa
     8821 gcgcagttcg gcgatttcgc caatggtgcg caagtggtca tcgaccaatt catcacttcc
     8881 ggtgaggcga agtggggccg catgtgcggg ctggtgatgt tcctgccgca cggctatgaa
     8941 gggcaggggc ccgagcactc ctccgcgcgc ctggagcgct acctgcagct ttgcgcggaa
     9001 cacaacatcc aggtgtgcgt gcccagcacg ccggcgcaga tcttccatct gctgcgccgc
     9061 cagatgctga ggccgttccg caagccgctg gtggtgatga cgcccaagtc gctgttgcgc
     9121 cggaaggaag cctcctcgca actggcagag ctggccgaag gctgcttcca gaccgtgatc
     9181 agcgaagtcg aggatctcga cccgcagcag gtcacccgca tcattgccac ttcggggcgc
     9241 atctactacg acctggcggc ggcgcggcgc gagcgcggcg cagaccatgt cgcgatcatc
     9301 cgggtcgagc agctctatcc cttcccgcac gagcattttg ccgcggagat gcgcaagtac
     9361 ccgaaggcga aggaggtggt ctggtgtcag gaagagccgc agaaccaggg tgcgtggtac
     9421 cagacgcagc actatttcct ggagaacatc cgcgaggacc agaagctcta ctacgcaggg
     9481 cggatgtcca gcgcttcgcc ggcggttggc tatttggcca agcacaacga acagcagaag
     9541 aatctgctcg acatggcttt cggcccgttc aagaattgag tcgcgagtga ggtgggtgcc
     9601 ggtgcatggt gcgcggtttt gcggttggag gagtgcgctc gaacgcttag gatggttgcc
     9661 aacgagcgaa gccgtaccga agccgtacga gtggggcgaa gcaggggccg ggccttgaga
     9721 cggaacttgc aagcggattg ttcatggacc tcgatcgaag ttcctctgcg tgagggcagc
     9781 gtccagtcga gtcgggcgta aaaccaaacg gagtgatcat gcttgtagaa gtgaaagtgc
     9841 cgcagttgtc ggagtcggtg tccgaagcga cattgctcac gtggcataag aaggaaggtc
     9901 aggcggtcaa gcgcgacgaa aacctgatcg acatcgaaac cgacaagatc gtcatggagt
     9961 tgcccgcgcc ggccgacggc gtgctgacca agatcgtcaa gcaggacggc gacgtggtga
    10021 cttccggcga ggtgatcgcg accatcgata cggaagccaa gcccacccaa agcgagagcg
    10081 cgacggccaa agcggaagcg cccgtacagg cttcggcgca accgcaaccg gcgctgcatc
    10141 ccgagccggc gctggtcgag cccacgcccg cggccgcgcc ggccgaggcg cgccaggacg
    10201 cccggctgat gcctgcagcc cgcaagctgg tcgaagaaca caacgtcgat cccagccgca
    10261 tcgccggcag cggacgcgac ggccgcatca ccaaggccga tgtgctcgat catctggcca
    10321 aggcaccgca gccggcccag gccgaagcga agccgtcgct gccgcccgcg ccgccggtga
    10381 atctcgatca ggtgctggcc ggccggcccg agcagcgcgt gccgatgtcg cgactgcgcg
    10441 cccgcgtcgc cgagcggctg gtgcagtcgc agcggacggc cgcgatcctg accaccttca
    10501 atgaagtgaa catgaagccg gtcatcgatc tgcgcaagaa gtacggcgag cgcttccagg
    10561 gggaacacgg cgtcaaactg ggcttcatgt ccttcttcgt caaggcggcg gtgcatgccc
    10621 tgaagaagta tccgatcgtc aatgcttcgg tggacgggaa cgacatcatc tatcacggct
    10681 acttcgatat cggtattgcc gtcagcagcc cccgcggtct ggtggtgccg atcctgcgcg
    10741 actgcgatca actgtccgtc gccgaaatag agaagaagat cgccgattac ggccagcgtg
    10801 cgcaggaagg caagctgagc ctggaggaac tggccggcgg caccttctcc atttccaacg
    10861 gtggcgtatt cggttcgatg ctttccacgc ccatcatcaa tccgccgcaa agcgccatcc
    10921 tcggcatcca tgccacacgc gatcggccgg tggtggaaga cgggcaaatc gtcgtgcggc
    10981 cgatcaacta tctggcgctg tcgtacgacc atcgcatcat cgacggccgc gaagcggtgc
    11041 tggcgctgat ggcgatcaag gaagcgctgg aagagcctgc gcgactgctg ctggatcttt
    11101 aacagcagat ctttaaccac caagacacca agggcaccaa gcgttccgat gagggctctc
    11161 ttggtgtctt ggtgccttgg tggttcaagg ttttgagagt cttgaaatga gtgatcaatc
    11221 cttcgatgtc gtcgtcatcg gttccggtcc cgccggctac atctgcgcga tccgcgcggc
    11281 gcaactgggg ttcaagaccg cttgtgtcga ggcatggcgc aatcccgccg gcaagcccgt
    11341 gctcggcggc acctgcctca acgtcgggtg cattccctcc aaagcgctgc tgcagtcttc
    11401 tgaagagtac gaccacgtca acaaggggct cgccctgcac ggcatcgagg tgagcggcgc
    11461 aagcatcgac gtgcctaaaa tgatcgcgcg caaggatgcg atcgtcagca agatgaccgg
    11521 cgggatcgag ttcctgttca ggaagaacaa ggtcagctgg ctgcagggcc acggccggct
    11581 ggtaaagcac agcgagcagg gctggcagtt ggatgtggcg ggcaaggccg tacaggcgaa
    11641 gcacgtcgtg atcgctaccg gctccaagcc gcgcaccctg ccgggcgtgg agatcgacaa
    11701 tgagctcgtg tgcgacaacg tcggcgcgct ggatttcaag acggtgccgc agaagctcgg
    11761 cgtcatcggc gccggggtga tcggcctgga actgggcagc gtgtggcgcc ggctcggcgc
    11821 cgccgtgaca gtgctggaag ccttgccgga gttcctgccc gcggccgacg agtcggtctc
    11881 gcgcgaagcc tggaagatct tcaccaagga gcagggcctg gatatccggc tgggcgtgaa
    11941 gctgggcgag gtgcggcgcg gcaagaacaa tgtgagtgtc gtgtgcgagc acgacggtcg
    12001 ccgggaaacg ctggagttcg acaaactcat cgtcgccatc gggcgggtgc cgaacaccga
    12061 cggcctcggc gccgaaggcg tggggctcca gtgcgatgcc cgcggcttca tcgaagtgga
    12121 cagccattgc gccaccagcc tgcccaatct ctatgccatc ggcgacgtgg tgcgcgggcc
    12181 catgctggcg cacaagggcc aggaggaggg tgtcatggtg gccgagcgta tcgccggcca
    12241 gaagccgctt gtcgatctgg agaccattcc ctgggtgatc tacaccgcgc cggaaatcgc
    12301 ctgggtcggc cagaccgagc agcagctgaa ggcggaggga attccctatc gcgccgggca
    12361 gtttcccttc tccgtcaacg ggcgtgccct gtgcgcgggc gatccgcgcg gcttcgtcaa
    12421 aatgctggcg caccggaaga ccgacgagat tttgggtgtg cacatcatca acacgcatgc
    12481 ttcggaattg atcgccgaag cggtggtggc gatggaattc cacagcaccg ccgaggacat
    12541 cgcgcgcatc gtgcatgcgc acccctcgct caccgaagcc ctgcacgagg cggcgctggc
    12601 agtggacaag cgggcgttga atatctgaac gccacgcttc ggtgcgtgcg atttttgcgc
    12661 gctgaggccg tgcgcgaccg ccgttgtgcg gtaatcttcc ggggtcggac tcagcgctag
    12721 cgtcaatgca cgaccgccag atcgaagtcg agagctatca gccgctgccg cccatcgggg
    12781 acggcagtgt gttcgatgca gccgagtaca ccaagcccgc cgtcaggctg ccgctcaccg
    12841 tactcgaata cttccatgac accctcgcgc gccgcggcta tgtcccggac gaatcccagt
    12901 atgccgcggt gcagcgcctc cagcgcatgt acgaacagtg gatggattac gacgcgcgca
    12961 gcaagaacct ctggaagcgc tttctcctgc agcccacacc gccgcgcggc gtttatctgt
    13021 ggggcggcgt cggacgcggc aagagcctcc tgatggatac cttctatctg accgtgccgg
    13081 aggagcgcaa acggcgcgtg cacttccatc acttcatgcg tgacgttcac cgcgagatgg
    13141 agacgctcaa gggcaaggag gatccgctgg cggcagtcgc ctcgcgcatt gcgcgccgct
    13201 accggctgat ctgcttcgac gaattccatg tggacgatat cgcggatgcg atgattctcg
    13261 gccgtctgct cgagcagacc atggaccgcg gcgtggtcta ttgcatgacc tccaactatc
    13321 accccgacgc cctttatcgc cacggactgc aacgcgaacg ctttctgccc accatcgcgc
    13381 tgatcaagga gcgcctggac gtggtgcacg tggatggcgg cgtggattac cgccgccgcg
    13441 cgctgcaagg cgtggagatc taccactgtc cgctcggtcc ggaggcggat gccgcgctgt
    13501 ggcgggcttt cgagcagatc gcggaagtgg aggaggagca tcacgaactg gacgtcgagg
    13561 gacgtgtcat tcccttcgaa caccgcgccg gcggcgtggt gtggttcgac ttcaacgtgc
    13621 tgtgcggcgg cgcgcgttcg cacaacgatt atctcgatct ggccaagcgc ttccacacgg
    13681 tgatcgtgtc caatattccg cagttgaccg aggacgatgc cgacaaggcg cgccgcttca
    13741 cctggctggt ggacatcttc tacgatcgca aagtgaagct ggttgtctcg gcggcggtgc
    13801 cgcccgagca gctttatcga gacggcccgt tcgtcaacga attcgcccgc accgcgagcc
    13861 ggctgatcga aatgcagtcg aacgaatatc tggcggcgtc gcgccgcgat ccgcaagcgg
    13921 ctgccattgc ggcttgaacc ccaggagacc gaacacatgc gccaacgtgc ctgcctgacg
    13981 atcgaagatt gcaaacgtat tgcggccgcg gcggaagccg aagccctgaa gaacaagtgg
    14041 gaagtggcgg tcgcgatcgt cgacgacggc ggccatctgc tgcatttcca gcgcatggac
    14101 gaagcgacgc ccgccaacgc ggagatcgcg ctcggcaagg cgcgcaccgc ggcgatgtcg
    14161 cgccggccgt ccgccatgtg ggaggagcgc atcaagaacg gccgcctgtc catgctcggc
    14221 atgccgctgc tgccggtgca gggcgggctg cccatcatcg tggacggcgt ttgcatcggc
    14281 ggcgtcggcg tgtccggcgt gcaatcgcac gaagacgaac agatcgcgca ggcgggcatc
    14341 gacgcactga ctgcctgaac gcgcagccgg cgttcgggcg acacgaatct ggcgacgacc
    14401 cggtcgagcc ggacttcatc gtcgtcgcgt ataggcacga ttgaggagca ggccatggcg
    14461 catcgaatcg aacgcgacac cttcggcgat atcgaagttc cggccgagcg cctgtggggt
    14521 gcgcagacgc agcgttcgct gcagaacttc cgcatctccg gcgaaagaat gccggcggaa
    14581 ctcatccgcg cgctcgcgct ggtgaagcgc gcctgtgccg cggtcaacaa ggaactggga
    14641 caactggacg acgccaaggc ggacgccatc gtgcgcgccg ccgacgaagt gctggcgggc
    14701 gatcatcccg acgaattccc gctggtggtg tggcagaccg gctccggcac ccagagcaac
    14761 atgaacatga acgaggtgct cgccaaccgc gcctcggaac tcctgggcgg cgagcgcggc
    14821 gaagggcgca aggtgcaccc gaacgacgac gtcaatcgcg gccagtcgtc caacgatgtc
    14881 tttcccacag cgatgcatgt cgccgccgtc gaacagatcg agcggcgttt gctgccggcg
    14941 atcgaaaggc tgcgcgatag cctgcgcgaa aagtcggccg ccttcgccga catcgtcaag
    15001 atcggccgca cgcatttgca ggacgcaacg ccgctcacgc tgggtcagga gttttccggc
    15061 tatgtcgcgc agctcgatca tggcctcagg catgtgcgtg acgcgttgcc gcatctgagc
    15121 gaactggcgc agggcggcac ggcggtcggc acgggattga acgcgcatcc cgagttcggc
    15181 gagcgcgtcg ccgcggaatt gagccgcctg accgggctgt ccttcgtgtc cgcgccgaac
    15241 aagttcgagg cgcttgccgc gcacgatgcg ttggtgcacg ggcacggcgc gttgaagacg
    15301 cttgccgcgt cgctcaccaa aatcgccaac gatgtgcgct ggctcgcttc cgggccgcgt
    15361 tcgggattgg gggagatcac catcccggag aacgagccgg gcagttcgat catgcccggc
    15421 aaggtcaatc ccacgcagtc cgaggcgttg accatggcgt gcgcacaggt attcggcaac
    15481 gatgtggcgg tgaacttcgg cggcgcgcag gggaatttcg aactgaacgt ctacaagccg
    15541 ctgatgatcc acgcgttcct gcagagcgtg cggctgttgg ccgacggtgc ggaaagcttc
    15601 gacgcgcatt gcgtgcgcgg catcgaagcc aatcgcgagc gcatcgacga gttgctgcac
    15661 cgctcgctca tgctggtgac ggcgctcaat ccgcacatcg gttacgacaa ggcggcacag
    15721 attgcgaaga aggcgcatcg cgacggcagc acgctcaagg aggcggcagt cgcgctcggt
    15781 ttcgtcagcg aagcggactt cgacaagtgg gtgaaagcgg aagaaatggt gggcagacgc
    15841 tgattcctcg aggggcttcg cttagaatgc gagcttttgt ccttagagaa gcagttcatg
    15901 tccagcgtag cgccatcccg cagtacctcg aagaccaagt cctcctccgc tcagtctccc
    15961 aagcgatcca ccgcgaagtc caatggcgca accaggccgg tgtcgaagcg cagtgtcgcg
    16021 aagaaggccg cgctggagaa gctctatgtg gtgcgctggt cgaagatcca tggccgcggc
    16081 gtgtttgccg ccaccgacat tcccaagggc acgcgcatcg tcgaatacaa gggcaagcgc
    16141 atctcctaca agcttgcctg caggctttat cccgatgtgg acggcaagcc cacccacacc
    16201 ttcctgttcg agatcgacga cgacacggtg atcgatgcgg cgcagggcgg caacgcggcg
    16261 cgctggatca atcactcctg cgagcccaac tgcgaggcgg tggaggagga cggccgcatc
    16321 tacatcgagt ccacgcgcaa gataaaggcg ggcgaggaac tgggctacaa ctacggcatt
    16381 acgctggacg agccgcatac gaagaaagag aaggcgcgct ggccctgcta ttgcggcaag
    16441 aaatcctgcc gcggcacgct gctccagccg aagcgctgag actgtactgc cgggcgccgt
    16501 gaggcgcccg gtttacaaga gggcgcgatt ggtgcttcca ttattggccg ccggaccgcg
    16561 tccaaccata acaccggccc cacaccagac aggaggcaag catgtcggtc accatccaat
    16621 cggtgctcaa cgaggaacga ctctttccgc cgccggccga tttcgtcaag caggcgaaca
    16681 tttccggcat ggccgcctat gaagcgctat gccaggaagc cgaacgcgac tatgagggct
    16741 tctgggcgcg tctggcgcgc gagcatctgc tgtggcaaaa gccgttcacg acggtgctcg
    16801 atcagagcaa tgcgccgttc ttcaaatggt tcgacgacgg ccagttgaat gcctcctaca
    16861 actgcctgga ccgtcacctc gatacccagc cggacaagac cgcgatcatt ttcgagacgg
    16921 atgacggaca ggtcagccgc atcacctatc gcgaactgca ccgccgcgtc tgccgcttcg
    16981 ccaatacgct caagggccat ggcgcgaaga tgggcgatcg aattctgatc tacatgccga
    17041 tgtcgatcga ggtggtggtc gccatgcagg cctgcgcgcg tatcggcgcc attcattccg
    17101 tagtgttcgg cggcttctcc gccaagagcg tgcatgagcg gatcgtcgac gtcggcgcct
    17161 cgctcatcat caccgccgac gggcagatgc gcggcggccg cgagattccg ctcaagccgg
    17221 cggtggacga ggcgattgcg atgggcggct gcgagagcgt gcgcaacatc atcgtctatc
    17281 gccgcaccgg tactgccgtc gccatgcagg cgccgcgcga cgtgtggtgg cacgacgtga
    17341 tcgagggaca gcccgaggtg tgcgagccca cctgggtgaa cgccgagcat ccgctctaca
    17401 ttctttacac ctccggttcg accggcaaac ccaagggcgt gcagcactcc accgccggct
    17461 tcctgctgtg ggccaagctg acgatggact ggaccttcga cctgcaaccc gaggacgtgt
    17521 tctggtgcac cgccgatatc ggctgggtca ccgg
//
LOCUS       DATGOU010000095         6264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_2060728, whole genome shotgun sequence.
ACCESSION   DATGOU010000095 DATGOU010000000
VERSION     DATGOU010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2060728"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VKZ48_05785"
     CDS             complement(<1..862)
                     /locus_tag="VKZ48_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLU77396.1"
                     /translation="MNVDVENQTDIRTAAGRPDAAPKARPTRRIALIIGAVVLLAGAA
                     YFAYSQWFVGTRPADRYTTAVVTRGDIEDTVTATGTLQPRDYVDVGTQVSGQLKVVHV
                     EIGSKVKQGDLLAEIDPTVYRSRVDANRAQLQNQEAQLADRQAQLQLAQQQLRRQQNL
                     MKEQATTEEALQTAEAAVRSYTAQIAALKAQMQQTQSELRGNEANLGYTKIYAPMAGT
                     VVSQTAKQGQTLNANQQAPIILRIADLSTMTVQTQVSEADVPKLRLGMDAYFTTLGGG
                     GQRWHGTLRQA"
     gene            1105..2565
                     /locus_tag="VKZ48_05790"
     CDS             1105..2565
                     /locus_tag="VKZ48_05790"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLU77397.1"
                     /translation="MSGTTDTVATPAASAPASLLGRRFDRLRVKLFLAIAGANAAVAI
                     IAYLLFSLSFDRGFIEYLHRSDVARLDAVVSELAEAYARDGSWEAVAADREGWSDRSR
                     RAFGLPRRGDATPDVTQRAARRDFPLTIDPRLMLFDAGQQLLVGRPEHAEAAQLKPIE
                     VRGEVVGYLGYVQRHWLFESVARLASAQQHGGFAAIGIGMLGAALLLGAGLAHWLTRR
                     IRALGAGTAALTRGDYAVHIESSGHDELAQLARDFNQLAATLAATRRARSQWVADIAH
                     ELRTPLAVLRGEIEALQDGVRPLNADSLESLAHEAARLGRLVEDLHLLSMSDLGALTY
                     YTEPVDMAEIVEEVLKVHAGALADHPLHVKTSLQRAMVMADAARLAQVYGNLLQNSLR
                     YTDAGGRLAVSVYADADRVIVRWEDSAPGVPADELERLTDRLYRVDGSRSRAGGGSGL
                     GLAIVKAIVDAHHGSMRAEASALGGLAWQMEFPRHG"
     gene            2558..3229
                     /locus_tag="VKZ48_05795"
     CDS             2558..3229
                     /locus_tag="VKZ48_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLU77398.1"
                     /translation="MAERILIVEDEPKIAELLRDYLRQESYEVDVLDRGDTVEAWVRE
                     HGADVVLLDLMLPGKSGLEVCKGLRTFSDAAIIMITARVDEIDRLLGLELGADDYICK
                     PFSPREVVARVKAVLRRIRRQDRPQDSGGLVLDEAGCKALIEGRDLALTVVEFQLLKV
                     LASRPGRLYSRDQLMDAMYRDARVVSDRTVDSHIKKLRRKIADVLPDRELIHSVYGVG
                     YKYEW"
     gene            complement(3390..5321)
                     /locus_tag="VKZ48_05800"
     CDS             complement(3390..5321)
                     /locus_tag="VKZ48_05800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLU77399.1"
                     /translation="MTPLTESQELLILESDARLQLALAAGRMGIWEWDSATNKVAWNG
                     TEQRIAGRDRHSLTPEQFFEVVHPDDRERVEVAFRAAVTQQASFEAEFRIIRSSGEER
                     WLVGRGMPLVGRDGRTAKVIGVNFDITDRKRSEQARQRAETALRDAHVLIRAITDNAT
                     TAIFMVDGQGLTTFMNPAAEAMVGYRFDEVRGLPLHAVTYAPLPDGRPMQLEGCCIGA
                     SIRTQCAVREQEEIFVNRAGEAFPVRCNASPVFTDDVLSSVVLEVRDITAEKRSQAAL
                     EEADRRKDRFLATLAHELRNPLAPIRNALHALERDTGPERVEWAKRLMSRQVAQLVRL
                     IDDLLDISRITQDRMTLRRESVALAEVVDCAVETSEPALVERGHRFMLELPPEPIRVH
                     ADATRLAQILANLLNNAAKYSDPGAEIRLSAAVEDGMAVLRVRDTGRGIAPEMLPHIF
                     GMYAQADRKHMPTGDGLGIGLALVKKLVDLHEGRIEAYSAGVGCGSEFVVHIPLAAPN
                     EPAVAEDVAAPPAAPSRALRVLVVDDNPDAADSLMFALAGAGHEASAAYNGREALDAA
                     NTFKPDAIVLDIGMEDIDGYDVARRIREQSWGADITLIALTGWGQAEDRARALEAGFD
                     HHVIKPADPLDLMQRLARV"
     gene            complement(5545..5620)
                     /locus_tag="VKZ48_05805"
     tRNA            complement(5545..5620)
                     /locus_tag="VKZ48_05805"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5585..5587),aa:Thr,seq:tgt)
     gene            complement(5663..6112)
                     /locus_tag="VKZ48_05810"
     CDS             complement(5663..6112)
                     /locus_tag="VKZ48_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HLU77400.1"
                     /translation="MTEPTSTKPYLMRALYEWCVDNGYTPYISVVVDANTRVPTEYVR
                     NGEIVLNVGPLATNRLKIGNDYVEFSARFGGVARELIIPVSQVSAIYARENAQGMSFP
                     VEREADETDVAPASAEEAPASAAQDSDEPPVPPTNNGGGKPTLRIVK"
BASE COUNT         1027 a         2005 c         2091 g         1141 t
ORIGIN      
        1 gcgcctgccg cagcgtgccg tgccagcgct gcccgccgcc gccgagcgtg gtgaagtagg
       61 cgtccatgcc gaggcgcagc ttgggcacgt ccgcctcgga aacctgcgtc tgcaccgtca
      121 tggtggacag atcggcgatg cgcagaatga tcggcgcctg ctggttggcg ttgagcgtct
      181 ggccctgttt ggccgtctgc gagacgacgg tgccggccat gggcgcgtag atcttggtgt
      241 agccgaggtt cgcttcgttg ccgcgcagtt cggattgcgt ctgctgcatc tgcgccttca
      301 gcgccgcgat ctgcgcggtg taggaacgca ccgccgcctc ggccgtctgc agcgcttctt
      361 cggtggtcgc ctgctccttc atcagattct gctggcgccg caactgttgc tgcgcgagtt
      421 gcagttgcgc ctggcggtcc gccaactgcg cctcctggtt ctgcagttgc gcgcgattgg
      481 cgtccacgcg ggagcgatag acggtcggat cgatttcggc gagcagatcg ccctgcttca
      541 ccttcgaacc gatttccaca tggacgacct tcaattggcc ggagacctga gtgccgacgt
      601 cgacataatc gcgcggctgc agcgtgccgg tggcggtgac ggtatcttcg atgtcgccgc
      661 gggtgacgac cgccgtggtg tagcgatcgg cgggacgcgt gccgacgaac cactggctgt
      721 aggcgaaata ggcggcaccg gcgagcagca cgaccgcgcc gatgatgagc gcaatgcgcc
      781 gcgtcgggcg cgctttcggg gcggcatccg ggcggccggc agcagtgcgg atatcggtct
      841 ggttttccac gtccacgttc atggggcgag aaccggttgg cattgatgtg cgacggattg
      901 tgacgcagga tgccgcacga actgtgcacc aaccgtgcaa atccggtttc ggcgggtttc
      961 gggagaggaa tagttgtgca tccatgccgt ccacgcaagc cgggccgcaa cgcgacccgg
     1021 agcggcgatc gggccggcgt ccacaaagct tccgcgattt cgcgctagtc tccggcgcat
     1081 ggacatactc cagcccttat ggacatgagc ggcacaaccg ataccgtagc cacgccggcg
     1141 gcctcggcgc cggcctccct cctgggacgg cggttcgacc gcctgcgcgt caagctgttc
     1201 ctcgccatcg ccggcgccaa tgccgcggtg gcgatcatcg cctatctgct gttcagcctg
     1261 agcttcgacc gaggcttcat cgagtacctg catcgcagcg acgtcgcccg gctcgatgcc
     1321 gtcgtgagcg aactggccga agcctacgcc cgcgacggca gctgggaggc ggtggcggcc
     1381 gatcgcgaag gctggtccga ccgttcgcgc cgcgccttcg gcctgccccg ccgcggcgac
     1441 gcgacaccgg acgtcaccca gcgcgcggcc cgtcgtgatt ttccgctcac catcgatcct
     1501 cgcctgatgc tgttcgacgc cgggcagcag ttgctggtcg gccgcccgga acacgccgag
     1561 gcggcgcagt tgaagcccat cgaggtgcgc ggcgaagtcg tcggctatct gggctatgta
     1621 cagcggcact ggctgttcga gtcggtggcg cggctggcgt cggcgcaaca gcacggcggc
     1681 ttcgccgcca tcggcatcgg catgctcggc gcagcgctgc tgttgggcgc cggactggcg
     1741 cactggctga cgcggcgcat ccgcgcgctg ggcgccggca ccgccgcgct cacgcgcggc
     1801 gactacgccg tgcacatcga gtcgagcggg cacgacgaac tcgcacagct tgcgcgcgac
     1861 ttcaatcaac tcgccgccac actggccgcc acgcgacggg cgcgcagcca gtgggtggcc
     1921 gacatcgcac atgaactgcg cacgccgctg gccgtgctgc gcggcgagat cgaagcgctg
     1981 caggacggcg tgcgcccgct caacgcggac agtctggaat cgcttgcgca cgaggcggcg
     2041 cgactgggcc ggttggtgga ggatctgcat ctgctgtcga tgtccgatct gggcgcactc
     2101 acttactaca ccgagccggt cgacatggcg gaaatcgtcg aagaggtgct caaggtgcat
     2161 gccggcgcgc tggccgatca cccgctgcat gtaaagacct cgctgcagcg cgcaatggtg
     2221 atggccgatg cggcgcggct cgcgcaggtg tacggcaatc tgctgcagaa ttcactgcgc
     2281 tataccgatg cgggcggccg gctcgccgtc agcgtgtacg cggatgcgga tcgcgtcatc
     2341 gtacgctggg aggattccgc gcccggtgtg ccggccgatg aactggagcg tctgaccgac
     2401 cgcctctacc gcgtcgacgg ttcgcgcagt cgcgcaggcg gcggctcggg tctgggtctg
     2461 gcgatcgtta aggccatcgt cgatgcgcat cacggcagca tgcgcgcgga ggcaagcgca
     2521 ctgggcggcc tcgcctggca gatggagttt ccccgtcatg gctgaacgca ttctcattgt
     2581 cgaagacgag ccgaagatcg ccgagttgct gcgcgattat ctgcgtcagg aaagctatga
     2641 ggtggacgtg ctcgaccgcg gcgacacggt ggaagcctgg gtgcgcgagc acggcgcgga
     2701 cgtggtgctg ctcgatctca tgctgcccgg caaaagcgga ctggaagtgt gcaagggcct
     2761 gcgcactttc agcgatgcag ccatcatcat gatcaccgcg cgcgtcgatg aaatcgaccg
     2821 cctgctcggg ttggaactgg gcgcggacga ctacatctgc aaacccttca gcccgcgcga
     2881 ggtggtggcg cgggtgaaag cggtgctgcg tcgcatccga cggcaggacc ggccgcagga
     2941 cagcggcgga ctggtgttgg atgaagccgg ctgcaaggcg ctcatcgaag gacgcgatct
     3001 cgctctcacg gtggtggagt tccaactgct gaaagtcctc gccagtcggc cggggcgctt
     3061 gtacagccgc gaccagttga tggacgcgat gtatcgcgat gcgcgcgtcg tgtccgaccg
     3121 caccgtcgac agccacatca agaagctgcg tcgcaagatt gccgatgtgc tgcccgaccg
     3181 ggaactgatc cactccgttt acggcgtcgg ctacaaatac gagtggtagg cgcaagcgtg
     3241 ctgggccggc tgcgtgatgc ggacgccttg aaaccttgaa tccagaggac gcggcgtaca
     3301 cagagtaaag cgttcactcc gcgtccccga ggattagcgg aaagaatttc ctggtgcctt
     3361 ggtggttcaa gccttttgtt tttcgatcgc tatacacgcg ccagccgttg catcagatcg
     3421 agcgggtcgg ccggtttgat cacatgatga tcgaagcctg cttccagtgc tcgcgcgcga
     3481 tcttccgcct ggccccagcc ggtcagtgcg atcagtgtga tgtccgcgcc ccaactctgt
     3541 tcacggatgc ggcgcgccac atcgtagccg tcgatgtctt ccatgccgat gtcgagcacg
     3601 atggcatccg gcttgaaggt attcgcagcg tcgagcgcct cgcggccgtt gtaagcggcg
     3661 ctcgcctcgt gccccgcgcc ggcgagcgcg aacatgagcg aatcggcggc gtccgggtta
     3721 tcgtccacga ccagtacgcg taatgcacgc gacggcgcgg ctggcggcgc cgcgacgtcc
     3781 tccgccacgg ccggttcgtt cggcgccgcc agcggaatgt gcacgacgaa ttcgctgccg
     3841 caaccgacgc ctgcgctgta cgcctcgatc ctgccttcgt gcaaatcgac cagcttcttc
     3901 accagcgcga gcccgatgcc gagaccatct cccgtcggca tgtgctttct gtcggcttgc
     3961 gcgtacatgc cgaagatatg cggcagcatt tcgggcgcaa tgccgcgccc ggtgtcgcgc
     4021 acgcgcagca ccgccatacc gtcttcgacg gcggcgctca accggatctc ggcacccggg
     4081 tcgctgtact tggcggcatt gttgagcagg ttcgccagga tctgggccag gcgcgtggcg
     4141 tcggcgtgca cccgtatcgg ctccggcggc agttcgagca tgaagcgatg gccgcgttca
     4201 accagcgccg gttcgctcgt ctccaccgca cagtccacca cttcggccaa tgccaccgac
     4261 tcgcggcgca aggtcatcct gtcctgcgta atgcggctga tgtcgagcag atcgtcgatc
     4321 agccggacca gttgcgcgac ctggcgactc atcagtcgct tcgcccactc gacgcgctcg
     4381 ggaccggtgt cgcgctccag cgcgtgcagc gcattgcgga tgggtgcaag cggattgcgc
     4441 aactcgtgcg caagcgtggc gagaaagcgg tccttgcgcc gatcggcttc ctccagcgcc
     4501 gcctgcgagc gtttttccgc agtgatgtcg cgcacctcga gcaccacgct ggacagcaca
     4561 tcgtcggtaa acacggggct ggcattgcaa cgcacaggga atgcctcgcc cgcgcgattg
     4621 acgaagattt cctcctgctc gcgcaccgcg cactgcgtgc ggatgctcgc gccgatgcaa
     4681 cagccttcca gctgcatcgg ccggccgtcg ggcagcggcg catacgtcac ggcatgcagc
     4741 ggcagcccgc ggacttcgtc gaagcggtaa cctaccatcg cttccgccgc aggattcatg
     4801 aaggtggtca ggccctgtcc gtccaccatg aaaattgccg tggtggcgtt gtcggtgatg
     4861 gcgcggatca gcacgtgcgc atcgcgcagc gcggtctcgg cgcgctggcg cgcctgctcg
     4921 ctacgcttgc gatcggtgat gtcgaagttg acgccgatga ctttggcggt gcgaccgtcg
     4981 cgaccgacga gcggcatgcc gcgcccgacc agccagcgct cctcgccgga agaacggatg
     5041 atgcggaact ccgcttcgaa cgacgcctgt tgcgtcaccg ccgcgcgaaa agcgacttcg
     5101 acgcgttctc gatcgtccgg gtggacgact tcgaaaaact gttccggcgt gagggagtgc
     5161 ctgtcgcgac cggcgatgcg ttgctcggtg ccgttccagg ccaccttgtt ggtggcggaa
     5221 tcccattccc agatacccat gcggccggcg gcgagcgcca attgcagccg cgcgtcgctt
     5281 tccaggatca gcaactcctg gctttccgtc agaggggtca tgcagcagct tctactcgca
     5341 aacttcgcga gatgtagtcg ttatggttga actatataaa ccagaaagtc gctgcgagtc
     5401 attaaagcgg gacggttatg gcgcagcctt tgacgattca caaggatgag gcgcgcaaag
     5461 tgtccggcag gcacgacact cctgcgtttg cgcgagtgca aaacggctgc gtgcagtgaa
     5521 tgagttatgg aagagacgcg aagatggtgc cggctagagg aatcgaaccc ctgaccttct
     5581 gattacaaat cagctgctct acctgctgag ctaagccggc gccggaggga ttatacgcag
     5641 cgcgcaggag cgttgccggc cgctatttga cgattctcag tgtgggcttg ccgccgccgt
     5701 tattggtcgg cggcacgggc ggctcgtcgc tgtcctgcgc cgcggacgcg ggcgcctctt
     5761 cggcgctcgc cggtgcgacg tcggtctcgt cggcttcgcg ctcgacgggg aaggacatgc
     5821 cctgcgcatt ctcgcgcgcg tagatggccg acacctgcga tacgggaatg atcagttcgc
     5881 gcgccacacc gccaaagcgg gcagagaact cgacgtaatc gttgccgatc ttgagccggt
     5941 tggtcgccag cggcccgacg ttcaacacga tctccccgtt gcgcacgtat tccgtcggca
     6001 cacgggtgtt ggcatcgacc accacggaga tgtagggcgt atagccgttg tccacgcacc
     6061 actcgtacag ggcgcgcatc aaatagggct tggtggaagt gggttccgtc acgctgcatg
     6121 catccgaatc aagaccgggc agtttaaacc cttgcgcgcg cgaatgtcag gccgttttgc
     6181 atgctgtgac aggagatcgg agcgaatcgg agtgagggtg ctcgcgcagc acgctgcgcg
     6241 agctaagggc cggtcccatt gcgc
//
LOCUS       DATGOU010000096        13951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3834922, whole genome shotgun sequence.
ACCESSION   DATGOU010000096 DATGOU010000000
VERSION     DATGOU010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13951
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3834922"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /gene="rsmH"
                     /locus_tag="VKZ48_05815"
     CDS             complement(<1..665)
                     /gene="rsmH"
                     /locus_tag="VKZ48_05815"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HLU77401.1"
                     /translation="MSVHRTVLLKEAVEALAIRPDGVYVDGTFGRGGHSREILRRLGP
                     QGRLLALDRDADAVRAAQAIDDERFAIRHARFGELAQVLGAEGIATVDGVLLDLGVSS
                     PQLDEAERGFSFRADAPLDMRMDRTRGETAAEWLARVEEAELKGVIKTYGEERFAGPI
                     AKAIVAARSTGGIRTTGELARVVAAAVRTREPGQDPATRTFQAIRIHLNQELEELSLA
                     LPQA"
     gene            complement(676..1119)
                     /gene="mraZ"
                     /locus_tag="VKZ48_05820"
     CDS             complement(676..1119)
                     /gene="mraZ"
                     /locus_tag="VKZ48_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HLU77402.1"
                     /translation="MFQGASELSLDAKGRIAVPTKHRDPLVSGTGKVVLTGHPDRCLL
                     LFPSEAFRPISERISKMPTMEANARWWRRMVIGYAEELELDGSGRILISPALRKLADI
                     QKQVMLVGQGTHFEMWGLERWEAQLGAAMDEVASTPPSGTEDFVL"
     gene            1559..1741
                     /locus_tag="VKZ48_05825"
     CDS             1559..1741
                     /locus_tag="VKZ48_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77403.1"
                     /translation="MWKAAGITFGLGMALVVFEWLMARRKKEGITFTDRQRMLGILRI
                     SLVLAALVGWIAWMSE"
     gene            1809..2255
                     /locus_tag="VKZ48_05830"
     CDS             1809..2255
                     /locus_tag="VKZ48_05830"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HLU77404.1"
                     /translation="MTQSAVSRVRIPFVEHLGIRLTEQSKERAVIALDRRPELLNSWG
                     STHGGVIMTMLDYVMGAAVRGHYGADNSVLTVDLSLGFMKPATSEQMIAEGRVLHRGR
                     STAFCEAEARDQSGTLLAKAIGTFRLLPPDPNRQARSEKRESQESA"
     gene            complement(2512..3033)
                     /locus_tag="VKZ48_05835"
     CDS             complement(2512..3033)
                     /locus_tag="VKZ48_05835"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HLU77405.1"
                     /translation="MRSITRFLIFAGAAVALALGGCATTDQAQQTEVSASAPASKKKM
                     RARRAANASAVAGYKQQVAKRIYARNPQTFKGKLPPLLKSVVVLRVTVDGNGKATKVS
                     VRRSNGFKQLERVALDSVRKAGPLPRPSAEVLAGGDSFSYLETWLFREDGKFQIRSLA
                     EAQMGAEGVVARR"
     gene            3196..3711
                     /locus_tag="VKZ48_05840"
     CDS             3196..3711
                     /locus_tag="VKZ48_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77406.1"
                     /translation="MMTIMELMEFGSYVVTVVGLPFAIYAYLREQRKERENEDEEAWQ
                     QLADAYNEFLTLVLANPDLKLRTAERVAHLSEEQRERQRVIFEMLISLFERAYILLYA
                     EDLTGRHRRRWHSWDDYMHEWCRRQDFREALTDMLRGEDVEFAAYIERIAAEERAKAA
                     THQSTEQAPPQ"
     gene            complement(3737..4066)
                     /locus_tag="VKZ48_05845"
     CDS             complement(3737..4066)
                     /locus_tag="VKZ48_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77407.1"
                     /translation="MKRTMVLGVAVAMLGGCSGWWPFSGPEEQSRIPRDAVVYACEGG
                     KTLPLRVDEGGKSVMVIYPEREFRLDQRDGGRYGNGVTTLHLDDADTWLEERGNRVYQ
                     GCKRPAG"
     gene            complement(4079..4933)
                     /locus_tag="VKZ48_05850"
     CDS             complement(4079..4933)
                     /locus_tag="VKZ48_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HLU77408.1"
                     /translation="MKLITWNVQWCRGLDGRVDPQRIVEHAQRLADFDVLCLQEVAAN
                     FDDLPGSQGEDQFGRFAELLPGFTAIPGVAVDLPREGGGRQLFGNLILSRYPVLAVAR
                     TQLPSPPERRRPSMPRMLLDVRLDTPLGLVRVMTTHLEYYSPRQRAAQVEAIRAHHAE
                     ACAWAAIPARPGKQMGPFRHVPQTAAAILTADFNFGPDDALHDRLQAPNEKASRFVDV
                     WQHRYPGVPHPLTLGVHDRKRWPTPLSCDFIFATGELLPSLREVKVDGETQASDHQPV
                     LAVFDRNR"
     gene            complement(4930..6450)
                     /gene="glpD"
                     /locus_tag="VKZ48_05855"
     CDS             complement(4930..6450)
                     /gene="glpD"
                     /locus_tag="VKZ48_05855"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HLU77409.1"
                     /translation="MSEAHYDLAVIGGGINGAGIARDASGRGLRVVLLEQNDFAGGTS
                     SASTKLIHGGLRYLEHYEFRLVAESLREREVLLRLAPHLVTPLRFVLPHVKSLRPAWM
                     MRMGLWLYDRLGGRGSLPASTAVSLDGTPEGAPLRPLLRKGFVYWDAWVDDARLVIAN
                     LRSAAGYGATLMRSTRFVAARREAGVWRIEARHRDGSVRILRARALVNAAGPWVAEAA
                     HGIEGSQDMAARVQLVKGSHIVVPRVHPGRYAYLLQNDDRRIVFVLPYEERYSLIGTT
                     DVLIETPEQGAHIDAAEVRYLLAAANRYLATHLRERNIVWTYSGVRPLYDDGSANPSA
                     TTRDYHLVLDAGEDVPLLSIYGGKITTYRKLAEAALAQLRPWLEPARGEWTAHEPLPG
                     GAGASERALSTLQKQYEGLPASLVERLYRRHGSETSALLAGAVTVDDLGHHFGADLYE
                     REVRHFIAHEWAEEPDDVLWRRTKAGLHLSAAERQEFEGWFVAQQGRMQRETRCSA"
     gene            6641..7042
                     /locus_tag="VKZ48_05860"
     CDS             6641..7042
                     /locus_tag="VKZ48_05860"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HLU77410.1"
                     /translation="MRFIQAAAFALSLLFIHASYAAPDREGCQTGTPEGEEACLRPDL
                     QGVEAALDRQVHQLRRQLSKSPRTQGTRAPMRLDASQQNWLQYRLNFCNLEGLASAGT
                     GEWLQVRILECRMRMTKDRLESLRDIEAALE"
     gene            complement(7061..8224)
                     /gene="clsB"
                     /locus_tag="VKZ48_05865"
     CDS             complement(7061..8224)
                     /gene="clsB"
                     /locus_tag="VKZ48_05865"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF008427.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HLU77411.1"
                     /translation="MTSNDYLTGNRLKLLRNGDEYFPALHSAIASARRYVHLETYIFA
                     DDDAGRFIARALMDAAASGVSVRVLVDSFGTKRYFGGLRDRLLDAGVELLMYRPDLTP
                     WDIRRSRLRRLHRKIAVIDGSVGFVGGINIIDDMNTPGHTPPRLDYAVRVEGPLLELL
                     AAEADQLWRIVAWSNFRQRWMSDVAHSGDFSHKGTQRAALLVRDNLHHRSDIEDAYLG
                     AIEAAREEIILASAYFFPGVRFRHALTEAAGRGVRVVLLLQGRVEYALLHYASRALYG
                     ALLDANVEIVEYNKSFLHAKVAVIDGRWATVGSSNIDPFSLLLAREANVAVDDAVFAR
                     ELRDSLMALIRDGARPVERERWHKQPLGTRIRIWIAYGISRFLIGWFGYGRRH"
     gene            complement(8211..8978)
                     /locus_tag="VKZ48_05870"
     CDS             complement(8211..8978)
                     /locus_tag="VKZ48_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HLU77412.1"
                     /translation="MSLRVATYNIHKGFSQFNRRMVLHELRERLRETKADIVFLQEVQ
                     GQHALHANRFTNWPAEPQYEFLADSMWAEHAYGRNAVYDHGHHGNAILSRYPIISVEN
                     QDISTNRFESRGLLHCEIHVPRLDIKVHCLCVHFALIEEGRRRQLQALIDRIRENVPA
                     DAPVIVAGDFNDWRNRVGLRLHEAVGLQEVFRVHHGRSARSYPSRLPLFCLDRIYVRG
                     FRIAHAEVHHGQVWSRISDHAMLSAQLAFMSERRDQQ"
     gene            complement(9025..9507)
                     /gene="nudB"
                     /locus_tag="VKZ48_05875"
     CDS             complement(9025..9507)
                     /gene="nudB"
                     /locus_tag="VKZ48_05875"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007579771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HLU77413.1"
                     /translation="MSETYKIPRSVLVVIHTPDLQVLLMERVPWPGFWQSVTGSVDYE
                     DEPYRETAVREVREETGLDATRYDLTDWGVENDFEIFKRWRSRYAPGVTHNRERVFGL
                     LVPEPMPIRCAPDEHYGYVWLPWKEAAEKCFSWSNRDAILSLPERVGQAAAAEGGSRR
                     "
     gene            complement(9504..9941)
                     /locus_tag="VKZ48_05880"
     CDS             complement(9504..9941)
                     /locus_tag="VKZ48_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HLU77414.1"
                     /translation="MDYIDLLTNPGEAGVFATPRDVRALDDALDRAGFERWRVDLQGV
                     SGKPELMRVFQRALGFADTFGANWDALHDALSERALDEPAGVALTLAHCGDVARAQGG
                     HFERLVEVLDAVAENCYDEDIPFWVFIDGVAAADFDLPRLDEK"
     gene            complement(9948..10340)
                     /locus_tag="VKZ48_05885"
     CDS             complement(9948..10340)
                     /locus_tag="VKZ48_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HLU77415.1"
                     /translation="MTRTLRTLLFTLLWLVCAASHAAADLPVVPLHELPPEARETLVL
                     IRQGGPFPYRRDGTVFRNFERRLPSRPRGYYREYTVRTPGLSHRGARRIVAGAGPDRD
                     VRTSGEYYYTDDHYDSFRRIGMPSDDRR"
     gene            complement(10359..12140)
                     /gene="aspS"
                     /locus_tag="VKZ48_05890"
     CDS             complement(10359..12140)
                     /gene="aspS"
                     /locus_tag="VKZ48_05890"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HLU77416.1"
                     /translation="MRSHYCGQVDTGLLDQQVSLCGWVHRRRDHGGVIFIDLRDREGL
                     VQVVFDPDRADAFQIAESVRNEYCLRVVGKVRRRPEGTTNPNIRSGEIEVLGHEIEIL
                     NPSVTPPFQLDDDNLSETVRLTHRVLDLRRPQMQHNLMLRYRVAMAARKYLDEHGFID
                     IETPMLTKSTPEGARDYLVPSRVHDGEFFALPQSPQLFKQMLMVAGYDRYYQITKCFR
                     DEDLRADRQPEFTQIDCETSFLTEEQIRELFEGLIREVFKQALDVDLPNPFPIMTYAD
                     AMRDYGSDKPDLRVPLKFTELTDVMKTVDFKVFRAAADLKDGRVAALRIPNGGTLTRK
                     EIDEYTAYVAIYGAKGLAYIKVNDASKPSEEGLQSPIVKFLPPEALKTILERTGAQNG
                     DLIFFGADRAKIVNDALGALRVKLGHEKGYAEEGWRPLWVVDFPMFEYDEDEKRWAAM
                     HHPFTSPKDGHEDYLESDPSKAQAKAYDMVLNGWEIGGGSVRIHREEVQSKVFRALGI
                     GEEEARAKFGFLLDALQYGAPPHGGIAFGLDRIVTMMAGAESIRDVIAFPKTQRAQCL
                     LTQAPSPVDEKQLRELHIRLRNPEKKG"
     gene            complement(12153..12794)
                     /locus_tag="VKZ48_05895"
     CDS             complement(12153..12794)
                     /locus_tag="VKZ48_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HLU77417.1"
                     /translation="MKRYFITGLLFLVPLVITVWVLSIVIATMDRSLDLLPAALHPEV
                     WFGRNIPGLGALLTLLVILFTGMFASNILGERLLRIWESALLRVPVVNSIYNGVKQIS
                     DTVFKPGGQAFRKALLVRWPHGDAWTIAFLTGTPGGDVVNYLDGDYVSVYVPTTPNPT
                     SGYFIMLRCTEVIELDMSIDEALKYIISMGVVSPRDHPKKEAVAAEEPSSLQS"
     gene            complement(12942..13160)
                     /locus_tag="VKZ48_05900"
                     /pseudo
     CDS             complement(12942..13160)
                     /locus_tag="VKZ48_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486780.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
     gene            complement(13383..13838)
                     /gene="ssb"
                     /locus_tag="VKZ48_05905"
     CDS             complement(13383..13838)
                     /gene="ssb"
                     /locus_tag="VKZ48_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HLU77418.1"
                     /translation="MASVNKVILVGNLGADPETRYLPSGDAITNIRIATTDRWKDRQS
                     GEMKEATEWHRVAFFGRLAEIAGQYLKKGSPVYVEGRIRTRKWQDKETGQDRYSTEIV
                     ADNMQLLGSRQGMGDAPAGDFGGGGSRAPAKKAPAAGGGFGDMDDDIPF"
BASE COUNT         2557 a         4602 c         4313 g         2479 t
ORIGIN      
        1 gcttgcggca gcgctagcga caactcctca agctcctgat tgaggtgaat ccgtatagcc
       61 tggaacgtgc gcgtcgccgg gtcctgcccg ggttcacgcg tgcggacggc ggcagccacg
      121 acacgggcaa gctctcctgt cgtgcgtatc ccgcctgtgg accgagcagc aacaatcgcc
      181 tttgcaatcg ggccagcaaa ccgttcttcg ccataggtct tgatcactcc cttcaactcg
      241 gcttcttcca cacgcgccag ccattcggct gccgtctctc cccgcgtacg atccatccgc
      301 atgtccagcg gcgcatcggc acgaaagctg aacccccgct ccgcctcatc cagttgcggc
      361 gaagacacgc ccaaatcgag cagcaccccg tccaccgtcg cgatgccttc cgcaccgagc
      421 acctgcgcaa gttcaccgaa acgcgcatgg cggatggcga agcgttcgtc gtcgatcgcc
      481 tgcgcggcgc gcacggcgtc ggcgtcacgg tcgagcgcga gcagacgccc ttgtgggcca
      541 agccggcgca agatttcccg gctgtgtccg ccccggccga acgtgccgtc cacatagacg
      601 ccgtcgggcc gaatcgctaa cgcttcgacc gcctccttca gcaggacggt ccgatgtacg
      661 ctcacatgcc acccgctaaa gaacgaaatc ttcggtaccg gacggcgggg tcgaagccac
      721 ctcatccatc gccgcgccca attgcgcttc ccagcgctcc aggccccaca tttcgaaatg
      781 ggtgccctga ccgaccagca tcacctgttt ctggatgtcg gccagcttgc gcagtgcggg
      841 ggaaatgagg atgcggcccg agccgtcgag ttcgagttct tcggcgtagc cgatgaccat
      901 gcggcgccac cagcgggcgt tcgcctccat ggtcggcatt ttggaaatcc gttcgctgat
      961 cggcctgaaa gcctcgctgg ggaagagcag caggcagcgg tccgggtggc cggtcagtac
     1021 gaccttgcct gtcccggaca ccagcgggtc gcgatgcttg gtcgggacgg caatgcgccc
     1081 cttcgcatcg agactcagct cgctcgctcc ctgaaacacg gtgtctcctc catccgggag
     1141 aaagcagcct tatgccactt tcctccacga attcccactt ctcgccactc tagagtagaa
     1201 aaaatggagg gtcaagcgcc caatgcaaat ttttccgtgc tgtgacaatg acttagcggc
     1261 aactcttgag gcaattcgtg cgcgcgaata gccctttaga aatgaagggg atagcattca
     1321 aaccgaaagt gatttgtgag aagcaggcgc gagaaacggc cgcttgggcg gacgagactg
     1381 tagggcaagg acgacgcctt atttgttcgt ccggcgcgga gtctttacgg tcgacgcctt
     1441 tcagcgggcg ctgagggtga cgcgacgcac gggccgctgc cgcagccagc acgcggtata
     1501 tgcccgggcc gaattcgagg aagccctcta gaatccgccc gctggaggat ttggaatcat
     1561 gtggaaagca gcgggcatta ccttcggtct gggcatggcg ctggtcgtgt tcgagtggct
     1621 gatggcgcga cgcaagaaag aagggatcac ctttaccgat cggcagcgca tgctgggcat
     1681 tctgcgcatc agcctcgtac tggcggcact ggtcggctgg atcgcatgga tgtcggaatg
     1741 agtcgtcgta acggcggcga ggcccatgac gttgaaagca cgacgcggct catgccatca
     1801 tcggacgcat gactcaatct gctgtttccc gcgtccgcat tccgttcgtc gaacatctcg
     1861 gcatccgtct caccgaacaa tcgaaggaac gcgccgtcat cgcgctcgat cggcgtcccg
     1921 aactgctcaa cagttggggc tccacgcacg gcggcgtgat catgaccatg ctggactatg
     1981 tcatgggcgc ggccgtgcgc ggccactacg gtgccgacaa cagtgtgctc acggtcgatc
     2041 tgtcgttggg cttcatgaag ccggccacct ccgaacagat gatcgcggaa gggcgcgtgc
     2101 tgcatcgcgg ccggtccacg gctttttgcg aagccgaggc acgcgaccaa agcggcacat
     2161 tgctcgccaa ggcgatcggt acgttccggc tgctgccgcc cgaccccaac cggcaggcgc
     2221 ggtcggaaaa gcgcgaatcg caggaaagcg cctaaaggcg agaagctgaa aagcgaaaaa
     2281 gcctggaaca cagagcgaac agagaaccac ggagaactcg gagagaagca cagctaggtt
     2341 ttcctctgtg cgctctgtgt cctctgtggt tcaaggtttt tttgaagggt cgctacgcct
     2401 ccgcccaggt tcgcgacggc gtagttcgct gcacggcgga agcgctaact cagtgaccgc
     2461 cgtgctctcc gtgtttcagg ctttgaacca cgctgcgcat cgcgcaggcg ctcaacgacg
     2521 cgcgaccacg ccttccgcgc ccatctgcgc ttcggccaga ctgcggatct ggaatttacc
     2581 gtcctcgcgg aaaagccagg tctccagata gctgaacgaa tcgccgcccg cgagcacttc
     2641 cgctgaagga cggggtagcg gaccggcttt gcgcacgctg tccagcgcga cccgctccag
     2701 ttgcttgaag ccgttggagc ggcgcaccga caccttcgtc gccttgccgt tcccatccac
     2761 cgtgacccgt aacaccacca ccgatttgag cagcggcggc agcttgcctt tgaaggtctg
     2821 cgggttgcgc gcataaatgc gcttcgccac ctgctgttta tagccggcga cggccgacgc
     2881 attcgccgcc ctgcgcgcgc gcattttctt tttcgacgcc ggcgcgctgg cggacacctc
     2941 cgtctgctgt gcctggtcgg tcgtggcgca accgcccagt gccagcgcaa cggctgcacc
     3001 ggcaaagatc aaaaaccggg tgatggatcg catgtgaagc tccttccttg atactcgccg
     3061 caggctagca cgttcacgcg tcatgctatt gtcatagacg tgaactgctt cacgcaacgc
     3121 ggaacacaag cgcaacaaag cgtcgtgcgt tgcacacagg cacggcgctg tgccaccctc
     3181 gcgccgaagg gagcgatgat gaccatcatg gaattaatgg agttcggcag ttacgtcgtg
     3241 accgtcgtcg gcctgccgtt cgcgatctac gcctacctgc gcgaacagcg gaaggagcgc
     3301 gaaaacgaag acgaggaagc ctggcagcaa ctggcggacg cctacaacga gttcctcacg
     3361 ctcgtgctgg ccaatccgga tctgaaattg cgcaccgcgg aacgcgtcgc ccacttgagc
     3421 gaagaacagc gcgaacggca gcgggtgatc ttcgaaatgc tcatttcgct gttcgagcgc
     3481 gcctacattt tgctctatgc cgaggatctg accggacgcc accgccggcg ctggcattcc
     3541 tgggacgact atatgcacga gtggtgccga cggcaggatt tccgcgaagc gctgaccgac
     3601 atgctgcgcg gcgaagacgt cgaattcgcc gcctatatcg agcgcattgc ggcggaggaa
     3661 cgcgccaagg cggcgacgca ccaaagcacc gagcaggcgc caccgcaata acgcgcgcgg
     3721 cgtcggccca ccgcgctcaa ccggctggac gcttgcagcc ctgataaacg cgattgccgc
     3781 gttcttccag ccaggtgtcg gcgtcgtcga gatggagggt cgtcacgccg ttgccgtaac
     3841 ggccgccgtc gcgctgatcg agacggaact cccgctccgg atagatcacc atgaccgatt
     3901 tgccgccctc gtccacgcgc agcggcagcg tcttgccgcc ctcgcaggca tacaccaccg
     3961 catcgcgcgg aatgcgcgac tgttcctcag gcccgctgaa cggccaccag ccgctgcagc
     4021 ctcccagcat cgccacggcg acgcccagca ccatcgttct tttcattttc gctcctgatt
     4081 atcgattccg gtcgaacacc gccaataccg gctgatgatc cgacgcctgc gtctcgccgt
     4141 ccaccttcac ctcgcgcagc gacggcagca gctccccggt ggcgaaaatg aaatcacaac
     4201 tcaagggcgt cggccatcgt ttgcgatcgt gcacgcccag cgtgagcgga tgcggcacgc
     4261 ccggatagcg atgctgccac acatcgacga agcggctggc cttttcattc ggcgcctgca
     4321 agcgatcgtg cagcgcatcg tccggcccga agttgaaatc ggctgtgaga attgccgcgg
     4381 cggtttgcgg cacatggcgg aagggtccca tctgcttgcc cgggcgcgcc ggtatcgcgg
     4441 cccacgcgca ggcctcggcg tgatgcgcac gaatggcctc cacctgtgcg gcgcgctgcc
     4501 ggggcgagta gtactccaga tgcgtggtca tcacccgcac cagtccgagc ggcgtgtcca
     4561 gccggacatc gagcagcatg cgcggcatgc tcgggcggcg gcgctcgggc ggcgacggca
     4621 gttgcgtgcg cgccaccgca agcaccggat aacggctcag gatgagattg ccgaacaact
     4681 gtcggccgcc gccttcgcgc gggagatcta ccgccacgcc gggaatggcc gtaaagccgg
     4741 gcagcaactc ggcgaatcgt ccgaactggt cttcgccctg gctgcccggc aggtcgtcga
     4801 aattcgccgc cacttcctgc aggcacagca cgtcgaaatc ggcgagccgt tgcgcgtgtt
     4861 cgacgatacg ctgcggatcg acgcgaccgt ccagaccgcg gcaccactgc acgttccagg
     4921 tgatcagctt catgcgctac accgtgtttc ccgctgcata cgaccctgct gcgccacaaa
     4981 ccacccctcg aactcctgcc gctcggccgc gctcaaatgc aaccccgcct tcgtacgtcg
     5041 ccacaacacg tcgtccggtt cttccgccca ttcatgagcg atgaaatggc ggacctcgcg
     5101 ctcgtaaaga tcggcgccga aatggtggcc gagatcgtcc acggtgacgg cgcccgcaag
     5161 caatgcgctc gtctcgctgc cgtggcgccg gtaaagccgt tcgaccagcg acgcgggcaa
     5221 gccttcgtat tgcttctgca gcgtggatag cgcgcgctcg gatgcgccag cgccgccggg
     5281 caacggctca tgcgccgtcc attcgccgcg cgccggctcg agccacggcc gcaactgcgc
     5341 aagcgccgct tcggccagct tacgataggt ggtgatcttg ccgccgtaaa tgctcagcaa
     5401 cggcacgtcc tcgccggcat cgaggacgag gtgataatcc cgtgtcgtcg ccgaagggtt
     5461 ggccgagccg tcgtcgtaca gtggccgcac gccgctgtag gtccagacga tgttgcgctc
     5521 gcgcaagtgc gtcgcgagat agcggttcgc cgcggcgagc agatagcgca cctccgccgc
     5581 atcgatgtgc gcgccctgct ccggcgtttc gatcagcacg tccgtcgtgc cgatcagact
     5641 gtagcgctcc tcataaggca ggacgaacac gatgcgccgg tcgtcgttct gcaacagata
     5701 ggcgtaccgg cccggatgca cgcgcggcac gacgatgtga ctgcctttca cgagttgcac
     5761 ccgcgcagcc atgtcctgcg atccttcgat gccgtgcgcc gcttcggcca cccatgggcc
     5821 ggcggcgttc accagcgcgc gtgcgcgcag gatccgcacg ctgccgtcgc gatgacgcgc
     5881 ctcgatgcgc cagacgccgg cctctcgtcg cgcggcgacg aaacgcgtcg aacgcatgag
     5941 cgttgcgccg tagccggccg cggagcgcag attggcaatc accagacgcg cgtcatccac
     6001 ccaggcgtcc cagtaaacga agcccttgcg cagcagcgga cgcaacggcg cgccttcggg
     6061 cgtgccgtcc agcgacacgg cggtcgacgc cggcaggctg ccgcgcccgc cgagacggtc
     6121 gtagagccac aggcccatgc gcatcatcca cgccgggcgc aacgacttca catgcggcag
     6181 gacgaagcgt agcggcgtca ccagatgcgg cgccagccgc aacaggactt cgcgctcccg
     6241 cagcgattcc gccaccagcc ggaattcgta atgctccaga taacgcaatc cgccgtggat
     6301 gagcttggtg ctcgcggacg aggtgccgcc ggcaaaatca ttctgttcca aaagcacgac
     6361 acgtaagccc cgtccgctcg cgtcccgcgc gatgcccgcg ccgttgatgc cgccgccgat
     6421 gacggcaaga tcgtagtgtg cttccgacat tgaaattccc gaatcgcacc gggcgtcgcg
     6481 acgtgtatcc atcgcccgcg cgttcacccg aaactaaccg gtgcgggaat tgcccgcaat
     6541 cctgtcgccc gcctcctgcc atccgtataa tggcgcgcgg cacgaaactt ccttgttgca
     6601 cgactgccgc cttccaacgc acgtccaatt cccgtacctg atgagattca ttcaagcagc
     6661 cgccttcgcc ttgagcctcc tgttcataca tgcctcctac gcggcaccgg atcgcgaagg
     6721 ctgtcagacc ggtaccccgg agggcgagga agcctgtctg cggcccgatc tgcaaggcgt
     6781 ggaagcggca ctcgaccgcc aggtgcatca actgcgtcgc cagttgagca agtcgccgcg
     6841 cacgcagggc acgcgcgcac cgatgcggct ggatgcctcg cagcagaact ggctgcaata
     6901 ccgcctcaac ttctgcaacc tcgagggcct ggccagtgcg ggcaccggcg agtggctgca
     6961 ggtgcgcatc ctcgaatgcc gtatgcgcat gaccaaggac cggctcgagt ccctgcgcga
     7021 catcgaagcg gcgctggaat aggacgcgcc ggcggcgcgc tcaatggcgc cggccgtacc
     7081 cgaaccagcc gatcagaaaa cgtgagatgc cgtaggcgat ccagatgcgg attcgcgtgc
     7141 ccagcggctg cttgtgccaa cgctcgcgct cgaccggccg tgcgccgtcg cggatcaacg
     7201 ccatcagact gtcgcgcagt tcgcgggcga acacggcatc gtccaccgcc acattggctt
     7261 cgcgcgcgag cagcaggctg aagggatcga tgttcgacga acccaccgtc gcccagcgtc
     7321 cgtcgatgac cgcaaccttg gcgtgcagga agctcttgtt gtactccacg atctccacat
     7381 tggcgtcgag cagggcgccg tagagcgcac gcgatgcgta atgcagcagg gcatattcga
     7441 cgcgcccctg cagcagcagc acgacgcgca cgccccggcc ggcggcttcg gtcaaggcgt
     7501 gacgaaagcg cacacccgga aaaaaatacg cgctcgccag aatgatctcc tcgcgcgccg
     7561 cctcaatcgc acccagatag gcatcttcga tgtccgagcg gtggtgcagg ttgtcgcgca
     7621 ccagcagcgc cgcacgctgc gtgcccttgt ggctgaaatc gccgctgtgc gccacatcgc
     7681 tcatccagcg ctggcggaag ttcgaccagg cgacgatgcg ccacaactgg tcggcttccg
     7741 ccgcaagcaa ttcgagcagc ggcccttcca cccgcactgc atagtccaga cgcggcggcg
     7801 tgtgccccgg cgtattcatg tcgtcgatga tgttgatgcc gccgacgaat cccacactgc
     7861 cgtcgatcac cgcaatcttg cgatgcagtc ggcgcagacg cgaacggcgg atatcccacg
     7921 gcgtcaggtc gggccggtac atcagcagtt ccacgcccgc gtcgagcagg cgatctcgca
     7981 ggccgccgaa atagcgcttg gtgccgaagc tgtcgaccag caccctcacg ctgacgccgc
     8041 tggcagcggc gtccatcagc gcgcgcgcaa tgaaacggcc ggcatcgtcg tcggcgaaga
     8101 tgtaggtttc cagatgcaca tagcgtcgcg cggacgcgat cgccgaatgc agcgcgggga
     8161 aatactcgtc cccgttacgc agcagcttca ggcgattgcc cgtcaggtag tcattgctgg
     8221 tcacggcgct ctgacatgaa cgcgagttgg gcgctcagca tcgcgtggtc ggagatgcgc
     8281 gaccagacct gaccgtgatg gacctcggca tgcgcgatgc gaaagccgcg aacgtagatg
     8341 cgatccaggc agaacagcgg caggcggctg gggtagctgc gcgccgaacg cccatgatgc
     8401 acgcggaaca cttcctgcaa cccgaccgct tcatgcaatc ggagtccgac ccggttgcgc
     8461 cagtcgttga agtcgccggc cacgatgacc ggcgcgtcgg ccggtacgtt ttccctaatg
     8521 cggtcgatca gcgcctggag ctgacgtcgc cgcccttcct cgatcagcgc gaaatgcacg
     8581 caaaggcagt gcaccttgat atccagccgc ggcacatgga tttcgcaatg cagcagcccg
     8641 cggctttcga aacgattggt ggagatgtcc tggttctcca ccgagatgat gggatagcgg
     8701 gacaggatgg cgttgccgtg gtgcccatgg tcgtacacgg cgttgcggcc gtaggcgtgc
     8761 tcggcccaca tgctgtcggc caggaattcg tactgcggct cggccggcca gttggtgaag
     8821 cgattggcat gcaaagcatg ctgcccctgc acttcctgca gaaacacgat gtcggccttc
     8881 gtttcgcgca accgctcgcg cagctcgtgc aacaccatgc ggcgattgaa ttgtgaaaag
     8941 cccttatgga tgttgtaggt cgcgacgcgc aaactcatga tgacctgacg gaatccttcg
     9001 ctgttccaac ggcaacgcgg cccatcatcg tcgcgagcct ccttccgcgg ccgcagcctg
     9061 cccgacgcgc tccggcaggg agagaatggc gtcgcgatta ctccatgaaa aacacttctc
     9121 cgccgcttcc ttccacggca gccagacata accgtaatgc tcgtcgggcg cgcagcggat
     9181 gggcatcggc tcgggcacca gcaggccgaa tacccgctcg cgattatggg tcacgcccgg
     9241 cgcataacgg ctccgccagc gcttgaaaat ctcgaaatcg ttttccacgc cccagtcggt
     9301 cagatcgtag cgcgtggcgt ccagccccgt ctcctcgcgc acctcgcgga ccgccgtctc
     9361 gcggtagggt tcgtcttcgt aatctacgct cccggtcacc gattgccaga aaccgggcca
     9421 gggtacccgc tccatcagca gcacctgcag gtcgggcgtg tggatgacca ccagcaccga
     9481 acgcgggatc ttgtaggttt cgctcatttc tcatccagcc gcggcagatc gaaatccgcc
     9541 gccgcgacgc cgtctatgaa cacccagaac gggatgtctt cgtcgtagca attctccgcc
     9601 acggcgtcga gcacctccac cagccgctcg aagtggccgc cctgcgcgcg cgccacatcg
     9661 ccgcaatgcg ccagggtcag ggcgaccccg gccggttcgt cgagcgcacg ctcgctcagc
     9721 gcgtcgtgca acgcgtccca gttcgcgccg aaggtgtcgg caaagccaag cgcgcgctgg
     9781 aagacgcgca tcagttccgg tttgccgctc acgccttgga ggtccacccg ccagcgctcg
     9841 aacccggcgc ggtcgagcgc gtcgtccagc gcacggacgt cccgcggcgt ggcaaacacc
     9901 cccgcttcgc cgggattggt cagcaggtcg atgtaatcca tcggcgctca cctcctgtcg
     9961 tccgacggca tcccgatgcg ccggaacgaa tcgtaatgat cgtcggtgta atagtactcg
    10021 ccgctggtac gcacatccct gtccgggccg gcgccggcga cgatccgccg cgcgccgcga
    10081 tgactgaggc ccggcgtgcg gaccgtgtac tcacggtaat agccgcgcgg ccggctgggc
    10141 aaccgccgct cgaaattgcg gaacaccgtg ccgtcgcggc ggtaggggaa cggaccgccc
    10201 tgccggatga gcacgagcgt ctcgcgcgct tcgggcggca gctcgtgcag cggcacgacg
    10261 ggcagatccg ccgcggcgtg cgaggccgcg cagaccagcc acaacagcgt gaacagcagg
    10321 gtccgcagcg tgcgcgtcat ggtcgcggac ccgatccgtc agcccttctt ttcgggattg
    10381 cgcagccgga tatgcagctc gcgcaattgc ttctcgtcca cgggactggg cgcctgcgtc
    10441 agcagacact gcgcacgctg ggtcttgggg aaggcgatca cgtcgcggat cgattcggcg
    10501 cccgccatca tggtgacgat gcgatccaga ccgaaggcga tgccgccatg gggcggcgcg
    10561 ccgtactgca gcgcatcgag caggaagccg aatttcgccc gcgcttcctc ttcgccgatg
    10621 cccagggcac gaaagacctt cgactgcact tcctcgcggt ggatacgcac cgagccgccg
    10681 ccgatctccc agccgttcag caccatgtcg taagccttgg cctgcgcctt ggatggatcg
    10741 gactcgagat aatcctcgtg tccgtccttg ggcgaggtga agggatggtg catggccgcc
    10801 cagcgcttct cgtcctcgtc gtattcgaac atggggaagt ccacgaccca cagcgggcgc
    10861 cagccttcct ccgcataacc cttctcgtgg ccgagcttga cgcgcaaggc cccgagtgcg
    10921 tcattgacga ttttggcgcg gtcggcgccg aagaagatca ggtcgccgtt ctgcgcaccg
    10981 gtgcgctcca gtatggtctt cagtgcctcg ggcggcagga acttcacgat gggcgactgc
    11041 agcccttcct cgctcggctt cgaggcgtcg ttgactttga tgtacgcaag acccttcgcg
    11101 ccgtagatgg cgacgtaggc ggtgtactcg tcgatttcct tgcgcgtcag cgtgccgccg
    11161 ttgggaatgc gcagcgcggc cacgcgaccg tccttgagat cggccgcggc gcggaatact
    11221 ttgaagtcca ccgtcttcat tacgtcggtc agttcggtga acttgagcgg tacgcgcagg
    11281 tccggcttgt cggagccgta gtcgcgcatg gcgtcggcgt aggtcatgat cgggaagggg
    11341 ttcggcaagt cgacgtcgag cgcctgcttg aacacctcgc ggatcaaccc ttcgaacaac
    11401 tcgcgaatct gctcctcggt caggaacgag gtctcgcagt cgatctgcgt gaattccggc
    11461 tgccggtcgg cgcgcaggtc ctcgtcgcgg aagcatttgg tgatctggta gtagcggtcg
    11521 taaccggcca ccatgagcat ctgtttgaac aactgcggcg actgcggcag cgcgaaaaac
    11581 tcgccgtcgt gcacgcgcga gggcacgagg tagtcgcgcg cgccttcggg tgtcgacttg
    11641 gtgagcatcg gcgtttcgat gtcgatgaaa ccgtgctcgt cgagatactt gcgcgcggcc
    11701 atggcgacgc ggtaacgcag catcaggttg tgctgcatct gcggacggcg cagatcgagc
    11761 acgcgatgcg tcaaccgcac tgtttccgag aggttgtcgt cgtccagttg gaagggcggc
    11821 gtgaccgaag ggttgagaat ttcgatctcg tggcccaaca cctcgatttc gccggagcgg
    11881 atgttggggt tggtcgtgcc ttcggggcgg cgacgcacct tgcccaccac acgcaggcag
    11941 tactcgttgc gcacgctttc ggcaatctgg aaggcatcgg cgcgatcggg gtcgaacacc
    12001 acctgcacca ggccttcgcg gtcgcgcagg tcgatgaaga tgacgcctcc gtggtcgcga
    12061 cgccgatgca cccagccgca caggctgact tgttgatcga gtaggcccgt atcgacctgt
    12121 ccgcaataat gactacgcat gaagtttcgt tcttaagatt gcaaggaaga cggctcttcg
    12181 gccgccacgg cttctttctt gggatgatcg cgcggcgaca ccacgcccat ggaaataatg
    12241 tacttcagcg cctcgtcgat cgacatatcg agttcgatga cttcagtgca tcgcagcatg
    12301 atgaaatagc cgctggtcgg gttgggtgtg gtcggcacat agacgctgac atagtcgccg
    12361 tccagataat tcaccacatc gccgccgggc gtgccggtca gaaaggcgat ggtccaggcg
    12421 tcgccatgcg gccagcgcac cagcagcgcc ttgcggaagg cctggccgcc cggcttgaac
    12481 accgtatcgc tgatctgctt gacgccgttg tagatcgagt tgaccaccgg cacccgcagc
    12541 aaggcgcttt cccagatccg cagcagccgc tcgccaagga tattggaggc gaacatgccg
    12601 gtaaacaaaa tcaccagcaa cgtcagcagc gcgcccagac cggggatatt gcgaccgaac
    12661 cacacctcgg gatgcagcgc cgcgggcagg aggtcgagcg agcggtccat ggtcgcgatg
    12721 acgatgctga ggacccacac cgtaatgacc agggggacga ggaacagcag cccggtaatg
    12781 aaatagcgct tcaaaggagg gttccgtggc gggggtgcgg cgtgggccct tgcgggcgga
    12841 gccgacccga caatggatcg gctgttcgtt ctatatcagg aatgcgtgca ggcggcgcca
    12901 caggtatgcg tcgaagcgct gtcggatttg gattcgccgc ttgaggaatc aggagcggca
    12961 ggcttggcgc cgctgccgcc gttcttgaag tcggtgacat accagcccga acccttgagt
    13021 tggaacccgg ctgcggtcag cattttgctg aatgtttcct tcttacattc gggacaggtg
    13081 gtcaacaacg gatcgctgat tttctgcaga tattccttct ggaatccgca cgatccgcaa
    13141 cggtactcat aaatcggcat gtcgatgctc cggacgaagc aaaaagtcac aaaatgctcg
    13201 gattataccg ggcagcccga cgcccacgcc gaatcgctcg cgtgaccgag gactttgtac
    13261 ggcctgcatc aaaggcgtcc gccgccttag atcggggcga cgcgtccggc tttcaagccg
    13321 cggcgccgga aacgaaaagg ccggcgctga ggccggcctt ggaatgtgcg agttgcgtcg
    13381 gactagaacg ggatatcgtc gtccatgtcg ccgaagccgc cgccggctgc cggcgccttc
    13441 ttcgccggcg cccgcgaacc gcccccgccg aaatcccccg ccggggcgtc acccatgccc
    13501 tgacggctgc cgagcagttg catgttgtcg gctacgattt cggtcgaata acggtcctga
    13561 ccggtttcct tgtcctgcca cttgcgcgtg cgaatgcggc cttccacgta caccggggag
    13621 ccttttttca gatactgacc ggcgatctcg gccaggcggc cgaagaaggc gacgcgatgc
    13681 cattccgtcg cttccttcat ttcgccgctt tgccgatcct tccagcgatc ggtggtggcg
    13741 atgcggatat tggtgatggc atcaccgctg ggcagatagc gtgtttccgg atcggcgccg
    13801 agattgccga ccaggatcac cttgttgact gaggccattg tccttttcct cctaaatagc
    13861 ggtatccatc ctcgtctcgc gcggcgtccg tgcgcggcgc gccgtgcact atagctccac
    13921 tttccccgca acggcagcgc gcgtcgcgat t
//
LOCUS       DATGOU010000097        11668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3390024, whole genome shotgun sequence.
ACCESSION   DATGOU010000097 DATGOU010000000
VERSION     DATGOU010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11668
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3390024"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1161
                     /gene="obgE"
                     /locus_tag="VKZ48_05910"
                     /pseudo
     CDS             157..1161
                     /gene="obgE"
                     /locus_tag="VKZ48_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006400418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
     gene            1167..2285
                     /gene="proB"
                     /locus_tag="VKZ48_05915"
     CDS             1167..2285
                     /gene="proB"
                     /locus_tag="VKZ48_05915"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HLU77419.1"
                     /translation="MKGAIGAARRLVVKVGSALVTHDGEGVDYEAIAHWAAEIAELRR
                     GGREVVLVSSGAVAEGLKRLGWRKRPRSMHELQAAAAVGQMGLVQAYESSFRVHGLQT
                     AQILLTHADLADRQRYLNARSTIRTLLKLGVVPVINENDTVVTDEIKFGDNDTLAALV
                     TNLIEADLLVILTDQAGLYTADPRKAPTAQLVRKAQAGDPALERMAGGAGTQISRGGM
                     ITKVRAAARAARSGAHTVIASGREPKLLSRLIAGEAIGSLLYAEHEPLAARKQWMADH
                     VQLSGRLQLDAGAARALRTEGKSLLPVGVRAVEGDFERGEVVACIDPEGREVARGLIN
                     YGAAETRRILGHSSQDIERILGYVEETELIHRDNLVLI"
     gene            complement(2296..2832)
                     /locus_tag="VKZ48_05920"
     CDS             complement(2296..2832)
                     /locus_tag="VKZ48_05920"
                     /inference="COORDINATES: protein motif:HMM:NF020331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HLU77420.1"
                     /translation="MLRLLLLCTLLATLWAHAQIPLFDRDPRGAVPEEVEIFKEEQLN
                     LPAAPEQKNLIAFDPGRPTTMKFYVDAASVSVGQDQVVRYTLVVTGDEDTRNVMYEGV
                     RCKSDERKTYAYGRRDGTWSLARDPQWEAFRYDVPRRTLYRNFLCPLRGPVQSADEAV
                     GALRNGVHPLVKQFSPYE"
     gene            complement(2920..3384)
                     /locus_tag="VKZ48_05925"
                     /pseudo
     CDS             complement(2920..3384)
                     /locus_tag="VKZ48_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020747476.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
     gene            3504..5249
                     /locus_tag="VKZ48_05930"
     CDS             3504..5249
                     /locus_tag="VKZ48_05930"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HLU77421.1"
                     /translation="MRVSQFFLGTLKEAPADAEIVSHKLMLRAGLIRRLAGGIYTWMP
                     LGLRVVRKVEAIVREEMNRAGAIELFMPAVQPAELWQESGRWQKYGPELLRVKDRHDR
                     DFCIGPTHEEVITDLVRREVKSYRQLPLNFYQIQTKFRDEIRPRFGVMRGREFTMKDA
                     YSFHADYEDLQREYANMYETYTRIFTRLGLGFRAVAADTGSIGGTGSHEFHVLADSGE
                     DAIAFCPQSDYAANVELAEALAPDTPRAAAGEALAKVPTPGKTRCEDVAALLDLPLER
                     TVKAIAVMHDNEFVLLLLRGDHELNEIKAQKVEGLDPFRFATDAEIQEHLGCKPGYIG
                     PVDIDAKIRVIADRSVAVMSDFVCGANEEGFHLRGVNFGRDLPEPAWVADIRKVLAGD
                     PSPDGEGTLEIVRGIEVGHIFQLRTNYSAKMQAVFLDQDGKQQPFEMGCYGIGVTRIV
                     GAAIEQNHDERGIIFPDPIAPFQLAIVPVGYHKSEAVRQAADRLYAGLRAAGIDVLLD
                     DREERPGVMFADMELIGIPHRVVVGERGLKDGKIEYQARRDKAAQTVELEQALPFLQD
                     RLCPSTTDDSEPRSR"
     gene            5291..5818
                     /locus_tag="VKZ48_05935"
     CDS             5291..5818
                     /locus_tag="VKZ48_05935"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HLU77422.1"
                     /translation="MYEPLSASVRSAMARSVADEGNVRLAFADPQDGYRWLVAMSKRL
                     EKRIPDAGFREELLKMVHYESTRAGLDPQLVLGIIQVESNFRKYAVSKAGARGLMQVM
                     PFWVELLGDGRHNLFHVRTNLRYGCTILRHYLDIEKGNLFRALGRYNGSLGREAYPNR
                     VMAAWRTFKYDGKLI"
     gene            complement(5829..6557)
                     /gene="pyrF"
                     /locus_tag="VKZ48_05940"
     CDS             complement(5829..6557)
                     /gene="pyrF"
                     /locus_tag="VKZ48_05940"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HLU77423.1"
                     /translation="MTQALLSAKNIPPRERLIVALDLPNIDEAQKLVETLGDSVHFYK
                     LGLELFMSGRYFELLERLVKADKKVFADLKFFDVPETVRSAVRGLRNRGVTFATVHGN
                     QAIMEAAGADKGEVKILAVTVLTSLDRGDLDDLGFDCDVEKLVLSRARRALAAGCDGV
                     ISSGLEAPMLKSELGERVMVVTPGIRPVENRPADDQKRTVDVAQAFTNGADYIVVGRP
                     IRQAADPRAAAEQIQATIAKTFAG"
     gene            6895..8403
                     /locus_tag="VKZ48_05945"
     CDS             6895..8403
                     /locus_tag="VKZ48_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77424.1"
                     /translation="MSLHLSLPPSGERVDAQPEPDPLQIAAWVDGLRNEEPLRAAQQL
                     VDFLVPMNRTRMRVALRQEVAELIVPAALPLVEELSRTLEAATVPLGIHVRSAADLAD
                     ELLTELAYAYKTLLMEQSRRLFGLASSGRAQVPVVRAMQLLAERLTLSYRMYATPPRG
                     VWLEMHTLYQFALRRGFAQRGITDTGITPLDIYRRALLLAFAEPLKLMRGELDVVREW
                     IERYGDQAELLPANGSRNGQHGAFLVKTQRDQAGHSLSRRQQGPGQPNDSILCTLRLV
                     EVLREQADRMTQPARGQVGRPEARDVDTLDLLRRMARQWDSAPGRRHERLRTHSRVNV
                     HLGIDGIWQSLNAVDPQHVHASEWIITNEGAGGFALVHDQGEIEPIRVGDVIGIRMQR
                     DDNVHVCVVRWVLSDHPGHLELGVEEMAPTAHAVSIRQLHDEVGRNPEPVLLLPEVPA
                     LNQAPAILAPLVPLDTTCELNLGELQSRVRVKATQLLERTVSVQLLQFSPVN"
     gene            complement(8387..9601)
                     /locus_tag="VKZ48_05950"
     CDS             complement(8387..9601)
                     /locus_tag="VKZ48_05950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HLU77425.1"
                     /translation="MKRLLGRIGSFLYETGRRFIIDDSFKQAASLAYTTLLALVPLIT
                     IGLAVFSAFPAFQNFTVGINEFLSRAMLPSTVEQIVATHLDQFTRSAGRLTALGIVVL
                     GITAILLMQTIDASLQRIFSVRRKRPLMLRILTYWGVLTLGPLLIGASLSMTSYLLTR
                     PLIGGRYVDKLVLLDVVPFVLTALAFSSLYKIVPNRAVASRDALIGGLCAALLFEIMK
                     RGFAFYIAKFPTYTLIYGAFAAIPIFLLWIYLSWLVVLWGAVLAATLPEWRARQLRQP
                     DAEEVRMGERAQTLFAVLRALVDAQRTNAVISSADIAQRANVARQAAETMLEALARGG
                     WIVRASGGERWVLNCDPGAVTVAQVYDRTLYGAEESDDDLVRRVRRAHRQTLDLPLSA
                     LAAAEEPLNSPG"
     gene            9689..10279
                     /gene="wrbA"
                     /locus_tag="VKZ48_05955"
     CDS             9689..10279
                     /gene="wrbA"
                     /locus_tag="VKZ48_05955"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HLU77426.1"
                     /translation="MSDILVLYYSRHGAVRDMAQWIAHGVERVKGARARLRTVPAVST
                     VCEAVEPDIPAEGAPYCELDDLRECAGLALGSPTRFGNMAAPMKYFLDGTSGLWVQGA
                     LAGKPAAVFSSTASLHGGQETTLISMMLPLLHHGMLLVGLPYTESGLNTTTTGGTPYG
                     PTHVAGSDGRPIDETEKTLCIALGQRLAETALRLRR"
     gene            10276..10656
                     /locus_tag="VKZ48_05960"
     CDS             10276..10656
                     /locus_tag="VKZ48_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HLU77427.1"
                     /translation="MNAVTETPGWRFLYYGALTSLLLLIALCLAWEIRLAPIRPGGSW
                     LMLKALPLLWPLFGLLRVRRRTFQWSSFLILAYFTEGVMRGWADEGLSQQLALAEVAL
                     SVVFFFCVIYAARALGPRRARRAA"
     gene            complement(10887..>11668)
                     /locus_tag="VKZ48_05965"
     CDS             complement(10887..>11668)
                     /locus_tag="VKZ48_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010114575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HLU77428.1"
                     /translation="FGLDTQIDTTQIMPASRMVSSITGFVVQPNKAIVGANAFAHASG
                     IHQDGVIKARQTYEIMRAEDVGWTANKIVLGKLSGRNAFKQRLKELGIELESEESLNV
                     AFQKFKDLADKKAEIFDEDLHALVSDEAVSAENERWKLLTLNQTSGIGERPHATLVLN
                     DGGAEHRAESDGGGPVDAVFKAIESIARSGAELQLYSVNAVTQGTESQGEVTVRLAKA
                     GRIVNGVGADTDIVVASAKAYVNALNKLESKADRVNPQHAV"
BASE COUNT         2191 a         3800 c         3637 g         2040 t
ORIGIN      
        1 aaatcgaggg cgtcgtcagt ttcggctaca agggcccgaa acagcgccac acggtgagca
       61 tcgtaccggt cgcctaagcc cgtcccgctc acccaagccc tatccctgcg atagggcttt
      121 tttgtttcca ttgccggcgc aaagcgccct ccccttatga aattcatcga cgaagcgcgt
      181 atcgaagtgc acgccggcaa gggcggcgac ggcgccgtca gtttcaggcg ggagaagtac
      241 gtgccgcgcg gcggccccga cggcggcgac ggcggacgcg gcggcagcat ctacgcgatc
      301 gccgatcgca acatcaatac gctggtcgac taccgctata tgcgcatcca ccgggcgaag
      361 aacggggagc ccggccgcgg ctcgcaatgc tacggcaagg gcgccgacga catcatcctg
      421 cgcatgccgg tcggcacggt catcaccgac gccgagactg gcgaagtcat cgtcgatctg
      481 gccgagcacg gacagaaggc gctgctcgcc aaggggggcc agggcgggct cggcaacgag
      541 cgcttcaaat ccagcaccaa tcgtgcgccg cgacagtcca ccccgggcga accgggcgaa
      601 agccgcgcgc ttcatctgga actgaaggta ctcgccgacg tcggcctgct gggcatgccg
      661 aacgcgggca aatccacctt catccgtgcc gtctcgagcg ccaagcccaa agtagcggac
      721 taccctttta ccaccctgca gccgaacctc ggtgtggtgc gcgtcgacac cgaccggagc
      781 ttcgtgatcg ccgatatccc cggcctgatc gaaggcgcag ctgaaggtgc cgggctcggc
      841 catcagttcc tgcgccatct cgcccgcacg cggctgctgc tgcatctggt ggatctggcg
      901 ccgctggatc cggacgccga tccggtaaag aatgcgcgcg ccatcgttgc cgaattgcgc
      961 aaatatgacg aagcgctcta tcgcaaaccg cgctggctgg tgctgaacaa ggcggatatg
     1021 ctcccccctg aacagcgcga cgaggtggtg gccgaattcc tgcgccgcta ccgctggaag
     1081 ggaccgcact tcctcatctc ggcgctcacc ggagaaggat gccgcgagct gtgtttcgcg
     1141 atcatggaac atctggagaa acaacaatga aaggcgcgat cggcgcagca cgtcgattag
     1201 tcgtcaaggt aggcagcgcg ctggtcacgc atgatggcga aggggtggat tacgaggcca
     1261 tcgcgcactg ggcggccgaa atcgccgagt tgcggcgcgg cggtagggag gtggtgctgg
     1321 tttccagcgg cgcggtcgcc gaaggcctga agcgcctggg ctggcgcaag cggccgcgca
     1381 gtatgcacga actgcaggct gcggcggccg tcggccagat gggcctggta caagcctacg
     1441 agagctcttt ccgtgtgcac ggcctgcaga cagcacagat tctgctgacg catgccgacc
     1501 tcgcggaccg tcagcgctat ctgaatgcac gctccaccat ccgcacgctg ctgaagctcg
     1561 gcgtagtgcc ggtcatcaac gagaacgaca ccgtcgtcac cgacgaaatc aagttcggcg
     1621 acaacgatac gctcgcggca ctggtcacca atctgatcga agccgacctc ctcgtcatcc
     1681 tcaccgacca ggccggcctg tacaccgccg acccgcgcaa ggcgccgacg gcgcaactcg
     1741 tgcgcaaagc gcaggcgggc gacccggcgc tcgaacgcat ggccgggggc gcagggactc
     1801 aaatcagtcg cggcgggatg atcaccaagg tgcgtgccgc agcccgtgcg gcgcgcagcg
     1861 gcgcacacac cgtcatcgcc tcgggacgcg agccgaaatt gctatcgcgt ctgatcgccg
     1921 gcgaggcgat cggcagcctg ctctacgcgg agcacgagcc gctggcggcc cgcaagcagt
     1981 ggatggcgga tcacgtccaa ctgagcgggc gcctgcaact cgatgcgggc gctgcaagag
     2041 cgctgcgtac ggaaggcaag agtctgctgc cggtgggtgt acgcgcggtc gaaggcgatt
     2101 ttgagcgcgg cgaagtcgtc gcctgcatcg atccggaagg ccgcgaagtc gcacgcggcc
     2161 tgatcaatta cggcgcggcg gaaacacgcc gcatcctcgg ccactcaagc caggatatcg
     2221 aacgcatcct cggctatgtc gaggaaaccg agctgatcca tcgcgacaat ctggtgctga
     2281 tctgagtgtc gcggttcact cgtacgggct gaactgcttg accagcggat gcaccccgtt
     2341 acgcagcgca cccacggcct catcggcgct ctgcaccggc cccctcagcg ggcagaggaa
     2401 attgcgatag agggttcgtc gcggcacgtc gtatcggaac gcttcccact gcggatcgcg
     2461 cgcgagtgac catgtgccgt cgcgccggcc atacgcatac gtcttgcgct cgtccgactt
     2521 gcagcgcacc ccctcgtaca tcacgttgcg cgtgtcctcg tcgcccgtca ccaccagcgt
     2581 atagcgcacg acctgatcct ggcccaccga cacagaggct gcatccacgt agaacttcat
     2641 ggtcgtgggt cggccgggat cgaatgcgat gagattcttc tgctccggtg cggccggcag
     2701 attgagttgc tcttccttga aaatctccac ctcttcgggc accgcgccgc gcgggtcgcg
     2761 gtcgaacagg ggaatctgag catgcgccca cagcgtggca agcagggtgc acaacagcag
     2821 aagtctcaac atggcttgcg atcagaaggg gcgcgccggc aggcggcggc ttcaaatacg
     2881 ttcgggctgg cgcgccggat ggcggcgcgg attgcggtgg agaaaacggg aaagctcgga
     2941 cagcgcctgc tgatacacct cacgcttgaa ctcgatgaca ttgtccagcg gcgcccagta
     3001 gtcgctccag cgccaagcgt cgaactccgg atgctcgcag gcccgcaacc gtacatcgca
     3061 gtcgcggccg accagtcgca acagatacca gatctgcttc tggccgcgat agctcccgcg
     3121 ccattcgcgt ctgatccact gcgccggcac gtcgtaacgc agccatccgc gcgtccgacc
     3181 gaggattttg atgtgatgag gctcgagccc ggtttcctct tgcaattccc ggtacatcgc
     3241 ctgttcaggc gtttcgccgt acttgatgcc gccttgcggg aactgccaag agtgctcctt
     3301 gatccgtttg ccccaaaaaa cctcgttctt cgcgttgcac agaatgatgc cgacgttcgg
     3361 ccgatagcct tctcggtcga gcatatccag ccttgaaagc aataagtctg cgacgattct
     3421 tccacaattc ccaaggcatg gaaagcaagc ccggggatcg cggcccctgc gctacaattc
     3481 acggcttttc cggagctttg tcgatgcgcg tctcgcagtt ctttctgggc accctcaagg
     3541 aagcgccggc cgatgcggaa atcgtcagcc ataagctgat gctccgggcc ggccttatca
     3601 gacgcctggc aggcgggatc tatacctgga tgccgctcgg cctgcgtgtc gtgcgcaagg
     3661 tcgaagccat tgtgcgcgag gaaatgaacc gcgccggcgc gatcgaattg ttcatgcccg
     3721 ccgtgcagcc tgcggagtta tggcaggaat ccgggcgatg gcagaaatac ggccccgagt
     3781 tgctgcgcgt gaaggaccgg cacgaccgcg atttctgcat cgggcccacg cacgaggaag
     3841 tgatcaccga tctcgtgcgg cgcgaagtaa agagctatcg ccaactgccg ctcaacttct
     3901 atcagatcca gaccaagttc cgcgacgaaa tccgtccgcg tttcggcgtc atgcgcggtc
     3961 gcgaattcac gatgaaggac gcctattcct tccacgcgga ctacgaggac ctgcagcgcg
     4021 agtacgccaa catgtacgag acgtacacgc gcattttcac ccggctgggt ctgggattcc
     4081 gcgccgtggc ggccgatacc ggatccatcg gcggcaccgg ctcgcacgaa ttccacgtcc
     4141 tcgccgactc gggcgaagac gccatcgcct tctgcccgca atccgattac gcggcgaacg
     4201 tggaactggc cgaagcgctg gcgccggata cgccgcgcgc cgcagccggc gaagcgctcg
     4261 ccaaagtccc gacgccgggc aaaacccgct gcgaagacgt cgccgccctc ctcgatctgc
     4321 cgttggaacg cacggtgaag gccatcgccg tcatgcatga caatgaattc gtgctgctgc
     4381 tcctgcgcgg cgatcacgaa ctgaacgaaa tcaaggcgca gaaagtggaa gggctcgatc
     4441 cgttccgctt cgccacggat gcggagatcc aagagcatct gggctgcaag cccggctaca
     4501 tcggccccgt ggatatcgac gccaaaattc gagtgatcgc cgatcgcagc gtcgcggtga
     4561 tgagcgactt cgtctgcggc gcaaacgagg aaggctttca cttgcgcggc gtcaactttg
     4621 gccgcgacct gcccgaaccg gcatgggtgg ccgacattcg caaggtcctc gccggcgatc
     4681 cgagtccgga cggcgaaggc acgctcgaga tcgtacgcgg catcgaggtg ggccacatct
     4741 tccaactgcg caccaactac agtgcgaaga tgcaggccgt cttcctcgac caggacggca
     4801 agcagcaacc attcgaaatg ggctgttacg gcatcggcgt aacacgcatc gtcggtgccg
     4861 cgatcgagca gaatcacgac gagcgcggca tcatttttcc cgatcccatc gcgccctttc
     4921 agctcgccat cgtgccggtg ggctatcaca agagcgaagc ggtacggcag gccgcggacc
     4981 ggctttacgc gggtctgcgc gcggcaggaa tcgacgttct gctcgacgat cgtgaggaac
     5041 gtcccggcgt gatgttcgcg gacatggaac taattggcat tccgcatcgg gtagtggttg
     5101 gagaacgcgg tctcaaggat ggcaaaatcg agtaccaggc gcgtcgcgac aaagcggcgc
     5161 agaccgtaga actcgaacag gccttgccct tcctgcaaga ccgtctatgc ccaagcacta
     5221 cagacgactc ggagccgcgc tcgcgctgat ctgcgctttt tatgcgccgg ctgcgccggc
     5281 cggcgatcag atgtacgaac cgctatcggc cagcgtgcgc tcggcgatgg cgcggtccgt
     5341 cgctgatgaa ggtaacgtgc gcctcgcctt cgccgatccg caagatggtt accggtggtt
     5401 ggttgcgatg tcgaagcggc tggaaaagcg cattcccgat gccggatttc gcgaagagtt
     5461 gctgaagatg gtgcattacg agtcgacgcg cgccggcctc gatccgcaac tcgtgctcgg
     5521 catcatccag gtcgaaagca actttcgcaa atacgccgtt tccaaagccg gtgcacgcgg
     5581 actgatgcaa gtcatgccct tctgggtgga attgctgggc gacggccggc acaacctgtt
     5641 ccatgtgcgc acgaatctgc gctacggctg caccatcctg cgtcactacc tcgacatcga
     5701 aaagggcaac ctcttccgcg ccctgggccg ttacaacggc agtctcggac gcgaagccta
     5761 tccgaaccgc gtcatggcgg cgtggcgcac cttcaaatac gacggcaagc tgatctgagc
     5821 cgtcgcactc aaccggcaaa ggtcttcgcg atcgtcgcct gaatctgctc cgccgccgca
     5881 cgcggatccg ccgcctggcg gatcggccgt cccacgacaa tgtaatccgc gccgttggtg
     5941 aaggcctgcg ccacatcgac cgtgcgcttc tggtcgtcgg cggggcggtt ctccacaggc
     6001 cggatgcctg gcgtgaccac catcacccgc tcgcccaact cgcttttgag catcggcgct
     6061 tcgaggccgc tggagatgac gccgtcgcag cctgccgcaa gcgctcgccg cgcgcgtgaa
     6121 agcaccagct tttccacatc gcaatcgaaa cccaggtcgt ccagatcgcc gcggtcgagg
     6181 ctggtcagca ccgtgacggc gagtatcttc acttcgccct tgtccgcgcc cgccgcttcc
     6241 atgatggcct gattgccgtg cacggttgcg aaggtcaccc cgcgattacg caagccgcgc
     6301 actgcgctgc gcaccgtttc cggcacatcg aaaaatttca ggtcggcgaa gactttcttg
     6361 tccgccttca ccaggcgttc cagcagctcg aaataacggc ccgacatgaa cagttccagg
     6421 ccgagcttgt agaagtgcac gctgtcgccc agggtttcca ccagcttctg cgcctcgtcg
     6481 atattcggca aatcgagcgc gacgatgagt cgctcgcgcg gcgggatgtt cttggcggaa
     6541 agcaatgctt gtgtcatcgg agtgtcctgc atgagcgccg ctgcggcgac ggccgcccgg
     6601 caacggttga gagcgcgcat tttagcctct gccgcggtga aggattgcgc gcatcgtcac
     6661 ctgccgcccg ctgtcgcgtt tgcgtcatcg ccgccattcg cgagacgtcg tctttccgcc
     6721 gcgcccgccc gacacccgcc acccccgtga tagttgtcac gacttgcacg gcgcgttgtc
     6781 ggttttcgcg agcaaaactc gtacgcaagc gcgcgaaacg cgtggcatgg agcttgcgaa
     6841 ataccggtgc aaagctgctt cacggcacac caacgacgac acaacaggag ccgtatgtct
     6901 ttgcacctca gtctgccgcc gtccggcgag cgcgttgacg cgcaaccgga acccgatccg
     6961 ctgcaaatcg ccgcatgggt cgacggcctg cgcaatgaag aacccctgcg cgcggcgcaa
     7021 caactcgtcg atttcctcgt gccgatgaac cgcacgcgta tgcgcgtcgc cctgcgccag
     7081 gaagtggcgg aactcatcgt gccggcggcg ctgccgctgg tggaggaact gagccgcaca
     7141 ctggaggcag ccacggtgcc gctggggata cacgtccgct cggccgcgga tctggccgac
     7201 gaactgctca ccgagttggc ctacgcttac aagacgctgc tgatggagca gtcgcgccgc
     7261 ctgttcggtc ttgcctccag cggacgcgcc caagtgccgg tggtacgcgc aatgcaattg
     7321 ctcgccgagc gcctcaccct cagctatcgc atgtatgcca cgccacctcg aggcgtatgg
     7381 ctcgaaatgc acacgctcta ccagttcgcc ctgcgccgtg gattcgcaca gcgcggcatc
     7441 accgacaccg gcatcacacc cctggacatc tatcgccgcg ccctgctgct ggctttcgcc
     7501 gagccgctga aactgatgcg cggcgaactg gacgtggtgc gggagtggat cgaacgctac
     7561 ggcgaccagg cggaactgct gccggcgaac ggctcacgca acggccagca tggcgctttt
     7621 ctggtcaaga cgcagcgcga tcaggccggc cactcgctgt ccaggcgcca acaaggaccc
     7681 ggacaaccga acgattcgat cctctgcacc ctccgtctgg tcgaagtgct gcgcgaacag
     7741 gcggaccgca tgacgcagcc cgctcgcggg caggtcggcc ggccggaagc gcgagacgtc
     7801 gataccctgg atttgctgcg gcggatggcg cgccaatggg acagcgcacc cggacggcgg
     7861 catgagcggc tgcgcaccca ctcaagggtg aatgtgcatc tgggcatcga cggaatctgg
     7921 caatccctca acgcggtcga cccgcagcat gtccacgctt ccgaatggat catcaccaac
     7981 gaaggcgccg gcggctttgc gctggtgcac gatcaagggg agatcgagcc catacgcgtc
     8041 ggcgacgtaa tcggcatccg catgcagcgc gacgataacg tccatgtttg cgtcgttcgc
     8101 tgggttctgt ccgatcaccc gggccatttg gagcttggcg tggaagagat ggcgcccact
     8161 gcgcatgcgg tatcgatccg ccagttgcat gacgaagtcg gtcgcaaccc cgagccggtc
     8221 ctgctgttgc cggaagtccc cgccctgaac caggcgcccg ccatcctcgc accactcgta
     8281 ccgctggaca cgacctgcga gttgaatctt ggcgagttgc aatcgcgcgt gcgcgtcaag
     8341 gcgacgcaat tgctggagcg caccgtcagc gtgcaactcc tgcagttcag cccggtgaat
     8401 tgagcggctc ctccgcggcg gcgagcgcgc tgagcggcag atcgagtgtc tgccgatggg
     8461 cccgccgcac ccgccggacg agatcgtcat ccgactcctc ggcgccgtac aaggtccggt
     8521 cgtacacctg tgcaacagtg acggcgccgg gatcgcaatt cagtacccag cgctcgccgc
     8581 cggaggcgcg cacgatccag ccgccgcgcg cgagcgcctc gagcatcgtc tccgcggcct
     8641 ggcgcgcgac gttcgcgcgc tgagcgatat cggccgagct gatgacggcg ttggttcgct
     8701 gcgcatcgac cagcgcgcgc aggacggcaa acaaggtctg cgcccgttcg cccatgcgta
     8761 cctcttccgc gtcgggttgc cgtaactgcc tcgcccgcca ctccggcagg gtcgcggcaa
     8821 gtaccgcgcc ccacagcacc accagccacg atagataaat ccacagcaga aaaatcggaa
     8881 tggcggcgaa tgcgccgtag atcagcgtgt aagtagggaa cttggcgatg tagaacgcga
     8941 agccgcgttt catgatttcg aacagcagcg ccgcgcacaa cccgccgatc aacgcgtctc
     9001 gcgatgcgac ggcacgattg ggcacgatct tgtacaggga actgaaagcc agcgccgtca
     9061 gtacgaacgg caccacatcg agcagcacga gcttatcgac ataacggccg ccgatcagcg
     9121 gccgagtgag caaatacgaa gtcatcgaca gactggcgcc gatcagtagc ggcccgagcg
     9181 tcagcacccc ccaataggtc aggatgcgca gcatcagcgg ccgcttgcgc cgcacggaga
     9241 aaatgcgctg cagcgatgca tcgatggtct gcatcagcag aatcgcggta atgccgagca
     9301 cgacgattcc gagggcggtg agccgtccgg cgctgcgggt gaactggtcg agatgggtgg
     9361 cgacaatctg ctccaccgtc gaggggagca tggcgcggga caggaattcg ttgatgccga
     9421 cggtgaaatt ctggaaagcc gggaacgccg agaataccgc cagaccgatg gtgatcaacg
     9481 gcaccagcgc cagcaatgtg gtgtaagcga ggctggccgc ctgcttgaag gaatcgtcga
     9541 tgatgaaacg acgccccgtt tcgtagagga aggaaccgat acggccgagg agtcgtttca
     9601 tgttcacgga tcaatagggc gtctatgtgc catgccgcgg aggatcgact cgtataatag
     9661 caaccgtctt tcccgcgctt ccctgctgat gtccgacatc ctcgttctct attacagccg
     9721 ccatggcgcg gtccgcgaca tggcgcaatg gatcgcccac ggcgtcgagc gcgtcaaggg
     9781 tgcgcgcgca cgcctccgta ccgtcccggc cgtatcgacc gtgtgcgagg cagtcgagcc
     9841 tgacatcccc gccgaggggg cgccctattg cgaactggat gatctgcgcg aatgcgcagg
     9901 actcgcgcta ggcagcccca cgcgtttcgg caacatggcg gcgccgatga aatattttct
     9961 cgacggcacc agtgggctgt gggtgcaagg cgcgctggcg gggaaacccg ccgcggtgtt
    10021 ctcttccacc gcgagcctgc acggcggcca ggaaacgacg ctgatttcga tgatgctgcc
    10081 cttgctgcat cacggcatgc tgctggtcgg cctgccgtac acggaaagcg gactcaacac
    10141 cacgaccacc ggtggcacgc cgtacgggcc gacgcatgtg gccggcagcg acgggcgacc
    10201 gatcgacgag acggaaaaaa cactctgtat cgcgctggga cagcgcctgg ccgaaaccgc
    10261 cctgcggctg cggcgatgaa cgcggtgacg gaaacgcccg gctggcgctt cctgtactac
    10321 ggtgcgctga ccagcctgct gctgctgatc gcgctttgtc ttgcgtggga gattcggctc
    10381 gcccccatcc ggccgggcgg ctcgtggctg atgctgaagg cactgccctt gctgtggccg
    10441 ctgttcggcc tgctgcgcgt ccgtcgccgc accttccagt ggtcctcctt cctgatcctc
    10501 gcctatttca ccgaaggcgt gatgcgcgga tgggcggacg agggactctc gcagcaactt
    10561 gcgctggcgg aagtcgcgct gtcggtggtg ttctttttct gcgtgatcta tgcggcccgc
    10621 gcgctgggac cgcgacgcgc caggcgcgcg gcttgaactg cgaaagaccg aaacacagag
    10681 atcatagaga agcacggagt gcactgaaaa aagcactcta atccagccat cccgcgaaag
    10741 cgggcacccg gtagtgcatt aaagaccgga tgcacgccac tgtacgtgcc gtgcctgcgg
    10801 tgcaggcttt tgtggccgta tggagaggcg gaaccacagt ggttatgcca ccgctctccg
    10861 tggtctccgc gtttcaagct attgaatcag accgcgtgct gcgggttgac ccggtcggcc
    10921 ttactttcga gcttgttcaa cgcgttcaca taagccttgg ccgacgccac gacgatgtcg
    10981 gtatcggcgc cgacaccgtt cacgatgcgc cctgccttgg ccagtcgcac cgttacctcg
    11041 ccctgcgact cggtgccctg cgtgaccgca ttgacggagt agagctgcag ttccgcgccg
    11101 ctgcgcgcaa tgctttcgat cgccttaaat accgcgtcga ccggcccgcc gccatcggac
    11161 tccgcccgat gctcggcgcc gccgtcgttc agcaccagcg tggcatgcgg acgctcgccg
    11221 atcccgctgg tctgattcaa ggtcagcagt ttccagcgtt cgttctcggc gctgactgcc
    11281 tcgtccgata ccagcgcgtg cagatcctcg tcgaagatct ccgccttctt gtcggcgagg
    11341 tccttgaact tctggaaggc gacgttgagc gactcttcgc tttccagttc gatgcccagt
    11401 tccttgaggc gctgtttgaa ggcgttgcga cctgacagct tgcccagcac gattttgttc
    11461 gcggtccagc ccacgtcctc ggcgcgcatg atctcgtagg tctggcgcgc ctttattacg
    11521 ccgtcctgat ggatgccgga ggcgtgcgca aaggcgttgg cgcccacgat ggccttgttc
    11581 ggctgcacca cgaaaccggt aatggacgaa accatgcgcg aggcaggcat gatctgcgtg
    11641 gtgtcgattt gcgtgtcgag gccgaaga
//
LOCUS       DATGOU010000098         3408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3846091, whole genome shotgun sequence.
ACCESSION   DATGOU010000098 DATGOU010000000
VERSION     DATGOU010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3846091"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1887)
                     /locus_tag="VKZ48_05970"
     CDS             complement(136..1887)
                     /locus_tag="VKZ48_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HLU77429.1"
                     /translation="MSMITVSLVQGTSAWHQHRAKHFNASDAPAMMGCSPYKTRAQLI
                     REVAAGITPEVDAATQRRFDDGHRFEALARPLAEKIVGEELYPCTGVLSGTKLSASFD
                     GLTMLGDVAWEHKTLNDELRKIVNGETAGSELPEHYRVQMEQQCAVSGAERVLFMATK
                     WDADGNLVEERHCWYTPDLDLRARIIAGWEQFEKDVAAYVPEPVVAAPVATPVAGFGA
                     LSLRVEGRVLSSNMDKFRADAEAFIARLPKPGDLQTDQDFADAESAVKACAEAESRMK
                     AAKDAVLAQMSDVDAVLRAADEVSETIRAARLALDKVVKAEKESRKAALIRAGVDAVQ
                     QHYASINATLGEYAVGVPASLTADIAASIKGLKTLSSMRDKIDAAVAQAKINASQEAD
                     RRRLCAEVLREHAGAAMHLFVDHVQLVVSKSPDDLRNLIAARIAEHEQREAQRLEAER
                     ERIRAEEQAKAEQAAREDAGRAQVAEAKSHGAMGNAQQAVAAPIAAAPAYVGTAPTAA
                     TGAASRSASPIRLGQINAAIAPLSITADGLAQLGFSPVGHERAAKLYAASDFDRIRAA
                     LIQRLQSAALPVMEAAA"
     gene            complement(1884..2651)
                     /gene="bet"
                     /locus_tag="VKZ48_05975"
     CDS             complement(1884..2651)
                     /gene="bet"
                     /locus_tag="VKZ48_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HLU77430.1"
                     /translation="MSKLVVSQAAALAKSLSLAPSDTAELVAALKATAFKGQVSDAQM
                     TALLVVANQYGLNPWTKEIYAFPDRQNGIVPVVGVDGWSRIINSHPQFDGMDFDQDDE
                     RCTCIIYRKDRTHPIRVTEYMAECKRDGVGPWKSHPRRMLRHKAMIQCARIAFGFVGI
                     YEQDEAEHILEAQVSEIPADRPANPAPYPAESFEKNLPRWRELIESGSKTADEIIALV
                     SSKGRLTAEQIKAIRDCEEVRDAEVVEPEQEAREAEA"
     gene            complement(2823..2987)
                     /locus_tag="VKZ48_05980"
     CDS             complement(2823..2987)
                     /locus_tag="VKZ48_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77431.1"
                     /translation="MSDPKVIPFPRKPKPRPTNAERVSQMDAEWLRSFVPQIVKRDAA
                     NDKTPGPEAA"
     gene            complement(2980..3201)
                     /locus_tag="VKZ48_05985"
     CDS             complement(2980..3201)
                     /locus_tag="VKZ48_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77432.1"
                     /translation="MNRTEIVCILVMCGAMLAGLGMFLYEVHAGRESRELTRQHRDAC
                     AEQGLPATECCRVGLSWVCPLAMKESNDE"
BASE COUNT          544 a         1117 c         1095 g          652 t
ORIGIN      
        1 cattcgccag cacggtcaag atcagctgcc gacggatcac ggctgattcg cgcttgcgct
       61 gctcgcgtgc attgcgcggc ggatcgctct cgggcgctgg cacgctggca atcgggaagg
      121 tgagggcgcg caggttcatg ctgccgcctc cattaccggc agcgcggccg actgcaatct
      181 ctggatcagc gcggcgcgga tgcgatcgaa gtcgctggcc gcatacagct tggcagcacg
      241 ctcgtggccg accggtgaga atccaagctg cgccaggcca tcggcagtga tcgacagcgg
      301 ggcgatggcg gcgttgatct gaccgagcct gataggggat gcgctacggc ttgcagcgcc
      361 agtcgccgca gtgggagcag tgccaacgta ggcaggtgcg gcagcgatcg gagcagccac
      421 agcctgctgg gcgtttccca tcgccccgtg cgacttcgcc tccgccactt gtgcgcgccc
      481 cgcgtcctcc cgcgccgcct gttctgcctt ggcctgttcc tcggccctga tgcgctcgcg
      541 ctccgcctcc aagcgctgcg cctcgcgctg ctcatgctcg gcaatgcgcg ccgcgatcag
      601 gttgcgcagg tcgtccggtg acttcgacac aacgagctgg acgtgatcga cgaacaggtg
      661 catggcggcg cctgcatgct cgcgcagcac ttcggcacac agccggcggc ggtccgcctc
      721 ctggctggcg ttgatcttcg cctgcgcgac ggcggcgtcg atcttgtcgc gcatcgacga
      781 cagggttttc aggcctttga tgctggcggc aatgtctgcg gtcagtgacg caggtacgcc
      841 gacggcatac tcgccaagcg tggcattgat gctggcgtag tgctgctgca cggcgtcaac
      901 gccagcgcga ataagcgcag ccttgcggct ttccttctca gccttgacga ccttgtcgag
      961 cgccaatcgg gcggcgcgga tcgtctcgga cacttcgtcg gcagcacgca acacggcgtc
     1021 cacgtcggac atttgcgcga gcacggcgtc cttcgcggct ttcatgcggc tctcggcctc
     1081 ggcgcaggcc ttgacagcgc tctcggcgtc ggcgaagtcc tggtcggtct ggagatcacc
     1141 cggcttcggt aggcgggcga tgaacgcctc ggcgtcggcg cggaacttgt ccatgttgga
     1201 cgagagcacg cggccctcga cgcgcaggga tagagcgccg aaaccggcaa ctggcgtggc
     1261 gacaggcgcc gccacaacag gctcgggcac gtatgcggca acgtctttct cgaattgctc
     1321 ccagccggcg atgattcgag cgcgcagatc gaggtccggc gtgtaccagc agtgccgttc
     1381 ctcgaccaga tttccgtcgg catcccattt cgttgccatg aacaggacgc gctcagcgcc
     1441 gctaaccgcg cactgctgct ccatctggac gcgatagtgc tcgggcaatt ccgagccggc
     1501 cgtctcgccg ttgacgatct tgcgcagttc gtcattgagc gtcttgtgct cccacgctac
     1561 gtcgccaagc atcgtcagcc cgtcgaacga tgccgacagc ttcgtgccgc tcagcacgcc
     1621 ggtgcatggg tacagttcct cgccgacgat cttctcggcc agcggacgcg ccagggcttc
     1681 gaaccggtgc ccgtcgtcaa accgacgctg cgtggccgca tccacctccg gcgtgatgcc
     1741 ggcggcgact tcacggatca gctgggctcg ggtcttgtac gggctgcagc ccatcatggc
     1801 cggcgcgtca ctggcgttga aatgcttggc gcggtgctgg tgccaggcgc ttgttccctg
     1861 gacgagcgaa acggtaatca tgctcatgct tcggcctccc tggcttcctg ttcgggttcc
     1921 accacttcgg cgtccctcac ctcctcgcag tcgcggatgg ctttgatctg ctcggcggtt
     1981 agtcgaccct tgctcgacac cagcgcgatg atttcatcgg ccgtcttgct gccggactcg
     2041 atcagctcgc gccacctcgg caggtttttc tcgaaggatt cggccgggta gggcgccgga
     2101 ttcgcggggc gatcagccgg gatctccgaa acctgcgcct caagaatgtg ctcggcctcg
     2161 tcctgttcgt agatgcccac gaagccgaac gcgatgcgcg cacattggat catcgccttg
     2221 tgccgcaaca tgcgtcgcgg gtgcgacttc cacggcccga cgccgtcacg cttgcactca
     2281 gccatgtact cggtgacgcg gatcgggtgc gtgcggtcct tgcgatagat gatgcaggtg
     2341 cagcgctcgt cgtcctggtc gaagtccatg ccatcgaact gcgggtgcga attgatgatt
     2401 cgcgaccatc catcgacacc gacgaccggg acgatgccgt tctgacggtc agggaatgcg
     2461 tagatttcct tcgtccacgg gttcaggccg tactgattgg cgacgaccag gagcgcggtc
     2521 atctgcgcat cgctgacctg gcccttgaac gcggtggcct tgagggcggc gaccagctcg
     2581 gcggtgtcgc tgggcgcgag cgacagggac tttgcgaggg cggcggcctg cgaaacaaca
     2641 agcttgctca tatcaatctc cgcgcgctat cgggcgcgat ccgtgtggaa attcccgcgc
     2701 ggatacggcc gcgctccgtc tcgaattcat gccgtctgcc acaaccggga cggcgctcgg
     2761 tgggaggatc cggcagcgca tggggtcgca tgccggcgcg ctgtggctgc gcgggggagg
     2821 gttcatgccg cttcgggccc cggcgtcttg tcattcgctg catcgcgctt cacgatctgc
     2881 ggcacgaaac tgcgcagcca ttcggcatcc atctgcgaca cgcgctcggc gttcgtcggg
     2941 cgcggcttgg gcttacgcgg gaaggggatc actttcgggt cactcatcgt tcgactcctt
     3001 catggcgagc gggcaaaccc acgacaggcc gacccggcag cactcggtcg caggcaggcc
     3061 ctgttctgcg catgcgtcgc ggtgctgtcg ggtcaattcg cgggactctc gaccggcgtg
     3121 cacctcgtag aggaacatgc cgaggccggc gagcattgct ccacacatga cgaggatgca
     3181 tacgatttcg gtgcggttca tgcaaccctc ctagaaaaca gcgagcgcag ccaggtgagc
     3241 gggcacatcg cgacaaataa atacgtgcga gcatagacaa cgctcacctc gttgtacttg
     3301 cgccatacct cgtttgcacg agctcgggcc ttgaccagcg cgtcaacgcg ctcgcccaac
     3361 agaatacagt cgtcaagcgc gatgacctaa acttcataat cgcacacc
//
LOCUS       DATGOU010000099         5124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_291313, whole genome shotgun sequence.
ACCESSION   DATGOU010000099 DATGOU010000000
VERSION     DATGOU010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291313"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VKZ48_05990"
     CDS             <1..201
                     /locus_tag="VKZ48_05990"
                     /inference="COORDINATES: protein motif:HMM:NF017727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HLU77433.1"
                     /translation="PLTWFCFLAVIGVALIRIAGDIALLPWSLRAGLLVTAAVAWLAV
                     LIPWALRYAPIYLRPRADGKPG"
     gene            222..818
                     /locus_tag="VKZ48_05995"
     CDS             222..818
                     /locus_tag="VKZ48_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HLU77434.1"
                     /translation="MPNSLSLAAPGFNDPIGLLKACHMRIEQRCALLGRIMEHMAKHG
                     ADQQAKQACTQVLHYFNTSGPLHHLDEEENLFPALLRHAKRSQKKKLQALIADLEADH
                     DEMERAWEAMRANLEGVVNDTGPALNPDIVDRFQTIYFAHIQREEAEAFSVAVDCLPP
                     EELERIGRAMAARRNVAYPDADVVVPKSEALSQSARAV"
     gene            864..1088
                     /locus_tag="VKZ48_06000"
     CDS             864..1088
                     /locus_tag="VKZ48_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77435.1"
                     /translation="MSMRTPNRRSTMRTRRLAYEPRAHIPAAITFAALTFSTLRGVDF
                     LVLAILLGYVALSTYLPRPAAFRKAASRYL"
     gene            1289..2290
                     /locus_tag="VKZ48_06005"
     CDS             1289..2290
                     /locus_tag="VKZ48_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HLU77436.1"
                     /translation="MKPELVCPAGALPALKAAVDAGADCVYIGLPDNTNARNFPGLNF
                     DMTSAREGIRYAHAAGRQVFLAINTYAQAAAMDRWYRAVDTAAELGVDAVILADPGVM
                     RYAAEKHPDLRLHLSVQGSATSAAAINLYARSFGVQRAVLPRVLSLQQVEQVVRESNV
                     EIEVFGFGSLCVMAEGRCSLSSYATGESPNTHGVCSPASAVRWEPKDDGMDARLNGIL
                     IDRFDNDERAGYPTLCKGRFEVANETYYAIEEPVSLNTMSLLPALAQMGVAAIKIEGR
                     QRSPAYVSQVTRAWRAAIDAADRNPARFAPDPAWRNALARVSEGQQVTFGAYERKWR"
     gene            2350..3243
                     /locus_tag="VKZ48_06010"
     CDS             2350..3243
                     /locus_tag="VKZ48_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019255311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HLU77437.1"
                     /translation="MRLSLGPLLYYWPRDIVQAFYARMAETAVDTIYLGELVCSRRHE
                     LRVDDWLALARELKAAGKQVVLSTQALIESPADMRTLRRLCENDEFLVEANDASALGS
                     MQEGAAFIAGPHLNAYNGQTLQWYAELGAVRWVMPVEVSQAALRAIQRERPAGMETEV
                     FAWGRLPLAFSARCFTARHHNLPRDDCRYRCIEDPDGLPMRTQEGQPFLTLNGTQTQS
                     AGYYDLSAELDGLRDAGVDVLRISPQSAGTAEVIDTFARAIAGTLPPDERDERLRAVA
                     AHRCNGHWHGRPGVEYVARPR"
     gene            3224..3673
                     /locus_tag="VKZ48_06015"
     CDS             3224..3673
                     /locus_tag="VKZ48_06015"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HLU77438.1"
                     /translation="MLHAPAEPLRRLIARLPARPPAVALATALNLALRPKLNEAHRAM
                     LHGRVVLIGVTDAGIECRISWSGTRFVAHARHTPHDVAIRASVRDFYALAFHTEDPDT
                     LFFQRRLRIEGDTALGLGIKNLLDSMELPGWIAGLHARWMQRQSRYI"
     gene            3752..4009
                     /locus_tag="VKZ48_06020"
     CDS             3752..4009
                     /locus_tag="VKZ48_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004528365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HLU77439.1"
                     /translation="MTEPHLHILQDQIYPFDARGIAKRFRHAAIFGAFDALRPGEIMR
                     FVNDHDPLPLLQQFQQRYGEHVSIEYKQREPGAIVIDFQHV"
     gene            4064..5122
                     /locus_tag="VKZ48_06025"
     CDS             4064..5122
                     /locus_tag="VKZ48_06025"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HLU77440.1"
                     /translation="MEMLTTDLPRLRIKGRSLLPIVQGGMGVGVSAHRLAGSVARQGA
                     VGTLSSVDLRRHHDDLMARTHKSGDKALIDAANLEALDREVTAALALADGRGLVAVNV
                     MRAVSEYAHYVQQSCKSGAQAIVVGAGLPLDLPDLTADYPDVALIPILSDARGIAVLV
                     RKWMRKNRLPDAIVIEHPRYAGGHLGAAKIEDLNDPRFDFENVIPETQKLFEQLGIEP
                     IPLICAGGISTPERVRELIGLGASAVQLGTPFAVTEEGDAHINFKKVLAEAKPEDIVE
                     FMSNAGLPARAVRTPWLDTYLKKLSIHEARAKIRQCTFKFDCLEACGFRDGISKIGQF
                     CIDQRLSLAVKGDVRNGL"
BASE COUNT          899 a         1723 c         1602 g          900 t
ORIGIN      
        1 ccgctcacct ggttctgctt cctggcggtg atcggcgtgg cgctgatccg catcgccggc
       61 gacatcgcgc tgctgccctg gagcctgcgc gccgggctgc tcgtcaccgc ggccgtcgcc
      121 tggctggccg tattgattcc ctgggcgttg cgttacgccc ccatctacct gcgtccgcgc
      181 gcggacggta aaccaggtta aacgctttta gaggtatcga aatgcccaac tccctgtctc
      241 ttgccgcacc gggcttcaac gatcccatcg gactactgaa ggcctgtcat atgcgcatcg
      301 aacagcgctg tgcgctgctc ggccgcatca tggagcacat ggccaagcac ggcgccgacc
      361 agcaggccaa acaggcctgc acgcaggtgc tgcactactt caacacctcc ggcccgctgc
      421 atcatctgga cgaagaggaa aatctcttcc ccgcgctgct gcgccacgcc aagcgctcgc
      481 agaagaagaa actgcaggcg ctgatcgccg acctcgaagc cgaccacgat gagatggaac
      541 gcgcatggga ggcgatgcgc gccaacctgg aaggcgtggt caacgacacg gggcccgcgc
      601 tcaaccccga catcgtggat cgcttccaga ccatctattt cgcccacatc cagcgcgagg
      661 aagcggaagc cttctccgtc gcggtggact gtctgccccc cgaggaactg gagcgcatcg
      721 gccgcgccat ggcggcgcgg cgaaacgtcg cctaccccga cgcggacgtg gtcgtgccca
      781 agtccgaggc gttgtcgcag tccgcgcgcg ccgtctgacg ccgggcaatc ttttactggc
      841 gcgttgcgcc ggagcgtact atcgtgtcca tgcgtacacc gaaccggcga tccaccatgc
      901 gcacacgtcg attggcttac gaaccgcggg cccacatccc ggccgcgatc accttcgccg
      961 cgctgacgtt cagcacgctg cgcggcgtcg atttcctggt gctcgccatc ctgctcggct
     1021 acgtcgcgct gtccacctat ctgccgcgcc cggcagcctt cagaaaagcg gcgtcgcgct
     1081 atctgtagcg cttgcgcgtt tcgtgcgtcg ccgcagaagc gaatacgcct tacacgcgca
     1141 gaatggggca atcaattcca tacttgacgt acggtgtagt gcctgagcgc ttgcgcagtc
     1201 gcgcaatcat gtaagcactg cttgggtgca ggcaattttt agcggccgtc acatcgttac
     1261 cgccgcacgc gcgcgatcat tcgccgcgat gaaacccgaa cttgtttgtc ccgccggcgc
     1321 attgcccgcg ctgaaggctg ctgtggatgc gggcgccgat tgcgtttaca tcggtctgcc
     1381 cgacaacacc aacgcccgca acttccccgg tctcaacttc gacatgacct ccgcgcgcga
     1441 aggcatccgc tatgcgcacg cggcgggccg ccaggtcttt ctcgccatca acacctatgc
     1501 gcaggcggcg gcgatggatc gctggtaccg cgctgtcgac accgcggcgg agctgggtgt
     1561 cgacgcggtg atcctcgccg atccgggcgt gatgcgttat gccgccgaga agcatccgga
     1621 cctgcgcctg catttgtccg tgcaaggctc cgcaacgagc gccgccgcga tcaacttgta
     1681 cgcgcgcagt ttcggcgttc agcgcgcggt gctgccgcgc gtgctgtcgc tgcagcaggt
     1741 agaacaggtg gtgcgcgaat cgaatgtgga aatcgaagtg ttcggcttcg gcagcctgtg
     1801 cgtgatggcg gaggggcgtt gttcgctctc ctcctatgcc acaggcgaat cgcccaacac
     1861 gcacggcgtc tgttcgccgg cgagcgcggt gcgttgggag ccgaaggacg acggcatgga
     1921 cgcgcgtctg aacggcatcc tcatcgaccg cttcgacaac gacgagcgcg ccggctatcc
     1981 gacgctgtgc aagggccgct tcgaggtggc gaacgaaacc tactacgcca tcgaagagcc
     2041 ggtcagcctc aacaccatgt cgctgttgcc cgccttggcg cagatgggcg tcgcggcgat
     2101 caagatcgaa ggccgccagc gcagtccggc ctatgtctcg caagtgactc gcgcatggcg
     2161 tgccgccatc gacgccgccg accgcaatcc cgcgcgcttc gcccctgatc cggcgtggcg
     2221 caatgcgctc gcgcgcgtgt ccgaaggtca gcaggtcacc ttcggggcgt atgaacggaa
     2281 gtggcggtga accgcctgga ttccgggtcg cgccctgacg ggtttgcccg gaatgacgaa
     2341 gaagaatcta tgcgtttatc tctcggtccc ctcctctatt actggccgcg cgacatcgtg
     2401 caggcgttct acgcacgcat ggcggaaacg gcggtcgaca ccatctatct cggcgaattg
     2461 gtctgcagtc gccggcatga gttgcgggtg gacgactggc ttgcgctggc gcgcgaactg
     2521 aaggcggccg gcaagcaggt ggtgctgtcc acgcaggcat tgatcgaatc gcccgccgac
     2581 atgcgcacgc tgcgccggct gtgcgagaac gacgagtttt tggtcgaagc caatgacgcc
     2641 agcgcgctgg gcagcatgca ggaaggcgcg gcgttcatcg ccggcccgca tctcaacgcc
     2701 tacaacggcc agaccctgca gtggtacgcc gaactgggcg cggtacgctg ggtgatgccg
     2761 gtggaagtgt cgcaggcggc actgcgcgcc atccagcggg aacgccccgc aggcatggaa
     2821 acggaagtct tcgcatgggg ccgcctaccg ctcgcattct cggcgcgctg cttcaccgcg
     2881 cgtcatcaca atctgccgcg cgacgactgc cgctatcgct gcatcgagga cccggacgga
     2941 ttgccgatgc ggacacagga aggccagccc ttcctgacgc tcaacggcac gcagacgcag
     3001 tcggccggtt attacgatct ttccgccgag ctcgacggcc tgcgcgatgc gggcgtggac
     3061 gtgctgcgca tctccccgca gtcggccggc accgcggaag tcatcgacac ctttgcgcgc
     3121 gcgatcgcgg gcacgctgcc gcccgacgaa cgcgacgaac gcctgcgcgc cgtcgccgcg
     3181 catcgctgca atggccattg gcacggacgt cccggagtcg aatatgttgc acgcccccgc
     3241 tgaacccctg cgtcgcctga tcgcacgcct gcccgcgcgc ccgccggccg tcgcacttgc
     3301 gacggcgctc aatctcgcct tgcgtcccaa actgaacgag gcgcatcgcg ccatgctgca
     3361 tggccgcgtc gtgctgatcg gtgtcaccga cgcgggcatc gagtgccgca tctcctggtc
     3421 gggcacgcgc ttcgtcgcac atgcccggca cacgccgcac gacgtggcga tccgcgcctc
     3481 ggtgcgcgat ttctatgcgc tcgccttcca tacggaagat ccggatacgc tgttctttca
     3541 acgtcgtctg cgcatcgaag gcgataccgc actcggtctc ggcatcaaaa acctgctcga
     3601 cagcatggaa ttgccgggct ggattgcggg cttacatgcg cgctggatgc aacggcaatc
     3661 gcgctatatt tgatccagcg caaagccgca gacatccggc cgactgatga tcggccggta
     3721 attcccgagt cttttgatag gaatagaaag catgactgag ccgcatttgc acatccttca
     3781 ggatcagatt tatcccttcg acgcacgcgg catcgccaag cgcttccggc acgccgcgat
     3841 cttcggcgcc ttcgacgcgc tgcgccccgg cgagatcatg cgtttcgtca acgatcacga
     3901 tccgctgccc ctgctgcagc agtttcagca gcgctacggc gagcacgtct ccatcgagta
     3961 caagcagcgc gaaccgggcg cgatcgtcat cgacttccag cacgtctgag cggagtacgg
     4021 tttggcgaac gaataccggc agcacgccgc acagggcctc accatggaaa tgctgaccac
     4081 cgatttaccg aggctgcgca tcaaggggcg ctcgctgctc cccatcgtgc agggcggcat
     4141 gggggtgggc gtttccgcac atcggctggc cggtagcgtc gcgcgccagg gcgcggtggg
     4201 cacgctttcg agcgtcgatc tgcgccgcca tcacgacgac ctcatggcgc gcacgcacaa
     4261 gtcgggcgac aaggcgctga tcgatgcggc gaacctcgaa gcgctcgatc gcgaagtgac
     4321 cgcggcgctt gcgctggccg acgggcgcgg cctggtcgcc gtgaacgtga tgcgcgccgt
     4381 ctccgagtac gcgcattacg tgcagcagtc ctgcaagagc ggtgcgcagg ccatcgtggt
     4441 gggggcgggt ctgccgctcg acctgccgga cctgacggcg gactatcccg acgtggcgct
     4501 cattccgatt ctttcggatg cgcgcggcat cgcggtgctg gtgcgcaagt ggatgcgcaa
     4561 gaaccgcctg cccgacgcga tcgtgatcga acacccgcgc tatgccggcg gccatctggg
     4621 cgccgcgaaa atcgaagacc tgaacgatcc tcgcttcgac ttcgagaacg tcattcccga
     4681 aacgcagaag ctgttcgaac aactgggcat cgaaccgatt ccgctgattt gcgcgggcgg
     4741 catcagcacg ccggaacgcg tgcgcgaatt gatcggcctg ggcgcgagcg ccgtacaact
     4801 gggcacccct ttcgcggtga ccgaggaagg cgacgcgcac atcaacttca agaaagtgct
     4861 cgccgaagcc aaacccgagg acatcgtcga attcatgagc aacgcgggcc tgcccgcacg
     4921 cgccgtgcgc acgccctggc tcgacaccta cctcaagaaa ctgtcgatcc atgaagcgcg
     4981 cgcaaaaatc cgccagtgca ccttcaagtt cgactgcctg gaagcttgcg gctttcgcga
     5041 cggcatttcc aagatcgggc agttctgcat cgaccagcgc ttgtcgctcg cggtgaaggg
     5101 cgacgtgcgc aatggtctgt agat
//
LOCUS       DATGOU010000100        15252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3403821, whole genome shotgun sequence.
ACCESSION   DATGOU010000100 DATGOU010000000
VERSION     DATGOU010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15252
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3403821"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VKZ48_06030"
     CDS             <1..471
                     /locus_tag="VKZ48_06030"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HLU77441.1"
                     /translation="LIRLDSPLLRTLRFNFEAWTNYWNQWVLGYDVQRQRTLLTRFGM
                     PTPDWQNVAMALFWTLGLAVLLLSLWLLRRVTHADPAQLAWLRFCRKLRRRGIVRRAN
                     EGPIDFAARAAHARPEHAQSIVSIARLYAEVRYGPQANSHQQAQLARMVQSFRP"
     gene            468..1463
                     /gene="mltB"
                     /locus_tag="VKZ48_06035"
     CDS             468..1463
                     /gene="mltB"
                     /locus_tag="VKZ48_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HLU77442.1"
                     /translation="MKPAPLLLAVFLYCATAAAAPAIGAPGSYLERTEVQAFIAEMSK
                     RHGFDADALRDIFAQTTQQESVLRAITPKPVGKRSWQSYRANFVSSRRIEQGVAFWHA
                     HRAALERAEQQFGVPAEIIVAILGVETHYGRAMGSFRVVEALATLAFDYPRRADYFRS
                     ELEEVLLLARESQWPPLELTGSFAGAIGIPQFMPGSIRRYAVDFDGDGRRDLRDSAAD
                     AIGSVASFLKLHGWVPGEPIAARATLEDDKAKALIDGGVKPDHPVATLRLAGARFDPD
                     LPASAPAVLIELDSTDAPPEYLVGLQNYYVITRYNRSSFYATAVWELAQALKRAM"
     gene            complement(1693..2670)
                     /gene="rpoS"
                     /locus_tag="VKZ48_06040"
     CDS             complement(1693..2670)
                     /gene="rpoS"
                     /locus_tag="VKZ48_06040"
                     /inference="COORDINATES: protein motif:HMM:NF004207.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HLU77443.1"
                     /translation="MQRTIFHEDGYEGEDVRAPDETRAEDDPQEAAELPPEFDERVSA
                     RASPEFLSDVTQIYLHEIGLKRLFDAKEELHYARRNAQGDFSARQRMIEHNLRLVVNI
                     AKGYLNRGLPLLDLIEEGNLGLMHALEKFDPERGFRFSTYATWWIRQNIERAIMNQSR
                     TIRLPVHVIKEINSALRARRHLEAAGHRDPRIEDIAHLIGKPPEEVRRVLSHNEHTAS
                     LDAPLEVDPGLSIGESIADDDSEPPEAGLQAHEIEVLVREWLGQLNDKQRNVIERRFG
                     LSGHEVLTLEHLASQLGVTRERVRQIQLEALAHLRRILSRRGLSRDALL"
     gene            complement(2740..3756)
                     /locus_tag="VKZ48_06045"
     CDS             complement(2740..3756)
                     /locus_tag="VKZ48_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HLU77444.1"
                     /translation="MNRALLGLTSLAMLVAIGLAGCASTVPAPVIERGTNHLDPRAET
                     YTVKRYDTLYSIALEHGMDWQELAAMNGITNPSTLAAGQVLRVKRQGAMVSAAPAEGV
                     QVNPVAVSSGIEVRPIEGSAVGPASSPAVGQPAAPATPGAVAGVKTGPKAYKLPYSEQ
                     NLAAVQRSDATAPAAPAAATPPAATTAEAPSTPAAEPAPAAAPATPGTAAVATVAGIN
                     WSWPTDGTLLTKYAEGSSKGVQIGGKLGDPVLASANGRVTYVGDGVRGYGNLVIVKHN
                     DEYLSVYAHNRRILVQSGQTVSKGQKIAEMGNGEGNKPLLHFEIRRFGKPVDPLQFLP
                     NRAG"
     gene            complement(3759..4394)
                     /locus_tag="VKZ48_06050"
                     /pseudo
     CDS             complement(3759..4394)
                     /locus_tag="VKZ48_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004557034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
     gene            complement(4418..5158)
                     /gene="surE"
                     /locus_tag="VKZ48_06055"
     CDS             complement(4418..5158)
                     /gene="surE"
                     /locus_tag="VKZ48_06055"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013089351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HLU77445.1"
                     /translation="MRILLSNDDGYFSPGLAALADALSDLGDLTVVAPERDRSGASNS
                     LTLDRPLIVRRSANGFLFVNGTPTDCVHLAVTGLLEELPDMIVSGINVGANMGDDTLY
                     SGTVAAATEGYLLGLPSIAVSLVGKAGSHYDTAARVARELALRFVERPIQRAVLLNVN
                     VPDLPYEALRGTEVTRLGKRHRAEGVIKTTNPRGETMYWIGAAGAAADAGPGTDFYAV
                     DQHRVSITPLQIDLTHGDQIDAVQEWLR"
     gene            complement(5452..6084)
                     /locus_tag="VKZ48_06060"
     CDS             complement(5452..6084)
                     /locus_tag="VKZ48_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HLU77446.1"
                     /translation="MTTVLILYASSLGNTKRMADAIAEGASGVAQTTVRLQTVDATSI
                     DDVWACDALVLGSPVRQGAADARVRNFIESDCERLMLSGRLATKVGAVFTVGANPHGR
                     HGDGAEMAQLGMLRTLAAGGMTIVSGPCEWRNATLRGPYWGPHARLRVREDGHEVLQK
                     GSIEAGHLHGYRIAQATLSFKHQPMARPRRVGGWLRMSGLFNGVREHMRS"
     gene            complement(6242..7912)
                     /locus_tag="VKZ48_06065"
     CDS             complement(6242..7912)
                     /locus_tag="VKZ48_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HLU77447.1"
                     /translation="MSATPAEVPVPRPRWRKPGGLGWVALITTAVIVAPILVVVGYVF
                     QPGQSSLEHLFGTVLPEYVWSTLLLMLGVAAGVISIGVTSAWLVTVYRFPGQRVLEWA
                     LVLPLAMPAYVMAYAYTDWLQYAGPVQTTLRELTGWGARDYWFPEIRSLWGAAAMFSF
                     ALYPYVYLLARTAFLEQSRSAIETARLAGYGAWGSFWRAGLPLARPAIAAGSALALME
                     TLADFGTVSYFAVQTFTTGIFRAWLSFGDIVGAAQLATCMLAFVFLLLWMERHHRGAA
                     RYQSNAPRRPLPPRQLRGRQAVVASLACCAPVVFGFFLPAGVLINLTIRERESIWVER
                     VLTLVTNSFTLAGVTALAAVLLAVLIAYATRLSKHPAVNAANRLASLGYAIPGAVIAI
                     GILIPLARFDNILADLVENAFGVDIGLLFTGTILALVYAYLVRYLAVALQTVDAGLAK
                     ITPSMDDAARSLGLGPARTLRRVHAPMLWGSMLTAGLLVFVDVMKELPATFALRPFNF
                     DTLAIEAFNLTKDERLAEAALPSLLIVAVGLLPLIIISRRMTRDRARR"
     gene            8029..9123
                     /locus_tag="VKZ48_06070"
     CDS             8029..9123
                     /locus_tag="VKZ48_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLU77448.1"
                     /translation="MPENTPMLELSDLHQSYGAHEVVHGLSMRLARGEIGCLLGPSGC
                     GKTTVLRCIAGFESVTRGEIRLNGRCVSRPGHMEPPEKRRIGMVFQDYALFPHLTVAQ
                     NIAFGLHGVPKAERATRVAEYIEAVGLAGHGDKFPHQLSGGQQQRVALARALAPQPDL
                     LLLDEPFSNLDVELRERLSLEVREIIKRTNTTAILVTHDQNEAFAIADEIGIMYGGEI
                     QQWDSAYNLYHCPGNRFIADFVGQGVFLPGYLVSDNQIQIELGTLHGAVANPCKPDCP
                     YCDDGHQVDVLLRPDDIVHDDASELQAEVVHKAFRGADILYTLRLESGRKVLALVPSH
                     HNHPLGEKIGIRLGVDHVVAFPPVHDRIAA"
     gene            complement(9127..9918)
                     /locus_tag="VKZ48_06075"
     CDS             complement(9127..9918)
                     /locus_tag="VKZ48_06075"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HLU77449.1"
                     /translation="MTASLAMAPAPLKDHTLALAVAASLLLHGVLLMWAPQWEADAPQ
                     EPPPIVARLEPAPTVQPGSAPPAPTPPQAEMPPPEPPKPRVEPKPQPKPAPRPTPKPE
                     RAVTAPPQQAAPAPAGPSMPASPALPSTAAGAEEIEDASSGTGTPDAATTAGALPQSA
                     GPVTEGDVLDAYRAAIYAAAGRHRDYPRYARARGWEGRAVVRLEVDARGQAARIAIAT
                     SSGYPVLDQSALDMVRKAQRDVPVPQALRGRPFTVEVAVLYELKE"
     gene            complement(9928..11982)
                     /locus_tag="VKZ48_06080"
     CDS             complement(9928..11982)
                     /locus_tag="VKZ48_06080"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006227346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HLU77450.1"
                     /translation="MAVSFYNLYRHNFLRAAVCVPELRVADPAFNAAQTITLMQQAEA
                     EHVAVALFPELGLTAYSCDDLFHQDALLDAASSALEQVVQASRDLQVTSVVGLPLRVD
                     GLLYNCAAVVQGGRVLGVVPKTYLPNYREFYELRQYTPGDTCTRETIDLHGQREVPFG
                     SRLLFRFAEQPLAVLHVEICEDLWAPIPPSTYGALAGATVLLNLSASNVTVGKDAYRR
                     QLVQNQSARCIAGYLYSAAGYGESTTDMAWDGHGLACEYGVLLGETKRFARRAQLLTV
                     ELDLDRLTQDRMRQNTFGVSVRRHAQALAGFRTVSIDVKLPTDDELPLNRLYERFPYV
                     PADPATRDARCAEVYDIQVHGLTKRLEAMKSDKVVIGISGGLDSTQALVVCARAMDLL
                     GLPRTNILAYTMPGFATSERTLDQARRLMMAVGVTAQEIDIRPSCLQMLKDIGHPYAE
                     GQPVYDVTFENVQAGERTSHLFRLANVHNAPVIGTSDLSEIALGWSTYGVGDHMAHYH
                     VNASVPKTLIQYLIRYVARTHLLGEEASRVLLDVLETEISPELVPGEEKDAPGQRTES
                     FVGPYELQDFHLYYTLRFGYRPSKVAFLAWNAWRDRALGVWPDIPEDKRNEYAIGDIK
                     RWLAVFLRRFFQLSQFKRSALPNAPKVGSGGSLSPRSDWRAPSDSEATAWLAELDRIP
                     DA"
     gene            complement(12168..13661)
                     /locus_tag="VKZ48_06085"
     CDS             complement(12168..13661)
                     /locus_tag="VKZ48_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLU77451.1"
                     /translation="MTTTSMPAAPVLERDERGDVCIVGAGIAGLTTAYLLVREGRNVI
                     VIDDGPVAGGASARTTAHLSNAFDDRYYEVERLHGEAGARLVAESHSAAIDTIEAIVS
                     EEGIDCDFERCDGYLVVAPGRSADELEQELQACHRAGLDDVVRVERPPLDFHDFGPAL
                     RFPRQAQFHPLKYLAQLAEAVQRRGGRIYAHTRAANLEGGERVHVETAGGAVIAADAL
                     VVATNTPISHHLAIHTKQAAYRTYVVGARVPANSVPTLLLWDTLDHYHYVRVQRDVDG
                     RDILIVGGEDHKTGQADDFDKRYARLEAWTRQRFPMVEGFEFHWSGQVLEPVDYLAFI
                     GQNPGDDDNIYVVTGDSGNGMTHGTIAGILLTDLICGHRNPWTALYDPSRITVKAAAE
                     FAKENLNVAARFVEYATPGEVKSTDDIVPGSGAVVRRGLRKIAAYRDESGMLHCHSAV
                     CTHLRCIVHWNSDELTWDCPCHGSRFDKIDGRPVNGPAQQPLGPAEI"
     gene            13814..14302
                     /locus_tag="VKZ48_06090"
     CDS             13814..14302
                     /locus_tag="VKZ48_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77452.1"
                     /translation="MANSPLTRTGETPPVVNKGHGVGSVGPSDTSDSGSDIVGGPGVG
                     QSDEFIPLDTGTTSDPDVGSGHRGNAADDIGDADMSADSDSSGTGERAAAGKDIPTNR
                     DRMPDHIEEVEQSDDVGPGPGGDQGSGPSGLESDRGSGTTGAGRDNSRTGRGNAAAPR
                     HR"
     gene            complement(14311..15249)
                     /locus_tag="VKZ48_06095"
     CDS             complement(14311..15249)
                     /locus_tag="VKZ48_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HLU77453.1"
                     /translation="MGISGWRYEGWRGGFYPEGLPQRRELEYAASLLPTIEINGSFYS
                     LQRPEYFDEWYRETPRGFLFAVKGGRYITHVRRLRDVETPLANFYASGLFNLREKLGP
                     FLWQFPPSLRYDPERFEAFFASLPRDTEAALAMAKRHSDWMKDRTQLKVDEHRALRHA
                     IEVRHESFVDPSFVDMLRRHGLAWVIADAAGKWPYKEDVTADFVYIRLHGETELYSSG
                     YDDTSLERWAGRIRDWADGREPADAQRIGSAAPTGTPRDIYCYFDNDYKVRAPFDAQS
                     LMRKLDVRWDAPEVRIDPPRRRRTRTAASDAPRPAL"
BASE COUNT         2695 a         5068 c         4960 g         2529 t
ORIGIN      
        1 ctgatccgcc tcgactcgcc gctgctgcgc acgctgcgct tcaatttcga agcctggacc
       61 aactactgga atcagtgggt gctcggctat gacgtgcagc gccagcgcac gctgctgacg
      121 cgcttcggca tgcccacacc cgactggcag aacgtcgcca tggcgctttt ctggacgctg
      181 ggcctggcgg tgttgctgtt gtcgctgtgg ctgttgcggc gcgtcacgca tgccgacccg
      241 gcgcaactgg cgtggctgcg cttctgccgc aagctgcgcc ggcgcggcat cgtgcgccgc
      301 gcgaacgagg ggcccatcga tttcgccgcg cgcgcagccc acgcacgacc cgaacatgcg
      361 caatccatcg tgtccatcgc gcggctgtat gcggaagtgc gttatggtcc gcaagccaac
      421 tcgcaccagc aggcgcaact ggcacgtatg gtgcaaagct tccggccatg aaacccgcac
      481 cccttctact cgccgtcttc ctttactgcg caaccgctgc cgccgcgcct gccatcggcg
      541 cgccgggcag ttatctggag cgaaccgaag tgcaggcctt cattgccgag atgagcaaac
      601 ggcacggctt cgacgcggac gcgctgcgcg acatcttcgc ccagaccact cagcaggaat
      661 cggtgctgcg cgccatcacg cccaagccgg tcggcaagcg ctcctggcaa agctatcgcg
      721 cgaatttcgt cagctccagg cgtatcgagc aaggcgtggc cttctggcat gctcatcggg
      781 cggcgctgga acgtgccgaa cagcagttcg gcgtgccggc cgaaatcatc gttgccatcc
      841 tcggggtgga aacgcattac ggccgcgcca tgggctcctt ccgtgtggtg gaagcgctcg
      901 ccacgctcgc tttcgactat ccgcgacgcg cggactattt ccgcagcgaa ctggaagaag
      961 tgctgctgct cgcgcgcgaa tcgcagtggc cgccgctgga actcaccggc tccttcgccg
     1021 gcgccatcgg cattccgcaa ttcatgcccg gcagcatccg ccgctacgcg gtggatttcg
     1081 acggcgacgg ccggcgcgat ctgcgcgaca gcgccgccga cgccatcggc agcgtggcga
     1141 gttttctcaa gttgcacggc tgggttcccg gcgagccgat cgccgcgcgc gccacgctcg
     1201 aagacgacaa ggcgaaggcg ctcatcgacg gcggcgtgaa gcccgaccat ccggtcgcga
     1261 ccctgcgcct ggccggcgcc cgcttcgatc ccgatctgcc cgccagcgca cccgcggtgt
     1321 tgatcgaact cgattccacc gacgcgcccc ccgaatatct ggtcggcctg cagaactact
     1381 acgtcatcac ccgctacaac cgctcgagct tctacgcgac ggcggtgtgg gaactggcgc
     1441 aggcactcaa gcgcgcgatg tgaggcagca ctgaaaagtt tgaaacacgg agcgcacgga
     1501 gggcgcggag tcaaaccatc gcaatgcgcg ctgcttcggg ccctggcatg cgtctcgcca
     1561 attcgagcgc cctgaaaatc ttgaaccaca gaggacacag agggcacaga gaaaccctct
     1621 ggcttcaatg ctttctccgt gtcctccgtg cttctctgtg atttctgtgc ttcaagcttt
     1681 tgctttaagc gcttaaagca aagcgtcacg cgacagcccg cgccggctga ggatgcgacg
     1741 caggtgcgcg agcgcttcga gctgtatctg gcgcacgcgc tcgcgcgtaa cgccgagctg
     1801 cgaggccaga tgttccagag tcagcacttc atggccgctc agtccgaaac gacgctcgat
     1861 gacattgcgc tgcttgtcgt tgagctgacc cagccattcg cgcaccagca cttcgatctc
     1921 atgcgcctgc aagcccgcct cgggcggttc gctgtcgtcg tcggcgatgg attcgccgat
     1981 ggacagtccc ggatcgacct ccagcggcgc atcgagcgaa gcggtgtgtt cgttgtggct
     2041 cagcacccgg cgcacttcct cgggcggctt gccgatgaga tgggcgatgt cctcgatgcg
     2101 cggatcgcgg tggccggcgg cctccagatg gcgccgcgcg cgcagcgcac tgttgatttc
     2161 cttgatcaca tgcaccggca gacggatggt gcgcgactgg ttcatgatgg cgcgctcgat
     2221 gttctgccgg atccaccagg tggcgtaggt ggagaagcga aatccgcgtt cgggatcgaa
     2281 cttctccagg gcgtgcatga gccccagatt gccctcttcg atcagatcga gcagcggcag
     2341 accgcgattg agataaccct tggcgatgtt caccaccagc cgcagattgt gttcgatcat
     2401 gcgctgccgc gcgctgaagt cgccctgggc gttgcgccgc gcgtaatgca gctcttcctt
     2461 cgcgtcgaac agacgcttca gtccgatctc gtgcagatag atctgggtga cgtcggacag
     2521 gaactccggt gaggcgcgtg ccgatacacg ctcgtcgaac tccggcggca gttcggcggc
     2581 ctcctgcgga tcatcctcag cgcgagtctc gtcgggcgcc cgtacgtcct cgccctcgta
     2641 cccgtcctcg tgaaagatgg tgcgctgcat agggcctcgg catcgtccca ggtcccaccg
     2701 acgggcataa gccgtgccgg ctttcgcagc gttgcgaccc tagccggccc gattcggcag
     2761 gaattgcaga ggatcgaccg gcttaccgaa ccggcggatc tcgaaatgta agagcggctt
     2821 gttgccctcg ccgttcccca tttccgcaat cttctggcct ttgctcaccg tctggccgct
     2881 ttgcaccagg atgcggcggt tgtgcgcata gaccgagagg tactcgtcgt tgtgtttgac
     2941 tatgacgagg ttgccgtagc cgcgtacgcc gtcgccgacg taggtgacgc ggccgttggc
     3001 cgaggcgagc acggggtcgc ccagcttgcc gccgatctgc acgcccttgc tgctgccttc
     3061 ggcgtacttg gtcagcaacg ttccgtccgt cggccagctc cagttgatcc cggccacggt
     3121 ggcaacggcg gcagtccccg gcgtggcggg cgctgccgca ggtgccggtt cggcggccgg
     3181 ggtcgacggt gcttcagcag tcgtcgcagc gggcggcgtg gccgccgcgg gtgcggccgg
     3241 tgcggtggcg tcgctgcgct ggacggccgc caggttctgt tcggaataag gcagcttgta
     3301 cgccttgggt ccggtcttca cgccggcgac tgcaccggga gtggccggcg cggccggctg
     3361 cccgacagcg ggcgacgaag cgggaccgac ggcgctgccc tcgatcggac ggacttcgat
     3421 gcccgagctc accgcgactg gattcacctg cacgccctcc gccggcgcgg cgctcaccat
     3481 cgcaccctgg cgcttcacgc gcagcacctg cccggcggcg agcgtgctgg gattggtgat
     3541 gccgttcatc gcggccagtt cctgccaatc catgccgtgt tccagcgcga tgctgtaaag
     3601 cgtgtcgtag cgcttcaccg tataggtctc ggcgcgcgga tcgagatggt tggtgccgcg
     3661 ctcgatcacc ggggcaggca cggtggaggc gcaaccggcc agcccgatcg cgaccagcat
     3721 cgccaacgag gtcagcccca gcagtgcgcg attcatgttc agcccttccc cagtcgcatg
     3781 ggcacgaagc gcaccgcgtc gaggcggctc tccttgaagc cgctttcggt gcgctcgacg
     3841 atcatcagcc gttgttcctt cgtcccgatg ggaatcacca gccgcccccc gatggcgagt
     3901 tgttcgagca gtgcgcgcgg catatgcgtg gccgcggcag ccaccacaat tgcatcaaag
     3961 ggtgcggcat cgggcaagcc ctgatagccg tcgccgtgcg acagccgcag attgcctacg
     4021 cgcaacggac gcagattggc gcgcgccttt tccaggagcg gcgcgatgcg ttcgacggaa
     4081 tagacctgct tggcgatctt cgccatgacc gcggcctgat agccgcaccc cgtgccgacc
     4141 tccagcacgc ggtcgagcag gcggtcggcg cgcgccgctt cgagcatgcg cgccacgaca
     4201 tagggttgcg aaatcgtctg ttcgaagccg atgggcagcg ccgagtcctc gtacgcgcgg
     4261 ctggcgagcg cctcttccac gaacaggtga cgcggcacgg cggccatggc ggcgagcacc
     4321 atttcatcgc gaatgccctg cgtgcgcaga cgctcgacca tgcggtcgcg cgtacgctgc
     4381 gaggtcatgc cgataccgcg cacgggggcc agcgggctca tcgcagccac tcctgcacgg
     4441 cgtcgatctg atcgccatgc gtgagatcga tctgcagcgg cgtgatcgac acgcgatgct
     4501 gatccacggc gtagaaatcg gtgccgggcc ccgcgtcggc ggcggcgccg gccgcaccga
     4561 tccagtacat ggtctcgccg cgcggattgg tggtcttgat cacgccttcg gcgcgatgac
     4621 gcttgcccag gcgggtgact tcggtgccgc gcagcgcctc gtacggcaga tcgggcacgt
     4681 tgacgttcag cagcacggcg cgctggatgg ggcgttcgac gaaacgcagc gcgagttcac
     4741 gcgcgacgcg cgccgcggtg tcgtaatgac tgccggcctt gcccaccagc gataccgcaa
     4801 tggacggcaa gccgagcaga tagccttcgg tggcggcggc caccgtgccc gaataaagcg
     4861 tgtcgtcgcc catgttcgcg ccgacgttga tgcccgacac gatcatgtca ggcaattcct
     4921 ccagcaggcc agtgaccgcc aggtgcacgc aatcggtcgg cgtgccgttg acgaacagga
     4981 agccgttggc cgagcggcgc acgatcagcg gccggtccag cgtcagggag ttggaggcgc
     5041 cgctgcggtc gcgttcgggc gcaaccaccg tgagatcgcc cagatcgctc agcgcatcgg
     5101 ccagtgcggc cagaccgggc gagaagtaac cgtcgtcgtt gctaagcagg atacgcatcg
     5161 tgtcagcggg aacagcaggc cgacatcgca gcgtgcgagg caaaaggcat cggaaggaac
     5221 actagagtac ggcagcgcat gttggcgcgg attataagcc acggacccgc accccactac
     5281 cgcagcggcc tgggcggcgg cgattatgtc tcggaaaggc agtcgagagc cgagagcgaa
     5341 tggttcgcct cggccgctac gcggccggct caccatgaac ggaaagtcga atttctgctt
     5401 ttactctcga ctctcggcgc tccgctctcc gctctcgacc ctcgaccgat ttcacgaccg
     5461 catgtgctcg cgcacgccgt tgaacagccc gctcatgcgc agccagccgc cgacgcgacg
     5521 cggccgcgcc atgggttgat gcttgaacga gagcgtggcc tgcgcaatgc gatagccgtg
     5581 cagatgtccg gcttcgatgg agcctttctg caatacttcg tggccgtctt cccgcacgcg
     5641 cagccgcgcg tgcggccccc aatacgggcc gcgcagtgtg gcgttgcgcc attcgcaggg
     5701 accgctgacg atggtcatgc cgcccgcggc gagcgtgcgc aacatgccca gttgcgccat
     5761 ctccgcgccg tcgccgtgac gcccgtgagg attggcgccg accgtgaaca ccgcacccac
     5821 cttggtcgcc agcctgccgc tcagcatcag acgttcgcaa tcgctttcga tgaaattgcg
     5881 cacgcgcgca tcggccgcac cctgacgcac cggcgagccg agcaccaacg catcgcaggc
     5941 ccacacatcg tcgatcgacg tggcatcgac ggtctgcaaa cggacggtgg tctgcgccac
     6001 gccgctcgcg ccttcggcaa tcgcatcggc catacgcttg gtgttgccca gactgctggc
     6061 gtagaggatc agtacggtgg tcataggcgg gcctgtcggg agcgggaatg ccggtgaacg
     6121 gctgtcggcg gcgttttatc gcaagccctg tgccgcctac cctaaaagac tggcccaaaa
     6181 gaggaaaacc gcccccgaac gcgacgcgag gcgcgtcttt gtcaaaccga gcgacaaccg
     6241 ctcagcgccg tgcgcgatcg cgcgtcatgc gccgcgaaat aatgatgagc ggcaacagac
     6301 cgaccgccac gatgagcagg gaaggcagcg ccgcctcggc aagccgctcg tccttggtga
     6361 ggttgaaggc ttcgatggcg agggtgtcga agttgaacgg ccgcaacgcg aaagttgcgg
     6421 gcagttcctt catcacatcg acgaacacca gcagacccgc cgtgagcatg ctgccccaca
     6481 gcatcggcgc gtgcacgcga cgcagcgtgc gcgccgggcc gagaccgaga ctgcgcgcgg
     6541 cgtcgtccat ggacggtgtg atctttgcca gtcccgcatc gaccgtctgc agcgcgacgg
     6601 cgagatagcg caccagatag gcgtacacta acgccagaat ggtgccggtg aacagcagac
     6661 cgatatcgac gccgaaggcg ttttcgacga gatcggccag gatgttgtcg aagcgcgcga
     6721 gcggaatcag gatgccgatg gcgatgaccg cccccggtat ggcgtagccc agactcgcga
     6781 gccgattggc ggcattgacg gccggatgct tggacagccg cgtggcatag gcaatcaaca
     6841 ccgcaagcag caccgcggca agcgccgtca cgccggcaag cgtgaagctg ttggtcacca
     6901 gcgtcagcac gcgctcgacc cagatggatt cgcgctcgcg aatcgtcagg ttgatcagca
     6961 cgcccgccgg caggaagaag ccgaatacca ccggcgcaca gcaggccagc gatgcgacga
     7021 ccgcctggcg gccgcgcagt tggcgcggcg gcaacggccg acgcggcgca ttgctctgat
     7081 agcgcgccgc gccgcggtga tgccgctcca tccacagcag caggaacacg aaggcaagca
     7141 tgcaggtggc cagttgcgcc gcgccgacga tgtcgccgaa ggacagccac gcgcggaaga
     7201 tgccggtggt gaaggtctgc acggcgaaat aggacacggt gccgaagtcg gccagcgttt
     7261 ccatcagcgc gagcgccgaa ccggcggcga tggccgggcg cgccagcggc agccccgcgc
     7321 gccagaaact gccccacgca ccataacccg ccagacgcgc ggtttcgatg gcactgcgcg
     7381 actgctccag aaatgcggtg cgcgcgagca gatagacgta aggatagagc gcgaaggaga
     7441 acatcgccgc cgccccccaa agcgagcgga tttcggggaa ccagtaatcg cgcgcgcccc
     7501 agccggtcag ttcgcgcagc gtggtctgca ccggcccggc gtattgcagc cagtcggtat
     7561 aggcgtacgc catgacgtaa gcgggcatag cgagcggcag caccagcgcc cactccagca
     7621 cacgctggcc gggaaagcga tagacggtca ccagccaagc ggaagtgacg ccgatggaaa
     7681 tcacgcccgc cgccacgccc agcatgagca gcagggtgct ccagacgtat tccggcagca
     7741 cggtgccgaa caggtgctcc agcgaggact ggccgggctg gaagacatag cccacgacca
     7801 ccaggatcgg cgcgacgatg acggccgtgg tgatcagcgc cacccagccg aggccgccgg
     7861 gtttgcgcca gcggggacgg ggaacgggta cttcggcggg tgtcgcggac atggagtggt
     7921 gcaaaaaaac aggcgcttgc gattgtatca ataatgattc ttgttactga cggcactata
     7981 atgcaggcca tgaacgccgt cgccactgcc ttgcccgccg acataccgat gcccgagaac
     8041 acaccgatgc tcgaattatc ggacctgcac cagtcctatg gcgcacacga agtggtgcac
     8101 ggactgtcga tgcggctggc acggggcgaa atcggctgcc tgctcggccc cagcggttgc
     8161 ggcaagacca cggtgcttcg ctgcatcgcg ggcttcgaaa gcgtgacgcg tggcgaaatc
     8221 cgcctcaacg ggcgctgcgt ctcgcggccc ggccacatgg agccgccgga aaagcgccgc
     8281 atcggcatgg tcttccagga ctacgcgctc tttccgcatc tcacggtcgc acaaaacatc
     8341 gccttcggcc tgcatggcgt gcccaaggcc gaacgcgcca cgcgtgtggc cgaatacatc
     8401 gaggcggtcg gtctcgccgg ccacggcgac aagtttccgc atcagttgtc cggcggccag
     8461 cagcagcgcg tcgcgctcgc ccgcgcgctg gcgccgcagc ccgatctgct gctgctcgac
     8521 gagcccttct ccaacctgga cgtggaactg cgcgagcggc tcagcctgga agtgcgcgag
     8581 atcatcaagc gcaccaacac cacggccatc ctcgtcacgc acgaccagaa cgaggccttc
     8641 gccatcgccg acgaaatcgg catcatgtac ggcggtgaaa tccagcagtg ggacagcgcg
     8701 tacaacctct atcactgccc gggcaaccgc ttcatcgccg acttcgtcgg ccagggcgtc
     8761 tttctgcccg gctatctggt gagcgacaac cagatccaga tcgaactggg cacactccac
     8821 ggcgcagtcg ccaatccgtg caagcccgat tgcccgtact gcgacgacgg ccatcaggtc
     8881 gacgtcctgc tgcgcccgga cgacatcgtt cacgacgatg cgagcgagct gcaggccgag
     8941 gtggtgcaca aggcttttcg cggcgcggac attctctaca ccctgcgcct ggaaagcggg
     9001 cgcaaggtcc tcgcgctggt acccagtcat cacaatcatc cgctgggcga gaagatcggc
     9061 atccgcctgg gcgtggatca tgtcgttgcc tttccgcccg tgcatgatcg catcgccgcc
     9121 tgagcgctac tccttcaatt cgtacagcac cgccacttcg accgtgaacg ggcgaccgcg
     9181 caacgcttgc ggcaccggca cgtcgcgctg cgccttgcgc accatgtcga gcgcgctctg
     9241 gtcgagcacc ggatagcccg aagacgtcgc gatcgcaatg cgcgcggcct gtccgcgcgc
     9301 atccacttcc agccgcacta ccgcacgccc ttcccatccg cgcgcacgcg catagcgggg
     9361 atagtcgcga tgccgtcccg ccgctgcgta gattgccgcg cgataggcat cgagcacatc
     9421 gccttcggtc accggcccgg cagattgcgg caaggcgccg gccgtcgtcg ccgcatccgg
     9481 cgtcccggta ccggacgagg cgtcttcgat ctcttcggcc ccagcagccg tcgatggtaa
     9541 ggcgggcgac gccggcatgg acggtccggc cggcgcaggg gcagcctgct gcggcggtgc
     9601 ggtgacggcg cgctcgggtt tcggggtcgg tcgcggtgcc ggtttcggct gcggcttcgg
     9661 ttcgacgcga ggcttgggcg gctcgggcgg gggcatctcc gcttgcggcg gcgtgggcgc
     9721 aggcggcgcg ctgcccggct gcaccgtcgg cgcgggctcg agtcgggcga cgatcggcgg
     9781 cggctcctgc ggcgcatcgg cttcccactg cggcgcccac atcaacagca caccgtgcaa
     9841 taggagcgag gccgcgacgg caagcgcgag cgtgtgatcc ttgagcggcg cgggcgccat
     9901 cgcgagactg gccgtcacga ccggcgctca ggcgtcggga atgcggtcga gttcagccag
     9961 ccaggccgtc gcctcgctgt cgctcggcgc acgccaatcg ctgcgcggcg acagcgaacc
    10021 gcccgagccg accttcggcg cattaggcaa cgcactgcgt ttgaattgcg aaagctggaa
    10081 gaaacggcgc agaaagacgg cgagccagcg cttgatgtcg ccgatggcgt attcgttgcg
    10141 cttgtcttcg ggaatgtccg gccacacgcc gagcgcacga tcgcgccacg cgttccaagc
    10201 gagaaaggcc accttgctcg ggcgatagcc gaagcgcaac gtgtaataaa gatgaaagtc
    10261 ctgcagctcg tagggaccga cgaagctctc ggtgcgctga cccggcgcgt ccttctcctc
    10321 gcccggcacg agttcgggac tgatctcggt ttccagcacg tcgagcagca ccctgctcgc
    10381 ttcctcaccc agcaaatgcg tgcgtgcaac gtagcggatc agatactgga tcagcgtctt
    10441 cggcacgctg gcgttcacgt ggtagtgcgc catgtggtcg cccacgccgt aggtgctcca
    10501 gccgagcgcg atttcggaaa gatcgctcgt gccgatcacc ggcgcgttgt gcacgttcgc
    10561 cagccggaac agatggctcg tccgctcgcc cgcctgcacg ttctcgaagg tgacgtcgta
    10621 caccggctgc ccttccgcgt agggatggcc gatgtccttc agcatctgca ggcagctcgg
    10681 acgaatatcg atttcctgcg ccgtaacccc gaccgccatc atgaggcgac gcgcctgatc
    10741 gagcgtgcgc tcgctcgtcg cgaagccggg catggtgtag gcgaggatgt tggtgcgcgg
    10801 caacccgagc agatccatcg cgcgagcgca cacgaccaac gcttgcgtcg agtcgaggcc
    10861 gcccgatatc ccgatgacga ccttgtcgct tttcatcgct tccaaacgct tggtcagacc
    10921 gtgcacctgg atgtcgtaca cctcggcgca gcgcgcatcg cgcgtggccg gatcggccgg
    10981 cacataagga aagcgctcgt acagccggtt gagcggcagc tcatcgtcgg tcggcagctt
    11041 gacgtcgatg ctcaccgtgc ggaaaccggc gagtgcttgc gcatgtcgac gtacgctgac
    11101 gccgaaggtg ttctgccgca tgcgatcctg cgtgaggcga tccagatcca actcgacggt
    11161 cagcaactgc gcacgccgtg caaagcgttt cgtttcgccc agcagcacgc cgtattcgca
    11221 ggcaaggccg tgaccgtccc aagccatgtc cgtggtcgat tcgccgtagc ccgcagcgct
    11281 gtagagatag cccgcaatac aacgcgccga ctggttctgc accaactggc ggcgataagc
    11341 gtccttcccc accgtgacgt tggaagcgga aagattcagc agcaccgtcg cgccggcaag
    11401 cgcgccataa gtggaaggcg gtatgggcgc ccagagatct tcacaaatct cgacgtgcag
    11461 caccgcaagc ggctgctcag cgaagcggaa caacaggcgc gaaccgaagg gcacctcacg
    11521 ctgaccgtgc agatcgatgg tctcgcgcgt gcaggtatcg cccggggtgt actgtcgcag
    11581 ttcatagaac tcgcgatagt tgggcagata cgtcttcggc actacgccga gcacgcgccc
    11641 gccctgcacc actgccgcac agttgtagag caagccgtcc acgcgcaacg gcaagccgac
    11701 gacgctcgtc acctgcaaat cgcgcgatgc ctgcaccacc tgttccagtg cgctcgaggc
    11761 cgcatcgagc aacgcatcct gatggaacag atcgtcgcag ctgtacgccg tcaatcccag
    11821 ttcgggaaag agcgcgaccg ccacatgctc cgcttccgcc tgctgcatga gcgtgatggt
    11881 ctgcgcggca ttgaaggccg gatcggcgac gcgcagttcc ggcacgcaca ctgcggcgcg
    11941 caggaagtta tggcgataga ggttgtagaa agacacggcg atcggggcgt tgcgctggag
    12001 cggcgtatct taccgtacgg cccgctaccg ctcggaactg aacgccggtg atcggtgcat
    12061 cggctcatcg gctgcgggct ggcgcccgcg tttgcgcagc gccttgtttt cttgacgaaa
    12121 aaacgcgcct ccggctacaa aaaacgagga caaggggacg cgcgcgtcta gatttccgca
    12181 ggccccaacg gttgctgcgc cggcccgttg acggggcggc cgtcgatctt gtcgaagcgc
    12241 gagccgtggc aggggcaatc ccaggtcagt tcgtcgctgt tccagtgcac gatgcagcgc
    12301 agatgcgtac agacggcgga atggcagtgc agcatgccgc tttcgtcgcg ataggcagcg
    12361 attttcctga ggccgcggcg gacgacggcg ccgctgcccg gcacgatgtc gtcggtgctt
    12421 ttcacttcac cgggcgtggc gtattcgacg aagcgtgcgg cgacgttgag attttccttg
    12481 gcgaattcgg cggccgcttt caccgtgatg cgtgaaggat cgtagagcgc ggtccaaggg
    12541 ttgcggtgac cgcagatcag gtcggtcaac aggatgccgg cgatggtgcc gtgcgtcatg
    12601 ccgttgcccg agtccccggt gacgacatag atgttgtcgt cgtcgcccgg gttctggccg
    12661 atgaacgcaa gatagtccac cggctccagc acttgccccg accagtggaa ttcgaaccct
    12721 tccaccatcg ggaagcgttg tcgcgtccac gcttccagcc gcgcatagcg cttgtcgaaa
    12781 tcgtcggcct ggccggtctt gtgatcctcg ccgccgacga tcaggatgtc acggccatcc
    12841 acatcgcgtt gcacgcgtac gtagtgatag tgatcgagcg tgtcccacag cagcagggtg
    12901 ggaaccgagt tcgcgggaac gcgcgcgccg accacatacg tgcgatacgc cgcctgcttg
    12961 gtgtggatgg ccaagtggtg gctgatcggc gtgttggtgg cgaccaccag cgcatcggcg
    13021 gcgatgacgg cgccgcccgc cgtctcgaca tggacgcgct cgccgccttc gagattcgcg
    13081 gcacgcgtat gcgcgtaaat gcgcccgccg cgccgttgaa cggcctccgc gagttgagcg
    13141 agatacttca acggatggaa ttgcgcctgc cggggaaagc gcagcgcagg gccgaagtcg
    13201 tgaaaatcga gcggcggacg ttcgacgcgc actacatcgt ccagacccgc gcgatgacaa
    13261 gcctgcaact cctgttcgag ttcatccgcc gagcggccgg gcgcgacgac gaggtagccg
    13321 tcgcagcgtt cgaaatcgca gtcgatgcct tcttccgaga cgatcgcctc gatggtatcg
    13381 atggcggcgc tatggctttc cgccaccagt cgtgcgcccg cctcgccgtg cagacgctcc
    13441 acttcgtagt agcgatcgtc gaaggcattc gacaaatgcg ccgtcgtgcg tgcggaagcg
    13501 ccgccggcga ccggaccgtc gtcgatgacg atcacgttgc gcccttcgcg caccagcaga
    13561 taggcggtcg tcagtcctgc gatgcccgcg ccgacgatgc aaacgtcccc gcgctcatcg
    13621 cgctcgagca ccggcgcggc gggcatgctc gtcgtcgtca tccacaccga ccggttcgct
    13681 cccatgtcgc tctgcatcgt cgtcgctcca gcggcctgtc caaacgtcga gcgaatgcaa
    13741 cgcctatacc cgcaagcttt cagtggcaca ggccttgcag ccttgaaggg cattcaacgc
    13801 agcaggagca accatggcga acagcccact cacgcgcacc ggcgaaaccc cgccggtggt
    13861 gaacaaggga cacggggtcg gctcggtcgg gccgagcgac acgtcggaca gcggtagcga
    13921 catcgtcggc ggaccgggcg tgggtcaatc ggatgaattc attccgctgg acaccggcac
    13981 cacttccgat ccggatgtgg gcagcggcca ccgcgggaac gccgccgacg acatcggcga
    14041 cgccgacatg agcgccgaca gcgattcatc cggcacgggg gaacgcgccg ctgcgggcaa
    14101 ggacattccc accaaccggg atcgcatgcc ggatcacatc gaggaggtgg aacagtccga
    14161 cgacgtcggg cccggccccg gcggcgatca aggtagtggc cccagtggcc tggaaagcga
    14221 tcgcggttcg ggaacgacgg gggccggacg cgacaacagc cgtaccgggc gcggcaatgc
    14281 cgcagcccca cggcacagat agatgaaggt ctaaagcgca ggtcgcgggg cgtcgctcgc
    14341 agcagtacgc gtgcgacgcc ggcgcggcgg gtcgattcgt acctcgggtg catcccagcg
    14401 cacatcgagc ttgcgcatca gcgactgcgc gtcaaaaggt gcgcgcactt tgtagtcgtt
    14461 gtcgaaatag cagtagatgt cgcgcggcgt tccggtcggt gccgcgctgc cgattcgctg
    14521 cgcgtccgca ggctcgcgac cgtcggccca atcgcggata cgccccgccc agcgctccag
    14581 gctcgtgtcg tcgtagccgc tgctgtagag ctccgtttcg ccgtgcaggc ggatgtagac
    14641 gaaatccgcg gtcacgtctt ccttgtaggg ccacttgccc gccgcatccg cgatcaccca
    14701 cgcgagtcca tgtcgccgca gcatgtcgac gaacgagggg tcgacgaagc tttcgtggcg
    14761 gacctcgatg gcatgacgca gggcgcggtg ttcgtccacc ttcaactgcg tgcgatcctt
    14821 catccagtcg ctatgccgct tcgccatggc aagcgcggcc tccgtatcgc gcggcaagct
    14881 cgcaaagaaa gcctcgaagc gctccgggtc gtaacgcagg ctcggcggaa actgccagag
    14941 gaagggcccg agtttctcgc gcaaattgaa caacccggag gcgtagaaat tcgccagggg
    15001 cgtttccacg tcgcgcagcc ggcgcacatg cgtgatatac cgcccgccct tcactgcgaa
    15061 gaggaagccc cgcggcgtct cgcgatacca ctcgtcgaag tattcggggc gctgcaggga
    15121 atagaaggag ccgttgattt cgatggtcgg cagcagcgag gcggcatatt cgagctcacg
    15181 ccgctgcggc aatccttccg ggtagaaacc gccgcgccag ccttcgtaac gccagccgga
    15241 gatgccgatg cg
//
LOCUS       DATGOU010000101         4146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_5622362, whole genome shotgun sequence.
ACCESSION   DATGOU010000101 DATGOU010000000
VERSION     DATGOU010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5622362"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..502)
                     /locus_tag="VKZ48_06100"
     CDS             complement(266..502)
                     /locus_tag="VKZ48_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77454.1"
                     /translation="MLNALAEEVSSRGGFGGARGTAGHQHTDETLRCPWCEGDVYRVR
                     RRMADRFASVLSPRQRYRCVSWSCRWEGTIRVRR"
     gene            823..1233
                     /locus_tag="VKZ48_06105"
     CDS             823..1233
                     /locus_tag="VKZ48_06105"
                     /inference="COORDINATES: protein motif:HMM:NF023125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3293 domain-containing protein"
                     /protein_id="HLU77455.1"
                     /translation="MYGSTPRERFTLRIGQREPKLAALHARKRVRCSAFITAWHPRSI
                     VRAPSLNRVAQAKLLRDLRLLGCIVVPGQGIGTDGQWREPSVLALGLRRMDAKRLALR
                     YGQNAAVVCDIRAIPQLLLVRPLLGAASQPIQAA"
     gene            1498..1588
                     /locus_tag="VKZ48_06110"
     tRNA            1498..1588
                     /locus_tag="VKZ48_06110"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1532..1534,aa:Ser,seq:gga)
     gene            complement(1676..3091)
                     /locus_tag="VKZ48_06115"
     CDS             complement(1676..3091)
                     /locus_tag="VKZ48_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLU77456.1"
                     /translation="MPDRAELLSRIARIVGPEGLVRDEAAVRAHATDWRKRYVGAPLA
                     VVMPAETAQVAALVKLCAQTGTAVVPQGGNTSLCGGATPDTSGRQLILSLARMNRIRA
                     LDPVANTLAVDAGCTLASLHEAADTAGRLFPLDVAPARLAQIGGNLSTNAGGVQVLRY
                     GNARDFVLGLEVVLASGEVWDGMRALRKDNSGYNLKHLFIGAEGTLGVITGAVLKLYP
                     KPTAKRVALVALDSPRAGLALLEHLQQRCGERLSAFELMSAVCMDLVRKHFPDSPAVF
                     DAAHPQYALIEATDTQSQAQLHVLVEDALREAVDRGIARDARIASDEPAARAYWLLRK
                     NISDAQAAEGRNIKHDISLPISCIADFLDATDAAIEQAFPGARPVTFGHLGDGNLHYN
                     IAHPPGADEAAFLARQPEVSRVVHDSVHRFGGSIAAEHGLGQYKRAEILRYKSPLEME
                     LMRTIKRTLDPHGIMNPGKVV"
     gene            complement(3112..>4146)
                     /locus_tag="VKZ48_06120"
     CDS             complement(3112..>4146)
                     /locus_tag="VKZ48_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLU77457.1"
                     /translation="DVGWVTGHSYVTYGPLACGATQVMFEGVPTYPDAGRFWKMVQDH
                     KVTVFYTAPTAIRSLIKAGHDLPKRYDLSSLRLIGSVGEPINPEAWMWYYTTVGGERC
                     PVVDTWWQTETGAHMISPLPGATPLKPGSCTLPLPGIIADVVDESGKSLPNGKGGLLV
                     IKKPWPSMLRTIWGNPERYKAGYFPKELGGFYLAGDGAARDMKGYFTIMGRIDDVLNV
                     SGHRLGTMEIESALVANTKLVAEAAVIGRPDDVTGEAIVAFVVTKGARPTGADAQRVA
                     EELREWVAKEIGPIAKPREIRFGDNLPKTRSGKIMRRLLRAIAQNEAITQDTSTLENP
                     AILEQLKQAS"
BASE COUNT          697 a         1370 c         1344 g          735 t
ORIGIN      
        1 gcttccagcg tgcggtgacg ctgctcgagc ggaccggaag gcaatgcatc gaaggaatcg
       61 ccgcgaatgg cgagcacctt gggttcgtct ttggtgactg cgaccaccac cgcgctcaga
      121 tcgacggaaa tggcgtgctg agccggctgc ccggacggtg ccgaagcggc catcattttt
      181 cgtcctgctt attgagatgg cgcctattct actcaggccg tcccccgagg cgacagccct
      241 gccgctccca gacggcctgc accgatcagc gacgaacgcg gatcgttccc tcccaacggc
      301 aactccagga cacgcagcga tagcgctgcc gcggcgacaa cacgctggcg aagcgatccg
      361 ccatgcgccg gcgaacgcga taaacatcgc cttcgcacca gggacaacgt aaggtctcgt
      421 cggtatgctg gtgtccggcg gtgccgcgcg cgccgccgaa accgccacga ctggaaactt
      481 cctccgccag cgcgttcagc acattcccga cactcgaaaa caggccgccg cccatcgcgt
      541 tatgctgtgt gtcgccccca ggatatacat tgccttttgc tgtcatggtc acttcctcct
      601 gccgccttgg cgttttccct ccgacgacat gtccggattc gcatcgcgcg gccgtcgatg
      661 attacgtttg ccgcgccggg aaatcgctgc cctgcactcc gcaagaaaga ggcccgcgcg
      721 catgcgtgca actcccattg cagactgtca ttgcagtaca ggcggactga catgggaagc
      781 gccggttcgt tactgcaatc gtatttaaca actcgctacg aggtgtacgg cagtacaccg
      841 cgcgagcgtt tcactttgcg catcggtcag cgcgaaccga agcttgccgc gctgcacgcg
      901 cgcaagcgcg tgcgctgctc tgcgttcatt acggcgtggc atccgcgcag tatcgttcga
      961 gcgccgtccc tcaaccgcgt ggcgcaagcg aagttgctgc gcgatctgcg tctgctcggc
     1021 tgcatcgtcg tacccggcca gggcatcggc acggatgggc aatggcgcga gcccagcgtt
     1081 cttgcgctcg gcctgcgacg gatggatgcc aagcggctcg cgctgcgcta cgggcagaac
     1141 gccgcggttg tctgcgacat ccgcgccatc ccgcaactgc tcctcgtgcg accgttgctg
     1201 ggggccgcat cgcaacccat ccaagcggcg taaagcagca ttccgcgccc gacgctaccg
     1261 gggctgaccg ctacattggg ctgaggtaca atccgcgcct cttgcgccga cggtgcgcaa
     1321 gacacggaga ggtgaccgag tggttgaagg tgcgaaacgc ctggaccacg agccctccgg
     1381 gcgagtgtcg gcgcagactc atgcggcttc tgccgcgtga tgtcggagcc aaagcgggtg
     1441 tgcaacacgc tgacaggcgt gccggacgtc cgcagcaagc aggttagcag taagtacgga
     1501 gaggtgaccg agtggttgaa ggtgcacgcc tggaaagcgt gtgtacggta ataccgtacc
     1561 gcgggttcga atcccgctct ctccgccaga gattcagaag cccgcgcatg cgggcttcgt
     1621 tgtctcggac gccggaaaaa acgagcctgc accaggcagg attccgcagc gcccgtcaga
     1681 ccactttccc cggattcatg atgccgtgcg gatcgagcgt ccgcttgatg gtgcgcatca
     1741 attccatttc cagcggtgac ttgtagcgca ggatttcggc gcgcttgtac tgccccaacc
     1801 cgtgttccgc ggcgatgctg ccgccgaacc ggtggacgct gtcgtgcacg acgcggctga
     1861 cttcgggctg cctggccagg aatgccgcct cgtccgcgcc cggcggatgc gcgatgttgt
     1921 aatgcagatt gccgtcgccc aggtgtccga aggtgacggg ccgtgcgccc ggaaacgcct
     1981 gctcgatggc cgcatcggtg gcatcgagaa aatcggcgat acaggaaatg ggcagcgaga
     2041 tatcgtgctt gatgttgcgc ccttccgccg cctgcgcgtc ggagatgttc ttgcgcagca
     2101 gccagtacgc ccgtgcggcc ggctcgtcgg aagcgatccg cgcatcgcgt gcgatgcccc
     2161 gatccaccgc ctcgcgcaag gcgtcttcca ccagaacgtg cagttgcgct tgcgactgcg
     2221 tgtccgtcgc ctcgatcaag gcgtattgcg ggtgcgccgc gtcgaatacg gcgggcgaat
     2281 ccgggaaatg cttgcgcacc aggtccatgc agaccgccga catgagttca aaggcggaca
     2341 ggcgctcgcc gcagcgctgt tgcagatgtt cgagcagcgc gagcccggca cggggcgaat
     2401 cgagcgccac cagcgccaca cgtttcgcag tcggcttggg atagagcttc agcaccgcgc
     2461 cggtgatcac ccccagcgtg ccctcggcgc cgatgaacag gtgcttgagg ttgtagccgc
     2521 tgttgtcctt gcgcagcgcg cgcatgccgt cccacacctc gccgctggcc agcacgactt
     2581 ccaggccgag cacaaaatcg cgcgcattgc cgtagcgcag cacctgcacg ccgccggcat
     2641 tggtggagag gttgccgccg atttgcgcca gcctggccgg cgccacgtcg aggggaaaca
     2701 gccggccggc cgtgtccgcc gcctcatgca gcgaagcgag cgtacagccg gcatcgacgg
     2761 ccagcgtatt ggcgacggga tcgagcgcgc ggatgcgatt catccgcgcg agtgaaagga
     2821 tgagttgccg gccactggtg tcgggcgtgg cgccgccgca caggctggtg ttgccgcctt
     2881 gcggaacgac cgccgtaccg gtctgggcgc aaagcttcac cagggcggca acctgcgccg
     2941 tctccgccgg catcaccacg gccaggggtg cgccgacata gcgcttgcgc cagtctgtgg
     3001 cgtgcgcccg gaccgcggcc tcgtcgcgca ccagcccttc cgggccgacg atgcgggcga
     3061 tacgggacag cagttcggcg cgatcaggca tggttggttc aggcgctgcg ttcaactcgc
     3121 ctgtttgagt tgctcgagaa tcgccgggtt ctccagggtg gacgtatcct gcgtgatcgc
     3181 ttcgttctgc gcgatggcac gcagcaaccg ccgcatgatc ttgcccgagc gggttttcgg
     3241 caggttgtcg ccgaagcgga tttcacgcgg cttggcgatc gggccgattt ccttcgccac
     3301 ccactcgcgc agttcttccg ccacccgctg cgcatccgcg ccggtcggcc gggccccctt
     3361 ggtgacgacg aaggcgacaa tcgcctcgcc ggtcacatcg tccgggcggc cgatcacggc
     3421 ggcctcggcc acgagcttgg tattcgccac cagcgccgat tcgatttcca tggtgcccag
     3481 gcgatggccg gagacgttca gcacgtcgtc gatgcggccc atgatggtga agtagccctt
     3541 catgtcgcgt gccgcaccgt cgccggcgag gtaaaaaccg ccgagttcct tgggaaagta
     3601 gccggccttg tagcgctcgg gattgcccca gatggtccgc agcatcgacg gccagggctt
     3661 cttgatcacc agcaggccgc ccttgccgtt cggcagcgac ttgccgcttt cgtccaccac
     3721 atcggcaatg atgccgggaa gcggcagagt gcaggaaccg ggcttgagcg gtgtggcgcc
     3781 cggcagcggc gaaatcatgt gtgcacccgt ttccgtctgc caccaggtat cgaccaccgg
     3841 gcagcgctcg ccgccgaccg tggtgtagta ccacatccag gcttcggggt tgatcggttc
     3901 gcccaccgac ccgatcaggc gcaggctgga cagatcgtag cgcttgggca gatcgtgtcc
     3961 ggccttgatg agcgagcgga tggcggtggg cgcggtgtaa aacaccgtca ccttgtggtc
     4021 ctgcaccatc ttccagaaac ggccggcgtc gggataagtc ggcacgcctt cgaacatcac
     4081 ctgcgtggcg ccgcaggcga gcggcccgta ggtgacgtag ctgtggccgg tcacccagcc
     4141 cacgtc
//
LOCUS       DATGOU010000102         9819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_5627829, whole genome shotgun sequence.
ACCESSION   DATGOU010000102 DATGOU010000000
VERSION     DATGOU010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9819
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5627829"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="VKZ48_06125"
     CDS             complement(<1..1258)
                     /locus_tag="VKZ48_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HLU77458.1"
                     /translation="MAAVIAKQDRRLSLAEVLDALVEDGFVSREDADALVANRRLHRG
                     VAHPLAVVAEQKWKSARPPHKLLHLEWLTEWFAGKAGLPYYHVDPLKINFAAVTELMS
                     SAYAERFKILPVEVRGREAVVATAEPYVTEWERELAQMMRVDIKRVFANPLDINRYIV
                     EFYNFAKSVKGAAGSAEKRSELSNFEQLVELGKSNRQLDANDQHIVNIVDWLWQYAFD
                     QRASDIHIEPRREIGQVRFRIDGVLHNVYQLPAPVIAAMTSRIKILGRMDVVEKRRPQ
                     DGRIKTRTADGDEVELRLSTLPTAFGEKLVMRIFDPEVLVKDFTDLGFSQEDKQRWQD
                     MTSRPHGIVLVTGPTGSGKTTTLYSTLKALATEAVNVCTIEDPIEMVEPAFNQMQVQG
                     AIDLTFAEGVRALMRQDPDIIMVGEIR"
     gene            complement(1530..3449)
                     /gene="htpG"
                     /locus_tag="VKZ48_06130"
     CDS             complement(1530..3449)
                     /gene="htpG"
                     /locus_tag="VKZ48_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HLU77459.1"
                     /translation="MAETANKETLGFQTEVKQLLHLMIHSLYSNKEIFLRELISNASD
                     AADKLRFEALAKPELYEGDGDLKIRIDFDKAARTITVSDNGIGMNRAEVVQNIGTIAK
                     SGTREFFQSLTGDQAKDAHLIGQFGVGFYSAFIVADKVTLTTRRAGETQAVRWESDGA
                     GEYTLEDVEKPARGTEIVLHLREGEDELLNDYRLKSIIRKYSDHIALPIVMKKWTWDD
                     EKKEEVPTDEDETVNQASALWARPKNDITEEQYHEFYKHVAHDFEAPLAYTHNKVEGR
                     QEYTQLLYIPARAPFDLWDRHHRRGLKLYVRRVFIMDDAEQLLPAYLRFVRGVVDSAD
                     LPLNVSREILQESKDIDAIRSGCTKRVLSLLEDLAENHADKYRTFWSQFGQVLKEGVG
                     EDYANKERIAKLLRFASTHNDTEEQSVSLADYVGRMKEGQDKIYYVTAESFLGAKHSP
                     HLEIFRKKGVEVLLLSDRVDEWVVGHLDEFEGKPLQSVAKGGLDLGKLEDEAEKKAHE
                     KQADEFKDLTDRIKKTLGERVKDVRVTSRLTSSPACLVADEHDMGANLQRILKAAGQT
                     VPNSQPILEINPEHPMVQRLKDEEKRFDDWSHLLFDQAQLAEGATLEDPAGFVKRLNE
                     LMLDLAGGGSKIWTP"
     gene            3613..6381
                     /locus_tag="VKZ48_06135"
     CDS             3613..6381
                     /locus_tag="VKZ48_06135"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HLU77460.1"
                     /translation="MHPLLRWSAALFCAALLNFVPLQAGAAPAQNKTDADTAALPAGV
                     ERVHSLAGINEYRLANGLRVLLMPDPTRDQITVNITYLVGSRHEGYGERGMAHLLEHL
                     LFKGTPKYPNPKGTLLKHGADFNGTTSYDRTNYFETFPADDKALAVMLDLEADRMVNA
                     RVSAEDLESEMTVVRNEFEAGENNPGVLLSQRISAAAYMWHNYGKTVIGTRSDIENVP
                     IERLQAFYKRYYRPDNAVLIVAGRFDEARALDRIVRTFGKIPRPKTPVPATYTEEPPQ
                     DGEREVILRRVGDVQMVGALYHIPAGSHPDYVPVDLLTEVLTAKPTGRLHKALVETGQ
                     ATFAYGYNRQLRDAASAYFGASVRLDGSLDQARQTLLRVLEGIADEPITDEEVERARI
                     KLQNDVEMLLTNTRGVALTLSEFAAMGDWRLLFWYRDQLAKVTREDVQRVALAYLRPA
                     NRTVGLFYPTDDPQRVAIPGRPDPQQMLQDFEGGADIALGEVFEPTPENIESRVIRRT
                     VEPGIKLAMLPKKTRGGTVVVQMGLHWGTPETRANRQTACSLASAMLARGTTQRTRAQ
                     LRDEMERLRATMNVGVESASISTIRDSLPGALALAAEMLRQPSFPEQEFEMLKREMIT
                     SLDVKRNDPSALASLTVNRHLNPYPPSHWNYTPTLAEQTKLIEATTLDDVRACHAELV
                     GASHAEIAVVGDFDPDAVTAQVRELFAGFSNPGPYERVVNAYHDVTGITEVLQTPDKA
                     NAVLRGGFNLALTDTHADFPALLLGSWLIGGNSDARLARRVREQEGLSYGVGAWLNAD
                     ALDPSARFNLYAIYAPQNGERVEAAIREELRKAIEQGFEQAEFDDGRQALLHARHVAR
                     NADSGLAGRLARYAYVGRTFEWDMQLEQRIAALTPAEVQDALRRHFDLSKLSIVKAGD
                     FKAVATKP"
     gene            complement(6555..7118)
                     /gene="greB"
                     /locus_tag="VKZ48_06140"
     CDS             complement(6555..7118)
                     /gene="greB"
                     /locus_tag="VKZ48_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HLU77461.1"
                     /translation="MSKAFTRQSDGEDDDLDLAPSAEIQTPAGTKNYITPAGYQRLKD
                     EFAELWEVERPRLVDIVSWAAGNGDRSENGDYQYGKKRLREIDRRLRFLSRRLEQAEV
                     VDNTRQDHDRVYFGATVTYADRAGNERTISIVGMDEVDPARGRVSWIAPVAKALLKAR
                     EGDVVTLRTPGGVEELEVLEIRYDELP"
     gene            7227..8657
                     /locus_tag="VKZ48_06145"
     CDS             7227..8657
                     /locus_tag="VKZ48_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HLU77462.1"
                     /translation="MSKNKTLALAMALALGMGAMSGAVGTARVAHAQTAGPTTDRGLP
                     DFTQLVISQGPAVVNITVTQRRPQGADQLPFRPGDPMYEFFRRFQIPIPEMQPQPRQG
                     MGSGFIISADGYILTNAHVVSEASEVTVKLTDKRELKAKVIGADVRTDVALLKVDATD
                     LPVVKIGDPEKLRVGEWVAAIGSPFGLENSVTAGIVSAKSRSLPDGSYVPFIQTDVAI
                     NPGNSGGPLFTMNGEVVGINSQIYSRTGGYMGLSFSIPIDVAMKVANDLRKFGKVERG
                     RMGVTIQSVSRELAESFGLDRPRGALVSSVEPDSPAAKAGVQPGDIILSAGGEQIEES
                     ADLPRIVGQTRPGDTLPMALWRKGKQQSIQVTVGRMPDEEQSAAGPETTPAPPGGKLG
                     IAVRPLTAEERKQANVQSGVVIERVAPGPAATAGLREGDVIIAFNNEAVNTPADLSRL
                     ADKAKGPVAALILRDNARIYVPITVK"
     gene            8763..9065
                     /locus_tag="VKZ48_06150"
     CDS             8763..9065
                     /locus_tag="VKZ48_06150"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HLU77463.1"
                     /translation="MRKLLTLFALLGALCTVAPAHAGFWADHALAVKVSTKLQFNKKL
                     LRTVIQVEAHDGNVMLSGSVPSQELIDEAVRAAKAVEGVRSVKSYLKVGPSDASGQ"
     gene            complement(9198..>9819)
                     /locus_tag="VKZ48_06155"
     CDS             complement(9198..>9819)
                     /locus_tag="VKZ48_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HLU77464.1"
                     /translation="GLRGAVRGKAVKTTLPNPALPCPRDQVQRNFNAERPNQLWVSDF
                     TYVATWRGFVYVAFVIDVFARRIVGWRAASTPRTDLVLDALEQAVYERMPERDQLIHH
                     SDRGSQYLSIRYTERLAEAGIEPSVGSVGDSYDNALAESIIGLYKTEVIRKRGPWRNL
                     EQVEFATLEWVDWFNHRRLLEPIGDRPPAEVEDEYYATLGESAMAA"
BASE COUNT         1761 a         3188 c         3070 g         1800 t
ORIGIN      
        1 cgcggatctc gcccaccatg atgatgtccg ggtcctggcg catgagcgcc cgcacgcctt
       61 cggcgaaggt cagatcgatg gcgccctgca cctgcatctg gttgaacgcg ggctcgacca
      121 tttcgatcgg atcttcgatg gtgcagacgt tgaccgcttc ggtcgccagc gccttgaggg
      181 tggaatagag cgtggtcgtc ttgcccgaac cggtcgggcc ggtgacgagg acgattccgt
      241 gcggccgcga ggtcatgtcc tgccagcgct gcttgtcctc ctgcgaaaaa ccgagatcgg
      301 tgaaatcctt caccagcact tcggggtcga aaatgcgcat caccagtttt tcgccgaagg
      361 cggtgggcag cgtggacaga cgcaactcca cctcgtcgcc gtcagccgtg cgagtcttga
      421 tgcgtccgtc ctgagggcgg cgcttttcga ccacatccat gcggccgaga atcttgatgc
      481 ggctggtcat cgccgcgatg acgggcgccg gcaactgata gacgttgtgc agcacgccat
      541 cgatgcggaa gcgcacctga ccgatttcgc gacgcggctc gatgtgaata tcgctggcgc
      601 gctgatcgaa ggcgtactgc cacagccagt cgacgatatt gacgatgtgc tggtcgttgg
      661 cgtcgagttg ccggttgctc ttgccgagtt cgaccagttg ctcgaagttc gaaagttccg
      721 agcgcttctc cgcgctgccg gctgcgccct tcaccgattt ggcgaaattg tagaactcga
      781 cgatgtaccg gttgatgtcg agcggattgg caaatacgcg cttgatgtcc acgcgcatca
      841 tctgcgcgag ttcgcgctcc cattccgtga cgtagggttc ggcggtggcg accaccgctt
      901 cgcggccccg cacctcgacc ggcagaatct tgaagcgctc ggcataagcg ctcgacatca
      961 gctcggtcac cgccgcgaaa ttgatcttga gcggatcgac gtggtagtac ggcagtcccg
     1021 ccttgccggc aaaccattcg gtcagccact ccaggtgcag cagcttgtgc ggcggccggg
     1081 ccgatttcca cttctgctcg gcgacgaccg ccagcggatg cgccacgccg cgatggaggc
     1141 ggcgattggc gacgagcgca tcggcgtcct cacgcgagac gaagccgtcc tcgacgaggg
     1201 cgtccagcac ttccgccagc gacagccggc gatcctgttt ggcgatgaca gcggccatga
     1261 cctgcgcgta ctgatgggaa taccggggag tatgcgcgag cccgggacga ggcccaacgt
     1321 aatagttctc gcaaatgccc cgcatttcgg gggatgccga ccgtgattcg cccacgccgc
     1381 gcaaaggacg gctcgatcat ccagctgccg gaaggagcga cgcgcagcca taaagtcctt
     1441 taaccaccaa gacaccaagc tcgccaagaa acaccaagaa aaccagtcgg aagcttggtg
     1501 tcttggtgcc ttggtggttc gagatttttc tagggcgtcc agatcttcga gccgccgccc
     1561 gccagatcga gcatcagttc gttcagccgc ttgacgaagc ccgcggggtc ttccagcgtg
     1621 gcgccctccg ccagttgcgc ctggtcgaac agcagatgcg accagtcgtc gaagcgcttc
     1681 tcctcgtcct tgaggcgttg caccatcggg tgctcgggat tgatttccag gatgggctgc
     1741 gagttcggca ccgtctgccc cgcggccttg aggatgcgct gcagattggc gcccatatcg
     1801 tgttcgtcgg ccaccaggca ggccggcgac gaggtgaggc gcgaggtgac gcgcacatcc
     1861 ttgacgcgct cgcccagcgt cttcttgatc ctgtccgtca gatccttgaa ctcgtccgcc
     1921 tgcttttcgt gcgccttctt ctcggcttcg tcttccagct tgccgagatc gaggccgccc
     1981 ttggccaccg actgcagggg cttgccttcg aactcgtcca ggtggccgac cacccattcg
     2041 tcgacgcggt cggacagcag cagcacttcc acgcccttct tgcggaagat ttccagatgc
     2101 gggctgtgct tcgcgccgag gaaggattcg gcggtgacgt agtagatctt gtcctgcccc
     2161 tccttcatgc ggccgacgta gtcggccagc gagacactct gttcctcggt gtcgttgtgc
     2221 gtcgaagcga agcgaagcaa cttggcgatg cgctccttgt tggcgtagtc ctcacccacg
     2281 ccctccttca gcacctgacc gaactgcgac cagaaggtcc ggtacttgtc ggcatggttt
     2341 tcggcgagat cctccagcaa cgacagcacg cgcttggtgc agccggagcg gatcgcgtcg
     2401 atgtccttgc tctcctgcaa aatttcgcgc gagacgttga gcggcagatc ggccgaatcg
     2461 accaccccgc gcacgaagcg caggtaggcg ggcagcaact gctcggcgtc gtccatgatg
     2521 aacacgcggc gcacatagag cttcaggccg cggcgatgat gccgatccca cagatcgaag
     2581 ggcgcgcgtg ccgggatgta gagcagttgc gtgtattcct ggcggccctc gaccttgttg
     2641 tgcgtgtagg caagcggcgc ctcgaagtcg tgcgccacat gcttgtagaa ctcgtgatac
     2701 tgctcttcgg tgatgtcgtt cttgggccgc gcccagagcg ccgatgcctg gttgacggtc
     2761 tcgtcctcgt cggtgggcac ctcttccttc ttctcgtcgt cccacgtcca tttcttcatc
     2821 acgatcggca gcgcgatgtg atcggaatac ttgcggatga tggacttcag gcgatagtcg
     2881 ttgagcagtt cgtcctcgcc ctcgcgcagg tgcagcacga tctcggtgcc gcgcgcgggc
     2941 ttttcgacgt cttccagtgt gtattcgccg gcgccgtccg actcccagcg cacggcctgc
     3001 gtctcgcctg cgcgtcgtgt ggtgagcgtc accttgtcgg ccacgatgaa ggccgaatag
     3061 aaaccgacgc cgaactggcc gatgagatgg gcgtccttgg cttgatcgcc cgtcagcgac
     3121 tggaagaact cgcgcgtgcc cgacttggcg atggtgccga tgttctggac cacttcggcg
     3181 cggttcatac cgatgccgtt gtccgaaacg gtgatggtgc gtgccgcctt gtcgaagtcg
     3241 atgcggatct tgaggtcgcc gtctccctcg tagagttccg gtttggcaag cgcctcgaag
     3301 cgcagcttgt cggccgcatc cgaagcgttg gaaatcaact cccgcaggaa aatctccttg
     3361 ttgctgtaca aggagtggat catcaggtgc agtaactgct tgacctcggt ctggaatccc
     3421 agggtttcct tattggcggt ttcggccatg ggctttcgtc ctctgtgtgt ataactttcg
     3481 tgcgcggata tgggggcggc cgcgaccggt tcaagccccc gtccgccgta ccgggaactt
     3541 cccccgggcg ggcgcagtct ggtcccctac aataaccgct cctccgcccc aggtccgatc
     3601 cgaaggaaat tcatgcatcc gctgttgcgc tggtccgccg ccctgttttg cgcggcgctg
     3661 ctcaatttcg tgcccttgca ggccggggcc gccccggccc agaacaagac cgacgccgac
     3721 accgccgccc tgccggccgg cgtcgagcgc gtccactcgc tggcgggtat caacgaatac
     3781 cgactcgcca acggcctccg ggtgctgctg atgcccgatc ccacgcgcga ccagatcacg
     3841 gtaaacatca cctatctggt cggctctcgg cacgagggct acggcgagcg cggcatggct
     3901 cacctgctgg aacacctgct gttcaagggg acgcccaaat acccgaaccc caagggcacc
     3961 ctgctcaagc acggcgcgga tttcaacggc accacctcct acgaccgcac caattatttc
     4021 gagaccttcc ccgccgacga caaggcgctg gcggtgatgc tcgacctgga agccgaccgc
     4081 atggtcaacg cgcgcgtctc cgcggaggat ctggaaagcg aaatgaccgt ggtgcgcaac
     4141 gagttcgaag ccggcgagaa caatcccggc gtgctgctca gccagcgcat cagcgccgcc
     4201 gcttacatgt ggcacaacta cggcaagacc gtgatcggca cgcgcagcga tatcgagaac
     4261 gtgccgatcg agcgcctgca ggccttctac aagcgttatt accggccgga caacgcggtg
     4321 ctgatcgtcg ccggacgctt cgacgaagcg cgcgcgctcg accggatcgt ccgcacgttc
     4381 ggcaagattc cgcggccgaa aacgcccgtg cccgccacct ataccgaaga gccgccgcag
     4441 gacggcgaac gcgaagtgat cctgcgccgg gtgggcgacg tgcagatggt gggcgcgctg
     4501 tatcacatcc ccgccggttc gcatccggac tacgtgccgg tcgatctcct caccgaggtg
     4561 ctcacggcca aaccgaccgg cagattacac aaggcactgg tggaaaccgg tcaggcgacc
     4621 ttcgcctacg gctacaaccg tcagttgcgc gacgccgcgt ctgcctattt cggcgcaagc
     4681 gtgcgcctcg acggttccct cgatcaggcg cggcagaccc tgctgcgcgt gctggaaggc
     4741 atcgccgacg agcccatcac cgacgaggaa gtggaacgcg cgcgcatcaa gctgcagaac
     4801 gacgtggaga tgctgcttac caatacgcgc ggcgtggcac tgacgctgtc cgagttcgcc
     4861 gccatgggcg actggcggct gctgttctgg taccgcgatc aactggccaa ggtgacgcgt
     4921 gaagacgtgc agcgggtggc gctcgcctat ctgcgcccgg ccaaccgcac cgtcggcctg
     4981 ttctatccga ccgacgatcc gcagcgcgtg gcgatacccg ggcggccgga cccgcagcag
     5041 atgctgcagg acttcgaagg cggcgccgac atcgcgctgg gcgaagtctt cgaacccacg
     5101 ccggagaata tcgagtcgcg cgtcatccgg cgcaccgtcg aacccggcat caaactggcg
     5161 atgctgccga agaaaacccg cggcggtacc gtcgtcgtac agatgggcct gcattggggc
     5221 acgccagaga cgcgcgccaa tcgccagacg gcctgctcgc tggcgagcgc catgcttgcg
     5281 cgcggcacga cgcaacgcac acgtgcacaa ttgcgcgacg agatggaacg cctgcgtgcc
     5341 acgatgaacg tcggtgtcga aagcgcttcg atcagcacca tccgcgacag cctgcccggc
     5401 gcgctcgcac tggccgccga gatgctgcgt cagccgagtt tccccgagca ggaattcgaa
     5461 atgctgaagc gcgagatgat caccagcctg gacgtgaaac ggaacgatcc gtcggcgctg
     5521 gcgagtctca ccgtgaaccg gcatctcaac ccctacccgc cttcgcactg gaactacacg
     5581 cccacgctgg cggagcagac caagctcatc gaggctacga cgctggacga cgtccgcgca
     5641 tgtcatgcgg agttggtggg cgcgtcgcat gcggaaatcg ccgtggtcgg cgatttcgat
     5701 cccgatgccg tcacggcgca agtgcgcgaa ctgttcgccg gcttctcgaa ccccggccct
     5761 tacgaacgcg tggtcaacgc ctatcacgac gtcaccggca tcaccgaagt gctgcagacg
     5821 ccggacaagg ccaacgccgt cctgcgcggc ggcttcaatc tggcgttgac cgacacgcac
     5881 gcggatttcc ctgcactgct gctcggcagt tggctcatcg gcggcaactc cgacgcgcgt
     5941 ctggcccggc gcgtgcgcga gcaggaaggc ctgtcctacg gtgtcggcgc atggctcaac
     6001 gccgatgcgc tcgacccttc cgcgcgcttc aacctgtacg ccatctacgc gccgcaaaac
     6061 ggcgagcgtg tcgaggctgc gatacgcgag gaactgcgca aggccatcga gcagggcttc
     6121 gagcaggcgg aattcgacga cggccgtcag gcgctgctgc acgcgcggca tgtggcgcgc
     6181 aatgccgatt ccgggctggc cggacggctg gcacgttacg cttatgtcgg ccgcaccttc
     6241 gaatgggata tgcaactgga gcagcgcatc gccgcgctga cgcccgccga agtgcaggat
     6301 gcgctgcggc ggcatttcga tttgagcaag ctgtcgatcg tgaaggccgg cgatttcaag
     6361 gcggttgcga ctaaaccctg aaaaccttga accaccaaga caccaaggcg ccaagaaaaa
     6421 ctatgtgcac agggaggctt tgagccttct acggggcttg ccggcgcgcg ccgctatgcc
     6481 tgcgccggaa tggcggcctt ggtgacttct tggcgtcttg gtgccttggt ggttaagcat
     6541 ctgcttcgac tgcgctacgg caactcgtcg tagcggattt cgagcacttc cagttcttcc
     6601 acgccgccgg gcgtacgcag cgtcacgacg tcgccttccc gcgccttgag cagcgccttg
     6661 gccacgggtg cgatccacga gacgcggccg cgtgccggat cgacctcgtc catgccgacg
     6721 atgctgatgg tgcgctcgtt gcccgcgcga tcggcgtagg tcacggtggc gccgaaatag
     6781 acgcggtcgt gatcctgcct ggtgttgtcc accacctcgg cctgctcgag gcgccgggac
     6841 agaaagcgca ggcgccggtc gatttcgcgc agccgcttct tgccgtactg gtaatcgccg
     6901 ttctccgagc ggtcaccgtt gccggccgcc caggagacga tgtccaccag ccgggggcgt
     6961 tcgacttccc acaactcggc gaactcgtcc ttgagacgct ggtagcccgc cggggtgatg
     7021 taatttttcg tgccggcggg cgtctggatt tcggcggacg gagcgaggtc caggtcgtcg
     7081 tcctcgccgt cgctttggcg ggtaaaggct ttactcatac tcggggcgct atgatagcgg
     7141 cttccgtagg gttcgacgcc gcatcgggga accagtttcc ccctattcct gccaaagacc
     7201 tacatccaag acctaggagg aactcgatgt cgaagaacaa gacgctggcc ctggccatgg
     7261 cgcttgcgct tggaatgggc gccatgagcg gcgccgtcgg caccgcccgc gtggctcatg
     7321 cccagacggc cggccccacg acagaccgcg gtctgccgga tttcacgcag ctcgtcatct
     7381 cgcaaggccc ggcagtggtg aacatcaccg tgacgcaacg ccgcccccag ggcgccgatc
     7441 agttgccctt ccgccccggc gatccgatgt acgagttctt ccgccgcttc cagatcccca
     7501 ttccggaaat gcagccgcag ccgcgtcagg gcatgggttc gggcttcatc atcagcgcgg
     7561 acggttacat cctcaccaac gcacacgtgg tctccgaagc gagcgaagtc accgtcaagt
     7621 tgaccgacaa gcgcgagttg aaagcgaaag tcatcggcgc tgacgtgcgt accgacgtgg
     7681 ccctgctcaa ggtggatgcc accgatctcc ccgtggtgaa aatcggcgat ccggaaaaac
     7741 tgcgcgtagg cgaatgggtg gcggccatcg gttcgccctt cggtctggag aacagcgtca
     7801 ccgccgggat cgtcagcgcc aagagccgct ccctgcccga cggcagttac gtgcctttca
     7861 ttcaaaccga tgtggccatc aatccgggca actccggcgg cccgctgttc accatgaacg
     7921 gcgaagtggt cggcatcaac tcgcagatct acagccgtac cggcggctac atgggattgt
     7981 cgttctcgat tcccatcgac gtggcgatga aagtggccaa tgacctgcgc aagttcggca
     8041 aggtcgagcg cggccgcatg ggcgtcacca ttcagagcgt gagccgcgaa ctggccgaat
     8101 ccttcggcct ggaccgtccg cgcggcgcgc tggtcagttc ggtggaaccg gacagccccg
     8161 ccgccaaggc aggcgtgcaa ccgggcgaca tcattctttc ggccggcggc gaacagatcg
     8221 aggaatcggc tgatctgccg cgtatcgtcg gtcaaacccg tccgggcgac acgctgccga
     8281 tggcgttgtg gcgcaagggc aagcagcaaa gcatccaggt gacggtcggt cgcatgcccg
     8341 atgaagagca gtcggccgcc ggtccggaaa cgacgcctgc gccgcccggc ggcaaactcg
     8401 gcatcgccgt acgtcctctg accgccgagg agcgcaagca ggccaatgtg caaagcggcg
     8461 tggtcatcga acgcgtcgcg ccgggccccg cggccaccgc gggattgcgt gaaggcgacg
     8521 tcatcattgc gttcaacaac gaagcagtga acacaccggc ggacctgagc cggctggcgg
     8581 acaaggccaa aggaccggtg gccgcgctga tcctgcgcga caacgcacgc atctacgtgc
     8641 ccattacggt caagtaaagc ggccttccgt cgcatttcag aacccgcccc ccgcaagggg
     8701 cgggtttttt atgggcacga ccgccgctag aatggggcga cgctgactac cggatcccgg
     8761 ccatgcgcaa actgctcacc ctgttcgcac tgctcggcgc cctctgtacc gtcgcaccgg
     8821 cgcacgccgg cttctgggcc gatcacgccc tggcggtcaa agtctccacc aaactgcagt
     8881 tcaacaagaa gttgctgcgc acagtcatcc aggtcgaagc gcacgacggc aacgtgatgt
     8941 tgagcggcag cgtgccgagt caggagctca ttgacgaagc ggtgcgcgcc gccaaggcgg
     9001 tcgaaggggt acgcagcgtg aagagttatc tgaaggtggg gccgagcgac gcatcgggcc
     9061 agtagccgcc aatccttgtt gaagcgggcc gccgatcggg cggcccgctt caaggccctt
     9121 agctaagcgt catgccaccg ccggaaatgc cttcgaactg aaccgccccg actttatcgg
     9181 aggctgtttc gtttgagtca cgccgccatg gcggactcgc caagggttgc atagtactcg
     9241 tcttcaactt ccgctggcgg tcggtcaccg atcggttcga gcaatcgccg atggttgaac
     9301 cagtctaccc attccagcgt cgcgaactcg acctgctcca ggtttcgcca aggtccacgt
     9361 ttacggatca cttcggtctt gtaaaggccg atgatggatt ccgccaacgc attgtcgtaa
     9421 gagtcaccga cgctgccaac ggacggttcg atgccggctt cggcaagccg ctcggtgtac
     9481 cggatcgaca ggtattgcga accgcgatcg ctgtgatgga tcagttgatc ccgttccggc
     9541 attcgttcgt acacggcctg ttccaatgca tcgagaacca gatcggtacg cggcgtactc
     9601 gcggcccgcc agccgacgat gcgccgggcg aagacgtcaa tcacgaaggc gacatagaca
     9661 aagccacgcc aggtcgcgac gtaggtgaag tcagagaccc acagctggtt aggccgctcg
     9721 gcgttgaagt tgcgttgcac ctgatcgcgc ggacatggca gtgccgggtt cggcagcgtg
     9781 gttttaaccg ccttgccgcg taccgctccc cgcagtccc
//
LOCUS       DATGOU010000103         4520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_5193846, whole genome shotgun sequence.
ACCESSION   DATGOU010000103 DATGOU010000000
VERSION     DATGOU010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5193846"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..211)
                     /locus_tag="VKZ48_06160"
     tRNA            complement(135..211)
                     /locus_tag="VKZ48_06160"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(175..177),aa:Met,seq:cat)
     gene            complement(293..1360)
                     /locus_tag="VKZ48_06165"
     CDS             complement(293..1360)
                     /locus_tag="VKZ48_06165"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HLU77465.1"
                     /translation="MESRPIIWKGRGATGNPQGRFERDERVVDEGAYWETPERPPETQ
                     VTLRQARSIIARNDSPDIPFSQSINPYQGCEHGCIYCYARPSHSYLGLSPGLDFETRL
                     YAKENAADLLRQELSRPGYRCDVIVIGGNTDPYQPVERELRITRSILEVCAEFNQPVG
                     IITKSALIERDLDLLAPMAAKGLARAFVSVTTLDAELARKLEPRAAAPYRRIRALRTL
                     TEAGVPTGVMVAPIIPFLNDKDLEAILDAARDAGVTQAGYVLLRLPHEVKDIFQAWLT
                     EHYPLRADHIMARVRDMRGGKEYDSAWRERQTGSGIYAELLRQRFDQTCRRLGINRGA
                     MAGLVTTRFKVPGSAEQLGLF"
     gene            complement(1411..2619)
                     /locus_tag="VKZ48_06170"
     CDS             complement(1411..2619)
                     /locus_tag="VKZ48_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HLU77466.1"
                     /translation="MSAKPLAGLKVLELGQLIAGPFAGKTFAEFGAEVIKIEPPASDK
                     GEGGDPLRTWRKLHNGTSLWWHVIARSKKSVTANLRTSEGQEIVRRLARDVDVVIENF
                     RPGTLEKWGLGYDVLSKDNPGLVMLRLSGFGQTGPYKDQAGFGAVGESMGGMRYVTGF
                     PDRPPVRMNLSLGDALASLHGVIGVLMALRHRDVNGGKGQVVDVALYEAVFNMMESTL
                     PEYDMYGMVRERTGTNLTGIVPSNTYLTKDGAHVVIGGNGDSIFKRLMHAIGRPDMAE
                     DPSLADNAGRAKRADELDAVIGEWCISHDTDYVLRVLNEAQVPNGKIYSIADIVKDPQ
                     FRAREMIARARLPDGSEVAVPGVVPKLTDTPGQVGECGPALGQHTDEVLAALGYDEAA
                     RQSLRERKII"
     gene            complement(2616..3215)
                     /gene="recR"
                     /locus_tag="VKZ48_06175"
     CDS             complement(2616..3215)
                     /gene="recR"
                     /locus_tag="VKZ48_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HLU77467.1"
                     /translation="MAASSNSLDDLIEALRCLPGVGPKSAQRMAYHLLQYDRGGAQRL
                     VLSLDRALQNIRRCAKCNTFTEDEICALCASSKRDASLLCVVETPADQAVMEQTLAYQ
                     GLYFVLMGRLSPLDGVGPKEIGLEALQRRVSDGLVKEVILATNFTNEGEATAHYIHEM
                     LQDRNIKVTRIARGVPVGGELEYVDLGTLAQAFYERRQA"
     gene            complement(3217..3543)
                     /locus_tag="VKZ48_06180"
     CDS             complement(3217..3543)
                     /locus_tag="VKZ48_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HLU77468.1"
                     /translation="MMKGQLAGLMKQAQQMQENMRKVQEQLATIEVEGQSGAGMVKVV
                     MTCKHDVKRVTIDPSLLADDKEMLEDLVAAAVNDAVRRVEATVQEKMSGFTAGMGLPP
                     GFKMPF"
     gene            complement(3540..>4520)
                     /locus_tag="VKZ48_06185"
     CDS             complement(3540..>4520)
                     /locus_tag="VKZ48_06185"
                     /inference="COORDINATES: protein motif:HMM:NF023591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HLU77469.1"
                     /translation="AQSAGGSMRDALSLLDQAIAYSSGQVTEEAVRAMLGTVDTGYLF
                     DLLEALGDNDAARMIALAGEMESRSIAFDGALIELGTLLHRLALAQMAEDALPEDLSG
                     RDRIVALARRFDPESVQLYYQIVIQARHDLPLAPDEYAGFTMALMRMAAFAPERASTA
                     SSSAGGTPSKRPTPVATPRPAAGPAAVAPARREPAAAAEGDWPTLVGTLKLTGLAKQL
                     AERCEMVARNGDKFDLRVSNESRALATAASIDRLKSALQDAIGRPVRVDVQVGETRGA
                     SVAAIAETDRRARQDRASQQMNNDPFVRELVNSLDATIESVEPLQQGPQS"
BASE COUNT          791 a         1494 c         1374 g          861 t
ORIGIN      
        1 atgagcacca agtaatgttc gagataacca ccaacaccta agcatcagcg gcccgtacac
       61 caacaccacc cagcacccac acctatcggc tgttcagttt ttaaagagcg atgtcacacc
      121 agttcttttg gagctggttg cgggggcagg atttgaacct acgaccttcg ggttatgagc
      181 ccgacgagct gccagactgc tccaccccgc gtccgtgaca gaagcggaat tatggggaat
      241 cgcccgccct ctgtcaactc catttggagt aaatccggag ggaagaacgc gctcaaaaca
      301 atccgagttg ctccgcgcta ccgggcactt tgaaacgcgt cgtcaccagc ccggccattg
      361 cgccccggtt aatccccaag cgccgacaag tttggtcaaa gcgctgcctc aaaagttccg
      421 cgtatatgcc gctgcccgtt tgccgctcgc gccacgccga gtcgtactcc ttcccgccgc
      481 gcatgtcacg gacccgggcc atgatgtgat ccgcccgcaa ggggtagtgc tccgtcagcc
      541 acgcctggaa aatatccttc acttcatgcg gcaggcggag caggacatag cccgcctgcg
      601 tcaccccggc gtcccgcgcc gcgtcgagaa tcgcctccag atccttgtcg ttgaggaacg
      661 ggatgatcgg cgccaccatg acgccggtcg gcacgcccgc ctcggtcagc gtccgcaggg
      721 cgcggatgcg ccgatacggt gccgccgcgc ggggttcgag ttttcgcgcc agctcagcat
      781 ccagggttgt caccgacacg aaagcgcgcg ccaggccttt ggccgccatc ggcgcgagca
      841 ggtcgaggtc gcgctcgatc agtgctgatt tggtaatgat gccgaccggc tggttgaatt
      901 cggcacagac ctcgagaatg ctgcgcgtga tgcgcaattc ccgctcgacg ggctgataag
      961 gatcggtatt cccgccgatg acgatcacat cgcaccgata gcccggccgg gacagttcct
     1021 ggcgcagcaa gtccgccgca ttctccttgg catacagccg ggtttcgaag tcgaggccgg
     1081 gcgacaagcc cagataggaa tggctcggcc gcgcgtagca gtagatgcag ccgtgctcgc
     1141 agccctgata gggattgatc gactggctga acgggatgtc gggcgagtcg ttgcgtgcaa
     1201 tgatcgacct ggcctggcgc agcgtcacct gcgtttcggg cgggcgctcg ggcgtctccc
     1261 aataggcgcc ttcgtccacg acgcgctcgt cgcgctcgaa gcgcccttgc ggatttccgg
     1321 ttgcgccgcg gcccttccag ataatgggac gtgattccat acagctctcg aaactactgt
     1381 atggaagtat agttgttttc ggcgtgcgcg ctagatgatc ttgcgttcgc gcagcgactg
     1441 cctggcggct tcgtcgtaac cgagcgcagc cagcacttca tcggtgtgct gtccgagcgc
     1501 cggaccgcac tcgcccacct ggccgggcgt atcggtgagc ttggggacga cccccggtac
     1561 cgcgacctcg ctgccgtccg ggagccgggc gcgggcaatc atttcgcgcg cccggaactg
     1621 cgggtccttc acgatatcgg cgatgctgta aatcttgccg ttcggcacct gcgcctcgtt
     1681 gagcacgcgc aatacatagt cggtgtcatg actgatgcac cactcgccga tcacagcatc
     1741 cagttcatcc gcgcgcttgg cccgaccggc attgtcggca aggctcgggt cctccgccat
     1801 atccggccgg ccgatggcat gcatcagccg cttgaaaatg ctgtcgccgt tgccgccgat
     1861 gacgacatgc gcgccgtcct tggtgagata ggtgttggag ggcacgatgc cggtgagatt
     1921 cgtgccggtc cgttcgcgca ccatgccgta catatcgtac tcgggcagcg tgctttccat
     1981 catgttgaac acggcttcgt agagcgcaac gtcgaccact tgccctttcc cgccgttcac
     2041 gtcgcgatgg cgcaaagcca tcaagacgcc gatgacacca tgcagggacg cgagcgcatc
     2101 gccgagtgaa aggttcatcc gcaccggcgg gcggtcgggg aaaccggtga catagcgcat
     2161 gccgcccatg gactcgccca cggcgccgaa gcccgcctga tccttgtacg gcccggtctg
     2221 accgaagccg gacaggcgca gcatgacgag tccgggattg tccttgctca gcacgtcgta
     2281 gccgagcccc catttctcca gtgtgcccgg ccggaaattc tcgattacca cgtccacgtc
     2341 gcgcgccagt cggcgcacga tttcctggcc ttcagaggtg cgcagattgg ccgtcaccga
     2401 cttcttgctg cgcgcgatca catgccacca cagcgaggtg ccgttatgca gcttgcgcca
     2461 ggtgcgcagg ggatcaccgc cctcgccctt gtccgacgcc ggcggctcga tcttgatcac
     2521 ttccgcgccg aattcggcaa aggtcttgcc ggcgaaggga ccggcgatga gttgcccgag
     2581 ttcgagcacc ttcaatccgg cgagcggctt ggcgctcatg cctgcctccg ttcgtagaac
     2641 gcctgcgcca gggtgccgag atcgacgtat tcgagttcgc cgccgacggg caccccacgg
     2701 gcgatgcgcg tgactttgat gttccggtcc tgcagcattt cgtggatgta atgcgccgtc
     2761 gcctcgcctt cgttggtgaa attggtggcg agaatcacct ctttcaccag tccgtcactg
     2821 acgcgacgct gcaacgcctc cagcccgatt tccttcgggc cgacgccgtc caacggcgac
     2881 aaccgcccca tgagcacgaa gtaaagcccc tgataggcaa gcgtctgttc catcaccgcc
     2941 tgatcggccg gtgtctccac cacgcaaaga agcgaagcat cgcgcttgga cgacgcgcag
     3001 agtgcgcaga tttcgtcttc ggtgaacgta ttgcacttcg cgcaacggcg aatgttctgg
     3061 agcgcacggt cgagcgacaa caccagacgt tgcgcgccgc cccggtcgta ttgcagcagg
     3121 tgataagcca tccgctgcgc ggatttcggc ccgacgccgg gcagacaacg caatgcctcg
     3181 atcaggtcgt cgagactgtt ggaagatgct gccaagtcag aacggcatct tgaagccggg
     3241 cggtaacccc atgccggcgg tgaagcccga catcttttcc tgcacggtgg cctcgacgcg
     3301 acgcaccgca tcgttcacgg ctgcggcgac gaggtcttcc agcatttcct tgtcgtcggc
     3361 gagaagcgaa ggatcgatgg tgacgcgctt cacgtcatgc ttgcaggtca tgacgacctt
     3421 caccatgccg gcgccggact ggccttccac ttcgatggtc gcaagctgtt cctgcacctt
     3481 gcgcatgttt tcctgcatct gctgagcctg tttcatcagg ccagcaagtt gtcccttcat
     3541 catgactgcg gtccttgttg cagcggttcc acggattcaa tcgtcgcgtc gagactgttc
     3601 accagttcgc gcacgaacgg atcgttgttc atctgctggc ttgcgcgatc ctggcgcgca
     3661 cgtcgatcgg tctcggcaat ggcggcgacg ctcgcgccgc gcgtttcgcc cacctgcacg
     3721 tcgacccgca ccgggcggcc gatcgcatcc tgcaaggcgc tcttgagccg gtcgatggag
     3781 gcggccgtcg ccagggcgcg cgattcgtta ctcacgcgca aatcgaattt gtcgccgttc
     3841 cgcgccacca tttcgcagcg ctcggcaagc tgcttcgcca atccggtcag tttcagcgtc
     3901 ccgaccaatg tcggccagtc gccttccgcc gcagcagccg gctcgcgccg ggcaggcgcc
     3961 acggctgcgg gtccggccgc gggccggggc gttgccaccg gggtcgggcg tttggacggc
     4021 gtaccgccgg ccgatgacga cgctgtcgac gcccgctccg gcgcgaacgc cgccatgcgc
     4081 atgagcgcca tggtgaagcc tgcgtattcg tcgggcgcaa gcggcaaatc gtgacgcgcc
     4141 tgaatgacga tctggtagta gagttggacg ctctccggat cgaagcgacg ggccagtgca
     4201 acgatgcgat cgcgaccgga aagatcctcc ggcaacgcat cctcggccat ttgcgccagt
     4261 gccaggcgat gcagcaaggt tcccaactcg atcaatgcgc cgtcaaaggc gatggagcgc
     4321 gattccattt ctccggcaag agcgatcatg cgcgccgcat cgttatcgcc cagcgcttcg
     4381 agcaagtcga acaggtagcc ggtgtccacc gtcccgagca tggcgcgcac cgcttcctcg
     4441 gtcacctggc cgctggaata ggcgatcgcc tgatccagca ggctgagcgc gtcgcgcatg
     4501 ctgccgccgg ccgactgtgc
//
LOCUS       DATGOU010000104        13233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3875179, whole genome shotgun sequence.
ACCESSION   DATGOU010000104 DATGOU010000000
VERSION     DATGOU010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13233
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3875179"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /gene="rimO"
                     /locus_tag="VKZ48_06190"
     CDS             complement(<1..1271)
                     /gene="rimO"
                     /locus_tag="VKZ48_06190"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014896933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HLU77470.1"
                     /translation="MSKVPRVGFVSLGCPKALVDSERILTELRAEGYMISPSYDGADL
                     VIVNTCGFIDEAVEESLDAIGEALTENGKVIVTGCLGAKGTVVQDAHPAVLAVTGPQA
                     TDEVMAVVHKHLPKPHDPYTDLVPPAGIKLTPRHYAYLKISEGCNHHCSFCIIPSMRG
                     DLVSRPIGEVLKEAENLVKSGVKELLVISQDTSAYGVDVKYRTGFWGGRPIRTRMQDL
                     AVALGELDVWVRMHYVYPYPHVDQIVPLMAEGKILPYLDVPFQHASPRILKLMKRPAS
                     GERNLERIHAWRKICPDITLRSTFIVGFPGETEEDFEELLEFLEEAELDRVGCFAYSP
                     VAGAAANELPGAVPEEVKEERRRRFMETQAEISARRLHDKVGKTLRVLIDSVDGKHAI
                     GRSTADAPEIDGLVYVQNGQRLQPGDFIDVR"
     gene            complement(1318..1920)
                     /gene="phaR"
                     /locus_tag="VKZ48_06195"
     CDS             complement(1318..1920)
                     /gene="phaR"
                     /locus_tag="VKZ48_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HLU77471.1"
                     /translation="MVDSVRLIKKYPNRRLYDTRNSCYITLADVKRLVISKEAFQVVD
                     AKSGEELTRSILLQIILEEESGGTPMFTHEVLSEFIRFYGNAMQGFMGEYLQRNIQAV
                     TQFQQSMQPADGAGECCAPDAEAWAKFIDRQTPAMQDLLATYMERSKSVLEEMQRQFQ
                     AQSRSLLESFRLAGMAGMPQQSADQALTEHAAHAPTEEGR"
     gene            complement(2244..3239)
                     /locus_tag="VKZ48_06200"
     CDS             complement(2244..3239)
                     /locus_tag="VKZ48_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HLU77472.1"
                     /translation="MRVLVTGAAGFIGAALSERLLARGDAVVGVDNLDDYYEVALKEA
                     RLARLREHAAFAFERVDIADRAAMQALFERERFDAVVNLAAQAGVRYSVAHPEKYIDA
                     NVVGFGNVLEGCRYGGVKHLVYASTSSVYGLNSKLPFSEDDNVDHPASLYAATKKANE
                     LMAHTYAHLFDLPCTGLRFFTVYGPWGRPDMSPFLFVRAMLAGEPIKVFNHGNMQRDF
                     TYIDDIVEGVVRVLDRPPARGGSADTPATSSAPWRVYNIGNNEPVPLMRYIEVFERCL
                     GLKAKLDLQPLQPGDVVATAADIERLDAAVGFRPATPIEEGVRRFVEWYRAYYRC"
     gene            3295..3498
                     /locus_tag="VKZ48_06205"
     CDS             3295..3498
                     /locus_tag="VKZ48_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77473.1"
                     /translation="MYIVAIAWLYVAILMAATETSVVAGLATFFFYGLAPLLLFWWVV
                     SAPTRKRRKQLQAEAQARAESKD"
     gene            complement(3495..4370)
                     /locus_tag="VKZ48_06210"
     CDS             complement(3495..4370)
                     /locus_tag="VKZ48_06210"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HLU77474.1"
                     /translation="MSTLGYIVAAAVIGGVLSLVCAAIVSFSVRQNWINPLISFAIGA
                     LLGAAFLDILPHALESSGAHTVAGTVLAGILGFFILEKLVLWRHCHLEDCEVHDPPAL
                     GVAPNAHNHAHGHSHGHAHDDRGRSGLMILIGDSFHNFVDGVLIAAAFMQSPALGVVT
                     ATAVIAHEVPQEVGDFLILLHSGYSKARAFALNLLSSLAMLVGALLAYFALQIVQGAV
                     DILLALAAASMIYVAVADLIPGLHKRPELRATAQQVVLILLGIFTVWAVGEISHATMH
                     ADHEPVAIHAPVHEH"
     gene            complement(4375..5112)
                     /gene="pgeF"
                     /locus_tag="VKZ48_06215"
     CDS             complement(4375..5112)
                     /gene="pgeF"
                     /locus_tag="VKZ48_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HLU77475.1"
                     /translation="MNDPHPDWIVPDWPAPAAVKALITTRAGGVSHGAYASFNPSPRV
                     GDDPAAVERNLEILRSVLPADPVWLHQVHGVDVADAESAQPQTQADAAVAHARHIVCA
                     VMTADCLPVLLAHRHGRAVGVAHAGWRGMAAGVIEATLAQLRVPVGEVIAYLGPAISQ
                     QAFEVGPEVHAAFTHNDKEAGAAFAPAASGKYYADLYALARRRLHHAGVREVHGGGFC
                     TYTQAERFYSYRRDRITGRMASLIWLH"
     gene            complement(5112..6080)
                     /gene="rluD"
                     /locus_tag="VKZ48_06220"
     CDS             complement(5112..6080)
                     /gene="rluD"
                     /locus_tag="VKZ48_06220"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: protein motif:HMM:NF008385.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HLU77476.1"
                     /translation="MRSIPIGDHSGEISAADYSPPLAADIPSDLAGMRLDQALARLFP
                     QHSRNRLQHWVREGRVTLDDGPADAKRKVYGGERVSVVPEPPPAETAFRPEAIDLPVV
                     YEDETLLVIDKPAGMVVHPGSGNWEGTMLNALLHHAPQLERVPRAGIVHRLDKDTSGL
                     LVVAKTLEAQTELVRQLQARTVKRVYLALVHGHVDMPDTVDAPIGRHPVERVRMAVVA
                     RGKPARTHYQPVETFADCTLLRCRLDTGRTHQIRVHMHSIGHPLVGDPLYGRRQGRGA
                     LAGFKRQALHAEQLGLVHPRTGEPCEWEAPLPADFAALLRALREEA"
     gene            6079..6879
                     /locus_tag="VKZ48_06225"
     CDS             6079..6879
                     /locus_tag="VKZ48_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HLU77477.1"
                     /translation="MKRSVALLLMALLLAGCAGGGSEDETAGWSAQRLYSEARDMMNS
                     GNYVQAIEYFEKLEARYPFGRYAQQAQLEIAYAYHREGEAASAVAACDRFIKLHPNHP
                     NVDYAYYLKGVANFYEDQTILGQMADQDPAERDPRSARESFNAFRELVTRFPESKYAQ
                     DAQLRMNYIVNALASHEVSVARYYIKRGAYLAAANRAQYAVKTYPQAPAREEALYLMI
                     VSYDQLGMADLRDDARRVLQANFPDSPYLEGKAPVRSAPWWKLWKSGI"
     gene            complement(6884..8845)
                     /locus_tag="VKZ48_06230"
     CDS             complement(6884..8845)
                     /locus_tag="VKZ48_06230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HLU77478.1"
                     /translation="MNPDPVRTDLERIFGPEGPLARAIPGYTPRPQQIDMAARVAQAI
                     AEQGCLIAEAGTGTGKTVAYLVPALLHGGKVIVSTGTKTLQDQLFHRDLPAVRDALGL
                     PVTTALLKGRANYVCRHHLKRNLADGRLSTREDVRDLQAIARFARQTHTGDKAQLPNV
                     REDAPAWALATSTRENCLGTKCGDYNDCFVMKARREALEAEVVVVNHHLFFADVMLKD
                     EGMAELLPACNTVILDEAHQLPDVATLFFGTTVSTGQLLELARDVVADSIAEAPDMRE
                     LQDAARRLDRAARDLRLAVPMKENRAPAETLLRETAFRQALDDLAEACRGALNVLKIA
                     EERGEGLKNCAARAAEILEHVDAWRAGDDAALVRWVEAYTQAVALHATPLVVADQFRR
                     QLEGRSRAWIFTSATLSMNGNFDHYRMQMGLDDADAATWESPFDYPANGIIYLPLELP
                     EPNSAEHTEAVVRAALPVLKASGGRAFLLFTTLRAMRLAHSLLAEHFKREGLAFPLLL
                     QGEGTRSELLERFRQLGNAVLVASSSFWEGVDVRGEALSLVVIDKLPFAPPDDPVMQA
                     RLNALRREGGNPFMDYQLPQAAIALKQGAGRLIRDERDRGVLMICDPRLVSKGYGKTL
                     VDSLPAMRRTRSLPVVERFYAAAADAAAA"
     gene            complement(8842..9663)
                     /locus_tag="VKZ48_06235"
     CDS             complement(8842..9663)
                     /locus_tag="VKZ48_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HLU77479.1"
                     /translation="MRRFWIGLLLCLASWSAMADEVEAIPLQHRTADQVIPALRPLLE
                     PGGALSGMQNTLIIRASRRNIEEIKSILATLDAAPRRLLISVRQDASFSGTRRDYDAS
                     GTVRSGDVRVGVGERPRHETGATVRMLDSARARDERNVSQVQALEGSPAYIAAGQSVP
                     VPVESVRHTPYGREVVRSVEYRDINTGFYVVPRVAGERVILEIAPQRDRPGTYGPGSA
                     DTQHISTTISGRLGEWFELGGISDGSAHEDSRLLSSRSTGARGARSVWVKVEELQ"
     gene            9758..10315
                     /locus_tag="VKZ48_06240"
     CDS             9758..10315
                     /locus_tag="VKZ48_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HLU77480.1"
                     /translation="MTGTAAQFPLMRALWIAAFAAVIGGCAGTPVDVDVDAESRTPAA
                     VAPDVITSEVALLALGQLGVPYRYGGASPRTGFDCSGLVQYVYRQATGAQVPRTTDEL
                     AGAGVRVARDDLHPGDLVFYNTLGRRFSHVGIYLGEGKFIHAPSRNGVVRVEDMRISY
                     WRERYTGARRLALPTTQTAGQDCCR"
     gene            complement(10320..10601)
                     /locus_tag="VKZ48_06245"
     CDS             complement(10320..10601)
                     /locus_tag="VKZ48_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HLU77481.1"
                     /translation="MRTKRLAALAASTLLLAGAPVLTHADDDIAIDKLPQAVRAAIDK
                     RFPGAELLNAEREKDDGRVEYEVELRHEGKRYEVDVTEDGRIVDIDRDD"
     gene            complement(10643..10945)
                     /locus_tag="VKZ48_06250"
     CDS             complement(10643..10945)
                     /locus_tag="VKZ48_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HLU77482.1"
                     /translation="MEALVVGADRLGNIPDVLAEFGIAIRKHVSGRDSAHQKRVVGLP
                     SGIGLVILFTDFLGHNVMKSFREAARAQGLPVVCCRRSTCSVKQALGQCGVCPRRH"
     gene            11107..12132
                     /locus_tag="VKZ48_06255"
     CDS             11107..12132
                     /locus_tag="VKZ48_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HLU77483.1"
                     /translation="MRPRATITALVLGLLLPLGASAQEQVLNIYSARHYKTDEALYDG
                     FTKQTGIKINRIEASEDALIERIKNEGARSPADVLITVDAGRLWRVEQQGLFQPVTSP
                     TLEQRIPAHFREPSGLWYGISMRARVIAYDKSRVQPSEIATYEQLADPKFKGRICVRS
                     SNHIYNLSLLAAMIEHVGEQQATAWAEAVKNNLARAPKGGDTDQIRGVAAGECDVAIA
                     NHYYYARLMRSDKAEDKAVVEKVGVVFPNQATTGTHVNISGVGVLKHAPNRDAAIKFL
                     EYLTSEDAQRYFADGNNEWPVVQGVKVDNPALTSFGEIKYDSLNVSALGRNQPTAQKI
                     YDRVGWK"
     gene            complement(12284..12466)
                     /locus_tag="VKZ48_06260"
     CDS             complement(12284..12466)
                     /locus_tag="VKZ48_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77484.1"
                     /translation="MEQGQGDSSSSLPSNSILNLSVSQLEERLATGIEQRVPQYKDFT
                     AEWPGPLDGRLGYSES"
     gene            12648..>13233
                     /locus_tag="VKZ48_06265"
     CDS             12648..>13233
                     /locus_tag="VKZ48_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HLU77485.1"
                     /translation="MNTPRLPHALTVLVLCLALLAGCSREPVVHRVQGQVFGTAVEIT
                     LYGETAERAQALGAQVMEEFNRLHHKYHAWRPSALTDLNDHIARGERYEGDEEMVYLL
                     RSATALAERSGHTFNPAIGRLVRLWGFHSDRPDAQQPPADEIRRLVDANPRMSDLRYD
                     GMTIWSVNKTVMLDFGGWVKGYALDRAAALLRAAG"
BASE COUNT         2356 a         4397 c         4236 g         2244 t
ORIGIN      
        1 atgcgcacgt cgatgaaatc gcccggctgc aggcgctgtc cgttctgtac atacaccagc
       61 ccgtcgattt ccggcgcatc cgcagtggaa cggccgatgg cgtgcttgcc gtccacgctg
      121 tcgatcagca cccgcagcgt cttgccgacc ttgtcgtgca aacgccgcgc gctgatctcg
      181 gcctgcgttt ccatgaaacg ccgccggcgt tcttccttca cttcctccgg gacggcaccc
      241 ggcaactcgt tggcggccgc cccggctacc ggcgagtagg cgaagcagcc gacccgatcg
      301 agctccgctt cttccagaaa ctccagcaac tcttcgaagt cttcctcggt ctcgccgggg
      361 aaaccgacga tgaaagtgct gcgcagcgtg atgtccggac agatcttgcg ccaggcgtgg
      421 atgcgctcca gattgcgctc acccgaggcg ggccgcttca tcagcttgag gatgcgcggg
      481 ctcgcgtgct gaaacggcac gtcgagatag ggaaggattt tgccctccgc catcagcggg
      541 acgatctggt cgacatgcgg atacgggtag acgtaatgca tccgtaccca cacatcgagt
      601 tcgcccagcg caacagccag atcctgcata cgcgtacgga tgggacgtcc gccccagaaa
      661 ccggtgcggt acttcacgtc cacgccgtag gcgctggtgt cctgcgagat gaccagcagt
      721 tccttcacgc cgctcttcac caggttctcc gcttccttca gcacctcgcc gatcggccgg
      781 ctgaccagat cgccgcgcat ggacgggatg atgcagaagc tgcagtggtg gttgcagccc
      841 tcggaaatct tcagataggc gtaatgccgg ggcgtgagct tgatgccggc gggcggcacg
      901 aggtcggtgt acggatcgtg cggcttgggc aggtgcttgt gcaccacggc catcacttca
      961 tcggtcgcct gcgggccggt gaccgccagc acggcaggat gcgcatcctg caccacggtg
     1021 cccttggcgc cgagacagcc ggtgacgatg accttgccgt tttcggtcag cgcctcgcca
     1081 atggcgtcga gcgactcctc gaccgcctcg tcgatgaagc cgcaggtatt gacgatgacc
     1141 agatcggcgc cgtcatagga cggcgaaatc atataaccct cggcgcgcag ctccgtgagg
     1201 atgcgctccg aatcgacgag cgccttggga cagcctagtg agacgaaacc tactcggggt
     1261 actttgctca ttgaatcggc gtggaagtcg cgcgcctgag cggcgcgccg gatgtgctca
     1321 gcgtccctct tcggtcggag cgtgcgccgc atgttcggtc aacgcctgat cggcggactg
     1381 ctgcggcatc cccgccatgc cggcgaggcg gaagctttcg agcagcgagc ggctttgcgc
     1441 ctggaactgc cgctgcattt cctccagcac gctcttgctg cgttccatgt aggtggccag
     1501 cagatcctgc atcgcgggcg tctgacggtc gatgaatttc gcccacgcct ccgcgtcggg
     1561 tgcgcagcat tcgcccgcgc cgtccgcagg ctgcatgctc tgctgaaact gcgtgacggc
     1621 ctggatgttc cgttgcagat attcgcccat gaagccctgc atggcgttgc cgtagaaacg
     1681 gatgaattcg ctcaagacct catgggtgaa catcggcgtg ccgcccgatt cctcttccag
     1741 gatgatctgc agcaggatgg agcgggtcag ttcctcgcca ctcttggcgt ccaccacctg
     1801 gaacgcctcc ttggagatca ccagccgctt cacgtctgcc agcgtgatgt agcaggagtt
     1861 gcgcgtgtcg tacaaacgcc ggttgggata tttcttgatg agtcgtactg aatcaaccat
     1921 ggttactgct ccatcggacg acaggatgac caatagaacg aggctctacg ccctggcggc
     1981 gaatggagcc aactcgtcgg aagcggtcat cgaaagggga gtgacgcagg ccagcggccc
     2041 tgaaagaggc cgcgtcgcac ctcatggaaa ttcttcgtcg aagccgcgcc caggcgcgcc
     2101 accggcgctg ccggagggac tgaaactgca gaggaaacgc tgatttgatg cacgaaaaac
     2161 cacagaaagc ggcaagggcc gcaaaaagtg cgggctagta ttgcaccgca acaacgacag
     2221 cgtcaaccgc gtcgcccccg cggtcagcag cggtaatagg cccgatacca ctccacgaag
     2281 cggcgcacgc cctcctcgat cggcgtggcc ggccggaagc cgacggcggc atccagccgc
     2341 tcgatatcgg ccgctgtcgc caccacgtca cccggctgca ggggctgcag gtccagcttg
     2401 gccttcaggc caaggcagcg ctcgaatact tcgatatagc gcatcagcgg caccggctcg
     2461 ttgttgccga tgttgtaaac gcgccacggc gcgctgctgg ttgccggcgt atcggccgaa
     2521 ccgccgcgcg ccggcggacg atccagcacg cggaccacgc cttcgacgat gtcgtcgata
     2581 taggtgaagt cgcgctgcat gttgccgtga ttgaacacct tgatcggctc gccggcgagc
     2641 atggcgcgca cgaacaggaa gggcgacata tcgggccgtc cccaaggtcc gtacacggtg
     2701 aagaagcgca ggcccgtgca cggcagatcg aacaggtgcg cataagtgtg cgccatcagt
     2761 tcgttcgcct tcttggtcgc cgcataaagc gaggcgggat gatcgacgtt gtcgtcttcc
     2821 gaaaagggca gcttgctgtt caggccgtac acggaactgg tcgaggcata gaccagatgc
     2881 ttgacgccgc cgtagcggca accttccagc acattgccga agccgaccac gttcgcgtcg
     2941 atgtacttct ccggatgcgc gacggagtag cgcacgcccg cctgcgcggc gagattgacg
     3001 accgcatcga agcgctcgcg ttcgaacagc gcctgcatgg cggcgcgatc ggcgatgtcc
     3061 acacgctcga acgcgaatgc cgcgtgttcg cgcaggcgcg ccagccgcgc ttccttcagg
     3121 gcgacttcgt agtaatcgtc gagattgtcc acgccgacga ccgcatcgcc gcgcgcaagc
     3181 agccgttcgc tcaacgccgc gccgatgaag cctgccgccc ccgtcaccaa tacccgcata
     3241 acgcttccct ggtctgtcct acaatcggcg gcgtattcac cgccgctccc attcatgtac
     3301 atcgtcgcca tcgcctggct ttacgtcgcc atcctgatgg ccgccaccga aacgagcgtc
     3361 gtcgccggcc tggccacttt cttcttctac ggcctcgcgc cgctgctgct gttctggtgg
     3421 gtggtcagcg caccgacgcg caaacgccgc aaacaactgc aggccgaagc ccaagcgcgc
     3481 gccgaaagca aagactagtg ctcgtgcacc ggcgcgtgta tcgcgaccgg ttcgtgatcc
     3541 gcatgcatgg tcgcatgact gatctcgccc accgcccaca cggtgaagat gccaagcagg
     3601 atcagcacca cctgttgcgc cgtcgcccgc agctcgggcc gcttgtgcag acccgggatg
     3661 aggtcggcaa cggcgacata gatcatgctc gcagccgcca atgccagcag gatatcgaca
     3721 gcgccctgca cgatctgtaa cgcaaagtac gcgagcagcg ccccgaccag catcgccaga
     3781 ctcgacagca ggttcagcgc gaaagcgcgc gccttgctat agcccgagtg cagcaggatg
     3841 aggaagtcgc ccacttcctg cggcacttcg tgcgcgatga cggccgtggc ggtgacgacg
     3901 ccgagcgcag ggctttgcat gaaggcggcg gcgatcagca cgccgtcgac gaagttgtgg
     3961 aagctgtcgc cgatgagaat catcaggccg ctgcggccgc gatcatcgtg cgcatgccca
     4021 tgcgaatggc cgtgcgcatg attgtgcgcg ttcggcgcaa cgcccagcgc gggcgggtca
     4081 tgcacttcgc agtcttccag gtgacagtgt cgccacagca ccagcttctc cagaatgaag
     4141 aagccgagga tgccggccag cacggtgccc gccacggtgt gcgcaccgct gctttccagc
     4201 gcatgcggca ggatgtcgag aaaggccgcg ccgagcagcg cgccgatggc gaagctgatc
     4261 agcgggttga tccagttctg acgcacgctg aacgagacga tggcggcaca caccagcgac
     4321 agcacgccgc cgatcaccgc cgcagcgacg atataaccaa gggtactcat ttattcaatg
     4381 cagccagatg aggctcgcca tgcgaccggt gatccggtcg cggcggtagg aatagaagcg
     4441 ttcggcctgc gtgtaggtgc agaacccgcc gccgtgcact tcgcgcacgc ctgcgtggtg
     4501 gagacgccgg cgtgccagcg cataaagatc ggcatagtac ttccctgatg cggccggcgc
     4561 gaaggcagcg cccgcctcct tatcgttatg ggtaaaggcc gcatgcacct ccggcccgac
     4621 ttcgaaagcc tgttgcgaaa tggcgggacc caaataggcg atgacttcgc cgacgggcac
     4681 gcgcagttgc gccagcgtcg cctcgatcac gccggccgcc atgccacgcc acccggcatg
     4741 cgccacgccg accgcgcggc cgtgccggtg cgccagcagc acgggcaggc agtcggcggt
     4801 catcacggcg cagacgatat gccgcgcgtg cgccaccgcg gcatccgcct gagtctgtgg
     4861 ttgcgccgac tcggcgtccg ccacatcgac gccgtgcacc tgatgcagcc acaccggatc
     4921 ggccggcagc acgctgcgca gaatttccag attgcgttcc actgcggccg gatcatcgcc
     4981 gacgcggggg ctgggattga aggacgcgta ggcgccgtgg ctcacgcccc cggcgcgggt
     5041 ggtgatgagc gccttcaccg cagccggtgc gggccagtcg ggcacgatcc agtcgggatg
     5101 cgggtcgttc atcaggcttc ttcgcgcagg gcgcgcagca gcgcggcgaa gtccgcaggc
     5161 agcggcgctt cccactcaca cggctcgccg gtgcgcggat gcaccagccc cagttgttcg
     5221 gcatgcagcg cctgccgctt gaatccggcc agcgcaccac gcccctgccg gcggccgtag
     5281 agcggatcgc ccaccagcgg gtgcccgatg gaatgcatgt ggacgcggat ctggtgcgtg
     5341 cgcccggtat cgagccggca gcgcagcagc gtgcagtcgg cgaaggtttc gaccggttgg
     5401 taatgcgtgc gcgccggttt gccgcgcgcc acgacggcca tgcgcacccg ctcgaccgga
     5461 tgtcggccga tgggcgcgtc cacggtatct ggcatatcga cgtgcccgtg caccaatgcg
     5521 agatagacgc gcttcacggt gcgcgcctgc aactggcgca cgagttcggt ctgcgcttcg
     5581 agggtcttcg ccaccaccag cagtccgctg gtgtccttgt ccagccgatg gacgatgccc
     5641 gcgcgcggca cccgctccag ttgcggcgca tggtgcagca gcgcattgag catggtgccc
     5701 tcccagttgc cgctgcccgg atgcaccacc atgccggccg gcttgtcgat gacgagcagg
     5761 gtctcgtcct cgtacaccac gggcaggtcg atggcttcgg gacggaacgc cgtctcggca
     5821 ggcggcggtt cggggacgac ggaaacgcgc tcgccgccat agaccttgcg cttggcatcg
     5881 gccggcccgt cgtccagcgt cacccgccct tcgcgcaccc agtgctgcag gcggttgcgg
     5941 gaatgctggg ggaacagtcg cgccagggcc tgatcgaggc gcattccggc caaatcggac
     6001 ggaatgtcgg cagccagcgg cgggctataa tccgccgcgg aaatctctcc gctgtgatca
     6061 ccgatcggga tggaacgcat gaagcgtagt gtagcgttat tgcttatggc cctgttgctc
     6121 gcgggttgtg ccgggggcgg cagcgaagac gaaacggccg ggtggtcggc gcagcgcctg
     6181 tacagcgagg cgcgcgacat gatgaacagc ggcaactacg tccaggcgat cgagtatttc
     6241 gagaagctgg aggcgcgcta ccccttcggc cggtacgccc agcaggcgca actggaaatc
     6301 gcctacgctt accatcgcga aggcgaagcg gcctcggccg tcgccgcctg cgaccgcttc
     6361 atcaagctgc accccaacca cccgaacgtc gattacgcct actacctgaa gggcgtggcg
     6421 aacttctacg aggaccagac catcctcggg cagatggccg accaggaccc ggccgagcgc
     6481 gacccgcgtt cggcgcgcga gtccttcaac gccttccgcg aactggtgac gcgctttccc
     6541 gagagcaagt acgcccagga tgcgcaactg cgcatgaact acatcgtcaa cgcgctcgcc
     6601 tcgcatgaag tctcggtggc gcgctactac atcaagcgtg gcgcctatct ggccgccgcc
     6661 aatcgtgccc agtatgcggt gaagacctat ccgcaggccc cggcgcgcga ggaagcgctg
     6721 tacctcatga tcgtatccta cgatcaactc ggcatggccg acctgcgcga cgacgcccgc
     6781 cgcgtgctgc aagccaactt ccccgacagc ccctacctcg agggcaaggc gccggtgcgc
     6841 agcgcgccgt ggtggaagct gtggaagtcc ggtatctgag cgatcaggcc gccgccgcgt
     6901 ccgccgcggc ggcatagaag cgttccacca ccggcagact gcgcgtacgc cgcatggcgg
     6961 gcagactgtc caccagcgtc ttgccgtagc ccttgcttac cagccgcggg tcgcaaatca
     7021 tcagcacgcc gcgatcgcgt tcgtcgcgga tcaatcgtcc ggcgccctgc ttcagggcga
     7081 tcgccgcctg cggcaactga tagtccatga acgggttgcc gccctcgcgg cgcaaggcgt
     7141 tcagccgcgc ctgcatcacc ggatcgtccg gcggcgcaaa aggcagcttg tcgatcacca
     7201 ccagggacag cgcctcgccg cgcacgtcca ccccctccca gaaactggaa ctggcgacca
     7261 gcaccgcatt gcccaactga cggaagcgct ccaatagttc gctgcgcgtg ccctcaccct
     7321 gcagcaggag cggaaaggcg aggccttcgc gcttgaagtg ttcggcgagc aggctgtgcg
     7381 caagccgcat ggcgcgcagc gtggtgaaca gcagaaaggc acggccgccg ctggccttga
     7441 gcaccggcag cgcggcgcgc accaccgcct cggtgtgttc cgcactgttc ggctccggca
     7501 attcgagcgg cagatagatg atgccgttgg ccggatagtc gaaggggctt tcccaggtgg
     7561 cggcatccgc atcgtccagc cccatctgca tccggtaatg atcgaagttg ccgttcatcg
     7621 acagcgtggc cgaggtgaaa atccacgcgc ggctacggcc ttccagttga cggcggaact
     7681 gatcggccac cacgagtggc gtagcatgca gcgcgaccgc ctgcgtataa gcctccaccc
     7741 agcgcaccag ggcagcgtcg tcgccggcgc gccatgcgtc cacgtgttcg aggatttcgg
     7801 ccgcgcgggc ggcgcagttc ttcaatccct cgccgcgctc ctcggcgatc ttgaggacgt
     7861 tgagcgcgcc gcgacaggct tcggccagat cgtccagcgc ctgccggaac gcggtttcgc
     7921 gcagcagcgt ttccgccggc gcacggtttt ccttcatcgg caccgcaaga cgcagatcgc
     7981 gcgccgcacg atcgaggcgc cgcgcggcat cctgcagttc gcgcatgtcc ggcgcttcgg
     8041 caatgctgtc ggccaccacg tcacgcgcca gttcgagcaa ctggcccgtg gagacggtgg
     8101 tgccgaagaa cagcgtcgcg acatcgggga gttgatgcgc ctcgtccaga atcacggtat
     8161 tgcaggcagg cagcaactcg gccataccct cgtccttgag catgacgtcg gcgaagaaca
     8221 aatggtgatt gaccaccacc acttcggcct ccagcgcttc gcgccgcgcc ttcatgacga
     8281 agcagtcgtt gtagtcgccg catttggtgc ccaggcagtt ctcgcgcgtc gaagtcgcca
     8341 gcgcccaggc cggcgcgtcc tcgcgcacat tcggcagttg cgccttgtcg ccggtgtgcg
     8401 tttgccgggc gaagcgggcg atggcctgca gatcgcgcac gtcctcgcgc gtggaaagcc
     8461 ggccgtcggc caggttgcgc ttcagatgat gacggcagac atagttcgcg cgtcctttca
     8521 gcagcgccgt ggtgacgggc aggccgagcg catcgcgcac ggccggcaga tcgcgatgga
     8581 acaactggtc ctgcagcgtc ttggtgccag tggagacaat gaccttgccg ccgtgcaaca
     8641 gcgccggcac cagataggcg acggtcttgc ctgtgccggt accggcctcg gcgatcagac
     8701 agccttgctc cgcaatggct tgcgcgacgc gcgcggccat gtcgatctgc tgcggacggg
     8761 gggtatagcc ggggatggcg cgggccagcg gcccttccgg accgaagatg cgttcgaggt
     8821 ccgtgcggac cggatcagga ttcactgcag ttcttcgact ttcacccaca cgctgcgcgc
     8881 gccgcgcgcg ccggtggaac gactggaaag aaggcggctg tcttcgtgcg cgctgccgtc
     8941 gctgatgccg cccagttcga accactcgcc cagccggccg ctgatagtgg tgctgatgtg
     9001 ctgcgtgtcc gcgctgccgg gcccgtaggt gccgggccgg tcgcgctgcg gcgcgatttc
     9061 gagaatgacg cgctcgccgg ctacacgcgg cacgacgtag aagccggtgt tgatgtcgcg
     9121 gtactcgacg ctgcgcacca cttcgcggcc gtaaggcgtg tgccgcacgc tttcgacggg
     9181 caccggcacc gactgacccg cggcgatgta ggcgggactg ccttccagcg cctggacctg
     9241 gctgacgttg cgttcgtcgc gagcgcgcgc cgagtcgagc atgcgcacgg tggcgccggt
     9301 ttcgtggcgc ggccgctcgc ccaccccgac gcgcacgtcg ccgctgcgga ccgttcccga
     9361 tgcgtcgtag tcgcgacgcg tgccgctgaa ggaggcgtcc tgccgcacgg aaatgagcag
     9421 ccgccgcggc gctgcgtcga gcgtcgccaa tatgctcttg atctcttcga tattgcgccg
     9481 gctggcgcgg atgatgagcg tgttctgcat gccggacagt gcaccgcccg gttcgagcaa
     9541 tggccgcagg gcgggaatca cctgatcggc ggtgcgatgc tgcaacggga tcgcttcgac
     9601 ctcgtcggcc attgcggacc acgatgcaag acacagcaac aacccgatcc agaaccgacg
     9661 catgtgttat tcctcgaccc cctgcggcga aagtgctggc cgccgcggcg caggcttcct
     9721 acactacccg gcgatgggga ccaaggcaat gttgagaatg acagggaccg cagcgcagtt
     9781 cccgctcatg cgcgcgttgt ggatcgcggc gttcgcagcc gtgatcggcg gctgcgcggg
     9841 cacacccgtg gacgtggacg tcgatgcgga atcgcgcacc cctgcggcgg tggcgccgga
     9901 tgtcatcacc agcgaagtgg cgctgctcgc actgggtcag ctgggcgtgc cctatcgcta
     9961 cggcggcgcg tcgccgcgga cgggcttcga ttgttcgggg ttggtgcaat acgtctatcg
    10021 tcaggcgacc ggcgcccagg tgccgcgtac gactgacgaa ctcgcgggcg ccggcgtgcg
    10081 cgtggcgcgc gacgatctgc accccggcga tctggtgttc tacaacaccc tggggcgccg
    10141 cttttcacac gtcgggattt atctgggcga agggaaattc atccacgcgc cgagccgcaa
    10201 cggcgtcgtg cgcgtggagg atatgcgcat ctcctactgg cgcgagcgct acaccggcgc
    10261 gcgtcgcctc gcactgccca cgacgcagac ggccgggcag gactgctgcc gctaaaggat
    10321 caatcgtcgc ggtcgatgtc gacgatgcgg ccgtcctcgg tcacgtcgac ttcgtagcgc
    10381 ttgccttcgt ggcgcagttc cacttcgtat tccacgcgcc catcgtcctt ctcacgctcg
    10441 gcgttgagca attcggcgcc ggggaagcgc ttgtcgatgg cggcgcgaac cgcttgcggc
    10501 agcttgtcga tggcgatgtc gtcatcggca tgcgtcagca caggtgcgcc ggccagcaat
    10561 agcgtcgatg ctgccagtgc agcgagccgt ttggttctca tatccgttcc ttcgcttgcg
    10621 ggacggcgga tgagaggcaa gcctagtgcc gtcgggggca cacaccgcat tgccccaagg
    10681 cctgcttcac actgcaggtg gagcggcggc agcacaccac cggcaatccc tgcgcacgcg
    10741 cagcttcgcg aaagctcttc atgacgttgt ggccgagaaa gtcggtgaac agaatcacca
    10801 gaccgatgcc gctgggaagg ccgaccacgc gcttctgatg ggcggaatcg cgcccgctga
    10861 catgctttct gatggcaatg ccgaactcgg ccagtacgtc gggaatgttg ccgagccggt
    10921 ccgcgccgac gacgagtgcc tccacttgcc gctcctgggt ttgctgtaat gtcgagaatg
    10981 ataatggttc tcattactgt gtcaagcaca atgggaatcg ttctcgttat ccttggcaag
    11041 cccccgggcg cgctgtagca tccgccccga cccctcatta cgaagaacag gagaaacgtc
    11101 atggccatgc gcccacgcgc aacgatcacc gcgctggtcc tgggcctgct gctgcctctg
    11161 ggagcaagcg cacaggaaca ggtactgaac atctattccg cccgtcacta caagaccgac
    11221 gaggcgctgt acgacggctt caccaagcag accggcatca agatcaaccg catcgaggcg
    11281 agcgaggacg cgctgatcga gcgcatcaag aacgaaggtg cgcgcagccc ggcggacgtg
    11341 ctgatcaccg tggacgcggg tcggctctgg cgcgtggagc agcaaggact gtttcaaccc
    11401 gtgacctccc ccaccctgga gcagcgcatt ccggctcact tccgcgagcc gagcggcctg
    11461 tggtacggca tctccatgcg cgcccgcgtc atcgcgtacg acaagtcgcg cgtccagccc
    11521 agcgagatcg cgacctatga acaactggcc gatccgaaat tcaaggggcg catctgcgtg
    11581 cgttcctcca accacatcta caacctctcg ctgctcgccg cgatgatcga acacgtgggc
    11641 gaacaacaag cgacggcttg ggccgaggcg gtgaagaaca acctcgcccg cgcgccaaaa
    11701 ggcggcgaca ccgaccagat ccgcggcgtg gccgccggcg aatgcgatgt ggcgattgcc
    11761 aaccattatt actacgcgcg cctgatgcgc tcggacaaag ccgaggacaa ggcggtggtc
    11821 gagaaagtcg gcgtggtctt ccccaatcag gcgacaaccg gcacgcacgt caacatttcg
    11881 ggcgtgggcg tgctcaagca cgcccccaac cgcgacgcgg cgatcaagtt cctcgaatat
    11941 ctgacgagcg aggatgcgca gcgctacttt gccgacggca acaacgagtg gccggtcgtg
    12001 cagggcgtga aggtggacaa tcccgccctc accagtttcg gggagatcaa gtacgactcg
    12061 ctaaacgtct cggcgctggg acgcaatcag cccaccgcac aaaagatcta cgaccgcgtc
    12121 ggctggaagt aagcctcgtc cgcacggcaa cccgaagccc cgccttgcgc ggggcttctt
    12181 ttttgcgcgc cggcggcagg cttcaggacg aaaaaaaggc cgcgaatcgg aatcgatccg
    12241 cggcctttac tccctgagct tgctgctcct gtaaccgggg ggcttagctc tcggaataac
    12301 cgaggcgccc gtcgagcggc ccgggccact cggctgtgaa atctttgtac tgagggaccc
    12361 gctgttcgat gccggtcgcc agacgctcct cgagttgaga aacggaaagg ttgagaatgc
    12421 tgttgctggg taatgaacta ctactgtcgc cttgaccttg ttccacgatt cgctccaaat
    12481 gaatgcgacg ccattatgcg cttacccgtc aaacgcacct ataaagattt cacactgcaa
    12541 acctgccggc gttgtacgcg cgcgacatat tccgcacgcg ccccgcgacg gatgcccgcc
    12601 gcaagcgcct gcgcaggcat ccggccggct tccttggaat gagaccgatg aatacccctc
    12661 gccttccgca tgcgctcacc gttctggtcc tgtgcctcgc actcctcgcc ggctgcagtc
    12721 gggagccggt cgttcatcgg gtgcaaggtc aggtgttcgg cactgcggtg gaaatcactc
    12781 tgtacggcga gacagccgaa cgggcgcagg cgctcggcgc gcaggtcatg gaggagttca
    12841 accgcctgca ccacaagtac cacgcctggc ggcccagcgc gctcaccgat ctgaacgacc
    12901 atatcgcgcg cggcgagcgc tacgagggcg acgaggaaat ggtctatctg ctccgctcgg
    12961 cgacggcgct ggcggagcgg tcgggccaca ccttcaatcc ggcgatcggt cgcctcgtcc
    13021 ggctatgggg ctttcatagc gaccggcccg acgcgcaaca gccgcccgcc gatgaaatcc
    13081 gccgactggt cgacgcaaat ccgcggatga gcgacctgcg ctacgacggc atgaccatct
    13141 ggagcgtcaa caagaccgtc atgctggact tcggcggttg ggtgaaaggc tacgcactcg
    13201 atcgcgcagc cgccctgctg cgcgcggccg gcg
//
LOCUS       DATGOU010000105         2374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_5638889, whole genome shotgun sequence.
ACCESSION   DATGOU010000105 DATGOU010000000
VERSION     DATGOU010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5638889"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1157)
                     /gene="hisC"
                     /locus_tag="VKZ48_06270"
     CDS             complement(24..1157)
                     /gene="hisC"
                     /locus_tag="VKZ48_06270"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006388259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HLU77486.1"
                     /translation="MSDTPFDPCEHAPAHIRSIAPYQPGKPITELARELGLEPDSIVK
                     LASNENPLGMSPHVLAAIQMALPELHRYPDNFALTKRIATRLDRPMESIVLGNGSNDV
                     LEMVAAVFLGPGRSSVFSQHAFAVYPLATQARGARSIVVPADGYAHDLNAMLHAIADD
                     TRVIFIANPNNPTGTMLSPDELQHFLSKVPPHVIVVLDEAYNEYLPDAANYDSRRWLD
                     DHPNLVITRTFSKAYGLAGLRVGYGLCDPRVADLLNRVRQPFNVNNLALVAAEAALED
                     QEFVRESHLLNRAGMKQLEHGFEALDLRWIPSYGNFVSVEVPRADGVSQGGAVYQKLL
                     RAGVIVRPVAGYGMPDHLRVTVGLAEENRRFLEALAVALGREL"
     gene            complement(1154..2227)
                     /gene="pheA"
                     /locus_tag="VKZ48_06275"
     CDS             complement(1154..2227)
                     /gene="pheA"
                     /locus_tag="VKZ48_06275"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HLU77487.1"
                     /translation="MSEDEIRKHREAIDRYDDELLKLISERSQHVLAVGRIKGADAQK
                     QRPDREAQILRRMVANNPGPLSAEAVMAIWRELISQSMALEQQLVVSYLGPAGTFSHV
                     AAKTHFGTAPELVPCGSIDEVFRLAETGTADFAVVPVENSTEGAVGRTLDLLLQTPLT
                     ICAEIVLRVRQNLLRKTNSLEGVARVYSHSQSLAQCQNWLARHLPGAARIPVASNAEA
                     ARLAASEPDACAIGPALAAEHYELQIVAPDIEDEAHNATRFLVLGKVACGPTGDDLTS
                     IVMSAPNKPGAVHGLLSPIAAHGISMTRIESRPSRLARWEYMFYVDLLGHADEPEVAQ
                     VLKELKELAPFLKVLGAYPRAPR"
BASE COUNT          408 a          746 c          782 g          438 t
ORIGIN      
        1 gcggctttgc cgtactgcgc caattacagc tcccggccca gcgccacagc gagggcttcg
       61 aggaaacggc ggttctcttc ggccaacccc accgtgacgc gcaaatgatc cggcatgccg
      121 tagccggcga cgggccgcac gatgacgcct gcgcgcagca gcttctgata gacggcgccg
      181 ccctggctta cgccgtccgc gcgcggcact tccacgctga cgaaattgcc gtaggacgga
      241 atccagcgca gatcgagcgc ttcgaacccg tgctccagct gcttcatgcc agcgcggttg
      301 agcagatggc tttcgcgcac gaactcctgg tcctccagcg ccgcttccgc cgcgacgagt
      361 gcgaggttgt tgacgttgaa gggctggcgc acgcgattga gcagatcggc cacgcgcgga
      421 tcgcacaggc cgtagcccac gcgcaggcct gcgagcccgt acgctttgga gaaagtccgc
      481 gtaatgacga ggttgggatg atcgtcgagc cagcgccggc tgtcgtagtt cgccgcatcc
      541 ggcagatatt cgttgtaggc ctcgtcgagc acgacgatca catgcggcgg cactttactc
      601 agaaagtgct gcaactcgtc gggcgacagc atcgtcccgg tcggattgtt cggattggcg
      661 atgaagatga cgcgcgtgtc gtccgcgatg gcatgcagca tcgcgttgag atcgtgcgca
      721 taaccgtcgg ccggcacgac gatgctgcgc gcgccgcgtg cctgggtcgc cagcggatag
      781 acggcaaagg cgtgctgcga gaataccgac gagcggcccg gccccagaaa aacggcggcg
      841 accatttcca gcacatcgtt cgaaccgttg ccgagcacga tgctttccat cggccggtcg
      901 agccgggtgg caatgcgctt cgtcagcgca aaattgtccg gatagcggtg cagttccggc
      961 agcgccatct ggatcgccgc aagcacatgc ggcgacatgc cgagcgggtt ctcgttggag
     1021 gccagcttga cgatggaatc gggctcgagc ccgagttcgc gtgccagttc ggtgatcggc
     1081 ttgcccggct gataaggggc aatactgcgg atatgcgcgg gcgcgtgctc gcagggatcg
     1141 aaaggtgtat cgctcatcgg ggtgcgcgcg ggtaggcgcc cagcactttg agaaagggcg
     1201 cgagttcctt gagttccttc aggacctggg cgacttcggg ttcgtctgca tgaccgagca
     1261 gatcgacata gaacatgtat tcccagcgcg ccaggcgcga gggccgcgat tcgatccgcg
     1321 tcatgctgat gccgtgcgcg gcgatcgggc tgagcagtcc gtgcactgcg ccgggtttgt
     1381 tgggcgcgga catcacgata gacgtgagat cgtcgccggt cgggccgcat gccactttgc
     1441 ccagcaccag gaagcgcgtg gcgttgtgcg cctcgtcctc gatatcgggt gccacgatct
     1501 gcagttcgta gtgttccgcc gccagcgcgg gaccgatggc acaggcatcg ggttcgctgg
     1561 cagccagacg cgccgcttcg gcgttgctcg ccacgggaat gcgcgccgca ccgggcagat
     1621 ggcgtgcgag ccagttctgg cattgcgcca acgactgcga atgcgagtag acgcgggcga
     1681 caccttccag cgagttggtc ttgcgcagca gattctggcg cacacgcagc acgatttccg
     1741 cacagatagt caagggcgtc tgcagcagca gatccagcgt acggccgacg gcgccctcgg
     1801 tggaattctc caccggcacc acggcgaaat ccgccgtgcc ggtctcggcc agacggaata
     1861 cctcgtcgat ggagccgcag ggcacgagtt ccggcgccgt gccgaaatgc gtcttggccg
     1921 ccacgtggct gaaggtgccg gccggaccca gataggacac gaccagttgt tgttccagcg
     1981 ccatgctctg gctgatgagc tcgcgccaga tcgccatcac cgcttcggcc gacagcggac
     2041 cgggattgtt cgccaccatg cgacgcagaa tctgcgcttc gcgatccggg cgctgcttct
     2101 gcgcgtcggc gcccttgatg cggccgacgg ccagcacgtg ttgggatcgc tcgctgatca
     2161 gtttcagcag ctcgtcgtcg tatcggtcga tggcctcccg atgcttgcgg atttcgtctt
     2221 cgctcattgt tcgaccggta gatagcgcac ggccagtacg ccgtcgatgc gtccgatgct
     2281 ctcgacgacc gacgcggcca ccgggctatc gacatcgacc agcgtgtacg ccatgtcgcc
     2341 tttcgatttg ttgaccatat tgtgaatgtt cagc
//
LOCUS       DATGOU010000106         2514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_757854, whole genome shotgun sequence.
ACCESSION   DATGOU010000106 DATGOU010000000
VERSION     DATGOU010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757854"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="VKZ48_06280"
     CDS             complement(<1..1125)
                     /locus_tag="VKZ48_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HLU77488.1"
                     /translation="MSESKTEQPWWRGAVVYQIFPDSFADSNGDGRGDAQGLLQRLEY
                     LQWLGVDALWLSPMYPSPMADGGYDISDHKGVHPHFGTLEDMDRLIAEAHRLGLRVLL
                     DYVPNHTSDEHPWFVESRSSRNNAKRDWYIWRDSTNNWRAGINCKEAWTWDEHTRQHY
                     LHLFLPQQPDLDWRNPAVVDAMHDVLRFWIERGIDGFRMDAVHCIGKDMSFADDPRCD
                     EGDPISLFNHQPSTHDVLRGIRRFVDALERDTLLVGEVNIRDTRAVVEYYGDDDEVHM
                     SFNFLPIILPWEAKVFRDCICEIESIYTPAKAWPTWVLSNHDRVRHRTRYGGSMARAR
                     AAAVMLLTLRGTPFMFQGEELGLEEAKIHPDDWEDPSGRDG"
     gene            1261..1584
                     /locus_tag="VKZ48_06285"
     CDS             1261..1584
                     /locus_tag="VKZ48_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77489.1"
                     /translation="MQPSEVQQRFQRVEQCIDDAYQACQQSQSAPQQLKQSIQQLDQS
                     ADQARQYIQGQDQGKIKQCIYELEELSDRALEACRQAGNVDPQLKQAVQQTHSELSSL
                     KHQMG"
     gene            1709..>2514
                     /locus_tag="VKZ48_06290"
     CDS             1709..>2514
                     /locus_tag="VKZ48_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HLU77490.1"
                     /translation="MKAVGYSKSLPIEHEHSLVDFDCPAPKASGHDLLVEVKAVSVNP
                     VDTKVRMRAEPPEGEYKILGWDAAGIVREVGEAVTLFKPGDRVWYAGALDRSGSNAEF
                     QLVDERIVGPMPGSLDFAQAAALPLTSLTAWELMFDRLQVPRGEEERDECLLIIGAAG
                     GVGSIMLQLARRLTGLTIIGTASRPQTQEWARRLGAHHVIDHRNPIAEELKRIGIPQV
                     EYIASLTHTDTHFEQAVEALAPQGRFGLIDDPQPIDVRLLKRKSASLHWEL"
BASE COUNT          522 a          826 c          751 g          415 t
ORIGIN      
        1 gccgtcgcgg ccggaaggat cttcccagtc gtcgggatga atcttcgctt cctccagccc
       61 cagttcctcg ccctgaaaca tgaagggcgt accgcgcagc gttagcagca tcaccgcagc
      121 cgcccgtgcg cgcgccatcg agccgccgta gcgcgtgcga tggcggacgc gatcatggtt
      181 cgacagcacc cacgtgggcc aagccttggc cggcgtgtag atcgattcga tctcgcagat
      241 gcagtcgcgg aaaaccttcg cttcccacgg caggatgatc ggcaggaaat tgaacgacat
      301 gtgtacttcg tcgtcgtcgc cgtaatactc caccacggcg cgcgtatcgc ggatgttcac
      361 ctcgccgacc agcagcgtgt cgcgttccag ggcatcgacg aagcggcgga tgccgcgcag
      421 cacgtcgtgc gtggagggct ggtgattgaa cagcgagatc gggtcgcctt cgtcgcaccg
      481 cggatcgtcg gcgaaactca tgtccttgcc gatgcaatgc accgcgtcca tgcggaagcc
      541 gtcgataccg cgttctatcc agaagcgcag cacgtcgtgc atcgcatcga caacggcggg
      601 gttgcgccag tcgaggtccg gctgctgcgg caggaacaga tgcagatagt gctgccgcgt
      661 gtgctcgtcc caggtccacg cttccttgca attgatgccc gcgcgccagt tgttggtcga
      721 gtcgcgccag atgtaccagt cgcgtttcgc gttatttcgt gacgaacgcg attcgacaaa
      781 ccagggatgc tcatcggaag tgtgattggg aacgtagtcg agcagcacgc gcaagccgag
      841 gcgatgcgct tcggcgatga gccggtccat gtcttccagc gtgccgaaat gaggatgcac
      901 gcctttgtgg tccgaaatgt cgtaaccgcc gtccgccatg ggcgacggat acatgggcga
      961 cagccacagc gcgtcgacgc ccagccattg caaatactcc aatcgctgca gcaggccctg
     1021 cgcgtcgccg cgcccgtctc cgttcgaatc cgcaaaggaa tcgggaaaga tctgatacac
     1081 gaccgcgccg cgccaccacg gctgttctgt cttgctctcg ctcataacat cgccgctgac
     1141 caggacgacg cgcatgcagc aaacgatatg ccccggacga ggaacaggcc ttgcaaccca
     1201 atcttcgatg cgttctacgg agcgcactgt ccagagcaaa cgcatgcaaa aggagacacc
     1261 atgcaaccca gcgaagtaca acaacgcttt caacgcgtcg aacaatgcat cgacgacgcc
     1321 tatcaggcct gccagcaaag ccagtctgcg ccgcagcaac tgaagcaatc gatccagcag
     1381 ctggaccaaa gcgccgacca ggcgcgccag tacatccaag gccaggacca gggcaagatc
     1441 aagcaatgca tttacgaact ggaagaactg agcgaccgcg cgctggaagc ctgccgccag
     1501 gccggcaatg tcgatccgca actgaagcag gcggtgcagc aaacgcatag cgagctgtcc
     1561 agcctgaagc atcagatggg ttgagcctcg agcccagcca tgaagcaagg gccgcctcgt
     1621 gcggcccttt gtcatggcac ccgtgcggtt gccagcgcgt gcatccgcgc gctaggcttt
     1681 ccgtattgcg ttcgaaccga ggctcagcat gaaagcggtc ggctacagca aatccctgcc
     1741 catcgaacac gagcattcgc tcgtcgattt cgactgcccc gcgcccaagg ccagcggcca
     1801 cgatctgctg gtcgaagtca aagcggtatc ggtcaatccg gtggatacca aagtccgcat
     1861 gcgcgccgaa ccgcccgaag gcgaatacaa gattctcggc tgggacgctg cagggatcgt
     1921 gcgagaggtc ggcgaagccg tcacgctgtt caagccgggc gatcgcgtct ggtatgccgg
     1981 cgcgctcgac cgctccggca gcaacgcgga gtttcaactc gtcgacgagc gcatcgtcgg
     2041 accgatgccg ggaagcctcg acttcgcgca ggccgccgca ctgccgctga ccagcctcac
     2101 cgcctgggaa ctgatgttcg accgcctgca ggtcccacgt ggcgaggaag agcgggatga
     2161 atgcctgctg atcatcggcg ccgccggcgg cgtcggctcg atcatgctgc aactcgcgcg
     2221 gcgcctcacg ggcctcacca tcatcggcac cgcttcacgc ccgcaaacac aggaatgggc
     2281 gcgcaggctc ggtgcacacc acgtgatcga tcaccgcaat ccgatcgccg aagaactgaa
     2341 gcgcatcggc atcccgcagg tcgaatacat cgccagcctc acgcacaccg acacgcactt
     2401 cgagcaggca gtcgaagcgc ttgcgccgca aggccgcttc ggcctcatcg acgatccgca
     2461 gcccatcgac gtccgtctgc tcaagcgcaa gagcgcctca ctgcattggg aact
//
LOCUS       DATGOU010000107         6123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_5645047, whole genome shotgun sequence.
ACCESSION   DATGOU010000107 DATGOU010000000
VERSION     DATGOU010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5645047"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VKZ48_06295"
     CDS             complement(<1..613)
                     /locus_tag="VKZ48_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77491.1"
                     /translation="MAVSVSILLSVWLTYSDNVINGDGILYLKSAEAFADGDWRQGVT
                     IYRWPLYAALIGALHYVSGLDTDIVAYVVNGALYAVVVWTFLSIVKLFGADRRTLWIA
                     AILILALPSLNSYRAFVIRDVGYWAFYLVGLLAFFKFQLTPRLRLALGFGLAMTVATL
                     FRIEGIAFLLFMPLVVLWDAGISHRERLARLAKLNIVLLVNAVV"
     gene            complement(656..1453)
                     /gene="rfaP"
                     /locus_tag="VKZ48_06300"
     CDS             complement(656..1453)
                     /gene="rfaP"
                     /locus_tag="VKZ48_06300"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011703.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HLU77492.1"
                     /translation="MTEFYVAPALKEALGGDVSFDMFMNMPGEVFREIASRRTVRFQA
                     GAKSYFVKAHRGVGWVEILKNLCTLRLPVLGAMNEWRAIRRLEAAGIRAPMIAAYGRR
                     GRNPAAMDSFLVTEDVGQAVSLEDHCGRWPENPPGFSDKQALLREVALISRALHQSGV
                     NHRDYYLCHFLCPLSGADRRLVLIDLHRAQVRRKVPRRWLIKDLGGLYFSAMDIGLTR
                     RDLLRFLKIYRDRPLREVLSVEADFWAAVVERARRLYAKPVPRPPSG"
     gene            complement(1450..2604)
                     /locus_tag="VKZ48_06305"
     CDS             complement(1450..2604)
                     /locus_tag="VKZ48_06305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLU77493.1"
                     /translation="MPKMRFAFCIFAYFPYGGLQRDCMQLLRECRRRGHDVDLYTGEW
                     EGAVPEDVSVHFVPSRALTNHGVNVNFARNLSKRLRDSRYDAVVGFNKMPGLDVYYAG
                     DVCYASRVAHFSSVRRALPRYRRLIDFERAVFAPESSTEIILLARSQQDDYVRCYGTP
                     AERFHIMPPDMPVDRLYKGDRHAARAAARERFQLAAEHRLILMVGSGFKAKGVDRAVR
                     AIAALPEALRDRSRLVIVGDDNKAPYLGLAKSLGVANRVEFAGGQADVAPYYLAADLL
                     IHPAYHEMTGTVLLEALGHGLPVLVTDTCGFAVHVQQAEAGEVLASPFDQRVLDERLA
                     AMLDSPMRAQWGDNGVGYMNRLPLGVRPQRAADVLEQVAAGRAKREAASS"
     gene            complement(2816..2891)
                     /locus_tag="VKZ48_06310"
     tRNA            complement(2816..2891)
                     /locus_tag="VKZ48_06310"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2856..2858),aa:Lys,seq:ttt)
     gene            complement(2954..4069)
                     /locus_tag="VKZ48_06315"
     CDS             complement(2954..4069)
                     /locus_tag="VKZ48_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020205606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HLU77494.1"
                     /translation="MNEVDPATLVPYVQWGGFLLAFVFGFVANKTHFCTMGAVSDIIN
                     MGSWARMRMWLLAIAVAILGTCALQVSGLIDTRNSIYTSSNFLWLSHVVGGLLFGVGM
                     TLGSGCGSKTLIRVGTGNLKSLIVVVFMAIAAYMTLRGLFGAFRVEYLDAIAVQLPTN
                     QDLPSLLAQFTGAARDRVALLSALIAAALLLVFVFAHPDGRDKDVLLGGIVTGLVVVG
                     GWYVSGTLGYVPEDPESLQEAWVATNTGRMESLSFVAPQAYFLEWLMLWTDASLLLSF
                     GILLALGVVAGSMAYALLSRTFRLESFRDPGDMLNHMLGGILMGFGGITALGCTIGQG
                     ISGFSTLALGSILSFAAIVTGSALTMKYQYWRLMREA"
     gene            complement(4095..4784)
                     /gene="queC"
                     /locus_tag="VKZ48_06320"
     CDS             complement(4095..4784)
                     /gene="queC"
                     /locus_tag="VKZ48_06320"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HLU77495.1"
                     /translation="MNPASKAVVLVSGGLDSATVLAMARAQGYACHALSVDYGQRHRA
                     ELEAAARVAAALGAHVHKAVSVDLSAFGGSALTDREIDVPVDGVSTEAIPITYVPARN
                     TIMLSLALAWAEVLGARDIWYGANAVDYSGYPDCRPEYVRAYEAMANLATKAAVEEGR
                     RLTIHAPIIAMSKADIIRQGIALGVDYSLTVSCYQADDAGRACGRCDACRLRRAGFEA
                     AGVPDPTRYVL"
     gene            complement(4837..5568)
                     /gene="ybgF"
                     /locus_tag="VKZ48_06325"
     CDS             complement(4837..5568)
                     /gene="ybgF"
                     /locus_tag="VKZ48_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HLU77496.1"
                     /translation="MTRYAGILTAALLTVYGATAQAALFSDDEARRAVNELRARVDAQ
                     QRTLDQMASGGALMNLADSIDALRREMAELRGQVEVLHARLDLLDKRQKDLYVDLDNR
                     LRKFEQVRAEQDQAAQAAAAEQQAYEAGLAHFKANNYGAAIQALQAFQNEYPQSELAP
                     SAQYWIGNAYYALRDYKAAIAAQDKVVQTWPDNPKAPDALLNKASSQAESGDLAASRK
                     TLQSLVSKYPNSPAAEQAKQRLAKR"
     gene            complement(5600..>6123)
                     /gene="pal"
                     /locus_tag="VKZ48_06330"
     CDS             complement(5600..>6123)
                     /gene="pal"
                     /locus_tag="VKZ48_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HLU77497.1"
                     /translation="LMKKTLAIAAFALLFAGCASQPTTDGAPVQEGATPSTGQPSTGA
                     VQGSGVAGDPLNDPNHPMYRQLQARSVYFDFDSDAIKSEFVPVIEAHARFLRDNPNRK
                     VLIQGHADERGSREYNLGLGQRRADAVKSRLTLLGAKSSQVESTSLGEEKPRCTESTE
                     SCWAENRRGDLVY"
BASE COUNT         1219 a         1994 c         1896 g         1014 t
ORIGIN      
        1 caacgactgc attcactagg agaacgatat tcaacttcgc gagccgcgcc agtctttctc
       61 tgtggctgat gccggcatcc cacagcacga ccagcggcat gaaaagaaga aacgcgatgc
      121 cctcgatacg gaaaagcgtc gccaccgtca ttgcgagccc gaaacccagc gccagacgca
      181 gccgcggggt gagctgaaac ttgaaaaagg ccaacaggcc gaccaggtag aaggcccagt
      241 agccgacgtc ccggataacg aaggcgcgat agctgttcag cgagggcagc gcaagaatga
      301 gtatcgcggc aatccaaagc gtacgccggt ccgcgccgaa cagcttcaca atcgacagga
      361 aggtccatac cacaacggca tacagtgcgc cgttcaccac gtacgcgacg atgtccgtat
      421 ccaggccgct cacatagtgc agggcgccga tcagcgccgc atagagcggc catcgataaa
      481 tcgtgacccc ctgtcgccag tctccgtccg cgaacgcttc ggcgcttttc agatagagaa
      541 tgccgtcgcc gttgatgacg ttgtccgagt aagtcagcca gactgacagc aggatgctga
      601 ctgataccgc cacaagctcg atcggaatat tttttatgct tctcataact gcgaatcagc
      661 ccgagggcgg tcggggaacc ggcttggcat agagccggcg tgccctttcg acaactgccg
      721 cccagaagtc cgcctcgaca gacaatacct cgcgaagcgg gcggtcgcga tagatcttca
      781 ggaaacgcaa cagatcgcgt cgtgtcaggc cgatatccat cgccgaaaaa taaagtccgc
      841 cgagatcctt gatcagccag cggcgaggca ccttccggcg cacttgtgca cggtgaagat
      901 cgatcagcac cagccgccga tcggccccgg acaaggggca caggaagtgg cacagatagt
      961 aatcgcggtg attgaccccg ctctgatgca gcgcccggga aatgagggcg acttcacgaa
     1021 gcagggcctg cttgtcggag aacccgggcg gattctcggg ccaacgaccg caatgatcct
     1081 cgagcgaaac ggcctgcccc acatcctccg tgacgaggaa ggaatccatc gccgccgggt
     1141 tcctgccccg tctgccatag gccgcaatca taggtgcgcg gataccggcc gcctccaatc
     1201 gtcgaatcgc acgccactcg ttcatggcac ccagcaccgg caaccgcagg gtacagaggt
     1261 tcttgaggat ttcgacccag ccgacgccgc gatgcgcctt gacgaagtac gacttcgcgc
     1321 ctgcctggaa gcgtaccgtg cggcggctcg caatttcccg gaacacctcg ccgggcatgt
     1381 tcatgaacat gtcgaacgaa acgtctccac ccagcgcctc tttcaatgcg ggagcgacgt
     1441 agaactccgt catgaggaag ccgcctccct tttcgcgcgc cctgccgcga cctgttcgag
     1501 cacgtccgcc gcgcgctgcg ggcgcacgcc cagcggcaac cggttcatat agccgacgcc
     1561 gttgtcgccc cattgcgccc gcatcggcga atccagcatt gcagccaggc gttcgtccaa
     1621 tacccgctga tcgaaaggcg aggcgagcac ctcacccgcc tccgcctgct gcacatgcac
     1681 cgcgaaaccg cacgtatccg tcaccagcac cggcagtccg tggcccagcg cctcgagcag
     1741 cacagtgcct gtcatttcgt ggtacgcggg atggatcagc agatccgccg ccagatagta
     1801 gggtgcgacg tcggcctgtc cgcccgcgaa ttcgacgcga ttcgcgacac cgagcgattt
     1861 tgccagcccg agatagggcg ccttgttgtc gtcgcccacg atcacgagcc gggatcgatc
     1921 ccgcagcgct tcgggcaaag ccgctatcgc acgaacggcg cgatcgacac ccttggcctt
     1981 gaagcctgaa cccaccatca ggatcaggcg gtgctcggcc gccagttgaa atctttcccg
     2041 ggcggcagcc ctggcggcat gtcgatcgcc cttgtagagg cgatcaacgg gcatatcggg
     2101 cggcatgatg tgaaagcgtt cggccggggt gccgtagcaa cgcacgtaat cgtcctgctg
     2161 actgcgtgcc agcagaatga tctctgtgga agattcgggc gcgaacacgg cgcgctcgaa
     2221 atcgatgagc cgccggtagc ggggcagcgc acgtctcacg gacgaaaaat gcgcaacccg
     2281 gctcgcgtaa cagacgtcgc cggcgtaata cacatccagg ccgggcatct tattgaatcc
     2341 gaccaccgcg tcgtaacggc tatccctgag ccgcttcgac aggttgcggg cgaagttgac
     2401 gttgacgccg tgattggtca gcgcacggga gggcacgaaa tgaacggaga cgtcctcggg
     2461 cacggcgcct tcccattcgc ccgtatacag atcgacgtcg tgccccctgc gccgacactc
     2521 gcgcagcaac tgcatgcagt cgcgctgcag ccccccgtat gggaagtagg caaagatgca
     2581 gaaggcgaat ctcatcttcg gcataccgcg ctgcgggatc gaacagatcc agagggttcg
     2641 aggcagcgga ggaacggcac gagcgcagct atcgtggcga ccgcggcagt cgaaatcgca
     2701 ttccgcatca caaatccctg ctccgcgccg catgtccatt gcggcatccg gcgctccgcg
     2761 agcaccgcgc tcaccaaaaa caaaggggtt cgcctgcgcg aaccccttct ggtactggta
     2821 gggcgtgcgg gattcgaacc tgcgaccaac ggattaaaag tccgctgctc taccaactga
     2881 gctaacgccc cgaaagaggc gggattttaa gtgagcggcg atgcccggtc aacgcttatg
     2941 cagcccgaat cgctcatgct tcgcgcatca gccgccaata ctggtatttc atcgtgagcg
     3001 cggagccggt cacgatagcg gcgaagctca gtatcgagcc aagtgcgagc gtggagaaac
     3061 cgctaattcc ctgaccgatg gtgcagccca gcgcggtgat gccgccgaag cccatcagaa
     3121 tgccgccgag catgtggttg agcatgtcgc ccggatcgcg gaaggattcg agccggaagg
     3181 tgcggctcag cagcgcatag gccatcgaac cggcgacgac gccgagcgcg agcagaatgc
     3241 cgaagctcaa cagcagactc gcgtccgtcc agagcatcag ccattcgagg aaataagcct
     3301 gcggtgcgac gaaggagagc gactccatgc ggccggtgtt ggtcgctacc catgcttcct
     3361 gcagcgattc gggatcctcg gggacgtagc ccagcgtgcc gctgacatac cagccgccca
     3421 ccaccaccag tccggtgacg atgccgccga gcagcacgtc cttgtcgcgg ccgtcaggat
     3481 gcgcgaatac gaagacgagc aacagcgcgg cagcgatcag cgcactcagc agcgcgacgc
     3541 gatcgcgcgc cgcacccgtg aactgcgcga gcagcgaggg caggtcctga ttggtcggca
     3601 gttgcacggc gatcgcatcc agatattcga cacggaatgc gccgaacaga ccgcgcagcg
     3661 tcatgtacgc tgcgatcgcc atgaacacga cgacgatcag cgatttcaga ttgccggtgc
     3721 cgacccggat cagcgtcttt gaaccgcagc ccgagccgag cgtcatgccc acgccgaaca
     3781 gcagcccgcc caccacatgc gagagccaga ggaaattcga cgaggtgtag atggagttgc
     3841 gcgtatcgat cagtccggac acctgcagcg cgcaggtgcc caggatcgcc accgcgatgg
     3901 cgagcagcca catgcgcatg cgcgcccaac tgcccatgtt gatgatgtcc gacaccgcgc
     3961 ccatggtgca gaagtgcgtc ttgttggcga cgaagccgaa cacgaaggcg agcagaaagc
     4021 cgccccattg cacataggga accagcgtgg cgggatcgac ttcgttcatg ggacctcgat
     4081 gaaaatgcgg cgaattaaag cacgtagcgc gtcggatccg gcacgccggc cgcctcgaaa
     4141 cccgcacggc gcagccggca tgcgtcgcag cgtccgcagg cgcgccccgc atcgtcggcc
     4201 tgatagcacg agacggtgag cgagtaatcg acgcccagcg caatcccctg gcggatgatg
     4261 tccgccttgc tcatggcgat gatgggcgcg tgaatcgtca ggcgtcggcc ttcttcgacg
     4321 gccgccttgg tggcgaggtt cgccatcgcc tcataggccc gcacgtattc cggccggcag
     4381 tccggatagc cggagtagtc caccgcgttg gcaccgtacc agatgtcccg cgcgccaagc
     4441 acttccgccc acgccagcgc aagcgaaagc atgatggtgt tgcgcgccgg cacgtaggtg
     4501 atcggaatcg cctcggtcga tacgccgtcc accggcacgt cgatctcccg atcggtcagc
     4561 gccgaaccgc cgaaagcgga caggtcgacc gacaccgctt tgtggacgtg tgcaccgagg
     4621 gcggcggcca cgcgcgcagc ggcttccagt tcggcgcgat gacgctggcc gtaatcgacc
     4681 gacaacgcgt ggcaggcata gccctgcgcc cgcgccatgg cgagtacggt ggccgaatcg
     4741 aggccgcccg aaaccaggac gacagcttta gacgcgggat tcaatacgga attccatcaa
     4801 gcgtgcgccg cgacagggcg caggacgcat gatacgtcag cgcttggcga gacgctgctt
     4861 ggcctgttcg gcggccgggc tgttcggata cttggagacg aggctttgca gcgtcttgcg
     4921 cgaagcggcg agatcgccgg actcggcctg gctgctcgcc ttgttgagca atgcgtcggg
     4981 cgctttgggg ttgtccggcc aggtctgcac gaccttgtcc tgggcggcga tggccgcctt
     5041 gtagtcgcgc agcgcgtaat aggcgttgcc gatccagtac tgcgccgagg gcgccagctc
     5101 gctttgcgga tactcgttct ggaaagcctg cagggcctgg atcgcggcgc cgtagttgtt
     5161 cgccttgaaa tgcgccagtc cggcctcgta cgcctgctgc tcggccgcag cggcctgcgc
     5221 cgcctgatcc tgctcggcgc gcacctgctc gaacttgcgt aggcgattgt ccagatcgac
     5281 gtaaagatcc ttctgccgct tgtcgagcag atcgaggcgg gcgtgcagca cctccacctg
     5341 accgcgcagc tcggccatct cccgccgcaa ggcgtcgatg gaatcggcga ggttcatcag
     5401 cgcaccgccg ctggccatct gatccagcgt gcgttgctgc gcatccacgc gcgcgcgcag
     5461 ttcgttcacc gcgcgccgcg cttcgtcatc cgagaaaagg gccgcctgcg ccgttgcacc
     5521 gtacacggtc aacagcgcgg cggtcaggat gccggcataa cgggtcatca ctgttccctc
     5581 tcccgttcct tactacgact tagtaaacca gatcgccgcg gcggttctcg gcccagcagc
     5641 tttcggtcga ctcggtgcag cgcggcttct cttcgcccag gctggtcgat tcgacctggc
     5701 tcgacttcgc gcccagcagc gtgaggcggc tcttgaccgc gtcggcacgg cgctggccca
     5761 ggccgaggtt gtactcgcgg ctgccgcgct cgtcggcgtg accctggatc agcaccttgc
     5821 ggttcgggtt gtcacgcagg aaacgcgcgt gcgcttcgat gaccggcacg aattccgact
     5881 tgatggcatc gctgtcgaaa tcgaaataga cgctgcgcgc ctgcagctgg cgatacatcg
     5941 ggtggttggg atcgttcagc ggatcgccgg ccacaccgct gccctgcacc gcgccggtcg
     6001 aaggctggcc ggtcgaaggt gtcgcgcctt cctgcacggg cgcgccatcg gtagtgggct
     6061 ggctagcaca acctgcgaaa agcagcgcga aagctgcaat ggcgagggtc ttcttcatca
     6121 agt
//
LOCUS       DATGOU010000108        15469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_6096974, whole genome shotgun sequence.
ACCESSION   DATGOU010000108 DATGOU010000000
VERSION     DATGOU010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 15469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15469
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6096974"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..1180)
                     /gene="aroG"
                     /locus_tag="VKZ48_06335"
     CDS             complement(116..1180)
                     /gene="aroG"
                     /locus_tag="VKZ48_06335"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HLU77498.1"
                     /translation="MPVDDVRIREIKELSPPTHVLREFPSGPEAEKVVYETRQAIHRV
                     LHGGDDRLVVIVGPCSVHDVRAAMDYAERLLALKRELENDLILVMRVYFEKPRTTVGW
                     KGLINDPDLDGSFQINKGLRIARQLLLNVNELGIPAGTEFLDMITPQYYADLVSWGAI
                     GARTTESQVHRELASGLSCPVGFKNGTDGNVKIAVDAIKAASQPHHFLSVTKGGHSAI
                     VSTSGNEDCHVILRGGKTPNYDAASVDQACKDIASAGLASRLMIDTSHANSSKKPENQ
                     IPVNANIAAQIAGGETRIIGVMVESHLVAGRQDLIPGKPLTYGQSITDGCINWEDSLG
                     VLETLAEAVKQRRHVKDPDA"
     gene            1462..2460
                     /locus_tag="VKZ48_06340"
     CDS             1462..2460
                     /locus_tag="VKZ48_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLU77499.1"
                     /translation="MSLTPNLPVPHPSQVDIPIAGFKDIYDVESVERALQELPPGASE
                     ALKNTYEKMLKAGGQRFSVKPAGIPAMTHLYDELPNFAEVLDDIKKQIALCADSEDAL
                     ELTPMLLLGDPGIGKTHFGRRIAQMLSTGFGFVSMSSMTAGWVLSGSSSQWKNAKPGK
                     VFDTFLTGRYANPLIVVDEIDKAGGDSQYDALGALYTLLEHDTAAQFVDEFVDLPIDA
                     SSVLWIATANDASRIPEPILNRMNVYEIPQPDRDGAARIAQAIYSEIRNSRDWGSQFP
                     PQLDEDVLDRLAELSPREMRRAIMNGFGNAKLAGRDEVKVADVGEMRAAKKQRMGF"
     gene            complement(2620..5241)
                     /gene="alaS"
                     /locus_tag="VKZ48_06345"
     CDS             complement(2620..5241)
                     /gene="alaS"
                     /locus_tag="VKZ48_06345"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011884423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HLU77500.1"
                     /translation="MKSADIRSQFLQFFAAKGHQIVPSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYNRAVTAQKCVRAGGKHNDLENVGYTSRHHTFFEMLGNFSFGDYFK
                     RDAIAYAWELLTEVFRLPPEKLWVTVYEEDDEAYEIWNKQIGVPAERIVRIGDNKGAR
                     YASDNFWMMGDTGPCGPCTEIFYDHGPEVSGGPPGSPDEDGDRYIEIWNNVFMQYNRD
                     EQGVMHPLPKPSVDTGMGLERIAAVLQGVHSNYDIDLFRNLVAAAARETKQTEHDNPS
                     LRVLADHIRACGFLIADGVIPGNEGRGYVLRRIIRRAIRHGYKLGAREAFFHRMMPDL
                     VREMGEAYPELVANQSRVMDVLKQEEDRFFDTIEHGMSILEAELAQIAKSGTQTLNGE
                     TAFKLHDTYGFPLDLTADICRERNVTVDEAGFDAAMARQKEQARAAGKFKMALNLEFE
                     GEPTTFHGYEKLEEQGSIVALYKDGIAVDALEEGDMGVVVLDHTPFYAESGGQIGDRG
                     ELRGSGGILEVEDTQKIQAQVYGHHGVVKTGILRKGDRVLARVDAEARARTMRHHSAT
                     HLMHKALREVLGDHVQQKGSLVDPDKTRFDFTHHAPMTADEIRRVEQLVNAEILANAE
                     TAARIMPIEEAQKSGAVMLFGEKYGDEVRVLDIGSSRELCGGTHVARTGDIGLFKIVS
                     EGGVAAGIRRLEAVCGDVALAYVQREHALLMQTASELKAPLVELPTKIAQVMENVRAL
                     EREINRLKSKMAATQGDELAAQAEDVKGVKVLAASLDGADVKTLRETVDKLRDKLKSA
                     AIVLAASDGAKVSLIAGVTPDLTGKVKAGELVNFVAQQVGGKGGGRPDMAQAGGTQPD
                     KLPTALASVTKWAEERL"
     gene            complement(5336..6607)
                     /gene="egtB"
                     /locus_tag="VKZ48_06350"
     CDS             complement(5336..6607)
                     /gene="egtB"
                     /locus_tag="VKZ48_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HLU77501.1"
                     /translation="MSAVLQPIDPSTDLAARYRAVRDATMALTRPLSDEDCVVQSMPD
                     CSPAKWHLAHTTWFFETFILEAQAGYRPYAPAYRVLFNSYYQSVGAQHARPRRGLLTR
                     PSRAEVTAYRAAIDEQMDALLRRGVAREVAAIVELGLHHEQQHQELLLTDIKHLFGCN
                     PLRPAYRTTETVIADGVAPPLSWHAYPGGLAQFGHDGEGFAFDNEGPRHRAYLHPYTL
                     ASRAVTNAEFLAFIDEGGYEQPKYWLSAGWDIVNAQGWRAPLYWEQRDGQWWQHTLYG
                     LRPVAPHEPVCHISYYEADAYARYAGARLPTEYEWEAAAAGADMAGNFVESGALHPRA
                     ASAQGQADTPLQLYGDVWEWTASAYAPYPGYAPAQGALGEYNGKFMCSQQVLRGGSCV
                     TPASHIRATYRNFFPPDARWQFSGIRLARDL"
     gene            complement(6702..7145)
                     /gene="recX"
                     /locus_tag="VKZ48_06355"
     CDS             complement(6702..7145)
                     /gene="recX"
                     /locus_tag="VKZ48_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HLU77502.1"
                     /translation="MKKLSLRARAIDLLARREHSRSELARKLARHAEEGDDIDALLDD
                     LVARKLLSDERYAEARAHTLSRKYGAARIAHDLRMQGVDEEIVRRIADEARRTEVQRA
                     REAWQKRFGRLPESAEERARQMRFLAARGFSGEAVRQVLRTLGDD"
     gene            complement(7226..8245)
                     /gene="recA"
                     /locus_tag="VKZ48_06360"
     CDS             complement(7226..8245)
                     /gene="recA"
                     /locus_tag="VKZ48_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HLU77503.1"
                     /translation="MEENKSKALAAALAQIEKQFGKGSVMRMDSEAAPDVDVVSTGSL
                     GLDIALGVGGLPRGRVVEIYGPESSGKTTLTLQVVAEMQKLGGTAAFIDAENALDPQY
                     AQKLGVNINELLISQPDTGEQALEIADMLVRSGSVDLIVIDSVAALVPKAELEGEMGD
                     SLPGLQARLMSQALRKLTGTIKRTNTLVIFINQIRMKIGVMFGSPETTTGGNALKFYS
                     SVRLDIRRIGSIKKSDEVIGNETRVKIVKNKVAPPFREAIFDIMYGEGISREGEILEL
                     GVQHGIVEKAGAWYSYNKDRIGQGKDNVREYLREHPELAAEIEALVREKVGVRNPAAP
                     IAVEA"
     gene            complement(8481..8975)
                     /gene="pncC"
                     /locus_tag="VKZ48_06365"
     CDS             complement(8481..8975)
                     /gene="pncC"
                     /locus_tag="VKZ48_06365"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HLU77504.1"
                     /translation="MSDAELNALARRVGTALQARGLMLATAESCTGGGIACALTDIAG
                     SSAWFERGFVTYSNEAKQEQLGVSAATLAQHGAVSEPVALEMASGALRHSRAQAALSV
                     TGIAGPAGGTAQKPVGMVCFAWALPGQAPIAVTHHFKGDRAGVRRGAVAFALQGLLDR
                     LEAS"
     gene            complement(8972..9448)
                     /locus_tag="VKZ48_06370"
     CDS             complement(8972..9448)
                     /locus_tag="VKZ48_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014007673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HLU77505.1"
                     /translation="MRPSVSFMLAHPAHFVAMGFGTGLSPVGPGTVGTLLAIPLYAVL
                     LDRFTPLEVLALCVLLFSIGVWVCDRTNRDLGVDDHGSITWDEIVAFLAVLTFIPASL
                     NWQASAFVLFRLFDIMKPPPIRRIDTRVKGGIGTMLDDLLAAFYTLLVLAIFKIVL"
     gene            complement(9454..10428)
                     /gene="thiL"
                     /locus_tag="VKZ48_06375"
     CDS             complement(9454..10428)
                     /gene="thiL"
                     /locus_tag="VKZ48_06375"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HLU77506.1"
                     /translation="MVRPAPSPEFALIARYFTRPAKCALLGVGDDCALVRSGDEALAI
                     TTDMLVAGTHFLPDTDPHALGHKALAVNLSDLAAMGAEPAWFTLALALPGIDDDWLRP
                     FADGLLALADAHGIELVGGDTTRGPLTISVTAAGAVPLGEALRRDGAVAGDDVWVSGS
                     TGDAALALAHLQQRVVLDAADAAACLERLHRPQPRVALGRALRGVAAGAADVSDGLLA
                     DIGHILERSQCGADIEFERLPRSAALRACADQALAAACLVGGGDDYELVFTAPPERRA
                     AVEAAGEAAGVPVTRIGRIVDGEPVARLLDARGAPMRVERPGFDHFVG"
     gene            complement(10428..10868)
                     /gene="nusB"
                     /locus_tag="VKZ48_06380"
     CDS             complement(10428..10868)
                     /gene="nusB"
                     /locus_tag="VKZ48_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016344713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HLU77507.1"
                     /translation="MKSARRRAREYAVQGLYQWQLAGTRVGDIERSLAEDSEFAEADR
                     ALFVQLLHGASSNVEALEAALRPALDRAPDVLSPIERAILLLATFEFVHMPETPYRVV
                     INEAIELAKTFGGTDGHKYVNGVLDKLAPTLRPHEKAGARNRAQ"
     gene            complement(10905..11366)
                     /gene="ribH"
                     /locus_tag="VKZ48_06385"
     CDS             complement(10905..11366)
                     /gene="ribH"
                     /locus_tag="VKZ48_06385"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HLU77508.1"
                     /translation="MNEIAVRLEGAGIRVAIVRARFNENVGEAATAACTARLRELGVA
                     EQDITLLSVPGALEAPLALRALAQSGRYHALIALGAVIRGETYHFEIVSNESAAGITR
                     VQLDTGVPVANGILTCETDEQADVRAAVKGRDCAEVAIEMANLLRAVGVHR"
     gene            complement(11363..12466)
                     /gene="ribBA"
                     /locus_tag="VKZ48_06390"
     CDS             complement(11363..12466)
                     /gene="ribBA"
                     /locus_tag="VKZ48_06390"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HLU77509.1"
                     /translation="MQFATTAEIIEEIKAGRMVVLVDDEDRENEGDLVLAADFVTPEA
                     INFMAKYGRGLICLTLTEQRCRQLNLPLMVRDNRAPLGTAFTVSIEAATGVTTGISAY
                     DRARTVQAAVAPNAKPEDVVQPGHIFPLMAQNGGVLVRAGHTEAGCDLAQLAGLSPTA
                     VICEILKDDGTMARLPDLFEFARKHGLKIGSIADLINFRSAHESLVRRVTEREIDTAY
                     GRFRLIAYLEKLSGETHLALVQGEIRADEETLVRVHEPLSVLDLLDIGDSGHSWTVPQ
                     ALTHIARAGRGVMVLLNCAESAGQLIERVASSQASRNAPKRDLLTHGIGSQILRDLGV
                     GRMRLMAAERKMPSMAGWALEITGYVQPEGHQA"
     gene            12581..14146
                     /locus_tag="VKZ48_06395"
     CDS             12581..14146
                     /locus_tag="VKZ48_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLU77510.1"
                     /translation="MSRRVLHCLGCALLAAMLLVAPAGAADLRIAMAADVGSMDPHFA
                     NISPNVVVSSHVFETLTTVDADARLMPALAESWAAVGPTTWEFRLREDVRFHDGSPFT
                     AEDVLFSLQRPATIKNSPGGFTTFTKPIVRMEAVDRHTVRLHTAAPYAMVPYDLVSVF
                     IVSKRAAEGARSRDFDDGTAMIGTGPYRFVRFARGEAIDLARNDDYWGKRPEWEHVSL
                     RLIPSDPARIAALYAGDVDMIENVPPADAARLARDPRFRIERKISWRTIFLTLDQYRD
                     DSPFIDAGGKPNPLRDARVRRALSKAIDREAICAKVMDGFATPTANIVAPTVFGHAHS
                     LQAEAYDPAGAKALLAEAGYADGFELTLHGPNNRYINDELIVQAVAQMLARIGIRTRV
                     ETMPVNIYFPKARAESFSVALLGWGSFSGDLALRSLFMTENATQGTGAWNWGRYSNPE
                     VDELVRRALGALEPAERERLTREATVRAMGETALIPLYHQIASWALRRDLRYIARTDE
                     YSFAFDVHTETTP"
     gene            14143..15117
                     /locus_tag="VKZ48_06400"
     CDS             14143..15117
                     /locus_tag="VKZ48_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLU77511.1"
                     /translation="MTAYLIRRALQALLVLLVMSALLFVGIYAIGNPVEILISPQADQ
                     RDIALATQALGLDQPLWRQYFAFVEHALRGDLGHSFVYNVPALELVLSRLPATLELAV
                     AAMLIAIVIGLPLGIWAGMRPDSWLGKTIMAGSILGFSLPTFWVGLLLIMFFGVQLGW
                     LPASGRGTTTDWFGVPVSFTTLDGLAHLALPAFNLALFKLSLLIRLARNGVRDALHQD
                     YVRTARAKGLHEGQVVRRHVLKNIMLPIVTVIGLEFGSLVAFAVVTETIFAWPGAGKL
                     LIDAINVLDRPVVVAYMLLTVLLFVLINFVVDVLYTALDPRVRLAGNQ"
     gene            15114..>15469
                     /locus_tag="VKZ48_06405"
     CDS             15114..>15469
                     /locus_tag="VKZ48_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLU77512.1"
                     /translation="MNALRRFGASRLALIAATVLALLVFVAVFAPVIAPQDPYDLAQL
                     DVMEGRLPPGAASLDQTRHYWLGTDEQGRDMLSAIFYGLRSSLYVGVTATLCALALGS
                     ILGVAAGYFGGWFESVA"
BASE COUNT         2551 a         5102 c         5030 g         2786 t
ORIGIN      
        1 gcaaaggcgc accgattcgc atcgacggaa tcccgggcat cttcgaatcg tccattccaa
       61 tcgtccaaca aaaagccccg gcatgccggg gctttttcat gtcgggatac gcggatcacg
      121 catccgggtc cttcacgtga cggcgctgct tgaccgcttc cgccagcgtc tccagcacgc
      181 ccaggctgtc ttcccagttg atgcagccgt cggtgatcga ctggccatag gtcagtggct
      241 tgccggggat caggtcctga cggccggcca ccagatgcga ttcgaccatc acgccgatga
      301 tgcgcgtttc gccgccggcg atctgcgcgg cgatgttggc gttgaccgga atctggttct
      361 cgggcttctt cgagctgttg gcatgcgaag tatcgatcat caggcgcgac gcgaggccgg
      421 ccgaagcaat gtccttgcag gcctggtcga cgctggcagc gtcgtagttc ggcgtcttgc
      481 cgccgcgcag gatcacgtgg caatcctcgt tgccgctggt cgacacgatc gccgaatggc
      541 cgcccttggt caccgacagg aagtgatgcg gctgcgacgc cgccttgatc gcgtcgaccg
      601 cgatcttgac gttgccgtcg gtgccgttct tgaagccgac cgggcacgac agtcccgaag
      661 ccagttcgcg atgcacctgc gattcggtcg tgcgcgcgcc gatcgcgccc cagctcacca
      721 ggtcggcgta gtactgcggc gtgatcatgt ccagaaactc ggtaccggcc ggaatgccca
      781 actcgttcac gttgagcagc agctggcgcg caatgcgcag ccccttgttg atctggaagc
      841 tgccgtcgag gtcgggatcg ttgatcaggc ctttccagcc gaccgtggtg cgcggctttt
      901 cgaagtagac gcgcatgacg aggatcagat cgttttcgag ttcgcgcttc agcgcgagca
      961 ggcgctcggc atagtccatc gccgcgcgca cgtcatgcac cgagcagggg ccgacgatga
     1021 ccaccagccg gtcgtccccg ccatgcagta cgcgatgaat ggcctgacgc gtttcgtaca
     1081 ccaccttttc ggcttccggc ccgctcggaa attcgcgcag cacgtgcgtg gggggcgaaa
     1141 gttccttgat ctcgcggatg cgtacgtcgt ctacgggcag gctcatattg gcactgggaa
     1201 tgctgcttag tttggcggtg gacatggtta ctgctttgat tcgtgagggc gaattatatc
     1261 gcaacaaggc tatgcttccg gcattggttt ccttcattag cctcatagaa ccatcgatcg
     1321 ccatgctttg tcactagcaa gcggcggagc cggctgccag cccaattcgc ataaacaccg
     1381 ggcttttgtc gcgtagctcc gtttattgcc ttcgtgtacg cgtggtagcg tttggaccag
     1441 cgctgcaaaa ggaggtgagc catgtctttg acacccaacc ttcccgttcc gcacccgtca
     1501 caggtcgaca ttccgatcgc cggcttcaag gacatctacg atgtcgagag cgtggagcgg
     1561 gcattgcagg aactgccgcc gggcgcgagt gaggcgctca agaacaccta cgagaaaatg
     1621 ctcaaggcgg gcggacagcg cttctcggtc aaaccggcgg gcattcccgc catgacgcat
     1681 ctttacgatg aactgcccaa cttcgccgaa gtgctggacg acatcaagaa gcagatcgcc
     1741 ctttgcgccg acagcgagga cgcgctggaa ctgacgccga tgctgctgct cggcgacccg
     1801 ggcatcggca agacgcactt cggccgccgc atcgcccaaa tgctctcgac cggattcggc
     1861 ttcgtttcca tgagttcaat gaccgccggc tgggtgctgt cgggctcgtc ctcacagtgg
     1921 aagaacgcca agccaggcaa ggtgttcgac accttcctca ccggccggta cgccaatccg
     1981 ctgatcgtgg tggatgagat cgacaaggcc ggcggcgact cgcaatacga cgcgctcggc
     2041 gcgctctaca cgctgctcga gcacgacacc gccgcgcagt tcgtggacga gttcgtcgat
     2101 ctgccgatcg acgcgtccag cgtgctgtgg atcgccaccg cgaacgacgc ctcgcgcatt
     2161 cccgagccga tcctcaaccg catgaacgtg tacgaaatcc cgcagccgga tcgcgacggc
     2221 gccgcacgca tcgcccaggc gatctacagc gaaatccgca attcgcggga ttggggcagc
     2281 cagttcccgc cgcaactgga tgaggacgta ctcgaccggc tggccgaact gtcgccgcgc
     2341 gagatgcggc gcgccatcat gaacggcttc ggcaacgcca aactggcggg ccgcgacgaa
     2401 gtcaaggtcg cagacgtggg cgaaatgcgc gctgcgaaga agcagcgaat ggggttttaa
     2461 aaaaactgaa ccacagaggc acagaggaac cgtgcggaac gcttctcgct cggaagcgga
     2521 tttcgaaagt ccgcgccttc acaaagcaca agcgggccgt gcaaccttac tcaggtcttt
     2581 gcttttctct gtgtctctgt gcctctgtgg tgaagatgtt taaagccgct cttccgccca
     2641 cttcgtcacc gaggcgagcg ccgtcggcag cttgtccggt tgcgtaccgc cggcctgcgc
     2701 catgtccggg cgtccaccgc ccttgccgcc gacctgctgt gcgacgaagt tgaccagttc
     2761 gccggccttc accttgccgg tgagatcggg cgtaaccccg gcgatcaggc tgaccttggc
     2821 gccgtcgctg gcggcgagca cgatggctgc ggacttcagc ttgtcgcgca gtttgtccac
     2881 cgtttcgcgc agcgtcttga cgtcggcgcc gtccagcgac gcggcgagca ccttcacgcc
     2941 tttgacgtcc tcggcctgcg cggcaagttc gtcgccctgg gtagcggcca tcttggattt
     3001 cagacggttg atctcgcgct ccagcgcgcg cacgttttcc atcacctgcg cgatcttcgt
     3061 cggcagttcc acgagcgggg ccttcaattc actcgccgtc tgcatcagca acgcatgctc
     3121 acgttgcaca taggccagcg ccacgtcgcc gcatacggcc tcgaggcggc ggatgccggc
     3181 ggccacgccg ccttcggaca cgatcttgaa caggccgatg tcgcccgtac gcgcgacgtg
     3241 ggtgccgccg cacagttcgc gcgagctgcc gatgtcgagc acgcgcactt cgtcgccgta
     3301 cttctcgccg aagagcatga ccgcgccgga cttctgcgct tcctcgatgg gcatgatgcg
     3361 cgcagcggtt tccgcgttcg ccaggatctc cgcgttgacg agctgttcga cgcggcgaat
     3421 ctcatccgcc gtcatgggcg catggtgcgt gaagtcgaag cgcgtcttgt cgggatcgac
     3481 cagcgagccc ttctgctgca catggtcgcc cagcacttca cgcagggcct tgtgcatcag
     3541 gtgcgtagcg gagtggtgac gcatggtgcg cgcacgcgcc tcggcgtcga cgcgcgccag
     3601 cacgcggtcg cctttgcgca ggatgcccgt cttcaccacg ccgtgatggc cgtacacctg
     3661 cgcctggatc ttctgcgtgt cttccacttc gagaatgccg ccgctgccgc gcagttcgcc
     3721 gcggtcgccg atctggccgc ccgactcggc gtagaaaggg gtgtgatcga gcaccaccac
     3781 gcccatgtcg ccttcctcga gcgcgtcgac ggcgataccg tccttgtaga gcgcaacgat
     3841 gctgccctgc tcttcgagtt tctcgtaacc gtgaaaagtg gtcggctcgc cctcgaactc
     3901 gaggttgagc gccatcttga acttgccggc ggcgcgcgcc tgttccttct ggcgcgccat
     3961 ggcggcgtcg aagccggcct cgtcgacggt cacattgcgc tcgcggcaga tgtcggccgt
     4021 caggtcgagc gggaaaccgt aggtgtcgtg cagcttgaaa gcggtctcgc cgttcagcgt
     4081 ctgcgtaccc gacttggcga tctgcgcaag ctccgcttcg aggatcgaca tgccgtgttc
     4141 gatggtgtcg aagaaacggt cttcctcctg cttcagcacg tccatcacgc gcgactggtt
     4201 ggcgacgagt tcgggatagg cctcgcccat ctcgcgcacc aggtccggca tcatgcgatg
     4261 gaagaaggcc tcgcgcgcgc cgagcttgta gccgtggcga atggcgcggc ggatgatccg
     4321 gcgcagcacg tagccgcggc cctcgttgcc ggggatgaca ccgtcggcga tgaggaagcc
     4381 gcaggcgcgg atgtgatcgg ccagcacacg cagcgacggg ttgtcgtgtt ccgtctgttt
     4441 cgtctcccgc gccgccgccg caacgagatt gcggaacagg tcgatgtcgt agttcgaatg
     4501 cacgccctgc aacaccgcgg cgatgcgctc cagccccatg ccggtatcga cgctcggctt
     4561 gggcagcgga tgcatcacgc cctgctcgtc gcggttgtac tgcatgaaga cgttgttcca
     4621 gatttcgata taacggtcgc cgtcctcgtc gggcgatccg ggcgggccgc cggagacttc
     4681 cggtccgtga tcgtagaaaa tttccgtgca gggaccgcag ggaccggtat cgcccatcat
     4741 ccagaagttg tcggaggcgt agcgcgcgcc cttgttgtcg ccgatgcgca cgatccgctc
     4801 ggcgggcacg ccgatctgct tgttccagat ctcgtaggct tcgtcgtcct cctcgtacac
     4861 cgtcacccac agcttctcgg gcgggagcct gaagacttcg gtcagcagtt cccacgcgta
     4921 ggcgatggcg tcgcgcttga aataatcccc gaagctgaag ttgcccagca tttcgaagaa
     4981 ggtgtgatgg cgcgaggtat agccgacgtt ctccaggtcg ttgtgcttgc cgcccgcgcg
     5041 cacgcatttc tgcgccgtca ccgcgcggtt atagggacgc ttgtcgaagc cgaggaacac
     5101 gtccttgaac tggttcatcc cggcgttggt gaacagcagc gtggggtcgt cgtgcggcac
     5161 cagcgaactg gagggaacga tctgatgccc cttggcggcg aagaactgga gaaactgact
     5221 gcggatgtcg gccgacttca tggatatgcg ccctcaggcg ccgttgcaaa tgtctgaaag
     5281 gcggacattt tcgcccggaa tgacaggcgc gcgcaaacgc gcctgcctat gacgctcata
     5341 ggtcgcgggc aaggcggatg ccgctgaact gccagcgtgc atcgggcgga aagaagttgc
     5401 gataagtcgc gcggatatgc gaggcgggcg taacgcagga accgccgcgc agcacctgct
     5461 ggctgcacat gaatttgccg ttgtattcgc ccagggcacc ctgcgcgggc gcatagccgg
     5521 gataaggcgc gtaagcgctg gcagtccatt cccatacgtc gccgtagagt tgcaacggtg
     5581 tatccgcctg cccttgcgcc gaagccgcac gcggatgcaa ggcgcctgat tcgacgaagt
     5641 tgcccgccat gtccgctccc gccgcagccg cctcccattc gtactcggtc ggcagtcggg
     5701 cgcccgcgta gcgcgcatag gcgtcggctt cgtagtaact gatgtgacag accggttcgt
     5761 gcggcgcgac cggccgcagg ccgtacaggg tgtgctgcca ccattgcccg tcccgctgct
     5821 cccagtacag cggcgcacgc cagccctgcg cattgactat gtcccacccg gcggacagcc
     5881 agtatttcgg ctgctcatag ccgccttcgt cgatgaacgc gagaaactcg gcattggtca
     5941 ccgcgcgcga ggccagggtg tagggatgca gataggcgcg gtggcgcggg ccctcgttat
     6001 cgaaggcgaa accttcgccg tcatgaccga actgcgcgag tccgccggga taggcgtgcc
     6061 aggacagcgg cggcgcaacg ccgtccgcaa tcacagtctc cgtcgtccga tatgccggcc
     6121 ttaaaggatt gcagccgaac agatgcttga tgtcggtgag cagcaattcc tgatgctgct
     6181 gctcgtggtg gagccccagt tcgacgatgg cggccacttc ccgagcgacg ccgcgacgca
     6241 gcagcgcgtc catttgttca tcgatggcgg cgcgataggc cgtcacttcc gcacgcgaag
     6301 gccgtgtcag cagcccgcgg cgcggccgcg catgctgcgc accgacactc tggtagtagg
     6361 aattgaacag cacccggtag gccggcgcat acggcctgta accggcctgc gcttcgagga
     6421 taaaggtctc gaaaaaccag gtcgtatgcg ccagatgcca tttcgccgga ctgcagtcgg
     6481 gcatggactg gacgacgcag tcttcgtcgc tcaacggacg ggtcaacgcc atcgtggcgt
     6541 cccgaacggc gcgatagcgc gccgcgagat cggtggacgg gtcgatgggc tgcaaaactg
     6601 ccgacatggc gcctcctgcg cttggaatcc atgtactcgt gcaacgtgcg ggccgacgac
     6661 aggcgcgcgg ccggaccgtt gggccgttcg cccggccggt gttagtcgtc gcccagggtg
     6721 cgcagcacct gccggaccgc ttcgccgctg aaaccgcgtg cggccagaaa acgcatctgc
     6781 cgcgcccgct cctcggccga ctcgggcagg cggccgaacc gtttttgcca cgcttcgcgc
     6841 gcgcgctgca cttccgtacg ccgggcctcg tcggcgatgc gccggacgat ttcctcgtcc
     6901 acgccctgca tgcgtagatc gtgcgcgatg cgcgcggcgc catacttgcg tgacagcgta
     6961 tgcgcacgcg cctcggcata gcgttcgtcg gaaagcagct tgcgcgcaac caggtcgtcg
     7021 agcaaggcgt cgatgtcgtc gccttcttcc gcatgccggg cgagtttgcg tgccagctcg
     7081 ctgcggctgt gttcgcgccg ggccagcagg tcgattgcgc gcgcgcgcag actgagtttc
     7141 ttcacgctac ggcccggctc ccgtcacagg ctaaaacgcc gggagcaacg ctctcgcgac
     7201 actcccggtg gacggccggc aaaactcagg cttcgaccgc gatcggcgcc gccgggttgc
     7261 gcacgccgac cttctcgcgc accagcgctt cgatctcggc ggccagttcc ggatgctcgc
     7321 gcaggtattc gcgcacattg tccttgccct ggccgatacg gtccttgttg tacgagtacc
     7381 aggcgcccgc cttttcgacg atgccgtgct gcacgcccag ttcgaggatt tcgccttccc
     7441 gcgaaatgcc ttcgccgtac atgatgtcga aaatcgcttc gcggaagggc ggcgcgacct
     7501 tgttcttcac gatcttgacg cgcgtctcgt tgccgatgac ctcgtcggac ttcttgatgg
     7561 agccgatgcg gcggatatcc agacgcaccg aggagtagaa cttgagcgcg ttgccgccgg
     7621 tggtggtctc ggggctgccg aacatgacgc cgattttcat gcggatctgg ttgatgaaaa
     7681 tcaccagcgt gttggtgcgc ttgatcgtgc cggtcagctt acgcagcgcc tgactcatca
     7741 gacgggcctg caaaccgggc agcgagtcgc ccatctcgcc ttccagttcc gctttgggca
     7801 ccagcgcggc gaccgagtcg atcacgatca ggtcgaccga acccgaacgc accagcatgt
     7861 cagcgatttc cagcgcctgt tcgccggtgt ccggctggga gatgagcagt tcgttgatgt
     7921 tgacgccgag cttctgcgca tactgcgggt cgagcgcgtt ttccgcgtcg ataaaagccg
     7981 cggtgccgcc cagcttctgc atctcggcca ccacctgcag cgtcagtgtg gttttgcccg
     8041 aggactcggg tccgtagatt tcgacgaccc ggccgcgcgg cagcccgccc acgccaagcg
     8101 caatgtccag accgagcgag ccggtggaca cgacgtccac gtcgggcgcg gcttcggaat
     8161 ccatgcgcat gacggacccc ttgccgaact gcttctcgat ttgcgccagc gcagcggcca
     8221 gggccttgga tttgttctct tccatgatgt tcgcgaggtc cttatcagag tgcacggtat
     8281 tctcccatga tcaagcgtcc gaacaagggg cggtcgcccc actctctagc cgcagcgacg
     8341 gccgcgcgct tctccatggg aaggcagtat gcgcagaccg ctggctcagg agatgagccg
     8401 cgcgcatctt aacacgctac ctgtggatgt atacagtacc gcttatcggc cggaattacc
     8461 gcatcgcgtc cgggggcgcc tcaggaggct tcgaggcggt cgagcagtcc ctgcagtgcg
     8521 aaggcgacgg cgccgcgccg caccccggca cgatccccct tgaagtgatg cgtgacggcg
     8581 atcggcgcct gccccggcag cgcccaggcg aagcaaacca tgcccaccgg tttctgcgcc
     8641 gtaccgcccg cgggaccggc gatgcctgtc acggacaaag ccgcctgcgc acgactgtgt
     8701 cgcaacgcgc cgctcgccat ttccagcgcg acgggctcgc tcaccgcgcc gtgctgcgcg
     8761 agcgtggcgg cgctgacgcc gagctgctcc tgcttcgctt cgttggaata ggtgacgaag
     8821 ccgcgctcga accaggcgga actgccggcg atgtcggtca gcgcgcaggc gataccgcca
     8881 ccggtgcagg attcggccgt ggcaagcatc aagccgcgcg cctgcagtgc ggtgccgaca
     8941 cgccgcgcaa gcgcattcag ttccgcgtcg ctcatagcac gattttgaaa atcgccagca
     9001 ccagcagcgt gtagaacgcc gccagcagat cgtcgagcat cgtaccgatg ccgcccttca
     9061 cgcgcgtatc gatgcgccgg atgggcggcg gcttcatgat gtcgaacagc cggaacagca
     9121 cgaaagcgct ggcctgccag ttcagcgatg ccggaatgaa agtcagcacc gccaggaagg
     9181 cgacgatctc gtcccaggtg atcgagccgt ggtcgtccac gcccaggtcg cgattggtgc
     9241 ggtcgcagac ccacacaccg atggaaaaca gcagcacgca cagcgccagc acttccagcg
     9301 gcgtgaagcg atcgagcagc accgcgtaga gcggaatggc gagcagcgtg ccgacggtgc
     9361 cggggccgac gggcgacagc cccgtgccga aacccatcgc gacgaagtgg gcgggatggg
     9421 ccagcatgaa ggagacgctt ggacgcatga tggtcagccg acgaagtgat cgaagccggg
     9481 acgctcgacg cgcatcggcg cgccgcgggc gtccagcaaa cgggccaccg gttcgccatc
     9541 gacgatgcgg ccgatgcgcg tgaccggcac gccggccgcc tcgcccgccg cttcgacagc
     9601 agcgcgacgt tcgggcggcg cggtgaagac caactcgtaa tcgtcgccgc cgccgacgag
     9661 acaggcggcg gcgagcgcct gatccgcgca ggcacgcagg gccgccgaac gcggcagacg
     9721 ctcgaactcg atgtccgcgc cgcattgcga gcgttcgagg atatgcccga tatcggcaag
     9781 caggccgtcg gacacgtcag ccgcaccggc ggcgacaccg cgcagcgcgc ggccgagcgc
     9841 cacgcgcggc tgcggccgat gcaggcgttc gaggcaggcc gccgcgtccg ccgcatcgag
     9901 cacgacgcgc tgttgcagat gcgcaagcgc aagcgccgca tcgccggtcg agccggacac
     9961 ccacacgtcg tcgcccgcca cggcgccatc gcgtcgcaat gcttcaccca ggggtaccgc
    10021 gccggcggcg gtcacggaaa tggtgagcgg cccgcgcgtg gtgtcgccgc cgaccagttc
    10081 gatgccgtgc gcatcggcca gcgccagcag gccatcggcg aacgggcgga gccagtcgtc
    10141 gtcgatgccg ggcaaggcga gcgcgagggt gaaccacgcg ggttcggcgc ccatggcggc
    10201 gaggtcggag agattgaccg ccagcgcctt gtgtcccaag gcatgcggat cggtatcggg
    10261 cagaaagtgg gtgccggcca ccagcatgtc ggtcgtgatg gccagcgcct catcgccgct
    10321 gcgcaccaac gcacagtcgt cgcccacgcc cagcagcgcg cactttgccg ggcgcgtgaa
    10381 gtaacgcgcg atgagcgcga attcgggcga tggcgccggc cgcaccatca ctgcgcgcgg
    10441 ttgcgcgccc ccgctttctc gtgcggacgg agtgtggggg cgagcttgtc gagcacgccg
    10501 ttgacgtact tgtgaccgtc cgtgccgccg aaggtcttgg ccagttcgat tgcttcgttg
    10561 atcaccacgc gatagggcgt ctcgggcatg tgcacgaact cgaaagtggc gagcagcagg
    10621 atcgcccgct cgatgggcga gagcacatcc ggcgcgcggt cgagcgccgg acgcagcgct
    10681 gcctccagcg cctcgacatt ggacgaggcg ccgtgcagca actggacgaa cagcgcccgg
    10741 tcggcctcgg caaactcact gtcctcggcg aggctgcgct cgatatcgcc gacccgtgtg
    10801 ccggccagtt gccattgata aagcccctgc accgcatatt cgcgggcgcg gcggcgtgcc
    10861 gatttcatgc ttctgttctc catgcaggcc tgcggccggc gcgcctagcg atgcacgccc
    10921 acggcgcgca gcagattggc catttcgatc gcgacctcgg cgcagtcgcg ccccttcacc
    10981 gcggcgcgca catcggcctg ttcgtcggtc tcgcaggtca ggatcccgtt ggcgacgggc
    11041 acgccggtat cgagttgcac gcgggtgatg cccgcggcgg attcgttgct gacgatttcg
    11101 aagtggtagg tctcgccgcg gatcaccgcg ccgagcgcga tgagcgcgtg atagcggccg
    11161 ctctgcgcca acgcgcgcag cgcgagcggc gcttccagcg cacccggcac ggaaagcaga
    11221 gtgatgtcct gctccgccac gccaagttcg cgcaggcgcg cagtgcaggc cgccgtcgct
    11281 gcctcgccca cgttttcgtt gaagcgggcg cgcacgatgg cgacgcgaat gccggcgcct
    11341 tcgagccgta cggcgatttc attcatgctt ggtgaccttc aggttgcaca taaccggtga
    11401 tttccagcgc ccagccggcc atgctgggca ttttgcgttc cgccgccatg aggcgcatcc
    11461 tgcccacgcc cagatcgcgc aggatctgcg agccgatgcc gtgggtcagc agatcgcgct
    11521 tgggcgcgtt gcgcgacgcc tggctggagg ccacgcgctc gatcagttgc cccgcgcttt
    11581 cggcgcagtt gagcagcacc atcacgcccc ggcctgcgcg cgcgatatgc gtcagcgcct
    11641 gcgggacggt ccaggaatgg ccgctgtcgc cgatgtcgag cagatcgagc accgacaacg
    11701 gctcgtgcac gcgcaccagc gtctcctcgt cggcacggat ctcgccctgc accagcgcaa
    11761 gatgtgtttc gccggagagt ttctccagat aggcgatgag acggaagcgc ccgtaggcgg
    11821 tgtcgatttc gcgttcggtg acgcgccgca ccagcgactc atgcgcgctg cggaagttga
    11881 tcagatcggc gatcgagccg atcttgaggc cgtgcttccg ggcgaattcg aacagatcgg
    11941 gcaaccgcgc catggtgccg tcgtccttga ggatttcgca aatcactgcg gtggggctga
    12001 ggcccgccag ttgcgccagg tcgcaacccg cttcggtatg accggcgcgc accagcacgc
    12061 cgccgttctg cgccatgagc gggaagatgt gccccggctg gaccacgtcc tcgggtttgg
    12121 cgttcggcgc gaccgccgcc tgaacggtgc gggcgcgatc gtaggcggaa atgccggtgg
    12181 tgacgccggt ggccgcttcg atggagacgg tgaaggccgt gcccaggggc gcgcggttgt
    12241 cgcgcaccat gagcggcaga ttgagctgac ggcagcgctg ctcggtcagc gtgaggcaga
    12301 tcagcccgcg gccgtacttc gccatgaagt tgatcgcttc gggcgtgacg aaatcggcgg
    12361 cgagtacgag gtcgccctcg ttttcgcgat cctcgtcgtc gaccagcacg accatgcggc
    12421 cggctttgat ttcttcgatg atttccgcag tggtggcgaa ttgcatggcg cgacgcttga
    12481 aagagcaagc ccggattcta tcaggcgccg cggccgtccc cgggcgcggc cgcggcgacc
    12541 ctccggttag aatcgaccgc ccgacccgcg aaacgtgccc atgtcccgac gtgtcctgca
    12601 ctgccttggc tgcgccctgc tcgcggcaat gctgctcgtc gctccggccg gcgcagccga
    12661 tctgcgcatt gccatggcgg ccgacgtggg ttcgatggac ccgcatttcg ccaacatttc
    12721 gcccaacgtg gtcgtcagtt cgcatgtatt cgagacgctg accacggtgg acgccgacgc
    12781 gcggctgatg cccgcgctgg cggaatcctg ggcggcggtc ggtcccacca cctgggaatt
    12841 ccgcctgcgc gaggacgtgc gcttccatga cggcagtccc ttcaccgcgg aagatgtgct
    12901 gttctcgctg caacgccccg ccaccatcaa gaacagtccc ggcggcttca ccacgttcac
    12961 caaacccatc gtgcgcatgg aggcggtgga tcgccacacc gtgcgcctgc ataccgccgc
    13021 gccgtacgcg atggtgcctt acgatctggt ctcggtgttc atcgtttcca agcgcgcggc
    13081 cgagggggcg cgcagccgcg atttcgacga cggcacggcg atgatcggca ccggtcccta
    13141 tcgcttcgtg cgcttcgcgc gcggcgaagc catcgacctg gcgcgcaacg acgactactg
    13201 gggcaagcga ccggaatggg agcacgtgtc gctgcgcctc attcccagcg acccggcgcg
    13261 catcgcggcg ctgtacgccg gcgatgtgga catgatcgaa aacgtgccgc cggccgatgc
    13321 ggcgcgcctc gcgcgcgatc cgcgctttcg catcgaacgc aagatttcct ggcgcacgat
    13381 ctttctcacc ctggatcagt accgcgacga ctcgcctttc atcgatgcgg gcggtaagcc
    13441 aaaccccttg cgcgatgcgc gggtgcgacg ggcgctgtcc aaagccatcg atcgcgaagc
    13501 gatctgtgcc aaagtgatgg acggtttcgc cacgccgacg gcgaacatcg tcgcgccgac
    13561 cgtgttcggc catgcgcata gcctgcaggc cgaagcctac gatccggcgg gtgcaaaagc
    13621 actgctcgcc gaggcgggct acgccgacgg cttcgaactc acgctgcacg gccccaacaa
    13681 ccgctacatc aacgacgagt tgatcgtgca ggcggtggcg cagatgctgg cgcgcatcgg
    13741 tatccgcaca cgggtcgaaa ccatgccggt gaacatctat ttccccaagg cgcgcgcgga
    13801 aagcttcagc gtcgcgctgc tcggctgggg ctccttctcg ggcgatctgg cgttgcgatc
    13861 gctgttcatg acggagaacg ccacacaagg cacgggtgcc tggaactggg gccgctacag
    13921 caatccggag gtggatgagc tggtgcgccg cgcgctcggc gcgctcgagc cggccgagcg
    13981 cgaacgcctg acgcgcgagg ccacggtgcg cgcaatgggc gagacggcgc tgatcccgct
    14041 ctatcaccag atcgccagtt gggcgctgcg gcgcgacctg cgctacatcg cacgtaccga
    14101 cgaatacagc ttcgccttcg atgtgcacac ggagacgacg ccatgacggc ctatctcatc
    14161 cgccgtgcgc tgcaggcgct gctggtgctc ctggtcatgt cggcgctgct gttcgtcggc
    14221 atctacgcca tcggcaatcc ggtggaaatc ctgatcagcc cgcaggccga tcagcgcgac
    14281 atcgcgctcg ccacccaggc gctcggcctg gaccagccgc tctggcggca atacttcgcc
    14341 ttcgttgaac acgctctgcg cggcgatctc ggccactcct tcgtctacaa cgtgccggcc
    14401 ctcgagctgg tgctgtcgcg gctgcccgcg acgctggaac tcgccgtcgc ggcgatgctc
    14461 attgccatcg tgatcggttt gccgctgggc atctgggccg gcatgcggcc cgattcgtgg
    14521 ctgggcaaaa cgattatggc gggctcgata ctcggctttt cgctgccgac cttctgggtc
    14581 ggtctgctgc tcatcatgtt tttcggcgtg caactgggct ggttgccggc gagcggccgc
    14641 ggcacaacca cggattggtt cggcgtgccg gtgagcttca ccacgctcga cggcctggcg
    14701 catctggccc tgcccgcctt caatctggcg ctgttcaagc tgtcgctgct gatccgcctc
    14761 gcgcgcaacg gtgtgcgcga cgccttgcac caggactacg tgcgcacggc gcgcgccaag
    14821 ggtttgcacg aaggccaggt ggtgcggcgg catgtgctga agaacatcat gctgcccatc
    14881 gtcaccgtga tcgggctgga gttcggctcg ctggtcgctt tcgcggtggt gacggaaacg
    14941 atcttcgcgt ggccgggcgc cggcaagctc ctcatcgacg ccatcaatgt gctcgaccgg
    15001 ccggtggtcg tcgcatacat gctgctgacg gtgctgctgt tcgtgctgat caacttcgtg
    15061 gtcgatgtgc tctacaccgc gctcgatcca cgcgtgcgac tggcggggaa ccaatgaacg
    15121 cgctgcgacg cttcggggcc agccgccttg cgctcattgc cgcgacggtg ctcgcgctgc
    15181 tcgtattcgt cgccgtgttc gcaccggtga tcgcgccgca ggatccctac gatctggcgc
    15241 aactggatgt gatggaaggg cgtctgccgc ccggcgctgc gtcactcgat caaacgcggc
    15301 attactggct ggggaccgac gagcaggggc gcgacatgct gtcggcgatc ttctacggct
    15361 tacgcagcag tctgtacgtg ggcgtaacgg cgacgctgtg cgcactggcg ctggggtcaa
    15421 tcctcggtgt cgcggcgggc tatttcggcg gttggttcga gagcgtggc
//
LOCUS       DATGOU010000109         8201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_2113284, whole genome shotgun sequence.
ACCESSION   DATGOU010000109 DATGOU010000000
VERSION     DATGOU010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8201
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2113284"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /gene="guaA"
                     /locus_tag="VKZ48_06410"
     CDS             complement(<1..1081)
                     /gene="guaA"
                     /locus_tag="VKZ48_06410"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006402676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HLU77513.1"
                     /translation="MYDKILILDFGSQVSQLIARRVREAQVYCELHPYDVPADFISAY
                     APKGIILSGGPNSVYEADDWRVPQVVFELGVPVLGICYGMQAMAKQLGGEVEASNVRE
                     FGYAEIRARGHSKLFKDIQDRSNAEGHGLLDVWMSHGDKVTALPPGFKVIAGNASCPI
                     AGIADEARGFYGVQFHPEVTHTPKGREMIARFVRDICGCTADWTMPNYVDEAVARIRE
                     QVGGEEVILGLSGGVDSSVAAALIHRAIGDQLTCIFVDTGLLRKDEAKQVMETFGKHL
                     GVKVDMVDASERFMRELAGVTDPEQKRKIIGREFVHVFQEEAERLPNAKWLAQGTIYP
                     DVIESAGANTKKAHTIKSHHNVGGLP"
     gene            complement(1120..1476)
                     /locus_tag="VKZ48_06415"
     CDS             complement(1120..1476)
                     /locus_tag="VKZ48_06415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HLU77514.1"
                     /translation="MTVKDFRDLLVWRQAMDVAATVYRLTKTFPPEELFSLTAQLRRS
                     AISVASNIAEGQGRGSDAEFGRFLNIARGSLCELQTQLLLAQRVEARPVREFDNVLAE
                     VEELGRMLRGLQNTVG"
     gene            complement(1509..2969)
                     /gene="guaB"
                     /locus_tag="VKZ48_06420"
     CDS             complement(1509..2969)
                     /gene="guaB"
                     /locus_tag="VKZ48_06420"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020065652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HLU77515.1"
                     /translation="MPDIQKALTFDDVLLVPAHSSILPRDVSLRARVTRNITLNMPLL
                     SAAMDTVTEARLAIAMAQEGGIGIVHKNMPAKAQAAEVLRVKRFESGVVRDPITIPPD
                     MTVREVQALTQQHKFSGLPVVEGKKLVGIVTNRDLRFETRLDQPVRAIMTPRERLVTV
                     REGATLEEATVLLHKHRLERVLVVDERGDLTGLITVKDILKSSEYPLACKDEHGKLRV
                     GAAIGVGEGTEERAELLVEAGVDVIVVDTAHGHSQGVLERVKWVKRNFPHVEVIGGNI
                     ATAAAARALVDYGADAVKVGIGPGSICTTRVVAGVGVPQITAIQNVAKGLAGTGVPII
                     ADGGVRFSGDVPKAIAAGADCVMMGSLFAGTDESPGEIELFQGRSYKSYRGMGSLGAM
                     QQGAADRYFQDPAANADKLVPEGVEGRVPYKGSVVKVIEQLLGGLRASMGYLGCASID
                     EMHTRAEFVQITAAGMRESHVHDVQIVKEAPNYHID"
     gene            3126..3629
                     /locus_tag="VKZ48_06425"
     CDS             3126..3629
                     /locus_tag="VKZ48_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HLU77516.1"
                     /translation="MAVPLLRTALLAACLAVALPAGAQMYKWTDANGRVQYSDRPPSN
                     QQAQVIGRSGTPAPIAPAKEEARDADGKTAEDTTAPQTWAQKEQEFRKRRMEAEEERQ
                     KQAQLEAEERAKAEDCARTRNYLKAAEDGRRLSRTNDKGEIEYLDDAQIRAEAERTRQ
                     ELSRHCS"
     gene            complement(3719..4000)
                     /locus_tag="VKZ48_06430"
     CDS             complement(3719..4000)
                     /locus_tag="VKZ48_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HLU77517.1"
                     /translation="MPDIEVVYALPERQALYRVTVEEGATVRDAIEASGVLRDFPDID
                     LSRNRVGVYARLVALETPLRAGDRVEIYRPLQADPKTARRARAAAQRGK"
     gene            complement(4000..4440)
                     /locus_tag="VKZ48_06435"
     CDS             complement(4000..4440)
                     /locus_tag="VKZ48_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HLU77518.1"
                     /translation="MHTVERSALVLYPAEHMYSLVEDIESYPLFLPWCSGTEVHERDD
                     ARTVATIHVDYHGLRQQFTTENTKTPGERIDMRLVSGPFKSLDGLWTFKSLNGDSSKV
                     QLQLSYELANMLLERLGGPVFNYIANTFVDSFVRRADALHAAQS"
     gene            4531..4977
                     /gene="smpB"
                     /locus_tag="VKZ48_06440"
     CDS             4531..4977
                     /gene="smpB"
                     /locus_tag="VKZ48_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HLU77519.1"
                     /translation="MSIVDNRKAFHDYFIEERFEAGIVLEGWEVKAIRAGRVQLKEAY
                     IVIRNAELFLIGAHISPLGSASTHVNPDPVRTRKLLLHAEEIRKLIGKVERAGYTLVP
                     LNLHYKRGRIKLDMGLAKGKKQYDKRRAEKDREWQREKQRLLRANR"
     gene            complement(4984..7350)
                     /gene="ppsA"
                     /locus_tag="VKZ48_06445"
     CDS             complement(4984..7350)
                     /gene="ppsA"
                     /locus_tag="VKZ48_06445"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003811764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HLU77520.1"
                     /translation="MTDAYVVGFENLRMTDVERVGGKNASLGEMIGQLAQSGIRVPGG
                     FATTATAYRDFLRDAGLSERINARLKALDPEDVTALARCGAEIREWIIDAPLPARLEA
                     DITAAYQALMAEGEFPVAVRSSATAEDLPDASFAGQQETFLNVQGLPAVIQAIKHVYA
                     SLYNDRAISYRVHKGFDHESVALSAGVQRMVRSDLAASGVMFTLDTESGFPDVVFITG
                     AWGLGETVVQGAVNPDEFYVHKPMLEAGRPAILRRALGSKMVKMIFGERAEAERAVET
                     VDVPAADRHRFCVTDEEVLELARYAMTIERHYGRPMDIEWGKDGLDGRLYILQARPET
                     VKSQARHGVQERYRLKARSTVLVSGRAIGGKVGAGPVRLVSDATEMARVLPGDVLVTD
                     MTDPNWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERLHDGQQVTVS
                     CAEGDTGHVYEGILAVERTEQSLSDLPAIPVKISMNVGNPRLAFDLAGVPNAGVGLAR
                     LEFVINNMIAVHPKAALEYPNLPAELKRSVEEAARGYATPRAFFVEKLTEGVSTIAAA
                     FWPKPVIVRLSDFKSNEYRKLVGGSRYEPEEENPMLGFRGACRYLDPSFAECFAMECE
                     ALRKVRDTLGLTNVEIMVPFVRTLDEARGVIDLLARHGLRRGENGLRIVMMCELPSNA
                     ILAEQFLEYFDGFSIGSNDLTQLTLGVDRDSGLVAMAFNERDPAVLELIRMAIHACRK
                     QGKYVGICGQGPSDYPDLARWLAQEGITSLSLNPDTVVDTWLELAKAA"
     gene            7419..>8201
                     /locus_tag="VKZ48_06450"
     CDS             7419..>8201
                     /locus_tag="VKZ48_06450"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HLU77521.1"
                     /translation="MPARTVFFVSDGTGITAEALGRSLLTQFEGMQFVQQVLPFVDTV
                     EKAEECLQRFEAARKASAARPIVFTTLLDEELRAIIRRADALVFDFFENFLAPLEAEL
                     GVRSSHRVGRSHGMADDPEYQARIEAVNFALSYDDGQNPRGLPEADVILVGVSRCGKT
                     PTCLYMALQLGIKAANYPLVPEDFERGALPAALEPHLDRLYGLTIAPARLTVIRQERR
                     PGSTYADPENCRYEVTEAERLMRRYGLRWLDSTARSIEEIAIH"
BASE COUNT         1522 a         2718 c         2493 g         1468 t
ORIGIN      
        1 cgggcaggcc gccgacattg tggtgcgatt tgatcgtatg cgccttcttc gtgttcgcgc
       61 ccgccgattc gatcacgtcc ggatagatgg tgccctgcgc aagccacttg gcgttcggca
      121 ggcgctccgc ctcttcctgg aagacatgca cgaactcgcg gccgatgatc ttgcgcttct
      181 gttccggatc ggtcacgcct gccagttcgc gcatgaaccg ctcggaagcg tcgaccatgt
      241 ccactttcac gccgaggtgc ttgccgaagg tctccatcac ctgcttcgct tcgtctttgc
      301 gcagcaggcc ggtgtccacg aagatgcagg tgagctgatc gccgatggcg cgatggatca
      361 gcgccgcggc gacggaagaa tcgacgccgc ccgacaggcc gagaatcacc tcttcaccgc
      421 ctacctgttc gcgaatgcgc gcgactgcct cgtcgacata gttgggcatc gtccaatccg
      481 ccgtgcagcc gcagatgtcg cgcacgaaac gcgcgatcat ttcgcgccct ttgggcgtgt
      541 gcgtcacttc ggggtggaac tgcacgccgt aaaaaccgcg cgcttcgtcc gcgatgccgg
      601 cgatcgggca ggaggcgttg ccggcgatga ctttgaagcc cggcggcagt gcggtgacct
      661 tgtcgccgtg actcatccat acatcgagca gtccgtggcc ttcggcgttg ctgcgatcct
      721 ggatgtcctt gaagagcttg gagtgtccgc gcgcccggat ttcggcatag ccgaattcgc
      781 gcacgttcga cgcctcgacc tccccgccca actgcttggc catggcctgc atgccgtagc
      841 agatgcccag cacgggcacg cccagctcga ataccacctg cggtacgcgc cagtcatcgg
      901 cttcgtagac cgaattcggg ccgcccgaca ggatgatgcc cttcggcgca taggcactga
      961 tgaaatccgc gggcacgtcg tagggatgga gttcgcagta cacctgcgct tcgcgcacgc
     1021 gccgcgcgat gagttgcgag acctgcgagc cgaaatcgag aatcaggatc ttgtcgtaca
     1081 tgaggaaata gccgagagcc gagagccgag agtgaaagtt cagccgactg tgttctgtaa
     1141 gccgcgaagc atacggccta attcttctac ttctgccagc acgttatcga attctcttac
     1201 tggccgagcc tccacccgct gtgcaagcag caattgcgtc tgcaactcgc acaacgatcc
     1261 tctcgcaatg ttcaaaaagc gcccgaactc cgcgtccgaa cctcgcccct gaccctcagc
     1321 gatatttgac gctacggata tcgcagaccg acgcaactgc gcggtcaagc taaacaactc
     1381 ctcaggtgga aaggtcttgg tgagccgata aacggtagcc gcgacgtcca ttgcttgccg
     1441 ccaaaccagc aaatcacgaa agtccttgac cgtcatgcgc tctcggctct cggctcttcg
     1501 ctctaggctc aatcgatgtg atagttgggc gcttccttga cgatctgcac atcgtgcaca
     1561 tgcgattcgc gcatgccggc ggccgtgatc tgcacgaact cggcgcgggt gtgcatttcg
     1621 tcgatgcttg cgcagcccag gtaacccatg ctggcgcgca ggccgccgag cagttgttcg
     1681 atgactttca ccaccgaacc cttgtacggc acgcggcctt ccacgccttc gggcacaagt
     1741 ttgtcggcgt tggcggccgg gtcctggaaa taacggtctg cggcgccctg ctgcatcgcg
     1801 cccagcgaac ccatgccgcg ataggacttg taggagcgcc cctggaacaa ttcgatttcg
     1861 cccggcgact cgtcggtgcc cgcaaacagg ctgcccatca tgacgcagtc cgcgccggcg
     1921 gcgatggctt tgggcacgtc gccggaaaaa cgcacgccgc cgtccgcaat gatcgggacg
     1981 ccggtgccgg ccaatccctt cgctacattc tggatagcgg tgatctgcgg cacgccgacg
     2041 cctgcgacca cgcgggtcgt gcagatcgaa ccggggccga taccgacctt gaccgcatcg
     2101 gcgccgtaat cgaccagcgc gcgcgccgcc gcggcggtcg cgatattgcc gccgatgacc
     2161 tcgacgtgcg ggaagttgcg cttgacccac ttgacgcgct cgagcacgcc ctgcgaatgg
     2221 ccgtgcgcgg tgtcgaccac gatcacgtcc acgccggctt cgaccagcaa ttcggcgcgc
     2281 tcttccgtgc cttcgccgac gccgatggcc gcgcccacgc gcagcttgcc gtgttcgtcc
     2341 ttgcacgcga gcggatattc gctcgacttg agaatgtctt tgacggtaat gagaccggtc
     2401 agatcacccc gttcgtccac caccagcacg cgctcgaggc gatgcttgtg caggagcacg
     2461 gtcgcttcct cgagcgtggc accttcgcgc accgtcacca gccgctcgcg cggcgtcatg
     2521 atggcgcgca ccggctgatc gaggcgggtt tcgaagcgca aatcccggtt ggtgacgatg
     2581 ccgaccagtt tcttgccttc aaccacgggc aggccggaga acttgtgctg ctgcgtgagc
     2641 gcctggactt cgcgcaccgt catgtccggc ggaatggtga tcggatcacg caccacgccc
     2701 gattcgaagc gcttgacgcg cagcacttcg gccgcttgcg ccttggcggg catgttcttg
     2761 tgcacaatgc cgatgccgcc ttcctgcgcc atggcaatgg ccagtcgggc ttccgtcacc
     2821 gtgtccatgg cggcggacag caacggcatg ttgagcgtga tgttgcgcgt aacgcgggca
     2881 cgcaggctca catcacgcgg cagaatgctg gaatgggcgg ggacgagcag gacgtcgtcg
     2941 aacgtcaggg ccttttgaat gtcgggcatg gtgactctgg cgaccaaagc cgcattatac
     3001 gtggcttgaa gatgattagg aacgtgcacg gcgcgtgccg aatgcacatt tgcattccgg
     3061 cgcgcattga cgggccgacg gggcgccgct atgttgcagc cagcccccca tatcaggaca
     3121 atgtcatggc tgttccgctc ctccgcacgg cgctgctggc tgcctgcctg gccgtcgccc
     3181 ttcccgccgg cgcacagatg tacaaatgga ccgacgccaa cggccgcgtg cagtactccg
     3241 accgaccgcc ttccaatcag caggcccagg tgatcggccg ttccggcacc cccgcaccta
     3301 ttgcgcctgc caaggaagaa gcgagggatg cggacggcaa aaccgccgag gacaccactg
     3361 cgccgcagac ctgggcccag aaggagcagg aattccgcaa gcgccgcatg gaagccgaag
     3421 aggaacggca gaaacaggcc caactggagg ccgaggagcg agcgaaggcc gaggattgtg
     3481 cacgtacgcg taattacctc aaggcagccg aagacggccg gcgcctctcc cgcaccaacg
     3541 acaagggcga aatcgaatac ctggacgacg cgcagatacg cgccgaagcc gaacgcaccc
     3601 ggcaggaatt gagccggcac tgcagttagg aaaacgctga ccgtgcttcg gacggtacgc
     3661 aacagcgctg gctaaaccac cacggtcgtg gcccccgccg gacggcccga tgccgagact
     3721 atttgccgcg ttgcgccgcc gcgcgcgcac ggcgcgccgt tttcgggtcg gcctgcagcg
     3781 gccgatagat ttcaacccga tcgcccgcac gcagcggtgt ctcgagcgcc acgagccggg
     3841 cgtacacgcc gacccggttg cgcgacagat cgatgtcggg aaaatcacgc agcacgccgc
     3901 tggcctcgat cgcgtcccgc actgtcgccc cctcctcgac cgtgacccgg taaagcgcct
     3961 gccgctcggg cagcgcatag accacctcga tgtccggcat cagctttggg ccgcatgcaa
     4021 cgcgtcggcg cgccgcacga aggaatcgac gaacgtgttg gcgatgtaat tgaacaccgg
     4081 gccgcccagc cgttcgagca gcatgttggc cagctcgtag ctcagttgca gttgcacctt
     4141 gctgctgtcg ccgttcagcg atttgaaggt ccacaggccg tccagcgatt tgaacggtcc
     4201 cgacaccagg cgcatgtcga tgcgctcgcc cggcgttttg gtgttctcgg tggtgaactg
     4261 ctggcgcagg ccgtgataat cgacatggat cgtggcgacg gtgcgcgcgt cgtcgcgctc
     4321 atgaacctcg gtgccgctgc accaggggag aaacagcgga taggactcga tgtcctcgac
     4381 caggctgtac atgtgctcgg cgggataaag cacgagggcg gaacgttcga cggtgtgcat
     4441 gaacgggact gttagaatcg cagccggcat tttaatgagc gacgcgccca gcgtcgatgg
     4501 aactcaggcg cgacacccga tcggacctca atgagcatcg ttgacaatcg caaggctttc
     4561 cacgactact tcatcgaaga gcgcttcgag gccggcatcg tgctcgaagg ctgggaagtg
     4621 aaagccatcc gcgccggccg ggtgcagttg aaggaagcct acatcgtcat ccgcaatgcc
     4681 gaactgttcc tcatcggcgc gcatatcagt ccgctcggct ccgcctcgac gcacgtcaat
     4741 ccggatccgg tgcgcacccg caagctgctc ctgcatgccg aggaaatccg caagctgatc
     4801 ggcaaggtcg agcgcgccgg ctacacgctg gtgccgctga acctgcatta caagcgcgga
     4861 cgcatcaagc tcgacatggg cctcgcgaag ggcaagaagc aatacgacaa gcgccgggcg
     4921 gaaaaagatc gcgaatggca gcgcgaaaaa caacggctcc tgcgtgccaa tcgctagcga
     4981 tcttcatgcc gccttcgcca gctcgagcca ggtatcgact accgtatccg gattgagcga
     5041 caggctggtg atgccttcct gcgccagcca gcgcgccaga tccggatagt ccgaaggccc
     5101 ctgcccgcag atgccgacgt acttgccctg cttacggcag gcatgaatgg ccatgcggat
     5161 caattccaac accgccggat cacgctcgtt gaaggccatg gccaccagcc cggagtctcg
     5221 atcgacgccc agcgtcaatt gcgtcaaatc gttggaaccg atggagaagc cgtcgaagta
     5281 ttcgagaaac tgctccgcga ggatcgcatt cgacggcaac tcgcacatca tcacgatacg
     5341 cagcccgttc tcgcctcggc gcaagccgtg acgggcgagc aggtcgatca cgccgcgcgc
     5401 ctcgtcgagc gtgcggacaa agggcaccat gatctcgacg ttggtcagac ccagcgtgtc
     5461 gcgcactttg cgcagcgcct cgcactccat ggcgaagcac tcggcgaagg acggatcgag
     5521 ataacggcag gcgccacgga acccgagcat cgggttttcc tcctccggct cgtagcgact
     5581 gccgccgacg agcttgcggt actcgttcga tttgaaatcc gacagacgca cgatgaccgg
     5641 tttcggccag aacgcagcgg cgatggtgct gaccccttcg gtcagctttt cgacgaagaa
     5701 ggcgcgcggc gtggcgtagc cgcgagcagc ctcttccacc gagcgtttca attccgcagg
     5761 cagattcgga tattccagcg cggccttggg atgcacagcg atcatgttgt tgatcacgaa
     5821 ttccagacgc gccaatccca cgccggcatt gggcacgccg gccagatcga aagccagtcg
     5881 cggattgccg acgttcatgc tgatcttgac cggaatggcc ggcagatcgc tcaacgactg
     5941 ctcggtccgc tcgacggcca ggatgccctc gtacacatgc ccggtatcgc cttcggcgca
     6001 cgacaccgtc acctgctggc cgtcgtgtaa tcgctcggtg gcgtcaccgc accccacgac
     6061 cgccggaatg ccgagttcgc gtgcaatgat ggcggcgtga caggtccggc cgccgcgatt
     6121 ggtcacgatc gccgatgcgc gcttcatcac cggctcccag ttcggatcgg tcatgtcggt
     6181 gacgagcaca tcgccgggca gcacacgcgc catttccgtc gcgtcgctca ccagccgcac
     6241 cggtcctgcg ccgaccttgc cgccgatcgc ccggccgctc accagcacgg tggaacgtgc
     6301 cttgaggcga taacgctcct gcacgccatg gcgcgcctgc gatttcaccg tctccggccg
     6361 ggcctgcagg atgtacaggc ggccatcgag cccgtccttg ccccattcga tgtccatggg
     6421 ccggccgtaa tgccgttcga tggtcatcgc ataacgcgcg agttccagca cctcttcatc
     6481 ggtgacgcag aaacgatgcc gatccgccgc gggcacgtcg accgtttcga ccgcgcgctc
     6541 cgcctccgcc cgctcgccga agatcatttt gaccatcttg gatcccaggg cgcggcgcag
     6601 gatggcgggc cgacccgctt ccagcatcgg cttgtgcaca tagaactcgt cgggattgac
     6661 cgcgccctgt acgaccgttt cgcccagtcc ccatgcgccg gtgatgaaca ccacgtcggg
     6721 gaagcccgac tcggtatcca gcgtgaacat cacgccgctt gccgccagat cgctgcgcac
     6781 catgcgctgc acgccggccg acagcgctac cgactcgtgg tcgaagccct tgtggacgcg
     6841 ataggaaatg gcgcgatcgt tgtagagcga ggcgtaaaca tgcttgatcg cctggatgac
     6901 tgccggcaaa ccctgcacat tgagaaaggt ctcctgctgg ccggcgaagg aagcgtccgg
     6961 caagtcctcg gcggtggcgc tcgaacgcac ggccacggga aactctccct cagccatcaa
     7021 tgcctgatat gccgccgtga tgtccgcttc cagacgggcg ggcagcggcg catcgatgat
     7081 ccattcccgg atttccgcgc cgcaacgcgc cagcgcggtg acgtcctcgg gatcgagtgc
     7141 cttgaggcgc gcgttgattc gttcgctgag accggcgtcg cgcaggaaat cgcgataggc
     7201 cgttgccgtg gtggcaaagc cgcccggcac gcgtattccc gattgagcaa gctggccgat
     7261 catttcgcca agcgaggcgt ttttgccgcc cacccgctcg acgtccgtca ttcgcaggtt
     7321 ttcgaaaccc accacataag cgtccgtcat gatgcctccg gttgccgcgt gccgaagaag
     7381 tggctatttt agccgggccc atccctcaat cacgaaacat gcctgcgcgc accgtttttt
     7441 tcgtttcgga cggcaccggc atcactgccg aagcgctggg gcgcagtctg ttgacacagt
     7501 tcgagggcat gcagttcgtg cagcaggtgc tccccttcgt cgacactgta gagaaggcgg
     7561 aagaatgcct gcaacgcttc gaggcggcac gaaaggcatc cgccgcacgt cccatcgtct
     7621 tcaccaccct gctcgacgaa gaattgcgcg ccatcatccg ccgggccgac gcactggttt
     7681 tcgatttttt cgagaatttt ctcgcgccgc tggaggcgga actcggcgtg cgctcgtcgc
     7741 atcgggtcgg ccgctcacac ggcatggccg acgaccccga ataccaggca cgcatcgagg
     7801 cggtgaactt cgccctctcc tatgacgacg gccagaatcc gcgggggctg cccgaggcgg
     7861 atgtcatcct ggtcggcgtc tcccgctgcg gcaagacgcc gacctgcctt tacatggcac
     7921 tgcaacttgg catcaaggcg gcgaactatc cactggtacc ggaggatttc gagcggggcg
     7981 cactcccggc cgcactcgag ccgcatctgg accggctgta cggcctgacc atcgcgccgg
     8041 cccgtcttac cgtcattcgc caggagcgcc ggcccggcag cacttacgcc gatccggaaa
     8101 actgccgcta tgaagtgacg gaagcggagc gtctgatgcg ccgctacggc ctgcgctggc
     8161 tcgattcgac cgcgcgctcc atcgaggaaa tcgccatcca c
//
LOCUS       DATGOU010000110        16711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_4309896, whole genome shotgun sequence.
ACCESSION   DATGOU010000110 DATGOU010000000
VERSION     DATGOU010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 16711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16711
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4309896"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..996)
                     /locus_tag="VKZ48_06455"
     CDS             complement(<1..996)
                     /locus_tag="VKZ48_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HLU77522.1"
                     /translation="MERQEQLSQRDTSRRDEFTASGIGEEVLRRLDAYWRASNYLSVG
                     QIYLYDNPLLREPLSREHIKPRLLGHWGTTPGLNFIYTHLNRVIREQQVPMLFVTGPG
                     HGGPGPLSQTYLEGSYSEFYPEVSQDEEGMRLLFKQFSFPGGMPSHTAAEVPGSIHEG
                     GELGYSLTHAYGAAFDNPELLVACVIGDGEAETGPLAASWHSNKFLNAAHDGAVLPIL
                     HLNGYKIANPCVLARIPREELEALLVGYGHRPYFVDVEADDHMAAHRAMADTLDRVVA
                     EIREIQRQAREAVAAGRTFERPRWPMIVLVSPKGWTGPKRVNDQQIEGTFRSHQVP"
     gene            complement(1006..1347)
                     /locus_tag="VKZ48_06460"
     CDS             complement(1006..1347)
                     /locus_tag="VKZ48_06460"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HLU77523.1"
                     /translation="MADSNRTSNDHQNTAQSPVPAQWGLRQPRESHSADPIFVPQPPR
                     EHEIRWPGNADDLSPAEISRLISATAYSLAARRGYHDGYDIEDWLGAEAEVNARLEEI
                     RRAARVGTPAG"
     gene            complement(1354..2331)
                     /locus_tag="VKZ48_06465"
     CDS             complement(1354..2331)
                     /locus_tag="VKZ48_06465"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HLU77524.1"
                     /translation="MSDIVLAADIGGTKSYLALAEPVGGGLRALCVERFRNDDYVDFN
                     AILRRFVKEQAAGRVIRRAAVGVAGPRDGDRVWLTNRPWLVDAVALSDILGGAPVRLL
                     NDLEATAYGIDSLAADEVLTLQHGERIDHAPQVIVAAGTGLGIAYRVWDGLRYRVIGS
                     EGGHMGFAPLDLRQLDLCRALYERLGRVEAEHVVSGAGIARIYSFLGGPSGTSPPQVS
                     MRAFQEQDPLALEALEIFSACYGAVAGDHALAMLAYGGVYLAGGIAPKVLQPQQASHL
                     LTAFNSKSGHSGVVERVPVHVVLNDQIALFGAVLAALQDMAAMPPTTKE"
     gene            2409..3878
                     /gene="glgA"
                     /locus_tag="VKZ48_06470"
     CDS             2409..3878
                     /gene="glgA"
                     /locus_tag="VKZ48_06470"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HLU77525.1"
                     /translation="MNGATPRILFVTPECAPWVKTGGLGDVSAALPAALQALGCDVAI
                     LMPAYRGMHAIAQRAQEAATIPALAELPAARILEAHLDNGVTMLLLDAPALYDREAGP
                     YLDATGRDWPDNHLRFGALSHAAAVVARNGLAGGWRPDLVHCNDWPAALAPAYLRFEP
                     PTEPVPTLLTIHNLAFQGLFPAQTLAQLGLPKHSYDIDGLEFWGQVSFLKGGICYADA
                     ITAVSPNYAREIQGKELGFGLDELLHARRDVLHGIVNGIDTDYWNPATDPMIAAQYDV
                     GWFDAKPFNKLALQKRLSLPQDSRIPVLGTVGRITLQKGFDLIAAIAEELMEIPVHLV
                     MLGSGSLEIEEQLLRLQSAYPKCIGLKIGFDEKLAHMIEAGADMFLMPSRFEPCGLNQ
                     MYSQRYGTPPVANATGGLVDTIIDYDGTPESAERATGFLFHEASPEALLEAIRRALDC
                     YREPELWRKLCFNGMKTDFSWQASAKRYVDLYRSQIKKT"
     gene            complement(4071..6623)
                     /gene="glgP"
                     /locus_tag="VKZ48_06475"
     CDS             complement(4071..6623)
                     /gene="glgP"
                     /locus_tag="VKZ48_06475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HLU77526.1"
                     /translation="MVSPTEYSLDVNPNIPHRLARLEELANNLWYSWDRATRSLFARL
                     HPPLWDAVGHSPKAMLKRVDEQRLFEAADDPAYLSAFNRVLSAYDSYHSEPLRRNGSE
                     WLRQTDLVAYFCAEFGFHESFPIYSGGLGILAGDHCKAASDIRLPFVGVGLLYRQGYF
                     SQTIDREGNQQPHYQDSDFDDLPVTPVLQDNGEPLRVQVELPGRVIDVQVWQARIGHV
                     TLYLLDTDLESNSESDRDIAHHLYGGDRTTRIEQEIVLGVGGVRALGALGLKPTVWHI
                     NEGHAAFLVLERTRAHVKQGLDFATALEAVAVNTVFTTHTPVAAGHDHFAEEMIVEYF
                     KAYCGDLDLEPERLLALGRTPFNTDFNMTALAVRGSRHHNGVSRIHGRVSAEMLKDLW
                     PQVPANENPVTYVTNGVHTPTFVAPEWNAIFERFMAFDWGTRHSDEEMWARLMAMPDH
                     IYWSTRQHLKAQMLHLVRHRVRIQHYRNQGSEAHLDRLLRFVDPESPNVLTVGFGRRF
                     ATYKRATLLFQDLELLHKIVSDPERPVVFIFAGKAHPADVPGQDLIRTVSRVSRMPEF
                     EGHILLLEGYDMRLGRRLTTGVDVWLNNPVYPLEASGTSGMKAGMNGVINLSVLDGWW
                     GEGYCGDNGWAIKPATEASSEQQRDREEAHTLYEILQDNVVPLYYNRGNMGYSPDWVR
                     MSKRSIATILPRFNARRMINEYVEKFYLPASQSGRKYAEGGYANARLIAQWKAQVRAA
                     WPNVAMRRLDTPKRRIRFGESMRIEVAVDLDTLSPADVSIEAVVTRGLRETEERRVYA
                     FAADGTRTEAGEYRFVLDLAPELCGRLDYRIRAYPAHDLLTHPFELGLMIWA"
     gene            6722..8362
                     /locus_tag="VKZ48_06480"
     CDS             6722..8362
                     /locus_tag="VKZ48_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015003980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLU77527.1"
                     /translation="MESSRMSSGNVVSHNLPPPLADFDAWQSDALLRAAVAREGADWI
                     EARAVPLGRLVGSVAFQEWATQANTHPPVLQAYDRNGDRIDVVDYHPAYHQLMQAAFG
                     AGLHSLAWKETREGRYVARAALNYLWNQGEQGTACPVTMTFASVQVLRNAPDLAAQWE
                     AKILADDYDPRSLPLAHKRAATIGMAMTEKQGGSDLRSNAAVAIPVSGDEHVLLGHKW
                     FCSAPMSDGFLTLAQRSEGPTCLFVPRILPDGTRNSLRIVRLKDKLGNRSNASAEIEY
                     ERTHAWLVGEPGRGIGTLIEMAHLTRFDIVIAVAGMMRAALRQVLHHTRHRAAFGAPL
                     IEQPLMRNVLADLALETEAAMLLAFRLAGAFDRAAQDERARIYARVVTPAAKYWLCKR
                     LPMLAAEALECLGGNGYVEDWPMARFYREAPLNGVWEGSGNVICLDVLRALAKTPGAL
                     DMLDVELAQAARHPAIARHLGLLHRAVHEEPAGGEARRLTEGLALCLQAALLLEHAPP
                     AVAEAFIAARLGGQGGLAFGTLPAETDCAAIIARHREA"
     gene            8415..9359
                     /locus_tag="VKZ48_06485"
     CDS             8415..9359
                     /locus_tag="VKZ48_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019939137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HLU77528.1"
                     /translation="MTLTELRYVVAVARERHFGRAAEACFVSQPTLSVAVRKLEDELG
                     VTLFERGSGEVAITPIGERVIEQAQRVLEQAAMVKEVAKQGKDQLNGALRMGVIFTIG
                     PYLLPELVPLLKKRAPRMPLMLQEQYTHRLIELLRQGEIDVAILALPLEANGLMVQPL
                     YDEPFVVAVPRAHPWAARKTVSSRELKDEHMLLLGTGHCLRDQVLEVCPELNRFSATA
                     DGIQKSFEGSSLETIRHMVASGTGVTVLPATAVPRGAKRDRLLAYIPFSKPVPDRRVV
                     LAYRKTFPRPEAIEVVREAVLSCDLPCVEMLDLAPEPS"
     gene            9480..9977
                     /locus_tag="VKZ48_06490"
     CDS             9480..9977
                     /locus_tag="VKZ48_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008351500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HLU77529.1"
                     /translation="MAKNTRGYAANINIGLSDKERMKIAEGLSRLLADTYTLYLKTHN
                     FHWNVTGPMFNTLHLMFEEQYTELATAVDEIAERIRALGVPAPGTYKDYARLSSVKET
                     EGVPSANEMVQDLVEGQEAVTRTAREVFEVAEKANDQPTADLLTQRMQVHEKTAWMLR
                     TLLEK"
     gene            complement(10221..12053)
                     /locus_tag="VKZ48_06495"
     CDS             complement(10221..12053)
                     /locus_tag="VKZ48_06495"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLU77530.1"
                     /translation="MSPRRAPTPIALAVGGILVSGTLAYAAGDRGAIGAASFDPLLDD
                     PVLCLSGSSRPQALRYGARLAQLAQSPRSPELRPVDRPQMAPTPDDESVDPPLWAGLG
                     ALHYKITTRSPAAQQYFDQGLRLSHAFNHGEARRAFRAAQKRDPDCAMCYWGEALVLG
                     PNINAPMSPDAVAPAMAALTKAQQLAPQASVRERALIEALATRYTAQPPTDRAALDAA
                     YAEAMASAHRRFPQDTIIATLYAEAMMDTQPWDYWQPGGAQPKGHGAEIVQVLERVLK
                     AHPDHPGAIHLYIHAVEASNRPGRAESHAERLEKLMPGAGHIVHMPSHIYYRVGRYID
                     SLAANKRAVATDEAYLSKSKAEGIYAWGYYPHNVHFLMASAQMAGDGPTAVAAAEKLS
                     AVIPDEALRDVPLVHPVKVAAYFAHAQFSDPATVLALPDPGDDYPYIKAIWHYARGTA
                     HAAAGDIPAARAEADAIARLNRESDFKALEEALIPVPQVLRIAEQTVRARAAQAAGDL
                     PQATRHFRAAIAAQEHLAYMEPPYWYYPLHQSLGAVLLAQGDLDGAEQAFRTSLVRAP
                     NNGWALYGLTQVLEKRGDRKQAAAMQQRFQKAWAGKAEVDLARL"
     gene            12299..12532
                     /locus_tag="VKZ48_06500"
     CDS             12299..12532
                     /locus_tag="VKZ48_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77531.1"
                     /translation="MRSLIGVFLLALLLAGCEQQPSEQTMEDARESSAEMREDARDAA
                     AEVREAAEDAADATREGAQELKEEAREARKDAK"
     gene            13045..14967
                     /locus_tag="VKZ48_06505"
     CDS             13045..14967
                     /locus_tag="VKZ48_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020165153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrkA family serine protein kinase"
                     /protein_id="HLU77532.1"
                     /translation="MGVIESYVSNYARTREAEMTLAEYLDLCKRDPMAYATAAERMLA
                     AIGEPEVIDTRNDPRLSRIFSNRVIRRYPAFKEFYGLEDVVAQIVAFFRHAAQGLEER
                     KQVLYLLGPVGGGKSSLAERLKALMEVNPIYALKGSPVNESPLGLFNPERDADTLEAE
                     YGIPRRYLHGIMSPWAVKRLEEYQGDISQFKVVRLMPSVLRQVAISKTEPGDENNQDI
                     SSLVGKVDIRKLEHYAQDDPDAYSFSGGLCLANQGLLEFVEMFKAPIKMLHPLLTATQ
                     EGNFKGTEGFGAIPFQGIILAHSNESEWQSFRNNKHNEAFLDRVYIVKVPYCLQVTEE
                     VQIYQKLLRSSSLASAPCAPHTLEMMAQFSVLTRLKEPENSNIYSKMRIYDGENLKDI
                     DPNAKTFQEYRDYAGIDEGMTGMSTRFAYKVLSSVFNYDQTEIAANPVHLMYVLEQRI
                     MREDLPEETKRQYLAFIKEHLAPRYAEFIGKEIQTAYLESYSEYGQNIFDRYVTFADY
                     WIQDEDYRDPETGESFDRASLNAELEKIEKPAGIANPKDFRNEIVNFVLRARAQGGGK
                     NPAWTSYEKLREVIERKMFSNTEELLPVISFNAKASAEDRSKHENFVARMVQKGYTEK
                     QVRLLCEWYLRVRKSA"
     gene            15104..16288
                     /locus_tag="VKZ48_06510"
     CDS             15104..16288
                     /locus_tag="VKZ48_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019938179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HLU77533.1"
                     /translation="MSTFIDRRLNDRRKSAVNRQRFIERYKDHLRRAVSDIVAKRSIK
                     ELESGGQVQIPVKDITEPAFRHGPGGDREIVHPGNRTFAPGDRLPRPDDGEGDGQGGA
                     AGEGEMLDSFAFTLSREEFLQLFLDDLELPRLARTVLGSIKDKRSQPAGFTRTGSPRN
                     LHIVRSLKQSLARRMAARGTVGALERPVGGGRRVPFLDEVDLRYRHRVTVPKPVSQAV
                     MFCLMDVSGSMTEDKKDLAKRFFALLYLFLTRKYEKVDLVFVRHTDDASEVNEEEFFH
                     GNYSGGTVVLSALELMKEIVDARYPPALWNIYGAQASDGDAFGADPEKSRGFLEKELL
                     PASRYFAYIDIPTEGLEQTSTLWQAYQRIDQPHFAMKRVANRTDIYPVFRELFRKETV
                     KS"
     gene            16312..>16711
                     /locus_tag="VKZ48_06515"
     CDS             16312..>16711
                     /locus_tag="VKZ48_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HLU77534.1"
                     /translation="MKKPISTGCDWNFELLQEYDEAIADIAINEYKLDVFPNQIEVIS
                     SEQMLDAYASTGMPIGYHHWSFGKEYLRHEQAYRKGMRGLAYELVINSNPCIAYLMED
                     NTMTLQALVIAHACYGHNSFFKGNYLFRSWT"
BASE COUNT         3142 a         5390 c         5261 g         2918 t
ORIGIN      
        1 cgggacctgg tgcgaacgga aggtgccttc gatctgctga tcgttcacgc gctttggtcc
       61 ggtccagccc ttgggcgaaa ccagtacgat catcggccag cgcggccgct cgaaagtccg
      121 gccggcggcg accgcctcgc gcgcctgccg ctgaatctcg cgaatctcgg ccacgacccg
      181 gtcgagcgtg tcggccatgg cgcgatgcgc cgccatgtgg tcgtccgcct cgacatcgac
      241 gaaataggga cgatggccgt aaccgaccag cagcgcctcc agttcctcgc gcggaatgcg
      301 cgccaggacg cacggattgg cgatcttgta gccgttcagg tgcaggatcg gcagcaccgc
      361 gccgtcatgc gcggcattga gaaacttgtt ggaatgccag cttgccgcaa gcggtccggt
      421 ttccgcctcg ccgtcgccga tgacgcaggc caccagcagt tccggattgt cgaacgccgc
      481 accgtaggca tgcgtcagcg agtaaccgag ttcgccgcct tcatggatcg aacccggcac
      541 ttccgccgcg gtatgactgg gcatgccgcc gggaaaggag aactgcttga acagcaggcg
      601 catgccctct tcgtcctgcg acacctccgg atagaactcg ctatagctgc cttccagata
      661 ggtctgcgag aggggcccgg ggccgccgtg ccccggaccg gtgacgaaca gcatcggcac
      721 ctgctgttcg cggatgacac gattgaggtg cgtgtagatg aagttcagcc ccggcgtggt
      781 gccccagtgg ccgagcaggc gcggcttgat gtgctcgcga ctgagcggtt cgcgcaacaa
      841 cggattgtcg taaagataga tctgcccgac cgagaggtag ttggacgccc gccagtaggc
      901 gtcgaggcgc cgcagcacct cctcgccgat gcccgaggcg gtgaactcgt cgcgccggga
      961 cgtgtcgcgc tgcgaaagct gttcctgtcg ttccatagtc ccggctcaac cggccggcgt
     1021 gccgacacgc gccgcgcgac ggatttcctc caggcgcgca ttgacctcgg cttcggcacc
     1081 gagccagtcc tcgatgtcgt agccgtcgtg gtagccgcgc cgcgctgcga ggctataggc
     1141 ggtcgccgaa atcagccggc tgatttccgc cggcgagaga tcgtccgcgt tgccgggcca
     1201 gcggatctcg tgctcgcgtg gcggttgcgg cacgaaaatc gggtcggccg aatgcgactc
     1261 gcgcggctgc ctcaggcccc actgcgccgg caccggcgac tgggcggtgt tctgatggtc
     1321 gttactggtt ctgttcgaat cggccatttt tcttcactcc ttcgtcgtcg gcggcatcgc
     1381 cgccatgtct tgcagcgcgg caagcactgc tccaaacaat gcgatctggt cgttcaaaac
     1441 cacgtgcacg ggtacgcgct ccacgacacc gctgtggccg ctcttgctgt tgaaagccgt
     1501 aagcaagtga ctggcctgtt gcggctgcag taccttggga gcgataccgc cggccagata
     1561 aacgccgccg taagcgagca tggccagcgc gtggtcgccc gcgacggcgc cgtagcaggc
     1621 gctgaagatt tccagcgctt ccaaggcaag ggggtcctgc tcctgaaagg cgcgcatgga
     1681 cacctgcggc ggactcgtac cgcttggacc accgagaaag gaataaattc tcgcgatgcc
     1741 ggcgccggag accacatgct cggcctccac ccggcccagc cgctcgtaca gtgcgcggca
     1801 caggtcgagt tgccgcagat cgagcggcgc gaagcccatg tgaccgcctt cgctgccgat
     1861 gacgcgataa cgcaagccgt cccagacgcg ataggcgatg cccaggccgg tgccggcagc
     1921 gacgataacc tgcggtgcgt gatcgatccg ttcaccatgc tgcaacgtca gcacttcgtc
     1981 ggcggcaagg gagtcgatac cgtaggcggt tgcttcaagg tcgttgagca aacgcacggg
     2041 tgcgccgcca agaatgtcgg agagggcgac ggcgtcgacc agccacggcc ggttggtgag
     2101 ccatacacga tcgccgtcgc gcggccctgc cacgcccacc gctgcgcggc gaatgacgcg
     2161 gcccgccgct tgctccttga cgaaccgtcg cagaatggcg ttgaaatcga cgtagtcgtc
     2221 gttgcggaag cgctcgacac agagtgcgcg caatcccccg cccacaggct cggccagcgc
     2281 gagatagctt ttggtgccgc cgatgtccgc ggccagtacg atgtcgctca tgatctccga
     2341 ccgaacgccg cagcgaggac tgttatccta cgccagcctg cttactggga tgtgataccc
     2401 gcaaaacaat gaacggcgca acccctcgca tcctcttcgt cacgcccgag tgcgccccat
     2461 gggtgaaaac cggcggcctc ggcgacgttt ccgccgccct gcccgccgcg ttgcaggcgc
     2521 tcggctgcga cgtcgccatt ctcatgcccg cctatcgcgg catgcacgcc atcgcgcaac
     2581 gcgcgcagga agccgcgacg atccccgcac tcgcggaact gcccgctgcg cgcattctcg
     2641 aagcgcacct ggacaacggc gtcaccatgc tgctgctcga cgcgcccgcg ctctacgatc
     2701 gcgaagccgg cccttatctg gacgccaccg gccgcgactg gcccgacaat cacctgcgct
     2761 tcggcgcgct gtcgcatgcc gccgcagtgg ttgcgcgcaa cggtctcgcc ggcggatggc
     2821 ggcccgatct cgtgcattgc aacgactggc ctgcagcgct cgcgcccgcg tacctgcgtt
     2881 tcgaaccgcc gacggagccg gtcccgaccc tgctgaccat tcacaatctc gccttccagg
     2941 gtttgtttcc cgcgcaaacg cttgcacagc tcggtctgcc caagcacagc tacgacatcg
     3001 acggactgga gttctggggg caagtgtcgt tcctcaaggg cggcatctgc tacgccgacg
     3061 cgatcaccgc ggtcagcccc aactacgcgc gcgaaatcca gggcaaggaa ctgggcttcg
     3121 ggctcgacga actgctgcac gcgcgccgcg acgtgctgca cggcatcgtc aacggcatcg
     3181 ataccgatta ctggaacccg gcgaccgatc cgatgatcgc cgcgcaatac gacgtcggct
     3241 ggttcgacgc gaaaccgttc aacaaactcg cgctgcagaa acgcctgagc ctgccgcagg
     3301 attcgcgcat tccggtactc ggcacggtgg ggcggatcac gctgcagaag ggtttcgacc
     3361 tcatcgccgc catcgccgaa gaactgatgg agattcccgt ccatctggtc atgctcggct
     3421 ccggctcgct cgagatcgag gagcagttgc tgcggctgca gtccgcgtat ccgaaatgca
     3481 tcgggctgaa gatcggcttc gacgaaaagc tcgcccacat gatcgaagca ggcgccgaca
     3541 tgttcctcat gccgtcgcgc ttcgaacctt gcggcctgaa ccagatgtac agccagcgct
     3601 acggcacgcc acccgtcgcc aacgccacgg gcggactggt ggataccatc atcgactacg
     3661 acggcacgcc ggaaagcgcc gaacgcgcga ccggtttttt gttccacgaa gcgagccccg
     3721 aagcgctgct cgaagccatc cgccgtgcgc tggattgcta tcgcgagccc gaactgtggc
     3781 gcaaactgtg tttcaacggc atgaagacgg atttcagctg gcaggcgagc gcgaagcgct
     3841 acgtcgacct ataccgctcg cagattaaaa aaacttgaac cacagaggca cagagacaca
     3901 gagaacgtca aaaacgcagt ctgccgggga agcgattaca agctgcatcg tcatgccctg
     3961 tgaagcgagc atccagtgcc atgctgatcg ctttccggtc cgggttttct ctgtgtctct
     4021 gcgtctctgt ggtgaaggtt tgtgttttac tccgctctcc acgactctac tcacgcccag
     4081 atcatcaacc ccagttcgaa gggatgcgtg agcaggtcgt gggccggata ggcgcggatg
     4141 cggtaatcca agcgaccgca taactccggc gcaagatcga gcacgaagcg gtattcgccc
     4201 gcctcggtgc gcgtcccgtc ggcggcgaag gcatacacgc ggcgttcctc cgtctcgcgc
     4261 aatccccgcg tcaccaccgc ctcgatcgac acatcggccg gcgacagcgt gtccagatcg
     4321 accgccactt cgatgcgcat cgattcgccg aaacgaatgc gccgcttggg cgtatcgagc
     4381 cgccgcatgg cgacgttcgg ccaggccgcg cgcacctgcg ccttccactg cgcaatgagc
     4441 cgtgcgttgg cataaccgcc ttcggcgtac ttgcggccgc tttgcgaggc gggcagatag
     4501 aacttctcga catattcgtt gatcatgcgg cgcgcattga agcgcggcag gatggtggcg
     4561 atcgaacgct tggacatgcg cacccaatcg ggcgagtagc ccatgttgcc gcggttgtaa
     4621 tagagcggca cgacgttgtc ctgcaggatt tcgtacagcg tgtgcgcctc ttcccggtca
     4681 cgctgctgct cgctgctcgc ttcggtcgcc ggcttgattg cccagccgtt gtcgccgcaa
     4741 tagccttcgc cccaccagcc gtcgagcacc gacagattga tcacgccgtt catgccggcc
     4801 ttcatgcccg aagtgccgga ggcttccagc ggatagaccg gattattgag ccacacgtcc
     4861 acgcccgtgg tgagccggcg acccagacgc atgtcgtagc cttccagcag caggatgtgc
     4921 ccttcgaact cgggcatgcg cgatacgcga ctgaccgtgc ggatgaggtc ctgccccggc
     4981 acgtcggccg gatgcgcctt gccggcgaaa atgaacacca ccggccgctc cggatcgctg
     5041 acgatcttgt gcagcagttc gaggtcctgg aacagcagcg tggcgcgctt gtaggtcgcg
     5101 aagcgacggc cgaaacccac ggtcagcaca ttgggcgatt ccggatcgac gaaacgcagc
     5161 aggcgatcca gatgcgcctc gctcccctgg ttgcgatagt gctggatgcg gacgcgatga
     5221 cggaccagat gcagcatctg cgccttgaga tgctggcgcg tgctccagta gatgtggtcg
     5281 ggcatggcca tcagccgcgc ccacatttcc tcgtcgctgt ggcgcgtgcc ccagtcgaaa
     5341 gccatgaagc gctcgaaaat ggcgttccac tccggcgcga cgaaggtggg cgtgtgcacg
     5401 ccgttggtca cgtaggtgac cgggttctcg tttgccggca cctgcggcca caggtctttc
     5461 agcatttccg cactgacgcg accgtggatg cgcgatacgc cgttgtgatg gcgcgaaccg
     5521 cgcaccgcga gcgcggtcat gttgaagtcg gtgttgaagg gcgtgcggcc caacgcaagc
     5581 aagcgttccg gctccaggtc cagatcgccg cagtaggcct tgaagtactc gacgatcatc
     5641 tcttcggcga agtgatcgtg tccggctgcc acgggcgtgt gcgtggtgaa tacggtgttg
     5701 accgcgaccg cctccagcgc cgtggcgaag tccaggccct gcttcacgtg agcccgcgta
     5761 cgttcgagga cgagaaaggc cgcgtggccc tcgttgatat gccagaccgt cggcttgaga
     5821 cccaatgcgc caagcgcgcg cacgccgccg acgcccagca cgatctcctg ctcgatgcgc
     5881 gtggtgcgat cgccgccgta cagatgatga gcgatgtcgc gatcggattc gctgttcgat
     5941 tccaggtcgg tgtcgagcag atagagcgtc acatgaccga tgcgcgcctg ccacacctgc
     6001 acgtcgatca cgcgaccggg cagttccacc tgcacccgca gcggctcgcc gttgtcctgc
     6061 aacaccggcg tgacaggcag atcgtcgaaa tccgaatcct gataatgcgg ctgctggttg
     6121 ccctcgcggt cgatggtctg ggagaaataa ccctgccggt aaagcagccc cacgccgacg
     6181 aaaggcaatc ggatatcgct cgccgccttg caatggtcgc cggctagaat gccgagtccg
     6241 cccgagtaga tcggaaaact ttcgtgaaaa ccgaattcgg cgcagaagta ggcgaccaga
     6301 tccgtttgcc gcaaccactc cgaaccgttg cggcgcagcg gttcggagtg gtagctgtca
     6361 tatgcggaca gcacccggtt gaaggcgctc aaatacgccg gatcgtcggc cgcctcgaac
     6421 agacgctgct cgtccacgcg tttgagcatc gccttgggac tgtgtcccac cgcatcccat
     6481 agcggcggat gcagacgcgc gaacagcgag cgcgtggcgc gatcccagct ataccagagg
     6541 ttgttcgcca gttcctccag ccgcgccaga cggtgcggaa tattgggatt gacgtcgagc
     6601 gagtattcgg tgggggaaac catggtgcga gtcctagcaa gcgttgtgcg cggaagcgcc
     6661 gccgattcta gcggagagcc tccggcgacg gtgtagcatg ggcgagtcac gggaggccga
     6721 tatggaatcg tcgcgcatgt cgtccggcaa cgtcgtctcg cacaacctgc cgccgccgtt
     6781 ggcggatttc gacgcttggc aaagcgacgc cctgcttcgg gccgcggtcg cacgcgaagg
     6841 cgcggactgg atcgaagcgc gcgctgtccc gctcggccgg ctcgtcggca gcgtcgcatt
     6901 ccaggaatgg gcgacccagg ccaatacgca cccgccggtg ttgcaagcct acgatcgcaa
     6961 cggcgatcgc atcgacgtcg tcgactatca cccggcctat catcaactca tgcaggccgc
     7021 gttcggcgcg ggattgcatt cgctcgcctg gaaggaaacg cgcgaaggcc gctacgtcgc
     7081 ccgcgccgcc ctcaattact tgtggaatca gggcgagcag ggcaccgcct gtccggtgac
     7141 catgactttc gcgagcgtgc aggtgttgcg caacgcgccg gatctggcag cgcagtggga
     7201 agcgaagata ctcgccgacg attacgatcc gcgctccctg ccccttgcgc acaagcgcgc
     7261 cgcgaccatc ggcatggcga tgacggaaaa gcagggcggc tccgatctgc gcagcaacgc
     7321 cgccgtggcg attcccgtca gcggcgatga gcacgtactg ctcggccaca agtggttctg
     7381 ctctgcgccc atgagcgacg gttttcttac gctcgcgcag cgcagcgaag gacctacctg
     7441 tctgttcgtg ccgcgcatcc tgccggacgg gacgcgcaat tccttgcgaa tcgtgcggtt
     7501 gaaggacaag ctgggcaatc gctcgaatgc ttccgccgaa atcgaatacg agcgcacgca
     7561 cgcctggttg gtcggcgaac cgggacgcgg catcggcacg ctcatcgaga tggcgcatct
     7621 cacgcgcttc gatatcgtga tcgccgttgc cggcatgatg cgcgccgcgc tgcggcaagt
     7681 cctgcaccac acgcgccatc gcgccgcctt cggcgcgccg ctgatcgagc agccgctgat
     7741 gcgcaacgtg cttgccgatc ttgcgctgga gacggaagcc gcgatgctgc tcgccttccg
     7801 tcttgccggc gctttcgacc gcgccgcgca ggacgagcgc gcacgaatct acgcgcgcgt
     7861 cgtgacaccc gcagccaaat actggctgtg caagcgcctg ccgatgctgg cggccgaggc
     7921 gctggaatgt ttgggcggca acggctacgt ggaggattgg ccgatggcgc gcttctatcg
     7981 cgaagcgccg ctcaacggcg tgtgggaagg cagcggcaat gtcatttgcc tcgatgtgct
     8041 gcgcgcgctg gccaagacgc ccggcgcgct cgacatgctc gacgtcgagc tggcgcaggc
     8101 cgcaagacac cccgccatcg ccaggcacct cggcctgctg catcgtgccg tgcacgaaga
     8161 acccgcaggg ggcgaggcgc gccgcctcac cgaggggctg gccctttgcc tgcaggcggc
     8221 cctgctgctc gaacatgcac cgcccgcggt ggccgaagcc ttcatcgccg cgcgcctggg
     8281 cggccagggc ggactggctt tcggcacact gccggcggag accgattgcg ccgccatcat
     8341 tgcgcggcac cgcgaagctt gagcggcgca caccgatcga cggacgaagc gcaacgctgc
     8401 gctatgattc gcccatgaca ctcactgaac tccgttatgt ggtggcggtc gcgcgcgagc
     8461 gccacttcgg tcgcgccgca gaggcgtgtt tcgtcagtca gccgaccctg tcggtcgcgg
     8521 tgcgcaagct ggaagacgaa ctgggggtca ccctgttcga gcgcggttcg ggcgaagtcg
     8581 ccatcacgcc catcggcgaa cgggtgatcg aacaggcgca gcgcgtgctc gaacaggccg
     8641 ccatggtcaa ggaagtggcc aagcagggga aggatcaatt gaacggcgcc ctgcgcatgg
     8701 gggtcatttt caccatcggc ccgtatctcc tgcccgaact ggtaccgctg ctgaaaaagc
     8761 gtgcgccgcg catgccgctg atgctgcagg agcagtacac ccaccggctc atcgaactgc
     8821 tgcgccaggg cgagatcgac gtggccatac tcgccctgcc gttggaggcc aacggtctga
     8881 tggtgcaacc gctctacgac gaacccttcg tggtcgccgt gccgcgcgcg catccgtggg
     8941 ccgcgcgcaa aacggtttcc agccgcgaat tgaaggacga gcacatgctg ttgctcggca
     9001 ccggccactg cctgcgcgat caggtgctcg aagtctgccc cgaactcaat cgcttcagcg
     9061 ccacggccga cggcatccag aaatccttcg aaggctcgtc gctggaaacc atccgccaca
     9121 tggtggcttc gggcaccggc gtgaccgtcc ttcccgccac ggcggtgccg cgcggcgcca
     9181 aacgcgaccg gctgctcgcc tatatccctt tcagcaaacc cgttcccgac cggcgcgtgg
     9241 tgctcgccta tcgcaagacc ttcccacggc cggaagccat cgaagtcgtg cgcgaagccg
     9301 tgttgagctg cgatctgccc tgtgtggaaa tgctcgatct ggcaccggaa ccgtcctgac
     9361 ccggtgcggc gccgaagtcg agatagcgtt tggcaatcaa tatgttacaa agctttcatt
     9421 ggccaagtga aggtttaaac ggtatggtat aggcgagaga tcgacatagg agaagagtga
     9481 tggcgaagaa tacgcgcggt tatgcagcga acatcaatat cggcctgtcc gacaaggaac
     9541 gtatgaagat tgcggagggg ctgtcccgcc tgctggccga tacctacacg ctttatctga
     9601 agacgcacaa cttccactgg aacgtcaccg gtccgatgtt caacaccctg cacctcatgt
     9661 tcgaggagca gtacaccgaa ctggccaccg cggtcgacga aatcgccgag cgcatccgcg
     9721 cgctgggcgt gcccgcgccg ggcacctaca aggactacgc gcgactgtcc tccgtcaagg
     9781 agacggaagg cgtgccttcg gccaatgaaa tggttcagga cctggtggaa ggtcaggaag
     9841 ccgtgacgcg cacggcccgt gaagtgtttg aagtggcgga gaaggccaac gaccagccta
     9901 ccgccgacct gctgacgcag cgtatgcagg tgcatgaaaa aacggcctgg atgttgcgca
     9961 ccttgctgga gaagtaacgg ggcacggcaa gcgccgcccg taaagcatgt ttatgaaacg
    10021 gcctccattg ggaggccgtt ttgtttttct cgtcgccccc ttcggcatgc gctttagccg
    10081 ggttacttac actttctgga ttccggctcg cgcccttcgg gcttggccgg aatgacggca
    10141 tatttatcat cattgcggat agcagtagtc gcgtgatgat ccgcctttta ctcttccact
    10201 ctcgactctc aactgctttc tcaaagccgc gccaaatcca cttccgcctt tcccgcccag
    10261 gctttttgga agcgctgttg catcgccgcc gcctgtttcc gatcgccgcg tttttccagc
    10321 acttgcgtca gaccgtatag cgcccaaccg ttgttcggtg cgcgcaccag gctggtgcga
    10381 aatgcctgtt ccgcgccgtc cagatcgccc tgtgccagca acaccgcgcc cagcgattga
    10441 tgcagcgggt aataccaata aggcggttcc atataggcca ggtgctcctg cgccgcgatg
    10501 gccgcgcgaa agtgccgcgt cgcttgcggc aagtcgccgg ctgcctgcgc ggcgcgcgcg
    10561 cgtaccgtct gttcggcgat gcgcagcacc tgcggcaccg gaatcaacgc ctcctccaac
    10621 gccttgaaat cgctttcgcg attcagtctg gcgatggcat cggcttccgc acgcgccgcc
    10681 ggaatgtcac cggccgccgc atgggcggtc ccacgggcgt aatgccaaat cgccttgatg
    10741 taggggtagt cgtcgcccgg atcgggcagg gccagcacgg tggccggatc gctgaactgc
    10801 gcatgggcga aataagccgc caccttcacc ggatgaacca acggcacgtc gcgcaaggcc
    10861 tcgtcgggaa tgacggcgga caatttctcc gcggcggcga ccgccgtcgg tccgtcgccc
    10921 gccatctgcg ccgaagccat gaggaagtgc acgttgtgcg ggtaatagcc ccaggcgtaa
    10981 atgccttccg ctttcgactt cgacaaatag gcttcgtccg tcgcgacggc gcgcttgttg
    11041 gccgccagcg aatcgatata gcgccccacg cggtaataga tgtgcgaggg catatggacg
    11101 atatgtccag cacccggcat gagcttctcc agccgctcgg cgtgcgattc ggcacgcccg
    11161 ggccggttgg aagcctcgac ggcgtggata tagagatgga tcgcgcccgg gtgatcggga
    11221 tgggccttca gtacccgttc cagcacctgc acgatctcgg cgccgtggcc cttgggctgc
    11281 gcaccgccgg gctgccagta atcccagggc tgcgtatcca tcatcgcttc ggcataaagc
    11341 gtggcgatga tggtgtcctg cggaaagcgc cgatgcgcac ttgccatggc ctcggcatag
    11401 gcggcgtcca gcgccgcacg gtcggtcggg ggctgcgccg tatagcgcgt cgccagcgcc
    11461 tcgatcaagg cgcgttcgcg cacgctcgcc tgcggcgcaa gttgctgcgc cttcgtcaac
    11521 gcggccatgg ccggcgcgac ggcatcgggc gacatcggcg cattgatatt cgggccgagc
    11581 accaacgcct cgccccagta gcacatcgca caatccggat cccgtttctg tgcagcgcga
    11641 aaagcccgac gcgcttcgcc gtggttgaac gcgtgcgaca gacgcaggcc ctgatcgaaa
    11701 tactgctgcg cggccggcga gcgcgtagtg atcttgtaat gcagcgcgcc cagaccggcc
    11761 catagcggcg gatcgacgct ctcatcatcc ggtgtcggcg ccatttgcgg ccgatcgacg
    11821 gggcgcagtt ccgggctgcg cggcgactgc gcgagttgag cgagtcgagc cccgtagcgc
    11881 agcgcctggg ggcgagaaga gcccgacaga caaagaaccg ggtcgtcgag cagcggatcg
    11941 aaactcgcgg cgccgatcgc gccgcgatcg ccggcggcat aagccagcgt gccggaaacg
    12001 agtatgccgc cgaccgcgag cgcgatcggg gtgggggcgc ggcgtggact cacggcattc
    12061 ctccctggct ggcgggacag cgcccgaacg agcgcatcta gtgtctggta gacgcaaaaa
    12121 cgccgcaaag caaggtctta ttgatacgga ccgccgcctc cggcccgcca tgcgtacgca
    12181 ggccgatcaa gcgctcagac ccatcccgac cgccggcgcc cgacttgcaa cgtgggcggg
    12241 ttgtttgcat tggacgtcga gggcgcactc gccctggata acggaaagga attgttcgat
    12301 gcgtagcctg atcggcgtat ttctattagc actcctcctt gccggctgcg agcaacagcc
    12361 gtcggaacag accatggaag acgcgcggga atcgagcgcg gagatgcgcg aagacgcacg
    12421 cgatgcggcg gcagaggtgc gcgaggccgc cgaagacgcg gccgatgcga cacgggaagg
    12481 cgcacaggag ctgaaggagg aagcacgcga ggcgcgaaaa gacgcgaagt gatcgcgcga
    12541 tgcgcgtcgc atgcgccatt gtgcgcgtgc ggcgtgcgcg caacaggaca gtgaatattt
    12601 cactatgctg cgccgcaccg gtgaattggt tcttcttctt tgcgcgcttt tgtgtcaacg
    12661 tgtactcgtt gcagtgaagc gtgagttgca ttgagctttc atacgtcagt tgcagttgta
    12721 cacgtaacac catcggtcag catgccgagc cggctcgttt tatctctccc gcgcgccttg
    12781 cgcactcgtc ggttcgctta cgcatcattt cattcaatag gcatggaaag tgctgttttc
    12841 tcgcagcatg ccgcgcaacg aataagcgtt caatgcacca agagaagcgg cgattcatca
    12901 cgcgtggcta cacgcgatca cgcccgatgc gtcgcccgag cgctcgcgcc tcccctggat
    12961 tgcaagaacg ttgaggcaga gaaggaatcg aaggcttagg cggtccgtta ttacgacgcc
    13021 cgctcaaacg acggaggtag gcaaatggga gtcatcgaat catacgtctc gaattacgca
    13081 cggactcgcg aagcggaaat gacgctcgcc gagtatctgg atctgtgtaa gcgcgatccc
    13141 atggcctatg ccacagcggc cgagcgtatg ctcgccgcca tcggcgaacc ggaggtgatc
    13201 gacacacgta acgatccgcg cctgtcgcgc atcttctcca accgcgtgat ccggcgttac
    13261 ccggcgttca aggagttcta cggactggaa gacgtggtgg cgcagatcgt cgctttcttt
    13321 cgtcacgcgg cacaggggct ggaagagcgc aagcaggtgc tctatctgct cggcccggtg
    13381 ggcggcggca agtcctcgct ggccgaacgg ctcaaggcgt tgatggaggt caaccccatc
    13441 tatgcgctca agggttcacc ggtcaatgaa tcgccgctgg gcctgttcaa ccccgagcgc
    13501 gacgccgaca cgctggaggc cgagtacggc atcccgcgcc gctatctgca cggcatcatg
    13561 tcgccgtggg cggtgaagcg cctggaggaa taccagggcg acatctcgca gttcaaagtg
    13621 gtgcggctga tgccttcggt gctgcgccag gtggcgatca gcaagaccga accgggcgac
    13681 gagaacaatc aggacatctc ttcgctggtc ggcaaggtgg atatccgcaa gctcgagcat
    13741 tacgcgcagg acgatccgga cgcctacagc ttctccgggg gcctgtgcct ggcgaaccag
    13801 ggtctgctgg agttcgtcga gatgttcaag gcgccgatca agatgctgca tccgctgctg
    13861 acggcgacgc aggaaggcaa cttcaagggc acggaaggct tcggcgcgat tccgttccag
    13921 ggcatcatcc tggcgcactc gaacgagtcg gagtggcaga gcttccgcaa caacaagcac
    13981 aacgaagcct tcctggaccg ggtatacatc gtcaaggtgc cctactgcct gcaggtcacc
    14041 gaggaggtgc agatctatca gaagctgctg cgctcctcct ccctggccag cgcgccgtgc
    14101 gcgccgcata cgctggaaat gatggcgcag ttctcggtgc ttacgcgcct gaaggagccg
    14161 gagaactcca acatctactc caagatgcgc atctacgacg gcgagaacct caaggacatc
    14221 gacccgaacg cgaagacctt ccaggagtac cgcgactacg ccggcatcga cgaaggcatg
    14281 acgggcatgt ccacccgctt cgcctacaag gtgctgtcgt ccgtgttcaa ctacgaccag
    14341 acggagatcg cggccaaccc ggtgcatctg atgtacgtgc tcgaacagcg catcatgcgc
    14401 gaggacctgc cggaggaaac gaagcggcag tatctggcct tcatcaagga gcatctggca
    14461 ccgcgctatg ccgagttcat cggcaaggag atccagacgg cctatctgga gtcctactcg
    14521 gagtacggcc agaacatctt cgaccgctac gtcacattcg ccgactactg gattcaggac
    14581 gaggactacc gcgatccgga gacgggcgaa tccttcgacc gcgcttcgct gaacgcggaa
    14641 ctggagaaga tcgagaagcc ggccggcatc gccaacccga aggacttccg caacgagatc
    14701 gtcaacttcg tgctgcgcgc ccgcgcgcaa gggggcggca agaatccggc ttggaccagc
    14761 tacgagaagc tgcgcgaagt gatcgaacgc aagatgttct ccaataccga ggaactgctg
    14821 ccggtgattt ctttcaacgc caaagcctcg gcagaggatc ggagcaagca cgagaacttc
    14881 gtcgcgcgca tggtgcagaa gggctatacc gaaaagcagg tccggttgct gtgcgagtgg
    14941 tatctgcggg tacgcaagtc ggcctgaggc ggcttgggaa agctggaaac acggaggaca
    15001 cggagaacac cgagaacttc cagaacgatg ggaaccgcct gggagttgtt tcgctccgtg
    15061 ctctccgcgg ccctccgtgc tcttcgtgtt gagaggctgt gccatgtcga ccttcattga
    15121 tcgcagactg aacgaccggc gcaagagcgc cgtgaaccgg cagcgcttca tcgagcgcta
    15181 caaggaccac ttgcggcgcg cggtgagcga catcgtcgcc aaacgctcca tcaaggaact
    15241 ggaatcgggc ggacaggtgc agatccctgt caaggacatc accgaacccg ccttccgcca
    15301 cggcccgggc ggcgaccgcg aaatcgtgca cccgggcaac cgtacgtttg cgccgggcga
    15361 ccgcctgccc cgtcccgacg acggcgaagg cgacggccag ggcggcgccg ccggcgaagg
    15421 cgagatgctc gacagcttcg cgttcacgct gtcgcgcgag gaatttctcc agctctttct
    15481 cgacgatctg gagctgccgc gcctggcgcg tacggtactg ggcagcatca aggacaagcg
    15541 cagccagccg gcggggttca cccgcacggg ctcgccgcgc aacctccata tcgtgcgctc
    15601 gctcaagcag tcgctggcgc gccgcatggc tgcgcgcggc acggtgggcg ccctcgagag
    15661 gccagtcggc ggcggccggc gcgtgccttt cctcgacgaa gtggatttgc gctaccggca
    15721 tcgcgtcacc gtgcccaagc cggtgagtca ggccgtgatg ttctgtctca tggacgtctc
    15781 cggctcgatg accgaggaca agaaggatct ggccaagcgc ttcttcgcgc tgctctacct
    15841 gttcctgacg cgcaagtacg aaaaagtcga tctcgttttc gtgcggcaca ccgacgacgc
    15901 ctccgaagtc aacgaggaag agttcttcca cggcaattac agcggcggca cggtagtgct
    15961 ctcggcgctc gagttgatga aggagatcgt cgacgcgcgt tatccgccgg cgctgtggaa
    16021 catctacggc gcgcaggcgt ccgacggcga cgccttcggc gcggacccgg aaaaaagccg
    16081 cgggttcctg gagaaggaac tgctgccggc ctcccgctac ttcgcctaca tcgacatccc
    16141 gaccgaaggg ctggagcaga cgagcacgct gtggcaggcc tatcagcgca tcgatcagcc
    16201 gcacttcgcc atgaagcgcg tggcgaaccg gacggatatc tacccggtgt tccgcgaatt
    16261 gttccgcaag gaaacggtca agagctgagg tccgcgcgca ggagaacgac aatgaaaaaa
    16321 cccatctcga ccggctgcga ctggaacttc gaactgctgc aggaatacga cgaggcgatc
    16381 gccgacatcg ccatcaacga gtacaaactc gacgtctttc cgaaccagat cgaggtgatc
    16441 agctccgaac agatgctcga cgcctacgcc tccaccggca tgccgatcgg ctatcaccac
    16501 tggagcttcg gcaaggaata cctgcgccat gagcaggcgt accgcaaggg gatgcgcgga
    16561 ctggcctacg agctggtcat caactcgaac ccctgcatcg cctatctcat ggaggacaac
    16621 accatgacct tgcaggcgct ggtcatcgcc cacgcctgct acggtcacaa ctccttcttc
    16681 aagggcaact acctgttccg cagctggacg g
//
LOCUS       DATGOU010000111        10045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_6560319, whole genome shotgun sequence.
ACCESSION   DATGOU010000111 DATGOU010000000
VERSION     DATGOU010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10045
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6560319"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VKZ48_06520"
     CDS             <1..649
                     /locus_tag="VKZ48_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010929106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLU77535.1"
                     /translation="KVNAAGRLGGSADPVAARAQIEQSFAHVGKTPIDLIQVHNLGDV
                     PTQFGILKEYRDAGRVRYIGMTTTFEKQYDELERILRSERPDFIGVDYAIDNRVMEER
                     IFPLAQERGIGVLVYQPFGRARLWQLVKGHTVPAWAAEYDIASWGQFFLKFVISHPAV
                     TAATPATSQPRHVIDNMGAAVGRLPDEAGRRRMIEHIESLSARHKKTPLDGLSGV"
     gene            complement(689..2959)
                     /locus_tag="VKZ48_06525"
     CDS             complement(689..2959)
                     /locus_tag="VKZ48_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HLU77536.1"
                     /translation="MPKQLATRRSRARVGSGCALRARVFKVQPLLLALSLIPLGAAAQ
                     SETVLEEMVVSASGFEQEIKDAPASISVITREELETRQYRDLAEALTNVEGVDVRGAT
                     GKTGGLNISIRGMPSEYTLILIDGRRQNVAGDVTPNGFGDALTSFMPPVSAIERIEVI
                     RGPMSTLYGSDAMGGVVNIITRKVAKKWGGSVGFEVGIPEDSDWGDSRKMNFYASGPL
                     KEDLLGLQLRGNIYRRDGSELIAAPDVNVPTGRNPAPTETRQHTIGARLTLTPNKQHD
                     IWLDVEQGRTWYDNEECQLGNVDTKATGTGGSATCVGTPNPAVAPGYKEALRFNRDQY
                     AIGHTARLGIGLLESSLMRNVTETIGRTIPGNVIGNTYVTGGKTFTIGADRELKTTNT
                     VLDSKLVMPLGERHVATIGGQWWDAKLEDGLLPEKHDQTMWALFAEDEWRILDRLALT
                     LGARYDRHDVYGGEISPRAYLVWNTTDQWTIKGGVSKGFKAPRVDQLIDGVRGVGGQG
                     ANITIGNPNLEPEISTNTEIGALYDNRRGLTGSVTAFHNKIKDKITSGGVCSVSSISS
                     CAASPDPDAEYQINSDEAKTWGVEFATRIPLAKAWSLSLNYTWMDSELITDGEKNGRL
                     SDHPDHIANAQLRWDATQKLALWLRGEYRGESQRFDGDPKNLTGNNRLQYEALGDLKA
                     YTLFHLGGSYKVSKDVTLSANIFNLFDKDFRDFKRWTDDTGGVHWGSEYYQSGRSIKG
                     TTLSGRTFWIAANINF"
     gene            complement(3141..3821)
                     /locus_tag="VKZ48_06530"
     CDS             complement(3141..3821)
                     /locus_tag="VKZ48_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA];
                     GO_function: GO:0031418 - L-ascorbic acid binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HLU77537.1"
                     /translation="MLIQIPDVLSKEQVRQCREVLEAAEWVDGRATAGHQGAKVKKNR
                     QLPESSAAARQLGDLVLAALERNPLFISAVLPARVYPPLFNRYEGGETFGNHIDNAVR
                     LIPGTGQKIRTDVSSTLFLSEPEEYEGGELLIEDTYGTHTVKLPAGAMVVYPGTSLHR
                     VNPVTRGVRLAAFFWTQSMIRDDGQRTLLFDLDTSIQRLDRQQADEQALVQLTGVYHN
                     LLRMWAET"
     gene            complement(3870..6056)
                     /locus_tag="VKZ48_06535"
     CDS             complement(3870..6056)
                     /locus_tag="VKZ48_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006417007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HLU77538.1"
                     /translation="MKKNMGLRPTCAAVRAVFERTADHTPFAAAALVSLAMAPGLAAA
                     QTGETMTTLPRVEVQGQAIGGDYAAGETTVGAKTPTPVRDIPQVVNVVPRAVIDAQQA
                     NTLTDALRYVPGITLSAGEGGAIGDNINLRGQSARTDVFIDGFRDRGQYTRDTFFLES
                     VEVLKGPSSLFFGRGSTGGVVNQVSKAPSLRNHNDVGLTIGTEDYYRASVDINRAISE
                     TAAFRVAAFGHTSDSTRDVVESERYGIAPSLRFGIGTPTTVTLSSVHLRSEEIPDLGI
                     PTIQGTKANPGGPVHVDHDNFYGYTDDYFDQDVDAVTARIEHQFSPSLTLRNQTQYSS
                     ARIDTRATNIHLDTALNVWERGLRTREIDDTSLYNQTDLIAKFDTGGIKHTVVVGVEV
                     GRDEYERTSFGPSDGTVQDLQNPVYGPLPSHIVQLPSSVTETDATTLAVYANDQVELN
                     RHWKIVGGLRWDRFDVDQKAREVSTGDVTKLEQTDEVLSGRIGVVYQPTLTQSYYVSY
                     GNSFNPSAETISLSEANENVDPEENKSYEIGGKWDLLNGNLSLTAAIFRIEKSDARSL
                     DPVTGEVLLSGETQTDGFELGATGRILPNWQVFGGYTYLDGEIERLDERDGRTGPIVS
                     RDGNDLVNTPKHNFTLWTTYFLTPEWELGGGLVYSSKRTLNNANTAEVGSYTRYDLTL
                     AYHQKDYDIRLNVLNLTDKEYFEVASSSRAVPAQGRTALVTATYRF"
     gene            complement(6223..7623)
                     /locus_tag="VKZ48_06540"
     CDS             complement(6223..7623)
                     /locus_tag="VKZ48_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HLU77539.1"
                     /translation="MKPALIVLLASITLSGCALTSERPPMRLDMPADYRAEAPVATVA
                     LTQDWWRSFESSELAGLIDLALRDSPDLGMAVERVRQAEAQVRIAGSSLFPQLDLSAS
                     TNRRRTDPDRGSAVTTESTSATLSASYEIDAWGRNASGRRAAEALLDLSRFDEESLRL
                     TTVAGVANAYFQVLSLRGRLEVARENLAIAERVLDIVQIRARNGVVSQLDVARQNSAV
                     LSQRAAIPALALQERQTLAALAILVGQPPQHFDVKGADVGDVAVPVVAPGLPAELLVR
                     RPDLAAAEAQLVAANANLAAARAALLPSLQLTTSAGLANNALLSLANPSSTVYNFGAS
                     MLQSIFDGGRLRAQRDIAESRERELVESYRAAILSALADVEDALAATARTAEQQNFQA
                     AALEEARRALRLAEVRYREGADDLLSVLDAQRTLFSAEDQVALTRLARLQASVSLYTA
                     LGGGWEMPARMADAAP"
     gene            complement(7635..9620)
                     /locus_tag="VKZ48_06545"
     CDS             complement(7635..9620)
                     /locus_tag="VKZ48_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HLU77540.1"
                     /translation="MKSDLPIIEHALDPDTLPLIELVDVTKTYRNGDMAVQVLHGVSL
                     AIYPGEFVAIMGASGSGKSTLMNIIGCLDRPTTGQYRFMGEDVADFDRDELARLRREA
                     FGFVFQSYNLIGNATALENVEVPAVYAGVPRKERHVRAAELLATLGLDDRTEHRPTQL
                     SGGQQQRVSIARALMNGGQIILADEPTGALDSRSGEEVMALLKKLHAQGHTIIVITHA
                     KEVAEQAQRLIEIKDGYIVADPGPTLSPTANALRIQARDTGASRSMLNDAMEAAKTAA
                     RSLRANLFRTVLTLLGIVIGVASVIAMLAVGDGAKEQVLQRISAMGTDILLVRAQAGN
                     QRGYGGSIATLVPADAEAIDELPNVRAAVPEMTGNVTIRYGNRDYQTSAAATGAAFTD
                     ARNWFPVRGTFYSQEDVRTYAAVVALGQTVADALFPDGEDPIGRHVVINNVMFQVVAV
                     MGVRGASPWGTDLDDTILVPLTTGSLRLFGQRHLRSITVAVEDVSRIDETQAAVEELL
                     LARHGTEDFQVRNTASIIEMASETQNTLTMLLGSVAAISLLVGGIGVMNIMLVSVTER
                     TREIGIRMATGARMRNILQQFLTEAMVVSAVGGLLGVAIGLGAASIIQAFDTPVKFSI
                     EPVLLAFGCAFITGLAFGYMPARKAARLDPVQALSAE"
     gene            complement(9617..>10045)
                     /locus_tag="VKZ48_06550"
     CDS             complement(9617..>10045)
                     /locus_tag="VKZ48_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77541.1"
                     /translation="TNNVVLYDALFDVPNPDQKLMTQMTAQVFFVRASAKDVVIVPVS
                     ALQLMQQPAVGARRAAAQPGESRPAEARKQPSAATVLVIRPDGSVEERPIEVGVMTRV
                     YAQVRSGLEPGEVVAVGLKQADAPERAPQGPQQRRMRPSL"
BASE COUNT         1679 a         3243 c         3246 g         1877 t
ORIGIN      
        1 gaaagtgaac gccgccggcc gcctcggcgg atcggccgac ccggtcgctg cacgcgcgca
       61 gatcgagcaa tccttcgcgc acgtgggcaa gacccccatc gatctgatcc aggtccacaa
      121 cctgggcgac gtgcccacgc agttcggcat cctcaaggaa tatcgcgatg ccggccgggt
      181 gcgctacatc ggcatgacga ccaccttcga gaagcagtac gacgaactgg agcgcatcct
      241 gcgcagcgaa cgaccggact tcatcggggt cgactacgcc atcgacaatc gtgtgatgga
      301 ggagcggatc ttcccgctgg cgcaggagcg cggcataggc gtcctggtgt atcagccctt
      361 cggacgcgcc cgactatggc aactcgtcaa aggccatacg gtgccggcat gggcggccga
      421 gtacgacatc gcctcgtggg gccagttctt cctgaaattc gtcatctcgc atcccgccgt
      481 caccgccgcc acgcccgcta ccagccagcc ccgccatgtg atcgataaca tgggcgccgc
      541 agtcggacgt ctgcccgacg aggcggggcg ccggcgcatg atcgaacaca tcgagtcgct
      601 gagcgctcgc cataaaaaaa cgccgctcga tggactgagc ggcgtttgag gcatcgcaac
      661 ggccgttacc ggaacggccg ggcggtgatc agaagttgat gttggccgcg atccagaacg
      721 tgcgtccgga cagcgtcgtg cccttgatcg agcggccgct ttggtagtac tcgctgcccc
      781 aatgtacgcc gccggtgtcg tccgtccacc gcttgaagtc gcggaagtcc ttgtcgaaca
      841 ggttgaagat gttcgcgctg agcgtcacat ccttcgacac cttgtacgaa ccgccgaggt
      901 ggaacagcgt gtaggctttc agatcgccga gcgcttcgta ctgcaggcgg ttgttgccgg
      961 tcaggttctt gggatcgccg tcgaaacgct ggctttcgcc gcggtattcg ccgcgcagcc
     1021 acagcgccag cttctgcgtc gcatcccagc gcagctgtgc gttggcgatg tgatcgggat
     1081 gatcgctgag cctgccgttc ttctcaccgt cggtgatcag ttcgctgtcc atccaggtgt
     1141 agttgaggct caaactccat gccttggcga ggggaatgcg ggtagcgaat tccacgcccc
     1201 aggtcttggc ttcgtcgctg ttgatctgat attcggcatc cggatccggg ctggccgcgc
     1261 aactggaaat gctgctgacc gagcaaaccc cgccgctggt gatcttgtcc ttgatcttgt
     1321 tatgaaaggc cgtcaccgat ccggtcaaac cgcgacggtt gtcgtacagc gcgccgattt
     1381 cggtgttggt gctgatctcc ggctctaggt tcgggttgcc gatggtgatg ttggcaccct
     1441 gaccgccaac gccgcgcacg ccgtcgataa gctgatcgac tctcggtgcc ttgaagccct
     1501 tgctcacgcc gcccttgatg gtccattgat cggtcgtgtt ccatacgaga taggcccgcg
     1561 ggctgatttc accaccgtaa acgtcgtgcc ggtcgtaacg cgcacccagc gtcaacgcca
     1621 ggcggtcgag gatgcgccac tcgtcttcgg caaacagcgc ccacatggtc tgatcgtgct
     1681 tctccggcag caggccgtcc tcgagcttgg catcccacca ctggccaccg atggtggcga
     1741 cgtggcgttc acccagcggc atgaccagct tgctgtcgag tacggtattg gtggttttca
     1801 attctcggtc tgcaccaatg gtgaaagtct tgccgccagt cacgtaggtg ttgccgatca
     1861 cgtttccggg aatggtacgg ccgattgtct ccgtgacatt gcgcatcagg ctcgactcaa
     1921 gcaggccgat acccagccgg gccgtatggc cgatggcgta ctggtcgcga ttgaagcgca
     1981 acgcctcctt gtagccgggc gcgacagccg gattgggggt acccacacaa gtggcgcttc
     2041 cgccggtacc cgtggccttg gtatcgacgt tgcccagttg gcactcctcg ttgtcgtacc
     2101 aggtacgccc ctgctcgacg tcaagccaga tatcgtgctg cttgttgggc gtcaaagtca
     2161 gacgtgcgcc gatggtgtgc tgacgagtct cggtcggagc ggggttgcgt ccggtcggca
     2221 cgttgacgtc cggagccgcg atcagctccg agccgtcccg acgatagata ttgccgcgca
     2281 gttgcaggcc cagcagatct tccttcagcg ggccgctggc gtagaaattc atcttgcgcg
     2341 aatcgcccca gtcgctgtcc tcgggaatgc ctacctcgaa gccgacgctg ccgccccact
     2401 tcttcgccac cttgcgggtg atgatgttca ccacgccgcc catggcgtcc gaaccgtaga
     2461 gcgtggacat cggaccgcga atgacttcga tgcgctcgat ggccgacacc ggcggcatga
     2521 agctggtcag cgcatcgccg aagccgttgg gggtgacgtc accggcgacg ttctggcgac
     2581 ggccgtcgat cagaatcagc gtgtactcgc tcggcatgcc gcgaatgctg atgttcagcc
     2641 cgccggtctt gccggtcgcc ccgcgcacgt ccacgccttc gacgttggtc agcgcctcgg
     2701 ccaggtcgcg gtactgtcgc gtctccagtt cctcgcgcgt gatgacgctg atactggccg
     2761 gcgcatcctt gatctcctgt tcgaaaccgg acgcgctcac caccatttcc tcgagcacgg
     2821 tctcggattg cgcggcggcg ccgagcggta tcagggacag cgcgagcagt agtggttgaa
     2881 ctttgaatac ccgggcgcga agcgcgcagc ccgacccgac acgcgcgcgg ctgcgccgcg
     2941 tggccagttg ctttggcatt gatttctctc ccgactttct tgtttggtgt agcgactggc
     3001 tggctggccg ggaaaggctg actggcggcg ctggtacgaa tccgtctcgc agaaacgaga
     3061 aggattctca ttcagctacc gtcgaagcgt tgtgatttag gttaaatgcc ggacaaggcg
     3121 cggggcggcg agtcgagcgc tcaggtctcc gcccacatgc gcagcaggtt gtgataaacg
     3181 cccgtgagct ggaccagcgc ctgttcgtcc gcctgctgtc ggtcgagtcg ctggatcgac
     3241 gtgtcgagat cgaacagcag ggtccgttgc ccgtcgtcgc gaatcatgct ctgcgtccag
     3301 aagaaggcgg caagccgcac gccgcgggtg accgggttca cccgatgcag gctggtgcca
     3361 ggatacacca ccatcgcgcc ggcgggcagc ttgaccgtat gcgtgccgta ggtgtcttcg
     3421 atcagcagtt cgccgccttc gtattcctcg ggctcgctca ggaagagcgt cgatgaaaca
     3481 tcggtacgaa tcttctgccc cgtgccggga atcagccgca cggcgttgtc gatatggttg
     3541 ccgaaggtct cgccgccttc gtagcggttg aacagcggcg gatagacgcg cgcgggcagg
     3601 accgcgctga tgaacaacgg attgcgctcc agtgcggcca gcaccaggtc gcccagttgc
     3661 cgcgcagcag cggagctctc gggcaactgc cgattctttt tgaccttggc gccctgatgg
     3721 ccggccgtcg cccggccatc gacccactcg gccgcctcca gcacctcacg gcactgacgg
     3781 acctgttcct tgctcagcac gtcgggaatc tggatcaaca tcggtgaggc ctccgaatga
     3841 gacggcccgc attgcacggg ccgtcgaagc taaaagcgat aggtcgccgt cacgagcgcc
     3901 gtgcgcccct gcgccggcac cgcgcggctg gacgaggcca cttcgaagta ttccttgtcc
     3961 gtcagattga gcacattgag gcggatgtcg tagtccttct gatggtaggc cagcgtcaaa
     4021 tcgtagcggg tatagctgcc cacttcggcc gtattggcgt tgttcaaagt gcgcttcgag
     4081 gaatacacca ggccgccgcc cagctcccac tccggcgtga ggaagtaggt cgtccacagg
     4141 gtgaagttgt gcttgggcgt gttgaccaga tcgttaccgt cgcgcgaaac gatcggcccc
     4201 gtacgcccgt cgcgttcatc gaggcgctcg atttcgccgt cgaggtaggt gtaacccccg
     4261 aacacctgcc agttcggcag gatgcgcccg gtcgcgccga gttcaaaacc gtcggtctgg
     4321 gtctcgccac tcagcaggac ctctccggtc acggggtcga gcgaacgagc gtcgctcttc
     4381 tcgattctga aaatcgccgc cgtcagcgag aggttgccgt tcaacaaatc ccatttgccg
     4441 ccgatttcgt agctcttgtt ctcttcgggg tccacgtttt cattggcctc cgacagcgaa
     4501 atggtttccg cggacggatt gaacgaattc ccgtacgaga cgtagtagct ctgggtcaac
     4561 gtgggctgat agaccacacc gatacgtccg ctcagcacct cgtcggtctg ttcgagtttg
     4621 gtgacgtcgc cggtactgac ctcacgcgcc ttctggtcga cgtcgaagcg gtcccaacgc
     4681 aggccgccga caatcttcca atgcctgttc agttcgacct ggtcgttggc gtagacggca
     4741 agtgtcgtcg cgtccgtttc ggtgacggaa ctcgggagct gcacgatatg cgacggcaga
     4801 ggaccataaa ccgggttctg cagatcctgc accgtaccgt cactcggtcc gaaggaggtg
     4861 cgctcgtatt catcgcggcc gacttcaaca cccaccacga cggtgtgctt gatgccgccg
     4921 gtatcgaact tggcgatgag atcggtctgg ttgtagagcg acgtgtcgtc tatttccctc
     4981 gtgcgcaggc ctcgctccca cacgttgagc gcggtatcga ggtgtatgtt cgtcgcgcgc
     5041 gtatcgatgc gtgcactgct gtattgcgtc tggttgcgca gcgtcagact gggcgagaac
     5101 tggtgttcga tgcgcgcggt gaccgcgtcc acgtcctgat cgaagtaatc gtccgtatag
     5161 ccgtagaagt tgtcgtgatc gacatggacc gggccgccgg gattcgcttt ggtgccctga
     5221 atggtgggga tgccaaggtc cggaatctcc tcgctgcgca gatgcacgga cgacagggtg
     5281 acggtggtcg gcgtgccgat gccgaagcgc agcgaaggcg caatgccgta gcgctcggat
     5341 tccaccacgt cacgcgtcga atcgctcgtg tggccgaagg ccgcgacgcg gaatgcggcg
     5401 gtctccgaga tcgcccgatt gatgtccacc gacgctcggt agtagtcctc ggtgccaatg
     5461 gtcaggccca cgtcgttgtg gttgcgcagc gaaggcgcct tgctcacctg gttgaccacg
     5521 ccgccggtcg aaccgcggcc gaagaacagc gacgacggcc ccttcagcac ttccaccgat
     5581 tcgaggaaga aggtgtcgcg ggtgtattgg ccgcgatcgc ggaacccgtc gatgaatacg
     5641 tcggtgcgcg cactttgacc gcgcaggttg atgttatcgc cgatcgcgcc gccctccccg
     5701 gcgctcaacg ttatgcccgg cacatagcgc agcgcatcgg tcagggtgtt ggcctgctgc
     5761 gcgtcgatga ccgcgcgcgg caccacattc accacttgcg gaatgtcgcg caccggcgtg
     5821 ggcgtcttgg caccgaccgt cgtttcgccg gccgcgtaat cgccgccgat cgcctgcccc
     5881 tgcacctcca cccggggcag cgtggtcatc gtttcgcccg tttgcgcggc ggccaagccc
     5941 ggcgccatcg ccaggctgac cagcgcggcg gcggcaaacg gggtgtgatc ggcggtgcgt
     6001 tcgaaaacgg cgcgcactgc ggcgcaggta ggccgcaagc ccatgttttt tttcatctac
     6061 ctctcccaaa tctgtagcgg atggagcggc tggctggcta cttgggagag gctgactggc
     6121 gcgctgagat ttagcccgga tcacatccga ggcgtgcgcg aacgataatg gttctcgttt
     6181 agatcgtcaa agaaggggcg aaaatcctcg cattaacgca gcttatggcg ccgcgtcggc
     6241 catgcgcgcc ggcatttccc agccgccgcc cagcgcggtg tagagactca ccgaggcttg
     6301 caggcgggcc agacgggtca acgccacctg gtcttccgca ctgaacaatg tgcgctgcgc
     6361 atccagcacg gaaagcaggt cgtccgcgcc ctcgcgatag cgcacttcag ccagacgcag
     6421 ggcgcgacgc gcctcctcga gcgcggcggc ctggaagttc tgctgctcgg cagtgcgtgc
     6481 ggtggcggca agcgcatcct ccacatcggc cagtgcactc aggatcgcag cacgatagct
     6541 ttcgaccagt tcgcgttcgc ggctttcggc gatgtcccgc tgcgcgcgca ggcgtccgcc
     6601 atcgaaaatc gactgcagca tcgaggcgcc gaagttgtag accgtgctcg acggattcgc
     6661 cagtgacagc agtgcattgt tcgccagccc cgccgatgtg gtcagttgca ggctgggcag
     6721 caatgcggcg cgcgcggcgg cgaggttggc gttcgccgcg accagttgcg cttcggccgc
     6781 ggcgaggtct ggccgccgca ccagcagttc ggccggcagc cccggcgcca ccaccggcac
     6841 cgcgacatcg cccacatcgg cacctttgac atcgaaatgc tgcggcggct ggccgacgag
     6901 gatggcgagt gccgcgagcg tctgccgctc ctgcagcgcg agcgcgggaa tggcggcgcg
     6961 ctgcgacagc acggcgctgt tctgccgcgc aacgtccagt tgcgacacca cgccgttgcg
     7021 cgcgcggatc tgcacgatgt cgagcacgcg ctcggcgatg gccaggttct cgcgcgcgac
     7081 ctcgagccgg ccgcgcagcg acagcacctg gaagtaggcg ttggcgacgc ccgcgacggt
     7141 ggtcaaccgc aggctctcct cgtcgaagcg cgacaagtcg agcagcgctt cggcggcgcg
     7201 gcggccggag gcgttgcggc cccacgcatc gatttcgtag ctggcgctca aggtggcgct
     7261 cgtgctttcg gtggtaaccg ccgaaccgcg gtcgggatcg gtgcggcggc gattggtgga
     7321 ggcgctcaga tccagttgcg ggaacagcga cgagcctgcg atgcgcactt gcgcctcggc
     7381 ctgccgcacg cgctccaccg ccatgccgag gtcggggctg tcgcgcaggg ccagatcgat
     7441 cagcccggcc agttcggacg attcgaagct gcgccaccag tcctgcgtca atgcaacggt
     7501 tgcaaccggc gcttcggcgc gatagtccgc cggcatgtcg agccgcatcg gcggacgctc
     7561 gctggtgagc gcgcaaccgc tcaacgtgat gcttgccaag aggacgatca gtgcgggttt
     7621 catcaatcgc tccactactc ggccgacaac gcctgtaccg gatcgagccg cgccgccttg
     7681 cgcgccggca tgtagccgaa cgccagcccg gtaatgaagg cgcagccgaa agccaacagc
     7741 accggctcga tggaaaactt caccggcgta tcgaacgcct gaatgatgct cgccgcgccc
     7801 aaaccgatcg cgacgccgag cagcccgccg acggcggaga cgaccatcgc ctcggtgagg
     7861 aactgctgca ggatgttgcg catgcgcgcc ccggtcgcca tgcggatgcc gatttcgcgc
     7921 gtgcgctcgg taacactgac cagcatgatg ttcattacgc cgatgccgcc gaccagcagc
     7981 gaaatcgccg ccaccgagcc gagcagcatg gtgagcgtgt tctgcgtctc ggaggccatt
     8041 tcgatgatgg acgccgtgtt acgcacctga aaatcctccg tgccgtggcg tgcgagcagc
     8101 agttcttcca ccgccgcctg cgtttcgtcg atgcggctca cgtcctcgac cgcaaccgtg
     8161 atgctgcgca gatgccgctg gccgaacagc cgcagactgc cggtggtgag cggcacgaga
     8221 atggtgtcgt ccaggtccgt gccccagggt gatgcgccgc gcacgcccat gacggcgacg
     8281 acctggaaca tcacgttgtt gatgacgacg tgccggccga tcgggtcctc gccatcgggg
     8341 aacagcgcat cggccacggt ctgtccgagc gccacgacgg ccgcataagt ccgcacgtct
     8401 tcctgactgt agaaggtgcc gcgcacgggg aaccagttgc gcgcgtcggt aaatgcagcg
     8461 cccgtcgccg cggcggacgt ctggtagtcg cggttgccgt aacggatggt gacgttgccc
     8521 gtcatttcgg gcacagcggc gcgcacgttg ggcagttcgt cgatcgcctc cgcgtcagcc
     8581 ggcaccaatg tggcaatgct gccgccgtag ccgcgctgat tgcccgcctg cgcgcgcacc
     8641 agcaggatgt cggtgcccat ggcgctgatg cgttgcagca cctgctcctt cgcgccgtcg
     8701 ccgacggcga gcatggcaat caccgacgcg acaccgatca caatgccaag cagcgtcagc
     8761 accgtgcgaa acagattggc gcgcaaggaa cgcgcggccg tcttggccgc ctccattgcg
     8821 tcgttcagca tcgagcggct tgcgcccgtg tcgcgcgcct ggatgcgcag cgcattggcg
     8881 gtgggcgaaa gcgtgggccc cggatcggcg acgatgtaac cgtccttgat ttcgatgagc
     8941 cgctgcgcct gctcggccac ttccttggcg tgggtgatga cgatgatggt atggccctgc
     9001 gcatgcagct tcttcagcag cgccatgacc tcctcgccgc tgcggctgtc gagcgcgccc
     9061 gtaggctcgt cggccagaat gatctggccg ccgttcatca gtgcgcgcgc aatcgaaaca
     9121 cgctgctgct gaccgccgct cagttgcgtg ggccgatgtt cggtgcggtc gtcgagcccc
     9181 agcgtggcga gcagttcggc ggcgcgcaca tggcgctcct tgcgcggcac gccggcatag
     9241 acggccggca cctcgacgtt ctccagcgca gtggcgttgc cgatgaggtt atagctctgg
     9301 aacacgaagc cgaaggcctc gcggcgcaga cgcgcgagtt cgtcgcgatc gaagtccgcc
     9361 acgtcctcgc ccatgaagcg gtactggccc gtcgtcggcc ggtcgaggca gccgatgatg
     9421 ttcatcagcg tggacttgcc cgaaccggaa gcgcccatga tggcgacgaa ctcgcccgga
     9481 tagatggcga gactcacgcc gtgcagcacc tggaccgcca tgtcgccgtt gcgataggtc
     9541 ttggtgacgt cgaccagttc gatcaacggc aaggtatccg gatcgagggc gtgctcgatg
     9601 atgggcaggt ccgatttcat aggctggggc gcatgcgccg ctgctgcggt ccctgcggcg
     9661 cgcgctcggg cgcatccgcc tgcttgaggc cgacggcaac cacttcaccc ggctccaggc
     9721 cggagcgcac ctgcgcatat acgcgggtca tgacgccgac ttcgatggga cgttcctcca
     9781 ccgagccgtc cggacgaatg acgagcaccg tggccgcgga gggctgcttg cgcgcctcgg
     9841 cgggccgcga ttcgccgggt tgcgcggcgg cacggcgcgc acccacggcc ggctgctgca
     9901 tcaactggag cgcggaaacc ggcacgatca ccacatcctt cgccgacgcg cgcacgaaga
     9961 acacctgcgc cgtcatttgc gtcatcagct tctggtcggg gttgggcacg tcgaacagcg
    10021 cgtcgtagag cacgacgttg ttggt
//
LOCUS       DATGOU010000112        25712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_1233074, whole genome shotgun sequence.
ACCESSION   DATGOU010000112 DATGOU010000000
VERSION     DATGOU010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 25712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25712
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1233074"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..69
                     /locus_tag="VKZ48_06555"
     CDS             <1..69
                     /locus_tag="VKZ48_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HLU77542.1"
                     /translation="LRFAIREGGRTVGAGVVTKVIE"
     gene            231..545
                     /gene="rpsJ"
                     /locus_tag="VKZ48_06560"
     CDS             231..545
                     /gene="rpsJ"
                     /locus_tag="VKZ48_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HLU77543.1"
                     /translation="MALASQKIRIRLKAFDYKLIDQSALEIVDTAKRTGAVVKGPVPL
                     PTRIERYDVLRSPHVNKTSRDQFEIRTHLRLMDIIDPTDKTVDALMKLDLPAGVDVEI
                     KL"
     gene            702..1346
                     /gene="rplC"
                     /locus_tag="VKZ48_06565"
     CDS             702..1346
                     /gene="rplC"
                     /locus_tag="VKZ48_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019451019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HLU77544.1"
                     /translation="MSLGLVGRKVGMTRIFTDDGDSVPVTVLDVAGNRVAQVKKPETD
                     GYSAVQVVYGKRRANRVTKALAGHLAKAGVEAGTLTREFRVAESDAAELQPGAALNVD
                     LFQVGQKVDISGTTQGKGFAGVIKRHNFSSGRTSHGNSKAHRKPGSTGQAQDPGRVFP
                     GKKMPGHLGAVRATVQNLEVVRVDTERGLLLVKGAVPGSKGGHLIVRPAVKGAA"
     gene            1346..1966
                     /gene="rplD"
                     /locus_tag="VKZ48_06570"
     CDS             1346..1966
                     /gene="rplD"
                     /locus_tag="VKZ48_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011350675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HLU77545.1"
                     /translation="MDLKLINDQGQSASTVSASDTLFGRDYNEALIHQIVVAYQANAR
                     LGTRAQKGRGEVNKSKKKPWRQKGTGRARAGKASSPLWRGGGKIFPASPNENFSQKVN
                     KKMYRAGMASILSQLVREDRLKVVEDFKVEAPKTKLLAQKVKNLGLDEVLLLTDNIDE
                     NLYLSSRNLPNVLVLDVTEADPVALVHFKNVLLTKQAVARMEELLA"
     gene            1963..2286
                     /gene="rplW"
                     /locus_tag="VKZ48_06575"
     CDS             1963..2286
                     /gene="rplW"
                     /locus_tag="VKZ48_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HLU77546.1"
                     /translation="MSTQFAQDRLMQVLLAPVISEKSTYVGEKNNEVVFRVARDATKP
                     EIKAAVELLFSTKDKKVEVKGVRVSNVKGKEKRFGRFTGRRDHWKKAYVSLAEGSDVN
                     FHATE"
     gene            2290..3117
                     /gene="rplB"
                     /locus_tag="VKZ48_06580"
     CDS             2290..3117
                     /gene="rplB"
                     /locus_tag="VKZ48_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HLU77547.1"
                     /translation="MALVKVKPTSPGRRSLIKVTTPGLHKGKPHAALLEKKSKTAGRN
                     NTGRITTRHIGGGHKQHYRKVDFRRNKDGVPAKVERIEYDPNRSAHLALLCYVDGERR
                     YIIAPKGVTAGMELVSGSEAPIKVGNALPLRNIPVGSTIHCIEMQPGKGAQLARSAGA
                     GVQLLAREGSYAQLRLRSGEVRKVHVDCRATIGEVGNEEHQLKSVGKAGRTRWKGIRP
                     TVRGVAMNPVDHPHGGGEGKTAAGRDPVSPWGQHTKGLRTRKTKRTSGMIVSRRYKK"
     gene            3127..3402
                     /gene="rpsS"
                     /locus_tag="VKZ48_06585"
     CDS             3127..3402
                     /gene="rpsS"
                     /locus_tag="VKZ48_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HLU77548.1"
                     /translation="MARSIKKGPFVDAHLQKKVDAARANNDKRPIKTWSRRSTVLPDF
                     IGLTIAVHNGKQHMPVYISENMVGHKLGEFALTRTFKGHSADKKAKR"
     gene            3412..3762
                     /gene="rplV"
                     /locus_tag="VKZ48_06590"
     CDS             3412..3762
                     /gene="rplV"
                     /locus_tag="VKZ48_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HLU77549.1"
                     /translation="METQAKLYGVRLSAQKGRLVADQIRGKSVEQALNILTFSPKKSA
                     AVIKKVLESAIANAEHNDGADIDELKVTRIMVEQGMTYKRFRPRAKGRGVRILKPTCH
                     IFVTVGDDDRVEKA"
     gene            3772..4674
                     /gene="rpsC"
                     /locus_tag="VKZ48_06595"
     CDS             3772..4674
                     /gene="rpsC"
                     /locus_tag="VKZ48_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HLU77550.1"
                     /translation="MGQKINPTGFRLAVNKNWNSRWFANSKNFSTMLNEDLKVREYLK
                     KKLAHASVGRVVIERPAKDARITIHSARPGVVIGKKGEDIEILKTELRKLLGVNAVHV
                     NIEEIRKPEIDAQLIADSITQQLEKRIMFRRAMKRAMQNAMRLGAQGIKIMSSGRLNG
                     IEIARTEWYREGRVPLHTLRADIDYGTSEAKTTYGVIGVKVWVYKGNMLGKGEVPAAA
                     PVEEEQRRGRRGPGRPGGGRDGAPGAGPRGGAGRGRAARKDEPEVKTEEAAPAAAPAA
                     DAAKKPAAKRVVKKATGANDASTS"
     gene            4658..5074
                     /gene="rplP"
                     /locus_tag="VKZ48_06600"
     CDS             4658..5074
                     /gene="rplP"
                     /locus_tag="VKZ48_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013340739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HLU77551.1"
                     /translation="MLQPARTKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRITA
                     RQIEAARRAMTRHIKRGGRIWIRIFPDKPISTKPAEVRMGNGKGSPEYWVAEIQPGKV
                     LYEMDGVDEELARAAFALAQAKLPIRTTFVQRQIGA"
     gene            5078..5272
                     /gene="rpmC"
                     /locus_tag="VKZ48_06605"
     CDS             5078..5272
                     /gene="rpmC"
                     /locus_tag="VKZ48_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HLU77552.1"
                     /translation="MKASELRAKDEAQLNEELLSLLKAQFSLRMQQGTQQLTNHSQLK
                     RTRRDIARVRTILAEKARAK"
     gene            5305..5553
                     /gene="rpsQ"
                     /locus_tag="VKZ48_06610"
     CDS             5305..5553
                     /gene="rpsQ"
                     /locus_tag="VKZ48_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HLU77553.1"
                     /translation="MPNTLTGRVVSDKMDKTVTVLVERRVKHPLYGKYITRSKKYHAH
                     DETNEIKEGDLVTIQECRPISKSKAWKVTQLVEKSRAV"
     gene            5715..6083
                     /gene="rplN"
                     /locus_tag="VKZ48_06615"
     CDS             5715..6083
                     /gene="rplN"
                     /locus_tag="VKZ48_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HLU77554.1"
                     /translation="MIQMQSVLDVADNTGARAVMCIKVLGGSKRRYAGIGDIIKVSVK
                     DAAPRGRVKKGEIYNAVVVRTAKGVRRPDGSLVKFDSNAAVLLSNKLEPIGTRIFGPV
                     TRELRTEKFMKIVSLAPEVL"
     gene            6094..6411
                     /gene="rplX"
                     /locus_tag="VKZ48_06620"
     CDS             6094..6411
                     /gene="rplX"
                     /locus_tag="VKZ48_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HLU77555.1"
                     /translation="MQKIRKGDDVVVIAGRDKGKRGTVLRRIDKEHVVVEGVNRVKKH
                     VRPNPMKGLQGGIIDKDMPIHVSNVALFNPQTQKADRVGFKSLEDGRTVRVFKSNGEM
                     VDA"
     gene            6424..6963
                     /gene="rplE"
                     /locus_tag="VKZ48_06625"
     CDS             6424..6963
                     /gene="rplE"
                     /locus_tag="VKZ48_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HLU77556.1"
                     /translation="MARLQEFYRDTVMADLTKQFGYKSVMEVPRIQKITVNMGVGEAV
                     QDKKILDNAVADLTKLTGQKPVVTKARKSVATFKIRQGYPIGCMVTLRGRRMYEFLDR
                     LVTIAMPRIRDFRGVSGRAFDGRGNYNLGVKEQIIFPEIEYDKIDALRGMNISITTTA
                     KTDEEARALLAAFKFPFRN"
     gene            6976..7281
                     /gene="rpsN"
                     /locus_tag="VKZ48_06630"
     CDS             6976..7281
                     /gene="rpsN"
                     /locus_tag="VKZ48_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HLU77557.1"
                     /translation="MAKTAVINRELKRRKTVAKFAAKRAELIATIKDQNASDEDRAAA
                     RAKLQALPRNASPVRLRNRCELTGRPRGVFRKFGLGRNKLREIAMRGEVPGLTKASW"
     gene            7293..7688
                     /gene="rpsH"
                     /locus_tag="VKZ48_06635"
     CDS             7293..7688
                     /gene="rpsH"
                     /locus_tag="VKZ48_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HLU77558.1"
                     /translation="MSMTDPISDMLTRIRNAQASGKTTVSIPASKLKAAIAQVLKDEG
                     YVEDFAVHANEGKPVLEVNLKYYAGSPVIEKIERVSRPGLRIYKGAQEIPKVMNGLGV
                     AIVSTSKGVMTDRKARATGIGGEVLCIVA"
     gene            7701..8234
                     /gene="rplF"
                     /locus_tag="VKZ48_06640"
     CDS             7701..8234
                     /gene="rplF"
                     /locus_tag="VKZ48_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HLU77559.1"
                     /translation="MSRVGKSPIELPKGVEVTLAADAVSVKGPLGSLRQPLNGGVRVK
                     QEGEQLLVETTNDSKEANALSGTTRSLIANMVQGVSQGFERKLTLVGVGFRAQAQGDK
                     LNLALGFSHPVVHQMPQGVKVETPSQTEIVIKGIDKQVVGQVAADVRAYRPPEPYKGK
                     GVRYADEHVVIKETKKK"
     gene            8250..8603
                     /gene="rplR"
                     /locus_tag="VKZ48_06645"
     CDS             8250..8603
                     /gene="rplR"
                     /locus_tag="VKZ48_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013434799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HLU77560.1"
                     /translation="MDKKQARVRRAAQTRRKIHELRVARLSVSRTNQHIYAQLISADG
                     QKVLTSASTLEKEVRTQVPNGGNVNAASAVGKRIAEKAKQLGIEEVAFDRSGYKYHGR
                     VKALAEAAREGGLKF"
     gene            8620..9141
                     /gene="rpsE"
                     /locus_tag="VKZ48_06650"
     CDS             8620..9141
                     /gene="rpsE"
                     /locus_tag="VKZ48_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008642951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HLU77561.1"
                     /translation="MARMQPKMQPNDDRSDGLKEKMISVNRVTKVVKGGRILGFAALS
                     VVGDGDGGVGMGKGKAREVPVAVQKSLEEARRKLFRVSLRNGTLQHAVVGRHGASVVL
                     MQPASEGTGIIAGGPMRAVFEVVGITNVLAKCIGSTNPYNVVRATLNGLKSMNTPAEV
                     AAKRGKTVEEILG"
     gene            9152..9334
                     /gene="rpmD"
                     /locus_tag="VKZ48_06655"
     CDS             9152..9334
                     /gene="rpmD"
                     /locus_tag="VKZ48_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HLU77562.1"
                     /translation="MADKKIKVTLVKSVIGCKQKHRETVRGLGLRKINQTVELEDTPA
                     VRGMINRVNYLVRAEG"
     gene            9339..9776
                     /gene="rplO"
                     /locus_tag="VKZ48_06660"
     CDS             9339..9776
                     /gene="rplO"
                     /locus_tag="VKZ48_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HLU77563.1"
                     /translation="MELNTLKPAPGAKKARRRVGRGIGSGFGKTAGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFTSLTRNDVAEVRLSELEDLQAEKVDLATLKAAGIVP
                     RLALGAKVILSGELKRKVDLVGLKATKGARAAIEAAGGSIADA"
     gene            9801..11123
                     /gene="secY"
                     /locus_tag="VKZ48_06665"
     CDS             9801..11123
                     /gene="secY"
                     /locus_tag="VKZ48_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HLU77564.1"
                     /translation="MAKNVPQLGKTGKYGDLKRRLLFLLGALVVYRIGAHVPVPGIDP
                     QALSDLFQQNQGGILGMFNMFSGGALSRFTIFAIGIMPYISAAIIMQLMTAVSPTLEA
                     LKKEGAAGQRKITQYTRYGTLGLALFQSTAIAIALESMPGLVLEPGMMFRFVTVVTLV
                     TGTMFLMWLGEQITERGLGNGISIIIFAGIVAGLPSAIGGTLELVNTGAMHALTALLI
                     IAAVVVVTWFVCFVERGQRKILVNYAKRQVGNKIYGGQSSHLPFKLNMAGVIPPIFAS
                     SIILFPATIVGWFGQGEATWLRDIAATLSPGQPIYILLYAAAIVFFCFFYTALQYNPR
                     ETADNLKKSGAFVPGIRPGEQTSKYLERILLRLTLVGAIYVTLVCLLPEFLILKWNVP
                     FYFGGTSLLIIVVVTMDFMAQVQAYIMTHQYESLLKKANFKGSGFPTR"
     gene            11123..11341
                     /gene="infA"
                     /locus_tag="VKZ48_06670"
     CDS             11123..11341
                     /gene="infA"
                     /locus_tag="VKZ48_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HLU77565.1"
                     /translation="MSKEDVIQMQGEVIENLPNATFRVKLENGHVVLGHISGKMRMHY
                     IRILPGDKVTVELTPYDLSKARIVFRAK"
     gene            11370..11483
                     /gene="rpmJ"
                     /locus_tag="VKZ48_06675"
     CDS             11370..11483
                     /gene="rpmJ"
                     /locus_tag="VKZ48_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HLU77566.1"
                     /translation="MKVLASVKKICRNCKIIRRNRTVRVICKDPRHKQRQG"
     gene            11545..11922
                     /gene="rpsM"
                     /locus_tag="VKZ48_06680"
     CDS             11545..11922
                     /gene="rpsM"
                     /locus_tag="VKZ48_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020203842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HLU77567.1"
                     /translation="MARIAGVNIPNHQHAEIAITAIYGIGRARAKAICAAANVAGSTK
                     IKDLSDADMERLREQVAKFTVEGDLRREVSMNIKRLMDLGCYRGLRHRRGLPVRGQRT
                     RTNARTRKGPRKAAIAGKAQPGK"
     gene            11965..12363
                     /gene="rpsK"
                     /locus_tag="VKZ48_06685"
     CDS             11965..12363
                     /gene="rpsK"
                     /locus_tag="VKZ48_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HLU77568.1"
                     /translation="MAKPGQTASRVRKKAKKNVAEGIAHVHASFNNTIITITDRQGNA
                     LSWATSGGAGFKGSRKSTPFAAQVAAEQAGRMAQECGVKNLEVRIKGPGPGRESAVRA
                     LNALGLKITSISDVTPVPHNGCRPPKKRRI"
     gene            12378..13004
                     /gene="rpsD"
                     /locus_tag="VKZ48_06690"
     CDS             12378..13004
                     /gene="rpsD"
                     /locus_tag="VKZ48_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HLU77569.1"
                     /translation="MARNLDPKCRQCRREGEKLFLKGEKCFTDKCAIERRSYPPGQHG
                     QKQSRPSDYANQLRAKQKIRRIYGVLESQFHRTYTEAARRKGVTGETLLQLLESRLDS
                     VAYRMGFGSSRTEARQVVRHNGVLVNGKRVNIPSYQVRPGDVIEVAEKAKAQLRVKGA
                     AEAAESRGFPEWIEVDIKALKGTFKAVPQRSELPTTINENLVIELYSK"
     gene            13062..14048
                     /gene="rpoA"
                     /locus_tag="VKZ48_06695"
     CDS             13062..14048
                     /gene="rpoA"
                     /locus_tag="VKZ48_06695"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018440459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HLU77570.1"
                     /translation="MQSTSFLKPRIIDVQAIAPYHAKVVMEPFERGYGHTLGNALRRV
                     LLSSMPGFAPTEVQIAGVLHEYSTLEGVQEDVVDILLNLKGVVLKLHSGSEAMLRLNK
                     EGEGPVTAADIEVTHDVEVINPEHVIAHLAPGGKLEMSIKVERGRGYQPGNVRLVPEE
                     SKGIGRIILDASFSPVRRVSYQVESARVEQRTDLDKLIMDIETNGVIEPEEAIRYAAR
                     VLVDQLSVFAALEGTPVTVDTERKAPQVDPILLRPVDDLELTVRSANCLKAENIYYIG
                     DLIQRTETELLKTPNLGRKSLNEIKEVLASRGLTLGMKLENWPPAGLETARG"
     gene            14091..14486
                     /gene="rplQ"
                     /locus_tag="VKZ48_06700"
     CDS             14091..14486
                     /gene="rplQ"
                     /locus_tag="VKZ48_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HLU77571.1"
                     /translation="MRHRHGLRKLNRTSAHRLAMLRNMTNSLLKHEVIKTTLPKAKEL
                     RKVVEPMITLGKKPTLANRRLAFDRLRDRDMVIKLFDELGPRYAARNGGYLRILKYGF
                     RDGDNAPMALVELVDRPEPTEAESEQAAA"
     gene            complement(14733..14912)
                     /locus_tag="VKZ48_06705"
     CDS             complement(14733..14912)
                     /locus_tag="VKZ48_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77572.1"
                     /translation="MTVLVELIVAMLQVASILVLLYGLSLTIDNGLNKALTFAASKVR
                     QGVSRTKIDGVANAA"
     gene            complement(15175..16446)
                     /locus_tag="VKZ48_06710"
     CDS             complement(15175..16446)
                     /locus_tag="VKZ48_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008645998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HLU77573.1"
                     /translation="MNPRQLLIDSFNAAVAAADPLKILPAHLPEPPKGRTLVIGAGKA
                     AASMALAVEQHWPASAPLEGLVITRYRHGLLTNRIKVIEAGHPVPDESGEKAAREILR
                     LVKGLGPDDLLLALVSGGGSSLLSLPVEGITMDELKATTKELLRCGAPIQDMNTVRKH
                     LSVVQGGRLAAATKARVLALVVSDVTGDDPTHIASGPCAPDPTTYQDALDIFARYSVN
                     APAAVVDYLERGARGEIPETPKPGDPIFDRVENRVIATAQQSLQAGAEFFRQHGITPV
                     ILGDSVTGEASEVAKVYGALARQVDTYGDPFKRPVALISGGECTVTVRGNGRGGRCSE
                     FLLSLAIDLDSLPEVYALAADTDGIDGSEDNAGAVLSPDTLQRAEQKGLRAKQLAATN
                     DAYSFFDALGDLIITGPTRTNVNDFRVILLR"
     gene            complement(16631..17116)
                     /locus_tag="VKZ48_06715"
     CDS             complement(16631..17116)
                     /locus_tag="VKZ48_06715"
                     /inference="COORDINATES: protein motif:HMM:NF016156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HLU77574.1"
                     /translation="MRPRTAHCAILRAPLRLAVALEHPHNRPGFAFEATMPGTTKFFL
                     LFGAFAGMTGVMLGAFGAHALRGKLAPDLLSVYQTAVQYQFWHALALLAIGALAFHLP
                     ASAPLRWSGYAMALGILLFSGSLYALAFSGVRPLGMITPFGGITWIIGWALLAWAILR
                     A"
     gene            17144..18184
                     /locus_tag="VKZ48_06720"
     CDS             17144..18184
                     /locus_tag="VKZ48_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011615053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLU77575.1"
                     /translation="MEYNLLPGTDLRVSRICLGTMTWGEQTDAGDAHAQLDLAVARGI
                     NFIDTAEMYPIPPCAETQGRTERIFGDWLARQQRDRLIVASKVAGPGRREWLRGGRTE
                     LTKANIAEAIDGTLKRLRTDYIDLYQIHWPDRAVPMFGKTMFVPREEPQATPIIEQVE
                     AMEALVRAGKIRYWGLSNETTWGVCEYVRVARLHGLPQPVTVQNVYNLVSRKFDEELA
                     EACHREKLALLAYSPLAGGALTGKYRNNALPEGARYTLFPDFGTRFRKPMVAPAIDAY
                     HDIAASRGMTLTQLAIGFALSRWHTGAAIIGASGLAQLEENLAYADAPPLDEATLAEI
                     DAVHERYPSPAH"
     gene            18188..18499
                     /locus_tag="VKZ48_06725"
     CDS             18188..18499
                     /locus_tag="VKZ48_06725"
                     /inference="COORDINATES: protein motif:HMM:NF023171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HLU77576.1"
                     /translation="MTEAATLLVLLALVAWYWYDSMRARELALQLGRAACARDSLQLL
                     DDTVQCVRLRFARGEDGRLKLKRTYRFEFSDTGTNRRDGSIVLHGTAVESLYMEPFLV
                     Q"
     gene            18523..19266
                     /locus_tag="VKZ48_06730"
     CDS             18523..19266
                     /locus_tag="VKZ48_06730"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HLU77577.1"
                     /translation="MGKWIEGTVIGQRQWTDRLYSLQVKADLPSFEAGQFIKLALAVN
                     GEMVARPYSFVNAPKERPYEFYYIVVPEGPLSPALTKLEPLDNIYFAAQPSGFLVLSE
                     VPDCDHLWMLSTGTGIGPFLSILKTEAPWKRFKKIVLVHAVRHGAELTYRDQIEPLLE
                     AYPGQLQLISFVSREERPGALTGRIPEAITDGRLEKAAGLKLAPEDTHVMMCGNPEMI
                     SAVTALLKARGMKKHRRRDRGHITVENYW"
     gene            19311..19982
                     /locus_tag="VKZ48_06735"
     CDS             19311..19982
                     /locus_tag="VKZ48_06735"
                     /inference="COORDINATES: protein motif:HMM:NF017672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HLU77578.1"
                     /translation="MNWQQALDLYCERTDASFWSEPLNALSNAAFLVAAVLLARRARA
                     DGGRAPRDIDALAVLIALIGFGSFTFHTVATVWASLLDVLFIQIYIYVYLARYLRRIV
                     GVNLVWMYGGLVLYWLFEQAVTGGFEPGALNGSYRYLPALLALAGMTLYARRVHVPAA
                     APLAVAVGLLCVSIVLRTIDLGVCHLNPLGTHFFWHLINAAVLYYAAVALLRSEAAGR
                     YPRPI"
     gene            20027..21211
                     /locus_tag="VKZ48_06740"
     CDS             20027..21211
                     /locus_tag="VKZ48_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLU77579.1"
                     /translation="MEAAKKNVAVLAALQALLFTNNSTAIALNGLVGYALADNKALAT
                     LPVTGWVVGAALTTYLASMLMRRIGRRAGFTVGTCIGIVGASLACLAIYLGNFWLFCS
                     GTVVFGVYNAFGQYYRFAAADAAPGEFKSRAISLVLAGGLVGGIIGPTTSQWTKDLLP
                     VAYLGAYLTLVVFLLVVLVVLRLLDIPPPAAAVRQDSGRPLRQLAAQPAFMVAVLAGA
                     VGYGVMNLLMTATPLAMDLCGHSYGAAANVISWHVIGMFGPSFFTGSLIARYGVLRIL
                     LIGVALMLVCIAVALAGIAVANFWLALVLLGVGWNFLYIGGTTLLTETYRPAEQAKAQ
                     GANDMLIFVTMAVSSFSSGMLLEVNGWQMLNYLAIPFVVAVGVAVLWLMALRRRTAPL
                     AT"
     gene            21208..22173
                     /locus_tag="VKZ48_06745"
     CDS             21208..22173
                     /locus_tag="VKZ48_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLU77580.1"
                     /translation="MKQKLDPQVAALLERMARTGTPPYRQLGVDAARELYRMTRFTVG
                     VPHKEPAQTEDLAAEGPAGAIPLRLYRPAKAGADEVLPCVVYFHGGGWVLGDLSTHDE
                     VCRGLTNLAHCAVVAVDYRLAPEHKFPAAVEDAIAATQWIVEAAGRLRIDASRVAVAG
                     DSAGGTLATVTAIAARDAGGPPLVMQALIYPSTDMTEGTESHELYAEGYGLTRENILW
                     FREHYLSCEADACDWRASPLLTRDLSGLPPAYILTAGFDPLRDEAWQYAQRLQEAGVR
                     VTYECFEGMIHGFIIMGGAIAAARHAIYRVAQALRPALSTAPVRL"
     gene            22189..22581
                     /locus_tag="VKZ48_06750"
     CDS             22189..22581
                     /locus_tag="VKZ48_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLU77581.1"
                     /translation="MAIRAMNHFTVLSADLDATRVFYCDLLGLRVGPRPPLGFPGLWL
                     YAGDQAVLHVIGGRELPAQRSGVIDHMAFTAENLAQIVDALQARDIKYSLRRMPQEGG
                     WQLFCRDPEGALVELDFAAEEPAPTGHA"
     gene            complement(22592..23020)
                     /locus_tag="VKZ48_06755"
     CDS             complement(22592..23020)
                     /locus_tag="VKZ48_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77582.1"
                     /translation="MNRLSSAWKRLSPPLCALLLCAAAPPADAVDRGPIERNLLIHQQ
                     QSDSVALRNRQAAAELRARGSSAEEQSALRSLHAQQRREQDSLHSRQQRRLDALRMRP
                     DAPGTHATQRLEREHMERESRSQLQHFERARPYAVRPIRP"
     gene            23152..23943
                     /locus_tag="VKZ48_06760"
     CDS             23152..23943
                     /locus_tag="VKZ48_06760"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF003380.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HLU77583.1"
                     /translation="MSLRRVKRALGLRASVEVSEKRGVRTLHLGGDAIQSAIRLSAPD
                     ELELSYTRAMMSWLLFQPKPREVLMIGLGGGSLARFVHERMPDVRITAVEINEQVVAA
                     AHSFFGLPFEDKRLKVKIADGGKYVPAHRNACDVLLLDAFDDGRSVPALATQAYYDAC
                     RACLRPGGVLVVNFIRDEPRFDAYLSRIERSFDGKVLLLPCEDRVNNIVLAFKDGPVR
                     LAIDPLKRAARALKKRYRLPFDAYVRDLLYYNGKTAAFLRIGLER"
     gene            complement(23940..24911)
                     /locus_tag="VKZ48_06765"
     CDS             complement(23940..24911)
                     /locus_tag="VKZ48_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4892 domain-containing protein"
                     /protein_id="HLU77584.1"
                     /translation="MARLAARLCCMIFLLCAATGLWAADLPGAKDHPMVSRYQGSEII
                     KYEQRAFDEVHLIGGPIKQRGGRDRNPDAYRTVEGKFTRIAYHAPSGRSALEVFRNYE
                     QALQAGGFELLYRCDNEVCGGRPFNEAATPADLHTFMAYNEKDQRYLLARLARPTGDV
                     YAAVYVVRAYSIGGPNKDRVYANLQIVESQAMQQGMVKVDANAMAKGLDAEGHIALYE
                     IYFDTDKAELKPASAGALSEIAKLLSTQPKLKVLIVGHTDNVGALDYNRSLSERRAQA
                     VVDALVKQHKIARDRLTAVGVGMAAPIASNDGEAGRAKNRRVELVKR"
     gene            25024..>25712
                     /locus_tag="VKZ48_06770"
     CDS             25024..>25712
                     /locus_tag="VKZ48_06770"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007829520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HLU77585.1"
                     /translation="MDALQQARIDLAVALRWAARLDLSEGVCNHFSLAAPGMPGHFLI
                     NPQGFHWSEITPRHILVVDVKGKVVEGDYTVEPGAFWIHSRIHNSERAHKCIMHTHMP
                     YATALCITEGGRLEPASQNALRYYGRIAYDEEYNGLVTSEELGDRFLQKMGDAEIVFL
                     ANHGVIVCGDTVAAAFEDLYYLERACMLQVIAQSTGKPLKRISHKVASETVKLMVEDR
                     EQAEAFIAACKR"
BASE COUNT         5009 a         7962 c         8316 g         4425 t
ORIGIN      
        1 ttgcgctttg ccatccgcga aggcggccgc accgtcggcg ccggcgtggt cacgaaagtc
       61 atcgagtaaa gcgaacattg caggaaaatt tcggggtcgg cgcagtatcc gaccccgttg
      121 aatgaaggtt ccggggcatc ctcggagccg cttttttagc aagacaatcg ggcgctgggt
      181 gcgtataatc ccggcccttc gttctttgac agattcgagc aggaaagagc atggcccttg
      241 ccagtcagaa aatccgcatt cgcctcaagg cgtttgatta caagctgatc gaccagtccg
      301 cactggagat cgtcgatacg gccaagcgca ccggcgcggt agtaaagggc ccggttcccc
      361 tgccgacgcg catcgagcgc tatgacgtgc tgcgcagccc gcacgtcaac aagacctcgc
      421 gcgaccagtt cgagatccgc acgcatctgc gcctgatgga catcatcgac ccgaccgaca
      481 agacggtcga cgcgctgatg aaactcgacc tgcctgcggg cgtcgacgta gagatcaagc
      541 tttagaacct gagttcatcg agcttcgcga caaaccgcgc ggctcgttaa gtcagtagtg
      601 atttagcagt acaacgagcg tagcgaacac tagcgaagct cggctttact tttaaaacca
      661 gccaaccaat tgtagttggc actccgttag gagaaataac aatgagtctg ggactcgtcg
      721 gccgcaaggt cggcatgacc cgcattttca ccgacgacgg cgattccgtc ccggtgacgg
      781 tgctggacgt tgccggcaat cgtgtggcac aggtgaaaaa gcctgagacg gacggctata
      841 gcgccgttca agtcgtatat gggaagcgtc gcgccaatcg cgtgaccaag gcgctggcgg
      901 gccatctggc caaggctggt gtcgaggccg gtacgctgac ccgcgaattc cgcgttgccg
      961 agagcgatgc cgccgagctg cagcccggcg ccgcactgaa cgtcgacctg ttccaggtcg
     1021 gccagaaagt ggatatctcc ggcacgacgc agggcaaggg ctttgccggc gtcatcaagc
     1081 ggcacaactt ctcgtccggc cgtacctcgc acggcaactc caaggcccac cgcaagcccg
     1141 gttcgaccgg tcaggcgcag gacccgggcc gcgtgttccc cggcaagaag atgcccggcc
     1201 acctcggtgc cgtgcgtgcc actgtgcaga atctcgaagt cgtgcgcgtc gatacggagc
     1261 gcggcctgct gctggtgaag ggcgctgtac ccggctccaa gggcggccat ctgatcgtgc
     1321 ggcccgcagt gaaaggagcc gcgtaatgga tctcaaactg atcaacgatc aaggtcagag
     1381 cgcctcgacc gtgtcggctt ccgacacgct gttcggccgc gactacaacg aggcgctgat
     1441 tcaccagatc gtcgtcgcct accaggcgaa cgcgcgcctc ggcacgcgcg cccagaaggg
     1501 tcgcggcgag gtcaacaagt cgaagaagaa gccgtggcgt cagaagggca ccggccgcgc
     1561 gcgcgccggc aaggcgtcca gcccgctgtg gcgtggaggc ggcaagatct ttccggccag
     1621 ccccaacgag aatttcagcc agaaggtcaa caagaagatg tatcgcgccg gcatggcgtc
     1681 gatcctctcg cagctggtgc gcgaagaccg tctgaaagtg gtcgaggact tcaaggtcga
     1741 agcccccaag accaagctgc tcgcgcagaa ggtcaagaat ctgggtctcg acgaagttct
     1801 gctgttgacc gacaacatcg acgagaacct gtatctgtcg tcgcgcaacc tgcctaacgt
     1861 gctggtgctc gacgtcaccg aagccgatcc ggtcgcgctg gtgcacttca agaatgtgct
     1921 gctgaccaag caggcggtcg cccggatgga ggaactgctg gcatgagtac ccaattcgca
     1981 caggaccgcc tgatgcaggt gctgctcgcc cccgtgattt cggagaagag cacatacgtc
     2041 ggcgagaaga acaatgaagt ggtgttccgc gtcgcgcgcg acgcgaccaa gccggaaatc
     2101 aaggcggcgg tcgaactgct gttctcgacc aaggacaaga aggtcgaggt gaagggcgtg
     2161 cgcgtctcga acgtcaaagg caaagaaaag cgtttcggcc gcttcaccgg ccggcgcgac
     2221 cactggaaga aggcgtacgt gagcctggct gaaggcagcg acgtgaactt ccacgcgacg
     2281 gagtaagcca tggccctggt aaaagttaaa ccgacttcgc ccggtcgtcg ttccctgatc
     2341 aaggtgacga cccccggtct gcataagggc aagccgcacg ccgcgctgct ggagaagaaa
     2401 tccaagacgg cgggccgcaa caacaccggc cgcatcacca cgcgccacat cggcggcggt
     2461 cacaagcagc actatcgcaa ggtcgatttc cgtcgcaaca aggacggcgt gccggccaag
     2521 gtcgagcgta tcgagtacga cccgaaccgc agcgcgcacc tggcgttgtt gtgctacgtg
     2581 gacggcgagc gtcgctacat catcgcgccc aagggcgtga cggcgggcat ggaactcgtc
     2641 agcggttcgg aagcgccgat caaggtcggc aacgccctgc cgctgcgcaa catcccggtc
     2701 ggctcgacca tccactgcat cgaaatgcag cctggcaagg gtgcgcaact ggcgcgctcc
     2761 gcgggcgcgg gcgtacaact gctcgcccgc gaaggcagct atgcgcagtt gcgtctgcgt
     2821 tccggcgagg tgcgcaaggt gcacgtcgat tgccgcgcca ccatcggtga agtcggcaac
     2881 gaagagcatc agctcaagag cgtcggcaag gccggccgca cgcgctggaa gggcatccgc
     2941 ccgacggtgc gcggcgtggc gatgaacccg gtcgatcacc cgcacggtgg cggcgagggc
     3001 aagacggcgg cgggtcgcga cccggtgagc ccgtgggggc agcacaccaa gggtctgcgc
     3061 acgcgcaaaa ccaaacgcac cagcggcatg atcgtcagcc gccgttacaa gaagtaagga
     3121 taggacatgg ctcgttcaat caaaaaaggc ccgtttgtcg acgcgcatct gcagaagaag
     3181 gtggatgcgg cgcgtgcgaa caacgacaag cgtccgatca agacctggtc ccgtcgctcg
     3241 accgtgctgc ccgacttcat cgggctcacc atcgcggtgc acaacggcaa gcagcacatg
     3301 ccggtgtaca tctccgagaa catggtcggt cacaagctgg gcgagttcgc gctgacccgc
     3361 actttcaagg gtcactccgc ggacaagaag gccaagcgct aagaggacga catggaaact
     3421 caagcgaaac tttatggcgt gcgtctgtcg gcgcagaaag gtcggctggt agccgatcag
     3481 atccgcggca agtcggtgga gcaggcgctc aacatcctga cgttcagccc gaagaagagc
     3541 gctgcggtca tcaagaaggt gctcgagtcg gcaatcgcca atgccgagca caatgacggc
     3601 gcggatatcg acgaattgaa agtcacccgc atcatggtcg agcagggcat gacgtataag
     3661 cgcttccgcc cccgcgccaa gggccgcggc gtgcgcatcc tcaagccgac ctgccacatt
     3721 ttcgtgaccg tcggcgacga cgaccgcgtc gagaaggcct aaggagcaac gatgggacag
     3781 aaaattaatc cgaccggctt ccgtctcgcg gtcaacaaga actggaattc gcgctggttt
     3841 gccaacagca agaatttctc gacgatgttg aacgaggacc tgaaggtccg ggaatatctg
     3901 aagaagaaac ttgcgcacgc ttccgtgggc cgcgtggtca tcgagcgtcc ggccaaggat
     3961 gcgcgcatca cgatccacag cgcgcgtccg ggcgtggtga tcggcaagaa gggcgaggac
     4021 atcgaaatcc tcaagaccga gctgcgcaag ctgctcggcg tcaatgccgt gcacgtcaac
     4081 atcgaggaaa tccgcaagcc ggaaatcgat gcgcagttga tcgccgactc gatcacccag
     4141 cagctggaga agcggatcat gttccgccgc gccatgaagc gcgcgatgca gaacgcgatg
     4201 cgcctcggcg cccagggcat caagatcatg agctcgggcc gtctgaacgg catcgagatc
     4261 gcgcggaccg agtggtaccg cgaaggtcgc gtgccgctgc acaccctgcg tgcggacatc
     4321 gattacggca cctcggaggc caagaccacc tacggcgtca ttggcgtcaa ggtgtgggtc
     4381 tacaagggca acatgctggg caagggcgaa gtgcccgcgg cagcgccggt ggaggaagaa
     4441 cagcgtcgcg gtcgtcgcgg cccgggccgt cccggtggcg gtcgtgacgg cgcgcccggt
     4501 gcgggtccgc gtggcggtgc cggccgtggc cgtgcggcgc gcaaggacga acccgaagtg
     4561 aaaaccgaag aagccgctcc ggcggcggcg cccgcagcgg acgcggccaa gaagcccgcc
     4621 gccaagcgcg tagtgaagaa ggcgacagga gcgaacgatg cttcaaccag ctagaaccaa
     4681 ataccgcaag cagcagaaag gccgtaacac cggcatcgcc acacgcggca acaaagtgtc
     4741 cttcggcgaa ttcggcttga aggccgtcgg ccgcggtcgc atcactgctc gccagatcga
     4801 ggcggcacgt cgcgcgatga cccgccacat caagcgtggc ggccggatct ggatccgcat
     4861 cttccccgac aagccgatct ccaccaagcc cgccgaagtg cggatgggta acggcaaggg
     4921 cagtccggag tactgggtcg ccgaaatcca gcccggcaag gtgctgtatg agatggacgg
     4981 tgtcgacgag gaacttgcgc gtgcggcctt cgctttggcg caggccaagc tgccgatccg
     5041 cacgaccttc gtgcagcgtc aaattggagc gtaagcgatg aaagcatctg aattgcgcgc
     5101 caaggacgag gcgcaactga acgaagagct gttgagcctg ctgaaagcgc agttttcgct
     5161 gcgcatgcag cagggcaccc agcagctgac gaaccacagc cagctcaagc gcacccgccg
     5221 cgacatcgcg cgcgtacgca cgattctggc ggaaaaggcg agggctaaat aatggccgaa
     5281 cagaatcagg cggtggccga gaagatgccg aatacgctga ccggccgtgt ggtcagcgac
     5341 aagatggaca agaccgtcac agtgctggtc gagcggcgcg tgaagcaccc gctgtacggc
     5401 aagtacatca cccgctcgaa gaagtatcac gcgcacgacg agacgaacga gatcaaggaa
     5461 ggagacctgg tgaccatcca ggaatgccgg ccgatctcca agtcgaaggc gtggaaggtg
     5521 acccaactgg tggagaagtc gcgcgcggtg taaaaaccgc ccaccagggc ttgcgcggcg
     5581 ttgccgcgct cgctacaatc tcgcgttttt cgccgtggca cgtctcctct ggccacggcg
     5641 tccagcccga acgggctcca agactgaccg tcagtccggt ctgccgggcg gcgggttaag
     5701 ttggagaaag agaaatgatc caaatgcagt ccgtattgga cgtcgccgac aacaccggcg
     5761 cgcgtgcagt gatgtgtatc aaggtgctgg gcggctccaa gcggcgctac gccggcatcg
     5821 gcgacatcat caaggtcagc gtcaaggacg cggccccgcg cggccgcgtc aagaagggcg
     5881 aaatctacaa cgcagtggtc gtgcgcaccg ccaagggcgt gcgccggccg gatggctcgc
     5941 tggtcaagtt cgattcgaac gcggccgtgc tgctgtcgaa caagctcgag ccgatcggca
     6001 cgcgcatctt cggacccgtc acccgcgaac tgcggacgga gaagttcatg aagatcgtgt
     6061 cgctcgcgcc ggaagtgctg taaggagcgc gcgatgcaaa agattcgcaa gggcgacgat
     6121 gtggtggtga tcgctggccg tgacaagggc aagcgtggca ccgtgctgcg tcgcatcgac
     6181 aaggaacacg tcgtggttga gggcgtcaac cgcgtcaaga agcacgtccg gccgaacccg
     6241 atgaaggggc tgcagggcgg catcatcgac aaggacatgc cgatccacgt ctcgaacgtg
     6301 gcgctgttca acccgcagac ccagaaagcc gatcgcgtgg gtttcaagtc gctcgaggac
     6361 ggccgcaccg tgcgggtgtt caagtcgaac ggcgagatgg tcgacgcata agggatcgag
     6421 aagatggctc gcttgcaaga gttttacaga gataccgtaa tggccgacct gaccaagcag
     6481 ttcggctaca agtcggtgat ggaagtcccg cgcatccaga agatcacggt caatatgggc
     6541 gtgggcgaag cggtgcagga caagaaaatc ctcgataacg ccgttgccga cctgaccaaa
     6601 ctcaccggcc agaaacccgt ggtcacgaag gcgcgcaagt cggttgcgac cttcaagatc
     6661 cggcagggtt atcccatcgg ctgcatggtg accctgcgcg gtcgccgcat gtacgaattc
     6721 ctcgaccgcc tggtgacgat cgcgatgccg cgtatccgcg acttccgtgg cgtctccggt
     6781 cgcgccttcg acggccgcgg caactacaac ctgggcgtca aggaacagat cattttcccc
     6841 gagatcgagt acgacaagat cgacgcgttg cgggggatga acatcagcat caccacgact
     6901 gcgaagaccg acgaagaagc gcgcgcgctt ctcgccgcct tcaaatttcc gttcagaaac
     6961 taaagggttg cggatatggc caagactgcc gtcatcaacc gcgagctgaa gcgccgcaag
     7021 actgtggcga agttcgccgc caagcgggcc gaactgatcg ccaccatcaa ggaccagaac
     7081 gcctcggacg aagaccgcgc cgctgcacgc gcgaaactcc aggcgctgcc gcgcaatgct
     7141 tcgccggtac gcctgcgcaa ccgttgtgaa ctcaccggtc ggccgcgcgg cgtattccgc
     7201 aagttcggcc tgggccgtaa caagctgcgc gaaatcgcca tgcggggcga agtgcccggt
     7261 ttgaccaagg ccagctggta aggagtcgac gaatgagtat gacagacccg atttccgaca
     7321 tgctgacccg catccgcaac gcgcaagcga gcggaaagac caccgtgtcg atacccgctt
     7381 ccaaactgaa ggccgccatt gcgcaggtgc tgaaggatga aggctacgtc gaagacttcg
     7441 cggtgcacgc caatgagggc aagccggtgt tggaagtcaa tctcaagtat tacgccggat
     7501 cgcccgtgat cgaaaagatc gagcgagtca gtcgtcccgg cctgcgcatc tacaagggcg
     7561 cgcaggaaat cccgaaggtc atgaacggcc tcggcgtggc gatcgtctcg acctccaagg
     7621 gcgtaatgac cgatcgcaag gcccgcgcca ccggtatcgg cggcgaagtg ctctgcatcg
     7681 tggcctaaga ggtaacggac atgtcacgtg taggtaaatc tcccatcgaa ctgcccaagg
     7741 gcgtcgaagt gacgcttgct gccgacgccg tttcggtcaa gggcccgctg ggttcgctgc
     7801 gtcagccgct gaacggcggc gtgcgggtca agcaggaagg cgagcagttg ctggtcgaaa
     7861 cgaccaacga cagcaaggaa gcaaacgcgc tgtcgggcac gacgcgctcg ttgatcgcca
     7921 acatggtgca aggcgtcagc cagggtttcg aacgcaagct gaccctggtc ggcgtgggct
     7981 tccgcgcgca ggcgcagggc gacaagctga atctggcgct cggtttctcg catccggtcg
     8041 tgcatcagat gccgcagggc gtgaaagtgg aaacgccgag ccagaccgag atcgtgatca
     8101 aaggcatcga caaacaagtg gtcggtcagg tggctgcgga tgtgcgcgcg tatcgtccgc
     8161 cggagcccta taagggcaag ggcgttcgtt atgccgacga acacgtggtc atcaaagaga
     8221 ccaagaagaa gtaaggggtc cggaaaatga tcgacaagaa acaagcccgt gtgcgtcgcg
     8281 ccgcgcagac gcgccgcaag attcacgagt tgcgcgtcgc acgactgtcg gtgagccgca
     8341 ccaatcagca catctacgcg cagctgattt ccgccgatgg gcagaaggtg ctgaccagcg
     8401 cctcgaccct cgagaaggaa gtgcgtacgc aagtgcccaa cggcggcaac gtcaatgcgg
     8461 ccagcgcggt cggcaagcgc atcgccgaaa aagcgaagca gctgggcatc gaggaagtcg
     8521 ccttcgatcg cagcggctac aagtaccacg gccgcgtgaa agcgctcgcc gaggccgccc
     8581 gcgaaggcgg cctgaagttc taaggatacg aggaccgata tggcaagaat gcagcccaag
     8641 atgcagccga acgacgaccg ttcggacggc ctcaaggaaa agatgatttc ggtgaaccgc
     8701 gtcaccaagg tggtgaaggg cggccgtatt ctcggcttcg ccgcgttgtc tgtcgtgggt
     8761 gacggcgacg gtggcgtcgg catgggcaag ggcaaggcac gtgaagtgcc ggtcgccgtg
     8821 cagaagtcgc tcgaggaagc gcgccgtaag ctgttccgtg tcagcctgcg caacggcacg
     8881 ctgcagcatg cggtggtcgg tcgtcacggc gcgtcggtgg tgctcatgca gcccgcctcc
     8941 gaaggtaccg gcatcatcgc cggcggcccg atgcgcgcgg tgttcgaggt ggtcggcatc
     9001 accaacgtgc tagccaagtg catcggctcg accaacccct acaacgtcgt gcgcgccacg
     9061 ctcaacggcc tgaagtcgat gaacacgccg gcggaagttg cagccaagcg cggcaaaacc
     9121 gtcgaagaga tcctgggtta aggagcgcag catggcggac aagaaaatca aggtgacgct
     9181 ggtcaaaagc gtcatcgggt gcaagcaaaa gcaccgcgag accgtgcgcg gcctgggcct
     9241 gcgcaagatt aaccagacgg ttgaactgga agatacgccg gcggtacgcg gcatgatcaa
     9301 ccgcgtcaac tacctcgtcc gcgcggaagg ctaaagcgat ggaactgaat actctcaagc
     9361 ccgctccggg agcgaagaaa gcgcgccgcc gcgtcggccg cggcatcggc tccgggtttg
     9421 gcaagaccgc gggtcgcggt cacaagggcc agaagtcgcg ttcgggcggc ttccacaagg
     9481 tcggtttcga aggcggtcag atgccgctgc agcgtcgtct gccgaagcgg ggtttcacct
     9541 cgctgacgcg taacgacgtc gccgaggtgc gcctgtccga actggaagac ctgcaggcgg
     9601 agaaggtcga tctggcgacg ctgaaggcgg ccggcatcgt cccgcgtctg gcgctgggcg
     9661 ccaaggtgat tctgtccggc gaactcaagc gcaaggtaga cctggtcggc ctcaaggcga
     9721 ccaagggcgc ccgggcagcg atcgaagcgg ccggcggcag catcgccgac gcctgatagg
     9781 cagcgaacgc ggtaaacact ttggccaaga acgttccaca actgggcaag accggaaagt
     9841 acggcgacct caaacggcgc ctgttgttcc tgctgggcgc gttggttgtt taccgcatcg
     9901 gggcgcatgt gccggtgccg gggatcgacc cgcaggcgct gtcggacctg tttcagcaga
     9961 accagggcgg tatcctgggg atgttcaaca tgttctccgg cggagcgctg tcgcgtttta
    10021 cgattttcgc gatcggcatc atgccgtaca tttccgcggc catcatcatg cagttgatga
    10081 ccgcggtgtc gccgacgctg gaggcgctca agaaagaggg cgcggccggg cagcggaaga
    10141 ttacgcagta cacgcgctac ggcacgctgg gtctcgcgct gttccagtcc acggcgatcg
    10201 ccattgcgct ggagtccatg ccgggtctcg tgctcgagcc ggggatgatg ttccgcttcg
    10261 tcaccgtggt gacgctggtg acgggcacga tgttcctgat gtggctgggt gagcagatca
    10321 ccgagcgagg gctgggcaac ggcatttcga tcatcatctt cgccggtatc gtggcgggat
    10381 tgccgagcgc gatcggcggc acgttggaac tggtgaacac cggggcgatg catgcgctga
    10441 cggcgctgct gatcatcgcg gcggtggtgg tcgtgacctg gttcgtgtgt ttcgtcgagc
    10501 ggggacagcg caagatcctg gtgaactacg ccaagcgcca ggtgggcaac aagatttacg
    10561 gcggccagag ctcgcatctg ccgttcaagc tgaacatggc cggggtgatt ccgccgatct
    10621 tcgcgtcgtc gatcattctg ttcccggcga ccatcgtcgg ctggttcggg cagggagagg
    10681 cgacctggtt gcgggatatc gcggcgacgc tatcgccggg gcagccgatc tacattctgc
    10741 tgtatgccgc ggcgattgta tttttctgct ttttctacac ggcgttgcag tacaacccgc
    10801 gtgaaacggc ggacaacttg aagaaaagcg gtgcgttcgt gccgggtatc cgcccgggcg
    10861 agcagacgtc gaagtatctg gagcggattc tcctgcggtt gaccctggtc ggtgcgattt
    10921 acgtcaccct ggtctgcctg ctgccggagt ttctgatcct gaagtggaac gtgccgttct
    10981 acttcggcgg cacctcgctg ctgattattg ttgtcgtgac gatggatttc atggcgcagg
    11041 tccaagccta catcatgacc caccagtacg agagcttgtt gaagaaggcc aatttcaagg
    11101 gatcaggatt tccgacgcgc tgatgagcaa agaagatgtc atccagatgc agggagaggt
    11161 cattgagaac ctcccgaacg caaccttccg agtgaaactc gagaacggac acgttgtgct
    11221 gggtcacatc tccggaaaga tgcgcatgca ctacatccgc atcctgcccg gcgacaaagt
    11281 gaccgtggaa ctcacgccat acgatttgag taaggcccgg atcgtcttcc gcgcgaaata
    11341 gcggcggacg acctcagtag gagagaaaaa tgaaagtgct ggcatcggta aagaagattt
    11401 gccgcaactg caaaatcatc cggcgcaacc ggaccgtgcg ggtgatctgc aaggatccgc
    11461 gccataagca gcgccaaggc tgaggctgga agcgctatgg gttttcaggc tataatcgcg
    11521 agtttcgcgt tttcggggta gaacatggcc cgcatcgcag gcgtcaacat tcctaatcat
    11581 cagcatgccg aaatcgccat cacggcgatc tacggaatcg gccgcgcgcg cgccaaggcg
    11641 atctgcgccg ccgccaacgt ggccggctcc accaagatca aagacctgag cgacgcggac
    11701 atggagcgtc tgcgcgagca ggttgcgaaa ttcaccgtcg aaggtgatct gcgccgcgaa
    11761 gtgtcgatga acatcaagcg gctcatggat ctgggctgct accgcggttt gcgtcatcgc
    11821 cgcggtctgc ccgtgcgcgg tcagcgcacc cgcaccaatg cccgcactcg caagggaccg
    11881 cgcaaggccg cgatcgccgg caaggcgcaa ccgggcaagt aaacggtatc agagcgcgtg
    11941 agcactcact aaggattcga gtaaatggct aaaccgggac aaaccgcatc gcgagtgcgg
    12001 aagaaggcga agaagaacgt tgccgaaggc atcgcgcacg ttcacgcctc gttcaacaac
    12061 accatcatca ccatcaccga tcgccagggc aatgccctgt cgtgggcgac ttcgggcggt
    12121 gccggcttca agggttcgcg taagtcgacg cctttcgccg cacaggtggc cgccgagcag
    12181 gcgggccgca tggcccagga atgtggggtg aagaacctcg aagtgcgcat caagggcccc
    12241 ggtcccgggc gcgaatctgc cgtgcgcgcg ctgaacgcgc tcggcctgaa aatcaccagc
    12301 atcagcgacg tcacgccggt accccataac ggctgccgtc cgccgaagaa gcgtcgtatt
    12361 taacaggagc agccagagtg gcccgcaacc ttgaccccaa atgccgccag tgccgccgcg
    12421 agggcgagaa actgttcctc aaaggcgaaa agtgcttcac cgacaagtgt gccatcgagc
    12481 gtcgcagcta tccgcccggc cagcatggcc agaagcagag ccgcccgtcc gattacgcca
    12541 atcagctgcg cgccaagcag aagattcgcc gcatctacgg cgtgctcgaa tcgcagttcc
    12601 acagaaccta caccgaggcc gcgcgtcgca agggtgtgac cggcgagacg ctgctgcagt
    12661 tgctggagtc gcgcctggat tccgtcgcct accgtatggg cttcggttcc tcgcgcaccg
    12721 aagcgcggca ggtcgtgcgc cacaacggcg tgctggtcaa cggcaagcga gtgaacattc
    12781 cgtcctacca ggttcgtccc ggtgacgtga tcgaagtggc cgaaaaggcc aaagcgcagc
    12841 tgcgcgtgaa aggcgccgcc gaagcggcgg agtcgcgcgg tttccccgag tggatcgaag
    12901 tggacatcaa ggcgctgaaa ggcacgttca aggccgtgcc gcagcgttcg gaactgccga
    12961 cgacgatcaa cgagaatctg gtgatcgaac tttattcgaa gtaattgcgt gtctgacgca
    13021 atcgaatcga tccactaaca atacgaggac aaggaaaccg catgcaaagc acaagcttcc
    13081 tcaagccgcg aatcatcgac gtgcaggcca tcgcgcccta tcacgcgaaa gtggtgatgg
    13141 agcctttcga gcgcgggtac ggccacacgc tgggcaatgc gctgcgccgt gtgctgctgt
    13201 cttcgatgcc cggctttgcg cctaccgagg tgcagattgc cggcgtgttg cacgaatact
    13261 cgaccctcga aggcgttcaa gaagacgtcg tcgatatcct gctgaacctc aagggcgtgg
    13321 tgctgaaact gcacagcggc tccgaggcga tgctgcgcct gaacaaggaa ggcgaaggcc
    13381 cggtcacggc agcggacatc gaagtcacgc atgacgtgga agtgatcaac ccggaacacg
    13441 tcatcgccca tctggcgccc ggcggcaagc tggaaatgtc gatcaaggtg gagcgcggcc
    13501 gcggctatca gcccggcaac gtccgtctcg tgccggaaga gagcaaaggc atcggccgca
    13561 tcatcctgga cgcctcgttc agcccggtgc gccgagtgag ctatcaggtc gaatcggccc
    13621 gcgtcgaaca gcgtaccgac ctcgacaagc tgatcatgga catcgagacc aacggcgtga
    13681 tcgagccgga agaagcgatc cgctacgccg cgcgcgtgct ggtcgatcag ttgtccgtgt
    13741 tcgcggcgct ggaaggcacg cctgtcacgg tggataccga acgcaaggcc ccgcaggtcg
    13801 atccgatcct gctgcggccg gtggacgatc tggaattgac cgtccggtcg gccaactgcc
    13861 tgaaggcgga aaacatctat tacatcggcg acctgattca gcgtaccgaa accgaattgt
    13921 tgaagacgcc gaacctgggc cgcaagtcgc tcaacgagat caaggaagtg cttgcttccc
    13981 gtggtctgac gctgggcatg aaactggaga actggccgcc ggcaggactg gagaccgcgc
    14041 gcggctaagc cgcccgcgag accgcgtccg cgcgatcgag gaaatcaaaa atgcgtcacc
    14101 gtcacggact tagaaaactc aaccgcacca gcgcccaccg tctggccatg ctgcggaaca
    14161 tgaccaactc gctgctcaag cacgaggtca tcaagaccac gctgccgaag gcgaaggaac
    14221 tgcgtaaggt cgtcgagccg atgatcacgc tgggcaagaa gccgacgctt gccaaccgcc
    14281 gcctggcttt cgaccgcctg cgcgatcgcg acatggtgat caagctgttc gacgaactgg
    14341 gcccgcgcta cgccgcgcgc aacggcggct atctgcgcat tctcaagtac ggcttccgcg
    14401 atggggacaa cgcacccatg gcgctggtcg aactggtgga tcgccccgag ccgactgaag
    14461 ccgagtcgga gcaggcagcc gcttaaggcg cgttgcggcg gatactaacg tcgcccaaga
    14521 agagatcgag gccgggatat tcccggcctt tttctttggc tttggcgtgg catggcagag
    14581 ccgtatgacg ggcgcgggcg caaaaaaagg ccggatcatc atatgaatcc ggcctttttc
    14641 gtttgggggc cgccgtgcga tttcggcgga gctctcgtat ttcgcgcggc gccttgctgc
    14701 tcaggatctt gttgccctgg cagtgcgggc gcttacgcgg cgttagcgac gccatcgatc
    14761 ttggtgcgcg aaacgccttg gcgcactttg gaagcggcaa aggtcagcgc tttgttcaga
    14821 ccgttgtcga tggtgaggct caggccgtaa agcaatacga gaatgctggc gacttgcagc
    14881 atcgcaacaa taagttcaac gaggacagtc atttttaatc tccttttcgg gcggcaccat
    14941 tgccgccacg catgcattgt ctcccgtcct ctgctgcatt gcaaaatgat ttattgcatc
    15001 gcaccataag taaggtctat attgcttact tatggctaat atgtcagata aaccattata
    15061 aatcaatgtt attcagcata cgaatggcta tagcagcaaa ataaatgacg attgctccgt
    15121 aataaatctg ttatgaaata agtggctcga catgtataag cttataccag cacctcaacg
    15181 cagcagaatg acccggaaat cattgacgtt ggtgcgggtc gggccggtga tgatgaggtc
    15241 gcccagggca tcgaaaaagc tgtaggcatc gttcgtcgcc gccagttgtt tggcacgcag
    15301 gcccttctgc tccgcgcgct ggagggtatc cggactcagc accgcgccgg cgttgtcttc
    15361 ggagccgtcg atgccgtcgg tgtcggcggc cagggcgtac acctccggca ggctgtccag
    15421 gtcaatggcc aaagacagca aaaattcgct gcagcgcccg ccgcggccgt tcccgcgcac
    15481 ggtgaccgtg cattccccgc cggaaatcag tgccacgggg cgcttgaagg ggtcgccgta
    15541 ggtgtcgacc tggcgggcga gggcgccgta gaccttggcg acttcgctgg cctctccggt
    15601 gacgctatcg cccaggatga ccggcgtaat gccgtgctga cggaagaatt cggcgccggc
    15661 ctgcagcgat tgctgtgcgg tggcgatgac gcgattctcg acgcggtcga aaatcgggtc
    15721 gccgggcttg ggcgtttcgg gaatctcgcc acgcgcgccg cgctccagat aatccaccac
    15781 ggcggccggc gcattgacgc tgtagcgcgc gaagatgtcg agcgcgtcct gatacgtcgt
    15841 cggatcgggc gcgcaagggc cggaagcgat gtgggtgggg tcgtcgccgg tgacgtcgga
    15901 caccaccagc gccagcacgc gcgccttggt ggcggcggcc agacgcccgc cctgcactac
    15961 ggacaggtgc ttgcgcacgg tattcatgtc ctggatgggc gcgccgcaac gcagcagctc
    16021 cttggtcgta gccttcagct catccatggt gatgccttcc accgggagcg acagcaggct
    16081 ggaaccgccg ccggacacca gcgcgagcag cagatcatcg gggcccagtc ccttcaccag
    16141 acgcaggatt tcgcgcgcgg ccttctcgcc gctttcgtcg ggcaccggat ggcccgcctc
    16201 gatgaccttg atgcgattgg tcagcaggcc gtggcggtaa cgcgtgatca ccagcccttc
    16261 cagcggcgcg ctggccggcc agtgctgctc gaccgccagc gccatcgacg cggctgcttt
    16321 gcccgcaccg atcaccaggg tgcggccctt gggcggttcg ggcagatgcg ccggcaggat
    16381 cttgagcggg tcggcggccg ccacggccgc attgaaactg tcgataagga gttggcgagg
    16441 attcatgggg ttctgatcgt gcgattgggc gcgtggggca acgaagccgg agcgcggatt
    16501 ggaacggcgc gaacgccgga aaagcggagc ttgcgcggcc ggcgcgcaac tcctcgtcaa
    16561 ggcgctgaat tatgcaagaa gcaacccgtc cctgccataa gccggggcag gtcgcaggcg
    16621 cgttggcggg tcaggcgcgc agaatggccc aggcgagcag cgcccagccg atgatccatg
    16681 tgataccgcc gaagggcgtg atcatgccca acggccgcac gccgctgaat gcgagggcat
    16741 agaggctgcc gctgaacagg aggatgccga gcgccatggc atagcccgac cagcgcagcg
    16801 gcgccgaggc gggcaggtgg aaggcgagcg cgccgatggc cagcagcgcc agcgcatgcc
    16861 agaactgata ctgcaccgcc gtctgataaa cgctcagcag atcgggcgcg agcttgccgc
    16921 gcagcgcgtg cgcaccgaag gcgccgagca tgacgcccgt catgccggcg aaggcgccga
    16981 aaaggagaaa gaacttggtc gtgccgggca tggtcgcctc gaatgcgaaa cccggacgat
    17041 tgtgcgggtg ctcgagcgcg acggcaagcc gcagcggcgc gcggaggatg gcacaatggg
    17101 ccgtgcgcgg ccgcacgcgc cgcatccatc cgaagcaatg catatggaat acaacctact
    17161 tcccggcacc gatctgcgcg tttcgcgcat ctgtctgggc accatgacct ggggcgagca
    17221 gacggatgca ggcgatgcgc atgctcaact cgatctcgcc gtcgcgcgcg ggatcaattt
    17281 catcgacacc gcggagatgt accccatccc gccgtgtgcc gagacccagg gccgcacgga
    17341 acgcattttc ggcgactggc tggcgcgcca acagcgcgac cggctcatcg tcgcaagcaa
    17401 ggtcgccggt ccgggtcggc gcgaatggct gcgcggcggg cgcaccgagc tcaccaaggc
    17461 caatatcgcc gaagcgatcg acggtacgct caagcgcctg cgtaccgact acatcgacct
    17521 ctaccagatc cactggcccg atcgcgccgt acccatgttc ggcaagacca tgttcgtgcc
    17581 gcgcgaagaa ccgcaggcca cgccgatcat cgagcaggtc gaggcgatgg aggcgctggt
    17641 gcgcgccggc aagatccgct attgggggct gtcgaacgaa acgacgtggg gcgtgtgcga
    17701 atacgtgcgc gtcgcgcgcc tgcacgggtt gccgcagccg gtcacggtac agaatgttta
    17761 caacctcgta tcgcgcaagt tcgacgagga actggccgaa gcctgccatc gagaaaagct
    17821 cgcactgctc gcctacagtc cgctcgcggg cggcgcgttg acgggcaagt accgcaacaa
    17881 cgccttgccc gaaggcgcgc gctatacgct ctttcccgat ttcggcacgc gctttcgcaa
    17941 gccgatggtc gcgccggcga tcgatgccta tcacgacatc gctgccagcc gcggcatgac
    18001 gctcacgcaa ctggccatcg gcttcgcgct gtcgcgctgg cacacgggcg cggcgatcat
    18061 cggtgcgtcc ggactcgcgc aactcgaaga gaacctggcc tacgccgatg cgccgccgct
    18121 cgacgaagcg acgctcgccg agatcgacgc cgtgcacgag cgctatccga gcccggcgca
    18181 ctgagtcatg acggaagccg ccacactgct cgttctgctc gcgcttgtgg catggtattg
    18241 gtacgacagc atgcgggcgc gtgaactggc gctgcaactc ggtcgcgcgg cctgtgcgcg
    18301 cgacagtctg caactgctcg acgacacggt gcagtgcgtg cgcctgcgct tcgcgcgcgg
    18361 cgaggacggc cgcctaaagc tgaagcgcac ctatcgcttc gagttcagcg acaccggcac
    18421 gaatcggcgc gacggcagca tcgtgttgca cggcacggcg gtcgaatcgc tctacatgga
    18481 gccctttctg gtgcagtagc cgacgattga agactaaagc tcatgggcaa atggattgaa
    18541 ggcaccgtaa tcggccagcg gcagtggacc gaccgcctgt actcgctgca ggtgaaggcc
    18601 gacctgccga gtttcgaagc cggccagttc atcaaacttg cgctcgcggt caacggcgag
    18661 atggtcgcgc ggccctattc ctttgtgaac gcgcccaagg aacggcctta cgagttctat
    18721 tacatcgtcg tgcccgaagg tccgctgtcg ccggcgctga cgaaactcga accgctcgac
    18781 aacatctatt tcgccgccca accctccggc tttctggtgc tgtcggaagt gcccgactgc
    18841 gatcatttgt ggatgctgtc caccggcacg ggtatcggcc cctttctgtc gattctcaag
    18901 accgaggcgc cgtggaagcg tttcaaaaag atcgtgctgg tccatgccgt gcgccacggt
    18961 gcggagttga cctatcgcga ccagatcgag ccgctgctgg aggcgtatcc cgggcaactg
    19021 caactgattt ccttcgtcag ccgcgaggag cgtcccggag cgctgacggg gcgcattccc
    19081 gaagccatca ccgacggccg gctggaaaaa gcggccggac tcaaactcgc accggaggac
    19141 acgcacgtca tgatgtgcgg aaacccggag atgatctcgg ccgtcacggc gctgctcaag
    19201 gcgcgcggca tgaagaagca ccggcggcgc gaccggggcc atatcaccgt cgaaaactac
    19261 tggtagctcg gcgttccggc agcaatggac gttgagagcg gggaggaggc gtgaactggc
    19321 agcaggcgct tgacctctat tgcgagcgta ccgacgcgag cttttggtcc gagccgctca
    19381 atgcgctcag caatgcggcc tttctcgtcg cggccgtctt gctcgcccgt cgcgcgcgtg
    19441 ccgacggcgg ccgagcaccg cgggacatcg acgcgctcgc ggtgctgatc gcgctcatcg
    19501 gcttcggcag tttcaccttc cacaccgtcg cgaccgtctg ggcctccctg ctcgacgtgc
    19561 tgttcatcca gatctacatc tacgtctatc tcgcgcgcta tttgcggcgt atcgtcggcg
    19621 tgaacctcgt ctggatgtac ggcgggctgg tgctttactg gctgttcgag caggcagtga
    19681 ccggcggctt cgagccgggc gcgttgaacg gctcctatcg ctatctgccc gcgctcctcg
    19741 cattggcggg catgacgctc tatgcgcggc gcgtgcatgt tcccgctgcc gcgccgctcg
    19801 ccgtggcggt cggcctgctg tgcgtctcga tcgtactgcg gaccatcgat ctcggcgtct
    19861 gtcacctcaa tccgctcggc acgcatttct tctggcatct gatcaatgcc gccgtgctgt
    19921 actacgccgc ggtcgcgctg ctgcgcagtg aagcggccgg tcggtacccc cggccgatat
    19981 aatcctccgc tcgccctctc gggcgtgtgg attcataagg cgctgagtgg aagccgcaaa
    20041 gaagaacgtc gcggtgcttg ccgcgctgca ggcactgctg ttcaccaaca attcgaccgc
    20101 catcgccctc aacggcctgg tcggttacgc actcgccgac aacaaggcgc tggcgacgct
    20161 gccggtgacg ggctgggtgg tgggcgcggc cctgaccacc tacctcgctt ccatgctgat
    20221 gcgccgcatc ggacggcgcg caggcttcac ggtcggcacc tgcatcggca tcgtcggcgc
    20281 ctcgctcgcc tgtctggcga tttacctcgg caacttctgg ctgttctgca gcggcaccgt
    20341 ggtcttcggt gtgtacaacg ccttcggtca gtattaccgc ttcgccgcgg ccgatgcggc
    20401 gcccggcgaa ttcaagagcc gcgccatctc gctggtgctc gcgggtgggc tggtcggcgg
    20461 catcatcggg ccgaccacca gtcagtggac caaggatctg ctgcctgtcg cctatctggg
    20521 tgcgtatctc acgctggtcg tttttctgct ggtggtgctc gtcgttctgc gactgctgga
    20581 catcccgccg cctgccgcag ccgtgcgaca ggacagcggc cgaccgctcc ggcaactggc
    20641 ggcgcagccg gctttcatgg ttgcggtact ggccggcgcc gtgggctacg gcgtgatgaa
    20701 tctgctgatg accgccacgc ctctggcgat ggatctctgc ggtcattcct acggtgccgc
    20761 ggcgaatgtc atttcctggc acgtcatcgg tatgttcggg ccatccttct ttaccggctc
    20821 gctgattgcg cgctacggcg tgctgcgcat tctgctgatc ggcgtggcgc tcatgcttgt
    20881 ttgcatcgcc gtcgcgctgg ccggcatcgc ggttgccaac ttctggctgg cgctggtgct
    20941 gttgggcgtc ggctggaatt ttctatacat cggcggcacc acactgctca ccgaaaccta
    21001 tcgccccgcc gagcaggcca aggcgcaggg cgcgaacgac atgctgatct tcgtcaccat
    21061 ggcggtatcc tctttttcct ccggcatgct gctggaggtg aacggctggc agatgctgaa
    21121 ctatctggcc attccctttg tcgtcgcggt cggcgtcgcc gtcctatggc tcatggcttt
    21181 gcgccgtcgc accgccccgc tggcaacatg aagcaaaagc tcgatcccca ggttgcggcc
    21241 ttgctggagc gcatggcgcg caccggcacg ccgccttaca gacaactcgg tgtggatgcc
    21301 gcgcgcgagc tctatcgcat gacgcgcttt accgtcggag tgccgcataa agaaccggca
    21361 cagaccgagg acctcgcggc cgaagggccg gccggcgcga ttcccttgcg tctttatcgg
    21421 cccgccaaag cgggcgcgga cgaagtgctg ccctgcgtcg tctatttcca cggcggcggc
    21481 tgggtgctgg gtgatctgtc cacgcacgac gaagtgtgtc gcgggctcac caacctcgcg
    21541 cattgcgccg tggtggcggt ggattaccgg cttgcgcccg aacacaagtt tcccgcagcg
    21601 gtggaagacg cgatcgccgc gacgcagtgg atcgtcgaag cagccggacg cttgcgcatc
    21661 gatgcgtcgc gcgttgcggt tgcgggcgat tcggcgggcg gcacgctcgc cacagtgacg
    21721 gcaatcgccg cgcgcgatgc gggcggcccg ccgctggtca tgcaggcgtt gatctatccc
    21781 tccaccgaca tgaccgaggg caccgagtcg cacgagcttt atgccgaagg ctacggcctc
    21841 acgcgcgaga acatcctgtg gttccgcgag cactatctct cctgcgaggc ggatgcgtgc
    21901 gactggcgtg cctcgccgct gctcacgcgc gatctgagcg ggttgccgcc ggcctatatc
    21961 ctcaccgccg gattcgatcc gctgcgggac gaggcctggc agtacgcgca gcgtttgcaa
    22021 gaagcgggcg tcagagtgac ctacgaatgc ttcgaaggca tgatccacgg cttcatcatc
    22081 atgggcgggg cgatcgccgc tgcgcgtcac gccatttatc gcgtcgcgca ggctttgcgg
    22141 ccggcgctga gcaccgcacc cgttcgtctg tagcgaggag gaggcgtcgt ggccattcgc
    22201 gcaatgaatc atttcaccgt gctgagcgcc gacctggacg ccacgcgcgt tttctattgc
    22261 gatctgctcg gcttgcgtgt gggcccgcga ccgccgctcg gtttccccgg tctgtggctt
    22321 tacgcgggcg atcaggcggt gctgcatgtc atcggcggtc gcgagttgcc ggcgcagcgc
    22381 agtggcgtca tcgatcacat ggcgttcacg gccgagaacc tggcgcaaat cgtcgatgcg
    22441 ctgcaggcgc gcgacatcaa gtacagcctg cgccggatgc cgcaggaagg cggctggcaa
    22501 ttgttctgcc gcgacccgga gggtgcgttg gtggaactgg atttcgctgc cgaagagccg
    22561 gcgccgaccg gccacgcttg aaaggccgcc gctagggccg tatcggccgc accgcgtacg
    22621 gccttgcgcg ttcgaagtgc tgcaattgcg agcggctttc gcgctccatg tgctcgcgtt
    22681 cgaggcgttg ggtcgcgtgc gtccccggcg catccgggcg catgcgtagc gcgtcgagcc
    22741 ggcgttgctg tcggctgtgc aagctgtcct gctcgcgccg ctgttgcgcg tgcaggctcc
    22801 gcagagcgga ctgctcctcg gcgctcgatc cgcgtgcgcg caactccgcc gccgcctgcc
    22861 ggttgcgcag ggcaacactg tcggactgct gctggtggat gagcagattg cgctcgatcg
    22921 ggccgcgatc gacggcgtcc gccggcggcg ccgcggcgca aagcagcagt gcgcaaagcg
    22981 gcggcgacaa tcgcttccag gctgaggaaa ggcggttcat gttcctccga ctgcgtgctg
    23041 tgccggtcgt tccctcctgt gcactggtat catcggccgc ctgacccaga gcagttccgt
    23101 gttgtccgtc attcttcttc tttccactct ttccttgccg gtccgacgcc catgagcctg
    23161 cgtcgggtca agcgcgcgct cggcctgcgc gccagtgtcg aagtcagcga gaagcgcggc
    23221 gtgcggacgc tgcatctggg tggtgacgcg atccagagcg cgattcgcct gtccgcgccc
    23281 gatgagctgg agctttccta cacgcgcgcg atgatgagct ggctgctctt tcagcccaaa
    23341 ccgcgcgaag tgctgatgat cggcctgggc ggcggttcgc tggcgcgctt cgttcacgag
    23401 cgcatgcccg acgtgcgcat caccgccgtg gagatcaacg agcaggtggt ggccgccgcg
    23461 cacagctttt tcggcctgcc tttcgaagac aagcggctga aagtgaaaat cgccgacggc
    23521 ggcaagtatg tgcccgcgca tcgcaacgcc tgcgatgtgc tgctgctcga cgccttcgac
    23581 gacggacgct cggtgcccgc gctggccacc caggcgtact acgacgcgtg ccgcgcctgc
    23641 ctgcggcccg gcggcgtgct ggtggtgaac ttcatccgcg acgagccgcg cttcgacgcc
    23701 tatctctcgc gcatcgagcg cagcttcgac ggcaaagtgc tgctgctgcc ctgcgaggac
    23761 cgggtcaaca acatcgtgct ggccttcaag gacgggccgg tgcggctggc catcgatccg
    23821 ctcaagcgcg ccgcacgggc gttgaagaag cgctaccgcc tgcccttcga cgcctacgtg
    23881 cgcgacctgc tctactacaa cggcaagacc gccgccttcc tgcgcatcgg cctggagcgt
    23941 tagcgcttca ccagttccac ccgtcggttt ttcgcgcggc ccgcttcgcc gtcgttgctg
    24001 gcgatgggcg ccgccatgcc gacaccgact gccgtgaggc gatcccgcgc aatcttgtgc
    24061 tgcttcacca gtgcgtcgac caccgcctgt gcgcgccgct ccgacagcga acggttgtaa
    24121 tccagcgccc ccacgttgtc ggtgtggccg acgatcagga ctttcagttt gggctgcgtc
    24181 gacagcagct tggcgatttc gctcagggcg ccagcggatg ccggtttgag ctcagccttg
    24241 tcggtatcga agtagatctc gtacagcgcg atgtgacctt cggcatccag gcccttcgcc
    24301 atggcgttcg catcgacttt caccataccc tgttgcatgg cctggctttc gacgatctgc
    24361 aggttggcgt agacgcgatc cttgttcggg ccgccgatgg aataggcacg aacgacgtac
    24421 accgccgcgt acacatcgcc ggtcggacgg gcgagcctgg ccagcagata gcgctgatcc
    24481 ttttcgttgt acgccatgaa ggtgtgcaga tccgccggcg tcgccgcttc gttgaagggg
    24541 cggccgccgc acacctcgtt gtcgcagcgg tacagcagtt cgaagccgcc ggcctgtaga
    24601 gcctgctcgt aattgcggaa gacctccagc gcactgcgcc cgctgggcgc gtggtaggcg
    24661 atgcgcgtga acttgccttc cacggtccgg tacgcatccg gattcctatc gcgtccgccg
    24721 cgctgcttga tcggtccgcc gatcaggtgc acttcgtcga aggcgcgctg ttcgtatttg
    24781 atgatttccg aaccctggta gcgagacacc atggggtggt ccttggcgcc gggcagatcg
    24841 gcggcccaca ggccggtggc ggcgcacagc aggaagatca tgcagcagag gcgtgcagcg
    24901 aggcgtgcca tggacaactc cggtcggatg aaggtgcgcc attctatgcc atgagcatga
    24961 agggcgtttt tcgccccggc cggccgccgc ttgcaacccg cgccgctgct aaaatcgccg
    25021 gccatggacg cactacaaca agcccgtatc gatcttgccg tggcgctgcg ctgggctgcg
    25081 cgcctggacc tctccgaagg ggtctgcaat cacttcagct tggccgcgcc cggcatgccc
    25141 ggccatttcc tcatcaaccc gcaggggttc cactggtcgg aaatcacccc gcgccacatc
    25201 ctcgtcgtgg atgtgaaggg caaggtggtg gaaggcgatt acaccgtgga acccggcgcg
    25261 ttctggatcc acagccgcat ccacaactcc gagcgcgcgc acaaatgcat catgcacacg
    25321 catatgccct atgcgacggc gctgtgcatc accgagggcg gccgcctgga accggccagc
    25381 cagaatgcgc tgcgctacta cggccggatc gcctacgacg aggagtacaa cggtctggtg
    25441 acgagcgagg aactgggtga tcgcttccta cagaaaatgg gcgatgcgga aatcgtgttc
    25501 ctcgccaacc acggcgtcat cgtctgcggc gataccgtgg ccgcggcgtt cgaggatctc
    25561 tactacctcg agcgcgcctg catgctgcag gtcatcgcgc aaagcacggg caagccgctc
    25621 aagcggatct cgcacaaggt cgcgtcggag acggtgaaac tgatggtcga ggaccgcgaa
    25681 caggccgagg ccttcatcgc cgcctgcaag cg
//
LOCUS       DATGOU010000113         2877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_778827, whole genome shotgun sequence.
ACCESSION   DATGOU010000113 DATGOU010000000
VERSION     DATGOU010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778827"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VKZ48_06775"
     CDS             complement(<1..914)
                     /locus_tag="VKZ48_06775"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLU77586.1"
                     /translation="MNAPTSRILVVGGGIAGLATALKLAPLPVTLLVKAPLGTEASTP
                     WAQGGIAAAMGADDSPRLHAVDTLAAAAGVGDAHIASRVTGEAPACIEDLLRYGVPFD
                     RDEADRLALGLEAAHSRRRIVHAGGDATGRVVIDTLIRAVQAANHIDVIDNVRVTELL
                     TDDGSVRGVQGERAGAPFFLAANAVVLATGGAGGLYTHTTNPLGSIGSGFVVAARAGA
                     VLRDVEFVQFHPTAMAVGRDPMPLATEALRGEGATLINQHGERFMLDVPGRELASRDV
                     VARAIWRQIEQGNEVYLDARECVGARFPT"
     gene            complement(1039..2070)
                     /gene="nadA"
                     /locus_tag="VKZ48_06780"
     CDS             complement(1039..2070)
                     /gene="nadA"
                     /locus_tag="VKZ48_06780"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HLU77587.1"
                     /translation="MTATATMPSTTLRYTERYTPEVAERTAHLYERVRSVVPELLWPK
                     YAPDVDAIMRLKHEKNAVILAHNYQTPEIFHLVADFVGDSLALAREAAKCDADVIVQA
                     GVHFMAETSKLLSPDKKVLIPDLRAGCSLADSITGADVRLLREKYPGVPIVTYVNTSA
                     EVKAESDICCTSANAVKVVESLGVPRVIMIPDEFLAQNVAAQTKVDIITWAGHCEVHE
                     LFTVEDIERFRLLHPGVVVLAHPECPPEVVQAADFAGSTSGMINYVGEKRPARVVLIT
                     ECSMSDNVAVQFPEVEFVRPCQRCPHMKQITLENIRHALETMTHEVTVDPELAPRARR
                     AIERMLEVK"
     gene            complement(2241..>2877)
                     /locus_tag="VKZ48_06785"
     CDS             complement(2241..>2877)
                     /locus_tag="VKZ48_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77588.1"
                     /translation="QSAGVPEAEWRNWYRYFPWEDWRDGRPAVLDRIAEGLRIWCAAA
                     DDAKLRRQRERRVRWTFGNRKLPWNDEPVLERYELLYEAGLVPEAHRDGRRTPCRPLE
                     EGEPMVADHRRILATGIARLRGKIKYRPVVFELMPDTFPLLHLQRTVEALAGVRLHKP
                     NFRRLVEGQGLVEDTGAVTTETGGRPARLVRYRSDAVAGMSTGFRLPVARR"
BASE COUNT          494 a          967 c          908 g          508 t
ORIGIN      
        1 gtggggaaac gcgcacccac gcattcgcgc gcgtcgagat agacctcgtt gccctgttcg
       61 atctgccgcc agatggcgcg agccaccacg tcgcgcgacg ccaattcgcg tcccggcaca
      121 tcgagcatga agcgttcgcc atgctggttg atcagtgtcg caccctcgcc gcgcaaggct
      181 tcggtagcca acggcatggg gtcgcggccg accgccatgg cggtgggatg aaattgcacg
      241 aactcaacgt cgcgcagcac cgcgcctgcg cgcgcggcga ccacgaaacc gctgccgatc
      301 gagccgagcg gattggtcgt gtgcgtataa agcccgcccg caccgccggt ggcgagcacc
      361 acggcgttcg ccgccagaaa gaacggcgcg ccggcgcgtt cgccttgcac gccccgcacg
      421 ctgccgtcgt cggtcagcag ttcggtgacg cgcacattgt cgatcacatc gatatggtta
      481 gccgcctgca cggcgcggat cagggtgtca atcaccacgc ggccggtcgc gtcgccgccg
      541 gcgtgcacga tgcgccgacg cgaatgcgcc gcctccaggc ccagcgccag gcggtccgcc
      601 tcatcgcgat cgaacggcac accgtagcgc agcagatctt cgatgcacgc cggcgcctcg
      661 ccggtcacgc gcgaagcgat gtgcgcgtcg cctacgcccg ccgcagcggc caacgtatcg
      721 acggcatgga ggcgcggtga atcgtccgcg cccatcgcag ccgcgatgcc gccctgtgcc
      781 cacggcgtgg aagcttccgt accgagcggc gccttgacca gcaaagtcac cggcaagggc
      841 gctagcttga gcgccgtcgc cagaccggcg atgccgccgc cgacaaccag gatgcgtgag
      901 gtaggagcgt tcatcgtcgc ctttaccgtg tgctgtgtct cgcttccgcg gaaatgaaat
      961 gatcgcgcgc tgcgcgcata ccgaacgtgt ggatccccgc ctgcgcgggg atgaaatgac
     1021 tctgcgctac gcgcatcgtc atttcacttc cagcatgcgt tcgatggcac ggcgggcgcg
     1081 gggcgccagt tcgggatcga ccgtcacttc gtgcgtcatc gtctccagcg cgtgacgaat
     1141 gttttccagc gtgatctgct tcatgtgcgg gcagcgctgg caaggccgca cgaattcgac
     1201 ttccggaaat tgcacggcga cgttgtcgct catcgagcat tcggtgatca gcactacgcg
     1261 cgccggacgc ttctcgccca cgtagttgat cattccggag gtagagccgg caaaatccgc
     1321 ggcctgcaca acttcgggcg ggcactccgg atgggcgagc accaccacgc ccggatgcag
     1381 caggcggaag cgctcgatgt cctccaccgt gaaaagctcg tgcacttcgc aatggccggc
     1441 ccaggtgatg atgtccacct tggtttgcgc ggcgacgttc tgcgccagga actcgtcggg
     1501 gatcatgatg acccgcggca cgcccaggct ttcgacgacc ttcaccgcgt tggccgaggt
     1561 gcagcagatg tcgctttccg ccttcacttc ggcggaggtg ttgacgtagg tgacgatcgg
     1621 cacgcccggg tatttttcac gcagcaggcg cacgtccgcg ccggtgatcg aatcggccag
     1681 cgaacaaccc gcccgcaggt cgggaatcag caccttcttg tccgggctga gcaacttcga
     1741 ggtttcggcc atgaaatgca cgccggcctg cacgatgacg tccgcatcgc atttggccgc
     1801 ttcgcgcgcc agcgccaggc tgtcgcccac gaaatcggca acgagatgga agatctcggg
     1861 tgtctggtag ttgtgcgcca aaatgaccgc gttcttctcg tgcttcagac gcatgatggc
     1921 atcgacgtcg ggcgcatact tcggccacaa cagttccggg acaacgctgc ggacgcgctc
     1981 atacagatgt gcggtgcgct cggcgacttc gggcgtgtag cgctccgtgt atcgaagcgt
     2041 ggtcgacggc atggtggcgg ttgcagtcat tatgctcacc ttgagtatat tgggacgata
     2101 ataaaaccga gatactctcg gtggtcgtta ttctagtcct gagaataagc ccgtcaagcg
     2161 atttggatca agatttcgag tttttacaaa ttgcgcgccc cgaccggtat ccggaccgct
     2221 atcgtcggtt aaacagcggc ttagcgtcgc gcaaccggta aacggaagcc tgtcgacatg
     2281 cccgcgactg cgtcgctgcg gtatcgcacc agccgcgccg gccgcccgcc ggtctctgtg
     2341 gtcaccgcgc cggtgtcctc caccagcccc tggccttcca ccagccggcg gaagttcggc
     2401 ttgtgcagcc gcacgccggc cagcgcttcg acggtgcgtt gcagatgcag caagggaaag
     2461 gtatccggca tcagttcgaa caccaccggc cggtatttga tcttgccgcg cagccgcgca
     2521 atgccggtgg caagaatgcg ccggtgatcg gcgaccatcg gctcgccctc ttccagcggg
     2581 cggcaaggcg tccgccgtcc gtcgcgatgc gcctcgggca ccaatcccgc ctcgtaaagc
     2641 agctcgtagc gctccagcac cggctcgtcg ttccagggca gtttgcgatt cccgaaagtc
     2701 cagcgcacgc gccgctcgcg ctgacggcgc agcttggcat cgtccgccgc cgcgcaccag
     2761 atcctcaagc cctcggcgat gcggtcgagg accgcggggc gcccgtcccg ccagtcctcc
     2821 catgggaagt agcggtacca attgcgccat tcggcctcgg gcacgccggc cgactgc
//
LOCUS       DATGOU010000114        13061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_780029, whole genome shotgun sequence.
ACCESSION   DATGOU010000114 DATGOU010000000
VERSION     DATGOU010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13061
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_780029"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VKZ48_06790"
     CDS             <1..603
                     /locus_tag="VKZ48_06790"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HLU77589.1"
                     /translation="QDPHPGAVHRRSRHLGRRRFGVPRSVKSAPKQAASTGADGRPWY
                     RQLWPWLILAVPAISILLSIVMLTLALRSDDGLVVDDYYKEGLGINEVLDKKARAAEL
                     GLRAQLTFSEDRRVVRALVHGQSTQGAPLLLRFVHPTRAGEDQSVTLHPLGGMMYEST
                     VAALSAGRWHVVLEDQGETWRLSGIWHTSASQLMLEAPRD"
     gene            618..917
                     /locus_tag="VKZ48_06795"
     CDS             618..917
                     /locus_tag="VKZ48_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77590.1"
                     /translation="MMQRAIWVFWPAFIVAIGAEGVLFTLVDPMSLHIFNEPHHYSRL
                     AVYSVAFFALWLFAATSSALTCFFQRTAAEINRCPLRPVERPVGCPKREESDACC"
     gene            972..1415
                     /locus_tag="VKZ48_06800"
     CDS             972..1415
                     /locus_tag="VKZ48_06800"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLU77591.1"
                     /translation="MFKNVLIPIDRSAVSLKAAKAGIRLAKHLGATVTIYTGVERLER
                     YYGDEGIAASPAAIKTLQKRTRTAAQRVLNDVKAVADKAGVTAKLVIDEPNSPAEGIV
                     NAATKNKCDAIFIGSRGHGAVKSLLLGSVTQRVLATSKLPVLVYR"
     gene            complement(1612..2343)
                     /gene="fnr"
                     /locus_tag="VKZ48_06805"
     CDS             complement(1612..2343)
                     /gene="fnr"
                     /locus_tag="VKZ48_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HLU77592.1"
                     /translation="MNSGKNVSIQQLKTVCSTCSLRELCLPMGLAPEEIDQLDNLVYT
                     RRKIKRGDSLYRAGDPFGSLNAVRTGFFKSTVIHEDGRDQVTGFHMPGEIMGLDGIGT
                     DRHAVSTIALEDSEVCVIPYSRLEEASLKVRSLQYQFHKMMSREIVRDQGVMMLLGTM
                     RAEERLAAFLLNLSQRFGARGYSATEFNLRMTREEIGSYLGLKLETISRIFSKFQDQG
                     LITVQQKHVRILDTKGLRKLLGREC"
     gene            2441..3829
                     /gene="hemN"
                     /locus_tag="VKZ48_06810"
     CDS             2441..3829
                     /gene="hemN"
                     /locus_tag="VKZ48_06810"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HLU77593.1"
                     /translation="MNATDLIPDADLLRRFDRPGPRYTSYPTADRFVDAYDVATQRRV
                     LEAGRQKWGAKPLSLYVHLPFCNTLCYYCGCNKVITKDHSRAAKYLRYLEREMDLVVD
                     IIGARRHVEQLHWGGGTPTFLSEPELRELMDMLRSRFDFAADGEYSIEIDPRSVDVSK
                     MAVLGEIGFNRASLGVQDFDPDVQKAVNRIQSYEVTERAVNAAREHGFKSVNVDLIYG
                     LPKQNPARFSETLSKTLILRPDRIALYNYAHLPTLFKPQRRIHEADLPSPATRIELLQ
                     TAIRTLTDAGYVYIGMDHFASPDDDLAKAQKMGNLHRNFQGYSTRAECDLIAFGVSAI
                     SAIGSTYSQNVKSLDEYYDRLDQNELPVMRGIELNADDVLRRAVIQSLMCQFTLSIES
                     LQTAYLIDFHKYFADEMEALREYVDMGMVEIDDEWITVTPKGRLFVRAIAMVFDRYLR
                     QSEMRARYSKVV"
     gene            3829..4578
                     /locus_tag="VKZ48_06815"
     CDS             3829..4578
                     /locus_tag="VKZ48_06815"
                     /inference="COORDINATES: protein motif:HMM:NF024778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLU77594.1"
                     /translation="MTLALTAFLTGLFSGVHCMAMCGGVVSALSMSRGRESIRIEPDG
                     TASLRDESLAARVPVQLAYSGGRILSYGAAGAVAGGIGGTVLLTQSVLPAQVILAVLA
                     NTLLMLLGLHLAGIGNWVARMERIGAPVWRRLAPLGRRFMPADTPPRAFAVGMLWGWL
                     PCGLVYSTLALALMSGGPADGAIVMLAFGLGTLPNLVAAGLAIERLQPLLRRQGVRRA
                     AAALVFSLGVWGLVRTPGLYETVREGILCLI"
     gene            4624..5055
                     /locus_tag="VKZ48_06820"
     CDS             4624..5055
                     /locus_tag="VKZ48_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLU77595.1"
                     /translation="MYKILLAVDGSDPSVRATQALIKSLREHKASPQIDVLTVHLPLP
                     NIGTIGSVITKEIRDRYYQEECDEALAPTIKLLDEAGIKYQVHRAIGPIADTIVEKAA
                     ELGSDLIYMGTHGRGGATKLIMGSVANKVLQLSPIPVQLVK"
     gene            5061..5267
                     /locus_tag="VKZ48_06825"
     CDS             5061..5267
                     /locus_tag="VKZ48_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HLU77596.1"
                     /translation="MEQTTLQIGGMTCQGCVGSVTRVLQALPGVEQVEVSLARNEAQV
                     RYDPSRVQPEAFRRAIEGAGFDVQ"
     gene            5562..6248
                     /locus_tag="VKZ48_06830"
     CDS             5562..6248
                     /locus_tag="VKZ48_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLU77597.1"
                     /translation="MSRYDTPSLLRQLPLFSVLSEAEIQRVAAATAQLRVSTGARLFS
                     RGDPCAGFHVVVQGRVKLLLETSDGAEKVMEICGPGHSFGEAVMFLQKPYFVAAEVLE
                     PSLLLHIGKGAVMSELDNNPDFAKHMLAGLSMRLHRLVSDLEAFTMRDGTHRVAAYLL
                     SEHGDSNSGSFEIRLPVSKGVLASRLHITREHFSRILNELSTAGLVHVQGPVIRVLDA
                     VKLRQHVNAN"
     gene            6310..6816
                     /locus_tag="VKZ48_06835"
     CDS             6310..6816
                     /locus_tag="VKZ48_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family protein"
                     /protein_id="HLU77598.1"
                     /translation="MRAIRTAVGLCCVIALIMPFGAVRAASGHAPADHASHAPTLVRH
                     KPWGTAGEPGNAVRTVVIGMTDDMRFTPDTIDIRRGETVKFVLRNDGRLLHELVLGTE
                     ETNRALYALILKAPDTHHHGPDMIHVEAGRTDTMVWHFNRAGRFEFACLLPGHYSAGM
                     LGVINVTE"
     gene            complement(6843..6995)
                     /locus_tag="VKZ48_06840"
     CDS             complement(6843..6995)
                     /locus_tag="VKZ48_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77599.1"
                     /translation="MRRVMAGRRLTVLLVVLLVVLLLVMLMMVILMRALFGPRSRYKA
                     EAQHTG"
     gene            7002..7220
                     /locus_tag="VKZ48_06845"
     CDS             7002..7220
                     /locus_tag="VKZ48_06845"
                     /inference="COORDINATES: protein motif:HMM:NF023036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-binding protein"
                     /protein_id="HLU77600.1"
                     /translation="MAEGEVLEVDRTTRRITLQHGDLPHLGMGPMSMTFRVQDDALLE
                     RIKVGDKVRFSAARVGGVMSVTAIEAIE"
     gene            complement(7279..7779)
                     /locus_tag="VKZ48_06850"
     CDS             complement(7279..7779)
                     /locus_tag="VKZ48_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLU77601.1"
                     /translation="MLKVLVPVNGSECSRAAVDEALRIARANGDAMIQLVNVQPRMPY
                     HVSRFLSGGEISALRAERGARALEGARQRVEAAGVRCAAHVLRGHIVQRISTFAAETG
                     TDQIVIGTSPARGRLFRESIADRLIEESPVPVDVVRGGPIGMFERFGLPAGLGLGLAM
                     FWYSNQ"
     gene            complement(7900..8616)
                     /locus_tag="VKZ48_06855"
     CDS             complement(7900..8616)
                     /locus_tag="VKZ48_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HLU77602.1"
                     /translation="MEFFSMEFFSALAAIVLIDLVLAGDNAIVIALAARSLPHHLQTK
                     AIFWGTFGAIALRSAMTVFVVWLLDIPGLMLAGGLLLVWIAWKLIAPANDEGHENIKP
                     ATTFWAAIKTIVIADAVMGIDNVLGVAGAAHGSFLMVVLGLLISIPIIVFGSKLVLKL
                     MERFPVIIYLGAGVLAYTSAKMITEEPLIQEFLVLDGAIVGAIYVLLLGGIVGGGYLA
                     NRRHTQVVEHQPQHVSGSFE"
     gene            9139..10098
                     /locus_tag="VKZ48_06860"
     CDS             9139..10098
                     /locus_tag="VKZ48_06860"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLU77603.1"
                     /translation="MLDRRLRRYIRNGTLTQLAAFEAVVQLGSFTRAADALHMAQPTV
                     SLLVKKLSETLGVQLLNTTTHGIQVTEAGAEAYKFCQEFFQSFAGLDDRLDEMRLTTA
                     GVLRIAVCTGAESTVPAMLCHYCMQNPQVRLHLSVANRAHLLERLAAGADDFYVFGVP
                     PEDDEVQMHPLCTEEFHIYASASHPHARRRRLRFADIANETFVMREVGARSRLVVDAL
                     FAEHECRPNVRMEMDNNEAIICAVATGFGIALLSQYAVGPNPRHAMLTPLRVEGLPIR
                     HQWQLVYRPNKKLSDAEQQLVEQLCSNPVVESSRSVLHDAMRI"
     gene            complement(10116..10478)
                     /gene="arsC"
                     /locus_tag="VKZ48_06865"
     CDS             complement(10116..10478)
                     /gene="arsC"
                     /locus_tag="VKZ48_06865"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003120014.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HLU77604.1"
                     /translation="MSESITIYHNPACSISVAALAMIRERGVEPQIIEYLKTPPSREK
                     LTELIAAMGIKPRDLLRRKEPRYHELDLDNTKWTDEELIGFMVENPILMNRPIVVTSR
                     GTRLGRPSEAAIVDILPG"
     gene            complement(10529..12565)
                     /locus_tag="VKZ48_06870"
     CDS             complement(10529..12565)
                     /locus_tag="VKZ48_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77605.1"
                     /translation="MAEAEDVLHDVARHATVYVRDLWRRNRPAPVRPVTALDDVAQRL
                     DLLIAAIFRRSFALRIAIPPAPRNALRRFYERGQGPQPTQAVPATDDRSIWLPRELDM
                     PDREAATERYRVFALQQAMRAVRGSARGIPADEDALVRACYQLCEAAAADVALVQLLP
                     GLASGIGTLRREALTQRPPLDTFPARRRPLEALLRELLAHDCLSLPPCWPTLDTPVAS
                     LAHAREIAAALRDDIPDAHRLRGVWLLPDAWTGELRPVAAAASVRDRHPDDEEDDTRD
                     HNPRSARMDRRPAVRDALPDEDEPQDPGAWMIQPGEPLEQAEDPMGMQRPTDRDEKTA
                     AEELADSLSELNEARLVSTPGTPKEILLSDDAPEARVKGGTHAAPTDGDGIEYPEWDY
                     RMAAYREPGARVIVLPPTAGDPAWVEKTLKAHRAMLDAIRRRFELLRAQRMRLRRQRD
                     GEDIDLDAYIESIADYRAGRSLSQRLYQTTRQARRDTAIALLIDISGSTDAWVASHRR
                     VIDVEREALLLVCLALESMGEPYCVQAFSGEGPDCVTVRELKRFDEHYGADVGTRIAA
                     LEPERYTRAGAAIRHAAATLMRKPASHRLLMLLSDGKPNDMDEYDGRYGVEDMRQAVT
                     EVKLQGISPFCLTIDRQGASYLPQVFGVGNYALLMQPAELPSALIDWIRRLVAH"
     gene            complement(12739..>13061)
                     /locus_tag="VKZ48_06875"
     CDS             complement(12739..>13061)
                     /locus_tag="VKZ48_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ C-terminal domain-containing
                     protein"
                     /protein_id="HLU77606.1"
                     /translation="PSTRQRFIALDFDFLPPEQEARIVVHESGVDNAVAVQLCALAQR
                     LRALRDRGLAEVPSTRLLVATARLIAGGVAVHQACEAGLIAPLSDDPTLVAAMRDLVA
                     ASFV"
BASE COUNT         2453 a         4041 c         4146 g         2421 t
ORIGIN      
        1 caagatcctc atccaggtgc agtccatcga cgatcccggc atctcggccg tcgaagattc
       61 ggtgttcctc gttcggtgaa atccgctccc aaacaggccg catccaccgg cgccgacggc
      121 cgtccgtggt atcgccagct ctggccctgg ctgattcttg ccgtccccgc gatctcgatt
      181 ctgctgagca tcgtcatgct cacgcttgcg ctgcgctcgg acgacgggct ggtggtggac
      241 gactattaca aggaggggct gggcatcaat gaggtgctgg acaagaaggc ccgcgccgcc
      301 gaactcggcc tgcgcgccca attgacgttc agcgaagatc gccgtgtcgt gcgtgcactt
      361 gtgcacggac aatcaacgca aggcgctcca cttttgctac gctttgtcca tcccacccgg
      421 gcgggggagg atcagagcgt cacactccac cccctcggcg ggatgatgta cgaaagcacc
      481 gtcgccgctt tgtcggcagg tcgctggcat gtcgtcctcg aggatcaggg cgaaacctgg
      541 cggctcagtg gcatctggca tacgagcgcg tcgcaactga tgttggaggc gccgcgcgac
      601 tagttggagg aactaggatg atgcagcgtg cgatctgggt attctggccg gcctttatcg
      661 tagcgatcgg cgccgaaggg gttttgttca cgctggtcga cccgatgtcc ctccacatat
      721 tcaacgagcc gcatcactac agtcgacttg cggtctattc cgttgcattc tttgcgttgt
      781 ggctgtttgc cgcgacgtcc agcgcattga cctgtttttt tcagcgcacc gccgcggaaa
      841 tcaatcgctg ccccttgagg ccggtcgagc gaccggtcgg ctgcccaaag cgggaggaaa
      901 gcgatgcgtg ttgctaggat tcccgatctt tgtgctcaac accaataacc cataaccaag
      961 gagagagtgg aatgttcaag aacgtcctga tcccgatcga tcggtccgcc gtatcgctca
     1021 aggccgccaa ggcgggaatc cggttggcca agcacctggg cgcgacggtg acgatctata
     1081 ccggcgtcga gcgcctggaa cgctactacg gcgacgaagg tattgcggcc agtcccgcgg
     1141 cgatcaagac actgcagaag cgtacccgca cggcggcaca gcgcgtcctg aacgacgtca
     1201 aggcggttgc cgacaaggca ggcgtgacgg ccaagctggt gatcgacgag ccgaactcgc
     1261 cggcggaggg aatcgtcaac gccgcgacca agaacaagtg cgacgccatc ttcatcggct
     1321 cgcgcggtca cggcgcggtg aagtcgctgc tgctgggcag cgtcacgcag cgtgtgctgg
     1381 caacctcgaa gctgccggtg ctggtgtatc gctaaaaggc agtggagagc gaagagtcga
     1441 gagtggagag taaagccggc tctcgaaact gaactctccc gcagccggaa tccggaaaga
     1501 caaaagcctc ggaccgcaag gttcgaggct tttttaaccg ttcatgggga gcgtccgcgt
     1561 agcgggcagt cgcactatcg acgctcagtt cccggctttg gaccgtagcc tctaacactc
     1621 ccgcccgagc agcttgcgca agcctttggt atcgaggatg cgcacatgct tctgctgcac
     1681 ggtgatcagg ccctggtcct ggaatttgga gaagatgcgg ctgatggttt ccagtttcag
     1741 ccccagatag ctcccgattt cctcgcgcgt catgcgcaga ttgaactcgg tcgcggagta
     1801 gccgcgcgcg ccgaagcgct gcgagaggtt gagtagaaaa gccgccaggc gctcttcggc
     1861 gcgcatggtg cccagcagca tcatgacgcc ctgatcgcgc acgatttcgc ggctcatcat
     1921 cttgtggaac tgatactgga ggctgcgcac cttgagcgag gcttcctcca gccgggagta
     1981 ggggatgacg cagacttcgc tgtcttccag cgcgatggtg ctgacggcgt ggcggtcggt
     2041 gccgatgccg tccaggccca tgatttcgcc gggcatgtga aagcccgtga cctgatcgcg
     2101 cccgtcctcg tgaatgaccg tgctcttgaa gaaaccggtg cgtacggcat taagggaacc
     2161 gaacgggtcg ccggcgcgat agaggctgtc gccgcgcttg atcttgcgtc gggtgtagac
     2221 gagattatcg agctggtcga tctcttcggg ggccagcccc atgggcagac aaagctcgcg
     2281 cagactgcat gtcgaacaga cggtcttgag ctgttgaatg ctgacgtttt ttccgctgtt
     2341 catggcaaag ccattctaac ctgtggtaga gctttgatcc acatcaatca acgatttacg
     2401 gcttggtcca cactgctttc atagagtttc gaaggctgcc atgaacgcta ccgacctgat
     2461 ccccgatgca gacctgttgc gccgcttcga ccggccgggt ccacgctaca cctcctaccc
     2521 gaccgccgac cgcttcgtcg atgcctacga cgtcgcgacg cagcgccggg tgctggaagc
     2581 ggggcgccag aaatggggcg ccaagccact ttccctttac gtgcacctgc cgttctgcaa
     2641 caccctgtgc tactactgcg ggtgcaacaa ggttatcacc aaggaccaca gccgcgccgc
     2701 caagtacctc cgctacctgg agcgggaaat ggacctcgtc gtcgatatta tcggggcgcg
     2761 tcggcatgtc gaacagttgc actggggcgg cggtacgccg acctttctca gcgagcccga
     2821 actgcgtgag ctcatggaca tgctgcgcag ccgcttcgac ttcgccgccg atggcgaata
     2881 ttccatcgaa atcgacccgc gcagcgtgga cgtttcgaaa atggccgtgc tcggcgagat
     2941 cggtttcaac cgcgcctcgc taggcgtgca ggatttcgac cccgacgtgc aaaaggcggt
     3001 caaccgcatc cagagctacg aagtgaccga aagggcggtg aacgcggcgc gtgagcacgg
     3061 cttcaaatcg gtcaacgtcg acctgatcta cggcctgccg aaacagaatc cggcccgctt
     3121 cagcgagact ttgtcgaaga cgctgatcct ccgtccggac cggatcgcgc tgtacaatta
     3181 cgcccatctg ccgacgctgt tcaaaccgca gcgacgcatc cacgaggcgg acctgcccag
     3241 cccggccacg cgcatcgagc tgttgcagac cgccatccgt acactgaccg acgccggcta
     3301 cgtctacatc ggcatggacc atttcgccag ccccgacgac gacctcgcca aggcgcagaa
     3361 gatgggcaac ctgcaccgca atttccaggg ctattcgacg cgcgccgagt gtgatctcat
     3421 tgccttcggc gtgtcggcca tcagcgcgat cggctcgacc tacagccaga acgtgaaatc
     3481 gctggacgaa tactacgacc ggctggacca gaatgaactg ccggtcatgc gtggcatcga
     3541 actgaatgcc gacgacgtgc tgcgccgcgc agtgatccag tcgctcatgt gccagttcac
     3601 gctgagcatc gaatcgctgc agaccgccta cctgatcgat ttccacaagt acttcgccga
     3661 cgagatggaa gcgctgcgcg aatacgtgga catgggcatg gtcgaaattg acgacgagtg
     3721 gatcaccgtc acgcccaagg gtcgcttgtt cgtgcgcgcc attgcgatgg tcttcgatcg
     3781 ttacctgcgt cagtcggaga tgcgcgcgcg ctactccaag gttgtttgat gacgcttgcc
     3841 ctcaccgctt tcctcaccgg cctgttcagc ggcgtgcact gcatggcgat gtgcgggggg
     3901 gtggtgagcg cgctcagcat gtcgcgcggc cgcgaaagca tccgcatcga accggacggc
     3961 actgccagcc tgcgcgatga gagtctggcc gcccgggtgc cggtgcaact ggcctacagt
     4021 ggcggccgca tcctgagcta cggggcggct ggtgccgtcg ccggcgggat cggcggcacg
     4081 gtgctgctga cgcaaagcgt gctgcccgcg caggtcatct tggccgttct ggccaatacc
     4141 ctgctgatgc tgctcggcct gcacctggcg ggaatcggca actgggtggc ccgcatggag
     4201 cgaatcggtg cgccggtctg gcgacgtctt gccccgctcg gccgccgctt catgccggcc
     4261 gatacgccgc cgcgcgcctt cgcggtgggc atgctgtggg gctggctgcc ctgcggactg
     4321 gtatacagta cgctggcact ggcgctgatg agcggcgggc cggccgacgg cgcgatcgtc
     4381 atgctggcct tcggtctggg cacgctgccg aatctggtgg cggcgggctt ggccatcgaa
     4441 cgtctgcagc cgttgctgcg ccgccagggt gtccggcggg cggcggcggc gctggttttt
     4501 tcactcggcg tatgggggct ggttcgtacg ccgggactgt acgaaacggt gcgcgagggc
     4561 attctttgcc ttatttagcg cgcgctcctc gcggcgcgtg gcgacaataa aaatggagct
     4621 gcaatgtaca aaatcctact cgcggtggac ggatccgatc cttcggtgcg tgccactcaa
     4681 gccctgatca aatcgctgcg cgagcacaag gcctcgccgc agatcgacgt gctgaccgtg
     4741 cacttgccgt tgccgaacat cggcaccatc ggctcggtga tcaccaagga aatccgcgac
     4801 cgctattacc aggaagagtg cgacgaggcg ctggcgccca ccatcaagct gctggacgag
     4861 gcggggatca agtatcaggt ccatcgcgcc atcggcccca tcgccgacac catcgtcgaa
     4921 aaggcggccg agctgggcag cgacctgatc tacatgggga cgcacggccg cggcggcgcg
     4981 accaagctca tcatgggctc ggtggcgaac aaggtgctgc agctttcgcc cattccggtg
     5041 caactcgtca agtaagcgtc atggaacaga ccacactgca aatcggcggc atgacctgcc
     5101 agggttgcgt cgggagcgtc acgcgagtgc tccaggcgct gcccggcgtg gagcaggtcg
     5161 aggtatcgct ggcgcgcaac gaggcgcagg tgcggtacga tccgtcgcgc gtgcagcccg
     5221 aagcctttcg ccgggcaatc gaaggcgccg gtttcgacgt gcagtgaggc aagatcgcca
     5281 gccgggcggt ttttgcccgt ctggcgatcg catcagcaag tccctcgggg gcaggccccc
     5341 tataaatccc cgaagcatcg atggcagcgg cgattttgcg cggaccgcag ggaacacctg
     5401 ccagcgcggg cggtccaatc gcgcgacccg tgccggcctt tgcggccgaa gactgcacgc
     5461 aggagaaaaa gcgatccgca agtgtttgac gccgcctccg gcaaacaaac aagcttgatc
     5521 aggatcaaat aggtatttgc aatgggaggc atgatcgccg catgagccgc tacgacacac
     5581 catccctgct acgacagcta ccgctgttca gcgtcctgtc ggaagccgaa attcaacgcg
     5641 ttgccgcagc gaccgcacaa ctgcgcgtgt ccacaggcgc gcgcctgttc agccgaggcg
     5701 atccgtgcgc cggtttccat gtggtggtgc aggggcgcgt caagctcctg ctcgaaacca
     5761 gcgacggtgc cgagaaagtc atggagatct gcgggccggg gcacagtttc ggtgaagccg
     5821 tgatgttcct gcagaagccc tatttcgtcg ccgcggaagt gttggagcct tcgctgctgc
     5881 tgcacatcgg caagggcgcc gtcatgtccg aactcgacaa caatccggat ttcgccaagc
     5941 acatgctggc cgggctgtcg atgcgactgc accggctggt gagcgacctg gaagctttca
     6001 ccatgcgcga cggcacgcat cgcgttgcag cgtatctgct gtcggagcac ggcgattcga
     6061 attcgggcag tttcgagatc cgcctgccgg tcagcaaggg cgttctcgct tcgcgcctgc
     6121 atatcacgcg cgagcacttc tcgcgtatcc tcaacgaact gtccaccgcc ggcttggtgc
     6181 atgtccaggg accggtcatt cgcgtcctgg atgccgtcaa gctgcgccag cacgtcaacg
     6241 ccaactgagc gggcgcgcca tcgcaggatg gcgcgcgctt cgcaacgccc cgaaccttcc
     6301 gatcacaaaa tgcgcgcaat cagaacggcg gtcggtctgt gctgcgtcat cgcattgatc
     6361 atgccgttcg gtgccgttcg ggccgcgagc ggccatgcgc ccgctgatca cgcctcacac
     6421 gcgccgaccc tcgtccgaca caaaccctgg ggcactgcgg gggagccggg aaatgcggtt
     6481 cgcaccgtcg tcatcggcat gaccgacgac atgcgattca cgccggatac gatcgacatc
     6541 cggcggggcg agacggtgaa gttcgtgctg cgcaacgacg gccggttgct gcacgaactg
     6601 gtgctcggaa cggaggagac gaaccgggcg ctgtatgcgc tcattctcaa agcgcccgat
     6661 acgcatcacc atggcccgga catgatccat gtcgaagcgg gtcgaaccga cacgatggtg
     6721 tggcacttca atcgagcagg tcgcttcgaa ttcgcgtgcc tgctgccggg acattactcc
     6781 gcaggcatgc tgggcgtcat caacgttacg gaatagagaa atcgatgcga aagttccatt
     6841 ggtcagccgg tgtgctgagc ctctgccttg tatcggctgc gtgggccgaa cagggcgcgc
     6901 atgagtatca ccatcatcag catcaccagc aacagcacca ccaacagcac caccagcaac
     6961 accgtcaatc gacggccggc catgacgcgg cgcatgcggc gatggccgaa ggcgaagtgc
     7021 tcgaggtcga tcgcacgacg cgccgcatta cgcttcagca cggcgatctg ccgcatctgg
     7081 gtatggggcc gatgagcatg accttccgcg tgcaggacga cgcactgctc gaacgcatca
     7141 aggtagggga caaagtgcgc ttcagtgcgg cgcgcgtcgg tggcgtgatg agcgtcacgg
     7201 ccatcgaagc gatcgaataa agcggtgcgg acagggcggc gcgcccgcgc gcatgaagcg
     7261 cgggcgtgtc gcacttcatt attggttcga gtaccagaac atcgccaggc cgaggcccag
     7321 gccggccggc agaccgaaac gctcgaacat gccgatcgga ccgccgcgta ccacgtcgac
     7381 cggcaccggc gactcctcga tcagccggtc ggcgatcgat tcgcggaaca gccggccgcg
     7441 tgccggcgag gtgccgatta cgatctgatc cgttcccgtt tccgcggcga aggtcgaaat
     7501 acgctggacg atgtgtccgc gcagcacatg ggccgcgcac cggacaccgg ccgcttccac
     7561 acgttgccgc gccccttcga gtgcacgggc gccgcgttcg gcgcgcaagg cgctaatctc
     7621 gccgccggac agaaagcggg aaacatggta cggcatgcgc ggctgcacat tcaccaactg
     7681 aatcatcgcg tcgccgttgg cgcgggcaat gcgaagcgct tcatcgaccg ccgcgcgcga
     7741 acattcggaa ccattgacgg gaaccagaac tttcaacatg acttgctcct tatgcgttgc
     7801 tgcttcagga aagcatgcag atcgacgagc ttgcacgcac ccgtaatgcg gttgtgatgc
     7861 atcgggtcga tccgcgtctg catgcgctta cgacctcgat cattcgaaac tgccgctgac
     7921 gtgttgcggt tgatgttcca cgacttgcgt gtgacgccgg ttggcaaggt agccaccgcc
     7981 gacgatgccg ccgaggagca gcacatagat ggcgcctacg atcgcaccgt ccaacacgag
     8041 gaactcctgg atcaggggtt cctcggtgat catcttcgcc gaggtatatg ccagcacacc
     8101 ggcgcccaga taaatgatga ccggaaagcg ctccatcaac ttgaggacca gcttcgagcc
     8161 gaaaacgatg atcggaatgc tgatcagcaa gccgagcacc accatcagaa agctgccgtg
     8221 cgcagcaccc gcgacgccca gtacgttatc gatgcccatt acggcgtcgg cgatgacgat
     8281 ggttttgatt gcagcccaga aagtcgtcgc cggtttgatg ttttcgtgac cttcgtcgtt
     8341 tgccggtgcg atcaacttcc atgcgatcca gaccagcaac aatccgccgg ccagcatcag
     8401 acccggaata tcgagcagcc agacgacgaa caccgtcatg gcggagcgaa gcgcgatcgc
     8461 gccgaaggtg ccccagaaga tcgcttttgt ctgaaggtgg tggggcaggc tgcgtgcggc
     8521 caatgcgatg acgattgcgt tatcgcctgc gagaaccagg tcgataagca cgatggcggc
     8581 gagcgccgag aaaaactcca tgctgaagaa ttccatcact ctctcgttcg gcgcggatag
     8641 acgtcacaac cgaatcaggc gtgtccgcga gcctttacct gattccggca aaggtctcgc
     8701 tctcgctaag tgcgacggat gaccggagac ggcagtctcg cgatgacgat catcacgcag
     8761 gaattgcttc ccggaactac tcccccttgt gggtcttcga acaacagtgc gttcaaattg
     8821 cgttcatcct gccgaacgag agagagggag agaaatgaag tctcgcaatg aattcattgc
     8881 gatacgaaat aatcggcccg ctcgttttgc ctacacttgc ggtcatagcg cggtctcgcg
     8941 ctatactggg cgtgcagtca taagtaacag ggtcgtcatc ccggcgatgc attcgtaatt
     9001 gcccggccct gttctgcagc gacgatgctc cattgtcctg caagcgagac ctttgcgaag
     9061 tttgccctaa agccaatttc gttcagcgcc cgacccattg ggcgttattc gcttgctgtg
     9121 ttgttggagg atcatgcgat gctcgaccgg cgtttacggc gatacatcag aaacggcact
     9181 ctgactcaac tggccgcttt cgaagccgtc gtgcagctgg gcagcttcac tcgcgcggca
     9241 gatgcactgc atatggcgca gccgaccgtt tccctgctgg tgaagaagct gagcgagacg
     9301 ttgggcgtgc agttgctcaa cactacgaca cacggcatcc aggtgaccga ggccggcgcc
     9361 gaagcataca aattttgcca ggagttcttt caatccttcg cgggcctgga tgatcggctg
     9421 gacgagatgc gtctgacgac ggccggcgtg ctgcgcattg ccgtatgtac cggagccgaa
     9481 tcgaccgtgc ccgccatgct atgtcactac tgcatgcaga atccgcaagt ccggctgcat
     9541 ctgtcggtgg ccaatcgtgc gcatttgctc gaacggctgg cggcgggcgc ggacgacttc
     9601 tatgtattcg gggtaccgcc cgaggacgac gaagtgcaga tgcatccgct atgcacggaa
     9661 gagtttcata tctatgcgtc cgcgagtcat cctcacgcac ggcggcgcag gctgcgcttc
     9721 gcggatatcg cgaacgaaac ctttgtgatg cgagaagtcg gcgcacgttc gcgactggtg
     9781 gtcgatgcgc tgttcgccga gcatgagtgc cggccgaacg tacgcatgga aatggacaat
     9841 aacgaggcga taatctgcgc agtggcaacg ggtttcggca tcgccttgct gtcgcagtat
     9901 gcggtcggcc cgaacccgcg tcacgccatg ctcacgccgc ttcgcgtcga ggggttgccg
     9961 atccggcacc agtggcaatt ggtttaccgt cccaacaaaa agctcagcga tgccgaacag
    10021 caactggtcg agcaactgtg cagcaatccg gtcgtggagt cctcgcgatc ggtgctgcac
    10081 gatgcgatga gaatctagga tggcgcgccg gcgattcagc ccggcagaat atcgacgatc
    10141 gcggcttcgc tgggacgacc caaacgcgta ccgcgcgaag tgacgacgat agggcggttc
    10201 atcaggatgg ggttctcgac catgaagccg atgagttcct catcggtcca cttagtgttg
    10261 tccagatcga gttcgtgata gcgcggctcc ttgcggcgca gcaggtcgcg cggcttgatg
    10321 cccatggctg caatcagttc cgtcagtttt tcgcggctgg gcggcgtctt gagatattcg
    10381 atgatctgcg gctctacgcc ccgttcgcga atcatcgcca gtgcggcgac cgaaatgctg
    10441 caagcggggt tgtgatagat ggtgatcgat tcactcataa cttctcctcg ttgatggatg
    10501 gaaagaagcg gaacgcgtgc gcgccgcctt agtgtgccac cagacgcctg atccagtcga
    10561 tcaatgcgct cggcaactcg gcgggttgca tgagcagcgc gtaattgccc acgccgaaaa
    10621 cctgcggcag atagctcgcg ccctgacgat cgatggtgag gcagaagggc gaaatgccct
    10681 gcagcttcac ttcggtgacg gcctgacgca tatcctcgac gccgtagcgg ccgtcgtact
    10741 catccatgtc gttcggcttg ccgtcggaca gcagcatgag cagacgatgg ctggccggct
    10801 tgcgcatcaa tgtcgcggcc gcgtgacgga tggcggcgcc tgcgcgcgta tagcgttccg
    10861 gttccagcgc cgcgatgcgc gtgccgacgt ccgcgccgta gtgctcgtcg aatcgcttga
    10921 gttcacgtac cgtcacgcag tcggggcctt cgccggagaa tgcctgcacg caataaggct
    10981 cgcccatgct ctccagcgca aggcatacaa gcagcagtgc ttcgcgttcc acgtcgatga
    11041 cgcgccgatg cgaggcgacc caggcgtcgg tggagccgct gatgtcgatg agcaacgcaa
    11101 tggccgtatc gcgtcgtgcc tgtcgcgtcg tctgataaag ccgctgggat agcgagcgcc
    11161 cggcgcggta atccgcgatg ctttcgatat aggcgtccaa atcgatgtct tcgccgtcgc
    11221 gctgtcgcct cagccgcatg cgttgcgcgc gcagcaattc gaagcgccgc cggatcgcat
    11281 cgagcatggc gcggtgcgcc ttcaaagtct tctccaccca ggcgggatcg cctgcggtag
    11341 gcggcagcac aatgacgcgc gcgcccggtt cccggtaggc ggccatgcgg tagtcccact
    11401 ccggatattc gatgccgtcc ccgtccgtcg gcgcggcgtg cgtgccgcct ttgacccgcg
    11461 cttcgggcgc gtcgtcggaa agcaggattt ccttgggtgt gccgggggtg ctgaccaggc
    11521 gcgcttcgtt caactccgac agcgagtcgg caagttcctc cgccgccgtc ttctcgtcac
    11581 gatcggtggg gcgctgcatg cccatgggat cttccgcctg ctcgagcggt tcgcccggct
    11641 gaatcatcca cgcgcccgga tcctgcggct cgtcttcgtc cggcagcgcg tcgcgcacgg
    11701 cggggcgtcg atccatgcgt gcgctgcgcg ggttgtgatc gcgcgtatcg tcttcctcgt
    11761 cgtcggggtg ccggtcgcgc acgctcgccg cagcggcgac cgggcgcagt tcgcccgtcc
    11821 aggcatccgg caacagccac acaccacgca agcgatgcgc atcgggtatg tcgtcgcgca
    11881 gggcagccgc gatttctcgt gcatgcgcga gcgaggcgac gggcgtatcg agcgtcggcc
    11941 agcacggggg caggctgaga cagtcgtgag cgagcagttc gcgcagcaac gcttccaaag
    12001 gacgtcgtcg cgccggaaac gtatcgaggg gcggccgttg ggtcagcgct tcacgccgca
    12061 gcgtaccgat gccggacgca aggccgggca gcagttgcac cagcgcgacg tcggcggcag
    12121 ccgcttcgca gagttggtag caggcgcgga ccagggcgtc ctcgtcggcg ggaatgccgc
    12181 gcgcactgcc acgcacggcg cgcatggcct gctgcaaggc gaacacgcgg tagcgttcgg
    12241 tcgctgcttc acggtcgggc atgtccagtt cgcgcggcaa ccagatcgac cggtcatcgg
    12301 tcgccggaac cgcctgcgtg ggctgcggcc cctgaccgcg ttcgtagaag cggcgcaagg
    12361 cgttgcgcgg cgccggcgga atggcgatac gcagtgcaaa gctacgacgg aagatggccg
    12421 cgatcagcag gtcgagccgc tgtgcgacat cgtccagcgc cgtgaccggc cggacgggcg
    12481 ccggacgatt gcgccgccac agatcgcgta cataaacggt ggcatggcgc gcgacatcgt
    12541 ggagtacgtc ttcggcttcg gccatggtgg ttggggagag agtgaagggt cgagagtgat
    12601 ctgggcccgt tcacccttcg actcgctcgc tgggctcgcg gctcagggcg aacggcaagt
    12661 agtggtttac gttcgtggcg agcttgtcga accatgagcg gaaaccactt aaccggagtt
    12721 ccttaactct cctgccgttc aaacaaaact cgccgccacc agatcgcgca tggcggcaac
    12781 cagtgtgggg tcgtccgata agggcgcaat caacccggct tcgcaggcct gatgcacggc
    12841 aacgccgccc gcgatgaggc gcgcggtggc gacaagcaat cgcgtgctgg gtacttcggc
    12901 gaggccgcgg tcgcgcaacg cgcgcaggcg ctgggcgagg gcgcacagtt gcactgccac
    12961 cgcgttatcg acaccgcttt catgcacgac gatgcgcgct tcctgttcgg gcggcagaaa
    13021 gtcgaagtcg agcgcgatga accgttggcg cgtgctcggc t
//
LOCUS       DATGOU010000115         8083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_2134348, whole genome shotgun sequence.
ACCESSION   DATGOU010000115 DATGOU010000000
VERSION     DATGOU010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8083
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2134348"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2063
                     /locus_tag="VKZ48_06880"
     CDS             <1..2063
                     /locus_tag="VKZ48_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLU77607.1"
                     /translation="EREQNILYTAFVERPKHLDPVQSYTEDEATIIGQIYEPPLQYHY
                     LKRPYELVPLTATDVPKASYLDAAGRPVDAESPEVAFSVYELRIRSGILFQPHPAFAQ
                     DEAGRYRYHELSREAIARKWTLDDFPETGTRELTAADYVYQIKRLAHPHVHSPIYGLM
                     SQYIAGLTDFAVTLREAQPDERGWLDLRKYALEGAEVVDRYTYRIKLKGRYPQFVYWL
                     AMNFFAPIPWEADRFYSQPGMTEGRNLSLDWYPVGTGPYMLVQNNPNARMELRRNPHF
                     RGEAYPADGEPGDEAAGLLRDAGKRMPFIDRIVFTREKESIPYWNKFLQGYYDASGIS
                     SDSFDQAVQIGAEGQADLTPEMRERGIQLSTSVATSTFYLAFNWLDPVVGGDSEHARK
                     LRQAISIAVDWEEFISIFMNDRGIAGQGPVPPGIFGFREGAEGVNPVVYDWRDGAPVR
                     KPIEAARQLLAEAGYPGGRDARTGQPLVLYLDTTDRGPGDKSRLDWYRKQFAKLNIQL
                     EIRTTDWNRFQEKIRSGNTQLFFLGWNADYPDPENFLFLFYGPQSRAKTQGENAANYS
                     NPEYDALFVKVKSMENSPERQALIDRMVEILRHDAPWSFGFHPKSYSLYHAWLGNVKP
                     NQMARNGMKYRRVDPELRQARREEWNRPHVWPLVAIAVLLTVLTLPAVIGYRRRERAV
                     ARAA"
     gene            2060..3037
                     /locus_tag="VKZ48_06885"
     CDS             2060..3037
                     /locus_tag="VKZ48_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLU77608.1"
                     /translation="MIAYIVRRVLYAIPILIGVNLITFALFFVVNTPDDMARMQLGAK
                     RVTTEAIEKWKADRGYDKPLLYNEAADGLGKLTGTIFYEKSVRLFVFDFGVADDGRDI
                     GHEIATRMWPSLAIAVPVFLIGLAVNISFALMMAFFRGTYLDFSGVVLCVAMMSISSL
                     FYIIGGQYLISKLWHLVPISGYGGSFDAARFVVLPVVVGVISGIGSGGRWYRTIFLEE
                     IGRDYVRTARSKGLSETLVMYRHVLKNGMIPILTGAVVVIPLLFMGSLIVESFFGIPG
                     LGSYTIDAIQAQDFAVVRAMVFLGSVLYIVGLLLTDLSYTLVDPRVRLE"
     gene            3059..4492
                     /locus_tag="VKZ48_06890"
     CDS             3059..4492
                     /locus_tag="VKZ48_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLU77609.1"
                     /translation="MWVLLWTDALLWLLVFAGVIYALHVHGQPHLRAPWRRVARSAPG
                     MSALVFLIVFIAIALLDSIHFRPQLASASDTAETVYSVEVVSVLDRLLEPLRERGERT
                     YSAPLAVRSYAKETVEGLDGTVRREFPRLMHGGAHLPDESDRARDVRSRTLQGLGWGA
                     LVWLLFPLFALRRVARQTKQGPGGALHDLLRGHGDFPWRSVLASLLALCLISGVLVTL
                     AQGYHVLGTDKVGTDVLYQSLKSIRTGVVIGGLTTLVMLPLAILLGIMAGYFKGWVDD
                     VVQYIYTTLNSIPGVLLIAAAVLMMQVYIDTNPDLFPTTAERADLRLLFLCIILGLTS
                     WTGLARLLRGEALKLRELEYIQAARAFGVRNGRIITRHVLPNVMHIVLIALVMDFSGL
                     VLAEAVLSYIGVGVDPTMISFGTMINAARLEMAREPMVWWTLAAAFAFMFALVLSANL
                     FADAVRDAFDPRVSIRPRMALTARTGR"
     gene            4489..6621
                     /locus_tag="VKZ48_06895"
     CDS             4489..6621
                     /locus_tag="VKZ48_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLU77610.1"
                     /translation="MNESPPTAVADSPQQRMQPASAQPNDVLLRVRDLRTALYTDTAV
                     VRAIDGIDLDIRRGETFALVGESGCGKSMTALSIARLLPENGRVEGGSVELEGVDLLG
                     LSEAHMRDVRGRRIAMIFQEPATSLNPVLTVGRQIEEVITRHTSLRGEAARRRIIELL
                     DAVGIADSARRIDEYPFQLSGGMKQRVMIAVALAADPDLLIADEPTTALDVTIQAQVL
                     ELLKSLQRERGMAILLITHDLGVVSGVADRVAVMYAGQIVETAKRDDFFRAPQHPYSQ
                     KLFAALPTSAKRGDTLDMISGQVPPLSTQFVGCRFADRCPHAWDRCRNEAPGWTPIVA
                     AHAVRCHLREKEPAPPRVADSGGAASFASDTVTTEGLLEVSDLRVHFPIRKGLFKRTV
                     GHVKAVDGVSLRLAPGRTLALVGESGCGKTTVGKAILQLIRPTAGSIRFDGVELTALK
                     GEALRRRRSDFQIVFQDPYASLNPRMRIGDILTEGINALGGASAKQTREARVSELLEQ
                     VGLPEDARYRYPHEFSGGQRQRVAIARALAVRPRLIVCDEPTSALDVSVQAQILNLLR
                     GLQRDYGLAYLFITHNLAVVEYLADEVAVMYLGRIVERGTMEEVLGAPKHPYTRALLA
                     AVPTIEGPREVLRAAGDLPSPVSPPSGCHFHPRCPHAWDKCREAYPDEFTLSDSAGVH
                     CWLYELPSAREFARQAEELKRAMEGDDR"
     gene            complement(6661..>8083)
                     /locus_tag="VKZ48_06900"
     CDS             complement(6661..>8083)
                     /locus_tag="VKZ48_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HLU77611.1"
                     /translation="AILTVALTCAPAHAVDPILHSLPPEYFDIRLPFEEGEARHPALG
                     MQSDSAEDVDVRDVRDLGARRAGDLADNVPDDLWERIRRGFAMPDLDGRLVVERQAWY
                     AARPEQMRVLVERSRRYLYHIVEELEKRGMPTELALLPMVESAFNPMAHSSARASGLW
                     QFIPSTGKYYNLEQNWWYDGRRDIVASTSAALDYLQFLYELHGDWHLALASYNMGENG
                     VLRAMKRNRAKGKPTDYESLRIPKETRYYVPKLQALENIIARPDAFGIELTPIPNAPY
                     FVTVDLTRDIDLNVAAKLAEMPVEELIALNPGNNRPVVSSEVSPQLVLPTDRADVFIS
                     NLAAHKKPLSSWKSYVYKRGDTLAKVAKAHGITVAQLREYNNLRPYAHLRPGQRLLVP
                     VKDRADMAVLPPGLPKIAPAGANQYHIVRRGDTLYEIARKYNLRVADLKRWNGGKSLI
                     RIGQRIKLAPPATQTARKTRSRR"
BASE COUNT         1339 a         2555 c         2653 g         1536 t
ORIGIN      
        1 ccgagcgcga acagaacatc ctctataccg ctttcgtcga acgaccgaag catctcgacc
       61 cggtgcagtc ctataccgag gacgaggcga ccatcatcgg ccagatctac gagccgccgc
      121 tgcagtacca ctacctcaag cggccgtacg aactggtgcc gttgaccgcg acggacgtgc
      181 cgaaggcgag ctatctcgat gccgcgggcc ggccggtgga tgccgaatcg cccgaggtgg
      241 cgttcagcgt ctatgaactg cgtatccggt ccggcatcct gttccagccg catccggctt
      301 tcgctcagga tgaggcgggc cggtaccgct accacgaact atcgcgtgag gcgatcgcgc
      361 ggaagtggac gctggacgat tttccggaga ccggcacgcg cgagctgacg gccgcggact
      421 acgtttatca gatcaagcgc ctcgcgcacc cgcatgtgca ctcgcccatc tacggtttga
      481 tgagccagta cattgccggc ctgacggatt tcgccgtgac gctgcgcgaa gcgcagcccg
      541 atgaacgcgg ctggctggat ttgcgcaagt acgcgctcga aggcgccgaa gtcgtcgatc
      601 gctataccta tcgcatcaag ctgaagggcc gctacccgca gttcgtttac tggctggcga
      661 tgaatttctt cgcgcccatt ccatgggagg cggaccgctt ctatagccag ccgggcatga
      721 ctgaaggccg caacctgagc ctggactggt atccggtagg cacgggcccc tatatgctcg
      781 tgcagaacaa tcccaatgca cgcatggaac ttcgccgcaa tccccacttc cgcggcgaag
      841 cctatcccgc cgatggcgaa ccgggcgatg aagcggccgg gctgctgcgc gatgcgggca
      901 agcgcatgcc cttcatcgac cgcatcgtgt ttacccgcga gaaggaaagc attccttact
      961 ggaacaaatt cctgcagggc tattacgatg cctcgggcat ttccagcgac agtttcgatc
     1021 aggcggtgca gatcggcgcc gaagggcagg ccgatctcac gccggaaatg cgcgaacggg
     1081 gcatccaact cagcacctcg gtcgcgacca gcactttcta tctcgccttc aactggctcg
     1141 atccggtggt cggcggcgac agcgaacacg cacgcaagct gcgtcaggcg atttccattg
     1201 ccgtcgattg ggaagaattc atttccattt tcatgaacga ccgcggcatc gcggggcagg
     1261 ggccggtgcc gccgggtatt ttcggctttc gcgaaggcgc cgaaggcgtc aatccggtgg
     1321 tgtacgactg gcgggacggc gcgccggtac gcaagcccat cgaagcggct cggcagttgc
     1381 tggccgaagc gggctacccc ggaggccgcg atgcgcgcac ggggcagccg ctggtgcttt
     1441 atctggacac caccgatcgc ggcccgggcg acaagtcgcg cctggactgg tatcgcaagc
     1501 agttcgccaa gctgaacatt caactggaaa tccgcaccac cgattggaac cgcttccagg
     1561 aaaagatccg gtccggcaac acgcagttgt tcttcctcgg ctggaatgcc gactatcccg
     1621 atcccgaaaa ctttctcttc ctgttctacg gtccgcaaag ccgcgcgaag acgcagggcg
     1681 agaacgcagc gaactacagc aatcccgaat acgacgcgct gttcgtcaaa gtgaagagca
     1741 tggaaaacag tccggagcgc caggcgctca tcgaccgcat ggtggagatc ctgcgtcacg
     1801 atgcaccatg gtccttcggc ttccatccca agagctacag cctctatcac gcctggctgg
     1861 gcaacgtgaa gccgaaccag atggcgcgta acggcatgaa gtaccgccgc gtggaccctg
     1921 agctgcgtca ggcgcggcgc gaagaatgga accggccgca cgtgtggccg ctggttgcaa
     1981 tcgccgtact gctgaccgtg ctgacgctgc cggcggtgat cggctatcgg cgtcgcgagc
     2041 gcgccgtggc gagggcggca tgatcgccta catcgtccgc cgcgtgctgt acgccattcc
     2101 gatcctcatc ggcgtcaacc tcatcacctt cgcgctgttc ttcgtcgtca acacgccgga
     2161 cgacatggcg cgcatgcaac tgggcgccaa gcgcgtgacg accgaagcga tcgagaagtg
     2221 gaaggccgac cgcggctacg acaagccgct tctctacaac gaggcagccg acgggctcgg
     2281 caagctgacg ggcacgattt tctacgagaa gtcggtgcgg ctgttcgtat tcgatttcgg
     2341 cgtcgcggac gacggccgtg acatcggcca tgaaattgcg acccgcatgt ggcccagcct
     2401 cgccattgcg gtgccggtgt ttctcatcgg tcttgcggtc aacatcagct tcgccctgat
     2461 gatggctttc ttccgcggca cctatctcga tttttccggc gtagtgctgt gcgtggcgat
     2521 gatgtccatc tcctcattgt tctacatcat cggcggccag tacctcatca gcaagctgtg
     2581 gcacctggta cccatttcag gctatggcgg cagcttcgat gcggcgcgct tcgtcgtgct
     2641 gccggtcgtc gtgggcgtga tcagcggcat cggcagcggc gggcgttggt atcgcaccat
     2701 cttcctggag gagatcggcc gggactatgt gcgtacggcg cgcagcaagg gcctgagcga
     2761 aacgctggtg atgtatcgcc acgtgttgaa gaacgggatg atccccatcc tgaccggcgc
     2821 ggtggtggtc attccgctgc tgttcatggg cagcctgatc gtcgaatcct ttttcggcat
     2881 cccggggctg ggcagttaca ccatcgacgc catccaggcg caggacttcg ccgtggtgcg
     2941 cgccatggtg tttctcggtt cggtgctgta catcgtcggt ctgctgctga ccgatctttc
     3001 ctacacgctg gtcgatccac gggtgcggct ggaatgagcg ggcggggcgc ggactgatat
     3061 gtgggtgctg ctgtggaccg atgcgctgct gtggttgctc gtcttcgcgg gcgtcatcta
     3121 tgccttgcac gtacatgggc agccgcacct gcgcgcgccc tggcgccgcg tggcgcgcag
     3181 cgcccccggc atgagtgcgc tggtgttcct catcgtgttc atcgcgatcg cgctgctcga
     3241 ttccatccac tttcgtccgc aactggcatc cgcctccgat actgcggaga cggtctattc
     3301 cgtcgaagtg gtgagcgtgc tcgaccggct gctcgagccg ttgcgtgaac gcggcgagcg
     3361 cacgtattcg gcgccgctcg ccgttcgctc ctatgccaag gagaccgtcg aaggtcttga
     3421 cggtacggtg aggcgcgagt ttccgcgtct gatgcatggc ggtgcgcatc tgcccgacga
     3481 gagcgatcgt gcgcgcgatg tgcgctcgcg cacgctgcaa ggcctcggct ggggcgcgct
     3541 ggtctggctg ctgttcccgt tgtttgcgct gaggcgcgtt gcccgtcaga cgaagcaggg
     3601 gccgggaggg gccttgcacg atctccttcg cggtcacggc gatttcccgt ggcggtccgt
     3661 gttggcgagc ctgcttgcac tctgcctcat cagcggcgtg ctggtgacgc tggcgcaggg
     3721 ctatcacgtg ctgggcaccg acaaggtcgg cacggacgtg ctttaccagt ccctcaagag
     3781 cattcgcacc ggtgtcgtca tcggcggact caccacgctg gtgatgctgc cgctggccat
     3841 cctgctgggc atcatggcgg gctacttcaa gggctgggtg gatgatgtgg tgcagtacat
     3901 ctacaccacg ctcaattcca ttccgggcgt gctgctcatc gccgcagcgg tgctgatgat
     3961 gcaggtctac atcgacacca atccggacct ctttccgacc accgcggagc gcgcggactt
     4021 gcgtttgctg ttcctgtgca tcattctcgg gctgaccagt tggacgggtc tggcgcgatt
     4081 gctgcgcggt gaggcgctga agctgcgcga gctggaatac attcaggccg cgcgcgcatt
     4141 cggcgtgcgc aacgggcgca tcatcacgcg ccatgtgctg ccgaacgtga tgcacatcgt
     4201 gctcatcgcg ctggtgatgg atttcagcgg tctggtgctg gccgaggcgg tgctgtcgta
     4261 catcggcgtc ggcgtcgatc cgaccatgat cagcttcggt accatgatca acgccgcgcg
     4321 tctggaaatg gcgcgcgaac cgatggtctg gtggacgctc gccgcggcat tcgcattcat
     4381 gttcgcgctc gtgctgtccg ccaatctttt tgcggacgcg gtgcgcgacg ccttcgatcc
     4441 gcgcgtctcc atccggccgc ggatggccct cacagccaga acgggacgat gaacgaatct
     4501 ccgcccaccg ccgtcgccga cagcccgcag cagcgcatgc aacctgcgag tgcgcagccg
     4561 aacgatgtgc tgctgcgcgt acgcgatctg cgaaccgcgc tttatacgga tacggcggtg
     4621 gtacgcgcca tcgacggcat cgacctcgac atccgccgcg gcgagacctt cgcactggtg
     4681 ggcgaatccg gctgcggcaa gagcatgacg gcgcttagca tcgcccgcct gctgccggag
     4741 aacggccgtg tcgaaggcgg cagcgtggaa ctggaaggcg tggatctgct cggcttgagc
     4801 gaagcgcaca tgcgcgacgt gcgcggacgg cgcatcgcca tgatcttcca ggagccggcg
     4861 accagcctca atccggtgct gaccgtcggc cggcagatcg aggaagtgat cacgcggcat
     4921 acgtcgctgc gcggcgaggc ggcgcgccgc cgcatcatcg aactgctcga tgccgtcggt
     4981 atcgccgaca gcgcgcggcg catcgacgaa tatcctttcc agctttcggg cggcatgaaa
     5041 cagcgcgtca tgatcgccgt cgcgctggcg gccgaccccg acctgctgat tgccgatgag
     5101 ccgaccaccg cgttggacgt gacgatccag gcgcaggtgc tggaactgct gaagtcgcta
     5161 caacgcgagc gcggcatggc gatcctgctc atcacgcacg atctcggcgt ggtctcgggc
     5221 gtggccgatc gcgtggcggt gatgtacgcg gggcagatcg tggagacggc gaagcgcgac
     5281 gatttcttcc gcgcgccgca gcatccctat tcgcagaagc tgttcgccgc attgcccacc
     5341 tccgccaagc gcggcgacac gctggacatg atctccggcc aggtgccgcc gctgtcgacg
     5401 cagttcgtcg gctgccgctt cgccgaccgt tgtccgcatg cctgggatcg ctgccgcaac
     5461 gaagcgcccg gctggacgcc catcgtggcg gcgcacgccg tgcgctgtca cttgcgcgaa
     5521 aaggagccgg cgcctccgcg ggttgccgac tccggcggtg cagcgtcgtt cgcctcggac
     5581 accgtcacga cggagggctt gctggaagtg agcgatctgc gcgtgcattt cccgatccgc
     5641 aaggggctgt tcaagcgtac ggtgggacat gtcaaagccg tcgacggtgt atctctgcgg
     5701 ctcgccccgg ggcgtacgct cgcgctcgtg ggcgagtcgg gctgcggcaa gacgacggtg
     5761 ggcaaagcca tcctccaact catccggccc accgccgggt cgatccgttt cgacggcgtc
     5821 gaactcacgg cgctgaaggg cgaggccttg cgccgtcgcc gcagcgactt ccagatcgtg
     5881 ttccaggatc cctatgcctc gctcaatccg cgcatgcgca tcggtgacat cctgaccgag
     5941 ggcatcaacg ccctgggcgg cgcgagcgcg aagcagacac gcgaggcgcg cgtgtcggaa
     6001 ctgctcgagc aggtggggct gccggaggat gcgcgctacc gttatccgca cgaattctcc
     6061 ggcgggcagc gtcagcgcgt tgccatcgcc cgcgccctgg cggtgcgacc gcgcctgatc
     6121 gtttgcgatg agccgaccag cgccctcgat gtatcggtgc aggcacagat tctcaatctg
     6181 ttgcgcggtc tgcagcgcga ctacggtctc gcgtatctgt tcatcaccca caacctcgcg
     6241 gtggtggagt atctggccga cgaagtggcg gtgatgtatc tgggtcgcat tgtcgaacgg
     6301 ggcacgatgg aggaggtgct cggcgcaccc aagcatccct atacccgggc gctgctggcc
     6361 gcggtgccga ccatcgaagg gccgcgcgaa gtgttgcgcg cggcgggcga tctgccttca
     6421 cccgtatcgc cgccttcggg ctgtcatttc catccgcgtt gtccgcatgc ctgggacaag
     6481 tgcagggagg cttatccgga cgagttcacc ttgtcggaca gcgccggcgt gcattgctgg
     6541 ctatacgagt tgccgagtgc gcgtgaattc gcccgtcagg cggaggaact caagcgggcc
     6601 atggaagggg acgatcgcta ggccgttcgc ccggggctgc gcgcgaaagt tggcggacgt
     6661 tcagcgtctg cttcgcgtct tccgtgcggt ctgggtggcc ggcggcgcaa gcttgatgcg
     6721 ctgaccgatg cggatgagac tcttgccgcc gttccagcgc ttcagatccg ccacgcgcag
     6781 gttgtacttg cgcgcgattt cataaagcgt gtcgccgcga cgaacgatgt ggtactggtt
     6841 cgcgccggcc ggggcgattt tcggcagacc gggcggcagc accgccatat cggcgcgatc
     6901 cttgacgggc accagcagac gctggcccgg acgcaggtgc gcgtagggac gcaggttgtt
     6961 gtactcgcgc agttgcgcaa cggtgatgcc gtgcgccttc gccaccttcg ccagcgtgtc
     7021 gccgcgcttg tagacatagg acttccacga cgacagcggc ttcttgtgtg cggcgaggtt
     7081 gctgatgaac acgtccgcgc gatcggtcgg cagcacgagc tgcggcgaca cctcgctcga
     7141 caccacggga cggttgttgc ccggattcag cgcgatgagt tcttctaccg gcatttccgc
     7201 cagcttcgcc gccacgttga ggtcgatgtc gcgggtgagg tcgacggtga cgaagtaggg
     7261 cgcgttggga atgggggtga gctcgatgcc gaaggcgtcc gggcgcgcga tgatgttttc
     7321 cagcgcttgc agcttgggca cgtaatagcg cgtttccttc gggatgcgca ggctttcgta
     7381 gtcggtcggt ttgcctttgg cgcggttccg cttcatcgcg cgcagcacgc cgttttcgcc
     7441 catgttgtag gaggcgagcg ccagatgcca gtcgccgtgc aactcgtaca ggaactgcag
     7501 atagtcgagc gcggcgctgg tggaggcgac gatgtcccgg cggccgtcgt accaccagtt
     7561 ctgttccagg ttgtaatact tgcccgtgga ggggatgaat tgccacagac ccgatgcgcg
     7621 tgccgaggaa tgcgccatcg gattgaacgc gctctcgacc atgggcagca gcgccagttc
     7681 ggtgggcatg ccgcgttttt caagctcttc gacgatgtgg tagaggtagc ggcggctgcg
     7741 ctccaccagg acgcgcatct gttccggccg cgccgcatac caggcctggc gctcgaccac
     7801 cagacggccg tccagatccg gcatggcgaa cccgcgacgg atgcgttccc acagatcgtc
     7861 gggcacgtta tccgccagat ctccggcgcg ccgtgcgccc agatcgcgca catcgcgcac
     7921 atccacatcc tcggcgctgt ccgactgcat gcccagggca ggatgccggg cctcaccctc
     7981 ctcgaaaggc aggcggatat cgaagtattc cggcggaaga ctgtgcagga tgggatcgac
     8041 cgcgtgcgcc ggtgcgcagg tcaatgccac ggtcagaatg gcg
//
LOCUS       DATGOU010000116        12590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_1681755, whole genome shotgun sequence.
ACCESSION   DATGOU010000116 DATGOU010000000
VERSION     DATGOU010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 12590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12590
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1681755"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VKZ48_06905"
     CDS             complement(<1..364)
                     /locus_tag="VKZ48_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLU77612.1"
                     /translation="MKILVVDDHPLILEALQHVLQQLDGEVEVLAAQCAQSGRELVAE
                     NADASLLLLDLQLPGAVGMSLLTELRQSFPTVPVVVLSGSDRREDVLQAIDLGAMGFI
                     PKSASNRVMLQALRLILSG"
     gene            675..806
                     /locus_tag="VKZ48_06910"
     CDS             675..806
                     /locus_tag="VKZ48_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77613.1"
                     /translation="MKSKTELNGWAVLADFAGTLGLALAGGIGFSIAAGAVVVMIAG"
     gene            complement(862..1827)
                     /locus_tag="VKZ48_06915"
     CDS             complement(862..1827)
                     /locus_tag="VKZ48_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77614.1"
                     /translation="MRQATPQLAAATAQLRARLADPNQTTRLYGTTPPRLGSPAEVIQ
                     EAAEKLVERLQPLPVDAVVVYDIQDESGRTSVPRPFPFKGTVDPRDYSRLLGQLAGLP
                     AINYKSLGTLDEANWRAWLDQSADDYGVTYLSVVGRPTSGVSYPLSLAEALQMAREHR
                     GSFTLGGVMIAERDSPECSEGARLLAKAGEGCSYFISQTVYNAQPTVAVLKEYLRECR
                     DAGVEPQRIMFTFAPCGREKTMAFIKWLGVRITPECERSILDAPDPLAKSIEICVDNL
                     REILDQDYVGRIPLGINVESVSINRDEIDATVELFHRLSEVLEGK"
     gene            complement(1977..3395)
                     /gene="ahcY"
                     /locus_tag="VKZ48_06920"
     CDS             complement(1977..3395)
                     /gene="ahcY"
                     /locus_tag="VKZ48_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HLU77615.1"
                     /translation="MSAVLQQNQFTDCKVADLSLAEWGRKEIKIAETEMPGLMAIREE
                     YASAKPLRGARITGSLHMTIQTAVLIETLQALGAEVRWASCNIYSTQDHAAAAIAATG
                     TPVYAYKGETLEEYWDFSHKIFEWPDGGYSNMILDDGGDATLLLHLGAKAETDISVLA
                     HPESEEETVLFAAIKAKLAQDPTWYSTRLSKIKGVTEETTTGVKRLYQMHQEGRLAFP
                     AINVNDSVTKSKFDNLYGCRESLVDGIKRATDVMVAGKIAVVCGYGDVGKGSAQALRA
                     LSAQVWVTEIDPICALQAAMEGYRVVTMEEACDKADIFVTATGNYHVITHDHMAKMKD
                     QAIVCNIGHFDNEIDVASLKQYKWENIKPQVDHVIFPDGKRIILLAEGRLVNLGCATG
                     HPSYVMSSSFANQTIAQIELYNNTDKYPVGVYVLPKHLDEKVARLQLKKLNAQLTELT
                     DVQARYIGVAKEGPYKADHYRY"
     regulatory      complement(3414..3547)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            complement(3603..4781)
                     /gene="metK"
                     /locus_tag="VKZ48_06925"
     CDS             complement(3603..4781)
                     /gene="metK"
                     /locus_tag="VKZ48_06925"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HLU77616.1"
                     /translation="MMSEFLFTSESVSEGHPDKVADQISDAILDAILTQDKYARVAAE
                     TLCNTGLIVLAGEITTNAVIDYQTIARETVKRIGYDNTDYGMDYKGCAVLVAYDKQSP
                     DIAQGVDEDKGLNLEQGAGDQGLMFGYACDETPELMPLPIYLAHRMVERQSELRRDGR
                     LPWLRPDAKSQVTVRYAGGKPQEIDTVVLSTQHAPDVTYEQIKEAVVEEIIKPVLPAE
                     MLSKKINYLVNPTGRFVVGGPHGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDR
                     SAAYAARYVAKNIVAAGLAAKCQIQVSYAIGVARPTSVMVTTFGTGKVSDDALAELVQ
                     KHFDLRPKGIVQMLDLLRPIYEKTAAYGHFGRDEPEFSWEKTDKAAALAADAGLKRVA
                     "
     gene            4944..5798
                     /gene="dapF"
                     /locus_tag="VKZ48_06930"
     CDS             4944..5798
                     /gene="dapF"
                     /locus_tag="VKZ48_06930"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008112691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HLU77617.1"
                     /translation="MKLRFTKMHGLGNDFVVLDGVRQTIQLDADRLRAIADRHFGVGC
                     DQILVVEPPRSASADFRYRIFNADGGEVEQCGNGARCFARFVRDKGLTDKRRIAVETL
                     GGLIEPQIEADGRVTVDMGPPAFDPARVPFLADGEALTYDLEVEGETIPVAVLSMGNP
                     HAVQRVADVNEAPVARLGPLVERHARFPQRVNAGFMEVVDRHKIKLRVYERGAGETLS
                     CGTGACAAAVAGIRLDLLDSPVTVHTRGGDLTIAWAGVDNAAARPAHVYMTGPAETVY
                     EGEIIVGD"
     gene            5785..6444
                     /locus_tag="VKZ48_06935"
     CDS             5785..6444
                     /locus_tag="VKZ48_06935"
                     /inference="COORDINATES: protein motif:HMM:NF016243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HLU77618.1"
                     /translation="MSETETLRADDVAQYLEQHPEFFIEHSQLLTRIQVPHPRGTGAI
                     PLSERQVLSLREQNRALENKLAELIQFGEENDDISQRVHRLAVDLMAGSPLSALIECC
                     CRAVRDDFGVPHAAVRLWRGSGESEAFAETSEELRNFAAALPRPFCGPNDNFEAVAWF
                     GEAAALVRSVAFIPLRDAGETIGLLAMGSEDPERFYADMGTLYLERIGELVSAALLRE
                     L"
     gene            6446..7366
                     /locus_tag="VKZ48_06940"
     CDS             6446..7366
                     /locus_tag="VKZ48_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006391908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HLU77619.1"
                     /translation="MNPAAPEADDPRQALVDAYLAHLLQQRRLSPATERGYRQGLRNL
                     LQLAGDKPLDRLETHHVRRMVAQLHGRGLQPSTIAHMLSAWRGLYRWLARHHGYGVDP
                     CAGVRAPKARKGLPKALSPEEARRLLDGVPEDACEARDKAMFELCYSSGLRLAELVSL
                     DLHDGHAMLKQGEITVRGKGNKTRTVPVGRAALQAVEVWLAQRDGVVRGEQEALFLGA
                     QGGRIAPSVVRARLKRWALKQGLPVPVHPHVLRHSFASHVLQSSGDLRAVQEMLGHAS
                     ISTTQVYTHLDFQHLAKIYDQAHPRARKKP"
     gene            7667..8104
                     /gene="dksA"
                     /locus_tag="VKZ48_06945"
     CDS             7667..8104
                     /gene="dksA"
                     /locus_tag="VKZ48_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HLU77620.1"
                     /translation="MKKTSQSSGGERLTEAELLKMSEAEYMNAAQLAFFKERLLAMKQ
                     QLIDNAGATSENLRENDMVPDPADRATIEEEHALELRTRDRERKLMKKIDESLQRIES
                     GDYGWCEETGDPIGIPRLLARPTATLSVEAQQRRELKQKLYGD"
     gene            8343..9764
                     /locus_tag="VKZ48_06950"
     CDS             8343..9764
                     /locus_tag="VKZ48_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HLU77621.1"
                     /translation="MAFSLFGKKPPAKPAKAPVAPRKQEARKPTPVSTPEVEEDALSL
                     DFTNYVPPATVLSEAPAAVAPAPEPVAKAEAPAEAQTSAAPVSLPALDFDSLPGAAPV
                     AAEPAPAEAPAPAAVSVPGGTSAPQTDSGAPLDSILLIEVESGTQEVPTIIEEAAVLY
                     ANGQSQEALDTLNRAIEAGTLESWTLPAWLMRFDLYQQLGRKSEFEEKALHFVVKFER
                     SPPAWNDLPSATTANRPGGSAHIALSGSLSSASKAAFGQMLKASERHPKLHLDFAKLE
                     GADAEGCQLLLDTLATLRKAKKEVYISGEARAFELLRARIAEGGATPLWLLLLDLYQQ
                     LGMQEEFEELAVDYAVTFEVSPPSWEARARVEPPPPPAPVGGGERDDSDDSYRLEGDI
                     AGEQEALFADLQKYVKQANPVLIDLSRTRRIDFINAGRLLHLIEKARESKRAVVLRSV
                     GEMMMALFAVMGIHKHARIIPRK"
     gene            9824..10366
                     /gene="hslV"
                     /locus_tag="VKZ48_06955"
     CDS             9824..10366
                     /gene="hslV"
                     /locus_tag="VKZ48_06955"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020653769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HLU77622.1"
                     /translation="MEQFRGTTIVSVRRAKQVALGGDGQVTLGNIIIKAGARKVRRLH
                     QDKILAGFAGGTADAFTLFERFEAKLDKHQGNLLRSAVELAKDWRTDRILRRLEAMLA
                     VADRESSLIITGNGDVLEPEYGLIAIGSGGAYAQSAARALLENTELDAEAIVRKSLGI
                     AGDLCIYTNQSHTVEVLDWQ"
     gene            10421..11758
                     /gene="hslU"
                     /locus_tag="VKZ48_06960"
     CDS             10421..11758
                     /gene="hslU"
                     /locus_tag="VKZ48_06960"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HLU77623.1"
                     /translation="MSAMTPQEIVHELDKHIIGQHKAKRSVAVALRNRWRRQQVPEPL
                     RQEITPKNILMIGPTGVGKTEIARRLAKLADAPFIKIEATKFTEVGYVGRDVDTIIRD
                     LVEISVKQTREVAMRQVRARAEDAAEERILDALLPPAREMGFGAPSTPSPEESSTRQK
                     FRKKLREGELDDREIEIELAQINPQMEILAPPGMEDLTQQIQGMFQNLGGTRKKLRKL
                     KIKEALKHLVDEEAARLVNDEELKAAAVANAEQNGIVFLDEIDKVASRSETHGADVSR
                     QGVQRDLLPLVEGTTVSTKYGMVRTDHILFIASGAFHLAKPSDLIPELQGRFPIRVEL
                     DSLSVDDFEKILTNTDACLTRQYQALLATEQVHVEFQPSAIKRLAEIAYSVNEKTENI
                     GARRLYTVMERLLEDVSFEAGNRGSQTVVIDAEYVDGRLGDLAQSEDLSRYVL"
     gene            11767..12243
                     /locus_tag="VKZ48_06965"
     CDS             11767..12243
                     /locus_tag="VKZ48_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HLU77624.1"
                     /translation="MSRTLRRALACALIAGFAAPAWAQVTVDNVWVRGTVPSQKVTGG
                     FLTIRSTHGGALVGADSPVSAHVEVHEMRMEGEVARMRRIERLPLPAGEPLELRPGGY
                     HLMLMDLKQPLKAGDKVPLRLEIDAGDGKVQTIEVEAVVRGLGTDAPAHGHGHAAH"
     gene            12329..>12590
                     /locus_tag="VKZ48_06970"
     CDS             12329..>12590
                     /locus_tag="VKZ48_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HLU77625.1"
                     /translation="MANLQRYNDPFGDLFDDLFRGFLVRPVVGEGTQLPQRLKLEVSE
                     EDNQYKVLADIPGVKKEDIHVDINGDQVSIDAEVKQEKEAKEN"
BASE COUNT         2290 a         3936 c         4090 g         2274 t
ORIGIN      
        1 cgcccgaaag gatgagacgg agcgcctgca gcatgacgcg gttgctcgcg cttttgggaa
       61 tgaatcccat ggcgccgagg tcgatggctt gcagtacgtc ttcgcgtcgg tcggagcccg
      121 acagcacgac caccggtacg gtgggaaagg actggcgcag ttcggtcaac agggacattc
      181 cgacggcgcc cggcaactgc aggtcgagca acagcagact ggcgtctgcg ttttcggcaa
      241 ccagttcacg gccactttgg gcgcactgcg ctgccagcac ttcgacctcc ccgtcgagtt
      301 gctgcagcac atgctgtaag gcttcgagga tgagtggatg atcatccacc acgagaatct
      361 tcacagcggc ccttatgggt gacttattgc ttttggctgg gcatggtagc accggccggg
      421 acgcttgcaa acgcgggaaa ttcagcatat ccgagcccgt agggggaaat cccgggcgat
      481 gctaacagcg cgcagccgtc cggcaatgcg gcgaagggca taggcaataa ggcggacatc
      541 agccgcagcc ggcgttccga aacgaaagtg aaacaatgcg caacaccggc gtaattgccc
      601 tttttctgcc tcatttcctc ggtaatctcg cgactctgat acagagccca tgacgggctg
      661 cgcgagacaa caggatgaaa tcaaagacag aactgaacgg ctgggcggtc ctggccgatt
      721 tcgccggcac cctggggctg gcgctggccg gaggcatcgg cttcagcatt gccgccggcg
      781 ccgtggtggt gatgatcgcc ggctgatcgc cgacgaggcc cgaaccgggg gcgagcggct
      841 ttgcaccgcc gccccaccgc gttacttccc ttccagtact tcagagagcc ggtggaacaa
      901 ctccaccgtc gcgtcgattt cgtcgcgatt gattgaaacg ctctcgacat tgatgcccag
      961 cggaatccgg ccgacgtaat cctggtcgag gatttcgcgc aggttgtcga cgcagatttc
     1021 gatggatttg gccagcggat caggtgcatc gagaatgctg cgctcgcatt cgggcgtgat
     1081 gcgcacgcca agccacttga tgaacgccat ggtcttctcg cgcccgcagg gggcgaaggt
     1141 gaacatgatg cgttgcggct cgacgccggc gtcgcggcat tcgcgcaaat actccttcag
     1201 caccgccacc gtcggctggg cgttgtagac cgtctgcgaa atgaagtagc tgcagccttc
     1261 accggctttg gccagcagcc ttgcgccttc actgcattcc gggctgtcgc gctcggcgat
     1321 catcacgccg cccagcgtga agctgccgcg gtgctcgcgg gccatctgca gcgcttcggc
     1381 caggctcagc ggatagctga cgcccgaggt cgggcgaccg acgacggaca gatacgtcac
     1441 gccgtagtcg tcggcgctct ggtcgagcca ggcgcgccag ttcgcctcgt cgagcgtgcc
     1501 caggctcttg taattgatgg ccggcaaccc ggccaactgc ccgagcaacc gcgaatagtc
     1561 gcgcggatcc accgtgccct tgaacggaaa gggacgcggc accgaggtgc ggcccgattc
     1621 gtcctgaatg tcgtaaacca cgaccgcatc cacgggtagc ggttgcagtc gctcgaccaa
     1681 cttctccgcc gcttcctgaa ttacttcggc cggcgagccg aggcgcggcg gcgtcgtgcc
     1741 gtaaaggcgg gtggtctggt tggggtcggc cagccgtgca cgtaactgcg ctgtggcggc
     1801 ggccagttgt ggcgtcgctt gtcgcatgaa cgtgttcctt taagcgtagg ctgccggacg
     1861 cgccggcaac aaactgcatc ccgggacggc acccgtcgtg cgggcgcgcc cggagggtcg
     1921 tcgatgcgga aacgcataca tcgttcccgc accccgctcg tcaaaggttc gcgttctcag
     1981 tagcgatagt ggtcggcttt gtaggggcct tccttcgcca cgccgatgta gcgtgcctgg
     2041 acatcggtga gctcggtcag ctgcgcattg agcttcttca actgcaggcg cgcgaccttt
     2101 tcgtccagat gcttcggcag tacgtacacg ccgacagggt acttgtcggt gttgttatag
     2161 agctcgatct gcgcgatggt ctggttcgca aacgaagagc tcatcacata agaggggtgg
     2221 ccggtcgcgc agccgaggtt caccaggcgg ccttcggcca gcaggatgat gcgcttgccg
     2281 tcggggaaga tgacatggtc gacctgcggc ttgatgttct cccacttgta ctgcttgaga
     2341 ctcgcgacgt cgatctcgtt gtcgaagtga ccgatgttgc agacgatggc ctggtccttc
     2401 atcttggcca tgtgatcgtg cgtgatcaca tggtagttgc cggtagcggt aacgaagatg
     2461 tctgccttgt cgcaggcttc ttccatggtc acgacccgat aaccttccat cgccgcctgc
     2521 agcgcgcaga tgggatcgat ttccgtcacc cacacttgcg cggacagcgc gcgcagcgcc
     2581 tgcgccgaac ccttgcccac gtcgccgtag ccgcagacga cggcgatctt gccggcgacc
     2641 atcacgtcgg tcgcgcgctt gatgccgtcc accagcgact cgcggcagcc gtacaggttg
     2701 tcgaacttcg acttggtgac cgagtcgttg acgttgatgg cagggaaggc aagacggcct
     2761 tcctggtgca tctgataaag gcgcttcacg ccggtggtgg tttcctcggt gacgcccttg
     2821 atcttcgaca ggcgcgtgga ataccacgtc ggatcctgtg caagcttcgc cttgatggcg
     2881 gcgaacagca ccgtctcttc ctcgctttcc gggtgcgcga gcaccgagat gtcggtttcg
     2941 gctttcgcgc ccaggtgcag cagcagggtg gcatcgccgc cgtcatcgag gatcatgttc
     3001 gagtagccgc cgtcgggcca ctcgaaaatc ttgtgcgaga aatcccagta ctcttccagc
     3061 gtctcgccct tgtaggcgta gaccggcgtg ccggtcgcgg cgatggccgc cgctgcatga
     3121 tcctgggtcg agtagatatt gcaggacgcc cagcgcactt ccgcgcccag cgcctgcagg
     3181 gtttcgatca gtacggcggt ctggatggtc atgtgcagac tgccggtgat gcgcgcgccc
     3241 cgtagcggct tggccgaggc atactcctcg cggatggcca tgagaccggg catttcggtc
     3301 tcggcgattt tgatttcctt gcggccccac tcggcgaggc tgaggtcggc gactttgcag
     3361 tcggtgaatt ggttttgctg cagtactgcg ctcatggcgg ctccgttcga tggtttgaac
     3421 gggcgccgtt gcaacagtgt cgtcgttgtg ccgagcctgg cctgcccacc aaagcggttc
     3481 gacagccatc tttcggcgat gacgttcgag ccgctttggt gggcaggctg cagcgcccct
     3541 cggcgcaacg aaggtgggat gttaagcgat agtcgaaacc tttttaagtc ccgggtcgtg
     3601 cactaggcga cgcgcttcag acctgcgtcg gcggccagcg ccgcggcctt gtccgtcttc
     3661 tcccagctga attccggctc gtcacgaccg aagtggccgt aggcggcggt cttttcgtag
     3721 atcggacgca gcaggtcgag catctgcacg atgcccttgg gccgcaggtc gaagtgcttc
     3781 tgcaccagtt cggccagtgc atcgtcggac accttgccgg tgccgaaagt ggtgaccatg
     3841 acactggtgg ggcgcgccac gccgatggcg taggacacct gaatctggca tttggctgcc
     3901 agaccggcgg ccacgatatt cttcgccacg tagcgcgcgg cataggcggc cgagcggtcg
     3961 actttggacg ggtccttgcc cgagaacgcg ccgccgccgt gcggcgcggc gccaccataa
     4021 gtgtcgacaa tgatcttgcg tccggtgaga ccgcaatcgc cgtgcgggcc gccgacgacg
     4081 aagcgaccgg tggggttcac caggtagttg atcttcttgc tgagcatctc ggccggcagc
     4141 accggcttga tgatttcctc gacgaccgct tccttgatct gctcgtacgt cacgtccggg
     4201 gcgtgctggg tggatagcac aaccgtgtcg atttcctgcg gcttgccgcc cgcataacgg
     4261 acagtgacct gcgattttgc gtccggacgc agccacggca aacggccgtc gcgacgcagt
     4321 tcggactgac gctccaccat gcggtgcgcc agatagatcg gcagcggcat cagttcgggc
     4381 gtctcgtcgc aggcgtagcc gaacatcagg ccctggtcgc cggcaccctg ttccagattg
     4441 aggcccttgt cctcgtccac gccctgcgcg atatcgggcg actgtttgtc gtaggccacc
     4501 agcacggcgc agcccttgta gtccatgccg taatcggtgt tgtcgtaacc gatgcgcttc
     4561 acggtctcgc gcgcgatggt ctgatagtcg atcaccgcgt tggtggtgat ctcgccggcg
     4621 agcacgatca ggccggtgtt gcagagcgtt tccgcggcga cgcgcgcata cttgtcctgc
     4681 gtcagaatcg catcgagaat ggcgtcggaa atctggtccg cgactttatc gggatggcct
     4741 tccgaaaccg attcggaagt gaacaggaat tcgctcatga tggtgggttc gttggatcag
     4801 ggtcgggagt ttagcaaatt ggatggggca acacagggcg ctccgcgggc ggcgggaagc
     4861 ttctaggcaa gtcacatgcc ctgtccagcg cgcggccatc ccgtgcacta tatacataac
     4921 tcgccatacc acggcagtgc cctatgaaac tgcggttcac caaaatgcat ggcctgggca
     4981 acgacttcgt cgtgctcgac ggtgtgcgcc agaccatcca actcgacgcc gatcggctgc
     5041 gcgccattgc cgatcgccat ttcggcgtcg gctgcgacca gattctagtg gtagagccgc
     5101 cccggtcggc atcggccgat tttcgctatc gcatcttcaa cgccgacggc ggcgaagtgg
     5161 agcaatgcgg caacggcgcg cgctgtttcg cgcgcttcgt gcgggacaaa gggctgaccg
     5221 acaagcgccg catcgccgtg gagacgctgg gcggcctcat cgagccgcag atcgaagcgg
     5281 acggtcgggt gaccgtcgac atgggaccgc ccgcattcga cccggcgcgc gtgccttttc
     5341 ttgccgacgg cgaggcgctg acttacgacc tggaagtcga gggcgagacc attccggtgg
     5401 cggtgctttc catgggtaat ccgcatgcgg tgcagcgggt ggccgatgtg aatgaagcgc
     5461 ccgtcgcccg gctcggcccg ctggtcgagc gccatgcgcg ctttccgcag cgcgtgaacg
     5521 ccggcttcat ggaagtcgtc gaccgccaca agatcaagct gcgggtttac gagcggggcg
     5581 cgggcgaaac gctgtcctgc ggtaccggag cctgcgccgc tgccgtcgcg ggcattcgac
     5641 tcgatctgct ggattcaccc gtgacagtgc acactcgcgg cggagatttg acgatcgcct
     5701 gggcgggcgt cgacaatgcc gccgcccggc cggcgcacgt ctatatgacc ggcccggccg
     5761 agacggtata cgagggagag atcattgtcg gagactgaaa cgctgcgcgc ggacgacgtt
     5821 gcccagtacc tggagcagca ccccgaattc tttatcgaac attcgcaact gctgacgcgt
     5881 attcaggtgc cgcatccccg cggcacgggc gcgatcccgt tgtctgaacg ccaggttctg
     5941 tcgctgcgcg agcagaaccg ggcgctggaa aacaagctcg ccgagttgat ccagttcggc
     6001 gaggaaaacg acgacatcag ccagcgcgtc caccgtttgg cggtcgatct gatggccggg
     6061 agcccattgt ccgcactgat cgagtgttgc tgccgcgcgg tgcgcgacga tttcggcgtg
     6121 ccgcacgccg ccgtccgctt gtggcgcggc agcggcgaga gcgaggcttt cgccgagacg
     6181 agcgaggaac tgcgcaactt tgcggctgcg ctgccccggc ccttttgcgg tccgaacgac
     6241 aacttcgaag ccgtcgcctg gtttggcgaa gcggctgcgc tggtgcgttc ggtcgccttc
     6301 attcccctgc gcgatgcggg tgaaacgatc ggcctgctgg ccatgggcag cgaggatccc
     6361 gaacgcttct atgccgacat gggtacgctc tatctcgagc gcatcggcga gctcgtcagc
     6421 gccgcgctcc tgcgcgagct ttgacatgaa cccggccgcg ccggaggcgg acgatccgcg
     6481 ccaagcgctg gtcgatgcct atctggcgca cctgctccag cagcggcgct tgagccctgc
     6541 gacggagcgc ggctatcggc agggtttgcg gaacttgttg caactggccg gcgataagcc
     6601 gttggaccgg ctcgagacgc atcatgtgcg tcggatggtg gcgcaactgc acgggcgcgg
     6661 gttgcagccg agcaccattg cccacatgct ctccgcctgg cgtggacttt atcgctggct
     6721 tgcgcgccac cacggctacg gcgtggatcc gtgcgccggc gtgcgcgcac cgaaggcgcg
     6781 caagggtttg cccaaagcgc tgtcgcccga ggaggcgcgc cgcctgctgg acggcgtgcc
     6841 cgaggatgcg tgcgaggcgc gcgacaaggc gatgttcgaa ctgtgctatt cctccggact
     6901 gcgtctggcg gaactggtat cgctcgattt gcacgacggg cacgccatgc tcaagcaggg
     6961 cgagataacg gtgcgcggca aggggaacaa gacacgcacc gtgccggtgg gccgcgccgc
     7021 tctgcaagct gtggaggtgt ggctggcgca gcgcgacggc gtcgtgcgcg gtgagcagga
     7081 ggcgctgttc ctcggcgccc agggcgggcg catcgcgccc agcgtggtgc gtgcacgcct
     7141 caaacgctgg gcgctgaagc agggcctgcc tgtcccggtc catccgcacg tgctacggca
     7201 ctccttcgcc tcccacgttc tgcagtcctc gggcgatttg cgcgcggtgc aggagatgct
     7261 cggtcacgcc agcatttcca ccacgcaggt ctatacccac ctggattttc agcatctggc
     7321 gaaaatctac gatcaggcgc atccgcgcgc ccgcaagaag ccgtagcgcg atccatcccg
     7381 gtgcgtcatg ccgggtttcg ccgccccatc ccgtctcatt ccatagaggt tgcctcgcct
     7441 gggggtgcag cctacaatgc gccgccgttt tgagcggctg ggcggataac aggcacgatc
     7501 cgcgctatat gcgtcccgta ttgcccgagg cgtgacctgg aggacggcac gggaacaccg
     7561 atggggtagt atcccaagcg gcgatgggcg gcacgatttg atcgtgcgca aatcgctgca
     7621 ggacgtatac attcgaggac tgaaggcaat ggctcgtcaa accgcaatga aaaaaacttc
     7681 gcaatcctcc ggcggtgaac ggctcactga agcggaactg ctgaaaatgt ctgaagccga
     7741 gtacatgaac gccgcgcaac tggcgttctt caaggaacgc ctgctggcca tgaagcagca
     7801 gcttatcgac aatgccggcg cgacttcgga gaacttgcgc gagaacgaca tggtccctga
     7861 tccggccgac cgcgcgacca tcgaagagga gcatgcgctg gaactgcgca cgcgtgatcg
     7921 cgaacgcaag ctgatgaaga agatcgacga atcgctgcaa cgcatcgagt cgggcgacta
     7981 tggctggtgc gaggagaccg gcgatccgat cggcatcccc cgtctgctcg cgcggccgac
     8041 cgcgacattg tcggtcgagg cacagcagcg ccgcgaactc aagcagaagt tatacggcga
     8101 ttgaccgccc tggatgcata gcgaagaacg gtcgccttgg gcggccgttc ttgttttggg
     8161 gcgctgccgt tcatccccgt cgtgcggccg ctgggcggat cgttgcgcgc gtggcctgaa
     8221 gtttgctcgg agcggcaata tcggacgctg tcgcgcactt gccatttggt attgcgtcag
     8281 acggtatggt agaagaatgc tttgagccga ccttccgttc tccactaaat caagtcttta
     8341 acgtggcttt ttcgcttttc ggtaaaaaac cgccggccaa gcctgcgaag gcccctgttg
     8401 caccgcgcaa acaggaggcg cgcaagccaa cacccgtatc gacgccggaa gtcgaggaag
     8461 acgcgttgtc cctggatttc accaactatg tgccgccggc gacggtattg agcgaggcac
     8521 ccgccgcggt tgcgccggcg cctgagccgg tggccaaggc ggaggcgcct gccgaagcgc
     8581 aaacgtccgc ggcgccggtt tcgctgccgg cgctcgattt cgattccctg ccgggtgccg
     8641 cacccgtcgc cgccgaaccc gcgccggccg aagcgcctgc cccggccgcc gtgtccgtgc
     8701 cgggcggaac gtccgcgccg cagaccgaca gcggtgcgcc gctcgactcg atcctgctga
     8761 tcgaagtgga gagcggcacg caggaagtgc cgacgatcat cgaggaagcg gcggttttgt
     8821 acgccaatgg gcaatcgcag gaggcgctcg acacgctcaa ccgcgccatc gaagccggca
     8881 cgctggagag ttggaccctg ccggcctggc tgatgcgctt cgacctctat cagcagttgg
     8941 gccgcaagag tgagtttgag gaaaaagcgc tgcacttcgt cgtcaagttc gagcgctcgc
     9001 cgccggcatg gaacgacctg ccgagcgcga ccacggcgaa ccgcccgggc ggttcggcgc
     9061 atatcgcgct gtccggttcg ctcagttcgg cgagcaaggc ggcgttcgga cagatgctca
     9121 aggcttccga gcgccatccc aagctgcatc tggatttcgc caagctggag ggcgcggatg
     9181 cggagggctg ccagcttttg ctggatacgc tggccacgct gcgcaaggcc aagaaagagg
     9241 tttatatcag cggcgaagcg cgcgccttcg agttgctgcg cgccagaatc gcagagggcg
     9301 gcgccacgcc gctatggctg ctgctgctcg acctctatca gcaattgggc atgcaggaag
     9361 aattcgaaga gctggccgtg gattacgccg tgaccttcga agtctcgccg ccctcctggg
     9421 aggcgcgtgc acgggtcgag ccgcccccgc cgccagcgcc ggtgggtggc ggtgaacggg
     9481 acgacagcga cgacagctac cggctcgagg gcgatatcgc cggcgagcag gaggcgctgt
     9541 tcgcggatct gcagaaatac gtcaagcagg ccaatcccgt cctcatcgac ctgtcgcgca
     9601 cgcgccgcat cgacttcatc aacgccgggc ggctgctgca tctcatcgaa aaggcgcgcg
     9661 agtccaagcg cgcggtggtg ctgcgctcgg tcggcgagat gatgatggcg ctgttcgccg
     9721 tcatgggcat tcacaagcac gcccgcatca tcccgcgcaa gtaaggttga aatttcgcgg
     9781 aattgccgca atatcggatg aaccatttct tccgggttct tccatggagc aattccgcgg
     9841 taccaccatc gtgtccgtcc gccgtgccaa gcaggtcgcc ctcgggggcg acggtcaggt
     9901 gacgctgggc aatatcatca tcaaggcggg cgcgcgtaaa gtgcgccggt tgcaccagga
     9961 taagatcctc gccggcttcg ccggcggcac cgccgacgcc ttcacgctgt tcgagcgctt
    10021 cgaggccaaa ctcgacaagc accagggcaa tctgctgcgt tcggccgtgg aactggctaa
    10081 ggactggcgc accgatcgca ttctgcgccg cctggaagcc atgctcgccg tggccgatcg
    10141 cgaaagctcg ctgatcatta ccggtaatgg cgacgtgctc gaacccgaat acggcctgat
    10201 cgccatcggc tccggcggcg cgtatgcgca gtccgccgcg cgcgccttgt tggaaaacac
    10261 cgaactggat gccgaagcca tcgtgcgcaa gagtcttggc atcgccggcg atctttgcat
    10321 ttacaccaac cagagccaca cggtcgaagt cctcgactgg cagtgagcgt agcgcgcgcc
    10381 cggcaaggcg ccgttttcag ctatcgataa aggcatgacc atgtccgcca tgacgccgca
    10441 ggaaatcgtc cacgagctgg acaaacacat catcggtcag cacaaagcca agcgctccgt
    10501 agcggtggcg ctgcgcaacc gctggcgccg ccagcaggtg cccgagcccc tgcgccagga
    10561 gatcacgccc aagaacattc tcatgatcgg gccgaccggc gtgggcaaga ccgaaatcgc
    10621 gcggcggctg gcgaagctcg ccgacgcgcc ctttatcaag atcgaagcga ccaaattcac
    10681 cgaagtcggc tatgtcggcc gcgacgtgga caccatcatt cgcgatctgg tggagatcag
    10741 cgtcaagcag acgcgcgagg tggcgatgcg tcaggtgcgc gcccgcgccg aagatgctgc
    10801 cgaagagcgc atcctcgatg cgctgctgcc gccggcgcgg gaaatgggct tcggcgcgcc
    10861 gagcacgccc agcccggagg aatcgtctac gcgacagaaa ttccgcaaga agctgcgcga
    10921 aggcgaactg gacgatcgag aaatcgaaat cgagctggcg cagatcaatc cgcaaatgga
    10981 aatcctcgcg ccgcccggca tggaagacct gacccagcag attcagggca tgttccagaa
    11041 cctcggcggg acgcgcaaga agctgcgcaa gctgaagatc aaggaagcgc tcaagcacct
    11101 cgtcgacgag gaagcggcgc gactggtcaa cgacgaggaa ctgaaagccg ccgccgtggc
    11161 caatgccgag cagaacggca tcgtgttcct cgacgagatc gacaaggtgg cgagccgcag
    11221 cgaaacgcac ggtgcggacg tgtcgcgcca gggcgtgcag cgcgacctgt tgcccctggt
    11281 ggaaggcacc accgtctcca ccaagtacgg catggtgcgt accgaccaca tcctgttcat
    11341 cgccagcggc gccttccacc tggccaaacc ttccgatctc attccggagc tgcagggccg
    11401 tttccccatc cgggtcgaac tggactccct gtccgtggac gacttcgaga agatcctcac
    11461 caatacggac gcctgcctca cgcggcagta tcaggcactg ctcgcgacgg aacaggttca
    11521 tgtcgaattc cagcccagcg cgatcaagcg gctcgccgaa attgcctact ccgtcaacga
    11581 gaagaccgag aacatcggcg cccgccggct ctataccgtc atggagcgac tgctcgagga
    11641 cgtgtccttc gaagccggca atcgcggatc gcagaccgtg gtcatcgatg ccgaatatgt
    11701 ggacggcaga ctcggcgacc tcgcgcaaag cgaggatctg tcgcgctacg tgctatgagg
    11761 accggcatga gcagaacgct gcgccgcgcg ctggcatgcg ccttgatcgc cggcttcgcc
    11821 gcgcccgcct gggcgcaggt gacggtggac aacgtctggg tgcgcggtac ggtgccgagc
    11881 cagaaagtca ctggcggctt cctcaccata cggagcaccc atggcggcgc tctcgtgggc
    11941 gccgattcgc ccgtctccgc gcatgtggag gtgcacgaaa tgcgcatgga gggcgaggtc
    12001 gcgcgtatgc gccggatcga acgtctgccg ttgcctgcgg gcgagccgct cgaactgcgg
    12061 ccgggcggtt atcacctgat gctcatggat ctcaagcagc cgctcaaggc cggcgacaag
    12121 gtgccgttgc ggctcgagat cgacgcgggc gacggcaagg tgcagaccat cgaagtcgag
    12181 gccgtcgtac gcggtctggg caccgacgcc ccggcgcacg gacacggaca cgctgcccac
    12241 tgagcggcgc ttgaaactac ggtcacgctc cccatttatt gagtcatcgg gaacgccccc
    12301 gacgtatgac tgaacaggag agacgaccat ggcgaatctg caacgttaca acgatccctt
    12361 cggggacctt tttgacgatc tgttccgcgg cttcctggtg cggccggtgg tgggcgaagg
    12421 tacgcaactg ccccagcggc tcaagctgga agtcagcgaa gaggacaacc agtacaaagt
    12481 gctggccgac attcccggcg tgaaaaagga ggacatccat gtcgacatca acggcgacca
    12541 ggtgtcgatc gacgccgaag tgaaacagga gaaggaagcc aaggaaaacg
//
LOCUS       DATGOU010000117         9690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_1682013, whole genome shotgun sequence.
ACCESSION   DATGOU010000117 DATGOU010000000
VERSION     DATGOU010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9690
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682013"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..6369
                     /locus_tag="VKZ48_06975"
     CDS             103..6369
                     /locus_tag="VKZ48_06975"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HLU77626.1"
                     /translation="MNHIYRVVWNESLGVWQAVAEHARSRGKTKSKRLRSLALAVALA
                     FGGGALAADLPTGGTITAGSGSIHQNGAHLTVNQGSGKLAIDWQSFSIGKHNSVTFNQ
                     PSASAVALNRVLGPDVSVIQGALNANGQVFLINPNGVLFTPTAQVDVGGLVASTLNMS
                     TEDFLAGNYAFEGDSAGSIVNQGSITAAQGGTVALIAARVENSGSITAHGGNVLLGSG
                     SKVTLDLGGPVKLEVEEGAIEALIKNGGAIKADGGTILLTAKAAEDLASTVINNDGII
                     EAQTLTTGERGEIVLLGENGTVEVAGTVDVSAPEGQGGKAVVTGERVLIADGARINAT
                     GAQGGGEIYVGGGWQGEDPAIKQATATVVMQGATLDASATDNGNGGTVVAWSDVRKAD
                     SITRVAGELKARGGANSGDGGSIETSGHSLEVGSAPDVSAAQGQGGKWLIDPYNITVV
                     EGSVRTNINNDSPFASTGDSAELGIDLIDAALKNGEVVIQTAGPTGDGEQGGDITWNV
                     EYAYTGGVARTLTLNAHRDIILNESIRSVDGALSMNFNADFDDSGAGGTVVRKDLESM
                     GGAIRFGGLGTIFSGESAQKINTSSGASGGALHFYGEVLLANPEGLTITTSGGNVDFD
                     RVLDAGNRYTYDGTLRSWVDAKAAVESGAGDAVGSTYLATITSALENTAAMAAAAGRE
                     AWLGGSDAGVEGTWRWVTGPEGMEDGGQGRVFHNGSTQGLSGYVGANGSFVNWNRGEP
                     NNSGDEDALQLGAGAQGQWNDLSTTSASLGSIVETNLAASPLIIDAGAGRVTFGDRVG
                     AGKALASLNVTASEIVINGDSVRTEGAQTYNGNVEFGSLQQFVNPSFEDGLKGWTIVN
                     DRIYFSGHNGSTIGGFETPVDTTYPDWVVSGCSTATGVNCDTTPLSNTWSFTTKTSSD
                     VAPGSGTQSLVMNSSGSCTEGFCIVRGPYVISDSTVTLPEGGMVSFKWQAKGGGDAYD
                     VYGYLLNTATGKVQTILDDTGASQYTQQPWTTASVSVDEPGTYKFVFVSGTWDATGGR
                     VAGAQLYIDDIKAAGGAKAIDAAQVTFNGTVEAADNQLTVQADKIDFHDAFSGTGRLT
                     LQQRTDSHAIEIGGAANHAADTLDITGAELALLQDGFKSITIGSSTGTGSTTVVGASQ
                     FTDDVTLRSGTGGIALHDSVTATAGNTLTLDTTGTATQTSAGSITADGLALRGAGGTH
                     RLDTAANEVDMLAADTGSVTFVSTGALTIGSVGKTEGINATGKIHVDTPTGDLTIARD
                     VNTSDASDDAIVLNAGRDAGVGDTTGGNVLVSGGVTVEAGAGGRAILYTGGVAGSTGV
                     TNLVGSGSGRFRYNSDESSTNYDKALESGLYAIYREAPTVTIRTNNHSKVYDGQAYTG
                     AGGITTIGLHNGDQLNAGTYSGGGIDVGTYTLSYSTTDSGVGYAVTYDNSGTLTVTPR
                     PVNVSANAVTKTYGDADPALSYAVETASGGRGLLAGDTLDGALSRAAGEDAGAYAINV
                     GTLANGNYSINYAGANFTIAKRAITLAADAVSKIYGEADPTLSVRITGGSLGSETVSD
                     ALSDVVGMLSRAAGENVGAYDVALGTGAKAANYDISFDTDNNALTITPRPVDVTAIAA
                     NKTYGDADPALSYVVETQSVGRGLLAGDALGGALDRVAGEDVGTYAIGQGTLADGNYS
                     INFTGAELTIAKRAITLAADAVSKIYGEADPTFSISIVGGSLGSETVSDALADVVGAL
                     SRAAGENVGAYDVALGTGAKAGNYDITFDADNDALTITPRPVDVIANAATKTYGEADV
                     LTYTTEAQTAGRGLLAGDTLSGSLDRAAGEDVGTYAIGHGTLENVNYSINFTGANLTI
                     TPRPVTVTANNRQKMQGTSDPALTYVAEGQSAGRGLVHGDSLAGLLSRASGEGLGDYA
                     IGAGSLTDANNPNYVITFKDGVFRIVAATEVETSQHVAQVLPENITQGHDTVPQGDPA
                     SFGDQGSGPQTQGGGLRFVDAPADEPSNVGSGSDAGGTPGAGDSGLANAGETTGGASD
                     PGPQSGSIASSDGGVNNADGSASAASTAESGESGNEDAGPDGQFDVAERSDLGPLTVL
                     VVKGGIKLPSFDRQ"
     gene            6477..8117
                     /locus_tag="VKZ48_06980"
     CDS             6477..8117
                     /locus_tag="VKZ48_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HLU77627.1"
                     /translation="MRLSVLALLLMASPCILAQTPPDAGRLLQDMEIPPQPIPPSIDL
                     KLEERAPTDDAAPGGPQVELRTVRFNGMTRFDEAQLQQVIADAIGQSFDLAGLRNLAH
                     RVTRFYREQGYPFARAYLPPQRMSEGELRIEILEGRYGKVTAVGEPELAAGAQAFLSS
                     LQSGEVIEGKSLERAALILGDQPGVKLTPVVRPGEEVGTGDLEARVSRGPAFEARFSA
                     DNHGNRYSGRHRAIAAANWNSPFMLGDQLSASAMLTDESQWFGQLGYALPLGASGLRG
                     QISYAHTYYELGKEFSNLDATGTAKVASVGLSYPLVRTRAHNLLLSVQYQHKDLVDEY
                     GTVDVKNEKSSDSVPVSLQFDARDQLAGGGITYGALAWTVGDLSLDSRDLRNQDRLTA
                     KTEGGFQKWNFDIARLQRLPQNFTLFGRFSGQLTGDNLDSSESFSLGGASGVRAFPQG
                     EGAGDRGWLTQVELRYTMGEFAPYVFYDAGKVTINAKTWDDSDNHRSISGFGAGVRWT
                     CGNIGVDASVAWVAHGGDPESDKHDTQPRFWIKAEYRL"
     gene            complement(8149..>9690)
                     /locus_tag="VKZ48_06985"
     CDS             complement(8149..>9690)
                     /locus_tag="VKZ48_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011870155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HLU77628.1"
                     /translation="HRPLFARNVVATSQPLAAQAGLAVLAKGGNAVDAAIAAAVTLTV
                     VEPISNGVGSDAFALVWDGERLHGLNASGRAPAAWTPDYFQQRIPLRGWNSVTVPGAV
                     AAWVDLSERFGVLPFEELFEAGIHYAREGFHVTPFIAHRWALQIPDLHNQPGFAEAFM
                     PGGRAPHEGELFRLPALARTLAHIAETQGEAFYRGVYAERLAAHAAAHRGAMTEADLA
                     AHTNDWVEPLAVNYRGYTLHELPPNGQGIVALIALGILRHFDLSAYPVDGPDSVHLQI
                     EAVKLAFADVYRYVADPQAMPFPPDALLDPDYLAQRARLIRPDVAQDYDHGEPPRGGT
                     VYLTAADEHGMMVSFIQSNYMGFGSGVVVPGTGISLQNRGAGFSTRADRPNCVAPGKR
                     PFQTIIPGFVTKDGEALMSLGLMGGTMQPQGHVQLMVRMADYNQSPQSAIDAPRFRVE
                     QGLKVNFEAFPPETVAELARRGHQPTELPPAYHDFGCAQIIRRIEGGYVGCCDMRRDS
                     LAVGF"
BASE COUNT         1872 a         3030 c         3279 g         1509 t
ORIGIN      
        1 aagcgaacaa cttggtgatt gcgcgagccg tccgggaacg gctcgcgggg tctcgtgcgg
       61 acaaaccagc cctatggctg atgcgtaaag agctataaaa ccatgaatca catctatcgc
      121 gtcgtatgga atgaatcgct cggcgtctgg caggcggtgg cggagcatgc gcgaagccgc
      181 ggcaagacca aatcaaagcg gctgcgcagc ctggcgcttg cggtggcctt ggcgttcggt
      241 ggtggcgcgc ttgcggcgga tctgccgacc ggcggaacga ttacggcggg cagcggttcg
      301 attcatcaga acggcgcgca tctgaccgtc aatcagggca gcggcaagct ggcgatcgac
      361 tggcagagct tctccatcgg caagcacaac agcgtcacct tcaatcagcc ttccgccagt
      421 gccgtggcgc tgaaccgggt gctcggcccc gacgtctcgg tgatccaggg cgcgttgaac
      481 gcgaacgggc aggtgtttct gatcaacccc aacggtgtgc tgttcacgcc gacggcgcaa
      541 gtggacgtcg gcggcctggt cgcctcgaca ttgaatatga gtacggagga ttttctcgcg
      601 ggcaactacg ccttcgaggg cgatagcgcc ggctcgatcg tcaaccaggg cagcatcacc
      661 gccgcgcagg gcgggactgt cgcgctgatc gcggcgaggg tggagaacag cggcagcatc
      721 acggcgcacg gcggcaacgt gctgctgggt tcgggcagca aggtcacgct cgatctgggt
      781 gggccggtga aattggaagt ggaagaaggt gcgatcgaag cgctgattaa aaacggcggc
      841 gcgatcaagg cggacggcgg cacgatcctg ctgactgcaa aggcggcgga agatctggcg
      901 agcacggtga tcaacaacga cggcattatc gaagcgcaga cgctgaccac aggcgagcgc
      961 ggcgagatcg tattgctggg tgagaacggt acggtggaag tggcgggcac ggtcgacgtg
     1021 tcggcgccgg aaggccaggg cggcaaggcg gtcgtgacgg gcgagcgtgt gctgatcgcg
     1081 gacggcgcac gtataaacgc cacgggcgcg caaggcggcg gggagattta tgtaggcggt
     1141 ggttggcagg gcgaggaccc ggcgatcaag caggcgacgg cgacggtcgt catgcaaggc
     1201 gcaaccctcg acgcttccgc cacggacaac ggcaatggcg gcacggtggt ggcgtggtcg
     1261 gatgtgcgca aggcagactc gatcacgcgt gttgcgggtg agttgaaggc gcgcggcggc
     1321 gcgaatagcg gcgacggggg aagcatcgaa accagcggcc acagtttgga agtcggcagc
     1381 gcgccggatg tgagcgccgc gcaagggcag ggcggaaaat ggctgattga tccctacaac
     1441 atcacggtgg tcgaaggcag tgttcgcacc aacatcaata acgacagtcc gtttgcaagc
     1501 accggtgaca gtgccgaact cggcatcgac ctcattgatg cagcgctgaa gaacggcgaa
     1561 gtcgtcatcc aaaccgccgg ccccacgggt gacggcgaac agggtggcga tatcacctgg
     1621 aacgtcgagt acgcctacac gggcggcgtc gcgcgcacct taaccctcaa cgcccatcgc
     1681 gacatcatcc tgaacgaatc gatccgcagt gtcgacggcg cgctgtcgat gaacttcaac
     1741 gccgatttcg atgacagcgg tgcgggcggc acagtcgtgc gcaaggacct ggagagcatg
     1801 ggcggcgcga tccgcttcgg cggactgggt acgatcttca gcggcgagag cgcgcagaag
     1861 atcaatacgt ccagtggcgc atccggcggc gcactgcatt tctacggtga agtactcctg
     1921 gccaatcccg agggactgac gatcaccacc agcggcggca acgtcgattt cgaccgtgtg
     1981 ctcgatgccg gtaatcgcta tacgtacgac ggcaccttga gaagctgggt cgacgcgaag
     2041 gcagctgttg aaagcggcgc cggcgacgcc gtgggcagca cctacctggc gaccattacc
     2101 tccgcactgg agaacactgc cgccatggcg gccgccgcgg gtcgtgaggc ctggctgggc
     2161 ggctccgatg ccggggtcga gggcacgtgg cgctgggtca ccgggcccga aggcatggag
     2221 gacggcggcc agggccgggt gttccataat ggttcgactc aaggcctgag cggctatgtc
     2281 ggcgccaacg gatccttcgt caactggaac cgcggcgaac cgaacaactc gggcgacgag
     2341 gacgcgctgc aactcggcgc gggagcgcaa ggccagtgga atgatctttc gacgacaagc
     2401 gcgtctctcg ggtcgatcgt cgagacgaat cttgccgcct cgccgctcat catcgacgcg
     2461 ggcgcagggc gtgtcacctt cggcgatcgc gtcggcgcgg gcaaggcgct cgcttcgctg
     2521 aacgtgacgg ccagcgagat cgtcatcaat ggcgacagcg tccgcaccga gggagcgcag
     2581 acctacaacg gcaacgtcga attcggcagc ctgcagcagt tcgtcaaccc gagcttcgag
     2641 gacgggctga agggttggac gatcgtgaac gatcgcatct atttcagcgg ccacaacggt
     2701 tcgacgatcg gcgggttcga gacaccggtc gataccacct accccgactg ggtagtcagc
     2761 ggttgctcga cggcgaccgg tgtcaactgc gacaccaccc cgctaagcaa cacgtggtca
     2821 ttcaccacga aaacgagcag cgatgttgcc ccgggcagcg gcacacaatc gctggtcatg
     2881 aattcctcgg gttcgtgcac cgagggtttc tgcatcgtgc gcgggccgta cgtcatcagc
     2941 gacagcacgg ttacgcttcc cgagggcggg atggtttcct tcaaatggca ggccaagggc
     3001 ggcggcgacg cctatgacgt gtacggctat ctgttgaaca cggccaccgg aaaggtgcag
     3061 accattctcg acgataccgg cgccagccaa tacacgcagc aaccctggac gacggcgtcc
     3121 gtcagcgtgg atgagccggg tacctacaaa ttcgtcttcg tctccggcac ctgggacgcg
     3181 accggcggca gagtggccgg cgcgcagctg tatatcgacg acatcaaggc ggcgggcggc
     3241 gcgaaagcca tcgacgccgc ccaagtgacg ttcaacggaa cggtggaggc ggccgacaac
     3301 caactcaccg tgcaggccga caaaatcgac ttccatgatg cattcagcgg cacgggccgg
     3361 ctgacgctgc agcagcgcac cgactcgcat gcgatcgaaa tcggcggcgc cgcaaatcat
     3421 gcggccgaca cgctggacat caccggggcg gagcttgccc tgctgcagga cggcttcaag
     3481 tccataacca tcggttcgtc caccggcacg ggcagcacca cggtcgtcgg cgcttcccag
     3541 ttcacggacg atgtgacgct gcgcagcggc acaggtggta tcgcgctgca tgacagcgtt
     3601 accgcaacgg ccggcaacac gctcaccctg gatacgacgg gtacggccac gcagacgagc
     3661 gcgggcagca tcactgcgga cgggttggcg ctacgcggcg cgggcggtac gcaccggctc
     3721 gatacagcgg cgaacgaggt cgacatgctc gcggccgaca ccggttcggt gaccttcgtc
     3781 agcacgggcg cactcaccat cggcagcgtc ggcaaaacgg aaggcatcaa cgcaacgggc
     3841 aagatccatg tggacacgcc caccggcgac ttgacgatcg cccgggacgt gaacacctcc
     3901 gacgcctcgg acgacgccat cgttctcaac gcgggccgcg atgcgggcgt cggcgatacc
     3961 accggcggca acgtgctggt ctcgggcggc gtcacggtcg aggccggcgc gggcggccgt
     4021 gcaatccttt acacgggcgg tgtcgccggc agcaccggcg tcaccaacct cgtcggctcg
     4081 ggcagcggcc gtttccgcta caacagcgac gagtcgagca ccaactacga caaggccctg
     4141 gagagcgggc tctacgccat atatcgcgaa gcgccgaccg tcaccatccg caccaacaac
     4201 cattccaagg tttatgacgg acaggcctat accggtgcgg gcggaatcac gaccatcggt
     4261 ctgcacaatg gcgatcaact gaacgccgga acctatagcg gcggcggcat cgatgtcggt
     4321 acgtacacgc tttcatacag caccaccgac agcggcgtgg gctatgcggt gacctacgac
     4381 aacagcggca cgcttaccgt cacgccgcgt ccggtcaatg tcagcgcgaa tgcggtcacc
     4441 aagacctacg gcgatgccga tccggcgctg agctatgccg tcgagacggc atctggcggt
     4501 cgcggtctgc tggcgggcga cacgctggac ggtgccctga gccgggctgc gggcgaagat
     4561 gccggtgcgt acgccatcaa tgtcggcaca ctcgccaacg gcaactacag catcaactac
     4621 gccggcgcga atttcactat cgccaaacgt gccatcacgc tggcggccga tgccgtgagc
     4681 aagatctacg gcgaagctga tccgacgctc agcgtccgga tcaccggcgg cagcctgggc
     4741 agcgagaccg tgagcgatgc gctgagcgac gtggtgggca tgctgagccg cgcggcgggt
     4801 gagaacgtag gtgcctacga cgtggcgctg ggtaccggtg ccaaggcggc caactacgac
     4861 attagcttcg acaccgacaa caacgcgctc accattacgc cgcgtccggt cgacgtgacc
     4921 gcgattgcgg cgaacaagac ctacggtgac gccgatccgg cgctgagcta tgtcgtcgag
     4981 acgcagtccg tcggtcgcgg cctgctcgcg ggcgacgcgc tgggcggtgc gctggatcgg
     5041 gttgctggcg aggacgtcgg gacctacgcc atcggccagg gcaccttggc ggatggcaac
     5101 tacagcatca acttcaccgg cgccgaactc accattgcca agcgcgcgat cacgctggcg
     5161 gccgacgcgg tgagcaagat ctatggcgaa gccgatccga cgttcagcat cagcatcgtc
     5221 ggcggcagcc tgggcagtga gacggtgagc gatgcgctgg ccgatgtggt cggtgcgttg
     5281 agccgtgccg cgggcgagaa cgtgggcgcc tacgatgtcg cgctgggtac cggcgccaag
     5341 gcgggcaact acgacatcac gttcgatgcc gacaacgacg cgctcaccat cacgccgcgc
     5401 ccggtcgacg tcatcgcaaa tgccgcgacc aagacgtacg gcgaagccga tgtgctgacg
     5461 tacacgacgg aagcgcagac ggccggccgc ggtctgctgg cgggcgacac gctgagcggc
     5521 tcgctggacc gggccgcggg cgaggacgtc ggcacgtacg ccatcggcca cggcacactg
     5581 gagaacgtca actacagcat caatttcacc ggcgcgaacc tcaccattac gccgcggccc
     5641 gtgacggtca cggcgaacaa ccggcagaaa atgcagggca cgagcgatcc ggcgttgacc
     5701 tacgttgccg aagggcaatc cgccgggcgc ggcctcgtgc acggcgattc gctcgccggc
     5761 ctgttgagcc gggcaagcgg cgaagggctc ggcgactatg cgatcggcgc cgggtcgctg
     5821 accgatgcca acaatccgaa ttacgtcatc acgttcaagg acggcgtgtt caggatcgtc
     5881 gcggcgacgg aggtggagac ttcgcaacac gtcgcacaag ttcttccgga gaacatcacg
     5941 cagggccacg ataccgtgcc gcaaggcgat ccggcgtcgt tcggcgatca gggcagcgga
     6001 cctcagacgc agggcggcgg tctgcgcttt gtcgatgcac cggccgatga gccgagcaac
     6061 gtggggtcag gttcggatgc aggcggcacg cccggcgcag gtgattcggg tcttgcaaac
     6121 gccggtgaaa cgaccggcgg cgcgagtgac ccgggtccgc aaagcggaag cattgcgagc
     6181 agtgacggcg gtgtgaacaa cgccgacggt agcgcaagcg cggcaagcac agcggaatcg
     6241 ggcgagtccg gtaacgagga tgccggaccg gacggtcagt tcgatgtcgc ggagcgttcc
     6301 gacctggggc cgctgacggt cctcgtagtc aagggcggca taaaactgcc gagcttcgat
     6361 cggcaataac aatcaatgtc cgaaaacagt cgtcgtcccg gagcgggccg gatcgaatcc
     6421 ggtgcccgct cccagggatg atggcgctta acaagaaaac cgcaatgcat acacctatgc
     6481 gtctatctgt actcgctctt ttgctcatgg cgagcccctg catccttgcc cagacgccgc
     6541 ccgacgccgg gcgtctgctg caggatatgg aaattccgcc tcaacccatt ccgccttcca
     6601 tcgatctcaa actggaggag cgtgccccta cggatgacgc cgcgcccggc ggtccgcaag
     6661 tcgagctgag aacagttcgc ttcaacggca tgacgcgctt cgacgaagcg caactgcagc
     6721 aagtgatcgc cgatgcgatc ggccagtcgt tcgacctggc gggcctccgc aatctggccc
     6781 atcgcgtcac gcgcttttat cgcgagcagg gctatccctt cgcgcgcgcc tatctgccgc
     6841 cgcaacgcat gagcgaaggc gagctgcgca tcgaaatcct cgagggccgc tacggcaagg
     6901 tgacggcggt cggcgaaccg gaactggctg ccggtgcgca ggccttctta tcctctttac
     6961 agtccggcga ggtgatcgaa ggcaaatcgc tggaacgcgc cgcgctgatt ctcggcgatc
     7021 agccgggcgt caaactgacg ccggtcgttc gtccgggtga ggaagtgggt accggcgacc
     7081 tcgaagcgcg cgtaagtcgc ggccccgcct tcgaagcgcg cttcagtgca gacaaccacg
     7141 gcaaccgcta ttcggggcgt catcgtgcca tcgcggcagc gaactggaac agccctttca
     7201 tgctgggcga tcagttgagc gcgagcgcca tgctgaccga tgaaagccag tggttcggtc
     7261 aactcggcta tgcattgccg ctcggcgcgt cagggctgcg cggtcagatc agctatgcgc
     7321 atacctacta cgaactcggc aaggagttct ccaatctcga cgccaccggt acggcgaagg
     7381 ttgcatccgt cgggttgagc tacccgcttg ttcgtacccg tgcgcacaat ttgctgctgt
     7441 cggtgcagta tcagcacaag gatctggtcg acgaatacgg tacggtcgac gtcaagaacg
     7501 agaaatcgag cgacagcgtg ccggtcagct tgcagttcga tgcgcgcgat caactcgccg
     7561 gcggcggcat cacgtatggc gctttggcat ggaccgttgg cgatctttcg ctcgattcgc
     7621 gcgacctgcg caatcaagac cggctcactg cgaaaaccga aggcggtttc cagaagtgga
     7681 acttcgacat cgcacggctg cagcggctgc cgcagaactt cacgctgttc ggacgcttct
     7741 cggggcagtt gaccggcgac aacctcgatt cctcggagag cttcagtctc ggcggcgcga
     7801 gcggcgtgcg cgccttcccg cagggcgagg gcgcgggtga ccggggctgg ctgacgcaag
     7861 tcgaactgcg ttacacgatg ggcgagttcg cgccctatgt gttctacgac gcgggcaagg
     7921 tcaccatcaa cgccaagacc tgggacgaca gcgacaacca tcgcagcatt tccggtttcg
     7981 gtgcgggcgt gcgctggacc tgcggcaata tcggcgtgga cgcttcggtc gcatgggtcg
     8041 cgcacggcgg cgatcccgag tcggacaagc acgacacgca gccgcgcttc tggatcaagg
     8101 cggagtatcg gttgtaaggc ggtcggcagg ctcgctgcgg aggagcgact agaaaccgac
     8161 tgcgaggctg tcgcgccgca tgtcgcagca gccgacatag ccgccctcga tgcgtcggat
     8221 gatctgcgcg cagccgaagt cgtgataggc cggcggcagt tccgtcggct gatgtccgcg
     8281 gcgcgccagt tcggcgacgg tttccggcgg gaacgcttcg aagttcacct tcaatccctg
     8341 ctcgacgcgg aaacgcggcg catcgatcgc gctttgcggc gactggttgt agtcggccat
     8401 gcgcaccatc agttgcacat gaccctgcgg ctgcatggtg ccgcccatca ggccgaggct
     8461 catcagcgct tcgccatcct tcgtgacgaa gcccggaatg atggtctgga agggccgctt
     8521 gcccggcgcg acgcaattgg gtcggtccgc gcgcgtgctg aagccggcgc cgcggttctg
     8581 caggctgatg cccgtgccgg gcaccaccac gcccgagccg aagcccatgt agttcgactg
     8641 gatgaatgac accatcatgc cgtgctcgtc cgcggcggtg agataaacgg tgccgccgcg
     8701 cggcggttcg ccatggtcgt aatcctgcgc gacatcgggc cggataagtc gggcgcgctg
     8761 agcgaggtaa tcggggtcga gcagcgcgtc gggcggaaag ggcatcgcct ggggatcggc
     8821 gacatagcga tagacgtcgg cgaaggcgag cttcaccgct tcgatctgca gatgcacgct
     8881 gtccggtccg tccacgggat aagcgctcag gtcgaaatgc cgcaggatgc ccagcgcaat
     8941 gagcgcgacg atgccctggc cgttgggcgg cagttcgtgc agcgtgtagc cgcggtagtt
     9001 gaccgccagc ggctcgaccc agtcgttggt gtgcgcggcc agatcggctt cggtcatcgc
     9061 accgcgatgc gccgccgcgt gcgcggcgag gcgctcggca taaacgccgc gatagaaggc
     9121 ctcgccttgg gtctcggcga tgtgcgccag cgtgcgcgcc agcgcgggca gacggaacag
     9181 ctcgccttca tggggcgcgc ggccgcccgg cataaacgcc tccgcaaaac ccggctggtt
     9241 gtgcagatcg ggaatctgca gtgcccagcg atgcgcgatg aacggcgtca catgaaaacc
     9301 ttcgcgcgcg taatggatgc ccgcttcgaa cagttcctcg aagggcagca cgccgaagcg
     9361 ctcggacaga tcgacccacg ccgccaccgc accgggcacc gtgacgctgt tccacccgcg
     9421 cagcggaatg cgctgctgaa aatagtccgg cgtccacgcc gcgggcgcgc gccccgaagc
     9481 gttcagcccg tgcaggcgct cgccgtccca gaccagcgca aaggcatcgc tgcccacacc
     9541 gttggaaatc ggctccacca ccgtgagcgt gaccgccgcg gcgatggcgg catccaccgc
     9601 gttgccgccc ttggcgagca cggcaagacc ggcctgcgcg gcgagcggct gcgaggtggc
     9661 gacgacgttg cgcgcgaaga gcgggcggtg
//
LOCUS       DATGOU010000118         6084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3915976, whole genome shotgun sequence.
ACCESSION   DATGOU010000118 DATGOU010000000
VERSION     DATGOU010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3915976"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1043)
                     /locus_tag="VKZ48_06990"
     CDS             complement(15..1043)
                     /locus_tag="VKZ48_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009667582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HLU77629.1"
                     /translation="MDISSSAVKLVELAEVAKGGKRVERYAIEPLPRDAMLDGNIANL
                     EVVAEAVRRAWKRLATRTKHCALALPSAAVITKTIIVPAGLREQDLEIQVESEANQYI
                     PFALEEVNLDFQVVGPAPSSPEEVEVLIAASRKEKVEDRVAVAEAAGLKATIMDVESF
                     AMQTAFEMIRLQLPNEGLDQNVAVVDIGAHTTNITVLRNDEAVYTREQAFGGAQLTQD
                     IARQYALPVEEAEIAKRSGGLPDSYEAEVLRPFMENCAGEIQRAMQFFFTSTQYNSIE
                     HILLTGGTATLPGLDEIVSSRTRVNTLIANPFANMQVSPHVQLKKLMEDAPALMIACG
                     LALRRFDA"
     gene            1256..3565
                     /locus_tag="VKZ48_06995"
     CDS             1256..3565
                     /locus_tag="VKZ48_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HLU77630.1"
                     /translation="MRWVGWSLAALAGLATLGALLLVFVLLLAYPNLPSIEALADYQP
                     KVPLRVYTSDGVLIGEFGEERRAFVRIEDVPAVMKQAIVAAEDERFYTHSGVDYVGVI
                     RAAYSNLVTGDKRQGASTITMQVARNFFLSSEKTWTRKIYEVLLAFKIEAGLTKDQIL
                     EIYLNQIYLGQRAYGFAAAAQTYFGKALNDVSIAEAAMLAGMPKAPSSYNPVVNFKRA
                     KLRQLYVLRRMHELGFIDDAQLAQARDERLRVQRHVDGMPIDAQYVAEMVRQVLYERY
                     PEDVYTRGFRVYTTLRAKDQEGAVKALRTSLLEYDQRHGYRGPEAYVELPAQAGEELL
                     DEVLARYGDSGGLIAAVVLAADSKQVRAYHRGGETVTIDGAGLRFARAMLSDKAAPNK
                     RIRRGAIVRIQKDAKDRWRLVQLPEAEAAFISLDPQTGAVRSLVGGFDFWRNKYNHVT
                     QAWRQPGSAFKPFIYSAALEKGFTPATIINDAPVVVDASMTGGQAWEPKNYDGNYEGP
                     MRMRTALAKSKNMVSIRILQTIGPQYAQDYIQRFGFTPSRHPPYLTMALGAGSVTAWQ
                     MAQAYGVFANGGYRVEPYFIEKIVDDRGNVLAEAEPRRAGDESLRTIDRRNAFVMNTM
                     LQDVIREGTARRAASLGRQDLAGKTGTTNDYVDAWFAGYQPHLVGVAWVGFDQPKKLG
                     TNETGSRTALPMWIDYMRGALADIPPADLVMPEHVMAVSIDPETGLRDPSGPLTEYFY
                     EETLPPMKQVAPVEAPGSRTIEEVLDQLF"
     gene            3780..5027
                     /gene="lysA"
                     /locus_tag="VKZ48_07000"
     CDS             3780..5027
                     /gene="lysA"
                     /locus_tag="VKZ48_07000"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014192457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HLU77631.1"
                     /translation="MSEFHYRDGVLHAEAVNLVDLAERFGTPCYVYSRRALTEAYRGF
                     ATPLAGRDHLICYAMKANSNLAILDLYARLGAGFDIVSGGELERVLAAGGEPRKVVFS
                     GVGKSAGEIRRALEVGIKCFNVESGAELRRIEAIAQETQRIAPISLRVNPDVDAKTHP
                     YISTGLKENKFGVPYEEALALYRYAAQSPHLAVIGIDCHIGSQLTETAPFVEALQKVL
                     QLVDALSAEGIELHHLDLGGGLGIRYRDETPPATASYVEALLQALGDRRLTLILEPGR
                     ALVGNAGVLLTRVEYLKHNSGRDFAIVDAAMNDLMRPALYEAWHDILPVAPREGETRR
                     YDVVGPVCETGDFLGQERELALAEGDLLAVMSAGAYGMAMSSNYNTRPRAAEVMVDGE
                     AVHLIREREAVPALFAGERLLPR"
     gene            complement(4966..5649)
                     /locus_tag="VKZ48_07005"
     CDS             complement(4966..5649)
                     /locus_tag="VKZ48_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HLU77632.1"
                     /translation="MRGINLSARGIAAVKAVVFVVALLPALQLAYQALWDVEALGANP
                     AETLTRSLGDWTLRLLLLTLAVTPVRKLLGWPWLARVRRMLGLYVFFYALLHFTSYAA
                     FDQVFDFGEILRDIAERPFITVGVAALLLLMPLAATSTNGMVRRLGGANWVALHRLVY
                     VIAVLGVVHFWMMVKRDVTEPAIYAFILAVLLGYRVWEAQRKRRLLQATAAADARRRT
                     APGRLRARG"
     gene            complement(5656..>6084)
                     /gene="msrP"
                     /locus_tag="VKZ48_07010"
     CDS             complement(5656..>6084)
                     /gene="msrP"
                     /locus_tag="VKZ48_07010"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HLU77633.1"
                     /translation="PDRMPGTRLPILDWPYVEGLRIDEAMHPLTIMAVGLYGRVLPNQ
                     NGAPIRLVVPWKYGFKSGKSIVRIRFTEQQPRTAWEKANPREYGFYSNVNPDVDHPRW
                     TQRREKRLPAFFANHDTKLFNGYAEQVAHLYAGMDLRKFY"
BASE COUNT         1129 a         1971 c         1906 g         1078 t
ORIGIN      
        1 caggttgatg cgaatcatgc gtcgaacctc cgtagcgcga gcccgcaggc gatcatcaag
       61 gccggcgcgt cttccatcag cttcttcaac tgcacatgcg gcgatacctg catgttggcg
      121 aagggattcg cgatcagcgt attcacgcgc gtgcgagaac tgacgatttc atcaagcccg
      181 ggcagcgtcg ccgtcccgcc ggtgagcagg atgtgttcga tgctgttgta ttgtgtggac
      241 gtgaagaaga actgcatggc ccgctggatc tcgccggcgc agttctccat gaagggccgc
      301 agcacttccg cttcatagct gtccggcagg ccgccgctac gcttggcgat ttccgcttct
      361 tccacgggca gcgcgtactg gcgcgcgatg tcctgggtca gttgtgcgcc gccgaaagcc
      421 tgttcgcgcg tataaaccgc ctcgtcgtta cgcaggacgg tgatgttggt ggtatgcgcg
      481 ccgatgtcga ccacggcgac gttctggtcc agtccctcgt tcggcaactg cagacggatc
      541 atctcgaacg ccgtctgcat ggcgaaggat tcgacatcca tgatggttgc cttgaggcct
      601 gcggcttccg cgaccgcgac ccggtcctcg accttctcct tgcgggaggc agcgatgagc
      661 acttcgactt cctcggggct ggacggcgcg gggccgacga cctggaaatc gaggttgacc
      721 tcttccagcg cgaagggaat gtactggttg gcctcgctct ccacctggat ttcgaggtcc
      781 tgctcgcgca agccggccgg cacgatgatc gtcttggtga tgactgccgc cgaaggcaac
      841 gccagcgcac agtgcttggt gcgggtggcg agccgcttcc acgcgcggcg gaccgcttcg
      901 gcgacgactt cgagattggc gatgttgccg tccagcatcg catcgcgcgg caggggctcg
      961 atcgcatagc gctcgacacg cttgccaccc ttggccacct ccgccagctc caccagcttc
     1021 acagcggatg aactgatgtc catcccgaag atcggtggcg ccttcggttt aaagatatcg
     1081 agttgcaagc gaaacccctt tattaacaga cagttaagcg cagtttatac attttggttt
     1141 aaagcctaac aacaagcccc ggttcggtcg ctgcagtttt tcacgtttgc cgcccaaccc
     1201 gccccgccta taattcgcct tcgccttgga aagaaagaag tttccattga agtacttgcg
     1261 ttgggtcggc tggtcgctgg ccgcactggc cggtctggca accctcgggg ccctgcttct
     1321 cgtcttcgtt ctgctgctgg cctatcccaa tctcccttcc atcgaggcgc tcgccgacta
     1381 ccagcccaag gttccgctgc gggtctacac ctcggacggt gtgctgatcg gcgaattcgg
     1441 ggaggagcgc cgcgccttcg tccgcatcga ggacgtgccg gccgtcatga aacaggccat
     1501 cgtcgctgcg gaggacgaac gcttctacac ccatagtggg gtggattatg ttggtgtcat
     1561 aagagccgcg tatagcaacc ttgtcacagg tgacaagcga caaggcgcca gcaccatcac
     1621 aatgcaggtc gcccggaact ttttcctgtc cagcgagaag acctggaccc gcaagattta
     1681 cgaagtcctg ctggccttca agatcgaagc aggcctgacc aaggatcaaa tcctcgagat
     1741 ttatctgaat cagatctatc tcggccagcg cgcttacggt tttgccgccg ccgcgcagac
     1801 ctacttcggc aaggcgctga acgacgtctc cattgccgaa gcggccatgc tcgccggcat
     1861 gccgaaggcg ccctcgagct ataacccggt tgtcaatttc aagcgcgcca agctgcgcca
     1921 gctctacgtc ctgcgccgca tgcacgaact gggtttcatc gacgatgccc aactggcgca
     1981 ggcgcgcgac gagcgcttgc gcgtccaacg ccatgtcgac gggatgccga tcgacgcaca
     2041 gtacgtcgcg gagatggtgc ggcaggtgct gtacgagcgc tatccggagg acgtctacac
     2101 gcgcgggttc cgcgtctaca ccacactacg cgccaaggat caggagggcg ccgtcaaggc
     2161 gctgcgcacc agcctgctgg agtacgacca gcgccacggt tatcgcggcc cggaagccta
     2221 tgtggaactg ccggcccagg ccggagagga actgctcgac gaagtgctgg cccgctacgg
     2281 cgacagcggg ggcctgattg ccgctgtggt cctggcagcc gacagcaaac aggtgcgcgc
     2341 ctaccaccgg gggggcgaga cggtcaccat cgacggggca ggactgcgtt tcgctcgcgc
     2401 gatgctgagc gacaaggcgg cgccgaacaa gcgcatccgc cgcggcgcca tcgtacgcat
     2461 acagaaggac gccaaggacc gctggcgact ggtgcaatta cccgaagccg aggcggcgtt
     2521 catctcgctc gatccgcaaa ccggcgcagt gcgctcgctg gtcggcggct tcgatttctg
     2581 gcgcaacaaa tacaaccacg tgacgcaggc atggcgtcaa ccgggctccg ccttcaagcc
     2641 cttcatctat tcggccgcgc tggaaaaagg ctttacgccc gccaccatca tcaacgacgc
     2701 gccggtggtg gtcgatgcga gcatgacggg tggacaggcc tgggagccga agaactacga
     2761 cggcaattac gaagggccca tgcgcatgcg cacggcgttg gccaagtcga agaacatggt
     2821 ctccatccgt atcctgcaga ccatcgggcc gcagtacgcg caggactaca tccagcgctt
     2881 cggcttcacg ccctcgcggc atccgcccta tctcaccatg gcactgggcg ccggctcggt
     2941 gaccgcctgg cagatggcgc aggcttacgg cgtcttcgcc aacggaggct atcgggtcga
     3001 gccctacttc atcgaaaaga tcgtcgatga ccgcggcaac gtgctggccg aggccgaacc
     3061 gcgacgcgcc ggcgacgaat cgctgcgcac catcgatcgc cggaacgcct tcgtgatgaa
     3121 caccatgctg caggatgtca tccgggaggg caccgcccgg cgcgccgcct cgctcggccg
     3181 acaggatctg gccggcaaga ccggcaccac caacgactac gtggatgcgt ggttcgcggg
     3241 ctatcaaccg catctggtcg gtgtggcatg ggtgggtttc gaccagccga agaaactcgg
     3301 caccaacgaa acgggcagcc ggacggcgct gccgatgtgg atcgactata tgcgcggcgc
     3361 actggcggac ataccgccgg ccgatctggt gatgcctgaa catgtgatgg ccgtgagcat
     3421 cgatccggaa acgggtttgc gcgaccccag cggcccgttg accgagtact tctatgaaga
     3481 gacgctgccg ccgatgaagc aggttgcgcc ggtcgaggcc ccgggttcgc gcaccatcga
     3541 ggaagtgctg gatcagttgt tctgaacgct ggccggacga atccgctgtc cggctgcgat
     3601 cgcgccgaac ccgcttcccg tcatgtgtat gattccgtct tttgcgacac tgccatgcgc
     3661 cgcctcctgc tcatcctact gctcgccctg agcgcacaag cctgcggctc caaggggtcg
     3721 ctgtacctgc caccgccgga tgcgcaacag ccgtccgatc aaaagcagaa gtccagataa
     3781 tgtcggaatt ccattaccgc gacggcgtcc tgcatgccga agccgtcaac ctggtcgatc
     3841 tcgccgaacg cttcggcacg ccctgctacg tgtactcgcg ccgcgccctc acggaggcgt
     3901 atcgcggttt cgcgacgccg ctggccggac gcgaccacct catctgctac gcgatgaagg
     3961 ccaattccaa cctggccatt ctcgatctct acgcccgcct gggcgcgggc ttcgacatcg
     4021 tgtccggcgg cgaactggaa cgggtgctgg ccgccggggg cgagccgcgc aaggtggtgt
     4081 tctccggcgt gggcaagagc gcgggcgaga tacgccgcgc gctggaagta ggcatcaagt
     4141 gcttcaacgt cgaatcaggc gccgagttgc gtcgcatcga ggcaatcgcg caggagacgc
     4201 aacgcatcgc gccgatttcg ctgcgcgtga atccggatgt ggacgcgaaa acgcatccct
     4261 acatttccac cggcctgaag gaaaacaagt tcggcgtgcc ctatgaagag gcgctggcgc
     4321 tttatcgcta cgccgcgcag tcgccgcatc tggcagtcat cggcatcgac tgtcacatcg
     4381 gttcgcaact caccgagacg gcgcccttcg tcgaagcgct gcagaaggtg ctgcagttgg
     4441 tggatgcgct gagcgccgaa ggcatcgaac tgcatcacct cgacctgggc ggcggcctgg
     4501 gcatccgcta tcgcgacgaa accccgccgg cgacggcgag ttatgtcgag gcgctcctgc
     4561 aagcgctggg cgatcgccgg ctcacgctga tactggaacc gggccgcgca ctggtgggca
     4621 atgccggcgt gctgctcacg cgcgtggaat atctcaagca caacagcggc cgcgacttcg
     4681 ccatcgtcga tgcggccatg aacgatctca tgcgaccggc gctgtacgag gcctggcacg
     4741 acatcctgcc ggtcgctccg cgcgagggcg aaacgcgccg ttacgacgtg gtcggaccgg
     4801 tgtgcgaaac aggcgacttc cttggccagg agcgcgaact cgcgctggcc gaaggcgatc
     4861 tgctcgccgt catgtcggcc ggcgcctatg gcatggcgat gagttccaac tacaacaccc
     4921 gtccgcgtgc ggcggaagtg atggtcgacg gtgaggccgt gcacctcatc cgcgagcgcg
     4981 aagccgtccc ggcgctgttc gccggcgagc gtctgctgcc gcggtagcct gcagcaggcg
     5041 gcgcttgcgc tgcgcctccc atacgcgata gccgagcaga accgcgagaa tgaacgcata
     5101 gatcgccggc tcggtgacgt cgcgcttcac catcatccag aaatgcacga cgcccagcac
     5161 ggcgatcaca tagaccaggc gatgcagcgc gacccagttt gcaccgccca accgccgcac
     5221 catcccgttg gtcgaggtgg cggccagcgg catcagcaat aacagtgcgg cgacgcccac
     5281 ggtgatgaac gggcgctcgg cgatatcccg caggatttcg ccgaagtcga acacctggtc
     5341 gaaggctgca tagctggtga agtgcagcag cgcatagaag aacacgtaca accccagcat
     5401 tcggcgcacg cgcgccagcc acggccagcc gagcagcttg cgcaccggcg tgacggcgag
     5461 ggtcaacagc aacaggcgca gcgtccagtc gccgagcgag cgcgtcagcg tttcggccgg
     5521 attggcgccg agcgcctcga cgtcccagag cgcctggtaa gccagctgca gcgcgggcag
     5581 caatgcgacg acgaacacca ccgccttgac ggcggcgatc ccgcgtgcgg agaggttgat
     5641 gccccgcatc ggcgctcagt agaacttgcg caggtccatg cccgcgtaca agtgcgccac
     5701 ctgttcggcg tagccgttga acagcttggt atcgtggttg gcgaagaacg ccggcaggcg
     5761 cttctcccgc cgctgagtcc agcgcggatg atcgacgtcg ggattgacgt tggaatagaa
     5821 accgtattcc cgtggattgg ctttttccca ggcggtgcgc ggctgctgtt cggtgaatcg
     5881 gatgcgcacg atcgacttgc cgctcttgaa gccgtatttc cacggcacca ccagtcggat
     5941 cggcgcgccg ttctgattcg gcagcacccg cccgtacaac cccaccgcca tgatggtcag
     6001 cggatgcatc gcctcgtcga tcctcaggcc ttcgacatag ggccagtcca atatcggcag
     6061 ccgcgtgccc ggcatgcgat cggg
//
LOCUS       DATGOU010000119        10470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3464453, whole genome shotgun sequence.
ACCESSION   DATGOU010000119 DATGOU010000000
VERSION     DATGOU010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10470
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3464453"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VKZ48_07015"
     CDS             <1..489
                     /locus_tag="VKZ48_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HLU77634.1"
                     /translation="IQAALTGHLVFTTVHANNVFDVLGRFTHMGVDTYSFAAALNGVV
                     AQRLIRLNCNHCSVPVTPSEEELAASGIDASLRSSAQFRTGRGCGNCRGTGYKGRTAI
                     AEVMILTDELREMIVERAPIRTLKDTARRNGTRFLREAAVGLALSGHTSLEEINRVTF
                     VA"
     gene            470..1279
                     /locus_tag="VKZ48_07020"
     CDS             470..1279
                     /locus_tag="VKZ48_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77635.1"
                     /translation="MSPSWLEPFQRMMRRETHDILLAPYRVEFSRTRHGHPPDELVLE
                     CDTAEDRGWEFSLATLSAGLDRLEWHDAEVRIRLSNHFVRYALVPGLAALRARDERML
                     AARHQLAAIYGERAQRWQVALHDSRGASEAIAAGVDQDFLQALVAATTRAQLKLSAAE
                     PILAAAFNAHRHGIRRTPTWLCVAEPGRFALAYFEHQQWRFLQAERQRSDLGEALRPL
                     LQRACAANGASPGRVLLIADAPPKEGFGSDAGWSIEWHPLSRAAAAQEPLP"
     gene            1276..1806
                     /locus_tag="VKZ48_07025"
     CDS             1276..1806
                     /locus_tag="VKZ48_07025"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HLU77636.1"
                     /translation="MMRRLELDFLSATRPPRWPGPALLAAGLICGIAVTTAYVQANGE
                     KTLLQTQQAEARKLLRREMPQLQRPMGDPTLVAKELAHARLVLSDLARPWDAMFGELE
                     HAAHAHVALLGVQPSSAGAAVRLRGEARSYEDLLDYIARLEQSERFGSVLLASHEEQD
                     DGSVRFSLVTKWIQRQ"
     gene            1893..2333
                     /locus_tag="VKZ48_07030"
     CDS             1893..2333
                     /locus_tag="VKZ48_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77637.1"
                     /translation="MTLVVPLDQKIDGLRMEVARTQARMTGRLSPAERSDRALTSFYG
                     YFPRADSSPLWLGKIYDAAKEAGVRLASGEYEFQRVEGLRLGRYRMSLPVQGSYEQVR
                     EFVAVVLKEVPAASLDDIALRREDIGSSELEARLRLTLYMDLSQ"
     gene            2330..3013
                     /locus_tag="VKZ48_07035"
     CDS             2330..3013
                     /locus_tag="VKZ48_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77638.1"
                     /translation="MRLTSAQRWSVYAAGLAVAIGAAVWVDREGDDAAVMPSAFANAS
                     PLPPAAEAPPDDEQKHAALAIDGLPVRDYSGANGDPFSPRSWKEMAVQEAMRDAPPLP
                     PPEPQPPPLPFSYMGKLIDGKETTVFLTRNDRNYVARVGETLDSVYRVEEIGEDDMVL
                     VYLPLKHRQEMSFERDAMPVASVASRGRRAPIDDGDSPERADVEFASRPSFVDDAPRS
                     QAGPDEDDE"
     gene            3017..5377
                     /locus_tag="VKZ48_07040"
     CDS             3017..5377
                     /locus_tag="VKZ48_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HLU77639.1"
                     /translation="MRRGLVLFLLFTLYGCATPSSTDLGRELIAQGRIEEGLAVLEEA
                     AQQRDRDTAAYSLWITQRDQAVRALVREGDAQRMAGNLERAEAAYARALKLHPDATLA
                     RTGLEAVARAHRHIEQMLKADAAYRRGDIGTAESMALAVLNEDPHMRMARDLLREIAD
                     RRSSVALEPPKLGAALQKPITLEFRNAPLRSVFEVLSRTSGINFVFDRDVRSDLRATI
                     FVRDTSLDDIVKLLLITNQLNRKVLNDNTILVFPNTPAKQKDYQELVVRSFYLTNAEA
                     KQTAAMIRALVKTRDLYIDDKLNLVVMKDTPDAVRLAEQLVATQDLGEAEVVLELEVL
                     EVASNRLRDFGIRYPDQISLGVFGSGTGDSGVSNLPEFTELKPGDWDFFTVNPAVIFN
                     LRQTDGVVNTLANPRIRVKNREKARIHIGEKVPVITTTSTANVGVSSSVNYLEVGLKL
                     DVEPNVYLDDQVAIKLQLEVSNILEQLNVSGTVSYRLGTRNAATTLRLRDGETQVLAG
                     LINDEDRRAANKVPYLGDFPLLGRLFRSDNDQRVKTEIVLLITPRVVRNIVRPTHVQP
                     HVPSGTEAAPGAVPLRISATALRSLNVAGEGGGRGQGAGSAQDAAATEAPVDTGLALV
                     VNGPPEVSIGESFALSFGLPARNEGSQVPIRLTYDPAVLQLVSGASSESPGAATMQMR
                     GPTVVGSPMTVVQTQFRVIAKEPGTTAVGIESSGARDEQGQPIAIDIPSSYEVNITAG
                     AGGSAAAPVPGEAPGAPIDRPTSEPADNAPVPGADAPSPDAASDAG"
     gene            5383..5895
                     /locus_tag="VKZ48_07045"
     CDS             5383..5895
                     /locus_tag="VKZ48_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HLU77640.1"
                     /translation="MSTLGDSIARKRRQPRAGFTLIELVITVAIIGILLSVAMPFAEL
                     AVKRAKEQELRVALRQIRTALDEYKKAADQGRVTKKADESGYPPSLTILVEGVRDARA
                     PSDETMIYFMRRLPRDPFNDDPDLPAADTWGKRSYQSPPDQPEAGKDVFDVYSLSDAV
                     GINGIPYKQW"
     gene            5889..6308
                     /locus_tag="VKZ48_07050"
     CDS             5889..6308
                     /locus_tag="VKZ48_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019143024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HLU77641.1"
                     /translation="MVMRLLERRPDGTRRHGSGFTLVELLIVMAIIATLLTVALPRYF
                     GSVDKSKEVVLKQSLSVMRDALDKYYADHAKYPDELEELVEKRYIRSLPVDPLTERSD
                     TWTIIEVPPNTGLKGSVYDVRSGAEGNASDGTPYAEL"
     gene            6309..6884
                     /locus_tag="VKZ48_07055"
     CDS             6309..6884
                     /locus_tag="VKZ48_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003277590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HLU77642.1"
                     /translation="MALRQATSQPRASSGFTYIGLLILIALIGVALTAAADLTSTALQ
                     RDREQELLFIGAAYVAAIESYSRHSPAGNEFPRQLEDLIKDPRLPSLKRHIRRLYRDP
                     MSGSMDWGLVRGPNDGIVGIYSKSERAPIKQAGFPPQFNQFSSAKQYADWRFVANTLG
                     NASGDESAAAYDSGAMQRTPPPASGPISPLD"
     gene            complement(7007..8569)
                     /locus_tag="VKZ48_07060"
     CDS             complement(7007..8569)
                     /locus_tag="VKZ48_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77643.1"
                     /translation="MKQRTGTFNKTLALTVLASALAVSGAWASNGHGKGHGNGKPPAQ
                     KAEARNFQLVGYNDLQARSAYQPTIQNQDGRWIAYVGHHGGEALNSLTGQVEQNGVSI
                     VDVTNPRNPVYLHHIAGAGGAGEAGGRQMTRVCSGDVLPSGEAGKWYLLTTRGNDAHE
                     IYDVTDPANPSLLTVVVDNLNGTHKNWWDCQSGIAYLVSGVPGWSTNRHMQVFDLSDP
                     SNPVFIRNFGVAGQQPGGTPPDPNYSLHGPIKLGNRVYMGYGTSSNGLLQILDNDILL
                     TGDPTPTDENLRAPVIGEIALGPLHGAHTTMPFLNVRPPEFQDFTRGDVRNIVAISNE
                     STANQCREAHQMVYFADITTEDKPHIVSNYHVDPESAKRGDYCDVGGRFGAHSSHENF
                     NSPYGSDVLFVAYFNAGIRAVDMRNPWSPRELAYYVPAVNERTDERCVTTNGVETCKI
                     AIQTNNLEIDDRGYVYAVDRANSGMHIVRLTREAERVLDRMPEAGTPFVGENDNGKNN
                     GKKYNRKGNNHR"
     gene            complement(9210..>10470)
                     /locus_tag="VKZ48_07065"
     CDS             complement(9210..>10470)
                     /locus_tag="VKZ48_07065"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HLU77644.1"
                     /translation="IVASIVGCFFVKATGGGRIMSALYRGLIVAGILSFAAFYLITDW
                     LMADVLAGTTVDIAGTATQIGTLHLWGASVVGLALTAAMVYITEYYTATERSPVRHIA
                     AASQTGHATNIIAGLGVSMKATAWPVLSVCIAIFLSYWLAGLYGIAIAATSMLSMAGI
                     IVALDAYGPITDNAGGIAEMSNLPDSVRAITDPLDAVGNTTKAVTKGYAIGSAGLAAL
                     VLFADYTHALSIGGRHLSFDLSNHLVIIGLFIGGLVPYLFGAMAMEAVGRAAGSVVVE
                     VRRQFREIPGIMEGTAKPDYSRTVDMLMRAAIKEMIVPSLLPVLVPIAVGLLLGPQAL
                     GGVLMGSIVTGLFVAISMTTGGGAWDNAKKYIEDDNFGGKGSEAHKAAVTGDTVGDPY
                     KDTAGPAVNPLIKIINIVALLIVPLML"
BASE COUNT         2000 a         3362 c         3244 g         1864 t
ORIGIN      
        1 atccaggccg ccctcaccgg tcacctcgtc ttcaccaccg tgcacgcgaa caacgtcttc
       61 gatgtgctcg gccgcttcac ccacatgggc gtggacacct acagcttcgc cgcagcgctg
      121 aacggcgtgg tcgcccaacg cctgatccgg ctgaactgca accattgcag cgtaccggtc
      181 acgccaagcg aggaggaact cgccgcatca ggcatcgatg catcgctgcg gtcgtctgcg
      241 cagttccgta cgggccgtgg atgcggcaat tgtcgaggca ccggttacaa ggggcggacc
      301 gcaatcgccg aagtcatgat tttgacggac gagttgcgtg aaatgatcgt tgagcgtgcg
      361 cccattcgca cactgaagga cacggcacgc cgcaacggca cacgcttttt gcgcgaagcg
      421 gcagtcggac tcgcgctgag cggacacacc agcctggagg agatcaaccg tgtcaccttc
      481 gtggcttgaa ccgttccagc gcatgatgcg gcgtgaaacg cacgatattt tgctcgcacc
      541 gtatcgcgtc gagttctcgc gtacgcgaca cggccatccg cccgatgaac tcgtgctcga
      601 gtgcgacacg gccgaagaca gaggctggga attttccctc gccaccttga gcgcaggtct
      661 cgaccggctg gaatggcacg atgcggaggt gcgcatccgc ctctcgaacc acttcgtgcg
      721 ctacgcgctc gttccgggcc tggcggcgtt acgcgcacgc gatgagcgca tgctcgcggc
      781 acgtcatcaa cttgccgcga tctacgggga acgcgcgcaa cgttggcaag tcgctttgca
      841 cgactcgcgc ggcgcaagcg aggcgattgc ggctggcgtc gatcaggatt tccttcaagc
      901 gctcgttgca gcaacgacgc gcgcacaact caaattgagc gcggcagagc ccatactcgc
      961 cgctgcattc aatgctcatc gccacgggat acgcagaaca cctacctggc tctgcgtcgc
     1021 cgagccgggc cgtttcgcgc tggcctattt cgagcatcag cagtggcgct ttctgcaagc
     1081 cgaacgacaa cgcagcgatc ttggcgaggc ccttcggccg ctcctgcagc gtgcttgtgc
     1141 agcgaatggc gcatcccccg ggcgcgtgct gctcatagcg gacgcgccgc ccaaggaagg
     1201 attcggaagc gatgccggct ggtcgatcga gtggcacccg ttgagccgcg ccgcagccgc
     1261 gcaggagccg ttgccatgat gcgccgcctg gagcttgact tcctgagcgc gacgcgaccg
     1321 ccgcgttggc ccggtccggc gctgctcgcc gccgggttga tttgcggcat cgccgtcacg
     1381 accgcatacg tccaggcgaa tggagagaaa acactactgc aaacgcagca ggcggaggcg
     1441 cgcaagctgt tgcgccgtga aatgccgcaa ctgcaaaggc cgatgggcga tccgacgctg
     1501 gtcgccaaag agttggccca cgcacgactg gttctgagcg acctggcgcg cccatgggat
     1561 gccatgttcg gcgaactcga acacgccgct catgcgcatg tggcgcttct cggcgtacaa
     1621 cccagttcgg caggcgccgc agtgcgcttg cgtggcgagg cgcgcagcta cgaggaccta
     1681 ctcgactaca tcgcccgact ggagcaaagc gaacgcttcg gctccgtgct gctcgcttca
     1741 catgaggaac aggacgacgg cagcgtccgc ttctcgctcg tgaccaaatg gatacagcgg
     1801 caatgacact caagcttcat gagcgcttgc gtacgctcgg tcgatcgggc gtagccggcc
     1861 ttgcgctcct gcttctcgcc gcgctggcgt acgtgacgct cgtcgtgccc ttggaccaga
     1921 agatcgacgg gctgcgcatg gaagtcgccc gcacgcaggc gcgcatgacg ggccgccttt
     1981 cgccagcgga gcgcagcgac agggcgttga ccagcttcta cggttacttc ccgcgcgccg
     2041 acagttcacc gctgtggctc ggcaagatct acgacgccgc aaaagaagcg ggcgtcaggc
     2101 tcgcatcggg cgagtacgaa tttcaacgcg tggaaggttt gcgcctcggt cgttaccgca
     2161 tgtccttgcc ggtacagggg tcctacgagc aggtacggga atttgtcgcg gtcgtgctga
     2221 aggaggtgcc tgccgcatcg cttgacgaca tcgcgctgcg ccgcgaggac atcggcagtt
     2281 cggagctcga agcgcgattg cgtttgacgc tttacatgga tctttcccaa tgaggctgac
     2341 ttccgcgcaa cggtggagcg tctatgcggc cgggctcgcg gtcgcgattg gcgcggccgt
     2401 ctgggtcgat cgcgaaggcg acgacgccgc ggtgatgccg agcgcttttg ccaatgcctc
     2461 gcccttgccg ccggccgccg aggcgccgcc tgacgacgag cagaagcacg ccgctctggc
     2521 gatcgacggg ctgccggtaa gggactacag cggcgcgaac ggagacccgt tctcaccgcg
     2581 ttcgtggaag gagatggcgg tgcaggaagc gatgcgcgac gcaccgcctc tgccgcctcc
     2641 tgaacctcag ccgccgcccc tgcccttttc ctatatggga aaactgattg acggaaaaga
     2701 aacgaccgtt tttctcaccc gcaatgaccg gaactacgtg gcacgcgtgg gcgaaacgct
     2761 cgacagcgtc tatcgcgtcg aagaaattgg cgaggacgac atggtattgg tctatctgcc
     2821 gctcaaacac cgtcaggaga tgagtttcga gcgcgacgcc atgcccgtcg catccgtcgc
     2881 gtcccgaggc aggcgtgcac cgatcgacga cggggattcg cccgagcgcg ccgatgtcga
     2941 gttcgcctcg cgcccctcgt tcgtggacga cgcgccgcga tcgcaggccg gccccgatga
     3001 ggacgacgag taacgtatgc gccgcggcct cgtcctcttc ctcctgttca cgctatacgg
     3061 ctgtgccacc ccgtcctcga ccgatctggg gcgcgagttg atcgcgcagg gccggatcga
     3121 ggaaggtctc gcagtattgg aagaagctgc gcagcagcgc gatcgcgata cggccgcgta
     3181 ctcgctgtgg attacgcagc gcgaccaggc cgtacgcgcg ctcgtccgcg agggcgacgc
     3241 acaacgcatg gcgggaaacc tcgaacgtgc ggaggcggcg tatgcgcgtg cattgaagct
     3301 gcatcctgat gccactctcg cgcgcacggg gttggaagcg gtggcacggg cccatcggca
     3361 catcgaacag atgctgaaag cagacgccgc gtatcgacgc ggtgacatcg gcacggcgga
     3421 gagcatggct ttggcggtgc tcaacgaaga cccgcatatg cgtatggcgc gcgacctgct
     3481 gcgggagatc gccgatcggc gcagcagcgt cgcattggag ccgccgaagc tcggcgctgc
     3541 attgcagaaa ccgatcacac tggaattccg caatgcgccg ctgcgttcgg tcttcgaggt
     3601 gctgtcccgt acatcgggca tcaacttcgt tttcgatcgc gacgtgcggt ccgatttgcg
     3661 cgcgacgatt ttcgtacgcg acacttcgct ggacgacatc gtcaaactgc tgctcattac
     3721 caatcagctc aaccgcaagg tgctgaacga caacaccatc ctggtgtttc cgaacacgcc
     3781 ggccaagcaa aaggattatc aggaactggt ggtgcgcagc ttctacctga ccaacgccga
     3841 ggccaagcag accgccgcga tgatccgcgc actggtcaaa acgcgcgatc tttatatcga
     3901 cgacaagctc aatttggtcg tcatgaaaga cacgcccgac gcggtacgcc ttgccgaaca
     3961 actggtcgcc acgcaggacc tcggcgaggc ggaagtggtg ctcgagctcg aagtgctgga
     4021 ggtcgcgtcc aaccggctgc gcgatttcgg catccgctat cccgatcaga tcagcctcgg
     4081 cgtgtttggt tcgggcacag gcgacagcgg tgtttcgaat ctgccggaat tcaccgaact
     4141 caagccgggc gactgggatt tcttcaccgt caacccggca gtcattttca acctgcgcca
     4201 aacggacggc gtggtgaaca cgctcgcgaa cccgcgcatc cgcgtaaaga accgcgagaa
     4261 ggcacgtatc cacatcggcg aaaaggtgcc ggtgatcacc acgacctcga cagccaacgt
     4321 cggtgtgtcg tcctcggtga actacctcga ggtcggcctc aagctggatg tcgaaccgaa
     4381 cgtctacctc gacgaccagg tggcgatcaa gttgcagttg gaagtcagca acatcctgga
     4441 acaactcaac gtatccggca ccgtgtcata ccgtctcggt acgcgcaacg ccgcaaccac
     4501 cttgcgcctg cgtgacggcg agacgcaggt gctcgccggc ctgatcaacg acgaggatcg
     4561 gcgcgcggcg aacaaggtgc cctatctcgg cgacttcccg ctgctcggcc gcctcttccg
     4621 cagcgacaac gaccagcgcg tgaagaccga aatcgtgctg ctgatcacac cgcgagtggt
     4681 gcgaaacatt gtgcgtccga cccatgtaca accacacgtg cctagcggca ccgaggccgc
     4741 gcctggcgcc gtaccgttgc ggatcagcgc aacggcttta cgctcgctca atgtcgccgg
     4801 ggaaggcggc ggtcgcgggc aaggcgcagg ctcggcgcag gacgctgcag cgaccgaagc
     4861 gcccgtggac acgggcctgg cactcgtcgt caacggaccg cccgaagtgt cgatcggcga
     4921 aagctttgcc ctgagcttcg gcctgccggc ccgcaacgaa ggcagtcagg tgccgatacg
     4981 gctgacctac gatccggcag tactgcaact ggtgtccgga gcgagcagcg aatcgccagg
     5041 cgcggccacc atgcaaatgc gcggaccgac cgtggtcggc agccccatga ccgtcgtcca
     5101 gacgcagttt cgcgtgatcg ccaaagagcc gggcacaacc gccgtcggca tcgaaagctc
     5161 gggggctcgc gacgagcagg ggcagccaat tgcgatcgac atcccgagca gctacgaggt
     5221 caacatcacc gccggcgcag gcgggtcggc cgctgcaccc gtgcccggcg aagcgcccgg
     5281 cgcgccaatc gaccgcccga catcggagcc cgcggacaat gcccccgtgc ccggggcgga
     5341 cgcaccgtcc ccggacgccg cctccgacgc tggctaacga agatgagcac gcttggtgac
     5401 agcatcgcac gcaaacgccg ccagccccgg gcagggttca cgctgatcga acttgtcatc
     5461 accgtggcca ttatcggcat cctgctttcc gtcgctatgc cctttgccga attggcggtc
     5521 aagcgggcaa aggaacagga actgcgcgtc gcgttgcgcc agatccgcac ggctctggac
     5581 gagtacaaga aagcggccga ccaggggcgc gtcacgaaga aggccgacga gtcgggctat
     5641 ccgccttcgc tgacgatact cgtcgaaggc gtacgcgacg cgcgcgcacc gagcgacgag
     5701 acgatgattt acttcatgcg tcgcttgccg cgcgatccgt tcaatgacga tcccgacttg
     5761 ccggcggccg atacctgggg caagcgcagc tatcagtcgc cgcccgatca gccggaggca
     5821 ggcaaggacg tgttcgacgt gtactccttg tccgacgcgg tcggcatcaa cggcataccg
     5881 tacaagcaat ggtgatgcgc ttactcgaac gacgaccgga cggaacgaga cgacacgggt
     5941 ccggtttcac gctggtcgaa ctgctcattg tcatggcgat tatcgcgacc ctgctcaccg
     6001 tcgccctgcc ccgctacttc ggcagtgtgg acaagtctaa ggaagtcgtg ctcaagcaat
     6061 ccctcagcgt gatgcgcgac gcgctcgaca agtactacgc cgaccacgct aagtaccccg
     6121 acgaactgga ggaactggtg gagaagcgct acatccgcag cctgccggtg gatccgctga
     6181 ccgaacgtag cgatacgtgg accattatcg aagtgccgcc gaacacgggt ctcaagggat
     6241 cggtttacga cgtccgcagc ggcgcggaag gcaacgcctc cgacggcacg ccatatgcgg
     6301 agttatgaat ggcactgcgg caggcaacat cgcaacctcg cgcatcgagc ggcttcacct
     6361 atatcggcct gctcattctg atcgccttga tcggcgtcgc gcttacggca gccgccgatc
     6421 tgacgagcac ggcgttgcag cgggatcgcg agcaggagtt gttgttcatc ggcgcggcct
     6481 acgttgccgc gatcgaaagc tacagtcgcc actcgcccgc cggcaacgag ttcccccggc
     6541 aactggaaga tttgatcaag gatcctcggc tgccaagctt gaaacggcat atccgccgcc
     6601 tctaccggga ccctatgagc ggctcgatgg attggggcct ggtgcgcggc cccaatgacg
     6661 gtatcgtcgg gatctacagc aaatcggagc gtgcgccgat caagcaggcc ggcttcccgc
     6721 cccagttcaa tcagttctcc agcgccaaac aatatgccga ctggcgcttc gtcgccaata
     6781 cgctcggcaa tgcatcgggc gacgaaagcg ctgctgcata tgatagcggt gcgatgcaac
     6841 ggacgccgcc gcccgcaagc ggccccatca gcccgctcga ctgaaaacgg ccgctgtctg
     6901 ccggtgcgat gcggcaacgc atcgtatccc actgccttcg cgcgatgccc gaaaaaagag
     6961 ccgcggattc gtcccgcggc tcttccgtcg tgtgtttcgg cgcgactcag cggtgattgt
     7021 tccccttccg gttgtatttc ttgccgttgt tcttgccgtt gtcgttttca ccgacgaacg
     7081 gcgtccccgc ctccggcatg cgatccagca cccgctccgc ttctctcgtc agccgaacga
     7141 tatgcatgcc tgagttggcc cggtccaccg cataaacata accgcggtcg tcgatctcca
     7201 ggttgttggt ctggatcgcg attttgcagg tctcgacgcc gttggtggtc acacagcgtt
     7261 cgtcggtgcg ctcgttgacc gccggcacgt agtacgcaag ctctctcggc gaccaggggt
     7321 tgcgcatatc gactgcgcga atgcccgcgt tgaagtatgc gacgaacagt acatcgctgc
     7381 cataggggct gttgaagttt tcatgcgacg aatgcgctcc aaaccgtccg ccgacgtcac
     7441 agtagtcacc tcgcttcgca ctttccggat cgacgtggta gttggatacg atgtggggtt
     7501 tgtcctccgt cgtgatatcg gcgaaataca ccatctgatg cgcctcgcgg cattggttgg
     7561 ccgtcgactc gttcgagatc gccacgatgt tgcgcacgtc gcctctcgtg aaatcctgga
     7621 actccggagg ccgcacgttg agaaacggca tggtggtatg cgccccatgc agcggaccca
     7681 gagcaatctc gccgatcacc ggtgcacgaa ggttctcgtc ggtcggtgtc ggatcaccgg
     7741 tcaggagaat gtcgttgtcg aggatctgca gcaaaccgtt agacgaggtg ccgtaaccca
     7801 tgtatacccg gttgccgagc ttgatgggtc cgtgcaacga gtagttggga tcgggtggtg
     7861 taccgccggg ttgctgtccc gccacgccga agttgcgaat aaacaccgga ttgctcggat
     7921 cgctgaggtc gaacacctgc atgtgtcgat tggtactcca acccggcacg ccggaaacca
     7981 ggtatgcgat gccgctttgg cagtcccacc agttcttatg cgtaccgttc aggttgtcga
     8041 ccacaacagt gagcagcgac ggattcgccg ggtcggttac gtcatagatt tcgtgcgcgt
     8101 cgttgccgcg agtcgtcagg agataccact tgcctgcttc gccgctcggc agcacatctc
     8161 ccgaacacac acgcgtcatc tgccggccac ctgcttcacc cgcgccaccg gcgcctgcga
     8221 tgtgatgcag ataaaccgga ttgcgtggat tggtgacatc gacaatggac acaccgttct
     8281 gctcgacctg ccccgtcagc gaattcagcg cttcgccacc atgatggccg acgtaggcaa
     8341 tccagcgacc gtcctggttc tggattgtcg gttggtacgc gcttctggcc tgcaagtcgt
     8401 tgtaccccac gagttggaag ttgcgtgcct cggccttctg tgcaggcggt ttgccattac
     8461 cgtgaccttt gccatggccg ttgctcgccc aggcgccgga taccgcaagt gcagacgcaa
     8521 ggactgttag cgcaagtgtt ttgttgaacg tcccggttcg ctgcttcatt ggtacctcct
     8581 tcatgctgaa caaaacgtcg acacgtccgc acgggcatgc gacatcagag ccggcgcgta
     8641 tcggcactat gcaaccgagg cgcgccgcaa caagactgga tcgcgattgc ttgcgccctc
     8701 ctttcggttc accaccccgc gctgcacaat gcatacgtca cacctgcgaa cgcgttgcgc
     8761 gcttgccaaa cgacgtggta ctgatttttc tcaaaagagt gacgggatat gaagacggaa
     8821 ggacgcgctt tcgcccgctc catgtgtcgt ccccaggcga tagtggtcgc cgtccgagac
     8881 acgtgctcgc gtatgcaccg ggtgcgaaaa gcgagcttga ccatgcatgt caagcggagc
     8941 gacgcgccat ctttaatgca cgcgacttcg tcggcggagg cgttgaacgc tcgtcgctga
     9001 ttcctcgcgg tttccggagg aggcgaatga agtgaaacag agacgatccg cgtcaagcgg
     9061 tgcgacagga gaatcgcctc attggggtga agggagcagc ctgcccgagg ggcaggctgc
     9121 tttcatgcgg gagaaacaag tcctcgccgt tcaggacacg cgatttgcaa atcggagcac
     9181 tgctgcagcg agttcgcgca acaacgcact caaagcatca gcggcacgat cagcagggcg
     9241 acgatgttga tgatcttgat gagcgggttc accgccggac cggcggtgtc cttgtaggga
     9301 tcgcccaccg tatcgcccgt caccgcggcc ttgtgcgctt ccgatccctt gccgccgaaa
     9361 ttgtcgtcct cgatgtactt cttggcgttg tcccacgcgc cgccgcccgt ggtcatcgag
     9421 atggcgacga acagcccggt gacgatggag cccatcaaca cgccgccgag cgcctgcggg
     9481 ccgagcagca ggccgaccgc gatggggact agcaccggca gcagcgaagg cacgatcatc
     9541 tccttgatcg ccgcgcgcat gagcatatcc accgtgcgcg agtaatcggg cttggccgtg
     9601 ccttccatga tgccggggat ttcgcggaac tggcgccgca cctccaccac caccgaaccg
     9661 gccgcacgcc ccactgcctc catcgccatg gcgccgaaga gatacggcac caacccgccg
     9721 atgaacagac cgatgatcac caggtgattg gacagatcga agctcagatg ccgtccgccg
     9781 atactcaagg catgcgtgta gtcggcgaac agcaccaggg cagccaatcc cgccgagccg
     9841 atcgcataac ccttggtcac cgccttggtc gtgttgccca ccgcgtcgag cggatcggtg
     9901 atcgcgcgta cggaatccgg caggttcgac atttcggcga tgccgccggc gttgtcggta
     9961 atgggtccgt aggcgtccag cgcgacaatg attccggcca tggacagcat ggacgtcgcc
    10021 gcgatggcga tgccgtacag acccgcgagc cagtaggaca ggaagatggc gatgcacacc
    10081 gacagcaccg gccaggccgt cgccttcatc gacacaccca gccccgcgat gatgttggtc
    10141 gcgtgcccgg tctgcgaggc cgcagcgata tgccgcacgg ggctgcgctc ggtcgccgtg
    10201 tagtactcgg tgatgtagac catcgccgcc gtgagcgcca ggccgaccac cgacgcgccc
    10261 cacagatgca aagtgccgat ctgcgtcgcg gtgccggcga tgtcgacggt ggtgcccgcg
    10321 agcacatcgg ccatcagcca gtcggtgatg aggtagaacg cggcgaagga caggatgccc
    10381 gcgacgatca gcccacgata cagcgcgctc atgatgcgcc cgccgcccgt cgccttgacg
    10441 aagaagcagc cgacgatgga ggcgacgatg
//
LOCUS       DATGOU010000120        19871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_362514, whole genome shotgun sequence.
ACCESSION   DATGOU010000120 DATGOU010000000
VERSION     DATGOU010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 19871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19871
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362514"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VKZ48_07070"
     CDS             <1..786
                     /locus_tag="VKZ48_07070"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017334189.1"
                     /note="threonine deaminase; threonine dehydratase; in
                     Escherichia coli, IlvA is part of the isoleucine
                     biosynthetic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HLU77645.1"
                     /translation="YVDEVVLVDTDEICAAIKDVFEDTRSILEPAGALAVAGAKIYAQ
                     QTGVRDQKLVAVASGANTNFNRLRFVAERAEVGEHREALLAVTIPERPGSFKQLCAAL
                     GERSVTEFNYRYADDHAAQVFIGVQVRDRQETAKLIAQLKRQGYQPVDLSDNEMAKLH
                     VRYLVGGHAPRGANGNGVNELLYRFEFPERPGALMRFLSHMRADWNISLFHYRNHGAD
                     YGRVLVGMQVPGKEKRAFNEFLATLGYPYEDESDNPAYSLFLA"
     gene            790..975
                     /locus_tag="VKZ48_07075"
     CDS             790..975
                     /locus_tag="VKZ48_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77646.1"
                     /translation="MNDRREQLTPRLAALALTVALLAGAAIATLKGAWWTAVGFGLAW
                     LLLQCVPPVWQRLRMRR"
     gene            complement(988..1677)
                     /locus_tag="VKZ48_07080"
     CDS             complement(988..1677)
                     /locus_tag="VKZ48_07080"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HLU77647.1"
                     /translation="MSTPCNEQELRQWRKALRAELIERRMALSPETLDAHRHAMDDWL
                     ERGFPNLHRGTVAMCWPYKNEYDPRYLARKLRDRGAKTALPVVVAPRTPLLFREWHPG
                     VKLVPGVYDIPYPVESEEVIPDYVLLPMNGFDAGGYRLGYGGGFFDRTLASLKKRPVV
                     IGVTYELAKIDTIYPQPYDIPMTYVVTERGIYRRDGDKLVYLGAPEAPTDASTLSSPV
                     CYEGEAPQRRD"
     gene            1814..3757
                     /locus_tag="VKZ48_07085"
     CDS             1814..3757
                     /locus_tag="VKZ48_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HLU77648.1"
                     /translation="MRILLASLLLLPLSLYGAPDADESFLAARDAWRDSSLSKLETLL
                     PKLQGHVLEPYAQYWRLQLRIEEAAPEEVEAFLREHEGSYLAELLRKDWLRVLGKRGQ
                     WPLYDRVYAGLRTNEPDLVCYSALSRAQADPADAPPAALSAWEAASEIPEGCKPLVDA
                     LFNAGKLRTDDVWQRVRLLGAAGRGAALPQTLAYLPERERPTDKQIDRIERDPAAFLK
                     RAHKLDMRKRLNRELTIYATQRLARRDPIQAADDWTALRGRFDGRDDAYVWGQLAFHA
                     ALDHLPEALSWYERADAVALADEQVEWQARAALRARDWAALLGAVERLPKALRDESVW
                     VYWRGRALQALGHAEAATAQYERIAGQPHFYGKLALEELGRPLVVPTHRHEPSAEELR
                     AAAANPGFQRAIALFRADMWLAAVREWNWQLEGMDDRQLLAAAEHARRSELWDRAINT
                     AGRTTHLHDYWMRFLAPYRDSFAQQAASRGLDEHWILGLVRQESRFVSHARSSAGATG
                     LMQVMPATARWIARRLGINYRPGQLTDVEMNIALGTGYLEYVLDSLDGSLVMAAAAYN
                     AGPGRARRWKADHPLEGAIYIETIPFGETRNYVKNVMANTSFYAALYGGERQSLKQRL
                     GIIPPRTAGEGYAPTITGQATLQ"
     gene            3967..4914
                     /locus_tag="VKZ48_07090"
     CDS             3967..4914
                     /locus_tag="VKZ48_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HLU77649.1"
                     /translation="MANILILGGSGFIGSHLAERLIRDGHCLRVPTRRRERAKHLLVL
                     PTIDVVEANVHDPAELRALLQGMDVVINLVGVLHDGRGKPYGPGFAAAHVELPRKLVE
                     ACRATGVRRLLHMSALKAAPDAPSAYLRSKGDGEAVIVGARGTLQTTIFRPSVVFGPE
                     DNFLNLFARLQRRLPVVVLGSPDARFQPVYVGDVAEAFVRSLRTGESHGRAYDLVGPK
                     VYTLREIVRYVGEVSGYPRPILPLAPRAAYLQAMMLEFAPGKLLTRDNYYSMTVDSVS
                     DAPLPFDIDATAMEAVAPRYLADDHPRMRYDFFRYRARR"
     gene            4911..6026
                     /locus_tag="VKZ48_07095"
     CDS             4911..6026
                     /locus_tag="VKZ48_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA tRNA nucleotidyl
                     transferase/2'3'-cyclic
                     phosphodiesterase/2'nucleotidase/phosphatase"
                     /protein_id="HLU77650.1"
                     /translation="MKIYVVGGAVRDELLGRAVKDRDYVVVGATPEEMVARGFKPVGK
                     DFPVFLHPDTAEEHALARTERKTARGYTGFQFHAAPDVTLEQDLARRDLTINALARDE
                     QGNLIDPYGGLADLRAGILRHVSPAFLEDPVRVLRVARFAARFDFTVAPETMALMREM
                     AHNGEVDALVPERVWQEYAKGLMETHPVRMFEVLRECEALLKVLPEWAAVDEAHALAA
                     LRLAADADAPLAVRYAVLWFGVAPDIVAAASERLRAPNECRDLALLLARHGASLSDAA
                     RFDAAAKVSLLQQTDAFRRPERFGQLLQAFACAARARGKSVEREMACMDEALRAARAV
                     DAGSVAKAHPDRVPEAVRAARIAAVEQAATGSADGLR"
     gene            6057..7433
                     /locus_tag="VKZ48_07100"
     CDS             6057..7433
                     /locus_tag="VKZ48_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C13 family peptidase"
                     /protein_id="HLU77651.1"
                     /translation="MSLTPAPLFAGLLRNILSGARLALFMRVSPQDFRASPAHFAALF
                     FAAFAAALLSDLVKQGWPGLFNFGALPTVLSQVPVILFACLLAATLLARPALLLPLAV
                     ALIACEPLFELVGLALSSDPVGSWLGAHPGWNLALAYAFILWWLAVSMRVLVLFGGWQ
                     GARTLAAGGVFVLMTVVFVLFIPRAELWLPVRDFPADGPSVADEQVFHLQAYLLEEAL
                     GHLEPERAGRPDLYFLGVASDASQDVFVREVMAVRQLFDRRFGTAGRSLVLANSPQTL
                     ADVPIATATNLRLALERFAQVMNPQEDILFLFITTHGDAQHELAFDLPPLNLQQLRPA
                     TLARMLRESGIKWKVLVVSACYSGGFVEPLRDEDTVVITAADADSASFGCESGRDYTY
                     FGRAYFGEALRGTYSFTDAFAQARESVLARERAEDLEPSSPQIHVGAAIRPKLAQLEH
                     LLTQAGGR"
     gene            7457..8071
                     /locus_tag="VKZ48_07105"
                     /pseudo
     CDS             7457..8071
                     /locus_tag="VKZ48_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205679.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
     gene            8092..9837
                     /locus_tag="VKZ48_07110"
     CDS             8092..9837
                     /locus_tag="VKZ48_07110"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HLU77652.1"
                     /translation="MPLTVLGLSGALSHDPSAALYIDGKLVAAAEEERFVRDKHAKNR
                     MPIESARYCMAEAGIGPGDIDVVSIPYAPIPLTTPARWHYARRYWYAPDRALDAIFNG
                     NRRFRRYKQRIEWALVQLGIDPRKIELDAVEHHLTHAASAYYCSGFKEKTAILGIDGK
                     GEYATTFFGVGENGRITKIKEFYDPDSLGGLYGALTEYLGFEMLDGEYKVMGMAPYGD
                     PTRYDFSRLAKFENGELIVNTELVNVIGLRRYKQNGKGYYFSPKLVEWLGPKREGDVA
                     DEPYIHYAASMQALFEKLSLEMMDYYLGDIIRETGRLVFSGGCALNVKLNQHIIARPE
                     VKELFVQPASGDSGTAIGAAAWSTVQRGVEVEKMEHVYLGPRYTNEEVIAACANHPSR
                     PAWRRIDDVPQRIARVLVDGHPVAWFQGRMEFGPRALGARSIVGCPSVEGVADRINEQ
                     IKFRERWRPFCPSMLDTVAPQMLQSDHPAPFMTFTFPVAEEWKQRVPEVVHEDGTARA
                     QVVKREHHPRYYELLLELEKLTGNGVCLNTSLNRRGEPMICSPTDALNMFYGSDLQYL
                     IMEDIFVVKEGAAPY"
     gene            9913..10716
                     /locus_tag="VKZ48_07115"
     CDS             9913..10716
                     /locus_tag="VKZ48_07115"
                     /inference="COORDINATES: protein motif:HMM:NF018047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HLU77653.1"
                     /translation="MLQLSGAVAPYGPTLVKAGLATFEDIWQLPRNWVEPPNLRRRGW
                     SGASRLTLAGERPLPLFVKWQENYCYRSLAHPLRGRPTFHREWRNIALLRAAGIPTVD
                     PVYYGERRVQGRYQAVLLTIALDQHQELDTLFAGPVDQRLRERVLVEVGDLVRRLHEA
                     GFQHNCLGGNHIMVRHDPAGVDLRLLDLEKLKRMYRRAPAAARELARLVRHTPTLQVE
                     DHLRVLGSYMRDWPRQRRIDFVNRVNAHLVRKHQHQKRMPLTRLLPAPL"
     gene            complement(10740..11927)
                     /locus_tag="VKZ48_07120"
     CDS             complement(10740..11927)
                     /locus_tag="VKZ48_07120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HLU77654.1"
                     /translation="MPILPEARTIARAMPHHDTLPEPSSDASAHSARLVQHIREHIDE
                     AGGWIPFSQYMKLALYAPELGYYTAGSRKLGKDGDFTTAPELTPLFGQALAQQARQLI
                     DDGLTDILEIGAGSGVLAAAMLEELERLDALPERYLILELSPELRARERDTLAARVPH
                     LMERVAWLNQLPPAFSGLVLGNEVLDAMPVEIVRTADMGIEQGGVDTNLIWSFRPATA
                     QVLEAATALRLPPDYRTEIGLASQAFVRTLAEILERGAILLIDYGFPRAEYYHPQRAA
                     GTLMCHYRHRAHDDPFYLPGLQDITAHVDFTAMAEAAHAGGLEVAGYTTQAHLLINLG
                     ITDLLMRIPPDDAQRYLPAAAAVNKLMSPAEMGELFKAVAFTRDIDRPLLGFRSGDRR
                     HTL"
     gene            11955..12704
                     /locus_tag="VKZ48_07125"
     CDS             11955..12704
                     /locus_tag="VKZ48_07125"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF006598.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HLU77655.1"
                     /translation="MSQNLSGKVVLVTGGAKRIGAAIARQFHASDARLMLHYHTARAE
                     AQVLQAELNGVHPESVATVQADLNRSAALPEIVEAAVTRFGRLDVVVNNASTFYPTPV
                     GSITEEAWNDLMGVNVRAPLFLSQAAAPYLRKTGGCIVNIVDIHADRPLKDYVVYSVA
                     KAALVGLTRSLARELGPEVRVNAVAPGPVLWPDSHAFDELAQQRIVSHTVLKRVGAPE
                     DVARAVVFLAAEAPYVTGQVLAVDGGRSINL"
     gene            12717..13616
                     /gene="ttcA"
                     /locus_tag="VKZ48_07130"
     CDS             12717..13616
                     /gene="ttcA"
                     /locus_tag="VKZ48_07130"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HLU77656.1"
                     /translation="MNAPASKKMQKAAYEANKLQKRLRRQVGQAIADYDMIRAGDRVM
                     VCLSGGKDSYGLLDILMRLREAAPLDFEIVAVNLDQRHPGFPEHVLPDYLRALGVPFR
                     IEVQDTYSIVKRVIPEGKTMCSLCSRLRRGVLYRVAGELGATKIALGHHRDDILGTFF
                     LNLFFGGKLKAMPPKLVSDDGRHVVIRPLAYVKEADLEAYATARQFPIIPCDLCGSQD
                     TLQRQQVKAMLRDWEKRFPGRVESIFRSLTDVRLSHLLDRERFDFTAVAADGRPSADG
                     DRAFDPEDFAAPAAVPLYLRDAD"
     gene            13769..15265
                     /locus_tag="VKZ48_07135"
     CDS             13769..15265
                     /locus_tag="VKZ48_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HLU77657.1"
                     /translation="MFGSQAEVARRLKLDRAVVNNWVKSGYVPSRWAMEIERLTEGQI
                     AAIEVLNEANARKPIRVKSRGEDESLFGPTFVGSATMNPYAPHKRIQSFHPPQRALLG
                     PGPSEINPRVMAAMGLPAIGYLDPIFVEMMEDLKSLLRYVYQTKNALTFPISGPGSVG
                     MEYCFVNMVAPGDTVIVMRNGVFGGRMIENVERCGAKPIVVEDEWGEPCDPNKLEDAL
                     KKHPEARVVAFVHAETSTGVASDAKTLIDVAHRHDALTIMDAVTSLGGSPVLVDEWQC
                     DAVYSASQKCLSCTPGLSPVTFSDRVVDYVKSRKDKIRSWFMDMNLLLGYWGNTTRTY
                     HHTAPTNSLYALHEALLILKEEGIENAWARHQRHHTALKAGLESMGLQYLVKEGYRLP
                     QMNAVYIPESLLEVEAEVRKRLLNEFNLEIGAGLGPLAGKIWRIGIMGYSCSSSNVML
                     CLSALGSVLEDIGYPIQVGDAEGAAHQAYAQMHAKAAIQKKRHTAAAA"
     gene            complement(15316..15708)
                     /locus_tag="VKZ48_07140"
     CDS             complement(15316..15708)
                     /locus_tag="VKZ48_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLU77658.1"
                     /translation="MQEDDLPYAAIVRNISEAFIYADRKGIIRWWSEGAEKLFGFTKE
                     EALGKSLDLIIPENLRDGHWRGFNAAMETGDTKYHGRIMRTRSLHKTEEKIYVEMSFH
                     LAKDEDGKVLGSSAIARLAPPKQPAQPQ"
     gene            complement(15730..16596)
                     /locus_tag="VKZ48_07145"
     CDS             complement(15730..16596)
                     /locus_tag="VKZ48_07145"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HLU77659.1"
                     /translation="MSTAILATRKLLQQRDPRVLLALALGGVAIFFLLQTALSGAASA
                     QALRPALLATLFTALATGAGALPVLFVGQVSMRTHSAMLGFGAGVMLAAAAFSLAVPA
                     LSLSSPTVVALGILLGAGVMLAIDRFLPHAHADQPTRRPYAMARVWLVVFAIALHNVP
                     EGLAVGVAYAGGGAQGTAVTLGIGIQNLPEGLIVAWAMLALGYGRARSAVVALATGLM
                     EPIGALLGVAMIGVSAALLPWGLAFAAGAMIFVVSHEIIPESHRLGHQSHATAGVTAG
                     FAIMLLLNGALA"
     gene            16823..17746
                     /locus_tag="VKZ48_07150"
     CDS             16823..17746
                     /locus_tag="VKZ48_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77660.1"
                     /translation="MAATDRAGLLKLALMAACADPAPDASAQAQLRQVAEQLSMGAGE
                     NLALALRDILLGRTQIEQLAEPLRRPEVALIAYEMAVCICSADSVHSDPEHAFLERLR
                     IALAMDVRTARSFERTADAVTGVPLQADPGRVPLATGHPVDESLLVRLVDDYAVLTAA
                     LAARAEPLALLATVPIDMKMLYRIGKYYGQELERGQVSAFMCAAGAEPVAQYVEACGQ
                     LIIAGAMDRPRARSLSYDGVAAHCYARTSALGRLARLWCSEGEHNDALLQERYTSWYA
                     EAQQGYEARRTEVAERARQTAVNDLLTLVKQ"
     gene            complement(18088..>19871)
                     /locus_tag="VKZ48_07155"
     CDS             complement(18088..>19871)
                     /locus_tag="VKZ48_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLU77661.1"
                     /translation="RSDTADELPLTQRIRGEQIRLVFLHSPTTTLASLIAALCLVWIV
                     YDEVPTYLWLGWLGAMCVHQMVRVLHYRRFVAANPRGGDLEQWGQRYLYAITTAGALW
                     GSAGVLLYVPDSATSQTYLAMLLFCIVAVSTGSLAIFAPAFYMLAALVTPPFIVRAFI
                     GGSAHEIALGVPVAIGTLAALSFGRRVNQLVHESIRRRFENVELINELKRQKQLADTA
                     RMQAEAANRSKTQFFAAASHDLRQPLHAMGLFAAALSEKIRDPEVLNVVNSINASVDA
                     LEGLFNELLDLSKIDAGVIRAELTDFPVSRVLDRLRLEFEPEAFEKGLRLHVRKSDAV
                     AHSDPILVERILRNLIANAIRYTSAGGIVVACRMRQSKLSMEVWDTGMGVSREERERI
                     FDEFYQIGNPERSSRKGLGLGLSIVRRLVRLLGTDIELTSEVGRGTRFRFELPRGRAA
                     RLMRSAQQRGAGQAADLQGRLIVVIDDESTIVEGMRVLLAGWGAEVIGSTTGEDVVDR
                     IHQVGRLPDLIIADYRLADAETGTQLIERLRRDLDPEIPAILITGSTTPERVEEAKQY
                     GYHLLVKPVLPAKLRTLINFKLQNASV"
BASE COUNT         3448 a         6312 c         6525 g         3586 t
ORIGIN      
        1 tacgtggacg aagtcgtgct ggtcgacacc gacgagattt gtgccgcaat caaggacgta
       61 ttcgaggata cgcgctccat tctggagccg gccggcgcgc tggccgtcgc gggcgcgaag
      121 atctacgctc agcagacggg cgtgcgcgac cagaaactgg tcgccgtcgc cagcggcgcc
      181 aacaccaact tcaaccgctt gcgcttcgtc gccgagcgcg ccgaggtggg cgaacaccgc
      241 gaggcgctgc tggccgtcac gatcccggag cgtccgggca gcttcaagca actgtgcgcc
      301 gcgctgggcg agcgcagcgt gacggaattc aactaccgct atgcggacga tcacgccgcg
      361 caggtcttca tcggcgtgca ggtgcgcgac cgtcaggaaa ccgccaagct catcgcgcaa
      421 ctcaaacgcc agggctatca acccgtcgac ctgtcggaca acgaaatggc caagctgcat
      481 gtgcgctatc tggtcggcgg acatgcgccg cgcggcgcca acggcaacgg cgtgaacgag
      541 ttgctctacc gcttcgaatt tcccgagcgg ccgggcgcac tgatgcgctt cctctcgcat
      601 atgcgtgcgg actggaacat cagcctgttc cattaccgca accacggtgc cgactacggc
      661 cgcgtgctgg tcggcatgca ggtgccgggc aaggaaaagc gcgctttcaa cgaattcctc
      721 gccacactgg gctatcccta tgaggacgag agcgacaacc cggcctacag cctgttcctc
      781 gcctgaccga tgaatgatcg ccgcgagcag ttgaccccgc gcctcgctgc gctcgcgctc
      841 accgtcgccc tgcttgccgg cgcggccatt gcgacgctca agggcgcgtg gtggactgca
      901 gtggggttcg gactggcgtg gctgctgctg cagtgcgtac cgccggtgtg gcaacgcctg
      961 cggatgcggc gatgagcgcc gcgcggttca gtcgcgccgt tgcggcgctt cgccttcgta
     1021 gcagaccggc gaactgagtg tgctggcgtc ggtcggcgcc tcgggtgcgc ccagatagac
     1081 cagcttgtcg ccgtcgcggc gatagatgcc gcgttcggtg accacatagg tcatcggaat
     1141 gtcgtaaggc tgcggataga tggtgtcgat tttcgccagt tcgtaggtca cgccgatcac
     1201 caccggccgt ttctttaggc tggcgagggt gcgatcgaag aagccgccgc cgtagccgag
     1261 ccggtagccg ccggcgtcga acccgttcat gggcagcagg acgtagtccg gaatgacttc
     1321 ctcgctttcc accgggtagg ggatgtcgta cacgccgggg accagcttca caccaggatg
     1381 ccattcgcgg aacagcaggg gcgtgcgcgg cgcgaccact acgggtagcg ccgtcttggc
     1441 gccgcggtca cgcagcttgc gcgccagata gcgcgggtcg tattcgttct tgtacggcca
     1501 gcacattgcc accgtgccgc gatgcagatt gggaaagccg cgctcgagcc agtcgtccat
     1561 cgcgtggcga tgagcgtcca gcgtttctgg tgacagcgcc atgcgacgct cgatcagttc
     1621 ggcgcgcagg gccttgcgcc attggcgcaa ctcctgttcg ttgcaaggtg tgctcatttt
     1681 ttcgatttcc tgattgtcga ttccctgatc ctggcgtgga caaacgcagg gtttggcgca
     1741 ggatgtgaaa gagcgtgaat gcgactgcca ttgttcacgc aaacgcggag aggcgcgggc
     1801 taatatagca cccatgcgca ttctcctcgc ctcgctcctg ctgttgcccc tctcgttgta
     1861 cggggcgcct gatgcggacg aatccttcct ggccgcccgc gatgcgtggc gcgacagttc
     1921 gctctccaag ctcgaaaccc tattgccgaa gttgcagggg cacgtgctgg aaccctacgc
     1981 gcagtactgg cgtctgcagc tccgtatcga ggaggccgca cccgaagagg tcgaggcgtt
     2041 cctgcgcgaa cacgaaggca gctacctggc cgaactgctg cgcaaggact ggctgcgggt
     2101 gctcggcaag cgcgggcagt ggccgctgta cgaccgggtc tatgcgggcc tgcgcacgaa
     2161 cgagccggat ctcgtctgct atagcgcgct gtctcgggcg caagccgatc ccgccgacgc
     2221 accgcctgcc gccctctccg cctgggaggc ggcgagcgaa attccggaag gctgcaaacc
     2281 gctggtcgat gcgctgttca acgccggcaa gctccgtacg gacgatgtct ggcagcgcgt
     2341 gcgcctgctg ggcgcggccg ggcgcggtgc cgcattgccg cagacgcttg cttatctgcc
     2401 cgagcgcgaa cggccgaccg acaagcagat cgatcgcatc gaacgcgacc cggccgcctt
     2461 tctcaagcgc gcgcacaaac tcgatatgcg caagcggctc aaccgcgaac tgaccattta
     2521 cgcgacgcag cgccttgcgc ggcgcgatcc tattcaggcc gccgatgact ggaccgctct
     2581 gcgcggccgc ttcgatgggc gggacgacgc ctacgtgtgg ggtcaactcg cctttcacgc
     2641 cgcgctcgat catctgcccg aggcgttgag ctggtacgag cgcgccgacg cggtggcgct
     2701 ggccgatgag caggtcgaat ggcaggcacg agcggcgctg cgcgcccgcg attgggccgc
     2761 cctcctcggc gccgtcgagc gcttgcccaa ggcgctgcgt gacgaatcgg tgtgggttta
     2821 ttggcgcggc cgcgccctgc aggcgcttgg ccatgccgag gccgccacgg cgcagtacga
     2881 gcgcatcgcc ggccagccgc atttctacgg caagctggcg ctggaagaac tcggccgtcc
     2941 gctggtggtg ccgacgcatc gccatgagcc cagcgccgag gaattgcgcg ccgccgccgc
     3001 caatcccggc ttccagcgcg ccatcgcgct gttccgcgcc gacatgtggc tggccgccgt
     3061 gcgcgagtgg aactggcaac tggaaggcat ggacgaccgc caactgctgg ccgctgccga
     3121 acatgcacgg cgctcggagc tgtgggatcg tgccatcaac accgccggcc gcacgacgca
     3181 tctgcacgac tactggatgc gttttctggc gccctatcgc gacagcttcg cccagcaggc
     3241 cgcatcgcgc gggctggacg agcattggat cctgggactg gtgcggcagg aaagccggtt
     3301 cgtctcgcac gcccgttcgt cggccggcgc gaccggtctg atgcaggtga tgccggccac
     3361 ggcgcgctgg attgcgcgtc ggctgggcat taactatcga cccggccaac tgaccgacgt
     3421 cgagatgaac atcgcgctcg gcaccggcta tctggaatat gtgctcgact cgctggacgg
     3481 cagtctggtg atggccgccg cggcctataa cgccggcccc ggccgcgccc ggcgctggaa
     3541 ggcggaccat ccgctggaag gcgcgatcta catcgaaacc attccgttcg gcgagacgcg
     3601 caactacgtc aagaacgtga tggccaacac ctccttctat gccgcgctct atggcggcga
     3661 gcgccagagc ctcaagcagc gtctgggcat cattccgccg cgcacggcgg gcgaaggcta
     3721 cgcgccgacg atcaccggtc aggcgacgct acaatagggc ggtttgcgcg atgcggcgca
     3781 cgcgtgccgc gtctcatgat tgcggccgca gcggccgcgc cgcccttatt ggtgaagcga
     3841 tgatcaagac catggttgct acatgtgctg tgggcatcgc cgcatccgcg gcgtgtcgca
     3901 cgacccgacg cccgcagcat gcgggcgtcc gcacttccac gcgctcccac gcccgtgagg
     3961 tgagtcgtgg ctaatatcct gatcctcggt ggcagcggct ttataggcag ccacctggcg
     4021 gagcgcctga tccgcgacgg tcattgcctg cgggtgccga cgcgccgtcg cgaacgcgcc
     4081 aagcacctgc tggtgctgcc caccatcgac gtggtcgagg cgaacgtgca cgacccggcc
     4141 gaactgcgcg cgctgctgca gggcatggac gtggtgataa acctggtcgg cgtgctgcac
     4201 gacggacggg gcaagcccta cgggcccggc tttgccgccg cgcatgtgga attgccccgc
     4261 aaactggtgg aagcctgccg cgccaccggc gtgcggcgcc tgctgcacat gagcgcgctc
     4321 aaggcagcgc ccgatgcgcc gagtgcctat ctgcgctcca agggcgacgg cgaggcggtc
     4381 atcgtcggcg cccgcggcac gctgcagacc accatcttcc gcccgtcggt cgtgttcggg
     4441 ccggaggata acttcctcaa tctcttcgcc cgactgcagc gcaggctgcc ggtggtggtg
     4501 ctcgggtcgc cggatgcgcg cttccagccg gtgtatgtcg gcgatgtggc cgaggcgttc
     4561 gtgcgcagcc tgcgcaccgg cgagagccat ggccgcgcct acgatctggt cgggcccaag
     4621 gtctatacct tgcgcgagat cgtgcgctat gtcggcgagg tgtccggcta tccgcgcccg
     4681 atcctgccgc tggcgccgcg cgccgcttac ctgcaggcga tgatgctgga gttcgcgccg
     4741 ggcaagctgc tcacgcgcga caactactac tccatgacgg tggacagcgt ttcggatgcg
     4801 ccgctgccct tcgacatcga tgccacggcg atggaagcgg tggcgccgcg ctatcttgcg
     4861 gacgaccatc cgcgcatgcg ttacgacttt ttccgctacc gggcgcgccg gtgaaaatct
     4921 atgtggtggg cggcgcggtg cgcgacgagc tgctcggccg cgccgtcaag gaccgcgact
     4981 atgtggtcgt gggcgccacg ccggaggaga tggtcgcgcg cggcttcaag ccggtcggca
     5041 aggacttccc cgtattcctt catccggaca ctgcggagga gcatgcgctt gcgcgcaccg
     5101 agcgcaagac cgcgcgcggc tacaccggct tccagttcca tgcggcgccg gatgtgacgc
     5161 tcgagcagga tctggcgcgc cgcgatctca ccatcaacgc gttggcgcgg gacgagcagg
     5221 gcaatctcat cgatccgtac ggcggtcttg ccgatttgcg cgccggcata ttgcgccacg
     5281 tcagcccggc ttttctggaa gacccggtgc gcgtcctgcg tgtggcgcgc ttcgccgccc
     5341 gcttcgattt caccgtcgcg cccgaaacca tggcgctgat gcgcgaaatg gcgcacaacg
     5401 gcgaagtcga tgcgctggtg cccgagcgcg tgtggcagga gtacgccaag ggcctgatgg
     5461 agacgcatcc tgtgcgcatg ttcgaggtgc tgcgcgaatg cgaggcgctg ctcaaggtgt
     5521 tgcccgagtg ggccgcggtc gacgaggcgc atgcgctggc cgcgctgcgg cttgcggcgg
     5581 acgccgatgc gccgcttgcg gtccggtatg ccgtgctgtg gttcggcgtc gcgccggaca
     5641 tcgtcgctgc cgccagcgag cggctgcgtg cgccgaacga atgtcgcgat ctcgcgctgc
     5701 tgctggcccg gcatggggct tcgctgtccg atgcggcgcg cttcgacgcc gcagcgaaag
     5761 tgagcctgct gcagcagacg gatgccttcc gtcggccgga gcgcttcggc cagttgctgc
     5821 aggcgttcgc ctgcgccgcc cgagcgcgcg gcaaatcggt cgagcgcgag atggcgtgca
     5881 tggacgaggc gctgcgcgcg gcgcgcgcgg tggacgcggg ttccgtggcc aaggcccatc
     5941 cggaccgggt gccggaagcg gtgcgcgcag cgcgcattgc cgcggtggaa caggctgcga
     6001 ccgggtccgc cgacgggctg cgctaaaatc gcccgctgtt tcccgatccc ttccccatgt
     6061 ctctgactcc agcaccgctg tttgcgggcc tgctgcgcaa tatcctgagc ggcgcccgtc
     6121 tggcgctttt catgcgcgtg tcgccgcagg acttccgggc gtcgccggcg catttcgccg
     6181 cgctgttctt cgccgctttc gcggccgcgc tgctgagcga tctcgtcaag cagggctggc
     6241 ccggtctgtt caatttcggc gcgctgccga ccgtattgag ccaagtgccg gtgattctgt
     6301 tcgcatgcct gctggcggcc accctgctgg cgagaccggc gttgctgctg ccgttggcgg
     6361 ttgcgttgat cgcctgcgag ccgttgttcg aactggtggg cctggcgctg tcatccgatc
     6421 ccgtcggttc gtggctcggt gcgcacccgg gatggaatct cgccctcgcc tacgccttca
     6481 ttctgtggtg gctcgccgtc tcgatgcggg tgctggtgct gttcggcggc tggcagggtg
     6541 cgcgcacgct cgccgcaggc ggcgtcttcg tgctcatgac ggtcgtgttc gtgctgttca
     6601 tcccgcgtgc cgaactgtgg ctgccggtcc gcgactttcc cgcagacggc cccagcgtgg
     6661 ccgacgagca ggtgttccat ctgcaggcgt atctgctgga ggaagcgctg ggccacctcg
     6721 aaccggaacg cgccggccgg ccggaccttt attttctggg cgtcgcgtcc gatgcgtcgc
     6781 aggatgtgtt cgtgcgcgaa gtcatggcgg tgcgccagtt gttcgaccgg cgcttcggca
     6841 cggcgggccg ctcgctggtg ctggccaaca gtccgcagac gctggccgat gtgccgattg
     6901 ccacggccac caatctgcgc ctggcgctgg agcgcttcgc ccaggtgatg aacccgcagg
     6961 aagacattct gttcctgttc atcacgacgc acggcgacgc gcagcatgag ctggctttcg
     7021 atctgccgcc gctcaacctg cagcaactgc ggccggctac gcttgcgcgc atgctgcgcg
     7081 aaagcgggat caaatggaag gtcctggtcg tgtcggcctg ttattcgggg gggttcgtcg
     7141 aaccgctgcg cgatgaggac acggtggtga tcaccgccgc ggatgccgat agcgcttcgt
     7201 tcggctgcga gagcggtcgc gattacactt acttcggtcg cgcgtatttc ggcgaggcgt
     7261 tgcgcgggac atattcgttc accgacgctt tcgcgcaggc gcgcgaaagc gtacttgcgc
     7321 gcgagcgcgc cgaagacctc gagccttcca gcccgcagat acatgtcggc gcggcgatcc
     7381 ggccgaaact tgcgcaactg gagcatctct tgacccaagc cggcggccgt tagctgaccg
     7441 ttaacgtgga ggaagaatga tcgatcttta cacctggaaa acgccgaacg gccgtaagat
     7501 ttccatcatg ctcgaagaaa ccgggctgcc gtatcgggtg catacgatcg atatcggcaa
     7561 gggcgaacag tttgcgccgg acttcctcaa gatcagcccg aacaacaaga ttcccgccat
     7621 cgtcgatccc tacggaccgg acggcgagcc gatttcgctg ttcgagtcgg gcgccatcct
     7681 gatttatctc tccgcgaaag ccggcaaact gctgcccgag ggcatgcgcg ggcgcttcga
     7741 ggcgctgcaa tggctgatgt tccagatggg cggcgtgggc ccgatgttcg ggcaggtgca
     7801 tcacttcctg cgctttgccg gcgaggacgt gccgtacgcg aagaagcgct acaccgacga
     7861 gaaggatcgt ttgtacggcg tgctcgaccg gcgactgggc gaggccgagt tcctggcgga
     7921 cgaatattcg atcgccgaca tcgccaccta tccctgggtc gcgcgccacg aatggcaggc
     7981 gaccgatctc aacgactatc ccaacgtcag gcgctggttc gacgccatca gtgcgcgacc
     8041 ggccgtacag cgcggcatgg ccgtgcccgc gtagccacaa ggtaggacat catgccgtta
     8101 accgttctgg gcctgtccgg ggccctgagt cacgacccat ccgccgcgct ctacatcgac
     8161 ggcaagctcg ttgccgcggc ggaagaggaa cgcttcgtgc gcgacaagca cgccaagaac
     8221 cgcatgccga tcgagtcggc gcgctattgc atggcggagg cgggcatcgg gccgggcgac
     8281 atcgacgtgg tatccattcc ctatgcgccg attccgctca cgacgcccgc gcgctggcat
     8341 tacgcgcggc gttactggta tgcgcccgat cgcgcactcg atgccatttt caacggcaac
     8401 cgccgcttcc gccgttacaa gcagcgcatc gaatgggcac tggtgcaact gggcatcgac
     8461 ccgcgcaaga tcgaactcga cgcggtggag caccacctga cgcacgcggc gagcgcctac
     8521 tactgcagcg gcttcaagga gaagaccgca atcctcggca tcgacggcaa gggcgagtac
     8581 gccacgacct tcttcggcgt gggcgagaac ggcaggatca ccaagatcaa ggagttctac
     8641 gacccggatt cgctcggcgg gctgtacggc gcgctgaccg agtatctggg cttcgagatg
     8701 ctcgacggcg agtacaaggt catgggcatg gcgccctacg gcgatccgac ccgttacgat
     8761 ttttcgcgcc tggcgaagtt cgagaacggc gaactgatcg tcaacacgga actcgtcaac
     8821 gtcatcggcc tgcgccgcta caagcagaac ggcaagggct attacttctc gcccaagctg
     8881 gtcgagtggc tgggaccgaa gcgcgaaggc gatgtggcag acgagcccta catccactat
     8941 gcggcgagca tgcaggcact gttcgagaag ctgtcgctgg aaatgatgga ctactacctc
     9001 ggcgacatca ttcgcgagac gggtcgtctg gttttctcgg gcggctgtgc gctgaacgtg
     9061 aagctcaacc agcacatcat cgcgcgcccg gaagtgaagg aactcttcgt gcagcccgcg
     9121 tcaggcgatt cgggcacggc catcggtgcg gccgcctggt ccaccgtgca gcgcggcgtc
     9181 gaagtggaga agatggaaca cgtctatctc gggccgcgct ataccaatga agaggtgatt
     9241 gccgcgtgcg cgaaccatcc ttcgcgtccg gcctggcgca ggatcgatga cgtgccgcag
     9301 cgcattgcgc gggtcctcgt ggacgggcat ccggtcgcct ggtttcaggg ccgcatggaa
     9361 ttcggcccgc gcgcgctggg tgcgcgttcc atcgtgggtt gtccgtcggt cgagggcgtg
     9421 gccgatcgca tcaacgaaca gatcaagttc cgcgagcgct ggcgaccctt ctgcccgtcg
     9481 atgctcgata cggtggcgcc gcagatgctc cagtccgacc atcccgcgcc gttcatgacc
     9541 ttcactttcc cggttgcgga ggaatggaag cagcgcgtgc cggaagtggt gcacgaggac
     9601 ggcaccgcgc gtgcgcaagt ggtgaagcgc gagcaccatc ctcgttacta cgaattgctg
     9661 ctggaactgg aaaagctcac cggcaacggt gtgtgcctca acacttcgct caaccgccgc
     9721 ggcgaaccga tgatctgctc gccgaccgac gcgctgaaca tgttctacgg ctcggatctg
     9781 cagtacctga tcatggagga catttttgtc gtcaaggaag gtgcggcacc ctactgagca
     9841 aggcaaaagc gcccccgacg catgatttca tgctagaagg aggccatata agttgaaata
     9901 cgtttgcaag acatgctgca gttgtccggc gcggtcgcgc cttacgggcc gacgctcgtc
     9961 aaagcgggcc tggccacttt cgaagacatc tggcaactgc cgcgcaattg ggtcgaaccg
    10021 ccgaacctgc gccggcgcgg ctggagcggt gcgagccgat tgacgcttgc cggcgagcga
    10081 cccctgcccc tattcgtgaa gtggcaggaa aactactgct atcgctcgct cgctcatccc
    10141 ctgcggggac gacccacgtt ccaccgcgaa tggcgcaaca tcgccttgct tcgcgccgcc
    10201 ggcatcccga ccgtcgatcc ggtgtattac ggcgagcggc gcgtgcaggg ccgttatcag
    10261 gcggtactgc ttaccatcgc gttggaccag caccaggaac tggacacgct gttcgccgga
    10321 ccggtcgatc aacgcctgcg cgagcgcgta ctggtcgagg tgggcgatct ggttcggcgc
    10381 ctgcacgaag caggcttcca gcacaactgt ctgggcggca atcacatcat ggtccggcac
    10441 gatccggccg gcgtcgatct ccggctgctc gatctggaaa aactcaagcg catgtatcgt
    10501 cgggcgcctg cggccgcacg cgaactggcg cgccttgtcc gccatacgcc gacgctgcag
    10561 gtcgaagacc acctgcgcgt gctcggcagc tatatgcgcg actggccgcg tcagcggcgc
    10621 atcgatttcg tgaatcgcgt caatgcgcat ctggtacgca agcaccagca ccagaaacga
    10681 atgccgctca cccgacttct gccggcgccg ctataagacg catgtttccg cgtttgcgct
    10741 tacagcgtat ggcggcgatc gcccgagcgg aagccaagta gcggccggtc gatgtcccgt
    10801 gtaaaggcga cggccttgaa cagctcgccc atttccgcag gcgacatcag tttgttcact
    10861 gccgctgcag caggcagata gcgctgcgca tcgtccggcg ggatgcgcat cagcagatcc
    10921 gtgatgccga gattgatgag caggtgcgcc tgcgtggtgt agcccgcgac ctcgaggccg
    10981 cccgcatgcg ccgcttcggc catggcggtg aaatccacgt gcgcggtgat gtcctgcaac
    11041 ccgggcaggt agaacggatc gtcgtgcgca cgatgccggt aatggcacat caaggtgccg
    11101 gcggcgcgct gggggtggta gtactcggcg cgcgggaaac cgtagtcgat cagcagtatt
    11161 gcgccgcgtt cgagtatttc ggccagtgtc cgcacgaacg cttgcgaggc gaggccgatt
    11221 tccgtgcggt aatcgggcgg cagtcgcaac gcggtagccg cttcgagcac ctgagcggtg
    11281 gcgggacgga acgaccagat caggtttgta tcgacaccac cctgctcgat tcccatatcg
    11341 gccgtgcgga cgatttccac cggcatggca tccagcactt cgttgccgag tacgaggccg
    11401 ctgaatgcgg gcggcaactg attgagccag gctacacgct ccatcagatg cgggacgcgc
    11461 gccgccagcg tgtcgcgttc acgcgcacgc agttccggcg acagctcgag gatgaggtaa
    11521 cgctcgggca aggcgtcgag gcgttcgagt tcttccagca tcgcggcggc caatacgccg
    11581 ctgcccgcac cgatttcgag gatgtctgtc aacccgtcgt cgatgagctg gcgcgcctgc
    11641 tgcgccagtg cctgcccgaa caggggcgtc agctcgggcg ccgtggtgaa gtcgccgtcc
    11701 ttgccgagct tgcggctgcc ggccgtgtaa tagcccagtt ccggcgcata cagcgccagt
    11761 ttcatgtatt gagaaaacgg tatccagccg ccggcttcgt cgatgtgttc gcggatgtgt
    11821 tgcacgagcc gcgcgctgtg tgcgctggcg tcgctggagg gttcgggaag cgtatcgtgg
    11881 tgaggcatgg cgcgggcgat ggtgcgcgcc tccggtagaa ttggcaactc ccgcagcagg
    11941 agggcggaag cggcgtgtcg caaaatttgt ccggcaaggt ggtgttggtg accggcggtg
    12001 ccaaacgtat cggcgccgcg atcgcgcggc agttccatgc gtcggacgcg cgtctcatgt
    12061 tgcactacca cacggcgcga gccgaggcgc aggtcttgca ggccgaattg aacggtgtgc
    12121 atcccgaatc cgtggcgacg gtgcaggcgg acctcaatcg gtcggccgca ttgccggaaa
    12181 tcgttgaagc ggcagtgacg cgcttcggtc ggctcgacgt ggtggtgaac aatgcctcga
    12241 ccttttatcc cacgccggtc ggcagcatta ccgaagaggc gtggaacgac ctgatgggcg
    12301 tgaacgtgcg cgccccgctg ttcctgtcgc aagcggcggc accgtatctg cgcaagacgg
    12361 gcggttgtat cgtcaacatc gtcgacatcc acgccgaccg tccgctcaag gattacgttg
    12421 tctatagtgt ggcgaaggcc gcgctggtcg ggttgacgcg ttccctggcg cgcgaactgg
    12481 gcccggaagt gcgcgtcaat gccgtggcgc cggggccggt gctgtggccg gacagccacg
    12541 cgttcgacga acttgcgcaa cagcgcatcg tgtcgcacac cgtgctcaag cgcgtgggtg
    12601 cgcccgagga cgtggcgagg gccgtcgtct ttctggccgc ggaggcgccc tacgtgaccg
    12661 ggcaagtgct tgccgtcgac ggcgggcgca gcatcaatct ctagatccga ggaatcatga
    12721 acgccccagc cagcaagaaa atgcagaagg ccgcctacga ggcgaacaaa ctgcagaagc
    12781 gcctgcgccg ccaggttggc caggcaatcg ccgactacga catgatccgc gccggtgacc
    12841 gggtgatggt gtgcctgtcc ggcggcaagg acagctacgg cctgctcgac attctgatgc
    12901 gcctgcgcga agcggcgccg ctggatttcg aaatcgtcgc agtgaacctg gaccagcgtc
    12961 atccgggttt ccccgagcat gtgctgcccg actacctgcg tgcgctcggc gtgcccttcc
    13021 gcatcgaagt gcaggacacc tatagcatcg tcaagcgcgt tattccggaa ggaaagacga
    13081 tgtgttcgct gtgttcgcgg ctgcggcgcg gcgtgctgta tcgcgtggcg ggcgaattgg
    13141 gcgcgaccaa gatcgcgctc ggccaccatc gcgacgacat cctcggaacc tttttcctca
    13201 atctgttctt cggcggcaag ctgaaggcca tgccgcccaa gctcgtgtcc gatgacggcc
    13261 gtcatgtggt tatccggccg ctggcttacg tcaaggaagc cgatctggaa gcctatgcga
    13321 cggcgcggca gttcccgatc atcccctgcg atttgtgcgg ctcgcaggac acgctgcaac
    13381 gccagcaagt caaggcgatg ctgcgcgact gggaaaagcg cttccccggc cgggtggaaa
    13441 gcattttccg cagcctgacg gacgtgcgcc tgtcgcatct gcttgatcgg gaacgctttg
    13501 atttcacggc cgtggccgcc gacggccggc cttcggcgga cggcgaccgg gccttcgatc
    13561 cggaggattt tgcggctccg gcggcggtcc cgttgtattt gcgcgacgcc gactgagggg
    13621 cgtagagctt tacttgtcaa attgaacttg atgcggcaaa cttctgcatt acccatccgc
    13681 ccggccgaac agggtcgcgc tcccgcgacc ggaagcgacg ggcgatcgag tcccaccttt
    13741 tatgaattgc gctgaacgtc tagtcgcgtt gtttggcagt caggccgaag tcgcccgacg
    13801 gctcaaactc gatcgcgcgg tggtgaacaa ttgggtcaag tccggctacg ttccttcgcg
    13861 ctgggcgatg gagatcgagc gccttaccga aggccagatc gccgccatcg aagtcctcaa
    13921 cgaagccaac gcccgtaagc ccatccgcgt caagtcccgc ggagaggatg aaagtctttt
    13981 cggacctacg ttcgtaggaa gtgcaaccat gaatccttac gcgccccaca agcgcattca
    14041 gtccttccat ccgccgcagc gagcgctgct gggcccgggg ccctccgaga tcaatccgcg
    14101 cgtcatggcg gccatgggcc tgccggcgat cggctatctc gatcccatct tcgtggagat
    14161 gatggaggat ctgaaatcac tgctgcgtta cgtgtaccag acgaagaacg cgctgacctt
    14221 tcccatttcg gggccgggtt cggtgggtat ggagtactgc ttcgtcaata tggtggcacc
    14281 gggcgatacc gtgatcgtga tgcgcaacgg ggtgttcggc ggtcgtatga tcgaaaacgt
    14341 cgaacgctgc ggcgcgaagc cgatcgtggt cgaggacgag tggggcgagc cctgcgatcc
    14401 gaacaagctc gaggatgcgc tgaagaaaca tccggaagcg cgtgtcgtcg ccttcgtgca
    14461 tgccgagacg tccaccggcg tggcgtcgga cgccaaaacc ctgatcgacg tagcgcaccg
    14521 gcacgatgcc ctcaccatca tggatgcggt cacctcgctg ggcggctcgc cggtgctggt
    14581 cgacgagtgg cagtgcgatg cggtgtattc ggccagccag aaatgcctgt cctgcacgcc
    14641 gggtctgtcg ccggtcactt tcagcgatcg cgtggtcgac tacgtgaagt cgcgcaaaga
    14701 caagattcgc agctggttta tggacatgaa cctgctgctc ggctactggg gcaacaccac
    14761 gcggacctat caccataccg cgccgaccaa cagcctctac gccctgcatg aagcgctgct
    14821 catcctcaag gaggagggca tcgagaacgc ctgggcgcgt catcagcgcc atcacaccgc
    14881 tctcaaggcc ggcctggaat cgatggggct gcagtatctg gtcaaggaag gttatcggct
    14941 gccgcagatg aacgccgtgt acattcccga gtccctgctc gaagtcgaag ccgaagtgcg
    15001 caagcgcctg ctcaacgaat tcaatctgga aatcggcgcc ggcctggggc cgctggccgg
    15061 caagatctgg cgcatcggca tcatgggcta ctcgtgtagc tcgtcgaacg tgatgctgtg
    15121 tctgtcggcg ctgggctcgg tgttggaaga catcggctac ccgattcaag tgggcgatgc
    15181 cgaaggggcg gcgcaccagg cgtacgcgca aatgcacgcc aaagcagcga tccagaagaa
    15241 gcggcacacg gccgctgcgg cctgaacgaa ggccgtgcaa taaaaaggcg acctgcgggt
    15301 cgccttttta ttgggctatt gcggttgcgc gggctgtttg ggtggcgcga gccgggcgat
    15361 cgccgacgag cccaggacct tgccgtcctc gtccttggca agatggaagc tcatctcgac
    15421 gtaaatcttc tcttcggtct tgtgcagcga acgggtgcgc atgatgcgcc cgtggtattt
    15481 ggtgtcgccg gtttccatcg cggcattgaa gccgcgccag tgaccgtcgc gcaaattctc
    15541 cgggatgatc aaatccagcg atttgccgag cgcttcttcc ttggtaaagc cgaacagttt
    15601 ctcggcgcct tcgctccacc atcggataat gcctttccgg tcggcgtaga tgaacgcctc
    15661 gctgatgttc cggacaatgg cggcataggg tagatcgtcc tcctgcatgc ttcctccttg
    15721 tgtgcgcggt caggccagcg caccgttgag gagcagcatg atcgcgaatc ctgcggtgac
    15781 accggcagtg gcatggcttt gatggccaag acggtgggat tcgggaatga tctcatgact
    15841 gactacgaaa atcatcgccc cggcggcaaa cgccaggccc catggcaaca gtgcagccga
    15901 cacaccgatc atcgctacgc cgagcaacgc gccgatcggc tccatcagtc ccgtcgccag
    15961 cgcaaccacc gcactgcgcg cccggccgta accgagagcc agcatcgccc aggccacgat
    16021 caggccctcg gggaggttct gtatgccgat gccgagcgtg acggcagtgc cctgcgcacc
    16081 gccgccggcg taggcgacgc ccacagccaa gccctccggc acgttgtgca gcgcaatagc
    16141 gaacactacc agccagacgc gcgccatggc gtacggacgg cgcgtaggct ggtccgcatg
    16201 cgcatgcggc agaaagcggt cgattgcgag catgacaccg gcaccgagca gaatgcccag
    16261 cgcgacgacg gtgggcgaac tcaagctcag cgccggtacg gccaaagaga atgcggcggc
    16321 ggcgagcatc acgcccgcgc cgaatcccag catcgcgcta tgtgtgcgca tggacacttg
    16381 gccgacgaac aacaccggca gcgcgccggc gccggtggcc agtgcggtga aaagcgtcgc
    16441 cagcagggcc ggacgtagcg cttgcgcact tgccgctccg gacagtgcgg tctgcaacag
    16501 gaagaagatc gccaccccgc ccaaggcgag cgccagcaga acgcgcggat cgcgttgctg
    16561 taacagcttc ctggttgcaa gaatggcggt gctcatgcaa tcgctcctgt aagtgacgcg
    16621 tacaccttag tccgcgcagt gttgaaggtc caatgtattg tttatacgaa acatatagtc
    16681 aacctgtatg accagccaaa gccggtatcg ttctcgcaaa cagtccggcg tgcgccgtat
    16741 ttcttcgaaa cggatacgcc gccgcggaac ttccgcggcg gcgtatcctc cactttatgc
    16801 gtaaaggagg cactgaccca acatggcggc aacggatcga gcaggattgt tgaagcttgc
    16861 gctcatggcg gcgtgcgccg atccggcgcc cgacgcgtcc gcacaggcgc agcttcgaca
    16921 agtcgcggaa cagctttcga tgggtgcagg cgagaatctc gcgctcgcac tcagggatat
    16981 cctgctcggc cgtacgcaga tcgagcaact cgcggagccc ttgcgtcggc cggaagtcgc
    17041 attgatcgcc tatgagatgg cggtgtgcat ctgcagcgct gacagtgtgc acagcgatcc
    17101 cgaacacgct tttctggagc gccttcgcat cgcactggcg atggatgtgc gtaccgcgcg
    17161 ctcattcgag cgtacggcgg atgcagtcac aggtgtcccg ctgcaagcgg accccggccg
    17221 cgtgccgctc gccacggggc atccggtcga tgagagcttg ctggtgcgtc tggtggacga
    17281 ctacgccgtg ctcaccgctg cgctcgctgc tcgcgccgag ccccttgcgt tgcttgccac
    17341 tgttccgatc gacatgaaga tgttgtatcg catcggcaaa tattacggac aggaactcga
    17401 gcgtggacag gtgagcgcgt tcatgtgcgc tgccggcgcc gagccggttg cgcaatacgt
    17461 ggaagcgtgc ggacagttga tcattgccgg cgcgatggac cggccgcgcg cgcggtcgct
    17521 gtcgtacgat ggggtggccg cgcattgcta cgccaggacc tcggcgctgg gccgtcttgc
    17581 gcggttatgg tgcagcgaag gcgaacacaa cgacgcattg ctgcaggagc gttacacctc
    17641 atggtacgcc gaggcgcaac agggctacga ggcgcgcagg acggaagtgg ccgagcgcgc
    17701 gcggcagacg gcggtgaacg atctgctgac gctggtcaag caatgagctc cggtcctgtc
    17761 gaacggtcta ggtccacgat taagccctca gcccgatagc gacgggcagt agtgccctgc
    17821 acaatttcca cattgagaaa ccgcccgttg tggaaaagtg cgatggacac tgttcaattt
    17881 ccgctggatc caccgctgca tgaagactgg cagcgcttcg aacgtcgtgc cgctccttgg
    17941 ggcgcacgga cagcgcgtac tacgccaccg gcggctgtgt ggatcaaccg ccctgccctg
    18001 catggacgca ggcgtccccg ggcggtggcg gtgctgctca ccgtgctggg actctgcatc
    18061 gcgctttggg tatcgggctg aaacgcctca gaccgacgcg ttctgcagtt tgaagttgat
    18121 cagggtgcgc agcttggcgg gcagcaccgg cttcaccagc aggtgatagc cgtactgctt
    18181 cgcttcttcg acgcgctccg gcgtcgtgct gccggtaatg aggatggcgg gaatttccgg
    18241 gtccaggtca cgacgcaagc gctcgatcag ttgcgtaccg gtttctgcgt cggccagtcg
    18301 atagtcggca atgatcagat ccggcaaccg cccaacctgg tgaattcgat cgacaacgtc
    18361 ttcccccgtg gtggagccga tgacctcggc gccccaaccg gcaagcagta cccgcatgcc
    18421 ttccacaata gtgctttcgt cgtcgatgac gacgatgagg cgaccttgta aatctgccgc
    18481 ttgcccagcg cctcgctgct gtgcgctgcg catcaggcgc gctgcgcgcc ctcttggcag
    18541 ttcaaagcgg aaccgggtgc cgcgtcccac ttccgaagtc agctcgatgt ccgtgccgag
    18601 caggcggacg agacgccgca cgatcgacag tccgagcccc agtcccttgc gactggaacg
    18661 ttcaggattg ccgatctgat agaactcgtc gaagatgcgc tcgcgctcct cgcgcgaaac
    18721 acccatgccg gtatcccaca cttccatgct gagcttgctt tggcgcatcc ggcaggcgac
    18781 aacgatgccg cccgccgagg tgtagcggat cgcgttggcg atgaggttac gcagaatgcg
    18841 ctcgaccaga atcggatcgc tgtgcgcgac ggcgtcggat ttgcgcacat gcaggcgtaa
    18901 gcctttctcg aaggcttccg gctcgaattc cagccggagc cggtccagca cgcggctgac
    18961 gggaaaatct gtcagttcgg cacggatcac gcccgcgtcg attttggaca gatcaagcag
    19021 ttcgttgaac agcccttcca gcgcatcgac cgaagcgttg atgctgttca ccacgttcag
    19081 cacttcggga tcgcggattt tttccgacaa ggccgcggcg aacagtccca tggcgtgcag
    19141 cggctggcgc aagtcatggc ttgcagccgc aaaaaactgc gtcttcgatc gattggccgc
    19201 ctcggcttgc atacgcgcgg tatcggccaa ttgcttttgc cgcttcaact cgttgatgag
    19261 ttccacattc tcgaagcgcc ggcgtatcga ttcatgtacc agttggttga cgcgtcggcc
    19321 gaaggacagc gccgccagcg tgccgatggc gaccgggacg cccagcgcga tttcgtgcgc
    19381 gctgccgccg atgaacgcgc gcacgatgaa gggcggcgtc accagcgcgg ccagcatgta
    19441 gaacgccggc gcgaagatcg ccagcgaacc ggtcgacact gccacgatgc agaacagcag
    19501 catggccagg taggtctggc tggtggccga atccggtacg tagagcaata cgccggcgct
    19561 gccccagagc gcgccggccg tggtgatcgc gtagagatag cgttgacccc attgctccag
    19621 gtcccctccg cgagggttgg ctgcaacgaa gcgccggtag tgcagcacac gcaccatctg
    19681 gtgcacacac atggcgccga gccagccgag ccatagatag gtcggcactt cgtcgtacac
    19741 gatccacacc aggcacagtg cggcgataag cgaagccagc gtcgtggtcg gcgagtgcaa
    19801 aaagaccaga cggatctgct cgccgcggat gcgttgtgtc aggggcaact catcagcggt
    19861 atcggaacgg a
//
LOCUS       DATGOU010000121         3090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_5686876, whole genome shotgun sequence.
ACCESSION   DATGOU010000121 DATGOU010000000
VERSION     DATGOU010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5686876"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            409..1230
                     /locus_tag="VKZ48_07160"
     CDS             409..1230
                     /locus_tag="VKZ48_07160"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HLU77662.1"
                     /translation="MGIAIGPFVFSANSLVLLASIAVAFVWMRRESRRAGRDLERHFW
                     ITLLVSLVVARLGYVLTRLPHFSAAPLEALYFWQDGYLVWLGILPALLMAHALAERHK
                     YRPRHLLVPLFAGLAVWILSTWTIDNLRQATERFLPPIALNTLSNTQVEMTQYKGQPT
                     VVNLWATWCPPCRREMPVLEAAQAKHADVHFVFANQGEDAGVITRYLAAQSLDLRNVV
                     LDQRTQLSQHFAARGMPTTLFFDADGRLADMHLGELSHARLADYIDALKSAQYTK"
     gene            complement(1519..2418)
                     /locus_tag="VKZ48_07165"
     CDS             complement(1519..2418)
                     /locus_tag="VKZ48_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HLU77663.1"
                     /translation="MKKNEEQSALKCSIAVTAAIGLLGIVTGLIAGSQALVFDGMYSV
                     VDVMLTFASLAVSKLLTQEGSERFQFGYWHLEPLLIALGSSILVIACLYAAVNAVQDL
                     LGGGRVVSYGIGAAWAGVLCITCFVMTVYMGRIARRLKSGLLILDSRSWLVGAFLSLA
                     LLLSFVLAIALAGTAWEGWTPYVDAVALLCMALAILPVPVKTLLRALREVLLVAPDDL
                     DQRVRALMDELVQERGFIDYSSYVAKIGRTRMVEIHVLVGPGVSIDTATADALRREIS
                     GRLNAIWPQFWLTIDFTADRAWL"
     gene            2564..>3090
                     /locus_tag="VKZ48_07170"
     CDS             2564..>3090
                     /locus_tag="VKZ48_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007182750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HLU77664.1"
                     /translation="MWQALSMQDLGRLHDIVSTLIRYGFGDVVRRIGLANALERAGQA
                     LHWRDAKAYAHMPPPERVRRVLEELGPTFVKLGQVLATRIDLFEPEWIAEFSKLHDNA
                     PPNPYEEVRAQLIEDLGAPPEEIYARFDPEPLAAASIAQVYRARTHGGDEVVLKVRRP
                     GIRPIVEADLRWMMRL"
BASE COUNT          576 a         1020 c          951 g          543 t
ORIGIN      
        1 aaaaaccgct tgatcgtccg ctgcctctgg cctgtcaacc cccgcttttc gcagcagcca
       61 gcaggactta tcaacttgcc ggaacgccgg tatcgcgtac tttcaacggt cgtttggcgc
      121 gggctgccgc cccaccacca ccaaaagcga aagggctccc tcgtggagcc ctttggcttt
      181 tggagccggc gtggatggga acccgcgttc gggttcgact acgaaatgtc cgggggacgt
      241 ttcgttggac gccgatgcgc ctgagcgcag cggcggtcgc gagctgcatg cagcgagcga
      301 taatcggacc gcagatgcaa tgcgggccaa tcacaatccc accgccttct aacttcagcg
      361 ggacgggcgc cccctctcat caattcggct accgcctaaa ctcgccccat gggcatcgcc
      421 atcggacctt ttgttttttc cgcaaacagc ctggtgctgc tcgcgtcgat cgccgtcgca
      481 ttcgtctgga tgcgccgcga aagccgacgc gcgggccgtg atctggaacg tcacttctgg
      541 atcacgctgc tcgtcagtct cgtcgtcgcc cgcctcggct acgtcctgac ccgcctgccg
      601 cacttcagcg ccgcgccgct cgaggcgctc tatttctggc aggacggtta cctcgtctgg
      661 ctcggcatcc tgccggcact gctgatggcg catgctctcg cagaacggca caagtatcgc
      721 ccgcgacatc tgctggtgcc gttgttcgcg gggctcgccg tctggatcct gtccacctgg
      781 acgatcgaca atttgcggca ggccaccgaa cgcttcctgc cgccgatcgc gctcaacacg
      841 ttgagcaata cgcaggtgga gatgacgcaa tacaaggggc agcccacggt cgtgaatctg
      901 tgggcgacct ggtgcccgcc ctgtcgacgc gagatgcccg tcctcgaggc cgctcaggca
      961 aagcacgccg acgttcattt cgtattcgcg aaccagggcg aggacgcggg tgtcatcacg
     1021 cgttatctcg ccgcgcagtc gctcgatctc cgcaatgtcg tcctcgacca gcgcacacag
     1081 ttgtcgcagc acttcgccgc gcgcggcatg cccacgacct tgttcttcga cgccgacggc
     1141 cggcttgcgg acatgcatct gggtgaattg agtcatgcgc gtcttgcgga ttacatcgac
     1201 gcgctcaaaa gcgctcaata cactaagtag aaaattgcga cgatccctgg aggatgagcg
     1261 gcgatgaaga ttgccgaacg cccgaccgac atgctcctgc ggcagatgta cgccctgcgg
     1321 aagcacgctc aattccgtca tccggagacg ggtcgcagag gacacgggca tgaatcatcg
     1381 ttccgggccg cattcgtgac cggcatgctg gaattgcgaa ccgagttgcg gaagcgcggg
     1441 atccattgga cccgaacgag aaaatgacct agccgcgggc ttgcgcgctg cccggtattc
     1501 ggtacgattc gctcgccgtc acaaccacgc ccgatcggcc gtgaaatcga tggtgagcca
     1561 gaactgaggc cagatcgcat tcagacgccc cgagatttcg cggcgaagcg cgtcggccgt
     1621 tgcggtatcg atcgaaacgc cgggcccgac gagcacgtgg atttcgacca tccgcgtacg
     1681 gccgattttc gcgacgtaac tggaatagtc gataaagccg cgttcctgca ccagttcatc
     1741 catgagcgcg cgcacccgct ggtccagatc gtcgggcgcc acgagcagca cttcgcgcag
     1801 cgcgcgcagc agcgtcttca ccggtacggg caatatggcg agcgccatgc agagcagcgc
     1861 gacggcatcg acgtagggcg tccatccttc ccatgcggtt cccgcaagcg cgatggccag
     1921 tacgaaggaa agcagcagcg cgagactgag gaatgcaccg accagccaac tgcgcgaatc
     1981 gaggatgagc agtcccgatt tcagccggcg tgcgatacgg cccatgtaca cggtcatgac
     2041 gaaacacgtg atgcagagaa cacccgccca cgcggcgccg atgccgtagg agacgacacg
     2101 cccgccgccc agcaggtcct gaaccgcatt gaccgcagcg tagaggcagg cgatcaccag
     2161 aatggaactg cccagtgcga tgagcagcgg ttccagatgc caatagccga actggaagcg
     2221 ttcgctgcct tcctgggtga gaagcttcga gacggccagt gaggcgaagg tcagcatcac
     2281 atcgacgacg ctgtacatgc cgtcgaacac cagcgcctgc gagccggcga tcaagcccgt
     2341 gacgatgccc agcagcccga tggctgccgt gaccgcgatg gaacacttga gtgcgctttg
     2401 ctcttcgttc ttcttcatgc ctcggctgcc cctgccaaac ggcgatcggt acggcgatca
     2461 tacgtgcgga ccggacggcg gtctgctccc tgccgtcgcg caccgcagcg cgagaggcac
     2521 cgccttcggt atgatgcggc atctcttccc cgccgaaagc gccatgtggc aagccctttc
     2581 gatgcaggac ctcggccgtc tgcatgacat cgtctccacg ctgatccgct acggtttcgg
     2641 cgacgtggtg cgccgcatcg ggttggcgaa cgccctggag cgcgccggac aggcgttgca
     2701 ctggcgcgat gcaaaggcct atgcgcacat gccgccgccc gagcgcgtgc ggcgcgtgct
     2761 ggaggaactg gggccgacct tcgtcaaact cggtcaggtg ctcgccaccc ggatcgacct
     2821 gttcgagccg gagtggatcg ccgaattcag caagctgcac gacaacgcgc cgccgaatcc
     2881 ctatgaggaa gtgcgggcgc aactgatcga ggacctgggc gcgccgcccg aagagatcta
     2941 cgcccgcttc gatcccgagc cgctggcggc cgcttccatc gcgcaggtct atcgcgcccg
     3001 cacgcacggc ggcgacgagg tcgtgctcaa ggtgcggcgg ccgggcattc gtcccatcgt
     3061 cgaggcggat ctgcgctgga tgatgcgcct
//
LOCUS       DATGOU010000122         7577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_5698217, whole genome shotgun sequence.
ACCESSION   DATGOU010000122 DATGOU010000000
VERSION     DATGOU010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7577
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5698217"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VKZ48_07175"
     CDS             complement(<1..249)
                     /locus_tag="VKZ48_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HLU77665.1"
                     /translation="MDKLAIQGGVPLEGEVRISGAKNAALPLMCAALLTKEPLRLRNV
                     PHLRDVSTMLRLLGEMGVDVTMDECQGVILNAASLREPV"
     gene            complement(242..481)
                     /locus_tag="VKZ48_07180"
     CDS             complement(242..481)
                     /locus_tag="VKZ48_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011517725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HLU77666.1"
                     /translation="MVRPEDVQRYIEEGMPCDLVRVIGDGHHFEAIIVSGEFRGKNKV
                     QQHQVVYKALGDRMRAEIHALSMQTYTPEDWAARG"
     gene            complement(493..1269)
                     /locus_tag="VKZ48_07185"
     CDS             complement(493..1269)
                     /locus_tag="VKZ48_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLU77667.1"
                     /translation="MKQESEIRGGFSTLLYKELLRFWKVGFQTIAAPILTAMLYLLIF
                     GHVLEDHVEVYENVGYTSFLVPGLVMMSMLQNAFANSSSSLIQSKVTGNVVFVLLPPL
                     SYLEFYGGYLIAAMIRGLAVGLGVLLVALPFVSLRMEAPVWVILFAIAGTGILGTLGV
                     IAGIWADKFDQLAAFQNFIILPLTFLSGVFYSIYSLPPFWQDLSHLNPFFYMIDGFRY
                     GFFGVSDVDPRLSFVVVVLFNALLTATTLALLKRGYKLRH"
     gene            complement(1266..2171)
                     /locus_tag="VKZ48_07190"
     CDS             complement(1266..2171)
                     /locus_tag="VKZ48_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLU77668.1"
                     /translation="MTPAIDINGLTKRYRSLQALGGVDLHIRQGEFFGLLGPNGAGKT
                     TLISVLAGLVRPTTGTARVMGHDVVREYRAARRMLGVVPQELVFDPFFSVRETLRIQS
                     GYYGLKRNDDWIDEVMHHLNLSDKADANMRALSGGMKRRVLVAQALVHRPPVIVLDEP
                     TAGVDVELRRGLWMFIRQLNRAGHTIVLTTHYLEEAETLCNRVALLKLGRIVALDTPA
                     SIIRRFQAIYLRVRVEGALPPALEGKASVDEVGRQLFKLDDYGQVETTLAALRAAGCR
                     LLEVEVTQPDLEEAFVNLVRGVGQA"
     gene            complement(2197..2490)
                     /locus_tag="VKZ48_07195"
     CDS             complement(2197..2490)
                     /locus_tag="VKZ48_07195"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HLU77669.1"
                     /translation="MRAVLQDDRLILEGEINSTTVAGLLDGALAHVREGAETVDFSGV
                     SDVDSAAVALALACIREAQARNHTLRFANLPPKFNDLARLYNVSELFLDAAAV"
     gene            complement(2487..3083)
                     /locus_tag="VKZ48_07200"
     CDS             complement(2487..3083)
                     /locus_tag="VKZ48_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLU77670.1"
                     /translation="MLLLAFGLLPAAQAETAPDELVKSVTAEVIEIVKNDEDIKKGDP
                     RKAAQLVQEKVLHHFNFTRMTALAMGVSWRQATPEQRRSLVDQFRTLLVRTYSTALTS
                     YRDQTIDYAPLRMQPGDKDVTVRSLVRQPGAQPVTIDYRMEKTSDNWKVYDVAVGGVS
                     LVTTYRDTFNTEVRNSGIDGLLKTLMEKNQQLANQSNG"
     gene            complement(3114..3980)
                     /locus_tag="VKZ48_07205"
     CDS             complement(3114..3980)
                     /locus_tag="VKZ48_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HLU77671.1"
                     /translation="MKNTLMQSGARIAAIALALAVLSGCATTHADPRDPLEPFNRAMF
                     EFNDTLDKAVLKPVAQGYKAVMPSPVRTGVTNFFSNLGDPWIALNQLLQGKVEEGMTD
                     FMRFVVNTTFGLCGIFDVASEGGMEKHDEDFGQTLGVWGLEPGPYLVVPFLGPSSVRD
                     GAGWVPDSYGYLPWRGPKLLDAGDEVALRNALTATDTVQTRANLLDATNVMEEAALDR
                     YGFVRNAYLQRRQSQVYDGNPPVESFDDYEDYDLDYDLDKPAAAPGGDAGGPAPAAPG
                     RDDSADAPAKAR"
     gene            complement(4015..4497)
                     /gene="mlaD"
                     /locus_tag="VKZ48_07210"
     CDS             complement(4015..4497)
                     /gene="mlaD"
                     /locus_tag="VKZ48_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HLU77672.1"
                     /translation="MSRKAIDVWVGAFVLIGIAALFFLALQVASSSDAFNGGAGYSVQ
                     ARFENIGGLKPRAPVRSAGVLVGRVTDINFDNQTFEAVVTMNLDKRFAFPKDTAAKVL
                     TSGLLGEQYVGLTPGGDLADLGDGDTIRITQSAIVLENLIGQFLYSKAAGDDQNAPAR
                     "
     gene            complement(4507..5295)
                     /gene="mlaE"
                     /locus_tag="VKZ48_07215"
     CDS             complement(4507..5295)
                     /gene="mlaE"
                     /locus_tag="VKZ48_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HLU77673.1"
                     /translation="MNALLNFVERLGAYVNHRIARLGFAARFLWVMLRTSGTSLMRLH
                     LTIREIYFAGVLSLIIVSVSALFVGMVLALQGYDTLVRFGAAEAVGTMVALSLVRELG
                     PVIAALLFASRAGSAITAEIGLMKATEQLAAMEMMAVDPIARVVGPRFWGGVVSMPFL
                     AAIFSAIGVLGGYLIAVVLIGVDFGSFWSQMQAAVDWRSDVVNGLIKSFVFGIAVSWI
                     AVFEGYDAMPTAEGVSRATTRTVVISALAILMLDFILTAFMFRG"
     gene            complement(5292..6098)
                     /locus_tag="VKZ48_07220"
     CDS             complement(5292..6098)
                     /locus_tag="VKZ48_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLU77674.1"
                     /translation="MELPPIVEFDDVSFSYGPRAVLNGMSLSVHKGQVVAIMGASGSG
                     KTTVLRLMGGALRASKGEVRAFGQTLNRMRTRDLYEMRRDMGMLFQFGALFTDLSVFD
                     NVAFPIRERTDLPERLVRDLVLMKLETVGLRGAAHLRPQQLSGGMARRVALARAIALD
                     PQLILYDEPFAGLDPISLAITGKLIRDLNTALGAASVIVTHDVQESLQIVDHIYFVSE
                     GRIVADGTPDEVRRSDIPFVEQFVQAHTDGPVPFHYPAQQPYREELLGQV"
     gene            6344..>7577
                     /locus_tag="VKZ48_07225"
     CDS             6344..>7577
                     /locus_tag="VKZ48_07225"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLU77675.1"
                     /translation="MTNNTKPGLLIVDDDPLIADTLAFALGKDFEVILSDSRVHAISL
                     LRQMDEPPHLALVDLGLPPTPHRPDEGFQLIADLLAHSPSIKILVLSGQNDAANARHA
                     RTLGAAEFVAKPCDPEVLKKTLLHALEFRSAEVSSSAAVEAGMLIGRSAAMQKLRSQI
                     SQYADSPFPALIEGESGSGKEMVANALHRLSSRKDKPCLALNCAAIAPTLVEPTLFGY
                     VKGAFTGAQTNKSGYFEDAADGTLFLDEIGELPMELQAKLLRVLENGEYQRVGETQRR
                     YSRARVIAATNRDLRQEIRKGNFRADLYHRLSVFTIGVPPLREMDDDKLMLLEHFSAF
                     YAERAQLPAFMLDESAMKLWRHYHFPGNVRELRNIVIRLTTKYAGQRLSAVELEAELD
                     VSTPDDYGAPELSLPNDPE"
BASE COUNT         1460 a         2479 c         2342 g         1296 t
ORIGIN      
        1 gacgggttcg cgcagggacg cggcgttgag aatcacgccc tggcactcgt ccatggtcac
       61 atccacgccc atctcgccca gaaggcgcag catggtggac acgtcgcgca aatggggaac
      121 attgcgcagt cgcagcggct ccttggtgag cagcgccgcg cacatcagcg gcagggcggc
      181 gttctttgcg ccggagatgc ggacttcgcc ttcgagcggc acgccgccct ggatcgcaag
      241 tttatccacg cgccgcccag tcttccggcg tgtacgtctg catcgacagg gcgtggatct
      301 cggcgcgcat gcggtcgccc agcgccttgt aaacgacctg atgctgctgc accttgttct
      361 tgccgcggaa ctcgccgctc acgatgatcg cttcgaaatg atgaccgtcg ccgatcaccc
      421 gcaccagatc gcagggcatg ccttcttcga tataccgctg cacatcctcg ggacgaacca
      481 taacttcctc acttaatgtc gtaatttgta gccacgtttc aggagcgcta gtgtagtcgc
      541 tgtcaacagg gcattgaaca gcacgaccac cacgaagctc agacgcggat cgacgtcgga
      601 cacgccaaag aaaccatagc ggaatccgtc gatcatgtag aaaaagggat tcaggtggga
      661 caaatcctgc caaaacggtg gcaacgagta gatcgagtag aaaacgccgg acaggaaggt
      721 gagcggcagg atgatgaagt tctggaacgc cgcgagctga tcgaacttgt ccgcccagat
      781 gccagcgatc acgcccagcg tgcccaggat gccggtgccg gcaatcgcga acaggatcac
      841 ccacacgggt gcttccatgc gcaaggacac gaagggcagc gcgacgagca gcacgcccag
      901 ccccaccgcc aggccgcgga tcatcgcggc aatcagatag ccgccgtaga actccagata
      961 ggacagcggc ggcagcagca cgaacaccac gttgcccgtc accttggact ggataaggct
     1021 cgacgaactg ttggcgaagg cgttctgcag catcgacatc atcaccagac cgggcacgag
     1081 gaaggaggtg tagccgacgt tctcgtacac ctcgacgtga tcctccagca catggccgaa
     1141 gatcagcaga tagagcatgg ccgtgaggat cggtgcggcg atggtctgga agcccacttt
     1201 ccagaagcgc aacaattcct tgtacagcag ggtgctgaag ccgccgcgaa tctcgctctc
     1261 ctgcttcatg cctgccccac gccgcgtacc aggttcacga aggcttcctc gagatcgggc
     1321 tgcgtcactt cgacttcgag caggcggcaa ccggccgcgc gcaacgcggc cagcgtcgtc
     1381 tccacctgcc cgtaatcgtc gagcttgaac aactgacggc ccacttcgtc cacgctggcc
     1441 ttgccttcca gcgcgggcgg cagcgcaccc tcgacgcgca cgcgcaaata aatggcctgg
     1501 aagcggcgga tgatgctggc cggggtatcg agcgcaacga tcctgcccaa cttcagcagc
     1561 gcaacgcgat tgcagagcgt ctcggcctct tccagataat gggtggtgag cacgatggtg
     1621 tgaccggcgc gattcaactg gcggatgaac atccacagcc cccgtcgcaa ctccacgtcc
     1681 acacccgcag tcggctcgtc gagcacgatc accggcgggc gatgcaccag cgcctgcgcc
     1741 accagcacgc ggcgcttcat gccgccggag agcgcgcgca tgttggcatc cgccttgtcg
     1801 ctgagattga ggtgatgcat gacttcgtcg atccaatcgt cgttgcgctt aagcccgtag
     1861 tagccggact ggatgcgcag ggtttcgcgc acgctgaaga agggatcgaa caccagttcc
     1921 tgcggcacca cgccgagcat gcgccgcgcc gcccggtatt cgcgcaccac gtcatgcccc
     1981 atgacgcgcg cagttccggt ggtggggcgg accagcccgg cgagcacgct gatcaacgtg
     2041 gtcttgccgg cgccgttcgg gccgagcaga ccgaagaact cgccctgccg gatgtgcaga
     2101 tcgacgcccc cgagcgcctg cagcgaccgg tagcgtttgg tcaagccatt gatatcgatt
     2161 gccggggtca tcgcgctccg cactgcattc ggggcgctaa accgcggcgg cgtcgaggaa
     2221 gagttccgag acgttgtaga gccgtgccag atcgttgaac ttggggggca gattggcgaa
     2281 acgcaaggta tggttgcgtg cctgcgcttc gcggatacag gcgagtgcaa gcgccactgc
     2341 cgcagaatcc acgtcgctta ccccactgaa gtcgacggtc tcggcgcctt cgcgcacgtg
     2401 cgccagcgcg ccgtcgagca ggcctgcaac ggtggtgctg ttgatttcgc cttccagaat
     2461 gaggcggtcg tcttggagta ccgccctcat ccgttgcttt ggttggccag ttgctggttc
     2521 ttttccatca gcgtcttgag cagcccgtcg atgcccgaat tgcgcacctc ggtgttgaag
     2581 gtgtcgcggt aggtcgtcac gaggctcacg ccgccgaccg ccacgtcgta caccttccag
     2641 ttgtcgctgg tcttttccat tcggtaatcg atggtgaccg gctgcgcgcc cggctgacgc
     2701 accagcgagc gtaccgtgac gtccttatcg cccggctgca tgcgcagcgg cgcgtaatcg
     2761 atggtctggt cgcggtagga ggtcaacgcg gtggaatagg tacgcaccag caaggtgcgg
     2821 aactgatcca ccagcgaacg gcgctgttcc ggggtagcct gccgccagct gacgcccatc
     2881 gccagggcgg tcatgcgggt gaagttgaag tgatgcagca ccttttcctg caccagttgg
     2941 gcagccttcc gcggatcgcc cttcttgatg tcctcgtcat tcttgacgat ttcgatgacc
     3001 tcggcggtca ccgacttcac cagttcgtcg ggcgccgtct ccgcctgcgc ggcaggcagc
     3061 aggccgaagg caagcagaag catcaacgca atacgcatag tgttctcgca gtgtcagcgc
     3121 gccttggcgg gcgcgtcggc agaatcatcg cgacccggcg cagccggcgc cggaccgccc
     3181 gcatcgccgc ccggggcggc ggcaggtttg tcgaggtcat agtcgaggtc gtagtcttcg
     3241 tagtcgtcga aggactcgac cggcgggttg ccatcgtaca cctggctctg gcgccgctgc
     3301 agataggcgt tgcgcacaaa gccgtagcga tcgagggccg cctcctccat cacattggtc
     3361 gcgtcgagta agttggcccg cgtctggacc gtgtccgtgg cagtcagtgc gttgcgtaat
     3421 gcaacttcgt cgcctgcgtc gagcagcttg ggaccgcgcc agggcagata gccgtagctg
     3481 tcgggcaccc agccggcgcc gtcgcgcacg ctgctcggac cgaggaaggg caccaccaga
     3541 taaggaccgg gctccagtcc ccagacaccg agcgtctgac cgaagtcctc gtcatgcttt
     3601 tccatgccgc cttcgctggc cacatcgaaa atgccgcaca ggccgaaggt cgtattgacc
     3661 acgaagcgca tgaagtcggt catgccctct tcgaccttgc cctgcagcaa ctggttgagg
     3721 gcaatccagg ggtcacccag gttggagaag aagttggtca cgccggtgcg gacagggcta
     3781 ggcatcaccg ccttatagcc ttgcgccacc ggcttgagca cggccttgtc cagggtgtcg
     3841 ttgaactcga acatggcgcg attgaacggc tcgagcggat cgcgcggatc ggcatgagtc
     3901 gtcgcacagc cgctcagcac cgcgagcgcc aacgcgatag ctgcgatgcg cgcgccggac
     3961 tgcataaggg tgtttttcat cttgtgttct tcgttcgcca tggccgacca gcaatcaacg
     4021 tgctggcgcg ttctggtcgt cgccggccgc cttactgtac aggaactgtc cgatcagatt
     4081 ttccagcacg atggccgact gggtgatgcg gatcgtgtcg ccgtccccga gatcggcgag
     4141 atcgccgccc ggcgtcaggc cgacgtactg ttcgcccagg agacccgaag tcagcacctt
     4201 cgcggccgtg tccttgggga aggcgaagcg cttgtccaga ttcatcgtca ccaccgcctc
     4261 gaaggtctgg ttgtcgaaat tgatgtcggt cacccgcccg accagcacgc cggcgctccg
     4321 cacgggcgca cggggcttca gaccgccgat gttttcgaag cgcgcctgca cgctgtaacc
     4381 ggcgccgccg ttgaacgcat cgctgctgct cgccacctga agcgccagga agaacagcgc
     4441 cgcgatgcct atcagtacga atgcgccgac ccacacgtct attgccttac ggctcatcat
     4501 ttcttcttac ccccggaaca tgaacgcggt caggatgaaa tccagcatca ggatcgccag
     4561 cgccgagatc accaccgttc gagtcgtcgc acgcgacacg ccttcggcgg tcggcatggc
     4621 atcgtagcct tcgaacactg cgatccacga aaccgcgatg ccgaagacga aagatttgat
     4681 gaggccgttc accacgtcgc tgcgccagtc cacggcggcc tgcatctgcg accagaacga
     4741 gccgaaatcc acgccgatca gcaccaccgc gatcagatag ccgcccagca cgccgatcgc
     4801 cgagaagatc gccgccagga agggcatgga gaccacgccg ccccagaatc gcggcccgac
     4861 cacgcgggcg atagggtcga ccgccatcat ttccatggcg gccagttgct cggtcgcctt
     4921 catcaggccg atttcggccg tgatggccga tccggcgcgg ctggcgaaga gcagcgcagc
     4981 gatcaccgga ccgagttcgc gcaccagcga cagcgccacc atggtaccga ccgcttcggc
     5041 agcgccgaag cgcaccagcg tatcgtagcc ctgcagcgca aggaccattc cgacgaacag
     5101 cgcggaaacg gacacgataa tcagcgatag gacgccggcg aaataaatct cgcggatagt
     5161 caggtgcagg cgcatcaggc tggtgcccga cgtcctgagc atcacccaca ggaagcgcgc
     5221 ggcgaacccc aggcgcgcga tgcggtgatt gacgtatgcg ccgaggcgct cgacgaaatt
     5281 cagcagggcg ttcatacctg accgagcagt tcctcgcgat agggttgctg cgcggggtag
     5341 tggaagggga ccgggccgtc ggtatgcgcc tgcacgaact gctccacaaa gggtatatcc
     5401 gagcggcgca cctcgtccgg cgtgccgtcc gcgacgatgc gcccttccga aacgaagtag
     5461 atgtgatcca cgatctgcag cgattcctgc acgtcgtggg tgacgatgac cgacgccgcg
     5521 cccagcgcgg tattcaggtc gcggatgagc ttgccggtaa tcgcgagcga aatcgggtcc
     5581 agtccggcga agggctcgtc ataaagaatc agttgcggat cgagcgcgat ggcccgcgcc
     5641 agcgccacgc gccgcgccat cccgccggac agttgctggg ggcgcagatg cgccgcgccg
     5701 cgcaatccaa ccgtttcgag cttcattagc accagatcgc gcaccagccg ctccggcaga
     5761 tcggtgcgct cgcggatggg gaaggccacg ttgtcgaaca cgctcaggtc ggtgaacagc
     5821 gcgccgaact ggaacagcat gcccatgtcg cgccgcattt catacagatc ccgcgtacgc
     5881 atgcggttga gcgtctggcc gaaggcgcgc acttcgcctt tggacgcccg caaggcgccg
     5941 cccatcagcc gcagcacggt cgtcttgccg gaaccgctcg cgcccatgat ggccacgacc
     6001 tgccccttgt gcaccgatag cgacatgccg ttcaggacgg cgcgcgggcc gtacgagaac
     6061 gaaacgtcgt cgaactctac gatcggaggc aattccatgc gggacggtgt gaaaaacgtc
     6121 gcattgtaat cgaagggccc aacccgctct actcggccgg gcggtctagc ggtttttagc
     6181 acgccggcat cccgcccccg ctgaccgaag tcaagcgcaa caccgtgcgc cgaatggtct
     6241 tgcgccgtca tgtgccgtat tacgcaggct atgccgccgt gctatgcgac cattgccttc
     6301 tcctagaaga tcgcctcgcc gcaagccgtc gaggcgcccg cacatgacca acaacacaaa
     6361 accgggactt ctcattgttg acgatgatcc gctcatcgcg gatacgctcg ccttcgcgct
     6421 gggcaaggat ttcgaagtca tcctgagcga ttcgcgcgtg cacgccatca gtctgctgcg
     6481 tcagatggac gagccgccgc atctggccct ggtggacctg ggcctgccgc ccaccccgca
     6541 ccggccggac gaaggcttcc agctcattgc cgatctgctc gcgcattcgc cgagcatcaa
     6601 gattctggtg ctttccggac agaacgacgc ggccaacgcc cgtcatgccc gcacgctcgg
     6661 cgcggcggag ttcgtggcca agccttgcga tccggaagtt ttgaagaaga cgctgctgca
     6721 cgcgctcgag ttccgcagcg cggaggtgtc gagcagcgcc gcggtcgaag cgggcatgct
     6781 catcggccgc agcgcggcca tgcagaagct gcgcagccag atcagccaat acgccgattc
     6841 gcccttcccc gcgttgatcg aaggcgaatc cgggagcggc aaggagatgg tggccaatgc
     6901 gctgcatcgc ctgtccagcc gcaaggacaa accctgcctc gcgctcaact gcgcggccat
     6961 cgcgccgacg ctggtcgagc ccacgctgtt cggttatgta aagggcgcct tcaccggcgc
     7021 gcagacgaac aaatccggct atttcgagga cgccgccgac ggcacactgt tcctcgacga
     7081 aatcggcgaa ctgccgatgg aactgcaggc caagctgctg cgcgtcctgg agaacggcga
     7141 ataccagcgc gtcggcgaaa cgcagcgacg ctacagccgc gcgcgcgtga tcgccgcgac
     7201 caaccgcgat ctgcgccagg agatcaggaa gggcaatttc cgcgcggatc tttatcaccg
     7261 cctgtccgtg ttcaccatcg gcgtgccgcc gctgcgcgaa atggacgacg acaagctgat
     7321 gctgctcgaa cacttcagcg ccttctacgc cgagcgcgcg caactgcccg cgttcatgct
     7381 cgacgagagc gcgatgaagc tctggcggca ctatcacttc ccgggcaatg tgcgcgaact
     7441 gcgcaacatc gtcattcgcc tcacgaccaa gtacgccgga cagcggctgt ccgcggtcga
     7501 actggaggcg gaactggacg tcagcacgcc cgacgactac ggcgcaccgg aactgtcgtt
     7561 gccgaacgat ccggaag
//
LOCUS       DATGOU010000123         4713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_2174693, whole genome shotgun sequence.
ACCESSION   DATGOU010000123 DATGOU010000000
VERSION     DATGOU010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2174693"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1488
                     /locus_tag="VKZ48_07230"
     CDS             <1..1488
                     /locus_tag="VKZ48_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HLU77676.1"
                     /translation="EPFRNMEYRSLARNGSQVWQLVNGVPIIDPEGQLTGYRGTALDI
                     TERKIAEARITELATRDPLTGLPNRLLLNDRLAQGITAAHRSGEMLAVMFLDLDHFKH
                     VNDSLGHDVGDQLLKDVAKRIGNVLRKGDTLSRLGGDEFVVVLTGLRSADDASQVAQK
                     IIDSLCQDYEIAQHIVTTACSIGISVYPTDGVEPGVLMRHADTAMYVAKSSGRKNYQF
                     FSAEMNARANERLNIERSLRGAIEREELQVYYQPRVDLSSGALTGVEALLRWHHPEHG
                     LILARDFLSVIEESGLIHSVGEWVLESACAQAQAWQARYNETFSISVNVSGRQFNRGL
                     PARISAALESSGLDPRVLEIEITELSLNHSIEATRTVLDDLRKLGVRVVVDDFGTGVS
                     SMSHLRRFNVDGIKIDRSFVRGMMSSADDRIVVKAMIEMARSLRINTIAEGVESEEQL
                     ELLRTMGCEEYAGHLLGEPATAAAFEQRWLQPENVISLAPRRMAD"
     gene            complement(1630..2901)
                     /locus_tag="VKZ48_07235"
     CDS             complement(1630..2901)
                     /locus_tag="VKZ48_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLU77677.1"
                     /translation="MRFETLAIHAGYSPEPTTKAVAVPIYQTTSYAFDDTQHGADLFD
                     LKVQGNIYTRIMNPTNDVLEQRVTALEGGVGALALASGQAAVTYSVMTIAEAGDNIIA
                     ISTLYGGTYNLFAHTLPQYGIEVRFVDATDPAAVAARIDARTKAVFCESIGNPLGNVV
                     DFAAFADVAHAHGVPLIVDNTVPTPYLTRPFEHGADIVVHSLTKYMGGHGTSIGGIIV
                     DSGKFPWAEHKARFPRLNEPEVSYHGVVYTEALGPAAYIGRVRTVPLRNMGAAMSPFN
                     SFLIMQGIETLSLRMDRHCENALKVAEYLQSHPKVAWVNYAGLPDHASRPLVDKYFGG
                     KAAGLLTFGVKGGLAAGTRVQDAFKLITRLVNIGDAKTLACHPASTTHRQLSPEELKK
                     AGVSEDMMRLSIGIEHIDDIIADLDQALAQA"
     gene            complement(2976..3440)
                     /locus_tag="VKZ48_07240"
     CDS             complement(2976..3440)
                     /locus_tag="VKZ48_07240"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLU77678.1"
                     /translation="MAALRLTVIAHGSDAYREAVELRHEVLRKPLGLNFTPQQLAAEA
                     DALHYAAYAGERLVATAYLLPLDPARAHLRQMAVSPGMRASGVGGALLAYLEDDARRR
                     GYREIVADARVSALGFYLKQGYTAHDEVYDHVGLPHRRIHKALIDPKAESTR"
     gene            complement(3447..4625)
                     /locus_tag="VKZ48_07245"
     CDS             complement(3447..4625)
                     /locus_tag="VKZ48_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HLU77679.1"
                     /translation="MLIDFFYRLRAAKVPVSLKEFLTLLEALERGVIGPSVDGFYYLS
                     RAALVKDETHFDKFDRAFGEYFEGVESIPLEEALIPEEWLRRLKELKLSPEEMAAIEA
                     MGGLDKLMETLKKRLEEQKERHQGGNKWIGTAGRSPFGAYGYNPEGIRIGQHESRHRR
                     AVKVWDEREFRNFDSEVELGTRNIKVALRRLRQFARSGAAEELDLESTISRTAKNAGW
                     LDLQMRPERHNAVKVLLLLDVGGSMDDHIQLCEELFSACKSEFKHLEHYYFHNCLYES
                     VWKDNAYRRKDRIGTMDLLHKYGSDYKLIFVGDATMGPYEITEPGGSVEHWNEEPGIV
                     WMRRMLDVYPKAVWLNPESEEMWPYRYSIGLMRQIMDGRMYPLTLDGLDRAMRYLSSS
                     "
BASE COUNT          824 a         1465 c         1542 g          882 t
ORIGIN      
        1 gagcccttcc gcaacatgga ataccgctcg ctggcgcgta acggcagcca ggtctggcaa
       61 ctggtgaacg gcgtgcccat catcgacccg gaaggccagt tgacggggta tcgcggtacc
      121 gcgctcgaca tcaccgaacg caagatcgcc gaggcgcgca tcaccgagct ggcaacgcgc
      181 gatccgctca ccgggctgcc gaaccgcctg ctgctcaacg accggctggc gcagggcatc
      241 accgccgcgc atcgcagcgg cgagatgctc gcggtgatgt tcctcgatct cgaccacttc
      301 aagcacgtca acgactcgct cggccacgac gtcggcgacc agttgctcaa ggacgtggcc
      361 aagcgcatcg gcaacgtcct gcgcaagggc gacacgctgt cgcgcctggg cggtgacgaa
      421 ttcgtggtgg tgctgaccgg cctgaggagc gccgacgacg ccagccaggt agcgcagaag
      481 atcatcgatt cgttgtgcca ggattacgaa atcgcccagc acatcgtcac caccgcctgc
      541 agtatcggca tctccgtgta tccgaccgac ggcgtcgagc ccggcgtgct gatgcgccat
      601 gccgacacgg ccatgtacgt ggcgaaatcg agcggacgga agaattatca gttcttctcc
      661 gccgaaatga acgcacgcgc caacgaacgg ctcaacatcg agcgcagcct gcgcggcgcg
      721 atcgagcggg aggaactgca ggtctactat cagccgcgcg tcgatctatc gagcggcgcg
      781 ctgacgggcg tcgaagcgct gctgcgctgg catcatcccg agcacggcct catcctcgcg
      841 cgcgacttcc tgtcggtgat cgaggagtcc ggattgatcc attccgtggg cgaatgggtg
      901 ctggaaagcg cctgcgccca ggcgcaggcc tggcaggcgc gctacaacga aaccttttcc
      961 atttccgtca acgtgtcggg ccggcagttc aaccgcggcc tgcctgcacg catcagtgcg
     1021 gcgctggaat cgagcgggct cgatccgcgc gtgctggaaa tcgaaatcac cgaactgtcg
     1081 ctcaaccaca gcatcgaggc cacgcgcacg gtgctcgacg atctgcgcaa gctcggtgta
     1141 cgcgtggtgg tggacgactt cggcaccggc gtgtcctcca tgagccatct gcgccgcttc
     1201 aacgtggacg gcatcaaaat cgaccgctcc ttcgtgcgcg gcatgatgag cagcgccgac
     1261 gatcgcatcg tggtcaaggc gatgatcgaa atggcgcgaa gcctgcgtat aaataccatc
     1321 gccgaaggcg tggagagcga ggaacaactg gaactcctgc gcaccatggg ttgcgaggaa
     1381 tatgccggtc atctgctcgg cgaacccgcc acggcagcgg cgttcgagca gcgctggctg
     1441 cagccggaga acgtcatttc gcttgcgccg cgacggatgg cggactagcg atctgcacta
     1501 ccggcgtcac cgtcgtgccc cagcgccgct tggtctcgtc gcctgcttct gcgcgatctc
     1561 tatctgaaca atgagcgagg ctctggccga atgcgcgatg cccgcttgtg ccggcatcgc
     1621 gcatgagctt caggcttgag ccagcgcctg gtccagatcc gcaatgatgt cgtcgatatg
     1681 ctcgatgccg atcgacagcc gcatcatgtc ctcgctgacg ccggctttct tcaactcctc
     1741 ggggctcagt tgacggtgcg tggtggaggc cgggtggcag gcgagggtct tggcgtcgcc
     1801 gatgttcacc agccgcgtga tcagcttgaa ggcgtcctgc acgcgcgtgc cggctgcgag
     1861 gccgcccttg acgccgaagg tgagcaggcc cgcggccttg ccgccgaaat acttgtccac
     1921 caggggccgg ctggcgtgat cgggcaggcc ggcgtagttg acccaagcga ccttggggtg
     1981 gctctgcaga tactcggcga ccttgagcgc gttctcgcag tgacggtcca tgcgcaggct
     2041 cagcgtttcg atgccctgca tgatgaggaa gctgttgaag ggcgacatgg cggcgcccat
     2101 gttacgcagc ggcacggtgc ggacgcggcc gatgtacgcc gccgggccga gggcttcggt
     2161 gtacaccacg ccgtggtagc tcacctccgg ttcattgagg cgggggaagc gcgccttgtg
     2221 ttcggcccag ggaaacttgc cggaatcgac gatgatgccg ccgatgctcg tgccgtgccc
     2281 gcccatatac ttcgtcagcg agtgcacgac gatgtccgcg ccgtgttcga agggacgcgt
     2341 gaggtacggc gtcggcacgg tgttgtccac gatcagcggt acgccgtgcg cgtgcgcgac
     2401 gtcggcgaag gcggcgaaat cgaccacatt gcccagcggg ttgccgatgg attcgcagaa
     2461 caccgctttg gtgcgcgcgt cgatgcgcgc cgcgacggcc gccgggtcgg tggcatcgac
     2521 gaagcgcact tcgatgccgt attgcggcag ggtatgcgcg aacaggttgt aggtgccgcc
     2581 gtaaagcgtg ctgatggcga tgatgttgtc gccggcctcc gcgatggtca tgaccgagta
     2641 ggtgaccgcc gcctggcccg aggcgagggc gagcgcgccc acgccgcctt ccagtgcggt
     2701 gacgcgctgt tccagcacgt cgttggtcgg attcatgatg cgcgtgtaga tattgccctg
     2761 caccttgagg tcgaacaggt cggcgccgtg ctgcgtgtcg tcgaaggcgt agctcgtggt
     2821 ttgatagatc ggcacggcga ccgccttggt ggtcggttcg gggctgtagc cggcatggat
     2881 tgccagggtt tcaaaacgca tgagcggacc tcctctcgat ttcagagcgt caggggttca
     2941 aatcggaaaa tgcctaatca tggccgatat tgcgcttacc gggtggactc ggctttcgga
     3001 tcgatcaaag ccttgtgaat gcggcggtgc ggcagaccga cgtggtcata gacttcgtcg
     3061 tgtgccgtat agccctgctt gagatagaag cccagggcgg agacgcgcgc atcggcgacg
     3121 atttccctgt agccgcggcg gcgcgcgtcg tcttccagat aggcgagcag cgcgccgccg
     3181 acgcccgatg cgcgcatgcc gggcgacacg gccatctgcc tgagatgcgc gcgtgcgggg
     3241 tcgagcggca gcagatacgc cgtggcgacc agccgttcgc ccgcataggc ggcgtagtgc
     3301 agggcgtccg cctcggcggc gagttgttgc ggcgtgaaat tgagtccgag cggtttgcgc
     3361 agcacctcat gacgcagttc gaccgcttca cggtaggcat cgctgccgtg ggcgatgacg
     3421 gtgaggcgca gcgccgccat ggccagtcag ctcgatgaga gatagcgcat cgcccggtcg
     3481 aggccgtcga gcgtcagggg atacatgcgc ccgtccatga tctgccgcat gagcccgatg
     3541 gagtagcggt agggccacat ctcctccgac tccggattga gccacacggc cttcggataa
     3601 acgtccagca tgcggcgcat ccagacgatg cccggctcct cgttccagtg ttccaccgag
     3661 ccgcccggct cggtgatttc ataggggccc atggtcgcgt cgccgacgaa gatcagcttg
     3721 tagtcgctgc cgtacttgtg cagcagatcc atggtgccga tgcgatcctt gcgccggtag
     3781 gcgttgtcct tccacaccga ttcgtacagg cagttgtgga agtagtagtg ctccagatgc
     3841 ttgaactcgc tcttgcacgc cgagaacagt tcttcgcaca actggatgtg gtcgtccatc
     3901 gaaccgccca cgtcgagcag cagcaggacc ttgaccgcgt tgtggcgctc ggggcgcatt
     3961 tgcagatcga gccagcctgc gtttttcgcg gtcctgctga tggtgctttc cagatcgagt
     4021 tcttcggccg cgccgctacg cgcaaactgt cggagccgcc gcagtgcgac tttgatgtta
     4081 cgcgtgccga gttcgacctc gctgtcgaag tttctgaatt cgcgttcgtc ccacactttc
     4141 accgcgcggc ggtgacgcga ctcgtgctgc ccgatgcgta tgccctcggg gttgtagccg
     4201 taggcgccga agggcgagcg gccggccgtg ccgatccact tgttgccgcc ctggtggcgt
     4261 tccttctgtt cctcgaggcg cttcttaagc gtctccatta gcttgtcgag gccgcccatg
     4321 gcctcgatgg cagccatttc ctcgggcgag agcttgagtt ccttgagccg gcgcagccac
     4381 tcctcgggaa tgagcgcctc ttccagcggg atcgattcca cgccttcgaa gtattcgccg
     4441 aacgcgcggt cgaacttgtc gaagtgggtt tcgtccttca ccagcgcggc gcgggacaag
     4501 taatagaagc cgtcgaccga cggcccgatc acgccccttt ccaaggcttc cagcagcgtc
     4561 aggaattcct tcagcgaaac ggggactttc gctgcgcgca ggcggtaaaa gaaatcaatc
     4621 aacatgaaaa gctttcaccg cagaggcaca gagacacaga ggaaaaccat tttcaatagc
     4681 gggaacccgc atggcgcagt ccgtcgttct tgg
//
LOCUS       DATGOU010000124         5896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_4821137, whole genome shotgun sequence.
ACCESSION   DATGOU010000124 DATGOU010000000
VERSION     DATGOU010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4821137"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..384
                     /locus_tag="VKZ48_07250"
     CDS             121..384
                     /locus_tag="VKZ48_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77680.1"
                     /translation="MSKPNQTPPDAGITDDEPKFPPQTTDPESEFSVDESLKQSFAEE
                     RDKAHHQSASANSKQKQQGGSSSSESEFSVSQRRQESQGDRRD"
     gene            514..1092
                     /locus_tag="VKZ48_07255"
     CDS             514..1092
                     /locus_tag="VKZ48_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HLU77681.1"
                     /translation="MQTPPEIKFHECTRSAWIEDYIRERLQKLDRIADGGLTSAVVAV
                     SGEQFAKTKGNVYGVMINVRLPPNHDLAANKSREIGDIQMQLRPLIKQTFEAIERQLK
                     DTLARRRYDVKAPEFEEPRGMVEKLFADGYGFIRTMGDGREFYFHRNSVLHDDFDRLA
                     IGTEVRFMPQSGEEGPQASSVQIVAKPGANPP"
     gene            1159..2160
                     /locus_tag="VKZ48_07260"
     CDS             1159..2160
                     /locus_tag="VKZ48_07260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008353522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLU77682.1"
                     /translation="MLPLSILDLSPIKAGETAADALRNTLALAQQAERLGYTRFWLAE
                     HHNMPGIASAATSLVIAHVAAGTQSIRVGAGGIMLPNHSPLVIAEQFGTLASLFGDRI
                     DLGLGRAPGADQATARALRRDPFASSDQFPQDVVELQRYFRDESPPNGIRAIPGAGLA
                     IPLWILGSSLFGARLAALLGLPYAFASHFAPDYLDRAVEVYREEFRPSEQCERPYLML
                     TVNVLMADTDEDARRHFTSQQQSFLQVRRGAAMPVPPPVESMDELWSPAERAGVEHAL
                     KYSFVGAPDTASAGLRAFIETYRPDELMVTALIYDQAARLRSLELLAAARTRICSPG"
     gene            2325..4028
                     /locus_tag="VKZ48_07265"
     CDS             2325..4028
                     /locus_tag="VKZ48_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HLU77683.1"
                     /translation="MGRPEKIRLGETLLLQKLISQEQLKLALDEQRRSGRKLGRVLVE
                     HGFVTEDQICEGLSKQFGIPYLNLKFVNVVPAIARKLPEMHARRHRALVLEERKDSYL
                     VGMSDPSDLVAYDEVARILKRDIDLAVVNESLLLQTLDRVYRRTEEISGLARALEQDI
                     GDSVIDLNTLSASASVEDAPVVRLLQTLLEDATQINASDIHIEPQERRLHVRFRIDGM
                     LVLQTEADAKIGPPLVLRLKLMAGLDISEKRLPQDGRFNIRAREQQVDVRVSTMPTQY
                     GESVVLRLLNQSSGTKGLDSLGMPPAILERVRAISKRSSGMMLVTGPTGSGKTTTLYS
                     TLAEINTIEKKIITVEDPVEYRLPGVSQVQINEKIDLSFGRVLRSVLRHDPDVILVGE
                     MRDQETAQIGLRAAITGHLVFSTLHTRDAASTPLRLIDMGAPAYMVSTSLHAVLAQRL
                     VRLVCESCSEPHVLAPQEAAWLEACSESLSSAAGFVHGRGCSHCNGTGFRGRSGVYEL
                     LEMDAALVEAVNRNDPAGFMESAQAMMGGRTLRSHALSLAVAGRTTVAEVMRVASEVE
                     D"
     gene            4028..5254
                     /locus_tag="VKZ48_07270"
     CDS             4028..5254
                     /locus_tag="VKZ48_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HLU77684.1"
                     /translation="MATFEYRGRNARGELVNGVIEASDTGAVADQLFNTGITPVDIRA
                     AASGAAQVNDLLARWRDQVSAVDLMLFARQMHTLMRAGVPIMRALAGLQESATNPAMA
                     RVIADLRTNLDGGRELSAAMRRHPAVFSAFFVSMVRVGEMTGRLEEVFLRLAQHLEFE
                     TEMRDRVKAALRYPTFVVAAMVAALVIVNVFVIPTFAQLYQGFNAELPVLTKALIAFS
                     NFTVNYWALLLACAVGTAIGFKLWIATPNGRYRWDKFKLRIPIAGKIIVKATLARFAR
                     SFALSMRSGVPLVQALTVVSKVVENDYIGVRIEQMRDGVERGESVLRTAVTAGVFSPV
                     VLQMIAVGEETGALDELLFEIAAFYERDVEYDLKTLASQIEPILIVALAVLVLILALG
                     VLMPIWGLGKAMTAAS"
     gene            5257..5802
                     /locus_tag="VKZ48_07275"
     CDS             5257..5802
                     /locus_tag="VKZ48_07275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HLU77685.1"
                     /translation="MRIPLCRMRGFSLVELVVVVVLLGILSAVMFRMYVDYTEQAEQT
                     AMEQVASGMRAALHLRVAGLLARGADDAIGHLAGQNPMDWLADKPHLYAGVFHGVPPV
                     ELAPAPCWYYDSHAREIVYRPLRTTHLEVPAHPPHDIRFKVWVEQGTLPGGEMLAEPL
                     RGIRKLELAPVEAYRWITQSR"
BASE COUNT         1098 a         1904 c         1825 g         1069 t
ORIGIN      
        1 ccaccgctcg cacgacgagg gcgattcgca tcgcccccgt tgccttgtgc agtctttagc
       61 ccagtcggcc ccatttttga ttgccggcac agggcaagcc acggactcag gagccacgcc
      121 atgagcaaac ccaaccagac cccgccggat gccggcataa cggacgacga accgaaattc
      181 ccgccccaga cgaccgatcc ggaatcggag ttttccgtcg acgagtcgct gaagcagtcg
      241 ttcgccgaag agcgcgataa agcgcatcac caaagtgctt ccgccaactc gaaacaaaaa
      301 cagcaaggcg gctcgagcag ttcggagtcg gagttctctg tcagccagcg acgccaggaa
      361 agccagggtg atcgtcggga ttgaacccgg gcgcgctcac ggctgaaaaa gcgacaatgt
      421 gtattatgta aaatcgcgga tccgggccca tgcggcagta ccccgtgcgg cccggcagcg
      481 ccttcccctc tccatccagc acgtcaggcc gccatgcaga ccccacccga aatcaagttc
      541 cacgaatgca cccgcagcgc atggatcgag gactacatac gcgaacggct gcagaaactc
      601 gaccgcatcg ccgatggcgg cctcacgtcg gcagtcgtag ccgtctcggg cgaacagttc
      661 gccaagacca agggcaacgt ctatggcgtg atgatcaatg tccggctgcc gcccaaccac
      721 gatctggccg ccaacaagtc gcgcgaaatc ggcgacatcc aaatgcaact gcgcccgctg
      781 atcaagcaga cattcgaggc catcgagcgc cagttgaagg acacgctggc caggcgccgt
      841 tacgacgtga aggcgccgga attcgaggag ccacgcggca tggtggaaaa actttttgcg
      901 gacggctacg gcttcatccg caccatgggc gacggccggg agttctattt ccaccgcaac
      961 agcgtgctgc acgacgactt cgaccgcctg gcgatcggca cggaagtgcg cttcatgcct
     1021 cagtcgggtg aggaaggccc gcaggcgagc agtgtgcaga tcgtcgccaa gcccggcgcg
     1081 aatccgcctt gaggcttgcc gggccgtggg cagcgggcta gcctgcccgt ctccaccggt
     1141 atgacttcga caccttccat gctcccgcta tcgatcctcg atctttcgcc catcaaggcc
     1201 ggggaaacgg ccgcagacgc gctgcgcaac accctggcgt tggcgcagca ggccgagcgc
     1261 ctcggctata cgcgcttttg gctggccgag catcacaaca tgccggggat cgcgagcgcc
     1321 gcgacatcgc tggtcattgc ccatgtcgcg gcgggtacgc agtccatccg tgtcggcgcg
     1381 ggcggcatca tgctgccgaa ccactcgccc ctggtgattg cggaacaatt tggcacactg
     1441 gcgtccctct ttggagaccg gatcgacctg ggactcgggc gggcgccggg ggcggaccag
     1501 gcgaccgccc gcgcactccg tcgcgatccg ttcgcttcgt cggatcaatt cccgcaggat
     1561 gtggtggaac tccagcgtta tttccgcgac gagtctccgc ccaacggcat ccgcgcgatt
     1621 cccggtgcgg gcttggccat accgctgtgg atactcggct ccagcctgtt cggtgcgcgc
     1681 ctcgccgccc tgctcggcct gccttatgcg ttcgcctcgc atttcgcgcc ggactatctc
     1741 gatcgcgccg tcgaggtcta tcgggaggag tttcggcctt ccgaacagtg cgaacggcct
     1801 tatctgatgc tcacggtgaa cgtgctgatg gccgataccg acgaggacgc gcgccggcac
     1861 ttcacgtcac agcagcagtc cttcctgcaa gtgcgccgcg gcgcggccat gccggtgccg
     1921 ccgccggtcg agtcgatgga cgaattgtgg tcgcccgctg aacgcgccgg cgtcgaacac
     1981 gcgctcaagt attccttcgt cggcgcgcct gacaccgcct cggccggcct gcgagctttc
     2041 atcgagacct atcgcccgga cgaactaatg gtgacggcgc ttatctacga tcaggcagcg
     2101 cgattgcggt cgctcgaatt gttggcggcg gcgcggactc ggatctgttc gcccggctga
     2161 tttcagcgca agaatctgtc cgatattcga cacaactggc gatttttcag caacatatct
     2221 ccttataaaa caaggaaata tgtatcggag ttgaagcgat ctctattgct acctcgagcc
     2281 aataatccta tggttgtgcc ggcaatccga cataggggag acgggtgggc cggccagaaa
     2341 aaattcgact cggggaaacg ctgctgctgc agaagctgat ctcgcaggag cagttgaaac
     2401 tcgctttaga cgaacagcgg cgcagcggcc gcaaactggg ccgcgtactc gtcgaacacg
     2461 gcttcgtcac cgaagaccag atctgcgaag gcctcagcaa gcagttcggc atcccctacc
     2521 tcaatctcaa attcgtcaat gtcgtcccgg ccatcgcgcg caagttgccg gagatgcatg
     2581 cacgccgtca ccgtgcgctg gtgctagaag agcgcaagga cagttacctg gtcggcatga
     2641 gcgatcccag cgacctcgtc gcttacgacg aggtcgcccg catcctcaag cgcgacatcg
     2701 acctggcggt cgtcaacgag tcgctgctgt tgcagacgct cgaccgtgtg taccgccgta
     2761 ccgaggaaat ctccggtctg gcgcgcgcgc tggaacagga catcggcgac agcgtcatcg
     2821 acctcaatac gctttccgcc agcgcaagcg tggaagacgc gccggtggtg cggttgctgc
     2881 agacgctgct ggaggatgcg acgcagatca acgcctccga catccatatc gagccacagg
     2941 agcgccggct gcacgtgcgt ttccgcatcg acggcatgct ggtgctccag acggaagccg
     3001 acgccaagat cggtccaccg ctggtgctgc ggctcaagct gatggcggga ctcgatattt
     3061 ccgagaaacg cctgccgcag gacggccgat tcaacatccg cgcgcgcgag cagcaggtcg
     3121 acgtgcgcgt ctccaccatg ccgacgcaat atggcgaatc agtggtgctg cgtctgctca
     3181 accagtcgtc cgggaccaaa gggctcgatt cgctcggcat gccgcccgcg atcctggaac
     3241 gcgtgcgtgc catctcgaag cgctcgagcg gcatgatgct ggtcaccggt ccgacgggca
     3301 gcggcaagac gaccacgctc tactccaccc tggccgaaat caacacgatc gagaaaaaga
     3361 tcatcactgt cgaggatccg gtcgagtacc ggctacccgg cgtcagccag gtgcagatca
     3421 acgaaaaaat cgacctttcc ttcggccggg tgctgcgctc ggtcctccgt cacgacccgg
     3481 acgtcattct ggtcggcgaa atgcgcgacc aggaaacggc gcaaatcggt ttgcgcgccg
     3541 ccattaccgg tcacctggtt ttttccacgc tgcatacgcg cgacgccgcc agcacgccgc
     3601 tgcggctgat cgatatgggc gcgccggcct acatggtttc gacttcgctg catgccgtgc
     3661 tcgcgcagcg tctggtgcgc ctcgtatgcg aaagctgcag tgagccgcat gttctggcgc
     3721 cgcaggaagc ggcctggctg gaagcgtgca gcgaatcgct gtcgagcgcc gccgggttcg
     3781 tccacggccg cggctgctcg cactgcaacg gcacggggtt ccgcggccgc agcggggtgt
     3841 acgagctgct ggaaatggac gcggcgctgg tcgaagcggt gaatcgcaac gatccggccg
     3901 gattcatgga aagcgcacag gcaatgatgg gcggccgtac gctgcgctcg catgcgctgt
     3961 cgcttgcggt cgccgggcgc acgaccgtcg ccgaggtcat gcgcgtcgcc agcgaggtcg
     4021 aagactagtg gcgacgttcg agtacagggg ccgcaacgca cgcggcgaac tcgtcaacgg
     4081 cgtgatcgag gcgagcgaca cgggcgcggt ggccgatcag ttgttcaaca ccggcatcac
     4141 gcccgtcgac attcgtgctg ccgcgtccgg ggcagcgcag gttaacgatt tgctggccag
     4201 atggcgcgac caggtttcgg cggtcgatct catgctgttt gcgcggcaga tgcatacgct
     4261 catgcgtgcc ggcgtgccga tcatgcgcgc gcttgccggc ctgcaggaat cggcaaccaa
     4321 tccggcaatg gctcgcgtca ttgcggatct gcgcacgaac ctcgacggcg gtcgcgagct
     4381 gtccgcagcc atgcgccggc acccggccgt tttttcagcc tttttcgtct ccatggtacg
     4441 cgtcggcgaa atgaccggcc gactcgagga ggtttttctg cgcctggcgc aacacctcga
     4501 gttcgagacg gaaatgcgcg accgcgtcaa agccgccctg cgctatccga cgtttgtggt
     4561 tgcggcgatg gtcgctgccc tggtgattgt caacgtgttc gtcatcccca cgttcgcgca
     4621 gctctaccag ggtttcaacg ccgaactgcc tgtgctgacc aaagcactca tcgctttctc
     4681 caacttcacg gtgaattact gggcgctgct gctcgcctgt gcggtcggca cggcgatcgg
     4741 cttcaaactg tggatcgcga cgcccaacgg ccgctaccgc tgggacaagt tcaagttgcg
     4801 catccccatc gccgggaaaa tcatcgtcaa agccaccctc gcccgcttcg cacgcagctt
     4861 cgcgctgtcc atgcgcagcg gcgtcccgct cgtgcaggcg ttgacggtcg tgtccaaagt
     4921 ggtggagaac gattacatcg gcgtacgcat cgagcagatg cgcgacggcg tcgagcgggg
     4981 cgagtcggtg ctgcgcactg ccgtgaccgc cggcgtgttc tcgcccgttg tgctgcagat
     5041 gatcgcggtc ggcgaagaaa ccggagcgct cgacgaactc ctgtttgaaa ttgccgcgtt
     5101 ctacgagcgc gacgtcgaat acgacctgaa gactcttgcc tcgcagatcg agccgattct
     5161 cattgtcgcg ctcgccgtgc tggtgctcat tctcgccctg ggcgtgctca tgccgatctg
     5221 ggggcttggc aaggcgatga ccgccgccag ttgacgatgc gtatcccgct ttgccgcatg
     5281 cgcggcttca gtctcgtcga actcgtcgtg gtggtcgtgc tgctcggcat tctcagcgcc
     5341 gtgatgttcc gcatgtacgt cgattacacc gaacaggccg aacagacagc catggaacag
     5401 gttgcttcgg gcatgcgcgc tgcgctgcat ctgcgcgtcg ccggcctgct cgcgcgcggc
     5461 gccgatgacg ccatcgggca tctggccgga cagaacccga tggactggct ggccgacaag
     5521 ccgcacctct acgccggcgt ctttcacggc gtaccgcctg tggaactcgc gccggcaccc
     5581 tgttggtatt acgacagtca tgcacgggaa atcgtctatc ggccgctacg caccactcac
     5641 ctggaggtgc cggctcatcc gccccacgac atccgcttca aggtctgggt cgagcaaggc
     5701 acgctgcccg gtggcgaaat gcttgccgaa ccgctgcgtg gcatcagaaa gctggagctt
     5761 gcgcctgtcg aagcttatcg ctggataact cagtcacgct aagacgcacg gggagaatag
     5821 cgtggcatac gaactggcaa gcttcctgct caagaggccg cagggcactg aaatgtaggc
     5881 agtagtgctt gaggga
//
LOCUS       DATGOU010000125         8471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_1719691, whole genome shotgun sequence.
ACCESSION   DATGOU010000125 DATGOU010000000
VERSION     DATGOU010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8471
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1719691"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="VKZ48_07280"
     CDS             complement(<1..1152)
                     /locus_tag="VKZ48_07280"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLU77686.1"
                     /translation="MPDALNSAPPPHVVIVGGGFAGLWAARQLATAPVRVTLVDRRNH
                     HLFQPLLYQVATAGLSAPAIASPLRHMLQRQTNVAVLLADVERVEPAHRRLQLADGNT
                     LHYDYLLLATGSTHAYFGHDEWAQHAPGLKTLEDALGIRRRLLLAFERAEADRDPEQQ
                     REWLSFIVVGGGPTGVELAGTLAEIARHTLRHDFRSIDPAAARVRLVEAGSRVLGGFP
                     EDLSRKAEAQLHKLGVEVLTEAPVKHIDAEGAQVGDRYIRARTVLWAAGVAGSPLART
                     LGVPLDPAGRVIVGDDLRVPGHPEIFVAGDLAALEQDGKPVPGLAPAAKQMGAYVGRA
                     IAADLRGARIAPFRYKDVGALATIGRKAAVADLGRFKLWGLPAWWFWLLV"
     gene            complement(1314..2084)
                     /locus_tag="VKZ48_07285"
     CDS             complement(1314..2084)
                     /locus_tag="VKZ48_07285"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HLU77687.1"
                     /translation="MAEQKWPDILWLVRHGQSAGNVARDEAEAADRPLIDIAERDMDV
                     PLSDLGRRQAIALGRWFGQMPTEQQPTILLCSPYVRARQTAQYLLEGAGLSPDDLTFH
                     IDERLREKEFGVIDRLTVSGIKLKYPELHEQRMHVGKFYFRPPGGESWCDVILRLRSV
                     LDTITRGYRGERVLIIGHQVIVTCMRYLLERMDEQRVLEVDRKSDVPNCGVTSYEFDP
                     GVGRKGKLVSRLVNFVVPLQEAGEMVTTERDIPAAPKS"
     gene            complement(2244..2507)
                     /locus_tag="VKZ48_07290"
     CDS             complement(2244..2507)
                     /locus_tag="VKZ48_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77688.1"
                     /translation="MAHPNPSWSAETDELYDLEYLARANGVQSEDDLGAVYAHPAVNR
                     QLMSVDEKLYQPSGVATEGPAYENLSLVDAFDDSDLGFDRWDY"
     gene            2795..3031
                     /locus_tag="VKZ48_07295"
     CDS             2795..3031
                     /locus_tag="VKZ48_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77689.1"
                     /translation="MKSRMMPIAAALFALALAGCGQEEAPADPGGRGTMTPPGAAGDA
                     AGNQAPANVTDDVPTQQQLDPVPPENQPGGTTQR"
     gene            complement(3036..3524)
                     /locus_tag="VKZ48_07300"
     CDS             complement(3036..3524)
                     /locus_tag="VKZ48_07300"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HLU77690.1"
                     /translation="MPAPRPDPPPHDTRTRLARLAWLLDNSIRIPGTDFRIGLDAIIG
                     LIPGLGDFAGALLSSYIVGQAWRMGVPKSTLVRMGFNVLVEGVVGAVPLFGDIFDAAW
                     KANHRNVQLLHAHLENPRRAVRASRSFVVLLLAALLLLLLGIGLAAYGLMRGLMALGA
                     SV"
     gene            3712..4701
                     /locus_tag="VKZ48_07305"
     CDS             3712..4701
                     /locus_tag="VKZ48_07305"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HLU77691.1"
                     /translation="MNAPQSAAKKSPLKDVAAEPAYREELKIALEELGRARATLRWME
                     QRHFVLVEAMEEGVVMLDNKGQISTTNAAALGLVGDREAIGRWLWGDDPDRPMAGRHP
                     ATETLLDGRPRTGIEMQLTGPDGVTRWLNVNARAVFDMDTHAVAAVLCSFSDITAHKN
                     LELELERQATRDPLTGVYNRRYIERRLKEEFDRARRSGEPLSLVVFDLDHFKEINDRN
                     GHVTGDRVLKRFADMLTLCLRAHDLVARLGGDEFCAILPGATRTDAQEVVTRCLREVA
                     AVKIDRQGRLPPISGSAGIAQFAGEATPEELIAKADHALYSAKGAGRGRVEVA"
     gene            4709..5134
                     /gene="trxC"
                     /locus_tag="VKZ48_07310"
     CDS             4709..5134
                     /gene="trxC"
                     /locus_tag="VKZ48_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HLU77692.1"
                     /translation="MHVVCPHCHRTNRVPAERLHDAPNCGHCKSPLFDGKPVALDDTN
                     FDRHIGADLPVVVDFWAAWCGPCQMMAPQFERAAAELSPAVRLAKLDTEAAQDIAARY
                     GIRSIPTLIAFRNGRELARQAGAMDAASIVSWVRGVAGA"
     gene            5199..6434
                     /locus_tag="VKZ48_07315"
     CDS             5199..6434
                     /locus_tag="VKZ48_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HLU77693.1"
                     /translation="MSLARLLAHPHRERPLRKMKKNISPLLLTLLLAGLAMVGPFSID
                     TYLPSFPAIAHALAVDVTLVQQTLSVYLLAFSLMMLFHGTLSDTFGRRPVVLVALVVY
                     ICASFAAVWSPNLHWLLMCRALQGMSAGAGVVIGRAIVRDLLSGADAQRALAQVTMVF
                     GIAPAVAPIIGGWLQSVFGWTSVFVFLSLFGMALFGAAYARLPESLPREARQPLHLRT
                     ILGNYAHVLRSGRFLLLAFALGAAFAGFGFYIAAAPHFIMDLLRLPETAFGWLFVPMV
                     TGLITGAWVASRTAHSVIPGRMILHGYLLMGTAVLANLLYHLALPAALPWSVLPLLLY
                     NFGLALVMPSITLRILDLFPTMRGLAASLQSFVQTAVFACVAAIAPLFFGSGLKLAAG
                     MVVAFSISVLCYQATRRSS"
     gene            6528..8246
                     /gene="soxB"
                     /locus_tag="VKZ48_07320"
     CDS             6528..8246
                     /gene="soxB"
                     /locus_tag="VKZ48_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfohydrolase SoxB"
                     /protein_id="HLU77694.1"
                     /translation="MQRREFLQVLAAAAACGFQLDAFAAAGRDKFYDLPRFGNVSLLH
                     ITDTHAQLLPIYFREPAVNLGVGEAAGRPPHLVGEHLLRHFGIAPNTREAHAFTYLDF
                     AEAARRYGRVGGFAHLATLVKQLKASRPGALLLDGGDTWQGSATALWTKGQDMIDASK
                     LLGVDVMTGHWEFTYGAERVQEVVQKDFAGNIDFVAQNVRTLDFDEEVFKPYIIREIN
                     GVPVAIIGQAFPYTPIANPRYFVEEWQFGVQDDRLQGYVDEARGKGAQVVVVLSHNGM
                     DVDLKMAGRVRGIDAILGGHTHDGVPQPVVVDNDGGRTLVTNAGSNGKFVGVLDFEVK
                     NKRVVDYRYRLVPVFADLLPADAAMQKHIDSVRAPYRAKLEEVLAVSEDLLYRRGNFN
                     GSFDQVILDGLMAVKDAEIAFSPGFRWGTTILPGQPITRERLMDQTAITYPYTTVTMM
                     SGEMIKTILEDVCDNLFNPDPYRQQGGDMVRVGGLEYSCNPYADIGKRIGDMRLRGKA
                     IDPAKTYKVAGWAPVAPGAEGEPIWEVMEQYLRAEKVIKSRPLNQPKLIGVKADDPGI
                     GEMFKS"
BASE COUNT         1434 a         2736 c         2809 g         1492 t
ORIGIN      
        1 caccagcaac cagaaccacc acgccggcag gccccagagc ttgaagcgcc caagatcggc
       61 gacggcggcc ttgcgcccga tggtggcgag cgcaccgacg tccttgtagc ggaagggcgc
      121 gatccgtgcg ccgcgcaggt cggcggcgat cgcgcggccg acgtatgcac ccatctgctt
      181 ggccgccggt gccaggccgg ggaccggctt gccgtcctgt tccagcgcgg ccagatcgcc
      241 ggcgacgaag atttccggat ggcccggcac gcgcaggtcg tcgccgacga tgacgcggcc
      301 ggccggatcg agcggtacgc cgagcgtgcg cgccagcggc gagccggcca cgccggcggc
      361 ccacagcacg gtgcgcgcgc gaatgtaacg gtctccgacc tgtgcgcctt cggcgtcgat
      421 gtgctttacc ggtgcttccg tgagcacctc gacaccgagc ttgtgcagtt gcgcctcggc
      481 cttgcgtgac aggtcttccg gaaagccgcc gagcacgcgc gacccggctt cgaccaggcg
      541 cacacgtgca gcggccggat cgatgctgcg gaagtcgtga cgcaaggtat ggcgagcgat
      601 ctccgccagc gtaccggcca gttccacgcc ggtgggcccg ccgccgacga cgatgaagct
      661 gagccattcg cgctgctgct cgggatcgcg atcggcctcg gcgcgctcga aggcgagcag
      721 cagacggcga cggatgccga gcgcgtcctc cagcgtcttc agccctggcg cgtgctgcgc
      781 ccattcatcg tggccgaaat aggcgtgggt cgagccggtg gccagtaaca ggtagtcgta
      841 gtgcagggtg ttgccgtctg ccagttgcag ccggcgatgc gccggctcca cacgctcgac
      901 gtccgcgagc agcacggcga cgttggtctg acgttggagc atgtgccgca acggcgaagc
      961 gatggccggc gccgacaatc ctgctgtcgc aacctgatag agcagcggct ggaagaggtg
     1021 atgattgcga cggtcgacga gcgtcacgcg caccggtgcg gtggccagtt ggcgcgccgc
     1081 ccacaagcct gcgaagccgc ctccgacgat caccacatgc ggcggcggtg cgctgttgag
     1141 ggcgtcaggc atggcgatcg ccgcgattgc agggcgtgtt gccctcgcgc tcgaccgttc
     1201 ggggtcgctt cagaacgatt gaaagtggtg ttgcgcgtcc gaccatacgg gcctcctgtg
     1261 ttcatcgcat gcgccttcat gcaacaaacg ccgcccgcaa cgcggacggc gagtcaggac
     1321 ttcggtgcgg cggggatgtc gcgttcggtg gtgaccattt cgcccgcttc ctgcagcggc
     1381 acgacgaagt tcaccagacg ggaaaccagc ttacccttgc ggcccacgcc gggatcgaac
     1441 tcgtaggagg tcacgccgca gttgggcacg tcgctcttgc ggtcgacttc gagcacgcgc
     1501 tgctcgtcca tgcgttcgag caggtagcgc atgcaggtga cgatcacctg atggccgatg
     1561 atcagcacgc gctcgccgcg gtagccgcgg gtgatggtgt cgagcacgct gcgcagccgc
     1621 aggatgacgt cgcaccagct ttcgccgccg ggcgggcgga aatagaactt gcccacgtgc
     1681 atgcgctgct cgtgcagttc cggatacttg agcttgatgc cggaaacggt gagccggtcg
     1741 atgacgccga actccttttc ccgcaggcgt tcgtcgatgt ggaaggtcag gtcgtcgggc
     1801 gacaggccgg cgccttcgag caggtactgc gccgtctggc gggcgcgcac atagggggaa
     1861 cacaggagga tggtgggctg ttgttcggtc ggcatctgtc cgaaccagcg accgagcgcg
     1921 atggcctggc ggcggcccag atcggacaaa gggacatcca tatcgcgctc ggcgatatcg
     1981 atcagcggac ggtctgccgc ctccgcttca tcgcgtgcaa cgtttccggc gctctggccg
     2041 tgtcgcacca gccagagaat gtcgggccat ttctgttccg ccatgaatct cgattgttct
     2101 atgcgcccgc tgccagcagc gagacggtgg ggatgcagtc gagtctaacc cgacacgcgg
     2161 gccttgtaat gcccgaatga ctctcaagga cgtgcttgcc gcggtcggtg cggcggcgcc
     2221 gaccgcgttt catccggcga cgctcagtaa tcccagcggt cgaagcccag gtcgctgtcg
     2281 tcgaacgcat cgacaagcga caggttctcg taggcggggc cctcggtcgc gacgccggaa
     2341 ggctggtaga gcttttcgtc caccgacatc agttggcgat tgacggccgg gtgtgcataa
     2401 acggcgccca ggtcgtcctc cgactgcacg ccgttggcgc gcgccaggta ctccagatcg
     2461 tacagttcgt ccgtttccgc ggaccaggaa gggttggggt gtgccatcag tggttcgact
     2521 ccatttggtg tctgcctgcg gtgcgcacaa aacgtgcgcc gccacctcgt catttgaaga
     2581 gaaacggcgg ttcctgcata ggccggagcg cttcaaacgc ggccattttg cgcaatcgaa
     2641 aacggccgct cgtgattcag gtcaagactt ggtgacgccg cagcttcgtt ctttaaaaaa
     2701 agacgcgtaa agcggttcgg agttccctcg gatttgaaat gcgggaccgc gcattgcagg
     2761 cagtcgggtt cgacccaacg agaggaagca gcctatgaag agccgaatga tgccgattgc
     2821 cgccgccctg tttgcgcttg cgctggccgg atgcggtcag gaagaggcgc ctgccgatcc
     2881 gggcgggcga gggaccatga cgccgcccgg cgcggctggc gacgccgccg gcaatcaggc
     2941 gcccgcgaac gtgaccgacg acgtgcccac acaacagcaa ctcgacccgg ttccgccgga
     3001 gaaccagccg ggcggcacga cgcagcgctg aactctcaga ccgacgcgcc gagcgccatc
     3061 agcccgcgca tcaggccgta ggccgccagg ccgatcccca gcagcaggag cagcagcgca
     3121 gcgaggagca gcaccacgaa gctgcggctt gcacgcaccg cccggcgcgg attctccagg
     3181 tgggcgtgca acaactgcac attgcggtgg ttggccttcc aggcggcgtc gaaaatgtcg
     3241 ccgaacagcg gcacggctcc gaccacccct tcgaccagta cattgaaacc catgcggacg
     3301 agcgtgcttt tgggcacgcc catccgccag gcctgcccga cgatgtagct ggacagcaag
     3361 gcgccggcga agtcccctaa accggggatc aggccgatga tcgcatccag tccgatacgg
     3421 aagtcggtcc cggggatgcg gatggagtta tcgagcagcc aggcgagtcg cgcaaggcga
     3481 gtacgagtgt cgtgaggcgg cgggtcggga cgcggggcgg gcatgtgccg gtcaatgcaa
     3541 gcgactcacc cgcgattcgc cccagcgagc gcctgggagc ggaaaatgac cgcttatccg
     3601 gccgattcgt ggaaactttc acgttcgggg cgagctcaat gtttcaatgc ggacttatgg
     3661 gtgcatagct tgcctgagcc ccgcgacact accgtttgca ggagcagcat catgaacgcc
     3721 ccacaatcgg ccgcgaagaa gtcgcccctc aaggacgtcg ccgccgagcc cgcttatcgt
     3781 gaggaactga aaatcgcatt ggaggaactc ggccgcgcac gcgccacgct gcggtggatg
     3841 gagcagcgtc atttcgttct ggtggaggcg atggaagagg gtgtggtgat gctggacaac
     3901 aagggccaga tttccaccac caatgccgct gcgctcggtc tggtaggcga ccgcgaggcg
     3961 atcgggcgct ggctgtgggg cgacgacccc gaccggccca tggccgggcg ccaccccgcc
     4021 accgagacac tgctcgacgg ccgtccgcgg accggcatcg aaatgcagct caccggccct
     4081 gacggcgtca cccgctggct caacgtcaat gcccgcgccg tgttcgatat ggacacccat
     4141 gcggtcgctg ccgtgctgtg ctccttttcg gacatcaccg cgcacaagaa cctcgaactg
     4201 gaactggagc gtcaggctac gcgtgacccg ctcaccggcg tttacaaccg gcgctatatc
     4261 gagcggcgcc tgaaggagga attcgatcgc gcccgtcgtt cgggcgagcc gctgtcactg
     4321 gtggtgttcg atctcgatca cttcaaggag atcaacgacc ggaacggtca cgtcactggc
     4381 gatcgggtgc tgaaacgctt cgccgacatg cttacgctct gcctgcgggc gcacgacctg
     4441 gttgcccggc tgggcggtga cgaattctgc gccatcctgc ccggcgcgac gcgcaccgat
     4501 gcgcaggagg tggtcacgcg ctgcctgcgc gaagtggcag cggtgaaaat cgaccggcag
     4561 ggacgcctgc cgcccatttc cggctcggcc ggcatcgcgc agttcgccgg ggaagccaca
     4621 ccggaagaat tgatcgccaa ggccgatcat gcgctctatt cggcgaaggg cgccgggcgc
     4681 ggccgtgtcg aggttgcctg aggcaagcat gcacgtcgtc tgcccgcatt gccatcgcac
     4741 caatcgcgtc ccggccgagc gactgcatga cgcgccgaac tgcggccatt gcaagagccc
     4801 gttgttcgac ggcaagcccg tcgcgcttga cgacacgaat ttcgatcgcc atatcggcgc
     4861 cgaccttcct gtcgtcgtcg atttctgggc ggcatggtgc ggcccctgtc agatgatggc
     4921 gccgcaattc gagcgcgccg ctgccgaact ctcgccggcg gtgcggctgg cgaaactgga
     4981 caccgaggcg gcgcaggaca tcgccgcacg ttacggcatc cgctccattc ccacactcat
     5041 cgcctttcgc aacggtcgcg aacttgcgcg ccaggcgggc gccatggacg cggccagcat
     5101 cgtgagctgg gtgcggggcg tcgccggcgc ctgattccat ttccgtactc atccttctgc
     5161 cggctgctcc tgtaccgtct ccggcaacgt agaatcgtgt gtcgcttgcg aggcttcttg
     5221 cacacccgca tcgcgagcgg cctctacgga aaatgaaaaa gaacatcagc ccgcttctgc
     5281 ttaccttgtt gctcgccggg ctggcgatgg tcggcccgtt ttccatcgac acctacctgc
     5341 cgtcttttcc ggccatcgcg cacgcgctgg cggtggacgt gacgctggtg cagcagacgc
     5401 tcagcgtcta tctgctggcc ttctcgctga tgatgctgtt tcacggcacg ctgtccgaca
     5461 ccttcggccg gcggccggtg gtgctggtgg cgctggtggt ctacatctgc gcctctttcg
     5521 cggcagtgtg gtcgccgaat ctgcattggc tgctaatgtg ccgcgcgttg caggggatgt
     5581 ccgccggcgc gggggtggtg atcggccgcg ccatcgtgcg cgacctactc tccggcgcgg
     5641 atgcgcagcg tgcgctggcg caggtcacca tggtgttcgg catcgcgccg gccgtggcgc
     5701 cgatcatcgg cggctggctg caaagcgtat tcggctggac gtcggtgttc gtgtttctca
     5761 gcctgttcgg catggcgctt ttcggcgccg cgtacgccag actgcccgaa tcgctgccgc
     5821 gcgaagcgcg acagccgctg catctgcgca ccatcctagg caattatgcg cacgtgctgc
     5881 gaagcggccg ctttctgctg ctggcctttg cgctgggcgc cgccttcgcc ggcttcggtt
     5941 tctatatcgc cgctgcgccg catttcatca tggacttgtt gcggctgccc gaaacggctt
     6001 tcggctggct gttcgtgccc atggtgaccg ggctcatcac cggcgcgtgg gtggcgtcgc
     6061 gcacggcgca cagcgtcata ccgggtcgca tgatcctcca cggctacctg ctcatgggca
     6121 ccgcagtgct ggcgaatctg ctttatcacc tcgcgttgcc ggcggcgttg ccctggtcgg
     6181 tgctgccgct gctgctgtac aacttcggtc ttgcgctggt gatgcccagc atcacgctgc
     6241 gcatcctgga tctctttccg acgatgcgcg ggcttgccgc ttcgctgcaa tccttcgtgc
     6301 agacggcggt attcgcctgt gtggcggcga tcgcgccact gtttttcggc agcggcctga
     6361 aactcgcggc gggtatggtc gtcgccttca gcatcagcgt cctgtgctat caggcgacgc
     6421 gacgttcctc ctgaacggca tatcgggcgg gcgagcgtag taaagtagcg cccttgtgcc
     6481 gcgccgaaac ttccgcgctg gcgccttcca tcgctaccga atctcctatg caacgacgcg
     6541 aattcctcca ggtgctcgcc gcggctgccg cctgcggctt ccaactcgac gccttcgccg
     6601 ccgccggccg cgacaagttc tacgatctgc cgcgcttcgg caatgtcagc ctgctgcaca
     6661 tcaccgacac gcatgcgcaa ttgctgccca tttattttcg cgaaccggcc gtcaatctcg
     6721 gcgtcggcga ggcggccggc cggccgccgc atctggtggg cgaacatctg ctgcgccact
     6781 tcggcattgc gccgaatacg cgcgaggcac atgccttcac ctatctcgat ttcgcggagg
     6841 cggcgcggcg ctacggccgt gtgggcggtt tcgcgcatct ggccacgctg gtgaagcagc
     6901 tcaaggcctc gcgcccgggc gcactgctgc tggatggggg cgatacctgg cagggctcgg
     6961 ccaccgcgct ttggaccaag gggcaggaca tgatcgacgc ctccaagttg ctcggcgtgg
     7021 acgtgatgac cggccattgg gaattcacct acggcgcgga gcgggtgcag gaagtcgtgc
     7081 agaaggattt cgccggcaac atcgacttcg tcgcgcagaa cgtccgcacg ctcgatttcg
     7141 acgaggaggt gttcaagcct tacatcattc gcgagatcaa cggcgtcccg gtggcgatca
     7201 tcgggcaggc cttcccgtac acgcccatcg ccaatccgcg ctacttcgtc gaggaatggc
     7261 agttcggcgt gcaggacgac cggctgcagg gctacgtgga cgaagcgcga ggcaagggtg
     7321 cgcaggtcgt cgtcgtgctg tcgcacaacg gcatggacgt cgatctcaag atggcggggc
     7381 gggtgcgcgg catcgacgcg atcctgggcg gccacacgca cgacggcgtg ccgcagccgg
     7441 tcgtcgtcga caacgacggc ggccggacgc tggtcaccaa tgccggctcg aacggcaagt
     7501 tcgtcggcgt gctggatttc gaggtgaaga acaagcgcgt cgtcgattac cgctatcgcc
     7561 tggtgccggt attcgccgat ctgctgccgg ccgacgcagc gatgcaaaag cacatcgaca
     7621 gcgtgcgcgc gccttatcgg gccaagctgg aggaggtgct cgcggtaagc gaagatctgc
     7681 tgtaccggcg cggcaatttc aacggcagct tcgaccaggt gatactcgac ggcctcatgg
     7741 ccgtgaagga tgccgagatc gcgttctcgc ccggttttcg ctggggcacg accatcctgc
     7801 ccggccagcc gatcacgcgc gagcgcctca tggatcagac cgccatcacc tatccgtaca
     7861 caacggtcac gatgatgtcg ggcgagatga tcaagaccat cctcgaagac gtgtgcgaca
     7921 acctgttcaa ccccgaccct tatcgccaac agggcggcga catggtgcgt gtgggtgggc
     7981 tggaatacag ctgcaacccc tatgcggaca tcggcaagcg catcggcgac atgcgcctgc
     8041 gcggcaaggc catcgatccg gccaagacct acaaggtcgc cggctgggcg ccggtggcgc
     8101 cgggcgcgga aggcgagccg atctgggagg tgatggagca atacttgcgc gcggaaaagg
     8161 tgatcaagtc gcgaccgctc aatcagccga agctgatcgg cgtgaaggcg gacgatcccg
     8221 gaatcggcga gatgtttaag tcgtagtggc gaggcaagct ttccatcgtc attccgggca
     8281 agctgcgaaa cagcgcgatc cggaatccat tgcacgagta aaagctatgg atgccggatc
     8341 aagtccggca tgacaacgcg gcggcagggc gcttacaacc gccattcggc gatcatgccc
     8401 accgaccagc tgcgcccggg cgcgggttcg aaatagcgac cgaccgactg gttcacgatg
     8461 accgagccgg c
//
LOCUS       DATGOU010000126        13295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_6171947, whole genome shotgun sequence.
ACCESSION   DATGOU010000126 DATGOU010000000
VERSION     DATGOU010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 13295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13295
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6171947"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VKZ48_07325"
     CDS             <1..1063
                     /locus_tag="VKZ48_07325"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HLU77695.1"
                     /translation="AVVVLDADDAANAFDQLSGEMHSSTHSALHGATGFAANVPLNHL
                     RTNLNAPLQPGAPIAQAGDAPIPASALPQPATLPLWVEAVGNWQHLDGDSNAASVDHD
                     TGGLFVGGDHRLANGWHLGGAFGYTDSRIRVSDRRSKADADSYSLALYGGKAFQQQNG
                     TLNVMFGGAYTWHSVDAKRAVTIGGATQHLSADYDAHTTQFFGEVGYAFPVTRNTTLE
                     PFAGLAWRELRSESFREKGGSAALQASTNSNDVTTTTLGLRTKTAIKLNGHDASLNAT
                     LGWRHAEGDVTPTSRFAFQGGESFTVAGAPIARDAALVEVGASMMLSRNASLGISYAG
                     QYGEGNRDHTGRISLRWRF"
     gene            complement(1097..1894)
                     /gene="otsB"
                     /locus_tag="VKZ48_07330"
     CDS             complement(1097..1894)
                     /gene="otsB"
                     /locus_tag="VKZ48_07330"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HLU77696.1"
                     /translation="MSGTSERDLPDALKAFDAIEARLADRRPFLCLDYDGTLTPIVAQ
                     PELAVIDLDARATLGEVGRVCPTAIVSGRALEDVRRLVGVPWLYYAGNHGFEIEGHGL
                     SLDVGREYEALMAEVRDALAPRLADMPGILLEDKGCTLSVHYRHASERVLPAFYSAVD
                     TLLIERPRLAGRMGKKVLEIRPAVDWHKGRAVRWLHERIDRDAVPIFIGDDLTDEDAF
                     EVIADDGIGVLVSETPRMTKAHFRIPDVDAVYRLLARIGAHAVRAAA"
     gene            complement(1881..3569)
                     /locus_tag="VKZ48_07335"
     CDS             complement(1881..3569)
                     /locus_tag="VKZ48_07335"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HLU77697.1"
                     /translation="MPERTLIVSNRLPVTLNRKEDGSWEARPSSGGLVSALEPVLTRC
                     GGVWIGWPGASDCPQAEIERALAAMSAGYRCRAVMLAPEEVEGFYQGFSNEIIWPLFH
                     DLFTNCNFDPDYWQAYRRVNRKFARAVAQEHREGDYIWVHDYHLMELARELKRLGVAA
                     RTGFFLHIPFPPLEMFLKLPWRFEILRSLLAFDTLGFQTARDQHNFLACAHALFDIRL
                     EGSGRLVRVRVRDDSLETWCESAVESRRAVTPDELPKMGEFKAAVVPLRGDARTPAEG
                     ACSLSPVSHAMVVPMPRNPVDEPASTQRARTVQVGAFPIGIDYDAIAAAAARDECRAK
                     AAVLRTDLARSQLMLGVDRLDYTKGLINKLTAFRNALQRYPDLHQKVTLVQHVVPSRS
                     DIPEYRRLRQELEQCVGEINGEFTASGWVPVHYMYHSLDFEDLIAYYLAADIALVTSL
                     KDGMNLVAKEYCAAQIKEDGVLILSEFAGAAAQLQAGALLVNPHDIAGMADAIHQAVH
                     MPYGERQQRMRALRRTVRTEDVYSWADAFLDNAAPPPAPRIPRRREDYRPHVGH"
     gene            complement(3911..5218)
                     /locus_tag="VKZ48_07340"
     CDS             complement(3911..5218)
                     /locus_tag="VKZ48_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006390937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HLU77698.1"
                     /translation="MKPLLAGLIALVTLAWAATPLDPAAATPWLVRQEALYLTGLWSI
                     ALLSLAMVLATRPTWLERPLGGMDRIYRTHKWAGILAVAFAALHWLIEMSDDLIKSLW
                     GRAGRVSIEHPGGFFEIMRELGEDLGEWAIYALLAMLVLTLWKRFPFSVWRYLHKAMP
                     VLYLMLVVHAAFLAPVDYWQQPIGILLALLIAAGSAASVQSLSGMIGSRRRAGGVIES
                     VREPGAGITEVTCRLDQKWRGHRAGQFAFVTFDRLEGHHPFTIASADSSDRRVTFQIK
                     ALGDYTNKLGSRLHPGRTMRIEGPYGRFDFRRSRRGAAQIWIAGGVGVTPFLAWLESL
                     QGAPAQAPRVDLYYCTRAPDHDPFVMRLKALCATLPSVRLHINGDDGAPLTAELLYAS
                     HSRTKPAEIWFCGPRGLADALRSGLRRLGMRATPFHQEAFEMR"
     gene            complement(5286..5618)
                     /locus_tag="VKZ48_07345"
     CDS             complement(5286..5618)
                     /locus_tag="VKZ48_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006222666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HLU77699.1"
                     /translation="MRTSTSFLAVVALSTGLVAGGTLLAPAIAQNADAGQATASTSKR
                     LTIGEVHDKLIAQGYRDIDEIEREDRGFEVKAVDAEGRRVKLEVDAVTGEVLGTRTKS
                     DKRDRAMQ"
     gene            complement(5735..6229)
                     /locus_tag="VKZ48_07350"
     CDS             complement(5735..6229)
                     /locus_tag="VKZ48_07350"
                     /inference="COORDINATES: protein motif:HMM:NF021165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diheme cytochrome c"
                     /protein_id="HLU77700.1"
                     /translation="MRIPVRALAIGAIALSFSALLYSRAQAGSDHYFPPVKDPLTLQE
                     CGSCHLAYPASMLPTASWKRMMADLQNHFGDDASVDAETAAAIARYLVANAGDTGGAR
                     YGRKLLRDVRANEAPLRITELPRWLHEHDEVSQREWTSKEVGSKANCGACHIDADKGY
                     FDDD"
     gene            complement(6242..6658)
                     /locus_tag="VKZ48_07355"
     CDS             complement(6242..6658)
                     /locus_tag="VKZ48_07355"
                     /inference="COORDINATES: protein motif:HMM:NF020652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1924 domain-containing protein"
                     /protein_id="HLU77701.1"
                     /translation="MTKSAYAMIAVCALGFAAGVSAGPREDLLDRYAREAKAAEPAFD
                     NFSAARGETLHRTPFARGKPDTLACTSCHEKDARVAGRTRTGKAIEPMAVSVSPKRYT
                     DPAKVEKWFKRNCNEVLGRECTPREKGDWLAYMMKQ"
     gene            6817..7149
                     /locus_tag="VKZ48_07360"
     CDS             6817..7149
                     /locus_tag="VKZ48_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HLU77702.1"
                     /translation="MRRLAWIVTLLLAVSAAQADDARDHDRAREALEAGEVMPLPRIL
                     DRVERDYPGRVLEVELERDDGRWLYEIKLLQPDGRVLKLDVDAADARVLKVKGREHHE
                     RRDERRSR"
     gene            7149..7811
                     /locus_tag="VKZ48_07365"
     CDS             7149..7811
                     /locus_tag="VKZ48_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLU77703.1"
                     /translation="MRILVVEDEHVLAAQLCEALSEAGYAVDVAHNGLDGYHLGAEES
                     VDAVVLDLGLPHMDGITMLTKWRAAGRDMPVLILTARDDWHQKVAGIDAGADDYVTKP
                     FHMEELLARVRALIRRAGGHASATLHCGPLTLDTRAGRLTIEGRAVPLTSHEYRVLAY
                     LMHHTGEVVSRTELTEHIYAQDYDRDSNTIEVFIGRLRKKLPPGMIETVRGLGYRLVD
                     GK"
     gene            7808..9166
                     /locus_tag="VKZ48_07370"
     CDS             7808..9166
                     /locus_tag="VKZ48_07370"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HLU77704.1"
                     /translation="MNLRDSLRLRLLAGSLVWIVVTLTVTGFVLTDLFRQHVAARFEA
                     ELQGHLDQLAAVFEFAEDGMPRLRAELSDPRLRLPYSGLYWQIDRAANGAAVGVLRSR
                     SLWDSTLTVPADALADGQAHVHRVDGPDAAQLIMVERMIRPASRPETAYRLIVAADER
                     AMTEPVAAFVGLLVRALAILAAGLVALVALQLYLGLMPLRRMREALATVRAGRSRQLA
                     GRYPQEVQPLVDELNTLLRHDAEVVARARTQAGNLAHAVKTPLAILANAASREQGEFS
                     RLVAEQAAIARRQVDYHLARARAAAATLVPGMHTPLRPALEGLVRVMRRLHAERELEI
                     DVAQVPDKLGFAGEEQDLHEMLGNLLDNACKWARQRVVVNASLEGRTLAVRIDDDGPG
                     LAPAEREAVFARGARADEQVPGSGLGLAIVRDLAHLYGGDVHLDVAPQGGLRVTLTLP
                     AA"
     gene            9283..9744
                     /locus_tag="VKZ48_07375"
     CDS             9283..9744
                     /locus_tag="VKZ48_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HLU77705.1"
                     /translation="MSETVTVTITQLDNYRFNVDFGAGLPQLVADEPAPMGAGTGPDP
                     ARLLLSAVANCLSASLVFAFSKFKQDPGGITTTATAHIERNERNRLRVGRIDVTIRLG
                     RAGGELEHLERLLGQFEDFCTVSQSVQSGVPIAVQVEDTAGTVLKPGGVTV"
     gene            9779..10252
                     /locus_tag="VKZ48_07380"
     CDS             9779..10252
                     /locus_tag="VKZ48_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77706.1"
                     /translation="MLIPKTLTVAIVAAILFGTSVGVLAASDHAHHGGGVAHLQLNEG
                     RKWPTDAPLRQGMGNMQAAMSQALPQIHHGKLDAAGYAALAARLEREMAYVVANCKLD
                     PDADAQLHLVLAQLGAGLEQMKAGGDRRAGAVSVVDALDAYGTHFDHPGWHALRH"
     gene            10397..10843
                     /gene="ccmE"
                     /locus_tag="VKZ48_07385"
     CDS             10397..10843
                     /gene="ccmE"
                     /locus_tag="VKZ48_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HLU77707.1"
                     /translation="MKPRHKRIALIAGGLAALGFAAALVLNAFQSNLVFFFSPSQVVA
                     KEAPLDKTFRIGGMVEDGSLKRLPDGLSVEFKVTDTAHAIPVTYTGILPDLFKEGKGV
                     VAQGRLGPDGMFRATEVLAKHDENYMPPDAAHAIEQAQKAQQTLQQ"
     gene            10840..12810
                     /locus_tag="VKZ48_07390"
     CDS             10840..12810
                     /locus_tag="VKZ48_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HLU77708.1"
                     /translation="MIPELGHFALIVALMVALAQGVLPIVGAARGTVSWMEMARPAAQ
                     VQFALVAFAFFCLAWSFAHNDFSVLNVASNSNSQLPLHYRVAAAWGSHEGSLLLWVFM
                     LGMWTCAVSLFSRHLPLEMVSRVIGVMGLVSVGFLLFMLLTSNPFERLLPAAPDGRDL
                     NPLLQDPGMVIHPPLLYMGYVGFSVAFAFAIAALLSGKLDATWARWSRPWTTLAWCFL
                     TLGIAVGSWWAYYELGWGGWWFWDPVENASFMPWLVGTALIHSLAVTEKRGSFKSWTV
                     LLAILTFSLSLLGTFLVRSGVLTSVHAFATDPARGVFILAFLVLVIGSSLALFAWRSP
                     KVGLGGKFDMVSRESTLLMNNVLLVVAAGTVLLGTLYPLFLDALNLGKISVGPPYFEA
                     VFVPLMTPVVFLMGIGPLTRWKKAEIPDLAKRLRWAAGVSLVTALLAPLAGGDWTAMS
                     AFGYLLAFWVVATCVVSLKERIRNFRGGLWARLAAQPRSYYGMLLAHLGIGVFIVGVT
                     SVMSFEVERDVRMDVGDSVVVGPHSFRFDGVRELQGPNYTGAEGIVTVSRNGKTVAVL
                     HPQKRLYTVQQMPMTEAAIDSGLLGDIYVSLGEPVGDGAWAVRVYRKPFVTWIWGGCV
                     LMALGGFIALADRRYRVRVRRTEPAAAVGEAA"
     gene            12819..>13295
                     /locus_tag="VKZ48_07395"
     CDS             12819..>13295
                     /locus_tag="VKZ48_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HLU77709.1"
                     /translation="MSKYLLPLGVFITVAVFLFYGLSLNPREVPSPLVGKPAPAFQLP
                     VLHSPDKTFTPKEMAGKVWLLNVWASWCVACRDEHPLMVELSRRKIVPVVGLNYKDQP
                     DDAKKWLRHFGDPYLTSVSDLEGRVGIDFGVYGVPETFVIDKDGIIRHKHIGPITED"
BASE COUNT         2211 a         4207 c         4433 g         2444 t
ORIGIN      
        1 tgccgtggtt gtgctcgatg ccgacgatgc ggccaacgcc ttcgatcagc tctcgggcga
       61 aatgcattcg agtacgcatt cggcgctgca cggggcgacc ggcttcgctg ccaacgtgcc
      121 gctcaatcat ctgcgcacga accttaacgc gccgttgcaa cccggtgcgc ccatcgcgca
      181 agcaggcgat gcaccgattc cggcttcggc cttgccgcaa ccggcaacac tgcccctgtg
      241 ggtcgaagcg gtcggcaact ggcagcatct cgatggcgac agcaatgccg caagcgtcga
      301 tcacgacacc ggcggactct tcgtcggcgg cgatcatcgt cttgcgaacg gctggcatct
      361 gggcggtgcg ttcggttaca ccgacagccg cattcgcgtg agcgatcgcc gttccaaagc
      421 cgatgccgac agctacagtc ttgcgctcta cggcggcaag gcctttcaac aacagaacgg
      481 cacgctgaat gtgatgttcg gcggcgcgta cacctggcac agcgtcgatg cgaagcgcgc
      541 cgtcaccatt ggtggtgcaa cccaacatct cagcgccgac tacgacgcgc acaccacgca
      601 gttcttcggc gaagtgggtt atgcgtttcc tgtgacgcgc aatacgacgc tcgaaccctt
      661 cgccggcttg gcgtggcgtg aattgcgcag cgagtccttc cgcgagaagg gcggcagcgc
      721 agcgctgcag gcgagcacca acagcaacga cgtcaccacg acgacgctgg gcctgcgcac
      781 gaagacggcc atcaagctga acggccacga cgcgagcctc aacgccaccc tcggctggcg
      841 ccatgccgaa ggcgatgtca cgccgacctc gcgctttgcc ttccagggcg gcgagtcctt
      901 caccgtagcc ggtgcgccga tcgcgcgcga tgcggcgctc gtcgaagtgg gggcgagcat
      961 gatgctgtcg cgcaatgcgt cactgggcat cagctacgcc ggtcagtacg gcgaaggcaa
     1021 ccgcgatcac accgggcgca tcagcctgcg ctggcggttc tgatgtgctt cggcgcgctc
     1081 gcggcggcgg gttcgctcaa gcggccgctc tgaccgcatg cgcgccgatg cgcgcgagca
     1141 gtcggtaaac cgcatccacg tccgggatac ggaagtgggc tttggtcatg cgcggtgtct
     1201 cgctcacgag aacgccgatg ccgtcgtcgg cgatgacttc gaaagcgtct tcgtcggtga
     1261 ggtcgtcgcc gatgaaaatc ggcaccgcgt cccggtcgat gcgctcgtgc agccaccgca
     1321 cggcgcggcc cttgtgccag tccactgccg gacggatttc cagcactttc ttgcccatgc
     1381 ggccggcaag tcgcggccgc tcgatgagca gcgtatcgac agccgagtag aacgccggca
     1441 gcacccgttc ggaagcatga cgatagtgga cggacagcgt gcagcccttg tcttcgagca
     1501 ggatgcccgg catgtcggcg agccgtggtg cgagcgcgtc gcgcacctcg gccatcagcg
     1561 cctcgtattc gcgccccaca tcgagcgaca ggccgtgtcc ctcgatttcg aagccgtggt
     1621 tgccggcgta gtaaagccac ggcacgccga ccagccggcg tacgtcctcc agcgcgcggc
     1681 cactcacgat ggcggtggga caaacgcgcc ccacttcgcc cagcgtcgcg cgcgcatcga
     1741 gatcgatcac cgccagttcg ggctgggcga cgatgggcgt gagcgtgccg tcgtagtcga
     1801 ggcaaaggaa aggacggcga tccgccaacc gcgcttcgat cgcgtcgaag gccttcagcg
     1861 cgtccggcag atcgcgttcg ctagtgcccg acatgcggcc ggtagtcctc gcggcgtctg
     1921 gggatgcgcg gcgccggcgg cggcgccgca ttgtcgagaa aggcgtccgc ccagctatac
     1981 acgtcttcgg tgcggaccgt gcggcgcaac gcgcgcatgc gttgctgccg ttcgccgtaa
     2041 ggcatatgca ctgcctgatg gatggcatcg gccatgccgg cgatgtcgtg cgggttgacc
     2101 agcagcgcgc ccgcctgcag ttgcgccgcc gcgccggcga attcgctcag gatgagcacg
     2161 ccgtcctcct tgatctgcgc ggcgcagtat tccttcgcca ccaggttcat gccgtccttg
     2221 agcgaagtga ccagcgcgat gtcggcggcg aggtagtagg cgatcagatc ctcgaaatcg
     2281 agcgagtgat acatatagtg caccggcacc cagcccgagg cagtgaactc gccgttgatc
     2341 tcgcccacgc actgctccag ttcctgccgc aggcggcggt attcgggaat gtcgctgcgg
     2401 ctgggcacca cgtgctgcac cagcgtcact ttctgatgca gatcggggta gcgctgcagc
     2461 gcattgcgga aagccgtcag cttgttgatc agccccttgg tgtagtcgag ccggtcgacg
     2521 ccgagcatga gctgcgagcg cgccagatcg gtacgcagca ccgcggcctt ggcccggcat
     2581 tcgtcgcgcg cggcggccgc cgcgatcgcg tcgtaatcga tgccgatcgg aaaagcaccg
     2641 acctggacgg tgcgcgcgcg ttgcgtggaa gccggctcgt ccaccggatt gcgcggcatc
     2701 ggcaccacca tggcgtggct gacgggcgac agactgcacg caccttcggc cggcgtgcgc
     2761 gcgtcgccgc gcaggggcac cacggccgcc ttgaattcac ccatcttggg caattcgtcg
     2821 ggggtgacgg cgcggcgcga ttcgacggcc gactcgcacc aggtttcgag cgaatcgtcg
     2881 cgcacacgga cgcgcacgag gcgaccgctg ccctccaggc ggatgtcgaa cagggcatgg
     2941 gcgcaggcga ggaagttgtg ctggtcgcgc gcggtctgga agccgagcgt atcgaaggcg
     3001 agcaggctgc gcaggatttc gaagcgccac ggcagtttga ggaacatctc cagcggcgga
     3061 aaggggatgt gcaggaagaa gccggtccgc gcagcgacac ccagccgctt caactcgcgc
     3121 gcgagttcca tgaggtgata gtcgtgcacc cagatgtaat cgccttcgcg gtgttcctgg
     3181 gccacggccc gggcgaactt gcggttcacg cgccggtagg cctgccagta gtccgggtcg
     3241 aaattgcagt tggtgaacag gtcgtggaac agcggccaga tgatttcgtt ggaaaagccc
     3301 tggtagaagc cttccacttc ctcgggagcg agcatgaccg cccggcagcg atagccggcc
     3361 gacatggcgg ccagcgcccg ctcgatttcc gcctgcggac aatcgctggc gcccggccag
     3421 ccgatccaga ccccgccgca gcgggtcagg acgggttcca gggcgctgac cagaccgccg
     3481 ctgctcggcc gggcctccca gcttccgtcc tccttccggt tgagcgtgac gggcagacgg
     3541 ttggaaacaa tgagcgttct ctcaggcatc ggcttgtcct ctttggtcgg agagccggcc
     3601 cggcgatcat gcgggggcga cggcggggcg cggcgctccg atgctcaaat cggggattcg
     3661 agtcatatag gggctactgc ggcgcatggc aagtcgccgc aggagttttc gtagccccgg
     3721 ttgcatggaa caggcctgtt tgctgtaagt gggtatttac atatctatcg ccatccctat
     3781 gacgggatcc cagccgggtg aggcacgtgg cgaacagggc gccggcgcag ggtgcggcga
     3841 gaacgtatcg gtagaaagcg ccatagtcgc ccccaccggg gctcagcgcc ccgttcgcgc
     3901 cgtattctgt tcagcgcatt tcgaatgctt cctgatgaaa aggcgtcgcg cgcatgccta
     3961 ggcggcgcaa tccgctgcgc aatgcatcgg ccagtccgcg cgggccgcag aaccagattt
     4021 ccgcgggctt cgttcgcgag tggcttgcgt agagtagttc ggccgtcaaa ggcgccccgt
     4081 catcgccgtt gatatgcagc cgaacggagg gaagggtcgc gcatagcgcc ttcaggcgca
     4141 tgacaaacgg gtcgtgatcg ggcgcccgcg tgcaatagta gaggtcgacg cgcggcgcct
     4201 gcgctggagc gccctgcagc gactcgagcc aggcgagaaa gggtgtgacc ccgacgccac
     4261 cggcaatcca gatctgcgcc gcgcctctgc ggctgcggcg aaagtcgaag cggccgtaag
     4321 ggccttcgat gcgcatcgtc cgtccgggat gcagacgcga cccgagtttg ttcgtgtaat
     4381 cgcccagcgc cttgatctgg aatgtcacgc gcctgtcact gctgtcggcg gatgcgatgg
     4441 taaaggggtg atggccttcc aggcggtcga aagtgacgaa tgcgaactga ccggcacgat
     4501 gtccacgcca cttctgatcg agacgacagg tgacttcagt gatacccgcg cccggctcgc
     4561 ggacggattc gatgacgccg ccggcccgcc gtcgtgagcc gatcatgccg ctgagcgatt
     4621 gcacgctcgc agccgaaccg gccgcaatga gtagcgcaag cagtatgccg atcggttgtt
     4681 gccagtagtc gaccggtgcg agaaaggcgg cgtgaacgac gagcataagg tagagcaccg
     4741 gcatcgcctt gtgcagatag cgccagacgc tgaagggaaa gcgcttccac agcgtcagga
     4801 ccaacatggc gagcagggca tagatcgccc attcgccgag atcctcgccg agctcgcgca
     4861 tgatctcgaa gaaaccgccg gggtgttcga tcgatacgcg tcctgcgcgg ccccacagcg
     4921 atttgatgag gtcgtcggac atctcgatga gccaatgcag cgcggcgaat gcgacggcaa
     4981 ggatgccggc ccatttgtgc gtgcgataga tgcggtccat gccgccgagc gggcgttcca
     5041 gccaggtcgg ccgggtggcg agcaccatcg ccagcgaaag caacgcaatg gaccacagac
     5101 ctgtcaggta gagcgcttcc tgacgcacga gccagggcgt tgcggccgca gggtccagcg
     5161 gcgtggcggc ccatgcaagg gtgacgagtg cgatcaggcc tgcaagcaaa ggtttcatat
     5221 cggaatgcct ggctgcggcg ccatatcgac gccgcagcga ctacaactca aatgatgccg
     5281 ggtgcctact gcatcgcccg atcgcgtttg tcgctcttcg tgcgcgtgcc gagcacttcg
     5341 cccgtcactg catcgacctc gagcttcacg cgacggcctt ccgcgtcgac ggctttcact
     5401 tcgaagccgc gatcttcacg ctcgatttcg tcgatgtcgc gataaccctg cgcgatgagt
     5461 ttgtcgtgca cttcgccgat agtcagtcgc ttggacgtcg atgccgtggc ctgcccggca
     5521 tcggcgttct gtgcgatggc gggtgcgagc aatgtgccgc cggcgacaag tccggtgctc
     5581 agtgcgacga cggcgaggaa ggatgtggaa gtacgcatgg tgattctcct gttcggttga
     5641 ggggtcgcgg tgcgaccttg aatccattgt gtgcgcgcgc tgctgaactg atcgtgaagc
     5701 gtgcgttcat gcgcggttca gcaagcggac ggcatcagtc gtcgtcgaag tagcccttgt
     5761 cggcatcgat atgacaggcg ccgcagttgg ctttggagcc gacttccttg ctcgtccact
     5821 cgcgttgcga aacttcgtcg tgctcgtgca gccagcgtgg cagttcggtg atgcgcagcg
     5881 gcgcctcgtt ggcgcggacg tcgcgcaaca gcttgcgtcc gtagcgcgcg ccgccggtgt
     5941 cgccggcgtt ggcgacgaga tagcgcgcga tggccgcagc cgtttccgcg tcgacgctgg
     6001 catcgtcacc gaaatgattc tgcaggtcgg ccatcatgcg tttccaggaa gcggtgggca
     6061 gcatcgacgc cggataggca agatggcaac tgccgcactc ctgcagcgtg agcggatcct
     6121 tcacgggcgg aaagtagtgg tcgctgccgg cctgcgcgcg gctgtacagc aaggcggaga
     6181 acgacagagc gatagcgccg atggcgagtg ctcgaacagg gattctcatg gcggtcatcc
     6241 tctactgctt catcatgtag gcgagccagt cgcctttttc gcgcggcgtg cattcgcggc
     6301 cgagcacctc gttgcagttg cgcttgaacc acttctccac cttcgcggga tcggtgtaac
     6361 gcttgggcga gacggacacg gccatcggtt cgatcgcctt gccggtgcgc gtgcggcctg
     6421 caacccgcgc atctttctcg tggcaggacg tgcaggccag ggtatccggc ttgccgcgcg
     6481 cgaagggagt gcgatggagc gtctctccgc gtgcggccga gaagttgtcg aaagcgggct
     6541 cggcggcttt cgcctcgcgt gcatagcgat cgagcagatc ttcgcgcggg ccggcgctta
     6601 cccccgcggc gaaaccgagc gcgcacaccg caatcattgc gtaagccgac ttcgtcatga
     6661 tttcatccct tcctcaccca agcggtccga tcgaccggta cgcgacgaag tgtggtcgct
     6721 tgttcctgaa cggccgctga aatcggtgtt catgtaccgt tcagcttgcc gctgctagaa
     6781 cgtctttcac gtgtaacgcc ttcatgggat gccgccatgc gtcgcctggc ttggattgtc
     6841 actttgctgc tcgccgtatc ggctgcgcag gcggacgatg cgcgcgatca tgaccgcgcg
     6901 cgcgaggcgc tcgaggcggg cgaggtcatg ccgctgccgc gtatcctcga tcgcgtggaa
     6961 cgcgactacc cggggcgtgt gctggaagtg gagttggagc gcgacgacgg acgctggctt
     7021 tacgagatca agctgttgca gcccgacggg cgagtgctca agctagatgt cgacgccgcc
     7081 gatgcccgcg tgttgaaagt gaaggggcgc gagcaccacg agcgccgcga tgaacggaga
     7141 tcacgctgat gcgcatcctg gtcgtggagg acgagcacgt ccttgccgcg cagctgtgcg
     7201 aagcgctgtc cgaagccggt tatgccgtcg acgtcgcgca taacggtctc gacggttatc
     7261 accttggtgc agaggaaagc gtcgatgcgg tggtgctcga cctcgggctg ccgcatatgg
     7321 acggcatcac catgctgacg aaatggcgcg cggccggccg cgacatgccc gtgctgattc
     7381 tcaccgcgcg cgacgactgg catcagaagg tagccggtat cgatgcgggc gcggatgact
     7441 acgtcaccaa accctttcac atggaagaac tgctggcacg tgtgcgcgcg cttattcgcc
     7501 gggcgggcgg ccacgcgagc gcgacgctcc actgcggacc gctgacgctc gatacccggg
     7561 ccggacgtct gaccatcgaa gggcgcgcgg tgccgctcac cagccacgag taccgtgtcc
     7621 tggcgtatct catgcaccac acgggcgaag tcgtctcgcg tactgaactg accgaacaca
     7681 tctacgcgca ggactacgac cgcgactcca acaccataga agtgttcatc ggccgcctgc
     7741 gcaagaaact gccgccgggc atgatcgaaa ccgtgcgcgg actcggttat cgactggtcg
     7801 acggcaaatg aatctgcgcg actccttgcg cttgcggctg ctcgccggga gccttgtttg
     7861 gatcgtcgtg acactcacgg tgaccggctt tgtgctaacg gacctgttcc ggcaacacgt
     7921 ggcggcacgc ttcgaagcgg agttgcaagg ccatctcgac caactcgcgg cggtgttcga
     7981 attcgccgag gacggtatgc cgcgtctccg cgcagagttg agcgacccgc gtctccgcct
     8041 gccctattcc ggcctgtact ggcagatcga ccgcgccgca aatggcgcgg cggtcggcgt
     8101 gctgcgttcg cggtcattat gggacagcac ccttacagtt cccgccgacg cgctcgccga
     8161 cggtcaggcc catgtgcatc gcgtcgatgg tcccgacgcc gcgcaactga tcatggtgga
     8221 gcgcatgatc cggccggcca gccggccgga aaccgcttat aggttgatcg tcgccgccga
     8281 cgagcgggcg atgactgagc cggtcgccgc ctttgtcggc ctgctggtgc gtgcgctggc
     8341 gattctcgct gcgggattgg tcgcgctggt agcgctgcag ttgtatctgg ggctgatgcc
     8401 gttacggcgc atgcgcgagg cgcttgcgac cgtgcgggcc ggccggtcgc gtcaactggc
     8461 cggccgatat ccgcaggaag ttcagccgct cgtggatgaa ctcaacacgc ttttacgtca
     8521 cgatgccgaa gtcgtagcgc gtgctcgaac gcaggcggga aatctcgccc acgcggtgaa
     8581 aacgccgctt gcgattctgg ccaacgccgc ctcacgcgag cagggcgagt tcagtcgcct
     8641 ggtcgctgaa caggcagcga tcgcgcgccg gcaagtggac tatcacctcg cccgtgcccg
     8701 ggctgcggcg gcgacgctgg tgcccggcat gcacacgccg ctgcgacccg cgctggaagg
     8761 actagtgcgc gtgatgcggc gcctgcatgc ggagcgtgag ctcgaaatcg atgtcgcgca
     8821 ggtgcccgac aagctcggct tcgcagggga agagcaggat ctgcacgaaa tgctgggcaa
     8881 tctgctcgat aacgcctgca aatgggcgag gcagcgcgtc gtcgtcaatg cgtcactcga
     8941 aggtcggacg ctcgcggtcc gtatcgatga cgatggcccc gggctcgcgc ccgccgaacg
     9001 cgaggcagtg ttcgcgcgcg gcgcccgggc cgacgaacaa gtgccgggga gcggcctggg
     9061 gctcgcaatc gtccgggatc tggcgcatct ctacggcggc gatgtgcacc tcgacgtcgc
     9121 gccgcaaggc ggactccgtg tgacgctcac actccctgcg gcttgaaggg gctttatccg
     9181 cccgttattc aatctggatc aatcccgccc caggtgttcc gtgaaaacat gcttcagcgc
     9241 gggccctcgc ctgagcattg tcatgaccga aggagtcgtc gagtgagcga aacagtcacc
     9301 gttaccatca cccagttgga taactaccgc ttcaacgtgg atttcggcgc cggcctgccc
     9361 caactcgttg ccgacgaacc cgcgccgatg ggggccggca cagggcccga tccggcgcga
     9421 ctgctgctgt cggcggtggc caactgtctg tcggccagcc tggtgttcgc gttttccaag
     9481 ttcaagcagg acccgggcgg tatcacgacg actgcaaccg cccacatcga gcgcaacgaa
     9541 cgcaaccgcc tgcgggtcgg ccgcatcgac gtgactatcc gtttgggccg tgccggcggg
     9601 gaactggagc atctggagcg gctgctgggg cagttcgagg acttctgcac cgtttcgcaa
     9661 agcgtgcagt ccggtgtgcc gatcgccgtg caggtggagg atacggccgg cacggtcttg
     9721 aaacccggcg gggtgacggt ctgaaacgcg cacgagacgt ttttatagag agagttccat
     9781 gttgataccc aagaccctta ccgttgcgat cgtggcggcc attctttttg gaacgagcgt
     9841 gggcgtgctg gccgcctcgg atcatgccca tcacggcgga ggggttgcgc acctgcagtt
     9901 gaacgaaggc cgcaaatggc cgaccgatgc gccgttgcgc cagggcatgg gcaatatgca
     9961 ggcggcgatg agccaggcat taccgcagat ccaccacggc aagctggacg cggccggtta
    10021 cgccgcgctg gcggcgcggc tcgagcgcga aatggcgtat gtggtggcga actgcaagct
    10081 cgaccccgac gccgacgcgc agcttcatct ggtgctggcc cagttgggcg caggcctgga
    10141 acaaatgaag gcgggcggcg atcggcgcgc cggcgcagtg tccgtcgtgg acgccttgga
    10201 tgcttatggc acccacttcg accaccccgg ttggcacgct ctgcggcatt aggccgcccg
    10261 tacgcatttg ggcgtttccg gaacggtcaa acgaccccat cgcgggtaga atgcggctgt
    10321 tccggaaccg atgcgcgctt ggcgagtccc ttgtacagac tgcccgggca tcgggcagca
    10381 gacataacga ttccgcatga aaccccgtca taaacgcatt gccctcatcg ccggtgggct
    10441 ggctgcgctc ggctttgccg ccgcgctggt gctgaacgcc tttcagagca atctggtgtt
    10501 cttcttcagc cccagccagg tggtggccaa ggaagcgccg ctggacaaga ccttccgcat
    10561 cggcggcatg gtggaggacg gcagtctcaa acgtctgcct gacggcttga gcgtggaatt
    10621 caaggtgacg gacaccgcgc atgccattcc ggtgacctac acgggcatcc ttccggatct
    10681 gttcaaggaa gggaagggcg tggtcgcgca gggccggctc ggaccggacg gcatgttccg
    10741 cgcgaccgaa gtgctggcca agcacgacga gaactacatg ccgcctgacg ctgcgcacgc
    10801 cattgaacag gcgcagaagg cgcagcagac gctacagcaa tgattccgga actcggccat
    10861 ttcgcgttga tcgtcgcgct catggtggcg ctggcgcagg gcgtgcttcc catcgtcggc
    10921 gccgcgcgcg gcacggtttc ctggatggag atggcgcggc cggccgcgca ggtgcagttc
    10981 gcgctggtgg ccttcgcctt cttctgtctg gcctggtcgt tcgcgcacaa cgacttctcg
    11041 gtgctcaacg tcgcatcgaa ctccaattcg caactgccgc tgcactaccg cgtcgcggcg
    11101 gcctggggtt cgcacgaggg ctcgctgctg ctgtgggtct tcatgctcgg gatgtggacc
    11161 tgcgcggtgt cgttgttctc gcgacatctg ccgctggaga tggtgtcgcg cgtcatcggt
    11221 gtgatgggcc tggtcagcgt cggcttcctg ctgttcatgc tgctcacttc caatcccttc
    11281 gagcggttgc tgcccgccgc gcccgatggg cgcgatctca acccgctgct gcaggatccg
    11341 ggtatggtca tccacccgcc cttgctctac atgggttacg tcggcttttc ggtcgccttc
    11401 gcgttcgcca tcgccgcttt gctgagcggc aagctcgacg ccacctgggc gcgctggtcg
    11461 cggccgtgga cgacgctggc ctggtgcttt ctgaccctcg gcatcgccgt cggcagttgg
    11521 tgggcgtatt acgaactggg ttggggcggt tggtggttct gggatccggt ggaaaacgcc
    11581 tcgttcatgc cctggttggt cggcaccgca ctgatccatt cgctcgccgt gacggaaaag
    11641 cgcggcagct tcaaaagctg gacggtgctg ctcgccatcc tcaccttctc gctgagtctg
    11701 ctcggcacct tcctcgtgcg ctcgggcgtg ctgacctcgg tgcatgcctt cgcgaccgac
    11761 ccggcgcgcg gcgtgttcat cctcgccttc ctggtccttg tcatcggttc ctcgctcgcg
    11821 ttgttcgcat ggcgctcgcc caaagtgggc ctgggcggca agttcgacat ggtctcgcgt
    11881 gaatcgacgc tgctgatgaa caacgtgctg ctggtcgtcg cggccggcac ggtgctgctc
    11941 ggtacgcttt atccgctgtt cctcgacgcg ctgaacctgg gcaagatttc cgtcggcccg
    12001 ccttatttcg aagcggtgtt cgtgcccttg atgacgccgg tggtgtttct catgggcatc
    12061 ggtccgctga cgcgctggaa gaaggcggag attcccgatc tggcgaagcg gctgcgctgg
    12121 gccgcgggcg tcagcctcgt caccgcgctg cttgcgccgc tggcgggcgg cgactggacg
    12181 gcgatgtccg ctttcggcta tctgctcgcc ttctgggtcg tcgccacctg tgtcgtcagc
    12241 ctgaaggaac gcatccgcaa tttccgcggc ggactgtggg cgcgtctggc cgcgcagccg
    12301 cgttcgtact acggcatgct gctcgcgcat ctgggcatcg gcgtgttcat cgtcggcgtg
    12361 acttcggtga tgagcttcga agtcgagcgc gatgtgcgca tggacgtggg cgacagcgtg
    12421 gtcgtcgggc cgcacagctt ccgtttcgac ggcgtgcgcg aactgcaggg tcccaactac
    12481 accggcgcgg aaggcatcgt caccgtgagc cgcaacggca agacggtcgc cgtgctgcat
    12541 ccgcagaagc gtctttacac cgtgcagcag atgccgatga ccgaagcggc catcgactcc
    12601 ggccttcttg gcgacattta tgtatcgctc ggtgaacccg tgggcgacgg cgcatgggcg
    12661 gtgcgcgttt atcgcaagcc cttcgtcacc tggatctggg gcggctgcgt gctgatggcg
    12721 ctgggcggct tcattgcgct cgccgaccgg cgctaccgcg tgcgcgtgcg tcgcaccgaa
    12781 cccgccgcgg ccgtcggcga agcggcatag gaaggcgcat gtccaagtat ctgctgccct
    12841 tgggcgtgtt catcaccgtt gccgtattcc tgttctacgg cctgagcctc aatccgcgtg
    12901 aggtgcccag tccgctggtg ggtaagccgg cgccggcgtt ccagttgccc gtactgcaca
    12961 gccccgataa gacgttcacg ccgaaggaaa tggcgggcaa ggtgtggctg ctcaacgtat
    13021 gggcgtcatg gtgtgtcgcc tgtcgggacg agcatccgct gatggtggaa ctttcccgtc
    13081 gcaagatcgt tccggtggtg ggcctgaact acaaggatca gccggacgac gccaagaaat
    13141 ggctgcggca tttcggcgat ccctatctca cttccgtgtc cgacctcgaa ggccgcgtgg
    13201 gcatcgactt cggcgtttac ggtgtgcccg agaccttcgt gatcgacaag gacggcatca
    13261 tccgtcacaa gcacatcggc ccgatcaccg aagac
//
LOCUS       DATGOU010000127        33307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_835553, whole genome shotgun sequence.
ACCESSION   DATGOU010000127 DATGOU010000000
VERSION     DATGOU010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 33307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33307
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835553"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VKZ48_07400"
     CDS             complement(<1..557)
                     /locus_tag="VKZ48_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HLU77710.1"
                     /translation="MSAGLLFALVCAVLALVYGIISISWILKQPSGNERMREIAGAIQ
                     QGASAYLNRQYTAIGIVGVVLLIVIWLALGLSTAVGFAIGAALSGAAGYIGMHVSVRA
                     NVRTAEAARKGLNEALSVAFRGGAITGLLVVGLGLLGVAGFYAVLAAMGGPETSQADL
                     IKPLVGLGFGGSLISIFARLGGGIFT"
     gene            complement(845..2098)
                     /locus_tag="VKZ48_07405"
     CDS             complement(845..2098)
                     /locus_tag="VKZ48_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HLU77711.1"
                     /translation="MARPKNALYAQSGGVTAVINATACGVIETARAAKGRIGKLYAAR
                     NGIVGALNEDLIDTSRESARAVSALKHTPGGAFGSARFKLKSLEEDRSQYERLIEVFR
                     AHDIGTFFYNGGNDSMDTAWKLSRIGEELGYPIACIGVPKTVDNDLALTDNCPGFGSV
                     AKYVATSMREAGFDVASMHSTSTQVFVLEVMGRHAGWITAACGLAAESSDEAPQILLF
                     PEITFDKAKFLARVERTVKRVGWCAIAVSEGLKDADGRFLAEAGTRDAFGHVQLGGVG
                     PRIAQMITDELGYKNHWAVADYLQRAARHLASKVDVEQSYALGVAAVKLALAGQNAVM
                     PTIVRVSDKPYRWKIGTAPLKDVANKEKKMPRSYISRDGFHITDACRRYLAPLIRGEA
                     PPPFRNGLPDYVRLKNAPVPKKLGR"
     gene            complement(2247..2900)
                     /gene="adk"
                     /locus_tag="VKZ48_07410"
     CDS             complement(2247..2900)
                     /gene="adk"
                     /locus_tag="VKZ48_07410"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008252374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HLU77712.1"
                     /translation="MRLILLGPPGAGKGTQAAFIKERFNIPQISTGDMLRAAVKAGSP
                     LGLAAKKVMDAGQLVSDDIIIGLVKDRLQEPDCHNGYLFDGFPRTIPQAEALKEANVA
                     LDFVLEIDVDDASIIERMIGRRVHPASGRTYHVKFNPPKVEGKDDVTGEDLIQRDDDK
                     EETVRKRLEVYREQTRPLVDYYSKWAASGDPKAPRYRRIEGVGSVEAIREAVFAALR"
     gene            complement(2937..3710)
                     /gene="kdsB"
                     /locus_tag="VKZ48_07415"
     CDS             complement(2937..3710)
                     /gene="kdsB"
                     /locus_tag="VKZ48_07415"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006762073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HLU77713.1"
                     /translation="MARVPFTVVIPARHASTRLPAKALADIAGKPMVVRVAEQARKSG
                     AAAVLVATDHEAIAEAVRAHGMEVLLTRADHPSGTDRIAEVVDAIGLADEAIVVNVQG
                     DEPLIPPALIERLAEALAARPDADMASVCHAIEDEETFFNPNVVKVVCDRQGDAMYFS
                     RAPIPWARDAFVGNAPGLPAQFPAYRHLGMYAYRAAFLRAYTQLAPAPLERFESLEQL
                     RALWHGHRIAMVVTEEAPPAGVDTPEDLARVRARFAPAS"
     gene            complement(3718..3900)
                     /locus_tag="VKZ48_07420"
     CDS             complement(3718..3900)
                     /locus_tag="VKZ48_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006384001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HLU77714.1"
                     /translation="MDPRLLDILVCPLCKGPLLYRKAEGEFICKADRLAFPIRDGIPV
                     MLEDEARKLDPAEDIA"
     gene            complement(3881..4867)
                     /gene="lpxK"
                     /locus_tag="VKZ48_07425"
     CDS             complement(3881..4867)
                     /gene="lpxK"
                     /locus_tag="VKZ48_07425"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HLU77715.1"
                     /translation="MMRGASGLAQRVWYGRSALSLALLPFSFLFAAVAAARRCAYRRG
                     WLRSERLSVPVVIVGNLVAGGAGKTPLVLWLVEQLRARGWRPGILSRGYQGQNAAPSA
                     VTPGADPAQVGDEPALLAARAGVPVWIGRRRVDAGRVLLAAHPDCNVLVCDDGLQHYA
                     LARDVEIAVEDERGHGNGRLLPAGPLREPASRRVDATVVNGGSRPGAYAMRLESAGFY
                     RVHDGAAVPLEALRGMRLHAVAGIGNPERFFRQLAEMGLRVESHAFPDHHRYADADLR
                     YPDCDAVLMTEKDAVKCRGFGRTDLVALRVEAVVDPALADLIETRLHGSAPA"
     gene            complement(4877..5293)
                     /locus_tag="VKZ48_07430"
     CDS             complement(4877..5293)
                     /locus_tag="VKZ48_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HLU77716.1"
                     /translation="MKFRSRWKEDPEINLIPLIDVLLVILIFLMVTTTYSKYSELQIN
                     LPTANAEKQVERPNELNITVDALGAYTINGRATPFNNVPSLSDAMRRAAGDLENPVVI
                     ISADAKASHQSVVHVMEAARMAGYTHVTFATQSTEQ"
     gene            complement(5382..5990)
                     /locus_tag="VKZ48_07435"
     CDS             complement(5382..5990)
                     /locus_tag="VKZ48_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HLU77717.1"
                     /translation="MFEIIEAAGWPIWFLLIASVIAVALIIERLIALRAPKIIPHNLL
                     EKVVEAYRKQGVTQQLVDAVYADSPLGRVLAAGLRNHGAPRPVIKEAIEEEGRAVTHE
                     LERFLTTLGTIASISPLWGLFGTIVGMIEIFGSQNPSGANPQQIAHGISIALYNAGFG
                     ILIAVPATIFFRHFRGKVEDFVVTMEQQASRLVDMLHGTRKD"
     gene            6452..7810
                     /gene="xseA"
                     /locus_tag="VKZ48_07440"
     CDS             6452..7810
                     /gene="xseA"
                     /locus_tag="VKZ48_07440"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HLU77718.1"
                     /translation="MPELPLTPSGRPTAETILSVSALNRSVRDLLEHRFPLAWVAGEI
                     SNLTLARSGHWYFSLKDDNAQVRCVMFRHRAQFLNWQPREGMHVEARALVTLYEARGD
                     FQLNIEFMRQAGQGALYEAFLRLRDKLAAEGLFDPALKRSLPPFPRAIGIVTSPHAAA
                     LRDVLTTLARRNPSIPVILYPTSVQGEAAPAEIVAALRAASRRAEVDALILCRGGGSI
                     EDLWAFNDEAVARAIRECVVPVVCGVGHETDVTIADYAADQRAPTPTAAAELLSPDRA
                     SLLERIAQLRGTLQRCMTRLLESRMQHVDHLSRRLVHPGERLRAQQRLLTQLCSRLSH
                     AATRDLERRHWQVHSLLERGRRALPNVDARRARLSELMHRLAAASTRGLTAHDARLTH
                     LSRALAHLDPRAVLERGYSLVTDETGRVVRDAHALERGDGLHIAFARGSALARVEDTR
                     ED"
     gene            7904..8482
                     /gene="sodB"
                     /locus_tag="VKZ48_07445"
     CDS             7904..8482
                     /gene="sodB"
                     /locus_tag="VKZ48_07445"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HLU77719.1"
                     /translation="MEHKLPPLPYAMDALQPHMSKETLEFHYGKHHQAYVTNLNNLIK
                     GTEFENASLEDIIKKSSGGLFNNAAQVWNHTFFWNCMSPNGGGAPTGAVADAINKKWG
                     SFDAFKEAFNKSAVGNFGSGWTWLVKKADGSVDIVNTSNAATPLTTDDKPLLTCDVWE
                     HAYYIDYRNRRPDFVGAFWNLVNWEFVNKNFA"
     gene            complement(8528..8965)
                     /locus_tag="VKZ48_07450"
     CDS             complement(8528..8965)
                     /locus_tag="VKZ48_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008644952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HLU77720.1"
                     /translation="MLRGLLIAGALLYAASVMATEMPVVDLNAGIHRIQAEVAHTHQA
                     RTKGLMNRRSMEDNHGMVFSWPEAGRHCMWMRNTHLPLSVAFIDDDGRIVNIADMTPR
                     TDDIHCAAKPVRYALEVNRGWFAQRRLAPGARIGGLDKLPAAE"
     gene            complement(8997..9644)
                     /locus_tag="VKZ48_07455"
     CDS             complement(8997..9644)
                     /locus_tag="VKZ48_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HLU77721.1"
                     /translation="MRSTKALSGPRAKRAILAEPAGRTKRPHGASRVLLFNKPYQVLC
                     QFTREGERECLADYIDVPAVYPAGRLDADSEGLLVLTDDGRMQQRIADPRHKLGKIYW
                     AQVEGVPEAAQLAQLREGVDLGDYVTRPAAARAIAEPAGLWPRHPPIRFRQAIPTTWL
                     ELVLFEGRNRQVRRMTARVGLPTLRLVRAAVGPWSVEGLQPGQWRWVEVRAGDLR"
     gene            9683..10900
                     /locus_tag="VKZ48_07460"
     CDS             9683..10900
                     /locus_tag="VKZ48_07460"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HLU77722.1"
                     /translation="MAKIKVQNPIVEMDGDEMTRVIWQRIRDQLILPYLDIELKYFDL
                     GIESRDRTNDQITIDAANATRQYGVAVKCATITPDEARVKEFNLKQMWKSPNGTIRNI
                     LDGTIFREPIICRNVPRLVSHWDKPVVVARHGFGDQYRATEHKFEGAGTLKIVFEPAD
                     GGAPVEKEVFKAPGAGIAMAMYNLDDSIRGFARACFNYARQRNYPVYLSTKNTILKTY
                     DGRFKNIFQEVYEAEFKQAFEGAGLSYEHRLIDDMVASNLKWNGGYLWACKNYDGDVQ
                     SDTVAQGYGSLGLMTSVLMSPDGKTVEAEAAHGTVTRHYRQHQQGKPTSTNPIASIFA
                     WTRGLQHRGRMDSTPEVVKFAETLEQVCIDVVESGRMTKDLAILIRPDHPFLTTDQYM
                     DAVNEELKRRMAA"
     gene            complement(11029..11235)
                     /locus_tag="VKZ48_07465"
     CDS             complement(11029..11235)
                     /locus_tag="VKZ48_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HLU77723.1"
                     /translation="MPTGTVKWFNDAKGYGFITPDDGGEDLFAHFSAIQMNGFKTLKE
                     GQKVQFEVTQGPKGKQASNIQSAS"
     gene            11535..11843
                     /gene="clpS"
                     /locus_tag="VKZ48_07470"
     CDS             11535..11843
                     /gene="clpS"
                     /locus_tag="VKZ48_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HLU77724.1"
                     /translation="MATRSGEGTILEPSKSKVAPPPLFKVILLNDDYTPMEFVVVVLQ
                     TFFSMDRERATQVMLAVHREGMGVCGIYPRDIATTKVEQVTAYAREHQHPLQCVMEET
                     "
     gene            11844..14117
                     /gene="clpA"
                     /locus_tag="VKZ48_07475"
     CDS             11844..14117
                     /gene="clpA"
                     /locus_tag="VKZ48_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HLU77725.1"
                     /translation="MIAQELEVSLHMAFVEARQKRHEFITVEHLLLALLDNPTAAEVL
                     RACAANLDELRKALTEFIGAHTPTVAGSEEIDTQPTLGFQRVIQRAILHVQSSGKKEV
                     TGANVLVAIFGEKDSHAVYFLHQQGVTRLDVVNYISHGITKSGQPSQPKATEEAEQQE
                     EGQQSGGALETYTVNLNAQALVGKIDPLIGRDREVERVIQTLCRRRKNNPLLVGEAGV
                     GKTAIAEGLARRIVEGQVPDILAQSTVYSLDMGALLAGTKYRGDFEQRLKAVLKQLLD
                     NPNAILFIDEIHTLIGAGAASGGTLDASNLLKPALSTGQLKCIGATTYQEYRGVFEKD
                     HALSRRFQKIDVNEPSIEETVEILKGLKSRFEAHHGVKYSANALSTAAELSARFINDR
                     HLPDKAIDVIDEAGAAQRVLPKSKQKRVIGKPEIEEIIAKIARIPPQTVSSDDRAALK
                     NLDRDLKAVVFGQDKAIDALVTAIRTARSGLGSPNKPIGSFLFSGPTGVGKTEVARQL
                     AYCLGVELVRFDMSEYMERHAVSRLIGAPPGYVGFDQGGLLTEAITKKPYCVLLLDEI
                     EKAHPDIYNILLQVMDHGTLTDNNGRKADFRNVTIIMTTNAGAEALTKTTMGFAAPTA
                     EGDEMADIKRLFTPEFRNRLDGIISFRQLDHDIILRVVDKFLMQLEEQLHEKKVEAIF
                     TEKLKDYLAKRGFDPLMGARPMARLIQDTIRKALADELLFGKLANGGKVTVDLDDDDE
                     VVLRFVEEEEETATPSA"
     gene            14244..14567
                     /locus_tag="VKZ48_07480"
     CDS             14244..14567
                     /locus_tag="VKZ48_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLU77726.1"
                     /translation="MNGRLNLGLRLCALLAFAPAAALAQNAESNTARDLAAACAICHG
                     TNGFNAGGLPHLAGQPKDYIVQQLKDFRDGKRPATIMHQVAKGYTEEQYELLGAWFSA
                     QQRKK"
     gene            14643..15872
                     /locus_tag="VKZ48_07485"
     CDS             14643..15872
                     /locus_tag="VKZ48_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FCSD flavin-binding domain-containing protein"
                     /protein_id="HLU77727.1"
                     /translation="MVSGCASVGGKSSVKSAGRVVVVGGGYGGATAAKYIRMWSGGAI
                     EVTLIEPNTQFISCPLSNLVLGGARTLDSLTMGYDTLRAHGVKVVHDVVTTVQADKKR
                     VTLGKGGELAYDRLIVSPGVDFMYDKLPGLNNAAAQQQVLHAWKAGPQTLALRKQLEA
                     MPNGGVYVLSIPVAPYRCPPGPYERVCQVANYFKQAKPRSKVLVLDGNPDIVSKKGLF
                     LAAWKELYPGLIEYVPNEVARDVDVKTRTIKTEFESFKGDVLNVLPPMRAGNIAASSG
                     LITTNDRWCDVNWLTMESVAVPNVHVLGDSTLSAPAMPKSGSMANNHAKIAASAIVAL
                     LTGQTPNPTPIIGNTCYSYVSDRLAMHVNSVHRYDAEKKTLLPVAGSGGVSAKHSELE
                     KSYADAWARNIWADMLV"
     gene            complement(15941..16390)
                     /gene="dut"
                     /locus_tag="VKZ48_07490"
     CDS             complement(15941..16390)
                     /gene="dut"
                     /locus_tag="VKZ48_07490"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HLU77728.1"
                     /translation="MHRIDVKILDPRLHDQPPQYATEGAAGLDLRACIDGPLDLLPGA
                     TALVPSGIAIHLADKGLAAMVLPRSGLGHKHGIVLGNLVGLIDSDYQGQVMVSLWNRS
                     NTPFTLQPMERIAQLVIVPVLQVALNVVEAFEESERGAGGFGSTGTA"
     gene            complement(16408..17598)
                     /gene="coaBC"
                     /locus_tag="VKZ48_07495"
     CDS             complement(16408..17598)
                     /gene="coaBC"
                     /locus_tag="VKZ48_07495"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HLU77729.1"
                     /translation="MSELTGKRILLGLTGGVAAYKAAELCRLFVKAGADVQVVMTESA
                     CRFVAPLTLQALSGKPVYLDMWDARIANNMAHIELSRDRDLILVAPATANFMAKLAHG
                     FADDLLSTLCLARETPLYVAPAMNRQMWGNAATQRNAAQLRADGVYLLGPDAGEQACG
                     ETGMGRMLEPEQLYAHIATALGDKPLAGKRVLLTAGPTFEPIDTVRGITNLSSGKMGF
                     AVAQAAVEAGAQVTLVAGPTSLATPTGAQRVNVTTAREMHRAVMDRVTEADVFIGVAA
                     VADYHVANASTQKLKKSQCKPQIELSENPDILAEVAALADAPYCVGFAAETENLREYA
                     QAKRLRKSIPLLAANRADRAFGQDENELILFHEGGETRVPRASKLEVARGLIREIAAR
                     LPNS"
     gene            17714..18388
                     /gene="radC"
                     /locus_tag="VKZ48_07500"
     CDS             17714..18388
                     /gene="radC"
                     /locus_tag="VKZ48_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HLU77730.1"
                     /translation="MGIADWPEGERPREKLLERGAAALSDAELLAIFLRVGTRGRSAV
                     DLGRELIAHFGSLSALLHHAPGETAVINGIGPAKWAQLKAALELGRRALAETARETDA
                     LSSPAAVRDYLRLLLAERPHEIFVAVFLDAQNRVIASEELFRGTLTQTSVYPREVVKA
                     ALRHNAAGVILAHNHPSGIAEPSQSDQLLTDTLKRALAMIDVRVLDHFIVGRGAACSF
                     AERGML"
     gene            18452..18685
                     /gene="rpmB"
                     /locus_tag="VKZ48_07505"
     CDS             18452..18685
                     /gene="rpmB"
                     /locus_tag="VKZ48_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HLU77731.1"
                     /translation="MARVCQVTGKKPMVGNHVSHANNKTKRRFLPNLQYRRFWVESEN
                     RWVRLRVSNAGLRLIDKNGIESVLADLRARGEI"
     gene            18699..18854
                     /gene="rpmG"
                     /locus_tag="VKZ48_07510"
     CDS             18699..18854
                     /gene="rpmG"
                     /locus_tag="VKZ48_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013147069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HLU77732.1"
                     /translation="MREKIKLESTAGTGHFYTTTKNKRTTPDKIEIKKYDPKARKHVL
                     YKETKLK"
     gene            complement(18906..19160)
                     /locus_tag="VKZ48_07515"
     CDS             complement(18906..19160)
                     /locus_tag="VKZ48_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77733.1"
                     /translation="MADKDPIQGEGDYTAAKHYNERTKQFVQSGKVEQAADEAAPQSQ
                     QEAQEMREAERAGKRRCKGEDPALYDPSKIPEDPEFPRDE"
     gene            complement(19270..20430)
                     /locus_tag="VKZ48_07520"
     CDS             complement(19270..20430)
                     /locus_tag="VKZ48_07520"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HLU77734.1"
                     /translation="MLYSGVFDLPWWGVMIVALVLTHITIASVTIFLHRCQAHRALEL
                     HPIASHFFRFWLWLTTGMVTKEWAAIHRKHHAKCETPEDPHSPQQVGINRVLWGGVFL
                     YVKESYNKETMERYGHGTPDDWLERKVYSRYVKTGLTIMALIDLALFGLIPGALVFLT
                     QIVWIPFWAAGVINGLGHYWGYRNWEAEDASTNIFPWGILIGGEELHNNHHAFASSAK
                     LSSKWYEFDIGWMYIRIMEMMGLAKVKKVAPRPKFAPAKSTVDLETLQAVLTHRYDVW
                     AKYAKSLKTTFAQEMSKLAHEPANWRVKRWLVRDESKLCEQQRAKVQEVLAKSEALKT
                     VYSMRRELAALWERSTASREQLLKQLQDWCQRAEASGIAPLREFSMRLRSYA"
     gene            complement(20585..21904)
                     /locus_tag="VKZ48_07525"
     CDS             complement(20585..21904)
                     /locus_tag="VKZ48_07525"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HLU77735.1"
                     /translation="MAVRITPALLETLADLTARALRFERAADKVLSDYFRAHPKLGQG
                     ERGFLAETVYAGLRRKRLVDYILAEVGPVKPQARLTPVATARAFALITLARVRGVNLR
                     ELGLSERSEEGAWLQRVKAAARGELPFEVRCDLPDWVIARLRAHLPDERILAYAEGLQ
                     QPAPLDLRVNLLKSTRDAVQRQLAAEGLRVAPTPYAPTGLRLLDKPALQRHALFLDGT
                     IEVQDEGSQLLCHLLGARRGEMVADFCAGAGGKTLALAALMRSTGRLYAFDVAEKRLA
                     RLKPRLARSGASNVDARHITSEHDARLGRLAGKLDRVLVDAPCSGLGTLRRNPDLKWR
                     QDERALAELRIKQASILAAAARLVKAGGRLVYATCSFLPEENEEIVADFLATHPSFEL
                     VRASVLLESQLEEVPAQPDADGFLRLVPNLHGTDGFYAAALVRKSAA"
     gene            complement(21908..22528)
                     /gene="purN"
                     /locus_tag="VKZ48_07530"
     CDS             complement(21908..22528)
                     /gene="purN"
                     /locus_tag="VKZ48_07530"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HLU77736.1"
                     /translation="MQKKIVVLISGRGSNMQAVVEANLPVVAVISNRADAAGLAYARS
                     RGIPTAVVAHKDYPTREAFDAQLAAEIDRHAPDLVVLAGFMRILTDAFVERYAGRMIN
                     IHPALLPAFTGLDTHARALAAGVKLHGCTVHWVTPTLDEGPIIIQAAVPVRPDDTPER
                     LAARVLRQEHVIYPRAIRWFLEDKLRVENGVVRVAGNEPQLVYSDA"
     gene            complement(22616..23209)
                     /locus_tag="VKZ48_07535"
     CDS             complement(22616..23209)
                     /locus_tag="VKZ48_07535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HLU77737.1"
                     /translation="MQNSRILAFAGSGSRPSLNRRLLEVAVRAAEAAGAQVTVLDLAD
                     YPLPLYTGELEAREGLPANARKLKAIFVAHAGLLIASPEHNASVSALLKNTLDWVSRP
                     DSGQNGLVPYQNKVAALLAASPGNIGGLRGLTQLRAILQSLGVLVQTKQYALARAHEA
                     FDADGALLDPRVHASVEGVARQLVRMVRAFEAAREDA"
     gene            23334..24248
                     /locus_tag="VKZ48_07540"
     CDS             23334..24248
                     /locus_tag="VKZ48_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLU77738.1"
                     /translation="MNRMKLSLEALQVLDAIDRKGSFAGAAAELHRVPSALTYQVQKL
                     EQDLDVLLFDRRGHRAVLTAPGRELLKEGRNLLRAAGDLESRVKRLATGWEVELRLTL
                     DTSLSLQGLLDLVAAFYREDAGTRLRIGHEVLGGTWDALVDRRADLVIGASGEAPTGG
                     GYSSQPLGEVEWLFAVAPHHPLAREAEPLERDTILRHRAVAVGDTSRRLPPRSTGLYN
                     GQDVLTVPDMQAKLQAQLTGLGCGFLPRLLAQPLIDAGRLVAKEVEESKPSGMLRYAW
                     RTGETGKALKWFLKRLEQAETRRALLGC"
     gene            24242..25153
                     /gene="gluQRS"
                     /locus_tag="VKZ48_07545"
     CDS             24242..25153
                     /gene="gluQRS"
                     /locus_tag="VKZ48_07545"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HLU77739.1"
                     /translation="MLNTDVYRGRFAPSPTGPLHFGSLVAAVGSFVDARAHGGTWLVR
                     MEDLDPPRVVRGAADNILRTLEAHALHWDGDIVYQSTRHDAYSAALEQLAAKGATFSC
                     ACTRKEIADSALGPDGAAVYPGTCRNGLPPGRSGRAVRVRVDDTPIVFHDRLQGNQRH
                     DLAHEVGDFIVRRADGVHAYQLAVAVDDAYQGITDIVRGADLLASTPRQIHLQRLLGF
                     ATPRYLHLPVATNARGEKLSKQTLAAPVDAEYASAGLIAALGFLDHRPPVALHGAPPE
                     ELLQWAVANWQVARLPRTRQLPHPVSA"
     gene            complement(25160..25930)
                     /locus_tag="VKZ48_07550"
     CDS             complement(25160..25930)
                     /locus_tag="VKZ48_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HLU77740.1"
                     /translation="MKFSALIIGDEILRGKRTDRHFAKMVELLSARGLYLSRAEYVGD
                     DRTGLVEVLRRSFAQGGAVFCFGGIGSTPDDNTRQAAAEALGLKLVRHADAIALIEAA
                     MQRRYGRAASPQQLQMGEFPEGAQLLPNSYNTIPGFSIRDHHFMPGFPVMAHPMAEWV
                     LDHHYAHLHHSLSERELSVYVYGIPESAATPLMIEAESRYPGVRVFSLPYVGKDVRRH
                     IELGVRGEPAQAEAAMKLLREGVLALGATCEDEAPTRT"
     gene            complement(25927..26646)
                     /locus_tag="VKZ48_07555"
     CDS             complement(25927..26646)
                     /locus_tag="VKZ48_07555"
                     /inference="COORDINATES: protein motif:HMM:NF018917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HLU77741.1"
                     /translation="MTEIAKALVRALASTLHPRMILLMIWPLALALLFWGVIALLFGA
                     QTVAWLEGQIATSAVAQWVSQWVPFEPVAAVLGWAALLILFVPLVIVTASFIVGVFGM
                     TIMVEHVARNYYPELRREQGGSIAGMTVNAFVALGVFVLLSLVTLPLWFIPLLWPVLP
                     VLLLGYFNQRMFRYDAVSEHATPEEMRLLFKRHNAAMYGLAIILALIAQIPLVGFFIP
                     VIAGLAFIHYGLAQLERMRRA"
     gene            complement(26705..30487)
                     /gene="hrpA"
                     /locus_tag="VKZ48_07560"
     CDS             complement(26705..30487)
                     /gene="hrpA"
                     /locus_tag="VKZ48_07560"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HLU77742.1"
                     /translation="MSAPAPLKRRSRPNDRPRSLRPEVIEARRANLPRPSFPEELPVS
                     ARREEIARAIEAHQVVIVSGETGSGKTTQLPKICLSIGRGVHGYIGHTQPRRLAARAT
                     ASRIAQELQSELGQAVGYKIRFNDRTSPSSYIKLMTDGILLAETQADPELRAYDTLII
                     DEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATLDADRFARHFAQANGEPAPVIEV
                     SGRLYPVEIRYRPPQENEDGEIDINEAISDAVDELAHLGPGDVLVFLPGEREIREAAE
                     ALRKHHPPHTEILPLYARLSAAEQERVFKGHAGRRIVLATNVAETSLTVPGIRYVVDT
                     GLARIKRYSYRNKVEQLRVEPVAQSAARQRAGRCGRVAAGVCIRLYGEEDYEKRPSYT
                     DPEVLRSSLAAVILRMKALRLGAVEDFPFIDAPAPKAVTDGYALLQELGAVDEARELT
                     DIGRQLARLPLDPRIARMVLAARQEQCLTEALVIASALSVQDPRERPAERAQAADEAH
                     KKFADEKSDFLGFLKLWKFYEDTLHRQSNRKLHRACRDHFLSYLRMREWRDVHTQLKE
                     LVSEQGWRPNEKPATYAQIHRALLSGLLGNAGLKQEDGQYLGARGIKFLIHPGSALAR
                     KAPRWIMAAELTETTRLYARAVAGIEADWLESVGAHLVKRHRYEPHWEKKPAHVAAFE
                     RATLYGLPIYTHKRIHYGPLDPETSREIFIRQALVAGEYDTRAPFFQHNRRLVAQIEA
                     LEHKSRRPDVLVDDELIYAFYDSLIPAGIHNGAAFDAWRKEAEAQNPKLLFLRREDLM
                     RHEAAGITTAQFPPALEMAGRSFALEYLHDPGGPRDGVTMTVPLIALNQVSAARCEWL
                     VPGLLKEKVMQLAKSLPQRLRHRLGPLQEFAAAFVADVAPSDNALAEAIARYARSERQ
                     LVLPLDTFRPDTLPPHLIMNFRVVDEHGRQLAMGRNLAALRAELGHKAGEQFAAVAQP
                     RAAMDGLTDWSFGELDEVMEIRSGSQILIGYPALVDEGDSVSLQVFDAQDKARATHRA
                     GLRRLFMLQLKEQAKYLEKNFPGFQTMALQYAALGGEAADLKRQLLAATFDRACMAEP
                     WPATREQFEQRRDEGRTRLSLIGQELARLIGVILTERQSVSKKLQTLKAYPEVVRDIE
                     DQLARLLPKNFIEDTPYERLQHFPRYLKAIALRLDKLRTDPARDARAMSEYAPLQAQW
                     QREDAKRRKQGEADPQLEQYRWMLEELRVQLYAQELRTPAPVSVKRLQKAWEALRR"
     gene            30569..31843
                     /locus_tag="VKZ48_07565"
     CDS             30569..31843
                     /locus_tag="VKZ48_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HLU77743.1"
                     /translation="MNILLCGANGFIGRHLAAALEGRGHRVVRGVRDTEDGKDAVAID
                     YTRDHGPDIWRPRLEGIDVVINAVGILRERGTATFGALHARAPIALFDAAAQVGVRRV
                     VQISALGADEGAQSRYHRSKKQADDYLATLPLDWVVVQPSLVYGPGGTSAKLFDTLAA
                     LPLIPLPGGGRQRIQPVHIDDLVEAIVRLVEYDGPLRGIFPAVGAEALSFGEWLRAVR
                     AQLGLPHTLALPIPAWMMRIAAALGERLPGLLDRESLTMLERGNTAPSDAMTTLLERP
                     PRPVTTFIAPERARETALLARLAWALPLLRFSVAAVWILTGLVSLGLYPAASSYALLA
                     QVGVTGALAPVTLYGAATLDLAFGIGVYALHPPRRRWLWRAQMLLIVGYSLIIAWRLP
                     EYWLHPFGPLLKNIPLLAVIVLLHEFDDTDEH"
     gene            31843..32313
                     /locus_tag="VKZ48_07570"
     CDS             31843..32313
                     /locus_tag="VKZ48_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 domain-containing protein"
                     /protein_id="HLU77744.1"
                     /translation="MEYLIVKWLHILSSTLLFGTGLGSAFYMYFTNRTRNVEAMAIVT
                     RKVVAADFLFTTPTAILQPLTGFWLVQLAGFPLTAPWILWSVVLYVIAGACWLPVVWI
                     QLRMAKLAEAAAAARQPVSAEYWRLHAVWVLLGVPAFIAFIGIFYLMVVKAPPF"
     gene            complement(32280..32762)
                     /locus_tag="VKZ48_07575"
     CDS             complement(32280..32762)
                     /locus_tag="VKZ48_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77745.1"
                     /translation="MGAQDLLPRALVSGTVAALTSAVAASWCGRKAGHAAGPLNAVSH
                     IAWGGSARSQARHDGVNTAVGFALHHGASIFWALGFEALFGRRAERSVPDALVGGAAT
                     AVTAYVVDYHVVPERFVPGFEARLEGGSLFYVYAALALGLAAAAQARAQKGGAFTTMR
                     "
     gene            complement(32765..>33307)
                     /locus_tag="VKZ48_07580"
     CDS             complement(32765..>33307)
                     /locus_tag="VKZ48_07580"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HLU77746.1"
                     /translation="LAGAGLLYHAWTGRIPFAESVAGAALEPMLVSDSVQVGAGRDST
                     YRFWRNVQNLPSIMKRVERVEQLDQRRSRWTVRTAQGQVVSWVSEITDDDPGKAIYWR
                     SVDSPVRHEGVVRFEDVPNDALATKVTVEMRYMLPAGLRGEMARQALGDPQRDLHEDL
                     QRLKQTLEHEDFLMAAPPGA"
BASE COUNT         5974 a        11003 c        10567 g         5763 t
ORIGIN      
        1 gtgaaaatgc cgccgcccaa tcgcgcgaag atcgagatga gcgagccgcc gaagcccagg
       61 cccaccagcg gtttgatgag gtcggcctgg gacgtctccg gtccacccat tgccgcaagc
      121 acggcataga agccggccac accgagcagc cccagtccga ccaccagcag gccggtgatc
      181 gcgccgcccc gaaatgcgac cgacaaggct tcattgaggc ccttgcgcgc cgcctcggcg
      241 gtacgcacat tggcgcgcac cgaaacgtgc atgccgatat agcccgccgc gccggagagc
      301 gccgcaccga tggcgaagcc gacagccgtc gacagtccca gcgcaagcca gatgacgatc
      361 agcaatacga cgccgacgat gccgatcgcg gtgtactgcc ggttgagata ggcggatgcg
      421 ccctgctgaa tcgcgccggc gatttcccgc atgcgctcgt tgccgctggg ttgcttgagg
      481 atccagctga tggagatgat gccgtacacc agcgccaaaa cggcgcagac caacgcaaat
      541 aacaaacccg cactcattca gacctcctcc ctgtggtgga gctactgctg ctgcgtgttg
      601 ttgctgcccc accggttgac gaagtgaagc acggcgacgc ccggtcatcc cgggcgacac
      661 gtcgcggcgt tgcgttcgta catcgtgatt tctggacgcg gcgtgtacct gcggcggatg
      721 ccgcaggctc cggcgcatta tatgcgcgct tgcgcaggcg acgggaaaga gattggaaag
      781 ctgctgcggc ggcgccgcac ggccgtgccg ctctgtcgaa agcggcacag tccgcgcgcg
      841 accgctagcg gccgagcttt ttggggacgg gcgcgttctt gaggcgcacg tagtccggca
      901 agccgttgcg gaagggcggc ggcgcttcgc cgcgaatcag cggggcaaga tagcgccggc
      961 aggcatcggt gatatgaaag ccgtcgcgac tgatgtagct gcgcggcatt ttcttttcct
     1021 tgttggctac gtccttcaac ggcgcggtgc cgatcttcca gcgataaggt ttgtcggaaa
     1081 cgcgcacgat ggtcggcatg accgcattct gtcccgccag cgcgagcttc accgccgcga
     1141 cgcccagcgc ataggactgc tccacatcga ccttcgatgc gagatggcgc gccgcacgct
     1201 gcagatagtc ggccaccgcc cagtgattct tgtagccgag ttcatcggtg atcatctgcg
     1261 cgatgcgcgg tcccacgccc cccagttgca catgaccgaa ggcgtcgcgc gtgccggctt
     1321 cggcaagaaa acgcccgtcg gcatccttga gcccttcgga gacggcgatg gcgcaccagc
     1381 ccacgcgctt caccgtccgc tccacgcgcg cgaggaactt cgccttgtcg aaggtgatct
     1441 cggggaacag caggatctgc ggcgcctcgt cggacgactc ggcggcaagg ccgcacgcgg
     1501 cagtgatcca gcctgcgtgc cggcccatca cttccagcac gaacacctgc gtggaggtgg
     1561 aatgcatgga ggccacatcg aaacctgctt cgcgcatgga agtggcgacg tatttggcca
     1621 ccgagccgaa gcccgggcag ttatcggtga gcgccagatc gttgtcgacc gtcttcggca
     1681 cgccgatgca ggcgatggga taacccagtt cctcgccgat gcgcgacagc ttccaggcgg
     1741 tgtccatgga atcgttgccg ccgttgtaga agaaggtgcc gatgtcgtgc gcgcggaaga
     1801 cttcgatcag gcgctcgtat tgcgagcggt cttcctcgag ggacttgagc ttgaagcgcg
     1861 ccgagccgaa ggcgccgccc ggcgtgtgct tgagtgcgga gacggcgcgc gcagactccc
     1921 tgctcgtatc gatcaggtct tcattcagcg cgccgacgat gccgttgcgc gcggcgtaga
     1981 gcttgccgat gcgccccttc gccgcgcggg cggtttcgat gacgccgcag gcggtggcat
     2041 taatgacggc agtgacgccg cccgactgcg cgtacagtgc gttcttcggt ctggccatga
     2101 cgatctcttc aggattcgat tacgcggcgc gccgcatata ccgggccgac tgcctcgagt
     2161 ggagaaccaa aagcttgaaa cacagagagc acggagaagc ggattggttc gctcagtgtt
     2221 ctccgtgatc tccgtgtttc aatttcttag cgcagggccg cgaacaccgc ctcgcggatc
     2281 gcctcgacgc tgccgacgcc ttcgatacgg cgatagcgcg gcgccttcgg gtcgcccgag
     2341 gccgcccact tcgagtagta gtccaccagc ggacgggtct gctcgcgata gacctccagg
     2401 cgcttgcgca ccgtctcttc cttgtcgtcg tcgcgctgga tgaggtcctc gcccgtcacg
     2461 tcgtccttgc cttcgacctt gggcggattg aacttgacgt gataggtgcg acccgaggcc
     2521 gggtgcacac gacggccgat catgcgctcg atgatgctcg catcgtccac gtcgatctcg
     2581 agtacgaaat cgagcgccac attcgcttct ttgagcgcct cggcctgcgg aatggtgcgc
     2641 gggaaaccgt cgaacagata gccgttgtgg caatccggct cttgcagccg atccttcacc
     2701 agtccgatga tgatgtcgtc ggacaccaat tgccccgcat ccatcacctt cttcgccgcg
     2761 agaccgagcg gcgagccggc cttgacggcc gcgcgcagca tgtcgccggt ggaaatttgc
     2821 ggaatgttga agcgctcctt gatgaacgcc gcctgggtgc ctttgcccgc gccgggcgga
     2881 ccgagaagta tcaagcgcat gatttgttgt tccttgctga gaggtttcgg gcccggctag
     2941 gaagccgggg cgaagcgcgc gcgcacgcgc gccaggtctt cgggcgtatc gacgcccgcg
     3001 ggcggcgcct cctcggtcac caccatggcg atgcgatgac cgtgccacag cgcgcgcaac
     3061 tgttcgagcg actcgaagcg ctcgagcggc gccggcgcga gctgcgtata ggcgcgcaag
     3121 aacgccgcgc gataggcgta catgcccaga tgccggtacg cgggaaactg cgccggcagg
     3181 ccgggcgcat tgccgacgaa ggcgtcgcgc gcccagggaa tgggcgcgcg actgaaatac
     3241 atcgcatcgc cctgacgatc gcagaccacc ttgaccacgt tgggattgaa gaacgtctct
     3301 tcgtcctcga tggcgtgaca gacgctcgcc atgtcggcgt cgggacgtgc ggccagcgcc
     3361 tcggccaggc gctcgatcaa cgccggcgga atgagcggct cgtcgccctg cacgttgacc
     3421 acgatcgcct cgtccgccag accgatggcg tccaccactt cggcgatacg gtcggtcccc
     3481 gagggatgat ccgcacgcgt gagcagcacc tccatgccgt gcgcccgcac ggcctcggcg
     3541 atcgcttcgt gatcggtggc caccagcacc gccgcggcgc cgctcttgcg cgcctgctcg
     3601 gccacgcgca ccaccatcgg cttgccggcg atgtccgcca gcgccttcgc cggcagacgc
     3661 gtggacgcgt gacgcgcggg aatgacgacc gtgaacggaa cgcgggccat gcgtccgcta
     3721 agcgatgtct tcggcggggt cgagcttgcg cgcttcgtct tccagcatca ccggaatgcc
     3781 gtcgcgaatg ggaaaggcga ggcgatcggc cttgcagatg aattcgccct cggccttgcg
     3841 ataaagcagc gggcctttgc acaaggggca gacgaggatg tcaagcaggc gcggatccat
     3901 gcagtcgggt ctcaatcagg tcggcaagcg cgggatcgac caccgcctcg acgcgcaatg
     3961 caacgagatc ggtgcgaccg aatccgcggc attttactgc atccttctcc gtcatcagta
     4021 ccgcatcgca gtcgggataa cgcaagtccg cgtccgcgta gcggtgatga tcgggaaaag
     4081 catggctctc gacgcgcaag cccatctccg ccaactgacg gaaaaagcgc tcgggattgc
     4141 cgatgcccgc cacggcatgc aggcgcatgc cgcgcagcgc ctcgagcgga acggcggccc
     4201 cgtcgtgcac ccgataaaag ccggccgatt cgagccgcat ggcataggcg ccgggccggc
     4261 tgccaccgtt gaccaccgtg gcatcgacgc gccgcgaggc cggctcccgc aagggcccgg
     4321 ccggcagcag ccggccgttg ccgtggccgc gttcgtcctc caccgcgatt tcgacgtccc
     4381 gcgcgagagc gtaatgctgc aagccgtcgt cgcataccag cacattgcag tccggatgcg
     4441 ctgccaggag gacgcgtccc gcatcgacgc ggcggcggcc gatccatacc ggcacgccgg
     4501 cacgcgccgc cagcagtgcg ggctcgtcgc ccacttgcgc gggatcggcg ccgggtgtca
     4561 cggcggaagg tgcggcgttc tgtccctggt agccgcgcga cagaatgccc ggccgccagc
     4621 cgcgcgcccg caactgttcc accagccaca gcaccaacgg cgtcttgccg gcgccgccgg
     4681 cgaccaggtt gcctacgatg acgactggca cggacagacg ctcgctgcgc agccagccgc
     4741 gccggtaagc gcagcggcgt gcggcggcga cggcggcgaa caggaaggag aacggcaaca
     4801 gcgccaggga gagcgcggat cggccgtacc acacgcgctg cgccagaccg ctcgcaccgc
     4861 gcatcatgcg gcgcgcctat tgttccgtgg actgcgtcgc gaacgtgaca tgggtgtagc
     4921 ccgccatgcg cgccgcctcc atgacatgca ccacggactg atggctcgcc ttggcgtcgg
     4981 cgctgatgat caccaccgga ttctcgagat cgcccgccgc gcggcgcatc gcgtcgctca
     5041 ggctgggcac gttattgaag ggcgtcgcac gcccgttgat ggtgtaggcg ccgagcgcgt
     5101 cgacggtgat gttgagttca ttggggcgct cgacctgctt ttccgcattc gccgtgggca
     5161 ggttgatctg cagttccgag tatttcgagt aggtcgtggt caccatgagg aagatcagga
     5221 tgaccagcaa tacgtcgatc agcggaatca ggttgatttc cgggtcttcc ttccagcggc
     5281 tgcggaattt catgccagag aggctctatt caaggtttgc cggcaacgcg gtcgacaatg
     5341 catgcaccgg cacgttgata ttccatttcc ctcgccccgc actagtcctt ccgggtaccg
     5401 tgcagcatgt ccaccaggcg cgaagcctgc tgctccatgg tcacgacaaa atcctcgacc
     5461 ttgccgcgga aatggcggaa gaagatggtg gcgggcacgg cgatcagaat gccgaagcca
     5521 gcgttataga gcgcgatgga aatgccgtgc gcgatctgct gcggattggc gccgctcggg
     5581 ttctgcgagc cgaagatctc gatcatgccg acgatggtgc cgaacagccc ccacagcggg
     5641 ctgatcgagg cgatggtgcc gagcgtggtg aggaagcgct ccagttcgtg cgtgacggca
     5701 cgcccctcct cttcgatggc ctccttgatg accggacgcg gcgcaccgtg attgcgcaac
     5761 cccgccgcca gcacgcgccc caggggcgag tcggcataga ctgcatcgac gagttgctgg
     5821 gtcacgccct gcttgcggta ggcctcgacc actttctcca gcaggttgtg cggaatgatt
     5881 ttcggcgcgc gcagcgcgat cagccgttcg atgatgagtg cgaccgcaat aacggaggca
     5941 ataagcaaaa accagatcgg ccatccggca gcctcgatga tttcgaacac gcactcactc
     6001 ctgctcgcaa aggttgctga atgtagcggc gcaggtacga acgatcaagc caagtggtac
     6061 gcacaacgtg caatgtgcgg aacgcggttc aaaaaatcga cggcgcagca ccgttcgatt
     6121 gaaccgaaag gcttgacaga ttcgtaaagc gctccttatc cacagttgct gtggataagg
     6181 ttgtgtatag cccttacaca aagcagctaa gtggattgct cataaagact ttttctacgg
     6241 tgatcatttt atgatcagcc aaaaaagttt tttctttcat aaacttgcac cgcgctccct
     6301 agggagaagt ttcgtcgagg acgcgttgcg caagaacagt gccggtttgt ggatacattg
     6361 cgccgcgacg ccaccaaaac cccttgtacg acaatgcgtt tcaacgcccg ccgcaggtgg
     6421 cgcggacacc tgcattgcgt agaatccccc gatgcctgaa ctgcctctta cgccgtccgg
     6481 ccgaccgacg gccgaaacca tcctgagcgt cagcgcgctc aaccgcagcg tccgcgatct
     6541 gctcgaacac cgcttcccgc tcgcctgggt cgccggcgaa atctccaatc tcacgctggc
     6601 ccgctccggc cactggtact tctcgctgaa ggacgacaac gcgcaagtgc gctgcgtgat
     6661 gtttcgccat cgcgcacagt ttctgaactg gcagccgcgc gaaggcatgc acgtggaagc
     6721 gcgcgcgctg gtcacgctgt acgaggcgcg cggcgacttc cagctcaaca tcgagttcat
     6781 gcgtcaggcc ggacagggcg cgctgtacga agcctttttg cgcttgcgcg acaagctcgc
     6841 agccgaagga ttgttcgacc cggcgctgaa gcgctcgctg ccgcccttcc cgcgcgccat
     6901 cggaatcgtc acctcaccgc atgcggcggc gctgcgcgat gtgctgacca cgctcgcccg
     6961 ccgcaacccg tccattccgg tgattcttta tccgacttcg gtgcagggcg aagcggcgcc
     7021 cgccgaaatc gtcgccgccc tgcgcgccgc cagccgccgc gcggaggtcg atgcgctgat
     7081 cctttgtcgc ggcggcggca gcatcgagga tttatgggcg ttcaacgacg aagccgtggc
     7141 gcgcgccatc cgcgagtgtg tcgtgccggt ggtctgcggc gtgggacatg aaacggatgt
     7201 gaccatcgcc gattacgccg ccgaccagcg cgcgcccacg cccaccgcgg ccgccgaatt
     7261 gctgagcccg gatcgcgcga gcctgctcga acgcatcgcc caattgcgcg gcacgctgca
     7321 gcgctgcatg acgcgactgc tcgaaagccg catgcaacac gtcgatcatc tgagtcggcg
     7381 tctggtgcat ccgggcgagc gcctgcgggc gcagcagcgc ctgctcacgc agttgtgcag
     7441 ccgtctgtca cacgccgcca cgcgcgatct ggagcggcgc cactggcagg tgcacagcct
     7501 gctggagcgc ggccgtcgcg cattacccaa cgtcgacgcg cgccgcgccc gcctgagcga
     7561 actgatgcat cgtctcgccg ccgcatcgac gcgcggcctc acggcgcacg atgcgcgact
     7621 cacgcacctg tcgcgcgcgc tggcgcatct ggatccgcgc gccgtgctgg agcgcggcta
     7681 cagcctggtg acggacgaga cgggtcgcgt agtgcgggac gcgcatgcgc ttgaacgggg
     7741 cgacggcctg cacattgcat tcgcccgggg cagcgcactg gcgcgcgtgg aagacactcg
     7801 cgaagactga ccgccacgca agcgcggttg catgggtgcg cttcgccttc tatgatcgag
     7861 tgttccgatt ttttgaccca aatcacatat ccaggagacg tacatggaac acaagctgcc
     7921 tcctctgccc tacgcaatgg acgcgctgca gccgcacatg tccaaggaaa cattggagtt
     7981 tcactacggc aagcatcacc aggcctacgt gaccaacctc aacaacctca tcaagggcac
     8041 cgagttcgag aacgccagcc tggaagacat catcaagaaa tcctccggcg gcctgttcaa
     8101 caacgccgcg caggtttgga accacacctt cttctggaac tgcatgtcgc ccaacggcgg
     8161 cggcgcgccc accggcgcgg tggccgatgc gatcaacaag aagtggggct ccttcgatgc
     8221 cttcaaggaa gccttcaaca agtccgcggt cggcaacttc ggttcgggct ggacctggct
     8281 ggtgaagaag gccgacggct cggtggatat cgtcaacacc agcaatgccg ccacgccgct
     8341 gaccaccgac gacaagccgc tgctcacctg tgacgtatgg gagcacgcct attacatcga
     8401 ctatcgcaac cgccgccctg atttcgtcgg cgcgttctgg aatctggtga actgggagtt
     8461 cgtcaacaag aacttcgcct aagccacgcc agagcgggaa gcaaagggct ccgtgaggag
     8521 ccctttttca ttccgccgcc ggcagcttgt ccagcccgcc gatacgcgca ccgggcgcca
     8581 ggcgccgctg cgcgaaccag cctcgattga cctccagcgc gtagcgcacc ggcttggcgg
     8641 cgcagtggat atcgtccgtg cgaggcgtca tgtcggcgat attgacgatg cgtccgtcgt
     8701 cgtcgatgaa agcgaccgac agcggcagat gcgtgttgcg catccacatg caatggcgac
     8761 ctgcttcagg ccaactgaac accatgccgt ggttgtcctc catgctgcgc cgattcatca
     8821 gccctttggt gcgcgcctga tgggtgtgcg cgacctccgc ctggatgcga tgaatgccgg
     8881 cattgagatc caccaccggc atttccgtcg ccatgaccga cgctgcgtac agcagcgccc
     8941 ccgcgatcaa cagtccacgc aacatccctg ctcccttgtc gtcctcgttg tgaatatcag
     9001 cgcagatcgc ctgcgcgcac ctcgacccag cgccactgtc ccggctgcaa tccttccacc
     9061 gaccacggtc cgacggcggc acgcaccagc cgaagggtcg gcaaccccac tctggccgtc
     9121 atgcgacgca cctgccggtt cctgccctcg aaaagcacca gttccagcca ggtcgtcgga
     9181 atcgcctgac ggaagcggat gggcgggtgg cgcggccaca agcccgccgg ctccgcgatg
     9241 gcgcgcgccg cggccgggcg cgtcacataa tcgcccagat cgacgccttc gcgcagttgc
     9301 gccagttgcg ccgcctcggg caccccctcg acctgcgccc agtagatctt gccgagcttg
     9361 tgccgcggat cggcgatgcg ctgctgcatg cggccgtcgt cggtcagcac cagcagccct
     9421 tcgctgtccg catcgagccg tccggcgggg tagaccgccg gcacgtcgat gtagtcggcc
     9481 aggcactcgc gctcgccctc gcgcgtgaac tggcacagca cctgataggg cttgttgaac
     9541 agcagcacgc gcgatgcgcc gtgcggacgc ttagtacgtc ccgccggctc tgctaaaatc
     9601 gcccgcttcg cgcgcgggcc ggacaaagct ttggtgctgc gcatgccgcg cgccttcagt
     9661 ccaacggtta gaggagtccc ccgtggccaa aatcaaggtg caaaacccga tcgttgaaat
     9721 ggacggggac gagatgacgc gggtgatctg gcaacgcatc cgcgaccagc tgatcctgcc
     9781 ctacctcgac atcgaactca aatacttcga tctgggcatt gaaagccgcg atcgcaccaa
     9841 cgaccagatc accatcgacg cggccaacgc cacccgtcag tacggcgtcg ccgtcaagtg
     9901 cgcgaccatc accccggacg aagcgcgggt gaaggaattc aatctcaagc agatgtggaa
     9961 gagcccgaac ggcacgatcc gcaatatcct cgacggcacc atcttccgcg aacccatcat
    10021 ctgtcggaac gtgccgcgcc tggtgtccca ctgggacaag cccgtggttg tcgcccgtca
    10081 cggcttcggc gaccagtacc gcgccaccga gcacaaattc gagggagccg gcacgctgaa
    10141 aatcgtgttc gagcccgccg atggcggcgc gccggtggag aaggaagtgt tcaaggcgcc
    10201 gggcgccggg attgccatgg cgatgtacaa cctggacgac tcgatccgcg gcttcgcacg
    10261 cgcctgcttc aactacgccc gccagcgcaa ctacccggtc tacctgtcga ccaagaacac
    10321 catcctcaag acctacgacg gccgcttcaa gaacatcttc caggaggtct acgaggcgga
    10381 gttcaagcag gcgttcgagg gcgcggggct cagctacgag caccgcctga tcgacgacat
    10441 ggtcgcctcg aacctgaaat ggaacggcgg atacctgtgg gcgtgcaaga actacgacgg
    10501 cgacgtgcag tccgacacgg tggcgcaggg ctacggctcg ctggggctga tgacctcggt
    10561 gctgatgtcg cccgacggca agaccgtgga agccgaagcg gcgcacggga cggtcacccg
    10621 ccactaccgc cagcaccagc agggcaagcc gacctccacc aatccgatcg cctcgatctt
    10681 cgcctggacg cgcggcctgc agcatcgcgg acgcatggac agcacgccgg aggtggtgaa
    10741 gttcgccgag acgctggaac aagtctgtat cgacgtggtc gagtccggcc gcatgaccaa
    10801 ggacctggca atcctgatcc gtcccgatca cccgttcctc acgaccgatc agtacatgga
    10861 tgcggtgaac gaggaactga agcggcggat ggccgcctga taaacggccc cgtccgcgcg
    10921 acggggcctt gaaaagccgc gagggcgacc ccacaaataa aaagagcccc gccgcgtggc
    10981 ggcgaggctc aaaatttttt tattgtgttt gggtggtgcg gattcggctc aacttgctga
    11041 ttggatgttc gaggcctgct tgcccttcgg gccttgcgtg acttcaaact ggaccttctg
    11101 gccttccttc agcgttttga atccgttcat ctggattgcg gagaaatgtg caaacaaatc
    11161 ctcgccacca tcgtccggag taataaagcc gtatcccttt gcgtcattga accacttcac
    11221 cgtaccggtt ggcatagccc cttattccct gaaaattaac aaccggggat tccccctcct
    11281 atgcctgtac tccaagacag ctccaggacg aaaacgacct caatactgct tgaaaaacaa
    11341 acacacgcgt tttttacgcg ctttgcggaa ccccgtcaag cctgcgctgt caaggggctg
    11401 ttgcagtcgt tgcacggcac tagaacagtg gctaggatac gattcgagag ccctgacgga
    11461 ccgagcaggc cggtttgatc cggtactcag cttgctgatt gctcaggaaa gtcgcagcaa
    11521 accgtcgaga aagtatggca acacgttccg gtgaaggcac gatccttgaa cccagcaaga
    11581 gcaaggtcgc accgccgccg ctttttaaag tcattctgct caacgacgac tacacgccga
    11641 tggagttcgt ggtggtcgtc ctgcaaactt tcttctccat ggatcgtgag cgggccacgc
    11701 aggtgatgct ggcggtgcac cgggaaggta tgggggtttg cggcatttac ccgcgcgata
    11761 ttgcaacgac caaagtggaa caagttactg cctacgcacg tgagcatcag cacccgctcc
    11821 agtgtgtcat ggaggagacg tagatgatcg cccaagagtt ggaagtcagc ctgcacatgg
    11881 cgttcgtgga agcgcgccag aagcggcacg agtttattac ggtcgagcat ctgctcctgg
    11941 cattgctgga caaccccacc gccgccgaag tgctgcgcgc ctgcgccgcg aatctggacg
    12001 aactgcgcaa ggcgctgacc gaattcatcg gcgcccacac gcccacggtc gccggcagcg
    12061 aggaaatcga cacccagccc acgctcggct tccaacgcgt cattcagcgc gcgatcctgc
    12121 acgtccagtc ctcgggcaag aaggaagtca ccggcgccaa cgtgctggtc gcgatcttcg
    12181 gggaaaagga ttcgcatgcc gtgtacttcc tgcatcagca gggagtgacg cggctggatg
    12241 tggtcaatta catctcccac ggcatcacca aaagcggcca gccgagccag cccaaggcga
    12301 ccgaggaggc ggagcagcag gaagaaggcc agcaatccgg cggcgcgctg gagacctata
    12361 cggtcaacct caacgcacag gcgctggtcg gcaagatcga tccgctcatc ggccgcgacc
    12421 gcgaggtgga gcgcgtcatc cagacgcttt gccgccgccg caagaacaac cccctgctgg
    12481 tcggcgaggc cggtgtcggc aagaccgcca ttgccgaggg cctcgcgcgc cgtatcgtcg
    12541 agggccaggt gcccgacatc ctggcgcaat cgacggtcta ttcgctcgac atgggcgcac
    12601 tgctcgccgg caccaaatac cgcggcgact tcgagcagcg cctcaaggcc gtgttgaagc
    12661 agttgctgga caatccgaac gcgatcctgt tcatcgacga gatccacacg ctcatcggtg
    12721 cgggcgccgc atcgggcggt acgctggacg catcgaacct gctcaagccg gcgctttcca
    12781 ccggccagct caagtgcatc ggcgcgacca cctatcagga ataccgcggc gtgttcgaga
    12841 aggaccacgc gctgtcccgc cgtttccaga agatcgatgt gaacgagccg tcgatcgagg
    12901 agaccgtgga aatcctcaag ggcctgaagt cgcgcttcga ggcgcaccac ggcgtgaagt
    12961 acagcgccaa tgcgctgtcc accgcggcgg agctgtccgc gcgcttcatc aacgaccggc
    13021 acctaccgga caaggccatc gacgtgatcg acgaggccgg tgccgcccag cgcgtactgc
    13081 ccaagtcgaa gcagaagcgc gtcatcggca agccggagat cgaggagatc atcgccaaga
    13141 tcgcccgcat tcccccgcag acggtgtcca gcgacgaccg cgccgcgctc aagaatctgg
    13201 accgcgatct caaggccgtg gtgttcggtc aggacaaggc catcgatgcg ctggtgacgg
    13261 caatccgcac ggcgcgctcg ggcctgggca gccccaacaa gcccatcggc agcttcctgt
    13321 tcagtggccc gacgggagtc ggcaagaccg aggtggcccg acaactcgcc tactgcctgg
    13381 gcgtggagtt ggtgcgcttc gacatgtccg aatacatgga gcgtcatgct gtatcgcgac
    13441 tcatcggcgc gccgcccggc tacgtcggtt tcgaccaggg cggcctgctc accgaagcga
    13501 tcacgaagaa gccctactgc gtgctgctgc tggacgagat cgagaaggcg catccggaca
    13561 tctacaacat cctgctgcag gtgatggacc acggcacgct gacggacaac aacggccgca
    13621 aggcggactt ccgcaacgtg acgatcatca tgacgaccaa cgcgggcgcc gaagcgctga
    13681 cgaagaccac gatgggtttc gctgcaccga cggccgaagg cgacgaaatg gcggatatca
    13741 agcgcctgtt cacgcccgag ttccgcaacc ggctggacgg catcatctcc ttccgccagc
    13801 tcgatcacga catcatcctg cgcgtggtcg acaagttcct catgcaactg gaggagcaac
    13861 tgcacgagaa gaaggtggaa gccatcttca ccgagaagct caaggactat ctcgccaagc
    13921 gcggcttcga cccgcttatg ggtgcgcgac cgatggcgcg actcatccag gacaccatcc
    13981 gcaaggcgtt ggccgacgaa ctgctgttcg gcaaactggc caacggtggc aaggtcacgg
    14041 tggatctgga cgacgacgac gaggtggttt tgcgcttcgt ggaggaagaa gaagaaaccg
    14101 ccacgccttc cgcttaacgc tctgagttat aagcaaattc tcaaaggccg caatacgcgg
    14161 ccttttttct tttttggctt gacggttgca ttcaagcgcc ggagactcgt ggtttttctg
    14221 tctaggccga aggagatttt taaatgaacg gaaggctgaa tctcggcctg cggctgtgtg
    14281 cgttacttgc ctttgcgccc gctgcggcgc tcgcgcaaaa cgccgaatcc aatacggcgc
    14341 gcgatcttgc cgccgcctgc gccatctgcc acgggaccaa tggtttcaac gcgggcgggc
    14401 ttccccacct ggccggtcag ccgaaggatt acatcgtgca gcagttgaag gacttccgcg
    14461 acggcaagcg gccggcgacg atcatgcatc aggtcgccaa gggctacacc gaagaacaat
    14521 acgaactgct gggcgcctgg ttctccgcgc agcagcgcaa gaagtaaggg gccggcatca
    14581 tgagtttgca aatggatcgt cgcggtttca tcaagttgat cggcgccggc gccgcagcca
    14641 ccatggtgag cggctgcgcc tcggtcggcg gcaaatcgag cgtcaagtcc gccgggcgcg
    14701 tcgtcgtggt cggtggcggc tacggtggcg ccacggccgc gaaatacatc cgcatgtgga
    14761 gcggcggcgc gatcgaggtc acgctgatcg aacccaacac ccagttcatc tcctgcccgc
    14821 tatcgaacct cgtgctcggc ggcgcacgca cgctcgacag cctcaccatg ggttacgaca
    14881 cgctgcgtgc gcatggcgtg aaagtggtgc acgacgtcgt gaccactgtg caggccgata
    14941 agaagcgcgt gacgctcggc aagggcggcg agcttgcgta cgaccggctg atcgtctcgc
    15001 ccggcgtgga cttcatgtac gacaagctgc cgggcctgaa caatgctgcg gcccagcaac
    15061 aggtattgca tgcatggaaa gccggtccgc agacgctcgc gctgcgcaaa cagttggaag
    15121 ccatgcccaa cggcggcgtg tacgtgctgt ccattcccgt cgcaccttat cgctgcccgc
    15181 ccggcccgta cgagcgcgtg tgccaggtgg cgaattactt caagcaggcg aagccgcgtt
    15241 ccaaggtact ggtgctggac ggcaatccgg acatcgtctc caagaagggc ctgttcctcg
    15301 ccgcatggaa ggagctttat ccgggcctga tcgaatacgt gccgaacgaa gtcgcgcgcg
    15361 acgtggacgt gaagacgcgc accatcaaga ccgagttcga aagcttcaaa ggcgacgtgc
    15421 tgaacgtgct gccaccgatg cgcgccggca acatcgccgc atcgtccggt ctgatcacca
    15481 ccaacgaccg ctggtgcgac gtgaactggt tgaccatgga atcggtggcc gtgccgaacg
    15541 tgcacgtgct gggtgactcg accctgtctg ccccggcgat gccgaagtcc ggcagcatgg
    15601 ccaacaacca cgccaagatc gccgcgtccg cgatcgtcgc gctgttgacc ggccagacgc
    15661 cgaaccccac gccgatcatc ggcaacacct gctacagcta cgtgagcgat cggctggcga
    15721 tgcacgtcaa ctccgtacac cgctacgacg cggagaagaa aacgctcctc ccggtggccg
    15781 gcagcggcgg ggtgtccgcc aagcacagcg aactggagaa atcctacgct gatgcctggg
    15841 cgcgcaacat ctgggcggac atgctggttt gatccgtgct cgcaccaaaa caaaaaggcg
    15901 gcttcggccg cctttttgtt ggttgcgcga aacgccgatt tcaggccgtt ccagtgctgc
    15961 cgaaaccacc cgcgccgcgc tcgctttcct cgaaggcttc gaccacgttc aacgcgacct
    16021 gcagcacggg cacgatcacc agttgcgcga tgcgctccat gggttgcagt gtaaacggcg
    16081 tattcgaacg gttccacagg gacaccatga cctgtccctg gtaatcgctg tcgatcaggc
    16141 ccaccagatt gccgagcacg attccgtgct tgtgccccag ccccgagcgc ggcagcacca
    16201 tggcggcgag gcctttgtcg gccaggtgaa tggcgatgcc gctgggcacc agcgccgttg
    16261 cgccgggcag caggtcgagc ggcccgtcga tgcaggcgcg caaatcgagg cccgccgccc
    16321 cttccgtcgc gtactggggc ggttgatcgt gcaggcgggg atcgagaatc ttgacgtcga
    16381 tacgatgcat aagcgttgtt atagccttca ggagttgggc agacgcgcgg cgatttcacg
    16441 gatgaggccg cgcgccactt ccagcttgga cgcacggggc acgcgcgtct cgccgccctc
    16501 atgaaacagg atgagttcgt tctcgtcctg accgaaggcc cgatccgcac gattggccgc
    16561 cagcagcgga atgctcttgc gcaagcgctt ggcctgcgcg tactcgcgca ggttctccgt
    16621 ctcggcagcg aaacccacgc aataaggcgc atcggcgagc gcggcgactt cggcaaggat
    16681 gtccgggttc tcgctcagtt cgatctgcgg cttgcactgc gacttcttca gcttctgcgt
    16741 gctcgcgttg gcgacgtgat agtccgccac cgccgccaca ccgatgaaga cgtctgcctc
    16801 agtcacgcga tccatgaccg cgcgatgcat ctcccgcgcc gtggtgacgt tcacgcgctg
    16861 cgcgcccgtc ggcgtggcga gcgacgtggg accggcgaca agggtcacct gagcgcccgc
    16921 ctccaccgcg gcctgcgcca ccgcgaagcc cattttgccg gaactgagat tggtgatgcc
    16981 gcgcaccgtg tcgatcggtt cgaacgtggg tcccgcggta agcaatacgc gtttgcccgc
    17041 gagcggcttg tcgcccagcg cagtcgcgat atgcgcgtag agttgctccg gttcgagcat
    17101 gcggcccatg ccggtctcgc cgcaggcctg ttcgcccgca tcgggtccga gcagataaac
    17161 gccgtcggca cgaagttgtg cagcgttacg ctgcgtggcg gcgtttcccc acatctgccg
    17221 gttcatcgcc ggcgcgacat agagcggcgt ttcgcgcgca agacacagcg tggacagcag
    17281 atcgtcggca aagccgtgcg ccagcttggc catgaagttg gcggtcgccg gggcgacgag
    17341 aatgaggtca cggtcgcgcg acagttcgat atgcgccatg ttgttggcga tgcgcgcgtc
    17401 ccacatgtcc agataaaccg gcttgccgga cagcgcctgc agcgttagcg gcgcaacgaa
    17461 tctgcaggcg ctctccgtca tcaccacctg cacgtcggcg cccgccttga cgaacagccg
    17521 gcacagctcc gccgccttgt aggccgccac gccgccggtc agtcccagta gtattcgctt
    17581 gcctgtcagt tcactcatct tgtgcccgat catgcgtccg cccggcctag cccgatggtc
    17641 ggatcgcttc ccgaccggcg cacgcctact atccggcggc gattctacgc gccatttgcc
    17701 atgggaggga ccgatgggca tcgccgactg gccggaaggg gagcggccgc gggaaaaact
    17761 gctggaacgc ggtgcagccg ccctctccga cgcagaactg ctcgcgatct tcctgcgcgt
    17821 cgggacgcgc gggcgcagcg cggtcgacct gggacgcgaa ctgatcgcgc actttggttc
    17881 gctttcggcc ctgctgcacc atgcgcccgg cgaaacggcc gtcataaacg gcatcggtcc
    17941 cgccaaatgg gcccagctca aggccgcgct ggaactgggc cggcgcgcgc tggccgagac
    18001 ggcgcgcgag acggacgcgc tcagctcgcc ggccgccgtg cgggactacc tccgcctcct
    18061 gcttgcggag cgtccgcacg agatcttcgt cgcggttttt ctggacgcac agaaccgcgt
    18121 catcgcgagc gaagagctgt tccgcggcac cctgacccag acgagcgtct acccgcgcga
    18181 ggtggtcaaa gcggcattgc ggcacaatgc cgccggcgtc attctggcgc acaaccaccc
    18241 ttcgggcatc gccgaaccca gccagtcgga ccagttgctg acggatacgc tgaaacgcgc
    18301 cctggcaatg atcgacgtgc gggtgctgga ccacttcatc gtcggccggg gagcggcctg
    18361 ttccttcgcc gaacgcggca tgctgtgact tgcccggaac cgcatttgcc ctctataatc
    18421 cacggctttt gcgatttccg gagacgccgt catggcgcga gtttgccaag ttaccggcaa
    18481 gaagccgatg gtcgggaacc acgtgtccca cgccaacaac aagaccaagc gtcgcttcct
    18541 gcccaatctg cagtaccgcc gcttctgggt cgagagcgaa aaccgctggg tgcgcctgcg
    18601 cgtatccaat gccggcctgc gcctcatcga caagaacggc atcgagtccg tgctggccga
    18661 tctgcgtgcc cgcggcgaaa tctgagcgga gctgagtcat gcgcgagaag atcaagctcg
    18721 aatcgaccgc cggaaccggt cacttctaca ccacgaccaa gaacaagcgc accacccccg
    18781 acaagatcga aatcaagaaa tacgatccca aggcgcgcaa gcacgtcctc tacaaagaga
    18841 cgaagctgaa gtaagtcgtc cagcgttccg gaaagaaaaa cccgcctggc gggttttttt
    18901 attgcctact cgtcccgggg aaattccgga tcctcgggaa tcttgctcgg gtcgtagagc
    18961 gcggggtcct cgcccttgca gcgacgcttg cccgcacgct cggcctcgcg catctcctgc
    19021 gcttcctgct gcgattgcgg ggccgcctcg tcggcggcct gctccacctt gccggactgc
    19081 acgaactgct tggtgcgctc gttgtagtgc ttggcggcgg tgtagtcgcc ttcgccctgg
    19141 atcgggtcct tgtctgccat ctgcgtctcc tgaacgtttg acgtcctgaa aaacgcaacc
    19201 cgcgtgccgg tggcggggcg aaaaaacccg gcagagcgcc gggttttggt gagtgggctg
    19261 ccttaaagat caggcatagg accgcagccg catcgagaac tcccgcaacg gcgcgatgcc
    19321 gctcgcctcg gcgcgctggc accagtcctg cagttgcttg agcaactgct cgcgcgaagc
    19381 cgtggagcgc tcccacaggg ccgccagttc gcggcgcatg gaatagacgg ttttcagcgc
    19441 ttcgctcttg gccagcactt cctggacctt ggcgcgctgc tgctcgcaca gcttgctttc
    19501 atcccgcacc agccagcgct tcacgcgcca gttggcgggt tcgtgggcga gcttgctcat
    19561 ctcctgggcg aaagtggtct tgagcgactt ggcgtacttg gcccagacat cgtaacgatg
    19621 cgtcagcacc gcctgcagcg tctcgagatc cacggtggat ttggcgggcg cgaacttcgg
    19681 ccgcggcgcc accttcttca ccttggcaag tcccatcatt tccatgatgc ggatgtacat
    19741 ccagccgata tcgaactcgt accacttgga ggacagtttg gccgaggagg cgaacgcgtg
    19801 gtggttgtta tgcaactctt cgccgccgat caggatcccc caggggaaga tgttggtgct
    19861 ggcgtcttcg gcttcccagt tgcgatagcc ccagtagtgg cccagaccgt tgatgacacc
    19921 ggccgcccag aagggaatcc acacgatctg cgtgaggaac accagggcac cggggatgag
    19981 gccgaacaag gccagatcga tgagcgccat gatggtgagg ccggttttca catagcgtga
    20041 atagaccttg cgctcgagcc agtcgtccgg cgtgccatgg ccgtagcgct ccatcgtctc
    20101 cttgttgtac gactccttga cgtagaggaa cacgccgccc cagagcacgc ggttgatacc
    20161 cacctgttgc gggctgtgcg gatcctcggg cgtttcgcat ttggcgtggt gcttgcggtg
    20221 gattgccgcc cactccttgg tgaccatgcc ggtcgtaagc cacaaccaga aacggaaaaa
    20281 gtgcgatgcg atcggatgca gttccagcgc gcggtgcgcc tggcagcggt gtaggaagat
    20341 ggtgaccgaa gcgatggtga tgtgcgtcag gacgagcgca acgatcatca cgccccacca
    20401 gggcagatcg aacacaccgg aataaagcat ggcggacaat gggtttcctt ctaatgttga
    20461 ggacagctcc ggaattctac ggcaatttgg cgaatcccac ggattgtcgg tgaactgccc
    20521 ggagcgcgcg gtcagaccgc ctcccgcggc taaagttgca gggtctacgc gggcttgcga
    20581 ggaatcaggc cgccgatttg cgcaccagcg ctgccgcgta aaagccgtcg gtgccgtgca
    20641 aatttggcac cagacgcaaa aatccgtcgg cgtccggctg cgccggcacc tcctccaact
    20701 gggattccag caagactgac gcccggacca gttcgaacga cggatgcgtc gccaggaagt
    20761 cggcgacgat ttcctcattc tcttcgggaa gaaaactgca ggtcgcgtat accagcctgc
    20821 cgccggcttt gacgagcctg gcggcggccg cgaggattga ggcctgcttg atccgcaatt
    20881 ccgccagcgc cctttcgtcc tgacgccact tcaaatcggg attgcgtcgc agcgtaccca
    20941 ggccgctaca gggtgcatcg accagcacgc gatcgagttt gccggcgagt cgccccaggc
    21001 gtgcgtcatg ttcgctcgta atatgccgcg catcgacatt ggacgcaccc gagcgcgcaa
    21061 gccgcggctt cagccgcgca agtcgcttct ccgccacatc gaaggcatag aggcggccgg
    21121 tcgagcgcat cagcgccgcg agcgccagcg tcttcccgcc cgcaccggcg cagaagtcgg
    21181 ccaccatttc gccgcgccgc gcgccgagca gatggcacag caactggctg ccttcgtcct
    21241 gcacttcgat cgtgccgtcg aggaacaggg catgacgctg gagcgcgggc ttgtccagca
    21301 ggcgcagacc ggtgggcgca tacggcgtcg gtgcgacgcg cagaccctcg gccgccagtt
    21361 gccgctgcac cgcatcgcgc gtgctcttca gcaggttgac gcgcagatcg agcggcgccg
    21421 gctgctgcag tccttcggca taggcgagga tgcgctcgtc ggggaggtgg gcgcgcaggc
    21481 gcgcgatcac ccagtccggc aggtcgcagc gtacttcgaa gggcagctcc ccgcgcgcgg
    21541 ccgccttgac acgctgcagc caagcgccct cttcgctgcg ctcgctgagg cccagctcgc
    21601 gcagattgac gccgcgcaca cgcgccagcg tgatgagcgc aaacgcacgc gccgtcgcta
    21661 ccggagtcag ccgggcttgc ggcttgacgg gtccgacctc ggcgaggatg taatcgacca
    21721 gccgcttgcg ccgcaacccg gcataaaccg tttcggccag aaatccgcgc tcgccctgcc
    21781 cgagcttggg atgcgcacgg aaataatccg acaggacctt gtcggccgca cgctcgaaac
    21841 gcagcgcgcg cgccgtgagg tcggcgagcg tctccagcaa agcaggggtg atgcgcacgg
    21901 ccatgcgcta cgcgtcgcta tagacgagtt gcggttcgtt gcccgccacc cgcaccacgc
    21961 cgttctcgac gcgcagcttg tcttccagga accagcggat ggcgcgcgga tagatcacat
    22021 gctcctggcg cagcaccctc gccgcgaggc gctcgggcgt gtcgtcgggt cgcaccggca
    22081 ccgccgcctg gatgatgatc ggtccttcgt ccagcgtggg cgtcacccag tgcaccgtgc
    22141 agccgtgcag cttcacgccc gcggccaatg cacgcgcatg cgtgtcgagt ccggtgaagg
    22201 ccggcagcag cgcgggatgg atgttgatca tgcggccggc gtaacgctcg acgaaggcat
    22261 cggtaaggat gcgcatgaag cctgccagga cgacgagatc gggcgcgtgc cggtcgatct
    22321 cggccgcgag ttgcgcgtcg aaggcttcgc gcgtcggata gtccttgtgc gcgacgaccg
    22381 cggtcggaat gccgcgcgaa cgcgcataag ccagtcccgc cgcatcggcg cgattgctga
    22441 tcaccgcgac gacggggagg tttgcttcga ccaccgcctg catattgctg ccgcggccgg
    22501 aaatgagtac gacgattttc ttctgcatgc agcgcgccgt gcggccctga aagcgaagcg
    22561 ccgcagtccc gcggcgcacg gggcgcattg taccgtcagg cggcagcgac tgcccttaag
    22621 cgtcttcccg cgcagcctcg aacgcgcgca ccatgcgcac gagttgccgc gccacgcctt
    22681 ccaccgacgc gtgcacgcgc ggatccagca acgcgccgtc ggcatcgaag gcttcgtgcg
    22741 cgcgcgccag cgcatactgc ttcgtctgca ccagcacacc gaggctttgc agtatggcgc
    22801 gcaactgcgt caggccgcgc aagccgccga tgttgccggg cgaggcggcc agaagcgcgg
    22861 cgaccttgtt ctggtaaggc accaggccgt tctgcccgct gtccggtcgc gacacccagt
    22921 cgagcgtgtt cttgagcagc gccgacaccg aggcattgtg ttcgggcgag gcgatgagca
    22981 accccgcgtg cgcgacgaag attgctttca gcttgcgcgc attggccggc aggccttcgc
    23041 gcgcttccag ttcgccggta tagagcggca gcggatagtc ggccagatcg agcacggtca
    23101 cttgcgcacc ggctgcctcc gccgcgcgca ccgccacctc gagcaaccgg cggttgagcg
    23161 aaggccggct gccgctgccc gcgaaggcga gaattctcga attctgcatg cttgtctcct
    23221 gagacggccc cgcttacgcg ggcgctgtcg ttctggatat ttgaataaac cggcgcgata
    23281 ctaccccggc cgtcacggtg cccatagcga ataaattcca tcaacttcat cgaatgaacc
    23341 gaatgaaatt gagcctcgaa gcgctgcagg tgctggacgc catcgaccgt aagggcagct
    23401 ttgccggcgc agcggcggaa ctgcaccgcg tgccctcggc actgacctac caggtgcaga
    23461 agctggaaca ggatctcgac gtgctgctgt tcgaccggcg cggtcatcgc gccgtactga
    23521 ccgcgccggg acgcgaacta ctcaaggaag gccgcaacct gctgcgcgcc gccggcgacc
    23581 tggaaagccg cgtcaagcga ctggccaccg gctgggaggt ggaactgcgc ctgacgctcg
    23641 ataccagcct gtcgttgcag ggcctgctcg atctcgtggc ggctttttat cgcgaggatg
    23701 ccggcacgcg cctgcgcatc ggccacgaag tgctgggcgg cacctgggat gcgctggtgg
    23761 atcgccgtgc cgatctggtg atcggcgcat cgggcgaagc ccccacaggc ggcggctaca
    23821 gttcgcagcc gttgggcgaa gtggaatggc tgttcgcggt tgcgccgcat cacccgctcg
    23881 cccgcgaagc ggaaccgctc gaacgcgaca ccatcctgcg tcatcgcgcc gtggcggtgg
    23941 gcgacacttc gcgccggctg ccgccgcgca gcacgggtct ctacaacggt caggacgtgc
    24001 tgaccgtgcc cgacatgcag gccaaactgc aggcgcaact caccggactc ggctgcggct
    24061 tcctcccacg cctgctcgcg caaccgctga tcgatgccgg ccgactggtg gcgaaggaag
    24121 tggaggaaag caagccctcg ggcatgctgc gctacgcctg gcgcaccggc gaaacgggta
    24181 aggcgctcaa atggtttctc aagcggctgg agcaggccga gacgcgacgc gcgttactgg
    24241 gatgctgaac accgacgtct atcgcggccg gttcgcgccc tcacccaccg gtccgctgca
    24301 tttcggttcg ctggtcgccg cggtgggcag cttcgtcgat gcgcgtgcgc acggcggtac
    24361 ctggctcgtg cgcatggaag acctcgatcc gccgcgcgtc gtgcgcggtg cggccgacaa
    24421 catcctgcgc acgctcgaag cgcatgcact gcactgggac ggcgacatcg tgtaccagag
    24481 cacgcggcac gacgcctatt cggccgcctt ggagcagctt gccgcgaagg gcgcgacctt
    24541 ttcttgcgcc tgcacgcgga aggaaatcgc cgattcggca ctgggaccgg acggcgccgc
    24601 ggtctatccg gggacctgtc gcaacggtct gccgcccggt cgcagcggtc gggcggtacg
    24661 cgtgcgcgtg gacgacacgc ccatcgtctt ccacgaccgg ctgcagggca accagcgcca
    24721 cgatctggcg cacgaagtgg gcgacttcat cgtgcgacgc gccgacggcg tccatgccta
    24781 tcagcttgcc gtcgcggtgg acgacgcgta ccaaggcata acggatatcg tgcgcggagc
    24841 cgacctgctc gcctccacgc cacgtcagat ccatctgcaa cggctgctcg ggttcgccac
    24901 accgcgctat ctgcacctgc ccgtggccac caatgcacgg ggcgagaagc tttccaagca
    24961 gacgcttgcc gcaccggtcg acgcagagta tgcaagcgca ggactcattg ccgcgctcgg
    25021 cttcctcgat caccggccgc ctgtcgcatt acacggcgcg ccgccggagg aactgctgca
    25081 atgggccgtt gcgaactggc aggtcgcgcg cctgccgcgc acgcgccagt tgccgcatcc
    25141 ggtgtccgct tgaagcgaat caggtgcgcg tcggcgcttc gtcctcgcaa gtcgcgccca
    25201 acgcgagcac gccttcacgc agcagcttca tcgctgcttc ggcctgcgcc ggctcaccgc
    25261 gcacgcccag ttcgatgtgc cgccgcacgt ccttgcccac atagggcaga ctgaacactc
    25321 ggacgccggg atagcgcgac tcggcttcga tcatcagcgg cgtggccgcg ctttccggaa
    25381 tgccgtagac ataaaccgac agctcgcgct cggacaggct gtgatgcaga tgcgcgtagt
    25441 gatggtcgag cacccattcg gccatcggat gcgccatgac cgggaagccc ggcatgaagt
    25501 gatggtcgcg aatggaaaag ccggggatgg tgttgtagct gttgggcagc agttgtgcgc
    25561 cctcgggaaa ctcgcccatc tgcaattgct gcggactggc tgcgcggccg taacgacgct
    25621 gcatcgccgc ttcgatcagc gcgatcgcgt ccgcgtgtcg cacgagcttc aggcccagcg
    25681 cctcggccgc ggcctgacgg gtgttgtcgt ccggcgtcga gccgatgccg ccgaagcaga
    25741 acaccgcgcc gccctgcgcg aagctgcgac gcaacacttc gaccagcccc gtgcggtcgt
    25801 caccgacgta ttccgcccgc gacagataca ggccgcgcgc ggacagcagt tccaccattt
    25861 tggcgaagtg gcgatcggtg cgcttaccgc gcaggatttc gtcgccgata atgagcgcac
    25921 tgaatttcat gcgcgtcgca tcctttccaa ttgcgccagg ccgtagtgga tgaacgccag
    25981 gccggcaatg acaggaatga aaaaaccgac cagcggaatc tgcgcaatca gcgccaggat
    26041 gatcgccagg ccgtacatgg cggcattgtg gcgcttgaac agcaggcgca tctcttccgg
    26101 ggtggcatgc tcggagacgg catcgtaacg gaacatgcgc tggttgaagt agccgagcag
    26161 cagcaccggc agcaccggcc acagcagcgg gatgaaccac aacggcaggg tcaccagact
    26221 gaggagcaca aagaccccca gcgccacgaa cgcattgacc gtcatgccgg cgatgctgcc
    26281 gccctgctcg cgccgcagtt cggggtagta gttgcgcgcc acgtgctcga ccatgatggt
    26341 catgccgaac acgccgacga tgaacgaggc ggtgacgatg accagcggca cgaacaggat
    26401 gaggagcgcc gcccagccca gcaccgccgc caccggctcg aagggcaccc attgcgagac
    26461 ccattgcgcg accgccgagg tggcgatctg accttccagc caggccaccg tctgcgcacc
    26521 gaacagcagg gcgatcacgc cccagaatag cagcgcgagc gccagcggcc agatcatcag
    26581 cagaatcatg cgcgggtgca gcgtgctggc gagcgcacgc accagcgctt tggcgatttc
    26641 ggtcatcgcg tactcggctt cggcatcatc gggatgcgga gcgcgcagtg tgccacagcc
    26701 gccctcatcg ccgcagcgcc tcccacgcct tttgcagccg ctttaccgac acgggcgccg
    26761 gcgtgcgcag ttcctgggcg tagagctgca cgcgcaactc ctccagcatc caccggtact
    26821 gttcgagctg cggatcggct tcgccctgct tgcgccgctt ggcgtcttcg cgctgccatt
    26881 gcgcctgcag cggcgcgtat tcgctcatcg cgcgcgcgtc gcgggcggga tcggtgcgca
    26941 gcttgtccag gcgcaaggca atggccttca ggtagcgcgg gaaatgctgc agccgctcgt
    27001 agggcgtgtc ctcgatgaag ttcttgggca gcaggcgcgc aagttgatcc tcgatatcgc
    27061 gcaccacctc gggatacgcc ttcagcgttt gcagcttctt cgacacggac tgacgttcgg
    27121 tgaggatcac accgatcaga cgcgccagtt cctgtccgat gagcgacagg cgtgtacggc
    27181 cttcgtcgcg acgctgttcg aactgctcgc gcgtagccgg ccaaggctcg gccatgcagg
    27241 cgcgatcgaa ggtggcggcc agcagttgac gcttgaggtc ggccgcttcg ccaccgagcg
    27301 cggcatactg cagtgccatg gtctggaagc cgggaaagtt cttctccaga tacttcgcct
    27361 gttccttcaa ctgcagcata aaaagccggc gcagaccggc gcgatgcgtc gcgcgtgcct
    27421 tgtcctgcgc gtcgaatact tgcaggctga cgctgtcgcc ttcatcgacg agcgccggat
    27481 agccgatcag gatctgcgat ccgctgcgga tctccatgac ttcgtcgagt tcgccgaagc
    27541 tccagtcggt caggccgtcc atcgccgccc tgggctgcgc gacggcggcg aactgctcgc
    27601 ccgccttgtg ccccagttcg gcgcgcagcg cggcgaggtt gcgccccatc gccaattggc
    27661 ggccgtgctc gtcgacgacg cggaagttca ttatcagatg cggcggcagc gtgtccggcc
    27721 ggaaggtgtc cagcggtagc accagttgcc gttcgctgcg cgcatagcgc gcgatggctt
    27781 cggccagcgc attatccgaa ggcgcaacgt ccgcgacgaa cgccgcggca aactcctgca
    27841 aggggccgag gcgatgccgc aggcgctgcg gcagcgactt ggccagttgc atcacctttt
    27901 ccttcaacag acccggcacc agccattcgc agcgcgcggc gctcacctgg ttgagcgcga
    27961 tcagcggcac ggtcatcgtc acgccgtcgc gcggtccgcc gggatcgtgc agatactcca
    28021 gcgcgaaact tcggcccgcc atttcgagcg cgggcggaaa ctgcgcagtc gtgatgcccg
    28081 ccgcctcgtg gcgcatcaga tcctcgcgcc ggagaaacag cagtttgggg ttctgcgctt
    28141 ccgcctcctt gcgccaggca tcgaaggccg cgccgttgtg gatgccggcg gggatgaggc
    28201 tgtcgtagaa cgcgtagatc agctcgtcgt ctacgagcac gtcgggtcgg cgcgacttgt
    28261 gttccagcgc ctcgatctgc gccaccagcc ggcggttgtg ctggaagaac ggcgcacgcg
    28321 tgtcgtactc gcccgccacc agcgcctggc ggatgaagat ttcgcgcgag gtttccggat
    28381 cgagcggccc gtagtggatg cgcttgtgcg tgtagatcgg caagccgtac agcgtggcgc
    28441 gttcgaaggc ggccacatgc gcgggcttct tctcccaatg cggctcgtag cgatgccgct
    28501 tgacgagatg cgcgcccacg ctttcgagcc agtccgcttc gatgccggcg acggcgcgtg
    28561 catacagccg cgtcgtttcg gtcaattccg ccgccatgat ccagcgcggc gccttgcgcg
    28621 ccagcgcgga gcccgggtgg atcaggaact tgatgccgcg cgcgcccagg tattgcccgt
    28681 cctcctgctt cagaccggcg ttgccgagca gaccgctcaa cagcgcacga tggatttgcg
    28741 cgtaggtcgc gggcttttcg ttcgggcgcc agccctgttc gctcaccagt tccttcaact
    28801 gcgtatggac atcgcgccat tcgcgcatgc gcaggtacga aagaaaatga tcgcggcagg
    28861 cgcgatgcag cttgcgattg gactggcgat gcagcgtgtc ctcatagaac ttccacagct
    28921 tgaggaagcc gagaaaatcc gatttctcgt cggcgaactt cttgtgcgcc tcgtccgccg
    28981 cctgcgcgcg ctcggccggc cgctcgcgcg gatcctgcac cgaaagcgcc gacgcgatga
    29041 ccagcgcctc ggtcagacat tgttcctgcc gtgcggcgag caccatgcgc gcgatgcgcg
    29101 gatcgagcgg caatctcgcc agttgccggc cgatgtcggt cagctcgcgc gcttcgtcga
    29161 ccgcgccgag ttcctgcaac agcgcataac catcggtgac tgccttgggc gccggcgcat
    29221 cgatgaaggg aaagtcttcc acggcgccga gccgcagcgc cttcatgcgc aagatcaccg
    29281 cggcgagcga cgagcgcagg acctccggat cggtgtagga cggccgcttt tcgtaatcct
    29341 cttccccgta taagcggatg cagacccccg ccgccacgcg gccgcatcga ccggcacgct
    29401 gccgcgcggc cgattgcgct acaggctcga cgcgcaactg ctcgaccttg ttgcggtagc
    29461 tgtagcgctt gatgcgcgcc agcccggtat cgaccacgta gcggatgccg ggcacggtga
    29521 gcgaggtctc ggcaacgttg gtggccagca cgatgcgccg ccccgcgtgg cccttgaaca
    29581 cgcgctcctg ctcggccgcc gaaagccgcg catagagcgg caggatttcc gtgtgcggcg
    29641 gatgatgctt gcgcagcgct tcggccgctt cgcgtatctc ccgctcgccc ggcaggaata
    29701 ccagcacgtc gcccggcccc agatgtgcca gttcgtccac cgcgtcgctg atcgcttcgt
    29761 tgatgtcgat ttcgccgtcc tcgttttcct gcggcgggcg atagcggatt tccacggggt
    29821 agagccggcc cgacacttcg atgaccggcg cgggttcgcc gttcgcttgc gcgaagtgtc
    29881 tggcgaaacg gtcggcatcc agcgtcgcgg acgtgatgat cactttgaga tccgggcgcc
    29941 gcggcagcag ttgtttgaga tagccgagca ggaaatcgat gttgaggctg cgttcgtgcg
    30001 cctcgtcgat gatgagcgtg tcgtaggcgc gcagttccgg atcggcctgc gtttcggcaa
    30061 gcaggatgcc gtcggtcatc agcttgatgt agctggacgg actggtgcga tcgttgaaac
    30121 ggatcttgta gccgaccgcc tggccgagtt cgctttgcag ttcctgcgca atgcggcttg
    30181 ccgtggcgcg cgccgccagg cggcgcggct gggtgtggcc gatatagccg tgcacgccgc
    30241 gaccgatgga cagacagatc ttcggcagtt gcgtggtctt gcccgaaccc gtttcgcccg
    30301 agacgatgac gacctgatgc gcctcgatgg cgcgagcgat ttcctcgcgc cgcgcgctga
    30361 cgggcaactc ctccgggaaa ctcgggcgcg gcagattcgc gcgccgcgcc tcgatcactt
    30421 ccggtcgcag gctgcgcgga cgatcgttgg ggcgggaacg gcgtttgaga ggtgcgggag
    30481 cggacatcgg gcgtcgtgcg gcctaagcgg ccataatgcg gcgtccgcgc gcggcgtgag
    30541 tcatgaatcc ggatcgaaag tagcgcaaat gaatatcctg ctctgcggcg ccaacggttt
    30601 catcggtcgt catctggcgg ccgcgctgga agggcgcgga catcgcgtcg tgcgcggcgt
    30661 acgcgacacg gaagacggca aggacgctgt ggcgatcgat tacacgcgcg atcacggccc
    30721 tgatatctgg cgtccgcgac tggaaggcat cgacgtcgtc atcaacgcgg tcggtatttt
    30781 acgcgaacgc ggcacggcga ccttcggtgc cctgcatgcg cgcgcgccga tcgcgctgtt
    30841 cgatgccgcc gcacaggtcg gcgtgcgccg cgtggtgcag atttccgcgc tcggcgccga
    30901 cgaaggtgcg caaagccgct atcaccgctc caagaagcag gccgacgatt atctggccac
    30961 gctgccgctc gactgggtcg tcgtgcagcc ttcgctggtc tatggaccgg gcgggacgag
    31021 cgcgaagctc ttcgacacgc ttgccgcgct gcccctcatt cctctgccgg gcggcggccg
    31081 gcaacgcatc cagccggtgc atatcgacga tctcgtcgaa gcgatcgtgc ggttggtgga
    31141 atacgacggc cccttacgcg gaatctttcc cgccgtcggc gcggaggcgc tgagcttcgg
    31201 cgaatggctg cgggcagtgc gtgcgcaatt gggtctgccg cacacgctcg cgctgcccat
    31261 tcccgcctgg atgatgcgca tcgcagcagc gctcggcgag cgtctgcccg gcctgctcga
    31321 ccgcgaatca ttgacgatgc tggaacgcgg caacacggcg cccagcgatg ccatgaccac
    31381 gctgctcgaa cggccgccgc gtccggtgac gacattcatc gcaccggaac gcgcacgcga
    31441 gacggccttg ctcgcccgac tggcatgggc actgccgctc ctgcgcttca gcgtcgcggc
    31501 cgtgtggatc ctgaccggtc tggtatcgct ggggctttat cctgccgcgt cgagctatgc
    31561 tttgctggcg caggtcggcg tcacgggcgc gctcgccccg gtgacgctct acggcgccgc
    31621 gacgctggat ctcgccttcg gcatcggcgt ctatgccctg cacccgccgc gacgccgctg
    31681 gctgtggcgc gcacaaatgc tgctgatcgt cggctacagc ctgatcatcg cgtggcgttt
    31741 gccggaatac tggctgcatc ccttcggacc gctgctgaag aacattcccc tgcttgccgt
    31801 catcgtgctg ctgcacgaat tcgacgacac ggatgagcat tgatggaata cctcatcgtc
    31861 aaatggctgc acattctgtc ctcgaccctg ctgttcggca cgggactcgg ctcggccttc
    31921 tacatgtatt tcactaatcg cacgcgcaac gtcgaagcga tggccatcgt cacgcgcaag
    31981 gtggtcgcgg ccgacttcct gttcaccacg cccacggcca tcctgcagcc gttgaccggc
    32041 ttctggctgg tgcaactggc gggctttccg ctgacggcgc cgtggattct gtggtcggtc
    32101 gtgctttacg tcatcgccgg cgcgtgctgg ctgccggtgg tgtggatcca acttcggatg
    32161 gcgaaacttg cagaggcggc cgccgctgcg aggcagcctg tgtcggcgga gtactggcgc
    32221 ctgcatgccg tctgggtatt gctcggcgtg cccgccttca tcgctttcat cggcattttt
    32281 tatctcatgg tggtgaaagc cccgcccttt tgagcgcgcg cctgcgcggc ggcagccagt
    32341 cccagcgcga gcgcggcata gacatagaac agcgaacctc cctccaggcg cgcttcgaaa
    32401 cccggcacga agcgctcggg cactacatgg tagtcgacga cataagccgt gacggcagtc
    32461 gccgcgccgc cgaccaacgc atccggaacg ctgcgttcgg cgcgccggcc gaacagcgct
    32521 tcgaaaccga gcgcccagaa gatcgatgcg ccgtggtgta gcgcgaagcc cacggcggta
    32581 ttcacgccgt cgtgccgcgc ctgggatctc gcactgccgc cccaggcaat atggctgacg
    32641 gcgttcaacg gcccggcggc gtgccccgcc ttgcgcccgc accaggacgc ggccacggcc
    32701 gaggtcagcg cagcgaccgt cccggagacg agcgcacgcg gcagcagatc ctgcgcgccc
    32761 atgttcaggc gccgggcggc gcggccatga ggaaatcctc gtgctccagc gtctgcttca
    32821 ggcgttgcag atcctcgtgc aaatcccgct gcggatcgcc cagcgcctga cgtgccattt
    32881 cgccgcgcag gccggcgggc agcatatagc gcatctccac cgtcaccttc gtggcgagcg
    32941 catcattcgg cacatcctcg aaacgcacca cgccctcgtg ccgcaccggg ctgtcgacgc
    33001 tgcgccaata gatcgccttg cccggatcgt cgtcggtgat ttcactcacc caggaaacga
    33061 cctgtccctg cgcagtccgc acggtccagc gcgagcgacg ctgatccagt tgctccaccc
    33121 gctccacgcg tttcatgatc gacggcaggt tttgcacgtt gcgccagaag cggtaagtgg
    33181 aatcgcgccc cgcgccgacc tgcacgctgt cggagacaag catcggttcc aaggccgcac
    33241 cggccaccga ttcggcaaag ggaatgcgcc ccgtccaggc gtggtagagc aaacccgcac
    33301 ccgcgag
//
LOCUS       DATGOU010000128        14935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_1731459, whole genome shotgun sequence.
ACCESSION   DATGOU010000128 DATGOU010000000
VERSION     DATGOU010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14935
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1731459"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VKZ48_07585"
     CDS             complement(<1..212)
                     /locus_tag="VKZ48_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HLU77747.1"
                     /translation="MKKIEAVVKPFKLDEVREALAEVGVTGLTVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEVVLTDEMLG"
     gene            328..1434
                     /locus_tag="VKZ48_07590"
     CDS             328..1434
                     /locus_tag="VKZ48_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLU77748.1"
                     /translation="MDAAMWNALAGEHPFLRHEFLDALHDTGCAAPDTGWTAQYLTAW
                     RREALVGALPLYVKTHSYGEYVFDWAWADAYERCGLHYYPKLLSAVPFSPVTGPRLLG
                     EDEEARAALRRTALQLAQQLSSLHVLFPDVREAEGWRDAGCLLRRGVQFHWRNEAYAS
                     FDQFLGTLNRAKRKNIRQERRRVAEAGVRFKRKTGREISEADWRFFTRCYDNTYREHY
                     STPYLSFDFFVRLGETLPDNVLLVIAEREDRPIAAALDLYTPQTLWGRYWGALESVPG
                     LHFETCYYQALEFCIERGIALFEGGAQGEHKLARGFMPVKTWSAHWLKHPQFADAVAR
                     YLEREAVGVGGYIDELNERTPFKGSGESTVKSRE"
     gene            complement(1480..2028)
                     /gene="ppa"
                     /locus_tag="VKZ48_07595"
     CDS             complement(1480..2028)
                     /gene="ppa"
                     /locus_tag="VKZ48_07595"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HLU77749.1"
                     /translation="MNLDRVDSGRDVPNDFNVIIEIPAHADPIKYEVDKETGAMFVDR
                     FLSTPMRYPCNYGYVPRTLSGDGDPVDVLVITPFPLCPGVVVRCRPIGMLKMEDEAGE
                     DTKVVAVPVDKLTTFYHNIRTVHDLPEERLAHITHFFEHYKDLERGKWVKVVGWFGPE
                     EAKKDIQDGVDTYARARKKPAF"
     gene            complement(2165..3553)
                     /locus_tag="VKZ48_07600"
     CDS             complement(2165..3553)
                     /locus_tag="VKZ48_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006389016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLU77750.1"
                     /translation="MTTFTELGLIPELLRAVAEQGYNEPTPIQAQAIPLVLAGRDVMG
                     GAQTGTGKTASFALPLLQRLAPHANTSASPARHPVRALILAPTRELAAQVEESIRSYA
                     KYLPLRSACIYGGVPMDPQIAQLRAGVEVLVATPGRLLDHVQQRTLNLSQVEIVVLDE
                     ADRMLDMGFVPDVRRILALLTNRKQGLLFSATFSDEIKKLADSFLKQPTLVEVARRNQ
                     TAESVSHLVHPVAREKKRELLVHLIKSRDMKQVLVFCSTRLGTNRLAYQLAREGVQAQ
                     AIHGDKTQSERTQALEEFKSGKDGVLVATDVAARGLDIAELPYVVNFDLPNSPEDYVH
                     RIGRTGRAGATGTAISLVSADEHERLEAIEKMIKQRIPREIVPGFGPHPADAPPMMEP
                     RRDRQRGADAARLTGAKHAPRGKQQAPMDPIFNTPYEPGLYTPPAARAESPRKPKKQV
                     AALLGGLVRKPG"
     gene            3740..5635
                     /locus_tag="VKZ48_07605"
     CDS             3740..5635
                     /locus_tag="VKZ48_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLU77751.1"
                     /translation="MITLSRLSLRRGAKLLLDQADAVIHPGQRVGIVGPNGCGKSSLF
                     ALLRGDLHADGGDVAVPPGWVIAHVAQETPAAERSAIDYTMDGDGELRAVEAEIADAE
                     AQDDGERLAHAHERYREIDGYAARARAAALLDGLGFAPDDIERSVREFSGGWRMRLNL
                     AQALMCRSDLLLLDEPTNHLDLDAVLWLEGWLQSYRGTLLMISHDREFLDGTISAVLH
                     FDRQKLKRYSGNYSAFEEQRAAQLAQQQALHARQQSEIARLERFVERFRAKATKARQA
                     QSRMKALERMERIAPAHVDSPFHIRFREFPGGPDPMLTLEDASAGYGDTPVLRDIRFT
                     IPAGARIGLLGRNGAGKSTLIKLLAGRLAPQSGKRREGKDLRIGYFAQHQLEDLRADE
                     TPLQHLVRLDSATREQELRDYLGGFGFAGDMALAKVAGFSGGERARLALALIVWQRPN
                     LLLLDEPTNHLDLDMREALTVALQEYEGAMVLVSHDRHLLRTACDTLMLVADGAVSPF
                     DGDLEDYRDWLAQRQPRDDGRAKSTAGDQRREQKRAEAEARNRLAQARKPIEKRLRAV
                     ESAMAKLNTDKARLEAMLAAEDFYTRSTPEQVTAALREQAQVAHELEQAETEWFALQE
                     ELEALSA"
     gene            complement(5658..6182)
                     /locus_tag="VKZ48_07610"
     CDS             complement(5658..6182)
                     /locus_tag="VKZ48_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77752.1"
                     /translation="MRLHQLKLEYNVEQDRLLLRLATDQNAEVLFWLTRRVVLRLWSV
                     LLDVAHAIPEIILQPNPEARRALLGFRHAVAVSEAEFNQPYAEVARSYPMGMEPLLVA
                     RVDARKGDKGQHVLNLHPANGESVHINLDEKLVHGMLKLIENGVAKSDWDLTLRVAST
                     SVPVYANGDHATIN"
     gene            complement(6349..8955)
                     /locus_tag="VKZ48_07615"
     CDS             complement(6349..8955)
                     /locus_tag="VKZ48_07615"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HLU77753.1"
                     /translation="MFAKALATTVLFLALATPAVEAAPVSYEGTIAPGTTVGGSVGGF
                     SWGDEDGAGVDYWRFSGSVGDVITLEGLRIDPALDPAFSLYFGITDADDSEFLADADW
                     GGLQFLTFADDEIDNDGPGGDPLLEGFVLPFSGDYTVVLGGFLSDDEGPYAYSLSLAV
                     EPTGVPEPGTALLLLGGIGALLWHRRKRVPQSTTAIAVAILGTALIAAPAEARITRID
                     VTSVESPTFEGRTFGSVGAYEKLRGIAYGELDPGDPRNAVITDIELAPLNANDRVEYS
                     MDFYILKPINLAQGNHKMFMEVNNRGGKLFGGFNLSGGGNNPTTAEHAGEAFLMNRGY
                     TLVWNGWDPSAPPGGDRLTINVPVATNPDGSTITGPSYEYIVFDNPTSLTYGLSYAAA
                     TLDKGEATLTVREHLNDTPVTVPADGWEYVDERTIRLLPEGTPFQQSHIYEFTYTAKD
                     PLVTGIGLAATRDFVSFLRNANVDDFGNPNPLAGDIQYTLAFTISQPGRYMNDFQTLG
                     FNEAEEGGRVFDGVENWIAGGSGDAINFRFSQTARTERNRQNHLYPENLFPFAWPVLS
                     DPLSGKTAGRIERCAATDTCPKVFEVNSSNEYWVKSGSLLHTDLQGNDLKDPDNVRFF
                     LLSGVEHTVNGSPPNSPGICQQPRNTTNPNPALRALFIALDEWVTQNKRPPKSDVPSA
                     SKKKTAAFAVPVPGSQVGFVPQSELGWPNIPGVKYTGVISTRYRLDFGPMFDDGILTN
                     YPPSVVGRPTYAHFVSKVDKDGNEVAGIRLPPVAAPIATTTGWALRAPEFGGDDGCES
                     SGQTIPFKRTKAERLAAGDPRLSLQERYKNHAGYVKAVEKVARKLEKQRLLLPEDVAR
                     YVEQAQQSDVLK"
     gene            complement(9102..9620)
                     /locus_tag="VKZ48_07620"
     CDS             complement(9102..9620)
                     /locus_tag="VKZ48_07620"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HLU77754.1"
                     /translation="MDVSYYIAASLDGYIAAADGGTAWLKPFETAHEDYGYAAFYAGI
                     DAVLMGSCTFEQCLRYERWPYADKPCWVFSSRTFERVPPGVTVLNQSPAQALAALESR
                     WLKRAWLVGGGKLAASFAAQGLITEYVISYLPILLGEGVPLLDGASLREELTLVDAKR
                     HTSGVMQLRYTA"
     gene            complement(9629..10699)
                     /gene="queG"
                     /locus_tag="VKZ48_07625"
     CDS             complement(9629..10699)
                     /gene="queG"
                     /locus_tag="VKZ48_07625"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020655634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HLU77755.1"
                     /translation="MSTTEQLVERDWPALALAVKAWGRELGFDAVTIADVNLGDAEPR
                     LLHWLEQGMHGEMDYMARHGLKRARPGELVPGTIRVISARMGYWPDAADSDAALADAD
                     RAYVSRYALGRDYHKVLRARLQQLADRIAAEVGEFGYRVFSDSAPVMEVELASRSGLG
                     WRGKHSLLLTREAGSFFFLGELFTDLPLPVDVPISAHCGTCTRCIEVCPTQAIVAPYV
                     VDARRCISYLTIELKGSIPLELRPLIGNRVYGCDDCQLHCPWNKFAQRTREADFAVRH
                     GLDRATLVELFGWSEDEFRQRMAGSAILRIGYERWLRNIAVGLGNAPTSNAVLTALRA
                     RADHSSELVREHVAWALARHEA"
     gene            10743..11213
                     /gene="tsaE"
                     /locus_tag="VKZ48_07630"
     CDS             10743..11213
                     /gene="tsaE"
                     /locus_tag="VKZ48_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005016487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HLU77756.1"
                     /translation="MVAPLALHLSDEAATDALGARLARALTPGLSIHLVGDLGAGKTA
                     LVRACLRALGHTGKVKSPTYTLVEPYAVSKLPLYHFDFYRLREPTEWLDAGFRDYFNA
                     DSVCLVEWPEKAAELPPPDLRISLRHAPSGRDATLTAYSPQGERLLQSISSAAG"
     gene            11240..12427
                     /locus_tag="VKZ48_07635"
     CDS             11240..12427
                     /locus_tag="VKZ48_07635"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HLU77757.1"
                     /translation="MVAVVPFEVAHATSGIRSVRMWPAQDYTRLTIESTQSLKHRLIT
                     LSNPDRVVIDIEGADLQALQREAANKVLAHDPYVMQMRVGQFTPEVVRLVIDLKEGVK
                     PQLFTLAPIGDYGHRLVLDLYPINPPDPLMALLREQAERDAVRGPAPLPQRTITIVVD
                     AGHGGEDPGAIGARGSKEKDVTLKVARKLKQLIDAEPNMRAVLTRDGDYFVPLHMRVA
                     KARQVRADLFVSVHADAFIKPHARGSSVFALSERGATSAAARWLANRENESDLIGGVN
                     LDVKDRYLAQTLLDLSLTATISDSLKLGKLVLTELGGINKLHKEYVEQAGFAVLKAPD
                     IPSILIETAFISNPEEERRLNDNAYQDKMARAIVTGIRNYVQKYPLGGPSTLAARTKQ
                     PDA"
     gene            complement(12424..13083)
                     /locus_tag="VKZ48_07640"
     CDS             complement(12424..13083)
                     /locus_tag="VKZ48_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HLU77758.1"
                     /translation="MDILLAFWDLLVRLDIHLASLVQQYGHWIYAILFAIIFCETGLV
                     VTPFLPGDSLLFVAGTIWAAAGMDVMLLAALLVIAAVLGDTVNYSIGKWCGPRVFQWD
                     DARIFNKKALDRTYAFYEKHGGKTIIIARFVPLVRTFAPFVAGIGRMAYRRFIAYNVI
                     GALLWVVLLVYAGYFFGNIPFIKNNLTLVIFAIIGLSLMPMAVEYLRARSRNARLRAE
                     S"
     gene            13175..>14935
                     /gene="mutL"
                     /locus_tag="VKZ48_07645"
     CDS             13175..>14935
                     /gene="mutL"
                     /locus_tag="VKZ48_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012078327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HLU77759.1"
                     /translation="MSKIRPLPDTLISQIAAGEVVERPASVLKELMENSLDAGATSIA
                     VSLSEGGGKHIRVTDDGGGIDAEDLSYALARHATSKIATLNDLERVATLGFRGEALAS
                     IGSVARVSVTSRTADAPHAFRIRAEGGPPGAVEPAAHAVGTTVEVNDLYFNTPARRKF
                     MRKEATEFAHCNDVFQRMALARPDVAFSLKHNGRVSAHLPAADLAARAAAVLGADMQE
                     AARLVDAEVPGIALRGFAGSPAHARGNRDAQYIYVNGRFVRDKVLVHALREAYADVLH
                     GDRHASYLLFLEIDPGAVDVNVHPAKTEVRFRDSRAVHQFVYHAVSRALAGSVAAAPR
                     PVADPLEHVSPHPEAPPLYRRQDGLAVAQPVAAYTSMFADASAADAPAPAPRPAPAQS
                     AEHDSAAPPLGYALAQLHGIYILAQNATGLVLVDMHAAHERILYEKLKAALDQHKITG
                     QQLLIPVTFHAEALDVATAEEERETLAQLGFDIAAVSPTALAVRAVPDMLANGDVPAL
                     ARALLQDIREYGASQVLNERQNELLGTMACHGAVRARRALSVPEMNALLRDMEATERS
                     GQCNHGRPTWYQMSLTDLDRL"
BASE COUNT         2731 a         4877 c         4722 g         2605 t
ORIGIN      
        1 ccgagcattt cgtcggtgag gacgacttcg attttcacct tgggcaggaa gtcgaccacg
       61 tactccgccc cgcgatagag ttcggtatgg cctttttgac gtccgaagcc ctttacctcg
      121 gtgacggtga gtccggtgac accgacttcg gccagtgctt cgcgcacttc gtccagtttg
      181 aacggcttga ccaccgcttc gatctttttc atgtggccct cgctgatgaa atccttagca
      241 tcgcagtata aacgcgatca cgcgtcggcg acgcacgcgg ccggcgacgc accggtggaa
      301 atccgactgt gtgaagatct cggcacgatg gacgccgcga tgtggaacgc gctcgctggc
      361 gagcacccct tccttcgcca tgaattcctc gatgcgctcc acgacaccgg ctgcgccgcg
      421 cccgacaccg gttggacggc gcaatatctc accgcctggc gacgcgaagc gctggtcggg
      481 gcgctgccgc tgtacgtcaa gacgcactcc tatggcgagt acgtgttcga ctgggcatgg
      541 gcggatgcct acgagcgctg cggtctgcat tactacccga agctgctgtc cgccgtaccc
      601 ttttcgcccg tcaccggccc tcgcctgctc ggcgaggatg aagaggcgcg cgcggcattg
      661 cgccgcacgg cgctgcagtt ggcacaacag ctctcgtcgc tgcatgtgct tttccccgat
      721 gtgcgcgaag ccgaaggctg gcgcgacgcc ggctgtctgc tgcgccgggg cgtacagttt
      781 cattggcgca acgaggctta tgcgagcttc gaccaattcc tcggcaccct gaaccgtgcc
      841 aagcgcaaga acatccgcca ggagcgccgc cgcgtcgccg aagccggcgt ccgtttcaag
      901 cgcaagacgg gacgcgaaat cagcgaagcc gactggcgct tcttcacgcg ctgctacgac
      961 aacacctatc gcgagcacta ttcgacgcct tatctcagct tcgacttctt cgtacggctg
     1021 ggagagactc tgcccgataa cgtgctgctg gtgatcgccg aacgcgagga ccgtcccatc
     1081 gccgcggcgc tggatctgta tacgccgcag acgctatggg gtcgctattg gggcgcgctg
     1141 gaaagcgttc ccgggctgca tttcgagacc tgctattacc aggcgctgga gttctgtatc
     1201 gaacgcggca tcgcgctgtt cgaaggcggt gcgcaaggcg aacacaagct ggcgcgcggc
     1261 ttcatgccgg tgaagacctg gtccgcacac tggctgaagc atccgcaatt cgccgacgcg
     1321 gtggcgcgct acctcgagcg ggaagcggtg ggcgtgggcg gctatatcga cgagttaaac
     1381 gagcggacgc cgtttaaagg cagtggagag tcgacagtaa agagccgaga gtaacgactg
     1441 ctctccactc tcgggactct ttactctcca cgaatggaat cagaacgccg gcttcttccg
     1501 cgcgcgcgcg taggtatcga cgccgtcctg gatgtccttc ttggcttcct cggggccgaa
     1561 ccagccgacc accttcaccc atttgccgcg ctcgaggtcc ttgtagtgct cgaagaaatg
     1621 ggtgatgtgc gccaaccgct cttccggcag gtcatgcacg gtgcggatgt tgtgatagaa
     1681 ggtcgtgagc ttgtccaccg gcacggcaac gaccttggtg tcctcgcccg cctcatcttc
     1741 cattttcagc atgccgatgg ggcggcagcg caccaccacg cccgggcaga gcgggaaagg
     1801 ggtgatgacc agcacatcga cgggatcgcc gtcgcccgac agcgtgcgcg gcacatagcc
     1861 gtagttgcag ggatagcgca tcggcgtgga caggaagcga tcgacaaaca tcgcgccggt
     1921 ctccttgtcc acttcgtatt tgatcggatc ggcatgcgcc gggatttcga taatgacgtt
     1981 gaagtcgttg gggacgtcgc gtccggaatc gactcggtcg aggttcatgt aatgctgctt
     2041 tttgtggtga tgggttgata gaggtgccgc caagaggcaa gcgatgcgac atgcgtcgca
     2101 atccaagcct gccgtggggc gcgattatac ggttccgcct gcagtacgcg ttgccctccg
     2161 gggatcaacc gggcttgcgc accaggccgc ccagcagcgc cgccacctgt ttcttgggct
     2221 tgcgcggcga ttcggcgcgt gccgccgggg gcgtatagag gccgggttcg tagggcgtgt
     2281 tgaaaatcgg atccatgggc gcttgctgct tgccgcgggg cgcatgcttc gcgccggtca
     2341 gtcgcgccgc gtccgcaccg cgctgacgat cgcgacgcgg ctccatcatc ggcggcgcat
     2401 cagccggatg cggtccgaaa ccgggcacga tctcgcgcgg aatgcgctgc ttgatcattt
     2461 tctcgatggc ttcgaggcgc tcgtgttcgt cggccgagac cagcgagatg gcggtgccgg
     2521 tggcgcccgc acggccggtg cgaccgatgc ggtgcacata atcctcgggc gaattgggca
     2581 ggtcgaaatt caccacatag ggcagttcgg cgatatccag cccgcgggcc gcgacatcgg
     2641 tcgccaccag tacgccgtcc ttgccgctct tgaattcctc cagcgcctgc gtgcgttcgc
     2701 tctgcgtctt gtcgccgtga atcgcctgcg cctgcacgcc ttcgcgggcg agttgataag
     2761 ccagccggtt ggtgcccaga cgggtcgaac agaacaccag cacctgcttc atgtcacgcg
     2821 acttgatgag atgcaccagc aactcgcgct ttttctcgcg cgccacggga tgcaccagat
     2881 gcgagaccga ctcggccgtc tggttgcgcc gcgccacttc caccagcgtc ggctgcttga
     2941 ggaaggaatc ggcgagcttc ttgatctcgt cggaaaaggt cgcggagaac agcagaccct
     3001 gtttgcggtt ggtcagcagc gcgaggatgc gacgcacgtc cggcacgaag cccatgtcga
     3061 gcatgcgatc ggcctcatcc agcacgacga tctcgacctg cgacaggttg agcgtgcgct
     3121 gctgcacgtg atcgagcaga cggcccggcg tggcgaccag cacttcgaca cccgcacgca
     3181 gttgcgcgat ctgcgggtcc atcggcacac cgccgtagat gcaggcgctg cgcaggggca
     3241 ggtacttggc gtagctcctg atgctctctt cgacctgggc cgccagttcg cgcgtcggcg
     3301 cgaggatgag ggcacgcacg gggtggcgcg ccggcgacgc actggtgttg gcatggggcg
     3361 ccagacgctg cagcaacggc agcgcgaagc tcgcggtctt gccggtaccc gtttgcgcgc
     3421 cgcccatgac gtcgcgcccg gccagcacca gcgggatcgc ctgtgcctgg atgggcgtcg
     3481 gttcgttata accctgttcg gcgactgccc tgagcagttc gggaatgagg ccgagttcgg
     3541 tgaacgtggt caaaaagcgc aactccagat tgcgcccggg cgctgccgta cttgctgaaa
     3601 ccgaggatgg tgctgaactg ctgaggaacg gcaggcgccg agcagaaggc cggcattata
     3661 taacagaccg ggccgcgcgc ccggtccgcc tctataatgc cgccctgcac gctttcattc
     3721 cgacgccctc cactcatcga tgatcaccct ctcgcggctg tcgctgcgtc gcggcgccaa
     3781 gctgctgctc gaccaggccg acgccgtcat ccaccccggc caacgcgtcg gcatcgtcgg
     3841 cccgaacggc tgcggtaaat cgagtctgtt cgccctgttg cgcggcgacc tgcatgcgga
     3901 tggcggcgat gtcgccgtgc cgcccggctg ggtgatcgcg catgtggcgc aggagacacc
     3961 cgcggcggaa cgcagcgcca tcgactacac gatggacggc gacggcgaac tgcgcgcggt
     4021 ggaggcggaa atcgccgacg ccgaagcgca ggacgacggc gagcgtctgg cccatgcgca
     4081 cgagcgctat cgcgaaatcg acggctacgc cgcacgcgcc cgcgccgccg cattgctgga
     4141 cggtctgggc ttcgccccgg acgatatcga acgcagcgtg cgcgaattct ccgggggctg
     4201 gcgcatgcgc ctcaacctcg cgcaggcgct gatgtgccgc tccgatctgc tgctgctcga
     4261 cgaaccgacc aaccacctcg atctcgatgc cgtgctgtgg ctggaaggct ggctgcaaag
     4321 ctatcgcggc acgctgctga tgatttcgca cgaccgcgaa tttctcgacg gcaccatctc
     4381 cgcggtgctg catttcgacc ggcagaaatt gaagcgctac agcggcaact actccgcctt
     4441 cgaagaacag cgcgccgcgc agttggcgca acagcaggcc ctgcatgcgc gccagcagag
     4501 cgagatcgcg cgcctggagc gcttcgtcga gcgcttccgg gcgaaggcca ccaaggcacg
     4561 ccaggcgcaa agccgcatga aggcgctcga acgcatggag cgcattgcgc cggcgcatgt
     4621 ggactcgcct ttccacatcc gcttccgcga gtttcccggc ggcccggatc ccatgctgac
     4681 gctggaggac gccagcgccg gctacggcga tacgcctgtc ctgcgcgaca tccgcttcac
     4741 cattcccgcc ggtgcgcgca tcggcttgct cggccgcaac ggcgcaggca agtcgacgct
     4801 gatcaagttg ctcgccggcc gtctcgcgcc gcagtccggc aagcggcgcg aaggcaagga
     4861 tctgcgcatc ggctatttcg cccagcatca actcgaagat ctgcgcgcag acgagacgcc
     4921 gctgcagcac ctcgtgcgct tggacagtgc gacgcgcgaa caggaactgc gcgactacct
     4981 cggcggcttc ggtttcgccg gcgatatggc gctggcgaaa gtcgccggct tctccggcgg
     5041 cgagcgcgca cgactggcgc ttgcgctcat cgtctggcag cgtcccaatc tgctgctgct
     5101 cgacgagccg accaaccacc tcgatctcga tatgcgcgaa gcgctcaccg ttgcgctgca
     5161 ggaatacgaa ggcgcgatgg tgctggtctc gcacgaccgc catctgctgc gcaccgcctg
     5221 cgacacgctg atgcttgtcg ccgacggcgc agtcagcccc ttcgatggcg atctggagga
     5281 ttaccgcgac tggcttgccc aacggcagcc gcgcgacgac ggtcgcgcga agtcgaccgc
     5341 aggcgaccag cggcgcgagc agaaacgcgc cgaagccgaa gcgcgcaacc ggctcgccca
     5401 ggcgcgcaaa cccatcgaga aacgcctgcg cgccgtggag agcgcgatgg ccaaactgaa
     5461 cacagacaag gcgcggttgg aggcgatgct cgctgccgag gacttctata cgcgcagcac
     5521 gccggagcag gttaccgcgg cgttgcgaga gcaggcgcag gtcgcccacg aactggagca
     5581 agcggaaacc gaatggtttg cgctacagga agagttggaa gcgctgagcg cctgagcgcg
     5641 gccgcgtccg ccgcgtttca gttgatggtg gcgtggtcgc cgttggcgta gaccgggacg
     5701 gaggtcgatg ccacgcggag cgtcaggtcc cagtcggatt tggccacacc gttttcgatc
     5761 agcttcaaca tgccgtgcac cagcttctcg tccagattga tgtgcacgct ttcgccgttc
     5821 gccggatgca gattcagcac atgctggcct ttgtcgcctt tgcgcgcatc gacgcgcgcg
     5881 acgagcagcg gttccatccc catcggataa ctgcgcgcca cctcggcgta gggctgattg
     5941 aactccgctt cgctgaccgc gaccgcatgc cgaaagccga gcagcgcacg acgcgcttcc
     6001 gggttgggct gcagaatgat ttccggaatg gcgtgcgcga catcgagcag gacactccaa
     6061 agacgcagga cgacgcgtcg ggtgagccag aacaacactt cggcattctg atcggtcgcc
     6121 agacgcagta acaaccgatc ctgttccacg ttgtattcga gtttaagttg gtgcagacgc
     6181 atgacgggac gctgagacaa actacgacga gtttaaaaca gcgccccgtc agccattgcg
     6241 gcatacgtca cgggggagga cctccccgcg gcgttgtcct ggtcggactc gcacgtcgcg
     6301 gcagcgcgcc cgactaaagc gagcgcgcgc cgccgctcct tacaactgct acttcaacac
     6361 atcgctctgt tgcgcctgct cgacgtaccg cgccacatcc tcgggcagca gcaggcgctg
     6421 cttttccagt ttacgagcga ccttttcgac ggccttcaca tagccagcgt ggttcttgta
     6481 tcgctcctgc agcgacagac gcggatcgcc cgcggccaga cgttccgcct tcgtccgttt
     6541 gaacggaatc gtctgacccg agctctcgca tccgtcgtcg ccgccgaact cgggcgcgcg
     6601 caacgcccaa cccgtggtcg tggcaatcgg cgccgcgaca ggcggcaacc ggatgccggc
     6661 gacctcattg ccgtccttat ccactttcga cacgaagtgc gcataggtcg gacgcccgac
     6721 gacgctgggc ggatagttgg tcaggatgcc gtcatcgaac atcgggccga agtccaggcg
     6781 atagcgcgtg ctgatgacac ctgtgtattt cacgcccgga atgttcggcc acccaagttc
     6841 gctctgcggg acgaagccca cttgcgatcc cggaaccggc accgcgaaag ccgccgtctt
     6901 tttcttggac gcgctcggta cgtcgctctt cggcgggcgc ttgttctgcg tcacccattc
     6961 gtcgagcgcg atgaacaaag cgcgcaatgc gggattcggg ttggtggtgt tacgcggttg
     7021 ctggcagatt ccgggggagt tgggcgggct gccgttgacc gtgtgctcga caccggacag
     7081 caggaagaac cgcacgttat ccggatcctt cagatcattg ccctggagat cggtatgcag
     7141 caacgagcct gatttcaccc aatactcgtt cgacgaattc acctcgaaca ccttcggaca
     7201 cgtgtccgtt gcggcgcatc gttcgatgcg tccggccgtc ttgccggaaa gcgggtcgga
     7261 caggaccggc cacgcgaacg ggaagagatt ctccggatag aggtggttct ggcgattgcg
     7321 ttcggtacgc gccgtctggc tgaagcggaa attgatcgcg tcgccactgc cgcctgcgat
     7381 ccagttctcc acaccgtcga acacccgtcc gccctcttcc gcctcgttga agcccagcgt
     7441 ctgaaagtcg ttcatgtaac gaccgggctg cgaaatggtg aaagcaaggg tgtactggat
     7501 gtcgcccgca agcggattgg gattgccgaa atcatcgacg ttggcgttgc gtagaaagga
     7561 tacgaagtcg cgcgtcgccg ccagtccgat cccggtcacc aacggatcct tcgccgtgta
     7621 ggtgaactcg tagatatgac tttgctggaa cggcgtgccc tcgggcagca gacgaatggt
     7681 gcgctcgtcc acgtattccc agccgtcggc cggtacggta accggcgtat cgttcaggtg
     7741 ctcgcgcacc gtcagcgtcg cctctccctt gtcgagcgtc gcagcggcat aggaaagtcc
     7801 gtacgtgagg gacgtgggat tgtcgaacac gatgtattcg tacgacggtc ccgtgatggt
     7861 cgacccgtcc gggttcgtgg cgaccgggac gttgatggtc agtcggtcac cgccgggcgg
     7921 cgcggaagga tcccatccgt tccagacgag cgtgtagccg cggttcatga gaaaagcttc
     7981 gcccgcatgt tcggccgtcg tcgggttgtt gccgccgccg ctcaggttga agccgccgaa
     8041 cagcttgccg ccgcggttgt tcacctccat gaacatcttg tgattgccct gcgcgagatt
     8101 gatgggcttc aaaatataga agtccatcga gtactcgacg cgatcgttcg cattgagcgg
     8161 cgccagttcg atgtcggtga tcaccgcatt gcgcggatcc ccggggtcca gttcaccgta
     8221 agcgataccg cgcagctttt cgtaggcgcc aacgctgccg aatgtccggc cctcgaacgt
     8281 cggcgactcc acgctggtaa cgtcgatacg ggtgatgcgc gcttctgcag gggcagcgat
     8341 caaagctgtc ccgagtatcg ccacggcaat cgccgtggtg gattggggaa cgcgctttct
     8401 tcgatgccac agcaatgcac cgattccgcc gagcagcaac aacgccgtac ccggctccgg
     8461 cacgcccgtc ggctcgactg ccagcgaaag gctgtaggca tacgggcctt cgtcgtcgct
     8521 gagaaacccg ccgagcacga ccgtgtagtc gccgctgaag ggcaatacga aaccctccaa
     8581 cagcggatcg ccccccggac catcattgtc gatttcgtcg tcggcgaagg taaggaactg
     8641 caatcctccc cagtcggcgt ccgcaaggaa ttccgaatcg tccgcatccg taatgccgaa
     8701 gtacagcgag aacgcggggt cgagcgcagg gtcaatgcgc aaaccctcga gggtgatgac
     8761 gtcacctacc gatccggaaa atcgccagta atcgacgccg gcaccatctt cgtcgcccca
     8821 actgaacccc ccgacggagc cgcccacagt cgtgcccgga gcgatcgtcc cttcataaga
     8881 aaccggcgcc gcctcgactg ccggagtggc cagtgcgaga aacagcaccg tcgtcgccaa
     8941 tgcttttgca aacatgtcgg cctcctcacg atgtgcagga cgaatgcctg tacggaggct
     9001 tggaggcaca agctatgcct tttgatctat gtgctttgaa atcaatatat gtcgattcgc
     9061 tatcgcatac cgatgtaaag ccgtgcgacg cattcggaga atcaggcggt gtatcgcagt
     9121 tgcatcacgc cggaggtatg gcgcttagcg tcgaccaatg tcagctcctc gcgcaacgat
     9181 gcgccatcga gcagcggcac cccttcgccc aacaggatgg gaaggtacga aatgacgtat
     9241 tcggtaatca gcccttgcgc ggcgaaactc gcagcgagct tgccgccgcc gaccagccag
     9301 gcgcgcttga gccagcgaga ttcgagcgcg gccagcgcct gggccggcga ctggttcaat
     9361 accgtgacac ccggcgggac gcgctcgaat gtgcggctgg aaaacaccca gcacggcttg
     9421 tcggcgtacg gccagcgctc gtagcgcaga cattgttcga aagtgcaact gcccatgagc
     9481 acggcatcga tgccggcgta gaacgccgcg tagccgtaat cctcatgcgc cgtttcgaaa
     9541 ggcttgagcc aggcagtgcc gccgtccgct gcggcgatgt agccgtccag gctcgcggcg
     9601 atgtagtacg agacgtccat cgacgcgcct aagcctcgtg acgcgcgagc gcccaggcga
     9661 cgtgctcgcg cacgagttcc gaagaatggt cggcgcgtgc gcgcaaggcg gtgagaaccg
     9721 cattgctcgt gggcgcattg cccagaccca cggcgatgtt gcgcagccag cgttcgtaac
     9781 cgatacgaag gatggcgctg ccggccattc gctgccgaaa ctcgtcttcg ctccagccga
     9841 acagttcgac cagcgtcgcg cggtcgaggc cgtgacgcac ggcgaaatcc gcttcgcgcg
     9901 ttcgctgcgc aaacttgttc cagggacaat gcagctgaca gtcgtcgcag ccgtagacgc
     9961 ggttgccgat gagcgggcgc agttcgagcg gaatgctccc cttcaactcg atggtgagat
    10021 aggagatgca gcgccgcgca tcgaccacat aaggcgcgac gatcgcctgc gtgggacaga
    10081 cttcgataca gcgcgtgcag gtgccgcaat gcgcggagat cggcacatcg accggcagcg
    10141 gcagatcggt gaagagctcg cccaggaaaa aaaaggaacc cgcctcgcgc gtgagcagca
    10201 ggctgtgctt gccgcgccag cccaggccgc tgcgacttgc caattccact tccatcaccg
    10261 gcgcggaatc gctgaagacg cgatagccga attcgccgac ttcggcggcg atgcggtcgg
    10321 cgagttgctg caggcgtgcg cgcagcacct tgtgatagtc gcgacccagc gcatagcgcg
    10381 agacatacgc gcggtcggca tcggccagcg cggcatcgct gtccgccgcg tcgggccagt
    10441 aacccatgcg cgcactgatc acgcggatcg tgcccggcac caactcgccc ggccgcgcgc
    10501 gcttgagacc atggcgtgcc atataatcca tttcgccgtg catgccctgc tccagccagt
    10561 gcagcaaacg cggctcggca tcgcccagat tcacatcggc aatggtcacg gcatcgaaac
    10621 ccagttcgcg cccccatgcc ttgacggcga gtgcgagcgc cggccagtcc cgttcgacga
    10681 gttgctcggt agtgctcatg ccgggttcgc tttacgccct cgaagtccgc taaggttccg
    10741 caatggtagc gcccctcgcc ctccatctct ccgacgaagc cgcgaccgat gcactcggcg
    10801 cgcgactcgc gcgcgcactg acgccgggcc tgtcgattca tctggtcggc gaccttggcg
    10861 cgggcaagac cgccctggtt cgggcctgtt taagggctct gggacatacg ggaaaggtga
    10921 aaagtcccac atataccctg gttgaacctt atgcggtttc taagttaccc ttgtaccact
    10981 ttgatttcta taggctccgc gagcccacgg aatggctgga cgccggcttt cgcgattact
    11041 tcaacgccga ctcggtgtgt ttggtggaat ggccggaaaa agcggccgaa ctgcccccgc
    11101 cggatctgcg gatttcgctc cgtcacgcgc cctcgggccg cgacgcgacg ctgaccgcct
    11161 acagcccaca gggcgagcgg ctgttgcagt ccatttcgag cgccgccggc tgagtctgca
    11221 ggcgctcgcc gccggcgcca tcgtcgccgt cgttccattc gaagttgcac acgccacatc
    11281 gggcatccgc tcggtgcgca tgtggccggc gcaggactac acccgcctca ccatcgaatc
    11341 cacgcagtcg ctcaagcatc gcttgatcac gctcagcaat ccggatcggg tcgtgatcga
    11401 tatcgaaggt gccgacctgc aagcgctgca gcgtgaagcc gccaacaagg tgctggcgca
    11461 cgacccctac gtcatgcaga tgcgcgtcgg gcagttcacg ccggaggtcg tgcggctggt
    11521 catcgatttg aaggaaggcg tcaaaccgca gttgttcacg ctcgccccca tcggcgacta
    11581 cggtcaccgc ctggtgctcg atctttatcc catcaatccg cccgatccgc tcatggcgct
    11641 gctgagggaa caggccgagc gcgacgcggt gcgcggcccc gcgcctctgc cgcagcgcac
    11701 catcaccatc gtggtggatg ccggtcacgg cggcgaggac ccgggcgcga tcggtgcgcg
    11761 cggcagcaag gaaaaggacg tcacactgaa ggtggcgcgc aagctgaagc agttgatcga
    11821 cgccgagccg aacatgcgcg cggtactgac gcgcgacggc gactatttcg tgccgctgca
    11881 catgcgcgtc gccaaggcgc ggcaggtgcg cgccgacctg ttcgtctccg tccatgccga
    11941 tgccttcatc aaaccgcatg cgcgcggctc gtcggtgttc gcgctgtccg agcgcggcgc
    12001 caccagtgcg gcggcgcgct ggctggccaa tcgcgaaaac gagtccgacc tgatcggcgg
    12061 cgtgaacctg gacgtgaagg accgctatct tgcgcagacg ctgctcgatc tgtcgctgac
    12121 ggcgaccatc agcgacagcc tcaaactcgg caagctggtg ctgaccgagt tgggcgggat
    12181 caacaagctg cacaaggaat atgtggagca ggcgggcttc gccgtgctga aagcaccgga
    12241 cattccctcg atcctcatcg agaccgcgtt catcagcaat ccggaggaag agcgccggct
    12301 gaacgacaat gcctatcagg acaagatggc gcgcgccatc gtgacgggca tcaggaacta
    12361 cgtgcaaaag tacccgctgg gcggaccgtc cacgctggct gcacggacga agcaaccgga
    12421 cgcttaagac tccgcgcgca ggcgcgcatt gcggctgcgc gcgcgcagat attccaccgc
    12481 catgggcatc agcgacaggc cgatgatggc gaaaatcacc agcgtcagat tgttcttgat
    12541 gaacgggata ttgccgaaga agtagccggc gtagaccagc agcacgaccc acagcagcgc
    12601 gccgatgacg ttgtaggcga tgaatcgccg gtaggccatg cgcccgatgc cggccacgaa
    12661 cggcgcgaag gtccgcacca aaggcacgaa ccgggcgatg atgatcgtct tcccgccgtg
    12721 cttctcatag aaggcgtagg tgcgatcgag cgccttcttg ttgaaaatcc ttgcatcgtc
    12781 ccactggaag acgcgcggcc cgcaccactt gccgatcgaa taattcaccg tatcgcccaa
    12841 taccgcggcg atgaccagca gcgcggcgag cagcatcacg tccatcccgg cggccgccca
    12901 gatggtgccc gccacgaaca gcagcgaatc gccaggcaga aagggcgtca ccaccagccc
    12961 ggtctcgcag aaaatgatcg cgaaaagaat ggcgtagatc cagtgcccgt attgctgcac
    13021 cagggaagcc agatggatgt cgaggcggac cagcaagtcc cagaacgcga ggaggatgtc
    13081 cattgggtca gcattctacc ggcccgccta taatccgggc gttcgaatcc accagccgac
    13141 gcaatttcac aaggcggcca cagcccttca ttccatgtcc aagattcgcc ccctgcccga
    13201 cacgctcatc agccagatcg ccgccggcga ggtggtggaa cgtcccgctt cggtgttgaa
    13261 ggaactgatg gagaacagcc tcgatgccgg ggcgacttcg atcgccgtga gcctgagcga
    13321 aggcggcggc aagcacatcc gcgtcaccga cgacggtggc ggcatcgacg ccgaagacct
    13381 gtcctacgcg cttgcgcgcc atgcgaccag caagatcgcc accctgaacg atctggagcg
    13441 cgtggcgacg ctgggctttc gcggcgaggc gctggcgagc atcggctcgg tggcgcgcgt
    13501 gtcggtgacg tcgcggacgg ccgacgcgcc gcacgctttc cgcatccgcg ccgaaggcgg
    13561 cccgccgggc gcggtcgaac ccgcggcgca tgcggtcggc accacggtcg aagtgaacga
    13621 tctttacttc aacacgccgg cgcggcgcaa attcatgcgc aaggaagcga ccgagttcgc
    13681 gcattgcaac gatgtgttcc agcgcatggc gctggcgcga cccgatgtcg ccttcagcct
    13741 gaagcacaac gggcgtgtga gcgcgcatct gcccgccgcc gaccttgccg cacgcgccgc
    13801 ggccgtgctg ggcgcggaca tgcaggaggc cgcgcgcctg gtcgacgcgg aagtgccggg
    13861 gatcgcattg cgcggctttg ccggctcgcc ggcgcacgcg cgcggcaacc gcgacgcgca
    13921 atacatctac gtgaacggcc gcttcgtgcg cgacaaggta ctcgtccatg cgttgcgcga
    13981 agcctatgcc gacgtgctgc acggcgaccg tcatgcctcg tatctgctgt ttctggaaat
    14041 cgatccgggt gcggtggacg tcaacgtgca ccctgccaag accgaagtgc gtttccgcga
    14101 cagtcgcgcg gtgcatcaat tcgtctacca cgcggtgtcg cgtgcgctgg ccggttcggt
    14161 cgcggccgca cctcgacccg tcgccgatcc gctggagcat gtctcgccgc accctgaagc
    14221 gccgccgctc tatcgccggc aggacggact cgccgtcgcg caaccggtgg ccgcttatac
    14281 gtcgatgttc gcggacgcct cagcagccga tgcgcccgcc ccggcgccgc ggccggcacc
    14341 ggcgcagtcg gcggaacacg acagcgccgc tcccccgctc ggttatgcac tggcgcaatt
    14401 gcacggcatc tacatcctgg cgcagaacgc caccggactg gtgctggtgg acatgcacgc
    14461 cgcgcacgaa cgcatcctgt acgaaaaact caaggcggcg ctcgaccagc acaagatcac
    14521 cggccagcaa ttgctcatcc ccgtcacctt ccacgccgaa gcgctcgatg tggccacggc
    14581 cgaggaggaa cgcgaaaccc tggctcaact gggtttcgac atcgcggccg tttcgccgac
    14641 cgcgctcgcc gtgcgcgccg tgcccgacat gctggcaaac ggcgacgttc ccgcgctcgc
    14701 ccgcgcattg ctgcaggata ttcgcgaata cggcgccagc caggtgctga acgaacgcca
    14761 gaacgaactg ctcggcacca tggcctgtca cggcgcggtg cgcgcacgtc gcgccctcag
    14821 cgtgcccgag atgaacgcgc tgctgcgcga catggaggcg accgaacgct ccggccagtg
    14881 caatcacgga cggcccacct ggtatcagat gtccctgacg gatctggaca ggctg
//
LOCUS       DATGOU010000129         4908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_6176947, whole genome shotgun sequence.
ACCESSION   DATGOU010000129 DATGOU010000000
VERSION     DATGOU010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6176947"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="VKZ48_07650"
     CDS             complement(<1..522)
                     /locus_tag="VKZ48_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HLU77760.1"
                     /translation="MRGNPSNPARRRSFWLKQLHQWHWISSAICLIGMLLFALTGITL
                     NHAAGIEAQPRVTSLEASLPPALLDEVRALAPADGAAAPAALRQWLAEQLRIDVGDAA
                     AEWSADEIYFSLPRPGGDAWLSIDRATGEAQYERTDRGWISYLNDLHKGRNTGTAWRW
                     FLDIFAVATLVFCL"
     gene            675..2555
                     /locus_tag="VKZ48_07655"
     CDS             675..2555
                     /locus_tag="VKZ48_07655"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c/FTR1 family iron permease"
                     /protein_id="HLU77761.1"
                     /translation="MFGAAAAQPQAEVIVHMLDYVAVDYATAVQDGKVANEAEFREMQ
                     DFTMQIEQQVQQLPAAPMHPMLHEDARTLAARVNEQAAPDEIARRASDLRWDLIAAYD
                     IAVGPRDVPDLAAGAKLYTSLCASCHGATGRGDGPLATTDMDPAPSDFHDDERMRARS
                     VYGLYSTITMGVAGTAMAPFAEQLSEEERWSLAFHVASMHTDTAAIAQGKAMWESGAG
                     RGLFASLRDIAAHSINEAVESGGPDMAPLLAYLVSNPEAADAAADMAIETTRRLMTQS
                     LDAYAKGDHEAAQRLAVSAYLEGFELVEAGLDTLDAQLRLAIEEEMIALRGLLRVRAP
                     VEEVRAHVQRIETLLDRADALLSADGLSPAAAALSAFIILMREGLEAILVLAAVFAFL
                     VKAERRDALRWVHAGWIGALALGLFTWFAASYLIEISGAGRELTEAITALIAAAVLLY
                     VGFWLHGKAYARAWQRYVREKLSGALTRGTLWALAVVSFLAVYREVFETVLFYQALWT
                     QTGEGGAMAIVGGFGAAVFALALISWLVFRYGVRLPIGPFFGASSMLMALLAVVFVGK
                     GVAALQEAGYLAIDAVSFPRIPALGIYPTEQSLGAQLVVIAAVLIGFGWSYGAARKQA
                     RA"
     gene            complement(2610..3158)
                     /locus_tag="VKZ48_07660"
     CDS             complement(2610..3158)
                     /locus_tag="VKZ48_07660"
                     /inference="COORDINATES: protein motif:HMM:NF042855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6806 family protein"
                     /protein_id="HLU77762.1"
                     /translation="MRTEVHVHGTVPLRHGVNLAELEAALRPWLEYIDEDSLADARSA
                     YEDEPGVSFDPQQRVLEICWTGDVGRNFRETIEVALQGLNPYTERAAEVEVSYYYEDG
                     QDEFGIVFVGPTAEAIHEAQRQAMIEDVSHLLSRQFGEAEIGEVVALLNQLFSRATPT
                     ASSGENASPHFIMAPSNRRRLH"
     gene            complement(3227..3412)
                     /locus_tag="VKZ48_07665"
     CDS             complement(3227..3412)
                     /locus_tag="VKZ48_07665"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HLU77763.1"
                     /translation="MPLIRVEMFPGRTQEQKREFAQAVTREASRILQCDPEAVDIIFA
                     ELRKEDWATGGTFWSER"
     gene            complement(3536..>4908)
                     /locus_tag="VKZ48_07670"
     CDS             complement(3536..>4908)
                     /locus_tag="VKZ48_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020701568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HLU77764.1"
                     /translation="IISSEQMLDAYASTGMPIGYHHWSYGKEYLRHEQAYRKGMRGLA
                     YELVINSNPCIAYLMEDNTMTLQALVIAHACYGHNSFFKGNYLFRQWTSPDAIVDYLV
                     FAKNFIMQCEERHGVEAVESLLDACHALQDYGVDRYRHPRPLSADEEAARLREREAHA
                     WRQYDDIWRTVPTKPQKDNDKNHDIFPPEPQENLLYFVEKYSPKLESWEREVIRIVRK
                     LAQYFYPQGQTKLMNEGWATFWHYTLTNRLFDKGLVDDGFMVEFLHLHTNVVCQRGFD
                     ERGYGGINPYALGFAMMQDIKRICEQPDEEDRHWFPDIAGGDWRKVLDFAMRNYKDES
                     FITQFLSPRLIREFRLFAIGDHEDNPYYEIDSIHNDQGYRRVRHLLAAQYNRDQLLPD
                     VQVVRYDRDGDRSLTVRHRQHRNRPLNEDSAKVMRHLAQLWRFPVRLETVNHAGRRID
                     VEEFWT"
BASE COUNT          787 a         1500 c         1671 g          950 t
ORIGIN      
        1 aaggcagaac acgagcgtgg ccactgcgaa aatgtcgagg aaccagcgcc acgccgtgcc
       61 ggtattgcgg cccttgtgca gatcgttgag ataggaaatc cagccgcggt cggtgcgctc
      121 gtactgcgcc tcgccggtgg cccgatcgat gctgagccag gcgtcgccgc cggggcgcgg
      181 cagggagaaa tagatctcgt cggcggacca ctcggccgcc gcatcgccca catcgatgcg
      241 cagttgttcg gcgagccatt ggcgcaaggc ggcgggcgcc gccgccccgt ccgccggggc
      301 gagcgcgcgc acttcgtcca gcagcgccgg cggcaggctg gcttccagac tggttacccg
      361 aggctgggct tcaatgccgg cggcgtggtt gagcgtgata ccggtcaggg cgaacagcag
      421 catgccgatc aggcagattg ccgaactgat ccagtgccat tggtgtaact gcttgagcca
      481 gaaggatcgc cgccgggcgg ggttggaagg gttgccgcgc atgatgccgg gagattttga
      541 tctttcttat tggataagta aatgcgaaac attatcattt ccgttacctt ctattcaaga
      601 cagaaaaatc gatgactccc ttttcgcctg gtcgccggct ccggcaatgg cttcttgccg
      661 gcctgtttac ctgcgtgttc ggcgcggccg cggcacagcc gcaggccgag gtgatcgttc
      721 atatgctcga ttacgtcgcc gtggattacg cgacggcggt gcaggacggc aaggtcgcca
      781 acgaggccga gttcagggag atgcaagact tcacgatgca gatcgagcag caagtgcagc
      841 aactgcccgc cgcgccgatg cacccgatgc tccatgaaga cgcgcgaacg ctcgccgccc
      901 gcgtgaacga gcaggccgcg ccggacgaga tcgcacgacg tgcatcggat ttgcggtggg
      961 atttgatcgc ggcgtacgac atcgcagtcg ggccgcggga cgtgccggat ctcgccgcgg
     1021 gcgccaagct gtatacaagt ttgtgcgcga gttgccacgg cgcgaccggg cgcggcgatg
     1081 gtccactggc gacgacggac atggatccgg cgccttccga tttccatgac gacgagcgga
     1141 tgcgtgcgcg cagcgtgtac gggctctaca gcaccatcac catgggggtg gcgggcaccg
     1201 ccatggcgcc tttcgccgaa caactgagcg aggaggagcg ctggtcgctc gctttccatg
     1261 tcgcatcgat gcatacggac acggcggcca ttgcgcaggg caaggccatg tgggagagcg
     1321 gcgcaggcag aggtttgttc gcaagcctgc gcgacatcgc cgcgcatagc atcaacgaag
     1381 cggtggaaag cggcggaccc gacatggcgc ccttgcttgc ctatctcgtg agcaacccgg
     1441 aggcggccga tgctgcggcg gatatggcaa tcgagacgac gcgtcgcttg atgacccaaa
     1501 gcctggatgc ctatgccaaa ggcgatcacg aggccgccca gcggctggcg gtgagcgcct
     1561 atctggaagg cttcgaactg gtcgaggcgg gtctcgatac gctggacgcg caactccgcc
     1621 tcgcaatcga ggaggagatg atcgcgttgc ggggtctgct gcgggtccgt gcgccggtcg
     1681 aggaggtgcg ggcgcatgtg cagcgcatcg aaacactgct cgatcgtgca gatgcgctgt
     1741 tgagcgccga tggtctttcg cctgccgccg cggcgctgtc cgctttcatt atcctgatgc
     1801 gcgagggcct ggaagccatt ctcgttctgg cggccgtgtt cgctttcctg gtgaaggcag
     1861 agcgtcgcga cgccctgcgc tgggtgcatg ccggttggat cggcgcgctg gcgctggggc
     1921 tgttcacctg gttcgccgcc tcctacctga tcgaaatcag cggcgccggg cgcgaactga
     1981 ccgaagcgat caccgcgctc atcgccgcag cggtcctgct ctatgtcggc ttctggctgc
     2041 acggcaaagc ctacgcgcgg gcgtggcagc gctacgtgcg cgagaaactc tcgggcgcgt
     2101 tgacgcgcgg tacgctttgg gcccttgcgg tcgtgtcctt cctcgccgtc tatcgcgagg
     2161 tgttcgaaac ggtgttgttc tatcaggcgc tctggacgca gacgggcgag ggcggggcga
     2221 tggcgatcgt cggcggcttc ggcgccgccg tgttcgcgct ggcgctcatt tcctggctgg
     2281 tgtttcgtta cggcgtgcgg ctgccgatcg ggcccttttt cggcgccagt tccatgctca
     2341 tggcgttact ggcggtggtg ttcgtcggca agggcgtcgc ggcgctgcag gaggcgggct
     2401 atctcgccat cgatgcggtg agcttcccgc gcataccggc gttgggcatc tatccgacgg
     2461 agcagtcgct cggcgcgcaa ctggtcgtaa tcgcggcggt gctgatcggc ttcggttgga
     2521 gctacggggc ggcgcgcaag caggcgcggg cttgatgggc ggggcacaga ggcacggaat
     2581 ccgctccgtg tccctgtgtt gctgcagctt cagtgcagcc gtctgcggtt cgacggcgcc
     2641 atgatgaaat gcggcgatgc gttttcgccc gaggaggcgg tcggcgtcgc gcggctgaac
     2701 aactggttga gcagcgcaac gacttcgccg atctccgctt cgccgaactg gcgcgacagc
     2761 aggtgcgaga cgtcttcgat catcgcctgc cgctgggctt cgtggatcgc ttccgcggtg
     2821 gggccgacaa agacgatgcc gaattcgtcc tgcccgtctt cgtagtagta gctgacttcc
     2881 acttccgcgg cgcgctcggt gtaggggttg aggccctgca gtgcgacttc gatggtctcg
     2941 cggaagttgc gccccacatc gcccgtccag cagatttcca gcacgcgctg ctgcggatcg
     3001 aagcttacac cgggttcgtc ctcataggcg ctgcgggcat cggcaagcga gtcctcgtcg
     3061 atgtactcca gccaggggcg cagggccgcc tccagttcgg cgaggttgac accgtggcgc
     3121 aggggcaccg tgccgtggac atgtacttcc gttcgcattg cttctccatc tgatgagccg
     3181 cgcgaggcgc ggcccggcta ttgtcctcct aacgacaacg cgatgctcag cgttcgctcc
     3241 agaaggtgcc gccggtggcc cagtcttcct tcctgagctc ggcgaaaatg atgtcgaccg
     3301 cttccggatc gcattgcagg atgcggctgg cttcgcgcgt aacggcttgt gcgaactcgc
     3361 gtttctgttc ctgggtacgg ccggggaaca tttcgactcg gatcaggggc atatcaatct
     3421 ccggacttgt tcgacagacg cgcggccggg cgggggtgcc gcggctacgc gaggtgggcg
     3481 gctatcatag ccaagaaccc gcctcgcgcg aaccgctccg gccgatccgg cgcgcctaag
     3541 tccagaactc ctcgacgtcg atccgccggc cggcgtgatt gacggtctcc agccgcaccg
     3601 ggaagcgcca cagttgtgcc agatggcgca tcacctttgc gctgtcctcg ttcagcggcc
     3661 ggttgcggtg ctgacgatgc cgcaccgtga gcgagcggtc gccgtcgcgg tcgtagcgca
     3721 cgacctgcac gtccggcagc aactggtcgc ggttgtattg cgcggccagc agatgccgca
     3781 cccggcgata gccctggtcg ttgtggatgc tgtcgatctc gtagtacggg ttgtcctcgt
     3841 gatcgccgat ggcgaacagg cggaactccc ggatcaggcg cggcgacagg aactgggtga
     3901 tgaaagactc gtccttgtaa ttgcgcatgg cgaagtccag caccttccgc caatcgccgc
     3961 cggcgatgtc ggggaaccag tggcgatcct cctcgtccgg ctgttcgcag atgcgcttga
     4021 tgtcctgcat catcgcgaaa cccagcgcat agggattgat gccgccgtag ccacgctcat
     4081 cgaagccgcg ctggcacacg acgttggtgt gcaggtgcag gaattcgacc atgaagccgt
     4141 cgtccaccag acccttgtcg aacaggcggt tggtgagcgt gtagtgccag aacgttgccc
     4201 agccctcgtt catcagcttg gtctggccct gcgggtagaa gtactgcgcc agcttgcgca
     4261 cgatgcggat gacttcgcgc tcccagcttt ccagcttggg cgaatacttc tcgacgaagt
     4321 agagcagatt ctcctgcggc tccggcggga agatgtcgtg gttcttgtcg ttgtccttct
     4381 gcggcttggt cggcacggtg cgccagatgt cgtcgtactg ccgccaggcg tgcgcctcgc
     4441 gctcgcgcag gcgcgccgcc tcctcatcgg ccgacagggg tcgcggatgc cggtagcggt
     4501 cgacgccgta gtcctgcagc gcatggcagg cgtcgagcag cgattcgacc gcctccacgc
     4561 cgtgccgctc ttcgcactgc atgatgaagt tcttggcgaa taccaggtag tcgacgatgg
     4621 cgtccggact ggtccactgg cggaacagat agttgccctt gaagaaggag ttgtgaccgt
     4681 agcaggcgtg cgcgatgacc agcgcctgca gggtcatcgt gttgtcctcc atcaggtagg
     4741 cgatgcaggg gttcgagttg atgaccaact cgtaagccag cccgcgcatc cccttgcggt
     4801 acgcctgctc gtgacgcagg tattccttgc cgtagctcca gtggtgatac ccgatgggca
     4861 tgccggtgga ggcataggcg tcgagcatct gctcggagct gatgatct
//
LOCUS       DATGOU010000130         5583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_2207268, whole genome shotgun sequence.
ACCESSION   DATGOU010000130 DATGOU010000000
VERSION     DATGOU010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2207268"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VKZ48_07675"
     CDS             <1..445
                     /locus_tag="VKZ48_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006026934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HLU77765.1"
                     /translation="ETLERVYDERVQLSPGAESLIARCKELGIRTLLVSGGFTFFVDR
                     LKERLGFDDARSNELEVRDGKLTGQVLGEVFDAKAKAAALEESCAAIGATSAQAIVIG
                     DGANDLAMMTAAGVSIAYRAKPIVKQQATYSLDHVGLDGALNLFP"
     gene            1036..2661
                     /locus_tag="VKZ48_07680"
     CDS             1036..2661
                     /locus_tag="VKZ48_07680"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HLU77766.1"
                     /translation="MREQQRQDEQRIPQRLPDADRPVTTPVAPPAEGEETVLVRTLRF
                     TGEVELLSEAARERIAASVAGQRLGIAGLQALAAEATAALHREGRMLARAILPPQDVT
                     DGTVVIEIVDGAIEAIDFNRDPQSRVKEQRLRDIVNAHVGPSKDKLEEALLRINDHPG
                     VAARARLTPGQAPDTSRLVVDVTQAPRFGARVFANNYGSSATGREQGGAVMTLTDLTG
                     YGDLTHLSGVVSEGQQYASIASSMPVGASGVVASINYGYLDYRNVDAIGRLLGLEGRA
                     HYVSGGLDYALLRSRAANVRIGAEAQWKALTDDSDLGDLQDKRVRSLTLKVAGDLRDL
                     FYGQSLTDWSLGWTFGDLDLSRVPAALAADQASYRTDGSFRRVGASLAHLQNLPGAFS
                     LYGRVEGQWTDGNLDSSEQFTLGGPYGVRGWPVGEARGDMGVLGTLELRYDTAAPAGW
                     GTVQWAAFVDGGRIRLHEDAKGIAPLNACGCNQYALGSAGLALRWSADRFHVSATYAH
                     RIGSNGGRSALTGENVDGKKRKDQFWVHGVLRF"
     gene            2751..>5583
                     /locus_tag="VKZ48_07685"
     CDS             2751..>5583
                     /locus_tag="VKZ48_07685"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HLU77767.1"
                     /translation="MVGEGVRGNRGKKSPRRTLAASLATTTLLASPAWAELPTGGQIV
                     AGSGSIATSGADMTVHQASRKLIANWQSFSIGAGNSVNFVQPNASAVALNRVTGHNTS
                     QILDNLNANGQVFLVNPNGVVIGNGAQVRTGGFVASTLDISDENFLAGHYRFEGVGGE
                     IENLGHIEGKVVALIAPTVRNEGTIQGDTALAAGTDVLLDFDGDGLLSVEVKASTVQT
                     LVENKGLIQADGGVAILTARGASEAMKGVVNNSGRIEAQTLAGQNGRILLLADMQHGE
                     VIQNGVLDASAPNDGDGGFVETSAAKVRIGETARVTTRAAQGQTGTWLIDPSDYTIAA
                     SGGDITGAQLSANLADNNIEIQSSAGTTAGNGDIHVNDAVSWNENTLTLTAARDININ
                     AVMTAGGTSRLEMNTGTANGGDDAVAGGTVKVGFAPGEANGFAGRVDFPSRSGEGFLT
                     INGNNYYVLGADDLGVEGDTSTTTLQGIGNNLNGRYTLGADIDASATAGWDSGAGFMP
                     IGSSSNHFTGDFDGLGHTITNLSINRPGRENVGLFGRATNAALRNIGLVNAAVTGKTS
                     VGSLAGTIDATGDGSAAVTNAYATGRVDGVDSRIGGLVGYHSSGGPATMTNVYTAVDV
                     TGSRMVGGLVGYNYGYNTTITSAYATGSVTGSDYAAGGLAGYHEEGHITDAYAKGDVT
                     GNRGVGGLVGVSAAASKLRRTYASGSVSGNENPGGLVGLNFGGSINSSFWDTEASGIG
                     VSAGGGGRTTAAMKDPFTFINAGWDFDTVWAKSNSGENGGQMMLQSMHPGSYYDGYVH
                     VEGDLSHEYGKTNPDLSGLDITKAGAVDTIDWNITATVNVGNYAWGDLNAGPDNIYWD
                     GIGNLEVTPAPLTVTVNDDSKTYDGVAHGGGNGVTYSGFVNGEDESVLGGVLAYGGSA
                     QGAIDVGTYVISVSGLTATNYSIDYAGGTL"
BASE COUNT         1091 a         1859 c         1719 g          914 t
ORIGIN      
        1 ggaaacgctg gagcgcgtgt acgacgaacg cgtgcaactc tcgccgggtg cggaaagcct
       61 gatcgcacgc tgcaaggaac tcggcatcag aaccctgctc gtttcaggcg gattcacttt
      121 cttcgtcgac cgactgaagg aacgccttgg cttcgacgat gcgcgctcga atgaactcga
      181 agtgcgcgac ggcaaactga cgggtcaggt gctcggcgaa gtgttcgatg ccaaagccaa
      241 ggccgccgcg ctggaggaaa gctgcgccgc aatcggcgcc acatcggcgc aggccatcgt
      301 gatcggcgac ggcgccaacg acctcgccat gatgacggcc gccggcgtca gcatcgccta
      361 tcgcgccaaa cccatcgtca aacagcaggc cacctactcg ctcgaccatg tcgggctgga
      421 cggtgcgttg aacctgtttc cctgagacgt cgttcggctc gtcttatggg gcgagccgtc
      481 atccgaagcg atcaccaagg cgttcggatc acgggagcgg acgcccgacc gctccccccc
      541 gatcacgtga agcgcgcttt cggcttcgaa ttccgaagtg cgcagcgtga ttttcttcgt
      601 cttcgcagca tcatttcatt tgcgcgcgat ttcgtgatcg ccgcgtcatt gctggcgttg
      661 cgcgcgttgc ggcgaatttc tgaccgattc ccgacgtaaa ttcaccccct tcggtttgtc
      721 gttgcaccac aacggtgcct ataatcgccc cgctcgtgtg atgcgaatca catcacacgc
      781 accatccgca ccatccgcag cagccgcagt acccgcatgc gtacaccacc cgtaccttgc
      841 ggtcgcgccg ttcaggcagc acatcgtcgg cgccaagtaa gaagcaataa gtatcaagaa
      901 taagcggcgc acgcatgcgt cgcccgaggg aggaatgctg tgtacgcgca cattcgcgcc
      961 gtggcctttt gggccggcgg cgcattgggc tgcatttttt ttgcggccgc ctacgctcaa
     1021 gacgccggct cgtaattgcg cgagcaacaa cgtcaggacg aacaacgcat cccgcagcgt
     1081 ctgcccgatg cggatcggcc tgttacgaca cccgtcgcac cgcctgccga aggcgaggaa
     1141 acggtgctgg tacgcacgct gcgcttcacc ggcgaggtcg aactgctctc cgaagcggcg
     1201 cgtgaacgca ttgccgcttc tgtcgccggt caacgtttgg gcatcgccgg cctgcaggca
     1261 ctcgccgccg aagccaccgc tgcgttacac cgcgaaggcc gcatgctcgc ccgcgccatc
     1321 ctgccgcccc aggacgtcac cgacggcacc gtcgtcatcg agatcgtcga cggcgccata
     1381 gaagcgatcg atttcaatcg cgatccgcaa tcgcgcgtga aggaacagcg cctgcgcgac
     1441 atcgtgaacg ctcatgtcgg tccctccaag gacaagctcg aagaagcgct gctgcgcatt
     1501 aacgatcatc cgggcgttgc ggcgcgcgcg cgtctcaccc ccggtcaggc gccggatacc
     1561 agccgtctcg tggtcgatgt gacgcaggcc ccgcgcttcg gcgcacgggt gttcgccaac
     1621 aactacggca gcagcgccac cggccgcgaa caaggcggcg cggtcatgac gctgaccgat
     1681 ctcaccggct acggcgatct cacccacctg tccggtgtcg tctccgaagg ccagcagtac
     1741 gcaagcatcg cctccagcat gccggtcggc gcatcgggcg tggtcgcctc gatcaactac
     1801 ggctatctcg actaccgcaa tgtggatgcc atcggacgcc tgctcgggct cgaaggccgt
     1861 gcgcattacg tcagcggcgg gctcgattac gcgctgctgc gctcgcgcgc ggccaatgtg
     1921 cgcatcggcg ccgaggcgca gtggaaggcg ctgaccgacg acagcgacct cggcgatcta
     1981 caggacaagc gcgtgcgcag ccttaccctc aaagtggccg gcgacctgcg cgacctgttc
     2041 tacggtcaaa gcctcaccga ctggtcgctg ggctggacct tcggcgacct ggatctgtcg
     2101 cgtgtgccgg cggcgctggc ggccgaccag gcgagctacc gcaccgacgg cagcttccgg
     2161 cgtgtggggg ccagccttgc gcatttgcag aacctccccg gcgccttctc gctctacgga
     2221 cgcgtggagg gccagtggac cgacggcaat ctcgattcat cggaacagtt caccctcggc
     2281 ggcccgtacg gcgtgcgcgg ctggccggtg ggcgaggcgc gcggcgacat gggcgtgctc
     2341 ggcacgctgg agttgcgtta cgacacggcg gcccctgccg gctggggcac tgtgcagtgg
     2401 gcggccttcg tcgacggcgg gcgcatccgc ctccacgagg acgccaaggg catcgcgccg
     2461 ctgaacgcct gcggctgcaa tcagtacgcg ctgggcagcg ccgggcttgc gctgcgctgg
     2521 tcggccgatc ggttccatgt gtcggccaca tatgcgcacc gcatcggcag caacggcggg
     2581 cgctcggcgc tcaccggcga gaacgtggac ggcaagaaac gcaaagacca gttctgggtg
     2641 cacggcgtcc tgcgatttta gaaataagta gcggcagata aagggattgg gggtcaccga
     2701 tgaaccgcat ccacaaactc atctggtcga agacgcagaa tcgtctcgtt gtggtcggcg
     2761 aaggcgtgcg cggcaatcgc ggcaagaaaa gcccacgccg cacattggcc gcatcacttg
     2821 ccaccaccac gctgctcgcc tcacccgcct gggcggaact gcccaccggc gggcagatcg
     2881 tcgcgggctc cggctccatc gccaccagcg gtgcggacat gaccgtgcat caggcaagcc
     2941 ggaagctgat cgccaactgg cagagcttct ccatcggtgc gggaaatagc gtcaacttcg
     3001 tgcagcccaa tgcctcggct gtcgccctga accgcgtcac cgggcacaac acctcgcaga
     3061 tcctcgacaa cctcaacgcc aacgggcagg tgttcctcgt caaccccaac ggcgtcgtca
     3121 tcggcaacgg tgcgcaagtg cgcaccggcg gcttcgtcgc ctccaccctc gatatcagcg
     3181 acgagaactt cctcgccggc cactaccgct tcgaaggcgt cggcggtgag atcgagaacc
     3241 tgggccacat cgaaggcaag gtcgttgcgc tgatcgcccc cacggtgcgc aacgaaggca
     3301 cgatccaagg cgacacggcg cttgccgccg gcaccgatgt gctgctcgac ttcgacggcg
     3361 acggcctgct gtcggtggag gtcaaagcct ccaccgttca gacgctggtg gagaacaaag
     3421 gcttgattca agccgacgga ggtgtcgcca tcctcaccgc acggggcgcc tccgaagcga
     3481 tgaaaggcgt ggtgaacaat agcgggcgca tcgaagcgca aacccttgcc ggccaaaacg
     3541 gccgcatcct gctgcttgcc gacatgcagc atggcgaggt cattcaaaac ggcgtgctgg
     3601 atgccagcgc gccgaacgac ggcgacggcg gtttcgtcga gaccagcgcg gccaaggtgc
     3661 gcatcggcga gacggcgcgc gtcaccaccc gcgcggcgca gggccaaacc ggcacctggc
     3721 tcatcgaccc cagcgactac accatcgccg ccagcggcgg cgacatcacc ggcgcccagc
     3781 tctccgccaa tctggcggac aacaacatcg aaatccagag cagtgccggc accacggcag
     3841 gcaacggcga cattcacgtc aacgatgccg tgagctggaa tgagaacacc ctgactctca
     3901 ctgccgcccg tgacatcaat atcaacgcgg taatgacggc cggcggcacc tcacgactgg
     3961 agatgaacac cggcacagcc aacggcggcg acgacgccgt cgccggcggc acagtaaaag
     4021 tcggcttcgc acccggcgag gccaacggct ttgcagggcg ggtggatttc ccctctcgct
     4081 cgggcgaagg tttcctgacc atcaacggca ataactacta cgttctcggt gctgacgatc
     4141 tcggcgtcga aggcgacacc agcacgacca cactgcaagg catcggcaac aatctcaatg
     4201 gccgctacac cctaggcgcg gacatcgacg cttccgctac cgcaggttgg gacagcggtg
     4261 cgggctttat gccgatcggc agctcgagca atcacttcac cggcgatttc gacggcctgg
     4321 gtcacacgat taccaacctc tccatcaacc gtcccggccg cgagaacgtc ggtttgtttg
     4381 gtcgtgccac caacgcggcc ttgcgcaata tcggcctagt aaacgccgct gtaaccggca
     4441 aaaccagcgt cggctcactg gcggggacca tcgatgcgac cggcgacggc agcgccgcgg
     4501 tcaccaacgc ctatgccaca gggagggttg acggtgtcga cagccgtatc ggcgggctgg
     4561 tcgggtacca ctcttcaggt ggccccgcga ccatgaccaa cgtctacaca gcagtcgacg
     4621 taaccggcag ccgcatggtc ggcggactcg tcggatacaa ctatggctac aacactacca
     4681 ttaccagcgc ctacgccaca ggctccgtga ctggcagcga ttacgccgct ggcggactcg
     4741 ccggatatca cgaggaaggc catatcactg acgcgtatgc caagggcgac gtgaccggca
     4801 atcggggtgt cggcggattg gtgggagtca gcgctgccgc aagcaagctt cgcaggacct
     4861 atgcctcggg cagcgtaagc ggcaatgaaa atccgggcgg actggtcgga ctcaatttcg
     4921 gtggctccat taactcctca ttctgggata cggaggcctc gggcatcggc gtaagtgcag
     4981 gtggcggagg gcgtaccacc gctgcgatga aagacccgtt caccttcatc aatgccggct
     5041 gggatttcga cacagtctgg gcaaagtcca actcgggcga gaacggcggc caaatgatgc
     5101 tgcaatcgat gcatcccggc agctactacg acggctatgt gcacgtcgag ggcgatctca
     5161 gccatgaata cggaaaaacc aatccggacc tatccggcct cgatatcacc aaggccggcg
     5221 ctgtcgatac catcgattgg aatatcactg ccaccgtcaa cgtcggcaac tacgcctggg
     5281 gcgacttaaa tgcggggccc gacaatatct actgggatgg catcggcaac ctggaagtca
     5341 ccccggcgcc gctcaccgtc acggtcaacg acgacagcaa gacctacgat ggggtcgccc
     5401 acggcggcgg caacggcgtc acctacagcg gcttcgtcaa cggcgaagat gaatccgtgc
     5461 tcggtggtgt gctcgcctat ggtggcagcg cacaaggcgc catcgatgtg ggcacctacg
     5521 tcatctctgt ctccgggctg actgcgacga actactcgat cgactatgcc ggcggcaccc
     5581 tga
//
LOCUS       DATGOU010000131        10418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_4389525, whole genome shotgun sequence.
ACCESSION   DATGOU010000131 DATGOU010000000
VERSION     DATGOU010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 10418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10418
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4389525"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1442)
                     /locus_tag="VKZ48_07690"
     CDS             complement(<1..1442)
                     /locus_tag="VKZ48_07690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HLU77768.1"
                     /translation="MKSRSKKNINARQQLDPYWEREAARYDEPLPSREYILQVLEEEG
                     VPVDEKRLAKLLSITRKEAEGFARRIAAMERQGQLLRNRKNQLLVAEKLQLVAGRVEA
                     HPDGFGFLIPDEGGDDYFLNPGEMKQVMHGDRVMARPGGMDRRGRREASIVEVLERAQ
                     QRIVGRLMKERGIWFVVPSDKRISHDLLIEPGGRAQAKEGQIVTAELVSQPGRYAQPI
                     ARIVEVLGDFGDPGMEIEIALRKHDLPYEFSKEAQKLAAKLPKTVRKSDIGPERVDLR
                     HLPFVTIDGETAKDYDDAVYCERQGKGHKLYVAIADVSHYVTPKDALDQAALERGNSV
                     YFPRRVIPMLPEKLSNGLCSLNPDEDRLAMVCEVVINARGELGPYKFYPAVIRSQARL
                     TYTRVAKLLYGLEADVEPVPDALVPHLRALDGVFRALQQARARRGAIDFETIETEMIF
                     DDNGKIERIVPVLRNDAHKLIEECMLAANT"
     gene            1481..1567
                     /locus_tag="VKZ48_07695"
     tRNA            1481..1567
                     /locus_tag="VKZ48_07695"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1515..1517,aa:Leu,seq:cag)
     gene            complement(1639..2130)
                     /locus_tag="VKZ48_07700"
     CDS             complement(1639..2130)
                     /locus_tag="VKZ48_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HLU77769.1"
                     /translation="MTDLFVSWSDYHRTIERLAGRVYDADWHFQHIVAIGRSGLHAGD
                     LLSRLFRVPLSVLQARSARAGDGEVAFSELAGLASPRGQRVLVVDDLIDTGETLAGAV
                     RKLQPQCGELRTAVLWVRQGAGFRPDYHVAEIAAGCRMHPPAEPYDLSNIEEVARRRR
                     ADT"
     gene            complement(2137..3429)
                     /locus_tag="VKZ48_07705"
     CDS             complement(2137..3429)
                     /locus_tag="VKZ48_07705"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HLU77770.1"
                     /translation="MAKNVVIVGTQWGDEGKGKIVDWLTDHVAGVVRFQGGHNAGHTL
                     VVGGRKTILRLIPSGILREGVICYIGNGVVLSPTALFQEIGELEALGVDVESRLRVSE
                     ATTLILPYHVAIDQAREVAKGADKIGTTGRGIGPAYEDKVARRGIRLQDLFVPERFAE
                     KLREVLDYHNFVLTHWLKGQALDYNQVYDETMGFAPRLQKMLTDVPRALRDANAAGQN
                     LLFEGAQGALLDVDHGTYPFVTSSNTVAGAACAGAGVGPQYLHYILGITKAYTTRVGA
                     GPFPTELDDEVGEGLRTRGNEFGSVTGRPRRCGWFDAAALKRSAQINGLSGICLTKLD
                     VLDGMSELKLCVGYEIDGERTDMLPAGADDCGRCKPVYETLPGWSESTLGIKRLEDLP
                     ANARAYLERVEAVCGVPIDIISTGPDREETIVRRHPYL"
     gene            complement(3460..4605)
                     /locus_tag="VKZ48_07710"
     CDS             complement(3460..4605)
                     /locus_tag="VKZ48_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HLU77771.1"
                     /translation="MRTWLLPEYVEDILPAQALGIERLRARLLELFRVHGYELVIPPL
                     LEYTDSLLTGTGSDLDLRTFKLVDQLSGRMMGLRADMTPQVARIDAHLLNRRGLTRLC
                     YCGTVLHTRPRSFTATREPMQMGAELYGHAGIEADIEIQQLLRAALSACGLVDARLDL
                     SHVGVFAALAKHAGVDAATEAALLQALVAKERPRLWALTEPLNESSRAAFRLLPDLYG
                     GREVLDRARQALPAYPELHAALDDIAAVMDANGGPLSVDLADLRGYHYHSGIVFAAYH
                     GGLPNAIALGGRYDEVGKVFGRARPATGFSLDLRQWVQLAPEAPGANAILAPCVADAK
                     LRERIDALRAAGEVVIVDLPGHEQTRAELGCDRQLVLKNGNWEIVSI"
     gene            complement(4612..4800)
                     /locus_tag="VKZ48_07715"
     CDS             complement(4612..4800)
                     /locus_tag="VKZ48_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HLU77772.1"
                     /translation="MGETFATAIALVLVIEGLLPFLMPRLWRDTFRRIVEHSDGQIRF
                     FGLTSMLLGVLLLIILGP"
     gene            complement(4801..5685)
                     /gene="hflC"
                     /locus_tag="VKZ48_07720"
     CDS             complement(4801..5685)
                     /gene="hflC"
                     /locus_tag="VKZ48_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HLU77773.1"
                     /translation="MRNAMGFVVGGLLILIILASTAMYTVDERQKAIVLQLGEIRKVV
                     EEPGLGFKLPLVQNVRYFDGRILTLDTPDAERYITSEKKNVLVDSFVKWRIKDVNQYY
                     VSVAGDEVRATTRLSQTVNAALREEFGKRTVPEVVSGERDKIMQIVLEKVQDDAERIG
                     VEVIDVRLKRVELPAEVSEAVYRRMEAERRAVAAELRSQGFSEAEKIRAEADRDREVI
                     IANAYSKAQAVKGEGDARATAIYAKAFGANPEFYSFYRSLEAYRNSFADRSDVLVIDP
                     SSEFFRHFKNPSRSASGK"
     gene            complement(5698..6915)
                     /gene="hflK"
                     /locus_tag="VKZ48_07725"
     CDS             complement(5698..6915)
                     /gene="hflK"
                     /locus_tag="VKZ48_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HLU77774.1"
                     /translation="MSLNDPQWGRKGPRGGGDNNNQGPPDLDELWRNFNQRLSALFGR
                     KSPDSGGPAGPGGGQGPNMAQFGGGFGLLIALIVIVWLASGFYTVDASQRAVVLTFGA
                     YTETTDPGLRWRLPFPIQRHEIVNLSQVRTIEVGYRNNVKTKVLRESLMLTDDENIVD
                     IQFAVQYLLKDPEDYQFNIRQPDETVMQVAEAAMREIVGKSSMDFVLYEGREQIAVTA
                     QALMQDILDRYQTGITISTVTLQNAQPPEQVQAAFDDAVKAKQDLERQKNEGQAYYND
                     VVPKARGAASRLTEEAEGYRQRVVANAEGEAARFSQVLKEYAKAPAITRERMYLEVMQ
                     QIFTSTTKVMVDARENGNLLFLPLDKLMQMSSSEITGMSGTSKPSAMDSAPATETNRS
                     RDSLLSRDRGPRP"
     gene            complement(6905..8092)
                     /gene="hflX"
                     /locus_tag="VKZ48_07730"
     CDS             complement(6905..8092)
                     /gene="hflX"
                     /locus_tag="VKZ48_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012079974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HLU77775.1"
                     /translation="MLDSPPRDESVADASKALLVALDFGAPDYEESKSELCLLVGSAG
                     LEVRGVVEGRRDRPDPSLFAGSGKVAEIGRARTDSGAGVVIFNHQLSPAQQRNLEREL
                     NCRVVDRTSLILDIFAQRAKSHEGKVQVELAQLEHLSTRLVRGWTHLERQKGGIGLRG
                     PGETQLETDRRLLGVRVKKLKGQLARLQRQHDVQRRARRRRQVMSVSLVGYTNAGKST
                     LFNALTRAGSYAADQLFATLDTTTRRLYLPEAGQITISDTVGFIRDLPHTLVAAFSAT
                     LEETIHADLLLHVIDSSSPVRDKQIEEVNKVLTEIGADHIPQILVANKIDLSGLEPGI
                     DRDEYANIARVRLSARTGQGLDDLRRALTEAAVARSLDEASASHHTNSDNPSQTTANT
                     DVT"
     gene            complement(8116..8346)
                     /gene="hfq"
                     /locus_tag="VKZ48_07735"
     CDS             complement(8116..8346)
                     /gene="hfq"
                     /locus_tag="VKZ48_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HLU77776.1"
                     /translation="MSNKGQLLQDPFLNTLRKEHIPVSIYLVNGIKLQGQVESFDQYV
                     VLLKNTVTQMVYKHAISTVVPSRPVNLNTDND"
     gene            complement(8430..9776)
                     /gene="der"
                     /locus_tag="VKZ48_07740"
     CDS             complement(8430..9776)
                     /gene="der"
                     /locus_tag="VKZ48_07740"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HLU77777.1"
                     /translation="MKPTIVLVGRPNVGKSTLFNRLTRTRNALVADVPGLTRDRHYGD
                     GRVGDRPYFVVDTGGLEPVAKDGILYEMARQTEAAVAEADAIIFLVDGRDGLTPQDRR
                     IADSLRRESSRVWLAVNKAEGMDPGMVTAEFHELGMGEPHAVSAAHGDGVRALMEEVL
                     DALPVVEEAPKTEEESGVPRIAVIGRPNVGKSTLINSLLGESRLVAFDQPGTTRDSIE
                     VPFERRGKRYILIDTAGVRRKGRVFEAIEKFSVVKTLQAIEQANVVILVLDARLEISD
                     QDAHLAGYIVEQGRALVLAVNKWDGLDEYQREQIRRQLARKLGFLGFATTHYISALEG
                     KGLGAVLASVDEAYKSAMAKLPTPKLTRVMAEAVERQPPPRGGQSRPKLRYAHQGGSN
                     PPIIVIHGTALEHVPDSYKRYLENCFSQHFGLKGTPLRVEFRTARNPYVRSRGKRK"
     gene            complement(9846..>10418)
                     /locus_tag="VKZ48_07745"
     CDS             complement(9846..>10418)
                     /locus_tag="VKZ48_07745"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HLU77778.1"
                     /translation="GGYVFAGFAGGKLVAVDLKNGGLRWEGTVTLPRGTTELERVADV
                     VGQPWVGDRAACAVAYQGRVSCFNLANGSQVWARDISSSAGLGVGTQNVYVSEDAGAV
                     SAFDGASGGSLWRQDKLGNRQVSAPLPLGPEIVVGDVEGYVHWLARENGAFVARQATD
                     GSPIRATPVPFAQGFIVQTTNGGIYALSAE"
BASE COUNT         1740 a         3434 c         3289 g         1955 t
ORIGIN      
        1 caggtgttcg ccgccagcat gcattcttcg atcagcttgt gcgcgtcgtt gcgcagcacc
       61 ggcacgatgc gctcgatctt cccgttgtcg tcgaagatca tttccgtttc gatggtctcg
      121 aagtcgatcg cgccgcgcct cgcgcgcgcc tgctgcaggg cgcggaacac gccgtccagt
      181 gcgcgcaagt gcggcaccag cgcatcggga accggctcga catccgcttc gagaccgtag
      241 agcagtttgg ctacgcgcgt ataggtgagc cgcgcctgcg agcgaatcac cgcgggatag
      301 aacttgtacg gccccagttc gccccgggca ttgatcacga cttcgcacac catcgccaga
      361 cggtcttcgt cgggattgag cgagcacaga ccgttcgaca gcttttccgg cagcatgggg
      421 atgacgcgcc gcgggaaata caccgagttg ccgcgctcga gcgccgcctg atcgagcgcg
      481 tccttcggcg tgacgtaatg gctgacgtcg gcgatggcga cgtaaagctt gtggcccttg
      541 ccctgccgtt cgcagtacac cgcgtcgtcg taatccttgg ccgtctcgcc gtcgatggtg
      601 acaaaaggca ggtggcgcaa atcgacgcgc tcggggccga tgtccgactt gcgcaccgtc
      661 ttgggcagct tggcggcaag cttctgcgct tccttggaga attcgtacgg cagatcgtgc
      721 ttgcgcagcg caatctcgat ttccatgccg ggatcgccga agtcgccgag cacctcgacg
      781 atgcgcgcaa taggctgcgc gtagcggccc ggctgcgaca cgagttcggc cgtgacgatc
      841 tggccttcct tcgcctgtgc acgcccgccg ggctcgatca acagatcgtg gctgatacgc
      901 ttgtcggagg gaacgacaaa ccagatgccg cgctccttca tcagccggcc gacgatgcgc
      961 tgctgcgcgc gctccagcac ttcgacgatg ctcgcttcgc gccggccgcg ccgatccatg
     1021 ccgcccggcc gcgccatgac gcggtcgccg tgcatgacct gcttcatttc cccggggttg
     1081 aggaagtaat cgtcgccgcc ttcgtcgggg atcagaaaac cgaagccgtc cgggtgcgcc
     1141 tcgacgcgac cggcgaccaa ctggagtttt tccgcgacca gcaactggtt cttgcgattg
     1201 cgcagcaact gaccctggcg ctccatcgcg gcgatgcgcc gcgcaaagcc ttcggcctct
     1261 ttgcgggtga tggaaagcag tttggccagc cgcttttcgt cgaccggcac gccctcctct
     1321 tcgagtacct ggaggatgta ttcgcgactg ggaagcggct cgtcgtaccg ggccgcctcc
     1381 ctttcccaat agggatcgag ttgctgccgc gcgttgatgt tctttttaga tcgcgatttc
     1441 attgaatccc ttatccgatt gcactagaat gcgcccctca gccgagatgg cggaattggt
     1501 agacgcacta ggttcaggtc ctagcgcccg caagggtgtg gaggttcgag tcctcttctc
     1561 ggcaccatac aacacgatag aagcccgccc ctggcgggct tcgtcgtttc cgcgatgccc
     1621 aagcgcttgc gcgcgctttc aagtatccgc acgccttctg cgggccactt cttcgatatt
     1681 gctcagatcg taaggctcgg ccggaggatg catccggcag ccggcggcga tttccgccac
     1741 atggtaatcc ggtcggaagc cggcgccctg ccgcacccat agcactgcgg tgcgcagttc
     1801 gccgcattgc ggctgcagtt tgcgtaccgc gccggccagg gtctccccgg tatcgatcag
     1861 gtcgtccacc accagcacgc gctgtccccg cggcgaggca aggccggcca gttcggagaa
     1921 ggcgacttcg ccgtcccccg cacgcgccga gcgcgcctgc agcacggaga gcggcacgcg
     1981 gaatagccgc gacagcaggt cccccgcgtg caggccgcta cggccgatgg cgacgatgtg
     2041 ctggaaatgc cagtcggcgt cgtacacccg accggcaagc cgttcgatgg tccgatggta
     2101 gtcggaccaa ctgacgaaca gatccgtcat gcgcccttaa agataagggt gacggcggac
     2161 gatggtctcc tcgcggtccg gaccggtgga aatgatgtcg atgggcacgc cgcacaccgc
     2221 ttccacgcgc tccagatagg cgcgcgcgtt ggccggcagg tcttccaggc gtttgatgcc
     2281 cagggtgctt tccgaccagc ccggcagggt ttcgtacacg ggcttgcagc ggccgcaatc
     2341 gtccgcgcct gcgggcagca tgtcggtacg ctcgccgtcg atttcatagc ccacgcacag
     2401 cttcagttcg gacatgccgt cgaggacgtc gagcttggtc aggcagatgc cggacaggcc
     2461 gttgatctgc gccgaacgct tgagcgccgc ggcgtcgaac cagccgcagc ggcgcgggcg
     2521 gccggtgacg gagccgaatt cgttgccgcg cgtgcgcaga ccctcgccca cttcgtcgtc
     2581 cagttcggtc gggaacggac cggcgcccac gcgggtggta taggccttgg tgatgccgag
     2641 aatgtagtgc aggtactgcg gccccacgcc cgcgcccgcg caggcggcac cggcaacggt
     2701 gttgctggag gtcacgaacg gataggtgcc gtgatccacg tccagcagcg cgccctgggc
     2761 gccttcgaac agcaggttct gcccggcggc gttggcgtcg cgcagcgcac gcggcacgtc
     2821 ggtcagcatc ttctgcagac gcggcgcgaa gcccatggtc tcgtcgtaaa cctggttgta
     2881 gtcgagcgcc tgtcccttga gccaatgggt caggacgaag ttgtgatagt ccagcacttc
     2941 tcgcagcttc tccgcgaagc gctcgggcac gaacaggtcc tgcagacgga tgccgcgccg
     3001 cgccactttg tcttcgtagg cgggaccgat accgcgaccg gtggtgccga tcttgtcggc
     3061 gcccttggcg acttcgcgcg cctgatcgat ggcgacgtga tacggaagga tcagcgtggt
     3121 cgcctcgctg acgcgcaggc gcgattccac gtccacaccg agcgcctcca gttcgccgat
     3181 ttcctggaac agcgcggtgg gcgacagcac caccccgttg ccgatgtagc aaatgacgcc
     3241 ctcgcgcaaa atgcccgagg gaatgaggcg cagaatggtc ttgcgcccgc ccacgaccag
     3301 ggtgtggccg gcattgtggc cgccctggaa gcgcaccacg cccgcgacgt gatcggtcaa
     3361 ccagtcgacg atcttgcctt tgccttcatc accccattgg gtgcctacaa tgacgacgtt
     3421 cttagccatg ctttcctcga gcactcaccg cagccgcggt cagatgctta caatctccca
     3481 gttgccgttc ttcaagacga gctgccggtc gcagcccagt tcagcgcgtg tctgctcgtg
     3541 gccgggcaga tcgacgatca ccacctcacc ggccgcacgc aacgcgtcga tgcgttcgcg
     3601 cagctttgca tccgccacgc aaggcgcgag gatggcgttc gcgcccggcg cctccggcgc
     3661 cagttgcacc cattgacgca aatcgagcga aaaacccgtt gccggccgcg cccggccgaa
     3721 caccttgcct acttcatcgt agcggccgcc gagcgcaatg gcattgggca aaccgccatg
     3781 ataagccgcg aatacgatgc cgctgtgata gtggtagccc cgcagatcgg ccagatccac
     3841 actcagcgga ccgccgttgg cgtccatcac cgcagcgatg tcgtccagcg cggcatgcaa
     3901 ttcaggatag gcgggcaacg cctggcgtgc acggtccagt acctcccggc cgccgtacag
     3961 gtccggcagc agccggaagg cggcgcgcga ggactcgttc agcggctcgg tcagcgccca
     4021 cagccgcggc ctttccttgg ccaccagggc ttgcagcaac gccgcttccg tcgccgcatc
     4081 cacgcccgca tgcttggcca gcgcggcaaa tacgccgaca tgcgacaggt cgagccgggc
     4141 atccaccaga ccgcaggcgg acagcgcggc gcgcagcaac tgctggattt cgatatcggc
     4201 ctcgatgccg gcatggccgt acagctcggc gcccatttgc atgggctccc gggtggcggt
     4261 gaagctgcgc ggccgggtat gcaggaccgt gccgcaatag cacaggcgcg tcaggccgcg
     4321 acgattgagc agatgcgcat cgatgcgcgc cacctgcggc gtcatgtcgg cgcgcagccc
     4381 catcatccgg ccggatagct gatcgaccag cttgaaggtg cgcagatcga ggtcgctccc
     4441 cgtgccggtc agcagcgaat cggtgtattc cagcagcggc ggaatgacca gttcgtagcc
     4501 gtgcacgcgg aagagctcga gcagccgcgc gcgcagacgc tcgatgccga gcgcctgcgc
     4561 gggcaagata tcttcgacgt attcgggcag cagccaggtg cgcatcgcgg actaagggcc
     4621 gagaataatc aacagcagca caccgagcag catcgatgtg aggccgaaaa aacgaatctg
     4681 tccgtcgctg tgctcgacga tgcggcggaa cgtgtcgcgc cacagccggg gcatcagaaa
     4741 cggaaggagc ccctcgatca ccaggaccaa ggcgatcgcg gttgcgaaag tttcgcccat
     4801 ttacttgccg gaggcactgc ggctgggatt cttgaagtga cggaagaatt ccgaactcgg
     4861 atcgatcacc agcacgtcgc tgcggtcggc aaagctgttg cgataggctt ccaggctccg
     4921 gtagaaggaa tagaactcgg gattcgcgcc gaaggccttg gcataaatcg ccgttgcgcg
     4981 cgcatcgccc tcacccttca ccgcctgcgc cttgctgtag gcgttggcga taatcacttc
     5041 gcggtcgcgg tcggcttcgg cgcggatctt ctccgcttcc gaaaagccct gcgaacgcag
     5101 ttccgccgcc acggcgcgcc gttcggcttc catccgccgg tagacggctt cgctcacctc
     5161 ggccggcagt tcgacgcgct tgaggcgcac gtcgatcacc tcgacgccga tgcgctccgc
     5221 atcgtcctgc accttctcca gcacgatctg catgatcttg tcgcgctcgc ccgaaaccac
     5281 ttccggtacg gtgcgcttac cgaactcctc gcgcagcgcg gcgttgacgg tctgcgacag
     5341 acgcgtggtg gcgcgcacct cgtcgccggc gaccgagacg taatactggt tcacgtcctt
     5401 gatgcgccac ttgacgaagg aatcgaccag cacgttcttc ttttccgagg tgatgtagcg
     5461 ctcggcgtcc ggcgtatcca gcgtcagaat gcggccgtcg aaatagcgta cgttctgcac
     5521 caacggcaac ttgaagccga gacccggctc ttcgacgact ttacgaatct cgcccaactg
     5581 caggacgatg gccttctggc gctcgtccac cgtgtacatc gcggtggaag cgaggatgat
     5641 caggatcagc aggccgccga cgacgaatcc cattgcgttt ctcatgcgat cctccgttta
     5701 gggccgcggg ccgcggtcgc ggctcaacag cgaatcgcgc gagcgattgg tttcggtcgc
     5761 gggcgccgaa tccatggcgg acggcttgct cgtaccgctc atgccggtga tttcggacga
     5821 cgacatctgc atcagcttgt ccagcggcag gaacagcagg ttgccgttct cgcgcgcatc
     5881 gaccatgacc ttggtggtgc tggtgaagat ttgctgcatg acctccagat acatccgctc
     5941 gcgcgtgatg gccggcgcct tggcgtattc cttcaacacc tggctgaagc gtgcggcttc
     6001 accttcggca ttggcgacca cgcgctggcg ataaccttcg gcctcttccg tcaggcgcga
     6061 agcggcgccg cgcgccttcg gcacgacatc gttgtaatag gcctggcctt cgttcttctg
     6121 acgctccagg tcctgcttcg ccttcaccgc atcgtcgaac gcggcctgca cctgctccgg
     6181 cggctgggcg ttctgcagcg tcacggtgct gatcgtgatg ccggtctgat agcggtcgag
     6241 aatgtcctgc atcagcgcct gcgccgtgac ggcgatctgc tcgcgccctt cgtacaatac
     6301 gaagtccatg ctcgacttgc cgacgatttc gcgcatcgcc gcttcggcga cctgcatgac
     6361 ggtctcgtcg ggctggcgaa tgttgaactg gtagtcctcc gggtccttca gcaggtactg
     6421 cacggcgaac tggatgtcga cgatgttctc gtcgtcggtg agcatgagcg attcgcgcag
     6481 gaccttggtc ttgacgttgt tgcgatagcc cacctcgatg gtgcgcacct ggctgaggtt
     6541 gacgatctcg tgccgctgga tggggaacgg cagacgccag cgcaggccgg ggtccgttgt
     6601 ctcggtatag gcgccgaagg tgagcaccac ggcgcgctgg ctcgcgtcca cggtatagaa
     6661 cccgctggcc agccagacaa tgacgatcag cgcgatcagc aacccgaatc cgccgccgaa
     6721 ctgggccatg ttgggcccct ggccgccgcc gggaccggcc ggtccgccgc tatcgggcga
     6781 cttgcgaccg aacagggcgc tcaggcgctg attgaagttg cgccacagtt cgtccagatc
     6841 gggaggaccc tgattgttgt tgtcgccgcc gccgcgcggg cccttgcggc cccattgggg
     6901 atcgttaagt gacatccgtg ttcgctgttg tctgactcgg gttatcggag tttgtgtgat
     6961 gcgaggctga tgcctcgtcg agcgaccgcg caacagccgc ttcggtgagg gcccggcgca
     7021 aatcgtccag tccctggcct gtccgggcac tgaggcggac gcgcgcgata ttagcatact
     7081 cgtcccggtc aatccccggt tccaggccgg acaggtcgat cttgttggcg acgagaatct
     7141 gcggaatatg gtccgcgccg atctccgtca gcaccttgtt cacctcctcg atctgcttgt
     7201 cgcgcacggg gctggacgag tcgatcacat gcagcaacag gtcggcgtgg atggtctctt
     7261 ccagcgtcgc gctgaaggcc gcaaccagcg tatgcggcag gtcacggatg aagccgaccg
     7321 tatcggaaat ggtgatctgc cccgcctcgg gcagatagag ccggcgcgtc gtcgtatcca
     7381 gtgtggcgaa caactgatcc gccgcataac tgccggcgcg cgtcagggcg ttgaacagcg
     7441 tggactttcc ggcgttggtg tagccgacca gtgaaaccga catcacctgc cggcgacgtc
     7501 gcgcgcggcg ctgtacgtcg tgctgccgct gcagccgggc caactgtccc ttgagtttct
     7561 tgacccggac gcccagcagg cggcggtcgg tttccaactg cgtctcgccc gggccgcgca
     7621 ggccgatacc gcccttctgc cgctccaggt gcgtccagcc gcgcaccagg cgggtggaca
     7681 aatgttccag ttgcgccagt tccacctgca ccttgccttc atggctcttc gcgcgctggg
     7741 cgaagatgtc gaggatgaga ctggtgcggt ccaccacgcg gcagttcagt tcgcgttcga
     7801 ggttgcgctg ctgtgccggc gagagctggt ggttgaagat gaccacgccg gcgccgctgt
     7861 cggtgcgggc gcgcccgatt tccgcgactt tgccggagcc ggcgaacagg ctgggatcgg
     7921 gacggtcgcg gcggccctcg acgacgccgc gcacttccag cccggcgctg ccgaccagca
     7981 gacacagttc ggatttgctc tcttcgtaat cgggtgcgcc gaagtcgagc gcgaccaaca
     8041 gcgccttgga ggcgtctgcc acgctctcat cgcgtggcgg tgaatcgagc atcctgcgtg
     8101 tgtctccctg ccctgttaat cgttgtcggt gttcagattg accgggcgcg aaggcacgac
     8161 cgtcgaaatg gcgtgcttgt aaaccatctg cgtcacggtg tttttcagca gcacgacgta
     8221 ctgatcgaag gattcgacct gaccttgcag cttgatgccg ttgaccagat agatcgaaac
     8281 agggatgtgc tccttacgca gagtattgag gaacgggtct tgtaacagtt gccctttatt
     8341 gctcatgttt gctccggaag cgttgttttt gagtaattac tgtaatggat ttccaggggg
     8401 tgtgccatgc aacggcccta ttcgcgccct tatttacgtt ttccccggct ccgcacatag
     8461 ggatttctcg cagtccgaaa ttcgacgcgt aagggcgtgc ctttaaggcc gaagtgctgg
     8521 ctgaagcagt tttccagata gcgcttgtag ctgtccggca catgttccag cgcggtgccg
     8581 tggatgacga tgatcggcgg attgctcccg ccctggtgcg cgtaacgcag tttcggccgg
     8641 gactgaccgc cgcgcggcgg cggttgacgc tccaccgctt cggccatcac ccgcgtcagc
     8701 ttcggcgtcg gcagcttcgc catggccgac ttgtaggcct catcgaccga agcaagcacc
     8761 gcacccagcc ccttgccttc cagcgcggag atgtagtgcg tcgtggcaaa gccgagaaaa
     8821 cccagtttgc gcgccaactg ccggcggatc tgctcacgct ggtattcgtc cagtccgtcc
     8881 cacttgttca ccgcgaggac gagcgcgcgt ccctgctcga cgatgtagcc ggccagatgc
     8941 gcgtcctgat cggagatctc cagccgcgca tcgagcacca ggatgacgac gttcgcctgt
     9001 tcgatggcct gcagcgtctt cactaccgag aatttctcga tcgcttcgaa cacgcgtccc
     9061 ttgcgccgca cgccagcggt gtcgatcaga atgtagcgct tgccgcgccg ttcgaagggc
     9121 acttcgatgc tgtcgcgcgt ggtgcccggc tgatcgaaag cgaccagccg gctttcgcca
     9181 agcaggctgt tgatcagggt ggacttgccc acgttcgggc ggccgatgac ggcgatgcgc
     9241 ggcacaccgc tctcttcttc ggtcttcggc gcttcctcga ccaccgggag ggcgtcgagc
     9301 acttcctcca tcaacgcgcg cacgccgtcg ccgtgcgcgg cagacaccgc atgcggctcg
     9361 cccatgccga gttcatggaa ttccgccgtg accatgccgg ggtccatgcc ttcggccttg
     9421 ttgacggcca gccagacgcg cgatgactcg cgccgcaggg aatcggcgat gcgccggtcc
     9481 tgcggcgtca gcccgtcgcg tccgtcgacc aggaagatga tggcgtccgc ttcggcgacg
     9541 gccgcttcgg tctgtcgcgc catctcgtac aggatgccgt ccttggccac cggttcgagg
     9601 ccgccggtat cgacgacgaa gtacgggcgg tcgcccacgc ggccgtcgcc atagtgccgg
     9661 tcccgcgtga gaccgggcac atcggccacc agggcgttgc gtgtccgcgt caggcggttg
     9721 aacagggtcg acttgcccac attgggacgg ccgaccagaa cgatcgtagg tttcatgccg
     9781 gggccgtcgg tcgaccgctc aatgcttcaa ccgacggaca aatcctttaa cacacgtgcc
     9841 gcgcactatt cggcggacag cgcgtagatg ccgccgttcg tcgtctgcac gatgaagccc
     9901 tgcgcaaacg gcaccggggt tgcgcggatg ggcgaaccgt cggtggcctg acgcgcgaca
     9961 aaggcgccgt tctcgcgcgc cagccaatgc acatagcctt cgacatcgcc cacgacgatt
    10021 tccggaccga gcggcagcgg cgcgctgacc tggcggttgc cgagtttgtc ctgccgccac
    10081 agactcccgc cgctggcgcc gtcgaatgcg gagaccgcac cggcgtcttc gctcacatag
    10141 acgttctggg tgcccacacc cagcccggcg ctggaagaga tgtcgcgcgc ccacacctgg
    10201 ctgccgttgg cgaggttgaa gcaggatacc ctgccctgat acgccaccgc gcaggcggcg
    10261 cgatcgccca cccagggctg accgaccaca tcggccacgc gttccagttc ggtcgtaccg
    10321 cgcggcagcg taaccgtgcc ttcccagcgc aaaccgccgt tcttcaggtc cacggcgacc
    10381 agcttgccgc cggcgaagcc cgcgaacaca taaccgcc
//
LOCUS       DATGOU010000132         8935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3975634, whole genome shotgun sequence.
ACCESSION   DATGOU010000132 DATGOU010000000
VERSION     DATGOU010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8935
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3975634"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VKZ48_07750"
     CDS             complement(<1..512)
                     /locus_tag="VKZ48_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HLU77779.1"
                     /translation="MSRVLVTYRVRAAAADIEAVAESLALEQSVETPRDVVRDAFVRD
                     EVLGRVESIRSLDAEYHEVTLALSVTTMGGDVAQTLNMLYGNSSLHAHVELIDARFPA
                     DFVRHFARPRFGIEGLRALCNAYDRPLTCAALKPQGLPVEALAELCYRFALGGVDLIK
                     DDHGLADQTYS"
     gene            complement(604..870)
                     /locus_tag="VKZ48_07755"
     CDS             complement(604..870)
                     /locus_tag="VKZ48_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HLU77780.1"
                     /translation="MKLGDFAMKAPRRSGSAVLALSGVDDESAARSVRSALTLIRGVR
                     AVQISLAQRQARVAFDPETVQPWQLHRAVQAVGCDAELQTPSKA"
     gene            complement(867..1211)
                     /locus_tag="VKZ48_07760"
     CDS             complement(867..1211)
                     /locus_tag="VKZ48_07760"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HLU77781.1"
                     /translation="MATPLQITFRDMDPSPALEARIREKMAKLEALDERITSGRVVVE
                     RLSARRRSGNEIGVLIELAVPGPNILIDDIRNEDAYVAVRDAFDAAERMLIGANAPER
                     GPRPVVQPEAET"
     gene            1408..3144
                     /locus_tag="VKZ48_07765"
     CDS             1408..3144
                     /locus_tag="VKZ48_07765"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HLU77782.1"
                     /translation="MNSITQSPPQLSGWRRLLPFLQWWAMVDRHTLRADALAGLTGAI
                     ILVPQGVAFATIAGMPPEYGLYACMVPAIIAALFGSSWHLVTGPSTTGSLIVFASLSA
                     LAIPGSPDYIRLALTLAFLTGAFQLALGLARMGVLVNFVSQTVITAYVAGAAVWIFCS
                     QLGNFFDVATPRGMSLVDLLQSFFMQLGDINPYTAAVGVVTLAAGLLTRRLWPRLPHM
                     IVAIVAGSVCAVALDQVFGQDVTNIRTVGAIRQALPPLSLPDFSADAIEATLLSALVL
                     TVLTLTEAVSIARAVALRTDQVIDNNQTFIGQGLANVVGAFFSAYPSSGSFNRTGLNY
                     EAGAKTPLSAILGAVFLVLILVALAPLARYLPITSMAALLFMVAVGLVNVEQIRKAWH
                     ASRWEALVLVVTFVATLVDLRVSIFIGMALSLAVFVYHASRPTVSTAVPHADPNSAHF
                     VDGDGIVPDCPSLKIVRINGALYFGAANYIQQALMRIDRDDPRHVNVLIVARGIHYID
                     AAGAQVLAQEARRRRKLGGALYFYRLSDTAQETLRRSGYLDDIGEANLYPTQTGVLDS
                     VKAAIAARAPTA"
     gene            complement(3183..3638)
                     /locus_tag="VKZ48_07770"
     CDS             complement(3183..3638)
                     /locus_tag="VKZ48_07770"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HLU77783.1"
                     /translation="MNDDRQAKRESDAARASKRAKQEAPARKTRTIESGTAADSKRMN
                     GEGTAAPRKRAAAKRVKAPAQAKSENGTARRPRKPVAAPSVSARESDVDGRDAVPARE
                     LSPEERHRMIQRLAYFRAEWRGWTPGAEMDDWLHAEREVDDMLRRAGGG"
     gene            3876..4952
                     /locus_tag="VKZ48_07775"
     CDS             3876..4952
                     /locus_tag="VKZ48_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HLU77784.1"
                     /translation="MPTTREERMRLERIIESAMDAIITVDDRQQIVLYNRAAAMMFGV
                     PPDDALGGTLERFIPDRYRRARISPIMHFGAAPPGMAERRTGGRTVYGRRANGEEFPM
                     DASISDIDVGDRRFYTVILRDITDRVRAEQALKQAHTELTTLSRAVGTSLENQRRRIA
                     RELHDELGQMLTAMKIDVSELQGQLPKGAADLRERCEQLRLLIDQTIAATRRIATDLR
                     PLVLDDLGLAAAIDWLAENLRKRTGLHISVRFDEAMTHVGEPYASNLFRIIQESLTNI
                     VRHAGATEAQIEVSHDGVEARLMVCDNGRGIRTDETQRHCLGLLGIRERARILGGSAT
                     IAPRTGRGTQLVVTFPYRPGQEAA"
     gene            4952..5614
                     /locus_tag="VKZ48_07780"
     CDS             4952..5614
                     /locus_tag="VKZ48_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010400418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLU77785.1"
                     /translation="MIRIIIADDHAIVRDGLKRIIETAPGCEVVSEAVDGAEVIDRVR
                     TQPADVLLLDLSMPGASGIDLIRHVKSIAPPLHILVLSMHAEEQYALRSIRAGASGYL
                     TKDCASDSLVRALRKVASGSVYISEGVAEQLARHLNLGAMDEVPHRSLSDREYEVFLA
                     LVSGRSVSDIAHALNLSVKTISTHKSRILEKMQLDSVADLVRYAVAHRLIDLPYPNMP
                     SQ"
     gene            5759..6187
                     /locus_tag="VKZ48_07785"
     CDS             5759..6187
                     /locus_tag="VKZ48_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLU77786.1"
                     /translation="MPQELLPPLAASPDLRVFIAERSAHIRARMVSLLDRCAGVAYVG
                     WAESVGECIAGVRRTNPHGLVLDLQLVDGCALPALRALRSDNPGLRVVVCATWATAQH
                     EAACREAGAERLIDKATEFPELSNIVQAWVRSHGVRPVIH"
     gene            6235..7056
                     /locus_tag="VKZ48_07790"
     CDS             6235..7056
                     /locus_tag="VKZ48_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLU77787.1"
                     /translation="MPVIALREPNPSLTMQRRSHAAGYVILRELNGLLHLDLPECAAL
                     DRLDFATRQLRAGESLVHCGDECRALYAVRFGSLRSAMIDADGAEQGLGFPMPGDIVG
                     TDGLATGRHTAHAVALERSEVVIIPLARIAEIARTLPGFDLVLYRIIGRETLRDHATL
                     YLLGSLNAEGRVAAFLLNLSERYGELGYARNVFNLRMTRNDIASYLGLTLETVSRAFS
                     AYASCDLLRVDGREIEILDREALQRAITAPSAVLRGGRAARPQSGRYGGALALAS"
     gene            complement(7066..7533)
                     /locus_tag="VKZ48_07795"
     CDS             complement(7066..7533)
                     /locus_tag="VKZ48_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HLU77788.1"
                     /translation="MPLEPHVSSIASVIQLAVAPVFLLTAIGSLLGVLAGRLARAVDR
                     ARRIEGLLSDQEGDALTQSLHELQVLSRRARWVNRAITACTACALLICLEVAALFIGA
                     FLNTDLSALAGSLFIVAMLTLTLGLISFLREIFLASQHLRIGTHRPGLFWKGH"
     gene            complement(7674..8321)
                     /locus_tag="VKZ48_07800"
     CDS             complement(7674..8321)
                     /locus_tag="VKZ48_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLU77789.1"
                     /translation="MRILLVDDHTLFRAGLRSLLIGMLPDAEVQEAVDGRSALEMVDD
                     RAPDLLISDLSMPDMDGFALLEAVRARQPRLPVLVLSMHADGVFVHRAISLGAAGFLL
                     KDAATEELKLAVEAVARGEVYLSPRAAKQVVAELSRRTEKTSDPATELPARQREVLKR
                     IAEGMSTKEIAYELGLSIKTIEVHRARLMERVGVKDIAGLVRHAIRIGLVKVDME"
     gene            complement(8338..>8935)
                     /locus_tag="VKZ48_07805"
     CDS             complement(8338..>8935)
                     /locus_tag="VKZ48_07805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HLU77790.1"
                     /translation="ARELHDELGQILTVAKMRLGMAAAKDGQPAQELLHEAVRDIDNG
                     IDAVRRMSLDLHPPQLQFGLEAAVSALLHRDIPEDGPCFELYSTLGELKLDRTSAITA
                     FRVVQESLSNALRHAAAQQIDVRLSREDTWLHVVVRDDGAGFDAATAKRKALEGEATG
                     LLGLRERCYSIGGELVVSSAPGAGCEIHAQLPLRHHEI"
BASE COUNT         1524 a         3026 c         2813 g         1572 t
ORIGIN      
        1 gaataagttt gatcggccag tccgtgatcg tccttgatca ggtccacgcc gcccaacgcg
       61 aagcgatagc acaactccgc cagcgcttcg acgggcaggc cctgcggctt gagcgccgca
      121 caggtgagcg ggcgatcgta cgcgttgcac agggcacgca aaccctcgat gccgaagcgg
      181 ggccgggcga agtgtcgtac gaaatccgcc gggaagcgcg cgtcgatcaa ctccacatgc
      241 gcatgcagcg aactattgcc gtaaagcatg ttgagcgtct gcgcgacatc gccgcccatg
      301 gtcgtgacgg aaagcgcaag cgtcacttcg tggtattcgg cgtccagcga acggatggat
      361 tcgacgcgac ccaggacttc gtcgcgcaca aaagcatcgc gcaccacatc gcgcggcgtc
      421 tcgacgcttt gttccagcgc gagcgattca gcgaccgcct cgatgtcggc ggcggcagcg
      481 cgcacgcgat aagtgaccag gacgcgggac attccggaag cagtggaaag caaagccgga
      541 attgtagcgc ccgcaggcgg gggaaacggc gcggcgccga aagcgcgccg cgaggtggaa
      601 ggcttaagcc ttcgacggcg tttgcaactc ggcgtcgcaa cccaccgcct gtacggcccg
      661 gtgcaactgc cagggctgca cggtctcggg atcgaaggcc acgcgcgcct gccgctgcgc
      721 gagggaaatc tgtaccgcgc gcacgccgcg gatgagcgtc agcgcgctcc gtacgctgcg
      781 cgccgcgctt tcgtcatcca cgccggacag cgccagcacg gcggagccgg accggcgcgg
      841 ggccttcatg gcgaagtcgc cgagtttcac gtttccgctt ccggctgcac gaccggccgc
      901 ggaccgcgct ccggcgcatt cgcaccgatc agcatgcgct cggccgcgtc gaaggcatcg
      961 cgcacggcga cataggcgtc ctcgttgcgg atgtcgtcga tcaggatgtt tggaccggga
     1021 acggccagtt cgatcagcac gccgatttcg ttcccgctgc ggcgacgcgc gctgaggcgc
     1081 tccaccacca cgcggccgct ggtaatgcgt tcgtccagcg cctcgagctt tgccatcttc
     1141 tcgcggatcc gcgcttcgag cgccggggac gggtccatgt cgcgaaaggt gatctgcaag
     1201 ggtgttgcca tgacgccgga cctctctcgg tagttcatcg gatgcattca tcctaggcgc
     1261 gtttcgcagc cgatgattgc gccagatcaa gagaatggcc ccggcacccc gccccggccc
     1321 ggctatgccc tgccgatacg atgcactacc attggcggcc ccgtgcggca aagccggcct
     1381 gcgcgccgtg cgctccgcaa gcaacacatg aattcaataa ctcaatcccc cccgcaactt
     1441 tccggctggc gacgcctgct gccttttttg cagtggtggg cgatggtgga ccggcacacc
     1501 ctgcgcgccg atgcgctggc cggactgacg ggcgcgatca ttctggtgcc gcagggcgtg
     1561 gcattcgcca ccatcgccgg catgccgccg gagtacggcc tgtacgcctg catggtgccg
     1621 gccatcatcg ccgccctgtt cggttcgagc tggcatctgg tcaccggccc cagcaccacc
     1681 ggttcgctga tcgtgttcgc ctcgctcagt gcgctggcca tccccggctc gcccgattac
     1741 atccggctgg cgctgacgct cgcttttctg accggcgcct tccaactggc gctgggtctg
     1801 gcccgcatgg gtgtgctggt caatttcgtc tcgcagacgg taatcaccgc ctatgtcgcc
     1861 ggcgccgcgg tatggatttt ctgcagtcag ttgggcaact ttttcgatgt ggccacgccc
     1921 cgcggcatgt cgctcgtcga tctgctgcaa agcttcttta tgcagttggg cgacatcaat
     1981 ccctacaccg ccgccgtggg cgtcgtgacg ctcgcagccg gcctgctgac ccgacggctg
     2041 tggccgcggt tgccgcacat gatcgtcgcc atcgtcgccg gcagcgtctg cgcggtcgcg
     2101 ctcgatcaag tcttcggtca ggacgtcacg aacatccgta ccgtgggcgc catccggcag
     2161 gcgctgccgc cgctgtcgct gcccgatttt tccgccgacg ccatcgaagc gacgctgctg
     2221 agcgcactgg tgctgaccgt gctgacgctc accgaagcgg tgtccattgc gcgcgcggtg
     2281 gcgctgcgca ccgaccaggt catcgacaac aaccagacgt ttatcggtca gggcctggcg
     2341 aacgtggtcg gcgcattctt ctccgcctat ccgtcaagcg gctcgttcaa ccgcacggga
     2401 ctgaactacg aggccggcgc caaaacgcca ttgtccgcca tcctcggcgc ggtgtttctc
     2461 gtgcttattc tggtcgcact ggcgccgctg gcgcgctatc tgccgatcac ctcgatggcg
     2521 gcgctgctgt tcatggtcgc ggtggggctg gtgaatgtcg agcagatccg caaggcatgg
     2581 cacgcgagcc gctgggaagc gctggtgctc gtcgtcacct tcgtcgccac gctggtcgat
     2641 ctgcgcgtaa gcatcttcat cggcatggcg ctgtcgctgg cggtgttcgt ctatcacgct
     2701 tcccgcccca ccgtttccac ggccgtgccg catgccgatc cgaacagtgc gcatttcgtg
     2761 gacggagacg gcatcgtccc cgactgcccc tcgctcaaga tcgtgcgcat caacggcgcg
     2821 ctctatttcg gtgcggccaa ctacatccag caggcgctga tgcgcatcga ccgtgacgat
     2881 ccgcgacacg tcaacgtgct catcgtcgca cgcggcattc actacatcga tgcggccggc
     2941 gcccaggtgc tggcgcagga agcgcgacgg cgacgaaagc tgggcggcgc gctttatttc
     3001 taccgcctca gcgacacggc gcaggagaca ctgcgcagga gcggctacct cgacgacatc
     3061 ggcgaggcga atctctatcc aacccagacg ggcgtcctgg acagcgtgaa ggcggcgatc
     3121 gccgcgcgcg caccgaccgc gtagccggcg cgcagcttgc gaacaccgcg ttgaggcagc
     3181 cctcagccgc cgcccgcgcg acgcagcatg tcgtccactt cgcgttcggc gtgcagccaa
     3241 tcgtccattt ccgcacccgg cgtccagccg cgccattccg cacggaagta ggccaggcgc
     3301 tggatcatgc gatgacgctc ttccggcgaa agctcgcgcg ccggaaccgc atcgcgccca
     3361 tcgacatcgc tttctcttgc tgacacggag ggcgccgcaa cgggcttgcg cggccgccgc
     3421 gccgttccgt tttcgctctt ggcctgagcc ggcgccttga cacgcttcgc ggcggcgcgt
     3481 ttgcgcggcg ccgctgtgcc ctcgccgttc attcttttcg aatccgcggc ggtgccgctt
     3541 tcgatcgtgc gcgtcttgcg cgccggcgcc tcctgctttg cgcgcttgct cgcgcgcgcc
     3601 gcgtccgatt cgcgcttggc ctggcgatcg tcgttcatgc ttgatttctc ccggtgcatc
     3661 gcgtccacct taagcggtcc gcgttacgga aaattgataa ttctcaaccg ccgcgtcctt
     3721 gggcccttca ggctccaggt ctttttccgg aaccctcggt gtgcacgcga atcggccgac
     3781 gccggaccga tgcacgaaat gcgcctgcat acaatggcag tcgaaggtct atattgaaag
     3841 cgaacgggag agaagctgcg cgtcaggaga tcgcagtgcc gaccacgcgc gaagaaagga
     3901 tgcggctgga gcgcatcatc gaatccgcca tggacgcgat catcacggtg gatgatcggc
     3961 agcagatcgt tttatataac cgcgccgccg cgatgatgtt cggcgtgcct ccggacgatg
     4021 cgctcggagg cacactggaa cgtttcattc cggaccgcta tcggcgcgca cgaatttcac
     4081 ccatcatgca tttcggcgcc gcgccgcccg gcatggcgga gcggcgcacg ggcggccgca
     4141 cggtctatgg ccgccgcgcc aacggcgaag agtttccgat ggatgcgtcg atctcggaca
     4201 tcgacgtggg cgaccgccgc ttctacaccg tcatcctccg cgatatcacc gatcgcgtcc
     4261 gtgcggaaca ggcgctgaag caggcgcaca cggaactgac gacgctgtca cgcgcggtcg
     4321 gcacgtcgct ggagaatcag cgccgtcgca tcgcgcgaga actgcatgat gaactgggcc
     4381 agatgctgac ggcgatgaaa atcgacgtta gcgaactgca gggtcagttg cccaaaggcg
     4441 ctgccgatct gcgcgagcgg tgcgaacagt tgcgcctgct gatcgatcag accattgccg
     4501 ccacccgccg catcgcaacc gacctgcgtc cgctcgtgct ggacgacctg ggcctcgccg
     4561 cggcgatcga ctggctggcg gaaaatctgc gcaagcgcac aggcctgcac atcagcgtgc
     4621 gattcgatga ggcaatgacg catgtcggcg aaccctatgc ctcgaaccta ttccggatca
     4681 tccaggaatc gttgaccaac atcgtgcgcc acgccggggc gacggaggcg cagatcgagg
     4741 tttcgcacga cggcgtcgag gcacgcctca tggtttgcga caacggccgc ggcattcgca
     4801 ccgacgaaac gcagcgtcac tgtctcggcc tgctcggtat ccgcgaacgc gcccggatac
     4861 tcggcggcag cgcgaccatc gcgccgcgca ccgggcgcgg gacgcaactc gtcgtaacct
     4921 tcccttaccg ccccggacag gaggccgcct gatgatccgc atcatcatcg ccgacgacca
     4981 cgccattgtg cgcgacggcc tgaagcgcat catcgaaacg gcgcccggct gcgaggtggt
     5041 gagcgaagcg gtcgacggcg cggaagtgat cgatcgggta cgcacgcaac cggccgatgt
     5101 gctgctgctg gatctgtcca tgccgggcgc cagcggcatc gatctcatcc gtcacgtcaa
     5161 aagcatcgcg ccgccgctgc acatcctggt gctgtcgatg cacgcggaag agcagtacgc
     5221 cttgcgctcc atacgcgccg gcgcctcggg ctatctcacc aaggactgcg cgtccgacag
     5281 cctggtgcgt gcactgcgca aggtcgcctc cggcagcgtc tacatcagcg agggcgtcgc
     5341 cgagcagttg gcccgccatc tcaatcttgg cgcaatggac gaggtgcccc atcgcagcct
     5401 cagcgaccgc gaatacgaag tctttctggc gctggtgtcg gggcgctcgg tgtccgatat
     5461 cgcgcacgcg ctcaacctct cggtcaaaac catcagcacg cataaaagcc gcatcctcga
     5521 aaaaatgcag ttggatagcg tggcggatct ggtgcgctac gccgttgcgc atcgattgat
     5581 cgacctgccg tatccgaata tgccctcgca gtagtctgcc ggacaacgct gcgcaggaca
     5641 aaagtagtta atgcccgaca aggggtagga cgattcctac ggcactacga ttgcgccgag
     5701 aaaaaaatcg tcctcggccc aatagccggt gcggggccgt atcgggcaca gtcgcccggt
     5761 gccccaagaa ctgctgcccc ctctcgctgc ctcgcccgac ctgcgcgtct tcattgcgga
     5821 acgttcagcc catatacgcg cacgcatggt gtccctgctg gaccggtgtg cgggcgtggc
     5881 ctacgtgggt tgggcggaga gcgtgggcga atgcatcgcc ggcgtccggc gcacgaatcc
     5941 ccacggcctg gtcctcgatc tccagctcgt cgatggctgc gcgctaccgg cactgcgagc
     6001 gttgcgcagc gacaaccccg gcctgcgcgt cgtggtgtgc gcgacctggg ccaccgcaca
     6061 gcacgaggcc gcctgccgcg aggcgggcgc cgaacgcctg atcgacaaag ccaccgagtt
     6121 ccccgagttg agcaacatcg tgcaggcctg ggtgcgttcg cacggggtcc ggccggtcat
     6181 tcattaaacc ccaagatatc gaaaccttgc tacgcagcct ttgaaggagt cgtcatgcct
     6241 gtcatcgcat tacgcgaacc caatccgagt ctcaccatgc agcggcggtc gcacgccgcc
     6301 gggtacgtga tattgcgcga gttgaacggg ctgttgcatc tggatttgcc tgaatgcgcg
     6361 gcactcgacc gcctggactt tgccacgcgc cagttgcgcg ccggcgagtc gctggtgcat
     6421 tgcggcgacg aatgccgcgc gctgtatgcc gtgcgtttcg gcagcctgcg cagtgcgatg
     6481 atcgacgcgg acggcgccga gcaggggctg ggcttcccca tgcccggtga catcgtcggc
     6541 accgacgggc tcgccaccgg acgacacacc gcgcatgcgg tcgcgctgga gcgcagcgag
     6601 gtagtcatta tcccgctcgc ccgcatcgca gaaatcgcgc gcacactgcc gggcttcgac
     6661 ctcgtgctct atcgcatcat cggccgggaa accctgcgcg atcacgccac gctctatctg
     6721 ctcggttcgc tcaacgccga agggcgcgtc gcagccttcc tgctgaacct gtccgagcgc
     6781 tacggcgaac tgggctatgc gcgcaacgtc ttcaacctgc gcatgacgcg caacgacatc
     6841 gccagctacc tgggcctaac gctggagacc gtgagccgcg ccttctccgc ctatgcctca
     6901 tgcgatctgc tgcgcgtgga cggtcgtgaa atcgaaattc tcgaccgcga agcgctccag
     6961 cgcgcgatca ccgcgcctag tgcggtgctg cgcggcggac gcgcggcgcg tccgcaatcg
     7021 ggccgttacg gcggcgcact cgcgctggct agttgagcag cgagcttaat gccccttcca
     7081 gaacaggccg gggcgatgcg tgccgatgcg cagatgctgc gaggccagaa aaatctcgcg
     7141 caggaaggaa atcagcccga gcgtgagcgt cagcatcgcg acgatgaaca ggctgcccgc
     7201 gagtgcggac agatcggtat tgagaaaggc gccgataaag agcgccgcca cctccagaca
     7261 gatcagcagc gcgcacgccg tgcaggcagt aatggcgcga ttgacccaac gcgcgcgacg
     7321 cgagagcact tgaagttcgt gcagcgactg ggtgagcgca tcgccttcct ggtcgctcaa
     7381 cagcccttcg attcgccgcg cacggtccac tgcgcgcgcc agccggccgg ccagcacgcc
     7441 cagcagggag ccgatcgcgg tcagcagaaa gactggtgca accgccaact ggatgacact
     7501 ggcaatgctc gatacatgcg gttccagcgg cattgcgctt cccccttttt taattggcct
     7561 tggacgcgtg ctcatcataa aggagcgccg cgtcccattc agccgaatct tgccgcttgc
     7621 agtgttcgcc ccccaggacc ttgctgtggc ggcgcaggtg cccggccagc gcgctactcc
     7681 atatcgacct tgaccaggcc gatgcggatg gcatggcgca ccagcccggc gatatccttc
     7741 acgccgacgc gctccatcag acgcgcccga tggacttcga tggtcttgat gctcaagccc
     7801 agttcgtagg cgatttcctt ggtgctcatg ccctcggcga tgcgtttgag cacttcacgc
     7861 tggcgcgcgg gcaactcggt ggccgggtcg gatgtctttt cggtacgtcg cgacaattcg
     7921 gcgacgacct gcttggcggc acgcggactg agatagacct cgccgcgcgc gaccgcctcg
     7981 accgcgagct tcagttcctc ggtcgccgca tccttgagca ggaagccggc cgcgcccagc
     8041 gaaatggcgc ggtgcacgaa gacgccgtcg gcatgcatgg acaacaccag caccggcagg
     8101 cgcggctggc gcgcgcgcac cgcctcgagc aaggcgaaac cgtccatatc gggcatgctc
     8161 aagtcgctga tcagcagatc gggtgcacgg tcgtcgacca tttccagcgc ggagcggccg
     8221 tcgaccgcct cctgcacttc ggcatcgggc agcatgccga tcagcaggct gcgcaatccg
     8281 gcgcgaaaca gcgtgtgatc gtccaccagc aggatacgca tagcgtgtag gtgctttcta
     8341 tatttcgtga tggcgcagcg gcaactgcgc gtggatttca cagcctgcgc cgggcgcgga
     8401 agatacaacc agttcgccgc cgatgctata gcagcgctcc cgcaagccga gcagccccgt
     8461 cgcttcgcct tcgagcgcct tgcgtttggc cgtcgcggca tcgaagcccg ccccgtcgtc
     8521 gcgcacgacg acatgcagcc acgtgtcctc cctggaaagg cggacgtcga tctgctgcgc
     8581 ggcggcgtgt cgcagcgcat tgctgagcga ctcctgcacc actcgaaaag cggtgatggc
     8641 gctggtgcga tccaatttca attcgccgag cgtcgaatac agttcgaagc acggcccgtc
     8701 ctcggggatg tcgcgatgca gcaaggcgct caccgccgcc tccaggccga actgcaattg
     8761 cggcggatgc agatcgagcg acatgcgccg caccgcgtcg atgccgttgt cgatatcgcg
     8821 cacggcctcg tgcagcaact cctgcgccgg ctgcccgtcc ttagcggcgg ccatgcccag
     8881 gcgcatttta gccacggtca ggatctgtcc gagttcatcg tgcagttcgc gcgcg
//
LOCUS       DATGOU010000133         3274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_5735877, whole genome shotgun sequence.
ACCESSION   DATGOU010000133 DATGOU010000000
VERSION     DATGOU010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5735877"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="VKZ48_07810"
     CDS             <1..941
                     /locus_tag="VKZ48_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional glutamine synthetase
                     adenylyltransferase/deadenyltransferase"
                     /protein_id="HLU77791.1"
                     /translation="RELHHAQVFRLLAQDLAGLLSVERLADHLSIAADLVLTVAVEEC
                     WKLVRQRHRETPRIALIGYGKLGGKELGYASDLDLVYIYDDDHPAAPENYARLVQRIT
                     TWLSSHTAAGVLFETDLRLRPNGEAGLLVHSMAALRRYQTESAWVWEHQALTRARFCA
                     GDAGLGALFEALRRDILTAPVDRAALRADVLAMRAKMAEGHPNRSDLFDLKHDRGGMV
                     DIEFIVQYLVLAYAPRHYELTANLGNIALLHMAGGLQLIPAALATTVADAYREYRRLQ
                     HKLRLNGAEYARVEAETVAEHIEATLALWRTVFEAD"
     gene            1062..1988
                     /locus_tag="VKZ48_07815"
     CDS             1062..1988
                     /locus_tag="VKZ48_07815"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015065043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HLU77792.1"
                     /translation="MSMADRDGWIWYDGKLVPWRDATTHVLTHSLHYGLAIFEGVRAY
                     NTVEGTAIFRLQEHTERFFNSAHIYRMKIPFTAEELNEAQKEVVRANKLESAYIRPIA
                     FYGAEKMGVSPIGAKVHVSIAAWPWGAYLGAEGLEQGIRVKTSSHARHHVNVSMCRAK
                     YSGTYANSILANMEATEHGYDEGLLLDVDGFVAEGPGENLFIVKKGCIYEPEIASALM
                     GITRDTLITLAADLGYEVKSRRLTRDDVYIADEAFFTGTAAEVTPIREVDGRTIGEGK
                     RGPVTTKLQKAFFDLVAGKDKKHAEWLTPVGA"
     gene            2023..2223
                     /locus_tag="VKZ48_07820"
     CDS             2023..2223
                     /locus_tag="VKZ48_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HLU77793.1"
                     /translation="MAEAQQKETIVEVTEKDLPLHCPTPNTPVWNYHPRVFLDVVDLG
                     TVRCPYCGTLYEYKGAPITGGH"
     gene            2310..>3274
                     /gene="waaF"
                     /locus_tag="VKZ48_07825"
     CDS             2310..>3274
                     /gene="waaF"
                     /locus_tag="VKZ48_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018313663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HLU77794.1"
                     /translation="MSRTLVVAPNWIGDALLAQPLFSRLREARPGERIDALAPPWTAP
                     VLGRMPEIDEVIQVPFNHGELKIAARWRLGRRLKERGFSRAVVLPNSWKSALVPFFAG
                     IPERIGFVGEARYGLLNVVHKLDEKALPLMAERYAKLAEDPSEPPQRPLPRVRLRVDE
                     ANLIITIGRLGLDRSRPVIALCPGAEYGPAKRWPTRHFAALARQLQARGNAVWLFGSE
                     KDRPVADEIVALSDDACVNLCGRTNLAAAIDLMSVARCVVSNDSGLMHIAAALGRPIV
                     ALYGSSSPTHTPPLSRNARLVTLNVECSPCYQRECPLGHFKCMNDL"
BASE COUNT          603 a         1117 c         1002 g          552 t
ORIGIN      
        1 tgcgcgaact gcatcacgcg caggtattcc gcctgctcgc ccaggatctg gccgggctgc
       61 tgtcggtgga acggctggcc gatcatctgt ccatcgccgc cgatctggtg ctcactgtcg
      121 cggtggagga gtgctggaag ctggtgcgcc agcggcatcg cgaaacgccg cgcatcgcgt
      181 tgatcggcta cggcaagctg ggcggcaagg aactgggcta cgcctccgac ctggacctcg
      241 tctatatcta cgacgacgac cacccggccg cgccggagaa ctacgcgcgc ctggtgcaac
      301 gcatcaccac ctggctgtcg agccacacgg ccgccggcgt gctgttcgag acggatctgc
      361 gtctgcggcc caacggcgaa gcaggcctgc tggtgcattc catggccgcg ctgcgccgct
      421 atcagaccga atcggcctgg gtgtgggaac accaggcgct cacgcgcgcg cgtttttgcg
      481 ccggcgatgc cgggctgggc gcgctgttcg aagcgctgcg gcgcgacatc ctcaccgcgc
      541 ccgtggaccg ggccgccctg cgcgccgacg tgctcgccat gcgcgccaaa atggccgaag
      601 gccatcccaa ccgctccgat ctgttcgatc tcaagcatga ccgcgggggc atggtggata
      661 tcgaattcat cgtgcaatac ctggtgctcg cctatgcgcc gcggcattac gaactgaccg
      721 ccaacctggg caatatcgcg ctgctgcaca tggcgggcgg tctgcaactc attcccgcag
      781 cgctggcgac cacggtggcc gacgcctacc gcgaatatcg ccggctgcag cacaaactgc
      841 ggctgaacgg tgccgaatac gcccgtgtcg aggccgaaac ggtcgccgaa cacatcgagg
      901 ccacgctcgc gctgtggcgt acggtattcg aagcggattg agcgacccga ccgctgacaa
      961 tgcggaaggc gtcgcttttt ggctagaatg ccgcctttgt ttcagctcgg atgcaccgcc
     1021 cgacgcggcg attgcaccga cccattccgg agaacaacac aatgtccatg gccgaccgcg
     1081 acggttggat ctggtacgac ggcaagctcg tgccctggcg tgatgccacg acccatgtcc
     1141 tcactcactc gctgcactac ggcctggcca tattcgaggg cgtgcgtgcc tacaacactg
     1201 tcgaaggcac ggcgattttc cggctgcagg aacacacgga gcgctttttc aattccgcgc
     1261 acatctaccg gatgaaaatc ccgttcacgg ccgaagaact caacgaggcg cagaaggaag
     1321 tggtgcgcgc caacaaactg gaaagcgcct atatccgccc catcgccttc tacggcgcgg
     1381 aaaagatggg cgtttcgccc atcggcgcaa aagtgcacgt ctccatcgcc gcatggccgt
     1441 ggggcgcgta tctgggcgca gagggcctcg aacagggcat ccgcgtgaag acctcctcgc
     1501 acgcgcgcca tcatgtcaac gtatcgatgt gccgcgccaa atactccggc acctacgcca
     1561 actcgatcct cgccaacatg gaagccaccg aacatggcta cgacgagggt ctgctgctcg
     1621 acgtggacgg attcgtcgcc gaaggtccgg gcgagaacct gttcatcgtc aagaaaggct
     1681 gtatctacga gccggagatc gcctccgcgc tgatgggcat cacgcgcgac acgctcatca
     1741 cgcttgcggc ggatctcggt tatgaagtga aatcgcgccg cctgacgcgc gacgacgttt
     1801 atatcgccga cgaagccttc ttcaccggca ccgcggccga agttacgccc atccgcgaag
     1861 tggacggacg caccatcggc gagggcaagc gcggtccggt cacgaccaag ctgcagaagg
     1921 ccttcttcga cctggtcgcc ggcaaggaca aaaaacacgc ggagtggctg accccggtcg
     1981 gtgcctgacc gcgagccgca tttcagacgg agcaaaccaa aaatggctga agcccaacaa
     2041 aaggaaacca tcgtcgaggt caccgagaag gacctgccgc tgcactgtcc cacacccaac
     2101 acgccggtgt ggaattacca tccgcgcgta ttcctcgacg tggtggatct cggtacggtg
     2161 cgttgcccct actgcggtac cttgtacgaa tacaagggtg cgcccatcac cggcggccac
     2221 tgagcccgcc ccgtcgtcga cgcgtgccga cgacgaacaa accgggcacg ttgctgtctt
     2281 ttctttaacg cgcccgccca cgacccgcta tgtcacgcac cctcgtcgtc gcacccaatt
     2341 ggatcggtga tgcgctgctg gcgcaaccgc tgttctcgcg cctgcgcgaa gcgcggcccg
     2401 gcgagcgcat cgacgcgctc gcgccgccat ggaccgcgcc ggtgctgggc cgcatgcccg
     2461 agatcgacga agtcatccag gtgcccttca atcacggcga actgaaaatc gccgcccgct
     2521 ggcgactagg ccgccgactc aaggagcgcg gcttctcacg cgcagtggta ctgcccaaca
     2581 gttggaagtc ggcgctcgtg cctttttttg ccggcattcc cgaacgcatc ggtttcgtcg
     2641 gcgaagcgcg ttacggcctg ctcaatgtcg tgcacaaact cgacgaaaag gcgctgccgc
     2701 tgatggccga gcgctatgcc aagctcgccg aagacccgtc cgagccgccg cagcgcccgt
     2761 tgccccgcgt gcggctgcgc gtggacgaag ccaatctcat cattaccatc ggccgcctcg
     2821 gactggatcg ctcgcgtccg gtgatcgcgc tctgcccggg cgcggaatac ggcccggcca
     2881 agcgctggcc cacgcgccat ttcgctgcgc ttgcgcggca gttgcaggcg cgcggcaacg
     2941 cggtgtggct gttcggctcg gaaaaggatc ggcccgtcgc cgatgaaatc gtcgcgctgt
     3001 ccgacgacgc ctgcgtcaac ctgtgcggcc gcaccaacct cgccgccgcc atcgacctga
     3061 tgtcggtcgc ccgatgcgtg gtcagcaacg attcgggcct catgcacatc gccgccgcgc
     3121 ttgggcgacc gatcgtggcg ctctacggct cctccagccc gacgcacacg ccgccgctca
     3181 gcaggaacgc gcggctggtg acgctcaatg tggaatgcag tccctgctac cagcgcgagt
     3241 gtccgctggg tcacttcaag tgcatgaacg atct
//
LOCUS       DATGOU010000134         3321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_4395036, whole genome shotgun sequence.
ACCESSION   DATGOU010000134 DATGOU010000000
VERSION     DATGOU010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4395036"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VKZ48_07830"
     CDS             <1..455
                     /locus_tag="VKZ48_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HLU77795.1"
                     /translation="SGFLGDLLAEVEFVAVARPDHPLHRLGRELSVDDLAREQQIVIR
                     DSGTRHPRDAGWLGAERRWTVTGVETSISLVAGGLGFAWLPCHLIQPYLDAGTLEPLP
                     LREGQRRRAPLYLIFAQPELAGPATRELAQVLRDAAARADDGSQPHRA"
     gene            531..1484
                     /locus_tag="VKZ48_07835"
     CDS             531..1484
                     /locus_tag="VKZ48_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HLU77796.1"
                     /translation="MADFRAGTGDTLEVATAETSMIDLLIPQRRRSLGGFEVGRVLPY
                     MQRRMIGPFIFFDHMGPVDFPPGVPESTDVRPHPHIGLSTITYLLEGEIMHRDSVGSV
                     QPIRPGEVNWMTAGRGITHSERFERARREGGPMNGLQAWIALPREYEEIEPSFTHYAE
                     EQLPILDEAGVHLRVIAGEAYGAKAPVAVHSPLFYLHAELRAGASVGLPAGYPERAAY
                     VVTGAAEVGAERLAAGEMAVFRPGADVRIKALEAAHIMLLGGEPIGPRYIDWNFVSSS
                     RERIEQAKADWRAGRMKLPDFDGDDFIPLPPEPVAPPDPMS"
     gene            complement(1514..>3321)
                     /locus_tag="VKZ48_07840"
     CDS             complement(1514..>3321)
                     /locus_tag="VKZ48_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HLU77797.1"
                     /translation="SAAARIGRNDFSAQAMVRGDNEFATLAGAFNTMSDTIGRQLATL
                     HRYAEIDRIVLTTLDMRAVAEKALQQIRLLTDAAVVVLQFEDEYGVHCFRLDTAGMHS
                     SQGEASAASEGTLTRHYDIRHRDERLGCLAIHEPRVADDAAELAVGGYLDRIAIALAA
                     AQRERRLVRQARMDSLTGLPNRAYFLEHLQQAVGDAVTHSERGTLLFLDLDRFKRTND
                     VLGHVAGDTLLKLAADRLNDTVGAEHFLARLGGDEFTVVCRRTHDPQVIEALATRLIE
                     RLSEPFEIDGRTIFVGLSIGSATFPDDGDTALDLLRRADTAMYRAKQRGGARYAAFHT
                     DMEGDLSRSLTMDAALREAITQGRFELYYQAKADMQSGRVSGAEALIRWIDPDGMVLD
                     TPAWMAHAETTPMINEIGAWALTRACSQLREWDEAGWRLPRVAVNVAARQLTDSGFYE
                     DVVNAISTAGIAPHRLQLEITESQLMTDPAVSLPLLRRLTALGVTIAADDFGTGYSSF
                     SLLRQLPLSVLKVDASFIADIDADPSARAVVQAIVRMGHALQLRVVAEGVETVAQRKV
                     LAEIGCDTVQGYLIGRPIPAYAFMLAYQDSLAVEH"
BASE COUNT          558 a         1061 c         1094 g          608 t
ORIGIN      
        1 cgtccgggtt cctaggcgat ttgctggcgg aggtggagtt cgtcgccgtg gcgcggccgg
       61 atcatccgct gcatcgactc ggtcgggaat tgagcgtgga cgatcttgcg cgcgaacagc
      121 agatcgtcat ccgcgactcg ggtacgcgtc atccccgcga tgcgggttgg ctgggcgccg
      181 aacgccgctg gacggtgacg ggtgtcgaaa cttcgatctc actggtcgcc ggcggattgg
      241 gctttgcctg gctgccttgc cacctgatcc agccgtatct cgatgcgggc acgctcgagc
      301 cgctgcccct gcgcgaaggg cagcgccgtc gcgctccgct gtacctgatt ttcgcgcagc
      361 ccgaactggc cgggccggcg acgcgtgagc tggcgcaggt gctgcgcgat gcggctgcgc
      421 gcgcagacga cgggtcgcag ccccaccgcg cctgatgttt gcgttcgtcg aaggccattg
      481 aaccccgggt tcccggccgg ataaggcaat cggcaaggcc tttgcgccgc atggccgatt
      541 tccgggccgg gacgggcgat actctcgagg tcgccacagc ggaaacgtcc atgatcgatc
      601 tgctcattcc ccaacgccga cgcagcctgg gcgggttcga agtcgggcgg gtattgccgt
      661 atatgcagcg ccggatgatc ggccccttca tctttttcga tcacatgggg ccggtcgatt
      721 tcccgcccgg cgttcccgaa tcgacggatg tgcgtccgca cccgcatatc gggttgtcga
      781 ccatcactta tctgctggaa ggcgaaatca tgcatcggga cagcgtgggc tcggtgcagc
      841 ccatccgacc cggcgaagtc aactggatga ccgcgggccg cggtatcacg cattccgaac
      901 gcttcgaacg cgcgcgccgc gaaggcgggc cgatgaacgg gttgcaggcc tggattgctc
      961 tgccgcggga atacgaggag atcgaaccgt cgttcaccca ctatgccgag gagcagttgc
     1021 cgatcctcga cgaggcgggt gtgcatttgc gcgtcatcgc cggtgaggcc tacggcgcca
     1081 aagcgccggt cgcggtgcac tcgccattgt tctatttgca tgccgaactg cgggccggcg
     1141 cgagcgtggg tttgccggcc ggttatccgg agcgtgccgc atatgtcgta acgggagcgg
     1201 cggaggtggg tgccgagcgc ctggccgccg gcgagatggc ggtattccgc cccggcgccg
     1261 acgtgcggat caaggcattg gaggcggcgc acatcatgct gctgggcggc gaaccgatcg
     1321 gtccgcgcta tatcgactgg aacttcgtgt cctcgtccag ggaacgcatc gagcaggcga
     1381 aggccgactg gcgcgccggt cgcatgaaac tgccggactt cgacggcgac gacttcattc
     1441 ctctgccgcc ggagccggtt gctcctcccg atccgatgag ctgagcggag gtgcctgata
     1501 cccgcctgaa ggcctaatgt tcgacggcga ggctgtcctg ataggccagc atgaaagcgt
     1561 aagccggtat cggtctgccg atcaggtaac cctgcacggt atcgcagccg atttcagcga
     1621 gaacctttcg ctgagcgacg gtttcgacac cttcggccac cacccgtaac tgcagtgcat
     1681 gtcccatccg tacgatcgct tgtaccacgg cgcgcgcgct cgggtcggca tcgatatcgg
     1741 cgatgaaact ggcgtcgacc ttcaacacgg acaaggggag ctggcgcaac aggctgaagg
     1801 aggagtagcc ggtgccgaaa tcgtccgcgg cgatggtcac gcccagcgcc gtcaggcgcc
     1861 gcagcagcgg aagcgaaacg gccgggtcgg tcatcaactg cgactcggtg atctcgagct
     1921 gcaatcgatg cggtgcgatg ccggctgttg aaatggcgtt gacgacgtcc tcgtaaaagc
     1981 cactgtccgt caattgccgc gcggcgacgt tcaccgcaac gcgcggaagc cgccagcctg
     2041 cttcgtccca ctcgcgcaac tggctgcatg cgcgcgtaag cgcccaggcg ccgatttcat
     2101 tgatcatcgg tgttgtctcc gcatgcgcca tccacgccgg cgtgtcgagc accatgccat
     2161 ccggatcgat ccagcggatc aatgcctcgg cgccacttac gcggccgctt tgcatgtcgg
     2221 ccttcgcctg atagtagagc tcgaagcggc cctgcgtgat cgcctcgcgc agggccgcgt
     2281 ccatcgtcag actgcggctg agatcgccct ccatgtcggt atggaacgcg gcatagcgcg
     2341 cgccgccgcg ttgcttggcg cgatacatcg ccgtgtcggc gcggcgcagc aaatcgagtg
     2401 cggtgtcgcc gtcatcgggg aaagttgcgc tgccgatgct cagtccgaca aagatcgtgc
     2461 ggccgtcgat ttcgaaaggc tccgaaagcc gctcgatcag tcgcgtcgcc agcgcctcga
     2521 tgacttgcgg gtcgtgcgtc cgccggcaca ccacagtgaa ttcgtcgccg cccagccgcg
     2581 ccagaaaatg ttccgcaccg acggtgtcgt tcaggcggtc cgcagcaagc ttcaacagcg
     2641 tgtcgcccgc gacatgaccg agcacatcgt tggtgcgctt gaagcggtcg agatccagga
     2701 aaagcagcgt gccgcgttcg ctgtgcgtga ccgcgtcacc cacggcttgc tgcaggtgct
     2761 cgagaaaata cgcgcgattc ggcaatccgg tgagcgaatc catgcgcgcc tgccgcacca
     2821 ggcgacgttc ccgttgtgcc gcggcgagcg caatggcgat acggtccagg tagccgccga
     2881 cggccagttc agccgcatcg tccgcgacgc gcggttcatg gatcgcgagg cagccaagcc
     2941 gttcgtcacg atgccggatg tcgtaatgcc gcgtgagcgt cccttcactc gccgcggacg
     3001 cttcgccttg agacgaatgc atgccggctg tgtcgagtct gaagcaatgg acgccgtatt
     3061 cgtcttcgaa ttgcaggacg acaacggcgg catcggtgag caggcggatc tgctgcagcg
     3121 ctttctccgc taccgcccgc atatccagcg tcgtgagcac gatgcgatcg atttcggcgt
     3181 agcgatgcag cgtggccaac tgcctgccga tcgtgtcgct catagtgttg aacgcgccgg
     3241 ccagcgtcgc gaattcatta tcgccgcgca ccattgcctg agccgagaaa tcattgcgtc
     3301 cgatgcgcgc ggcggcggat c
//
LOCUS       DATGOU010000135         2477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3095363, whole genome shotgun sequence.
ACCESSION   DATGOU010000135 DATGOU010000000
VERSION     DATGOU010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3095363"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VKZ48_07845"
     CDS             complement(<1..260)
                     /locus_tag="VKZ48_07845"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLU77798.1"
                     /translation="MRRKVLDGYQIVERMPTPDEYGTIVRAVGWEPVLNFDAVPESLR
                     RSLFGVVATYEGQVIGMGRIVGDGAMYFYIQDIAVLPEHQHRG"
     gene            complement(276..1853)
                     /locus_tag="VKZ48_07850"
     CDS             complement(276..1853)
                     /locus_tag="VKZ48_07850"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HLU77799.1"
                     /translation="MLLFTNGIIRTMDPAAPVVEAVLVGDDGRIAATGRAADLDKPGV
                     RRVDLGGATLLPGFNDAHVHIWKLGLLLTVQVDARSAVAPDIDTIVARFRERAATLPP
                     GTWITGRGYNETALPERRHLTRADLDAASTEHPIALTRTCGHMIVANSKALELAGITA
                     QTPNPPGGVIVRDEAGEPTGLLQETAMGLLNRAMPEIDDATMADAIRATMRHQLALGI
                     TSATDPLLTPSILSVYRDLEARGELVVRVNGLPIRRPDGGTETLPLPERFVSDTLRID
                     CVKFFADGGLSGATAALSEPYKVTGDCGVLRFDTDELTQLMWEAHDAGFRIATHAIGD
                     VALDQVIGVYEALHRRKPQPGLRHRIEHLGLPSAEHLRRCKALDIIAVPQTIFIGALG
                     NAFRRYLPDRFLSRCYPVRAMLDAGLTVALSSDAPVVADDNPLAGLKAAVDRIDPTGE
                     LLAPEQAITREEALYAYTMGGALASGDADNRGSITPGKWADLTVLNGDPLTVPLQDLL
                     ELKVQQTYVGGRLVYEA"
     gene            complement(1864..>2477)
                     /locus_tag="VKZ48_07855"
     CDS             complement(1864..>2477)
                     /locus_tag="VKZ48_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLU77800.1"
                     /translation="RELCDEHGIVMVADEVQTGFGRTGRLFAVEHYGIVPDLIASAKS
                     LAAGMPLSAVTGRAEIMDGPHPGGLGGTYSGNPVACAAAIEAIEMIRQPEFLRRACEI
                     GDRIHQHLLNLKAESTLIGDVRGLGPMQVMELVTDREAKTPAMQETLQVTLEALKRGL
                     IVIRAGLYSNCIRFMPPLNLTDDELDEGMAVVAEAVRAVEAAR"
BASE COUNT          433 a          829 c          776 g          439 t
ORIGIN      
        1 ccccgatgct ggtgctccgg aagaacggcg atgtcctgta tatagaagta catcgcgccg
       61 tcgccgacaa tacggcccat gccgatcacc tgcccttcat aggtcgcgac aacgccgaac
      121 agcgagcggc gcaaagactc agggacggca tcgaaattga gcaccggctc ccagccgacc
      181 gctcgcacaa tcgtgccgta ctcgtcggga gttggcatgc gctcaacaat ctgatagccg
      241 tccagcacct tgcgcctcac tttcctgcgt gcgtgtcacg cctcgtagac cagccgcccg
      301 ccaacgtaag tctgctgcac cttcaattct aacaagtcct gcaacggaac cgtgagcggg
      361 tcgccgttta gcacggtcag gtcggcccac ttgcccggcg taatgctgcc ccggttatcc
      421 gcatcgccgc tcgccagcgc gccgcccatc gtgtaggcgt agagcgcttc ctcacgcgtg
      481 atcgcctgct ccggcgcgag aagttccccg gtcgggtcga tccggtcaac tgccgccttc
      541 aggccggcca gcggattatc gtcggcgacg accggcgcgt cggaactgag cgcgaccgtt
      601 agcccggcgt cgagcatggc gcgcaccgga tagcagcgcg agaggaaacg gtcgggcagg
      661 tagcgccgga aagcgttgcc cagcgcgccg ataaagatcg tctgcggcac ggcgatgatg
      721 tcaagcgcct tgcagcgtcg gagatgctcg gcgctcggca ggccaagatg ttcaatacga
      781 tggcgcagtc ccggctgtgg cttgcggcgg tgcaacgctt cgtaaacgcc gatcacctga
      841 tcgagcgcga catcgcctat cgcgtgggtc gcaatgcgaa acccggcgtc gtgcgcctcc
      901 cacatcagtt gtgtcagctc atcggtgtca aagcgtagca cgccgcagtc acccgtcacc
      961 ttgtacggct cgctcagggc agcggtcgcc ccgctcagcc cgccgtcggc gaagaatttc
     1021 acacagtcga tccgcaacgt atcgctgacg aaacgttcgg gcaacggcaa ggtttccgta
     1081 ccgccatcgg ggcgacggat cggcaggccg ttcacccgca caaccagctc tccgcgcgct
     1141 tccagatcgc ggtaaacgga cagaatcgaa ggggttagca gcgggtcggt cgcgctggtg
     1201 atgccgagcg caagctggtg gcgcatggtc gcgcgaatgg cgtccgccat cgtcgcgtcg
     1261 tcaatctccg gcatggcgcg gttcagcagg cccatcgccg tttcctgcag cagcccggtc
     1321 ggttcgccag cctcgtcgcg cacgatgacg ccgccgggcg ggttcggcgt ctgcgccgta
     1381 atccccgcca gttcgagcgc cttgctgttg gcgacgatca tatggccgca ggtgcgcgtc
     1441 agcgcgatag gatgctccgt gctggcagcg tcgaggtcgg cgcgggtaag gtgtcgccgt
     1501 tcgggcaagg ccgtttcgtt gtagccacgc cctgtgatcc acgtccccgg cggcagggtc
     1561 gccgctctct cccgaaatcg cgcaacgatc gtatcgatgt cgggcgcaac ggcagaacgc
     1621 gcgtccacct gcactgtcag caacaagccg agtttccaga tgtggacatg cgcgtcgttg
     1681 aaaccgggca gcagcgtcgc gccgccaagg tcaacgcgac gcactcccgg tttatcgagg
     1741 tcggcagccc ggcctgtcgc cgcaatcctg ccgtcatcgc ctaccagcac cgcctcgacg
     1801 acgggagcgg cgggatccat cgtgcgaata atgccgttcg tgaacagcag catttgtacc
     1861 gtcctaacgc gctgcttcga ccgcgcggac tgcttcagcg acgaccgcca tgccttcgtc
     1921 cagttcgtcg tcggtcaggt tgagcggcgg catgaagcgg atgcaattgc tgtagaggcc
     1981 agcgcgaatg acgatcagcc cgcgtttgag cgcctcaagc gtcacctgca acgtttcctg
     2041 catggccgga gttttggctt cgcggtcggt caccagttcc atcacctgca tcggccctag
     2101 cccgcgtaca tcgccgatca gcgtgctttc ggccttgagg ttgagcaaat gctggtgaat
     2161 gcggtcgcca atctcacagg cgcggcgcag aaattcgggc tggcggatca tctcaatcgc
     2221 ttcgatggcg gcagcgcagg caaccggatt gccgctgtac gtgccgccca gcccgccggg
     2281 atgcggcccg tccatgatct cggcgcgccc tgtcacagcg gaaagcggca tccccgccgc
     2341 gagcgacttg gcgctggcga tcagatcggg cacgatgccg taatgctcga cggcaaacag
     2401 gcgtccggtg cgtccaaagc cggtctgcac ttcgtccgcg accatgacga tcccgtgctc
     2461 gtcgcacagc tcgcgga
//
LOCUS       DATGOU010000136        11583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3098425, whole genome shotgun sequence.
ACCESSION   DATGOU010000136 DATGOU010000000
VERSION     DATGOU010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11583
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3098425"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /gene="flgG"
                     /locus_tag="VKZ48_07860"
     CDS             <1..654
                     /gene="flgG"
                     /locus_tag="VKZ48_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HLU77801.1"
                     /translation="LLYQTVRQPGAQSSQQTTVPAGLQIGTGAKSVANVRIHTQGNLQ
                     LTGNALDVAINGQGFLQILLPDGTTAYTRDGSFHVDANGQVVNANGYPLQPAVTIPPN
                     ASNITIGSDGTVTVMQPGQVNPVQVGQIQLATFVNPAGLHSMGQNLYLETAASGTPTT
                     NDPGTNGAGVLNQNYVETSNVNVVEELVSMIQAQRAYELNSKAITVSDQMLQRLGQL"
     gene            668..1327
                     /locus_tag="VKZ48_07865"
     CDS             668..1327
                     /locus_tag="VKZ48_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HLU77802.1"
                     /translation="MKRLAFLSVLLFGGCSIISPHVDVVEPTTARAEPVVQPAVPTGG
                     IYQTASYRPLFEDRRARYVGDILVIQLNEKLNAQKRAASSAEKSGDIGMSVPIIRGLP
                     GKSFQKAEISAQSNNDYEGKGSTESSNLFTGTIAVTVVEVLPNGNLVVSGEKQIGISK
                     NTETLRFSGVVNPASIVPPNSVSSTQVADARIDYRGSGYIDEAQAMGWLSRFFLTFLP
                     F"
     gene            1329..2420
                     /locus_tag="VKZ48_07870"
     CDS             1329..2420
                     /locus_tag="VKZ48_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HLU77803.1"
                     /translation="MRLLLTLLAFLIATPVAAERIRDIASVQGVRANQLIGYGLVVGL
                     DGSGDQTTQTPFTTQTLQSMLSQLGINLPPGMNLQLKNVAAVMVTASLEPFARPGQTI
                     DVTVSSLGNAKSLRGGTLLLTPLKGADGQVYAMAQGNVVVAGAGAAGAGSSVQVNQLN
                     AGRVPAGATVERAVPSPFMTAESIRLELRDTDFDAARRVAEAINSHWGLGTATALDGR
                     VIDVRAPQSPNERVAFLAQLEALPIESIAPAAKVIINARTGSVVMNQAVSVQPCAVAH
                     GSLSIVVSATPVISQPEAFSQGQTVVAQQADIQMRQDGGNLMMVPAGAQLADVVKALN
                     ALGATPIDLLAILQAMKAAGALRAELEII"
     gene            2420..3280
                     /gene="flgJ"
                     /locus_tag="VKZ48_07875"
     CDS             2420..3280
                     /gene="flgJ"
                     /locus_tag="VKZ48_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HLU77804.1"
                     /translation="MAIDPALSSRMALDAGALDDLKTQARQSPDRALRQAAQQFEAVF
                     MNMLLKSMRDTLPQDGPFSSDTTRMYTGMLDQQMAQKFAEGEGLGLADMLVKQLSTHL
                     PQPAPVAAPVATPASAAASSAARMPPAAADTPDAFVERMLPHARIAARRTGLPAQFVI
                     GQAALESGWGKHEIRAADGTPTHNLFGIKAGSHWKGRTVEVRTTEYIDGQPRKVVERF
                     RAYDSYADAFTDWARLMAGNPRYAKVLEAKDAAQFAQRLERAGYATDPLYAEKLTRVI
                     NRQLSARREG"
     gene            3519..5414
                     /gene="flgK"
                     /locus_tag="VKZ48_07880"
     CDS             3519..5414
                     /gene="flgK"
                     /locus_tag="VKZ48_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HLU77805.1"
                     /translation="MASSLFGIGISGLNTAQAGLLTTSHNISNVHTPGFSRQAIVQGS
                     NNPQYTGVGFFGNGAHVQTVKREYNAFLEVQARETQASASHLQRYASQIGRIDQLLGD
                     VESGLSAAFDNFFEGVNAVASYPRDPAARQSMLSGGNALVSRFKVLETQLSDARKDVN
                     SQLQAGVTEINSIASRIAELNDRIAVYSAQGTGSQLPNDLLDQRDTLVRDLNKLIGAN
                     VVEQSDGSYNVFIGNGQALVVRNRANSLSVVDDPQVLGDKQIMLNSGGVQMRLSAAQL
                     TGGELGGLLEFRDQTLNTAHNALGRIALNLAEAFNQQHRLGQDLNDLPGGDYFTVGAP
                     RIHPDDGAVSVALSNVADLTTSDYRLSYDGTDYTLTRLPENVSMTITAEVTSGTEVAG
                     HGITIIIDPLNPMAAGDSYIIQPTRGGAAGLAMAIHDPQAIAAASPIRTAAALDNAGS
                     ATISTGNVLPGYTPSGATHTITFGEVGGVMHYSIDGDTPVQYVSGQAIEFNNIEFTLS
                     GVPAEGDVFTIEPNTGGQTDNRNAILLAALQTRDLMAGGTATLQGGYAQLVADIGNKT
                     RELQVTGDAQAAMLKETMAARESVSGVNLDEEAANLMRYQQAYQASGKVIAIAGSLFD
                     SILDIMR"
     gene            5417..6637
                     /gene="flgL"
                     /locus_tag="VKZ48_07885"
     CDS             5417..6637
                     /gene="flgL"
                     /locus_tag="VKZ48_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HLU77806.1"
                     /translation="MRISTGMIFQRGLDAMQKQQSSLFQTQQHIAANKRVIRPSDDPV
                     AASRAVETTQAKALNKQYADNQAAARDALALGESTLGNIGDVLQDARVLLVNATNGSL
                     TADDRKSLALDLKAKLDQLIGLANTRDGAGGYLFAGYQEQAQPFTPTAAGAQYNGDQG
                     RRELQVAPGRTLAVSENGVELFERLREGNGTFVTASATANTGNAAISVGRATGQVSGA
                     LQYQVEFALDANGAMTYTIFDSGANVIGAPDQPYTSGAAISIDTGTGALEFEISGEPA
                     AGDIFTIEPAGQKNVFAMLSEAIAALERPLPDGAARAAFTEDMARSLQNVDNAFDRVL
                     SVRTAMGASLRELDALNNVVQDNALHYEERLSELIDLDYAAALTQLVREQMALEAAQL
                     SYQRVTSLSLFNYL"
     gene            6670..8517
                     /locus_tag="VKZ48_07890"
     CDS             6670..8517
                     /locus_tag="VKZ48_07890"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HLU77807.1"
                     /translation="MVSSTQQLPSDIAREALRRLAQRRIAPTPDNYAQVYCEISGRDD
                     QSSIMALRLLEGLAADLGKREGALQPGARALALALAERHWEDARKELDELCELATRGP
                     DWGSLLRSLFKQLETRHAAYTDARKREMLDHVLTAFGGDGMKLHARLSGVVKAWSESA
                     PAQRVELTLVSPAKSATEKSAPQPVETPAALERSGETQPLAQSQSQPVSLQTSRELLR
                     KVLWLVADTIERGVVERLTHSPKLAEEARAIIQAAQWASTAAEVDRLGEQLKHFWLKF
                     ELSGEGPDRVIDGLGALLRLVVENVGELVADERWVSAQIDQMREVLDGPIDVRSLREA
                     ERSFRRVLYRQAAAKFSLDEAKNALKSVLTTFIDRIGTMAADTGSYQERMARYAEELA
                     QTDDIHRISDIVQQLTQDTRSMHTDMQRNYDELESARRHAAVHEEKIRVLEQELESIS
                     QQVREDGLTRALNRRGLTEAFVVEAVRSDRDASPLCLAILDVDDFKVLNDRYGHATGD
                     EALVHLSNVIRRTLRPTDIVARYGGEEFVILLPATSIGEAAEIMRRTQRELTRHFFLH
                     DNERLLITFSAGVAQRRGGEEQHALIDRADAALYLAKKRGKNQVAQAEV"
     gene            complement(8550..9182)
                     /locus_tag="VKZ48_07895"
     CDS             complement(8550..9182)
                     /locus_tag="VKZ48_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLU77808.1"
                     /translation="MIRVLVADDHAVVRKGLTQILSDTENIRVVGEAPNAAEAVRLLR
                     QTPCDLLLMDVAMPGKNGIEALKVIKDEFPQVRVLILSMYPEDQFGVRALKAGAAGYL
                     TKDSAPEKLIEAVERIMSGKRYITEGLAELLAESMSPASEVPPHQTLSDREFQTLRLI
                     ASGKKLSEIASTLALSPKTVSVYRTRLLEKMRLTNNAELTHYAIKNGLVD"
     gene            complement(9196..10668)
                     /locus_tag="VKZ48_07900"
     CDS             complement(9196..10668)
                     /locus_tag="VKZ48_07900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLU77809.1"
                     /translation="MRPLRVLVVEDAEADYELVLHALRRARYDVHALRVEDEHAMREA
                     LVHDRWDLVISDHRLPTFSASAALHTLQSSGLDLPFIIVSGAIGEDVAVEAMQSGADD
                     YILKDRLARLIPAVERCLKASEARMRQREAEAALRESESRLRAITANLPGMLFRVHTD
                     EEGIPQAFTYASEAAERLFGVAPQALLDSPHSLHRLIHEADRLRFDTALAHAALARSR
                     LHWEGRAAPRPGHDEEGERWVQIEASPRECDTPGLVWDGLISDISALKQAQADVYASR
                     DELRRLSGHLERAKEQERARIAREIHDDIGGTLTGLRADLAWIKKRFGADPLMAEKLE
                     SMGALLDSAMQASVRIARDLRPPILDFGFGAAIEWQAKDFQKRTGVACRFRCEGDAEP
                     DTDRATAVFRIFQELLTNIAKHAQATQVEVTMQSDGRMLRLEVLDDGIGLAESDRSKR
                     GSYGIRGMIERACELDGRLVFHRPPRGGTRALLELPLSAP"
     gene            11009..>11583
                     /locus_tag="VKZ48_07905"
     CDS             11009..>11583
                     /locus_tag="VKZ48_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77810.1"
                     /translation="MTGGSLSVSLAMLGAVALGWAVLLHTCRQRMRAECEQRGEEVRR
                     RVAEVAAQTERALAQCTEIYGEQCAAVTNELTQLEGILADAIAKLVESFNALHAHSVN
                     QQNVCVAITRGEAIDGCSAAAVSFDHFIQETSTTLRAFVDEMTQNGQTATVLVDRVNG
                     IREQVDRILSVLGEIEGISGQTNLLALNAAI"
BASE COUNT         2156 a         3771 c         3773 g         1883 t
ORIGIN      
        1 ctgctctatc agaccgtgcg ccagccgggt gcgcagtcct ctcagcagac cacggtgccg
       61 gccggcctgc agatcggcac gggcgcgaag tcggtggcca acgtgcgcat ccatacgcag
      121 ggcaatctcc agctcaccgg caatgcgctg gacgtggcga tcaacggtca gggttttctg
      181 cagatcctcc tgcccgacgg caccaccgcc tacacgcgcg acggcagctt ccatgtcgac
      241 gccaacggac aggtggtgaa cgccaacggc tatccgctgc agccggcggt caccatcccg
      301 cccaatgcca gcaacatcac catcggctcc gacggcacgg tgacggtgat gcagcccggg
      361 caggtcaacc ccgtgcaagt gggccagatc cagttggcga ctttcgtcaa tccggccggc
      421 ctgcattcca tgggacagaa cctgtacctg gagaccgccg cctcgggcac gccgaccacc
      481 aacgatccgg gcaccaacgg cgcgggcgtg ctgaatcaga attacgtcga gacctccaac
      541 gtcaacgtgg tggaggaact cgtcagcatg atccaggcgc agcgcgcgta cgaactcaac
      601 tccaaggcca tcacggtctc cgaccagatg ctgcagcgcc tgggacaact ctaaatccgc
      661 gcgcacgatg aaacggctcg ctttcctgtc ggttctgctg ttcggcggct gcagcatcat
      721 cagcccgcat gtggacgtgg tcgaaccgac cacggcgcgc gcggagcccg tcgtgcagcc
      781 ggccgtaccg accggcggca tctatcagac ggcctcgtac cggccgctgt tcgaggaccg
      841 tcgcgcgcgt tacgtcggcg acatcctcgt catccagttg aacgagaaac tcaacgccca
      901 gaagcgtgcg gcgtcctccg cggagaaatc gggcgacatc ggcatgagcg tgcccatcat
      961 ccgcggtctg ccgggcaaga gcttccagaa agccgagatc agcgcgcaga gcaacaacga
     1021 ctacgagggc aaggggtcga ccgaatcgtc gaacctgttc accggcacga tcgccgtcac
     1081 cgtggtggaa gtgctgccca acggcaatct cgtggtatcg ggcgagaagc agatcggcat
     1141 cagcaagaac accgaaacgc tgcgcttctc cggtgtggtg aatcccgcca gcatcgtgcc
     1201 gccgaactcc gtgagctcca cccaggtggc cgatgcacgc atcgactacc gcggctcggg
     1261 ctatatcgac gaagcgcagg cgatgggctg gctgtcgcgc ttcttcctca ctttcctgcc
     1321 gttctgatat gcgactgctg ctcaccctac tcgccttcct gatcgcgacg ccggttgcgg
     1381 ccgagcgtat tcgcgacatc gcctcggtgc agggcgtgcg cgccaaccag ttgatcggct
     1441 acggcctcgt cgtcggcctc gacggcagcg gcgaccagac cacgcagacg ccgtttacca
     1501 cgcagaccct gcagtcgatg ctgagccagc tcggcatcaa tttgccgccg ggcatgaacc
     1561 tgcaattgaa gaacgtcgca gcggtgatgg tgaccgcctc gctagagcct ttcgcgcgac
     1621 cgggccagac catcgatgtc acggtgtcct cgctcggcaa cgccaagagc ctgcgcggcg
     1681 gtacgctgct gcttacgccg ctcaagggtg cggacggcca ggtttacgcc atggcgcagg
     1741 ggaacgtcgt cgtcgccggc gccggtgccg cgggcgcggg cagttcggta caggtcaacc
     1801 aactcaacgc gggccgcgtg cccgcgggtg cgacggtgga acgcgccgtg ccctcgccgt
     1861 tcatgacggc ggagagcatc cggctcgaat tgcgcgatac cgatttcgac gccgcgcggc
     1921 gcgtggccga ggcgatcaac agccactggg gcctgggcac ggcgacggcg ctcgacggcc
     1981 gcgtgatcga cgtgcgcgcg ccgcagagtc ccaacgaacg cgttgccttc ctcgcgcaac
     2041 tggaagcact gccgatcgaa tcgatcgcgc cggcggccaa ggtgatcatc aatgcgcgca
     2101 ccggctctgt ggtgatgaac caggcggtga gcgtgcagcc gtgcgcggtg gcgcacggca
     2161 gtctgtccat cgtggtgagc gcgacgccgg tgatcagcca gcccgaagct ttctcgcagg
     2221 ggcagacggt ggtggcgcag caggccgaca tccagatgcg tcaggacggc ggcaatctga
     2281 tgatggtgcc cgccggcgcg caactggccg acgtggtgaa ggcgctgaac gcgctgggcg
     2341 ccacaccgat cgatctgctc gccatcctgc aggcgatgaa agcggccggc gcgctgcgcg
     2401 cggagttgga gatcatctga tggccatcga ccctgcgctc tcctcgcgca tggcgctcga
     2461 cgccggcgcg ctggacgatc tcaagacgca ggcgcggcag tcgcccgatc gcgcgctgcg
     2521 tcaggcggcg cagcagttcg aggcggtgtt catgaacatg ctgctgaaga gcatgcgcga
     2581 cacgctgccg caggacggcc cgttctcctc cgacacgacg cgcatgtaca ccggcatgct
     2641 cgatcagcag atggcgcaga aattcgccga gggcgaaggt ctcggactgg ccgacatgct
     2701 ggtgaagcag ttgtcgacgc atctgccgca gccggcgccc gttgcggcac cggtcgcgac
     2761 gcctgcatcg gctgccgcgt cgtcggcggc gcgcatgccg ccggccgcag ccgatacccc
     2821 cgacgccttc gtcgagcgca tgctgccgca tgcacgcatc gccgcgcgcc gcaccggcct
     2881 gcccgcgcag ttcgtcatcg gtcaggcggc gctggagtcg ggctggggca aacacgaaat
     2941 cagggcggcg gacggtacgc cgacgcacaa cctgttcggc atcaaggcgg gcagccactg
     3001 gaaggggcgc acggtcgagg tgcggacgac ggaatacatc gacggccagc cgcgcaaagt
     3061 cgtcgagcgc ttccgcgctt acgactcgta tgccgacgct ttcaccgact gggcgaggct
     3121 gatggccggc aacccgcgct acgcgaaggt gctggaggcg aaggatgcgg cgcagttcgc
     3181 ccagaggctc gaacgcgccg gatacgcgac cgatccgttg tatgcggaaa aactcacgcg
     3241 ggtgatcaac cggcagctct cggcgcgacg cgaaggctga gatgagagga aaggcggaac
     3301 cacagaggca cagagtgaaa agcttcaaac gcttcaccac caagacacca agacaccaag
     3361 aaaaccttgt ccaagcgacc ttggtgcctt ggtgcgttgg tggttcgaga tctttgtctt
     3421 tctctgtgtc tccgtgcctc tgtggttcag gtttttaaat ggcgcctcaa gttcggcgtc
     3481 gcggcgccga tatttgaatg aacatgcgag tgcgataaat ggccagttcc cttttcggta
     3541 tcggtatcag cggcctgaac accgcacagg cgggattgct gacgaccagt cacaacatca
     3601 gcaatgtgca tacgcccggc ttcagccgac aggcgatcgt tcagggctcc aacaatccgc
     3661 aatacaccgg cgtcggcttt ttcggcaacg gcgcgcatgt gcagaccgtc aagcgcgagt
     3721 acaacgcctt cctcgaagtg caggcgcgcg aaacgcaggc ctcggccagc cacctgcagc
     3781 gctacgccag ccagatcggc cgcatcgacc aactgctcgg cgacgtggaa tccggcctct
     3841 cggccgcctt cgacaatttc ttcgagggcg tgaacgccgt cgcctcctat ccgcgcgatc
     3901 cggccgcgcg gcagagcatg ctgtcgggcg gcaacgcgct ggtgagtcgc ttcaaggtgc
     3961 tggaaacgca attgagcgac gcgcgcaagg acgtgaacag ccagctccag gccggcgtca
     4021 ccgaaatcaa ctccatcgcc tcgcgcatcg ccgaactgaa cgaccgcatt gcggtgtaca
     4081 gcgcgcaggg caccggcagc cagttgccga acgatctgct cgaccagcgc gacacgctgg
     4141 tgcgcgacct caacaagctg atcggcgcga atgtggtgga gcagagcgac ggcagctaca
     4201 acgtctttat cggcaacggc caggcgctgg tggtgcgcaa tcgcgccaac agcctgtcgg
     4261 tcgtggacga tccgcaagtc ctcggcgaca agcagatcat gctcaacagc ggcggcgttc
     4321 agatgcgcct gagcgcggcg cagctcaccg gtggcgaact gggcgggttg ttggagtttc
     4381 gcgatcagac gctgaacacg gcgcacaacg cgctgggccg tatcgccctg aatcttgcgg
     4441 aggccttcaa tcagcagcac cgtctgggcc aggacctcaa cgacctgccg ggcggcgact
     4501 acttcaccgt gggtgcgccg cggatccatc ccgacgatgg cgccgtttcg gtcgctctct
     4561 cgaacgtcgc cgacctgacg accagcgact atcgcctgag ctacgacggc acggactaca
     4621 cgctcacgcg gctgccggag aacgtctcga tgacgattac ggcggaagtg acgtcgggca
     4681 cggaggtggc cggtcacggc atcaccatca tcatcgatcc gctgaatccg atggcggcgg
     4741 gcgacagcta catcatccag cccactcgcg gcggcgctgc gggccttgcg atggcgatac
     4801 acgatccgca ggctatcgcg gccgcctcgc ccatccgcac ggcagcggcg ctcgacaatg
     4861 ccggcagcgc gaccatcagc accggcaacg tgctgcccgg ctataccccg agcggcgcga
     4921 cccacacgat cactttcggt gaggtgggcg gtgtcatgca ctacagcatc gacggtgata
     4981 cgccggtgca gtacgtcagc ggccaggcaa tcgaattcaa caacatcgag ttcacgctga
     5041 gcggcgtacc cgccgaaggc gacgtcttca ccatcgagcc gaacacgggc gggcagaccg
     5101 acaaccgcaa tgcgatcctg ctcgcggccc tgcagacgcg ggacctcatg gcgggcggca
     5161 ccgccacgct gcagggcggc tacgcgcaac tggtggcgga tatcggcaac aagacgcgag
     5221 aactgcaggt gacgggcgat gcgcaagcgg ccatgctgaa agagacgatg gcagcgcgcg
     5281 aatcagtgtc cggcgtcaat ctggacgagg aggcggccaa tctgatgcgc tatcagcagg
     5341 cctaccaggc ctcgggcaag gtcatcgcca tcgccggttc gctgttcgac agcattctgg
     5401 acatcatgag gtagtcatgc ggatcagcac gggaatgatt ttccagcgcg ggctggacgc
     5461 catgcagaag cagcagtcca gtctgttcca gacgcagcag cacatcgccg cgaacaagcg
     5521 cgtcatccgt ccgtcggacg atcccgtagc cgccagccgc gcggtggaga ccacacaggc
     5581 gaaggcactc aacaagcagt acgccgacaa ccaggcggcg gcgcgagatg cgttggccct
     5641 tggcgaatcc acgctgggca acatcggcga cgtgctgcag gatgcccgcg tgctgctggt
     5701 caatgcgacc aacggctcgc tgaccgcaga cgatcgcaag agtctggcgc tcgatctcaa
     5761 ggccaagctc gatcaactga tcggtctggc caatacgcgc gatggcgcgg gcggttatct
     5821 cttcgccggt tatcaggagc aggcgcaacc cttcaccccg accgccgccg gcgcgcaata
     5881 caacggcgac cagggccggc gtgaactgca ggtggcgccc ggccgcaccc tggccgtgag
     5941 cgaaaacggc gtggagttgt tcgagcggct gcgcgaaggc aacggcactt tcgtcaccgc
     6001 atcagcaacg gcgaacaccg gcaacgcagc catcagcgtc ggccgcgcca ccgggcaggt
     6061 ttcgggtgcg ttgcaatatc aggtggagtt tgcactggat gcgaacggtg ccatgactta
     6121 caccatcttc gattcaggcg cgaatgtcat cggcgcgccg gatcagccct acaccagcgg
     6181 cgcagcaatc agtatcgaca ccggcacggg tgccctggaa ttcgagatct ccggcgagcc
     6241 cgcggccggc gacatcttca ccatcgagcc tgcagggcag aagaacgtgt tcgccatgct
     6301 gagcgaagcg atcgccgcgc tcgagcggcc gctacccgac ggcgcggcgc gcgcggcttt
     6361 taccgaggat atggcgcgct cgctgcagaa cgtcgacaac gccttcgatc gcgtgctgtc
     6421 cgtacgcacg gcgatgggcg cgagcctgcg cgaactcgac gcactgaaca acgtggtgca
     6481 ggacaatgcg ctgcactacg aggagcggct gtcggaattg atcgacctcg attacgccgc
     6541 cgcactgacg caactcgtgc gcgaacagat ggcgctggaa gccgcgcaac tgagctacca
     6601 gcgcgtgacc tctctgtcgc tcttcaacta cctatgacgg gttgaagtca ttcgccgcat
     6661 cgcaccgcca tggtgagcag tacacagcaa ctaccttcgg acatcgcgcg tgaggcgctg
     6721 cgccggctgg cgcagcggcg catcgcgccc acgccggaca actacgccca ggtctattgc
     6781 gaaatctccg gccgcgacga tcaatcctcg atcatggcgc tgcgcctgct cgaaggcctg
     6841 gccgccgatt tgggcaagcg cgaaggcgcg ctgcaacccg gcgcgcgcgc cctggcgctg
     6901 gcgctggccg agcgccactg ggaagacgcc cgcaaggaac tggacgagtt gtgcgaactg
     6961 gcgacgcggg gcccggactg gggatcgttg ctgcggtcgc tgttcaaaca gctggaaacg
     7021 cgtcacgccg cctacaccga tgcgcgcaag cgcgagatgc tcgatcatgt gctgaccgcc
     7081 ttcggcggcg acggcatgaa gctgcatgcg cgactgagcg gggtggtcaa ggcctggtcc
     7141 gaaagcgcgc cggcgcagcg cgtcgaactg acgctggtgt cgccggcaaa atccgcgacg
     7201 gagaaaagcg ccccgcagcc ggtcgaaacg cctgccgcac tcgaacgcag cggcgaaacg
     7261 cagccgcttg cgcaaagtca gagccaaccc gtttccctgc agaccagccg cgaactgttg
     7321 cgcaaggtac tgtggctcgt cgccgatacc atcgaacgcg gcgtggtgga gcgactgacg
     7381 cattcgccca aactcgccga ggaggcgcgc gccatcatcc aggccgcgca gtgggccagc
     7441 accgccgccg aggtcgatcg gctgggcgag caactgaagc atttctggct gaagttcgag
     7501 ttgtccggcg aagggccgga ccgggtcatc gacgggttgg gcgcgctgct gcgactggtc
     7561 gtggaaaacg tcggcgaact ggtggccgac gaacgctggg tgtccgcgca aatcgatcag
     7621 atgcgcgaag tgctggacgg cccgatcgac gtgcgcagcc tgcgggaagc cgagcgcagc
     7681 ttccgtcgcg tgctgtatcg gcaggccgcc gccaagtttt cgctcgacga ggcgaagaat
     7741 gcgctgaaga gcgtgctcac cacgttcatc gaccgcatcg gcaccatggc ggcggacacc
     7801 ggcagctatc aggagcggat ggcgcgctac gccgaggaac tggcgcaaac cgacgatatc
     7861 catcgcatca gcgacatcgt gcagcagctc acgcaggata cgcgcagcat gcacactgat
     7921 atgcagcgca actacgacga attggaatcc gcccgccggc acgcggccgt acacgaggaa
     7981 aagatccgcg tactggagca ggaactggag tcgatcagcc agcaggtgcg cgaagacggg
     8041 ctgacgcgcg cgctgaaccg gcgcggcctg accgaagcct tcgtggtcga ggcggtgcgc
     8101 agcgatcgcg acgcgtcgcc tttgtgtctg gccatcctgg atgtcgacga tttcaaggtg
     8161 ctcaacgacc ggtacggtca tgccacaggt gacgaggcgc tggtgcacct gtccaatgtg
     8221 atccgcagga cgctgcggcc gacggatatc gtcgcgcgct acggcgggga ggagttcgtc
     8281 atcctgctgc cggcgacgag tatcggcgag gcggcggaaa tcatgcgccg cacgcagcgc
     8341 gaacttacgc gacacttctt cctgcacgac aatgaacggc tgctcatcac gttcagcgcc
     8401 ggtgtggcgc agcgtcgcgg aggcgaggag cagcacgcgc tgatcgatcg tgccgacgcg
     8461 gccttatacc tggccaagaa gcggggcaag aatcaggtgg cgcaggcgga agtttgagag
     8521 tgcagtcgac tctcgactaa aaccaagact caatccacca gcccgttctt gatcgcatag
     8581 tgcgtcagtt cggcgttgtt ggtgaggcgc atcttttcca gcagccgcgt gcgatagacg
     8641 ctcacggtct tgggcgagag tgcgagcgta ctggcgattt ccgacagttt cttgcccgag
     8701 gcgatgagac ggagggtctg gaactcgcga tcggagagcg tctggtgcgg tgggacctcg
     8761 ctggccggac tcatgctttc ggccagcagt tcggcaagtc cttcggtgat gtaacgcttg
     8821 ccggacatga tgcgctcgac cgcttcgatc agtttctcgg gtgcgctgtc cttggtgaga
     8881 tagcccgcgg cgcccgcctt gagcgcgcgt acgccgaact ggtcttccgg gtacatggac
     8941 agaatgagca cccgcacttg cggaaactcg tccttgatga ccttgagcgc ctcgatgccg
     9001 ttcttgcccg gcatggcgac atccatgagc agcaggtcac agggtgtctg gcgtagcagg
     9061 cgtaccgcct cggccgcatt gggcgcttcg cccacgacac ggatgttctc ggtgtcggaa
     9121 agaatctggg tcagcccctt gcgcacgacc gcgtgatcgt ccgcaaccag cactcgaatc
     9181 aagacaaact cctccctatg gcgccgacag cggcagttcc agcagcgcac gtgtgccgcc
     9241 gcgcggcggc cggtggaaca ccagccgacc atccagttcg cacgcgcgtt cgatcatgcc
     9301 acggatgccg tacgagccgc gcttgctgcg gtcgctttcg gccaggccga tgccgtcgtc
     9361 cagcacttcc agccgcagca tgcggccgtc gctttgcatg gtgacttcga cctgcgtggc
     9421 ctgcgcgtgc ttggcgatat tggtcagcag ctcctgaaaa atccggaaca ctgccgtggc
     9481 gcggtccgtg tccggttccg catcgccttc gcaacggaag cggcaggcga cgccggtccg
     9541 cttctgaaaa tccttcgcct gccattctat ggcggcgccg aagccgaaat ccaggatggg
     9601 cgggcgcaga tcgcgggcaa tccgcacact tgcctgcatt gcgctgtcga gcagcgcacc
     9661 catgctctcc agtttctcgg ccatgagcgg gtcggcgccg aagcgctttt tgatccaggc
     9721 caaatccgcg cgcagaccgg tgagcgtgcc gccgatgtcg tcgtggattt cccgcgcgat
     9781 gcgtgcgcgc tcctgttcct tggcgcgttc cagatggccc gacagccggc gcagttcgtc
     9841 gcgcgaagcg tagacgtccg cctgggcctg cttgagcgcg ctgatgtcgc tgatcaggcc
     9901 gtcccacacc agaccgggcg tgtcgcattc gcgggggctt gcttcgatct gcacccagcg
     9961 ttcgccttcc tcgtcatggc cgggacgcgg cgcggcgcgt ccttcccagt gcagccgtga
    10021 gcgcgcgagc gcggcgtgcg ccagcgcagt atcgaagcgc agccggtccg cctcgtggat
    10081 cagccggtgc aggctgtgcg gactgtcgag cagcgcctgc ggcgcgacgc cgaacaggcg
    10141 ctcggccgct tcgctggcat aagtgaaggc ctgcgggatg ccttcctcat cggtatgcac
    10201 gcgaaacagc atgccgggca gattggcggt gatggcgcgc agccgcgatt cggattcgcg
    10261 caacgcggct tcggcttcgc gctggcgcat gcgcgcctcg ctggccttca gacaccgttc
    10321 gaccgcgggg atgagccgcg ccagacggtc cttgagaatg tagtcgtcgg cgcccgattg
    10381 catcgcctcc accgccacgt cttcgccgat ggcgccggaa acgatgatga agggcagatc
    10441 gagacccgag gattgcagcg tgtgcaaggc ggcgctggcg gagaaggtcg gcaggcggtg
    10501 gtcggaaatc accagatccc agcgatcgtg cacaagcgcc tcgcgcatcg cgtgctcgtc
    10561 ctccacgcgc agcgcatgca cgtcgtatcg ggcgcgccgc agcgcgtgca acacgagttc
    10621 gtaatcggcc tcggcgtcct ccaccacgag cacgcgcagc ggacgcaatg gagcactgcg
    10681 gtcgaggttg atgccctcgg acgaggtggc cggatggctt tgattcgttg tttcgatgac
    10741 ctacccctcg cacttcgcga ccggcgccaa cgtgccgcgc ggcgcatttg ccgcctcaaa
    10801 ccgtgaacag cagcacgatt tgcaggggct tccacccttt atttgtgcga tttgccgccg
    10861 ataccagact caataatcga cgtcaggcca tgtggcctgc gtgcacgtcc gtgcacaagt
    10921 caaatttgag cggaccaagt ctagcatgag caacaagctg ttccttcgag gctttggact
    10981 gagtgccgcg gccgcggtgc cggttttgat gactggcgga tcattgtccg tttcgctggc
    11041 aatgctgggt gccgtcgcgc tgggctgggc ggtactttta cacacctgcc ggcaacgtat
    11101 gcgcgccgaa tgcgaacagc gcggcgagga ggtgcgtcgt cgcgtggccg aggttgccgc
    11161 gcagaccgag cgcgcgctgg cgcaatgcac cgaaatctac ggcgaacagt gcgcggccgt
    11221 cacgaacgag ctgacccagt tggaaggcat cctggccgac gctatcgcca agctggtgga
    11281 aagcttcaac gcgctgcatg cgcacagtgt gaaccagcag aacgtctgcg tcgccatcac
    11341 ccgcggcgaa gcgatcgacg gctgcagcgc ggccgcggtc tccttcgacc acttcattca
    11401 ggagacgtcg accacgctgc gtgccttcgt cgatgaaatg acccagaacg gccagacggc
    11461 gaccgtgctg gtcgaccgcg tcaacggcat ccgggagcaa gtcgatcgca tcctgtcggt
    11521 gctgggcgag atcgaaggca tttcaggcca gaccaatctg ctcgcgctca atgccgcgat
    11581 cga
//
LOCUS       DATGOU010000137         3892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_4865926, whole genome shotgun sequence.
ACCESSION   DATGOU010000137 DATGOU010000000
VERSION     DATGOU010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4865926"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            399..833
                     /locus_tag="VKZ48_07910"
     CDS             399..833
                     /locus_tag="VKZ48_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLU77811.1"
                     /translation="MLTKEILAIKGNALYTIPPQGLLREAVSCMVDYDIGSLVVMDHG
                     KLAGMLTFREVLAALDEGKGSLGDRRVSEVMVREPHVVGPDMQVDDLRRLMLETRARY
                     MPVLDKDVLIGVLSFHDVAKAVLEEQSFENDMLKGYIKNWPA"
     gene            924..2027
                     /gene="aroC"
                     /locus_tag="VKZ48_07915"
     CDS             924..2027
                     /gene="aroC"
                     /locus_tag="VKZ48_07915"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HLU77812.1"
                     /translation="MSGNTLGRLYCVTSFGESHGPAYGGVVDGCPPGLELSAADLQAE
                     LDRRKPGTSRHVTQRRESDTVEILSGVFEGRTTGTPIGFIIRNEDQRSKDYGNIAETF
                     RPGHADYTYWQKYGTRDYRGGGRASARETVVRVAAGAIAKKWLRERYGVTVRGYLSQL
                     GPHELPFVSWEAVSQNPFFAADPGAVAKLESYMDELQRAGDSVGAKLTVVAENVPAGW
                     GEPVYDRLDAEIAYAMMGINAAKGVEIGAGFASVAQRGSEHGDELTPEGFASNHAGGM
                     LGGISTGQDIVVHVALKPTSSIRLPRRSIDKQGQPTVVQTTGRHDPCVGIRATPIAEA
                     MLAIVLMDHALRHRAQNADVTTDTPKIPGHIRS"
     gene            2066..3226
                     /locus_tag="VKZ48_07920"
     CDS             2066..3226
                     /locus_tag="VKZ48_07920"
                     /inference="COORDINATES: protein motif:HMM:NF037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLU77813.1"
                     /translation="MHVPAFRLSALYFAYFAFIGAYMPYFALYLASLGLAPWAIGVLL
                     SVGQFTRIFAPNIWAWFADRRGRSIELLRWALMLAVASYFGLFYADSFGALFVVLAVH
                     AFFVSASMPLFESMTLSYLHGAYGRYGPIRLWGSVGFIVAVTLIGAALDYVATRNFLW
                     MALLPLAGALIASWAIAQPPSRPSAAGEGEISVRRLALRPEVLALFGAGFLMCAAHGP
                     LYTFYSIYLDLEGYSKTTVGLLWALGVVAEILVFLWMPKWLSRYSPYKVLLVSFALAA
                     LRFVMIGWGVNSLVVLIAAQIMHAASFGSFHAAALTLVNAWFVGRGHVRGQALYSSLT
                     YGAGSAAGAAGAGALWQSVGPAWTFTIAGMAAACGLLLIAWRGRLFPQLSHA"
     gene            3219..>3892
                     /locus_tag="VKZ48_07925"
     CDS             3219..>3892
                     /locus_tag="VKZ48_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017452968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HLU77814.1"
                     /translation="MPDSAMKRFVCALALSLLVSACATTSTTSSGAVGIDRGQTMLIS
                     SEEIDRASGQQYAQMMAEARKKGVLNRDAAQVKRVQTIVQRLIPTTAVFRPDAVNWKW
                     EANVITSKELNAWAMPGGKIAVYTGLIEQLKLTDAELAAVLGHEIAHALREHSRERIS
                     RQMATGAVVGIGAAILGVGQGGADLASMVADVTLNRPNSRLHESEADAIGTELAARAG
                     YDPRAAV"
BASE COUNT          624 a         1214 c         1283 g          771 t
ORIGIN      
        1 aggctcgaag gtgccgtcgc ttacgtgatg aatctgttcg ctcatgtagg tctattcctt
       61 aacgggtcat tccggaagcg gcgggtcgcc gcgtagggga ggcgtggatc ggtacgggag
      121 taatgctagc aaaaaacttg cagcgggtga ttcggggccg gccggttgcg gcaggttgac
      181 gcgacactcg atccgcaagt gacggaatgc cgtctcatac acgaccgacg cgcgaccgtg
      241 ccggcgggcg cgagatttgc ctagcccctt gtgcggtgca tcattgcggg taagatcgca
      301 aacaggacga agcgcggcct gccgcgtttc cgaacggcca acgtaatcgc gtccggcgtt
      361 cgccgttgtc ggtcccaacg gtcgcgggtg gaggcatcat gctgaccaaa gaaatcctcg
      421 ccatcaaggg caacgccctc tacaccatcc ctccccaggg ccttttgaga gaagcagtca
      481 gttgcatggt ggattacgac atcggttcgt tggtcgtgat ggaccacggc aagcttgccg
      541 gcatgctgac ctttcgcgag gtgcttgccg cgctggatga aggcaagggg tcgctcggcg
      601 atcgtcgcgt cagcgaagtg atggtgcgcg agcctcatgt cgtgggtccc gacatgcaag
      661 tcgacgatct gcgccggttg atgctcgaaa cccgcgcgcg ctacatgccg gtgctcgaca
      721 aggacgtgct gatcggcgtg ctgtccttcc atgacgtcgc caaggcggtc ctcgaagagc
      781 agagtttcga aaacgacatg ctcaagggct atatcaagaa ctggccggca tgaggcctcg
      841 gaccgtgccg cggacgtgcg ccgcggcgcg gcagccggag gggcgcatgc taaactcgct
      901 cgcctctcta aatccacgca cgcatgtccg gtaatactct aggcagactc tactgcgtta
      961 cttccttcgg cgagagccac ggccccgctt atgggggcgt agtggacggc tgtccgcccg
     1021 gtctggaact gtccgcggcc gacctgcagg cggaactcga ccggcgcaag cccggtacct
     1081 cgcgccacgt cacgcagcgt cgcgagtcgg atactgtgga aatcctgtcc ggcgtctttg
     1141 aaggacgcac gaccggcacg cctatcggct tcatcatccg caacgaggat cagcgctcga
     1201 aggactacgg caacatcgcc gagaccttcc gccccggcca tgccgattac acctactggc
     1261 agaaatacgg tacgcgcgac tatcgcggcg gcggacgcgc ctccgcgcgc gaaacggtcg
     1321 ttcgcgtggc ggcgggcgcc attgcgaaga agtggctgcg tgaacgttat ggcgtgacgg
     1381 tgcggggtta tttgtcccaa ctcggtccgc acgaactgcc cttcgtgtcc tgggaagccg
     1441 tgtcgcagaa tccgtttttt gccgcggacc ccggggcggt cgccaagctc gagtcgtata
     1501 tggacgagtt gcagcgcgcg ggcgattcgg tcggcgccaa gctcaccgtc gtggccgaga
     1561 atgttcccgc cggctggggc gaaccggttt acgaccggct cgatgcggag attgcgtacg
     1621 cgatgatggg catcaacgcc gcaaaggggg tggagatcgg tgcgggtttc gcatcggttg
     1681 cccagcgcgg cagcgagcac ggcgacgaac tgacgcccga aggcttcgcc tccaatcatg
     1741 ccgggggcat gctgggcggc atttccaccg ggcaggacat cgtcgtgcac gtggcgttga
     1801 aaccgacttc cagcatccgt ctgccgcgcc gctccatcga caagcagggt caaccgaccg
     1861 tggtccagac gaccggccgg cacgatccct gtgtgggcat ccgcgccacg cctatcgccg
     1921 aggccatgct ggcgatcgtg ttgatggatc acgcgctgcg tcaccgtgcg cagaatgccg
     1981 acgtgacgac cgacacgccc aagattccgg gacacatccg ctcctgaacc cgctggcgat
     2041 acacgccggc ctcgcctgac gcgtcgtgca cgttcccgct ttccgacttt cggcgcttta
     2101 tttcgcgtac ttcgcgttca tcggcgccta catgccgtat ttcgcgctgt acctcgcctc
     2161 gctggggctc gcgccctggg cgatcggcgt cctgctctcg gtcgggcagt tcacgcgcat
     2221 cttcgcgccg aatatctggg cctggttcgc cgaccggcgc ggccgctcca tcgaactgtt
     2281 gcgctgggcg ctgatgctcg ctgtggcgag ttacttcggc ctcttctatg ccgacagttt
     2341 cggcgcgctg ttcgtcgtgc tcgccgtgca tgcctttttc gtcagcgcat ccatgccgct
     2401 gttcgagtcg atgaccttga gctatctgca cggcgcttac ggccgctatg gaccaattcg
     2461 gctgtggggt tcggtgggct tcatcgtggc ggtcacgctg atcggggcgg cgctcgatta
     2521 cgtcgccacg cgcaatttcc tctggatggc gctgctgccg ctggccggtg cgttgattgc
     2581 ttcctgggcc atcgcgcagc cgccgtcgcg tccgtcggca gcgggagagg gtgagatttc
     2641 cgtgcgcagg ctggcgctgc ggccggaagt gttggcgctg ttcggcgcgg gctttttgat
     2701 gtgcgccgcc catggccccc tctacacctt ctactcgatc tatctggatc tggagggcta
     2761 cagcaagacc acggtcggcc tgctgtgggc gctcggcgtg gtggccgaga tcctggtatt
     2821 cctgtggatg cccaaatggt tgtcgcgtta ttcgccttac aaggtcttgc tcgtaagctt
     2881 cgcgctcgcc gcgttgcgct tcgtcatgat tggctggggc gtgaacagct tggtggtgct
     2941 gatcgccgcc caaatcatgc acgcggcgag ctttggcagt tttcatgcgg ctgcgttaac
     3001 cttggtcaac gcttggttcg tcggccgcgg ccatgtgcgc ggacaggcac tctattccag
     3061 tctcacctac ggggcgggaa gtgcagccgg cgcggccggc gccggtgcgc tgtggcaaag
     3121 cgtcgggccg gcgtggacct tcaccatcgc cggcatggcg gcggcctgcg gcttgctgct
     3181 gatcgcttgg cgcggccgac tgttccctca actttcgcat gcctgattcc gctatgaaac
     3241 gcttcgtctg tgcgcttgcc ttgagcctcc tggtgagcgc ctgcgctacc accagcacca
     3301 cttcgtccgg cgcggtcggt atcgaccgtg ggcagaccat gctgatttcc tccgaggaaa
     3361 tcgatcgcgc gtccggtcag cagtacgctc agatgatggc ggaggcgcgc aagaagggtg
     3421 tgctcaaccg cgatgcggcg caggtgaaac gggtgcagac catcgtccag cgcttgattc
     3481 cgaccacggc cgtcttccgg cccgacgcgg tgaattggaa gtgggaggcc aatgtcatca
     3541 cttccaagga actcaatgcg tgggcgatgc ccggcggcaa gatcgcggtg tacaccgggc
     3601 tgatcgaaca actgaaactg accgatgcgg aactggccgc cgtgctgggt catgaaatcg
     3661 cccatgcgtt gcgcgagcat tcgcgcgaac gtatttcccg ccagatggcc accggggcgg
     3721 tggtcggtat cggcgcggcc atactgggcg tcggacaggg cggcgccgat ctggcgagca
     3781 tggtggcgga cgtgaccctc aaccggccga attcccgtct gcatgaaagc gaggcggacg
     3841 ctatcggtac cgaactggcc gcgcgcgccg gctacgatcc gcgcgcggcc gt
//
LOCUS       DATGOU010000138        17415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_2655799, whole genome shotgun sequence.
ACCESSION   DATGOU010000138 DATGOU010000000
VERSION     DATGOU010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 17415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17415
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2655799"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1098
                     /locus_tag="VKZ48_07930"
                     /pseudo
     CDS             1..1098
                     /locus_tag="VKZ48_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612104.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1224..2222
                     /locus_tag="VKZ48_07935"
     CDS             1224..2222
                     /locus_tag="VKZ48_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HLU77815.1"
                     /translation="MTKTAPPAAADTPAALRLARDVLQIEAAAITALVERLDDTFVRA
                     VQLILDCDSRVVVSGIGKSGHVGRKIASTLASTGTPAFFVHPAEASHGDLGMITRRDV
                     MIAISYSGESDELLTIIPLVKRQGAKLIAMTGNPQSTLAAEADVHLNVHVAKEACPLN
                     LAPTASTTATLALGDALAVALLDARGFKADDFARSHPGGSLGRRLLTHVNDVMKRGKD
                     VPAVRLGAPLTDAILEISRKGMGMTAIVDEHDRVLGIFTDGDLRRAVERGVDLRGAAI
                     SEVMTPSPLTIAATELAVEAVALMENRRINQLLVTDAEQKLIGALNMHDLFQAKVV"
     gene            2225..2755
                     /locus_tag="VKZ48_07940"
     CDS             2225..2755
                     /locus_tag="VKZ48_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010394575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HLU77816.1"
                     /translation="MDRTEALARARNIRLMSFDVDGVLTDGTLYLSDAGEEIKGFNTL
                     DGLGLKLLQDNGVELAIITGRESKLVALRAQNLGIRRLHQGVERKLEIFDALRAELGL
                     SLEQCGYMGDDLPDLPILTRCGFAVTVPQAPAAVRARAHYVTEARAGAGAAREVCEFI
                     LQAQGRLEQAIAPYLA"
     gene            2752..3339
                     /gene="lptC"
                     /locus_tag="VKZ48_07945"
     CDS             2752..3339
                     /gene="lptC"
                     /locus_tag="VKZ48_07945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HLU77817.1"
                     /translation="MKPRSSTLFPLVLMIGLALVSLWLEHAVRGGDQSAHSALRHDPD
                     FIIENFTTTDIGGQGRARATLRAKRMVHYPDNDTSELEEPYLVHLNPDRPPLHISAER
                     GLVAGTGEEVELFDNVVVRRLAAGQQAELRIQTSYLQVFPDKHLARTPAEVLITEGAS
                     RLKGVGMEVNSSTGHLKLQSKVSGFYVKSGASSGK"
     gene            3378..3968
                     /gene="lptA"
                     /locus_tag="VKZ48_07950"
     CDS             3378..3968
                     /gene="lptA"
                     /locus_tag="VKZ48_07950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HLU77818.1"
                     /translation="MPVYFPHRLLAAFLLAVLPLQGAWAERADRNQPINIESDRLTAD
                     ENKQVAIFEGRVILTQGSFVLRADRLIVRQDDTGFEHATAYGAPATFRERREGTNEWI
                     DGEALRIEYDGRKEFVELFDKARLTREKDEVRGSYISYDARSDFFTVKSKPGEAPQEG
                     GDARVRAVIQPKSKEPPPASPPAQLQPSERLGGGAP"
     gene            3986..4735
                     /gene="lptB"
                     /locus_tag="VKZ48_07955"
     CDS             3986..4735
                     /gene="lptB"
                     /locus_tag="VKZ48_07955"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HLU77819.1"
                     /translation="MTAQAVNTRASRLHAANLNKRYKKRTVVHDVSLEVSSGEVVGLL
                     GPNGAGKTTCFYMIVGLVAVNGGRIYIDDRDITRMPIHQRARLGLSYLPQEASVFRRL
                     TVQENVRAVLELMNHEEEEIEQRLNGLLDELHIAHLRDATALSLSGGERRRVEIARAL
                     ATQPAFILLDEPFAGVDPIAVLDIQRIIRFLKERGIGVLITDHNVRETLGICDRAYII
                     NEGAVLASGKPEEIVYNENVRRVYLGEHFRL"
     gene            4809..6239
                     /locus_tag="VKZ48_07960"
     CDS             4809..6239
                     /locus_tag="VKZ48_07960"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014618027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HLU77820.1"
                     /translation="MKQTLQLKLSQHLTLTPQLQQSIRLLQLSTLELNQELEKFLADN
                     PLLEREEGDDDTTVPLTALSGNTAPAEEPVSETRAEAEPAYDEPRGGDMDWNFDGGGG
                     GLRDDEDTDYPQLPALSPTLRDHLMQQLLLTKLPARDRSLVALLIDALDEDGYLTQPL
                     EEIADMLPAELEVEVDELHIALRHLQHMDPTGVGARSPAECLALQLEALPPSTPGHAH
                     ALQIVREHLSLLASRDFLRLRRALSCDDAALRTAQRLIQSLNPRPGSEYGPLEARYVV
                     PDVIVRKIKGVWVASLNPDAMPKLRINRIYADILQNNRGAGPLAGQLQEARWLIKNVQ
                     QRFETIQRVSQAIVDRQRHFFEHGEVAMRPLVLREIADILSLHESTVSRVTTQKFMAT
                     PQGIFELKYFFGSHVATEAGGACSATAIRALIKQLVAAEDTKKPLSDSKISEILGQQG
                     IVVARRTIAKYREAMHIPPVSLRKSL"
     gene            6293..6613
                     /gene="raiA"
                     /locus_tag="VKZ48_07965"
     CDS             6293..6613
                     /gene="raiA"
                     /locus_tag="VKZ48_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HLU77821.1"
                     /translation="MNLSITGHNIEVTPALRSYVESKMERVLRRSDRVIDTNVVLRME
                     KVRHLAEAVMRVPGKDICVVGDEEDMYAAIDVMIDKLDRQLLKHKDRLTAHEHESVRR
                     VTEE"
     gene            6719..7204
                     /gene="ptsN"
                     /locus_tag="VKZ48_07970"
     CDS             6719..7204
                     /gene="ptsN"
                     /locus_tag="VKZ48_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HLU77822.1"
                     /translation="MNLIAKLLAPANILLDLEVSSKKRMFEQVGLLFENNQGIARSLV
                     FDSLFARERLGSTGLGQGVAIPHGRIKGLRESVGAFIRLSTPVAFDAPDGQPVHLIFV
                     LLVPEQATEQHLQILSELAQMFSDRELRQALSSATDPAALHQIITAWQPHVADQRRAA
                     L"
     gene            7173..8114
                     /gene="hprK"
                     /locus_tag="VKZ48_07975"
     CDS             7173..8114
                     /gene="hprK"
                     /locus_tag="VKZ48_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HLU77823.1"
                     /translation="MLQTSVAQLYEAKYDKLALTWLSGRAGGDALIRQDPTESAAFVG
                     HLNLIHPNRIQVLGKHEQAHLDALDATRLGSLIENCMAAHPAAILLADGVPGHPLLRQ
                     AAESRNIALWASPEPAAVLIDNLRRHLSKVLAESTTRHGVFMDVLGLGVLITGDSGVG
                     KSELGLELISRGHGLVADDVVEISRIAATQLVGRAPTMLKDFLEVRGLGVLNIRTIFG
                     ETAIRPKMNLRLMVHLARPNQPGGTPVERLPLHELSEDILGVTIRKVVIPVAAGRNLA
                     VLIEAAVRNYILQLRGYHSTEEFIERQRAAMGRDTQD"
     gene            8292..8483
                     /locus_tag="VKZ48_07980"
     CDS             8292..8483
                     /locus_tag="VKZ48_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HLU77824.1"
                     /translation="MKTVLALAVALTCFIGTAHAQDAKPTSQQEKMKWCNQQAKTKEL
                     KGDQRKAFMSECLRKEKKS"
     gene            8662..9504
                     /gene="rapZ"
                     /locus_tag="VKZ48_07985"
     CDS             8662..9504
                     /gene="rapZ"
                     /locus_tag="VKZ48_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HLU77825.1"
                     /translation="MQLILISGLSGSGKSIALNVLEDARYYCVDNLPAPLLFEAVGLL
                     RESGYERMAVSIDARSGTALTDLPAALLTLKSQGIDVRLLFLDANTETLIKRFSETRR
                     KHPLSDGNQTLAESIARERDLLQELVVLGHHIDTSDVAPNTLRAWIRDLVEVDRSGTT
                     LLFQSFGFKEGIPLDADLVFDVRCLPNPFYDPKLRPLCGMDREVCDFLEAIPDVASML
                     QDIGDFVERWLPHFLRDNRAYLTVAIGCTGGQHRSVYMVERLAERFQGQAPIVVRHRQ
                     LPPR"
     gene            9508..10146
                     /locus_tag="VKZ48_07990"
     CDS             9508..10146
                     /locus_tag="VKZ48_07990"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HLU77826.1"
                     /translation="MTSPERPSVQQVTVGLTGASGFIYGLRLIECLLRADIHVGVIYS
                     QAAQIVAKQELDLTLPTRPKEATAALASHFGIEGGRLAVYAREDWYAPVASGSNPADA
                     MVICPCSMGTLAAVANGLADNLIERAADVALKERRPLILVPRETPLSLLHLENMTRLA
                     RAGALILPANPGFWHRPQSIEAVVDYVVARVLDHLHVPHRLMERWGDSPSTA"
     gene            10160..10765
                     /locus_tag="VKZ48_07995"
     CDS             10160..10765
                     /locus_tag="VKZ48_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006048031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HLU77827.1"
                     /translation="MAQLTELEIPLFPLQTVLFPGGRLPLRVFEARYMDMVRDCLRND
                     LPFGVCLIKQGSEVGEVAVPEAIGCLATIAECDMQQLGVLNVRVSGGQRFRILDTHVA
                     DGNLLRGRVSLIAAEPDAAIPPRFLGCANLLRMIAADADKIQFDPPHRYEDAAWVGYR
                     LTEVLPVPLLAKQKLLELDDPISRLEILYRFLEQRGLLASR"
     gene            complement(10712..11800)
                     /gene="mutY"
                     /locus_tag="VKZ48_08000"
     CDS             complement(10712..11800)
                     /gene="mutY"
                     /locus_tag="VKZ48_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HLU77828.1"
                     /translation="MSAFAQALIAWQQEHGRHDLPWQRTRDPYAIWLSEIMLQQTQVA
                     TVIPYYQRFRERFPDIASLAAADEDEVLRLWSGLGYYSRARNLHRAAQRIVVQHGGVF
                     PVALADIEALPGIGRSTASAIAGFAYGARAAILDGNVKRVLTRHFGVGGFPGERAVEQ
                     RLWALAESLLPADGIEAYIQGSMDLGATICLPKRPVCNRCPVSGTCIAFTENRVHELP
                     SPRPRKALPTRETAMAIYLHAGEVLLQKRPAVGIWGGLWCFPEAVDEEEVRRAGDRLY
                     GCEVARTERLAVLKHSFTHFHLHITPVCAEVKRRLPRAAQPGVMWLRIEDALGGALPT
                     PVKKILRALGGSTRGKQAALFEEAVEDL"
     gene            complement(11800..14163)
                     /locus_tag="VKZ48_08005"
     CDS             complement(11800..14163)
                     /locus_tag="VKZ48_08005"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HLU77829.1"
                     /translation="MKILKWLLWIVVAVLVLAIGLGTYLALTFDPNEYKPELIKLVKE
                     RTGRTLSIDGDIELKFFPKIGAGVEGVALSVPNGDARFAQVQEARVALALLPLLSKQI
                     IVDEVTLTGLRAELVKYRDGSTNFDDLLGGGKPPKQKDEPQKTEPAAKQPMAIDIGGI
                     ALQDADVTWRDEASGMQLRAADVNLNTGRIASGVPGQMRFSARIEGKEPELALQIELS
                     AGYRLDMETGGAQLSNVDLKAEGDAAALRALRLHVKGDTAGWNPQTALIDLNGIEASA
                     KSGDMLDAQFSIPKLHISADRSESKPINGRLQYRTPELEVAADLALAALQTEGEQVRF
                     SRVEADMKVKQHELGIDGKVATPVLLDLGAKRLQLPQIAGDFVFSGPDVPNKSAKLRL
                     EGAKTLDWGAESAKADLIAKLEDGTVNLRAAIKGFDKPAVDFDLQADRLDVDRYLPPG
                     KETGAKGGASKPAATPGASAGGATEEAPIDLAWLKPLQAKGSIQVGELVVSRIHAQDV
                     DIRINADKGKLSVEPMSARLYQGSLQGSTQIDANRNSYVVKQRLVGINIGPLLRDAVQ
                     KDILEGRGTVQLDLRTVGTTATGLKRGLNGTASVVLKDGAVKGINLAAALRKAKAMLG
                     SKSAAEEQAQGGEQTDFSDMSASFRIDKGVAHNKDLLLRSPFLRVQGAGDIDLVAASM
                     DYDVKASLVNTSTGQSGKKLDEVASLTVPVAISGPLENLSYRLDTRALVTDQAKDALR
                     EQLQRRLGGNDKEEQATEGATGKADKPAAEEKPRGKIGEQLLRGLLK"
     gene            complement(14160..15080)
                     /locus_tag="VKZ48_08010"
     CDS             complement(14160..15080)
                     /locus_tag="VKZ48_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HLU77830.1"
                     /translation="MPATFDGKLVVAISSRALFDLEESNRIFEEEGVDAYFQYQRERE
                     DEVLAPGIAFPLVKKLLKLNTLPPARPRVEVILLSRNTADTGLRVMNSICHYQLDITR
                     AAFTGGESTWSYVPAFQADLFLSANPVDVRKALDAGNAAATILPSNVGQNLTDSDQLR
                     IAFDGDAVIFSDEAERVYKRDGLDRFHESEASAARDPLSGGPFKNFLSALHRIQSEYP
                     SDASPIRTALVTARSAPAHERVVRTLRAWNIRIDEALFLGGLAKGDFLRVFGADIFFD
                     DQRGHCESARLHVATGHVPFGVANENKEGA"
     gene            15222..15295
                     /locus_tag="VKZ48_08015"
     tRNA            15222..15295
                     /locus_tag="VKZ48_08015"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15254..15256,aa:Gly,seq:ccc)
     gene            complement(15612..16511)
                     /locus_tag="VKZ48_08020"
     CDS             complement(15612..16511)
                     /locus_tag="VKZ48_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018574772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLU77831.1"
                     /translation="MDRVTGMRVFVRAALDGSLSAAARHLGMSPAMAAKHVNALEARL
                     GVKLFHRTTRRLSLTAAGSDYLEACQRILPEIDEAEAAVASQRVKASGLLRMNVPLSF
                     GSRFIAPLMPAFSRLYPEVKVELGLSDAQLDLIAGSWDLAIRIGQLADSPLQARRLGD
                     SAMHVCAAPAYLDRRGVPRRIGELTQHNCLSYTLSAMQSGKHWAFGAQGEHRVPVSGD
                     LMANNGDALLAAAVRGQGIIYQPHFIVGEALARGELVALELDKPVVELGGIHALYPPD
                     RRPPAKVRVMIDYLVEAFAQTPP"
     gene            16613..>17415
                     /locus_tag="VKZ48_08025"
     CDS             16613..>17415
                     /locus_tag="VKZ48_08025"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HLU77832.1"
                     /translation="MRHDTPTPAQTRMPVYFVPHGAGPCFFMDWNPADAWRQMAAFLK
                     NIAATLPARPRAVLVISGHWLTPQYAVTGHARPSLIYDYYGFPPHTYELTYPAPGDPA
                     LAAQVAERLEQNGLASRVDPDRGFDHGVFIPLKLMFPNADIPLVQVSLRQDLDPAAHL
                     AAGRALAALRDEGVLIVGSGMSFHNMRGYGDPRYTPLSAEFDDWLTAAVTSPAEERDT
                     RLQHWTEAPHAHQCHPAGHEEHLIPLLVAAGAAMESMGQRVYTEQIMETT"
BASE COUNT         3180 a         5786 c         5374 g         3075 t
ORIGIN      
        1 aggggggcac ggcgtcgtca cgacatggga acgtcagtat catgaaggcg gtttggatgc
       61 gctcaaaccc aagccccgag gacgccaaaa gatgtcagtt gccccgcaat cgcccaagcc
      121 gttaccgcct tctgccgagg aggctcgcgc cctggagcag ttgcgcaagg agaatgaata
      181 tctgcgcgcg gaggtggcgt acctaaaaaa gttgagggcc ttgcgtcagg aaaaagagct
      241 ggccgcgccg aaaaaacgcg ggtagtgctc caactgaggc aaggccacga tctgtcggtg
      301 ctactgaagg tggcgggact gtcgcgcagt acgttctact accaagtcaa agtgcagcag
      361 gttggtgacc ggcatgccgg cttgaagtcg cgtatccgcg ttctctatga gcgccataaa
      421 ggccgctacg gctatcggcg catcacggcg gcgctgcgcc aggccggcga ggtggtcaac
      481 cacaagacgg tgcagcgctt gatgcaagcc atgggactga ggtcactggt gcgaccgaaa
      541 aaataccgtt cttatcgggg ccagcaggcc gaagtgccca acctcctggc gcgccagttt
      601 caggctgaac ggccaagcca gaagtgggta accgacgtga cgcagttcaa tgtccgcggc
      661 cagaagttgt atctctcacc agtgctggat ctctacaacg gggaaatcgt ggcctacgag
      721 atgcgccaga agcccttctt cccgttggtg ggtcgcatgc tcagaaaagc actggccaaa
      781 ctggtagacc aagacacgcc attgttgcac tcggatcagg gctggcaata ccagatgccg
      841 gtctatcggc gacaactggc cgaacgcggc ttgacccaga gcatgtcgcg caaaggcaac
      901 tgcctggaca acgctgtcat ggagagcttc ttcggtacgc tgaagtccga gttcttctac
      961 ttgaaccgct ttgacagcgt cgaacaacta cggaacggca tccgtcgcta catctactac
     1021 tacaatcacc accgcatcaa gaccaagtta aaaggcctga gtccggtgca ataccgaacc
     1081 caggcctttt ctacttagcc cgtcttttaa ctgtccaatt tcttggggtc agttcactcg
     1141 gcgggctttt tctttgcccg cttcctgcgc gggcggccca tttccccggc gaccggcgtc
     1201 cgccgcttgc tatacttttt cgcatgacca aaactgctcc ccccgcggcc gccgacaccc
     1261 cggcggcgct acgccttgcg cgcgacgtcc tgcagatcga ggccgctgcg atcacggcgc
     1321 tcgtggagcg cctggacgac actttcgtgc gcgccgtcca actgattctg gactgcgaca
     1381 gccgggtggt agtgagcggc atcggcaagt ccggccacgt cggacgtaag atcgcgtcca
     1441 cactcgccag cacgggaacg cccgcctttt tcgtccaccc ggccgaggcg agccatggcg
     1501 atctgggcat gatcacccgt cgcgacgtga tgatcgcgat ttcctattcg ggcgagagcg
     1561 acgagttgct gaccatcatc ccgctggtga agcgccaggg cgcgaagctc atcgccatga
     1621 ccggcaaccc gcaatccacg cttgccgccg aggcggacgt ccacctcaac gtccatgtgg
     1681 ccaaggaggc gtgcccgctg aatctcgcgc ccactgccag caccacggcg acgctggcgc
     1741 tgggcgatgc gctcgccgtg gcgctgctcg atgcgcgcgg cttcaaggcg gacgatttcg
     1801 cccgctccca tccgggaggc agtctggggc ggcggttgct gacgcacgtg aacgacgtca
     1861 tgaagcgcgg caaggacgtg cccgccgtgc ggctgggcgc gccgctgacc gatgccattc
     1921 tggaaatctc gcgcaagggc atgggcatga ccgcgattgt cgacgagcat gaccgtgtgc
     1981 tggggatctt caccgacggc gacctgcgcc gcgcggtgga gcgcggcgtg gatctgcgcg
     2041 gcgcagcgat cagcgaagtc atgacaccct cgccgctcac catcgccgcg accgagcttg
     2101 cggtcgaagc cgtggccctc atggaaaacc ggcggatcaa ccaactgctg gtcaccgacg
     2161 cggaacagaa gctgatcggc gcgctgaaca tgcacgattt gtttcaggcg aaggtcgtct
     2221 gagcatggac cggaccgagg cactcgcgcg ggcgcgcaac atccggctga tgagtttcga
     2281 cgtggacggc gtactgaccg acggtacgct ttatctcagc gatgcgggcg aggaaatcaa
     2341 gggtttcaat accctcgacg gcctcggtct gaagctgttg caggataacg gtgtggagct
     2401 tgcgatcatt accggccgcg aatccaaact ggttgccctg cgcgcgcaga acctcggcat
     2461 ccggcggctg catcagggcg tggaacggaa gctcgagatc ttcgatgccc tgcgcgccga
     2521 actcggtctt tccctggagc aatgcgggta catgggcgac gacctgcctg atctgcccat
     2581 cctcacgcgt tgcggctttg ccgtcaccgt gccgcaggcg cccgcagcgg tccgcgcgcg
     2641 cgcgcattac gtcaccgaag cgcgcgccgg cgccggcgcc gcgagggagg tgtgcgaatt
     2701 cattctgcag gcgcaaggcc ggctcgaaca ggcgatcgcc ccctacctcg catgaagccg
     2761 cggtccagca cgctgtttcc cctggttctg atgatcgggc tggcgctggt cagcctgtgg
     2821 ctcgaacacg ccgttcgcgg cggcgatcag agcgcccact ccgcgctacg ccacgacccg
     2881 gacttcatca tcgagaattt caccaccacc gacatcggcg gacagggtcg cgcgcgcgcg
     2941 accctgcgcg ccaaacggat ggtgcactac cccgacaacg acaccagtga actggaggag
     3001 ccgtacctgg tccatctgaa tcccgaccgc cccccgctgc acatcagcgc cgagcgcgga
     3061 ctggtggccg gcaccggaga ggaagtggag cttttcgaca acgtggtggt gcggcgtctc
     3121 gcagcggggc agcaagccga gttgcggatc cagacgtcct atctgcaggt ctttccggac
     3181 aagcatctgg cccgcacgcc tgccgaagtg ctcattaccg agggcgcttc gcgcctgaaa
     3241 ggcgtcggca tggaagtgaa cagcagcacc gggcatctca aactgcagtc caaggtcagc
     3301 ggcttttacg taaaatcggg cgctagtagc ggcaagtaag cgccgctttt ttcagttctg
     3361 cctatatcgc gatcaacgtg cccgtctatt tccctcatcg cctccttgcg gcctttctac
     3421 tggccgtgct gccgctccag ggggcgtggg cggagcgtgc ggaccgcaat cagccgatca
     3481 acatcgagtc cgatcggctc accgcagacg aaaacaagca ggtggcgatt ttcgagggtc
     3541 gcgtgattct cacgcagggg agcttcgtac tccgtgccga tcggctgatc gtgcgtcagg
     3601 acgacaccgg cttcgaacac gcaaccgcct acggtgcgcc cgccaccttc cgcgagcgcc
     3661 gcgaaggcac taacgaatgg atcgacggcg aagccttgcg catcgagtac gacggccgca
     3721 aggaattcgt cgagttgttt gacaaggcac gcctgacgcg cgagaaggac gaagtgcgcg
     3781 gaagctatat ctcttacgac gcgcgctcgg atttttttac ggtgaaaagc aaacccggcg
     3841 aagcgccgca ggaaggcggc gatgcacgcg tgcgcgccgt cattcaaccc aagtccaagg
     3901 agccgccgcc cgccagccca ccggcacagt tgcagccgtc cgaacgactc ggaggcggcg
     3961 ccccgtgaac gcaccggacc ccacgatgac ggctcaggca gtgaataccc gcgccagtcg
     4021 cctgcatgcg gccaatctga acaagcgcta caagaagcgc accgtcgttc acgacgtatc
     4081 gctcgaagtg tcgagcggcg aggtggtcgg tctgctcgga ccgaacggcg caggcaagac
     4141 gacctgcttc tacatgatcg tcggcctggt ggcggtgaac ggcggccgca tttacatcga
     4201 cgatcgcgat atcacgcgca tgcccattca tcagcgcgcg cgtctcggcc tgtcctatct
     4261 gccgcaggaa gcttcggtat tcagacggtt gaccgtgcag gagaacgtac gcgccgtgct
     4321 cgaactgatg aaccatgaag aagaggagat cgaacagcgc ctcaacggtc tgctcgacga
     4381 actccacatc gcgcacttgc gcgacgccac cgcgttgtcg ctgtccggcg gcgaacgccg
     4441 gcgagtggaa atcgcccgcg cactggcgac gcaaccggcc ttcattctgc tcgacgaacc
     4501 cttcgccggc gtggacccca ttgccgtcct ggacatccag cgcatcatcc gctttctcaa
     4561 ggagcgcggc atcggcgtac tcatcaccga ccacaacgtg cgggaaacac tgggcatctg
     4621 cgatagagcg tacataatta acgaaggtgc cgtgctggcc tccgggaagc cggaggagat
     4681 tgtttataat gaaaatgtcc gtcgggtcta cctgggcgaa cacttccgcc tttagcgctc
     4741 ggggcgcgca ccggcggact cgcaaggccc gcagtccggc cctcccaagt ccctagtccg
     4801 cggtatcgat gaaacaaact ctgcaactca agctttctca gcacctgacg ctgacgccgc
     4861 agttgcagca gtccatccgt cttctgcagc tttccaccct cgagctgaac caggaactgg
     4921 aaaagtttct ggccgacaac ccgctgctcg aacgcgagga aggcgatgac gacaccaccg
     4981 ttcccctgac ggcgctgtcc ggcaataccg cccccgccga ggagcccgtg tcggaaaccc
     5041 gggccgaggc cgaacccgcc tacgacgagc cgcgcggcgg cgacatggat tggaatttcg
     5101 acggcggcgg cggcggactg cgcgatgacg aagataccga ttacccgcag ctgccggcgc
     5161 tgtcgcccac gttgcgcgat cacctgatgc agcagttgct gctgaccaaa ctgccggcac
     5221 gcgaccgcag tctggtcgca ctgctcatcg acgccctgga tgaggacggt tacctcaccc
     5281 agccgctgga ggaaatcgcc gacatgctgc cggcggaact cgaagtggaa gtcgatgagc
     5341 tgcatatcgc cttgcgtcac ctgcagcaca tggaccccac tggggtgggc gctcgctctc
     5401 cggcggagtg cctcgccttg cagctggagg ccctgccgcc gagcacaccg ggccacgcgc
     5461 acgcgctgca gatcgtgcgc gagcatctgt cgctgctcgc ctcgcgcgat ttccttcgtc
     5521 tgcggcgcgc gctcagttgc gacgatgcgg ccctacgtac ggcgcagcgg ctgatccaga
     5581 gtctcaaccc ccgcccgggc agcgagtacg gtccgctgga ggcgcgttat gtcgtgcccg
     5641 acgtgatcgt gcgcaagatc aagggcgtat gggtggcgag cctcaacccg gatgcgatgc
     5701 ccaagctgcg catcaaccgc atctacgccg acatcctgca gaacaaccgc ggcgccgggc
     5761 cgctggcggg ccagttgcag gaagcgcgct ggctgatcaa gaacgtgcag cagcgcttcg
     5821 agaccatcca gcgcgtctcg caggccatcg tcgaccgtca gcggcacttc ttcgaacacg
     5881 gcgaggtggc catgcgtccg ctggtgctgc gcgaaatcgc cgatattttg agcctgcacg
     5941 aatccacggt ctcgcgcgtg accacgcaaa aattcatggc gacgccgcaa ggcatcttcg
     6001 agttgaagta cttcttcggc agccatgtcg cgacggaagc cggcggagcc tgctcggcca
     6061 cggcgatccg cgcgctgatc aagcaactgg tcgccgccga agacacaaag aagccgcttt
     6121 cggacagcaa gatttcggag atactcggtc agcagggcat cgtcgtggcg cgtcgcacca
     6181 tcgcgaaata ccgcgaagcg atgcacattc cgccggtaag cctgcgcaag tcgctttaat
     6241 cgagcgcgag ccccgctcgc gcaccgcagc tgatacagcg aggagagcaa gcatgaattt
     6301 gagcatcacc ggacacaaca tcgaagtcac ccccgctctg cgcagctacg tcgaaagtaa
     6361 aatggagcgg gtcttgcgcc gctccgatcg ggtcatcgac accaacgtgg tgctgcgaat
     6421 ggagaaggtg cgccacctgg ccgaggcggt catgcgcgtc ccgggcaagg acatttgcgt
     6481 cgtgggggat gaagaggaca tgtacgccgc cattgacgtc atgatcgaca agctcgatcg
     6541 gcaattgctc aagcacaagg accgcttgac cgcgcatgaa cacgagtcgg tgcgccgggt
     6601 gaccgaggaa taacgcaggt acggggggcg cgagtagcgg ctataatcgc gccctttggc
     6661 cccggcgcac ggcttgcatc ggggcccttc tgaagctctt gcagaccgga agtaagagat
     6721 gaatctcatc gcgaagctgc tcgcgcccgc caatattctt ctcgatctcg aggtgagcag
     6781 caaaaaacgg atgttcgaac aggtggggct cctgttcgag aacaatcaag gcattgcccg
     6841 cagcctggtg ttcgacagcc tgttcgcgcg cgagcggttg gggtccaccg gactcgggca
     6901 gggtgtagcc atcccgcatg ggcgcatcaa gggcctgcgc gagtcggtcg gtgccttcat
     6961 ccggctgtcg accccggtgg ccttcgatgc gccggacggt caaccggttc acctcatttt
     7021 tgtcttgctg gtacccgaac aggcgaccga gcagcatttg cagattctgt ccgaactcgc
     7081 gcaaatgttc agcgaccgtg aactgcgtca ggcgctgtcc agcgcgaccg acccggccgc
     7141 cctgcaccag atcatcactg cctggcaacc gcatgttgca gaccagcgtc gcgcagcttt
     7201 atgaggcgaa atacgacaag ctcgcgctga cctggctttc cggtcgcgcc ggcggcgacg
     7261 cgctcatccg ccaggatccg accgaatcgg ctgccttcgt cgggcacctc aacctcattc
     7321 atccgaaccg tatccaggta ctgggcaagc atgagcaggc ccatctggat gcgctcgatg
     7381 caacgcgact gggatcgctg atcgaaaact gcatggctgc ccatccggca gccattctgc
     7441 tggccgacgg cgtgcccggc catccgctgc tgcggcaggc ggccgaatcc cgaaacatcg
     7501 cgctctgggc ctcgcccgag ccggcagccg tgctgatcga caatctgcga cgccatctgt
     7561 cgaaggtgtt ggcggaatcc accacacgcc acggcgtgtt catggacgtc ctgggcctgg
     7621 gcgtgctgat caccggcgat tcgggcgtgg gcaagagcga actgggcctg gaactgatca
     7681 gccgcggcca cggactcgta gcggacgacg tggtggagat ttcgcgcatc gccgccacgc
     7741 aactggtcgg ccgcgcgccg accatgctca aggacttcct cgaagtgcgc ggcctgggcg
     7801 tactcaatat ccgcaccatc ttcggcgaga cggccatacg gccgaaaatg aatctgcgcc
     7861 tcatggtgca tctggcgcgc cccaaccagc cgggcggcac gccggtcgaa cggctgccgc
     7921 tgcacgagtt gtcggaagac atcctcggcg tgacgatccg caaggtggtc attcccgtcg
     7981 ccgccggccg caacctggcc gtactgatcg aagcggcggt gcgcaactac atcctgcagt
     8041 tgcgcggcta tcacagcacc gaggaattca tcgaacgtca gcgcgccgcc atggggcgcg
     8101 acacgcagga ctgactgtcg ccgttgtcgc gcgatctttc acttaaagtc gccgatcgtt
     8161 taccggatca ttcttgatcc gcgcccgtat gccctttccc ggcctaagcc atcgaggcta
     8221 tcggcgccgg ctacccgctc gtttcaacat acgcctccct tcagggaatt cactcaatca
     8281 agggaggcga tatgaagaca gtcttggctc tggcagtggc attgacttgt ttcatcggca
     8341 cggcgcatgc gcaggacgcc aagccgacct cgcagcagga gaaaatgaag tggtgcaatc
     8401 agcaggcgaa aaccaaagag ctcaagggcg atcaaagaaa ggcgttcatg agcgaatgcc
     8461 tgcgcaagga aaagaaaagt tgagcgcgac attgcgcgac ccaagggcgc ctcggcgccc
     8521 ttttttatcg cccgcagacg cgatcgctcg ctacggttaa gcgcagttcc cgagcgcgtc
     8581 gggcagcccg caagccctcg ccacaggcga aaatttcccg attttgccgg cctatgacgc
     8641 atgggctatg cttgcccggc tatgcaactc atcctcatca gcggcctgtc cggatcgggc
     8701 aaaagcatcg ccctcaacgt gctggaggac gcgcgctact actgcgtgga caacctcccc
     8761 gcgccactgc tgttcgaggc ggtgggcctg ctgcgcgagt ccggttatga gcgcatggcc
     8821 gtcagcatcg atgcccgcag cggcacggcg ctgaccgacc tgcccgccgc gctgctgacc
     8881 ctgaaaagcc agggcatcga cgtccgcctg ctgtttctcg acgccaacac ggaaacgctg
     8941 atcaaacgct tttccgaaac ccgtcgcaaa catccgctct cggacggcaa ccagacactg
     9001 gccgagagca tcgcgcgcga gcgcgacctg ctgcaggaac tggtagtgct cggccatcac
     9061 atcgacacca gcgatgtcgc gccgaacacc ctgcgcgcct ggatccgcga cctggtcgaa
     9121 gtcgaccgat cgggcaccac gctgctgttc caatccttcg gcttcaagga aggcattccg
     9181 ctggatgccg acctggtctt cgacgtgcgc tgcctgccga accctttcta cgatcccaaa
     9241 ctacggccgc tctgcggcat ggatcgcgaa gtctgcgact ttctcgaagc cattcccgat
     9301 gtcgccagca tgctgcagga catcggcgat ttcgtggagc gctggctgcc gcacttcctg
     9361 cgcgacaacc gcgcctatct caccgtcgcc atcggctgca ccggcgggca gcaccgctcc
     9421 gtgtacatgg tggaacgcct ggccgagcgc ttccagggac aggcacccat tgtcgtgcgg
     9481 caccgtcaac tgccgccgcg ctgacgcatg acttcgcccg agcgcccttc cgtccaacag
     9541 gtcaccgtcg gtctcaccgg cgcttccggg ttcatctatg ggttacggct gatcgaatgc
     9601 ctgctgcgcg cggacatcca cgtgggcgtg atttacagcc aggcggcgca gatcgtcgcc
     9661 aagcaggaac tcgatctcac cctgcccacg cgccccaagg aggcgacggc cgcgctcgcc
     9721 tcgcatttcg gcatcgaagg cggccggctg gcggtgtatg cacgcgagga ctggtacgcg
     9781 ccggtggcct ccggctccaa cccggccgat gccatggtga tctgcccctg ctccatgggc
     9841 acgcttgcgg cagtcgccaa cggcctggcc gacaacctca tcgaacgtgc agccgacgtg
     9901 gcgctgaagg aacgccggcc gctcattctc gtgccgcgcg aaacgccgtt gtcgctgctg
     9961 catctggaaa acatgacacg actggctcgc gccggcgccc tcattcttcc cgccaatcca
    10021 ggtttctggc atcgaccgca aagcatcgag gcggtcgtgg attatgtcgt cgcgcgcgtg
    10081 ctcgaccact tgcacgtccc gcaccgactg atggagcgtt ggggagattc tccctccacc
    10141 gcataaggct ctccaccgca tggctcaact caccgagctc gaaattccgc tgttcccgct
    10201 gcagaccgta ctgtttccgg gcggacgcct gcccttgcgc gttttcgagg cgcgctatat
    10261 ggacatggtg cgcgactgcc tgcgcaatga ccttccgttc ggcgtgtgcc tcatcaagca
    10321 gggcagcgaa gtcggtgaag tcgccgtgcc ggaagcaatc ggctgtctgg ccaccatcgc
    10381 cgaatgcgac atgcagcaac tgggcgtgct caatgtgcgc gtcagcggcg gccagcgctt
    10441 tcgcatcctc gacacgcatg tcgcggacgg caacctgctg cgcggcaggg tctcgctgat
    10501 cgcagccgag ccggatgcgg ccattccgcc gcgcttcctc ggttgcgcca acctgttgcg
    10561 gatgatcgcc gccgacgccg acaagatcca gttcgacccg ccgcatcgtt acgaggacgc
    10621 ggcatgggtc ggttatcgcc tcacggaagt gctgccggtg ccgctgctgg ccaaacagaa
    10681 gctgctcgaa ctggacgacc cgatatcgcg actagagatc ctctaccgct tcctcgaaca
    10741 gcgcggcctg cttgcctcgc gttgagccgc ccagcgcgcg caggattttc ttcaccggcg
    10801 tgggcagtgc gccgccaagt gcgtcctcga tacgcagcca catgacgccc ggctgcgccg
    10861 cgcgcggcag gcgccgcttc acttccgcgc ataccggcgt gatgtgcaga tgaaaatgcg
    10921 tgaagctgtg cttcagcacg gccagccgct ccgtgcgtgc gacctcgcag ccgtacaggc
    10981 gatcgcccgc acgacgcact tcttcctcat cgaccgcttc ggggaaacac cacaggccgc
    11041 cccagatgcc gaccgccgga cgcttctgca gcagcacttc gcccgcatgc agatagatcg
    11101 ccatggcagt ctcgcgtgtg ggcagcgcct tgcgcgggcg cggcgagggc agttcatgca
    11161 cgcggttttc cgtgaatgcg atacacgtcc cggaaacggg acagcgattg cagaccggac
    11221 gcttcggcag gcagatggtc gctcccaggt ccatcgagcc ctggatatag gcttcgatcc
    11281 cgtccgccgg cagcaacgac tccgccagtg cccatagccg ctgttcgacc gcacgctcgc
    11341 cgggaaagcc tcccacgccg aagtggcgcg tcagcacgcg cttgacgttg ccgtccagaa
    11401 tggcggcacg cgcgccgtag gcgaacccgg cgatcgccga cgcggtggag cgcccgatgc
    11461 ccggcagcgc ttcgatatcg gccagcgcaa cgggaaagac gccgccatgc tgcacgacga
    11521 tgcgctgcgc cgcccgatgc agattgcgcg cacgcgagta gtagcccagg ccgctccaca
    11581 gacgcagcac ttcatcctcg tccgcggcgg ccaggctggc gatgtcggga aagcgctcgc
    11641 gaaagcgctg gtagtaggga atgaccgtgg ccacctgcgt ctgctgcagc atgatttccg
    11701 acagccagat ggcgtaggga tcgcgcgtgc gctgccacgg cagatcgtgc cggccgtgtt
    11761 cctgctgcca cgcgatgagc gcttgcgcga acgccgacat tacttcagca gaccgcgcag
    11821 cagttgctcg ccgatcttgc cgcgcggctt ttcttcggcg gcgggcttgt ccgctttgcc
    11881 ggttgcgcct tcggtcgcct gctcctcttt gtcgttgccg cccaggcgcc gctgaagttg
    11941 ctcgcgcagc gcatccttgg cctggtcggt caccagcgca cgcgtatcca ggcgatagct
    12001 gaggttttcc agcgggccgc tgatggccac cggcaccgtg aggctcgcga cttcgtcgag
    12061 cttcttgccg gactgcccgg tggaggtgtt caccagcgaa gccttgacgt cgtaatccat
    12121 gctcgcggca acgaggtcga tatcgcccgc gccctgcacc cggagaaagg gcgaacgcag
    12181 gagcagatcc ttgttgtgcg cgacgccctt gtcgatgcgg aagctcgcgc tcatgtctga
    12241 gaagtcggtt tgctcgccgc cctgcgcctg ctcttcggcg gcgctcttcg agccgagcat
    12301 tgccttggcc ttgcgcagcg ccgcagcgag gttgatgccc ttcaccgcgc cgtccttcag
    12361 cacgacgctg gccgtgccgt tcaggccgcg cttcaaaccg gtggcggtcg tgcctaccgt
    12421 acgcaaatcg agctgtaccg tgccgcgtcc ttccaggatg tccttctgca ccgcgtcgcg
    12481 cagcagcggg ccgatattga tgcccaccag acgctgcttg acgacatagc tattccggtt
    12541 cgcgtcgatc tgcgtgctgc cctgcaggct gccctggtag aggcgcgccg acatcggctc
    12601 gaccgacaac ttgcccttgt cggcgttgat gcggatatcg acatcctgcg catggatgcg
    12661 cgaaacgacc agttcgccca cctggatgct gcctttcgcc tgcaacggtt tgagccaggc
    12721 cagatcgatc ggcgcctctt ccgtcgcgcc gcccgcgctc gcacccggtg tcgctgcggg
    12781 tttggacgca ccacccttcg cgcccgtctc cttgcccggc ggcagatagc gatcgacgtc
    12841 gagacgatcg gcctgcagat cgaaatcgac cgcgggtttg tcaaaacctt tgatcgcggc
    12901 gcgcaagttc actgtgccgt cctcgagctt ggcgatcaga tcggccttgg ccgactcggc
    12961 gccccagtcg agcgtcttgg cgccttcgag acgcagcttg gcgctcttgt tgggcacatc
    13021 gggaccggag aaaacgaagt cgccggcgat ttgcggcagt tgcagccgct tcgcgccgag
    13081 gtcgagcagc acgggcgtcg ccaccttgcc gtcgatgccc agttcgtgct gcttcacttt
    13141 catgtcggct tcgacgcggc tgaagcggac ctgttcgcct tcggtctgca aggccgccaa
    13201 tgccagatcg gcggcgactt cgagttccgg cgtgcggtac tgcaaccgcc cgttgatcgg
    13261 cttgctttcc gagcggtcgg cggagatatg aagtttcgga atgctgaact gcgcgtccag
    13321 catgtcgccc gatttggcgc tcgcctcgat cccgttcagg tcgatgagcg cggtctgcgg
    13381 attccagccc gccgtgtcgc cttttacatg caggcgcaac gcgcgcaagg cggccgcgtc
    13441 gccttcggcc ttgagatcga cgttcgacaa ctgggcgcca cccgtttcca tatcgaggcg
    13501 gtagcccgcg gacagctcga tctgcagcgc cagttcgggc tccttgccct cgatgcgtgc
    13561 ggaaaagcgc atctgtcccg gcacgccgct ggcgatccgc ccggtattga gattcacgtc
    13621 ggcggcgcgc aactgcatgc cgctcgcttc gtcgcgccac gtcacatcgg cgtcctgcag
    13681 tgcaatcccg ccgatgtcga tggccatcgg ctgcttggcc gcgggctcgg ttttctgcgg
    13741 ttcgtctttc tgtttcggcg gtttcccgcc gcccagcaga tcgtcgaagt tggtggagcc
    13801 gtcgcgatac ttcaccagtt ccgcccgcag gccggtcagc gtgacctcgt ccacgatgat
    13861 ctgcttggac agcagcggca gcagcgccag cgccacgcgc gcttcctgca cctgcgcgaa
    13921 gcgtgcgtcg ccgttcggca ccgacagcgc gacaccctcg acgcccgcgc cgatcttggg
    13981 gaagaacttc agctcgatgt cgccgtcgat gctgagcgtg cggcccgttc gctccttgac
    14041 cagcttgatc agctcgggct tgtattcgtt ggggtcgaag gtgagcgcga ggtatgtgcc
    14101 cagaccgatc gcgaggacca atacggcgac gacgatccac aacagccact tcaatatctt
    14161 catgcacctt ccttattctc gttggcgacg ccgaagggca catgcccggt tgcaacgtgc
    14221 agccgcgcgg attcgcagtg tccgcgctga tcgtcgaaaa agatgtccgc gccgaacacg
    14281 cgcagaaaat cgccttttgc caggccgccg agaaacaacg cttcgtcgat gcggatattc
    14341 cacgcgcgca aggtgcgcac gacgcgctcg tgtgcgggcg cgctgcgcgc cgtcaccagc
    14401 gccgtgcgga tcggcgacgc atccgacgga tattcggact ggatgcggtg cagggcgctg
    14461 agaaaattct tgaacggccc gccgctgagc ggatcgcgtg cggcggacgc ctcgctctcg
    14521 tgaaagcggt cgaggccgtc ccgcttgtaa acccgctccg cctcatccga gaagatgacc
    14581 gcgtcgccgt cgaaagcgat gcgcaactgg tcgctgtcgg tgagattctg ccctacgttg
    14641 gagggcagga tcgtcgccgc cgcattgccg gcatccagcg ccttgcgcac atcgaccgga
    14701 ttggccgaca ggaaaagatc ggcctggaac gccggcacgt aggaccaggt gctttcgccc
    14761 ccggtaaacg ccgcgcgcgt gatgtcgagc tggtaatggc agatcgaatt catcacccgc
    14821 agaccggtat ccgcggtatt gcgcgacagg aggatcactt ccacacgcgg acgcgcgggc
    14881 ggcagcgtat tgagcttcaa cagcttcttc accagcggga aggcgatgcc gggcgcgagc
    14941 acctcgtctt cgcgctcgcg ctggtactgg aaatacgcgt cgacgccctc ctcctcgaag
    15001 atgcgattgc tttcctcgag atcgaacagc gcgcgcgacg aaatcgccac aacgagtttg
    15061 ccgtcgaaag ttgcgggcat ggtatatccg aatccgataa cgagccgcat gcggcccgcg
    15121 atgcagtcat tctaaccttc atgcatgcgc ttcttcgatg ccgccgttgc ccgtacacga
    15181 gccgctcgca tagaatcagc gcagcccaag cgctacccga agcgggtgta gttcaatggt
    15241 agaacggcag cttcccaagc tgcatacgtg ggttcgattc ccatcacccg ctccagattc
    15301 gaatgtcacc ttactaggcg gcgttcgcat ttgggacgga cgacgggtga agtacccacc
    15361 ggttcgacaa ccggaggtcc gggggacgtc cggttggacg ccaaggcgcc ccagcggctt
    15421 ggcggtcccg agcagcacgc tgcgagggat gagcggatcg cctcgagcga gacgctcaca
    15481 ttcccatcac ccgctccaac gccgcctttc gaggcgggcc gccacaccgc aaaatccccg
    15541 ctcagcctgc gcggcacgaa aagccgcatg cgcggaaaca gcgatgcccc gacttcatcg
    15601 ccgaaagcgc atcagggcgg cgtctgcgca aacgcctcca ccaggtaatc gatcatcacc
    15661 cgcaccttgg ccggcggccg ccggtcgggc ggatacagcg catggatacc gccgagttcg
    15721 acgacgggct tgtccagttc cagcgccacc agctcgccgc gcgcaagcgc ttcgccgacg
    15781 atgaaatgcg gctggtagat gatgccctgt ccgcgcaccg ctgcagccag cagtgcgtct
    15841 ccgttgttgg ccatcagatc gccgctgacc ggcacccgat gctcgccctg cgcaccgaat
    15901 gcccaatgct tgccgctttg catggcggaa agggtatagc tgaggcaatt gtgttgcgtc
    15961 agttcgccga tccttcgggg cacgccccgg cgatctagat aagcgggcgc ggcgcagacg
    16021 tgcatcgcgc tgtcccccag acggcgcgcc tgcagcggac tgtcggccag ctggccgatg
    16081 cggatcgcca gatcccaact gccggcgatc aaatcgagtt gggcgtcgct gagacccagt
    16141 tccactttca cttcgggata caggcggctg aaagccggca tcagcggcgc aatgaaacgg
    16201 ctgccgaaag acagcggcac gttcatgcgc agcagacccg acgccttcac gcgctgcgag
    16261 gcgacggcag cttcggcctc gtcgatctcc ggcaggatac gctgacaggc ctccagataa
    16321 tcgctgcccg ccgccgtcag gctgagacgc cgtgtggtgc ggtggaacag cttgacgccc
    16381 agccgcgctt cgagcgcatt cacgtgcttg gcagccatcg ccggcgacat gcccaggtgg
    16441 cgcgcagcag ccgaaaggct gccgtccaat gcggcgcgca cgaagacccg catgccggtc
    16501 actcgatcca cgcgatttcc tactattggt atgaagtgtt ggtggatttt agccggttcc
    16561 gcccaatcgg taggcagttt agagtgaagc catcgaatca aggagcttca ccatgcgtca
    16621 cgacacccct actccggccc agacgcgcat gccggtgtat ttcgtgcccc acggcgcagg
    16681 accgtgcttt ttcatggact ggaatccggc ggacgcctgg cgccagatgg cggcctttct
    16741 caagaacatc gcggccacgc tgcccgcgcg cccgcgggcc gtgctggtga tttcagggca
    16801 ctggctgaca ccgcaatacg ccgtcaccgg ccacgcgcgg ccgtcactca tctacgacta
    16861 ctacggcttc ccgccgcaca cctatgaact cacctatccc gcacccggcg atccggcgct
    16921 ggcggcgcag gtggcggagc ggctggaaca aaacggattg gcctcccggg tggatccgga
    16981 tcgcggcttc gaccacggcg tgttcattcc gctgaagctg atgttcccca atgcggacat
    17041 tccgctggtg caggtctcgc tgcgccagga cctcgatccc gccgcgcacc tggcggccgg
    17101 ccgggcgctg gccgcgcttc gcgacgaagg cgtgctgatc gtgggcagcg gaatgagctt
    17161 ccacaacatg cgcggctacg gcgacccgcg ctacacgccg ctttcggccg agttcgacga
    17221 ctggctgacc gccgcggtga caagtcccgc cgaagaacgc gacacgcgcc tgcagcactg
    17281 gacagaggcg ccccacgccc accaatgcca cccggccggc cacgaggagc atctgattcc
    17341 gctgctggtt gccgccggcg ccgcgatgga gtcgatgggg cagcgcgtct acacggaaca
    17401 gatcatggaa accac
//
LOCUS       DATGOU010000139        14637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3993657, whole genome shotgun sequence.
ACCESSION   DATGOU010000139 DATGOU010000000
VERSION     DATGOU010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 14637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14637
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3993657"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..564
                     /locus_tag="VKZ48_08030"
     CDS             220..564
                     /locus_tag="VKZ48_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLU77833.1"
                     /translation="MKNSTAAVLLACALACAPAFAQQSGGAVKGDAERAQSKISMCVG
                     CHGIPEYRTAYPRVYRVPLIAGQAPEYIAIALRAYKSGDRNHPSMVGIAKTLSDQDIA
                     DLAAFYGRKSGE"
     gene            576..929
                     /locus_tag="VKZ48_08035"
     CDS             576..929
                     /locus_tag="VKZ48_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005665223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLU77834.1"
                     /translation="MTHRLLILALASLFATPVLAGDPAAGAEIAKKVCAACHGADGNS
                     SISPEFPKLAGQHEDYLLQALSQYKSGARKNPIMAAQVQPLSEADMRNLAAYFATQDG
                     SLYLIKETRLLRAAK"
     gene            complement(988..1410)
                     /locus_tag="VKZ48_08040"
     CDS             complement(988..1410)
                     /locus_tag="VKZ48_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003268866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HLU77835.1"
                     /translation="MFNPSREQVRQFFFDAWRKYRQGAQLEGLETIAVEAALMHPEYH
                     EVLDQPERYLDRDYTPEEGQSNPFLHMSMHVAIAEQLSIDQPPGIRAEFERLVNARGD
                     RHEALHEVLECLGETVWRAQRDRTPPDAEAYLECLRRR"
     gene            complement(1562..2209)
                     /gene="nth"
                     /locus_tag="VKZ48_08045"
     CDS             complement(1562..2209)
                     /gene="nth"
                     /locus_tag="VKZ48_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HLU77836.1"
                     /translation="MNAAKRAEIFARFQAANPEPTTELEYRTPFELLVAVILSAQATD
                     RSVNIATRKLFPVANTPQAIYDLGYEGLVPYIQTIGLYRTKAKNVLETCRLLLEKHGG
                     KVPDDRAALEALPGVGRKTANVILNTVFGHPTIAVDTHIFRVANRTGLAPGKNVVEVE
                     QKLLKCVPAQYRLHAHHWLILHGRYVCIARKPRCWECLIEDLCEYRAKTPAPGPA"
     gene            complement(2206..2829)
                     /locus_tag="VKZ48_08050"
     CDS             complement(2206..2829)
                     /locus_tag="VKZ48_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HLU77837.1"
                     /translation="MNPAQRVAEIDALLPQTQCTKCGYAGCEPYARAIAAGEADINRC
                     PPGGAAGIAALATLLDRPAKALDTTRGVERPHQVARIDESRCIGCTLCIQACPVDAIA
                     GAPKQMHSVIAALCSGCDLCVAPCPVDCIDMIPAANAWDRAAADAARNRFYRRRARLE
                     RDRAAREARRNRAVAAQDDEAQARQAFIRAAMERARARRAAAVPEGK"
     gene            complement(2826..3839)
                     /locus_tag="VKZ48_08055"
     CDS             complement(2826..3839)
                     /locus_tag="VKZ48_08055"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HLU77838.1"
                     /translation="MLYALARPFLFHLDAETAHELALCSADLLARLGGLTLPGRAIAG
                     RPVRAMGLDFPNPVGLAAGLDKNGAHIDGLATFGFGFIEVGTVTPRPQPGNPKPRLFR
                     IPEARAIVNRMGFNNEGVDALVANVRRARYDGILGINIGKNFDTPVERAADDYVACLE
                     KVYELASYVTINISSPNTKNLRQLQQSDELTALLERIARTRDGLKQRTQKQVPLAVKI
                     APDLDDDGIVAIADALRRYGIDAVIATNTTISREAVAGLPNADETGGLSGAPLTQRST
                     AVVRKLHEQLGSDIPIIGAGGIMSGADAADKLAAGASLVQLYSGLIYRGPDLVAEAVE
                     ATR"
     gene            complement(3968..4717)
                     /locus_tag="VKZ48_08060"
     CDS             complement(3968..4717)
                     /locus_tag="VKZ48_08060"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007588052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HLU77839.1"
                     /translation="MSKLNDLPQAVLQFYVTAPYPCSYLNGKLARSQVATPSHLIGND
                     LYSQLVRRGFRRSGIFTYRPYCDACRACVPVRIPVEEFAPTRYQRRAARRHESLQVNM
                     LNLRFRVEHYALYLRYQAARHAGGGMDQDSRDQYSHFLLQSRINTRLVEFRDEGRLAM
                     VSIVDYLEDGLSSVYTFFDPDATNASLGTYNILWQIEQCRALGLPHLYLGYWIRDSRK
                     MAYKARFRPIEGLIDGEWRRLDDDTIAQLQF"
     gene            complement(4724..5452)
                     /gene="aat"
                     /locus_tag="VKZ48_08065"
     CDS             complement(4724..5452)
                     /gene="aat"
                     /locus_tag="VKZ48_08065"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HLU77840.1"
                     /translation="MIAWLDRTLRFPPLTAALHEPNGLLAVGGDLAPQRLLEAYRRGI
                     FPWYSPGDPILWWSPDPRMVLFPDELKVSRSLTKTLRNKSYEVRFDTAFDAVIAACAA
                     PRNGETGTWIVPEIQEAYARLHRLGYAHSVETWIDGELAGGLYGVAIGRMFYGESMFT
                     AARDASKIALVHLVRLLAARGCGMIDCQMETPHLATLGARPIPRTEFARRLEELIHCG
                     HDTGSWQHFGPMHDGERIKARAAP"
     gene            complement(5457..5984)
                     /locus_tag="VKZ48_08070"
     CDS             complement(5457..5984)
                     /locus_tag="VKZ48_08070"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HLU77841.1"
                     /translation="MKFCSNCGDPVALRVPPGDNLPRYVCDACNTIHYVNPRMVVGCI
                     PEWEGRILLCRRAIEPRHGYWTLPAGFMENDETLAQAAMRETREEANARVALRDMYSV
                     LSVPRASQVHVFYRAELLDLDFGPGIESLEVRLFEEAEVPWDDLAFRTVSTTLRHYYT
                     DRRNGGFRLHTEDIG"
     gene            6161..7132
                     /locus_tag="VKZ48_08075"
     CDS             6161..7132
                     /locus_tag="VKZ48_08075"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HLU77842.1"
                     /translation="MLWGPPGVGKSQIIADIAARHDVPLIDIRLAQMEPTDLRGIPFR
                     DGRQVIWSVPAQLPDAERHGERGILFLDEITSAVPTVTAAAYQLILDRRLGEYSVPPG
                     WAIFAAGNRQGDRGVTYQMPAPLANRFAHYELEADLDDWVRWAHARGIDSRLIAFLRF
                     RPDLLFSFDPARYTVAFPSPRSWEYADRALHKFADAPALLGEALQACVGAAAGLACRA
                     YLNHLDALPDVDAIAEGRVHEVPRGVEMQYAVAAALVRHVARLSGAAQKSAVEHVLHY
                     ARRFPQRELGLMLVTDLQRTVATPLYEAPAFAEWAAEVGDLLSYERL"
     gene            7119..8354
                     /locus_tag="VKZ48_08080"
     CDS             7119..8354
                     /locus_tag="VKZ48_08080"
                     /inference="COORDINATES: protein motif:HMM:NF024601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA-like domain-containing protein"
                     /protein_id="HLU77843.1"
                     /translation="MSASERPQETASGRVKRKLEAARARLILDRPFIGELLMHLPLVE
                     HPRCGSIATDARALYYSRDYIDRLSFADVRFMLAHEALHCALLHFHRRGHRLERRWDR
                     ACDYCVNQLLVDDGFAPPEGALLDPAFRGLSAEEIYAVLPDAQDDHTLDEHWFGRAGS
                     GEAAIASDRGGDAEATQLADAAFMEAHRDGFDEVATRGSTLMPAEALAEAWQDRLAAA
                     AIEAAQVGSLGSAFDEALDRLIQPRLPWRALLARYLVSVARDDYSYGRPSRREGEALF
                     PALHSGQVELALALDTSGSITHHDFAEFLTEIDALKGQIRARVTLFACDAELAPDAPW
                     SFESWQPIALPEDLSGGGGTRFTPVFEWLEQRGTRPDALLYFTDAQGEFPAHAPGYPV
                     IWLVKGNAPVPWGERVQLN"
     gene            complement(8368..8874)
                     /locus_tag="VKZ48_08085"
     CDS             complement(8368..8874)
                     /locus_tag="VKZ48_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HLU77844.1"
                     /translation="MSANAAEIGISPQDAYAKVQQADAKVLFVDVRDPVEIMFVGFTD
                     AVHVNVPYLLVDRHAWDEKRGTFRLYRNPDFIARIKAELEKRGLGNDTEIITMCRSGS
                     ERGEPSARFLREHGFPNARYVIDGFQGSALKDGPHAGFRIRNGWQNSGLPWSTKMNPD
                     KMYHEAKR"
     gene            9071..9820
                     /locus_tag="VKZ48_08090"
     CDS             9071..9820
                     /locus_tag="VKZ48_08090"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006220627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HLU77845.1"
                     /translation="MSVDTLLPQIAEVLRNARAIAVLTGAGLSAESGIPTFRDAQTGL
                     WAQYRAEDLATPEAFERNPQLVWDWYEMRREGVRAAQPNPGHFALAAMQVQAPACTLI
                     TQNVDGLLQRAGGRMVLELHGNILRNTCFDDGCVVEAVLEGQRPPRCPQCGGMVRPDV
                     VWFGEELPADVLTHAIRAAKECDVFLSIGTSALVHPAAQLPMSALDNGATLIEVNRDP
                     TPLSPSAHYSVRALAGEFLPRLVKAAWPEAA"
     gene            9907..11382
                     /locus_tag="VKZ48_08095"
     CDS             9907..11382
                     /locus_tag="VKZ48_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HLU77846.1"
                     /translation="MGMPSYNDRRSMPAQVRSRWFYVLLVLAFILHALAALAQDAEQG
                     ATDIAPVVTLTIKGPIGVGAGMQVEQAVGLARERNASLLLITLDTPGGLVSTTREIIQ
                     SILGSSVPVAVYVAPSGARAASAGTYIVYAAHIAAMAPGTSIGAATPVAMQGPIGGPQ
                     PEEKPATRDKDGKDGKPAAPDTAMERKALNDAIAFLRSLAQMRGRNIEWADEAVRDAA
                     TLTGKEAASMNVVDLLADNQQALLEALHGRSVQTSAGKMEIVTRDAPLVAAEPSLRIR
                     VLAAVADPNVAFILLLIGIYGLIFEFWNPGTGVTGVIGAVCLLLALTALTMLPVHYGG
                     LALLLLGLALMTAEAFSPGVGVMGIGGLIAFVVGSIFLFDPSDSDFDFGVALPLILGA
                     AAASAGLFIFVLGAALRARRGPVATGAEQMIGARGVVREWQAQEGVVQVCGESWRARS
                     DQTLAPGVAVEVTARDGLVVEVRPLNNDSDFRGRAPLPDRP"
     gene            11397..12161
                     /locus_tag="VKZ48_08100"
     CDS             11397..12161
                     /locus_tag="VKZ48_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014971933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HLU77847.1"
                     /translation="MDLFALPFYLIPVLIVLGIIASAFRILREYERGVIFMLGRFYKV
                     KGPGLILVIPVLQQMVKVDLRVRVFDVPPQDVISRDNVSVKVNAVVYYRVVDPEHAVI
                     QVENFNQATSELAQTTLRSVLGQHELDEMLAEREKLNSDLRRLLDERTDAWGIKVSAV
                     ELKHVDLNEAMVRAIAKQAEAERLRRAKIIDAEGEQQAAEKLRQAGEVLATQPEAMQL
                     RYLGALTNIAGDRSSTIVLPLPLDLLNALSRAAERK"
     gene            complement(12164..12238)
                     /locus_tag="VKZ48_08105"
     tRNA            complement(12164..12238)
                     /locus_tag="VKZ48_08105"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12203..12205),aa:Arg,seq:cct)
     gene            complement(12292..13194)
                     /locus_tag="VKZ48_08110"
     CDS             complement(12292..13194)
                     /locus_tag="VKZ48_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77848.1"
                     /translation="MIISRRSSQLRSFLLYGGAHLFIVVVAAQADAREAWYVALPSMA
                     ALSLYGWIATFRRYRAVGDMPTSTIASAAQGYVELLGQAYNHPDWPLRAKLSQKLCCW
                     FAYEVEERESNGDWRTIDSGESVASFLLRDRTGECTIDPEGAEILCAHKETRDIATDR
                     RYTEWRIHEGDTLYVLGEFKTRTLLPGEADVRADMRELLAQWKRDPDCLLRRYDLDKN
                     GELDMKEWALARAAARREVERNHRVLRTVPSFDSIERPADGRLYLLSNHPPEKLARRF
                     VLWSWTHLGLFFAILAAFLLLFLG"
     gene            complement(13313..13879)
                     /locus_tag="VKZ48_08115"
     CDS             complement(13313..13879)
                     /locus_tag="VKZ48_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HLU77849.1"
                     /translation="MGLIIFLALIAVIVVYLIMVYNGLVNVKHGVAKAWSNIDVLLKQ
                     RHDEIPKLVEVCKQYRQFEQETLEKVIRARSLVQDAREKRDVDALGPAEAMMRSSLGT
                     LFAIAEAYPELKANEHFMQLQTRISQLENAIADRRELYNEAANINNVRIEQFPDLIVA
                     RLGSFPPAKLLEFATAEKADVDVKALFG"
     gene            14017..14595
                     /locus_tag="VKZ48_08120"
     CDS             14017..14595
                     /locus_tag="VKZ48_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006405586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HLU77850.1"
                     /translation="MQHDWQRVLDFWFGTADMPGYGQPRPEWFRKSDAFDETIRERFE
                     STYEAAARGELVHWQDVPESALALVIVLDQFPRNMYRGTPRAFAADPLALSVARHIVA
                     QGWDRSMKPVERLFVYLPYEHAEDMAMQRESVRLFETLRDDPQSVGGIDYAWRHYEII
                     ERFGRFPHRNAILGRPSTPEEIEFLKQPGSSF"
BASE COUNT         2451 a         4655 c         4906 g         2625 t
ORIGIN      
        1 tgcgtcaaca gattccggag acttcgcgtt gccggaccgc gtgcgcgctc gtgcccgtcg
       61 cgatcacttt tgaccggggc aagatcgctc ggttaaaatc tccccgcatt tgatctccat
      121 caaatgtggt ccggtcggcg acccggtcgc cagccaccca aacatgacag aaaaagcgcc
      181 tcgggcgcat catgcgggac ttacgagaga gcacccacaa tgaaaaatag cactgcagct
      241 gtgctgctcg cttgcgcctt ggcctgtgcg ccggcgttcg cgcagcagtc gggaggcgct
      301 gtcaaagggg atgcggagcg cgcccaaagc aagatctcca tgtgtgtcgg ttgccatggg
      361 attccggagt accgcacggc ctatccccgc gtttaccgtg tgccgctgat cgccggccag
      421 gcaccggaat acatcgccat cgccctgcgt gcttacaaga gcggcgaccg caaccatccc
      481 agcatggtgg gcatcgccaa gacgttgagc gaccaggaca ttgccgacct ggccgccttt
      541 tacggccgca agtccggcga gtgaggagga cgacaatgac ccatagacta ctcatcctcg
      601 cgctggcttc cctgttcgcc acgcccgtcc tggcgggcga ccccgcagca ggggcggaaa
      661 tcgccaagaa ggtttgcgcc gcctgccacg gcgcggacgg caacagttcc atctcgcccg
      721 agtttcccaa gctcgccggc cagcacgaag actatctgtt gcaggcgctc agccaataca
      781 agagcggtgc gcgcaagaac ccgatcatgg ccgcacaggt gcagccgctg agcgaggcgg
      841 atatgcgcaa tctcgccgcc tactttgcca ctcaggacgg ctcgctgtat ctcatcaagg
      901 agacgcgact gctgcgcgcg gcgaagtaac tcgcgatgca aaacaaaaaa ccccgctgcg
      961 gcggggtttt ttgtcggggc gacggactca gcgtcgccgc agacattcca gataggcctc
     1021 ggcgtccggc ggcgtgcggt cgcgctgcgc gcgccacacc gtttccccca ggcactccag
     1081 cacttcgtgc agcgcctcgt ggcggtcgcc gcgggcattg accagtcgct cgaactccgc
     1141 gcggatgccc ggcggctgat cgatggacaa ctgttcggcg atggccacat gcatgctcat
     1201 gtggaggaag ggattggact ggccttcctc gggcgtgtaa tcgcgatcga gatagcgctc
     1261 cggctggtcg agcacctcgt gatactccgg gtgcatcaac gccgcttcca cggcgatggt
     1321 ttccaggcct tccagttgtg cgccctggcg gtatttgcgc caggcgtcga aaaagaactg
     1381 gcggacctgt tcgcgactgg gattgaacat gacagagaga ttcctgcaaa agcgttacca
     1441 ccaagacacc aagacaccaa gaaaaatccg ttgaggaggc gctgctcggc tttcggtatg
     1501 gatggtcgag gacccggcaa cttggtgccc ttggtgcctt ggtggttcaa gactttaccg
     1561 gctaagccgg cccgggcgcc ggcgtcttgg cccgatactc gcacaaatcc tcgatcaggc
     1621 actcccagca ccggggtttg cgcgcgatgc agacatagcg cccgtgcagg atgagccagt
     1681 ggtgcgcgtg cagccggtat tgcgcgggga cgcacttcaa cagcttttgc tccacttcaa
     1741 cgacattctt gccgggcgcc aggccggtac ggttggcgac gcggaagatg tgtgtgtcca
     1801 ccgcaatggt cggatggccg aacacggtgt tgaggatgac gttggccgtc ttgcggccga
     1861 cgccgggcag ggcttccagc gcggcgcggt cgtccggcac tttgccgccg tgtttttcca
     1921 gcagcagacg gcaggtttcc agcacgttct tagccttggt gcggtacagg ccgatggtct
     1981 ggatgtacgg caccagtccc tcgtagccca ggtcgtagat cgcctgcggc gtgttggcga
     2041 cggggaagag cttgcgtgtg gcgatgttca cgctgcggtc ggtcgcctgt gcgctgagaa
     2101 tcacggcgac cagcagctcg aagggcgtgc ggtattccag ttcggtggtc ggttcgggat
     2161 tggccgcctg gaatcgcgcg aagatctcgg cccgcttggc ggcgttcatt tgccttcggg
     2221 tacggccgcg gcgcggcgcg cgcgcgcccg ttccattgcc gcgcggatga aggcctgtct
     2281 tgcctgcgct tcgtcgtcct gcgcggcgac ggcgcgattg cggcgcgctt cgcgtgcggc
     2341 cctgtcgcgt tccagtcgtg cgcgccggcg ataaaagcga ttgcgcgccg cgtcggcagc
     2401 ggcgcgatcc caggcgttcg ccgcggggat catgtcgatg caatcgacgg ggcagggcgc
     2461 cacgcacaga tcgcagccgc tgcacaacgc ggcgatcacc gagtgcatct gcttcggcgc
     2521 accggcgatg gcatcgaccg gacaggcctg gatgcacagc gtgcagccga tgcagcgcga
     2581 ttcgtcgatg cgcgccacct gatgcggccg ctcgacgccg cgcgtcgtat ccagcgcttt
     2641 ggccggccgg tcgagcaggg tggccagcgc ggcgatcccc gcggcgcctc cgggcgggca
     2701 tcggttgatg tcggcttcgc ccgcggcgat ggcgcgcgca tagggttcgc agccggcgta
     2761 gccgcatttg gtgcattgcg tctgcggcag cagcgcgtcg atttcggcga cccgttgcgc
     2821 gggattcatc gcgtcgcctc gaccgcttcg gccaccagat ccgggccgcg atagatcagg
     2881 ccgctgtaca gctgcaccag cgatgcgccg gcggcgagct tgtcggccgc atccgcaccg
     2941 ctcatgatgc cgcctgcgcc gatgatggga atgtcgctgc ccaattgttc gtgcagcttg
     3001 cgcaccacgg cggtcgagcg ctgcgtgagc ggcgcgcccg acaggccgcc ggtttcgtcg
     3061 gcgttcggca atccggcgac tgcttcgcgc gaaatggtgg tgttggtggc gatgaccgca
     3121 tcgatgccgt agcgccgcag cgcgtcggcg atcgcaacaa taccgtcgtc gtcgagatcg
     3181 ggcgcgatct tcaccgccag cggcacctgt ttttgcgtgc gctgcttgag gccgtcgcgc
     3241 gtgcgtgcga tgcgctccag cagtgccgtc agttcgtccg actgctgcaa ctgccggaga
     3301 ttcttggtgt tgggcgagga gatgttgatg gtgacgtagc tcgccagttc gtagaccttc
     3361 tccagacagg cgacgtagtc gtccgcggcg cgctccaccg gcgtatcgaa attcttgccg
     3421 atgttgatgc ccaggatgcc gtcgtaacgc gcgcgccgca cattggcgac gagcgcatcg
     3481 acgccttcgt tgttgaagcc catgcgattg acgatggcac gcgcctcggg aatgcggaac
     3541 aggcgcggct tggggttgcc cggctgcgga cgcggggtga cggtgcccac ttcgatgaag
     3601 ccgaagccga aagtcgccag accgtcgata tgcgcgccgt tcttgtccag acccgcggca
     3661 agccctacgg gattggggaa atcgagtccc atcgcgcgca ccggccgtcc ggcgatcgcg
     3721 cgaccgggca gcgtcaggcc gcccagtctg gcgagcagat cggcggagca cagagcgagt
     3781 tcgtgggcgg tttccgcgtc gaggtgaaac agaaacggcc gcgccagggc gtaaagcatg
     3841 ggccgcgagt ttagggaatg tccagggaag tgccaaggat ggctgtcggc acggccatca
     3901 cgatcggcga tgcttggatc atacaagctc gaacggcgct cgttcgtgag gcgcgtcgtg
     3961 ccggccgtca gaactgcagt tgggcgatcg tgtcgtcgtc caggcggcgc cactcgccgt
     4021 cgatcaatcc ttcgatgggg cggaagcgcg ccttgtacgc catcttgcga ctgtcgcgga
     4081 tccaatagcc cagataaaga tgcggcagcc ccagcgcgcg gcactgctcg atctgccaca
     4141 ggatgttgta ggtgccgaga ctggcgttcg tggcgtccgg atcgaagaaa gtgtagaccg
     4201 aggacaggcc gtcttcgagg taatcgacga tgctcaccat tgcaaggcgg ccttcgtcac
     4261 ggaattccac caagcgggtg ttgatgcggc tttgcagcag gaagtgcgag tactgatcgc
     4321 gactgtcctg atccatgccg ccaccggcat ggcgcgcggc ctggtagcgc agatacagcg
     4381 cgtagtgttc cacgcggaag cgcaggttca gcatgttcac ctgcagcgat tcgtggcgcc
     4441 gcgccgcgcg ccgctgataa cgcgtgggcg cgaactcctc gaccgggatg cgcaccggga
     4501 cacaggcgcg acaggcatcg caatagggac ggtaggtgaa gatgccgctg cggcggaagc
     4561 cgcggcgcac gagttggctg tacaggtcgt tgccgatgag atggctgggc gtggccacct
     4621 gcgagcgcgc cagcttgccg ttgaggtagc tgcacggata aggcgccgtc acgtagaact
     4681 gcagtacggc ctggggtaga tcgttaagct tcgacatcgg tcatcagggc gcggcgcgcg
     4741 cctttatgcg ctccccatcg tgcatcgggc cgaagtgctg ccacgagccc gtatcatgtc
     4801 cgcagtgtat caactcctcg aggcgccggg cgaattcggt gcgcggaatg ggccgcgcgc
     4861 ccagcgtggc gaggtggggc gtttccatct ggcagtcgat catgccgcaa cccctggcgg
     4921 cgagcagccg caccagatgc accagcgcga tcttggacgc gtcgcgtgcc gcggtgaaca
     4981 tcgactcgcc gtagaacatg cgtccgatgg ccacgccgta caggccgccg gccagttccc
     5041 cgtcgatcca ggtttcgacc gaatgcgcat agccgagccg gtgcagccgg gcataggctt
     5101 cctggatctc cggcacgatc caggtgccgg tttcgccgtt gcgcggcgcg gcgcaagcgg
     5161 cgatcaccgc gtcgaaggcg gtatcgaagc gcacttcgta tgatttgttg cgcaatgtct
     5221 tggtcaacga gcgcgaaacc ttcaactcgt ccggaaacag caccatgcgc ggatccgggc
     5281 tccaccacag gatcggatcg ccgggcgaat accaggggaa gatgccgcgc cggtaggctt
     5341 ccagcaggcg ctgcggcgcc aggtcgccgc ccacggcgag caggccgttc ggctcgtgca
     5401 gcgcggcggt gagcggcgga aagcgtagcg tgcggtccag ccaggcgatc atgcgatcag
     5461 ccgatatctt cggtgtgcag gcggaagccg ccgttgcggc gatcggtgta gtaatgccgc
     5521 aaggtggtgg aaacggtgcg gaaggcgaga tcgtcccacg gtacttccgc ttcctcgaac
     5581 agccttactt cgaggctttc gatgccggga ccgaaatcca ggtcgagcag ttccgcgcga
     5641 tagaacacgt gcacctggct tgcgcgcggc accgacagca cgctgtacat gtcgcgcaag
     5701 gccacgcgcg cgttcgcctc ctcgcgcgtt tcgcgcatgg cggcctgcgc gagggtctcg
     5761 tcgttttcca tgaagccggc gggcagggtc cagtagccgt gacgcggttc gatggcgcgg
     5821 cggcacagca ggatgcggcc ttcccattcg ggaatgcaac ccaccaccat gcgcggattc
     5881 acgtaatgga tggtgttgca ggcgtcgcaa acataacgcg gcaggttgtc gccgggcggc
     5941 acgcggagcg ccaccggatc gccgcagttg gagcagaact tcatcgcgga ccgggtgaga
     6001 caagggctcg aaactcgcgt gctatgatag cccacatccg acgccgcgct gcggcgctcc
     6061 catcaccact actgcgtccc gggtcttgcg tccttcgcat attgaggcgg tactagaacg
     6121 cgagttcgcc gctgttgcga gcgggcgaca cacgcccgtc atgctgtggg ggccgcccgg
     6181 tgtgggcaaa tcgcagatca tcgctgacat tgccgcgcgg catgatgtcc cgctgatcga
     6241 catccgcctt gcgcaaatgg agcccaccga tctgcgcggc atccccttcc gcgacggcag
     6301 gcaggtcatc tggtctgttc ccgcgcaact gcccgatgcc gaacggcacg gcgaacgcgg
     6361 catcctcttt ctcgatgaaa tcacctcggc cgtgccgacc gtcaccgccg cagcctatca
     6421 gctcattctc gatcgtcgcc tgggcgaata cagcgtgccg cccggttggg cgatcttcgc
     6481 cgccggcaac cgtcagggcg accgcggcgt cacctatcag atgcctgcgc cgttggcgaa
     6541 ccgcttcgcg cactacgaac tggaagccga tctggacgac tgggtgcgct gggcgcatgc
     6601 gcgcggcatc gactcgcgtc tgatcgcctt tctgcgcttc cgtcccgatc tgttgttcag
     6661 cttcgatccg gcgcgctaca ccgtcgcctt tccctcgccg cgatcttggg aatacgccga
     6721 ccgcgcactg cataaattcg ccgatgcgcc ggcgctgctc ggcgaagcgc tgcaggcctg
     6781 cgtcggcgcg gcggcgggac tggcctgccg cgcgtatctc aatcatcttg acgcactgcc
     6841 cgatgtggat gccatcgccg aaggccgcgt gcatgaggtg ccgcgcggcg tagagatgca
     6901 gtacgccgtg gccgcggcgc tggtgcggca cgtggcgcgc ctgtccggcg cggcacagaa
     6961 gtcggccgtc gaacatgtgc tgcactatgc gcgccgcttc ccgcagcgcg aactcggtct
     7021 gatgctggtg accgatctgc agcgcacggt ggccacgccg ctgtacgagg cgccggcttt
     7081 tgccgaatgg gcggccgagg tcggcgacct gctgtcctat gagcgcctct gaacggccgc
     7141 aggagacggc gtccgggcgc gtcaagcgca aactcgaagc ggcgcgtgcg cgcctcatcc
     7201 tcgaccggcc gttcatcggc gaactgctga tgcacctgcc gctggtggag catccgcgct
     7261 gcggcagcat cgccaccgac gcgcgcgcgc tgtactacag ccgcgattac atcgatcgcc
     7321 tgtccttcgc cgatgtgcgc ttcatgctgg cgcatgaggc gctgcactgc gcgctgctgc
     7381 attttcatcg gcgcggccat cgtctcgagc ggcgctggga ccgcgcctgc gactactgcg
     7441 tgaatcaact gctggtggac gacggcttcg cgccgcccga gggcgcgctg ctcgacccgg
     7501 cctttcgcgg tctgtccgcc gaggagatct acgccgtgct gcccgacgcg caggacgacc
     7561 acacgctgga tgaacattgg ttcggccgcg cgggcagcgg cgaggcggcc atcgccagcg
     7621 accgcggcgg cgatgccgag gcgacgcaat tggccgatgc ggcgttcatg gaggcgcatc
     7681 gcgacggctt cgacgaagtc gccacgcgcg gcagcacgct catgcccgcc gaagcgctcg
     7741 ccgaagcatg gcaggaccgc cttgccgccg ccgcgatcga ggcggcacag gtgggcagcc
     7801 tcggttcggc cttcgacgaa gcactcgatc gactgatcca gccgcgtctg ccgtggcgcg
     7861 ccctgctggc gcgctatctg gtgagcgtgg cgcgcgacga ctacagctac ggccggccga
     7921 gccggcgcga aggcgaagcg ctgtttccgg cgctgcacag cggccaggtg gaactggcgc
     7981 tcgcgctgga taccagcggc tccatcacgc accacgactt cgccgagttc ctcaccgaaa
     8041 tcgatgcgtt gaaggggcag atccgcgcgc gcgtcacgct gttcgcctgc gatgcggaac
     8101 tggcgccgga tgcaccctgg tccttcgaaa gctggcagcc catcgcactg ccggaagacc
     8161 tgagcggagg cggcggcacg cgtttcacgc ccgtattcga gtggctcgaa cagcgcggca
     8221 cgcgtcccga tgcgctgctt tatttcaccg atgcgcaggg cgaatttccc gcgcatgcgc
     8281 ccggctatcc ggtgatctgg ctggtgaaag gcaacgcgcc cgtcccctgg ggcgagcgcg
     8341 tgcagttgaa ctgacgaggc gcgcggttca gcgcttggcc tcgtgataca tcttgtccgg
     8401 attcattttc gtgctccacg gcaggcccga gttctgccag ccgttgcgga tgcggaatcc
     8461 ggcatgcggc ccgtccttca gcgccgaccc ctgaaagccg tcgatgacgt agcgtgcatt
     8521 ggggaagccg tgctcgcgca ggaacctcgc gctcggctcg ccccgttcgc tgcccgagcg
     8581 gcacatggtg atgatttcgg tgtcgttgcc caggccgcgc ttttccagtt cggccttgat
     8641 gcgcgcgatg aaatcgggat tgcggtaaag cctgaaggtg ccgcgctttt catcccaggc
     8701 atgccgatcg accagcaggt acggcacatt gacgtggacg gcgtcggtaa agccgacgaa
     8761 catgatttcc accgggtcac ggacgtcgac gaacaatact ttcgcgtcgg cttgttggac
     8821 tttggcatag gcatcctggg gggaaatccc gatctcggct gcgttggcgg acaatgcgca
     8881 gagcccgaat gagagtgcga tcaataagcg cttcatactc gtcctcgtgc ataaaatcgt
     8941 ggatgcgact agtgtagatg gcggcaggaa gacgggtttg cggtgcgtca agaaccgggc
     9001 acgccctgtc ggccggtcga agcgcagagg ctccgctatc atgcagtcgg caagtattgg
     9061 aggagacgcg atgtcagtcg atacgctgtt gccgcagatc gccgaggtgc tgcgcaatgc
     9121 ccgtgcgatc gcggtgctga ccggcgcggg tttgtccgcg gaaagcggca tcccgacttt
     9181 ccgcgacgcc cagacgggtt tgtgggcgca gtatcgcgcg gaggatctgg ccacccccga
     9241 agcctttgaa cgcaatccgc aactggtgtg ggactggtac gaaatgcggc gcgaaggcgt
     9301 acgcgcggcg cagcccaatc ccggccattt cgcgcttgcg gccatgcagg tgcaggcgcc
     9361 ggcgtgcacg ctcatcacac agaacgtcga cggcctgctg cagcgcgccg gcggacgcat
     9421 ggtgcttgaa ctgcacggca acatcctgcg caacacctgt ttcgacgacg gctgcgtggt
     9481 ggaggcggtg ctggagggcc aacgtccgcc ccgctgtccg caatgcggcg gcatggtgcg
     9541 gcccgatgtg gtgtggttcg gcgaggaact gcccgcggat gtgctgacgc atgcaatccg
     9601 ggcggcgaag gagtgcgacg tgttcctgtc catcggcacc tcggcactgg tgcatccggc
     9661 ggcacaattg cccatgagtg cgttggacaa cggtgcgacg ctgatcgagg tcaatcgcga
     9721 tcccacgccg ctttcgccgt cggcgcacta cagcgtgcgc gcgctggccg gcgagttcct
     9781 gcctcgtctg gtaaaagccg cctggccgga agcggcctga ccggcggctg cgcgccgcag
     9841 gctttgcttg tttcggcggc gcgcttcggc tagggtcgat gctgctcctt tacgggacgt
     9901 caccgcatgg gcatgccgtc gtacaacgat cgtcgctcga tgccggcgca ggtgcgcagc
     9961 cgttggttct acgtgctgct tgtgcttgcc ttcatcctgc acgcgcttgc cgccctggcg
    10021 caggatgcgg agcagggtgc gaccgacata gcaccggtcg tcacgctcac catcaaggga
    10081 ccgatcggcg tgggtgcggg catgcaagtg gagcaggcgg tcgggcttgc gcgcgagcgc
    10141 aacgcctcgc tcctgctcat cacgctcgat acaccgggcg ggttggtcag caccacgcgc
    10201 gaaatcatcc agtcgatcct cggctcgtcc gtgccggtgg ccgtttacgt agcgccttcg
    10261 ggcgcgcgcg cggccagcgc cgggacctac atcgtctatg cggcgcatat cgccgccatg
    10321 gcgcccggca ccagcatcgg tgcagccacg ccggtggcga tgcaggggcc cataggcggg
    10381 ccgcagccgg aagaaaagcc cgcgacccgg gacaaggacg gcaaggacgg caaacccgca
    10441 gcgcccgaca ccgcaatgga gcgcaaggcg ctcaacgacg ccatcgcctt tctgcgcagc
    10501 ctcgcgcaga tgcgtggccg caatatcgaa tgggcggatg aggcggtgcg cgacgcggcc
    10561 acgctcaccg gcaaggaagc ggcgagcatg aacgtggtgg atctgctcgc cgacaatcag
    10621 caggcgctgc tcgaggcgct gcacggccgc agcgtgcaga ccagcgcggg caagatggaa
    10681 atcgttacgc gcgacgcgcc gctcgtcgcc gccgaaccca gcctgcgcat ccgcgtgctg
    10741 gcggcggtgg ccgaccccaa cgttgctttc atcctgctgc tgatcggcat ctacggcttg
    10801 attttcgagt tctggaatcc gggaacgggc gtgacgggcg tgatcggcgc tgtgtgtctg
    10861 ctgctcgcgc tcaccgcatt gaccatgctg ccggtgcact acggcggact ggcgttgctg
    10921 ttgctcggtc tggcgctgat gacggccgag gccttcagtc ccggcgtggg cgtgatgggt
    10981 atcggcggcc tcatcgcctt tgtcgtcggc tcgatcttcc tgttcgatcc gagcgactcg
    11041 gacttcgact ttggtgtcgc actgccgctc atcctaggcg ccgctgccgc gagcgcgggt
    11101 ctgttcattt tcgtgctcgg ggcggcgctg cgcgcgcggc gcggaccggt tgccaccggc
    11161 gcggaacaga tgatcggcgc gcgcggcgtg gtgcgggagt ggcaggcgca ggaaggcgtg
    11221 gtgcaggtgt gcggcgagtc gtggcgggcg cgcagtgacc agacgcttgc gcccggcgtt
    11281 gcggtggaag tgacggcgcg cgacggcctg gtcgtcgaag tgcgcccgtt gaacaatgat
    11341 tccgatttcc gggggcgtgc gccgcttccc gacagaccct agcgaaggag ccgcatatgg
    11401 atttgttcgc cctgccgttc tacctgatcc cggtgctgat cgtgctgggg atcatcgcct
    11461 cggctttccg catcctgcgc gaatacgaac gcggcgtgat tttcatgctc ggccgcttct
    11521 acaaggtcaa gggaccgggt ctgatcctgg tcattcccgt gctgcagcag atggtgaagg
    11581 tcgatctgcg cgtgcgcgta ttcgatgtgc cgccgcagga cgtgatctcg cgcgacaacg
    11641 tctcggtgaa agtgaacgcc gtggtgtact accgtgtggt cgatcccgag catgcggtca
    11701 tccaggtgga gaatttcaac caggcgacca gcgaactcgc gcagacgacg ctgcgctccg
    11761 tgctcggtca gcacgaactg gacgaaatgc tcgccgaacg cgaaaagctc aatagcgatt
    11821 tgcgcagact gctcgacgag cgcaccgacg cctggggcat caaggtttcg gccgtcgaac
    11881 tgaagcacgt cgatctgaac gaggcgatgg tgcgggccat cgccaaacaa gcggaggccg
    11941 agcgcctgcg tcgcgcgaag atcatcgacg cggagggtga acagcaggcc gcggaaaagt
    12001 tgcggcaggc aggcgaggta ctcgcgaccc agccggaagc gatgcaactg cgctatctcg
    12061 gcgcgttgac caatatcgcc ggcgaccgct catcgaccat cgtgctgccg ctgccgctcg
    12121 atctgctcaa tgcgctgtca cgcgccgcgg agcggaagta gcgtggtctc gccgacagga
    12181 atcgaacctg tatctagcgc ttaggaggcg ctcgttctat ccgttgaact acggcgagaa
    12241 atgcgtttgc ggcgcagcgg tgcgcgcgat tttacgcgat gtggcgccga ttcacccgag
    12301 gaacagcagc agaaaggccg cgaggatcgc gaagaacagc cccagatgcg tccacgacca
    12361 taggacgaag cgtcgcgcga gtttctcggg cggatggttg gaaagcaggt agaggcggcc
    12421 gtcggcgggg cgctcgatgc tgtcgaagct gggcacggtg cgcagcaccc gatgattccg
    12481 ttccacctcg cgccgcgccg ctgcgcgcgc aagcgcccat tccttcatgt cgagttcgcc
    12541 gttcttgtcc agatcgtagc gacgcagcag gcaatcggga tcgcgtttcc attgggccag
    12601 cagttcgcgc atgtccgcgc gcacgtccgc ctcaccgggc aggagcgtac gggtcttgaa
    12661 ttcgcccagc acgtaaagcg tgtcgccttc gtggatgcgc cactcggtgt agcggcgatc
    12721 ggtggcgatg tcgcgcgtct ccttgtgcgc gcagagaatc tccgcgcctt cgggatcaat
    12781 ggtgcattcg ccggtgcgat cgcgcagcag gaaggaggcc acgctttcgc cagaatcgat
    12841 ggtgcgccag tcgccgttgc tttcgcgctc ttccacttcg taggcgaacc agcagcagag
    12901 cttttgcgac agcttggcgc gcagcggcca gtccggatgg ttgtacgcct gtcccagcaa
    12961 ttccacataa ccctgcgcag ccgaagcgat ggtcgaggtc ggcatgtcgc ccacggcgcg
    13021 gtaacgcctg aacgtggcga tccacccgta aagcgacagc gcggccatcg acggcagggc
    13081 gacgtaccac gcctcgcgcg catcggcctg cgcggcgacg accacgatga acagatgcgc
    13141 gccgccgtag agcagaaagc tgcgcaactg ggaactgcgt cgcgaaatga tcatgcgtgc
    13201 cgccgggttc cgccggcacg ccgttcaatg gcgttcatga gggaagcgcg cacggggaca
    13261 gccgtgcgtc gtcggggtca acaggggcgt cgatcgagcc tgctcgacat ccctagccga
    13321 acagcgcctt gacgtccacg tcggcctttt cggccgttgc gaattccagc agcttcgccg
    13381 gcgggaagct gcccaggcgc gcaacgatga gatcgggaaa ctgctcgatg cgtacattgt
    13441 tgatgttcgc cgcctcgttg tagagctcgc gtcggtcggc gatggcgttc tccagctggc
    13501 tgatgcgcgt ctgcaactgc atgaaatgtt cgttggcctt gagctcggga taggcttcgg
    13561 cgatggcgaa cagagtgccg aggctcgagc gcatcatcgc ttcggccgga cccagtgcat
    13621 ccacgtcgcg cttctcgcgc gcgtcctgca ccagcgagcg ggcgcggatc accttttcca
    13681 gcgtttcctg ctcgaactgg cggtattgct tgcagacctc caccagcttc ggaatctcat
    13741 cgtggcgctg cttgagcagc acgtcgatgt tcgaccaggc cttcgccacg ccgtgcttta
    13801 cgttgaccag gccgttgtag accatgatca ggtacaccac gatgacggcg atcagtgcaa
    13861 gaaagatgat cagccccact tgaatcctcc cgggaaggtg tctgcgcgcg gcattgtagc
    13921 cagtgcgcgg catgcgtcgg ctagaatcgc agtcttgagg cggagtgcct tgcgcgcttc
    13981 gtgaattttg ccgcgcatgc gcgacggagt gcagccatgc aacacgactg gcagcgagtg
    14041 ctggacttct ggttcggtac tgccgacatg ccgggatacg ggcagccgcg tcccgagtgg
    14101 ttccgcaagt cggatgcctt cgacgaaacg atacgcgagc gtttcgaaag cacctacgaa
    14161 gctgcagcgc gcggtgaact cgtgcattgg caggatgtgc ccgaatccgc gctcgcgctc
    14221 gtcatcgtgc tcgaccagtt cccgcgcaac atgtatcgcg gcacgccgcg cgccttcgcc
    14281 gccgatccgc tggccctgag cgttgcgcgt catatcgtcg cgcaaggctg ggaccggtcg
    14341 atgaaaccgg tagagcgcct gtttgtctac ctgccttacg agcacgccga ggacatggcg
    14401 atgcagcgcg aatcggtgcg gctgttcgaa accctgcgcg acgatccgca gagcgtcgga
    14461 ggtatcgact acgcctggcg ccattacgaa atcatcgagc gtttcggtcg cttcccgcat
    14521 cgcaacgcca tactcgggcg accgtcgacg cccgaggaaa tcgaatttct caaacaaccg
    14581 gggtcatctt tctaatgaat cacaccacca ccacgccgtc ggaatgggtc gcacgct
//
LOCUS       DATGOU010000140         3388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_4407274, whole genome shotgun sequence.
ACCESSION   DATGOU010000140 DATGOU010000000
VERSION     DATGOU010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4407274"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..644
                     /locus_tag="VKZ48_08125"
     CDS             60..644
                     /locus_tag="VKZ48_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HLU77851.1"
                     /translation="MPSRRLVLASTSPYRRALLERLRLPFEIAKPDVDESPLPGEAPA
                     ELALRLSEAKAREPAAAYPDSLLIGSDQVAVLDGALLSKPLTHDNAVRQLRAMRGRTV
                     EFLTGVAVHDTRTDRTLSRLVRYEVRLRNYTDDTIERYLRAEEPYDCAGSAKAEGLGI
                     VLIAGMAGDDPNSLIGLPLIALIDLLGEFDYPVL"
     gene            641..1348
                     /locus_tag="VKZ48_08130"
     CDS             641..1348
                     /locus_tag="VKZ48_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012400232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HLU77852.1"
                     /translation="MSGTLYLIPGPMGGDSLTDILPAGVIERLRQLDYFIAEHPKSAR
                     AFIKLSNPPRAIALLQIESIAADTPAAELDRLLDPVRDGKDAGLVSDAGAPAVADPGA
                     RLVARAHARGIRVAPLVGPSSILLALMASGLEGQRFAFHGYLPVDGAELATRVRELER
                     RAKAERQTQIFIETPYRNDRLLRVLLDACAPHTMLCLATHLTLPDESVRTATIAEWRK
                     APPQIGKRPTVFLLLSD"
     gene            complement(1345..2283)
                     /locus_tag="VKZ48_08135"
     CDS             complement(1345..2283)
                     /locus_tag="VKZ48_08135"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008249111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HLU77853.1"
                     /translation="MADDNNNWERSVLERLAFSALKEQRRARRWSIFFKLAGFAYLTV
                     LLVLAFDWTPTSEPGDGHTAMVEIDGVIDTAGNASAENIVDSLQAAFKDRGTKGVILR
                     INSPGGSPVQAGIIYDEIRRLRGEHPDIPIYAVVEDICASGGYYIAAAADRIYVDKAS
                     LIGSIGVVMSQFGFVETMDKVGVERRVLTAGENKAFLDPFSPLQPEHVEHAKVMLDEI
                     HQQFITAVKEGRGDRLKDAPGVYSGLIWTGARSLEIGLADALGSVQSVARDVIRAERV
                     SDFTQREGFAERFAKRLGRTMANVLQPLAPQSLSVR"
     gene            complement(2293..2643)
                     /locus_tag="VKZ48_08140"
     CDS             complement(2293..2643)
                     /locus_tag="VKZ48_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011298426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HLU77854.1"
                     /translation="MAAPARVICASEALVDGGAGIRFTVERNGRTLPAFVVRSEGVAH
                     AYINRCAHVGVELDWQEGQFFDPETGLLICATHGALYDPASGVCCEGPCRGGRLLPVS
                     IVERNGYIELKQDD"
     gene            complement(2622..3278)
                     /locus_tag="VKZ48_08145"
     CDS             complement(2622..3278)
                     /locus_tag="VKZ48_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004635673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HLU77855.1"
                     /translation="MPQQFDLLVFDWDGTLMDSAAAIVLSIQGACRDLGYPVPDEKRA
                     SHVIGLGLRDAMAHACPELRPSDYDRMIERYRYHWFRQDPDLPLFAGTVEMLAELEDR
                     GHLLAVATGKSRAGLARVLENTGLHRRFVSTRCADQCTPKPAPDMLLELIDELGADRE
                     RTLMIGDTTHDLNMAANAGVPAVAVSYGAHPPEELEALSPLAIVDSTPGLHQWLRRHA
                     "
BASE COUNT          580 a         1150 c         1068 g          590 t
ORIGIN      
        1 cgtcaaaaaa atcatagagg tagcgcacat ttgacgcgcc tcacgttgcg ccgcctccca
       61 tgccttctcg ccgcctcgtc ctagcctcca cttcgcccta ccggcgcgca ctgctggaac
      121 gcctgcgcct gcccttcgaa atcgccaagc cggacgtgga cgagagtccg ctgccggggg
      181 aagcgccggc cgaactggct ttgcgcctgt cggaagcgaa agcgcgcgag cccgcagccg
      241 cctatcccga cagcctgctg atcggttcgg atcaggtcgc cgttctcgat ggcgcgctgc
      301 tgtccaagcc gctcacgcac gacaatgcgg tgcggcagtt gcgcgccatg cgcggccgga
      361 cggtcgaatt cctgaccggc gtcgccgtcc acgacacacg caccgatcgc acgctcagcc
      421 gcctggtgcg ctatgaagtc cgtctgcgca attacacgga cgacaccatc gagcgctatc
      481 tgcgcgcgga ggagccctac gattgtgccg gcagcgcgaa ggccgagggc ctgggtatcg
      541 tactgattgc cggcatggcg ggcgacgatc cgaactcgct gatcgggctg ccgctcattg
      601 cgctgatcga tctgctcggc gaattcgact atccggtgct atgagcggca cgctctatct
      661 cattcccggc ccgatggggg gcgattcact gaccgacatc ctgcccgccg gcgtcatcga
      721 gcggctgcgc cagctcgact acttcattgc ggagcacccg aagagcgcgc gcgccttcat
      781 caagctcagc aatccgccgc gggcgatcgc cctgctgcaa atcgaaagca tcgctgccga
      841 tacgccggcg gcggaactgg accggctgct cgacccggtg cgggatggaa aggacgccgg
      901 gctggtgtcc gacgcgggcg cgccggccgt ggccgatccg ggtgcgcggc tggtggcgcg
      961 agcccacgcg cgcggcatcc gcgttgcgcc gctggtcggc ccctcctcca tcctgctcgc
     1021 gctgatggcc tcggggctcg aggggcagcg cttcgctttc cacggctatc tcccggtgga
     1081 cggcgcggaa ctggcgacgc gggtgcgcga actggagcgg cgcgccaaag ccgaacggca
     1141 gacgcagatc ttcatcgaga cgccctatcg caacgaccgg ctgttgcggg tactgctcga
     1201 tgcctgcgcg ccgcatacga tgctttgcct cgccacgcat ctgacgctgc ccgacgagtc
     1261 ggtgcgcacg gccacgatcg ccgaatggcg caaggcgccg ccgcagatcg gcaagcgccc
     1321 gacggtcttt ctattgctga gcgattagcg caccgacagc gactgcggcg cgagcggctg
     1381 caacacattc gccatggttc ggcccaggcg cttggcaaaa cgctcggcga aaccttcgcg
     1441 ctgcgtaaag tcgctcaccc gctccgcccg aatgacatcc cgggcaacgc tttgaacgct
     1501 gccgagcgca tcggccaagc cgatttccag gctgcgcgcg ccggtccaga tgagaccgct
     1561 gtacacgccg ggtgcatcct tcagccgatc gccgcgtccc tctttcaccg ccgtgatgaa
     1621 ctgttgatga atctcgtcga gcatcacttt ggcgtgctcg acatgctcgg gctgcaaggg
     1681 cgagaacggg tcgaggaaag ctttgttctc gccggcggtc agcacgcgcc gctcgacccc
     1741 gaccttgtcc atggtttcga cgaagccgaa ctggctcatc accacgccga tcgagccgat
     1801 gaggctcgct ttgtccacat agatgcgatc cgcggctgcg gcgatgtaat agccgcccga
     1861 ggcgcagata tcctccacca ccgcgtagat cggaatgtcc gggtgctcgc cgcgcaggcg
     1921 gcggatctcg tcgtaaatga tgcctgcctg caccggactg ccgccggggc tgttgatgcg
     1981 caggatcacg cccttggtac cgcggtcctt gaacgctgct tgcagtgagt cgacgatgtt
     2041 ctccgcactg gcattgcccg ccgtatcgat gacgccgtcg atttcgacca tcgcggtatg
     2101 tccgtcgccc ggctcgctcg tcggcgtcca gtcaaaagcc agaaccagca gcacggtcag
     2161 ataggcaaag ccggcgagtt tgaaaaagat gctccagcgc cgcgcacgcc gctgctcttt
     2221 caaggcgctg aacgcgagcc gctcgaggac gctgcgctcc cagttgttgt tgtcgtccgc
     2281 cattcgcttt cctcagtcgt cctgtttcaa ttcgatgtaa ccgttgcgct ccacgatcga
     2341 caccggcagc aaccggccgc cgcggcaggg accttcgcaa cagacgccgc tggccggatc
     2401 gtatagagcg ccatgggtgg cacagatcag caggccggtc tccggatcga agaactgccc
     2461 ttcctgccag tccagttcca ccccgacgtg tgcgcagcgg ttgatgtagg catgcgccac
     2521 gccctcgctg cggaccacga aagcaggcag cgtccggcca ttgcgttcga cggtgaagcg
     2581 gatacccgcg ccgccgtcca ccagcgcctc gctggcgcag attacgcgtg ccggcgcagc
     2641 cattggtgca accccggagt cgaatcgaca atggccagcg gcgacagcgc ttccagttcc
     2701 tccggcggat gggcgccgta gctgaccgcc acggccggta cgccggcatt cgccgccatg
     2761 ttcaggtcgt gcgtcgtgtc accgatcatc aaggtgcgtt cccggtcggc accgagttcg
     2821 tcgattaatt ccagcagcat gtcgggcgcg ggcttgggcg tgcactgatc ggcgcagcgc
     2881 gtggacacga aacgccggtg caggccggtg ttttccagca cgcgcgccag tcccgcgcgg
     2941 ctcttgcccg tcgcgacagc cagcagatgg ccgcggtcct ccagttcggc caacatttca
     3001 accgtgccgg caaacagcgg cagatcggga tcctggcgaa accagtggta gcggtagcgt
     3061 tcgatcattc tgtcgtagtc ggacgggcga agttccgggc aggcgtgtgc catggcgtcc
     3121 cgcaaaccga gtccgatgac gtggctggca cgcttctcgt ccggcaccgg gtagcccagg
     3181 tcgcggcagg cgccctggat cgacagcaca atggcggcag ccgagtccat caaggtgccg
     3241 tcccaatcaa aaaccaataa atcaaactgc tgcggcattt tcttcagagt tcaatgcaag
     3301 aaaagaggcg agttctgcag gcagcggcgc cgccaattcg agcggctcgc cactcagcgg
     3361 atgacgtagc ctgagttcgg ccgcgtgg
//
LOCUS       DATGOU010000141        11889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_6200577, whole genome shotgun sequence.
ACCESSION   DATGOU010000141 DATGOU010000000
VERSION     DATGOU010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11889
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6200577"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /gene="accD"
                     /locus_tag="VKZ48_08150"
     CDS             complement(<1..781)
                     /gene="accD"
                     /locus_tag="VKZ48_08150"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006399590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HLU77856.1"
                     /translation="MSWLQKLLPPKIQRNGAGGRRSVPEGLWHKCPECDSVLYTTDLE
                     NNASVCPKCGHHGRLSGRARLDLLLDPEGRFEIGAEVEPVDALKFKDSKRYPDRLRDA
                     MRHSGETDALVVMQGCVMQVPLVAAAFEFDFMGGSMGSVVGERFVRGVHACLEQNLPF
                     VCFTASGGARMQEGLLSLMQMTKTTAALTKLSERKLPFISVLTDPTMGGVSASFAFLG
                     DVVIAEPKALVGFAGPRVIEQTVREKLPEGFQRAEFLLKKGA"
     gene            complement(778..1578)
                     /gene="trpA"
                     /locus_tag="VKZ48_08155"
     CDS             complement(778..1578)
                     /gene="trpA"
                     /locus_tag="VKZ48_08155"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013205711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HLU77857.1"
                     /translation="MSRLTTIFEKLGREGRKALIPFITAGDPAGGDTVALMHALAEAG
                     SDVIELGIPFSDPMADGPVIQRASERALKQGVGLHTVLDYVRRFRQQDQQTGVVFMGY
                     ANPIEAMGAARFVEEATKAGIDGVLVVDYPPEECGEFAALAKQAGIDIIFLLAPTSTE
                     ARIARVAQLASGYIYYVSLKGVTGADTLDVDEVARKLAEIRKHTRLPLGVGFGIRDGE
                     TARAIGRAADAVVIGSRLIQTMEDAGEGAAVEQARRFVAAIREALDRP"
     gene            complement(1614..2813)
                     /gene="trpB"
                     /locus_tag="VKZ48_08160"
     CDS             complement(1614..2813)
                     /gene="trpB"
                     /locus_tag="VKZ48_08160"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HLU77858.1"
                     /translation="MQPYDLPDERGHFGPYGGSFVAETLVHALDELCAAYERYREDPE
                     FVAEFEYELKHFVGRPSPIYHAKRWSEQLGGAQIYLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKPRVIAETGAGQHGVATATVAARYGMECVVYMGSEDVKRQAANVYRMKLLGAT
                     VVPVESGSKTLKDALNEAMRDWVTNIHNTFYIIGTVAGPHPYPMMVRDFQSVIGEECL
                     TQMPEMIGRQPDAVIACVGGGSNAMGIFHPYIAHESVRLIGVEAAGYGLDSGKHAASL
                     TAGKPGVLHGNRTYLLQDENGQIIETHSVSAGLDYPGVGPEHAWLKDSARAEYVTITD
                     DEALKAFHDCCHYEGIIPALESSHALAYATKLAPTLARDKVLLVNLSGRGDKDMHTVA
                     EKSGIRF"
     gene            complement(2800..3435)
                     /locus_tag="VKZ48_08165"
     CDS             complement(2800..3435)
                     /locus_tag="VKZ48_08165"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HLU77859.1"
                     /translation="MHTRIKICGIREVAHAQAAARMGADAVGLVFYEPSPRHVSIERA
                     REIVAALPPFVAAVALFVNPSQAEVARVLEACPVPLLQFHGDETPDFCAGFGVPYLKA
                     ARVRPGMDLLEYLASYDSAAGWLLDAYRDDAYGGTGEAFDWNLIPGKLAKPLVLSGGL
                     TPDNVAEAVRRVRPWAVDVSSGVESAKGRKDEQLIAAFIAAVRAGMNHATV"
     gene            complement(3444..4229)
                     /gene="truA"
                     /locus_tag="VKZ48_08170"
     CDS             complement(3444..4229)
                     /gene="truA"
                     /locus_tag="VKZ48_08170"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012428635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HLU77860.1"
                     /translation="MRIALGLSYDGAPFAGWQSQPAGNTVQDRLEAALAAVADGPVRV
                     VAAGRTDAGVHAAAQVCHFDTMAERPLSAWVRGTNAHLPESVAVQWACEVDEDFHARF
                     SAVARTYVYVLYNHPVRPALHARQVGWFHRPLDVVAMQRAAKLLLGEHDFSAFRSAEC
                     QAAHPVRVMQAVNVSTHGPYVVFRFTANAFLHHMVRNLVGSLVYVGKGAQPPEWMREL
                     LAQRDRSAAAPTFSPAGLYLTEVRYPDTWRLPAFAPMILPLAV"
     gene            complement(4314..7016)
                     /locus_tag="VKZ48_08175"
     CDS             complement(4314..7016)
                     /locus_tag="VKZ48_08175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HLU77861.1"
                     /translation="MNTTTKLLASSLLLLSPVLVHAAGLGKLSVLSSLGQPLKVEIDV
                     VALQKGELASLNARVAAPEVFRRAGVEYGSVIPHLRAAIEPRNGGAVITLRSEQPVDE
                     PFVDVLVELDSASGNLVRQYTFLLDPVGYAGPTPIVAAPVTVAQPAPAEPAPAAKPIE
                     GESSRAPVAAAQAAPAAAEQPATATAPSQASSDQTPSQPATHEVKAGDTLSKIAMAYK
                     PADVSLQQMLVALQRANEDAFIAKNMNLVREGKILRIPDAESVQRIDQNEAVRVVNTH
                     TREFAEYRARIAAEVAAAEATGSASQQQASGRITTQVQEPPAPAEAPQDELRLSKSAE
                     GGGTTAGLASGDDAAATANALREANERVTQLERNLSDLQKLLELKNEQLAQLQQQAQT
                     ASVPADAPAETPAEPVAEAPAAPVTETEASTPAPAEAPSAEPVAETPAVEPAAAEATQ
                     PTADAATAIAKPEPEPEAEKPKRAKKKPVAQPAAMPEPSFLDTILDDPLLPAGALGAA
                     LALLLGLMIYRRRRAATLDNSLIGMTTTDSSSVFGTTGGRNIDTGASSLQTDFSQSAI
                     GSIDTEEVDPVAEADVYMAYGRDAQAEEILKEALQKDPSRLAVRLKLLEIYAARKDLK
                     AFETTATEIYAATGGQGADWEKVVTLGRSIDPNNSLYGAQQAAEAPPAAEPAAAAFAA
                     PAPEAAPAEPTAAAPAATTPEEAAPPMPMLDLDLNTDSAAAPAAPAVPDLPVEPSVSQ
                     ALDLDLDLNLGDTPAGPKTEEPMDFSPSGTFIMDAETKKAVSDLSESQSLDIDLGAPT
                     LEPKDARKPDLDAGLDFKLDEPAAETPKAEVPAVDLSGLSLDLDAGDAGDATYQEVAT
                     KLDLAKAYEEMGDKDGARELLNEVLKEGDAAQQEQARSMLETIG"
     gene            complement(7264..8412)
                     /gene="asd"
                     /locus_tag="VKZ48_08180"
     CDS             complement(7264..8412)
                     /gene="asd"
                     /locus_tag="VKZ48_08180"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013235241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HLU77862.1"
                     /translation="MKKVGLVGWRGMVGSVLMQRMEEERDFDLIDPVFFTTSNVGGQA
                     PTFGGKSSGALKDARSIDELKAMDIVITCQGGDYTNEVFPKLRAAGWKGYWIDAASAL
                     RMEDDAVIILDPVNQHVIDGALKQGVRNYIGGNCTNSILLMGVGSLFREGLVEWVSSM
                     TYQAASGGGANHMRELLKGMGVIHAGVADELANPASAILDIDRKVAQIIREDVPREYF
                     PAPLAGGLIPWIDKQVDGGQSREEWKGQAEVNKILGTSETVPVDGICVRIGAMRCHSL
                     ALTIKLKKDVPLEEIEHVIKSGNPWVKWVPNERSATERELTPASITGGLDIGVGRVRK
                     LNMGPEYVSAFVIGDQLLWGAAEPLRRMLRILLERDATQTAPAAARAA"
     gene            complement(8478..9533)
                     /gene="leuB"
                     /locus_tag="VKZ48_08185"
     CDS             complement(8478..9533)
                     /gene="leuB"
                     /locus_tag="VKZ48_08185"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HLU77863.1"
                     /translation="MKIAVLPGDGIGKEIVAQAMKVLRALDLGLTFEEAPVGGAGYEA
                     AGDPLPEATLKLAREADAILFGAIGDPRYDTLERAKRPEQSILRLRQALQLFANLRPA
                     TMHAELVSSSTLRPEIVSGLDLLIVRELTGDIYFGQPRGLRMVDGEREGYDTMHYRES
                     EIARIAHVGFKAARKRGKRLCSVDKANVLETSQLWREVVTGIGKEYPDVELSHMYVDN
                     AAMQLVRNPKQFDVIVTGNLFGDILSDEASMLGGSIGMLASASLDANGKGLYEPIHGS
                     APDIAGKDLANPLATILSAAMMLRYSLNQDAAADRVEAAVRKVLAQGLRTADIYEPGT
                     TKVGTGEMGDAVVAALN"
     gene            complement(9554..10195)
                     /gene="leuD"
                     /locus_tag="VKZ48_08190"
     CDS             complement(9554..10195)
                     /gene="leuD"
                     /locus_tag="VKZ48_08190"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011490490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HLU77864.1"
                     /translation="MEKFTVHRGLVAPLDRANVDTDAIIPKQFLKSIKRTGFGPNLFD
                     GWRYLDVGQPDSDNSKRPLNPDFVLNQPRYQGATILLARENFGCGSSREHAPWALVDY
                     GFRAVIAPSFAEIFYNNSFKNGLLPIVLSEAQVDELFNDTFAASGFELVIDLEGQRIA
                     YADGTKEIRFEIDPFRKYCLLNGLDDIGLTLRHADKIKAFEERRRAEQPWLFA"
     gene            complement(10206..10352)
                     /locus_tag="VKZ48_08195"
     CDS             complement(10206..10352)
                     /locus_tag="VKZ48_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HLU77865.1"
                     /translation="MLKQLKQVTLLGILVAFVAGCNTVEGFGRDVEKLGDKIERKAAQ
                     KRNY"
     gene            complement(10374..11780)
                     /gene="leuC"
                     /locus_tag="VKZ48_08200"
     CDS             complement(10374..11780)
                     /gene="leuC"
                     /locus_tag="VKZ48_08200"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HLU77866.1"
                     /translation="MSPQTLYDKLWASHAVHVEDDGTTLLYIDRQLLHEVTSPQAFEG
                     LRLASRKPWRISANLATVDHNVPTTDRSGGIDDPVARVQVQTLDQNCAEFGITQFGMN
                     DRRQGIVHVIGPEQGATLPGMTVVCGDSHTSTHGAFACLAHGIGTSEVEHVLATQCLL
                     AKKSKNMLVWFDGELPRGVTAKDMILAMIGEIGTAGGTGYAIEYGGPAVRALSMAGRM
                     TLCNMAIEAGARSGMVAADETTFEFLKGRPFAPRGEQWDRAVEYWRTLHSDEGAHFDR
                     VVNIDVTRIKPQVTWGTSPEMVTTVDGRVPDPAQESDPVKREGMERALHYMGLAPNTP
                     MTEIAIDKVFIGSCTNSRIEDLRAAAEVVRGRKLADNVKLAMVVPGSGLVKAQAEQEG
                     LDRIFKDAGFEWREPGCSMCLGMNADQLQPGERCASTSNRNFEGRQGAGGRTHLVSPE
                     MAAAAAVAGRFVDVRNWQ"
BASE COUNT         1959 a         3914 c         3815 g         2201 t
ORIGIN      
        1 tggcaccctt tttcagcagg aattcggcgc gctgaaagcc ttcggggagt ttttcgcgca
       61 cggtctgttc gatcacgcgc ggcccggcga agccgacgag cgccttgggc tcggcaatga
      121 ccacgtcgcc gaggaaggcg aagctggccg acacgccgcc catggtggga tcggtgagca
      181 cggaaatgaa cggcagcttg cgctcggaca gcttggtgag cgccgcggtc gtcttggtca
      241 tctgcatgag cgacagcagg ccttcctgca tgcgcgcgcc gcccgacgcg gtaaagcaga
      301 cgaaaggcag attctgttcg aggcaggcgt gcacgccgcg cacgaagcgc tcgcccacca
      361 ccgagcccat cgatccgccc atgaaatcga attcgaaagc ggcggcgacg agcggcacct
      421 gcatgacgca gccctgcatg accaccagcg cgtcggtctc gccgctgtgc cgcatggcgt
      481 cgcgcaggcg gtccggatag cgtttggaat ccttgaactt gagcgcgtcg accggctcga
      541 cttccgcgcc gatttcaaag cggccttcgg gatcgagcag cagatccagg cgcgctcgtc
      601 cggacaggcg gccgtgatgt ccgcacttcg ggcagacgga agcgttgttc tccagatcgg
      661 tggtgtacag caccgagtcg cattccggac atttgtgcca caacccttcc ggcaccgaac
      721 ggcgcccgcc ggcgccgttg cgctgtattt tcggcggcaa cagtttttgt agccaactca
      781 tggtcgatcc agtgcctctc tgattgcagc gacgaagcgg cgcgcctgtt ccaccgcggc
      841 gccttcgcct gcgtcctcca ttgtctgtat caagcggctg ccgatgacca ccgcatcggc
      901 ggcgcgtccg atcgcgcgcg ccgtctcgcc gtcgcgtatg ccgaagccca cgcccagggg
      961 cagacgagtg tgcttgcgaa tttccgcgag cttgcgcgca acctcgtcca catccagcgt
     1021 atccgcaccg gtcacgccct tgagcgagac gtaatagatg tagccgctgg ccagttgcgc
     1081 gactcgcgcg atgcgtgcct cggtggatgt cggtgcgagc agaaaaatga tgtcgatgcc
     1141 ggcctgcttc gccagtgccg cgaactcgcc gcattcctcc ggcgggtaat ccaccaccag
     1201 cacgccgtcg atgccggctt tggtcgcttc ctcgacgaaa cgcgccgcgc ccatcgcttc
     1261 gatggggttg gcataaccca tgaacaccac gccggtctgc tgatcctgtt ggcgaaagcg
     1321 gcgcacatag tcgagcacgg tatgcaggcc gacgccctgc ttgagtgcgc gttcgctggc
     1381 gcgctggatg accgggccgt cggccatcgg atcggaaaac gggataccca gttcgatgac
     1441 atccgagccc gcttcggcca gcgcatgcat cagcgccacg gtatcgccgc cggccgggtc
     1501 gccggccgta atgaacggga tcagcgcctt gcggccctcc cgccccagtt tttcgaaaat
     1561 tgttgtgagt ctcgacattc ggaaaacgcc gcgcggcgca gcgccttgga tggttagaag
     1621 cggatgcccg atttctccgc gacggtgtgc atgtccttgt cgccgcgccc cgagagattc
     1681 accagcagca ccttgtcgcg cgcaagcgtc ggcgcgagct tcgtcgcgta agccagcgca
     1741 tgactggatt cgagcgcggg aatgatgccc tcgtagtggc agcagtcgtg aaacgccttg
     1801 agcgcctcgt cgtcggtgat ggtcacgtat tcggcgcgcg cgctgtcttt cagccacgca
     1861 tgctcgggtc cgacgccggg atagtcgagc ccggcggaca ccgaatgcgt ttcgatgatc
     1921 tggccgttct cgtcctgcaa tagataggtg cggttaccgt gcagcacgcc cggcttgccc
     1981 gcggtgagcg aagcggcatg cttgccgctg tcgagcccgt accccgccgc ttcgacgccg
     2041 atgagacgca cgctttcatg tgcgatatag ggatggaaaa tgcccatggc gttggagccg
     2101 ccgccgacgc aggcgatcac cgcatcgggc tgccggccga tcatttccgg catctgcgtg
     2161 agacactcct cgccgatcac cgactggaaa tcgcgcacca tcatcgggta cggatgcggc
     2221 ccggcgacgg tgccgatgat gtagaaggtg ttgtgaatgt tagtgaccca gtcgcgcatc
     2281 gcttcattga gcgcatcctt gagtgtcttc gagccggact ccaccggcac gacagtcgca
     2341 ccgagcagct tcatgcggta gacgttggcg gcctggcgct tgacgtcttc ggagcccatg
     2401 tacaccacgc attccatgcc gtagcgcgcg gcgacggttg cggtcgccac accgtgctgg
     2461 ccggcgccgg tttcggcaat cacgcgcggc ttgcccatgc gcttggcgag cagcgcctgg
     2521 ccgatggtgt tgttcacctt gtgcgcgccg gtgtgattga ggtcttcgcg cttgagatag
     2581 atctgcgcgc cgcccagttg ttcggaccag cgcttggcgt gatagatggg gctcggccgg
     2641 ccgacgaagt gtttcagttc gtattcgaac tcggcgacga attccgggtc ctcgcgatag
     2701 cgctcatagg cggcgcacag ttcgtcgagc gcgtgcacca gggtttcggc gacgaaactg
     2761 ccgccataag ggccgaagtg gccccgctca tccggtaggt catacggttg catgattcat
     2821 ccctgcacgc actgccgcga taaacgcggc aatcaattgc tcgtccttcc gccccttggc
     2881 gctttccacg ccgctcgata catcgacggc ccagggacgg acgcggcgaa cggcttcggc
     2941 cacgttgtcc ggcgtcaatc cgccggagag gacgagcggc ttggcgagct ttccgggaat
     3001 cagattccag tcgaaagctt cccccgtccc gccgtacgca tcgtcgcgat aggcatccag
     3061 cagccacccg gctgccgagt cgtaggaagc caagtattct agcaaatcca tgcccggcct
     3121 tacccgcgcg gccttgaggt agggcacgcc gaaaccggcg caaaagtccg gcgtctcgtc
     3181 gccgtgaaac tgcagcagcg gcacggggca cgcttccagc acgcgtgcca cctccgcctg
     3241 cgacggattg acgaacaggg ccacagcggc aacgaagggc ggcagagcgg cgacgatctc
     3301 gcgcgcgcgc tcgatcgaga catggcgcgg gctcggctcg taaaacacca acccgacggc
     3361 atcggcgccc atgcgcgccg ccgcttgcgc gtgcgcgact tcgcgaatgc cgcaaatctt
     3421 gattcgggta tgcatggtca gggtcagacg gcgagaggca ggatcatagg cgcgaatgcc
     3481 ggcagacgcc atgtgtccgg gtagcgcacc tccgtcagat agagtccggc cggcgagaag
     3541 gtcggcgccg cggcgctgcg atcgcgctgc gcaagcagtt cacgcatcca ctcgggcggc
     3601 tgcgcgccct tgcccacata caccagcgag ccgacgaggt tgcgcaccat gtgatgcagg
     3661 aaggcgttgg cggtgaaccg gaagactacg tacgggccgt gtgtagatac gttcaccgcc
     3721 tgcatcacgc ggacgggatg tgccgcctgg cattcggccg aacgaaaggc gctgaagtcg
     3781 tgctcgccca gcagcagttt cgcggcgcgc tgcatcgcca cgacatccag cgggcggtgg
     3841 aaccagccaa cctgccgcgc atgcagggcc ggccgtacgg gatggttgta gagcacgtac
     3901 acgtaggtgc gcgcaacggc ggagaaacgc gcatggaaat cctcatcgac ctcgcaggcc
     3961 cactgcaccg cgacgctctc cggcaaatgc gcgttcgtgc cgcggaccca ggccgaaagc
     4021 ggccgttccg ccatcgtgtc gaagtgacag acttgcgcgg ccgcatgcac gccggcatcg
     4081 gtccgcccgg ccgccacgac gcgcacagga ccatcggcca cggccgccag cgcagcttcc
     4141 agccggtcct gtaccgtgtt tcccgcgggc tgcgattgcc agcccgcaaa cggcgcgccg
     4201 tcgtagctca gacccagtgc gattctcaat ggatggatga acccggaaaa aagaaggccg
     4261 cacaagcggc cttccgatct taggaaatcc ccgaagcatc ggggaagcgg cgatcagccg
     4321 atcgtttcga gcatactgcg cgcctgctcc tgctgggcag cgtccccttc cttgaggact
     4381 tcgttcagca gttcgcgtgc gccgtccttg tcgcccatct cctcgtaagc cttggccagg
     4441 tcgagtttgg tggcgacctc ctggtaggtg gcgtcgcccg catcgccggc gtccaggtcg
     4501 aggctcaggc cgctcaaatc gacggcgggc acctcggcct tgggtgtttc ggcagccggt
     4561 tcgtccagct tgaagtccag tcctgcatcc aggtcgggct tgcgcgcatc cttcggctcg
     4621 agagtcggtg cgcccagatc gatgtccagg gactgcgatt cgctcagatc gctcacggcc
     4681 ttcttcgtct cggcgtccat gatgaaggta ccgctgggcg aaaagtccat cggctcctcc
     4741 gtcttggggc cggccggcgt atcgcccagg ttcaggtcca gatcgagatc cagggcctgc
     4801 gaaacactgg gctcgaccgg cagatcgggc acagccggcg cggcgggtgc agccgcggaa
     4861 tcggtgttca gatcgagatc gagcatcggc atgggcggcg cggcttcctc gggcgtcgtt
     4921 gcagccggcg ccgccgcggt gggctcggcc ggcgcggctt cgggcgcggg cgctgcgaac
     4981 gctgccgcag caggctcggc tgccggcgga gcctcggccg cctgctgcgc gccatacaaa
     5041 ctgttgttgg gatcgatgga gcgacccagg gtcacgacct tctcccaatc ggcgccctgc
     5101 ccgccggtgg cagcgtagat ttccgtggcc gtcgtttcga aggccttgag atctttgcgc
     5161 gcggcataga tttccagcag cttgagccgg acggccagac ggctggggtc cttctgcagc
     5221 gcttccttga ggatttcctc ggcttgcgca tcgcggccgt aagccatgta cacgtcggct
     5281 tcggcgacag gatcgacttc ctcggtatcg atggagccga ttgcgctctg gctgaagtcg
     5341 gtctgcagcg aactggcgcc ggtatcgatg ttgcgaccgc cggtggtgcc gaacaccgaa
     5401 ctggaatcgg tggtcgtcat gccgatcagg ctgttgtcga gcgtggcggc gcgacggcga
     5461 cgatagatca tcaggccgag caacagggcc agcgccgcac ccagcgcgcc ggcaggaagc
     5521 agcggatcgt ccaggatcgt atcgaggaaa ctcggctccg gcatcgccgc aggctgcgcg
     5581 acgggctttt tcttcgcccg cttgggcttc tcggcctcgg gctcgggttc gggctttgcg
     5641 atggccgtcg cagcgtccgc agtcggctgg gtcgcctcgg cggccgccgg ctcgacggcg
     5701 ggcgtttcgg caaccggttc ggcgctgggc gcttcggcag gcgccggcgt cgacgcctcg
     5761 gtctcggtca ccggggcagc aggcgcctcg gcaaccggtt cggcaggggt ttcggccggg
     5821 gcgtcggccg ggacggacgc cgtctgggcc tgttgctgca attgcgccag ttgctcgttc
     5881 ttcagttcca gcagcttctg caaatcgctc aggttgcgtt ccagctgggt gacgcgctca
     5941 ttcgcttcgc gcaaagcatt tgccgtcgct gccgcatcat cgccgcttgc aagaccggca
     6001 gtggtcccgc cgccttcggc gctcttggac aggcgcagtt catcctgcgg cgcttccgcc
     6061 ggcgcgggcg gctcctgaac ttgcgtggtg atgcggccgg acgcctgctg ctggctggcg
     6121 gagccggtcg cttccgccgc agcaacttcc gccgcgatcc gcgcgcggta ttcggcaaat
     6181 tcgcgcgtat gcgtgttcac cacgcggacc gcctcgttct ggtcgatccg ctgaacgctt
     6241 tccgcgtccg ggatgcgcag gatcttgccc tcgcgcacca ggttcatatt cttggcgata
     6301 aaggcgtctt cgttggctcg ctgcagcgcc accagcatct gctggaggct cacatccgca
     6361 ggcttgtacg ccatggcgat tttcgacagc gtatcgccgg ctttgacttc atgggtcgca
     6421 ggctgcgagg gggtctggtc cgaggacgcc tgcgacggcg ccgtagcggt cgccggctgc
     6481 tcggcagcgg caggcgcggc ctgagcggcc gccaccgggg cgcgcgacga ctcgccttcg
     6541 atcggctttg cagccggtgc cggctcggcg ggcgcgggtt gcgccactgt gacgggcgcg
     6601 gcgacgatcg gcgtcggtcc cgcgtagccg accgggtcga gcaggaaggt gtactgacga
     6661 accagattgc cgctggcgga atcgagttcc accagcacgt cgacgaacgg ctcgtcgacc
     6721 ggctgttcgc tgcgcagcgt gatcaccgca ccgccgttgc ggggttcgat ggcggcgcgc
     6781 agatggggaa tgacgctgcc gtactcgaca cccgcccggc ggaacacctc gggagcggcg
     6841 acgcgcgcat tgagcgaggc aagttcgcct ttctgcaagg cgaccacatc gatttcgacc
     6901 ttgagtggct gaccgagcga agacagcaca ctgagcttgc ccagaccggc cgcgtgcaca
     6961 agcaccggcg acagcagcag gagcgaactg gcaagcagtt tagttgttgt attcaaggga
     7021 tttcccactg caaatccttg ggatgtggac cagaggcaac gaaaatagca tcaggggttt
     7081 agccccgcaa gctaagccaa cggcttaaat ccgcctttag ccaaaaagtt ctcgaaaggc
     7141 cgggcccctc gcacacgcca taggaaatat gtcgctgtca tgactcttcg ggacatggcg
     7201 cgccgggcgt ccgacaaaaa agaagggaag cccgcaggct tccctcgcgt tactccaaac
     7261 gaatcaggcg gcgcgtgctg cggccggggc ggtttgcgtc gcgtcgcgct cgagcaggat
     7321 gcgcagcatg cggcgcagcg gttcggccgc accccacagc aactgatcgc cgatgacgaa
     7381 ggcggagacg tactcgggcc ccatgttgag cttgcgcaca cgccccacgc cgatgtcgag
     7441 gccgccggta atggatgcgg gcgtcagttc tcgctccgtg gccgagcgct cgttcggcac
     7501 ccatttcacc cacgggttgc cggacttgat gacgtgctcg atttcctcga gcggcacatc
     7561 cttcttcagc ttgatcgtca gcgccaggct gtggcagcgc atcgcgccga tgcggacgca
     7621 aatgccgtcg acgggaacgg tctcgctggt gccgaggatc ttgttgacct cggcctgccc
     7681 cttccattcc tccctggatt ggccgccgtc cacctgcttg tcgatccagg gaatgaggcc
     7741 gcccgccagc ggcgcgggga agtactcgcg cggcacatcc tcacgaatga tctgcgcgac
     7801 tttgcggtcg atgtcgagaa tcgccgatgc gggattggcc agctcgtcgg ccacgccggc
     7861 atggatgacg cccatgccct tgagcagttc acgcatgtgg ttcgcgccgc cgcccgaagc
     7921 cgcctgatag gtcatggagc tcacccattc caccagtcct tcgcggaaca ggctgcccac
     7981 ccccatcagc aggatggagt tggtgcagtt gccgccgatg tagttgcgca cgccctgctt
     8041 gagcgcaccg tcgatgacgt gctggttgac cgggtcgaga ataatgacgg cgtcgtcttc
     8101 catgcgcagg gcggaagcgg cgtcgatcca gtagcccttc cacccggccg cgcgcagctt
     8161 ggggaagacc tcgttggtgt agtcgccgcc ctggcaggtg atgacgatat ccatcgcctt
     8221 cagctcgtcg atgctgcgcg catccttcag cgccccgctc gacttgccgc cgaaggtcgg
     8281 cgcctggccg cccacattgg aagtcgtgaa gaacaccgga tcgatcagat cgaaatcgcg
     8341 ttcttcttcc atgcgctgca tgagcacaga accgaccatg ccgcgccaac ccaccaaccc
     8401 tacctttttc atcaccgctg tcccataagt ttcttgcttc agtggcgcgg catcgtccgc
     8461 gcccgccctg ccccgcttca attcagggcg gccaccaccg cgtcgcccat ttctccggtg
     8521 ccgaccttgg tcgtgcccgg ctcataaatg tccgccgtcc gcagcccttg tgcaagcact
     8581 ttgcgcacgg ccgcctcgac cctgtccgcc gccgcatcct gattaaggct gtagcgcagc
     8641 atcatcgccg ccgaaagaat ggtcgccagc ggattggcga ggtccttgcc cgcgatgtcc
     8701 ggcgccgacc cgtggatcgg ctcgtacaga cccttgccgt tcgcgtcgag cgaggcggag
     8761 gccagcatgc cgatggagcc gccgagcatc gaggcttcgt cggacaggat gtcgccgaac
     8821 aggttgcccg tcacaatgac gtcgaactgc ttcggattgc gcaccaactg catcgcagcg
     8881 ttatccacgt acatgtgcga caactcgaca tccggatatt ccttgccgat ccccgtaacg
     8941 acctcgcgcc acagttgcga ggtttccagc acgttggcct tgtcgaccga gcacagccgc
     9001 ttgccgcgct tgcgcgccgc cttgaagccc acatgtgcga tgcgtgcaat ttcggactcg
     9061 cgatagtgca tggtgtcgta gccttcgcgt tccccatcga ccatgcggag gccgcgcggc
     9121 tggccgaaat agatgtcgcc ggtgagttcg cgcacgatca gcagatcgag gccggacacg
     9181 atttccggcc gcagggtgga actgcttacc agttccgcgt gcatggtcgc cgggcgcagg
     9241 ttggcgaaca actgcagcgc ctgacggagg cgcaggatcg attgctcagg ccgcttcgcc
     9301 cgctccagcg tgtcgtagcg cggatcgccg atggcgccga acaggatggc atcggcttcc
     9361 cgggcgagct tgagggtcgc ttccggcagc ggatcgcccg ccgcttcgta acctgcgccg
     9421 ccgaccggcg cttcctcgaa cgtcagtccg aggtcgagcg cgcgcaacac tttcatcgcc
     9481 tgcgcgacga tttccttgcc gatcccgtcc ccgggcaata ccgcgatttt catagttgat
     9541 tcccgtctac tcttcaggcg aacagccagg gctgttccgc ccggcggcgt tcttcgaaag
     9601 ccttgatctt gtcggcatga cgcagcgtca ggccgatgtc gtccagtccg ttcagcaggc
     9661 aatacttgcg gaaggggtcg atctcaaagc gtatttcctt cgtgccgtcc gcataggcga
     9721 tgcgctgccc ttccaggtcg atcaccagtt cgaagcccga ggcagcgaag gtatcgttga
     9781 acagctcgtc gacctgcgcc tcgctcagca cgatgggcag cagtccgttc ttgaaactgt
     9841 tgttgtagaa gatttccgcg aaggacggcg cgatcacggc gcgaaagccg tagtccacca
     9901 gcgcccaggg cgcgtgctcg cgcgaactgc cgcagccgaa gttctcgcgc gcgagcagga
     9961 tcgtggcgcc ctgatagcgc ggctggttca gcacgaaatc cggattgagc gggcgcttgc
    10021 tattgtcgct gtccggctga ccgacatcga gatagcgcca gccgtcgaac aggttcggcc
    10081 cgaagcccgt gcgcttgatg gatttgagga actgcttcgg gatgatcgcg tcggtatcga
    10141 cgttcgcccg atcgagcggg gcgaccagtc cgcgatgcac ggtgaatttt tccattgctg
    10201 cgttctcagt aattgcgctt ctgtgcggct ttgcgttcga tcttgtcgcc cagcttttcg
    10261 acatcgcgac caaagccttc cacggtgttg cagcccgcca cgaaggcgac gaggatgcca
    10321 agcagcgtca cttgtttgag ttgtttgagc atcgcgtact ccagaacctc tacctattgc
    10381 cagttacgca catcgacgaa acggcccgcg accgccgcgg cggccgccat ttcaggactc
    10441 accagatgcg tgcgcccgcc cgcaccctgc cggccttcga agttccggtt ggaggtcgag
    10501 gcacagcgct cgcccggctg caattgatcg gcattcatgc ccagacacat cgagcagccc
    10561 ggctcgcgcc attcgaagcc cgcgtccttg aaaatgcggt ccaggccttc ctgctcggcc
    10621 tgtgccttca ccagacccga gccggggacc accatggcga gcttgacgtt gtccgccagt
    10681 ttgcggccgc gcacgacctc ggcggcggcg cgcagatcct cgatacgcga gttggtacag
    10741 gaaccgatga acaccttgtc gatcgcgatc tcggtcatgg gcgtgttggg cgcaaggccc
    10801 atgtagtgca gcgcgcgctc catgccttcg cgcttgaccg ggtcgctctc ctgcgccgga
    10861 tcgggcacgc ggccgtcgac ggtggtgacc atctccggcg aggtgcccca ggtgacctgc
    10921 ggcttgatcc gggtgacatc gatgttcacc acgcgatcga agtgcgcgcc ctcgtcgctg
    10981 tgcaaagtgc gccaatactc gaccgcgcga tcccattgct cgccgcgcgg cgcgaagggg
    11041 cggcccttga gaaattcgaa ggtggtttcg tcggcggcca ccatgccgct gcgcgcgccg
    11101 gcctcgatcg ccatgttgca aagcgtcatc cgtccggcca tggacagcgc gcgcacggcc
    11161 ggaccgccgt actcgatcgc atagcccgtg ccgcccgcgg tgccgatctc gccgatcatg
    11221 gcaaggatca tgtccttggc cgtcacgccg cgcggcaatt cgccgtcgaa ccagaccagc
    11281 atgttcttcg acttctttgc cagcaggcat tgcgtggcca gcacgtgctc gacttcggag
    11341 gtgccgatgc cgtgcgccag gcaggcaaac gcaccgtgtg tgctggtgtg gctgtcgcca
    11401 cagacgaccg tcatgccggg cagcgtcgcg ccctgctccg ggccgatgac atgcacgatg
    11461 ccctggcgcc gatcgttcat gccgaattgg gtgatgccga actcggcgca gttctggtcc
    11521 agggtctgca cctgtacgcg cgccaccgga tcgtcgatac cacccgagcg gtcggtggtc
    11581 ggtacgttgt gatcgacggt ggccagattg gcgctgatgc gccagggctt tcggctggcc
    11641 agccgcagcc cttcgaaggc ctgcgggctg gtcacctcat gcagcagctg gcgatcgata
    11701 tagagcagcg tcgtgccgtc gtcctcgaca tgcacggcat ggctggccca gagtttgtcg
    11761 tacagcgttt gggggctcat cgggagcgga agcaaaaaac agggtcggaa attatacccc
    11821 ggataccccg ctattccgct tcggtccgcg ctcgcggccc atggcaaacc agcccgctac
    11881 ttcctcgcc
//
LOCUS       DATGOU010000142         7785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3107875, whole genome shotgun sequence.
ACCESSION   DATGOU010000142 DATGOU010000000
VERSION     DATGOU010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 7785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7785
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3107875"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..393)
                     /gene="iscA"
                     /locus_tag="VKZ48_08205"
     CDS             complement(70..393)
                     /gene="iscA"
                     /locus_tag="VKZ48_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HLU77867.1"
                     /translation="MAITLTDRAATHVQDFLAKRGKGIGLRVGVQTSGCSGMAYKLEF
                     ADEVQPDDHTFESNGIKVIVDPKSLPYIDGTELDFAREGLNEGFKFNNPNVKDSCGCG
                     ESFNI"
     gene            complement(460..930)
                     /gene="iscR"
                     /locus_tag="VKZ48_08210"
     CDS             complement(460..930)
                     /gene="iscR"
                     /locus_tag="VKZ48_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HLU77868.1"
                     /translation="MRLTTKGRFAVTAMLDLALNSGSGPVPLAAISQRQSISLSYLEQ
                     LFGKLRRHKIVESVRGPGGGYYLARPAEHVSVADIIIAVDEPLDATQCGGKENCHKDT
                     RCMTHDLWAQLNVRMYDYLESVKLADLVAQQNGTAPAPLVMHSHTEKTQTLLSA"
     gene            complement(1036..1785)
                     /gene="cysE"
                     /locus_tag="VKZ48_08215"
     CDS             complement(1036..1785)
                     /gene="cysE"
                     /locus_tag="VKZ48_08215"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HLU77869.1"
                     /translation="MFARIREDIASVFDRDPAARNTWEVLTCYPGFHALLSHRLAHKL
                     WKAKFRWLARLLSHVARWFTGIEIHPGAVIGRRVFIDHGMGVVIGETAEIGDDTTLYH
                     GVTLGGTSWNKGKRHPTLGKNVVVGAGAKILGPILVGDGAKIGSNAVVVKDVPAGATA
                     VGIPARIIEDQHEQAREAHAERLGFSAYGLSKEVDDPVAQAIRGLTDHTAELERRLEA
                     LTRELEQLRAQEDAQAIGAGFDPVLRKVADK"
     gene            complement(1845..2576)
                     /locus_tag="VKZ48_08220"
     CDS             complement(1845..2576)
                     /locus_tag="VKZ48_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017234697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HLU77870.1"
                     /translation="MSAALERVRIVLSHTTHPGNIGAAARAMKTMGLSRLVLINPKRF
                     PDPQAEAMASGAADVLANARVCASLDEALAGTRFAVALTARRRDLSHIAADMRESARM
                     LVEEAGQGEVALVFGTEMSGLSNEDVLKCQRIAHIPANPEYSSLNLAAAVQVGAYEVR
                     MAALGAPSIDNERFDAAAYEDIEGFYAHLERSLTESGFLDPAHPKRLMERLRRLYGRV
                     RLEKEEVNILRGILTSWEQNHRRKS"
     gene            2675..3505
                     /locus_tag="VKZ48_08225"
     CDS             2675..3505
                     /locus_tag="VKZ48_08225"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006051471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HLU77871.1"
                     /translation="MHALHPMLNTAIKAARRAGAVINRASRDLDVLSVTAKRRNDFVT
                     EVDRAAEEAVIEVLRTAYPDHAILAEESGASGTSEYEWIIDPLDGTTNFIHGFPQYAV
                     SIALRQKGVMQQAVIYDPTRNELFTATRGRGAYLNDQRIRVSKRAQLKDSLIGTGFPF
                     REVDDIDTYLRIFKRVTFETAGIRRAGAAALDLAYVACGRLDAFWEKGLAPWDMAAGS
                     LIVLEAGGLISDYDGEADFMESGEVVCGNPKVFAQLLQLVQKQRRARTPAAGTPAAEE
                     "
     gene            complement(3731..4252)
                     /locus_tag="VKZ48_08230"
     CDS             complement(3731..4252)
                     /locus_tag="VKZ48_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HLU77872.1"
                     /translation="MEFLNTIKARIPDYAKDVRLNLDGTIARSSLEKTDALGAALAAA
                     YATGNKFMVDTIRNSGELSDVDVNAALTAAALMGMNNVWYPYVEMSGDEELSKQRAEL
                     RMNAYATHGGVDRNRFELFALAASIIGKCHFCIGSHYKLLKEAGMTVQQLRDVGRIAA
                     SVAAAAQVLAVQD"
     gene            complement(4348..4896)
                     /locus_tag="VKZ48_08235"
     CDS             complement(4348..4896)
                     /locus_tag="VKZ48_08235"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004192241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HLU77873.1"
                     /translation="MKTVGDKLEAFSVVGVKPGFNQHEEKGQSAFEEINEKSFPGKWK
                     IIYFYPKDFTFVCPTEIVGFGKLNAQFQERDAVLMAGSTDNEFSKLGWRREHPDLKGL
                     NHYQFADTTGSLVDQLGIREKNEGVALRATFIVDPHNVIQHVSVNNLNVGRSPEEVLR
                     ILDGLQTDELCPCNRAVGAETL"
     gene            5199..7763
                     /gene="mutS"
                     /locus_tag="VKZ48_08240"
     CDS             5199..7763
                     /gene="mutS"
                     /locus_tag="VKZ48_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006408198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HLU77874.1"
                     /translation="MSSSTPAAHTPMMQQYLGLKAQYPDLLLFYRMGDFYELFYEDAE
                     KAARLLDITLTQRGNSAGAPVKMAGVPYHSAEQYLAKLVKLGESVAICEQVGDPATSK
                     GPVERRVMRIVTPGTLTDAALLDEKAANLLMAACEDRGSVGLAWLNLASGELRIQQTN
                     AHAWIAELQRIGPAELLLPDGAEEPAMDRVCAVTRLPVWHFEHDAAHTLLTQHFGTKD
                     LAGFGADDMPVAVAAAGALLAYARKTQGQNLDHVVALLPERSDAYVRLDPATRRNLEL
                     TETLRGQPAPTLCSLLDRCATGMGSRLLRHWLHHPLRDRAVLNARLDAVAELGGDGVP
                     DLHSGLHAALRRVADVERIAGRIALKSARPRDLSSLRDTLAALPEIAAALNNAASEKL
                     QSLRDVLQSPPQILEQLQRAVAEEPSVVLREGGVIAPGYSAELDELRALQNNAGEFLI
                     ALEARERERTGIPNLKVEYNRVHGYYIEVTHAHSDKIPDDYRRRQTLKNAERYITPEL
                     KSFEDKALSAQERALALEKQLYETLLEDIAPAVPQLQAVARAIAELDVLCAFAALAAE
                     KSWHRPALVDENCVDIEAGRHPVVEEQVDDFIANDTRLSPTRRLLLITGPNMGGKSTY
                     MRQVALIALLAHIGSFVPAKSARLGPIDQIFTRIGAADDLAGGRSTFMVEMTESANIL
                     HNATERSLVLMDEVGRGTSTFDGLALAWSIARHLLEKNRALTLFATHYFELTQLAQEF
                     REAANVHLDAVEHKDRIVFLHALEEGPASQSYGLQVAALAGVPGTVIRAARRHLHLLE
                     QQSLSQDGQPDLFASTPEPVEPEPVTHPVVETLQAINPDDLSPREALDLIYKLKKL"
BASE COUNT         1426 a         2554 c         2409 g         1396 t
ORIGIN      
        1 cgcattggag tcaggaaagt catctctttc gtcatgcccg cgcaagcggg catgacgatc
       61 ggagttcccc tagatattga agctctcgcc gcagccgcac gaatccttca cgttcggatt
      121 attgaacttg aagccttcgt tcaaaccctc gcgcgcgaag tcgagttccg tgccgtcgat
      181 atacggcagg ctcttgggat cgacgatcac cttgatgccg ttgctctcga aggtgtggtc
      241 gtccggctgc acttcgtcgg cgaattcgag cttgtaggcc atgcccgagc agcccgaagt
      301 ctgcacgccc acacgcaggc cgatgccctt gccgcgcttg gccagaaagt cctgcacgtg
      361 cgtcgccgct ctatccgtca acgtaatcgc cataactact ccttaattac ttccagtctc
      421 agcctgcgtg aggctggtca ctgctcatgg tgttagcgtt caggcggaaa gtaaagtctg
      481 cgtcttttcc gtatgcgaat gcatgaccag cggcgccggc gccgtgccgt tctgctgcgc
      541 caccagatcg gccagcttca ccgattccag gtagtcgtac atgcgcacgt tcaactgcgc
      601 ccacaggtcg tgggtcatgc agcgcgtgtc cttgtggcag ttttccttgc cgccgcattg
      661 cgtcgcatcc agcggctcgt ccacggcaat gatgatgtcg gccaccgaca cgtgctcggc
      721 cggtcgcgcg agatagtagc ctccgcccgg gccgcgcacg ctctccacga tcttgtggcg
      781 gcgcagcttg ccgaataact gttccagata ggacagcgaa atgctctgtc gctggctgat
      841 ggccgccagc gggaccggtc cgctccccga attcagcgca aggtccaaca tggcggttac
      901 tgcgaaacgg cctttggtcg tcagtctcat tgcggaaact cctccagaag agccgtccag
      961 gacaccctct ccctggtccc agatggcggt cggagtatag ccatacccga ctatttttat
     1021 caagtattcc ggattttact tgtcggcgac cttgcgcagc accgggtcga agccggcgcc
     1081 gatggcctgg gcgtcctcct gggcacgcaa ttgctccagc tcgcgcgtca gggcttccag
     1141 gcggcgctcc agttcggcgg tgtggtcggt caggccgcga atcgcctgcg ccacggggtc
     1201 gtccacttcc ttggacaagc cgtatgcgga gaagcccagc cgttcggcgt gcgcttcccg
     1261 cgcctgctcg tgctggtcct cgatgatgcg cgccggaatg cccacggccg tggcgccggc
     1321 cggcacgtct ttgaccacga cggcgttgga gccgattttc gcgccgtcgc cgaccaggat
     1381 cgggccgagg atcttcgcgc ccgcgcccac gaccacgttc ttgcccagag tgggatggcg
     1441 cttgcccttg ttccaggacg tgccgcccag ggtgacgccg tgatagagcg tggtgtcgtc
     1501 gccgatttca gccgtctcgc cgatcaccac gcccatgccg tggtcgatga acacgcggcg
     1561 gccgatgacg gcgccggggt ggatctcgat gcccgtgaac cagcgcgcca catgcgaaag
     1621 cagacgcgca agccagcgga acttggcttt ccacagcttg tgcgccaggc ggtgcgacag
     1681 cagggcatga aaaccgggat aacaggtcag cacctcccag gtattgcgcg ctgcggggtc
     1741 gcgatcgaat acgctggcga tatcttctcg aatcctggcg aacatgtgag gcgccgatta
     1801 gggggttgaa ggggcggtat tatccctaaa acctagtttt ttgatcaact tttgcggcgg
     1861 tggttctgtt cccaggaagt cagaataccg cgcaagatgt tgacctcctc cttttccagc
     1921 cgcacgcgac cgtaaagacg gcgcaggcgc tccatcagcc gcttgggatg cgccgggtcg
     1981 aggaagccgc tttcggtcag gctgcgctca agatgggcat agaagccttc gatgtcctcg
     2041 taggcggccg catcgaagcg ttcgttgtcg atggacgggg cgccgagcgc ggccatgcgc
     2101 acctcatagg cgccgacctg taccgccgcc gccagattga gcgaggaata ctcgggattg
     2161 gccggaatgt gggcgatgcg ctggcacttg agcacgtcct cgttcgacag cccggacatt
     2221 tccgtgccga agacaagcgc cacctcgccc tgcccggcct cctcgaccag catgcgcgcc
     2281 gattcgcgca tgtccgccgc gatatgcgac agatcgcgcc gccgcgcagt cagcgcgacg
     2341 gcgaaacgcg tgcccgccag cgcctcgtcg agcgaggcgc atacccgggc gttggccagc
     2401 acgtcggccg cgccgctggc catcgcctcg gcttgcgggt cgggaaagcg tttgggattg
     2461 atcagcacca gacgcgacaa ccccatggtt ttcattgcgc gcgcggcggc accgatgttg
     2521 cccgggtgcg tggtgtggga gaggacgatg cgcacgcgtt cgagcgccgc gctcatggct
     2581 gtttccgatt cgaataaggc acgatgccgc tcacaggggc ccggacttga cgggcaagtt
     2641 agaatctcgt ctttcgctct tcgtataaag actcatgcac gcgcttcatc ccatgctcaa
     2701 cacagccatc aaggcggcac gacgcgccgg cgccgtgatc aaccgcgcct cgcgcgacct
     2761 cgatgtgctc tcggtgacgg ccaagcggcg caacgatttc gtgacggagg tcgaccgggc
     2821 ggcggaagaa gcggtgatcg aggtgttgcg tacggcttac cccgaccacg cgatcttagc
     2881 agaggagtcc ggcgcctccg gcacctccga gtacgagtgg atcatcgacc cgctcgacgg
     2941 caccaccaac ttcatccacg gcttcccgca gtacgcggtg tccatcgcgc tacgccagaa
     3001 gggcgtgatg cagcaggcgg tgatctacga tcccacgcgc aacgagttgt tcaccgccac
     3061 gcgcggacgc ggtgcctatc tcaacgatca gcgcatccgc gtctccaagc gcgcacaact
     3121 gaaggacagc ctgatcggta cgggcttccc gttccgcgag gtggacgaca tcgataccta
     3181 cctgcgcatt ttcaagcgcg tcaccttcga gacggcgggc atccgccgcg ccggcgccgc
     3241 cgcgctggat ctcgcctacg tggcgtgcgg gcgactggat gcgttctggg aaaaaggcct
     3301 tgcgccctgg gacatggcgg ccggcagtct gatcgtgctt gaggccggcg gcctgatcag
     3361 cgactacgac ggcgaagccg atttcatgga aagcggcgaa gtcgtgtgcg gcaaccccaa
     3421 ggtgttcgca caactgctgc aactggtgca gaagcagcgg cgcgcgcgga cgcctgccgc
     3481 cgggacgccc gccgccgaag agtaagtgcc acgaagtgtc atgcccgcgt aaacgggcac
     3541 tcgaaagccg ttcgcctcga gcgacatcat tcgcgcaggc gcaccaagcc gttcgccctg
     3601 acgtcgaagg gtgaacggcc gatactatgc tcgtcattcc ggccaagccc gcaggacgcg
     3661 agccggaatc cagtcaggcc gttcccgact ccggcgctcg tctgtcggct cccgactctc
     3721 gactgcagta ttagtcctgc accgccagca cttgcgcggc agcggccacg ctcgcggcga
     3781 tgcgaccgac gtcgcgcaac tgctgcaccg tcatgccggc ttccttcagc agcttgtagt
     3841 gcgagccgat gcagaagtgg cacttgccga tgatgctggc ggccagcgcg aacagctcga
     3901 agcggttgcg gtccacgccg ccgtgcgtcg cataggcgtt catgcgcagc tcggcgcgct
     3961 gcttcgacaa ctcctcgtcg cccgacattt ccacataggg ataccagacg ttgttcatgc
     4021 ccatcagcgc ggcagcggtg agcgcggcgt tgacgtccac gtccgacagt tcgccggaat
     4081 tgcgaatcgt atcgaccatg aacttgttgc cggtggcgta ggctgcggcc agcgcggcgc
     4141 cgagggcatc ggtcttctcc aggctggagc gcgcgatggt gccgtccaga ttgaggcgca
     4201 catccttggc gtagtccgga atgcgcgcct tgatggtgtt cagaaattcc atatcgacct
     4261 ctctcgaata ctgcacataa aaaatccgcg gacgggccgc ggattttcga ggtacggcgc
     4321 accgcggtgc gccgcacgcg gccggactta gagcgtctcg gcgcccactg cgcggttgca
     4381 ggggcacagt tcgtcggtct gcaggccgtc gaggatgcgc aagacttcct cggggctgcg
     4441 acccacgttc aggttgttga ccgacacgtg ctggatgacg ttgtgcggat cgacgatgaa
     4501 ggtcgcacgc agcgcgacgc cctcgttttt ctcgcggatg ccgagctgat cgaccagcga
     4561 acccgtcgta tcggcgaact gatagtggtt caggcccttc aggtcgggat gctcgcgacg
     4621 ccagccgagc ttgctgaatt cgttgtcggt ggaaccggcc atcagcaccg catcgcgctc
     4681 ctggaactga gcgttcagct tgccgaagcc gacgatttca gtgggacaca cgaaggtgaa
     4741 gtccttcggg tagaagtaga tgatcttcca cttgccgggg aacgacttct cgttgatctc
     4801 ttcgaaggcc gactggccct tctcttcatg ctggttgaaa ccgggcttga cgcctacgac
     4861 actgaatgct tcgagtttat cgcctacagt tttcatcaga gattgcttcc ttgcgttgaa
     4921 gcggctgaag ccgcgcacct aaaaacagac ggcgttaatg ctaggctatc gacaaccagc
     4981 cagcaatcgc actttccaat acgcgcgata ggtgccgtct aaaaatgaat ttgtttcaag
     5041 ggacgtgccc gttgcgcgat tggattgcgc agggcagtga cgccgattag accgttacgc
     5101 gcagcgatgg ttccgacgtg tgtgggtcgc cgccgttctc ggcgataatg cggacctcgt
     5161 tgcgcgccgg cgcacgaaaa aacgaacaca acgcgatcat gtcgtccagt acacccgccg
     5221 cacacacgcc gatgatgcag caatacctgg gattgaaagc ccagtatccg gatctgctgc
     5281 tgttctatcg catgggcgat ttctacgaac tgttttacga agatgcggaa aaagctgcac
     5341 gcctgctcga catcacactg acgcagcgcg gcaactctgc cggcgcaccg gtcaagatgg
     5401 ccggcgtgcc ctaccactcg gcggagcaat atctcgcgaa gctggtgaag ctgggcgaat
     5461 ccgtcgccat ttgcgaacag gtgggcgatc cggcgacgtc gaagggcccg gtcgaacgcc
     5521 gcgtgatgcg catcgtcacg cccggcacac tgaccgatgc ggcgctgctg gacgaaaaag
     5581 ccgcgaacct gctcatggcg gcatgcgagg atcgcggcag cgtcgggctc gcatggctca
     5641 atcttgccag cggcgaattg cgcatccagc agaccaacgc gcacgcgtgg atcgcggagt
     5701 tgcagcgcat cggccccgcc gaactgctgt tgcccgacgg cgccgaagaa ccggcgatgg
     5761 atcgtgtgtg cgcggtgaca cgtctacccg tctggcattt cgagcacgac gccgcgcaca
     5821 cgctgctcac gcagcatttc gggaccaagg accttgccgg cttcggcgcc gacgacatgc
     5881 ccgtcgccgt tgcggccgcc ggcgcactcc tcgcctacgc gcgtaagacg caaggccaga
     5941 atctcgatca cgtcgtcgcg ctgctgccgg aacgcagcga tgcctatgtg cgactcgatc
     6001 cggccacacg ccgcaacctc gaactcactg aaacgctgcg cggccagccc gcgccgacgc
     6061 tgtgcagtct gctcgaccgc tgcgccaccg gcatgggcag tcgcctgttg cgtcactggt
     6121 tgcaccatcc gctgcgcgac cgcgccgttc tgaatgcacg gctcgatgcc gtggcggaac
     6181 tcggcggcga cggcgtgccc gacctgcact cgggcctgca cgccgcgctg cggcgcgtgg
     6241 cggatgtgga gcgcatcgcc gggcgcatcg cgttgaagtc ggcgcgaccg cgcgatctgt
     6301 cgagcctgcg cgacacgctc gcggcgctgc ccgagatcgc ggccgcactg aacaacgccg
     6361 cgagcgaaaa gctgcaaagc ctgcgcgacg tgctgcagtc gccgccgcag atcctcgaac
     6421 aactgcaacg cgccgtcgcc gaagagccga gcgtcgttct gcgcgaaggt ggcgtcatcg
     6481 cccccggcta cagcgcggaa ctggacgagt tgcgcgcgct gcagaacaac gccggcgaat
     6541 tcctgatcgc actggaagcg cgcgagcgcg aacgcaccgg cattccgaac ctcaaggtcg
     6601 aatacaaccg tgtgcacggc tattacatcg aagtcaccca tgcgcacagc gacaagattc
     6661 cggatgacta ccgccgccgc caaacgctga agaacgccga gcgctacatc acgccggaac
     6721 tgaagagctt cgaggacaag gcgctctccg cgcaggaacg cgcgctcgcc ctcgagaagc
     6781 agctctacga aacactgctg gaggacattg cgcccgccgt gccgcaactg caggcggtgg
     6841 cgcgcgccat cgcggaactc gacgtgctgt gcgccttcgc cgccctcgct gcggagaaat
     6901 cctggcaccg ccccgccctg gtcgacgaaa actgcgtcga tatcgaagcc ggccgccatc
     6961 cggtggtgga ggaacaggtc gatgacttca tcgccaacga cacgcggctt tcgcccacgc
     7021 ggcgcctgct gctcatcacc ggcccgaaca tgggcggtaa atcgacttat atgcgccagg
     7081 tcgcgctcat cgccctgctc gcacacatcg gcagcttcgt cccggcgaag tcggcgcgcc
     7141 taggccccat agaccagatc ttcacgcgca tcggcgcggc ggacgatctc gccggaggcc
     7201 gctccacctt catggtggag atgaccgaaa gcgccaacat cctgcacaac gccaccgagc
     7261 gcagcctggt gctgatggac gaagtcggcc gcggcacgtc caccttcgac ggccttgcgc
     7321 tcgcctggtc catcgcgcgc catctgctgg agaaaaaccg cgcgctcacg ctattcgcca
     7381 cccactactt cgaactcacg caactcgcgc aggagttccg cgaagcggcc aacgtgcatc
     7441 tggacgcggt ggaacacaag gaccgcatcg tgttcctgca cgcgctggag gaaggccccg
     7501 ccagccagag ttacggcctg caggtcgcgg ctctggccgg cgtgcccggc actgtgatcc
     7561 gcgccgcgcg ccgacacttg catttgctcg agcaacaaag cctctcgcag gacggccagc
     7621 ccgatctgtt cgcttcgacg cccgaaccgg tcgaaccgga accggtcacg catcccgtcg
     7681 tcgagacgtt gcaggcgatc aatccggacg acctctcccc gcgcgaagca ctcgatctga
     7741 tttacaaact gaagaagctg taaagaagca gtggagagtc gagag
//
LOCUS       DATGOU010000143         8843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_1758490, whole genome shotgun sequence.
ACCESSION   DATGOU010000143 DATGOU010000000
VERSION     DATGOU010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8843
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1758490"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VKZ48_08245"
     CDS             <1..315
                     /locus_tag="VKZ48_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004535600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HLU77875.1"
                     /translation="YKVKGNIHWLSAAHAYESEVRLYDRLFRVPHPGAGDRDFLDDIN
                     PDSRKVITAYLEPGLKEAQPEERFQFERHGYFVADRVDSEPGAPVFNRAVTLRDSWAK
                     QK"
     gene            297..1112
                     /locus_tag="VKZ48_08250"
     CDS             297..1112
                     /locus_tag="VKZ48_08250"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006761631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HLU77876.1"
                     /translation="MGEAEIRQPWRAPGFIKASAALHVAGAAALVASPATWPWVLGAV
                     AANHAGITAAGLWPRSTLLGPNITRLPATAASRNEIALTFDDGPDPEVTPRVLDLLDA
                     RDAKASFFCIGKRATQNPQLVREIVARGHEVENHSQDHPVAFSFYGVGRFKRELAIAQ
                     QALTELTGRTPRYFRAPAGLRNPMLDPALHALGLQLVSWTRRGFDGVSGDAETVYTRL
                     TRGLASGDILVLHDGHPPRTSDGTPVVLAVLPRLLDALAQRGLRAVRLTQGLA"
     gene            1109..1819
                     /locus_tag="VKZ48_08255"
     CDS             1109..1819
                     /locus_tag="VKZ48_08255"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLU77877.1"
                     /translation="MSDGAVAALLRGAARRYLVCGRTVMHFMHGKLRHDPIFVELLRT
                     GALRSASSLLDLGCGRAPLFALLLEAQVRRANDTWPAGWPVPPSFEQMRGIELSRHDV
                     QIARRALAPHAEIVDGDLTEIALDRAEVITLFDVLHYLVPREQERLIDRIVHALDGAG
                     TLLMRVGNPAAGWRHAFTQAVDRTVWALRGHPFDALHCRDVDAWIALLRRHGLRVDAA
                     PMHQGTPFANVLLIARRH"
     gene            1877..3736
                     /gene="treZ"
                     /locus_tag="VKZ48_08260"
     CDS             1877..3736
                     /gene="treZ"
                     /locus_tag="VKZ48_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HLU77878.1"
                     /translation="MQHLHTGHDCGARPAGDAVHFEVWAPDAKSVDVELNGAHHIMRR
                     DVDGYYTATVQGAKAGDLYRYRIDGDRSYPDPCSRYQPEGVHGPSMVVDPGAFRWTDQ
                     AWPGVELEGQVIYEMHIGTFTPEGSFDAAIREFTELRALGITMLEVMPIAEFPGRFNW
                     GYDGVDLYAPYHGYGDAEAFKRFVDAGHAAQLAVILDVVYNHLGPDGNYLGCYGKQYF
                     TDRYANEWGEALNFDGEGAGPVREFFIRNACYWIGEFHLDGLRLDATQSIHDASPRHV
                     LAELSERARAAAGARKIVLIAENEPQNTRQLKPVAAGGYGLDGMWNDDFHHSARVAMT
                     GRHEGYFHDHRGRPQEFISAVKYGFLFQGQYYHWQKQPRGTPTTGLPACAFVAFVQNH
                     DQVGNTLYGARLGAMTSPGRARALTALLLLAPQTPMLFMGQEYGASQPFTFFADHNPE
                     LARLVHRGRREFVAQFKPYATERAQERVPDPADVATFNRCKLDFSERESHRPLYDLHR
                     DLLHLRRTDPVIARQDRTRIDGAVLASDAFVLRWFDETHGDRLLVVNFGDEAEIHPAP
                     EPLLAPPEGQEWLLQWSSDEPHYGGFGVVNPCGEAGWVIPGESAALLQPTPKS"
     gene            3740..5788
                     /locus_tag="VKZ48_08265"
     CDS             3740..5788
                     /locus_tag="VKZ48_08265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HLU77879.1"
                     /translation="MRSTSASIQIDWRAGDDPVQLRTREWLVTNGLGGFASGTLLGPA
                     TRRYHGLFVPNLADPEGRYVLISRLDEEVIAGDTRVHLGGAEYLDERLACDTQHHLRA
                     FRFAHLMPTWRYAVDDRVLEKTIVMSHMQNTVFVQYKLLRGEPLRLQVRPFVAFRRQD
                     AVLEPRDYSAFKLSIDGNEHEVQLQDAPLSLRFGMRPTDCAFVADAVEEHDVLFRKER
                     DRGYDHSENQYSPGYFALDLQADAPATFVATVHGWEALNIDAGQAIEAERNRVERLLA
                     IAPASARSGFPAQLVMAADQFLVIPGSRLEDALLARASGGELRTVIAGYHWFGDWGRD
                     TMISFEGLTLCTGRHREAGAILRTFAHYVRDGLLPNLFPEGAREALYHTVDATFWFFH
                     AIERYRHVVGDEAIVAELWPTLCSIIDHHVRGTRYGIGVDPADGLIRAGAPGYQLTWM
                     DAKVDDWVVTPRRGKPVEIQALWHNALSLMAEWASELHNDPAPFRERAALARRSFNAR
                     YWNEEAGHLYDVIDGEDGSDDPACRPNQIFSISLSHPVLDPAHWEAVVETVHQQLLTP
                     YGLRTLNPEHPDYKRTYEGDLRSRDAAYHQGTVWPWLIGHYLDAWIKVHGRDERAGEL
                     LQAFPEHLRDAGIGSISEIFDATHPYVPRGCIAQAWSVAEVLRAWLKTTGDVGAPPSA
                     "
     gene            6091..7506
                     /locus_tag="VKZ48_08270"
     CDS             6091..7506
                     /locus_tag="VKZ48_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018421747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLU77880.1"
                     /translation="MTSSFNTLGLSAEILRAVNEQGYTQPTPVQAQAIPVIIEGRDVM
                     AGAQTGTGKTAGFTLPMLQRLSINATSSTSPAKRPVRALILTPTRELAAQVHDSVRAY
                     GKYLRLKSTMIYGGVGMRPQIDALRRGVDIVVATPGRLLDHVQQRTIDLSKVELLVLD
                     EADRMLDMGFIHDIKRVMALLRKDRQNVLFSATFSGEIRKLADSLLNDPVVIEVARRN
                     QESELITQRIHPVDHARKRELLTHLISEGEWQQVLVFARTKHGADRLADQLSRANIEA
                     AAIHGNKSQGQRTRALAQFKRGAVRVLVATDIAARGIDIEELPHVVNYELPNVAEDYV
                     HRIGRTGRAGSSGMAVSLVSREEAPLMRDIERLLKRKLDREIVVGFEPTDAFDPAALP
                     NRRPQGRKPQGGRPNGGKPGFGKSSSGKPGFGGKPSFGGDKPAFGRHNAGGGKPRTEG
                     QRSEHRRTGGRPQTAALLRGN"
     gene            7830..>8843
                     /locus_tag="VKZ48_08275"
     CDS             7830..>8843
                     /locus_tag="VKZ48_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003814283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HLU77881.1"
                     /translation="MSKQSSERIVIRGARQNNLKNLSLDIPTNQLVVVTGVSGSGKSS
                     LVFDTLYAEGQRRYVETFSPYARQFLDRQDRPQVDAIEGIPPAIAIDQTNPVRTSRST
                     VGTMTELNDHLKLLYARGAHLHCRRCARPVRHDTSDTIYADLQQRTAEAGDPRLLVVF
                     PVEIPANFSEAEVKQLLEQQGYTRFHAESALRIEVVQDRFRFGNAERARVIEAIEAAL
                     KVGKGHVYVYVVNDEGATRDCWRYSAGLHCAHCNIHYSQPTPSHFSFNSPLGACEECR
                     GFGRVIGIDYGLVIPDESKTLREGAIRPWQTESFRECQDDLVKYAKKRGIPLDVPYRD
                     LSDE"
BASE COUNT         1560 a         3079 c         2745 g         1459 t
ORIGIN      
        1 tacaaggtga agggtaacat ccactggctg tccgccgccc acgcctatga aagcgaagtg
       61 cggctgtacg accgcctgtt ccgcgtcccg catcccggcg cgggcgatcg cgacttcctc
      121 gacgacatca atccggattc caggaaagtg atcaccgcct atctggaacc gggattgaaa
      181 gaggcgcagc ccgaagagcg cttccagttc gagcgacacg ggtattttgt agctgaccgc
      241 gtggattccg agccgggcgc acccgtcttc aaccgtgcag taaccctgcg cgactcgtgg
      301 gcgaagcaga aataagacag ccctggcgcg cgccgggctt catcaaagcc tcggcggcgc
      361 tgcatgtcgc cggcgcggcg gcactcgttg cctcgcccgc cacctggccg tgggtgctgg
      421 gcgcggtcgc ggccaatcac gccggcatta ccgccgcggg tctttggccg cgcagcacat
      481 tgctcggccc gaacatcacg cggctgcctg cgacggccgc ctcgcgcaat gaaatcgcgc
      541 tgaccttcga cgacggcccc gatcccgagg tcacgccgcg tgtgctcgac cttctcgatg
      601 cgcgcgacgc gaaagcgagc ttcttctgca tcggcaaacg cgcgacacaa aatccgcagc
      661 tcgtgcgcga aatcgtcgcg cgcggccacg aggtcgagaa tcacagtcag gaccatcccg
      721 tcgcattcag cttctacggc gtcgggcgtt tcaaacgcga acttgccatt gcgcaacaag
      781 cgctcaccga actgaccggc cgcacgccgc gctacttccg cgcgccggcc ggcttgcgca
      841 atcccatgct cgatcccgcg ctgcatgcgc tcggcctgca gctcgtttcc tggacgcggc
      901 gcggcttcga cggcgtatcc ggcgatgcgg aaaccgtgta cacacgcctg acgcgcggac
      961 ttgcttcggg cgacatcctc gtcctgcatg acggccatcc gccgcgcacg tccgacggca
     1021 cgcccgtcgt cctggcggta ctgccgcgcc tgctcgatgc actggcgcaa cgcgggctgc
     1081 gggccgtccg cctcacgcaa gggctcgcat gagcgacggt gccgtcgctg cattattgcg
     1141 cggcgcggca cggcgatacc tcgtttgcgg ccgcacggtc atgcacttca tgcacggcaa
     1201 gctgcgccac gaccccatct tcgtcgaact gctgcgcacc ggtgcgttgc gcagcgcatc
     1261 gtcgctgctc gatctgggct gcggccgcgc gccgctgttc gcactgctgc tcgaagcgca
     1321 ggtacgccgc gcaaacgaca cgtggcccgc cggctggccc gtaccgccgt ctttcgaaca
     1381 gatgcgcggc atcgagctga gtcggcacga tgtacagatc gcccgacgcg cactcgcacc
     1441 gcatgccgaa atcgtggacg gcgatctcac ggagatcgca ctggatcgcg ccgaagtgat
     1501 cacgctgttc gacgtgctgc attacctcgt gccccgcgag caggaacgcc tgatcgaccg
     1561 catcgtgcat gcgctcgacg gcgcgggaac gctgctcatg cgcgtgggca atccggccgc
     1621 cggctggcgc cacgctttca cgcaggcggt cgatcgcacg gtctgggcgc tgcgcggcca
     1681 tcccttcgat gcgctgcact gccgtgacgt ggacgcctgg atcgctctgc tgcgtcgcca
     1741 cggcttgcgc gtcgatgccg cgcccatgca tcagggcaca cccttcgcca acgtgctgtt
     1801 gatcgcgcgg cgacactgac ggcgcacggg agcgaatctt gccgtcccgg cttggggcgg
     1861 caaggatttc tccaccatgc aacatctaca caccggccac gactgcgggg caagacctgc
     1921 gggtgacgcc gtgcacttcg aagtctgggc gcccgacgca aagtcggtcg atgtggaatt
     1981 gaacggcgcc caccacatca tgcggcgcga cgtcgacggg tattacacgg cgacggtgca
     2041 gggcgcgaag gccggcgact tgtaccgcta ccgcatcgac ggcgaccgca gttatcccga
     2101 tccctgttcg cgctatcagc ccgaaggcgt gcacggcccg tccatggtcg tcgatccggg
     2161 cgccttccgc tggaccgatc aggcttggcc cggcgtcgaa ctcgagggcc aggtgatcta
     2221 cgaaatgcac atcggcacct tcacgcccga aggcagcttc gatgcagcca tccgcgagtt
     2281 caccgaactg cgcgccctcg gcatcaccat gctcgaagtg atgccgatcg cggaatttcc
     2341 cggacgcttc aactggggct acgacggcgt cgatctttat gcgccctatc acggctacgg
     2401 cgatgcggag gccttcaagc gtttcgtcga tgcggggcac gcggcccagc tcgccgtcat
     2461 tctcgacgtg gtctacaacc atctcggacc ggacggaaat tatctgggct gctacggcaa
     2521 acagtacttc accgaccgct acgccaacga atggggcgaa gcgctcaact tcgacggcga
     2581 aggcgccggg ccggtacgcg aattcttcat ccgcaatgcc tgctactgga tcggcgagtt
     2641 ccacctcgac ggcctgcgcc tcgatgcgac gcaaagcatc cacgacgcga gcccccgaca
     2701 cgtgctcgcc gagctgtccg aacgcgcacg cgccgcggcg ggtgcacgca agatcgtgct
     2761 catcgccgag aacgaaccgc agaacacgcg tcagttgaaa cccgtcgccg cgggcggcta
     2821 tggactggac ggcatgtgga acgacgactt ccatcattcc gcgcgggtgg cgatgaccgg
     2881 ccgccacgaa ggctacttcc acgatcaccg cggccgcccg caggaattca tctcggcggt
     2941 gaagtacggc ttcctgttcc agggacagta ctaccactgg cagaagcagc cgcgcggcac
     3001 gcccaccacc ggtctgcccg catgcgcctt cgtcgcgttc gtccagaacc acgatcaggt
     3061 cggcaacacg ctgtacggtg cgcgtctcgg cgcgatgacc agccccggtc gcgcacgcgc
     3121 gctcaccgcc cttctcctcc tcgcgccgca gacgccgatg ctgttcatgg gccaggaata
     3181 cggcgcgagc caacccttca ctttcttcgc cgaccacaac ccggaactcg cgcgactggt
     3241 ccaccgcgga cgccgcgaat tcgtcgcaca gttcaagccc tatgccaccg agcgtgcgca
     3301 ggaacgggtg cccgacccgg cggacgtggc gacgttcaac cgctgcaagc tcgatttctc
     3361 cgagcgcgaa agccatcgcc cgctctacga cctgcaccgc gatctgctgc acctgcggcg
     3421 cacggacccg gtcatcgcac gacaggaccg cacgcgcatc gacggcgcgg tccttgcgag
     3481 cgatgccttc gtgctgcgct ggttcgacga gacgcacggc gaccggctgc tggtggtgaa
     3541 cttcggcgac gaggcggaaa tccatcccgc gccggaaccg ctgctcgcgc cgcccgaagg
     3601 gcaggaatgg ttgctgcaat ggtccagtga cgagccgcat tacggcggct tcggcgtggt
     3661 caatccttgc ggcgaagccg ggtgggtgat tcccggcgaa agcgccgccc tgttgcaacc
     3721 gactcccaaa tcctgattga tgcgatcgac ttcggcttcc atccaaatcg actggcgcgc
     3781 gggcgatgat cccgtgcaat tgcgcactcg cgaatggctg gtgaccaacg ggctcggcgg
     3841 tttcgcctcc ggcacgctgc tcggcccggc gacgcggcgg tatcacggtc tgttcgtgcc
     3901 caacctggcc gatccggagg gccgctacgt cctgatctcc cggctggatg aagaagtgat
     3961 cgccggcgat acgcgcgtcc acctgggcgg cgccgagtat ctggacgaac ggctggcctg
     4021 cgacacgcag caccatctgc gcgcattccg cttcgctcac ctcatgccga cctggcgcta
     4081 cgccgtcgac gatcgcgtgt tggaaaaaac cattgtgatg tcgcacatgc agaacaccgt
     4141 gttcgtgcag tacaagctgc tgcggggcga gccgctgcgg ctacaggtgc ggcctttcgt
     4201 ggcattccgt cggcaggacg ccgtactgga gccgcgcgac tactcggcat tcaagctgtc
     4261 catcgacggc aacgaacatg aagtgcaact gcaggacgcg ccgttgagcc tgcgcttcgg
     4321 catgcgcccg accgattgcg cattcgttgc ggacgccgtg gaggagcacg acgtgctctt
     4381 tcgcaaggaa cgcgaccgag gctacgacca cagcgagaac caatacagcc ccggttactt
     4441 cgcgctagat ctgcaagccg acgcgcccgc caccttcgtc gccaccgtgc acggctggga
     4501 agcgctgaac atcgacgcgg ggcaggccat cgaagcggaa cgcaaccgcg tcgaacgcct
     4561 gctggccatt gcgccggcga gtgcgcgcag cggctttccg gcgcaactgg tcatggccgc
     4621 cgaccagttc ctggtcattc ccggcagccg gctggaagac gcgttgctcg cgcgcgcctc
     4681 gggcggcgag ttgcgcaccg tcatcgccgg ctatcactgg ttcggcgact ggggccgcga
     4741 caccatgatc agcttcgagg ggctgacgct ctgcaccggc cgccatcgcg aagcgggcgc
     4801 gatcctgcgc accttcgcgc attacgtgcg cgacggcctg ctgcccaatc tgttcccgga
     4861 aggcgcacgc gaagcgcttt atcacaccgt cgatgccacc ttctggttct tccatgccat
     4921 cgagcgctat cgtcatgtgg tcggcgacga ggcgatcgtc gcggagctgt ggcccacgct
     4981 gtgcagcatc atcgatcatc acgtgcgcgg cacacgctac ggcatcggtg tcgatccggc
     5041 ggacgggctc attcgcgccg gcgcacccgg ctaccagctc acctggatgg acgccaaggt
     5101 cgacgactgg gtggtcacgc cgcggcgcgg caaaccggtg gagatccagg cgctatggca
     5161 caacgcgctc agcctcatgg cggagtgggc gtcggagctg cacaatgatc cggcaccgtt
     5221 ccgcgagcgc gcggcgctgg cgcggcgcag tttcaatgcg cgctactgga acgaagaggc
     5281 cggtcatctc tacgacgtga tcgacggcga ggacggcagc gacgaccccg cctgccgccc
     5341 caaccagatt ttcagcattt cgctgtcgca tcccgtcctc gatccggcgc actgggaggc
     5401 ggtggtggag acggtgcatc agcaactgct cacgccctac ggtctgcgca cactgaatcc
     5461 cgagcatccc gactacaagc gcacctacga aggcgatctg cgctcgcgcg acgccgccta
     5521 ccaccagggc acggtgtggc cgtggctgat cggccactac ctcgacgcct ggatcaaggt
     5581 acacggccgg gacgaacgcg cgggcgaact gctgcaggct tttcccgaac acttgcgcga
     5641 cgcgggcatc ggcagcatca gcgaaatctt cgacgccacg catccctatg tgccgcgcgg
     5701 ctgcatcgcc caggcgtgga gcgtggcgga agtgctgcgc gcctggctga agacgaccgg
     5761 cgacgtcggc gcgccgccct cggcgtgatt cggctcgccg ctttatttgt ccggacaccg
     5821 aataagaccg tacgaagccg gcaaaagcct gtatagtgcg cgcacggatt caccgatcgc
     5881 ctcatccggt ctgcgatcga atcccctcgc cactgcactt ccgcacgcga ttcacttcca
     5941 accaaaagaa ttcagcctga accggttctg tcgaccttgt gtcggtagca ggtcgatagg
     6001 gaacggcaca ggcctatccg tgcgcgtttg acgcgccgtc tttcgctttt gctcgctccc
     6061 gccagggcgc atagcgccag gagcattgct ttgacttcca gtttcaacac cctcggtctg
     6121 tcggccgaga ttctgcgtgc cgtcaacgaa cagggctaca cgcagcccac ccccgtccag
     6181 gcgcaagcca tccccgtcat catcgaaggc cgtgacgtca tggccggcgc acagaccggc
     6241 acgggcaaga ccgccggctt cacgctgccg atgctgcagc gtctgtcgat caacgcgacc
     6301 agcagcacct cgccggccaa gcgtccggtg cgtgcgctga tccttacgcc gacgcgcgaa
     6361 ctcgccgccc aggtgcacga cagcgtgcgt gcctacggca agtatctgcg cctcaagtcc
     6421 accatgatct acggcggcgt cggcatgcgc ccgcagatcg acgcgctgcg tcgcggtgtg
     6481 gacatcgtcg tcgccacgcc cggccgcctg ctcgatcacg tgcagcaacg caccatcgat
     6541 ctctcgaagg tcgaactgct tgtgctggac gaagccgacc gcatgctcga catgggtttc
     6601 atccacgaca tcaagcgcgt tatggcactg ctgcgcaagg accgccagaa cgtgctgttc
     6661 tccgccacct tctccggcga gatccgcaag ctggccgact cgctgctgaa cgatccggtc
     6721 gtgatcgaag tggcgcgccg caatcaggaa tccgagctga tcacgcagcg catccacccg
     6781 gtggatcacg cgcgcaagcg cgagctgctg acgcatctca tctccgaagg cgaatggcag
     6841 caggtgctgg tgttcgcacg caccaagcac ggcgccgacc gcctggccga tcagttgagc
     6901 cgcgccaata tcgaagctgc cgccattcac ggcaacaagt cgcagggtca gcgcacccgc
     6961 gcactggcgc agttcaagcg cggcgcggtg cgcgtgctgg tggcgaccga catcgccgca
     7021 cgcggcatcg acatcgaaga actgccgcat gtcgtgaact acgaactgcc caacgtggcg
     7081 gaagattacg tccaccgcat cggccgcacc ggccgcgcag gcagcagcgg catggcagtc
     7141 tcgctcgtgt cgcgcgagga agcgccgctc atgcgcgaca tcgaacgcct gctgaagcgc
     7201 aagctggatc gcgagatcgt cgtcggcttc gagccgaccg atgcgttcga ccccgcggcg
     7261 ctgcccaatc gccgtccgca aggccgcaag cctcaaggcg gacgtcccaa cggcggcaaa
     7321 ccgggcttcg gcaagtcctc gtcgggcaaa ccgggattcg gcggcaaacc gtctttcggc
     7381 ggcgacaagc ctgccttcgg ccggcacaac gccggcggcg gcaagccgcg cacggaaggc
     7441 cagcgttccg aacaccgccg caccggcggc cgtccgcaaa cggcggcgct gctgcggggg
     7501 aactgaggtc cgccgtttgt cctgagcgtg cctgtcctga gcttgccgaa ggatcgaagg
     7561 atgagcggcc ctgcgccaac cttccgttca cccttcgact agctcagggc gaacgggagt
     7621 ttgagctggc cagcgcatca gcaagccgtt cgtggtgagg ctttcccagg ccgttcgtgg
     7681 tgagcctgtc gaaccatgaa cggccacgcg ccattcgtcc ttcgacaagc tcaggacgaa
     7741 cgggcggtga gacgactcgt ttaaaatacg ggtcacccgc ggcgcctacg ggcgccgttt
     7801 tcatttgtgc tgcgcctcgc gcctgatcca tgtccaagca gtcgtccgag cgcatcgtca
     7861 tccgcggtgc gcgtcaaaac aatctaaaga acctcagcct cgacatcccc accaaccagc
     7921 tcgttgtcgt gaccggcgta tcgggctcgg gtaagtcctc gctggtgttc gacacgctct
     7981 acgccgaagg ccagcgccgc tacgtcgaga ccttctcgcc ctacgcgcgc cagttcctgg
     8041 accggcagga tcggccgcag gtcgatgcca tcgaaggcat cccgcccgcc atcgccatcg
     8101 accagaccaa tccggtgcgc acctcgcggt ccacggtcgg cacgatgacg gaactgaacg
     8161 atcatctgaa gctgctctac gcccgtggcg cgcatctgca ttgccgccgc tgcgcgcgcc
     8221 cggtacggca cgacacctcc gacaccatct acgccgatct gcagcagcgc accgccgagg
     8281 cgggcgaccc gcgcctgctg gtcgtcttcc cggtcgaaat cccggcgaac ttcagcgaag
     8341 cggaagtgaa gcagttgctg gagcagcagg gctacacgcg cttccacgcc gaaagcgcac
     8401 tccgcatcga agtcgtgcag gaccgcttcc gtttcggcaa cgccgaacgg gcgcgcgtaa
     8461 tcgaggcgat cgaggcggcg ctcaaggtgg gcaagggtca cgtttacgtc tatgtggtga
     8521 acgacgaggg cgcgacgcgg gactgctggc gttacagcgc cggcctgcat tgcgcacact
     8581 gcaacatcca ctactcgcag ccgacgcctt cgcatttctc gttcaactct ccgctcggcg
     8641 cctgcgagga atgccgcggc ttcgggcggg tgatcggcat cgactacggc ctcgtcattc
     8701 ccgacgaaag caagacgctg cgcgaaggcg cgatccgtcc ctggcagacg gaaagcttca
     8761 gggaatgcca ggacgacctg gtgaagtacg ccaagaaacg cggcatcccg ctcgacgtgc
     8821 cctatcgtga tctctccgac gaa
//
LOCUS       DATGOU010000144         2615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_4409918, whole genome shotgun sequence.
ACCESSION   DATGOU010000144 DATGOU010000000
VERSION     DATGOU010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4409918"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VKZ48_08280"
     CDS             complement(<1..804)
                     /locus_tag="VKZ48_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011154158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HLU77882.1"
                     /translation="MLHEALLADLSTELPHAVRLQRLVSTMRAHFHCGAVVLLRLDGD
                     CLCPVAVDGLVRETLGRRFVVAQHPRLAAILASRGPTRFDPGSPLPDPYDGLLDSHVG
                     EPLPVHDCMGTSVYVEGNLWGALTLDALHAGTFDEQMREELQRYNLLIEAAVRVTRLE
                     QESRSLRLARDYHPAAGDTVPSDESEILGHSAAIQRLLHELDVVADSELPVLLLGETG
                     VGKELFARRLHRQSRRADAPLVHVNCAALPESLAESELFGHVKGAFSGAT"
     gene            949..1620
                     /gene="ytfE"
                     /locus_tag="VKZ48_08285"
     CDS             949..1620
                     /gene="ytfE"
                     /locus_tag="VKZ48_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HLU77883.1"
                     /translation="MSIYDLTIAQIARDIPGATRVFRHYDLDFCCRGGQSLRDAAKER
                     GLDEHTIGDALEALITRPSSERDWRNAEPRELIQHILARYHAVHREQLPEMIRLAQRV
                     EEVHGARADCPAGLTDLLREMQQELESHMMKEEQILFPMLSRGRGPVVCGPITVMMME
                     HEQHGASLDRMCALTNDITLPEGACNTWRALYGALAQFRDDLMDHIHLENNILFASAT
                     EPLHV"
     gene            1625..>2615
                     /locus_tag="VKZ48_08290"
     CDS             1625..>2615
                     /locus_tag="VKZ48_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019449105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HLU77884.1"
                     /translation="MKTYKKLWWLLIAILAVTFTLLGYLGREVYRQAPPIPDRIVTTD
                     GTLISTSTKILDGQTAYQSVGGMQLGSIYGHGAYQAPDWTADWLHRELLAWLDIAAQE
                     EYGTPYAQLDTEAQQTLQHRMKLEYRKNTYDEAAGTATITPRRAAAMAAVAKHYDALF
                     GGDAALQGTRESYAMKEVTLPDAKRRDDLSAFFAWTAWTAATERPGHDATYTNNWPHE
                     PLIDNRPTAENIIWSIASVVLLVAGVGLLIWAWAFLRKHEEDEPPAPARDPLTTFALT
                     PSQKALGKYLFLVVALFIFQVLLGGMTAHYTVEGQTFYGIQLSEWFPYSLTRTW"
BASE COUNT          502 a          879 c          796 g          438 t
ORIGIN      
        1 cgtggcgccg gagaaggcac ctttgacgtg gccgaacaat tcgctttcgg cgagcgactc
       61 gggcagcgcg gcgcagttga cgtgcacgag cggcgcgtcg gcgcgacgcg actggcgatg
      121 cagtcgccgt gcgaacaatt ccttgcccac gccggtttcg ccgagcaaca gcaccggcag
      181 ttcggaatcg gccaccacgt ccagttcgtg cagcagacgc tggatcgccg cgctgtgccc
      241 gaggatctcc gactcatcgc tcggcacggt atccccggcg gcaggatgat agtcgcgcgc
      301 gaggcgcagg ctgcggcttt cctgttccag gcgcgtcacg cgtaccgccg cttcgatgag
      361 caggttgtag cgctgcagtt cctcccgcat ctgttcgtcg aaggtgccgg catgcagcgc
      421 gtccagcgta agcgcgcccc acagattgcc ttccacatac acgctggtgc ccatgcagtc
      481 gtgcacgggc aaaggctcgc cgacgtgact gtcgagcaag ccgtcgtagg gatcgggcaa
      541 cggactgccc ggatcgaagc gcgtcggccc gcggctggca agaatggcgg ccagacgcgg
      601 atgctgcgcg acgacgaagc gccgccccag cgtttcgcgc accagtccat ccaccgccac
      661 cgggcagagg cagtccccgt ccagacgcag cagcacgacc gccccgcaat gaaagtgcgc
      721 gcgcatggtc gagaccagcc gctgcagccg gacggcatgc ggcaattcgg tggagaggtc
      781 ggccaataag gcttcgtgca gcatggttat ttttaccgcg tgatggtttt ttgaacccta
      841 ttacaaaatg gtgtttttca ccagaataat tttttcttct tcaatttcag tgcactaaaa
      901 ctgtcacgac acttgctaat ggctgttccg agtagaggag caaaagccat gagcatttat
      961 gacctgacca ttgcccagat cgcccgggat attcccggcg ccacacgggt gtttcgtcac
     1021 tacgacctgg atttctgctg ccgcggcgga caaagcctgc gcgacgcggc caaggaacgc
     1081 ggtctggacg agcacaccat cggcgacgcc ctggaagcgc tgatcacgcg ccccagcagc
     1141 gagcgcgact ggcgcaacgc cgaaccgcgc gaactgatcc agcacattct cgcccgctac
     1201 cacgcggtgc atcgcgaaca actacccgaa atgatccggc tcgcgcagcg cgtcgaggaa
     1261 gtgcacggcg cgcgcgccga ctgccctgcc ggtctgaccg acctgctgcg cgaaatgcaa
     1321 caggaactgg aaagccacat gatgaaggaa gagcagattc tcttccccat gctttcgcgc
     1381 ggtcgcggcc cggtggtgtg cggtcccatc accgtcatga tgatggagca cgaacagcac
     1441 ggcgcatcgc tcgaccgcat gtgcgctctc accaacgaca tcacgctgcc ggaaggcgcg
     1501 tgcaacacct ggcgcgcgct gtacggcgcg ctcgcccagt tccgcgacga cctgatggat
     1561 cacatccatc tcgagaacaa cattcttttc gcatccgcaa cggagccctt gcatgtctaa
     1621 cccaatcaaa acctataaga agctttggtg gctgctcatt gccattcttg ccgtcacctt
     1681 cacgctgctc gggtacttgg ggcgtgaagt ctatcggcag gcgccgccga ttcccgatcg
     1741 catcgtcacg accgacggca cactgatctc gacgtcgacg aagattctcg acggccagac
     1801 cgcctatcaa tccgtaggcg gcatgcaact gggttcgatc tacggtcacg gcgcctatca
     1861 ggcgcccgac tggacggcgg actggctgca ccgcgaactc ctggcgtggc tggacatcgc
     1921 cgcgcaggag gaatacggca cgccgtacgc gcagctcgac accgaggcgc agcagacgct
     1981 gcagcaccgg atgaagctcg agtaccgcaa gaacacctac gacgaggcgg ccggcaccgc
     2041 gaccatcacg ccgcggcggg cggcggccat ggcggcggtg gcgaagcact acgacgcgct
     2101 gttcggcggt gatgccgcgc tgcagggcac gcgcgaaagc tacgccatga aggaggtcac
     2161 gctgcccgac gcgaagcggc gcgacgacct gtcggccttc ttcgcctgga ccgcgtggac
     2221 ggctgcgacc gaacgccccg gccacgatgc gacctacacc aacaactggc cgcacgagcc
     2281 gctgatcgac aaccgtccta cggcggagaa catcatctgg tcgatcgcca gcgtggtgct
     2341 gctcgtcgcc ggcgtgggcc tgctgatctg ggcctgggcc ttcctgcgca agcacgaaga
     2401 ggacgagccg cccgcacccg cgcgcgaccc cctcaccacc ttcgcgctca cgccctcgca
     2461 aaaggcactg ggcaagtatc tgttcctggt ggttgcgctg ttcatcttcc aggtcctcct
     2521 gggcggcatg accgcgcact acaccgtgga aggacagacg ttctacggaa tccagctttc
     2581 ggagtggttc ccctactcgc tcacccgcac ctggc
//
LOCUS       DATGOU010000145        11400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_5759542, whole genome shotgun sequence.
ACCESSION   DATGOU010000145 DATGOU010000000
VERSION     DATGOU010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 11400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11400
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5759542"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VKZ48_08295"
     CDS             <1..239
                     /locus_tag="VKZ48_08295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLU77885.1"
                     /translation="GVRLSIQDNGIGMPATIAADALSHGILGMRERVMGLGGVFRIES
                     PPEGGTRIEVSVPMTHDPTTSSAPTDAPAELQSV"
     gene            complement(659..856)
                     /locus_tag="VKZ48_08300"
     CDS             complement(659..856)
                     /locus_tag="VKZ48_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HLU77886.1"
                     /translation="MNKDRIEGNWKQLRGKVKEQWGKLTDDQLDMIEGKREQLSGKIQ
                     EAYGISREEADKQVSDWERGI"
     gene            complement(989..1150)
                     /locus_tag="VKZ48_08305"
     CDS             complement(989..1150)
                     /locus_tag="VKZ48_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010120894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HLU77887.1"
                     /translation="MLNWAIAFFIIAIIAAIFGFGGIAAGAAEIAKILFFVFLVLFLV
                     SLITGLLRR"
     gene            complement(1265..1402)
                     /locus_tag="VKZ48_08310"
     CDS             complement(1265..1402)
                     /locus_tag="VKZ48_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HLU77888.1"
                     /translation="MTMLKAVSALLLVMFALIGCNTMEGAGQDIQRGGEKLENAADRN
                     K"
     gene            complement(1478..1930)
                     /locus_tag="VKZ48_08315"
     CDS             complement(1478..1930)
                     /locus_tag="VKZ48_08315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLU77889.1"
                     /translation="MNGKNGGSQVSAAPRSLPVRVLLIEDSAIIRERLAESIVEPGRI
                     EIVGQADCEGDALRALRDTPCDAVVLDLQLKQGSGLGVLKSARARPGEPVYIVFTNYD
                     FPHYREKSAQLGASFFFDKAREHARVREVLEDMARAREEACAAKHIAS"
     gene            complement(1927..2592)
                     /locus_tag="VKZ48_08320"
     CDS             complement(1927..2592)
                     /locus_tag="VKZ48_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014190938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLU77890.1"
                     /translation="MQATTRIPPSRIRILIADDHAIVRAGLKQFIADEPDMAVTGEAE
                     TGSQTLDLVRNGEWDVVLLDISMPDRSGVDTLKSLKHVKPELPVLILSGFPEGQYAVN
                     LLRAGASGYLNKESAPAELVRAIRTVVAGRRYVSSTLAEILAHDVSSPASERPLHTAL
                     SEREFQIFCKLAAGQAVSKIAEELFLSVKTVSTYRTRILEKMGMKTNADLTYYAIKNQ
                     LIQ"
     gene            complement(2630..3688)
                     /locus_tag="VKZ48_08325"
     CDS             complement(2630..3688)
                     /locus_tag="VKZ48_08325"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLU77891.1"
                     /translation="MNPPLRSDNNSPVHLAGYRSAELLPEMDSDRFALLYGRSDGMRE
                     VYSLIDKVAPTDATVFVVGESGCGKELVAHSIHRLSLRATECFVPVNCGAIPANLIEA
                     ELFGFEKGAFTGASRVHKGLFERAHGGTLFLDEITEMAPEMQVRLLRVLESGMFNRVG
                     GHEEIPVDVRVVAATNRDPMQAVDDGHLREDLMYRLAVFPIALPPLRTRGDDILLLAR
                     HFLAEFNAYAACAKTLSDEAMAELRRRPWLGNVRELKNCLQRAFILADDIVGAEHLAP
                     LDGLESERSSLAGRLHFEIGTPLAEMERQTIFATLDHCGGNKKKTAELLGVSLKTLYN
                     RLTEYAAEAPIGLGGGHA"
     gene            complement(3914..6205)
                     /locus_tag="VKZ48_08330"
     CDS             complement(3914..6205)
                     /locus_tag="VKZ48_08330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLU77892.1"
                     /translation="MSDLDFMRHTVAVHAAVPRETAAYARLLEECGYRVLDMAPSEDA
                     WAEADLLLVDEAIATRHGEVLDALKRRDGDRFLPCVLVLPAQADSAQWLDAGFDDVVR
                     LPAQRSEVLARLRVLLRLREHSLEAERLSTARNDMIVASSIDAVIAVDHRGRIVDFNP
                     AAESMFQYPREAVLGESFELLAEERYRARYAESFHRYVVTGRSKIPSRRTELRGRRAS
                     GVRFPAELSLTRVEGRSAPTFYAFVRDLTERKRAERGLAHLAAIVESSEEAIFSQSLN
                     GIITSWNKGAERLFGYTNEEAIGKHADMLLPPGQTRAQSDPAPLAPRRVDVDARRRRK
                     DGSLVDVAVRTSPLLGTDGAVIGVSTIAYDISVRKRERIALQESEQRYRSLVAAMTSV
                     VWTSDGEGRFAVPQSSWEAYTGQQWPAHEGWGWSGMLHPSDRLGIAQRWAAAITARRI
                     YEEGCRVWHARTMSYRHVIMRAVPIRRDDGTVRECIGTLTDVDAAKRAEDAIRALNQE
                     LEQRVIARTADLAEANRELEAFSYSVSHDLRAPLRAIEGFTSILLDDYAPRLDEEGTR
                     LMERVISNVQRMDELVTDLLTFSRMSRVPISKRTVAPGQMVREIVAEFVAASPERRFD
                     VRIGTLPPCSADASLLRQVFVNLIANAFKYSADRDPAVITVDCISDDDPLKPLVYVVR
                     DNGTGFDMRYAAKLFEVFERLHNRQDIEGTGVGLALVRRIVERHGGRVWAESAPQQGA
                     SFYFTLEPDSQADAAALRHSSAA"
     gene            6592..6861
                     /locus_tag="VKZ48_08335"
     CDS             6592..6861
                     /locus_tag="VKZ48_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77893.1"
                     /translation="MDRTDEVNEHVVVRIRQCEASEDGVSTILDLDTDTGPMRLRLDR
                     PFLHTLIGAAAVAPVAQNHALRESADIPPIVRFLTAIQRPLEREK"
     gene            complement(6870..9797)
                     /locus_tag="VKZ48_08340"
     CDS             complement(6870..9797)
                     /locus_tag="VKZ48_08340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HLU77894.1"
                     /translation="MGLRVTRHWPSQILIVGAAIAFVAIEATLAARHIDQDKRQTIED
                     AYTDGEALTLALQAHAGAVFESADAALKGVAAQLEQRGARALGDSAQLNALLAARAHG
                     AAMLHSLLIFLPDGMPLASSNDAHRGLPNLQARESVSVHRAPQVRTGLHVAAPVKNSN
                     GLWLLPLSRAWRDAEGNVQAVLVATVDLDGFRALYDARAVSDVGSSLGLVDAHGRQLV
                     RIPFQEERMGERSRVGTQIVARNAQPAGHLEVWTSTDASRLYNYKWLPGYAVAAYVGQ
                     DRGEVLTAWRTRSSERIRTLGGLSALLLVFAFVAAVKVGRLARDEERLERAAHAASAG
                     IWELKLRNWRMWRSRQWARLLGYEPNEIEATLDGFLSVVHPADYEFARTSMDALRTAT
                     GPVRVEFRVRRKDGAERWVECTAHVFGRRAGDPGYAVGSLRDITRRKGVELSMRESAR
                     MLVLAQRAAEAGVWSVDLKSGAARWSEGCYRLYGFSPVQAPPTIEAWQALVVEEDRER
                     IGRMVRESIVRRVEFSMEYRIRHPEKGLRWLWTLGNTVPDEQGRPAQVAGITLDITER
                     KDAEEERQILLASERKARAEAERASRAKEEFLATVSHELRTPLNAILGWAHVLRTRDK
                     EPDLVLRSVESIERSALAQAQLIADLLDISRIESGTLRLDLEVVDLKAIIDAACNTIL
                     PTAEAKGVRLAWETDSFDVRADPARLQQVMWNLLSNAVKFTPKGGVIKVQTRDAGEMV
                     EIEVCDTGIGIPRDFQPMLFQRFSQSRRSAHQSFGGLGLGLAIVKHLVQAHGGEVQLQ
                     SEGEGKGAVATLRLPMVAARLRVAEPPPESPYVPVALNGVRVLLVEDSVDSREIAALI
                     LSSYGAVVVAVTSVDEALAALDAQLPDLIVADLSMPQRDGFELMRTVRSAADARLRHV
                     PAIALSALASAEDRARAIAAGFNLHLSKPVEPQLLLDAVARLAARSATRPDGTASARY
                     TAA"
     gene            complement(10016..10282)
                     /locus_tag="VKZ48_08345"
     CDS             complement(10016..10282)
                     /locus_tag="VKZ48_08345"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HLU77895.1"
                     /translation="MQWPQAGFTGNRTTDAVNLSIGGMRGERCVSSVRSALSLIRGVQ
                     AVEVSLESGCATVHYNPEKAHPSQFPVAVQAVGFEAALLTPEPA"
     gene            complement(10386..10826)
                     /locus_tag="VKZ48_08350"
     CDS             complement(10386..10826)
                     /locus_tag="VKZ48_08350"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLU77896.1"
                     /translation="MTVRRRIGEGRWERTDTLAYKEEGSAPFKAITRQILFDDPRLQC
                     QLRYFEIAPGGYSTLERHEHMHAVMIVRGRGRCLLGDAVHEVELHDLVTIAPLTWHQL
                     RAAPDEPLGFLCMVNNERDRPQLPTPEQLAELRRNPAVAAFLDG"
     gene            complement(10823..>11400)
                     /locus_tag="VKZ48_08355"
     CDS             complement(10823..>11400)
                     /locus_tag="VKZ48_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HLU77897.1"
                     /translation="CQQAVLRANRETGRNTLYAPSMVGNPASLYAQARLLREEGVGAA
                     LVAPALIGMPVFEELVAAHLQVPVIAHPAYAGAARVAPAFLLGRLFRLFGADAVIYPN
                     YGGRFAYSEAECRAIADNARAPWTGVRSALPVPAGGMTVARVPELVGCYGNEVMLLIG
                     GSLLQAGDALEVRTREFVERVAVCAQQETAP"
BASE COUNT         1989 a         3801 c         3615 g         1995 t
ORIGIN      
        1 acggcgtgcg tttgtccatt caggacaacg ggatcggcat gccggccacc atcgccgccg
       61 acgcgctgtc gcacggcatt ctcggcatgc gggaacgagt gatgggactg ggcggcgtgt
      121 ttcgtatcga aagtccgccc gaaggcggta cgcggatcga ggtgtccgtg cccatgacgc
      181 acgaccctac gacttcgtcg gcgcctaccg atgcgcccgc cgaactgcag tccgtctgaa
      241 ctaaacttta gcggtcgtga tcgcgcaccc tgatacgcgc ataacggcga tcgtcggaag
      301 gcggctcgaa gggcgcaccc gtcaaaatcg taaaaataaa catcaacagc atgatccggc
      361 ctcttgtcgc gagcgcttcg ccggctgatg cggcgcgctg gatgatccca gcatagaccg
      421 tttcggatgc cggtctttga tcgatatcat cgcccgtcgc ttccgccgcc ctggggcatc
      481 cgccgtcgtc agggcacggc gcgcatcggt gcgtgcggcc tcataatccg cgcgcgcgct
      541 tctcagacag acatagtcag ttcggcgcgg aattgttcgc ggctcatggc accgcacgag
      601 acactcaacg caaccgtcac aggatgcatc ggatcggcct catgcggcac cctgtgaatc
      661 agataccgcg ctcccagtcg ctgacctgct tgtcggcctc ttcgcgcgaa atgccgtacg
      721 cctcctgaat cttgccgctc aattgctcgc gcttgccttc gatcatgtca agctgatcat
      781 cggtgagttt gccccattgc tccttcactt tgccgcgcag ttgcttccag tttccttcga
      841 tacggtcctt gttcatacgt ctctcctaga gtgtgcgcag gatgcgctca gacttggcga
      901 cgcaaccggc atgcccgtga cgagagctgc gcaccaacaa ataaaacggc cgcccggagg
      961 cggccgcttg gtgtggtgtg tatcgaagct atcttcgcaa caacccggtg atgagcgaga
     1021 ccaggaacag caccaggaag acgaagaaca ggattttcgc aatctccgcc gcgccggcag
     1081 cgatcccgcc aaagccgaat atcgcggcaa tgattgcgat gatgaagaat gcgatcgccc
     1141 aattcagcat gagtcgctcc tttattgaga cgggcactcg cccgataccg tgaccggggc
     1201 agcagccgcg cgatgcatct cagcttgcaa gtcgcgtacc tctggcgccc gacggcggtg
     1261 cacgttactt gttgcgatca gccgcgtttt ccagtttctc gccgccgcgc tggatatcct
     1321 ggccggcacc ttccatggtg ttacatccga taagcgcgaa cattacgagc agtagtgcag
     1381 atacggcttt caacatggtc atctccttta cagtccgagg tttacggacg cctgatgcgt
     1441 ccctgtcttc tgcactgcaa cacccgtgcc aaaatggcta agacgcgata tgttttgctg
     1501 cacacgcttc ttcgcgtgcg cgcgccatgt cttcgagtac ttcgcgcacg cgtgcatgct
     1561 cgcgcgcttt gtcgaagaag aacgacgcac ccaactgcgc gctcttctcg cggtaatgcg
     1621 ggaagtcgta gtttgtaaag acgatgtaca ccggctcgcc cggccgggcg cgcgccgatt
     1681 tgagtacgcc gagcccgctt ccctgcttga gttgcaaatc gaggacgacg gcgtcgcagg
     1741 gcgtgtcgcg caaggcgcgc aaggcatcgc cttcgcagtc ggcctgcccg acgatctcga
     1801 tccggccggg ctcgacgatc gactcggcca accgttcgcg gatgatggcc gagtcctcga
     1861 tcagcagtac ccgtacgggc agcgaccggg gtgctgcgct gacctgggac ccaccgttct
     1921 tgccgttcat tggatgagct gattcttgat cgcgtagtag gtcaggtccg cgttggtctt
     1981 catgcccatc ttttccagga tgcgcgtgcg gtaggtgctg accgtcttga ctgacaagaa
     2041 cagttcctcg gcgatttttg acaccgcctg cccggcggcg agcttgcaga aaatctggaa
     2101 ctcgcgctcc gacagcgcag tgtgcaacgg ccgctcgctt gccgggctgc tcacatcgtg
     2161 tgcgaggatt tccgccagtg tgctgctgac atagcgccgg cctgcgacca cggtgcgaat
     2221 cgcgcgcacc agttcggcgg gcgcgctttc cttgttgagg tagccgcttg cgccggcgcg
     2281 cagcagattc accgcgtact gcccttccgg aaagccggag aggatcagca caggcagttc
     2341 cggcttgacg tgcttgaggc tcttgagcgt atccacaccc gatcggtccg gcatggagat
     2401 gtcgagcagc accacatccc attcgccgtt gcgtaccaga tcgagcgtct gcgagccggt
     2461 ttccgcttcg cccgtcaccg ccatgtcggg ctcgtcggcg atgaactgct tgaggccggc
     2521 gcgcacgatg gcgtgatcgt ccgcgatcag gatgcgaatc cggctgggcg gtatacgtgt
     2581 ggtggcctgc ataaagcgcg ctgccgatgc tcccgcttgt agtacggctt cacgcatggc
     2641 cgcctcccaa gccgatggga gcctccgcgg cgtactcggt gagccggttg tacagggtct
     2701 tcaggctgac gccgagcagt tcggcggtct ttttcttgtt gccgccgcag tgatcgagcg
     2761 tcgcgaagat cgtctgccgc tccatctcag caaggggagt gccgatttcg aaatgcagcc
     2821 tgccggccag gctgctccgt tcgctttcca acccgtccag gggagccagg tgctcggcgc
     2881 cgacaatatc gtcggccagg atgaaggcgc gctgcaggca gttcttcaac tcgcgcacat
     2941 tgccgagcca ggggcgtcgg cgcagttccg ccatcgcctc atccgacagg gtcttcgcgc
     3001 aggcggcgta ggcattgaat tcggccagaa aatggcgcgc cagcagcagg atgtcgtcgc
     3061 cgcgcgtacg cagggggggc agtgcaatgg gaaagaccgc caggcggtac atcaggtctt
     3121 cgcgcaaatg gccgtcgtcc accgcctgca tcggatcgcg attggtcgcc gcaacgacgc
     3181 gcacgtcgac cggaatctcc tcgtgccctc cgacgcggtt gaacatgccg ctttccagca
     3241 cgcgcagcag gcgcacctgc atctcgggcg ccatctcggt gatctcatcc agaaacagcg
     3301 tcccgccgtg tgcgcgctcg aacagaccct tgtgcacgcg tgaagcgccg gtgaaagcgc
     3361 ccttctcgaa accgaacagt tccgcctcga tgagattggc aggaatggcg ccgcaattga
     3421 ccgggacaaa gcattccgtg gcacggaggc tcaaccgatg gatgctatgc gccaccagtt
     3481 ccttgccgca cccgctttcg cctacgacaa acaccgtcgc gtccgtcggt gccaccttgt
     3541 cgatcaggct gtacacctcg cgcatgccgt ccgagcggcc gtacagcagg gcgaaacggt
     3601 cgctgtccat ctcgggcagc agttcggcgg aacgataacc ggccagatgg acaggactgt
     3661 tgttgtcact gcgtaggggc ggattcatgg tctcctttcc acaatcggcg cagtccgttt
     3721 ctcaggatcg cgtctatgca gcgttgaccc ctgcaaagcg cacgcagggg aacacccgta
     3781 tggcgttcgc ctaggataga aatccccacc gcaatactgt atcggtccgt cccccaacca
     3841 cacgtcggat tcctcctacg ccgcaggcgg acaaaaattg acgaaccgta aggactgtaa
     3901 gaagtgcgtc cgctcaggcg gcggacgagt gccgcagtgc tgccgcgtcg gcctgcgaat
     3961 ccggttcgag cgtgaagtag aagctcgcgc cctgctgcgg cgcggattcc gcccagaccc
     4021 ggccgccatg gcgctcgacg atgcgccgta ccagcgccag acccacgccg gtgccttcga
     4081 tgtcctggcg gttgtgcaac cgctcgaaca cttcgaacag tttcgcagcg tagcgcatat
     4141 cgaatccggt gccgttgtcg cgcacgacgt ataccagcgg cttgagcgga tcgtcgtcgc
     4201 tgatgcagtc gacggtaatg accgcggggt cgcggtccgc gctgtacttg aaggcgttgg
     4261 cgatcagatt gacgaacacc tggcgcaaca gggaagcatc ggccgaacac ggcggcagcg
     4321 tgccgatgcg cacgtcgaag cgccgctcgg gcgaggcggc cacgaattcc gcgacaatct
     4381 cgcgcaccat ctgtcccggc gcgaccgtgc gcttgctgat cggtacgcgg ctcatacggg
     4441 aaaaggtcaa aagatcggtc accagttcgt ccatgcgctg gacgttggaa atgacgcgct
     4501 ccatcaaccg cgtgccctct tcgtcgagtc gaggggcgta gtcgtcgagc aggatggagg
     4561 tgaagccttc gatggcgcgc agcggcgcgc gcaaatcatg cgaaacggaa taggagaatg
     4621 cctcgagttc gcgattggcc tcggccaggt cggcggttcg cgcaatgacg cgctgctcca
     4681 gttcctgatt gagcgcgcgt atcgcatcct ctgcgcgctt cgccgcatcc acatccgtga
     4741 gcgtaccgat gcactcgcgc accgtgccgt cgtcgcgccg gatcggcacc gcgcgcatga
     4801 tgacgtgccg atagctcatg gtgcgcgcgt gccacacgcg gcaaccctct tcgtaaatgc
     4861 gccgcgccgt aatggccgcc gcccagcgct gcgcaatgcc gaggcggtcc gacggatgca
     4921 gcatgcccga ccagccccac ccctcgtgtg ccggccactg ctggccggta tacgcctccc
     4981 aggaggactg cggcacagcg aagcgtcctt cgccgtcgct ggtccatacg accgaagtca
     5041 ttgccgcgac cagggaccgg tagcgttgtt cgctttcctg cagcgcgatg cgctcgcgct
     5101 tgcgcacgct gatgtcgtag gcaatggtgg acacgccgat cacggcgccg tcggtaccga
     5161 gcaacggcga ggttcgcact gcgacgtcga ccaggctgcc atccttgcgc cgacgccgcg
     5221 catcgacgtc gacgcggcgc ggcgccaatg gcgcaggatc ggactgcgcc cgggtctgtc
     5281 cgggcggcag cagcatgtcg gcgtgcttgc cgatcgcctc ctcgttcgtg tacccgaaca
     5341 ggcgttccgc gcccttgttc caactggtga tgatgccgtt gaggctttga ctgaagatgg
     5401 cctcttcgga gctttccacg atcgccgcca gatgcgcgag cccgcgctcg gcgcgcttgc
     5461 gctcggtgag atcgcggacg aaggcgtaga aggtcggcgc cgaacggcct tcgacgcgcg
     5521 tcaggctcaa ctcggcggga aagcgaacgc cgctggcacg ccggccgcgc aactcggtgc
     5581 gccggctggg aatcttgctg cggccggtga cgacatagcg gtgaaagctc tccgcatagc
     5641 gcgcgcggta ccgctcctcg gccagcagtt cgaaggattc gccgagcacc gcctcgcgcg
     5701 gatactggaa catgctttca gccgcagggt tgaagtcgac aatccggccg cgatggtcga
     5761 ccgcgatcac cgcgtcgatg gacgaagcga cgatcatgtc gttgcgcgca gtgctcagcc
     5821 gctctgcttc cagcgaatgc tcgcgcagac gcagcagcac gcgcagacgc gccagcacct
     5881 cgctgcgctg cgcgggcagc cgcaccacgt cgtcgaagcc cgcatcgagc cactgcgcac
     5941 tatctgcctg cgccggaagc acgagcacgc agggcaggaa gcggtcgccg tcgcgacgct
     6001 tgagcgcatc cagcacctcg ccatgccggg tggcgatcgc ctcgtcgacc agcagcagat
     6061 ccgcttccgc ccaggcgtcc tccgacggcg ccatgtccag aacgcggtag ccgcattcct
     6121 ccagcagacg cgcataggcc gccgtctcgc gcggcacggc cgcgtggacc gccacggtat
     6181 gacgcatgaa gtcgagatcg ctcatcggaa tgcgacggcg gtcaggcacg gcgacgcggc
     6241 gccggccatg gaacggcggg cgcatgtgcg cgcggaacgg caggggtgaa acaggctcaa
     6301 gactcgatcc gactgctaac ctgcgcgagc ggacggatcg ctgtgggcgt accgctctat
     6361 gcgtctcgcg cggggaggaa acagggttcg ttatggatgg cgatcggcca agtcttgtta
     6421 ttcgaacagc gcggcgggcc accggggcgc gccgaatgac gggaatatag caaatcccgg
     6481 ggtcgagaca gcgcggtaaa tcccggcaaa cgcgacaaaa ggcgctccgc accgcgagtc
     6541 gggcatcggg cttgcaaggg aaggagcagc gcaattccca ggcagcaccc catggaccgt
     6601 accgacgaag tcaatgagca cgtggtggtg cgaatcaggc aatgcgaggc gtcggaggat
     6661 ggggtttcca ccattctgga tctcgacacc gacacggggc cgatgcggct ccgcctcgac
     6721 cggccttttc tgcacacgct gatcggcgcg gcggccgtcg caccggtggc gcagaaccac
     6781 gcgctgcgcg agtcggccga catcccgccc attgtgcgtt ttctgaccgc catccagcga
     6841 ccgctggaac gcgagaaata agcgcagctt cacgcagccg tatagcgcgc cgacgcagta
     6901 ccgtccgggc gtgtcgccga tcgcgcggcc aggcgcgcca ccgcatccag cagcagttgc
     6961 ggctcgaccg gcttggacaa atgcaggttg aagcccgcgg cgatggcccg cgcccggtct
     7021 tccgcgcttg cgagcgcgga gagcgcaatg gcgggtacat gacgcaagcg cgcatcggcc
     7081 gcactgcgca cagtgcgcat gagttcaaac ccatcgcgct gcggcatact cagatcggcc
     7141 acgatgagat cgggcagttg tgcatcgagt gccgcgagcg cttcgtccac cgacgtcacc
     7201 gccaccacga ccgcgccata gctgctcagg atcagtgcgg caatttcacg cgaatcgacg
     7261 ctatcttcca ccagcagtac gcgcacgccg tttaaagcga ccggcacata gggcgattca
     7321 ggcggcggtt ccgcgacgcg caatcgcgct gcaaccatag gcaggcgcag ggtcgcgacg
     7381 gcgcccttgc cttccccctc gctttgcaac tgcacttctc cgccgtgcgc ctgaaccaga
     7441 tgcttgacga tcgccaggcc cagtcccagc ccgccgaagg attggtgcgc gctgcgacgc
     7501 gactgactga agcgctggaa cagcatgggc tggaaatcgc gcggaatgcc gatgcccgtg
     7561 tcgcatactt cgatttcgac catctcgccc gcgtcccgcg tctgcacctt gatgacgccg
     7621 cccttcggcg tgaacttcac tgcgttggaa agcaggttcc acatcacctg ctgcaatcgg
     7681 gcggggtcgg cgcgcacgtc gaaggaatcc gtttcccagg ccagtcgtac gcccttggct
     7741 tcggccgtgg gcaggatggt gttgcatgcg gcatcgatga tcgccttcag atcgaccact
     7801 tccaggtcga gccgcagcgt gccggactcg atgcggctga tatccagcag atcggcaatg
     7861 agttgcgcct gggccagcgc gctgcgttcg atggactcga cgctgcgcaa caccaggtcg
     7921 ggctccttgt cgcgcgtgcg cagcacatgc gcccagccga gaatcgcatt gaggggcgtg
     7981 cgcagctcgt gcgataccgt ggcaaggaat tcctccttgg cccggctggc gcgctccgcc
     8041 tcggcgcgcg ccttgcgttc gctcgccagc aggatctgac gctcctcctc cgcatccttg
     8101 cgctcggtga tgtccagggt aatgcccgcg acctgcgccg gccgtccctg ttcgtccggc
     8161 accgtgttgc ccagcgtcca caaccagcgc aggccttttt caggatgccg gatgcggtac
     8221 tccatcgaga attccacgcg gcgcacgatg ctctcccgca ccatgcgccc aatgcgttcg
     8281 cggtcttcct cgaccaccag cgcctgccac gcttcgatgg tcggcggcgc ctgcaccggg
     8341 ctgaacccgt agagtcggta gcagccttcc gaccagcgtg ccgcaccgga cttcaaatcc
     8401 acgctccaca cgcccgcctc cgcggcacgc tgcgccagca ccagcatgcg cgcgctttcg
     8461 cgcatcgata gctcgacgcc tttgcgacgg gtgatgtcgc gcaatgagcc gaccgcgtag
     8521 ccgggatcgc ccgcacggcg accgaacacg tgcgcagtgc attcgaccca ccgttcggcg
     8581 ccgtccttgc gccgcacccg gaactcgaca cgcacggggc cggtagcggt gcgcagcgcg
     8641 tccatcgagg tacgcgcaaa ctcgtaatcg gccggatgca cgaccgacag aaagccgtcg
     8701 agcgtcgctt cgatttcgtt cggttcgtaa ccgagcagcc gcgcccattg ccgcgaacgc
     8761 cacatgcgcc agttgcgcag cttcaattcc cagatgccgg ccgaggcagc gtgcgccgcg
     8821 cgctccagac gctcttcgtc gcgcgccagc cgcccgacct tgacggcggc cacgaaggcg
     8881 aaaaccagca atagcgccga caagccgccc agcgtgcgga tgcgttcgct gctgcgcgtg
     8941 cgccatgccg tcaggacttc gccgcggtcc tgacccacat aagcggccac cgcgtagccg
     9001 ggcagccact tgtagttgta gagacggctc gcatcggtgc tcgtccagac ttcgaggtgc
     9061 cctgcgggtt gtgcattgcg cgcgacgatc tgcgtgccga cgcggctacg ctcgcccatg
     9121 cgctcttcct ggaacgggat acgcaccagt tggcgtccgt gcgcatccac caacccgagc
     9181 gaggagccga cgtccgagac ggcgcgcgcg tcgtacagcg cacggaaacc gtcgagatcc
     9241 accgtggcga ccagcaccgc ctgcacattg ccttccgcat cgcgccatgc gcgactgagg
     9301 ggcaacagcc acagaccgtt gctgttcttg actggcgcgg cgacatgcag tccggtgcgg
     9361 acttgcggcg cacgatgcac gcttacggat tcgcgcgcct gcagattagg caggccgcga
     9421 tgtgcgtcgt tgctgctggc cagcggcatc ccatccggca ggaagatcag cagcgaatgg
     9481 agcatggccg caccgtgtgc gcgggccgcc agcagggcgt tcagttgcgc actatcgccc
     9541 agggcacgcg cgcctcgctg ctcgagttgc gccgcgacgc ccttcaatgc ggcatccgcg
     9601 ctttcgaaca cggcgccggc atgcgcctgc aatgcgaggg tgagcgcttc accgtcggta
     9661 taggcgtctt cgatggtctg acgcttgtcc tgatcgatgt gacgcgcggc cagtgtggct
     9721 tcgatcgcga caaaggcaat ggcagcgccg acgatgagta tctgcgacgg ccagtgacgc
     9781 gtcactcgca aacccatgtc cgtcccctcg ggcgaccctt ggacggccgc cggttgcccc
     9841 accgcaccgg cgggggatcg aggcgtccag cgcaaaacca cctcggtgag cggctatttt
     9901 tagacgcctt gcggcgcgga tgcaagcgcg tttttaccgg cagcagtcga tccggccggg
     9961 ggaaaagaca aggccctcac ggggagggcc ttgtgcagtc agccttcaag gcgattcaag
    10021 ccggctcggg cgtcagcagt gccgcctcga acccgacggc ctggaccgct acgggaaatt
    10081 gcgacggatg cgccttctcg gggttgtaat gaacggtcgc gcagcccgat tccagcgata
    10141 cctccacggc ctggacaccg cgaatgaggg acagggcgct gcgcacactg gagacgcaac
    10201 gctcgccgcg cattcccccg atactcaggt tcaccgcgtc cgtggtccgg ttgccggtga
    10261 accctgcctg tggccattgc atgatttgct ccttgtgacg tttcgtgaca ttcgttgcaa
    10321 gccccactgg gagtgcaacg gctatgcctg tcactcggga caatgcgccg ccgggcggag
    10381 ggagatcagc cgtcgagaaa ggcggcgacg gccgggttgc ggcgcagttc ggccaactgt
    10441 tctggagtgg gcaactgcgg ccggtcgcgc tcgttgttca ccatgcagag gaagccgagc
    10501 ggctcgtcgg gggcggcacg caactgatgc caggtcagcg gtgcgatggt caccagatcg
    10561 tgcagctcga cttcatggac tgcatccccc agcaggcagc ggccgcggcc gcgcacgatc
    10621 atcacggcat gcatgtgttc gtgccgctcc agggtggaat agccgcctgg cgcgatttcg
    10681 aaatagcgta gctggcattg caggcgcggg tcgtcgaaca ggatctggcg cgtgatcgcc
    10741 ttgaacggcg cactgccctc ctctttgtag gcgagcgtgt ccgtccgttc ccaccgccct
    10801 tcgccgatgc gtcgtcgcac tgtcatggcg cagtctcctg ttgcgcgcag acggccacac
    10861 gctcgacgaa ttcgcgcgta cgcacctcca gcgcatcgcc tgcctgcagc agcgagccgc
    10921 cgatgagcag catcacctcg ttaccgtagc acccgaccag ttccggcacg cgcgcgacag
    10981 tcatgccgcc ggcgggcacg ggcagcgcgg agcggacgcc cgtccagggc gcgcgcgcgt
    11041 tgtcggcgat cgcgcggcac tcggcctcgc tgtaggcgaa gcggccgccg tagttcggat
    11101 agatcaccgc gtccgcgccg aataggcgga acaaccggcc gagcaggaac gcgggcgcga
    11161 cccgcgccgc gccggcgtag gccgggtgcg caatcacggg aacctgcaga tgcgcggcaa
    11221 ccagttcctc gaacaccggc atgccgatca gcgccggcgc gacgagcgcg gcaccgacgc
    11281 cctcctcgcg cagcaggcgc gcctgtgcat acagcgatgc ggggttgccg accatgctag
    11341 gcgcatagag cgtgttgcgc ccggtctcgc gattggcacg cagcaccgcc tgctggcagg
//
LOCUS       DATGOU010000146         4431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_1324646, whole genome shotgun sequence.
ACCESSION   DATGOU010000146 DATGOU010000000
VERSION     DATGOU010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 4431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4431
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324646"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="VKZ48_08360"
     CDS             complement(<1..1015)
                     /locus_tag="VKZ48_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77898.1"
                     /translation="MNLNLFDHKGTPLDKQRFTWREIAGHKPISKLDDDAFTRIRVIL
                     MNGVESDAVRLQHTMMRFNRELRLPLATMMRTEHHQQTLVNWLLSADHSPLETTIGYE
                     QVAIEVTSSVAQNEPDAYLSQVYRFGLLEDFDHLYRYSALMDRLEGKDANNILQGYTD
                     IIPGRPTVIEHRHPEDDLRDPYGPDADPLSKIHATLITAAEYQTHDYYMHFGPTFADP
                     VARQIYAEIASVEAQHITQYGSLIDPGDEGITQWLMHEAMEAYTYWCCMEQETNPRIK
                     ATWERFFDYEMGHFHMVADIYKNALRRDPQEFFPKAFDKRIEFVSHRDYVKQVLQDEV
                     DLRA"
     gene            complement(1054..1623)
                     /locus_tag="VKZ48_08365"
     CDS             complement(1054..1623)
                     /locus_tag="VKZ48_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77899.1"
                     /translation="MKAGKRNRTQQLGAALIVTAFALPVLAQTGGTTGTAGGTTGSAV
                     GGPDAGTATMPGSTNSGMTTGDAPASGPLGSGPRDARDIGTPPRDARDINGTGVRIDR
                     PDAAGSAATNSSGSVGGAGSMTPDAQSDALESGTDPRGTRGAMRGMDRRGDPDWRMEH
                     DRQMDAEQRRRMLERQRGTTVPPGPGSYR"
     gene            complement(1757..2197)
                     /locus_tag="VKZ48_08370"
     CDS             complement(1757..2197)
                     /locus_tag="VKZ48_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77900.1"
                     /translation="MKFLRNATLAAGVAALFAAGPALANETDSSVQSAPQQLGGPAAD
                     SQANSQANQSQSDRASRAESAERYGSQSGASGSASGTMSGSTGSQGRYGTEPGTSAGQ
                     GSAVGSEGGGASGSGDFQGSTELGSPATESLNQPSSGSPSSTSQ"
     gene            complement(2360..3676)
                     /locus_tag="VKZ48_08375"
     CDS             complement(2360..3676)
                     /locus_tag="VKZ48_08375"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HLU77901.1"
                     /translation="MVERYRQDDEWRNRMEQGARDDRSQERYASQGDWRNQQRPYRQD
                     EQSSYGRSGQQSQQGYGQRGFDERRPQWQQEQRGGLHEHYGGIRGHGGFMGHGSPDER
                     YGQQGGYGVDVYQDREHEIPTYGSGRGPYASLAGYGAYSGAGPDNYNVGPREHEGSWH
                     GGDVHNPPNWGQRPSYGPYGGAPSQRDYGAYGAQGYGGEGGSGAQGYGGQGAYGRYGS
                     ESDYGTPGGYGGDRVYRQGGARHDTGGGAHYGALVSYGQGAYGGYGQGEMGRSSGQGG
                     QSWGNWGQSGQPGRSQRRGPKNYQRSDERLQEEICERLMARWDLDTSEVSVQVSQGEV
                     TLEGVVPERRMRHEIENLVDDCHGVKDIDNRIRVQSAYGSTSSGSSAEHQFSQSGSSR
                     QSSSGQQSQASVTASQPGQDSSKDARMGSTAPSSAPARSASDIKKE"
     gene            complement(3867..4295)
                     /locus_tag="VKZ48_08380"
     CDS             complement(3867..4295)
                     /locus_tag="VKZ48_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004523329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLU77902.1"
                     /translation="MQVSEAMTRDVRVASPTDTLRDVARIMADIDAGAVPIGDNDRLV
                     GMITDRDIAVRAVAMGKGPDTQVQEIMSSEVLYCYEDDDIDDVAENMADVQVRRLPVV
                     NRDKRLVGIIALADIAEREGPHAAGVAVEGISKPGGPHSQ"
BASE COUNT          676 a         1497 c         1394 g          864 t
ORIGIN      
        1 acgcgcgcag atcgacttcg tcctgcaaca cttgcttgac gtaatcgcga tgactgacga
       61 actcaatccg cttgtcgaag gccttgggaa agaactcctg cgggtcgcgg cgcagcgcgt
      121 tcttgtagat atccgccacc atgtgaaagt gtcccatctc gtaatcgaag aaacgctccc
      181 aggtcgcctt gatgcgggga ttggtttcct gctccatgca gcaccagtag gtgtaggcct
      241 ccatcgcctc gtgcatcagc cactgcgtga tgccctcgtc gccgggatcg atgagcgagc
      301 cgtactgggt gatgtgctgc gcctcgaccg aggcgatttc ggcgtagatc tggcgcgcca
      361 ccggatcggc gaaggtcggc ccgaagtgca tgtagtagtc gtgcgtctgg tactccgccg
      421 cggtgatgag tgtcgcgtgg atcttgctca gcggatcggc gtccggaccg tagggatcgc
      481 gcagatcgtc ctccgggtgc cggtgttcga tgaccgtcgg gcgccccggg atgatgtcgg
      541 tgtagccctg cagaatgttg ttcgcatcct tgccttccag acggtccatc agcgccgaat
      601 agcggtagag atggtcgaag tcctccagca ggccgaagcg gtacacctgt gagagatagg
      661 cgtcgggctc gttctgcgcg accgaggagg tgacctcgat cgccacctgc tcgtagccga
      721 tcgtcgtctc cagcggcgaa tgatccgcgc tcaacagcca gttcaccagc gtctgctgat
      781 gatgctccgt gcgcatcatg gtcgccagcg gcagccgcag ttcgcggttg aaccgcatca
      841 tggtgtgctg cagacgcacg gcgtccgatt cgacgccgtt catgagaatg acgcggatgc
      901 gggtaaaggc atcgtcatcc agtttgctga tgggcttgtg tcccgcaatc tcgcgccagg
      961 tgaagcgctg cttgtcgagg ggcgtgccct tgtggtcgaa aagattcaag ttcatagctg
     1021 ttccgcgtca aagaagtgaa aaccgccgtg cgctcagcgg tacgagccgg gacccggcgg
     1081 caccgtcgta ccgcgctggc gctccagcat gcggcgccgc tgctcggcat ccatctgcct
     1141 gtcgtgctcc atgcgccagt ccggatcgcc gcgacggtcc atgccgcgca tcgcgccgcg
     1201 cgtaccgcgc ggatcggtgc ccgactcgag cgcatcgctt tgcgcatcgg gcgtcatcga
     1261 acccgcgccg cccacgctgc cggaggaatt cgtggctgcg ctgcccgccg catcggggcg
     1321 atcgatccgc acgccggtcc cgttaatgtc gcgtgcatcg cgcggcggcg tgccgatatc
     1381 gcgtgcgtcg cgcgggccgc tgccgagcgg gccgctggcc ggagcgtcgc cggtggtcat
     1441 gccgctgttc gtgctaccgg gcatggtcgc cgtccctgcg tccggtccgc cgaccgccga
     1501 gccggtggtg ccacccgccg tgcccgtcgt gccaccggtc tgcgccagca ccggtaatgc
     1561 gaacgccgta acgatcagcg cagcgcccaa ctgctgcgtc ctgttgcgct tgcctgcttt
     1621 catgtcgatc tccttatgtg gacccgctcg gtgaacgtgc tcaacgcggg caatgcccat
     1681 gccttgcgtc cggaggcgat gtaaaaggcc ggcgcgacgg ccggcctttg catgacagcg
     1741 caacgaccga catcggtcat tgggacgtgg acgacggcga gccggatgaa ggctggttca
     1801 gcgattcggt cgcagggctg cccagttcgg tcgagccttg gaaatcgccc gaaccggatg
     1861 cgccgccgcc ttcgctgccg acggcgctgc cctgaccggc agacgtgccg ggctccgtgc
     1921 cgtaccggcc ctgactgccg gtgctgccgg acatggtgcc gctggcgctg cccgatgcgc
     1981 ccgactgcga gccgtagcgt tcggccgatt ccgcgcgcga agcacgatcg ctctggctct
     2041 ggttcgcctg gctgttggct tggctgtcgg ccgcgggacc gcccagttgc tgcggcgcac
     2101 tttgcacgct gctgtccgtc tcgttggcca gtgcaggtcc tgcggcgaat agcgcggcca
     2161 caccggccgc gagtgttgcg ttgcgtaaga atttcatcgc gtgtctccgt atggaaaggg
     2221 gatgcgcgac ttcatggaag ccaccgccct tgagtcgcaa cgcgcatgcc ggtaagcgct
     2281 accgcgaagc ggttgtccgc ccctgtccga catgaaaaag gccggcacat ggccggcctt
     2341 cgcccagctt acaaaagcgt cactccttct tgatgtcgga cgccgacctt gccggcgcac
     2401 tggacggcgc ggtggaaccc atccgtgcat ccttggacga gtcctgaccc ggctgggaag
     2461 cggtcaccga ggcttgcgac tgttggccgc tgctcgactg tcgggaactg ccggattgag
     2521 agaactgatg ctcggcggat gaaccggacg aggtgctgcc gtaagcgctt tgcacgcgta
     2581 tgcgattgtc gatgtccttc acgccatggc aatcatcgac caggttttcg atttcatgcc
     2641 gcatacgccg ctcgggcacg acgccttcga gcgtaacttc gccttgcgag acctggaccg
     2701 acacttcgct ggtatccaga tcccaacgcg ccatcaggcg ttcgcagatc tcttcctgca
     2761 ggcgctcgtc cgagcgttgg tagttcttgg gaccgcggcg ctgcgaacgg ccgggttggc
     2821 cggactgccc ccagttaccc cagctttgtc cgccttgccc gctggaccgg cccatctcgc
     2881 cctgaccata accgccatag gcgccctgac cgtaactcac gagcgcgccg tagtgcgcgc
     2941 cgccgcccgt gtcatgccgt gcgccgccct ggcgatagac gcgatcgccg ccatagccgc
     3001 cgggcgtgcc gtagtcgctc tccgagccgt agcgcccgta tgcaccctgc ccaccgtagc
     3061 cttgcgcacc cgacccgcct tcgccgccgt atccctgtgc gccgtaggcg ccatagtcgc
     3121 gctgcgacgg ggcgccgcca taagggccgt acgacggacg ctgcccccag ttcggtggat
     3181 tgtgcacatc gccgccgtgc cagctgcctt cgtgttcacg cggccccacg ttgtagttat
     3241 ccggcccggc gccgctgtaa gcgccgtagc ccgcaaggct ggcgtaaggt ccgcgccccg
     3301 agccgtaggt cggaatttca tgttcgcggt cctgatacac atcgacgccg taaccgccct
     3361 gctgaccgta acgctcatcc ggagagccgt gccccatgaa cccgccatgg ccgcgtatgc
     3421 caccgtagtg ctcatgcaaa ccgccacgct gctcctgctg ccattgcggc cgacgttcgt
     3481 cgaaaccgcg ctgaccgtaa ccctgctgcg actgctgacc gctgcggcca tagctgctct
     3541 gctcatcctg gcgatagggg cgttgctgat tgcgccaatc gccttgcgat gcgtatcgct
     3601 cctgcgaccg gtcgtcgcgt gcgccttgtt ccatccggtt ccgccattcg tcgtcctggc
     3661 gatatcgttc aaccattgct cactccttcg atgcgttgca agccgatggc gggcaaactg
     3721 cccgcgggct cgtgagaggc ggcgcgcacg ccacccgtat gtgaaggttg agcaagccgc
     3781 gtaccccgca gcgataaaac ggcgcgagct gtcgaagacg ggcggccgca tgccgcccgt
     3841 cgagtggaat gattgcgagt gtcgagttat tgcgagtgcg gtccgcccgg tttggaaatg
     3901 ccttcaaccg cgacgcccgc cgcatgcggc ccttcgcgct cggcgatatc ggccagcgca
     3961 ataatgccga ccagccgctt gtcgcgattg acgaccggca ggcgacgcac ctggacgtcc
     4021 gccatgttct ccgccacgtc gtcgatgtca tcatcttcgt agcagtacag cacctcgctg
     4081 ctcatgattt cctgcacctg cgtgtccggg cccttgccca tcgccacggc ccgcaccgcg
     4141 atatcgcgat cggtgatcat gccgaccagt cgatcgttgt cgccgatcgg caccgcaccg
     4201 gcatcgatgt cggccatgat ccgcgccaca tcgcgcagtg tgtcggtcgg gcttgccacg
     4261 cgcacatcgc gtgtcattgc ttcgctgact tgcattgcgt tctcctttaa gaaatcgcgt
     4321 ccggcgcttg ccggactccg cactgcatgc attcaagcgc cgtgcgcatc acccgtgccg
     4381 cccacatgtg cgatgtgcag aaactcgcgg cgcgtatcgc tgttggtgcg g
//
LOCUS       DATGOU010000147         9334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_877587, whole genome shotgun sequence.
ACCESSION   DATGOU010000147 DATGOU010000000
VERSION     DATGOU010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9334
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877587"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VKZ48_08385"
     CDS             <1..293
                     /locus_tag="VKZ48_08385"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006754984.1"
                     /note="catalyzes the oxidation of malate to oxaloacetate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HLU77903.1"
                     /translation="IEARGSSSAASAASAAIDHMRDWVLGSNGEWVTMGVPSDGSYGI
                     PEGIVYGFPVICADGGYEIVRDLDVDDFSRQKMDATLKELLEERDGIKHLLS"
     gene            391..3093
                     /gene="acnA"
                     /locus_tag="VKZ48_08390"
     CDS             391..3093
                     /gene="acnA"
                     /locus_tag="VKZ48_08390"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003264018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HLU77904.1"
                     /translation="MPHNPFDTLQEFKPGKGKTGRFYSLPTLQRTGFPNLSRLPVSIR
                     IVLEAVLRNVDGQKVTADHVRQLAKWQPNAARTEEIPFVVARIVLQDFTGVPLLADLA
                     AMRGVADRMGKDPKLIEPLVPVDLVVDHSVQVDSYGNPNALDINQKLEFKRNRERYQF
                     MKWGMQAFDTFKVVPPGIGIVHQVNLEYLARGVHQQDGLYYPDTLVGTDSHTTMINGI
                     GVVGWGVGGIEAEAGMLGQPVYFLTPDVVGVHLKGSLRGGVTATDLVLTITEMLRKAK
                     VVGKFVEFFGEGVENLSVPDRATIGNMAPEYGATIGFFPVDDKTVAYFEDTGRTKEEI
                     EAFRAYFKAQELYGIPKLGDIDYSEVLELDLSKVAPSLSGPKRPQDRIEIGDVKAQFT
                     KLFSAPTSQNGFAKKAEDLPKRYTTKDGIDIGSGDVLIAAITSCTNTSNPGVLLAAGL
                     LAKKAVERGMTVKPHIKTSLAPGSRVVTEYLRKAKLLPYLEKLGFYLAGYGCTTCIGN
                     SGPLAEPIEEAIVDHDLVCAAVLSGNRNFEARIHSNIRANFLASPPMVVAYALAGTML
                     KDLMTEPVGKGKDGEDVWIGDIWPTSEEIYECMKHAKNPSTFRKLYTDLAEQNPLWKE
                     IGATAGQVYDWPPSTYIAEPPFFEGFGMQPGRIGDVHGARILALFGDSITTDHISPAG
                     AIQETSPAGQYLIKHGVLKPDFNSYGARRGNHEVMMRGTFANVRIKNLMLPPKEDGSR
                     VEGGYTLFQPSGERMAIYDAAMRYIESGTPTVVFAGEEYGSGSSRDWAAKGTQLLGVK
                     AVIARSFERIHRSNLVGMGVLPCQFIGNDSVETLGLKGDEVIDLLGVEGELRPQQELT
                     LIIKSANGEQRSIKVLLRIDTPIEVDYYRHGGILPFVLRELLAA"
     gene            complement(3205..3414)
                     /locus_tag="VKZ48_08395"
     CDS             complement(3205..3414)
                     /locus_tag="VKZ48_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HLU77905.1"
                     /translation="MASGIVKWFNDQKGYGFITPDGGGEDLFAHFSAIAGKGFKSLKE
                     NQRVDFEVTTGPKGKQASNIRPAAE"
     gene            complement(3637..5709)
                     /locus_tag="VKZ48_08400"
     CDS             complement(3637..5709)
                     /locus_tag="VKZ48_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLU77906.1"
                     /translation="MPQLYVYAAAVVLLCALAAVAWRLRSALRETARRLDEAERWISG
                     VRLANEAAAVAVWEWDLDHDRMRGDEQLAHLFGVPAGYAPASLAELLADVHPADAEAL
                     CAHMNAARETNDRVDAEFRVRGEEQSTRYLHLRGACIADSRSRRRLMVGAIWDVSDER
                     AHQLELERYAGHLAAAYATSPVAIFVTTLDGRIVEVNDTAVAMTGYSRQTLSALNVAE
                     LGFWRGQPESFEAFSSRLQKGRVLHRIARFLRQDGMPRDAEISATLIDRINQPHVLYM
                     IEDVTEQRRQSTALRESEERFARIFEISPDPIALVSEDGRIVEANPAFLRFSQYDIDE
                     LRGNTTEALGLWAATACAERFKSYLATHRDAENIESMLRRKNGELAHCQVSWSGFDLA
                     GERYVLLIAHDITELKQRSAEVDRLNASLEKMVQARTAQLVSANRELESFSYSVSHDL
                     RAPLRHISGFASLLGERPSVQSDAEARRLIGVVIRAAGRMGALIDDLLAFSRVSRQQM
                     SLQEVPLDELVRDVVQEFSAAEEGREVQWRIGALPTVRGDRQLLRSVMMNLLDNALKY
                     TRPRPVAVIEIGARERDGMAEIYVRDNGVGFDMQYAKQLFDVFQRLHRQEDFEGTGVG
                     LANVQRIVERHGGRVWAEGEAEQGATFWISLPVSHDKPPVWQPPPTAPERSAKQASSK
                     RYARTGSR"
     gene            5798..6769
                     /locus_tag="VKZ48_08405"
     CDS             5798..6769
                     /locus_tag="VKZ48_08405"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HLU77907.1"
                     /translation="MGLLVDGKWHDQWYDTVGTGGRFIRSDAQFRNWITPDGGAGPSG
                     AGGFKAEAGRYHLYVSLACPWAHRTLIFRTLKGLEAMIGVSVVHPYMGEHGWHFEPGD
                     GVVSDPVCSAKYLYELYLRAQPDYSGRVTVPVLWDLQRDTIVSNESAEIIRMFNTAFD
                     AIGAKAGDYAPTALLPRIDALNAEIYDAVNNGVYKAGFATAQDVYEEEVRNLFACLDT
                     LEARLENQPYLLGEQVTETDWRLFTTLIRFDAVYHGHFKCNLRRLVDYPRLWRYTRRL
                     YHWPGVAATVNFDHIKQHYYRSHPTINPNGVVPLGPELDLTLEDTQR"
     gene            6766..7203
                     /locus_tag="VKZ48_08410"
     CDS             6766..7203
                     /locus_tag="VKZ48_08410"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HLU77908.1"
                     /translation="MRTILAAIIAILFVTGCAQALPQPESATLAGTAWQLVRFQGGDD
                     TLLVPDDKAKYTLRFDADGTVAVRMDCNRGRATWRADPPQLQFGPLVSTRAACPPGSL
                     HDRIVRHWPYVRSYIVQDGHLHLSLMADGGIYEFEPMTEPAGK"
     gene            7281..8246
                     /gene="rssA"
                     /locus_tag="VKZ48_08415"
     CDS             7281..8246
                     /gene="rssA"
                     /locus_tag="VKZ48_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HLU77909.1"
                     /translation="MTQKKNPSTQLALTGTSATRRRKPRVGLALGSGSARGWAHIGVI
                     QALHEAGVRPDIVCGTSIGGLVGAAYATGQLKQFEEWVLTLGVREVVSFMDLGLNGGM
                     LKGERLMAFFRKNFLDRPIEELDMPFGVVATALHSGAEIWLRSGSTVKAVRASIALPG
                     LFTPVKHEGMVLVDGGLVNPVPVSLARAMGAEIVIAVDLSSDLLSRRPHVEVMAQEPA
                     PSGVVSEWMRKLQETFGFASAPEKLDEPLLPSMLDVMATSLNIMQVRITRSRMAGEPP
                     ELVIAPRLAHLGLLDYHRAKEAIEEGRRAVEVGLHSLDLLGLETP"
     gene            8243..8707
                     /locus_tag="VKZ48_08420"
     CDS             8243..8707
                     /locus_tag="VKZ48_08420"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HLU77910.1"
                     /translation="MTERDVPGVIALPPLLYAGALLLVVILERLMPWSLYAHPARVWI
                     GAALTVAGIAVVLWGSATMHRAGTNVPPNRPATALVTQGPFRYTRNPLYLGLHATFLG
                     LTYLLQSAWGLLVFVPLAVVMHHGVILREEAYLERKFGDAYRAYCKSVRRYF"
     gene            8766..>9334
                     /locus_tag="VKZ48_08425"
     CDS             8766..>9334
                     /locus_tag="VKZ48_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77911.1"
                     /translation="MRTLLALFLGLVAQAAIASCPPHAYTAPGQVRFDSDAVLIVTHP
                     TSFHDARLASKRGIDEAVSFARNKRIPVVYLQDDTPAKLYFVEDCDPDYWVESEGGEF
                     GLEVTPSHVYVAGGHLELCLSDTLHEILRSWAKQTPRNLTITYLMDAIYSNGKSIEES
                     DPFYADYLKFMDVVTYRRPGGEHWPKLTLL"
BASE COUNT         1702 a         3120 c         2893 g         1619 t
ORIGIN      
        1 tcatcgaagc gcgcggttcg tcttccgccg ccagtgccgc aagcgccgcc atcgatcaca
       61 tgcgcgactg ggtactgggc agcaacggcg aatgggtcac catgggcgtg ccgagcgacg
      121 gttcctatgg cattccggaa ggcatcgtct acggctttcc ggtgatctgc gccgatggcg
      181 gctacgagat cgtgcgcgac ctggatgtgg acgatttcag ccgccagaaa atggatgcca
      241 cgctgaaaga actgctggaa gagcgcgacg gcatcaagca tctcttgagc tgatcgacgc
      301 gcgaggccgg ccgacgcgca gtcgccggct cgccgcctac gcattgctct gccctcgtca
      361 cttttgatcc cggctcacgg ctcatacgac atgccccata accccttcga caccctgcaa
      421 gaattcaagc ccggtaaagg caagaccggg cgcttctatt ccctgccgac gctgcagcgc
      481 acaggtttcc ccaatctctc gcgactgccc gtttcgatcc gcatcgtgct ggaagcggtg
      541 ttgcgcaacg tggacggcca gaaggtcacg gcggaccacg tgcgtcagct cgccaagtgg
      601 cagccgaacg ccgcgcgaac ggaagagatt cccttcgtcg tggcgcgcat cgtgctgcag
      661 gacttcaccg gcgtgcccct gctggccgac cttgcggcga tgcgcggcgt ggccgatcgc
      721 atgggcaagg accccaagct gatcgagccg ctcgtgccgg tcgatctggt ggtggaccac
      781 tcggtgcagg tcgacagcta cggcaacccg aacgcgctcg acatcaacca gaagttggaa
      841 ttcaagcgca accgcgagcg ctaccagttc atgaaatggg gcatgcaggc tttcgacacc
      901 ttcaaggtcg tgcctccggg tatcggcatc gtgcaccagg tgaacctgga atatctcgcg
      961 cgcggcgtgc accagcagga cggcctctac tatcccgaca cgctggtcgg caccgatagc
     1021 cacacgacca tgatcaatgg catcggcgtc gtgggctggg gcgtcggcgg catcgaggcc
     1081 gaggccggca tgctcggaca gccggtctac ttcctcacgc ccgatgtggt cggcgtgcat
     1141 ctcaaaggca gcctgcgcgg cggcgtgacc gccaccgacc tcgtgctgac catcacggaa
     1201 atgctgcgca aggcgaaagt ggtaggcaag ttcgtcgagt tcttcggcga aggcgtggag
     1261 aacctctccg tgcccgatcg cgccaccatc ggcaacatgg cgccggagta cggcgcgacc
     1321 atcggcttct tcccggtgga cgacaagacc gtggcctatt tcgaggacac cggccgcacc
     1381 aaggaggaaa tcgaggcctt ccgcgcctac ttcaaagcgc aggagttgta cggcattccg
     1441 aaactcggcg acatcgacta cagcgaagtg ctggaactcg atctgtccaa ggtcgcacct
     1501 tcgctgtccg gcccgaagcg cccgcaggac cggatcgaaa tcggcgacgt gaaggcgcag
     1561 ttcaccaaac ttttttcggc gccgacgtcc cagaacggat ttgccaagaa ggcggaagac
     1621 ctgcccaagc gctatacgac caaggacggc atcgacatcg gttcgggcga cgtgctgatc
     1681 gccgccatca cctcctgcac caacacgtcc aatccgggcg tgctgctcgc cgccggcctg
     1741 ctggcgaaga aagccgtcga gcgcggcatg accgtcaagc cgcacatcaa gacctcgctt
     1801 gcccccggct cgcgcgtcgt caccgaatat ctgcgcaagg cgaagctgct gccctacctg
     1861 gagaaactgg gcttttatct ggcgggctac ggttgcacga cctgtatcgg caactccggg
     1921 ccgctggccg agccgatcga agaagcgatc gtcgatcacg atctggtctg cgcggctgtg
     1981 ctgtccggca accgcaactt cgaggcgcgc atccacagca acattcgcgc gaacttcctc
     2041 gcctcgccgc ccatggtcgt cgcctacgcc ctggccggca cgatgctgaa agatctcatg
     2101 accgagccgg tcggcaaggg caaggacggc gaggacgtgt ggatcggcga catctggccg
     2161 accagcgagg aaatctacga gtgcatgaag cacgcgaaga atccgagcac cttccgcaag
     2221 ctgtacaccg acctggccga acagaacccg ttgtggaagg agatcggcgc gaccgccggc
     2281 caggtgtacg actggccgcc gtccacctac atcgccgaac cgcccttctt cgagggcttc
     2341 ggcatgcagc ccggccggat cggcgacgtg cacggcgcgc gcattctcgc cctgttcggc
     2401 gactccatca ccaccgatca catcagtccc gccggggcca tccaggagac ctcgcccgcc
     2461 gggcagtacc tcatcaagca cggcgtgttg aagcccgact tcaacagcta cggcgcgcgt
     2521 cgcggcaatc atgaagtgat gatgcgcggc accttcgcca acgtgcgcat caagaacctc
     2581 atgctgccgc cgaaggaaga cggctcgcgc gtcgagggcg gctacacgct gtttcagccg
     2641 agcggcgaac gaatggcgat ctacgatgcg gccatgcgct acatcgaatc gggtacgccg
     2701 acggtcgtct tcgccggcga ggaatacggc agcggttcgt cgcgcgactg ggcggccaag
     2761 ggcacgcaac tgctcggcgt caaggcggtg atcgcgcgca gcttcgaacg catccatcgt
     2821 tcgaacctcg tcggcatggg cgtgctgccc tgccagttca tcggcaacga ttcggtggaa
     2881 acgctgggcc tgaaaggcga cgaagtcatc gacctgctcg gcgtggaagg cgaactgcgg
     2941 ccgcagcagg agttgaccct gatcatcaag tccgcgaacg gcgaacagcg cagcatcaag
     3001 gtgctgctgc gcatcgacac gccgatcgaa gtggactact accggcacgg cggcattctg
     3061 cccttcgtgc tgcgagaact gctggcggcg taagaggcag ccgggagcgg ttcgacgctt
     3121 tacgcttact ctcggctccc ggccgagcca aaacaaaaag gccgtcgcct cccggcgacg
     3181 gccttttgcg ttccagacag ccgattactc ggcggcggga cggatgttcg aagcctgctt
     3241 gcccttgggg cccgtcgtca cttcgaaatc cacacgctgg ttttccttca gactcttgaa
     3301 tcccttgccc gcgattgcgg agaagtgagc gaacaaatct tcgccacctc catccggggt
     3361 gatgaatccg tagcccttct ggtcgttgaa ccatttgacg atgccggatg ccatgcttac
     3421 tgctcctgtg aactgaggtg ctgccttgtt tacaaaacgg gcttgcgccc acctgcaaga
     3481 ccttcaagga gtctgacacc acgacttgca gttgccgcag cgcgcggcgt ctacggacca
     3541 gcgattgctc accctgcttg agtctgcagc gaaatttata ctgccggggt atgggggcgt
     3601 caacacagtt ttacagtttg tgacgggcgc aacgcctcag cgcgagcccg tgcgggcata
     3661 tcgtttcgac gacgcctgct tcgccgagcg ctccggtgca gtcggcggcg gctgccagac
     3721 cggcggcttg tcgtgggaaa ccggcaagct gatccagaag gtcgcgccct gctccgcctc
     3781 gccctcggcc catacacgac cgccgtgccg ctcgacgatg cgctggacgt tggccagtcc
     3841 cacacccgta ccctcgaaat cctcctgccg atgcagccgc tggaagacgt cgaagagctg
     3901 cttcgcatac tgcatgtcga agcccacgcc gttgtcccgg acgtagatct cggccatgcc
     3961 gtcgcgctcg cgcgcgccga tctcgatgac ggcgaccggc cgcgggcggg tgtacttcaa
     4021 tgcgttgtcc aacagattca tcatgacgct gcgcagcaac tgacggtcgc cgcgcacggt
     4081 cggcaacgca ccgatgcgcc attgcacttc gcgcccttcc tcggcagcgc tgaactcctg
     4141 caccacatcg cgcaccagtt cgtccagcgg cacctcctgc agcgacatct gctgacgcga
     4201 tacgcgggag aaggcgagca gatcgtcgat gagcgcaccc atgcgccccg cggcgcggat
     4261 gaccacgccg atgagccggc gcgcctccgc atccgactgc accgacggcc gctcgccgag
     4321 cagcgaggcg aagccggaga tatgccgcaa cggcgcgcgc agatcgtgcg acacggaata
     4381 ggagaaggac tccagttcgc ggttcgcgct caccagttgc gccgtgcgcg cctgcaccat
     4441 cttttccagc gaggcgttga ggcggtcgac ttccgcagag cgctgcttca gttcggtgat
     4501 gtcgtgcgcg atcagcagga cgtagcgctc gccggccaga tcaaaaccgc tccacgacac
     4561 ctggcaatgc gcgagttcgc cgttcttccg gcgcagcatg gattcgatgt tctccgcatc
     4621 gcgatgcgtg gccaggtagc tcttgaagcg ctcggcacac gccgtagccg cccacagccc
     4681 cagcgcttcc gtcgtgttgc cgcgcagctc gtcgatgtcg tactgcgaga agcgcagaaa
     4741 ggcgggattc gcctcgacga tgcggccgtc ttccgatacc agcgcaatcg gatcgggcga
     4801 aatctcgaat atccgtgcaa agcgttcctc gctttcgcgc agcgcggtcg actgccgccg
     4861 ctgctcggtc acgtcttcga tcatgtacag cacgtgcggc tggttgatgc ggtcgatcag
     4921 ggtggcggaa atctcggcat cgcgcggcat cccgtcctgc cgcaggaaac gggcgatgcg
     4981 atgcagcacg cgccccttct gcagtcgcga ggagaaggct tcgaaggact ccggctggcc
     5041 gcgccagaaa cccagctccg ccacgttgag tgccgacagc gtctgacgcg aataaccggt
     5101 catggccacg gccgtatcgt tgacttcgac gatgcggccg tcgagggtgg tgacgaatat
     5161 cgcgacggga gaagtcgcat acgccgcggc cagatggccg gcgtagcgtt ccagttccag
     5221 ttggtgggcg cgctcatcgc tcacgtccca gatcgcgccc accatcaggc gccggcgcga
     5281 gcgcgaatcc gcgatgcacg cgccgcgcag atgcagatag cgcgtgctct gctcctcgcc
     5341 cctcacgcgg aactccgcat cgacgcgatc gttggtctcg cgcgccgcgt tcatgtgcgc
     5401 gcacaacgct tcggcatcgg ccggatgcac atcggcaagc agttcggcca gcgatgcggg
     5461 cgcgtaaccg gccggcacac cgaacaggtg cgcaagctgt tcgtccccgc gcatgcggtc
     5521 gtggtcgaga tcccattccc acaccgccac ggctgctgcc tcattggcca ggcgcacgcc
     5581 ggatatccaa cgctccgcct cgtcgagacg gcgcgcggtt tcgcgcagcg cggatcgcag
     5641 ccgccaggca acagcggcca gcgcgcatag cagcaccacg gctgccgcgt agacgtaaag
     5701 ctgtggcatg gtagggggga ggagcaccga tcggtctcgg atggcccacg catgcaataa
     5761 acaggccgcg tggcacacac tcaagcggag aatcggtatg ggattactgg tcgacggcaa
     5821 atggcacgat caatggtacg acaccgtcgg gacgggcggc cgcttcatcc gcagcgatgc
     5881 gcagttccgc aactggatca cgccggacgg cggcgcaggt cccagcgggg ccggcggctt
     5941 caaggccgag gccggccgct accatctcta tgtgtcgctc gcctgcccgt gggcgcatcg
     6001 cacgctgatt ttccgaacgc tgaagggact ggaagccatg atcggcgtct ccgtcgtgca
     6061 tccgtacatg ggcgaacacg gctggcattt cgaaccgggc gacggcgtcg tgtccgatcc
     6121 ggtctgcagc gcgaaatatc tgtacgaact ctatctgcgc gcgcagcccg actacagcgg
     6181 acgcgtcacc gttccggtgc tgtgggattt gcaacgcgac accatcgtga gcaacgagtc
     6241 ggccgagatc atccgcatgt tcaacacggc cttcgatgcg atcggcgcaa aggcgggcga
     6301 ttacgcgccg acggctttac ttcctcgcat cgatgcgctc aacgcggaaa tctacgatgc
     6361 ggtgaacaac ggcgtgtata aggccgggtt cgccacggcg caggacgtgt acgaggagga
     6421 agttcgcaac ctgttcgcct gcctcgatac gctcgaagcc cggctggaaa accagcctta
     6481 tctgctgggt gagcaggtca ccgaaaccga ctggcgactg ttcactacgc tgatccgctt
     6541 cgatgccgtg taccacggcc atttcaaatg caatctcagg cggctcgtcg actacccccg
     6601 cctgtggcgc tacacgcgcc gcctctatca ttggcccggc gtcgccgcaa cggtgaactt
     6661 cgatcacatc aagcagcatt actaccgcag tcatccgacc atcaatccca acggcgtcgt
     6721 gccgctcggc cccgaactgg atctgaccct cgaagacacg cagcgatgag aacgatcctt
     6781 gccgcgatca tcgccatact gttcgtgacc ggctgcgcgc aagcgctgcc gcaacccgag
     6841 tcggccaccc tcgccggcac ggcgtggcaa ctcgtgcgat tccaaggcgg cgacgacacg
     6901 ttgctcgttc ccgacgacaa ggcgaaatac acgctgcgct tcgacgcgga cggcacggtc
     6961 gctgtgcgca tggattgcaa ccgcggccgt gccacctgga gggccgatcc gccgcaactg
     7021 cagttcggac cgcttgtgtc gacgcgtgcg gcctgcccac ccgggtcgct gcacgaccgc
     7081 atcgtcaggc actggccgta cgtgcgctcc tacatcgtgc aagacggcca tcttcatctt
     7141 tcgttgatgg ccgacggcgg catctacgag ttcgaaccga tgacggaacc ggcggggaag
     7201 taatgctgca ttggccgcac cgccgccttg cctgcgcgga cgggcgcgtc ttatgctctt
     7261 cgacgtccgt ggggagcaac atgacacaga aaaagaaccc gagcacacaa ctcgccctga
     7321 ccggaaccag cgcgacccgg cggcgcaagc cgcgcgtcgg gctggcgctg ggcagcggct
     7381 cggcacgcgg ctgggcacat atcggcgtca tccaggcctt gcacgaagcg ggcgtccgtc
     7441 ccgacatcgt gtgcggcacc tcgatcggcg gcctcgtcgg cgctgcctat gcaacgggac
     7501 aactgaagca attcgaggaa tgggtgctca cgctgggcgt gcgtgaagtg gtctcgttca
     7561 tggatctcgg cctcaatggc ggcatgctca aaggcgagcg gctgatggcc ttcttccgca
     7621 agaactttct ggaccgcccg atcgaggaat tggacatgcc tttcggcgtg gtcgccaccg
     7681 ccttgcacag cggtgccgaa atctggctgc gctcgggctc cacggtcaag gcggtgcgcg
     7741 cgtcgatcgc cctccccggc ctgttcacgc ctgtgaagca cgaaggcatg gtgctggtgg
     7801 acggcggact ggtgaatccg gtgccggtgt ccctggcacg ggcgatgggc gccgaaatcg
     7861 tcatcgccgt cgatctcagt tccgacctgc tcagccgccg tccgcacgtg gaggtgatgg
     7921 cgcaggaacc ggcgcccagc ggcgtcgtga gtgaatggat gcgcaagctg caggaaacct
     7981 tcggcttcgc cagcgcgccg gaaaagcttg acgaaccgct gctgccttcc atgctcgacg
     8041 tgatggccac cagcctcaac atcatgcagg tgcgtatcac acgcagccgc atggccggcg
     8101 agccgccgga actggtcatc gcgccgcgcc tcgcgcacct gggcctgctc gactaccacc
     8161 gggcgaagga ggccatcgag gaaggccgtc gcgccgtcga agtcggactg cacagtctcg
     8221 acctgctggg cctcgagacg ccatgaccga gcgcgacgtg cccggcgtca tcgcgctgcc
     8281 gccgctgctc tatgccggcg ccctcctgct cgtcgtcatc ctcgaacggc tgatgccctg
     8341 gtcgctgtat gcgcacccgg cccgcgtctg gatcggtgcg gcgctgaccg tcgccggtat
     8401 cgcggtcgtg ttatggggca gcgccaccat gcatcgcgcg ggcaccaacg tgccgcccaa
     8461 ccgccccgcc accgcgctgg tgacgcaggg accgttccgc tacacgcgca atccgctcta
     8521 cctggggctg catgccacct ttctcgggct gacctatctg ctgcaaagcg catgggggct
     8581 gctggtcttc gtgccgctgg cggtggtcat gcaccacggc gtgatcctgc gcgaggaggc
     8641 ttacctcgag cgcaagttcg gcgatgctta tcgagcttat tgcaaatcgg tccgccggta
     8701 tttttgacgc tgcgcgattc acgccggccg tgtaagctgc gccccatttc agcgtccacg
     8761 cccttatgcg taccctgctt gcgctgttcc tcggtctcgt cgcccaagcg gcgatcgcca
     8821 gttgcccgcc gcatgcctac accgctcccg gccaggtacg cttcgacagc gatgcagtgc
     8881 tcatcgtcac ccaccccact tcgttccacg acgcgcgcct cgcctccaaa cgcggcatcg
     8941 acgaggcggt gagcttcgcc aggaacaagc gcattccggt ggtctatctg caggacgaca
     9001 cgccggcgaa gctctacttc gtcgaggatt gcgatccgga ttattgggtg gaatccgaag
     9061 gcggcgagtt cggcttggag gtcacgccct cgcacgtcta tgtcgcgggc ggccatctgg
     9121 aactgtgtct gtccgacacg ctgcacgaaa tcctgcgctc ctgggccaag cagacgccgc
     9181 gcaacctcac catcacctat ctgatggacg caatctactc gaacggcaag agcatcgagg
     9241 aaagcgatcc gttctacgcc gactatctga aattcatgga tgtcgtcacc taccgccgtc
     9301 cgggcggcga gcactggccc aagctgacgc tgct
//
LOCUS       DATGOU010000148         8500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_4426880, whole genome shotgun sequence.
ACCESSION   DATGOU010000148 DATGOU010000000
VERSION     DATGOU010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 8500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8500
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4426880"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VKZ48_08430"
     CDS             <1..192
                     /locus_tag="VKZ48_08430"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HLU77912.1"
                     /translation="EKEAKENGGRILHSERYYGKLSRSFRLSQEIDAAGAQAKYSDGV
                     LELILPKKQAESARRLSIQ"
     gene            295..1182
                     /gene="argB"
                     /locus_tag="VKZ48_08435"
     CDS             295..1182
                     /gene="argB"
                     /locus_tag="VKZ48_08435"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HLU77913.1"
                     /translation="MNVNPQNAAQKAQTLAEALPYIRRFHGKTVVIKYGGNAMTDPKL
                     QRSFAHDVVLLKLIGLNPVVVHGGGPQIEQLLTKLGKKGEFVQGMRVTDAETMDVVEM
                     VLAGAVNKDIVTLINQAGGKAVGLTGQDGGFILARKLLMPNKDKPEEMIDIGQVGEIV
                     AIDASVISSLEQGGFIPVVAPIGVGRDGETYNINADLVAGKLAEVLRAEKLVLLTNTP
                     GVLDKDGKLLTGLTPRRVDELVADGTLSGGMLPKIASALDAARSGVKSVHIIDGRVEH
                     ALLLEIFTDEGVGTLIRNH"
     gene            complement(1196..1672)
                     /locus_tag="VKZ48_08440"
     CDS             complement(1196..1672)
                     /locus_tag="VKZ48_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HLU77914.1"
                     /translation="MLSNPSRRLSQYFTLAQLTRSETARQRGIDNTPPAALLSNLERL
                     AAGLDAVQALLGHPLEISSAYRCPALNEAVGGTAHSQHCQGLAADFVCPGYGEPPAVA
                     QAIATSALDFDQCILEFGRWVHLSFSPTPRRRLLSIYSSREGYLDGLIDAGGTRLA"
     gene            1780..2355
                     /gene="slmA"
                     /locus_tag="VKZ48_08445"
     CDS             1780..2355
                     /gene="slmA"
                     /locus_tag="VKZ48_08445"
                     /inference="COORDINATES: protein motif:HMM:NF007015.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HLU77915.1"
                     /translation="MATRPGERKLQILQTLAAMLEQPAGEKITTAALAARLDVSEAAL
                     YRHFASKAQMYEGLIEFIESSLFGVINKITAEEENGMRQAHAIVALLLNFAEKNPGMT
                     RVLVGDALVHENPRLQARINQLHDRLEATLKQCLRVAAAQGGGSADTDEVAARANLML
                     SYVVGRWHQFAKSGFKRLPTELFEQQWAFLA"
     gene            complement(2407..3174)
                     /gene="moeB"
                     /locus_tag="VKZ48_08450"
     CDS             complement(2407..3174)
                     /gene="moeB"
                     /locus_tag="VKZ48_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HLU77916.1"
                     /translation="MNDLNDHQLLRYSRHILLPEIGIEGQEKIRTARALILGAGGLGS
                     PAAMYLAAAGIGAITLVDDDDVDLTNLQRQILHTVHTVGQTKVESGKTTLARLNPDVR
                     VETVHERVDGARLDELVAQVDIVLDCTDNFATRHALNRACVAHRKPLVSGAAIRFDAQ
                     ISVYDLRRDDSPCYNCLFPEEGETDEMRCAVMGVFAPITGIVGTVQAAEALKLIVGIG
                     ESLCGRVLLLDALTMNWRSFKLKRDPQCKVCGPGSAA"
     gene            complement(3241..4728)
                     /locus_tag="VKZ48_08455"
     CDS             complement(3241..4728)
                     /locus_tag="VKZ48_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HLU77917.1"
                     /translation="MEFFGMESLDKRSRMKGIGLVALGIVTGVLLSLNLSAIAQRDVE
                     SYPLPVEELRAFAEVFGAIKTGYVEPVEDKRLITEAINGMLTGLDPHSAYLDQDAFKE
                     LQVGTQGEFGGLGIEVGMEEGFVKVVSPIEDTPAFRAGLKTGDLIIKIDETPVKGMTL
                     SDAVKRMRGKPKTSIQLTVVRKGETKPLEFTIVRDIIKVRSVKSKVLEPGYGYVRITQ
                     FQEHTGENLVQHLNELYKDGPLKGLVLDLRNDPGGLLNGAVGISAAFLPPKVTVVSTD
                     GRTEDAKRKYVASPEDYLRGTRDDYLRDLPAHVKNVPMVVLVNGGSASASEIVAGALQ
                     DYKRATVIGTPTFGKGSVQTIMPLGNNTAIKLTTAKYYTPKGRSIQAKGITPDIEVEE
                     PGAPSSRIREADLLKHLEAEEKETLERARAATEAEAVKKDEAPVKKGEDAEDKPIELG
                     SPDDFQLQQALNHLKGLPVLARLEKSEDPAKAAAAVAKGAPEAEK"
     gene            complement(4743..5930)
                     /locus_tag="VKZ48_08460"
     CDS             complement(4743..5930)
                     /locus_tag="VKZ48_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HLU77918.1"
                     /translation="MNRRFAAACLALALLYGAAATASEKEALREARERLEQLRRDLSK
                     SESERERAVDQLRASEKAISAANRKLRELQAAQRNAETELKRLTQETAELSDRIADGR
                     ERLSRMLRARYMAGTHTPLKLLLSGEEQGDAARRLTYQAYVSRAQADFIRSLEADVDR
                     LAALRESTEARREELARLEAEQRQERESLDAQRAERRTVLARISDDVRARRKEVEAAR
                     RDEARLARVVDELAKAATARAQARAKATRKGTRNEQVPEAFAADVKFTALKGRLRLPV
                     RGELVNRFGTPRQDSGLIGKGVFIRARAGEEVRAVANGEVVYADWLRGFGNLLILDHG
                     AGYMTIYGHNEAVLKRPGDLVRAGDVVATVGATGGSQTAGLYFEMRHQGRPFDPLSWV
                     KLK"
     gene            complement(5927..6676)
                     /gene="gpmA"
                     /locus_tag="VKZ48_08465"
     CDS             complement(5927..6676)
                     /gene="gpmA"
                     /locus_tag="VKZ48_08465"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HLU77919.1"
                     /translation="MKKLVVLRHGESLWNRENRFTGWADVGLTEKGENEARQAGVLMK
                     EASLQFDVAYTSMLKRAIKTLWLALENMDQMWLPIEHSWRLNERHYGALQGLNKAEMA
                     EKFGEEQVLMWRRSYDVRPPALDPDDPRVVTGDPRYRGVDPADIPLTECLQDTVARLL
                     PLWNGSIAPAINRGERVLITAHGNSLRALVKLLEDMDKDELIELNIPTGVPLVYELTD
                     ELKPIRRYYLGDQSAVQAAAQAVANQGKRSA"
     gene            6784..7194
                     /locus_tag="VKZ48_08470"
     CDS             6784..7194
                     /locus_tag="VKZ48_08470"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HLU77920.1"
                     /translation="MEFLLDNIFYVAVALVSGSMLIWPLLRPQAGCPTISTLDATMRI
                     NKQDAAVIDVRSAESYAEGHLPRARHMPVKELADRLGEIERLKNKPVIITSDNDGGAA
                     ASAAKVLKRHGFSEVLILEGGVSAWRKADLPVEK"
     gene            7214..7456
                     /gene="grxC"
                     /locus_tag="VKZ48_08475"
     CDS             7214..7456
                     /gene="grxC"
                     /locus_tag="VKZ48_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HLU77921.1"
                     /translation="MYMTEWCPYCRRADQLFRAKDVQDIERIRVDLEPERRQEMMEKS
                     GRHTVPQIWIGETHVGGYDDLVALDRAGKLDALLAG"
     gene            7496..7972
                     /gene="secB"
                     /locus_tag="VKZ48_08480"
     CDS             7496..7972
                     /gene="secB"
                     /locus_tag="VKZ48_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011658008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HLU77922.1"
                     /translation="MSEPQQPVFSIEKVYVKDVSLEVPRGAQSFLESGQPEFEVNINT
                     QGERVNETLYEVAVKVTVTAKNGDQALFLVEVEQAGIFQIRNVPESEMNPVLGIACPT
                     ILFPYVRETVADLVGRAGFPPIHLAPVNFEAMYAAAQQARQQNGESGDGPRIEIAH"
     gene            7972..8409
                     /locus_tag="VKZ48_08485"
     CDS             7972..8409
                     /locus_tag="VKZ48_08485"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HLU77923.1"
                     /translation="MARVLVLLLGGALSFGALATDYRSVGQSAAILYDAPSTHARKLY
                     VASRQLPVEIISSDGAWVKVRDPQGDLAWSGRGALSEQRTVVVTAGVADIRQHPDSQS
                     PVVFQAQQGVVLELQASTVPGWLHVRHADGSAGYVRLNQVWGG"
BASE COUNT         1505 a         2681 c         2736 g         1578 t
ORIGIN      
        1 gagaaggaag ccaaggaaaa cggcggccgt atcctgcaca gcgagcggta ttacggaaag
       61 ctgtcgcgca gcttccgtct cagccaggaa atcgatgcgg ccggtgcgca ggccaaatac
      121 agcgacggcg tgctggaact gatcctgccg aagaagcagg ccgaaagcgc aaggcgcctg
      181 tccatccagt aaatccgctt ctcagtcgct cccgcgttcc agggcggccc gtgtggccgc
      241 cctgtttgtt ttgcgggggg ctagaatcgc gtcttccaga ctgaaggcgc atcaatgaac
      301 gtcaatccgc aaaacgccgc gcaaaaagcg cagacgctgg ccgaggcgtt gccctatatc
      361 cgccgctttc acggcaagac cgtcgtgatc aagtacggcg gtaacgccat gaccgatccg
      421 aaactgcagc gctccttcgc ccacgacgtg gtgctgctga aactgatcgg cctgaatccc
      481 gtggtggtcc acggcggcgg tccgcaaatc gaacaactgc tcaccaaact cggcaaaaag
      541 ggcgagttcg tgcagggcat gcgcgttacc gacgcggaga ccatggacgt ggtcgaaatg
      601 gtgctggccg gcgcggtgaa caaggatatc gtcaccctga tcaatcaggc cggcggcaag
      661 gcggtcggcc ttaccggtca ggacggcggc ttcatcctcg cgcgcaagct gctcatgccg
      721 aacaaggaca agcccgagga aatgatcgac atcggccagg tgggcgaaat cgtcgccatc
      781 gatgcgagtg tgatctccag cctggaacag ggcggcttca ttccggtggt ggcgccgatc
      841 ggcgtggggc gggacggcga gacctacaac atcaacgccg acctggtcgc cggcaaactc
      901 gccgaagtgc tgcgcgcgga gaagctcgtg ctgctgacca atacgcccgg cgtgctggac
      961 aaggacggta agctgctcac cgggttgacg ccccgacgcg tggacgaact ggtggccgac
     1021 ggtacgttgt ccggcggcat gctgccgaag atcgcatccg ccctggacgc ggcgcgcagc
     1081 ggcgtcaaga gcgtgcacat catcgacggc cgggtcgagc acgcgctgct actggaaatt
     1141 ttcaccgacg aaggcgtcgg tacgctgatt cgcaatcatt aacggcgtcc ggcgctcagg
     1201 ccagccgcgt gccgcccgcg tcgatcagcc cgtcgaggta gccctcgcgg ctgctgtaga
     1261 tgctcagcag ccgtcgacgg ggcgtcgggc tgaacgacag atgcacccag cggccgaatt
     1321 ccagaatgca ctgatcgaaa tccagagcgg atgtcgcgat ggcctgcgcc acggcaggcg
     1381 gctcgccgta acccgggcag acgaaatcgg ctgccaggcc ctggcagtgc tgcgagtgcg
     1441 ccgtgccgcc gacggcctcg ttgagggcag ggcaacggta ggcgctggaa atctccagag
     1501 gatggccgag cagcgcctgg acggcatcca ggcccgccgc caatcgctcc agattggaaa
     1561 gtagcgccgc cggcggcgta ttgtcgatcc cccgctgtcg ggccgtttcg gagcgggtca
     1621 gttgtgccag cgtgaaatac tgcgacagtc ggcgcgacgg attgctcagc attgcgacat
     1681 actcggcgtt tcgccctcta tcgtgtaggc aagtgcttac ctattggtcg gaagtacaat
     1741 taaaatcgcc ggcataacaa ggcatacggg aggcggcgca tggcaaccag gcccggcgaa
     1801 cgcaagctgc agattctaca aacccttgcc gccatgctgg aacagccggc cggcgagaag
     1861 atcaccacgg ctgccctggc ggcccggctc gacgtgtcgg aggcggcact ctaccggcat
     1921 ttcgccagca aggcgcagat gtacgagggg ctcatcgagt tcatcgaatc gagcctgttc
     1981 ggcgtgatca acaaaattac ggccgaggaa gagaacggca tgcgccaggc gcacgccatt
     2041 gtcgccctgc tgctcaattt cgccgaaaaa aacccgggta tgacgcgcgt gctggtgggc
     2101 gatgctctgg tgcacgagaa cccgcgcctg caggcgcgta tcaaccagtt gcacgatcgg
     2161 ctggaagcga cgttgaaaca atgcttgcgc gtcgcggcgg cgcagggcgg cggatctgcc
     2221 gacacggacg aggtcgcagc acgcgccaac ctcatgctga gttatgtggt cggccgctgg
     2281 catcaattcg ccaaaagcgg cttcaaacgt ctgcccaccg agctattcga gcaacagtgg
     2341 gcgttcctcg cctagcccgg ctgcgctgac ttccgcgatg gggagcgccg gtcgtgacgc
     2401 cggcgctcac gcagcgctgc ccggtccgca caccttgcat tgcgggtcgc gcttcagctt
     2461 gaacgagcgc cagttcatcg tcagcgcatc gagtaacagc acccgcccgc aaaggctctc
     2521 gccgatgccg acgatgagtt tcagcgcctc ggcggcctgc accgtcccga cgatgccggt
     2581 gatcggcgcg aacacgccca tgaccgcgca gcgcatttcg tccgtttcgc cctcttccgg
     2641 aaacaggcag ttgtagcagg gactgtcgtc gcgacgcaga tcgtacacgc tgatctgcgc
     2701 gtcgaaacgg atggccgccc ccgacaccag cggcttgcga tgcgccacgc aggcgcggtt
     2761 cagcgcgtgg cgcgtggcga agttgtcggt gcagtcgagc acgatatcga cctgcgcgac
     2821 gagttcatcc agacgggccc cgtccacccg ttcgtggacc gtctccacgc gcacgtcggg
     2881 attgaggcgg gcaagggtcg tcttgccgga ttccaccttg gtctgtccga cggtgtgcac
     2941 ggtatgcagg atctggcgct gcaggttggt gagatcgacg tcgtcgtcgt ccaccagggt
     3001 gatggcgccg atgcccgccg cggcgaggta catcgccgcc ggcgagccga gcccgcctgc
     3061 gcccaggatc agcgcgcgcg cggtgcggat tttctcctgc ccttcgatac cgatttccgg
     3121 cagcaggata tgccgcgaat aacgcagcaa ctgatggtcg ttgagatcgt tcatgcggcc
     3181 attaaatcaa aaaaggccgg cggtagcgcc ggcctttttt cgtgtcgaac ccgggcgggc
     3241 ttacttctcc gcctccggtg cgcctttcgc gaccgcggcg gcggccttgg ccggatcttc
     3301 cgatttctcc aggcgtgcca ggaccggaag gcccttgagg tggttcagtg cctgctgcaa
     3361 ctggaagtcg tcgggcgaac ccagttcgat gggtttgtcc tccgcgtctt cacctttctt
     3421 caccggagct tcgtcctttt tcaccgcctc ggcttccgtc gctgcgcggg cgcgttcgag
     3481 cgtttccttt tcctccgctt ccagatgctt cagcaaatcc gcttcacgga tgcggctgga
     3541 aggcgcgccc ggctcctcga cctcgatgtc gggcgtgatg cccttggcct ggatcgagcg
     3601 gcccttgggc gtgtagtact tggccgtggt cagcttgatc gccgtgttgt tgcccagcgg
     3661 catgatggtc tgcaccgagc ccttgccgaa ggttggtgtg ccgatgacgg tcgcgcgctt
     3721 gtagtcctgc agcgcgccgg ccacgatttc cgaagcggag gccgagccgc cgttcacgag
     3781 caccaccatg gggacgtttt tcacgtgcgc cggcaggtcg cgtagatagt cgtcgcgtgt
     3841 gccgcgcaga taatcctccg ggctggcgac atatttgcgc ttggcgtctt cggtgcgccc
     3901 gtcggtggag acgacggtca ccttgggcgg caggaaagcc gccgaaatgc cgactgcgcc
     3961 gttcagcagg ccgcccggat cattgcgcag atcgagcacc agccctttca gcgggccgtc
     4021 cttgtaaagc tcgttgaggt gctgcaccag gttttcgccg gtgtgctctt ggaattgcgt
     4081 aatgcgcaca tagccgtacc cgggctcgag caccttcgat ttgacgctgc gtaccttgat
     4141 gatgtcgcgc acgatggtga attccagggg cttggtctcg cccttgcgca cgacggtaag
     4201 ctggatcgag gtcttgggct tgccgcgcat ccgcttgacc gcgtcggaga gggtcatgcc
     4261 cttcaccggc gtctcgtcga tcttgatgat cagatcgccg gttttcagtc cggcgcggaa
     4321 ggcgggcgta tcttcgatgg gcgaaacgac cttgacgaaa ccttcctcca tgccgacttc
     4381 gatgcccagg ccgccgaact cgccttgcgt gccgacctgc aattccttga atgcgtcctg
     4441 atcgagatag gccgaatgcg gatcgaggcc ggtcagcatg ccgttgatcg cctcggtgat
     4501 cagccgcttg tcttccaccg gctcgacata gccggtcttg atggcaccga agacttcggc
     4561 aaaggcgcgc aactcctcga ccggaagcgg ataagactcc acatcgcgct gggcgatggc
     4621 cgataggttg agcgacagga ggacgcccgt cacgatgccg agggccacga ggccgatgcc
     4681 tttcatcctg ctgcgcttgt cgagcgattc cataccgaag aactccatag ccacgatccg
     4741 ttttacttca gcttgaccca ggacagcgga tcgaaaggcc gcccttggtg acgcatttca
     4801 aagtataagc cggctgtctg gctgccaccg gtcgcgccga cggtcgccac gacatccccg
     4861 gcgcgcacca agtcgccggg gcgtttcagc acagcctcgt tgtggccgta aatggtcata
     4921 taaccggcac cgtgatcgag gatcagcagg ttgccgaaac cgcgtaacca gtccgcatag
     4981 acgacttccc cgttggctac cgcccgtact tcctcgccgg cgcgggcgcg gatgaaaacg
     5041 cccttgccga tcaaaccgct atcctgacgt ggagtgccga agcggttgac gagttccccg
     5101 cgtaccggca gccgcagccg ccccttcaag gcggtgaact tcacgtcggc ggcgaaggcc
     5161 tcgggcacct gctcgttccg cgtccccttg cgagtcgcct tcgcccgcgc ctgggcgcgc
     5221 gcggtggccg ccttcgccag ttcgtccacg acgcgggcga gccgcgcttc gtcgcgccgc
     5281 gctgcctcga cttccttgcg tcgcgcccgc acgtcgtccg agatgcgagc aaggaccgtg
     5341 cgacgttcgg cgcgctgtgc gtcgaggctt tcgcgttcct ggcgctgctc cgcctccagc
     5401 cgggccagtt cctcccggcg cgcctcggtg ctttcgcgca aagccgcaag acggtcgacg
     5461 tcggcctcca agctccggat gaagtccgcc tgcgcgcgcg acacataagc ctgataagtc
     5521 agccgacgcg cagcgtcgcc ctgctcttca ccggacagca gcaacttgag cggcgtgtgc
     5581 gtgccggcca tgtagcgggc gcgcagcatc cgggagaggc gctcgcggcc gtcggcgata
     5641 cggtcggaca gttcggcggt ttcctgcgta agccgtttca gttcggtttc ggcattgcgc
     5701 tgcgccgcct gcaattcgcg cagcttgcga ttggcggcgg aaatcgcctt ttcggaggcg
     5761 cgcagttgat cgactgcacg ctcgcgctcg ctctcgcttt tgctcagatc gcggcgcagt
     5821 tgctccaggc gctcgcgcgc ttcgcgcaag gcttcctttt ccgaggcggt cgcagcggcg
     5881 ccgtagagca gcgccagtgc gaggcacgcg gccgcgaacc ggcgattcaa gccgaacgtt
     5941 tcccctgatt ggcgacggcc tgcgcggcgg cttgcaccgc gctttgatcg cccaggtaat
     6001 agcggcggat gggcttcagc tcatcggtca gttcatagac cagcggcacg ccggtcggaa
     6061 tgttgagttc gatgagttcg tccttgtcca tgtcttcaag gagtttcacc agcgcgcgca
     6121 gtgagttgcc gtgcgcggtg atcagtaccc gttcgccgcg gttgatcgcg ggggcgatgg
     6181 agccgttcca cagcggcagc aggcgagcaa cggtgtcctg caggcattcg gtgagcggga
     6241 tatcggccgg atcgacgcca cgatagcgcg gatcgccggt gacgacgcgc ggatcgtcgg
     6301 gatcgagcgc cggcggacgc acgtcatagc tgcggcgcca catcagcacc tgctcctcgc
     6361 caaatttctc cgccatctcg gccttgttga ggccctgcaa tgcgccataa tggcgctcgt
     6421 tcagacgcca ggagtgctcg atcggcaacc acatctgatc catgttttcc aacgccagcc
     6481 agagcgtctt gatggcgcgt ttgagcatcg aggtgtatgc cacgtcgaac tggagcgatg
     6541 cctctttcat gagcacaccg gcctgacgcg cttcgttctc gcctttttcg gtgaggccca
     6601 cgtcggccca gccggtaaag cggttttccc ggttccagag actttcgccg tggcggagga
     6661 ctacaagctt cttcatggat gcggtggcgg ccttcgaacc cgaattctat aataagcggc
     6721 ttcgccaacc ggcattcggg gcgttgtaac aacatttaaa accacttcag ccacggatcg
     6781 tcgatggaat ttctgctgga caatattttt tatgtcgcgg ttgcactggt cagcggcagc
     6841 atgcttatct ggccgctgct ccgcccgcag gccggttgcc ccaccatttc cacgctcgat
     6901 gcgaccatgc gcatcaacaa gcaggatgct gcggtgatcg acgtgcgttc cgccgaatcc
     6961 tatgccgaag gccatcttcc gcgcgcacgg catatgccgg tcaaggaatt ggcggaccgt
     7021 ctgggcgaga tcgaacggct gaagaacaag ccggtcatca tcacgagcga taacgacggc
     7081 ggtgcggcag ccagcgcggc gaaagtgctg aagcggcacg gcttcagcga ggtgctgatt
     7141 ctggaaggcg gcgtttccgc atggcggaag gccgacctgc cggtggagaa gtgacgcggt
     7201 ggccagaatc cgcatgtaca tgaccgagtg gtgcccctac tgcaggcgcg ccgatcaact
     7261 gttccgcgcc aaggatgtgc aggatatcga gcgcattcgc gtcgacctcg aacccgaacg
     7321 gcgacaggaa atgatggaaa agagcggccg gcataccgtg ccgcaaatct ggatcggcga
     7381 gacgcatgtc ggcggctacg acgatctggt cgcactggac cgggcgggga agctcgacgc
     7441 cctgctggcc ggctgagtgc cgactccaca ccgaagcaat caatcaggag ctttcatgag
     7501 cgagccgcaa caacccgtat tcagcatcga aaaggtgtac gtcaaggatg tatcgctgga
     7561 agtgccgcgc ggcgcgcagt cctttctgga atccggccag ccggaattcg aggtgaacat
     7621 caatacgcag ggcgagcgtg tcaacgagac gctttacgaa gtggcggtaa aagtgaccgt
     7681 cacggccaag aacggcgatc aggcgctgtt tctggtggag gtcgagcagg ccggtatttt
     7741 ccagatccgc aatgtgccgg aaagcgaaat gaatcccgtg cttggcatcg cctgcccgac
     7801 catcctgttt ccctatgtgc gcgaaacggt ggcggacctg gtggggcgcg ccggttttcc
     7861 gcccattcac ctggcgccgg tcaacttcga agccatgtat gcggccgccc agcaggcgcg
     7921 gcagcagaac ggcgaatcgg gcgacggccc gcgcatcgag atcgctcact gatggcgcga
     7981 gtgctcgtcc tgctgctcgg cggcgcgctg tccttcggcg cactggctac ggactaccgt
     8041 tccgtcggtc agagtgcagc gatcctgtac gacgcgccgt cgacccacgc gcggaagctc
     8101 tatgtcgcaa gccgccagtt gccggtggaa atcatctcga gcgacggcgc ctgggtcaaa
     8161 gtgcgtgacc cgcagggtga tctcgcgtgg agcggacgcg gcgcattgtc cgaacagcgc
     8221 acggtcgtcg tcacggccgg tgtggcggac atccgtcagc atccggattc gcagtcgccc
     8281 gtcgttttcc aggcgcagca aggcgtggtg ctggaactgc aggcgtccac cgtgccgggc
     8341 tggctgcacg ttcggcatgc agacggtagc gccggttacg tacgactcaa tcaggtgtgg
     8401 ggcggctgat atcaggccgc tttccgccgg gttgtcacgc ggaatgccat gaatattctg
     8461 attctcggcg cgggcgcctg gggcaccgcc ctggccatcg
//
LOCUS       DATGOU010000149         3441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Burkholderiales bacterium isolate SMAG_U10377
            k141_3552344, whole genome shotgun sequence.
ACCESSION   DATGOU010000149 DATGOU010000000
VERSION     DATGOU010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37957152
            Sequence Read Archive: SRR5690606
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Burkholderiales bacterium (soil metagenome)
  ORGANISM  Burkholderiales bacterium
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 18:00:20
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,702
            CDSs (total)                      :: 1,679
            Genes (coding)                    :: 1,665
            CDSs (with protein)               :: 1,665
            Genes (RNA)                       :: 23
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 14
            CDSs (without protein)            :: 14
            Pseudo Genes (ambiguous residues) :: 0 of 14
            Pseudo Genes (frameshifted)       :: 1 of 14
            Pseudo Genes (incomplete)         :: 12 of 14
            Pseudo Genes (internal stop)      :: 1 of 14
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Burkholderiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3552344"
                     /isolate="SMAG_U10377"
                     /isolation_source="soil"
                     /db_xref="taxon:1891238"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="41.7655 N 111.8143 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VKZ48_08490"
     CDS             <1..212
                     /locus_tag="VKZ48_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77924.1"
                     /translation="RREEHELAYLTKVLPHYENTLSPAYRVELVLNDSVAKVLQSAPG
                     WKPPVLRFHFVDSAVELANEPPHDR"
     gene            223..741
                     /locus_tag="VKZ48_08495"
     CDS             223..741
                     /locus_tag="VKZ48_08495"
                     /inference="COORDINATES: protein motif:HMM:NF042760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HLU77925.1"
                     /translation="MNQTPHRRRFIRHAALGAGAALFGVALPLSAARAAANVDTLLLS
                     CMDFRLMDDIERYMGDRGLRNNYDHIVLAGASLGAVTDKYPAWNRTFWDHLEVAIQLH
                     KVGRLMVIDHRDCGAYKVILGADLHRTPARETDVHAEQLRKLRDLVRQKFPALTVELL
                     LMDLQGKVETLS"
     gene            823..2253
                     /gene="zwf"
                     /locus_tag="VKZ48_08500"
     CDS             823..2253
                     /gene="zwf"
                     /locus_tag="VKZ48_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HLU77926.1"
                     /translation="MLWSLSSRHEEFNRMTQTHHSDALVLFGATGDLAAKKIFPALVT
                     LVRRGKLKCPVIGVAREALSDEDFRARVHEALRDRSEADAAAVEHLCSLLRYVGGDYR
                     DVITYHTLRRRLEGCEHPLYYLAIPPAMFPVVIESLGGSSNVRESRVIVEKPFGRDLP
                     SSQELSATLERVFDENDVFRIDHYIGKEPVQNLLYFRFANTFLEPVWRRGHVRSVQIT
                     MAERLGVGSRGPFYEEVGAMRDVVQNHLLQVVSILAMEPPADDTLQSLQAAKIAVLHA
                     ARPLRAEDVVRGQYDGYRHERGVAPDSEVETYVALRMHIDSARWAGVPFYIRAGKELP
                     VTATEVIVELEPSTSPMFREQAGQQKLGNYFRFRLGPDRVAIAVGARIKSHGEQMRGE
                     AVELFALNARGEEPDAYERLIGDAMRGDHSLFARRESVEASWRIVDPILANPPPVHIY
                     SRGSWGPAIADRLIAGTCRWYAPRTH"
     gene            complement(2312..3055)
                     /locus_tag="VKZ48_08505"
     CDS             complement(2312..3055)
                     /locus_tag="VKZ48_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006575557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HLU77927.1"
                     /translation="MAKSTQMGMNRTGAQMSPKDVKQMQQDAGQLMQLQDTQVQVGSE
                     FDAIEMRLKTIQEADPVGSVPLPGTMKGAMKAGMQKLSGRNAEVFLDKLGERLAYERT
                     GVRLYEAFIVKCRADAQPGQVNLSIDQLLHIHNEEARHFKLLSDAMEKLGADPTAMTP
                     CANTTAVQSIGILQVLTDPRTTIAQCLNAMLTVELTDNAAWELLIQLANEMGQDEMAA
                     QFEEALASEEEHLAIIKQMLQDAVIAEAG"
     gene            complement(3088..>3441)
                     /locus_tag="VKZ48_08510"
     CDS             complement(3088..>3441)
                     /locus_tag="VKZ48_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLU77928.1"
                     /translation="RIKATWERFFDYEMGHFHMVADIYKNTLRRDPQELFPKAFDKRI
                     EFVSHRDYVKKVLQEEVDLRALGTQFIRDKDGESERTLDYRHHLNSEGSPSDAASAGY
                     QWAPGTELAPKVVNL"
BASE COUNT          591 a         1151 c         1051 g          648 t
ORIGIN      
        1 tccgacgtga ggaacacgaa cttgcctacc tcaccaaggt gctgccgcat tacgagaaca
       61 ccctgtcccc ggcctatcgt gtcgagctgg tgctgaacga ctcggtcgcc aaagtgctgc
      121 aaagcgcgcc gggatggaag ccgcccgtgt tgcgcttcca cttcgtcgat tccgccgtgg
      181 aactggcaaa cgaaccgccg catgatcgct aaggagaaaa caatgaatca gacgccgcat
      241 cgccgccgct tcatccgcca cgccgcgctc ggcgcgggcg ccgccttgtt cggcgtcgcg
      301 ctcccgctct ccgccgcgcg cgccgccgcc aacgtcgata cgctgctgct tagttgcatg
      361 gacttccgcc tgatggacga catcgagcgc tacatgggcg atcgcggcct gcgcaacaac
      421 tacgatcaca tcgtcctcgc cggcgcatcg ctgggcgccg tgaccgacaa atatccggcg
      481 tggaaccgca ccttctggga ccacctcgag gttgccatcc agttgcacaa ggtcggccgc
      541 ctgatggtga tcgatcatcg cgattgcggc gcctacaagg tcatcctcgg tgcggacctg
      601 caccggacgc ccgcacgcga aacggacgtc cacgccgagc agttgcgcaa gctacgcgat
      661 ctcgtgcggc agaagttccc cgcgctgacg gtagaactgc tgctcatgga tctgcagggc
      721 aaggtcgaaa ccctctcctg aggctgcatc cgggcacggc ggttgtatcg cccccgctgc
      781 ccgatccgct ctgtgcggct gcgccgcgcg cgaccgggct gtatgctttg gtctttgtcc
      841 agccgccacg aggagttcaa ccgcatgacg cagacccatc actccgacgc cctggtgctg
      901 ttcggtgcaa cgggtgatct cgccgcgaaa aagatttttc ccgccctggt cacactggtg
      961 cgccggggga agctgaaatg ccccgtgatc ggcgttgcac gcgaggcgct aagcgacgag
     1021 gacttccgcg cgcgcgtgca cgaagcgctg cgcgatcgca gtgaagcgga cgccgccgcg
     1081 gtcgaacatc tgtgcagcct gctgcgctac gtcggcggcg actaccgcga cgtcatcacc
     1141 tatcacacgc tgcgccgcag actggaaggc tgcgagcatc cgctctacta cctcgccatt
     1201 ccgccggcga tgttccccgt cgtgatcgaa agcctgggcg gcagttccaa tgtgcgtgaa
     1261 tcgcgcgtca tcgtggagaa acccttcggc cgcgatctgc cctcctcgca ggaactgagc
     1321 gcgacgctgg agcgcgtgtt cgacgagaac gatgtgttcc gcatcgatca ctacatcggc
     1381 aaggagccgg tgcagaacct gctctacttt cgtttcgcca acaccttcct ggaaccggtg
     1441 tggcgacgcg gccatgtgcg cagcgtgcag atcaccatgg ccgagcggct gggcgtaggc
     1501 agccgcggtc cgttctacga ggaagtcggt gccatgcgcg acgtggtgca gaaccacctg
     1561 ctgcaggtcg tatcgatcct cgccatggaa ccgcccgccg acgacacgct gcaaagcctg
     1621 caggcggcga agatcgcggt gctccacgcc gcgcggccgc tgcgcgccga agacgtggtc
     1681 cgcggccagt acgacggcta ccgccacgag cgaggtgtcg cgcccgattc ggaggtggaa
     1741 acttatgtgg cgctgcgcat gcacatcgac tcggcgcgct gggcaggcgt gcctttctac
     1801 attcgcgcag gcaaggaact tcccgtcact gccacggaag tgatcgtgga actggagccc
     1861 tcgacttcgc ccatgttccg cgagcaggcg gggcagcaga aactcggcaa ctacttccgc
     1921 ttccgtctcg gccccgaccg ggtggccatc gccgtcggcg cgcgcatcaa gtcgcacggc
     1981 gaacagatgc gcggcgaggc ggtggaactc tttgcgttga acgcccgcgg agaagagccg
     2041 gatgcctacg agcggttgat cggcgacgcc atgcgcggcg atcacagcct gttcgcgcgc
     2101 cgcgagagcg tcgaagcctc atggcgcatc gtcgatccga ttctggcgaa tccgccgccc
     2161 gtccacatct acagccgcgg gtcctgggga ccggccatcg ccgaccggtt gatcgccggc
     2221 acctgccgct ggtacgcgcc gcgcacgcac taagcgcacg cagaaatgca aacggcccgc
     2281 aagcgggccg tcttgctcga gagcatcgcg ctcaaccggc ttccgcgatc accgcgtcct
     2341 gcagcatctg cttgatgatg gccaggtgct cctcttccga ggcaagcgcc tcctcgaact
     2401 gcgccgccat ttcgtcctga cccatttcgt tcgcgagttg gatcaacagt tcccaggcgg
     2461 cgttatcggt cagttcgacc gtcagcatcg cgttgaggca ctgtgcgatg gtggtacgcg
     2521 gatccgtgag aacctgcagg atgccgatgg actgcaccgc cgtcgtattc gcacagggcg
     2581 tcatcgcggt cggatcggcg ccgagttttt ccatcgcatc gctgagcagc ttgaagtgcc
     2641 gcgcctcctc attgtggatg tgcaacagtt gatcgatgga gaggttgacc tgccccggct
     2701 gcgcatcggc acggcatttg acgatgaacg cctcatacag ccgcacaccc gtgcgctcgt
     2761 aggccagacg ctcgccgagc ttgtcgagaa acacttccgc gttgcgcccc gacagtttct
     2821 gcatgccggc cttcatcgcg cccttcatgg tgccgggcag cggcactgag ccgaccgggt
     2881 cggcttcctg gatcgtcttc aaccgcatct cgatcgcgtc gaactcgctg ccgacctgca
     2941 cctgcgtatc ctgcagttgc atgagttgcc cggcatcctg ctgcatctgt ttcacatcct
     3001 tcggcgacat ctgcgcgccg gtgcggttca tacccatttg cgtggacttt gccatgcctg
     3061 tctcctgctt gattcgatga tcggcggtca gagattgacg accttgggcg ccagttccgt
     3121 ccccggcgcc cattgatagc cggcggacgc cgcatcggaa ggcgagccct ccgaattgag
     3181 gtgatggcgg taatcgagcg tgcgttcgct ttcgccgtcc ttgtcgcgga tgaattgcgt
     3241 acccagcgcg cgcaggtcga cttcctcctg caacaccttc ttgacgtaat cgcgatggct
     3301 gacgaactcg atacgcttgt cgaacgcctt ggggaacagt tcctgcggat cgcggcgcaa
     3361 tgtgttcttg tatatgtccg ccaccatgtg aaagtggccc atctcataat cgaagaaccg
     3421 ctcccaggtc gccttgatgc g
//
